Handbookmicrobiologicalmedia parte5 by Marco Acuña

Starch Casein Agar

MgSO4·7H2O................................................................................ 0.3g Calcium chloride solution ........................................................10.0mL Trace elements solution ...........................................................10.0mL

Calcium Chloride Solution: Composition per 100.0mL: CaCl2·2H2O .................................................................................. 3.0g

Preparation of Calcium Chloride Solution: Add CaCl2·2H2O

to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Trace Elements Solution: Composition per liter: FeSO4·7H2O ................................................................................. 0.5g CoCl2 ............................................................................................ 0.2g MnSO4·H2O................................................................................ 0.16g ZnSO4·7H2O ............................................................................... 0.14g

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Mineral Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly.

Use: For the cultivation and maintenance of Aspergillus awamori.

Starch Agar with Bromcresol Purple Composition per liter: Agar ............................................................................................ 15.0g Cornstarch ................................................................................... 10.0g Meat peptone............................................................................... 10.0g Bromcresol Purple solution .......................................................1.2mL pH 6.8 ± 0.2 at 25°C

Bromcresol Purple Solution: Composition per 10.0mL: Bromcresol Purple ...................................................................... 0.16g Ethanol (95% solution) ............................................................10.0mL

Preparation of Bromcresol Purple Solution: Add Bromcresol Purple to 10.0mL of 95% ethanol. Mix thoroughly.

Use: For the differentiation of Gardnerella vaginalis (Haemophilus vaginalis, Corynebacterium vaginale) from other microorganisms found in the genitourinary tract, with the exception of some strains of Streptococcus and Lactobacillus. Differentiation is based on starch hydrolysis. Bacteria that can hydrolyze starch appear as colonies surrounded by a yellow zone.

Starch Agar with Bromcresol Purple Composition per liter: Solution 1 ...............................................................................200.0mL Solution 2.................................................................................20.0mL pH 7.8 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

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Solution 1: Composition per 200.0mL: Heart infusion agar ....................................................................5.0mL Bromcresol Purple solution .......................................................0.2mL

Preparation of Solution 1: Add components to distilled/deionized water and bring volume to 200.0mL. Mix thoroughly. Gently heat while stirring and bring to boiling. Heart Infusion Agar: Composition per liter: Beef heart, solids from infusion................................................ 500.0g Agar ............................................................................................ 15.0g Tryptose ...................................................................................... 10.0g NaCl.............................................................................................. 5.0g

Preparation of Heart Infusion Agar: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling.

Preparation of Bromcresol Purple Solution: Add Bromcresol Purple to 10.0mL of ethanol. Mix thoroughly.

Solution 2: Composition per 20.0mL: Starch ............................................................................................ 0.4g

Preparation of Solution 2: Add starch to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Gently heat while stirring and bring to boiling.

Preparation of Medium: Combine solution 1 and solution 2. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or distribute into sterile tubes.

Starch Agar Medium for Pseudomonas Composition per liter: Agar ............................................................................................ 15.0g Peptone ......................................................................................... 5.0g Yeast extract.................................................................................. 5.0g Soluble starch................................................................................ 3.0g pH 7.0 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Pseudomonas species and Erwinia herbicola.

Starch Casein Agar Composition per liter: Agar ............................................................................................ 15.0g Soluble starch.............................................................................. 10.0g K2HPO4......................................................................................... 2.0g

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Starch Casein Potassium Nitrate Agar

KNO3 ............................................................................................ 2.0g NaCl .............................................................................................. 2.0g Casein............................................................................................ 0.3g MgSO4·7H2O .............................................................................. 0.05g CaCO3 ......................................................................................... 0.02g FeSO4·7H2O................................................................................ 0.01g

Starch Solution: Composition per 20.0mL:

Preparation of Medium: Add components to distilled/deionized

Preparation of Medium: Combine 5.0mL of heart infusion broth,

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. For bottom layers, distribute into tubes in 15.0mL volumes. For top layers, distribute into tubes in 17.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and enumeration of Actinomycetes species

Starch ............................................................................................ 0.4g

Preparation of Starch Solution: Add starch to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Gently heat while stirring and bring to boiling. 0.2mL of Bromcresol Purple solution, 200.0mL of distilled/deionized water, and 20.0mL of starch solution. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Corynebacterium species.

from water and soil samples by the double-layer agar technique.

Starch Casein Potassium Nitrate Agar Composition per liter: Agar ............................................................................................ 18.0g Starch .......................................................................................... 10.0g KNO3 ............................................................................................ 2.0g K2HPO4 ......................................................................................... 2.0g NaCl .............................................................................................. 2.0g Casein............................................................................................ 0.3g MgSO4·7H2O .............................................................................. 0.05g CaCO3 ......................................................................................... 0.02g FeSO4·7H2O................................................................................ 0.01g

Use: For the selective isolation and cultivation of streptomycetes.

Starch Fermentation Broth Composition per 225.2mL: Starch solution .........................................................................20.0mL Heart infusion broth ...................................................................5.0mL Bromcresol Purple solution .......................................................0.2mL pH 7.8 ± 0.2 at 25°C

Heart Infusion Broth: Composition per liter: Beef heart, infusion from .......................................................... 500.0g Tryptose ...................................................................................... 10.0g NaCl .............................................................................................. 5.0g pH 7.4 ± 0.2 at 25°C

Starch HiVeg Agar Composition per liter: Agar ............................................................................................ 15.0g Plant peptone ................................................................................ 5.0g NaCl.............................................................................................. 5.0g Starch, soluble............................................................................... 2.0g Yeast extract.................................................................................. 1.5g Plant extract .................................................................................. 1.5g pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Use: For the cultivation and differentiation of a variety of microorganisms based on amylase production. After incubation, starch hydrolysis is determined by the addition of Gram’s or Lugol’s iodine solution. Organisms that produce amylase appear as colonies surrounded by a clear zone.

Starch Hydrolysis Agar Composition per liter: Beef heart, infusion from.......................................................... 500.0g Soluble starch.............................................................................. 20.0g Agar ............................................................................................ 15.0g Tryptose ...................................................................................... 10.0g NaCl.............................................................................................. 5.0g pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

Source: Heart infusion broth is available as a premixed powder from

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

BD Diagnostic Systems.

Use: For the cultivation and differentiation of a variety of microorgan-

Preparation of Heart Infusion Broth: Add components to dis-

isms based on amylase production. After incubation, starch hydrolysis is determined by the addition of Gram’s or Lugol’s iodine solution. Organisms that produce amylase appear as colonies surrounded by a clear zone.

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Bromcresol Purple Solution: Composition per 10.0mL: Bromcresol Purple ........................................................................ 0.1g Ethanol (95% solution) ............................................................10.0mL

Starch Hydrolysis Agar Composition per liter: Agar ............................................................................................ 12.0g Soluble starch.............................................................................. 10.0g Beef exract ................................................................................... 3.0g pH 7.5 ± 0.2 at 25°C

Starkey’s Medium C, Modified Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and differentiation of a variety of microorganisms based on amylase production. For the differentiation of bacteria, e.g. Neisseria sp. based upon starch hydrolysis. After incubation, starch hydrolysis is determined by the addition of Gram’s or Lugol’s iodine solution. Organisms that produce amylase appear as colonies surrounded by a clear zone.

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Preparation of Trace Salts Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Saccharomonospora halophila (Microbispora sp.).

Starch Salts Agar Composition per liter:

Starch Medium Composition per liter: Agar ............................................................................................ 15.0g Soluble starch.............................................................................. 10.0g Yeast extract.................................................................................. 3.0g MgSO4·7H2O .............................................................................. 0.25g

Use: For the cultivation and maintenance of Guignardia laricina.

Starch Mineral Salt Agar Composition per liter: Agar ............................................................................................ 12.0g Starch, soluble............................................................................. 10.0g CaCO3 ........................................................................................... 2.0g (NH4)2SO4 ..................................................................................... 2.0g K2HPO4 ......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 1.0g NaCl .............................................................................................. 1.0g pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Streptoverticillium species and Thermomonospora formosensis.

Starch Nitrate Medium (DSMZ Medium 856) Composition per liter:

Agar ............................................................................................ 20.0g Solution A..............................................................................500.0mL Solution B ..............................................................................500.0mL

Solution A: Composition per 500.0mL: CaCO3 ........................................................................................... 2.0g (NH4)2SO4 .................................................................................... 2.0g K2HPO4......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 1.0g NaCl.............................................................................................. 1.0g Trace salts ..................................................................................1.0mL

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Trace Salts: Composition per 100.0mL: FeSO4·7H2O.................................................................................. 0.1g MnCl2·4H2O ................................................................................. 0.1g ZnSO4·7H2O ................................................................................. 0.1g

Preparation of Trace Salts: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Solution B: Composition per 500.0mL: Soluble starch.............................................................................. 10.0g

Preparation of Solution B: Make a paste of the starch with a small volume of distilled/deionized water and then gradually add the starch to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Thoroughly mix 500.0mL of solution A and 500.0mL of solution B. Adjust the pH to 7.2. Add 20.0g of agar and gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Thoroughly mix to evenly distribute the precipitate. Pour into sterile Petri dishes or distribute into sterile tubes.

NaCl .......................................................................................... 100.0g Agar ............................................................................................ 20.0g Starch .......................................................................................... 20.0g CaCO3 ........................................................................................... 3.0g KNO3 ............................................................................................ 2.0g K2HPO4 ......................................................................................... 1.0g MgSO4 .......................................................................................... 0.5g Trace salts solution ....................................................................1.0mL pH 7.1 ± 0.2 at 25°C

Use: For the cultivation of Amycolatopsis rugosa, Nocardia lucida, Streptomyces canus, Streptomyces cyanogriseus, Streptomyces fradiae, Streptomyces hiroshimensis, Streptomyces kuwaitiensis, Streptomyces rubroverrucosus, Streptomyces spinoverrucosus, Streptomyces viridoverrucosus, and Thermoactinomyces dichotomicus.

Trace Salts Solution: Composition per 100.0mL:

Composition per liter:

FeSO4·7H2O.................................................................................. 0.1g MnCl2·4H2O.................................................................................. 0.1g ZnSO4·7H2O ................................................................................. 0.1g © 2010 by Taylor and Francis Group, LLC

Starkey’s Medium C, Modified Sodium lactate .............................................................................. 3.5g MgSO4·7H2O ................................................................................ 2.0g Na2SO4 .......................................................................................... 1.0g NH4Cl ........................................................................................... 1.0g

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Starkey’s Medium C, Modified with Salt

Yeast extract.................................................................................. 1.0g KH2PO4 ......................................................................................... 0.5g CaCl2·2H2O................................................................................... 0.1g Ferrous ammonium sulfate solution ........................................50.0mL L-Cysteine·HCl·H2O solution ..................................................10.0mL pH 7.5 ± 0.2 at 25°C

Ferrous Ammonium Sulfate Solution: Composition per 100.0mL: Fe(NH4)2(SO4)2·6H2O .................................................................. 1.0g

Preparation of Ferrous Ammonium Sulfate Solution: Add Fe(NH4)2(SO4)2·6H2O to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. L-Cysteine·HCl·H2O Solution: Composition per 10.0mL: L-Cysteine·HCl·H2O ................................................................... 0.75g

Preparation of L-Cysteine·HCl·H2O Solution: Add L-cyste-

ine·HCl·H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except ferrous ammonium sulfate solution and L-cysteine·HCl·H2O solution, to tap water and bring volume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile ferrous ammonium sulfate solution and 10.0mL of sterile L-cysteine·HCl·H2O solution. Mix thoroughly. Adjust pH to 7.5 with filter-sterilized 2N NaOH. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Desulfotomaculum species and Desulfovibrio species.

Starkey’s Medium C, Modified with Salt Composition per liter: NaCl ............................................................................................ 25.0g Sodium lactate............................................................................... 3.5g MgSO4·7H2O ................................................................................ 2.0g Na2SO4 .......................................................................................... 1.0g NH4Cl ........................................................................................... 1.0g Yeast extract.................................................................................. 1.0g KH2PO4 ......................................................................................... 0.5g CaCl2·2H2O................................................................................... 0.1g Ferrous ammonium sulfate solution ........................................50.0mL L-Cysteine·HCl·H2O solution ..................................................10.0mL pH 7.5 ± 0.2 at 25°C

Ferrous Ammonium Sulfate Solution: Composition per 100.0mL: Fe(NH4)2(SO4)2·6H2O .................................................................. 1.0g

Preparation of Ferrous Ammonium Sulfate Solution: Add Fe(NH4)2(SO4)2·6H2O to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. L-Cysteine·HCl·H2O

Solution: Composition per 10.0mL:

to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile ferrous ammonium sulfate solution and 10.0mL of sterile L-cysteine·HCl·H2O solution. Mix thoroughly. Adjust pH to 7.5 with filter-sterilized 2N NaOH. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of halophilic Desulfovibrio species.

Steenken and Smith Agar (Hohn’s Medium, Modified) Composition per 2065.0mL: Homogenized whole egg .....................................................1500.0mL Stock salts solution ................................................................500.0mL Lacmoid solution .....................................................................25.0mL HCl (1N solution) ....................................................................40.0mL pH 6.6 ± 0.2 at 25°C

Stock Salts Solution: Composition per 500.0mL: KH2PO4......................................................................................... 2.0g Asparagine .................................................................................... 1.5g Magnesium citrate ..................................................................... 1.25 g Na2HPO4, anhydrous ................................................................... 1.2 g MgSO4 .......................................................................................... 0.3g Glycerol ...................................................................................60.0mL

Preparation of Stock Salts Solution: Add components, except glycerol, to distilled/deionized water that has been warmed to 80°C. Bring volume to 440.0mL. Mix thoroughly. Add 60.0mL of glycerol. Mix thoroughly. Autoclave for 20 min at 10 psi pressure–115°C. Cool to 25°C. Aseptically divide the solution into two 250.0mL parts. Lacmoid Solution: Composition per 100.0mL: Lacmoid ........................................................................................ 1.0g Ethanol (50% solution) ..........................................................100.0mL

Preparation of Lacmoid Solution: Add lacmoid to 100.0mL of ethanol solution. Mix thoroughly.

Preparation of Medium: To one 250.0mL part of sterile stock salts solution, add 25.0mL of lacmoid solution. Mix thoroughly. To the other 250.0mL part of sterile stock salts solution, add 40.0mL of HCl solution. Mix thoroughly. Soak eggs in 70% ethanol for 10 min. Dry between sterile towels. Break eggs into a sterile container. Aseptically homogenize the whole eggs with a sterile glass rod. Add both stock salt solutions to the homogenized whole egg mixture. Mix thoroughly. Filter through sterile cheesecloth. Aseptically distribute into sterile tubes. Inspissate medium at 85°C (moist heat) for 90 min on 2 consecutive days.

Use: For the cultivation and maintenance of Mycobacterium microti.

Sterility Test Broth (USP Alternative Thioglycolate Medium) Composition per liter:

Preparation of Medium: Add components, except ferrous ammo-

Pancreatic digest of casein.......................................................... 15.0g Glucose ......................................................................................... 5.0g Yeast extract.................................................................................. 5.0g NaCl.............................................................................................. 2.5g L-Cystine ....................................................................................... 0.5g Sodium thioglycolate .................................................................... 0.5g pH 7.1 ± 0.2 at 25°C

nium sulfate solution and L-cysteine·HCl·H2O solution, to tap water and bring volume to 940.0mL. Mix thoroughly. Gently heat and bring

agnostic Systems.

L-Cysteine·HCl·H2O ................................................................... 0.75g

Preparation of L-Cysteine·HCl·H2O Solution: Add L-cyste-

ine·HCl·H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Source: This medium is available as a premixed powder from BD Di-

STL Broth Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. If not used immediately, prior to inoculation heat tubes in a boiling water bath for 5–10 min. Cool to 25°C.

Use: As an alternate medium, instead of fluid thioglycolate broth, for testing the sterility of a variety of specimens.

Stetteria Medium (DSMZ Medium 795) Composition per liter: Sulfur, powdered......................................................................... 10.0g Peptone.......................................................................................... 2.0g Yeast extract.................................................................................. 1.0g KH2PO4 ......................................................................................... 0.5g NaHCO3 ...................................................................................... 0.16g NiCl2·6H2O ................................................................................ 3.0mg Resazurin ................................................................................. 0.75mg Synthetic seawater, concentrated ...........................................500.0mL Trace elements solution ...........................................................15.0mL Na2S·9H2O solution .................................................................10.0mL Selenite-tungstate solution.........................................................1.5mL pH 7.2 ± 0.2 at 25°C

Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.5g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Selenite-Tungstate Solution Composition per liter: NaOH ............................................................................................ 0.5g Na2WO4·2H2O ........................................................................... 4.0mg Na2SeO3·5H2O........................................................................... 3.0mg

Preparation of Selenite-Tungstate Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Synthetic Seawater, Concentrated: Composition per liter: NaCl ............................................................................................ 55.4g MgSO4·7H2O .............................................................................. 14.0g MgCl2·6H2O................................................................................ 11.0g CaCl2·2H2O................................................................................... 1.5g KCl................................................................................................ 1.3g NaBr.............................................................................................. 0.2g H3BO3 ......................................................................................... 0.06g SrCl2·6H2O.................................................................................. 0.03g Na3-citrate ................................................................................ 20.0mg KI .............................................................................................. 0.1mg

Preparation of Synthetic Seawater, Concentrated: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

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MnSO4·2H2O ................................................................................ 0.5g CoSO4·7H2O............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Mix thoroughly. Preparation of Medium: Prepare and dispense medium under 80% N2 + 20% CO2 gas mixture. Add components, except synthetic seawater and Na2S·9H2O solution, to 490.0mL distilled/deionized water. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 500.0mL filtersterilized concentrated seawater. Flush with 80% N2 + 20% CO2 gas mixture for 20 min. Aseptically add 10.0mL Na2S·9H2O solution. Adjust pH to 6.0 with H2SO4. Mix thoroughly. Aseptically and anaerobically distribute 20mL aliquots into sterile 100mL serum bottles. Pressurize bottles to 2 bar gas overpressure with 80% N2 + 20% CO2. Heat at 100°C for 1.5 h. Before use check that the medium pH is 6.0.

Use: For the cultivation of Stetteria hydrogenophila and Staphylothermus hellenicus.

STL Broth Composition per liter: Casamino acids ............................................................................. 1.0g Glucose ........................................................................................ 1.0g Sodium glutamate ......................................................................... 1.0g CaCl2·2H2O .................................................................................. 0.1g KNO3 ............................................................................................ 0.1g MgSO4·7H2O ................................................................................ 0.1g Sodium glycerophosphate............................................................. 0.1g Thiamine .................................................................................... 1.0mg Vitamin B12 ................................................................................. 1.0μg Trace elements solution .............................................................1.0mL pH 7.5 ± 0.2 at 25°C

Trace Elements Solution: Composition per liter: Disodium EDTA ........................................................................... 8.0g MnCl2·4H2O ................................................................................. 0.1g CoCl2·6H2O ................................................................................ 0.02g KBr ............................................................................................. 0.02g KI ................................................................................................ 0.02g ZnCl2 ........................................................................................... 0.02g CuSO4 ......................................................................................... 0.01g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O .......................................................................... 0.01g LiCl ............................................................................................ 5.0mg SnCl2·2H2O................................................................................ 5.0mg

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

1640

Stock Culture Agar

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation and cultivation of Sphaerotilus natans.

Stonebrink’s Medium

Use: For the isolation and cultivation of Cytophaga species, Herpetosiphon species, Saprospira species, and Flexithrix species.

Stock Culture Agar Composition per liter: Beef heart infusion.................................................................... 500.0g Gelatin......................................................................................... 10.0g Proteose peptone ......................................................................... 10.0g Agar .............................................................................................. 7.5g Casein............................................................................................ 5.0g Na2HPO4 ....................................................................................... 4.0g Sodium citrate ............................................................................... 3.0g Glucose ......................................................................................... 0.5g pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to cold distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the maintenance of pathogenic and nonpathogenic bacteria, especially streptococci.

Stock Culture Agar with L-Asparagine Composition per liter: Beef heart infusion.................................................................... 500.0g Gelatin......................................................................................... 10.0g Proteose peptone ......................................................................... 10.0g Agar .............................................................................................. 7.5g Casein............................................................................................ 5.0g Na2HPO4 ....................................................................................... 4.0g Sodium citrate ............................................................................... 3.0g L-Asparagine ................................................................................. 1.0g Glucose ......................................................................................... 0.5g pH 7.5 ± 0.2 at 25°C

Composition per 3040.0mL: Homogenized whole egg ..............................................................2.0L Mineral salts solution....................................................................1.0L Malachite Green solution.........................................................40.0mL

Mineral Salts Solution: Composition per liter: Na-pyruvate ................................................................................ 12.5g KH2PO4......................................................................................... 7.0g Na2HPO4·7H2O............................................................................. 4.0g

Preparation of Mineral Salts Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.

Malachite Green Solution: Composition per 100.0mL: Malachite Green............................................................................ 2.0g

Preparation of Malchite Green Solution: Add Malachite Green to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.

Homogenized Whole Egg: Composition per 2.0L: Whole eggs ................................................................................ 36–48

Preparation of Homogenized Whole Egg: Use fresh eggs, less than 1 week old. Scrub the shells with soap. Let stand in a soap solution for 30 min. Rinse in running water. Soak eggs in 70% ethanol for 15 min. Break the eggs into a sterile container. Homogenize by shaking. Filter through four layers of sterile cheesecloth into a sterile graduated cylinder. Measure out 2.0L. Preparation of Medium: Aseptically add 40.0mL sterile Malachite Green solution to 1.0L of sterile mineral salts solution. Mix thoroughly. Aseptically add 2.0L of homogenized whole egg. Mix thoroughly. Distribute into sterile screw-capped tubes. Place tubes in a slanted position. Inspissate at 85°C (moist heat) for 45 min. Use: For the cultivation of Mycobacterium species. For the isolation of Mycobacterium bovis.

Use: For the maintenance of pathogenic and nonpathogenic bacteria, especially streptococci.

Stokes Agar Composition per liter: Agar ............................................................................................ 12.5g Glucose ......................................................................................... 1.0g Peptone.......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.2g CaCl2 ........................................................................................... 0.05g FeCl3·6H2O ................................................................................. 0.01g

Straw DYAA Composition per liter: Agar ............................................................................................ 20.0g Glucose ....................................................................................... 10.0g Yeast extract.................................................................................. 1.0g Asparagine .................................................................................... 0.5g K2HPO4·3H2O .............................................................................. 0.5g MgSO4·7H2O .............................................................................. 0.25g FeCl3 solution.............................................................................0.5mL Straw ....................................................................................... variable

FeCl3 Solution: Composition per 10.0mL: FeCl3 ............................................................................................. 1.0g

Preparation of FeCl3 Solution: Add FeCl3 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Preparation of Medium: Add components, except straw, to distilled/ deionized water and bring volume to 1.0L. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Autoclave

Streptococcus agalactiae Selective HiVeg Agar Base with Blood and Staphylococcus B toxin

1641

straw for 15 min at 15 psi pressure–121°C. Aseptically add straw to the solidified agar.

Quadrant II: Composition per 5.0mL:

Use: For the cultivation of Cochliobolus sativus.

TSA II agar ................................................................................5.0mL Sheep blood, defibrinated ........................................................0.25mL

Straw Malt Agar Composition per liter: Agar ............................................................................................ 15.0g Malt extract ................................................................................. 10.0g Straw ....................................................................................... variable

Preparation of Medium: Add components, except straw, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Autoclave straw for 15 min at 15 psi pressure–121°C. Aseptically add some straw to the solidified agar.

Use: For the cultivation of Cladosporium vignae, Cochliobolus sativus, and Cochliobolus victoriae.

StrepB Carrot Broth™ Composition per liter: Proteose peptone No. 3 ............................................................... 25.0g Soluble sStarch............................................................................ 20.0g Selective agents........................................................................... 12.2g Morpholinepropanesulfonic aAcid (MOPS)............................... 11.0g Na2HPO4 ....................................................................................... 8.5g Glucose ......................................................................................... 2.5g Sodium pyruvate ........................................................................... 1.0g MgSO4 ........................................................................................ 20.0g StrepB carrot broth tiles with growth promoting factors ........ variable pH 7.4 ± 0.1 at 25°C

Quadrant III: Composition per 5.0mL: Bile esculin agar ........................................................................5.0mL

Quadrant IV: Composition per 5.0mL: Blood agar base with 6.5% NaCl...............................................5.0mL

Preparation of Quadrant Media: Sterilize agars by autoclaving for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Add additional components as filter sterilized solutions. Mix and distribute as 5.0mL aliquots into quadrants. Use: For the differentiation and presumptive identification of streptococci. The Strep (Streptococcus) ID (Identification) Quad Plate is a four-sectored plate, each containing a different medium.

Streptococcal Growth Medium Composition per liter: Beef heart, solids from infusion................................................ 500.0g Tryptose ...................................................................................... 10.0g NaCl.............................................................................................. 5.0g Glucose ......................................................................................... 1.0g Bromcresol Purple solution .......................................................1.0mL pH 7.4 ± 0.2 at 25°C

Preparation of Bromcresol Purple Solution: Add Bromcresol

Source: This medium is available from Hardy Diagnostics.

Purple to 10.0mL of ethanol. Mix thoroughly.

Preparation: This medium is supplied as a prepared broth in tubes.

Preparation of Medium: Add components to distilled/deionized

The StrepB carrot broth tile is added to a tube just prior to inoculation with a vaginal swab. The tile must remain submerged in the broth.

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes in 5.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For detecting the presence of Group B Streptococcus infections

Use: For the cultivation of Streptococcus species and other Gram-pos-

in pregnant women. This new screening test is an improvement over conventional methods, by increasing sensitivity, decreasing turn around time, while lowering overall cost. Tubes show an orange to red color change, typical of group B streptococci. The production of orange, red, or brick red pigment is a unique characteristic of hemolytic Group B streptococci due to reaction with substrates such as starch, proteose peptone, serum, and folate pathway inhibitors.

Strep ID Quad Plate Composition per liter: Quadrant I ..................................................................................5.0mL Quadrant II .................................................................................5.0mL Quadrant III................................................................................5.0mL Quadrant IV ...............................................................................5.0mL

Source: Available as a prepared medium from BD Diagnostic Sys-

itive cocci. Growth in this medium turns the indicator yellow and the solution turbid.

Streptococcus agalactiae Selective HiVeg Agar Base with Blood and Staphylococcus B toxin Composition per liter: Agar ............................................................................................ 13.0g Plant peptone .............................................................................. 10.0g NaCl.............................................................................................. 5.0g Plant extract No. 1 ........................................................................ 5.0g Esculin .......................................................................................... 1.0g Thallous sulfate......................................................................... 0.333g Crystal Violet ................................................................................ 1.3g Sheep blood, defibrinated ........................................................50.0mL Staphylococcus B toxin............................................................25.0mL pH 7.0 ± 0.2 at 25°C

tems.

Source: This medium, without blood or toxin, is available as a pre-

Quadrant I: Composition per 5.0mL:

mixed powder from HiMedia.

Bacitracin ................................................................................... 0.5mg TSA II agar ................................................................................5.0mL

staphylococcal toxin, to distilled/deionized water and bring volume to 925.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave

Preparation of Medium: Add components, except sheep blood and

1642

Streptococcus Agar

for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile sheep blood and 25.0mL of staphylococcal toxin. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the selective cultivation of Streptococcus agalactiae.

Streptococcus Agar Composition per liter: Glucose ....................................................................................... 20.0g Pancreatic digest of casein .......................................................... 20.0g Agar ............................................................................................ 15.0g K2HPO4 ......................................................................................... 2.0g MgSO4·7H2O ................................................................................ 0.1g pH 6.8 ± 0.2 at 25°C

NaCl.............................................................................................. 5.0g Synthetic detergent ....................................................................... 3.0g Esculin .......................................................................................... 1.0g Sodium citrate............................................................................... 1.0g Ferric ammonium citrate............................................................... 0.5g NaN3 ........................................................................................... 0.25g pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Caution: Sodium azide is toxic. Azides also react with metals and disposal must be highly diluted.

Use: For the selective isolation, cultivation, and enumeration of streptococci from specimens containing a mixed flora.

Use: For the cultivation and maintenance of Streptococcus species.

Streptococcus faecalis Broth See: SF Broth

Streptococcus Blood Agar, Selective Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein .......................................................... 10.0g Beef extract ................................................................................... 6.7g Nucleic acid .................................................................................. 6.0g NaCl .............................................................................................. 5.0g Sheep blood, defibrinated ........................................................50.0mL Maltose solution.......................................................................10.0mL Antibiotic inhibitor ..................................................................10.0mL pH 7.3 ± 0.2 at 25°C

Maltose Solution: Composition per 10.0mL: Maltose................................................................................. 0.25–5.0g

Preparation of Maltose Solution: Add maltose to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Antibiotic Inhibitor: Composition per 10.0mL: Polymyxin B sulfate.................................................................... 0.02g Neomycin sulfate ........................................................................ 0.01g

Preparation of Antibiotic Inhibitor: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components—except sheep blood, maltose solution, and antibiotic inhibitor—to distilled/deionized water and bring volume to 930.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile sheep blood, sterile maltose solution, and sterile antibiotic inhibitor. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of group A hemolytic Streptococcus species from the human respiratory tract.

Streptococcus Enrichment HiVeg Broth (SE HiVeg Broth) Composition per liter: Plant hydrolysate......................................................................... 26.0g Yeast extract.................................................................................. 6.0g © 2010 by Taylor and Francis Group, LLC

Streptococcus lactis Differential HiVeg Agar Base with Potassium Ferricyanide and Citrate Composition per liter: Agar ............................................................................................ 15.0g Skim milk (nonfat milk) ............................................................. 10.0g Glucose ......................................................................................... 5.0g Plant hydrolysate No. 3................................................................. 2.5g Potassium ferricyanide solution...............................................10.0mL Citrate solution.........................................................................10.0mL pH 6.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Citrate Solution: Composition per 10.0mL: Ferric citrate................................................................................ 0.25g Sodium citrate............................................................................. 0.25g

Preparation of Citrate Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sterilize using flowing steam for 30 min.

Potassium Ferricyanide Solution: Composition per 10.0mL: K3[Fe(CN)6] ................................................................................. 1.0g

Preparation of Potassium Ferricyanide Solution: Add 1.0g of K3[Fe(CN)6] to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sterilize using flowing steam for 30 min.

Preparation of Medium: Add components, except potassium ferricyanide and citrate solution, to distilled/deionized water and bring volume to 980.0L. Mix thoroughly. Gently heat until boiling. Autoclave for 12 min at 10 psi pressure–115°C. Cool to 50°C. Add 10.0mL sterile potassium ferricyanide solution and 10.0mL citrate solution. Mix thoroughly. Pour into sterile Petri dishes or aseptically distribute into sterile tubes.

Use: For the differentiation of citrate-utilizing lactic streptococci— Lactobacillus lactis (Streptococcus lactis) subspecies diacetylactis— from citrate-nonutilizing Lactobacillus lactis (Streptococcus lactis) and Lactobacillus lactis (Streptococcus lactis) subspecies cremoris.

Streptococcus Selection HiVeg Brot

Streptococcus Medium Composition per liter: Agar ............................................................................................ 15.0g Glucose ......................................................................................... 4.0g K2HPO4 ......................................................................................... 3.8g Pancreatic digest of casein ............................................................ 2.5g Yeast extract.................................................................................. 2.5g pH 7.6 ± 0.2 at 25°C

1643

NaCl.............................................................................................. 4.0g Sodium citrate............................................................................... 1.0g L-Cystine....................................................................................... 0.2g NaN3 ............................................................................................. 0.2g Na2SO3 ......................................................................................... 0.2g Crystal Violet ............................................................................. 0.2mg pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-

Preparation of Medium: Add components to distilled/deionized

Media.

Preparation of Medium: Add components to distilled/deionized

Use: For the cultivation and maintenance of Enterococcus faecalis.

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the selective isolation and enumeration of all types of strep-

Streptococcus mutans Medium

tococci including group A beta hemolytic strains.

Composition per 100.0mL: Pancreatic digest of casein ............................................................ 2.0g Mannitol........................................................................................ 0.5g NaCl ............................................................................................ 0.25g Lactoalbumin .............................................................................. 0.25g Agar .......................................................................................... 0.075g L-Cystine ..................................................................................... 0.05g Sodium thioglycolate .................................................................. 0.05g Thallium acetate........................................................................ 0.025g Crystal Violet ............................................................................. 0.1mg Bromcresol Purple (0.04% solution) .......................................15.0mL pH 7.1 ± 0.2 at 25°C

Caution: Thallium salts are toxic. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 5.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.

Streptococcus Selection HiVeg Agar with Cycloheximide Composition per liter: Agar ............................................................................................ 15.0g Plant hydrolysate ........................................................................ 15.0g Glucose ......................................................................................... 5.0g Papaic digest of soybean meal...................................................... 5.0g NaCl.............................................................................................. 4.0g Sodium citrate............................................................................... 1.0g NaN3 ............................................................................................. 0.2g Na2SO3 ......................................................................................... 0.2g L-Cystine....................................................................................... 0.2g Crystal Violet ................................................................................ 0.2g Cycloheximide solution ...........................................................10.0mL pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-

Use: For the selective isolation and cultivation of Streptococcus

Media.

mutans. Bacteria that turn the medium yellow are presumptive for Streptococcus mutans.

Cycloheximide Solution: Composition per 10.0mL: Cycloheximide............................................................................ 0.01g

Streptococcus pneumoniae Medium Composition per liter:

Preparation of Cycloheximide Solution: Add cycloheximide to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Pancreatic digest of casein .......................................................... 17.0g Glucose ....................................................................................... 10.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g Yeast extract.................................................................................. 3.0g K2HPO4 ........................................................................................ 2.5g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except cycloheximide solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asptically add 10.0mL cycloheximide solution. Mix thoroughly. Pour into sterile Petri dishes or aseptically distribute into tubes.

