Aijrfans14 228

American International Journal of Research in Formal, Applied & Natural Sciences

Available online at http://www.iasir.net

ISSN (Print): 2328-3777, ISSN (Online): 2328-3785, ISSN (CD-ROM): 2328-3793 AIJRFANS is a refereed, indexed, peer-reviewed, multidisciplinary and open access journal published by International Association of Scientific Innovation and Research (IASIR), USA (An Association Unifying the Sciences, Engineering, and Applied Research)

Compatibility of Bradyrhizobium japonicum isolates with agrochemicals V.V.Deshmukh, B.T.Raut, S.S.Mane, R.W.Ingle, M.S.Joshi Department of Plant Pathology, Dr.PDKV, Akola, Maharashtra 444104, India Abstract: Thirty two Bradyrhizobium japonicum isolates were collected from different locations of Vidarbha region of Maharashtra state. All the thirty two isolates were tested for compatibility with commonly used agrochemicals in soybean cultivation. Isolates BJ12, BJ32 and BJ25 were showed compatibility with thiram. BJ 16. BJ 4, BJ5 , BJ23 and BJ27 were compatible to carbendazim and copper oxychloride at all the concentrations and compatible to ridomil MZ even at higher concentration. Tolerance to pre and post emergence herbicides i.e Persuit and Kloben was recorded in BJ2, BJ10, BJ11, BJ12, BJ23 at all the concentrations. All the isolates tolerated the concentrations of pendamthalin except BJ9. All the isolates showed good compatibility with seed dressing insecticides i.e thiamethoxam and imidaclopride at all the tested concentrations.

I. Introduction Soybean (Glycine max (L.)Merrill) has played a significant contribution to yellow revolution in India and as a food plant it forms an important part of routine diet of the people in India. Symbiotic nitrogen fixation (SNF) resulting from mutual beneficial interaction between soybean and soil nodule bacteria provides a significant role of N fertilization. Soybean depends on its symbionts for a large part of its nitrogen requirement and effective growth with dry matter production. Therefore, success of this crop in the country lies on its efficient symbiosis with N2 fixing bacteria.

The fungicides applied to leguminous plants either seed dressing or soil drench may affect the symbiotic relationship and may persist for longer time. A broad variation regarding susceptibility of individual rhizobial strains to agrochemical was found in different rhizobial species, as well as significantly higher susceptibility of fast growing than slow growing rhizobia.

Frequently herbicides toxicity could be higher that inhibit biochemical process common to both plants and microorganisms. Hence, tolerant strains of bioinoculant is essential against recommended herbicide. Use of herbicides for weed control in legume fields has contributed to increased yield and improved quality.

Frequently, herbicides not only affect plant growth but have a detrimental effect on soil microorganisms. The effect depends on the herbicides, its concentration and different weather condition.

II. Material and Methods Thirty two isolates of Bradyrhizobium japonicum were isolated from root nodules of soybean collected from different locations of Vidarbha region of Maharashtra during 2009-010. The isolation was made on YEMA with congored as per standard procedure. All these isolates were then tested for their compatibility with commonly used agrochemicals in soybean cultivation.

Compatibility of Bradyrhizobium japonicum with agrochemicals Different concentration of agrochemicals i.e.fungicides, herbicides and insecticides were mixed with 100 sterilized YEM broth separately and mixed thoroughly, 10 ml broth containing different concentrations of fungicides, herbicides and insecticides were distributed in 10 test tubes. Each test tube was then inoculated with each isolates and one uninoculated tube serve as control. For each isolate and each chemical concentration ten test tubes were used. Inoculated test tubes were then incubated at room temperature, observations were recorded after 24 and 72 hr turbidometrically at 620 nm. Four concentrations of fungicides, three concentrations of herbicides and insecticides respectively were used in the present study.

These agrochemicals were also tested by paper disc method. A Loopful growth of each isolates were inoculated in 10ml YEM broth and incubated for 24hr. after twenty four hr 1 ml broth containing each isolates were poured in sterilized Petriplates. The sterilized YEMA media was then poured in the plates, rotated clockwise and anticlockwise for uniform mixing. different concentrations of each agrochemicals were mixed in the 10 ml sterilized water under aseptic condition. Autoclaved sterilized 5mm disc of Whatman’s filter paper were soaked in each concentrations for 5 minutes. The disc were kept on the solidified YEMA media. Each plate was

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V.V.Deshmukh et al., American International Journal of Research in Formal, Applied & Natural Sciences, 6(1), March-May 2014, pp. 5562

inoculated with four disc containing different concentrations in triplicates, incubated at room temperature. After 72hr, observations on zone of inhibition was recorded.

III. Results and discussion Regarding thiram maximum growth was recorded in BJ 32 (0.321,0.643 optical density) followed by BJ 25 (0.305,0.627) and BJ 12 (0.300,0.622), whereas least growth was recorded in BJ 30 (0.201,0.525) and BJ 8 (0.203,0.525) at 0.3% after incubation of 24 and 72 hr respectively. At 0.45% concentration, BJ 10 showed maximum growth (0.251,0.526) and least growth (0.180,0.455) exhibited by BJ 18.

At 0.6% concentration BJ 1 (0.173, 0.333) and BJ 27 (0.171,0.333) showed highest optical density where as least growth was observed in BJ 30 (0.122,0.283). Similar results were recorded at 0.75%, where BJ 1 showed maximum optical density (0.088, 0.190) and BJ 18 showed least growth i.e. (0.036, 0.118). The results revealed that, BJ 1 and BJ 27 were compatibility even at higher concentration, where as BJ 32, BJ 25 and BJ 12 could sustain lowest concentration. Lennox and Alexander (1981) also reported similar results, that thiram treated seed increase the nodules number, weight and number of pods indicating no harmful effect. Ndeye, et al.(2003) showed that nodulation was not inhibited in field experiment conducted to assess the compatibility of dichlorofenthion thirum (DCT) on common bean also no significant difference was observed on shoot dry weight. Similarly, Bikrol, et al. (2005) reported that pre seed treatment have positive role in amendment of biologically fixed N2 in the soil up to concentration of 10ug/ml thirum.

BJ 8 showed maximum growth i.e. 0.324 and 0.780 at 24 and 72hr respectively with carbendazim followed by BJ 26 (0.284, 0.754), BJ 30 (0.257,0.785) at 0.1% concentration. At 0.15% BJ 4 showed maximum optical density i.e 0.276 followed by BJ 5 (0.273), BJ 27 (0.273) after 24th hr. At 72 hr BJ 14 showed maximum growth 0.760 followed by BJ 18 (0.745), BJ 27 (0.744) and BJ 29 (0.739). At 0.2 and 0.25% concentration similar observations were recorded. Carbendazim at higher concentration has no detrimental effect on growth of Bradyrhizobia as observed with higher optical density. Similar results were reported by Singh, et al. (1999) who estimated the viability of rhizobia (M-1006) against lethal doses of fungicides (Bavistin, Thiram, Streptocycline and Emisan) in respect to nodulation, shoot dry matter and yield in green gram and reported that thiram and bavistin failed to resist rhizobial growth even at their lethal dose.

