Motility Test and Maintenance Medium Use: For the detection of motility in Gram-negative nonfermentative
Motility Test HiVeg Medium (Edwards and Ewing HiVeg Medium)
bacteria.
Motility Nitrate Medium, Buffered Composition per liter: Peptone.......................................................................................... 5.0g Galactose....................................................................................... 5.0g Agar .............................................................................................. 3.0g Beef extract ................................................................................... 3.0g Na2HPO4 ....................................................................................... 2.5g KNO3 ............................................................................................ 1.0g Glycerin .....................................................................................5.0mL pH 7.3 ± 0.1 at 25°C
Preparation of Medium: Add components, except agar, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until dissolved. Add agar. Gently heat until boiling. Distribute into tubes in 11.0mL volumes. Autoclave for 15 min at 15 psi pressure– 121°C. If not used within 4 hr, heat tubes to 100°C for 10 min.
Use: For the cultivation and observation of the motility of Clostridium perfringens.
1225
Composition per liter: Plant peptone .............................................................................. 10.0g NaCl.............................................................................................. 5.0g Agar .............................................................................................. 4.0g Plant extract .................................................................................. 3.0g pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 4.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For testing motility of enteric bacteria.
Motility Test HiVeg Medium with Triphenyltetrazolium Chloride (Edwards and Ewing HiVeg Medium) Composition per liter:
Motility Sulfide Medium Composition per liter: Gelatin......................................................................................... 80.0g Proteose peptone ......................................................................... 10.0g NaCl .............................................................................................. 5.0g Agar .............................................................................................. 4.0g Beef extract ................................................................................... 3.0g Sodium citrate ............................................................................... 2.0g L-Cystine ....................................................................................... 0.2g Ferrous ammonium citrate ............................................................ 0.2g pH 7.3 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Diagnostic Systems.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes in 4–5.0mL volumes. Autoclave for 15 min at 10 psi pressure–116°C.
Plant peptone .............................................................................. 10.0g NaCl.............................................................................................. 5.0g Agar .............................................................................................. 4.0g Plant extract .................................................................................. 3.0g Triphenyltetrazolium chloride solution .....................................5.0mL pH 7.2 ± 0.2 at 25°C
Source: This medium, without triphenyltetrazolium chloride solution, is available as a premixed powder from HiMedia.
Triphenyltetrazolium Choride Solution: Composition per 5.0mL: Triphenyltetrazolium chloride ...................................................... 0.1g
Preparation of Triphenyltetrazolium Choride Solution: Add triphenyltetrazolium chloride to distilled/deionized water and bring volume to 5.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except triphenyltetra-
teria to produce H2S from L-cystine. For the differentiation of Gramnegative bacteria of the Enterobacteriaceae.
zolium chloride solution, to distilled/deionized water and bring volume to 1.0L.Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 5.0mL triphenyltetrazolium chloride solution. Mix thoroughly. Aseptically distribute into tubes.
Motility Test HiVeg Medium
Motility Test and Maintenance Medium
Use: For the determination of bacterial motility and the ability of bac-
Composition per liter: Plant hydrolysate No. 1............................................................... 10.0g Agar .............................................................................................. 5.0g NaCl .............................................................................................. 5.0g pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Composition per liter: Peptone ....................................................................................... 10.0g NaCl.............................................................................................. 5.0g Agar .............................................................................................. 4.0g Beef extract................................................................................... 3.0g 2,3,5-Triphenyltetrazolium chloride ........................................... 0.05g
Media.
Preparation of Medium: Add components to distilled/deionized
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into screwcapped tubes in 8.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 4.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the detection of bacterial motility. © 2010 by Taylor and Francis Group, LLC
Use: For the cultivation, maintenance, and observation of the motility of Listeria monocytogenes.