Preparation of Medium: Add components to distilled/deionized

Use: For the selective isolation and enumeration of all types of strep-

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation of Streptococcus pneumoniae.

Streptococcus Selection HiVeg Agar Composition per liter: Agar ............................................................................................ 15.0g Plant hydrolysate......................................................................... 15.0g Glucose ......................................................................................... 5.0g Papaic digest of soybean meal ...................................................... 5.0g © 2010 by Taylor and Francis Group, LLC

tococci including group A beta hemolytic strains.

Streptococcus Selection HiVeg Broth Composition per liter: Plant hydrolysate ........................................................................ 15.0g Glucose ......................................................................................... 5.0g Papaic digest of soybean meal...................................................... 5.0g NaCl.............................................................................................. 4.0g Sodium citrate............................................................................... 1.0g L-Cystine....................................................................................... 0.2g NaN3 ............................................................................................. 0.2g

1644

Streptococcus Selective Medium

Na2SO3.......................................................................................... 0.2g Crystal Violet ............................................................................. 0.2mg pH 7.4 ± 0.2 at 25°C

add 50.0mL of sterile bovine serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Streptococcus suis.

Source: This medium is available as a premixed powder from HiMedia.

Streptococcus thermophilus Agar (ST Agar)

Use: For the selective cultivation of streptococci including group A beta hemolytic strains.

Streptococcus Selective Medium Composition per liter: Special peptone ........................................................................... 23.0g Agar ............................................................................................ 10.0g NaCl .............................................................................................. 5.0g Starch ............................................................................................ 1.0g Horse blood, defibrinated ........................................................50.0mL Antibiotic inhibitor ..................................................................10.0mL pH 7.3± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid

Composition per liter: Agar ............................................................................................ 15.0g Sucrose........................................................................................ 10.0g Pancreatic digest of casein.......................................................... 10.0g Yeast extract.................................................................................. 5.0g K2HPO4......................................................................................... 2.0g pH 6.8 ± 0.2 at 25°C

Use: For the isolation and cultivation of Streptococcus thermophilus from dairy products.

Streptococcus thermophilus Isolation HiVeg Agar Composition per liter:

Colistin sulfate ......................................................................... 10.0mg Oxolinic acid.............................................................................. 5.0mg

Agar ............................................................................................ 15.0g Plant hydrolysate ........................................................................ 10.0g Sucrose........................................................................................ 10.0g Yeast extract.................................................................................. 5.0g K2HPO4......................................................................................... 2.0g pH 6.8 ± 0.2 at 25°C

Preparation of Antibiotic Inhibitor: Add components to dis-

Source: This medium is available as a premixed powder from Hi-

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Media.

Preparation of Medium: Add components to distilled/deionized

Preparation of Medium: Add components, except horse blood and

Unipath.

Antibiotic Inhibitor: Composition per 10.0mL:

antibiotic inhibitor, to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile horse blood and sterile antibiotic inhibitor. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation of streptococci from clinical specimens or foodstuffs.

Streptococcus suis Medium Composition per liter: Peptone........................................................................................ 10.0g Meat extract .................................................................................. 8.0g Glucose ......................................................................................... 5.0g Lactose .......................................................................................... 5.0g Yeast extract.................................................................................. 3.0g K2HPO4 ......................................................................................... 2.5g KH2PO4 ......................................................................................... 2.5g L-Cysteine·HCl.............................................................................. 0.5g MgSO4·7H2O ................................................................................ 0.2g MnSO4·4H2O .............................................................................. 0.05g Bovine serum ...........................................................................50.0mL pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components, except bovine serum, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically © 2010 by Taylor and Francis Group, LLC

Use: For the selective isolation and cultivation of Streptococcus thermophilus.

Streptococcus uberis Broth Composition per liter: Peptone ......................................................................................... 0.5g Yeast extract.................................................................................. 0.5g Skimmed milk...............................................................................1.0L

Use: For the cultivation and maintenance of Streptococcus uberis.

Streptomyces Agar (LMG Medium 93) Composition per liter: Agar ............................................................................................ 20.0g L-Asparagine................................................................................. 1.0g K2HPO4......................................................................................... 1.0g Glycerol ...................................................................................10.0mL Trace salts solution ....................................................................1.0mL pH 7.0–7.4

Streptomycete Antibiotic Activity Medium

Trace Salts Solution: Composition per 100.0mL: FeSO4·7H2O.................................................................................. 0.1g MnCl2·4H2O.................................................................................. 0.1g ZnSO4·7H2O ................................................................................. 0.1g

Preparation of Trace Salts Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Preparation of Medium: Add components, except agar, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH (use pH indicator paper) to 7.2 using KOH. Add agar. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Stygiolobus azoricus.

Use: For the cultivation of Streptomyces spp.

Streptomyces Agar Composition per liter: Agar ............................................................................................ 20.0g Glucose ....................................................................................... 10.0g Beef extract ................................................................................... 4.0g Peptone.......................................................................................... 4.0g NaCl .............................................................................................. 2.5g Yeast extract.................................................................................. 1.0g pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Actinomadura ferruginea,

1645

Streptomyces Medium Composition per liter: Agar ............................................................................................ 25.0g Glucose ......................................................................................... 5.0g L-Glutamic acid............................................................................. 4.0g KH2PO4......................................................................................... 1.0g NaCl.............................................................................................. 1.0g MgSO4·7H2O ................................................................................ 0.7g FeSO4·7H2O............................................................................... 3.0mg pH 7.0 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Streptomyces kanamyceticus.

Streptomycete Antibiotic Activity Inoculum Medium Composition per liter:

Actinomadura libanotica, Actinomadura madurae, Actinomadura pelletieri, Actinomadura roseoviolacea, Actinomadura spiralis, Actinomadura verrucosospora, Micropolyspora fascifera, Nocardioides albus, Nocardiopsis albus, Nocardiopsis dassonvillei, Saccharopolyspora rectivirgula, Streptococcus pluton, and Streptomyces griseus.

Pancreatic digest of casein.......................................................... 10.0g D-Glucose ...................................................................................... 5.0g Yeast extract.................................................................................. 5.0g K2HPO4......................................................................................... 1.0g Liver extract...........................................................................100.0mL pH 6.9 ± 0.2 at 25°C

Streptomyces Agar

Preparation of Medium: Add components to distilled/deionized

Composition per liter: Agar ............................................................................................ 12.0g Malt extract ................................................................................. 10.0g Glucose ......................................................................................... 4.0g Yeast extract.................................................................................. 4.0g CaCO3 ........................................................................................... 2.0g

Use: For the cultivation and maintenance of Actinoplanes missouriensis, Saccharopolyspora rectivirgula, Streptococcus albogriseolus, Streptomyces badius, Streptomyces bikiniensis, and Thermomonospora mesophila.

Streptomyces Agar Composition per liter: Agar ............................................................................................ 12.0g Malt extract ................................................................................. 10.0g Glucose ......................................................................................... 4.0g Yeast extract.................................................................................. 4.0g CaCO3 ........................................................................................... 2.0g pH 7.2 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Streptomyces species to be used in the antibiotic activity assay.

Streptomycete Antibiotic Activity Medium Composition per liter: Agar ............................................................................................ 15.0g D-Glucose .................................................................................... 15.0g Soybean meal.............................................................................. 15.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 1.0g CaCO3 ........................................................................................... 1.0g Glycerol .....................................................................................2.5mL pH 6.8 ± 0.2 at 25°C

Use: For the the cultivation and determination of antibiotic production of Streptomyces species by the streak method. Bacillus subtilis NRRL B-765, Sarcina lutea NRRL B-1018, Escherichia coli NRRL B-766, Saccharomyces pasteurianus NRRL Y-139, Candida albicans NRRL

1646

Streptomycete Medium

Y-477, and Mucor ramannianus NRRL 1839 are used as test organisms.

Preparation of Trace Elements Solution: Add components to

Streptomycete Medium

Preparation of Medium: Add components to distilled/deionized

Composition per liter: Solution B ..............................................................................500.0mL Solution A ..............................................................................400.0mL Solution C ..............................................................................100.0mL

Solution A: Composition per 400.0mL: Glucose ....................................................................................... 20.0g Agar .............................................................................................. 4.0g Yeast extract.................................................................................. 1.2g MgSO4·7H2O .............................................................................. 0.25g Bromcresol Purple .................................................................... 0.012g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Solution B: Composition per 500.0mL: Na2HPO4·2H2O...................................................................... 534.0mg KH2PO4 .................................................................................. 272.0mg

distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 1.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and differentiation of streptomycetes based on their reduction of nitrate to nitrite. Bacteria that reduce nitrate to nitrite form a red color after the addition of Griess-Ilosvay reagent.

Streptomycete Medium Composition per liter: Agar ............................................................................................ 12.0g NaCl.............................................................................................. 5.0g Na2HPO4·2H2O........................................................................... 1.98g KH2PO4....................................................................................... 1.51g Glucose ......................................................................................... 1.0g Pancreatic digest of casein............................................................ 1.0g MgSO4·7H2O ................................................................................ 0.5g Phenol Red................................................................................ 0.012g Urea solution..........................................................................100.0mL pH 6.8 ± 0.2 at 25°C

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Urea Solution: Composition per 100.0mL:

Solution C: Composition per 100.0mL:

Preparation of Urea Solution: Add urea to distilled/deionized wa-

CaCO3 ........................................................................................... 1.0g

Preparation of Medium: Add components, except urea solution, to

Preparation of Solution C: Add CaCO3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Preparation of Medium: Distribute solution C into test tubes in

Urea............................................................................................. 20.0g ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of sterile urea solution. Mix thoroughly. Aseptically distribute into sterile tubes. Allow tubes to cool in a slanted position.

0.2mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Combine cooled, sterile solution A and cooled, sterile solution B. Mix thoroughly. Add 1.8mL of solution A-B to each test tube containing sterile solution C. Mix thoroughly to distribute the CaCO3. Cool tubes rapidly in an ice-water bath.

Use: For the cultivation and differentiation of streptomycetes based on their ability to produce urease.

Use: For the cultivation and differentiation of streptomycetes based on

Composition per liter:

their formation of organic acids. Bacteria that form organic acids turn the medium yellow and dissolve the CaCO3.

Sodium hippurate........................................................................ 10.0g Na2HPO4 ....................................................................................... 5.0g Glucose ......................................................................................... 2.0g Meat extract .................................................................................. 2.0g Peptone ......................................................................................... 2.0g Yeast extract.................................................................................. 2.0g pH 7.0 ± 0.2 at 25°C

Streptomycete Medium Composition per liter: Glycerol ........................................................................................ 5.0g Agar .............................................................................................. 4.0g NaCl .............................................................................................. 2.0g KNO3 ............................................................................................ 1.0g Na2HPO4·2H2O......................................................................... 0.534g MgSO4·7H2O ................................................................................ 0.5g KH2PO4 ..................................................................................... 0.272g Trace elements solution .............................................................1.0mL pH 6.8 ± 0.2 at 25°C

Trace Elements Solution: Composition per 100.0mL: FeSO4·7H2O.................................................................................. 0.1g MnCl2·4H2O.................................................................................. 0.1g ZnSO4·7H2O ................................................................................. 0.1g © 2010 by Taylor and Francis Group, LLC

Streptomycete Medium

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes in 3.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and differentiation of streptomycetes based on their ability to hydrolyze hippurate.

Streptomycin Assay Agar with Yeast Extract See: Antibiotic Medium 5

Streptomycin L Broth Medium Composition per liter: Pancreatic digest of casein.......................................................... 10.0g NaCl.............................................................................................. 5.0g

Streptomycin Terramycin® Malt Extract Agar

Yeast extract.................................................................................. 5.0g Glucose ......................................................................................... 1.0g Streptomycin solution ..............................................................10.0mL pH 7.0 ± 0.2 at 25°C

Streptomycin Solution: Composition per 10.0mL:

1647

Streptomycin sulfate ................................................................ 25.0mg

Streptomycin Solution: Composition per 10.0mL:

Preparation of Streptomycin Solution: Add streptomycin sul-

Streptomycin sulfate .............................................................. 500.0mg

fate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Streptomycin Solution: Add streptomycin sul-

Preparation of Medium: Add components, except streptomycin solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Bring pH to 7.0. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0mL of sterile streptomycin solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Escherichia coli.

Streptomycin Nutrient Agar Composition per liter: Agar ............................................................................................ 15.0g Peptone.......................................................................................... 5.0g Meat extract .................................................................................. 3.0g Streptomycin sulfate ................................................................ 40.0mg

Use: For the cultivation and maintenance of Escherichia coli.

Streptomycin Nutrient Agar No. 2 Composition per liter: Agar ............................................................................................ 15.0g Peptone.......................................................................................... 5.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 2.0g Beef extract ................................................................................... 1.0g Streptomycin solution ..............................................................10.0mL pH 7.0 ± 0.2 at 25°C

fate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except streptomycin solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Bring pH to 7.0. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0mL of sterile streptomycin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation of Micrococcus luteus.

Streptomycin Nutrient Agar No. 4 Composition per liter: Agar ............................................................................................ 15.0g Peptone ......................................................................................... 5.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 2.0g Beef extract................................................................................... 1.0g Streptomycin solution ..............................................................10.0mL pH 7.0 ± 0.2 at 25°C

Streptomycin Solution: Composition per 10.0mL: Streptomycin sulfate ................................................................ 80.0mg

Preparation of Streptomycin Solution: Add streptomycin sulfate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Streptomycin Solution: Composition per 10.0mL:

Preparation of Medium: Add components, except streptomycin solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Bring pH to 7.0. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0mL of sterile streptomycin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Streptomycin sulfate .............................................................. 125.0mg

Use: For the cultivation of Corynebacterium species.

Preparation of Streptomycin Solution: Add streptomycin sulfate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except streptomycin solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Bring pH to 7.0. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0mL of sterile streptomycin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Micrococcus luteus.

Streptomycin Nutrient Agar No. 3

Streptomycin Terramycin® Malt Extract Agar Composition per liter: Malt extract................................................................................. 30.0g Agar ............................................................................................ 15.0g Peptone ......................................................................................... 5.0g Streptomycin solution ............................................................100.0mL Terramycin solution ...............................................................100.0mL pH 5.4 ± 0.2 at 25°C

Streptomycin Solution: Composition per 100.0mL: Streptomycin............................................................................... 0.07g

Composition per liter:

Preparation of Streptomycin Solution: Add streptomycin to dis-

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

1648

Streptosel™ Agar

Terramycin Solution: Composition per 100.0mL: Terramycin .................................................................................. 0.07g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 13 psi pressure–118°C.

Preparation of Terramycin Solution: Add terramycin to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Use: For the selective isolation and cultivation of streptococci from

Preparation of Medium: Add components, except streptomycin solution and terramycin solution, to distilled/deionized water and bring volume to 800.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of sterile streptomycin solution and 100.0mL of sterile terramycin solution. Mix thoroughly. Pour into sterile Petri dishes in 20.0mL volumes.

Steroidobacter Medium with Testosterone (DSMZ Medium 1116)

Use: For the cultivation and enumeration of fungi isolated from sewage and polluted waters.

Streptosel™ Agar Composition per liter: Pancreatic digest of casein .......................................................... 15.0g Agar ............................................................................................ 12.0g Glucose ......................................................................................... 5.0g Papaic digest of soybean meal ...................................................... 5.0g NaCl .............................................................................................. 4.0g Sodium citrate ............................................................................... 1.0g L-Cystine ....................................................................................... 0.2g NaN3.............................................................................................. 0.2g Na2SO3 .......................................................................................... 0.2g Crystal Violet ............................................................................. 0.2mg pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Caution: Sodium azide is toxic. Azides also react with metals and disposal must be highly diluted.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. If medium is used the same day, do not autoclave. Pour into sterile Petri dishes or leave in tubes. If medium is to be stored, autoclave for 15 min at 13 psi pressure–118°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the selective isolation, cultivation, and enumeration of streptococci from specimens containing a mixed flora.

Streptosel™ Broth Composition per liter: Pancreatic digest of casein .......................................................... 15.0g Glucose ......................................................................................... 5.0g Papaic digest of soybean meal ...................................................... 5.0g NaCl .............................................................................................. 4.0g Sodium citrate ............................................................................... 1.0g L-Cystine ....................................................................................... 0.2g Na2SO3 .......................................................................................... 0.2g NaN3.............................................................................................. 0.2g Crystal Violet ............................................................................. 0.2mg pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

specimens containing a mixed flora.

Composition per 1040.3mL: NaCl ............................................................................................. 1.0g KCl ............................................................................................... 0.5g NaNO3 ........................................................................................ 0.42g MgCl2·6H2O ................................................................................. 0.4g NH4Cl ........................................................................................ 0.25g KH2PO4 ........................................................................................ 0.2g CaCl2·2H2O ................................................................................ 0.15g Na2SO4 ........................................................................................ 0.07g Bicarbonate solution ................................................................30.0mL Testosterone solution ...............................................................10.0mL Trace elements solution SL-10 ..................................................0.1mL Selenite-tungstate solution.........................................................0.1mL Vitamin solution.........................................................................0.1mL pH 7.2 ± 0.2 at 25°C

Testosterone Solution: Composition per 10.0mL: Testosterone .................................................................................. 0.2g Acetone ....................................................................................10.0mL

Preparation of Testosterone Solution: Add tesosterone to acetone adn bring volume to 10.0mL. Mix thoroughly.

Selenite-Tungstate Solution: Composition per liter: NaOH............................................................................................ 0.5g Na2WO4·2H2O ........................................................................... 4.0mg Na2SeO3·5H2O........................................................................... 3.0mg

Preparation of Selenite-Tungstate Solution: Add components to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure– 121°C. Trace Elements Solution SL-10: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl.............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoSO4·7H2O ............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O ............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O ............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Trace Elements Solution SL-10: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled/ deionized water to 1.0L. Mix thoroughly. Adjust pH to 7.0.

Steroidobacter Medium with Heptanoate

Vitamin Solution: Composition per liter: Vitamin B12 .............................................................................. 50.0mg Thiamine-HCl·2H2O ................................................................ 50.0mg Riboflavin ................................................................................ 50.0mg D-Ca-pantothenate.................................................................... 50.0mg p-Aminobenzoic acid............................................................... 50.0mg Lipoic acid ............................................................................... 50.0mg Nicotinic acid ........................................................................... 25.0mg Nicotine amide ......................................................................... 25.0mg Biotin ....................................................................................... 20.0mg Folic acid.................................................................................. 20.0mg Pyridoxine-HCl ........................................................................ 10.0mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly for several hours. Filter sterilize.

Bicarbonate Solution: Composition per 100.0mL: NaHCO3 ........................................................................................ 8.4g

Preparation of Bicarbonate Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Sparge with 20% CO2 + 80% H2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Preparation of Medium: Dispense testosterone solution into anaerobic culture vessels. Allow solvent to evaporate to dryness. Add remaining components, except bicarbonate solution, vitamin solution, trace elements solution SL-10, and selenite-tungstate solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Dispense 10.0mL portions of the medium to the culture vessels. Sparge with a gas mixture of 80% N2 + 20% CO2. Close the culture vessels. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. For every 10.0mL of medium, add 0.3mL bicarbonate solution, 0.001mL trace elements solution SL-10, 0.001mL selenite-tungstate solution, and 0.001mL vitamin solution. Adjust pH to 7.2. Treat the vessels in an ultrasonic bath to detach and suspend the testosterone.

Use: For the cultivation of Steroidobacter denitrificans.

Steroidobacter Medium with Heptanoate (DSMZ Medium 1116) Composition per 1090.3mL: NaCl ............................................................................................. 1.0g KCl ............................................................................................... 0.5g NaNO3......................................................................................... 0.42g MgCl2·6H2O.................................................................................. 0.4g NH4Cl ........................................................................................ 0.25g KH2PO4 ........................................................................................ 0.2g CaCl2·2H2O................................................................................. 0.15g Na2SO4 ........................................................................................ 0.07g Heptanoate solution .................................................................50.0mL Bicarbonate solution ................................................................30.0mL Trace elements soltuion SL-10 ..................................................0.1mL Selenite-tungstate solution.........................................................0.1mL Vitamin solution.........................................................................0.1mL pH 7.2 ± 0.2 at 25°C

1649

Preparation of Heptanoate Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly for several hours. Filter sterilize.

Preparation of Selenite-Tungstate Solution: Add components to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure– 121°C. Trace Elements Solution SL-10: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl.............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoSO4·7H2O............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Vitamin Solution: Composition per liter: Vitamin B12 .............................................................................. 50.0mg Thiamine-HCl·2H2O................................................................ 50.0mg Riboflavin ................................................................................ 50.0mg D-Ca-pantothenate ................................................................... 50.0mg p-Aminobenzoic acid............................................................... 50.0mg Lipoic acid ............................................................................... 50.0mg Nicotinic acid........................................................................... 25.0mg Nicotine amide......................................................................... 25.0mg Biotin ....................................................................................... 20.0mg Folic acid ................................................................................. 20.0mg Pyridoxine-HCl........................................................................ 10.0mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly for several hours. Filter sterilize. Bicarbonate Solution: Composition per 100.0mL: NaHCO3 ........................................................................................ 8.4g

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/

deionized water and bring volume to 100.0mL. Mix thoroughly. Sparge with 20% CO2 + 80% N2. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to room temperature.

Preparation of Medium: Add components, except heptanoate solution, bicarbonate solution, vitamin solution, trace elements solution, and selenite-tungstate solution, to distilled/deionized water and bring

1650

STTA Medium

volume to 1.0L. Mix thoroughly. Dispense 10.0mL portions of the medium to the culture vessels. Sparge with a gas mixture of 80% N2 + 20% CO2. Close the culture vessels. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. For every 10.0mL of medium, add 0.5mL heptanoate solution, 0.3mL bicarbonate solution, 0.001mL trace elements solution SL-10, 0.001mL selenite-tungstate solution, and 0.001mL vitamin solution. Adjust pH to 7.2. Treat the vessels in an ultrasonic bath to detach and suspend the heptanoate.

Use: For the cultivation of Steroidobacter denitrificans. STT Agar See: Sucrose Teepol Tellurite Agar

STTA Medium Composition per liter: Peptone........................................................................................ 20.0g Glycerol ...................................................................................... 15.0g Agar ............................................................................................ 13.0g Yeast extract.................................................................................. 2.0g K2HPO4 ......................................................................................... 1.0g MgSO4·4H2O ................................................................................ 1.0g Antibiotic solution ...................................................................10.0mL Thallous acetate solution .........................................................10.0mL pH 7.0 ± 0.2 at 25°C

Antibiotic Solution: Composition per 10.0mL: Streptomycin sulfate ..................................................................... 0.5g Cycloheximide ............................................................................ 0.05g

Preparation of Antibiotic Solution: Add cycloheximide and streptomycin sulfate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Thallous Acetate Solution: Composition per 10.0mL: Thallous acetate .......................................................................... 0.05g

Preparation of Thallous Acetate Solution: Add thallous acetate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except antibiotic solution and thallous acetate solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°– 50°C. Aseptically add sterile antibiotic solution and thallous acetate solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation and cultivation of Brochothrix thermosphacta.

Stuart Leptospira Broth, Modified Composition per liter: NaCl ............................................................................................ 1.93g Na2HPO4 ..................................................................................... 0.66g NH4Cl ......................................................................................... 0.34g MgCl2·6H2O................................................................................ 0.19g L-Asparagine ............................................................................... 0.13g KH2PO4 ....................................................................................... 0.08g Glycerol .....................................................................................5.0mL Rabbit serum, inactivated at 56°C, 30 min ............................100.0mL pH 7.4 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

Preparation of Medium: Add each component, except rabbit serum, to distilled/deionized water in separate flasks and bring each volume to 100.0mL. Mix thoroughly. Combine the seven solutions, except the rabbit serum. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile rabbit serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Leptospira species.

Stuart Medium Base Composition per 1100.0mL: NaCl.............................................................................................. 1.8g Na2HPO4 ..................................................................................... 0.67g MgCl2·6H2O ............................................................................... 0.41g NH4Cl ......................................................................................... 0.27g Asparagine .................................................................................. 0.13g KH2PO4....................................................................................... 0.09g Phenol Red.................................................................................. 0.01g Glycerol .....................................................................................5.0mL Leptospira enrichment ...........................................................100.0mL pH 7.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems. Leptospira enrichment contains rabbit serum and hemoglobin and is available from BD Diagnostic Systems.

Preparation of Medium: Add components, except glycerol and Leptospira enrichment, to distilled/deionized water and bring volume to 995.0mL. Mix thoroughly. Add glycerol. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add Leptospira enrichment. Mix thoroughly. Aseptically distribute into sterile screw-capped tubes in 10.0mL volumes. Use: For the cultivation of Leptospira species.

Stuart Transport Medium Composition per liter: Sodium glycerophosphate........................................................... 10.0g Sodium thioglycolate .................................................................... 1.0g CaCl2·2H2O .................................................................................. 0.1g Methylene Blue.......................................................................... 2.0mg pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into 7.0mL screw-capped tubes. Fill tubes to capacity. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the preservation of Neisseria species and other fastidious organisms during their transport from clinic to laboratory.

Stuart Transport Medium, Modified Composition per liter: Sodium glycerophosphate........................................................... 10.0g Agar .............................................................................................. 5.0g L-Cysteine·HCl·H2O ..................................................................... 0.5g Sodium thioglycolate .................................................................... 0.5g CaCl2·2H2O .................................................................................. 0.1g Methylene Blue.......................................................................... 1.0mg pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath.

Succinate Mineral Medium Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into 7.0mL screw-capped tubes. Fill tubes to capacity. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the preservation of Neisseria species and other fastidious organisms during their transport from clinic to laboratory.

Stygiolobus Medium Composition per liter: Sulfur flowers ............................................................................... 5.0g (NH4)2SO4 ..................................................................................... 1.3g KH2PO4 ....................................................................................... 0.28g MgSO4·7H2O .............................................................................. 0.25g Yeast extract.................................................................................. 0.2g CaCl2·2H2O................................................................................. 0.07g FeCl3·6H2O ................................................................................. 0.02g Na2B4O7·10H2O......................................................................... 4.5mg MnCl2·4H2O............................................................................... 1.8mg Resazurin ................................................................................... 0.5mg ZnSO4·7H2O ............................................................................ 0.22mg CuCl2·2H2O ............................................................................. 0.05mg Na2MoO4·2H2O ....................................................................... 0.03mg VOSO4·2H2O ........................................................................... 0.03mg CoSO4 ...................................................................................... 0.01mg pH 2.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 2.5 with 10N H2SO4. Distribute 20.0mL of medium into 100.0mL flasks or serum bottles. Autoclave for 15 min at 15 psi pressure–121°C. After inoculation, pressurize bottles to 200KPa with 80%H2 + 20% CO2 .

Use: For the cultivation and maintenance of Stygiolobus azoricus.

Styrene Mineral Salts Agar Composition per liter: Agar ............................................................................................ 20.0g (NH4)2SO4 .................................................................................... 2.0g K2HPO4 ...................................................................................... 1.55g NaH2PO·2H2O............................................................................ 0.85g MgCl2·6H2O ................................................................................. 0.1g EDTA ....................................................................................... 10.0mg FeSO4·7H2O .............................................................................. 5.0mg ZnSO4 ........................................................................................ 2.0mg CaCl2·2H2O ............................................................................... 1.0mg MnCl2·2H2O .............................................................................. 1.0mg CoCl2·6H2O ............................................................................... 0.4mg CuSO4·5H2O.............................................................................. 0.2mg Na2MoO4·2H2O......................................................................... 0.2mg

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Place plates in a desiccator. Add to the desiccator an open bottle containing 10.0mL of dibutyl phthalate and 200.0μl of styrene.

Use: For the cultivation of styrene-utilizing microorganisms.

1651

NaH2PO·2H2O............................................................................ 0.85g MgCl2·6H2O ................................................................................. 0.1g EDTA ....................................................................................... 10.0mg FeSO4·7H2O .............................................................................. 5.0mg ZnSO4 ........................................................................................ 2.0mg CaCl2·2H2O ............................................................................... 1.0mg MnCl2·2H2O .............................................................................. 1.0mg CoCl2·6H2O............................................................................... 0.4mg CuSO4·5H2O.............................................................................. 0.2mg Na2MoO4·2H2O......................................................................... 0.2mg Styrene .......................................................................................0.1mL

Preparation of Medium: Add components, except styrene, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Under a fume hood, aseptically add styrene to screw-capped tubes or flasks (5.0μl of styrene into 50.0mL of sterile mineral salts solution). Seal caps tightly. Use: For the cultivation and maintenance of styrene-utilizing microorganisms.

Succinate Mineral Medium Composition per 1001.0mL: MnSO4·7H2O .............................................................................. 62.0g Succinate....................................................................................... 2.7g K2HPO4·3H2O ............................................................................ 1.63g NH4Cl ......................................................................................... 1.02g KH2PO4....................................................................................... 0.39g Trace elements solution .............................................................1.0mL pH 6.8 ± 0.2 at 25°C

Trace Elements Solution: Composition per 100.0mL: Solution A................................................................................50.0mL Solution B ................................................................................50.0mL

Preparation of Trace Elements Solution: Aseptically combine 50.0mL of sterile solution A with 50.0mL of sterile solution B.

Solution A: Composition per liter: Disodium EDTA ......................................................................... 0.25g CaCl2·2H2O ................................................................................ 0.14g FeCl2·4H2O................................................................................. 0.14g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Solution B: Composition per liter: Disodium EDTA ......................................................................... 0.25g H3BO3 ...................................................................................... 30.0mg CoCl2·2H2O ............................................................................. 28.0mg ZnCl2 .......................................................................................... 5.0mg MnCl2·4H2O .............................................................................. 4.0mg NaMoO4·2H2O........................................................................... 3.0mg NiCl2·6H2O.............................................................................. 1.65mg CuCl2·2H2O ............................................................................... 0.7mg

Preparation of Solution B: Add components to distilled/deionized Composition per liter:

water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

(NH4)2SO4 .................................................................................... 2.0g K2HPO4 ...................................................................................... 1.55g

Preparation of Medium: Add components, except trace elements solution, to distilled/deionized water and bring volume to 1.0L. Mix

Styrene Mineral Salts Broth

1652

Succiniclasticum Medium

thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 1.0mL of sterile trace elements solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of bacteria that can utilize succinate as a carbon source.

Succiniclasticum Medium Composition per liter: Disodium succinate....................................................................... 5.0g Yeast extract.................................................................................. 5.0g NaCl ............................................................................................ 0.45g (NH4)2SO4 ................................................................................... 0.45g K2HPO4 ..................................................................................... 0.225g KH2PO4 ..................................................................................... 0.225g MgSO4·7H2O .............................................................................. 0.09g CaCl2·2H2O................................................................................. 0.06g Indigocarmine ............................................................................ 5.0mg Rumen fluid, clarified ............................................................400.0mL NaHCO3 solution .....................................................................10.0mL L-Cysteine·HCl·H2O solution ..................................................10.0mL Na2S·9H2O solution .................................................................10.0mL pH: 6.6–6.8

NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g CaCO3 ........................................................................................... 3.0g Phenylethyl alcohol ...................................................................... 3.0g MgSO4·7H2O ................................................................................ 0.5g MnSO4·4H2O ................................................................................ 0.5g Tween™ 80................................................................................... 0.1g Bromcresol Green.................................................................... 20.0mg Cycloheximide solution ...........................................................10.0mL pH 6.2 ± 0.2 at 25°C

Cycloheximide Solution: Composition per 10.0mL: Cycloheximide............................................................................ 0.01g

Preparation of Cycloheximide Solution: Add cycloheximide to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

NaHCO3 Solution: Composition per 10.0mL:

Preparation of Medium: Add agar to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring to boiling. Add remaining components, except cycloheximide solution and phenylethyl alcohol, and bring volume to 990.0mL with distilled/ deionized water. Adjust pH to 6.2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile cycloheximide solution and 3.0g of phenylethyl alcohol. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

NaHCO3 ........................................................................................ 6.4g

Use: For the isolation and cultivation of Lactobacillus species from

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

brewery isolates.

L-Cysteine·HCl·H2O

Composition per liter:

Solution: Composition per 10.0mL:

L-Cysteine·HCl·H2O ..................................................................... 0.3g

Preparation of L-Cysteine·HCl·H2O Solution: Add L-cyste-

ine·HCl·H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.3g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Before use, neutralize to pH 7.0 with sterile HCl.

Preparation of Medium: Add components, except Na2S·9H2O so-

lution, L-cysteine·HCl·H2O solution, and NaHCO3 solution, to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Gently heat and bring to boiling. Cool to room temperature while sparging with 100% CO2. Autoclave for 15 min at 15 psi pressure– 121°C. Aseptically and anaerobically add 10.0mL of sterile Lcysteine·HCl·H2O solution, 10.0mL of sterile Na2S·9H2O solution, and 10.0mL of sterile NaHCO3 solution to each tube. Mix thoroughly.

Use: For the cultivation of Succiniclasticum ruminis.