Regarding copper oxychloride maximum growth (0.223,0.492) was observed in BJ 2 followed by BJ 5 (0.224,0.485) and BJ 27 (0.223,0.490). The least growth was observed in BJ 21 (0.174,0.442) . At 0.37% concentration BJ 17 and BJ 18 exhibited maximum growth (0.262,0.559 and 0.262,0.556) where as BJ 21 exhibited least growth (0.162, 0.459). At 0.5% and 0.62% concentration similar results were recorded, where BJ 17 and BJ 18 showed maximum growth and BJ 21 showed least growth.

At 0.25% concentration of mancozeb, BJ 6 showed least growth i.e. 0.185 and 0.195 at 24 and 72 hr respectively. Remaining isolates were at par with each other in respect to optical density . At 0.05% concentration maximum growth was recorded in BJ 7 (0.234,0.514) at 24, 72hr respectively. All the isolates tolerated the highest concentration of mancozeb. In a field experiment Ram, et al. (1984) reported that the combine inoculation of chickpea seed with Rhizobium followed by dithane M-45 improved plant height and nodule number as compared to Rhizobium and fungicide alone. Also Patel, et al. (1985) reported that green gram seed treated with fungicides and inoculated with Rhizobium spp. The maximum nodulation (22.31) was recorded in dithane M 45.

Among all the isolates tested, none of the isolates showed sensitivity to ridomil even at higher concentration.

All the isolates showed moderate to good growth after incubation of 24 and 72 hr. No differences in growth of all the isolates was influenced by different concentrations of ridomil. Findings of the present studies corroborate the results published by previous workers with different fungicides and rhizobium in respect to compatibility.

A. Compatibility with fungicides (paper disc method) The compatibility of different isolates have also tested through paper disc method. The filter paper disc containing different concentrations were placed in the YEMA plate already inoculated with the isolates. The zone of inhibition was recorded.

Least zone was observed in BJ32 (0.4,0.6,0.6,0.6 cm) followed by BJ27(0.4,0.6,0.6,0.6 cm) , BJ26 (0.4,0.6,0.5,0.5 cm) at all concentrations, where isolates BJ4 and BJ18 showed sensitivity to all concentrations.

Most of the isolates tolerated all concentrations of carbendazim. Where BJ3, BJ5, BJ6, BJ7, BJ14 and BJ 27 showed highest tolerance towards all four concentrations giving minimum zone of inhibition. Among all the isolates BJ32, BJ27, BJ11 and BJ7 showed minimum zone of inhibition and found tolerated to all the concentrations of thiram. Where BJ3, BJ8 and BJ17 showed sensitivity to thiram by giving maximum zone of inhibition than other isolates.

Most of the isolates showed sensitivity upto 3rd concentration. Where isolate BJ4, BJ16, BJ18 and BJ29 showed maximum zone of inhibition upto 3rd concentration. All isolates found tolerant to mancozeb. No significant inhibition of all isolates were recorded. All the isolates grew well at all concentration except BJ4 was observed to be sensitive to all concentration (0.9,1.0,0.8,0.9 cm)

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V.V.Deshmukh et al., American International Journal of Research in Formal, Applied & Natural Sciences, 6(1), March-May 2014, pp. 5562

The compatibility of Rhizobium with four fungicides i.e. benlet, bavistin, sicarol, dithane Z-78 during application to cowpea seed studied by Narayana, et al. (1984) they concluded that the seed treated with chemicals had no adverse effect on native rhizobia. Mallik and Tesfaic (1985) also reported that pesticides did not affect shoot and root, nodulation even at 10 times recommended concentration of PCNB. Diatloff (1986) showed that no inhibition of Rhizobium japonicum growth was produced when seeds were treated with fungicides (Metalaxyl, Benolaxyl, Captafol, Iprodion) similarly, Singh and Lodha (1997) observed the compatibility of five chemicals seed protectants viz., thirum, dithane M-45, captan, ridomil MZ and bavistin with Bradyrhizobium japonicum in soybean and reported that ridomil MZ and bavistin were found to be compatible. Ndeye Fatou et al. (2003) reported that thiram and bavistin failed to resist rhizobial growth even at their lethal dose. The findings reported by Muthomi, et. al. (2007), Kaur, et al. (2007) and Zilli, et. al. (2009) are also on the similar line of present investigation.

B. Compatibility with herbicides Application as pre and post emergence herbicides is a general practice in soybean growing area for management of weeds. Hence, to determine the effect of herbicides on growth and survival of Bradyrhizobium janponicum with three different concentrations were tested.

Pursuit at all concentrations did not show any negative effect on the growth of isolates. Isolates BJ 12 (0.202,0.489), BJ 11 (0.198,0.485) and BJ 2 (0.191,0.478) recorded maximum growth as observed turbidometrically. At 0.25% concentration BJ 10 (0.184,0.482) and BJ 23 (0.186,0.484) showed maximum growth while BJ 7 exhibited least growth (0.100,0.398) as compared to other isolates. At 0.3% concentration BJ 4 achieved maximum growth (0.269,0.428).

Recommended concentration of kloben (0.08%) did not affect the growth. Maximum growth was recorded in BJ 12 (0.252,0.452) followed by BJ 2 (0.241,0.441) after 24 and 72 hr respectively. Similar observations and growth pattern was observed when these isolates were exposed to 0.12% at 24 and 72 hr. The lowest growth was recorded in BJ 6 (0.073,0.163) while the maximum growth (0.172, 0.262) was recorded in BJ 12 after 24 and 72 hr of incubation at 0.2% concentration.

Regarding pendamethalin, initial growth was quite slow but most of the isolates achieved optimum growth after 24 and 72 hr at all concentrations. At 0.66% isolate BJ 9 showed reduced growth (0.195,0.585) after 24 and 72 hr. All the isolates showed optimum growth and at par with each other. Similar trend of growth was observed when these isolates were exposed to 0.75% concentration. Initially all isolates were quite sensitive at 0.8% concentration, while BJ 32 isolates showed minimum growth (0.166) followed by BJ 31 (0.171). Maximum growth was recorded in BJ 26 (0.255) followed by BJ 14 (0.248).

These weedicides have also tested through paper disc method using three concentrations . All the isolates showed good growth when they were exposed to three concentrations of persuit, kloben and pendamethalin.

Where all the isolates showed moderate sensitivity to persuit at all three concentration. While kloben and pendamethalin did not affect the growth and least inhibition was recorded in all the isolates .

Zawozink and Tomaro (2005) investigated the effect of chlorimuron ethyl on Bradyrhizobium japonicum and its symbiosis with soybean and reported that no change in nitrogen content, fresh biomass weight, leghaemoglobin content in nodules, nodule protein was not observed. Gonzalez, et al. (1999) reported that herbicides did not significantly affects the nodulation or nodule number in soybean. Ahemad and Khan (2011) also stated that the tolerance of glyphosate 3000 ug/ml against Rhizobium strains of chickpea, pea, green gram and lentil when amended in media. Soil microorganisms could use trifluralin at lower concentration as sole carbon and nitrogen for their growth, which indicated that microorganisms could decompose trifluralin . Adaptability of isolates in different areas might be due to existence of native isolates and genetic makeup of the particular isolates collected form specific environmental situation.

