BLUE BOTTLE EXPERIMENT
CHEMISTRY DEMONSTRATION EXPERIMENT IN ORGANIC CHEMISTRY
DEMONSTRATION EXPERIMENT IN SECONDARY STAGE II
BLUE BOTTLE EXPERIMENT
524 013
Sensor-CASSY 2
EXPERIMENT C2.2.2.2
524 220
CASSY Lab 2
The blue bottle experiment is a favourite demonstration experiment in practical lectures. In the classic version, the experimenter shakes a bottle filled with a clear liquid which then turns blue. After a while the liquid becomes colourless, but then turns blue again through shaking. The experiment is based on the redox indicator, methylene blue. Methylene blue is blue in its oxidised form, and in its reduced form, the so-called leucomethylene blue, it's colourless.
CONTENTS OF THE BLUE BOTTLE EXPERIMENT 524 013
Sensor-CASSY 2
524 220
CASSY Lab 2
524 069
Immersion photometer S
666 425
Profile frame C50, two rows, for CPS
666 438
Woulff‘s bottle with manometer, CPS
667 3095
Screw cap, GL 45, mB
667 3107
Silicone gasket, GL 45/26, set of 10
666 482
Aeration pump, controllable, CPS
501 44
Connecting leads 19 A, 25 cm, red/blue, pair
667 7977
Compact balance 440-3N, 200 g: 0.01 g
664 157
Watch glass dish, 125 mm diam.
666 966
Spoon-ended spatula, SS, 180 mm
666 003
Pipetting ball (Peleus ball)
665 996
Graduated pipette 5 ml
665 756
Measuring cylinder 500 ml with plastic base
604 501
PVC tubing 7 mm diam., 1 m
604 460
Hose clamp 8...12 mm
602 347
Laboratory bottle to DIN standard, 500 ml , GL 45
673 2920
Löffler's methylene blue solution, 100 ml
673 6800
Sodium hydroxide pellets, 100 g
672 1100
D(+)-Glucose, 100 g
675 3400
Water, pure, 1 L additionally required: PC with Windows XP/Vista/7/8 Blue bottle experiment
Our version is a modification of the classic blue bottle experiment. Instead of shaking, the air is pumped through the reaction mixture. This takes place automatically. The transmission of the solution is continually measured using an immersion photometer. As soon as the solution has become colourless, air is pumped through until the solution is coloured deep blue again. The pump then switches off automatically. The solution slowly loses its colour and the process then begins anew.
TOPICS The use of carbohydrates such as glucose as reducing agents Performing the blue bottle experiment in a new, automated version
Setting up and using a two-point control system Photometry using an immersion photometer Learning about organic redox reactions and the redox indicator methylene blue
WWW.LEYBOLD-SHOP.COM BRANDS OF THE LD DIDACTIC GROUP
1100602EN
C2.2.2.2
Here, the reducing sugar glucose acts as the reducing agent. It reduces methylene blue to colourless leucomethylene blue. By shaking, oxygen diffuses through the solution and re-oxidises the leucomethylene blue to methylene blue. This is then reduced again by the glucose. The reaction continues to run until there is no more oxygen available in the air or the glucose reducing agent has been depleted.