Sucrose Agar Composition per liter: Sucrose........................................................................................ 50.0g Agar ............................................................................................ 20.0g Peptone........................................................................................ 10.0g © 2010 by Taylor and Francis Group, LLC

Sucrose Agar Beef heart, solids from infusion................................................ 500.0g Sucrose........................................................................................ 50.0g Agar ............................................................................................ 15.0g Tryptose ...................................................................................... 10.0g NaCl.............................................................................................. 5.0g pH 7.4 ± 0.2 at 25°C

Use: For the differentiation of bacteria based on their ability to produce glucan. Dextran production, typical of Streptococcus sanguis and Streptococcus mutans, results in highly refractile-adherent or dry-adherent colonies. Levan production, typical of Streptococcus salivarius, results in opaque, gummy, nonadherent colonies. Colonies of Streptococcus bovis and Leuconostoc mesenteroides are similar to those of Streptococcus salivarius but are somewhat less gummy and rarely adhere to the medium. Large or small colonies that are mucoidal and nonadherent are considered negative or have no extracellular polysaccharide production.

Sucrose Broth Composition per liter: Solution A..............................................................................500.0mL Solution B ..............................................................................500.0mL pH 7.1 ± 0.2 at 25°C

Solution A: Composition per 500.0mL: Pancreatic digest of casein.......................................................... 15.0g Sodium acetate............................................................................ 12.0g

Sucrose Phosphate Transport Medium

K2HPO4 ....................................................................................... 10.0g Glucose ......................................................................................... 5.5g Yeast extract.................................................................................. 5.0g NaCl .............................................................................................. 2.5g L-Cystine ....................................................................................... 0.5g Sodium thioglycolate .................................................................... 0.5g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Solution B: Composition per 500.0mL: Sucrose........................................................................................ 50.0g

Preparation of Solution B: Add sucrose to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Preparation of Medium: Combine sterile solution A with sterile solution B. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the differentiation of bacteria based on their ability to produce glucan. Production of glucan is indicated when the broth is partially or completely gelled—typical of Streptococcus sanguis—or when gelatinous, adherent deposits form on the bottom and walls of the tube—typical of Streptococcus mutans. An increase in the viscosity indicates the production of slime (unknown polysaccharide)—typical of Streptococcus bovis.

Sucrose HiVeg Agar for Brewery Isolates Composition per liter: Sucrose........................................................................................ 50.0g Agar ............................................................................................ 15.0g Plant hydrolysate......................................................................... 10.0g (NH4)3PO4 ..................................................................................... 5.0g Yeast extract.................................................................................. 5.0g K2HPO4 ......................................................................................... 5.0g Cycloheximide solution ...........................................................10.0mL pH 6.2 ± 0.2 at 25°C

Source: This medium, without cycloheximide, is available as a premixed powder from HiMedia.

Cycloheximide Solution: Composition per 10.0mL: Cycloheximide ............................................................................ 0.01g

Preparation of Cycloheximide Solution: Add cycloheximide to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

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Sucrose Peptone Agar Composition per liter: Sucrose........................................................................................ 20.0g Agar ............................................................................................ 12.0g Peptone ......................................................................................... 5.0g K2HPO4......................................................................................... 0.5g MgSO4·7H2O .............................................................................. 0.25g pH 7.2–7.4 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.2–7.4. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Pseudomonas solanacearum and Xanthomonas albilineans.

Sucrose Peptone Medium Composition per liter: Peptone ....................................................................................... 20.0g Sucrose........................................................................................ 20.0g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Pseudomonas species.

Sucrose Phosphate Glutamate Transport Medium Composition per liter: Sucrose........................................................................................ 75.0g Na2HPO4 ..................................................................................... 1.22g Glutamic acid.............................................................................. 0.72g K2HPO4....................................................................................... 0.52g Bovine serum ...........................................................................50.0mL Antibiotic inhibitor ..................................................................10.0mL pH 7.4–7.6 at 25°C

Antibiotic Inhibitor: Composition per 10.0mL: Vancomycin .................................................................................. 0.1g Streptomycin............................................................................... 0.05g Nystatin................................................................................... 25000U

Preparation of Antibiotic Inhibitor: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except bovine serum and antibiotic inhibitor, to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.4–7.6. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile bovine serum and sterile antibiotic inhibitor. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the maintenance of Chlamydia species during transport.

Sucrose Phosphate Transport Medium Composition per liter: Sucrose........................................................................................ 68.5g K2HPO4......................................................................................... 2.1g KH2PO4......................................................................................... 1.1g

1654

Sucrose Teepol Tellurite Agar

Bovine serum ...........................................................................50.0mL Antibiotic inhibitor ..................................................................10.0mL pH 7.0 ± 0.2 at 25°C

Antibiotic Inhibitor: Composition per 10.0mL: Vancomycin .................................................................................. 0.1g Streptomycin ............................................................................... 0.05g Nystatin.................................................................................. 25,000U

Preparation of Antibiotic Inhibitor: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except bovine serum

Trace Elements Solution: Composition per liter: ZnSO4·7H2O ............................................................................... 18.0g FeSO4·7H2O.................................................................................. 9.0g MnSO4·4H2O ................................................................................ 3.0g CoCl2·6H2O .................................................................................. 0.9g CuSO4·5H2O ................................................................................. 0.8g Conc. H2SO4 ..............................................................................5.0mL

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

and antibiotic inhibitor, to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°– 50°C. Aseptically add sterile bovine serum and sterile antibiotic inhibitor. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of a variety of bacteria that can utilize sucrose as a carbon source.

Use: For the maintenance of Chlamydia species during transport.

NaCl............................................................................................ 10.0g Sodium lactate .............................................................................. 5.2g Yeast extract.................................................................................. 1.0g MgSO4·7H2O ................................................................................ 0.2g Ascorbic acid ................................................................................ 0.1g Fe(NH4)2(SO4)2·6H2O .................................................................. 0.1g K2HPO4....................................................................................... 0.01g pH 7.5 ± 0.2 at 25°C

Sucrose Teepol Tellurite Agar (STT Agar) Composition per liter: Agar ............................................................................................ 20.0g Beef extract ................................................................................... 1.0g Peptone.......................................................................................... 1.0g Sucrose.......................................................................................... 1.0g NaCl .............................................................................................. 0.5g Bromthymol Blue (0.2% solution).............................................2.5mL Tellurite solution ........................................................................2.5mL Sodium lauryl sulfate (Teepol, 0.1% solution) .......................................................0.2mL pH 8.0 ± 0.2 at 25°C

Tellurite Solution: Composition per 100.0mL: K2TeO3 ........................................................................................ 0.05g

Preparation of Tellurite Solution: Add the K2TeO3 to distilled/

deionized water and bring the volume to 100.0mL. Mix thoroughly. Filter sterilize. Use freshly prepared solution.

Caution: Potassium tellurite is toxic. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Do not autoclave. Pour into sterile Petri dishes.

Use: For the selective isolation, cultivation, and differentiation of Vibrio species based on their ability to ferment sucrose. Vibrio cholerae appears as flat yellow colonies. Vibrio parahaemolyticus appears as elevated green-yellow mucoid colonies.

Sucrose Yeast Extract Medium Composition per liter: Sucrose........................................................................................ 20.0g Yeast extract.................................................................................. 4.0g K2HPO4 ......................................................................................... 2.5g MgSO4·7H2O ................................................................................ 1.0g Trace elements solution .............................................................4.0mL pH 7.0 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

Sulfate API Broth Composition per liter:

Source: This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium: Add the components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly until dissolved. Distribute into tubes in 9.0mL volumes. Autoclave for 10 min at 15 psi pressure–121°C.

Use: For the detection, differentiation, and estimation of sulfatereducing bacteria.

Sulfate-Reducing Bacteria Enrichment Medium Composition per 1018.0mL: Solution 1...............................................................................970.0mL Solution 4.................................................................................30.0mL Solution 6A, 6B, 6C, 6D, or 6E...............................................10.0mL Solution 5...................................................................................3.0mL Solution 2...................................................................................1.0mL Solution 3...................................................................................1.0mL Solution 7...................................................................................1.0mL Solution 8...................................................................................1.0mL Solution 9...................................................................................1.0mL pH 7.2 ± 0.2 at 25°C

Solution 1: Composition per 970.0mL: Na2SO4 .......................................................................................... 3.0g NaCl.............................................................................................. 1.2g MgCl2·6H2O ................................................................................. 0.4g KCl................................................................................................ 0.3g NH4Cl ........................................................................................... 0.3g KH2PO4......................................................................................... 0.2g CaCl2·2H2O ................................................................................ 0.15g

Preparation of Solution 1: Add components to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Autoclave for 30 min at 15 psi pressure–121°C. Cool to 25°C under 90% N2 + 10% CO2.

Sulfate-Reducing Bacteria Medium

1655

Solution 2: Composition per liter:

Solution 6D: Composition per 100.0mL:

FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ................................................................................ 0.12g MnCl2·4H2O.................................................................................. 0.1g ZnCl2 ........................................................................................... 0.07g H3BO3 ......................................................................................... 0.06g Na2MoO4·2H2O ........................................................................ 0.025g NiCl2·6H2O ............................................................................... 0.025g CuCl2·2H2O .............................................................................. 0.015g HCl (25% solution) ....................................................................6.5mL

Benzoic acid.................................................................................. 5.0g

Preparation of Solution 6D: Add benzoic acid to distilled/deionized water and bring volume to 100.0mL. Adjust pH to 9.0 with NaOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Solution 6E: Composition per 100.0mL: n-Palmitic acid .............................................................................. 5.0g NaOH.......................................................................................... 0.78g

water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Preparation of Solution 6E: Add n-palmitic acid and NaOH to distilled/deionized water and bring volume to 100.0mL. Heat in a water bath until clear. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Solution 3: Composition per liter:

Solution 7: Composition per 100.0mL:

Preparation of Solution 2: Add components to distilled/deionized

NaOH ............................................................................................ 0.5g Na2SeO3 ..................................................................................... 3.0mg

Preparation of Solution 3: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Solution 4: Composition per 100.0mL: NaHCO3 ........................................................................................ 8.5g

Preparation of Solution 4: Add NaHCO3 to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Saturate with 100% CO2. Filter sterilize. Aseptically add solution to sterile, gastight, screw-capped bottles.

Solution 5: Composition per 100.0mL: Na2S·9H2O .................................................................................. 12.0g

Preparation of Solution 5: Add Na2S·9H2O to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Add solution to gas-tight, screw-capped bottles. Gas under 100% N2 for 20 min. Close caps tightly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Solution 6A: Composition per 100.0mL: Sodium acetate·3H2O.................................................................. 20.0g

Preparation of Solution 6A: Add sodium acetate·3H2O to dis-

tilled/deionized water and bring volume to 100.0mL. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Solution 6B: Composition per 100.0mL: n-Butyric acid ............................................................................... 8.0g

Preparation of Solution 6B: Add n-butyric acid to distilled/deionized water and bring volume to 100.0mL. Adjust pH to 9.0 with NaOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Thiamine ..................................................................................... 0.01g p-Aminobenzoic acid................................................................. 5.0mg Vitamin B12 ................................................................................ 5.0mg Biotin ......................................................................................... 1.0mg

Preparation of Solution 7: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Solution 8: Composition per liter: Succinic acid................................................................................. 0.6g Isobutyric acid .............................................................................. 0.5g 2-Methylbutyric acid .................................................................... 0.5g 3-Methylbutyric acid .................................................................... 0.5g Valeric acid ................................................................................... 0.5g Caproic acid .................................................................................. 0.2g

Preparation of Solution 8: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 9.0 with NaOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Solution 9: Composition per 100.0mL: Na2S2O4 ........................................................................................ 3.0g

Preparation of Solution 9: Add Na2S2O4 to 100.0mL of O2-free

distilled/deionized water. Mix thoroughly. Anaerobically filter sterilize.

Preparation Medium: To 970.0mL of cooled, sterile solution 1, aseptically and anaerobically add 1.0mL of sterile solution 2, 1.0mL of sterile solution 3, 30.0mL of sterile solution 4, and 3.0mL of sterile solution 5. Mix thoroughly. Adjust pH to 7.2 with sterile HCl solution or sterile Na2CO3 solution. Aseptically and anaerobically distribute into sterile screw-capped bottles in 100.0mL volumes. Add 1.0mL of solution 6A, 6B, 6C, 6D, or 6E to each bottle containing 100.0mL of basal medium. Add 0.1mL of solution 7, 0.1mL of solution 8, and 0.1mL of solution 9 to each bottle containing 100.0mL of basal medium. Mix thoroughly.

Use: For the isolation, cultivation, and enrichment of sulfate-reducing

Solution 6C: Composition per 100.0mL:

bacteria.

Propionic acid ............................................................................... 7.0g

Composition per 1008.0mL:

Preparation of Solution 6C: Add propionic acid to 100.0mL of

Solution A..............................................................................850.0mL Solution C ..............................................................................100.0mL Solution G................................................................................20.0mL

Sulfate-Reducing Bacteria Medium

1656

Sulfate-Reducing Bacteria Medium with Lactate

Solution D ................................................................................10.0mL Solution E (Wolfe’s vitamin solution) .....................................10.0mL Solution H ................................................................................10.0mL Solution F...................................................................................6.6mL Solution B (Trace elements solution SL-10)..............................1.0mL Solution I....................................................................................0.4mL pH 7.6 ± 0.2 at 25°C

Solution A: Composition per 920.0mL:

Thiamine·HCl ............................................................................ 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg Calcium pantothenate ................................................................ 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Thioctic acid .............................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Cyanocobalamin ........................................................................ 0.1mg

Na2SO4 .......................................................................................... 3.0g NaCl .............................................................................................. 1.0g KCl................................................................................................ 0.5g MgCl2·6H2O.................................................................................. 0.4g NH4Cl ........................................................................................... 0.3g KH2PO4 ......................................................................................... 0.2g CaCl2·2H2O................................................................................. 0.15g Resazurin ................................................................................... 0.5mg

Preparation of Solution E (Wolfe’s Vitamin Solution): Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Aseptically gas under 100% N2 for 20 min.

Preparation of Solution A: Prepare and dispense solution anaero-

Preparation of Solution F: Combine both solutions. Mix thor-

bically under 80% N2 + 20% CO2. Add components to distilled/deionized water and bring volume to 920.0mL. Mix thoroughly. Gently heat and bring to boiling. Continue boiling until resazurin turns colorless, indicating reduction, and a pH of 6.0 is reached. Cap with rubber stoppers. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Solution B (Trace Elements Solution SL-10): Composition per liter: FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ................................................................................ 0.19g MnCl2·4H2O................................................................................ 0.10g ZnCl2 ......................................................................................... 0.070g Na2MoO4·2H2O ........................................................................ 0.036g NiCl2·6H2O ............................................................................... 0.024g H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution) ..................................................................10.0mL

Preparation of Solution B (Trace Elements Solution SL-10): Add the FeCl2·4H2O to 10.0mL of HCl solution. Mix thoroughly. Bring volume to approximately 900.0mL with distilled/deionized water. Mix thoroughly. Adjust pH to 6.0 with NaOH. Bring volume to 1.0L with distilled/deionized water. Filter sterilize. Aseptically gas under 100% N2 for 20 min.

Solution C: Composition per 100.0mL: NaHCO3 ........................................................................................ 5.0g

Preparation of Solution C: Add NaHCO3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Aseptically gas under 80% N2 + 20% CO2 for 20 min.

Solution D: Composition per 10.0mL: Sodium propionate ........................................................................ 1.5g

Preparation of Solution D: Prepare and dispense solution anaerobically under 80% N2 + 20% CO2. Add sodium propionate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Cap with a rubber stopper. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Solution F: Composition per 6.6mL: AlCl3·6H2O (4.9% solution) ......................................................5.0mL Na2CO3 (10.6% solution)..........................................................1.6mL oughly. Gas with 100% N2. Cap with a rubber stopper. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Solution G: Composition per 10.0mL: Rumen fluid, clarified..............................................................20.0mL

Preparation of Solution G: Gas rumen fluid under 100% N2 for 20 min. Cap with a rubber stopper. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Solution H: Composition per 10.0mL: Na2S·9H2O.................................................................................... 0.4g

Preparation of Solution H: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Gas under 100% N2 for 20 min. Cap with a rubber stopper. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 25°C. Solution I: Composition per 10.0mL: Na2S2O4 ........................................................................................ 0.5g

Preparation of Solution I: Add Na2S2O4 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Aseptically gas under 100% N2 for 20 min. Prepare solution freshly. Preparation of Medium: To 850.0mL of cooled, sterile solution A, aseptically and anaerobically add in the following order: 1.0mL of sterile solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, 6.6mL of sterile solution F, 20.0mL of sterile solution G, and 10.0mL of sterile solution H. Mix thoroughly. Immediately prior to inoculation, aseptically and anaerobically add 0.4mL of sterile solution I. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or flasks. Use: For the cultivation and maintenance of a variety of sulfate-reducing bacteria.

Sulfate-Reducing Bacteria Medium with Lactate Composition per liter: Solution 1...............................................................................980.0mL Solution 2.................................................................................10.0mL Solution 3.................................................................................10.0mL pH 7.4 ± 0.2 at 25°C

Sulfite Agar

Solution 1: Composition per 980.0mL: Sodium lactate (70% solution)...................................................... 3.5g MgSO4·7H2O ................................................................................ 2.0g NH4Cl ........................................................................................... 1.0g Na2SO4 .......................................................................................... 1.0g Yeast extract.................................................................................. 1.0g K2HPO4 ......................................................................................... 0.5g CaCl2·2H2O................................................................................... 0.1g

Preparation of Solution 1: Add components to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.

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Part C: Composition per 100.0mL: Sodium lactate .............................................................................. 3.5g

Preparation of Part C: Add sodium lactate to distilled/deionized water and bring volume to 100.0mL. Gently heat and bring to boiling. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Aseptically combine 890.0mL part A, 100.0mL part B, and 10.0mL part C. Mix thoroughly. Distribute into screw-cap tubes. Completely fill the tubes.

Use: For the enumeration of sulfate reducing bacteria in water samples.

Solution 2: Composition per 10.0mL:

Sulfate-Reducing Medium

FeSO4·7H2O.................................................................................. 0.5g

Composition per liter:

Preparation of Solution 2: Add FeSO4·7H2O to distilled/deionized

Sodium lactate .............................................................................. 3.5g MgSO4·7H2O ................................................................................ 2.0g Peptone ......................................................................................... 2.0g Na2SO4 .......................................................................................... 1.5g Beef extract................................................................................... 1.0g K2HPO4......................................................................................... 0.5g CaCl2 ............................................................................................. 0.1g Fe(NH4)2(SO4)2·6H2O solution ...............................................10.0mL Sodium ascorbate solution.......................................................10.0mL pH 7.5 ± 0.3 at 25°C

water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.

Solution 3: Composition per 10.0mL: Ascorbic acid ................................................................................ 0.1g Sodium thioglycolate .................................................................... 0.1g

Preparation of Solution 3: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Preparation of Medium: Aseptically combine 980.0mL of cooled, sterile solution 1, 10.0mL of cooled, sterile solution 2, and 10.0mL of cooled, sterile solution 3. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the enrichment and isolation of sulfate-reducing bacteria.

Sulfate-Reducing HiVeg Medium Composition per liter: Part A .....................................................................................890.0mL Part B .....................................................................................100.0mL Part C .......................................................................................10.0mL pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Part A: Composition per 890.0mL: Plant peptone................................................................................. 2.0g MgSO4·7H2O ................................................................................ 2.0g Na2SO4 .......................................................................................... 1.5g Plant extract .................................................................................. 1.0g K2HPO4 ........................................................................................... 0.5 CaCl2 ............................................................................................. 0.1g

Preparation of Part A: Add components to distilled/deionized water and bring volume to 890.0mL. Gently heat and bring to boiling. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Part B: Composition per 100.0mL:

Fe(NH4)2(SO4)2·6H2O Solution: Composition per 100.0mL: Fe(NH4)2(SO4)2·6H2O ................................................................ 3.92g

Preparation of Fe(NH4)2(SO4)2·6H2O Solution: Add 3.92g of

Fe(NH4)2(SO4)2·6H2O to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Use freshly prepared solution.

Sodium Ascorbate Solution: Composition per 100.0mL: Sodium ascorbate........................................................................ 0.05g

Preparation of Sodium Ascorbate Solution: Add sodium ascorbate to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Use freshly prepared solution. Preparation of Medium: Add components, except sodium ascorbate solution and Fe(NH4)2(SO4)2·6H2O solution , to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Distribute into screw-capped tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Tubes must be filled to capacity after inoculation, so prepare extra medium and sterilize in a screw-capped flask or bottle. Prior to inoculation, aseptically add 0.1mL of freshly prepared sterile Fe(NH4)2(SO4)2·6H2O solution for each 10.0mL of medium in the tubes. Also aseptically add 0.1mL of freshly prepared sterile sodium ascorbate solution for each 10.0mL of medium in the tubes. Inoculate tubes. Fill tubes to capacity with extra sterile medium. Screw caps tight.

Use: For the isolation, cultivation, and enumeration of iron and sulfur bacteria.

Ferrous (NH4)2SO4 ................................................................... 0.392g Sodium ascorbate .......................................................................... 0.1g

Preparation of Part B: Add components to distilled/deionized water and bring volume to 100.0mL. Gently heat and bring to boiling. Mix thoroughly. Filter sterilize. Prepare on day of use. © 2010 by Taylor and Francis Group, LLC

Sulfite Agar Composition per liter: Agar ............................................................................................ 20.0g Pancreatic digest of casein.......................................................... 10.0g

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Sulfite HiVeg Agar with Iron

Na2SO3 .......................................................................................... 1.0g Iron nails .......................................................................................... 66 pH 7.6 ± 0.2 at 25°C

Source: This medium, without iron nails, is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into screw-capped tubes in 15.0mL volumes. Add a clean iron nail to each tube. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 45°–50°C until ready to inoculate.

Use: For the detection and cultivation of thermophilic anaerobes that can produce H2S from sulfite. Sulfite reduction appears as a blackening of the medium.

Sulfite HiVeg Agar with Iron Composition per liter: Agar ............................................................................................ 20.0g Plant hydrolysate......................................................................... 10.0g Na2SO3.......................................................................................... 1.0g Ferric citrate solution ...............................................................10.0mL pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-

FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O ............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O ............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Basal Salts Solution: Add components to 1.0L of distilled/deionized water. Adjust pH to 7.5–7.8 with NaOH. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Biotin Solution: Composition per 10.0mL:

Media.

Biotin ............................................................................................ 0.1g

Ferric Citrate Solution: Composition per 10.0mL:

Preparation of Biotin Solution: Add biotin to 10.0mL of distilled/

Ferric citrate .................................................................................. 0.5g

Preparation of Ferric Citrate Solution: Add ferric citrate to dis-

deionized water. Mix thoroughly. Filter sterilize.

Magnesium Calcium Solution: Composition per 10.0mL:

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

MgSO4·7H2O ................................................................................ 1.0g CaCl2·2H2O ................................................................................ 0.05g

Preparation of Medium: Add components to distilled/deionized

Preparation of Magnesium Calcium Solution: Add components to 10.0mL of distilled/deionized water. Mix thoroughly. Filter sterilize.

Use: For the detection of thermophilic sulfide-producing anaerobes. Sulfite Polymyxin Sulfadiazine Agar See: SPS Agar

Sulfitobacter pontiacus Medium (DSMZ Medium 733) Composition per liter: Basal salts solution.................................................................959.0mL Biotin solution..........................................................................10.0mL Na-acetate solution ..................................................................10.0mL Yeast extract peptone solution .................................................10.0mL Magnesium calcium solution ...................................................10.0mL Trace elements solution .............................................................1.0mL pH 7.6 ± 0.2 at 25°C

Na-Acetate Solution: Composition per 10.0mL: Na-acetate ..................................................................................... 1.6g

Preparation of Na-Acetate Solution: Add Na-acetate to 10.0mL of distilled/deionized water. Mix thoroughly. Filter sterilize.

Yeast Extract Peptone Solution: Composition per 10.0mL: Yeast extract.................................................................................. 1.0g Peptone ......................................................................................... 0.5g

Preparation of Yeast Extract Peptone Solution: Add components to 10.0mL of distilled/deionized water. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Preparation of Medium: Aseptically combine 959.0mL basal salts solution, 1.0mL trace elements solution, 10.0mL Na-acetate solution, 10.0mL magnesium calcium solution, 10.0mL yeast extract peptone solution, and 10.0mL biotin solution. Mix thoroughly. Aseptically distribute to sterile tubes or flasks.

Use: For the cultivation of Sulfitobacter pontiacus.

Sulfobacillus disulfidooxidans Medium (DSMZ Medium 812) Composition per liter: (NH4)2SO4 .................................................................................... 3.0g KH2PO4......................................................................................... 0.5g

Sulfolobus Broth

MgSO4·7H2O ................................................................................ 0.5g KCl................................................................................................ 0.1g Ca(NO3)2·4H2O............................................................................. 0.1g Yeast extract.................................................................................. 0.1g Glutathione solution.................................................................10.0mL pH 2.25 ± 0.1 at 25°C

Glutathione Solution: Composition per 10.0mL: Glutathione.................................................................................... 1.0g

Preparation of Glutathione Solution: Add glutathione to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except glutathione solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Adjust pH to 2.25. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to room temperature. Aseptically add 10.0mL sterile glutathione solution. Mix thoroughly. Aseptically distribute into sterile tubes or bottles.

Use: For the cultivation of Sulfobacillus disulfidooxidans.

Sulfobacillus Medium Composition per 1020.0mL: Solution A ..............................................................................700.0mL Solution B ..............................................................................300.0mL Solution C ................................................................................20.0mL pH 1.9–2.4 at 25°C

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Sulfolobus acidocaldarius Simplified Basal Medium Composition per liter: K2SO4 ........................................................................................... 6.0g NaH2PO4 ....................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.6g CaCl2·7H2O .................................................................................. 0.2g Trace minerals solution............................................................0.04mL pH 3.5 ± 0.2 at 25°C

Trace Minerals Solution: Composition per 100.0mL: FeCl3·6H2O................................................................................... 5.0g CuCl2·2H2O .................................................................................. 0.5g CoCl2·6H2O .................................................................................. 0.5g MnCl2·2H2O ................................................................................. 0.5g ZnCl2 ............................................................................................. 0.5g HCl (1N solution) ................................................................... 100.0ml

Preparation of Trace Minerals Solution: Combine components. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3.5 with H2SO4. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Sulfolobus acidocaldarius.

Sulfolobus brierleyi Medium Composition per liter:

(NH4)2SO4 ..................................................................................... 3.0g KCl................................................................................................ 0.1g K2HPO4 ......................................................................................... 0.5g MgSO4·7H2O ................................................................................ 0.5g Ca(NO3)2 ..................................................................................... 0.01g

Sulfur flowers ............................................................................. 10.0g (NH4)2SO4 .................................................................................... 3.0g K2HPO4·3H2O.............................................................................. 0.5g MgSO4·7H2O................................................................................ 0.5g KCl................................................................................................ 0.1g Ca(NO3)...................................................................................... 0.01g Yeast extract solution...............................................................20.0mL pH 1.5–2.5 at 25°C

Preparation of Solution A: Add components to distilled/deionized

Preparation of Sulfur: Autoclave sulfur at 8 psi pressure–112°C

Solution A: Composition per 700.0mL:

water and bring volume to 700.0mL. Mix thoroughly. Adjust pH to 2.0–2.2 with sulfuric acid. Autoclave for 15 min at 15 psi pressure– 121°C.

Solution B: Composition per 300.0mL: FeSO4·7H2O................................................................................ 44.2g H2SO4, 10N solution ..................................................................1.0mL

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 300.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

for 15 min.

Yeast Extract Solution: Composition per 20.0mL: Yeast extract.................................................................................. 0.2g

Preparation of Yeast Extract Solution: Add yeast extract to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Preparation of Medium: Add components, except yeast extract so-

Yeast extract.................................................................................. 0.2g

lution and sulfur, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Adjust pH with 6N H2SO4 to 1.5–2.5. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 20.0mL of sterile yeast extract solution and 10.0g of sterile sulfur. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Preparation of Solution C: Add yeast extract to distilled/deionized

Use: For the cultivation of Acidianus brierleyi.

Solution C: Composition per 20.0mL:

water and bring volume to 20.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Aseptically combine 700.0mL of solution A, 300.0mL of solution B, and 20.0mL of solution C. Aseptically adjust pH to 1.9–2.4 Use: For the cultivation of Sulfobacillus thermosulfidooxidans. © 2010 by Taylor and Francis Group, LLC

Sulfolobus Broth Composition per liter: Sucrose yeast solution............................................................500.0mL CaCl2·2H2O solution..............................................................250.0mL Trace elements solution .........................................................250.0mL pH 3.0–3.5 at 25°C

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Sulfolobus Medium

Sucrose Yeast Solution: Composition per 500.0mL: Sucrose.......................................................................................... 2.0g Yeast extract.................................................................................. 1.0g

Preparation of Sucrose Yeast Solution: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

CaCl2·2H2O Solution: Composition per 250.0mL: CaCl2·2H2O................................................................................... 2.0g

Preparation of CaCl2·2H2O Solution: Add CaCl2·2H2O to dis-

Sulfolobus Medium Composition per liter: Gellan sucrose yeast solution.................................................500.0mL CaCl2·2H2O/MgCl2·6H2O solution........................................250.0mL Trace elements solution .........................................................250.0mL pH 3.0–3.5 at 25°C

Gellan Sucrose Yeast Solution: Composition per 500.0mL: Gellan gum.................................................................................... 6.5g Sucrose.......................................................................................... 2.0g Yeast extract.................................................................................. 1.0g

tilled/deionized water and bring volume to 250.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Source: Gellan gum is available from Kelco.

Trace Elements Solution: Composition per 250.0mL:

nents to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 60°C.

(NH4)2SO4 ..................................................................................... 1.3g KH2PO4 ....................................................................................... 0.28g MgSO4·7H2O .............................................................................. 0.25g FeSO4·7H2O............................................................................. 28.0mg Na2B4O7·10H2O......................................................................... 4.5mg MnCl2·7H2O............................................................................... 1.8mg ZnSO4·7H2O ............................................................................ 0.22mg CuCl2·2H2O ............................................................................. 0.05mg NaMoO4·2H2O......................................................................... 0.03mg VOSO4·2H2O ........................................................................... 0.03mg CoSO4·2H2O ............................................................................ 0.01mg

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 250.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Aseptically combine 500.0mL of sterile sucrose yeast solution with 250.0mL of sterile CaCl2·2H2O solution and 250.0mL of sterile trace elements solution. Mix thoroughly. Adjust pH to 3.0–3.5 with sterile H2SO4. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Sulfolobus species.

Sulfolobus Medium Composition per liter: (NH4)2SO4 ..................................................................................... 1.3g Yeast extract.................................................................................. 1.0g KH2PO4 ....................................................................................... 0.28g MgSO4·7H2O .............................................................................. 0.25g CaCl2·2H2O................................................................................. 0.07g FeCl3·6H2O ................................................................................. 0.02g Na2B4O7·10H2O......................................................................... 4.5mg MnCl2·4H2O............................................................................... 1.8mg ZnSO4·7H2O ............................................................................ 0.22mg CuCl2·2H2O ............................................................................. 0.05mg Na2MoO4·2H2O ....................................................................... 0.03mg VOSO4·2H2O ........................................................................... 0.03mg CoSO4 ...................................................................................... 0.01mg pH 2.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH at 25°C to 2.0 with 10N H2SO4. Filter sterilize. Aseptically distribute into tubes or flasks.

Preparation of Gellan Sucrose Yeast Solution: Add compo-

CaCl2·2H2O/MgCl2·6H2O Solution: Composition per 250.0mL: CaCl2·2H2O ................................................................................ 2.44g MgCl2·6H2O ................................................................................. 2.0g

Preparation of CaCl2·2H2O/MgCl2·6H2O Solution: Add components to distilled/deionized water and bring volume to 250.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 60°C. Trace Elements Solution: Composition per 250.0mL: (NH4)2SO4 .................................................................................... 1.3g KH2PO4....................................................................................... 0.28g MgSO4·7H2O .............................................................................. 0.25g FeSO4·7H2O............................................................................. 28.0mg Na2B4O7·10H2O......................................................................... 4.5mg ZnSO4·7H2O ............................................................................ 0.22mg CuCl2·2H2O ............................................................................. 0.05mg NaMoO4·2H2O......................................................................... 0.03mg VOSO4·2H2O........................................................................... 0.03mg CoSO4·2H2O ............................................................................ 0.01mg

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 250.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 60°C.

Preparation of Medium: Aseptically combine 500.0mL of sterile gellan sucrose yeast solution with 250.0mL of sterile CaCl2·2H2O/ MgCl2·6H2O solution and 250.0mL of sterile trace elements solution. Mix thoroughly. Adjust pH to 3.0–3.5 with sterile H2SO4. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation of Sulfolobus species.

Sulfolobus Medium, Revised Composition per liter: (NH4)2SO4 .................................................................................... 1.3g Tryptone........................................................................................ 1.0g KH2PO4....................................................................................... 0.28g MgSO4·7H2O .............................................................................. 0.25g CaCl2·2H2O ................................................................................ 0.07g Yeast extract................................................................................ 0.05g FeCl3·6H2O ................................................................................. 0.02g Na2B4O7 ..................................................................................... 4.5mg MnCl2·4H2O .............................................................................. 1.8mg ZnSO4·7H2O ............................................................................ 0.22mg

Sulforhabdus Medium

CuCl2·H2O ............................................................................... 0.05mg Na2MoO4·H2O ......................................................................... 0.03mg VOSO4·2H2O ........................................................................... 0.03mg CoSO4 ...................................................................................... 0.01mg pH 3.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH at 25°C to 3.0 with 10N H2SO4. Filter sterilize. Aseptically distribute into tubes or flasks.

Use: For the cultivation and maintenance of Sulfolobus species.