C. Compatibility of Bradyrhizobium isolates with insecticides Under crop protection the seeds are treated with insecticides to protect the seed from soil insects , hence three levels i.e. recommended, double and double and half than recommended rate of seed protecting insecticides were investigated against the growth of B.japonicum. Seed protectant insecticides thimathoxam had no detrimental effect on the bacterial population at all the test concentrations.

D. Compatibility with insecticides The maximum growth was recorded in BJ 12 (0.777) after 72 hr at 0.01%, where as BJ 16 showed (0.384) maximum growth at 24 hr. At 0.02% maximum growth i.e.0.266 and 0.643 was recorded in BJ 4 at 24 and 72 hr respectively where as minimum growth i.e.0.182, 0.487 was in BJ 29 and BJ 6 (0.182 and 0.472) at 24 and 72 hr respectively. At 0.04% some isolates exhibited slow or minimum growth at 24 hr. Minimum growth i.e.0.112 OD was recorded in BJ 27 followed by BJ 8 (0.114) where as BJ 3 gave maximum growth i.e. 0.281 followed

by BJ 12 (0.252), BJ 4 (0.244). Similar growth pattern was observed at 72 hr in all the isolates.

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V.V.Deshmukh et al., American International Journal of Research in Formal, Applied & Natural Sciences, 6(1), March-May 2014, pp. 5562

None of the isolates were found sensitive to imidacloprid when tested at three different levels. At 0.01% all isolates showed good growth. Imidacloprid supported the growth of BJ 3 (0.382) at 24 hr and 0.786 of BJ 2 at 72 hr respectively. At 0.02% BJ 31 showed maximum growth i.e.0.342 at 24 hr. Isolate BJ 11 showed maximum growth i.e. 0.734 at 72 hr .

At 0.04% isolate BJ 12 and BJ 11 showed maximum growth (0.292) after 24 hr, where as BJ 32 and BJ 21 showed minimum growth (0.172). BJ 18 showed minimum growth 0.278 at 72 hr.

Similarly the results of paper disc method revealed that all the isolates showed good compatibility with all test concentrations of the insecticide. No detrimental effect of both the insecticides was recorded in all isolates.

Singh,et al., (1991) studied the effect of carbosulfan, monocrotophos, methyl demeton, carbofuron and phorate on nodule number and weight of nodules of soybean. The nodule number 13.10 to 17.20 per plant were recorded over control 14.15 per plant. Carbofuron recorded significantly more nodule weight 100.85 mg/ plant followed by phorate. Ahemad and Khan (2011) recorded the tolerance of thiamethoxam and imidacloprid @ 2800 and 240 ug/ml and showed that Rhizobium could tolerated the toxicity of insecticides.

References Ahemad Munnes and Mohammad Sagir Khan 2011. Assessment of pesticidal tolerance and functional diversity of bacterial strains isolated from rhizosphere of different crops. Insight Microbiology .1(1):8-19.

Diatloff, A. 1986. Compatibility of systemic and non systemic fungicides with R.japonicum applied to soybean seed. Soil Biol.Biochem.

18(1): 121-122.

Gonzalez, A., C.Gonzalez Murua and M.Royuela.1996. Influence of imazethapyr on Rhizobium growth and its symbiosis with Pea (Pisum sativum) Weed Science. (44) 31-37.

Kaur Charanjit, P.Maini and N.P.Shukla 2007. Effect of captan and carbendazim fungicides on nodulation and Nitrogen fixation in Soybean. Assian J.Exp.Sci. 21(2): 385-388 .

Linda B. Lennox and Martin Alexander. 1981. Fungicide enhancement of nitrogen fixation and colonization of Phaseolus vulgaris by Rhizobium phaseoli. Appl. Environ. Microbiol. 42: 404-411.

Mallik M.A.B.and K.Tesfaic. 1985. Pesticidal effect on soybean rhizobia symbiosis. Plant and Soil 85: 33-41.

Muthamie J.W., P.E.Otieno, G.N. Cheminingwa, J.H. Nderitu and J.M.N. Wagacha. 2007 Effect of legume root rot pathogen and fungicides treatment on nodulation and biomass accumulation. J.Biol.Sci. ,7(7): 1163-1170.

Narayana Y.D.,D.Radhakrishna and P.V.Rai. 1984. Effect of certain fungicides on nodulation of cowpea (Vigna mungo). Pesticides. August 1984: 53-54.

Ndeye Fatou Diaw Guene, Adawa Diouf and Mamadou Gueye.2003. Nodulation and nitrogen fixation of field grown common bean (Phaseolus vulgaris) as influenced by fungicide seed treatment. African J. Biotechnol. 2(7): 198-121.

Singh Jagjeet and P.C.Lodha.1997.Compatibility of chemical seed protectants with Bradyrhizobium japonicum and their effect on symbiotic nodulation in soybean. J.Mycol.Pl.Pathol. 27(2): 148-151.

Singh T.,T.K.Ghosh,M.K.Tyagi and J.S.Duhan .1999. Fungicides and rhizobial interaction at different methods of inoculation in green gram. Legume Research. 22(3): 152-156.

Zawozink M.S. and Tomaro M.L. 2005. Effect of chlorimuron ethyl on Bradyrhizobium japonicum and its symbiosis. Pest Management Science. 61 (10): 1003-008.

Zilli Jerri Edson, Karen Gencolves Ribero, Ruben Jose Campo and Mariangela Hungria. 2009. Influence of fungicides seed treatment on soybean nodulation and grain yield. R.Bras.Ci.Solo ,33:917-923.

0.4 0.3 0.2 0.1 0

0.30% 0.45% BJ-32

BJ-31

BJ-30

BJ-29

BJ-28

BJ-27

BJ-26

BJ-25

BJ-24

BJ-23

BJ-22

BJ-21

BJ-20

BJ-19

BJ-18

BJ-17

BJ-16

BJ-15

BJ-14

BJ-13

BJ-12

BJ-11

BJ-9

BJ-10

BJ-8

BJ-7

BJ-6

BJ-5

BJ-4

BJ-3

BJ-2

0.60% BJ-1

Optical density

Compatibility of B.japonicum to different concentration of thiram at 24 hr.

0.75%

Isolates

0.8 0.6

0.30% 72 hr

0.4

0.45% 72 hr

0.2 BJ-32

BJ-31

BJ-30

BJ-29

BJ-28

BJ-27

BJ-26

BJ-25

BJ-24

BJ-23

BJ-22

BJ-21

BJ-20

BJ-19

BJ-18

BJ-17

BJ-16

BJ-15

BJ-14

BJ-13

BJ-12

BJ-11

BJ-10

BJ-9

BJ-8

BJ-7

BJ-6

BJ-5

BJ-4

BJ-3

0.60% 72 hr BJ-2

0

BJ-1

Optical density

Compatibility of B.japonicum to different concentration of thiram at 72 hr.

