Starch Casein Agar
MgSO4·7H2O................................................................................ 0.3g Calcium chloride solution ........................................................10.0mL Trace elements solution ...........................................................10.0mL
Calcium Chloride Solution: Composition per 100.0mL: CaCl2·2H2O .................................................................................. 3.0g
Preparation of Calcium Chloride Solution: Add CaCl2·2H2O
to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Trace Elements Solution: Composition per liter: FeSO4·7H2O ................................................................................. 0.5g CoCl2 ............................................................................................ 0.2g MnSO4·H2O................................................................................ 0.16g ZnSO4·7H2O ............................................................................... 0.14g
Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Mineral Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Aspergillus awamori.
Starch Agar with Bromcresol Purple Composition per liter: Agar ............................................................................................ 15.0g Cornstarch ................................................................................... 10.0g Meat peptone............................................................................... 10.0g Bromcresol Purple solution .......................................................1.2mL pH 6.8 ± 0.2 at 25°C
Bromcresol Purple Solution: Composition per 10.0mL: Bromcresol Purple ...................................................................... 0.16g Ethanol (95% solution) ............................................................10.0mL
Preparation of Bromcresol Purple Solution: Add Bromcresol Purple to 10.0mL of 95% ethanol. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the differentiation of Gardnerella vaginalis (Haemophilus vaginalis, Corynebacterium vaginale) from other microorganisms found in the genitourinary tract, with the exception of some strains of Streptococcus and Lactobacillus. Differentiation is based on starch hydrolysis. Bacteria that can hydrolyze starch appear as colonies surrounded by a yellow zone.
Starch Agar with Bromcresol Purple Composition per liter: Solution 1 ...............................................................................200.0mL Solution 2.................................................................................20.0mL pH 7.8 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC
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Solution 1: Composition per 200.0mL: Heart infusion agar ....................................................................5.0mL Bromcresol Purple solution .......................................................0.2mL
Preparation of Solution 1: Add components to distilled/deionized water and bring volume to 200.0mL. Mix thoroughly. Gently heat while stirring and bring to boiling. Heart Infusion Agar: Composition per liter: Beef heart, solids from infusion................................................ 500.0g Agar ............................................................................................ 15.0g Tryptose ...................................................................................... 10.0g NaCl.............................................................................................. 5.0g
Preparation of Heart Infusion Agar: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling.
Bromcresol Purple Solution: Composition per 10.0mL: Bromcresol Purple ...................................................................... 0.16g Ethanol (95% solution) ............................................................10.0mL
Preparation of Bromcresol Purple Solution: Add Bromcresol Purple to 10.0mL of ethanol. Mix thoroughly.
Solution 2: Composition per 20.0mL: Starch ............................................................................................ 0.4g
Preparation of Solution 2: Add starch to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Gently heat while stirring and bring to boiling.
Preparation of Medium: Combine solution 1 and solution 2. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the differentiation of Gardnerella vaginalis (Haemophilus vaginalis, Corynebacterium vaginale) from other microorganisms found in the genitourinary tract, with the exception of some strains of Streptococcus and Lactobacillus. Differentiation is based on starch hydrolysis. Bacteria that can hydrolyze starch appear as colonies surrounded by a yellow zone.
Starch Agar Medium for Pseudomonas Composition per liter: Agar ............................................................................................ 15.0g Peptone ......................................................................................... 5.0g Yeast extract.................................................................................. 5.0g Soluble starch................................................................................ 3.0g pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Pseudomonas species and Erwinia herbicola.
Starch Casein Agar Composition per liter: Agar ............................................................................................ 15.0g Soluble starch.............................................................................. 10.0g K2HPO4......................................................................................... 2.0g