Sulfolobus shibatae Medium Composition per liter: (NH4)2SO4 ..................................................................................... 1.3g Yeast extract.................................................................................. 1.0g KH2PO4 ....................................................................................... 0.28g MgSO4·7H2O .............................................................................. 0.25g CaCl2·2H2O................................................................................. 0.07g FeCl3·6H2O ................................................................................. 0.02g Na2B4O7·10H2O......................................................................... 4.5mg MnCl2·4H2O............................................................................... 1.8mg ZnSO4·7H2O ............................................................................ 0.22mg CuCl2·2H2O ............................................................................. 0.05mg Na2MoO4·2H2O ....................................................................... 0.03mg VOSO4·2H2O ........................................................................... 0.03mg CoSO4 ...................................................................................... 0.01mg pH 3.5 ± 0.2 at 25°C

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CaCl2·2H2O ............................................................................. 66.0mg Na2-EDTA................................................................................ 32.0mg MgSO4·7H2O ........................................................................... 31.0mg KCl........................................................................................... 31.0mg MnSO4·2H2O ............................................................................. 2.3mg ZnCl2 .......................................................................................... 2.1mg Na2B4O7·10H2O......................................................................... 1.8mg Resazurin ................................................................................... 0.5mg Serum albumin solution...........................................................10.0mL Dithiothreitol solution..............................................................10.0mL Yeast extract solution...............................................................10.0mL Iron sulfate solution ...................................................................5.0mL pH 7.6 ± 0.2 at 25°C

Dithiothreitol Solution: Composition per 10.0mL: Dithiothreitol........................................................................... 1.54mg

Preparation of Dithiothreitol Solution: Add dithiothreitol to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Iron Sulfate Solution: Composition per 10.0mL: FeSO4·7H2O................................................................................. 0.1g

Preparation of Iron Sulfate Solution: Add FeSO4·7H2O to dis-

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components to distilled/deionized

Serum Albumin Solution: Composition per 10.0mL:

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3.5 with 10N H2SO4. Filter sterilize. Aseptically distribute into tubes or flasks.

Bovine serum albumin, fraction V............................................... 1.0g

Preparation of Serum Albumin Solution: Add bovine serum al-

Use: For the cultivation of Sulfolobus shibatae.

bumin, fraction V to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Sulfolobus solfataricus Medium Composition per liter: KH2PO4 ......................................................................................... 3.1g (NH4)2SO4 ..................................................................................... 2.5g Casamino acids ............................................................................. 1.0g Yeast extract.................................................................................. 1.0g CaCl2·2H2O................................................................................. 0.25g MgSO4·7H2O ................................................................................ 0.2g Na2B4O7·10H2O......................................................................... 4.5mg MnCl2·4H2O............................................................................... 1.8mg ZnSO4·7H2O ............................................................................ 0.22mg CuCl2·2H2O ............................................................................. 0.05mg Na2MoO4·2H2O ....................................................................... 0.03mg VOSO4·2H2O ........................................................................... 0.03mg CoSO4·7H2O ............................................................................ 0.01mg pH 4.0–4.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH at 25°C to 4.0–4.2 with 10N H2SO4. Filter sterilize. Aseptically distribute into tubes or flasks.

Yeast Extract Solution: Composition per 10.0mL: Yeast extract.................................................................................. 1.0g

Preparation of Yeast Extract Solution: Add yeast extract to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature. Preparation of Medium: Prepare medium anaerobically under 100% N2 gas. Add components, except iron sulfate solution, serum albumin solution, dithiothreitol solution, and yeast extract solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Cool to 80–90°C. Adjust pH to 7.6. Cool to room temperature. Dispense 30.0mL aliquots into serum bottles. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically inject per each 30.0mL the following solutions: 0.3mL sterile yeast extract solution, 0.3mL sterile dithiothreitol solution, 0.15mL sterile iron sulfate solution, and 10.0mL sterile serum albumin solution. Final pH should be 7.6. Use: For the cultivation of Sulfophobococcus zilligii.

Use: For the cultivation and maintenance of Sulfolobus solfataricus.

Sulfophobococcus zilligii Medium (DSMZ Medium 770) Composition per 1035.0mL: Glycine.......................................................................................... 1.5g Na2CO3 .................................................................................. 230.0mg © 2010 by Taylor and Francis Group, LLC

Sulforhabdus Medium (DSMZ Medium 386a) Composition per 1002.0mL: Solution A..............................................................................920.0mL Solution C ................................................................................50.0mL Solution D................................................................................10.0mL

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Sulfur Medium

Solution E ................................................................................10.0mL Solution F.................................................................................10.0mL Solution B (Trace elements solution SL-10B).......................................1.0mL pH 7.2–7.5 at 25°C

Solution A: Composition per 920.0mL: NaCl .............................................................................................. 1.0g KCl................................................................................................ 0.5g MgCl2·6H2O.................................................................................. 0.4g KH2PO4 ......................................................................................... 0.2g NH4Cl ........................................................................................... 0.3g CaCl2·2H2O................................................................................. 0.15g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 920.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2 gas until saturated. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Solution B (Trace Elements Solution SL-10B): Composition per liter: FeCl2·4H2O ................................................................................... 1.5g H3BO3 .................................................................................... 300.0mg CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O........................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution) ....................................................................7.7mL

Preparation of Solution B (Trace Elements Solution SL-10B): Add FeCl2·4H2O to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Solution C: Composition per 100.0mL: NaHCO3 ........................................................................................ 5.0g

Preparation of Solution C: Add NaHCO3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2 gas until saturated, approximately 20 min. Filter sterilize under 100% CO2 into a sterile, gas-tight 100.0mL screw-capped bottle. Solution D: Composition per 10.0mL: Na2-acetate·3H2O.......................................................................... 0.3g

Preparation of Solution D: Add Na2-acetate to distilled/deionized

water and bring volume to 10.0mL. Sparge with N2. Filter sterilize. Store anaerobically.

Solution E: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.4g

Preparation of Solution E: Add Na2S·9H2O to distilled/deionized

water and bring volume to 10.0mL. Sparge with N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Store anaerobically.

Preparation of Solution F: Add Na-thiosulfate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Flush with 80% N2 + 20% CO2 to remove dissolved oxygen.

Preparation of Medium: Add solution B, solution C, solution D, solution E, and solution F to solution A in that order under 80% N2 + 20% CO2 gas. Adjust the pH to 7.2–7.5.

Use: For the cultivation of Desulfocapsa thiozymogenes.

Sulfur Medium Composition per liter: Sulfur, elemental ......................................................................... 10.0g KH2PO4......................................................................................... 3.0g MgSO4·7H2O ................................................................................ 0.5g (NH4)2SO4 .................................................................................... 0.3g CaCl2·2H2O ................................................................................ 0.25g FeCl3·6H2O ................................................................................. 0.02g pH 4.8 ± 0.2 at 25°C

Preparation of Medium: Add components, except sulfur, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Add 1.0g of sulfur to each of ten 250.0mL flasks. Add 100.0mL of medium to each flask. Autoclave for 30 min at 0 psi pressure–100°C on 3 consecutive days. Use: For the isolation, cultivation, and enumeration of iron and sulfur bacteria.

Sulfurimonas paralvinella Medium (DSMZ Medium 1053) Composition per liter: NaCl ........................................................................................... 20.0g Sulfur, elemental ......................................................................... 10.0g MgSO4·7H2O ................................................................................ 4.0g MgCl2·6H2O ................................................................................. 3.0g Na2S2O3·5H2O ............................................................................. 1.0g NaNO3 .......................................................................................... 1.0g CaCl2·2H2O .................................................................................. 0.8g KCl ............................................................................................. 0.33g NH4Cl ........................................................................................ 0.25g K2HPO4....................................................................................... 0.09g KH2PO4....................................................................................... 0.07g Fe2(SO4)3·H2O ............................................................................ 0.01g Resazurin .................................................................................. 0.5mg Trace elements solution ...........................................................10.0mL Bicarbonate solution ................................................................10.0mL Vitamin solution.......................................................................10.0mL Selenite-tungstate solution.........................................................1.0mL pH 6.8 ± 0.2 at 25°C

Trace Elements Solution: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl.............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoSO4·7H2O ............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O ............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g

Sulfurospirillum Medium

Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O ............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Vitamin Solution: Composition per liter: Pyridoxine-HCl ........................................................................ 10.0mg Thiamine-HCl·2H2O .................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg D-Ca-pantothenate...................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize. Bicarbonate Solution: Composition per 10.0mL: NaHCO3 ....................................................................................... 1.0g

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 20% CO2 + 80% N2. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to room temperature.

Selenite-Tungstate Solution: Composition per liter: NaOH ............................................................................................ 0.5g Na2WO4·2H2O ........................................................................... 4.0mg Na2SeO3·5H2O........................................................................... 3.0mg

Preparation of Selenite-Tungstate Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Steam sulfur for 3 hr on each of 3 successive days. Add the sulfur to the culture vessels.Add components, except vitamin solution, sulfur, and bicarbonate solution, to distilled/deionized water and bring volume to 975.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for 3 min. Cool to room temperature under 80% H2 + 20% CO2. Adjust pH to 6.8 with NaOH. Dispense under same gas atmosphere into the culture vessels containing the sulfur (up to volume of 20%). Autoclave for 20 min at 6 psi pressure–110°C. Aseptically add vitamin and bicarbonate solutions. Adjust the pH to 6.5. After inoculation pressurize vessels to 2 bar overpressure with 80% H2 + 20% CO2 gas mixture.

Use: For the cultivation of Sulfurimonas paralvinella.

Sulfurospirillum Medium Composition per 1004.0mL: Solution A ..............................................................................900.0mL Solution C ................................................................................80.0mL Solution D ................................................................................20.0mL Solution B (Trace elements solution SL-10) .............................2.0mL Solution E ..................................................................................2.0mL pH 7.2 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

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Solution A: Composition per 900.0mL: KH2PO4....................................................................................... 1.36g MgSO4·7H2O .............................................................................. 0.37g NH4Cl ......................................................................................... 0.27g CaCl2·2H2O .................................................................................. 0.1g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Anaerobically distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Solution B (Trace Elements Solution SL-10): Composition per liter: FeCl2·4H2O................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O.............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Preparation of Solution B (Trace Elements Solution SL-10): Add FeCl2·4H2O to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Solution C: Composition per 80.0mL: NaHCO3 ........................................................................................ 4.0g

Preparation of Solution C: Add NaHCO3 to distilled/deionized water and bring volume to 80.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Solution D: Composition per 20.0mL: Sodium fumarate........................................................................... 4.0g

Preparation of Solution D: Add sodium fumarate to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Solution E: Composition per 2.0mL: L-Cysteine·HCl ......................................................................... 0.063g

Preparation of Solution E: Add L-cysteine·HCl to distilled/deionized water and bring volume to 2.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: To sterile solution A in tubes or flasks, add, using a syringe, appropriate volumes of sterile solution B, solution C, solution D, and solution E. Mix thoroughly.

Use: For the cultivation of Sulfurospirillum deleyianum.

Sulfurospirillum Medium Composition per liter: Solution A..............................................................................953.0mL Solution B ................................................................................42.0mL Solution C ..................................................................................5.0mL pH 7.2 ± 0.2 at 25°C

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Sulfurospirillum II Medium

Solution A: Composition per 953.0mL: KH2PO4 ....................................................................................... 1.36g MgSO4·7H2O .............................................................................. 0.37g NH4Cl ......................................................................................... 0.27g CaCl2·2H2O................................................................................. 0.10g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 953.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature while sparging with 90% N2 + 10% CO2.

Solution B: Composition per 42.0mL: Sodium fumarate........................................................................... 4.0g NaHCO3 ........................................................................................ 2.0g Trace elements solution SL-10 ..................................................2.0mL

Trace Elements Solution SL-10: Composition per liter: FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O........................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution) ..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

Vitamin solution.......................................................................10.0mL Cysteine solution .....................................................................10.0mL Trace elements solution SL-10 ..................................................1.0mL Selenite-tungstate solution.........................................................1.0mL pH 7.3 ± 0.2 at 25°C

Cysteine Solution: Composition per 10.0mL: L-Cysteine-HCl·H2O................................................................... 0.15g

Preparation of Cysteine Solution: Add L-cysteine·HCl·H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure– 121°C. Selenite-Tungstate Solution Composition per liter: NaOH............................................................................................ 0.5g Na2WO4·2H2O ........................................................................... 4.0mg Na2SeO3·5H2O........................................................................... 3.0mg

Preparation of Selenite-Tungstate Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Na-lactate Solution: Composition per 10.0mL: Na-lactate.................................................................................... 2.25g

Preparation of Na-lactate Solution: Add Na-lactate to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly.

NaNO3 Solution: Composition per 10.0mL:

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 42.0mL. Mix thoroughly. Filter sterilize.

Preparation of NaNO3 Solution: Add NaNO3 to distilled/deion-

Solution C: Composition per 5.0mL:

NaNO3 .......................................................................................... 1.7g ized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Filter sterilize.

L-Cysteine·HCl.......................................................................... 0.063g

Na2S·9H2O Solution: Composition per 10.0mL:

Preparation of Solution C: Add L-cysteine·HCl to distilled/deion-

Na2S·9H2O.................................................................................... 0.1g

ized water and bring volume to 5.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Aseptically combine 953.0mL of sterile solution A with 42.0mL of sterile solution B and 5.0mL of sterile solution C. Prepare freshly. Adjust pH to 7.2 with sterile 2M Na2CO3 solution or sterile 2N HCl solution.

Use: For the cultivation of Sulfurospirillum deleyianum.

Sulfurospirillum II Medium (DSMZ Medium 771) Composition per 1080.0mL: Yeast extract.................................................................................. 1.0g NaCl ....................................................................................... 460.0mg K2HPO4 .................................................................................. 225.0mg KH2PO4 .................................................................................. 225.0mg (NH4)2SO4 .............................................................................. 225.0mg MgSO4·7H2O ......................................................................... 117.0mg Resazurin ................................................................................... 0.5mg NaHCO3 solution .....................................................................30.0mL NaNO3 solution ........................................................................10.0mL Na2S·9H2O solution .................................................................10.0mL Na-lactate solution ...................................................................10.0mL © 2010 by Taylor and Francis Group, LLC

NaHCO3 Solution: Composition per 30.0mL: NaHCO3 ........................................................................................ 4.2g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 30.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Filter sterilize. Trace Elements Solution SL-10: Composition per liter: FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Sulfurospirillum MV Medium Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Sparge with 80% N2 + 20% CO2.

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize. Preparation of Medium: Prepare and dispense medium under 80% N2 + 20% CO2. Add components, except NaHCO3 solution, Na2S·9H2O solution, cysteine solution, NaNO3 solution, Na-lactate solution, and vitamin solution, to distilled/deionized water and bring volume to 1.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Adjust pH to 7.3. Dispense either 10.0mL aliquots into 15mL Hungate tubes or 50.0mL aliquots into 100mL Hungate bottles. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically inject from sterile stock solutions NaHCO3 solution, Na2S·9H2O solution, cysteine solution, vitamin solution, sodium nitrate solution, and sodium lactate solution. Final pH should be 7.3.

Use: For the cultivation of Sulfurospirillum arsenophilum (Geospirillum sp.) and Sulfurospirillum barnesii.

Sulfurospirillum MV Medium (DSMZ Medium 1097) Composition per liter: NaCl ........................................................................................... 20.0g MgCl2·6H2O.................................................................................. 3.0g CaCl2·2H2O................................................................................... 0.8g KCl ............................................................................................... 0.7g NH4Cl .......................................................................................... 0.2g KH2PO4 ......................................................................................... 0.2g Resazurin .................................................................................. 0.5mg Vitamin solution.......................................................................10.0mL Sulfide solution ........................................................................10.0mL Fumarate solution ....................................................................10.0mL Bicarbonate solution ................................................................10.0mL Trace elements solution SL-10 ..................................................2.0mL Selenite-tungstate solution.........................................................1.0mL pH 7.2 ± 0.2 at 25°C

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Vitamin Solution: Composition per liter: Pyridoxine-HCl........................................................................ 10.0mg Thiamine-HCl·2H2O.................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg D-Ca-pantothenate ..................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize. Fumarate Solution: Composition per 10.0mL: Na2-fumarate ................................................................................ 1.6g

Preparation of Fumarate Solution: Add Na2-fumarate to dis-

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Trace Elements Solution SL-10: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl.............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoSO4·7H2O............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Bicarbonate Solution: Composition per 10.0mL:

Sulfide Solution: Composition per 10.0mL:

NaHCO3 ....................................................................................... 2.5g

Na2S·9H2O.................................................................................... 0.3g

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/

Preparation of Sulfide Solution: Add Na2S·9H2O to distilled/de-

deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 20% CO2 + 80% N2. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to room temperature. © 2010 by Taylor and Francis Group, LLC

ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature.

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Super HiVeg Broth

Preparation of Medium: Add components, except vitamin solution, bicarbonate solution, fumarate solution, and sulfide solution, to distilled/ deionized water and bring volume to 960.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for 1 min. Cool to room temperature under 80% N2 + 20% CO2. Dispense under same gas atmosphere into culture vessels. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add fumarate, sulfide, vitamin, and bicarbonate solutions. Adjust the pH to 7.2.

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Use: For the cultivation of Sulfurospirillum spp.

Use: For the cultivation of Aquabacter spp.

Preparation of Medium: Add components, except vitamin solution, to 980.0mL distilled/deionized water. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 20.0mL sterile vitamin solution. Mix thoroughly. Aseptically distribute to sterile tubes or flasks.

Super HiVeg Broth Composition per liter: Plant hydrolysate......................................................................... 35.0g Yeast extract................................................................................ 20.0g NaCl .............................................................................................. 5.0g pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Escherichia coli.

Super MMB Medium (LMG Medium 188) Yeast extract.................................................................................. 1.0g KH2PO4 ......................................................................................... 1.0g Peptone.......................................................................................... 0.4g Sodium succinate .......................................................................... 0.4g NH4Cl ........................................................................................... 0.2g NaCl .............................................................................................. 0.2g MgSO4·7H2O ................................................................................ 0.2g CaCl2·2H2O.............................................................................. 10.0mg Ferric citrate ............................................................................... 5.0mg Vitamin solution.......................................................................20.0mL Trace elements solution SL-6 ...................................................1.0mL pH 7.0 ± 0.2 at 25°C

Trace Elements Solution SL-6 : Composition per liter: H3BO3 ........................................................................................... 0.3g CoCl2·6H2O .................................................................................. 0.2g ZnSO4·7H2O ................................................................................. 0.1g MnCl2·4H2O................................................................................ 0.03g Na2MoO4·H2O ............................................................................ 0.03g NiCl2·6H2O ................................................................................. 0.02g CuCl2·2H2O................................................................................. 0.01g

Preparation ofTrace Elements Solution SL-6 : Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3.4. Vitamin Solution: Composition per liter: Calcium DL-pantothenate........................................................... 5.0mg Riboflavin .................................................................................. 5.0mg Thiamine·HCl ............................................................................ 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg © 2010 by Taylor and Francis Group, LLC

Superbroth Composition per liter: Pancreatic digest of casein.......................................................... 32.0g Yeast extract................................................................................ 20.0g NaCl.............................................................................................. 5.0g NaOH (1N solution)...................................................................5.0mL

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Escherichia coli. Supplemented (Arginine) M9 Medium See: M9 Medium with Arginine

Supplemented Aspergillus Minimal Agar Composition per liter: Agar ............................................................................................ 20.0g Glucose ....................................................................................... 20.0g NaNO3 .......................................................................................... 6.0g (NH4)2SO4 .................................................................................... 5.0g Casamino acids ............................................................................. 2.0g K2HPO4 ...................................................................................... 1.52g KH2PO4......................................................................................... 1.0g KCl.............................................................................................. 0.52g MgSO4·7H2O.............................................................................. 0.52g NaCl.............................................................................................. 0.1g CaCl2·2H2O .................................................................................. 0.1g Inositol ....................................................................................... 2.0mg KI ............................................................................................... 1.0mg H3BO3 ........................................................................................ 0.5mg ZnSO4·7H2O .............................................................................. 0.4mg MnSO4·4H2O ............................................................................. 0.4mg Thiamine·HCl ............................................................................ 0.4mg Pyroxidine·HCl .......................................................................... 0.4mg Niacin......................................................................................... 0.4mg Calcium pantothenate ................................................................ 0.4mg p-Aminobenzoic acid................................................................. 0.2mg Riboflavin .................................................................................. 0.2mg Na2MoO4·4H2O ......................................................................... 0.2mg CuSO4·5H2O ............................................................................ 0.04mg Folic acid .................................................................................... 2.0μg Biotin .......................................................................................... 2.0μg FeCl3·6H2O................................................................................. 1.0μg ZnSO4·7H2O............................................................................... 1.0μg pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.5. Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-

Sweet E Broth for Anaerobes

clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Aspergillus species.

Supplemented Aspergillus Minimal Broth Composition per liter: Glucose ....................................................................................... 20.0g NaNO3 .......................................................................................... 6.0g (NH4)2SO4 ..................................................................................... 5.0g Casamino acids ............................................................................. 2.0g K2HPO4 ...................................................................................... 1.52g KH2PO4 ......................................................................................... 1.0g KCl.............................................................................................. 0.52g MgSO4·7H2O .............................................................................. 0.52g NaCl .............................................................................................. 0.1g CaCl2·2H2O................................................................................... 0.1g Inositol ....................................................................................... 2.0mg KI ............................................................................................... 1.0mg H3BO3 ........................................................................................ 0.5mg ZnSO4·7H2O .............................................................................. 0.4mg MnSO4·4H2O ............................................................................. 0.4mg Thiamine·HCl ............................................................................ 0.4mg Pyroxidine·HCl .......................................................................... 0.4mg Niacin......................................................................................... 0.4mg Calcium pantothenate ................................................................ 0.4mg p-Aminobenzoic acid................................................................. 0.2mg Riboflavin .................................................................................. 0.2mg Na2MoO4·4H2O ......................................................................... 0.2mg CuSO4·5H2O ............................................................................ 0.04mg Folic acid.....................................................................................2.0μg Biotin ..........................................................................................2.0μg FeCl3·6H2O.................................................................................1.0μg ZnSO4·7H2O ...............................................................................1.0μg pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.5. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation of Aspergillus species. Supplemented (Tryptophan) M9 Medium See: M9 Medium with Tryptophan

SW 2 Agar Composition per liter: Agar ............................................................................................ 15.0g NH4Cl ........................................................................................... 1.0g Sodium acetate ............................................................................ 0.02g Artificial seawater.........................................................................1.0L

Artificial Seawater: Composition per liter: NaCl ............................................................................................ 24.7g MgSO4·7H2O ................................................................................ 6.3g MgCl2·6H2O.................................................................................. 4.6g CaCl2 ............................................................................................. 1.0g KCl................................................................................................ 0.7g NaHCO3 ........................................................................................ 0.2g

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Preparation of Medium: Add solid components to 1.0L of artificial seawater. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation and cultivation of Cytophaga species, Herpetosiphon species, Saprospira species, and Flexithrix species.

SWA See: Seawater Agar

Swampy Medium Composition per liter: Agar ............................................................................................ 10.0g CaCO3 ......................................................................................... 10.0g Peptone ......................................................................................... 0.5g Yeast extract.................................................................................. 0.5g

Preparation of Medium: Add components to seawater and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Vibrio liquefaciens.

Sweet E Broth for Anaerobes Composition per 100.0mL: Gelatin........................................................................................... 0.3g Cellobiose ..................................................................................... 0.1g Fructose......................................................................................... 0.1g Glucose ......................................................................................... 0.1g L-Arabinose ................................................................................... 0.1g Maltose ......................................................................................... 0.1g Starch ............................................................................................ 0.1g Agar .......................................................................................... 0.075g Peptone ....................................................................................... 0.05g L-Cysteine·HCl·H2O ................................................................... 0.05g (NH4)2SO4 .................................................................................. 0.05g Yeast extract................................................................................ 0.05g Salts solution............................................................................50.0mL Rumen fluid .............................................................................30.0mL Resazurin solution .....................................................................0.4mL Pyruvic acid .............................................................................0.01mL pH 6.5 ± 0.2 at 25°C

Preparation of Salts Solution: Add CaCl2 and MgSO4·7H2O to distilled/deionized water and bring volume to 300.0mL. Mix thoroughly. Bring volume to 800.0mL with distilled/deionized water. Add remaining components while stirring. Bring volume to 1.0L with distilled/deionized water. Mix thoroughly. Store at 4°C.

Preparation of Artificial Seawater: Add components to distilled/

Resazurin Solution: Composition per 44.0mL:

deionized water and bring volume to 1.0L. Mix thoroughly.

Resazurin .................................................................................. 0.011g

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SWM Medium

Preparation of Resazurin Solution: Add resazurin to distilled/ deionized water and bring volume to 44.0mL. Mix thoroughly.

p-Aminobenzoic acid............................................................... 80.0mg D(+)-Biotin............................................................................... 20.0mg

Preparation of Medium: Add components to distilled/deionized

Preparation of Solution C (Seven Vitamin Solution): Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Sparge with 100% N2.

water and bring volume to 100.0mL. Mix thoroughly. Gently heat and bring to boiling under O2-free 97% N2 + 3% H2. Adjust the pH to 6.5 if necessary. Continue boiling until the medium turns yellow. Distribute into tubes or flasks under O2-free 97% N2 + 3% H2. Cap tubes with rubber stoppers. Place tubes in a press. Autoclave for 15 min at 15 psi pressure–121°C with fast exhaust.

Use: For the cultivation and maintenance of Clostridium cocleatum and Clostridium spiroforme.

SWM Medium Composition per 1014.0mL: Solution A ..............................................................................940.0mL Solution E (NaHCO3 solution) ................................................50.0mL Solution F (Substrate solution) ................................................10.0mL Solution G (Na2S·9H2O solution) ............................................10.0mL Solution B (Trace elements solution SL-10)..............................2.0mL Solution C (Seven vitamin solution)..........................................1.0mL Solution D (Selenite-tungstate solution)....................................1.0mL pH 7.2–7.4 at 25°C

Solution A: Composition per 940.0mL:

Solution D (Selenite-Tungstate Solution): Composition per liter: NaOH............................................................................................ 0.5g Na2WO4·2H2O........................................................................... 4.0mg Na2SeO3·5H2O........................................................................... 3.0mg

Preparation of Solution D (Selenite-Tungstate Solution): Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Sparge with 100% N2.

Solution E (NaHCO3 Solution): Composition per 50.0mL: NaHCO3 ........................................................................................ 2.5g

Preparation of Solution E (NaHCO3 Solution): Add NaHCO3

to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Gas under 100% N2. Autoclave for 15 min at 15 psi pressure– 121°C.

Solution F (Substrate Solution): Composition per 10.0mL: Pyrogallol...................................................................................... 0.5g

NaCl .............................................................................................. 1.0g KCl................................................................................................ 0.5g MgCl2·6H2O.................................................................................. 0.4g NH4Cl ......................................................................................... 0.25g KH2PO4 ......................................................................................... 0.2g CaCl2·2H2O................................................................................. 0.15g Resazurin ................................................................................... 0.5mg

Preparation of Solution F (Substrate Solution): Add pyrogallol to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Gas under 100% N2. Autoclave for 15 min at 15 psi pressure– 121°C.

Preparation of Solution A: Prepare and dispense under 80% N2 +

Preparation of Solution G (Na2S·9H2O Solution): Add

20% CO2. Add components to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Solution B (Trace Elements Solution SL-10): Composition per liter: FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O........................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution) ..................................................................10.0mL

Preparation of Solution B (Trace Elements Solution SL-10): Prepare and dispense under 100% N2. Add FeCl2·4H2O to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Solution C (Seven Vitamin Solution): Composition per liter: Pyridoxine·HCl ...................................................................... 300.0mg Nicotinic acid ......................................................................... 200.0mg Thiamine·HCl ........................................................................ 200.0mg Calcium pantothenate ............................................................ 100.0mg Cyanocobalamine................................................................... 100.0mg © 2010 by Taylor and Francis Group, LLC

Solution G (Na2S·9H2O Solution): Composition per 10.0mL: Na2S·9H2O.................................................................................... 0.3g Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Gas under 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: To 940.0mL of sterile solution A, aseptically and anaerobically add 1.0mL of sterile solution B, 1.0mL of sterile solution C, 1.0mL of sterile solution D, 50.0mL of sterile solution E, 10.0mL of sterile solution F, and 10.0mL of sterile solution G. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or flasks. Use: For the cultivation and maintenance of Pelobacter massiliensis.

SWMTY Marine Medium Composition per liter: Marine salts mix ......................................................................... 38.0g Agar ............................................................................................ 15.0g Pancreatic digest of casein............................................................ 2.0g Yeast extract.................................................................................. 2.0g Tris(hydroxymethyl)aminomethane buffer................................... 1.0g KNO3 ............................................................................................ 0.5g Sodium glycerophosphate............................................................. 0.1g Trace elements solution HO-LE ................................................1.0mL pH 7.0 ± 0.2 at 25°C

SYFAC Medium

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Sodium tartrate............................................................................ 1.77g FeSO4·7H2O................................................................................ 1.36g CoCl2·6H2O ................................................................................ 0.04g CuCl2·2H2O............................................................................... 0.027g Na2MoO4·2H2O ........................................................................ 0.025g ZnCl2 ........................................................................................... 0.02g

cose solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Preparation of Trace Elements Solution HO-LE: Add compo-

Composition per liter:

nents to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Use: For the isolation and cultivation of Cytophaga species, Herpetosiphon species, Saprospira species, and Flexithrix species.

SYA Medium Agar ............................................................................................ 20.0g Soluble starch.............................................................................. 10.0g Yeast extract.................................................................................. 2.0g

Use: For the cultivation and maintenance of a variety of heterotrophic

marine bacterial species.

Use: For the cultivation and maintenance of Streptomyces chartreusis.

SXT Blood Agar Composition per liter: Pancreatic digest of casein .......................................................... 14.5g Agar ............................................................................................ 14.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 5.0g Growth factor, BBL ...................................................................... 1.5g Sulfamethoxazole...................................................................... 0.024g Trimethoprim ........................................................................... 1.25mg Sheep blood, defibrinated ........................................................50.0mL pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components, except defibrinated sheep blood, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of defibrinated sheep blood. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation of Lancefield group A and group B streptococci from throat cultures and other clinical specimens.

SY Broth Composition per liter: (NH4)2SO4 ..................................................................................... 2.0g Na2HPO4·2H2O............................................................................. 1.4g KH2PO4 ......................................................................................... 0.7g MgSO4·7H2O ................................................................................ 0.2g FeSO4 ......................................................................................... 5.0mg MnSO4 ....................................................................................... 5.0mg Glucose solution ....................................................................100.0mL

Glucose Solution: Composition per 100.0mL: D-Glucose .................................................................................... 10.0g

Preparation of Glucose Solution: Add D-glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Preparation of Medium: Add components, except glucose solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile glu© 2010 by Taylor and Francis Group, LLC

SYC Medium Composition per liter: Sucrose........................................................................................ 10.0g Pancreatic digest of casein............................................................ 8.0g Yeast extract.................................................................................. 4.0g K2HPO4......................................................................................... 3.0g MgSO4·7H2O ................................................................................ 0.3g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Agrobacterium tumefaciens.

SYFAC Medium (DSMZ Medium 1041) Composition per liter: Sea salts, Sigma .......................................................................... 35.0g Yeast extract ................................................................................. 1.0g Resazurin ................................................................................. 0.5 mg Vitamin solution.......................................................................10.0mL Fumarate solution ....................................................................10.0mL Bicarbonate solution ................................................................10.0mL Acetate solution .......................................................................10.0mL Sulfide solution........................................................................10.0mL Wolfe's mineral elixir.................................................................1.0mL pH 7.1 ± 0.1 at 25°C

Sulfide Solution: Composition per 10.0mL: Na2S·9H2O.................................................................................... 0.3g

Preparation of Sulfide Solution: Add Na2S·9H2O to distilled/de-

ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Bicarbonate Solution: Composition per 10.0mL: NaHCO3 ....................................................................................... 2.0g

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 20% CO2 + 80% N2. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to room temperature.

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SYLC Medium

Acetate Solution: Composition per 10.0mL: Na-acetate .................................................................................... 1.6g

Preparation of Acetate Solution: Add Na-acetate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Fumarate Solution: Composition per 10.0mL: Na2-fumarate ................................................................................ 3.2g

Preparation of Fumarate Solution: Add Na2-fumarate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Wolfe’s Mineral Elixir: Composition per liter: MgSO4·7H2O .............................................................................. 30.0g NaCl ............................................................................................ 10.0g MnSO4·2H2O ................................................................................ 5.0g (NH4)2NiSO4·6H2O ...................................................................... 2.8g CoCl2·6H2O .................................................................................. 1.8g ZnSO4·7H2O ................................................................................. 1.8g FeSO4·7H2O.................................................................................. 1.0g CaCl2·2H2O................................................................................... 1.0g KAl(SO4)2·12H2O....................................................................... 0.18g CuSO4·5H2O ................................................................................. 0.1g H3BO3 ........................................................................................... 0.1g Na2MoO4·2H2O ............................................................................ 0.1g Na2SeO4 ........................................................................................ 0.1g Na2WO4·2H2O .............................................................................. 0.1g

Use: For the cultivation of Desulfuromusa ferrireducens.

SYLC Medium (DSMZ Medium 1040) Composition per liter: Sea salts, Sigma .......................................................................... 35.0g Yeast extract ................................................................................. 1.0g Resazurin ................................................................................. 0.5 mg Vitamin solution.......................................................................10.0mL Bicarbonate solution ................................................................10.0mL Lactate solution........................................................................10.0mL Sulfide solution........................................................................10.0mL Wolfe's mineral elixir.................................................................1.0mL pH 7.1 ± 0.1 at 25°C

Sulfide Solution: Composition per 10.0mL: Na2S·9H2O.................................................................................... 0.3g

Preparation of Sulfide Solution: Add Na2S·9H2O to distilled/de-

ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Bicarbonate Solution: Composition per 10.0mL: NaHCO3 ....................................................................................... 2.0g

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 20% CO2 + 80% N2. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to room temperature.