0.75% 72 hr

Isolates

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V.V.Deshmukh et al., American International Journal of Research in Formal, Applied & Natural Sciences, 6(1), March-May 2014, pp. 5562

Compatibility of B.japonicum to different concentration of carbendazim at 24 hr Optical density

0.4 0.3

0.10%

0.2

0.15%

0.1

0.20%

0

0.25%

Isolates

1 0.10% 72 hr

0.5

BJ-32

BJ-31

BJ-30

BJ-29

BJ-28

BJ-27

BJ-26

BJ-25

BJ-24

BJ-23

BJ-22

BJ-21

BJ-20

BJ-19

BJ-18

BJ-17

BJ-16

BJ-15

BJ-14

BJ-13

BJ-12

BJ-11

BJ-9

BJ-10

BJ-8

BJ-7

BJ-6

BJ-5

BJ-4

BJ-3

0.20% 72 hr BJ-2

0

0.15% 72 hr BJ-1

Optical density

Compatibility of B.japonicum to different concentration of carbendazim at 72 hr

0.25% 72 hr

Isolates

0.3 0.25 0.2 0.15 0.1 0.05 0

0.25% 0.37% 0.50% BJ-1 BJ-2 BJ-3 BJ-4 BJ-5 BJ-6 BJ-7 BJ-8 BJ-9 BJ-10 BJ-11 BJ-12 BJ-13 BJ-14 BJ-15 BJ-16 BJ-17 BJ-18 BJ-19 BJ-20 BJ-21 BJ-22 BJ-23 BJ-24 BJ-25 BJ-26 BJ-27 BJ-28 BJ-29 BJ-30 BJ-31 BJ-32

Optical density

Compatibility of B.japonicum to different concentration of COC at 24 hr

0.62%

Isolates

0.6 0.4

0.25%

0.2

0.37%

0

0.50% BJ-1 BJ-2 BJ-3 BJ-4 BJ-5 BJ-6 BJ-7 BJ-8 BJ-9 BJ-10 BJ-11 BJ-12 BJ-13 BJ-14 BJ-15 BJ-16 BJ-17 BJ-18 BJ-19 BJ-20 BJ-21 BJ-22 BJ-23 BJ-24 BJ-25 BJ-26 BJ-27 BJ-28 BJ-29 BJ-30 BJ-31 BJ-32

Optical density

Compatibility of B.japonicum to different concentration of COC at 72 hr

0.62%

Isolates

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V.V.Deshmukh et al., American International Journal of Research in Formal, Applied & Natural Sciences, 6(1), March-May 2014, pp. 5562

0.3 0.2

0.25%

0.1

0.37%

0

0.50% BJ-1 BJ-2 BJ-3 BJ-4 BJ-5 BJ-6 BJ-7 BJ-8 BJ-9 BJ-10 BJ-11 BJ-12 BJ-13 BJ-14 BJ-15 BJ-16 BJ-17 BJ-18 BJ-19 BJ-20 BJ-21 BJ-22 BJ-23 BJ-24 BJ-25 BJ-26 BJ-27 BJ-28 BJ-29 BJ-30 BJ-31 BJ-32

Optical density

Compatibility of B.japonicum to different concentration of mancozeb at 24 hr

0.62%

Isolates

Compatibility of B.japonicum to different concentration of mancozeb at 72 hr Optical density

0.8 0.6 0.25%

0.4

0.37%

0.2

0.50% BJ-1 BJ-2 BJ-3 BJ-4 BJ-5 BJ-6 BJ-7 BJ-8 BJ-9 BJ-10 BJ-11 BJ-12 BJ-13 BJ-14 BJ-15 BJ-16 BJ-17 BJ-18 BJ-19 BJ-20 BJ-21 BJ-22 BJ-23 BJ-24 BJ-25 BJ-26 BJ-27 BJ-28 BJ-29 BJ-30 BJ-31 BJ-32

0

0.62%

Isolates

Compatibility of B.japonicum to different concentration of ridomil at 24 hr

0.4

BJ-32

BJ-31

BJ-30

BJ-29

BJ-28

BJ-27

BJ-26

BJ-25

BJ-24

BJ-23

BJ-22

BJ-21

BJ-20

BJ-19

BJ-18

BJ-17

BJ-16

BJ-15

BJ-14

BJ-13

BJ-12

BJ-11

BJ-9

BJ-10

0.50% BJ-8

0

BJ-7

0.40% BJ-6

0.1 BJ-5

0.30%

BJ-4

0.2

BJ-3

0.20%

BJ-2

0.3

BJ-1

Optical density

0.5

Isolates

Compatibility of B.japonicum to different concentration of ridomil at 72 hr Optical density

0.8 0.6 0.20%

0.4

0.30%

0.2

0.40% BJ-1 BJ-2 BJ-3 BJ-4 BJ-5 BJ-6 BJ-7 BJ-8 BJ-9 BJ-10 BJ-11 BJ-12 BJ-13 BJ-14 BJ-15 BJ-16 BJ-17 BJ-18 BJ-19 BJ-20 BJ-21 BJ-22 BJ-23 BJ-24 BJ-25 BJ-26 BJ-27 BJ-28 BJ-29 BJ-30 BJ-31

0

0.50%

Isolates

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V.V.Deshmukh et al., American International Journal of Research in Formal, Applied & Natural Sciences, 6(1), March-May 2014, pp. 5562

Table 1: Compatibility of Bradyrhiziobium japonicum with fungicides (at 72 hr) (Spectrophotometrically) Isolates

