An introduction to the use of molecular genetics in plant breeding

Page 1

Contact: Dr. Naglaa A. Ashry

naglaashry@yahoo.com


An introduction to the use of molecular genetics in plant breeding 1. Native PAGE 2. Native Gradient PAGE 3. Urea PAGE

4. SDS PAGE 5. SDS Gradient PAGE

6. IEF 7. 2D PAGE 8. Western Blot


An introduction to the use of molecular genetics in plant breeding


An introduction to the use of molecular genetics in plant breeding


An introduction to the use of molecular genetics in plant breeding


An introduction to the use of molecular genetics in plant breeding


An introduction to the use of molecular genetics in plant breeding


An introduction to the use of molecular genetics in plant breeding




An introduction to the use of molecular genetics in plant breeding


An introduction to the use of molecular genetics in plant breeding




An introduction to the use of molecular genetics in plant breeding


An introduction to the use of molecular genetics in plant breeding


An introduction to the use of molecular genetics in plant breeding






An introduction to the use of molecular genetics in plant breeding


Enzymes in DNA replication

Helicase unwinds parental double helix

Binding proteins stabilize separate strands

DNA polymerase DNA polymerase I (Exonuclease) removes binds nucleotides RNA primer and inserts to form new strands the correct bases

Primase adds short primer to template strand

Ligase joins Okazaki fragments and seals other nicks in sugar23 phosphate backbone





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