Lactate Solution: Composition per 10.0mL:

Preparation of Wolfe’s Mineral Elixir: Adjust pH of 1.0L of dis-

Na-lactate...................................................................................... 2.5g

tilled/deionized water to 1.0 with dilute H2SO4. Add remaining components one at a time. Mix thoroughly to dissolve.

Preparation of Lactate Solution: Add Na-lactate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Vitamin Solution: Composition per liter: Pyridoxine-HCl ........................................................................ 10.0mg Thiamine-HCl·2H2O .................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg D-Ca-pantothenate...................................................................... 5.0mg p-Aminobenzoic acid ................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize. Preparation of Medium: Add components, except sulfide, acetate, fumarate, bicarbonate, and vitamin solutions, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Filter sterilize. Gently heat and bring to boiling. Boil for 1 min. Cool to room temperature while sparging with 80% N2 + 20% CO2 gas mixture. Dispense under same gas atmosphere in culture vessels. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aspetically add acetate, bicarbonate, fumarate, vitamin, and sulfide solutions from sterile, anoxic solutions. Mix thoroughly. Adjust the pH to 7.0–7.2. © 2010 by Taylor and Francis Group, LLC

Wolfe’s Mineral Elixir: Composition per liter: MgSO4·7H2O .............................................................................. 30.0g NaCl............................................................................................ 10.0g MnSO4·2H2O ................................................................................ 5.0g (NH4)2NiSO4·6H2O ...................................................................... 2.8g CoCl2·6H2O .................................................................................. 1.8g ZnSO4·7H2O ................................................................................. 1.8g FeSO4·7H2O.................................................................................. 1.0g CaCl2·2H2O .................................................................................. 1.0g KAl(SO4)2·12H2O....................................................................... 0.18g CuSO4·5H2O ................................................................................. 0.1g H3BO3 ........................................................................................... 0.1g Na2MoO4·2H2O ............................................................................ 0.1g Na2SeO4 ........................................................................................ 0.1g Na2WO4·2H2O .............................................................................. 0.1g

Preparation of Wolfe’s Mineral Elixir: Adjust pH of 1.0L of distilled/deionized water to 1.0 with dilute H2SO4. Add remaining components one at a time. Mix throughly to dissolve. Vitamin Solution: Composition per liter: Pyridoxine-HCl........................................................................ 10.0mg Thiamine-HCl·2H2O.................................................................. 5.0mg

Synthetic Complete Medium

Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg D-Ca-pantothenate...................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

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Aminoacetic acid ........................................................................ 0.06g L-Cystine ..................................................................................... 0.05g

MgSO4 ........................................................................................ 0.05g L-Proline...................................................................................... 0.05g DL-Tryptophan............................................................................. 0.05g

Nicotinamide............................................................................... 0.01g Thiamine·HCl ............................................................................. 0.01g pH 7.1 ± 0.1 at 25°C

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize.

Source: This medium is available as a premixed powder from BD Di-

Preparation of Medium: Add components, except sulfide, lactate,

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 20 min at 15 psi pressure–121°C.

bicarbonate, and vitamin solutions, to distilled/deionized water and bring volume to 960.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Gently heat and bring to boiling. Boil for 1 min. Cool to room temperature while sparging with 80% N2 + 20% CO2 gas mixture. Dispense under same gas atmosphere in culture vessels. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aspetically add lactate, bicarbonate, vitamin, and sulfide solutions from sterile, anoxic solutions. Mix thoroughly. Adjust the pH to 7.0–7.2.

Use: For the cultivation of Desulfovibrio ferrireducens.

Syncase Broth

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

Use: For the determination of phenol coefficients of disinfectants.

Synthetic Complete Medium Composition per liter: Solution A..............................................................................600.0mL Solution B ..............................................................................400.0mL

Solution A: Composition per 600.0mL:

Composition per liter:

Agar ............................................................................................ 20.0g

Casamino acids ........................................................................... 20.0g K2HPO4 ....................................................................................... 8.71g Yeast extract.................................................................................. 6.0g NaCl .............................................................................................. 2.5g pH 8.5 ± 0.2 at 25°C

Preparation of Solution A: Add agar to distilled/deionized water

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.5. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation of heat-labile, toxin-producing Escherichia coli from foods.

Synthetic Broth, AOAC (Synthetic Broth, Association of Official Analytical Chemists) Composition per liter: Na2HPO4 ....................................................................................... 4.0g NaCl .............................................................................................. 3.0g K2HPO4 ......................................................................................... 1.5g L-Glutamic acid ............................................................................. 1.3g DL-Valine ....................................................................................... 1.0g L-Lysine....................................................................................... 0.85g L-Leucine....................................................................................... 0.8g DL-Serine ..................................................................................... 0.61g DL-Threonine................................................................................. 0.5g L-Aspartic acid ............................................................................ 0.45g DL-Isoleucine............................................................................... 0.44g DL-Alanine................................................................................... 0.43g L-Arginine ..................................................................................... 0.4g DL-Methionine............................................................................. 0.37g DL-Histidine................................................................................... 0.3g DL-Phenylalanine......................................................................... 0.26g L-Tyrosine.................................................................................... 0.21g KCl................................................................................................ 0.2g © 2010 by Taylor and Francis Group, LLC

and bring volume to 600.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45– 50°C.

Solution B: Composition per 400.0mL: Glucose ....................................................................................... 20.0g Yeast nitrogen base without amino acids...................................... 6.7g L-Leucine .................................................................................... 0.18g L-Alanine ................................................................................. 90.0mg L-Arginine................................................................................ 90.0mg L-Asparagine............................................................................ 90.0mg L-Aspartic acid......................................................................... 90.0mg L-Cysteine ................................................................................ 90.0mg L-Glutamine ............................................................................. 90.0mg L-Glutamic acid ....................................................................... 90.0mg Glycine..................................................................................... 90.0mg L-Histidine ............................................................................... 90.0mg i-Inositol................................................................................... 90.0mg L-Isoleucine.............................................................................. 90.0mg L-Lysine ................................................................................... 90.0mg L-Methionine............................................................................ 90.0mg L-Phenylalanine ....................................................................... 90.0mg L-Proline .................................................................................. 90.0mg L-Serine.................................................................................... 90.0mg L-Threonine.............................................................................. 90.0mg L-Tryptophan ........................................................................... 90.0mg L-Tyrosine ................................................................................ 90.0mg L-Valine.................................................................................... 90.0mg Adenine.................................................................................... 23.0mg p-Aminobenzoic acid................................................................. 9.0mg

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly. Filter sterilize. Warm to 45°–50°C prior to preparation of medium.

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Synthetic Malate Medium with 0.25% Sodium Chloride

Preparation of Medium: Aseptically combine 600.0mL of sterile

Preparation of Medium: Add components to distilled/deionized

solution A with 400.0mL of sterile solution B. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Saccharomyces cerevi-

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

siae.

Use: For the cultivation of marine bacteria.

Synthetic Malate Medium with 0.25% Sodium Chloride Composition per 1010.0mL: DL-Malic

acid................................................................................ 5.0g KOH.............................................................................................. 4.5g NaCl .............................................................................................. 2.5g KH2PO4 ......................................................................................... 0.6g NH4Cl ........................................................................................... 0.5g K2HPO4 ......................................................................................... 0.4g MgSO4·7H2O ................................................................................ 0.2g Yeast extract.................................................................................. 0.1g CaCl2 ........................................................................................... 0.02g MnSO4·H2O ................................................................................ 0.01g Na2MoO4·2H2O ........................................................................ 0.002g Biotin ......................................................................................... 0.1mg Ferric EDTA solution...............................................................10.0mL pH 7.2 ± 0.2 at 25°C

Ferric EDTA Solution: Composition per 100.0mL: Ferric EDTA................................................................................ 0.66g

Synthetic Seawater Medium Composition per liter: NaCl............................................................................................ 27.0g MgSO4·7H2O ................................................................................ 7.0g Monosodium glutamate ................................................................ 5.0g Tris(hydroxymethyl)aminomethane buffer................................... 2.0g Glucose ......................................................................................... 1.0g KCl................................................................................................ 0.6g CaCl2 ............................................................................................. 0.3g Sodium glycerophosphate............................................................. 0.2g Vitamin B12 ................................................................................. 1.0μg pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation and maintenance of Leucothrix mucor.

Syntrophobacter pfennigii Medium

Preparation of Ferric EDTA Solution: Add ferric EDTA to dis-

Composition per liter:

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

NaCl.............................................................................................. 1.0g Na2SO4 .......................................................................................... 0.7g KCl................................................................................................ 0.5g MgCl2·6H2O ................................................................................. 0.4g NH4Cl ......................................................................................... 0.25g KH2PO4......................................................................................... 0.2g CaCl2·2H2O ................................................................................ 0.15g Resazurin ................................................................................... 1.0mg Sodium propionate solution.....................................................50.0mL Na2S·9H2O solution .................................................................10.0mL Na2S2O4 solution .....................................................................10.0mL Trace elements solution SL-10 ..................................................1.0mL Seven vitamin solution ..............................................................1.0mL NaHCO3 solution .................................................................... variable pH 7.2–7.4 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation of Azospirillum halopraeferens.

Synthetic Mucor Agar Composition per liter: Glucose ....................................................................................... 40.0g Agar ............................................................................................ 15.0g Asparagine .................................................................................... 2.0g KH2PO4 ......................................................................................... 0.5g MgSO4 ........................................................................................ 0.25g Thiamine·HCl ............................................................................ 0.5mg

Use: For the cultivation and maintenance of Mucor species.

Synthetic Sea Salt Composition per liter: NaCl ............................................................................................ 14.9g MgSO4·7H2O .............................................................................. 3.80g MnCl2·6H2O ............................................................................... 2.94g KCl............................................................................................ 0.435g NaHCO3 .................................................................................. 0.1515g Borax.......................................................................................... 3.0mg SrCl2·6H2O ................................................................................ 0.7mg pH 7.3 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

Sodium Propionate Solution: Composition per 50.0mL: Sodium propionate........................................................................ 1.5g

Preparation of Sodium Propionate Solution: Add sodium propionate to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize. Sparge with 80% N2 + 20% CO2. NaHCO3 Solution: Composition per 20.0mL: NaHCO3 ........................................................................................ 2.5g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize. Sparge with 80% N2 + 20% CO2. Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O.................................................................................. 0.36g

Syntrophobacter wolinii Medium Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Na2S2O4 Solution: Composition per 10.0mL: Na2S2O4·5H2O .............................................................................. 2.0g

Preparation of Na2S2O4 Solution: Add Na2S2O4·5H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Trace Elements Solution SL-10: Composition per liter: FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O........................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution) ..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Prepare and dispense under 80% N2 + 20% CO2. Add FeCl2·4H2O to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C.

Seven Vitamin Solution: Composition per liter: Pyridoxine·HCl ............................................................................. 0.3g Thiamine·HCl ............................................................................... 0.2g Nicotinic acid ................................................................................ 0.2g Calcium DL-pantothenate.............................................................. 0.1g Vitamin B12 ................................................................................... 0.1g p-Aminobenzoic acid............................................................... 80.0mg Biotin ....................................................................................... 20.0mg

Preparation of Seven Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium: Prepare medium and dispense under 80% N2 + 20% CO2. Add components, except sodium propionate solution, NaHCO3 solution, Na2S·9H2O solution, and Na2S2O3 solution, to distilled/deionized water and bring volume to 930.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add 50.0mL of sterile sodium propionate solution, 10.0mL of sterile Na2S2O3 solution, and 10.0mL of sterile Na2S·9H2O solution. Mix thoroughly. Aseptically and anaerobically add sufficient volume of sterile NaHCO3 solution to bring pH to 7.2–7.4. Aseptically and anaerobically distribute into sterile tubes or flasks.

Use: For the cultivation of Syntrophobacter pfennigii.

Syntrophobacter wolinii Medium Solution A ..............................................................................916.0mL Solution B ................................................................................70.0mL Solution C ................................................................................10.0mL Solution D ................................................................................10.0mL pH 7.2 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

1673

Solution A: Composition per 916.0mL: Na2SO4 .......................................................................................... 2.8g Sodium propionate........................................................................ 1.5g Pancreatic digest of casein............................................................ 1.0g Resazurin ................................................................................... 1.0mg Mineral solution.......................................................................50.0mL Rumen fluid, clarified..............................................................50.0mL Vitamin solution.........................................................................5.0mL Trace elements solution SL-10 ..................................................1.0mL

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 916.0mL. Adjust pH to 7.2. Gently heat and bring to boiling. Continue boiling for a few minutes. Allow to cool to room temperature under 80% N2 + 20% CO2. Distribute into bottles under 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure– 121°C.

Mineral Solution: Composition per liter: Nitrilotriacetic acid ..................................................................... 12.5g NaCl.............................................................................................. 1.0g FeCl3·4H2O................................................................................... 0.2g MnCl2·4H2O ................................................................................. 0.1g CaCl2·2H2O .................................................................................. 0.1g ZnCl2 ............................................................................................ 0.1g CuCl2 .......................................................................................... 0.02g Na2SeO3 ...................................................................................... 0.02g CoCl2·6H2O.............................................................................. 0.017g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O.......................................................................... 0.01g

Preparation of Mineral Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Mix thoroughly.

Vitamin Solution: Composition per liter: Biotin ....................................................................................... 0.25mg Nicotinic acid............................................................................. 2.5mg Thiamine·HCl .......................................................................... 1.25mg p-Aminobenzoic acid............................................................... 1.25mg Pantothenic acid....................................................................... 0.62mg Pyridoxine·HCl .......................................................................... 6.2mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly.

Trace Elements Solution SL-10: Composition per liter: FeCl2·4H2O................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O.............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly.

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Syntrophococcus sucromutans Medium

Solution B: Composition per 70.0mL: NaHCO3 ........................................................................................ 3.5g

Preparation of Solution B: Add NaHCO3 to distilled/deionized

p-Aminobenzoic acid............................................................... 1.25mg Thiamine·HCl .......................................................................... 1.25mg Pantothenic acid....................................................................... 0.62mg Biotin ....................................................................................... 0.25mg

water and bring volume to 70.0mL. Mix thoroughly. Filter sterilize. Sparge with 80% N2 + 20% CO2 for 15 min.

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly.

Solution C: Composition per 10.0mL:

Trace Elements Solution SL-10: Composition per liter:

L-Cysteine·HCl.............................................................................. 0.3g

FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Preparation of Solution C: Add L-cysteine·HCl to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2 for 3–4 min. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C.

Solution D: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.3g

Preparation of Solution D: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2 for 3–4 min. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Preparation of Medium: To 916.0mL of sterile solution A, add 70.0mL of sterile solution B, 10.0mL of sterile solution C, and 10.0mL of sterile solution D. Mix thoroughly.

Use: For the cultivation and maintenance of Syntrophobacter wolinii.

Syntrophococcus sucromutans Medium Composition per 1002.0mL: Solution A ..............................................................................916.0mL Solution C ................................................................................50.0mL Solution B ................................................................................25.0mL Solution D ................................................................................10.0mL Solution E ..................................................................................1.0mL pH 7.2–7.4 at 25°C

Solution A: Composition per 916.0mL:

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Solution B: Composition per 25.0mL: Lactose.......................................................................................... 5.0g

Preparation of Solution B: Add lactose to distilled/deionized water and bring volume to 25.0mL. Mix thoroughly. Filter sterilize.

Solution C: Composition per 50.0mL: NaHCO3 ........................................................................................ 2.5g

Preparation of Solution C: Add NaHCO3 to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Solution D: Composition per 10.0mL: L-Cysteine ................................................................................... 0.24g

Sodium formate............................................................................. 0.6g Resazurin ................................................................................... 1.0mg Rumen fluid, clarified ............................................................300.0mL Mineral solution .......................................................................50.0mL Vitamin solution.........................................................................5.0mL Trace elements solution SL-10 ..................................................1.0mL

Preparation of Solution D: Add L-cysteine to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Solution A: Add components to distilled/deionized

Na2S·9H2O............................................................................... 78.0mg

water and bring volume to 916.0mL. Mix thoroughly. Adjust pH to 6.4. Autoclave for 15 min at 15 psi pressure–121°C.

Mineral Solution: Composition per liter: KH2PO4 ....................................................................................... 10.0g NaCl .............................................................................................. 8.0g NH4Cl ........................................................................................... 8.0g MgCl2·6H2O.................................................................................. 6.6g CaCl2·2H2O................................................................................... 1.0g

Preparation of Mineral Solution: Add components to distilled/

Solution E: Composition per 1.0mL: Preparation of Solution E: Add Na2S·9H2O to distilled/deionized water and bring volume to 1.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Prepare and dispense medium under H2free 80% N2 + 20% CO2. Aseptically and anaerobically combine 916.0mL of sterile solution A with 25.0mL of sterile solution B, 50.0mL of sterile solution C, 10.0mL of sterile solution D, and 1.0mL of sterile solution E. Mix thoroughly. Final pH should be 6.4–6.8 Use: For the cultivation and maintenance of Syntrophococcus sucromutans.

deionized water and bring volume to 1.0L. Mix thoroughly.

Syntrophomonas bryantii Medium

Vitamin Solution: Composition per liter:

Composition per 1026.0mL:

Pyridoxine·HCl .......................................................................... 6.2mg Nicotinic acid ............................................................................. 2.5mg

Solution A..............................................................................916.0mL Solution B ................................................................................70.0mL

Syntrophomonas Medium

Solution C ................................................................................10.0mL Solution D ................................................................................10.0mL Sodium laurate solution ...........................................................10.0mL CaCl2·2H2O solution................................................................10.0mL pH 7.2 ± 0.2 at 25°C

Solution A: Composition per 916.0mL: PIPES (piperazine-N,N´-bis [2-ethanesulfonic acid]) buffer ........................................... 15.12g Na2SO4 .......................................................................................... 2.8g Butyric acid................................................................................... 1.7g Pancreatic digest of casein ............................................................ 1.0g Resazurin ................................................................................... 1.0mg Mineral solution .......................................................................50.0mL Rumen fluid, clarified ..............................................................50.0mL Vitamin solution.........................................................................5.0mL Trace elements solution SL-10 ..................................................1.0mL

Mineral Solution: Composition per liter: Nitrilotriacetic acid ..................................................................... 12.5g NaCl .............................................................................................. 1.0g FeCl3·4H2O................................................................................... 0.2g MnCl2·4H2O ................................................................................. 0.1g CaCl2·2H2O .................................................................................. 0.1g ZnCl2 ............................................................................................. 0.1g CuCl2 .......................................................................................... 0.02g Na2SeO3 ...................................................................................... 0.02g CoCl2·6H2O .............................................................................. 0.017g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O.......................................................................... 0.01g

Vitamin Solution: Composition per liter:

1675

water and bring volume to 1.0L. Add remaining components. Mix thoroughly.

Solution B: Composition per 70.0mL: NaHCO3 ........................................................................................ 3.5g

Preparation of Solution B: Add NaHCO3 to distilled/deionized

water and bring volume to 70.0mL. Mix thoroughly. Filter sterilize. Sparge with 80% N2 + 20% CO2 for 15 min.

Solution C: Composition per 10.0mL: L-Cysteine·HCl ............................................................................. 0.3g

Solution D: Composition per 10.0mL: Na2S·9H2O.................................................................................... 0.3g

Preparation of Solution D: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2 for 3–4 min. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Sodium Laurate Solution: Composition per 10.0mL: Sodium laurate ............................................................................ 2.78g

Preparation of Sodium Laurate Solution: Add sodium laurate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure– 121°C.

Pyridoxine·HCl .......................................................................... 6.2mg Nicotinic acid ............................................................................. 2.5mg Thiamine·HCl .......................................................................... 1.25mg p-Aminobenzoic acid............................................................... 1.25mg Pantothenic acid ....................................................................... 0.62mg Biotin ....................................................................................... 0.25mg

CaCl2·2H2O Solution: Composition per 40.0mL:

Preparation of Vitamin Solution: Add components to distilled/

Preparation of Medium: To 916.0mL of sterile solution A, add

deionized water and bring volume to 1.0L. Mix thoroughly.

CaCl2·2H2O ................................................................................ 1.84g

Preparation of CaCl2·2H2O Solution: Add CaCl2·2H2O to dis-

tilled/deionized water and bring volume to 40.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

70.0mL of sterile solution B, 10.0mL of sterile solution C, and 10.0mL of sterile solution D. Mix thoroughly. Prior to inoculation, aseptically add 10.0mL of sterile sodium laurate solution and 10.0mL of sterile CaCl2·2H2O solution. Mix thoroughly.

Use: For the cultivation and maintenance of Syntrophomonas sapovorans.

Syntrophomonas Medium Composition per 1006.0mL: Solution A..............................................................................916.0mL Solution B ................................................................................70.0mL Solution C ................................................................................10.0mL Solution D................................................................................10.0mL pH 7.2 ± 0.2 at 25°C

1676

Syntrophomonas Medium, Sulfate-Free

Solution A: Composition per 916.0mL:

Solution B: Composition per 70.0mL:

Na2SO4 .......................................................................................... 2.8g Butyric acid................................................................................... 1.7g Pancreatic digest of casein ............................................................ 1.0g Resazurin ................................................................................... 1.0mg Mineral solution .......................................................................50.0mL Rumen fluid, clarified ..............................................................50.0mL Vitamin solution.........................................................................5.0mL Trace elements solution SL-10 ..................................................1.0mL

NaHCO3 ........................................................................................ 3.5g

Mineral Solution: Composition per liter:

Preparation of Solution C: Add L-cysteine·HCl to distilled/deion-

Nitrilotriacetic acid ..................................................................... 12.5g NaCl .............................................................................................. 1.0g FeCl3·4H2O................................................................................... 0.2g MnCl2·4H2O ................................................................................. 0.1g CaCl2·2H2O .................................................................................. 0.1g ZnCl2 ............................................................................................. 0.1g CuCl2 .......................................................................................... 0.02g Na2SeO3 ...................................................................................... 0.02g CoCl2·6H2O .............................................................................. 0.017g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O.......................................................................... 0.01g

Vitamin Solution: Composition per liter: Pyridoxine·HCl .......................................................................... 6.2mg Nicotinic acid ............................................................................. 2.5mg Thiamine·HCl .......................................................................... 1.25mg p-Aminobenzoic acid ............................................................... 1.25mg Pantothenic acid ....................................................................... 0.62mg Biotin ....................................................................................... 0.25mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly.

Preparation of Solution B: Add NaHCO3 to distilled/deionized

water and bring volume to 70.0mL. Mix thoroughly. Filter sterilize. Sparge with 80% N2 + 20% CO2 for 15 min.

Solution C: Composition per 10.0mL: L-Cysteine·HCl ............................................................................. 0.3g

ized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2 for 3–4 min. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C.

Solution D: Composition per 10.0mL: Na2S·9H2O.................................................................................... 0.3g

Use: For the cultivation of Syntrophomonas species.

Syntrophomonas Medium, Sulfate-Free Composition per 1006.0mL: Solution A..............................................................................916.0mL Solution B ................................................................................70.0mL Solution C ................................................................................10.0mL Solution D................................................................................10.0mL pH 7.2 ± 0.2 at 25°C

Solution A: Composition per 916.0mL: Butyric acid................................................................................... 1.7g Pancreatic digest of casein............................................................ 1.0g Resazurin ................................................................................... 1.0mg Mineral solution.......................................................................50.0mL Rumen fluid, clarified..............................................................50.0mL Vitamin solution.........................................................................5.0mL Trace elements solution SL-10 ..................................................1.0mL

Preparation of Mineral Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add

Syntrophomonas species Medium

remaining components. Add distilled/deionized water to 1.0L. Mix thoroughly.

Vitamin Solution: Composition per liter: Pyridoxine·HCl .......................................................................... 6.2mg Nicotinic acid ............................................................................. 2.5mg Thiamine·HCl .......................................................................... 1.25mg p-Aminobenzoic acid............................................................... 1.25mg Pantothenic acid ....................................................................... 0.62mg Biotin ....................................................................................... 0.25mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly.

1677

Syntrophomonas species Medium Composition per 1006.0mL: Solution A..............................................................................916.0mL Solution B ................................................................................70.0mL Solution C ................................................................................10.0mL Solution D................................................................................10.0mL pH 7.2 ± 0.2 at 25°C

Solution A: Composition per 916.0mL: Na2SO4 .......................................................................................... 2.8g Pancreatic digest of casein............................................................ 1.0g Sodium stearate........................................................................... 0.61g Resazurin ................................................................................... 1.0mg Mineral solution.......................................................................50.0mL Rumen fluid, clarified..............................................................50.0mL Vitamin solution.........................................................................5.0mL Trace elements solution SL-10 ..................................................1.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

Mineral Solution: Composition per liter:

Nitrilotriacetic acid ..................................................................... 12.5g NaCl.............................................................................................. 1.0g FeCl3·4H2O................................................................................... 0.2g MnCl2·4H2O ................................................................................. 0.1g CaCl2·2H2O .................................................................................. 0.1g ZnCl2 ............................................................................................ 0.1g CuCl2 .......................................................................................... 0.02g Na2SeO3 ...................................................................................... 0.02g CoCl2·6H2O.............................................................................. 0.017g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O.......................................................................... 0.01g

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly.

Solution B: Composition per 70.0mL: NaHCO3 ........................................................................................ 3.5g

Preparation of Solution B: Add NaHCO3 to distilled/deionized

Preparation of Mineral Solution: Add nitrilotriacetic acid to

water and bring volume to 70.0mL. Mix thoroughly. Filter sterilize. Sparge with 80% N2 + 20% CO2 for 15 min.

500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Mix thoroughly.

Solution C: Composition per 10.0mL:

Vitamin Solution: Composition per liter:

L-Cysteine·HCl.............................................................................. 0.3g

Pyridoxine·HCl .......................................................................... 6.2mg Nicotinic acid............................................................................. 2.5mg Thiamine·HCl .......................................................................... 1.25mg p-Aminobenzoic acid............................................................... 1.25mg Pantothenic acid....................................................................... 0.62mg Biotin ....................................................................................... 0.25mg

Solution D: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.3g

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly.

Trace Elements Solution SL-10: Composition per liter: FeCl2·4H2O................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O.............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg

1678

Syntrophothermus Medium

CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution) ..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly.

Solution B: Composition per 70.0mL: NaHCO3 ........................................................................................ 3.5g

Preparation of Solution B: Add NaHCO3 to distilled/deionized water and bring volume to 70.0mL. Mix thoroughly. Filter sterilize. Sparge with 80% N2 + 20% CO2 for 15 min.

Solution C: Composition per 10.0mL: L-Cysteine·HCl.............................................................................. 0.3g

Solution D: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.3g

Use: For the cultivation of Syntrophomonas wolfei.

Syntrophothermus Medium (DSMZ Medium 870) Composition per liter: NaHCO3 ........................................................................................ 2.5g NH4Cl ......................................................................................... 0.54g MgCl2·6H2O.................................................................................. 0.2g CaCl2·2H2O................................................................................. 0.15g KH2PO4 ....................................................................................... 0.14g Resazurin ................................................................................... 0.5mg Na2S·9H2O solution .................................................................10.0mL Cysteine solution......................................................................10.0mL Vitamin solution.......................................................................10.0mL Substrate solution.....................................................................10.0mL Trace elements solution .............................................................1.0mL Selenite-tungstate solution .........................................................1.0mL pH 7.0 ± 0.2 at 25°C

Substrate Solution: Composition per 10.0mL: Na-crotonate................................................................................ 0.86g

Preparation of Substrate Solution: AddNa-crotonate to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature. Cysteine Solution: Composition per 10.0mL: L-Cysteine·HCl·H2O ..................................................................... 0.3g

Preparation of Selenite-Tungstate Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Vitamin Solution: Composition per liter: Pyridoxine-HCl........................................................................ 10.0mg Thiamine-HCl·2H2O.................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg D-Ca-pantothenate ..................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

+ 20% CO2 gas atmosphere. Add components, except cysteine solution, Na2S·9H2O solution, and substrate solution, to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Adjust pH to 7.0. Sparge with

Syntrophus buswellii II Medium

80% N2 + 20% CO2 for 30 min. Distribute into Hungate tubes or serum bottles. Autoclave for 15 min at 15 psi pressure–121°C. For each 10.0mL medium, aseptically and anaerobically add 0.1mL cysteine solution, 0.1mL Na2S·9H2O solution, and 0.1mL substrate solution. Mix thoroughly.

Use: For the cultivation of Syntrophothermus lipocalidus DSM 12680.

Syntrophothermus Medium (DSMZ Medium 870) Composition per liter: NaHCO3 ........................................................................................ 2.5g NH4Cl ......................................................................................... 0.54g MgCl2·6H2O.................................................................................. 0.2g CaCl2·2H2O................................................................................. 0.15g KH2PO4 ....................................................................................... 0.14g Resazurin ................................................................................... 0.5mg Na2S·9H2O solution .................................................................10.0mL Cysteine solution......................................................................10.0mL Vitamin solution.......................................................................10.0mL Substrate solution.....................................................................10.0mL Trace elements solution .............................................................1.0mL Selenite-tungstate solution.........................................................1.0mL pH 7.0 ± 0.2 at 25°C

1679

ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Na-butyrate ................................................................................... 2.2g

Pyridoxine-HCl........................................................................ 10.0mg Thiamine-HCl·2H2O.................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg D-Ca-pantothenate ..................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Substrate Solution: Add Na-butyrate to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize.

Na2S·9H2O Solution: Composition per 10.0mL:

Preparation of Medium: Prepare and dispense medium under 80%

Substrate Solution: Composition per 10.0mL:

Na2S·9H2O .................................................................................... 0.3g

Preparation of Cysteine Solution: Add L-cysteine·HCl·H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure– 121°C. Selenite-Tungstate Solution Composition per liter: NaOH ............................................................................................ 0.5g Na2WO4·2H2O ........................................................................... 4.0mg Na2SeO3·5H2O........................................................................... 3.0mg

N2 + 20% CO2 gas atmosphere. Add components, except cysteine solution, Na2S·9H2O solution, and substrate solution, to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Adjust pH to 7.0. Sparge with 80% N2 + 20% CO2 for 30 min. Distribute into Hungate tubes or serum bottles. Autoclave for 15 min at 15 psi pressure–121°C. For each 10.0mL medium, aseptically and anaerobically add 0.1mL cysteine solution, 0.1mL Na2S·9H2O solution, and 0.1mL substrate solution. Mix thoroughly.

Use: For the cultivation of Syntrophothermus lipocalidus DSM 12681.

Syntrophus buswellii II Medium Composition per 1001.0mL: Solution A..............................................................................870.0mL Solution C ..............................................................................100.0mL Solution D................................................................................10.0mL Solution E (Vitamin solution)..................................................10.0mL Solution F.................................................................................10.0mL Solution B (Trace elements solution SL-10) .............................1.0mL pH 7.1–7.4 at 25°C

Solution A: Composition per 870.0mL: Na2SO4 .......................................................................................... 3.0g NaCl.............................................................................................. 1.0g KCl................................................................................................ 0.5g MgCl2·6H2O ................................................................................. 0.4g NH4Cl ........................................................................................... 0.3g KH2PO4......................................................................................... 0.2g CaCl2·2H2O ................................................................................ 0.15g Resazurin ................................................................................... 1.0mg

1680

Syntrophus Medium

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 870.0mL. Mix thoroughly. Gently heat and bring to boiling. Continue boiling for 3-4 min. Allow to cool to room temperature while gassing under 80% N2 + 20% CO2. Continue gassing until pH reaches below 6.0. Seal the flask under 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Aseptically and anaerobically combine

Solution B (Trace Elements Solution SL-10): Composition per liter:

Use: For the cultivation and maintenance of Syntrophus buswelii.

FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O........................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution) ..................................................................10.0mL

Preparation of Solution B (Trace Elements Solution SL-10): Add FeCl2·4H2O to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Gas under 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Solution C: Composition per 100.0mL: NaHCO3 ........................................................................................ 5.0g

Preparation of Solution C: Add NaHCO3 to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Gas under 80% N2 + 20% CO2.

Solution D: Composition per 10.0mL: Sodium benzoate........................................................................... 3.0g Sodium acetate .............................................................................. 1.0g

Preparation of Solution D: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Gas under 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Solution E (Vitamin Solution): Composition per liter: Pyridoxine·HCl ........................................................................ 10.0mg Calcium DL-pantothenate........................................................... 5.0mg Lipoic acid ................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg p-Aminobenzoic acid ................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Thiamine·HCl ............................................................................ 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Solution E (Vitamin Solution): Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gas under 100% N2. Autoclave for 15 min at 15 psi pressure– 121°C.

Solution F: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.4g

Preparation of Solution F: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Gas under 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. © 2010 by Taylor and Francis Group, LLC

870.0mL of sterile solution A with 1.0mL of sterile solution B, 100.0mL of sterile solution C, 10.0mL of sterile solution D, 10.0mL of sterile solution E, and 10.0mL of sterile solution F, in that order. Mix thoroughly. Anaerobically distribute into sterile tubes or flasks under 100% N2.