Thiram Carbendazim 0.30% 0.45% 0.60% 0.75% 0.10% 0.15% 0.573 0.480 0.333 0.170 0.660 0.622 BJ-1 0.535 0.494 0.312 0.148 0.734 0.654 BJ-2 0.605 0.517 0.289 0.126 0.652 0.701 BJ-3 0.635 0.489 0.303 0.139 0.685 0.681 BJ-4 0.546 0.525 0.296 0.130 0.681 0.718 BJ-5 0.594 0.485 0.291 0.126 0.711 0.668 BJ-6 0.566 0.524 0.316 0.162 0.719 0.707 BJ-7 0.525 0.497 0.329 0.165 0.780 0.663 BJ-8 0.516 0.484 0.300 0.137 0.725 0.641 BJ-9 BJ-10 0.612 0.526 0.328 0.164 0.683 0.711 BJ-11 0.617 0.515 0.321 0.157 0.662 0.621 BJ-12 0.622 0.483 0.314 0.152 0.728 0.658 BJ-13 0.600 0.514 0.297 0.133 0.719 0.685 BJ-14 0.476 0.494 0.319 0.156 0.691 0.760 BJ-15 0.547 0.535 0.310 0.148 0.654 0.696 BJ-16 0.571 0.519 0.288 0.125 0.694 0.713 BJ-17 0.541 0.522 0.314 0.148 0.718 0.753 BJ-18 0.513 0.455 0.283 0.118 0.672 0.745 BJ-19 0.605 0.497 0.295 0.131 0.721 0.705 BJ-20 0.565 0.481 0.304 0.141 0.626 0.709 BJ-21 0.510 0.530 0.303 0.137 0.714 0.720 BJ-22 0.616 0.470 0.323 0.157 0.710 0.633 BJ-23 0.534 0.525 0.302 0.139 0.722 0.713 BJ-24 0.555 0.519 0.314 0.150 0.693 0.616 BJ-25 0.627 0.519 0.322 0.159 0.713 0.643 BJ-26 0.581 0.505 0.297 0.131 0.754 0.694 BJ-27 0.598 0.514 0.333 0.168 0.658 0.744 BJ-28 0.598 0.475 0.314 0.152 0.658 0.630 BJ-29 0.587 0.500 0.327 0.165 0.666 0.731 BJ-30 0.523 0.498 0.283 0.119 0.738 0.714 BJ-31 0.553 0.527 0.307 0.142 0.694 0.619 BJ-32 0.643 0.516 0.309 0.145 0.727 0.656 0.06 0.04 0.02 0.01 0.16 SE(M)+ 0.02 CD 0.18 0.1 0.07 0.04 0.43 p=0.01 0.06 Average 0.571 0.0504 0.309 0.145 0.699+ 0.685+ SD + +0.042 +0.020 +0.014 +0.014 0.068 0.086

0.20% 0.471 0.497 0.518 0.506 0.567 0.515 0.556 0.510 0.486 0.557 0.466 0.510 0.534 0.526 0.539 0.559 0.603 0.591 0.552 0.553 0.558 0.479 0.466 0.468 0.489 0.542 0.588 0.476 0.575 0.552 0.559 0.527 0.06

0.25% 0.421 0.450 0.505 0.486 0.516 0.504 0.515 0.463 0.432 0.543 0.440 0.453 0.510 0.478 0.497 0.518 0.544 0.450 0.590 0.425 0.454 0.410 0.415 0.512 0.480 0.524 0.425 0.484 0.495 0.555 0.467 0.420 0.83

Copper oxychloride 0.25% 0.37% 0.50% 0.62% 0.450 0.485 0.438 0.384 0.492 0.518 0.465 0.408 0.470 0.539 0.492 0.438 0.481 0.511 0.458 0.404 0.488 0.488 0.445 0.390 0.472 0.543 0.495 0.441 0.463 0.552 0.508 0.454 0.451 0.519 0.476 0.425 0.468 0.512 0.466 0.412 0.483 0.521 0.472 0.418 0.460 0.513 0.474 0.427 0.481 0.549 0.503 0.449 0.486 0.498 0.444 0.390 0.464 0.512 0.467 0.413 0.455 0.531 0.466 0.392 0.488 0.481 0.436 0.352 0.457 0.559 0.516 0.462 0.453 0.556 0.516 0.423 0.466 0.548 0.498 0.444 0.479 0.552 0.504 0.450 0.442 0.459 0.416 0.362 0.465 0.512 0.458 0.403 0.445 0.484 0.438 0.384 0.462 0.502 0.460 0.410 0.474 0.508 0.462 0.408 0.475 0.545 0.499 0.451 0.490 0.523 0.475 0.421 0.482 0.510 0.462 0.438 0.484 0.524 0.473 0.419 0.483 0.532 0.482 0.428 0.454 0.552 0.505 0.451 0.481 0.536 0.488 0.427 0.02 0.04 0.02 0.03

Mancozeb Ridomil 0.25% 0.37% 0.50% 0.62% 0.20% 0.30% 0.40% 0.50% 0.578 0.449 0.459 0.369 0.669 0.529 0.479 0.419 0.597 0.470 0.478 0.388 0.688 0.548 0.498 0.438 0.613 0.484 0.494 0.404 0.704 0.564 0.514 0.454 0.632 0.503 0.513 0.423 0.723 0.583 0.533 0.473 0.590 0.461 0.471 0.381 0.681 0.541 0.491 0.431 0.574 0.445 0.455 0.365 0.665 0.525 0.475 0.415 0.643 0.514 0.524 0.434 0.734 0.594 0.544 0.484 0.620 0.491 0.501 0.411 0.711 0.571 0.521 0.461 0.598 0.469 0.479 0.389 0.689 0.549 0.499 0.439 0.633 0.504 0.514 0.424 0.724 0.584 0.534 0.474 0.633 0.504 0.514 0.424 0.724 0.584 0.534 0.474 0.601 0.472 0.482 0.392 0.692 0.552 0.502 0.442 0.631 0.502 0.512 0.422 0.722 0.582 0.532 0.472 0.614 0.485 0.495 0.405 0.705 0.565 0.515 0.455 0.611 0.482 0.492 0.402 0.702 0.562 0.512 0.452 0.634 0.505 0.515 0.424 0.725 0.585 0.535 0.475 0.635 0.506 0.516 0.426 0.726 0.586 0.536 0.476 0.584 0.455 0.465 0.375 0.675 0.535 0.485 0.425 0.609 0.480 0.490 0.400 0.700 0.560 0.510 0.450 0.626 0.497 0.507 0.417 0.717 0.577 0.527 0.467 0.601 0.472 0.482 0.392 0.692 0.552 0.502 0.442 0.615 0.486 0.496 0.406 0.706 0.566 0.516 0.456 0.611 0.482 0.492 0.402 0.702 0.562 0.512 0.452 0.633 0.504 0.514 0.424 0.724 0.584 0.534 0.474 0.640 0.511 0.521 0.431 0.731 0.591 0.541 0.481 0.639 0.510 0.520 0.430 0.730 0.590 0.540 0.480 0.630 0.501 0.511 0.421 0.721 0.581 0.531 0.471 0.629 0.500 0.509 0.420 0.720 0.580 0.530 0.470 0.596 0.467 0.477 0.387 0.687 0.547 0.497 0.437 0.607 0.478 0.488 0.398 0.698 0.558 0.508 0.448 0.602 0.473 0.483 0.393 0.693 0.553 0.503 0.443 0.593 0.464 0.474 0.384 0.684 0.544 0.494 0.434 0.02 0.03 0.03 0.03 0.02 0.04 0. 04 0.04

0.16 0.16 0.04 0.12 0.06 0.09 0.07 0.09 0.09 0.09 0.06 0.1 0.11 0.528+ 0.481+ 0.470 0.521 0.474 0.418 0.614 0.485 0.495 0.405 0.689 0.565 0.515 .079 0.092 +0.142 +0.252 +0.025 +0.027 +0.019 +0.019 +0.019 +0.019 +0.033 +0.019 +0.019

0.12 0.455 +0.02

Table 2: Compatibility of Bradyrhiziobium japonicum with fungicides (at 24 hr) (Spectrophotometrically) Isolates Thiram

