Digital Microscopy Image Analysis

Digital Image Analysis _Maria Li

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1mm Original image of crayfish’s compound eye (800x) 5

1 Volume viewer 2 Zoom in 3 LUT (Phase) - to highlight the surface pattern 4 Enhance contrast - highlight facets zoom in 5 3D Surface plot (Mesh) 6 3D Surface plot (Thermal) 7 3D Surface plot (Gradient) 8 3D Surface plot (Isoline)

UNIT 1: Dissecting Biological Machines

_Maria Li

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Original image of crayfish’s gill fillaments (40x)

1 Probability map 2 Binary outline 3 Trainable weka segmentation - to classify different elements 4 3D surface plot of classified segmentation product 5 Color surface 3D viewer 6 LUT (ICA) - to highlight the outline of setobranches 7 Binary of setobranches zoom in 8 3D surface plot of setobranches

UNIT 1: Dissecting Biological Machines

_Maria Li

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Original image of crayfish’s muscle cells(100x)

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1 LUT (Phase) - to color coded red pigment and muscle fiber 2 LUT (3-3-2-RGB) - to hightlight the surface texture 3 Integrated image filter (Sample variance) - to highlight the outlines of the pigment 4 Watershed pattern of pigment 5 Zoom in linear muscle cells 6 3D surface plot (Fill) - to create 3D texture of muscle fiber 7 LUT (Physic) of linear muscle cells zoom in 8 3D Surface plot (Mesh) - to create 3D texture of muscle fiber

UNIT 1: Dissecting Biological Machines

_Maria Li

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Original image of lichen(100x)

1 Sample variance 2 Subtract background 3 LUT (Phase) 4 LUT (3-3-2 RGB) 5 3D Surface plot (Mesh) of fruit body 6 3D Surface plot (Dot)of fruit body 7 3D Surface plot (Isoline) of fruit body 8 3D Surface plot (Filled) of fruit body

UNIT 1: Dissecting Biological Machines

_Maria Li

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Original image of moth wing(100x)

1 LUT (fire) - to highlight the pattern of the scales 2 Subtract background 3 Binnary outline 4 LUT (3-3-2 RGB) 5 Find edges of wing scale zoom in 6 3D surface plot (filled) - to illustate the 3D texture of wing scale patter 7 Greyscale of wing edge zoom in 8 3D surface plot (filleled) - to illustrate patterns of wing edge and scales

UNIT 1: Dissecting Biological Machines

_Maria Li

UAL Central Saint Martins MA Biodesign Unit 1: Disecting Biological Machine Course Leader:Nancy Diniz Lecturer in Biology and Living Systems:Alice Taylor Grow Lab Technician:Shem Johnson Student: Maria Li IG: @mariali_biotinker