Syntrophus Medium Composition per 1006.0mL: Solution A..............................................................................916.0mL Solution B ................................................................................70.0mL Solution C ................................................................................10.0mL Solution D................................................................................10.0mL pH 7.2 ± 0.2 at 25°C

Solution A: Composition per 916.0mL: Na2SO4 .......................................................................................... 2.8g Sodium benzoate........................................................................... 2.0g Pancreatic digest of casein............................................................ 1.0g Resazurin ................................................................................... 1.0mg Mineral solution.......................................................................50.0mL Rumen fluid, clarified..............................................................50.0mL Vitamin solution.........................................................................5.0mL Trace elements solution SL-10 ..................................................1.0mL

Vitamin Solution: Composition per liter: Pyridoxine·HCl .......................................................................... 6.2mg Nicotinic acid............................................................................. 2.5mg Thiamine·HCl .......................................................................... 1.25mg p-Aminobenzoic acid............................................................... 1.25mg Pantothenic acid....................................................................... 0.62mg Biotin ....................................................................................... 0.25mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly.

Syntrophus Medium, Sulfate-Free

NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution) ..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly.

1681

Preparation of Solution B: Add NaHCO3 to distilled/deionized

Vitamin Solution: Composition per liter:

Solution C: Composition per 10.0mL:

Pyridoxine·HCl .......................................................................... 6.2mg Nicotinic acid............................................................................. 2.5mg Thiamine·HCl .......................................................................... 1.25mg p-Aminobenzoic acid............................................................... 1.25mg Pantothenic acid....................................................................... 0.62mg Biotin ....................................................................................... 0.25mg

water and bring volume to 70.0mL. Mix thoroughly. Filter sterilize. Sparge with 80% N2 + 20% CO2 for 15 min.

L-Cysteine·HCl.............................................................................. 0.3g

Solution D: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.3g

Use: For the cultivation and maintenance of Syntrophus buswelii.

Syntrophus Medium, Sulfate-Free

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly.

Trace Elements Solution SL-10: Composition per liter: FeCl2·4H2O................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O.............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly.

Composition per 1006.0mL:

Preparation of Solution A: Add components to distilled/deionized

Solution A ..............................................................................916.0mL Solution B ................................................................................70.0mL Solution C ................................................................................10.0mL Solution D ................................................................................10.0mL pH 7.2 ± 0.2 at 25°C

water and bring volume to 916.0mL. Adjust pH to 7.2. Gently heat and bring to boiling. Continue boiling for a few minutes. Allow to cool to room temperature under 80% N2 + 20% CO2. Distribute into bottles under 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure– 121°C.

Solution A: Composition per 916.0mL: Sodium benzoate........................................................................... 2.0g Pancreatic digest of casein ............................................................ 1.0g Resazurin ................................................................................... 1.0mg Mineral solution .......................................................................50.0mL Rumen fluid, clarified ..............................................................50.0mL Vitamin solution.........................................................................5.0mL Trace elements solution SL-10 ..................................................1.0mL © 2010 by Taylor and Francis Group, LLC

Solution B: Composition per 70.0mL: NaHCO3 ........................................................................................ 3.5g

Preparation of Solution B: Add NaHCO3 to distilled/deionized

water and bring volume to 70.0mL. Mix thoroughly. Filter sterilize. Sparge with 80% N2 + 20% CO2 for 15 min.

Solution C: Composition per 10.0mL: L-Cysteine·HCl ............................................................................. 0.3g

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SYPC Medium

Solution D: Composition per 10.0mL:

Peptic digest of animal tissue ....................................................... 2.5g Sodium heptadecyl sulfate............................................................ 0.1g Bromthymol Blue ......................................................................... 0.1g Bromcresol Purple ........................................................................ 0.1g pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di-

Na2S·9H2O .................................................................................... 0.3g

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

Preparation of Medium: To 916.0mL of sterile solution A, add 70.0mL of sterile solution B, 10.0mL of sterile solution C, and 10.0mL of sterile solution D. Mix thoroughly.

Use: For the cultivation and maintenance of Syntrophus buswelii.

SYPC Medium (DSMZ Medium 1188) Composition per liter: Sea salts, Sigma .......................................................................... 35.0g Yeast extract ................................................................................. 1.0g Trypticase polypeptone ................................................................. 0.5g Lactate solution........................................................................10.0mL Wolfe's mineral elixir.................................................................1.0mL pH 7.5 ± 0.2 at 25°C

Cellobiose Solution: Composition per 10.0mL: Cellobiose ..................................................................................... 2.0g

Preparation of Cellobiose Solution: Add cellobiose to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Wolfe’s Mineral Elixir: Composition per liter: MgSO4·7H2O .............................................................................. 30.0g NaCl ............................................................................................ 10.0g MnSO4·2H2O ................................................................................ 5.0g (NH4)2NiSO4·6H2O ...................................................................... 2.8g CoCl2·6H2O .................................................................................. 1.8g ZnSO4·7H2O ................................................................................. 1.8g FeSO4·7H2O.................................................................................. 1.0g CaCl2·2H2O................................................................................... 1.0g KAl(SO4)2·12H2O....................................................................... 0.18g CuSO4·5H2O ................................................................................. 0.1g H3BO3 ........................................................................................... 0.1g Na2MoO4·2H2O ............................................................................ 0.1g Na2SeO4 ........................................................................................ 0.1g Na2WO4·2H2O .............................................................................. 0.1g

water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. The medium may be made more selective by adding 1.0mg of penicillin G per liter. Pour into sterile Petri dishes or leave in tubes.

Use: For the selective recovery and differential identification of injured coliform microorganisms from chlorinated water by the membrane filter method. For rapid estimation of the bacteriological quality of water using the membrane filter method.

T-ASW Medium Composition per 1003.0mL: NaCl............................................................................................ 25.0g Na2S2O3·5H2O .............................................................................. 2.5g MgSO4·7H2O ................................................................................ 1.5g (NH4)2SO4 .................................................................................... 1.0g KH2PO4......................................................................................... 0.4g CaCl2·2H2O .................................................................................. 0.3g NaHCO3 ........................................................................................ 0.2g Tris·HCl buffer, 0.1M, pH 7.5................................................200.0mL Phenol Red (0.5% solution).......................................................2.0mL Trace elements solution .............................................................1.0mL pH 7.5 ± 0.2 at 25°C

Trace Elements Solution: Composition per liter: Disodium EDTA ......................................................................... 50.0g CaCl2·2H2O .................................................................................. 5.5g MnCl2·4H2O ................................................................................. 5.1g FeSO4·7H2O.................................................................................. 5.0g ZnSO4·7H2O ................................................................................. 2.2g CoCl2·6H2O .................................................................................. 1.6g CuSO4·5H2O ................................................................................. 1.6g (NH4)6Mo7O24·4H2O .................................................................... 1.1g

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0 with KOH. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5. Filter sterilize.

Use: For the cultivation and maintenance of Thiobacillus hydrothermalis.

T 7Agar Base (m-T7 Agar Base)

T2 Medium for Thiobacillus

Composition per liter:

Solution A..............................................................................250.0mL Solution B ..............................................................................250.0mL Solution C ..............................................................................250.0mL Solution D..............................................................................250.0mL pH 7.0 ± 0.2 at 25°C

TAT Broth Base

Solution A: Composition per 250.0mL: Na2S2O3·5H2O .............................................................................. 5.0g KNO3 ............................................................................................ 2.0g NH4Cl ........................................................................................... 1.0g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 250.0mL. Mix thoroughly. Filter sterilize. Solution B: Composition per 250.0mL

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Preparation of Medium: Add agar to 1.0L of tap water. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes. Use: For the cultivation and differentiation of fungi and aerobic actinomycetes based on filament and aerial hyphae morphology.

Tarshis Blood Agar Composition per 1050.0mL:

Solution C: Composition per 250.0mL

Beef heart infusion.................................................................... 500.0g Agar ............................................................................................ 15.0g Meat peptone .............................................................................. 10.0g NaCl.............................................................................................. 5.0g Penicillin G, sterile............................................................... 100,000U Sheep blood, sterile................................................................300.0mL Glycerol ...................................................................................10.0mL pH 6.6 ± 0.2 at 25°C

NaHCO3 ........................................................................................ 2.0g

Preparation of Medium: Add components, except sheep blood and

Preparation of Solution C: Add NaHCO3 to distilled/deionized

penicillin G, to distilled/deionized water and bring volume to 750.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile sheep blood and sterile penicillin G. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

KH2PO4 ......................................................................................... 2.0g

Preparation of Solution B: Add KH2PO4 to distilled/deionized water and bring volume to 250.0mL. Mix thoroughly. Filter sterilize.

water and bring volume to 250.0mL. Mix thoroughly. Filter sterilize.

Solution D: Composition per 250.0mL MgSO4·7H2O ................................................................................ 0.8g FeSO4·7H2O (2%, w/v, in 1N HCl)............................................1.0mL Trace metal solution...................................................................1.0mL

Preparation of Solution D: Add components to distilled/deionized water and bring volume to 250.0mL. Mix thoroughly. Filter sterilize.

FeSO4·7H2O Solution: Composition per 100.0mL FeSO4·7H2O.................................................................................. 2.0g HCl (1N solution)...................................................................100.0mL

Preparation of FeSO4·7H2O Solution: Add the FeSO4·7H2O to the HCl solution. Mix thoroughly.

Trace Metals Solution: Composition per liter:

Use: For the isolation and cultivation of Mycobacterium tuberculosis.

Tartoff-Hobbs HiVeg Broth with Glycerol (Terrific HiVeg Broth) Composition per liter: Yeast extract................................................................................ 24.0g Plant hydrolysate ........................................................................ 12.0g KH2PO4......................................................................................... 9.4g K2HPO4......................................................................................... 2.2g Glycerol .....................................................................................4.0mL pH 7.2 ± 0.2 at 25°C

Source: This medium, without glycerol, is available as a premixed powder from HiMedia.

EDTA .......................................................................................... 50.0g ZnSO4 ......................................................................................... 22.0g CaCl2 ........................................................................................... 5.54g MnCl2 .......................................................................................... 5.06g FeSO4·7H2O................................................................................ 4.99g CoCl2........................................................................................... 1.61g CuSO4 ......................................................................................... 1.57g (NH4)2MoO4 ................................................................................. 1.1g

Preparation of Medium: Add components to distilled/deionized

Preparation of Trace Metals Solution: Add components to dis-

Pancreatic digest of casein.......................................................... 20.0g Lecithin ......................................................................................... 5.0g Polysorbate 20 (Tween™ 20) ..................................................40.0mL pH 7.2 ± 0.2 at 25°C

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0 with KOH.

Preparation of Medium: Aseptically combine the four sterile solu-

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of plasmid-bearing strains of Escherichia coli.

TAT Broth Base (Trypticase™ Azolectin Tween™ Broth Base) Composition per liter:

tions: solution A, solution B, solution C, and solution D. Adjust the pH to 7.0. Aseptically distribute into sterile tubes or flasks.

Source: This medium is available as a premixed powder from BD Di-

Use: For the cultivation and maintenance of Thiobacillus denitrificans and other thiobacilli.

Preparation of Medium: Add pancreatic digest of casein and leci-

Tap Water Agar Composition per liter: Agar ............................................................................................ 15.0g Tap water.......................................................................................1.0L © 2010 by Taylor and Francis Group, LLC

agnostic Systems. thin to distilled/deionized water and bring volume to 960.0mL. Add the Tween™ 20. Mix thoroughly. Gently heat and bring to 48°–50°C for 30 min. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the isolation of Gram-negative organisms from topical drugs and cosmetics.

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TAT HiVeg Broth Base with Polysorbate

TB Broth Base without Polysorbate 80

Composition per liter:

Plant hydrolysate......................................................................... 20.0g Azolectin ....................................................................................... 5.0g Polysorbate 20 (Tween™ 20) ..................................................40.0mL pH 7.2 ± 0.2 at 25°C

Proteose peptone........................................................................... 4.0g Na2HPO4 ....................................................................................... 2.5g Yeast extract.................................................................................. 2.0g Sodium citrate............................................................................... 1.5g KH2PO4......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.6g Bovine albumin solution..........................................................50.0mL Glucose solution ......................................................................10.0mL pH 7.0 ± 0.2 at 25°C

Source: This medium, without polysorbate, is available as a premixed powder from HiMedia.

Preparation of Medium: Add plant hydrolysate and azolectin to distilled/deionized water and bring volume to 960.0mL. Add 40.0mL Tween™ 20. Mix thoroughly. Gently heat and bring to 48°–50°C for 30 min. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the isolation of Gram-negative organisms from topical drugs and cosmetics. Tatum Motility Test and Maintenance Medium See: Motility Test and Maintenance Medium, Tatum Taurocholate Tellurite Gelatin Agar See: Monsur Agar

TB Broth Base Composition per liter:

Source: This medium is available from HiMedia. Glucose Solution: Composition per 10.0mL: Glucose ......................................................................................... 5.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Bovine Albumin Solution: Composition per 50.0mL: Bovine serum albumin.................................................................. 5.0g

Preparation of Bovine Albumin Solution: Add bovine serum albumin to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.

Proteose peptone ........................................................................... 4.0g Na2HPO4 ....................................................................................... 2.5g Yeast extract.................................................................................. 2.0g Sodium citrate ............................................................................... 1.5g KH2PO4 ......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.6g Polysorbate 80............................................................................... 0.5g Bovine albumin solution ..........................................................50.0mL Glucose solution ......................................................................10.0mL Glycerol ....................................................................................5.0mL pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add glycerol to distilled/deionized water

Source: This medium is available from HiMedia.

Plant peptone No. 3....................................................................... 4.0g Na2HPO4 ....................................................................................... 2.5g Yeast extract.................................................................................. 2.0g Sodium citrate............................................................................... 1.5g KH2PO4......................................................................................... 1.0g MgSO4 .......................................................................................... 0.6g Polysorbate 80 .............................................................................. 0.5g Glucose solution ......................................................................50.0mL Bovine albumin solution..........................................................50.0mL pH 7.0 ± 0.2 at 25°C

Glucose Solution: Composition per 10.0mL: Glucose ......................................................................................... 5.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Bovine Albumin Solution: Composition per 50.0mL: Bovine serum albumin .................................................................. 5.0g

Preparation of Bovine Albumin Solution: Add bovine serum al-

and bring volume to 955.0mL. Mix thoroughly. Add remaining components, except bovine albumin and glucose solutions, Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add glucose solution and bovine albumin solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Mycobacterium tuberculosis.

TB HiVeg Broth Base with Bovine Albumin and Glucose Composition per liter:

Source: This medium, without glucose or bovine albumin, is available as a premixed powder from HiMedia.

buin to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.

Glucose Solution: Composition per 100.0mL:

Preparation of Medium: Add components, except bovine albumin

Glucose ....................................................................................... 10.0g

and glucose solution, to distilled/deionized water and bring volume to 960.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 50°C. Aseptically add glucose solution and bovine albumin solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Use: For the cultivation of Mycobacterium tuberculosis.

Bovine albumin fraction V ......................................................... 10.0g

Bovine Albumin Solution: Composition per 100.0mL:

TB Nitrate Reduction Broth Preparation of Bovine Albumin Solution: Add bovine albumin to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 7.8 with NaOH. Store at −20°C.

Preparation of Medium: Add components, except bovine albumin solution and glucose solution, to distilled/deionized water and bring volume to 900.0L. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Add 50.0mL sterile glucose solution and 50.0mL bovine albumin solution. Mix thoroughly. Aseptically distribute into sterile tubes. Use: For the cultivation of Mycobacterium tuberculosis.

TB HiVeg Broth Base with Bovine Serum and Glucose Composition per liter: Plant peptone No. 3....................................................................... 4.0g Na2HPO4 ....................................................................................... 2.5g Yeast extract.................................................................................. 2.0g Sodium citrate ............................................................................... 1.5g KH2PO4 ......................................................................................... 1.0g MgSO4 .......................................................................................... 0.6g Polysorbate 80............................................................................... 0.5g Glucose solution ......................................................................50.0mL Bovine serum ...........................................................................50.0mL pH 7.0 ± 0.2 at 25°C

Source: This medium, without glucose or bovine albumin, is available as a premixed powder from HiMedia. Glucose Solution: Composition per 100.0mL: Glucose ....................................................................................... 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except bovine serum and glucose solution, to distilled/deionized water and bring volume to 900.0L. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Add 50.0mL sterile glucose solution and 50.0mL bovine serum. Mix thoroughly. Aseptically distribute into sterile tubes. Use: For the cultivation of Mycobacterium tuberculosis.

TB HiVeg Broth Base without Tween™ 80 with Bovine Albumin and Glucose Composition per liter: Plant peptone No. 3....................................................................... 4.0g Na2HPO4 ....................................................................................... 2.5g Yeast extract.................................................................................. 2.0g Sodium citrate ............................................................................... 1.5g KH2PO4 ......................................................................................... 1.0g MgSO4 .......................................................................................... 0.6g Glucose solution ......................................................................50.0mL Bovine albumin solution ..........................................................50.0mL pH 7.0 ± 0.2 at 25°C

1685

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Bovine Albumin Solution: Composition per 100.0mL: Bovine albumin fraction V ......................................................... 10.0g

Preparation of Bovine Albumin Solution: Add bovine albumin to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 7.8 with NaOH. Store at −20°C.

Use: For the cultivation of mycobacteria when the presence of oleic acid is undesirable. For the cultivation of Mycobacterium tuberculosis.

TB HiVeg Broth Base without Tween™ 80 with Bovine Serum and Glucose Composition per liter: Plant peptone No. 3....................................................................... 4.0g Na2HPO4 ....................................................................................... 2.5g Yeast extract.................................................................................. 2.0g Sodium citrate............................................................................... 1.5g KH2PO4......................................................................................... 1.0g MgSO4 .......................................................................................... 0.6g Glucose solution ......................................................................50.0mL Bovine serum ...........................................................................50.0mL pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Glucose Solution: Composition per 100.0mL: Glucose ....................................................................................... 10.0g

TB Nitrate Reduction Broth Composition per 100.0mL: Na2HPO4·12H2O....................................................................... 0.485g KH2PO4..................................................................................... 0.117g NaNO3 ...................................................................................... 0.085g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

1686

TBAB 298 Medium

Use: For the differentiation of Mycobacterium species based on nitrate reduction. After growth of cells in appropriate medium, nitrate reduction is determined by making a suspension of cells in TB nitrate reduction broth and adding hydrochloric acid, sulfanilamide, and N-naphylenendiamine. Nitrate reduction turns the medium pink. Mycobacterium tuberculosis reduces nitrate and turns the medium deep pink within 1 min. Mycobacterium bovis does not reduce nitrate and does not change the medium.

TBAB 298 Medium (Tryptose 298 Blood Agar Base Medium) Tryptose Blood Agar Base: Composition per 409.6mL: Agar ............................................................................................ 15.0g Tryptose ...................................................................................... 10.0g NaCl .............................................................................................. 5.0g Beef extract ................................................................................... 3.0g Glucose solution ........................................................................4.0mL Thymine solution .......................................................................2.0mL D-Alanine solution......................................................................2.0mL Streptomycin sulfate solution ....................................................1.6mL pH 7.2 ± 0.2 at 25°C

Glucose Solution: Composition per 100.0mL:

TBX Agar (Tryptone Bile X-glucuronide Agar) Composition per liter: Peptone ....................................................................................... 20.0g Agar ............................................................................................ 15.0g Bile salts........................................................................................ 1.5g X-β-D-glucuronide.................................................................... 0.075g pH 7.2 ± 0.2 at 25°C

Source: This medium is available from Fluka, Sigma-Aldrich. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes.

Use: For the detection and enumeration of E. coli in foodstuffs, animal food, and water without further confirmation. E. coli colonies are colored blue-green. The presence of the enzyme β-D-glucuronidase differentiates most E. coli sp. from other coliforms. E. coli absorbs the chromogenic substrate 5-bromo-4-chloro-3-indolyl-β-D-glucuronide. The enzyme β-glucuronidase splits the bond between the chromophore 5bromo-4-chloro-3-indolyl and the β-D-glucuronide. Growth of accompanying Gram-positive flora is largely inhibited by the use of bile salts.

TBYA Agar (Tryptone Beef Yeast Extract Acetate Agar)

Glucose ....................................................................................... 50.0g

Composition per liter:

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Thymine Solution: Composition per 10.0mL: Thymine ........................................................................................ 0.1g

Preparation of Thymine Solution: Add thymine to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. D-Alanine

Solution: Composition per 10.0mL:

D-Alanine....................................................................................... 0.1g

Preparation of D-Alanine Solution: Add D-alanine to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Streptomycin Sulfate Solution: Composition per 10.0mL Streptomycin sulfate ..................................................................... 2.5g

Preparation of Streptomycin Sulfate Solution: Add streptomycin sulfate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add agar, tryptose, NaCl, and beef extract to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 4.0mL of the sterile glucose solution, 2.0mL of the sterile thymine solution, 2.0mL of the sterile alanine solution, and 1.6mL of the sterile streptomycin sulfate solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Bacillus subtilis. © 2010 by Taylor and Francis Group, LLC

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2–7.4. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Leuconostoc species. TC Amino Acids, HeLa 100X See: Tissue Culture Amino Acids, HeLa 100X TC Amino Acids, Minimal Eagle 50X See: Tissue Culture Amino Acids, Minimal Eagle 50X TC Dulbecco Solution See: Tissue Culture Dulbecco Solution TC Earle Solution See: Tissue Culture Earle Solution TC Hanks Solution See: Tissue Culture Hanks Solution TC Medium 199 See: Tissue Culture Medium 199 TC Medium Eagle, HeLa See: Tissue Culture Medium Eagle, HeLa

TCG Medium

TC Medium Eagle with Earle BSS See: Tissue Culture Medium Eagle with Earle Balanced Salt Solution TC Medium Eagle with Hanks BSS See: Tissue Culture Medium Eagle with Hank’s Balanced Salt Solution TC Medium Ham F10 See: Tissue Culture Medium Ham F10

1687

Plant peptone No. 3..................................................................... 15.0g NaCl............................................................................................ 10.0g Sodium citrate............................................................................. 10.0g Na2S2O3 ...................................................................................... 10.0g Yeast extract.................................................................................. 6.0g Synthetic detergent No. II............................................................. 2.0g Ferric citrate.................................................................................. 1.0g Thymol Blue ............................................................................... 0.04g Bromthymol Blue ....................................................................... 0.04g pH 8.6 ± 0.2 at 25°C

TC Medium NCTC 109 See: Tissue Culture Medium NCTC 109

Source: This medium is available as a premixed powder from Hi-

TC Medium RPMI #1640 See: Tissue Culture Medium RPMI #1640

water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Do not autoclave. Cool to 45°–50°C. Pour into sterile Petri dishes or distribute into sterile tubes.

TC Minimal Medium Eagle Spinner Modified MEM-S See: Tissue Culture Minimal Medium Eagle Spinner Modified TC Minimal Medium Eagle with Earle BSS See: Tissue Culture Minimal Medium Eagle with Earle Balanced Salts Solution TC Tyrode Solution See: Tissue Culture Tyrode Solution TC Vitamins Minimal Eagle, 100X See: Tissue Culture Vitamins Minimal Eagle, 100X

TCBS Agar (Thiosulfate Citrate Bile Salt Sucrose Agar) Composition per liter: Sucrose........................................................................................ 20.0g Agar ............................................................................................ 14.0g NaCl ............................................................................................ 10.0g Sodium citrate ............................................................................. 10.0g Na2S2O3 ...................................................................................... 10.0g Yeast extract.................................................................................. 5.0g Pancreatic digest of casein ............................................................ 5.0g Peptic digest of animal tissue........................................................ 5.0g Oxgall............................................................................................ 5.0g Sodium cholate ............................................................................. 3.0g Ferric citrate .................................................................................. 1.0g Thymol Blue ............................................................................... 0.04g Bromthymol Blue ....................................................................... 0.04g pH 8.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Do not autoclave. Cool to 45°–50°C. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation of Vibrio cholerae and Vibrio parahaemolyticus from a variety of clinical and nonclinical specimens.

TCBS HiVeg Agar

Media.

Preparation of Medium: Add components to distilled/deionized

Use: For the selective isolation of Vibrio cholerae and Vibrio parahaemolyticus from a variety of clinical and nonclinical specimens. For the cultivation of enteropathogenic vibrios causing food poisoning.

TCBS HiVeg Agar (Selective) Composition per liter: Sucrose........................................................................................ 20.0g Agar ............................................................................................ 15.0g Plant special peptone .................................................................. 14.5g NaCl............................................................................................ 10.0g Sodium citrate............................................................................. 10.0g Na2S2O3 ...................................................................................... 10.0g Yeast extract.................................................................................. 5.0g Synthetic detergent No. II............................................................. 2.0g Synthetic detergent No. IV ........................................................... 1.5g Ferric citrate.................................................................................. 1.0g BromthymolBlue ........................................................................ 0.04g Thymol Blue ............................................................................... 0.04g pH 8.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Use: For the selective isolation of Vibrio cholerae and other enteropathogenic vibrios.

TCG Medium (DSMZ Medium 1009) Composition per liter: Casitone ....................................................................................... 5.0g Glucose ........................................................................................ 4.0g Tryptone ....................................................................................... 3.0g Artificial seawater.........................................................................1.0L pH 7.5 ± 0.2 at 25°C

Artificial Seawater: Composition per 10.0mL:

Composition per liter:

Sea salts, Instant Ocean .............................................................. 22.0g

Preparation of Artificial Seawater: Add Instant Ocean sea salts

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

1688

TCH Medium

Preparation of Medium: Add components to artificial seawater and bring volume to 1.0L. Mix thoroughly. Distribute to tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation of Mechercharimyces mesophilus.

TCH Medium (Thiophene 2 Carboxylic Acid Hydrazide Medium) Composition per 1105.0mL: Thiophene-2-carboxylic acid hydrazide .................................... 1.1mg Middlebrook 7H10 agar base........................................................1.0L OADC enrichment .................................................................100.0mL Glycerol .....................................................................................5.0mL pH 6.6 ± 0.2 at 25°C

Middlebrook 7H10 Agar Base: Composition per liter: Agar ............................................................................................ 15.0g Na2HPO4 ....................................................................................... 1.5g KH2PO4 ......................................................................................... 1.5g (NH4)2SO4 ..................................................................................... 0.5g L-Glutamic acid ............................................................................. 0.5g Sodium citrate ............................................................................... 0.4g Ferric ammonium citrate............................................................. 0.04g MgSO4·7H2O ............................................................................ 0.025g ZnSO4·7H2O .............................................................................. 1.0mg CuSO4·5H2O .............................................................................. 1.0mg Pyridoxine .................................................................................. 1.0mg Biotin ......................................................................................... 0.5mg CaCl2·2H2O................................................................................ 0.5mg Malachite Green...................................................................... .0.25mg

Preparation of Middlebrook 7H10 Agar Base: Add glycerol to 900.0mL of distilled/deionized water and add remaining components. Mix thoroughly. Gently heat and bring to boiling.

Middlebrook OADC Enrichment: Composition per 100.0mL: Bovine albumin fraction V ........................................................... 5.0g Glucose ......................................................................................... 2.0g NaCl ............................................................................................ 0.85g Oleic acid .................................................................................... 0.05g Catalase ...................................................................................... 4.0mg

Source: This enrichment is available as a prepared enrichment from BD Diagnostic Systems.

Preparation of Middlebrook OADC Enrichment: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation for Medium: Combine components. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the differentiation of Mycobacterium species based on sensitivity to TCH. Mycobacterium bovis is inhibited by TCH. Mycobacterium tuberculosis and other mycobacteria are generally resistant to low concentrations of TCH. This distinguishes Mycobacterium bovis from other nonchromogenic, slow-growing mycobacteria.

TCY Agar Composition per liter: NaCl ............................................................................................ 31.3g Agar ............................................................................................ 15.0g MgCl2·6H2O ............................................................................... 10.8g © 2010 by Taylor and Francis Group, LLC

CaCl2·2H2O .................................................................................. 1.0g Casamino acids ............................................................................. 1.0g Tryptone........................................................................................ 1.0g KCl................................................................................................ 0.7g Yeast extract.................................................................................. 0.2g pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Flexibacter maritimus.

TCY Broth Composition per liter: NaCl............................................................................................ 31.3g Agar ............................................................................................ 15.0g MgCl2·6H2O ............................................................................... 10.8g CaCl2·2H2O .................................................................................. 1.0g Casamino acids ............................................................................. 1.0g Tryptone........................................................................................ 1.0g KCl................................................................................................ 0.7g Yeast extract.................................................................................. 0.2g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Flexibacter maritimus.

TD3 Medium (DSMZ Medium 876) Composition per 1078.0mL: NaCl............................................................................................ 20.0g MgCl2·6H2O ................................................................................. 9.8g Na2SO4 .......................................................................................... 4.0g KCl................................................................................................ 0.5g NH4Cl ......................................................................................... 0.25g KH2PO4......................................................................................... 0.2g CaCl2·2H2O .................................................................................. 0.1g Resazurin ................................................................................... 0.5mg NaHCO3 solution .....................................................................50.0mL Na2S·9H2O solution .................................................................13.0mL Sodium caproate solution ........................................................10.0mL Selenite-tungstate solution.........................................................2.0mL Seven vitamin solution ..............................................................1.0mL Vitamin solution.........................................................................1.0mL Trace elements solution SL-10 ..................................................1.0mL pH 6.8 ± 0.2 at 25°C Na2S·9H2O Solution: Composition per 20.0mL: Na2S·9H2O.................................................................................... 0.6g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature. NaHCO3 Solution: Composition per 10.0mL: NaHCO3 ........................................................................................ 5.0g

TDN Broth

1689

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Filter sterilize.

Preparation of Selenite-Tungstate Solution: Add components

Sodium Caproate Solution: Composition per 10.0mL:

Optional Supplemental Fatty Acid Mixture: Composition per 20.0mL:

Sodium caproate ........................................................................... 0.5g

Valeric acid ................................................................................... 0.5g Isovaleric acid............................................................................... 0.5g Alpha-Methylbutyric acid............................................................. 0.5g Isobutyric acid .............................................................................. 0.5g

Preparation of Sodium Caproate Solution: Add sodium caproate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Seven Vitamin Solution: Composition per liter: Pyridoxine hydrochloride ...................................................... 300.0mg Thiamine-HCl·2H2O .............................................................. 200.0mg Nicotinic acid ......................................................................... 200.0mg Vitamin B12 ............................................................................ 100.0mg Calcium pantothenate ............................................................ 100.0mg p-Aminobenzoic acid............................................................... 80.0mg D(+)-Biotin ............................................................................... 20.0mg

Preparation of Seven Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Sparge with 100% N2. Mix thoroughly. Filter sterilize. Vitamin Solution: Composition per 100.0mL: Pyridoxine-HCl ........................................................................ 10.0mg Thiamine-HCl·2H2O .................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg D-Ca-pantothenate...................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 100.0mL. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize. Trace Elements Solution SL-10: Composition per liter: FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O........................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution) ..................................................................10.0mL

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Preparation of Optional Supplemental Fatty Acid Mixture: Add components to 20.0mL distilled/deionized water. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Preparation of Medium: Prepare and dispense medium under 80% N2 + 20% CO2 gas atmosphere. Add components, except NaHCO3 solution, sodium caproate solution, Na2S·9H2O solution, vitamin solution, seven vitamin solution, selenite-tungstate solution, and trace elements solution SL-10, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add 50.0mL NaHCO3 solution, 10.0mL sodium caproate solution, 10.0mL Na2S·9H2O solution, 1.0mL vitamin solution, 1.0mL seven vitamin solution, 2.0mL selenite-tungstate solution, and 1.0mL trace elements solution SL-10. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or bottles. Growth can be stimulated by addition of clarified rumen fluid or 10–20mL of a mixture of fatty acids. Final pH should be 6.7–6.9.

Use: For the cultivation of unclassified bacterium DSM 13418.

TDC Medium Composition per liter: Agar ............................................................................................ 20.0g CaCO3 ......................................................................................... 10.0g Glucose ......................................................................................... 5.0g K2HPO4......................................................................................... 1.0g MgSO4 .......................................................................................... 1.0g

TDN Broth (DSMZ Medium 1012) Composition per liter:

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C.

Pancreatic digest of gelatin........................................................... 0.5g Casamino acids ............................................................................ 0.5g Beef extract................................................................................... 0.3g Yeast extract ................................................................................. 0.1g Magnesium chloride solution ..................................................10.0mL Calcium chloride solution........................................................10.0mL pH 7.2 ± 0.2 at 25°C

Selenite-Tungstate Solution Composition per liter:

Calcium Chloride Solution: Composition per 10.0mL:

NaOH ............................................................................................ 0.5g Na2WO4·2H2O ........................................................................... 4.0mg Na2SeO3·5H2O........................................................................... 3.0mg

Preparation of Calcium Chloride Solution: Add CaCl2·2H2O

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

CaCl2·2H2O .................................................................................. 0.3g to distilled/deionized water and bring volume to 10.0mL. Mix thor-

1690

Tea Fungus Medium

oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Magnesium Chloride Solution: Composition per 10.0mL: MgCl2·6H2O.................................................................................. 0.6g

Preparation of Magnesium Chloride Solution: Add MgCl2·6H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Preparation of Medium: Add components, except calcium chloride and magnesium chloride solutions, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Adjust the pH to 7.2 with NaOH. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aspetically add calcium chloride and magnesium chloride solutions. Mix thoroughly.

Use: For the cultivation of Bdellovibrio bacteriovorus.

Tea Fungus Medium (DSMZ Medium 268) Composition per liter: Sucrose........................................................................................ 50.0g Tea leaves, black ........................................................................... 5.0g Add tea and sucrose to a flask. Add 1.0L freshly boiled tap water. Allow to stand for 15 min. Filter and cool to room temperature. Place a small disc of a cork stopper that has been steamed for 15 min on 2 successive days on the surface of the tea. Place the inoculum onto the cork. Cover the beaker with aluminum foil and incubate.

Use: For the cultivation of tea fungus.