Carbendazim

Copper oxychloride

Mancozeb

Ridomil

BJ-1

0.30% 0.45% 0.60% 0.75% 0.10% 0.15% 0.20% 0.25% 0.25% 0.37% 0.50% 0.62% 0.25% 0.37% 0.50% 0.62% 0.20% 0.30% 0.40% 0.50% 0.251 0.205 0.173 0.088 0.212 0.218 0.171 0.121 0.183 0.184 0.154 0.146 0.189 0.169 0.159 0.149 0.279 0.319 0.259 0.239

BJ-2 BJ-3

0.213 0.283

0.219 0.242

0.151 0.129

0.066 0.044

0.281 0.219

0.232 0.252

0.197 0.248

0.149 0.195

0.223 0.189

0.211 0.238

0.181 0.208

0.155 0.164

0.208 0.224

0.188 0.204

0.178 0.194

0.168 0.184

0.298 0.314

0.338 0.354

0.278 0.294

0.258 0.274

BJ-4 BJ-5 BJ-6 BJ-7

0.312 0.224 0.272 0.244

0.214 0.250 0.210 0.250

0.142 0.133 0.130 0.165

0.057 0.048 0.045 0.080

0.255 0.243 0.270 0.267

0.276 0.273 0.252 0.263

0.206 0.267 0.215 0.257

0.154 0.217 0.290 0.211

0.212 0.224 0.204 0.194

0.204 0.191 0.241 0.254

0.174 0.161 0.211 0.224

0.153 0.148 0.165 0.169

0.243 0.201 0.185 0.254

0.223 0.181 0.165 0.234

0.213 0.171 0.155 0.224

0.203 0.161 0.145 0.214

0.333 0.291 0.275 0.344

0.373 0.331 0.315 0.384

0.313 0.271 0.255 0.324

0.293 0.251 0.235 0.304

BJ-8 BJ-9

0.203 0.194

0.222 0.209

0.168 0.140

0.083 0.055

0.324 0.280

0.233 0.227

0.209 0.186

0.163 0.134

0.181 0.203

0.222 0.212

0.192 0.182

0.159 0.155

0.231 0.209

0.211 0.189

0.201 0.179

0.191 0.169

0.321 0.299

0.361 0.339

0.301 0.279

0.281 0.259

BJ-10 0.290 BJ-11 0.295

0.251 0.240

0.167 0.160

0.082 0.075

0.241 0.232

0.266 0.231

0.257 0.166

0.245 0.119

0.214 0.196

0.222 0.220

0.188 0.190

0.157 0.158

0.244 0.244

0.224 0.224

0.214 0.214

0.204 0.204

0.334 0.334

0.374 0.374

0.314 0.314

0.294 0.294

0.300 0.278 0.154 0.225

0.208 0.239 0.219 0.230

0.155 0.136 0.159 0.151

0.070 0.051 0.074 0.066

0.275 0.269 0.252 0.221

0.245 0.252 0.248 0.243

0.210 0.234 0.226 0.239

0.154 0.184 0.173 0.195

0.210 0.213 0.194 0.180

0.249 0.190 0.213 0.212

0.219 0.160 0.183 0.182

0.168 0.148 0.156 0.155

0.212 0.242 0.225 0.222

0.192 0.222 0.205 0.202

0.182 0.212 0.195 0.192

0.172 0.202 0.185 0.182

0.302 0.332 0.315 0.312

0.342 0.372 0.355 0.352

0.282 0.312 0.295 0.292

0.262 0.292 0.275 0.272

BJ-16 0.249

0.244

0.127

0.042

0.252

0.266

0.259

0.210

0.220

0.182

0.152

0.145

0.245

0.225

0.215

0.204

0.335

0.375

0.315

0.295

BJ-17 0.219 BJ-18 0.191

0.247 0.180

0.151 0.121

0.066 0.036

0.253 0.239

0.257 0.265

0.303 0.291

0.241 0.183

0.187 0.191

0.262 0.262

0.232 0.211

0.172 0.172

0.246 0.195

0.226 0.175

0.216 0.165

0.206 0.155

0.336 0.285

0.376 0.325

0.316 0.265

0.296 0.245

0.282 0.243 0.188 0.294 0.212

0.222 0.206 0.255 0.195 0.250

0.134 0.144 0.141 0.160 0.142

0.049 0.059 0.056 0.075 0.057

0.281 0.208 0.273 0.279 0.279

0.255 0.261 0.268 0.231 0.223

0.252 0.253 0.258 0.179 0.166

0.205 0.202 0.215 0.126 0.119

0.199 0.214 0.174 0.194 0.181

0.244 0.250 0.162 0.204 0.184

0.214 0.220 0.132 0.174 0.154

0.166 0.168 0.139 0.153 0.146

0.220 0.237 0.212 0.226 0.222

0.200 0.217 0.192 0.206 0.202

0.190 0.207 0.182 0.196 0.192

0.180 0.197 0.172 0.186 0.182

0.310 0.327 0.302 0.316 0.312

0.350 0.367 0.342 0.356 0.352

0.290 0.307 0.282 0.296 0.292

0.270 0.287 0.262 0.276 0.272

BJ-24 0.233 BJ-25 0.305

0.244 0.244

0.153 0.162

0.068 0.077

0.212 0.273

0.214 0.225

0.158 0.189

0.109 0.140

0.191 0.210

0.210 0.208

0.177 0.178

0.153 0.154

0.244 0.251

0.224 0.231

0.214 0.221

0.204 0.211

0.334 0.341

0.374 0.381

0.314 0.321

0.294 0.301

BJ-26 0.259 BJ-27 0.276

0.230 0.239

0.134 0.171

0.049 0.086

0.284 0.218

0.232 0.273

0.242 0.288

0.177 0.227

0.200 0.222

0.245 0.221

0.215 0.191

0.166 0.158

0.250 0.241

0.230 0.221

0.220 0.211

0.210 0.201

0.340 0.331

0.380 0.371

0.320 0.311

0.300 0.291

BJ-28 0.276 BJ-29 0.265

0.200 0.225

0.155 0.168

0.070 0.083

0.228 0.235

0.225 0.263

0.176 0.275

0.122 0.187

0.214 0.222

0.208 0.219

0.178 0.189

0.154 0.158

0.240 0.207

0.220 0.187

0.210 0.177

0.200 0.167

0.330 0.297

0.370 0.337

0.310 0.277

0.290 0.257

BJ-30 0.201 BJ-31 0.231

0.223 0.252

0.122 0.145

0.037 0.060

0.287 0.253

0.247 0.232

0.262 0.259

0.195 0.255

0.213 0.185

0.228 0.251

0.198 0.221

0.161 0.168

0.218 0.213

0.198 0.193

0.188 0.183

0.178 0.173

0.308 0.303

0.348 0.343

0.288 0.283

0.268 0.263

BJ-32 0.321

0.241

0.148

0.063

0.274

0.225

0.227

0.171

0.206

0.234

0.204

0.163

0.204

0.184

0.174

0.164

0.294

0.334

0.274

0.254

0.04

0.03

0.01

0.08

0.03

0.01

0.01

0.02

0.02

0.04

0.04

0.02

0.04

0.04

0.04

0.02

0.04

0.02

0.03

0.12

0.09

0.04

0.21

0.1

0.04

0.41

0.05

0.05

0.1

0.1

0.06

0.1

0.12

0.11

0.056

0.11

0.06

0.08

BJ-12 BJ-13 BJ-14 BJ-15

BJ-19 BJ-20 BJ-21 BJ-22 BJ-23

SE(M)+ 0.03 CD p=0.01 0.09

0.220 .0189 0.158 0.225 0.205 0.195 0.185 0.315 0.355 0.295 0.275 Average 0.249 0.228 0.148 0.063 0.255+ 0.246+ 0.229+ 0.181+ 0.201 SD + +0.042 +0.019 +0.014 +0.014 0.055 0.037 0.081 0.090 +0.0146 +0.025 +0.245 +0.008 +0.019 +0.019 +0.019 +0.019 +0.019 +0.019 +0.019 +0.019