TEC Agar (m-TEC Agar)

Tech Agar Composition per liter: Pancreatic digest of gelatin......................................................... 20.0g Agar ............................................................................................ 13.6g K2SO4·7H2O ............................................................................... 10.0g MgCl2·6H2O ................................................................................. 1.4g Glycerol ...................................................................................10.0mL pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components, except glycerol, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Add glycerol. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the production of pyocyanin pigment by Pseudomonas species.

Teepol Broth, Enriched (m-Teepol Broth, Enriched) Composition per liter: Peptone ....................................................................................... 40.0g Lactose........................................................................................ 30.0g Yeast extract.................................................................................. 6.0g Phenol Red.................................................................................... 0.2g Sodium lauryl sulfate (Teepol, 0.1% solution) .......................................................4.0mL pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the enumeration of coliform organisms and Escherichia coli in water by the membrane filter method.

Composition per liter: Agar ............................................................................................ 15.0g Lactose ........................................................................................ 10.0g NaCl .............................................................................................. 7.5g Proteose peptone ........................................................................... 5.0g K2HPO4 ......................................................................................... 3.3g Yeast extract.................................................................................. 3.0g KH2PO4 ......................................................................................... 1.0g Sodium lauryl sulfate .................................................................... 0.2g Sodium deoxycholate.................................................................... 0.1g Bromcresol Purple ...................................................................... 0.08g Bromphenol Red ......................................................................... 0.08g pH 5.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 5.0. Sterilization is unnecessary. Pour into sterile Petri dishes or distribute into sterile tubes or flasks. Store at 2°–8°C. Use within 1 week.

Use: For detection of Escherichia coli in recreational waters by the membrane filter method. This agar is used in conjunction with a urea substrate to detect urease production. After addition of the urea substrate, Escherichia coli appears as yellow-yellow/brown colonies when viewed under a fluorescent lamp. © 2010 by Taylor and Francis Group, LLC

Teepol HiVeg Broth Composition per liter: Plant peptone .............................................................................. 20.0g Lactose........................................................................................ 10.0g NaCl.............................................................................................. 5.0g Teepol ........................................................................................... 1.0g Phenol Red.................................................................................. 0.02g pH 7.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the enumeration of coliform organisms and Escherichia coli in water by the membrane filter method. For the selective isolation and identification of enteric, lactose-fermenting bacteria. Tellurite Blood Agar See: Chocolate Tellurite Agar

Tellurite Glycine Agar Composition per liter: Agar ............................................................................................ 17.5g Pancreatic digest of casein.......................................................... 10.0g Glycine........................................................................................ 10.0g

Tepidanaerobacter Medium

1691

Yeast extract.................................................................................. 6.5g D-Mannitol..................................................................................... 5.0g K2HPO4 ......................................................................................... 5.0g LiCl ............................................................................................... 5.0g Enzymatic hydrolysate of soybean meal ...................................... 3.5g Chapman tellurite solution.......................................................10.0mL pH 7.2 ± 0.2 at 25°C

Use: For the quantitative detection of coagulase-positive staphylococci from foods and other sources.

Source: This medium is available as a premixed powder from BD Di-

Agar ............................................................................................ 20.0g Na2HPO4·12H2O........................................................................... 9.0g Uric acid........................................................................................ 4.0g Pancreatic digest of casein............................................................ 1.7g KH2PO4......................................................................................... 1.5g NaCl.............................................................................................. 0.5g Papaic digest of soybean meal...................................................... 0.3g K2HPO4....................................................................................... 0.25g Glucose ....................................................................................... 0.25g MgSO4·7H2O ................................................................................ 0.2g CaCl2 ........................................................................................... 0.02g Ferric ammonium citrate............................................................ 1.2mg MnCl2·4H2O .............................................................................. 1.0mg pH 7.2 ± 0.2 at 25°C

agnostic Systems.

Caution: Lithium chloride is harmful. Avoid bodily contact and inhalation of vapors. On contact with skin wash with plenty of water immediately.

Chapman Tellurite Solution: Composition per 100.0mL: K2TeO3 .......................................................................................... 1.0g

Preparation of Chapman Tellurite Solution: Add K2TeO3 to

distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Caution: Potassium tellurite is toxic. Preparation of Medium: Add components, except Chapman tellurite solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 10.0mL of sterile Chapman tellurite solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Allow the surface of the plates to dry before inoculating.

Use: For the isolation and cultivation of coagulase-positive staphylococci.

Tellurite Glycine Agar Composition per liter: Agar ............................................................................................ 16.0g Pancreatic digest of casein .......................................................... 10.0g Glycine........................................................................................ 10.0g Yeast extract.................................................................................. 5.0g D-Mannitol..................................................................................... 5.0g K2HPO4 ......................................................................................... 5.0g LiCl ............................................................................................... 5.0g Chapman tellurite solution.......................................................20.0mL pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems. Chapman Tellurite Solution: Composition per 100.0mL: K2TeO3 .......................................................................................... 1.0g

Preparation of Chapman Tellurite Solution: Add K2TeO3 to

distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Caution: Potassium tellurite is toxic. Preparation of Medium: Add components, except Chapman tellurite solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 20.0mL of sterile Chapman tellurite solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Allow the surface of the plates to dry before inoculating. © 2010 by Taylor and Francis Group, LLC

Tellurite Polymyxin Egg Yolk Agar See: TPEY Agar

TEP Uric Acid Medium Composition per liter:

Use: For the cultivation and maintenance of Bacillus fastidiosus and other microorganisms that can utilize uric acid as a carbon source.

Tepidanaerobacter Medium (DSMZ Medium 1051) Composition per liter: Yeast extract ................................................................................. 2.3g NH4Cl ........................................................................................ 0.54g MgCl2·6H2O ................................................................................. 0.2g CaCl2·2H2O ................................................................................ 0.15g KH2PO4....................................................................................... 0.14g Resazurin .................................................................................. 0.5mg Bicarbonate solution ................................................................10.0mL Glucose solution ......................................................................10.0mL Sulfide solution........................................................................10.0mL Cysteine solution .....................................................................10.0mL Trace elements solution .............................................................1.0mL Vitamin solution.........................................................................2.0mL pH 7.1 ± 0.2 at 25°C

Trace Elements Solution: Composition per liter: Nitrilotriacetic acid ..................................................................... 12.8g FeCl3·6H2O................................................................................. 1.35g NaCl.............................................................................................. 1.0g NiCl2·6H2O................................................................................. 0.12g MnCl2·4H2O ................................................................................. 0.1g CaCl2·2H2O .................................................................................. 0.1g ZnCl2 ............................................................................................. 0.1g Na2SeO3·5H2O.......................................................................... 0.026g CuCl2·2H2O .............................................................................. 0.025g CoCl2·6H2O .............................................................................. 0.024g Na2MoO4·2H2O ........................................................................ 0.024g H3BO3 ......................................................................................... 0.01g

1692

Tepidibacter Medium

Preparation of Trace Elements Solution: Add nitrilotriacetic acid to approximately 500.0mL of distilled/deionized water. Dissolve by adding KOH and adjust pH to 6.5. Add remaining components. Bring volume to 1.0L with additional distilled/deionized water. Adjust pH to 7.0 with KOH. Glucose Solution: Composition per 10.0mL: D-Glucose...................................................................................... 2.2g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Sulfide Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.3g

Preparation of Sulfide Solution: Add Na2S·9H2O to distilled/de-

ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Bicarbonate Solution: Composition per 10.0mL: NaHCO3 ........................................................................................ 2.5g

Tepidibacter Medium (DSMZ Medium 985) Composition per liter: NaCl ........................................................................................... 18.0g Casein ........................................................................................ 10.0g MgCl2·6H2O ................................................................................. 4.0g KCl ............................................................................................. 0.34g NH4Cl ........................................................................................ 0.25g Yeast extract ................................................................................. 0.2g KH2PO4....................................................................................... 0.18g CaCl2·2H2O ................................................................................ 0.11g Fe(NH4)2(SO4)2·6H2O ............................................................... 0.02g Resazurin .................................................................................. 1.0mg Bicarbonate solution ................................................................10.0mL Vitamin solution.......................................................................10.0mL Sulfide solution........................................................................10.0mL Trace elements solution SL-10 ..................................................1.0mL pH 6.8 ± 0.2 at 25°C

Sulfide Solution: Composition per 10.0mL: Na2S·9H2O.................................................................................... 0.5g

Preparation of Sulfide Solution: Add Na2S·9H2O to distilled/de-

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/

ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Cysteine Solution: Composition per 10.0mL:

Bicarbonate Solution: Composition per 10.0mL:

deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with a gas mixture of 80% N2 + 20% CO2. Filter sterilize.

L-Cysteine-HCl·2H2O ................................................................... 0.3g

Preparation of Cysteine Solution: Add L-cysteine to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Vitamin Solution: Composition per liter: Pyridoxine-HCl ........................................................................ 10.0mg Thiamine-HCl·2H2O .................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg D-Ca-pantothenate...................................................................... 5.0mg p-Aminobenzoic acid ................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Preparation of Medium: Add components, except vitamin, bicarbonate, glucose, cysteine, and sulfide solutions, to distilled/deionized water and bring volume to 958.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for 1 min. Cool to room temperature while sparging with a gas mixture of 80% N2 + 20% CO2. Dispense into culture vessels under an atmosphere of 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aspetically add vitamin, bicarbonate, glucose, cysteine, and sulfide solutions. Mix thoroughly. Adjust the pH to 7.0–7.2.

NaHCO3 ........................................................................................ 5.0g

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with gas mixture of 80% N2 + 20% CO2. Filter sterilize.

Vitamin Solution: Composition per liter: Pyridoxine-HCl........................................................................ 10.0mg Thiamine-HCl·2H2O.................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg D-Ca-pantothenate ..................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize. Trace Elements Solution SL-10: Composition per liter: FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Teredinobacter Medium Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure– 121°C.

Preparation of Medium: Add components, except vitamin, bicarbonate, and sulfide solutions, to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for 1 min. Cool to room temperature while sparging with a gas mixture of 80% N2 + 20% CO2. Dispense into culture vessels under an atmosphere of 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aspetically add vitamin, bicarbonate, and sulfide solutions. Mix thoroughly. Adjust the pH to 6.6–7.0.

Use: For the cultivation of Tepidibacter thalassicus and Deferribacter autotrophicus.

Tepidibacter Medium with Peptone (DSMZ Medium 985) Composition per liter: NaCl ........................................................................................... 18.0g Peptone........................................................................................ 10.0g MgCl2·6H2O.................................................................................. 4.0g KCl ............................................................................................. 0.34g NH4Cl ........................................................................................ 0.25g Yeast extract ................................................................................. 0.2g KH2PO4 ....................................................................................... 0.18g CaCl2·2H2O................................................................................. 0.11g Fe(NH4)2(SO4)2·6H2O ............................................................... 0.02g Resazurin .................................................................................. 1.0mg Bicarbonate solution ................................................................10.0mL Vitamin solution.......................................................................10.0mL Sulfide solution ........................................................................10.0mL Trace elements solution SL-10 ..................................................1.0mL Wolfe's mineral elixir.................................................................1.0mL pH 6.8 ± 0.2 at 25°C

Sulfide Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.5g

Preparation of Sulfide Solution: Add Na2S·9H2O to distilled/de-

ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Bicarbonate Solution: Composition per 10.0mL: NaHCO3 ........................................................................................ 5.0g

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with a gas mixture of 80% N2 + 20% CO2. Filter sterilize.

1693

Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize. Trace Elements Solution SL-10: Composition per liter: FeCl2·4H2O................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O.............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

Wolfe’s Mineral Elixir: Composition per liter: MgSO4·7H2O .............................................................................. 30.0g NaCl............................................................................................ 10.0g MnSO4·2H2O ................................................................................ 5.0g (NH4)2NiSO4·6H2O ...................................................................... 2.8g CoCl2·6H2O .................................................................................. 1.8g ZnSO4·7H2O ................................................................................. 1.8g FeSO4·7H2O.................................................................................. 1.0g CaCl2·2H2O .................................................................................. 1.0g KAl(SO4)2·12H2O....................................................................... 0.18g CuSO4·5H2O................................................................................. 0.1g H3BO3 ........................................................................................... 0.1g Na2MoO4·2H2O ............................................................................ 0.1g Na2SeO4 ........................................................................................ 0.1g Na2WO4·2H2O .............................................................................. 0.1g

Preparation of Wolfe’s Mineral Elixir: Adjust pH of 1.0L of distilled/deionized water to 1.0 with dilute H2SO4. Add remaining components one at a time. Mix throughly to dissolve. Preparation of Medium: Add components, except vitamin, bicarbonate, and sulfide solutions, to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for 1 min. Cool to room temperature while sparging with a gas mixture of 80% N2 + 20% CO2. Dispense into culture vessels under an atmosphere of 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aspetically add vitamin, bicarbonate, and sulfide solutions. Mix thoroughly. Adjust the pH to 6.6–7.0.

Use: For the cultivation of Clostridium tepidiprofundi.

Teredinobacter Medium Composition 1010.0mL: Solution A................................................................................50.0mL Solution C ..............................................................................750.0mL Solution B ..............................................................................200.0mL Solution D................................................................................10.0mL

1694

Tergitol 7 Agar

Solution A: Composition per 50.0mL:

TTC Solution: Composition per 100.0mL:

K2HPO4 .................................................................................... 20.0mg Na2CO3 ................................................................................... 20.0mg Ferric ammonium citrate............................................................ 6.0mg EDTA ......................................................................................... 1.0mg Trace metal mix A5....................................................................1.0mL

Triphenyltetrazolium chloride .................................................... 0.05g

Trace Metal Mix A5: Composition per liter: H3BO3 ......................................................................................... 2.86g MnCl2·4H2O................................................................................ 1.81g ZnSO4·7H2O ............................................................................. 0.222g Na2MoO4·2H2O .......................................................................... 0.39g CuSO4·5H2O ............................................................................. 0.079g Co(NO3)2·6H2O ....................................................................... 49.4mg

Preparation of Trace Metal Mix A5: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Solution A: Add components to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Solution B: Composition per 200.0mL: Agar, noble.................................................................................... 2.0g

Preparation of Solution B: Add agar to distilled/deionized water and bring volume to 200.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Solution C: Composition per 750.0mL: Seawater.................................................................................750.0mL

Preparation of Solution C: Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Solution D: Composition per 10.0mL: Cellulose, Sigmacell Type 101 ..................................................... 1.0g

Preparation of Solution D: Add cellulose to distilled/deionized

Preparation of TTC Solution: Add triphenyltetrazolium chloride to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 995.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 5.0mL of sterile TTC solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the detection and enumeration of coliforms. Lactose-fermenting bacteria appear as yellow colonies. Lactose-nonfermenting bacteria appear as blue colonies.

Tergitol 7 Agar Composition per liter: Agar ............................................................................................ 15.0g Lactose........................................................................................ 10.0g Yeast extract.................................................................................. 3.0g Pancreatic digest of casein............................................................ 2.5g Peptic digest of animal tissue ....................................................... 2.5g Tergitol 7....................................................................................... 0.1g Bromthymol Blue .................................................................... 25.0mg TTC solution..............................................................................3.0mL pH 6.9 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

TTC Solution: Composition per 100.0mL: Triphenyltetrazolium chloride ...................................................... 1.0g

Preparation of TTC Solution: Add triphenyltetrazolium chloride to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components to distilled/deionized

Preparation of Medium: Aseptically combine 50.0mL of sterile

water and bring volume to 997.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 3.0mL of sterile TTC solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

solution A, 200.0mL of sterile solution B, 750.0mL of sterile solution C, and 10.0mL of sterile solution D. Mix thoroughly. Aseptically distribute into sterile tubes.

Use: For the selective isolation and differentiation of coliform bacteria based on lactose fermentation. Lactose-fermenting bacteria appear as yellow colonies. Lactose-nonfermenting bacteria appear as blue colonies.

water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Use: For the cultivation of unidentified bacterium ATCC 39867.

Tergitol 7 Agar H Tergitol 7 Agar

Composition per liter:

Lactose ........................................................................................ 20.0g Agar ............................................................................................ 13.0g Peptone........................................................................................ 10.0g Yeast extract.................................................................................. 6.0g Meat extract .................................................................................. 5.0g Tergitol-7....................................................................................... 0.1g Bromthymol Blue ....................................................................... 0.05g TTC solution ..............................................................................5.0mL pH 7.2 ± 0.2 at 25°C

Agar ............................................................................................ 15.0g Lactose........................................................................................ 10.0g Yeast extract.................................................................................. 3.0g Pancreatic digest of casein............................................................ 2.5g Peptic digest of animal tissue ....................................................... 2.5g Ferric ammonium citrate............................................................... 0.5g Na2S2O3 ........................................................................................ 0.5g Tergitol 7....................................................................................... 0.1g Bromthymol Blue ..................................................................... 0.025g pH 7.2 ± 0.2

Source: This medium is available as a premixed powder from Oxoid

Preparation of Medium: Add components to distilled/deionized

Unipath.

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

Composition per liter:

Termitobacter Medium

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

identification of coliform organisms, as per Indian Standard published by BIS.

Use: For the selective isolation and differentiation of enteric bacteria from urine.

Tergitol 7 Broth Composition per liter: Lactose ........................................................................................ 10.0g Yeast extract.................................................................................. 3.0g Pancreatic digest of casein ............................................................ 2.5g Peptic digest of animal tissue........................................................ 2.5g Tergitol 7 ....................................................................................... 0.1g Bromthymol Blue .................................................................... 25.0mg TTC solution ..............................................................................3.0mL pH 6.9 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

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Tergitol 7 HiVeg Agar H Composition per liter: Agar ............................................................................................ 15.0g Lactose........................................................................................ 10.0g Plant peptone No. 3....................................................................... 5.0g Yeast extract.................................................................................. 3.0g Ferric ammonium citrate............................................................... 0.5g Na2S2O3 ........................................................................................ 0.5g Tergitol 7....................................................................................... 0.1g Bromthymol Blue ..................................................................... 0.025g pH 7.2 ± 0.2

TTC Solution: Composition per 100.0mL:

Use: For the selective isolation and differentiation of enteric bacteria

Triphenyltetrazolium chloride....................................................... 1.0g

from urine.

Preparation of TTC Solution: Add triphenyltetrazolium chloride to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 997.0mL. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 3.0mL of sterile TTC solution. Mix thoroughly.

Use: For the isolation and cultivation of coliforms, Salmonella, and other enteric bacteria.

Tergitol 7 HiVeg Agar Base with TTC Composition per liter: Agar ............................................................................................ 15.0g Lactose ........................................................................................ 10.0g Plant peptone No. 3....................................................................... 5.0g Yeast extract.................................................................................. 3.0g Sodium heptadecyl sulfate ............................................................ 0.1g Bromthymol Blue ..................................................................... 0.025g TTC solution ..............................................................................5.0mL pH 7.2 ± 0.2 at 25°C

Source: This medium, without TTC solution, is available as a premixed powder from HiMedia.

TTC Solution: Composition per 100.0mL: Triphenyltetrazolium chloride..................................................... 0.05g

Preparation of TTC Solution: Add triphenyltetrazolium chloride to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Use: For the detection and enumeration of coliforms. Lactose-fermenting bacteria appear as yellow colonies. Lactose-nonfermenting bacteria appear as blue colonies. For the selective enumeration and © 2010 by Taylor and Francis Group, LLC

Tergitol-7 HiVeg Broth Composition per liter: Lactose........................................................................................ 10.0g Plant peptone No. 3....................................................................... 5.0g Yeast extract.................................................................................. 3.0g Tergitol 7....................................................................................... 0.1g Bromthymol Blue ..................................................................... 0.025g pH 6.9 ± 0.2

Source: This medium is available as a premixed powder from HiMedia.

Use: For the selective and differential medium for detection and enumeration of coliforms.

Termitobacter Medium Composition per 1060.0mL NH4Cl ........................................................................................... 1.0g NaCl.............................................................................................. 0.6g Sodium acetate·3H2O.................................................................... 0.5g L-Cysteine·HCl ............................................................................. 0.5g K2HPO4......................................................................................... 0.3g KH2PO4......................................................................................... 0.3g MgCl2·6H2O ................................................................................. 0.2g CaCl2·2H2O .................................................................................. 0.1g KCl................................................................................................ 0.1g Yeast extract.................................................................................. 0.2g Resazurin ................................................................................... 0.5mg NaHCO3 solution .....................................................................40.0mL Na2S·9H2O solution .................................................................10.0mL Trans-3,4,5-trimethoxycinnamate solution..............................10.0mL Trace elements solution SL-10 ..................................................1.5mL

NaHCO3 Solution: Composition per 10.0mL: NaHCO3 ........................................................................................ 1.0g

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Terrific Broth

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure– 121°C. Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.3g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Before use, neutralize to pH 7.0 with sterile HCl.

Preparation of Buffer Solution: Add components to 100.0mL distilled/deionized water. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Ampicillin Solution: Composition per 10.0mL: Ampicillin .................................................................................... 50μg

Preparation of Ampicillin Solution: Add ampicillin to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Trans-3,4,5-trimethoxycinnamate............................................... 1.19g

Preparation of Trans-3,4,5-Trimethoxycinnamate Solution:

Use: For the cultivation of plasmid-bearing strains of Escherichia coli.

Add trans-3,4,5-trimethoxycinnamate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Neutralize with NaOH. Autoclave for 15 min at 15 psi pressure–121°C.

Terrific Broth with 100 μg/ml Ampicillin

Trans-3,4,5-Trimethoxycinnamate Solution: Composition per 10.0mL:

Preparation of Medium: Prepare and dispense medium under 80%

(ATCC Medium 1946) Composition per liter:

N2 + 20% CO2 gas mixture. Add components, except NaHCO3 solution, trans-3,4,5-trimethoxycinnamate solution, and Na2S·9H2O solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% N2 + 20% CO2 gas mixture. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add 40.0mL of sterile NaHCO3 solution, 10.0mL of sterile trans-3,4,5trimethoxycinnamate solution, and 10.0mL of sterile Na2S·9H2O solution. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or bottles.

Yeast extract................................................................................ 24.0g Tryptone...................................................................................... 12.0g Glycerol ...................................................................................... 12.0g Buffer solution .......................................................................100.0mL Ampicillin solution ..................................................................10.0mL pH 7.2 ± 0.2 at 25°C

Use: For the cultivation of Sporobacter termitidis.

KH2PO4....................................................................................... 2.31g K2HPO4..................................................................................... 12.54g

Terrific Broth Composition per liter: Yeast extract................................................................................ 24.0g Tryptone ...................................................................................... 12.0g KH2PO4 ......................................................................................... 9.4g K2HPO4 ......................................................................................... 2.2g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of plasmid-bearing strains of Escherichia coli.

Terrific Broth with 50 μg/ml Ampicillin (ATCC Medium 2140) Composition per liter: Yeast extract................................................................................ 24.0g Tryptone ...................................................................................... 12.0g Glycerol ...................................................................................... 12.0g Buffer solution .......................................................................100.0mL Ampicillin solution ..................................................................10.0mL pH 7.2 ± 0.2 at 25°C

Buffer Solution: Composition per 100.0mL: KH2PO4 ....................................................................................... 2.31g K2HPO4 ..................................................................................... 12.54g © 2010 by Taylor and Francis Group, LLC

Buffer Solution: Composition per 100.0mL:

Preparation of Buffer Solution: Add components to 100.0mL distilled/deionized water. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Ampicillin Solution: Composition per 10.0mL: Ampicillin .................................................................................. 100μg

Preparation of Ampicillin Solution: Add ampicillin to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except buffer and ampicillin solutions, to 890.0mL distilled/deionized water. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add the buffer and ampicillin solutions. Mix thoroughly. Aseptically distribute to tubes or flasks. Use: For the cultivation of plasmid-bearing strains of Escherichia coli. For the cloning of p3PK plasmid for studying intracellular protein targeting, pSH plasmid which is a strong mammalian expression vector, and pOT182 plasmid for the construction and use of a self-cloning promoter probe vector for Gram-negative bacteria.

Terrific Broth with 200 μg/ml Ampicillin (ATCC Medium 1945) Composition per liter: Yeast extract................................................................................ 24.0g Tryptone...................................................................................... 12.0g Glycerol ...................................................................................... 12.0g

Tetracycline Luria Agar No. 2

Buffer solution .......................................................................100.0mL Ampicillin solution ..................................................................10.0mL pH 7.2 ± 0.2 at 25°C

Buffer Solution: Composition per 100.0mL:

1697

Yeast extract.................................................................................. 5.0g NaCl.............................................................................................. 0.5g Glucose solution ......................................................................20.0mL Tetracycline solution................................................................10.0mL pH 7.0 ± 0.2 at 25°C

KH2PO4 ....................................................................................... 2.31g K2HPO4 ..................................................................................... 12.54g

Glucose Solution: Composition per 50.0mL:

Preparation of Buffer Solution: Add components to 100.0mL

Glucose ......................................................................................... 5.0g

distilled/deionized water. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.

Ampicillin Solution: Composition per 10.0mL: Ampicillin ...............................................................................200.0μg

Tetracycline Solution: Composition per 10.0mL:

Preparation of Ampicillin Solution: Add ampicillin to distilled/

Tetracycline.............................................................................. 12.0mg

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Tetracycline Solution: Add tetracycline to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except buffer and ampicillin solutions, to 890.0mL distilled/deionized water. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add the buffer and ampicillin solutions. Mix thoroughly. Aseptically distribute to tubes or flasks. Use: For the cultivation of plasmid-bearing strains of Escherichia coli. For the cloning of pSVbeta, pCMVbeta, pTKbeta, pADbeta, pNASSbeta, and pBS-hTOP2 plasmids in E. coli.

Preparation of Medium: Add components, except tetracycline solution and glucose solution, to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Bring pH to 7.0. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 10.0mL of sterile tetracycline solution and 20.0mL of sterile glucose solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Escherichia coli.

Tetracycline L Broth Medium Composition per liter:

Tetracycline Luria Agar No. 2

Agar ............................................................................................ 15.0g Pancreatic digest of casein .......................................................... 10.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g Peptone.......................................................................................... 5.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 2.0g Beef extract ................................................................................... 1.0g Glucose ......................................................................................... 1.0g Tetracycline solution................................................................10.0mL pH 7.0 ± 0.2 at 25°C

Composition per liter:

Tetracycline Solution: Composition per 10.0mL:

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.

Tetracycline.............................................................................. 12.5mg

Glucose Solution: Composition per 50.0mL: Glucose ......................................................................................... 5.0g

Preparation of Tetracycline Solution: Add tetracycline to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Tetracycline Solution: Composition per 10.0mL:

Preparation of Medium: Add components, except tetracycline so-

Preparation of Tetracycline Solution: Add tetracycline to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

lution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Bring pH to 7.0. Autoclave for 15 min at 15 psi pressure– 121°C. Aseptically add 10.0mL of sterile tetracycline solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Escherichia coli.

Tetracycline Luria Agar No. 1 Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein .......................................................... 10.0g © 2010 by Taylor and Francis Group, LLC

Tetracycline.............................................................................. 10.0mg

Use: For the cultivation of Escherichia coli.

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Tetracycline Luria Agar No. 3

Tetracycline Luria Agar No. 3 Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein .......................................................... 10.0g Yeast extract.................................................................................. 5.0g NaCl .............................................................................................. 0.5g Glucose solution ......................................................................20.0mL Tetracycline solution................................................................10.0mL pH 7.0 ± 0.2 at 25°C

Glucose Solution: Composition per 50.0mL: Glucose ......................................................................................... 5.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize. Tetracycline Solution: Composition per 10.0mL: Tetracycline.............................................................................. 20.0mg

Preparation of Tetracycline Solution: Add tetracycline to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except tetracycline solution and glucose solution, to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Bring pH to 7.0. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 10.0mL of sterile tetracycline solution and 20.0mL of sterile glucose solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Escherichia coli.

Tetracycline TY Salt Medium Composition per liter: NaCl2........................................................................................... 10.0g Pancreatic digest of casein .......................................................... 10.0g Yeast extract.................................................................................. 5.0g Tetracycline solution................................................................10.0mL pH 7.0 ± 0.2 at 25°C

Tetracycline Solution: Composition per 10.0mL: Tetracycline.............................................................................. 12.5mg

Preparation of Tetracycline Solution: Add tetracycline to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except tetracycline solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Bring pH to 7.0. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0mL of sterile tetracycline solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Escherichia coli.

Tetrahymena Medium Composition per liter: Pancreatic digest of casein ............................................................ 5.0g Proteose peptone ........................................................................... 5.0g K2HPO4 ......................................................................................... 0.2g pH 7.2 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Distribute into screw-capped tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Tetrahymena americanis, Tetrahymena asiatica, Tetrahymena australis, Tetrahymena borealis, Tetrahymena canadensis, Tetrahymena capricornis, Tetrahymena caudata, Tetrahymena corlissi, Tetrahymena elliotti, Tetrahymena furgasoni, Tetrahymena hegewischi, Tetrahymena hyperangularis, Tetrahymena malaccensis, Tetrahymena mimbres, Tetrahymena nanney, Tetrahymena nipissingi, Tetrahymena pigmentosa, Tetrahymena pyriformis, Tetrahymena silvana, Tetrahymena sonneborni, Tetrahymena thermophila, and Tetrahymena tropicalis.

Tetramethyl Ammonium Chloride Agar Composition per liter: Agar ............................................................................................ 15.0g Tetramethyl ammonium chloride.................................................. 1.0g Thiamine·HCl ............................................................................... 0.5g Standard mineral base...................................................................1.0L pH 6.5 ± 0.2 at 25°C

Standard Mineral Base: Composition per liter: (NH4)2SO4 .................................................................................... 1.0g Phosphate buffer (1M solution, pH 6.8) ..................................40.0mL Huntner’s vitamin-free mineral base .......................................20.0mL

Huntner’s Vitamin-Free Mineral Base: Composition per liter: MgSO4·7H2O ............................................................................ 14.45g Nitrilotriacetic acid ..................................................................... 10.0g CaCl2·2H2O .............................................................................. 3.335g FeSO4·7H2O............................................................................. 99.0mg Metals “44”.............................................................................. 50.0mg (NH4)6Mo7O24·4H2O ............................................................... 9.25mg

Preparation of Huntner’s Vitamin Free Mineral Base: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Adjust pH to 7.0.

Metals “44”: Composition per 100.0mL: ZnSO4·7H2O ........................................................................ 1095.0mg FeSO4·7H2O........................................................................... 500.0mg Ethylenediaminetetraacetic acid ............................................ 250.0mg MnSO4·H2O ........................................................................... 154.0mg CuSO4·5H2O ............................................................................ 39.2mg Co(NO3)2·6H2O ....................................................................... 24.8mg Na 2B4O7·10H2O...................................................................... 17.7mg

Preparation of Metals “44”: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Paracoccus kocurii.

Tetramitus rostratus Flagellate Medium, PY/2 Composition per liter: Na2HPO4 ..................................................................................... 3.55g KH2PO4....................................................................................... 3.40g

Tetrathionate Broth

MgSO4·7H2O .............................................................................. 2.47g CaCl2, anhydrous ........................................................................ 1.12g Proteose peptone ........................................................................... 0.5g Yeast extract.................................................................................. 0.5g pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.5. Distribute into screw-capped tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Tetramitus rostratus.

Tetrathionate Brilliant Green HiVeg Broth Composition per liter: CaCO3 ......................................................................................... 20.0g K2S4O6 ........................................................................................ 20.0g Plant peptone................................................................................. 8.6g Synthetic detergent No. II ............................................................. 8.0g NaCl .............................................................................................. 6.4g Brilliant Green ............................................................................ 0.07g pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

1699

Tetrathionate Broth Base: Composition per liter: Na2S2O3·5H2O ............................................................................ 30.0g CaCO3 ......................................................................................... 10.0g Polypeptone™ .............................................................................. 5.0g Bile salts........................................................................................ 1.0g

Preparation of Tetrathionate Broth Base: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. A slight precipitate will remain. Do not autoclave. Cool to 25°C. Store at 4°C.

Iodine-Potassium Iodide Solution: Composition per 20.0mL: Iodine, resublimed ........................................................................ 6.0g KI .................................................................................................. 5.0g

Preparation of Iodine-Potassium Iodide Solution: Add KI to 5.0mL of sterile distilled/deionized water. Mix thoroughly. Add iodine. Mix thoroughly. Bring volume to 20.0mL with sterile distilled/deionized water. Brilliant Green Solution: Composition per 100.0mL: Brilliant Green .............................................................................. 0.1g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. A slight precipitate will remain. Do not autoclave. Cool to 25°C. Store at 4°C.

Use: For the enrichment and isolation of salmonellae.

Tetrathionate Broth Composition per liter: Na2S2O3 ...................................................................................... 40.7g CaCO3 ......................................................................................... 25.0g NaCl .............................................................................................. 4.5g Peptone.......................................................................................... 4.5g Yeast extract.................................................................................. 1.8g Beef extract ................................................................................... 0.9g Iodine solution .........................................................................20.0mL

Iodine Solution: Composition per 20.0mL: Iodine ............................................................................................ 6.0g KI .................................................................................................. 5.0g

Preparation of Iodine Solution: Add iodine and KI to distilled/

Preparation of Brilliant Green Solution: Add Brilliant Green to sterile distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Preparation of Medium: Combine 1.0L of tetrathionate broth base, 20.0mL of iodine-potassium iodide solution, and 10.0mL of Brilliant Green solution. Mix thoroughly. Aseptically distribute into tubes in 10.0mL volumes. Do not heat medium after it has been mixed. Use: For the selective isolation and cultivation of Salmonella species from foods.