AIJRFANS 14-228; Š 2014, AIJRFANS All Rights Reserved

Page 61

V.V.Deshmukh et al., American International Journal of Research in Formal, Applied & Natural Sciences, 6(1), March-May 2014, pp. 5562

Table 3: Compatibility of Bradyrhiziobium japonicum with herbicides (Spectrophotometrically) Isolates

Persuit 0.25%

0.15%

0.30%

Kloben 0.12%

0.08%

0.20%

Pendamethalin 0.75%

0.66%

24 hr 0.208

0.80%

BJ-1

24hr 0.157

72hr 0.444

24 hr 0.166

72 hr 0.464

24 hr 0.173

72 hr 0.431

24 hr 0.207

72 hr 0.407

24 hr 72 hr 24 hr 72 hr 24 hr 72 hr 0.257 0.404 0.127 0.217 0.210 0.600

BJ-2 BJ-3 BJ-4 BJ-5 BJ-6 BJ-7 BJ-8 BJ-9 BJ-10 BJ-11 BJ-12 BJ-13 BJ-14 BJ-15 BJ-16 BJ-17 BJ-18 BJ-19 BJ-20 BJ-21 BJ-22 BJ-23 BJ-24

0.191 0.133 0.187 0.154 0.103 0.149 0.148 0.176 0.141 0.198 0.202 0.170 0.165 0.173 0.144 0.145 0.113 0.153 0.167 0.134 0.191 0.180 0.154

0.478 0.420 0.474 0.441 0.390 0.436 0.435 0.463 0.428 0.485 0.489 0.457 0.452 0.460 0.431 0.432 0.400 0.440 0.454 0.421 0.478 0.467 0.441

0.179 0.132 0.145 0.167 0.120 0.100 0.137 0.163 0.184 0.143 0.180 0.145 0.175 0.163 0.185 0.145 0.131 0.150 0.178 0.163 0.178 0.186 0.133

0.477 0.430 0.444 0.465 0.419 0.398 0.432 0.461 0.482 0.441 0.478 0.443 0.473 0.462 0.484 0.444 0.429 0.449 0.476 0.462 0.476 0.484 0.432

0.266 0.233 0.269 0.258 0.104 0.257 0.218 0.176 0.182 0.277 0.275 0.239 0.257 0.245 0.219 0.221 0.109 0.215 0.234 0.201 0.190 0.192 0.212

0.415 0.409 0.428 0.421 0.305 0.434 0.419 0.405 0.428 0.454 0.432 0.424 0.425 0.442 0.438 0.428 0.314 0.418 0.429 0.415 0.418 0.423 0.426

0.241 0.195 0.237 0.213 0.153 0.199 0.198 0.226 0.191 0.248 0.252 0.220 0.215 0.222 0.194 0.195 0.163 0.203 0.217 0.184 0.241 0.230 0.204

0.441 0.428 0.437 0.413 0.353 0.399 0.400 0.426 0.391 0.448 0.452 0.420 0.415 0.422 0.394 0.395 0.363 0.403 0.417 0.384 0.441 0.438 0.404

0.291 0.245 0.287 0.263 0.203 0.249 0.248 0.276 0.241 0.298 0.302 0.270 0.266 0.272 0.244 0.245 0.213 0.253 0.267 0.234 0.291 0.280 0.254

0.437 0.392 0.434 0.409 0.350 0.396 0.395 0.423 0.388 0.445 0.452 0.417 0.411 0.418 0.390 0.392 0.360 0.400 0.414 0.381 0.438 0.427 0.400

0.161 0.115 0.157 0.129 0.073 0.119 0.118 0.146 0.111 0.168 0.172 0.140 0.136 0.142 0.114 0.115 0.083 0.123 0.137 0.104 0.161 0.150 0.124

0.251 0.205 0.247 0.223 0.163 0.209 0.208 0.236 0.201 0.258 0.262 0.230 0.225 0.232 0.204 0.205 0.173 0.213 0.227 0.194 0.251 0.240 0.213

0.274 0.254 0.274 0.298 0.203 0.270 0.253 0.195 0.242 0.280 0.264 0.220 0.280 0.224 0.219 0.247 0.264 0.217 0.227 0.245 0.254 0.217 0.262

0.664 0.644 0.654 0.688 0.593 0.660 0.643 0.585 0.632 0.670 0.654 0.610 0.670 0.614 0.609 0.637 0.654 0.607 0.617 0.635 0.641 0.607 0.652

0.272 0.252 0.272 0.296 0.201 0.268 0.253 0.193 0.240 0.278 0.262 0.218 0.278 0.222 0.217 0.245 0.262 0.215 0.225 0.243 0.253 0.215 0.260

0.598 0.578 0.598 0.623 0.506 0.594 0.576 0.519 0.566 0.604 0.588 0.542 0.604 0.548 0.543 0.571 0.588 0.531 0.551 0.569 0.579 0.533 0.586

0.242 0.222 0.242 0.266 0.171 0.238 0.223 0.163 0.210 0.248 0.232 0.188 0.248 0.192 0.187 0.215 0.232 0.185 0.195 0.213 0.223 0.185 0.230

0.623 0.603 0.622 0.646 0.551 0.618 0.603 0.543 0.590 0.628 0.612 0.568 0.628 0.572 0.567 0.595 0.612 0.565 0.575 0.593 0.603 0.565 0.610

BJ-25 BJ-26 BJ-27 BJ-28 BJ-29 BJ-30 BJ-31 BJ-32 SE(M)+ CD p=0.01

0.188 0.180 0.138 0.149 0.120 0.136 0.133 0.122 0.028

0.475 0.467 0.425 0.436 0.407 0.423 0.420 0.409 0.013

0.156 0.170 0.178 0.186 0.110 0.107 0.158 0.150 0.035

0.455 0.469 0.476 0.485 0.409 0.405 0.457 0.448 0.041

0.221 0.257 0.210 0.235 0.164 0.173 0.250 0.114 0.042

0.428 0.434 0.415 0.426 0.402 0.402 0.437 0.322 0.036

0.238 0.230 0.188 0.199 0.170 0.186 0.183 0.172 0.03

0.430 0.430 0.384 0.399 0.346 0.386 0.383 0.372 0.069

0.288 0.280 0.238 0.249 0.221 0.236 0.233 0.222 0.039

0.435 0.427 0.385 0.395 0.343 0.382 0.380 0.368 0.062

0.158 0.150 0.108 0.119 0.090 0.106 0.103 0.092 0.042

0.248 0.240 0.198 0.209 0.180 0.196 0.193 0.182 0.032

0.246 0.287 0.238 0.203 0.231 0.258 0.203 0.198 0.044

0.636 0.677 0.628 0.593 0.621 0.648 0.593 0.622 0.061

0.244 0.285 0.236 0.201 0.229 0.256 0.201 0.196 0.04

0.570 0.611 0.562 0.518 0.555 0.582 0.527 0.547 0.05

0.214 0.255 0.206 0.171 0.199 0.226 0.171 0.166 0.03

0.594 0.635 0.586 0.551 0.579 0.606 0.551 0.546 0.032

0.079

0.037

0.097

0.114

0.115

0.099

0.084

0.192

0.1

0.14

Average SD

0.156 +0.025

0.443 +0.025

0.155 +0.024

0.453 +0.022

0.231 +0.046

0.413 +0.034

0.206 +0.025

0.107 0.172 0.118 0.09 0.123 0.169 0.402 0.126 0.216 0.242 0.406 0.256 +0.02 +0.02 +0.02 +0.02 0.633 +0.027 +0.025 7 5 5 8 +0.027

0.240 +0.028

72 hr 24 hr 72 hr 0.534 0.178 0.558

0.09 0.089 0.585 0.563 0.208 +0.03 +0.030 +0.029 0

Table 4: Compatibility of Bradyrhiziobium japonicum with insecticides (Spectrophotometrically) Thiamethoxam Isolates

0.01%

Imidacloprid

0.02%

0.04%

0.01%

0.02%

0.04%

24 hr

72 hr

24 hr

72 hr

24 hr

72 hr

24hr

72 hr

24 hr

72 hr

24 hr

72 hr

BJ-1 BJ-2 BJ-3 BJ-4

0.290 0.342 0.250 0.352

0.653 0.751 0.582 0.754

0.221 0.242 0.241 0.266

0.632 0.562 0.611 0.574

0.211 0.232 0.281 0.244

0.532 0.551 0.612 0.643

0.243 0.262 0.210 0.272

0.653 0.661 0.492 0.664

0.243 0.312 0.260 0.325

0.633 0.691 0.542 0.714

0.376 0.382 0.313 0.369

0.744 0.786 0.680 0.752

BJ-5 BJ-6 BJ-7

0.313 0.222 0.343

0.652 0.512 0.723

0.262 0.182 0.235

0.513 0.490 0.534

0.239 0.184 0.244

0.556 0.472 0.541

0.233 0.182 0.263

0.562 0.422 0.633

0.283 0.232 0.313

0.612 0.472 0.683

0.333 0.302 0.379

0.691 0.582 0.782

BJ-8 BJ-9 BJ-10 BJ-11 BJ-12 BJ-13 BJ-14 BJ-15 BJ-16 BJ-17 BJ-18 BJ-19 BJ-20 BJ-21 BJ-22 BJ-23 BJ-24

0.301 0.257 0.232 0.372 0.372 0.232 0.323 0.281 0.384 0.312 0.213 0.233 0.304 0.212 0.310 0.285 0.311

0.616 0.660 0.561 0.774 0.777 0.585 0.721 0.611 0.697 0.668 0.485 0.713 0.743 0.541 0.642 0.625 0.633

0.246 0.233 0.250 0.246 0.252 0.243 0.243 0.235 0.253 0.250 0.182 0.247 0.261 0.195 0.234 0.220 0.232

0.505 0.612 0.553 0.582 0.532 0.512 0.542 0.581 0.573 0.511 0.469 0.633 0.499 0.513 0.564 0.512 0.472

0.114 0.241 0.223 0.252 0.202 0.172 0.212 0.251 0.162 0.223 0.183 0.204 0.143 0.183 0.202 0.181 0.142

0.562 0.585 0.582 0.571 0.632 0.567 0.565 0.564 0.551 0.582 0.487 0.623 0.603 0.520 0.573 0.554 0.512

0.262 0.255 0.192 0.292 0.292 0.275 0.243 0.230 0.241 0.232 0.173 0.280 0.291 0.172 0.254 0.233 0.231

0.596 0.664 0.471 0.684 0.687 0.664 0.631 0.521 0.651 0.578 0.395 0.679 0.667 0.451 0.615 0.627 0.543

0.276 0.314 0.242 0.342 0.342 0.269 0.293 0.280 0.334 0.282 0.223 0.333 0.308 0.222 0.289 0.255 0.281

0.575 0.646 0.521 0.734 0.700 0.667 0.681 0.571 0.606 0.628 0.445 0.691 0.677 0.501 0.661 0.655 0.593

0.348 0.354 0.314 0.321 0.369 0.381 0.352 0.374 0.366 0.340 0.309 0.350 0.369 0.301 0.363 0.371 0.310

0.745 0.754 0.594 0.601 0.752 0.737 0.744 0.760 0.783 0.760 0.564 0.749 0.769 0.581 0.762 0.771 0.590

BJ-25 BJ-26 BJ-27 BJ-28 BJ-29 BJ-30 BJ-31 BJ-32 SE(M)+ CD p=0.01 Average SD

0.295 0.351 0.222 0.310 0.202 0.242 0.366 0.212 0.02

0.634 0.762 0.596 0.764 0.531 0.571 0.773 0.543 0.01

0.243 0.235 0.232 0.245 0.182 0.202 0.254 0.224 0.03

0.582 0.563 0.486 0.508 0.514 0.531 0.533 0.521 0.027

0.192 0.182 0.112 0.151 0.184 0.201 0.161 0.191 0.06

0.567 0.542 0.553 0.644 0.487 0.564 0.582 0.563 0.033

0.250 0.271 0.214 0.264 0.162 0.162 0.246 0.172 0.037

0.633 0.651 0.591 0.692 0.441 0.481 0.655 0.453 0.05

0.302 0.352 0.263 0.325 0.212 0.212 0.342 0.222 0.03

0.682 0.682 0.633 0.654 0.491 0.531 0.672 0.503 0.02

0.340 0.365 0.354 0.379 0.304 0.355 0.383 0.313 0.04

0.760 0.794 0.716 0.782 0.584 0.743 0.784 0.593 0.015

0.07 0.288 +0.055

0.04 0.651 +0.086

0.09 0.234 +0.022

0.074 0.541 +0.044

0.18 0.196 +0.040

0.091 0.563 +0.041

0.103 0.236 + 0.039

0.13 0.587 + 0.091

0.09 0.283 + 0.042

0.05 0.617 + 0.078

0.11 0.348 + 0.027

0.043 0.712 + 0.078

AIJRFANS 14-228; Š 2014, AIJRFANS All Rights Reserved

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