Tetrathionate Broth (TT Broth) Composition per liter: Na2S2O3 ...................................................................................... 30.0g CaCO3 ......................................................................................... 10.0g Proteose peptone........................................................................... 5.0g Bile salts........................................................................................ 1.0g Iodine solution .........................................................................20.0mL pH 8.4± 0.2 at 25°C

deionized water and bring volume to 20.0mL. Mix thoroughly.

Source: This medium is available as a premixed powder from BD Di-

Preparation of Medium: Add components, except iodine solution,

agnostic Systems.

to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Do not autoclave. Cool to 40°C. Add 20.0mL of iodine solution. Mix thoroughly. Distribute into tubes in 10.0mL volumes. Use medium the same day it is prepared.

Iodine Solution: Composition per 20.0mL:

Use: For the selective isolation and enrichment of Salmonella typhi and

Preparation of Iodine Solution: Add iodine and KI to distilled/ deionized water and bring volume to 20.0mL. Mix thoroughly.

other salmonellae from fecal specimens, sewage, and other specimens.

Tetrathionate Broth (FDA M145) Composition per 1030.0mL: Tetrathionate broth base................................................................1.0L Iodine-potassium iodide solution.............................................20.0mL Brilliant Green solution ...........................................................10.0mL pH 8.4 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

Iodine ............................................................................................ 6.0g KI .................................................................................................. 5.0g

Preparation of Medium: Add components, except iodine solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Do not autoclave. Cool to 40°C. Add 20.0mL of iodine solution. Mix thoroughly. Distribute into tubes in 10.0mL volumes. Use medium the same day it is prepared.

Use: For the selective isolation and enrichment of Salmonella typhi and other salmonellae from infectious material.

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Tetrathionate Broth

Tetrathionate Broth (m-Tetrathionate Broth) (m-TT Broth) Composition per liter: Na2S2O3 ...................................................................................... 30.0g CaCO3 ......................................................................................... 10.0g Pancreatic digest of casein ............................................................ 2.5g Peptic digest of animal tissue........................................................ 2.5g Iodine–iodide solution .............................................................20.0mL pH 8.0 ± 0.2 at 25°C

Iodine-Iodide Solution: Composition per 20.0mL: Iodine ............................................................................................ 6.0g KI .................................................................................................. 5.0g

D-Mannitol .................................................................................... 2.5g Yeast extract.................................................................................. 2.0g Glucose ......................................................................................... 0.5g Sodium deoxycholate.................................................................... 0.5g Brilliant Green ............................................................................ 0.01g Iodine solution .........................................................................40.0mL pH 7.5–7.8 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Iodine Solution: Composition per 40.0mL: KI .................................................................................................. 8.0g Iodine ............................................................................................ 5.0g

Preparation of Iodine-Iodide Solution: Add iodine and KI to dis-

Preparation of Iodine Solution: Add iodine and KI to distilled/ deionized water and bring volume to 40.0mL. Mix thoroughly.

tilled/deionized water and bring volume to 20.0mL. Mix thoroughly.

Preparation of Medium: Add components, except iodine solution,

Preparation of Medium: Add components, except iodine-iodide

to distilled/deionized water and bring volume to 960.0mL. Mix thoroughly. Gently heat and bring to boiling. Do not autoclave. Cool to 40°C. Add 40.0mL of iodine solution. Mix thoroughly. Distribute into tubes in 10.0mL volumes. Use medium the same day it is prepared.

solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Do not autoclave. Cool to 40°C. Add 20.0mL of iodine-iodide solution. Mix thoroughly. Distribute into tubes in 10.0mL volumes. Use medium the same day it is prepared.

Use: For the selective isolation in the membrane filter method of Salmonella species from feces, urine, foods, and other specimens of sanitary importance.

Tetrathionate Broth (m-Tetrathionate Broth) Composition per liter: Na2S2O3 ...................................................................................... 30.0g Proteose peptone ........................................................................... 5.0g Bile salts........................................................................................ 1.0g Iodine solution .........................................................................20.0mL pH 8.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Iodine Solution: Composition per 20.0mL:

Use: For the isolation of Salmonella species, except Salmonella typhi, and Arizona species from fecal specimens, urine, food samples, and other specimens of sanitary significance.

Tetrathionate Broth with Novobiocin Composition per liter: Na2S2O3 ...................................................................................... 38.0g CaCO3 ......................................................................................... 25.0g Casein/meat peptone (50/50) ...................................................... 18.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 2.0g D-Mannitol .................................................................................... 0.5g Glucose ......................................................................................... 0.5g Sodium deoxycholate.................................................................... 0.5g Brilliant Green ............................................................................ 0.01g Novobiocin ................................................................................ 4.0mg Iodine solution .........................................................................40.0mL pH 7.5–7.8 at 25°C

Iodine Solution: Composition per 40.0mL:

Preparation of Iodine Solution: Add iodine and KI to distilled/

KI .................................................................................................. 8.0g Iodine ............................................................................................ 5.0g

deionized water and bring volume to 20.0mL. Mix thoroughly.

Use: For the enrichment of Salmonella species in the membrane filter method prior to placing the filter on selective media such as Brilliant Green broth.

Tetrathionate Broth, Hajna (TT Broth, Hajna) Composition per liter: Na2S2O3 ...................................................................................... 38.0g CaCO3 ......................................................................................... 25.0g Casein/meat peptone (50/50) ...................................................... 18.0g NaCl .............................................................................................. 5.0g © 2010 by Taylor and Francis Group, LLC

Preparation of Iodine Solution: Add iodine and KI to distilled/ deionized water and bring volume to 40.0mL. Mix thoroughly. Preparation of Medium: Add components, except iodine solution, to distilled/deionized water and bring volume to 960.0mL. Mix thoroughly. Gently heat and bring to boiling. Do not autoclave. Cool to 40°C. Add 40.0mL of iodine solution. Mix thoroughly. Distribute into tubes in 10.0mL volumes. Use medium the same day it is prepared.

Use: For the isolation of Salmonella species, except Salmonella typhi, and Arizona species from fecal specimens and other specimens of sanitary importance. Novobiocin suppresses the growth of Proteus species.

Tetrathionate Broth, USA (TT Broth, USA) Na2S2O3 ...................................................................................... 30.0g CaCO3 ......................................................................................... 10.0g

Tetrathionate Reductase Medium

Casein peptone .............................................................................. 2.5g Meat peptone................................................................................. 2.5g Bile salts........................................................................................ 1.0g Iodine-iodide solution ..............................................................20.0mL

Source: This medium is available as a premixed powder from Oxoid Unipath.

Iodine–Iodide Solution: Composition per 20.0mL: Iodine ............................................................................................ 6.0g KI .................................................................................................. 5.0g

Preparation of Iodine–Iodide Solution: Add iodine and KI to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly.

Use: For the selective enrichment of Salmonella species from feces, urine, foods, and other specimens of sanitary importance.

Tetrathionate Crystal Violet Enhancement Broth Composition per liter: Potassium tetrathionate ............................................................... 20.0g Casein/meat peptone (50/50) ........................................................ 8.6g NaCl .............................................................................................. 6.4g Crystal Violet ............................................................................ 0.005g pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the isolation of Salmonella species, except Salmonella typhi, and Arizona species from fecal specimens, urine, food samples, and other specimens of sanitary significance.

Tetrathionate HiVeg Broth Base, Hajna with Iodine (TT HiVeg Broth Base) Composition per liter: Na2S2O3 ...................................................................................... 38.0g CaCO3 ......................................................................................... 25.0g Plant special peptone .................................................................. 18.0g NaCl .............................................................................................. 5.0g D-Mannitol .................................................................................... 2.5g Yeast extract.................................................................................. 2.0g Glucose ......................................................................................... 0.5g Synthetic detergent No. III............................................................ 0.5g Brilliant Green ............................................................................ 0.01g Iodine solution .........................................................................40.0mL pH 7.6 ± 0.2 at 25°C

Source: This medium, without iodine, is available as a premixed powder from HiMedia. Iodine Solution: Composition per 40.0mL: KI .................................................................................................. 8.0g Iodine ............................................................................................ 5.0g © 2010 by Taylor and Francis Group, LLC

1701

Use: For the isolation of Salmonella species, except Salmonella typhi, and Arizona species from fecal specimens, urine, food samples, and other specimens of sanitary significance.

Tetrathionate Reductase Medium Composition per tube: Solution I .................................................................................10.0mL Solution III.................................................................................0.2mL Solution II ..................................................................................0.1mL Solution IV.................................................................................0.1mL

Solution I: Composition per liter: Na2HPO4·12H2O........................................................................... 3.6g KH2PO4......................................................................................... 1.0g NH4Cl ........................................................................................... 0.5g Peptone ....................................................................................... 0.25g Yeast extract................................................................................ 0.25g MgSO4·7H2O .............................................................................. 0.03g

Preparation of Solution I: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Solution II: Composition per 100.0mL: CaCl2·2H2O .................................................................................. 0.1g Ferric ammonium citrate............................................................. 0.05g

Preparation of Solution II: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Solution III: Composition per 100.0mL: Sodium succinate ........................................................................ 15.0g

Preparation of Solution III: Add sodium succinate to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Gently heat until dissolved. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Solution IV: Composition per 100.0mL: Na2S4O6·2H2O ............................................................................ 10.0g

Preparation of Solution IV: Add Na2S4O6·2H2O to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Sterilize by filtration. Store at 4°C. Preparation of Medium: To each tube containing 10.0mL of sterile solution I, aseptically add 0.1mL of sterile solution II, 0.2mL of sterile solution III, and 0.1mL of sterile solution IV. Mix thoroughly. Use immediately. Use: For the cultivation and differentiation of hydrogen-oxidizing bacteria based on their production of tetrathionate reductase.

1702

Tetrathionate Reductase Test Medium

Tetrathionate Reductase Test Medium Composition per 1025.0mL: K2S4O6 .......................................................................................... 5.0g Peptone water................................................................................1.0L Bromthymol Blue (0.2% solution)...........................................25.0mL pH 7.4 ± 0.2 at 25°C

Peptone Water: Composition per liter: Peptone........................................................................................ 10.0g NaCl .............................................................................................. 5.0g

Preparation of Peptone Water: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Combine components. Mix thoroughly. Adjust pH to 7.4. Filter sterilize. Dispense into tubes in 1.0mL volumes or into wells of sterile microculture plates for replica inoculation.

Use: For the cultivation and identification of Serratia species based on their ability to reduce tetrathionate. Bacteria that reduce tetrathionate turn the medium yellow.

Tetrazolium Thallium Glucose Agar Composition per liter: Agar ............................................................................................ 14.0g Beef extract ................................................................................. 10.0g Peptone........................................................................................ 10.0g Glucose solution ....................................................................100.0mL 2,3,5-Triphenyltetrazolium·HCl solution.................................10.0mL Thallous acetate solution .........................................................10.0mL

2,3,5-Triphenyltetrazolium·HCl Solution: Composition per 10.0mL: 2,3,5-Triphenyltetrazolium·HCl.................................................... 0.1g

Preparation of 2,3,5-Triphenyltetrazolium·HCl Solution: Add 2,3,5-triphenyltetrazolium·HCl to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 7 min at 15 psi pressure–121°C.

Glucose Solution: Composition per 100.0mL: Glucose ....................................................................................... 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Thallous Acetate Solution: Composition per 10.0mL: Thallous acetate ............................................................................ 1.0g

Preparation of Thallous Acetate Solution: Add thallous acetate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components—except glucose solution, 2,3,5-triphenyltetrazolium·HCl solution, and thallous acetate solution—to distilled/deionized water and bring volume to 880.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile glucose solution, 2,3,5-triphenyltetrazolium·HCl solution, and thallous acetate solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation of Streptococcus species. © 2010 by Taylor and Francis Group, LLC

Tetrazolium Tolerance Agar (TTC Agar) Composition per liter: Pancreatic digest of casein.......................................................... 15.0g Agar ............................................................................................ 15.0g Triphenyltetrazolium chloride .................................................... 10.0g Papaic digest of soybean meal...................................................... 5.0g NaCl.............................................................................................. 5.0g pH 7.3 ± 0.2 at 25°C

Use: For the differentiation of bacteria based upon the ability to tolerate and grow in the presence of tetrazolium. Streptococcus faecalis (enterococci) rapidly reduces tetrazolium.

TF Medium Composition per liter: NaCl.............................................................................................. 7.0g Pancreatic digest of casein............................................................ 2.0g Yeast extract.................................................................................. 2.0g MgSO4·7H2O ................................................................................ 1.8g K2HPO4......................................................................................... 1.6g MgCl2·6H2O ................................................................................. 1.4g Na2HPO4·H2O............................................................................... 1.0g NH4Cl ........................................................................................... 0.5g Na2S·9H2O.................................................................................... 0.3g MgSO4·7H2O .............................................................................. 0.16g Resazurin ................................................................................... 0.5mg Wolfe’s mineral solution..........................................................10.0mL Wolfe’s vitamin solution..........................................................10.0mL Glucose solution ......................................................................10.0mL CaCl2·2H2O solution................................................................10.0mL L-Cysteine·HCl·H2O solution ..................................................10.0mL pH 6.8–7.0 at 25°C

Wolfe’s Mineral Solution: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl.............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoCl2·6H2O .................................................................................. 0.1g ZnSO4·7H2O ................................................................................. 0.1g CaCl2·2H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O ............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g CuSO4·5H2O ............................................................................... 0.01g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O .......................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

TF(A) Medium

Wolfe’s Vitamin Solution: Composition per liter: Pyridoxine·HCl ........................................................................ 10.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Thiamine·HCl ............................................................................ 5.0mg Calcium DL-pantothenate........................................................... 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Wolfe’s Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Glucose Solution: Composition per 10.0mL: D-Glucose...................................................................................... 3.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

CaCl2·2H2O Solution: Composition per 10.0mL: CaCl2·2H2O................................................................................. 0.06g

Preparation of CaCl2·2H2O Solution: Add CaCl2·2H2O to dis-

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. L-Cysteine·HCl·H2O

Solution: Composition per 10.0mL:

L-Cysteine·HCl·H2O ..................................................................... 0.3g

Preparation of L-Cysteine·HCl·H2O Solution: Add L-cyste-

ine·HCl·H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Prepare and dispense medium under 100% N2. Add components, except glucose solution, CaCl2·2H2O solution, and L-cysteine·HCl·H2O solution, to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Adjust pH to 6.8–7.0. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add 10.0mL of sterile glucose solution, 10.0mL of sterile CaCl2·2H2O solution, and 10.0mL of sterile Lcysteine·HCl·H2O solution. Mix thoroughly. Final pH should be 6.8– 7.0.

Use: For the cultivation of Thermosipho species.

TF(A) Medium (DSMZ Medium 740) Composition per liter: K2HPO4 ......................................................................................... 1.6g Yeast extract.................................................................................. 2.0g Trypticase™.................................................................................. 2.0g Na2HPO4·H2O............................................................................... 1.0g NH4Cl ........................................................................................... 0.5g MgSO4·7H2O .............................................................................. 0.16g Resazurin ................................................................................... 0.5mg Calcium chloride solution ........................................................10.0mL Glucose solution ......................................................................10.0mL Cysteine solution......................................................................10.0mL © 2010 by Taylor and Francis Group, LLC

1703

Na2S·9H2O solution .................................................................10.0mL Trace elements solution ...........................................................10.0mL Vitamin solution.......................................................................10.0mL pH 6.8 ± 0.2 at 25°C

Calcium Chloride Solution: Composition per 10.0mL: CaCl2·2H2O ................................................................................ 0.06g

Preparation of Calcium Chloride Solution: Add CaCl2·2H2O

to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 25°C.

Cysteine Solution: Composition per 10.0mL: L-Cysteine·HCl·H2O ..................................................................... 0.3g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize. Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O.................................................................................... 0.3g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to distilled/

deionized water and bring volume to 10.0mL. Sparge with N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Store anaerobically.

Trace Elements Solution: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl.............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoSO4·7H2O............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

1704

TGA Medium

p-Aminobenzoic acid ................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Source: This medium is available as a premixed powder from BD Di-

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Use: For the enumeration of bacteria by the membrane filter method.

Preparation of Medium: Prepare and dispense medium under an

Composition per liter:

oxygen-free 100% N2. Add components, except vitamin solution, cysteine solution, calcium chloride solution, glucose solution, trace elements solution, and Na2S·9H2O solution, to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically and anaerobically add 10.0mL sterile vitamin solution, 10.0mL of sterile cysteine solution, 10.0mL sterile glucose solution, 10.0mL sterile calcium chloride solution, 10.0mL sterile trace elements solution, and 10.0mL of sterile Na2S·9H2O solution. Mix thoroughly. Adjust pH to 6.8. Aseptically and anaerobically distribute into sterile tubes or flasks.

Use: For the cultivation of Fervidobacterium pennivorans, Fervidobacterium gondwanense, and Fervidobacterium sp.

TGA Medium Composition per liter: Pancreatic digest of casein .......................................................... 10.0g NaCl .............................................................................................. 5.0g Glucose ......................................................................................... 2.0g pH 7.0 ± 0.2 at 25°C

Use: For the cultivation of Salmonella typhimurium.

TGB HiVeg Agar

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

TGMM-2 Medium Saccharomyces cerevisiae type II cells, dried................................................................... 3.0g Soy lecithin penterythritol mix ..................................................... 0.1g Skim milk powder...................................................................... 1.0mg

Source: Dried Saccharomyces cerevisiae type II cells and soy lecithin (type II-S L-α-phosphatidyl choline) are available from Sigma Chemical Co.

Soy Lecithin Penterythritol Mix: Composition per 0.11g: Penterythritol ................................................................................ 0.1g Soy lecithin (type II-S L-α-phosphatidyl choline) ................................................. 10.0mg

Preparation of Soy Lecithin Penterythritol Mix: Mix soy lecithin with the inert solid diluent penterythritol as a 1:10 mixture and grind to a fine powder.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat to 85°C while vigorously mixing for 10 min. Distribute 10.0mL volumes into tubes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Tetrahymena limacis, Tetrahymena patula, Tetrahymena rostrata, and Tetrahymena setosa.

TGY Medium (Tryptone Glucose Yeast Extract Medium)

Composition per liter of tap water: Agar ............................................................................................ 20.0g Pancreatic digest of casein............................................................ 5.0g Yeast extract.................................................................................. 5.0g Glucose ......................................................................................... 1.0g K2HPO4......................................................................................... 1.0g pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-

Preparation of Medium: Add components to distilled/deionized

Composition per liter:

Media.

Use: For the enumeration of bacteria in water, air, milk, and other dairy products.

TGE Broth

Use: For the cultivation and maintenance of a variety of bacteria, including Bacillus species, Corynebacterium species, Enterococcus species, and Pseudomonas species.

TGYM Medium (Tryptone Glucose Yeast Extract Methionine Medium)

Composition per liter:

Pancreatic digest of casein .......................................................... 10.0g Beef extract ................................................................................... 6.0g Glucose ......................................................................................... 2.0g pH 7.0 ± 0.2 at 25°C

Pancreatic digest of casein............................................................ 5.0g Yeast extract.................................................................................. 3.0g Glucose ......................................................................................... 1.0g DL-Methionine............................................................................... 0.5g

Thauera aromatica AR-1 Medium Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Deinococcus species.

Thauera aromatica AR-1 Medium (DSMZ Medium 855) Composition per 992.0mL:

1705

Solution E (Vitamin Solution): Composition per liter: Pyridoxine-HCl........................................................................ 10.0mg Thiamine-HCl·2H2O.................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg D-Ca-pantothenate ..................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 .............................................................................. 0.10mg

Solution A ..............................................................................870.0mL Solution C ..............................................................................100.0mL Solution D ................................................................................10.0mL Solution E (Vitamin solution) ..................................................10.0mL Solution B (Trace elements solution SL-10) .............................1.0mL Selenite-tungstate solution.........................................................1.0mL pH 7.2 ± 0.2 at 25°C

Solution E (Vitamin Solution): Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Solution A: Composition per 870.0mL:

Selenite-Tungstate Solution Composition per liter:

Na2SO4 .......................................................................................... 3.0g NaCl .............................................................................................. 1.0g KNO3 ............................................................................................ 0.6g KCl................................................................................................ 0.5g MgCl2·6H2O.................................................................................. 0.4g NH4Cl ........................................................................................... 0.3g KH2PO4 ......................................................................................... 0.2g CaCl2·2H2O................................................................................. 0.15g Resazurin ................................................................................... 1.0mg

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 870.0mL. Mix thoroughly. Solution B (Trace Elements Solution SL-10): Composition per liter: FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O........................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution) ..................................................................10.0mL

Solution C: Composition per 100.0mL: NaHCO3 ........................................................................................ 5.0g

Preparation of Solution C: Add NaHCO3 to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Flush with 80% N2 + 20% CO2 to remove dissolved oxygen.

Solution D: Composition per 10.0mL: Na-benzoate .................................................................................. 0.7g

Preparation of Solution D: Add Na-benzoate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. © 2010 by Taylor and Francis Group, LLC

NaOH............................................................................................ 0.5g Na2WO4·2H2O ........................................................................... 4.0mg Na2SeO3·5H2O........................................................................... 3.0mg

Preparation of Selenite-Tungstate Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Preparation of Medium: Gently heat solution A and bring to boiling. Boil solution A for a few minutes. Cool to room temperature. Gas with 80% N2 + 20% CO2 gas mixture to reach a pH below 6. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Sequentially add 1.0mL solution B, 100.0mL solution C, 10.0mL solution D, 10.0mL solution E, and 1.0mL sterile selenite-tungstate solution. Distribute anaerobically under 80% N2 + 20% CO2 into appropriate vessels. Addition of 10–20mg sodium dithionite per liter from a 5% (w/v) solution, freshly prepared under N2 and filter-sterilized, may stimulate growth.

Use: For the anaerobic cultivation of Thauera aromatica.

Thauera aromatica AR-1 Medium (DSMZ Medium 855) Composition per 892.0mL: Solution A..............................................................................870.0mL Solution C ................................................................................10.0mL Solution D (Vitamin solution) .................................................10.0mL Solution B (Trace elements solution SL-10) .............................1.0mL Selenite-tungstate solution.........................................................1.0mL pH 7.2 ± 0.2 at 25°C

Solution A: Composition per 870.0mL: NaCl.............................................................................................. 1.0g Na2SO4 .......................................................................................... 3.0g MgCl2·6H2O ................................................................................. 0.4g KH2PO4......................................................................................... 0.2g NH4Cl ........................................................................................... 0.3g KCl................................................................................................ 0.5g CaCl2·2H2O ................................................................................ 0.15g Resazurin ................................................................................... 1.0mg

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 870.0mL Mix thoroughly.

1706

Thauera aromatica Medium

Solution B (Trace Elements Solution SL-10): Composition per liter: FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O........................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution) ..................................................................10.0mL

Trace elements solution SL-10 ................................................10.0mL Vitamin solution.........................................................................5.0mL pH 7.5 ± 0.2 at 25°C

Solution A: Composition per 500.0mL: K2HPO4....................................................................................... 5.92g KH2PO4..................................................................................... 0.816g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 7.5. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Solution B: Composition per 500.0mL:

Solution C: Composition per 10.0mL:

KNO3 ............................................................................................ 2.0g Sodium benzoate......................................................................... 0.72g NH4Cl ....................................................................................... 0.267g MgSO4·7H2O ............................................................................ 0.197g CaCl2·2H2O .............................................................................. 0.025g

Na-benzoate .................................................................................. 0.7g

Preparation of Solution B: Add components to distilled/deionized

Preparation of Solution C: Add Na-benzoate to distilled/deion-

water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 7.5. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

ized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Solution D (Vitamin Solution): Composition per liter: Pyridoxine-HCl ........................................................................ 10.0mg Thiamine-HCl·2H2O .................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg D-Ca-pantothenate...................................................................... 5.0mg p-Aminobenzoic acid ................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 .............................................................................. 0.10mg

Trace Elements Solution SL-10: Composition per liter: FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

ionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Selenite-Tungstate Solution Composition per liter:

Vitamin Solution: Composition per liter:

Solution D (Vitamin Solution): Add components to distilled/de-

Preparation of Selenite-Tungstate Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize. Preparation of Medium: Adjust pH of solution A to 7.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Sequentially add 1.0mL solution B, 10.0mL solution C, 10.0mL solution D, and 1.0mL sterile selenite-tungstate solution. Adjust pH to 7.2. Aseptically distribute into tubes or flasks. Use: For the aerobic cultivation of Thauera aromatica.

Thauera aromatica Medium Composition 1015.0mL: Solution A ..............................................................................500.0mL Solution B ..............................................................................500.0mL © 2010 by Taylor and Francis Group, LLC

Pyridoxine·HCl ........................................................................ 10.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Thiamine·HCl ............................................................................ 5.0mg Calcium DL-pantothenate........................................................... 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Preparation of Medium: Aseptically combine 500.0mL of sterile solution A, 500.0mL of sterile solution B, 10.0 mL of sterile trace elements solution SL-10, and 5.0 mL of sterile vitamin solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Thauera aromatica.

Thayer-Martin HiVeg Medium Base with Hemoglobin and Vitox Supplement

Thauera mechernichi Medium (DSMZ Medium 918) Composition per liter: Na2HPO4 ..................................................................................... 4.20g Na-acetate ................................................................................... 2.93g KH2PO4 ......................................................................................... 1.5g NH4Cl ........................................................................................... 0.3g MgSO4·7H2O ................................................................................ 0.1g Trace elements solution .............................................................2.0mL pH 7.1 ± 0.2 at 25°C

1707

L-Cystine ....................................................................................... 1.1g

Adenine......................................................................................... 1.0g Nicotinamide adenine dinucleotide ............................................ 0.25g Vitamin B12 ................................................................................... 0.1g Thiamine pyrophosphate .............................................................. 0.1g Guanine·HCl ............................................................................... 0.03g Fe(NO3)3·6H2O........................................................................... 0.02g p-Aminobenzoic acid................................................................ 0.013g Thiamine·HCl ............................................................................ 3.0mg

Source: The supplement solution IsoVitaleX® enrichment is available

Trace Elements Solution: Composition per liter:

from BD Diagnostic Systems. This enrichment may be replaced by supplement VX from BD Diagnostic Systems.

Na2-EDTA................................................................................... 50.0g CaCl2·2H2O................................................................................... 5.5g MnCl2·4H2O................................................................................ 5.06g FeSO4·7H2O.................................................................................. 5.0g ZnSO4·7H2O ................................................................................. 2.0g CoCl2·6H2O ................................................................................ 1.61g CuSO4·5H2O ............................................................................... 1.57g (NH4)6Mo7O24·4H2O .................................................................... 1.1g

Preparation of Supplement Solution: Add components to dis-

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Thauera mechernichensis.

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except CNVT inhibitor and supplement solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile CNVT inhibitor and 10.0mL of sterile supplement solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation of Neisseria species from specimens containing mixed flora of bacteria and fungi.

Thayer-Martin HiVeg Medium Base with Hemoglobin and Vitox Supplement Composition per liter:

Thayer-Martin Agar, Modified (MTM II) (Modified Thayer-Martin Agar) Composition per liter: Agar ............................................................................................ 12.0g Hemoglobin ................................................................................ 10.0g Pancreatic digest of casein ............................................................ 7.5g Selected meat peptone .................................................................. 7.5g NaCl .............................................................................................. 5.0g K2HPO4 ......................................................................................... 4.0g Cornstarch ..................................................................................... 1.0g KH2PO4 ......................................................................................... 1.0g CNVT inhibitor........................................................................10.0mL Supplement solution ................................................................10.0mL pH 7.2 ± 0.2 at 25°C

CNVT Inhibitor: Composition per 10.0mL: Colistin sulfate ........................................................................... 7.5mg Trimethoprim lactate.................................................................. 5.0mg Vancomycin ............................................................................... 3.0mg Nystatin.................................................................................. 12,500U

Preparation of CNVT Inhibitor: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Supplement Solution: Composition per liter: Glucose ..................................................................................... 100.0g L-Cysteine·HCl............................................................................ 25.9g L-Glutamine................................................................................. 10.0g © 2010 by Taylor and Francis Group, LLC

Plant special peptone .................................................................. 23.0g Agar ............................................................................................ 13.0g NaCl.............................................................................................. 5.0g Starch ............................................................................................ 1.0g Hemoglobin solution .............................................................250.0mL Vitox supplement .....................................................................10.0mL pH 7.0 ± 0.2 at 25°C

Source: This medium, without hemoglobin or Vitox supplement, is available as a premixed powder from HiMedia. Hemoglobin Solution: Composition per 250.0mL: Hemoglobin .................................................................................. 5.0g

Preparation of Hemoglobin Solution: Add hemoglobin to distilled/deionized water and bring volume to 250.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Vitox Supplement: Composition per 10.0mL: Glucose ......................................................................................... 2.0g L-Cysteine·HCl ......................................................................... 0.518g L-Glutamine .................................................................................. 0.2g L-Cystine................................................................................... 0.022g Adenine sulfate ........................................................................... 0.01g Nicotinamide adenine dinucleotide ........................................... 5.0mg Cocarboxylase............................................................................ 2.0mg Guanine·HCl .............................................................................. 0.6mg Fe(NO3)3·6H2O.......................................................................... 0.4mg p-Aminobenzoic acid............................................................... 0.26mg Vitamin B12 ................................................................................ 0.2mg Thiamine·HCl .......................................................................... 0.06mg

1708

Thayer-Martin Medium

Preparation of Vitox Supplement: Add components to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except hemoblobin and Vitox supplement, to distilled/deionized water and bring volume to 740.0mL. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 250.0mL of sterile hemoglobin solution and 10.0mL of sterile Vitox supplement. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of fastidious microorganisms, especially Neisseria species. For the selective isolation of gonococci from pathological specimens.

Thayer-Martin Medium Composition per liter: GC agar base ..........................................................................740.0mL Hemoglobin solution..............................................................250.0mL Vitox supplement .....................................................................10.0mL pH 7.3 ± 0.2 at 25°C

GC Agar Base: Composition per 740.0mL: Special peptone ........................................................................... 15.0g Agar ............................................................................................ 10.0g NaCl .............................................................................................. 5.0g K2HPO4 ......................................................................................... 4.0g Cornstarch ..................................................................................... 1.0g KH2PO4 ......................................................................................... 1.0g pH 7.2 ± 0.2 at 25°C

Preparation of GC Agar Base: Add components of GC medium base and the hemoglobin to distilled/deionized water and bring volume to 740.0mL. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Hemoglobin Solution: Composition per 250.0mL: Hemoglobin .................................................................................. 5.0g

Preparation of Hemoglobin Solution: Add hemoglobin to distilled/deionized water and bring volume to 250.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Vitox Supplement: Composition per 10.0mL: Glucose ......................................................................................... 2.0g L-Cysteine·HCl.......................................................................... 0.518g L-Glutamine .................................................................................. 0.2g L-Cystine ................................................................................... 0.022g Adenine sulfate ........................................................................... 0.01g Nicotinamide adenine dinucleotide ........................................... 5.0mg Cocarboxylase............................................................................ 2.0mg Guanine·HCl .............................................................................. 0.6mg Fe(NO3)3·6H2O .......................................................................... 0.4mg p-Aminobenzoic acid ............................................................... 0.26mg Vitamin B12 ................................................................................ 0.2mg Thiamine·HCl .......................................................................... 0.06mg

Preparation of Vitox Supplement: Add components to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

10.0mL of sterile Vitox supplement. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of fastidious microorganisms, especially Neisseria species.

Thayer-Martin Medium Composition per liter: Hemoglobin ................................................................................ 10.0g GC medium base....................................................................980.0mL CNVT inhibitor........................................................................10.0mL Supplement B...........................................................................10.0mL pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a prepared medium in tubes from BD Diagnostic Systems.

GC Medium Base: Composition per 980.0mL: Proteose peptone No. 3 ............................................................... 15.0g Agar ............................................................................................ 10.0g NaCl.............................................................................................. 5.0g K2HPO4......................................................................................... 4.0g Cornstarch..................................................................................... 1.0g KH2PO4......................................................................................... 1.0g pH 7.2 ± 0.2 at 25°C

Preparation of GC Medium Base: Add components of GC medium base and the hemoglobin to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. CNVT Inhibitor: Composition per 10.0mL: Colistin sulfate ........................................................................... 7.5mg Trimethoprim lactate.................................................................. 5.0mg Vancomycin ............................................................................... 3.0mg Nystatin.................................................................................. 12,500U

Preparation of CNVT Inhibitor: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: To 980.0mL of cooled sterile GC medium base, aseptically add 10.0mL of sterile CNVT inhibitor and 10.0mL of sterile supplement B. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of fastidious microorganisms, especially Neisseria species.

Thayer-Martin Medium, Modified (Modified Thayer-Martin Agar) Composition per liter: GC agar base..........................................................................720.0mL Hemoglobin solution .............................................................250.0mL GC supplement ........................................................................30.0mL pH 7.3 ± 0.2 at 25°C

GC Agar Base: Composition per 720.0mL: Special peptone........................................................................... 15.0g Agar ............................................................................................ 10.0g NaCl.............................................................................................. 5.0g K2HPO4......................................................................................... 4.0g

Thayer-Martin Medium, Selective

1709

Cornstarch ..................................................................................... 1.0g KH2PO4 ......................................................................................... 1.0g pH 7.2 ± 0.2 at 25°C

Preparation of Hemoglobin Solution: Add hemoglobin to distilled/deionized water and bring volume to 250.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Preparation of GC Agar Base: Add components of GC medium

Vitox Supplement: Composition per 10.0mL:

base to distilled/deionized water and bring volume to 720.0mL. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Hemoglobin Solution: Composition per 250.0mL: Hemoglobin .................................................................................. 5.0g

Preparation of GC Supplement: Add components to distilled/deionized water and bring volume to 30.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: To 720.0mL of cooled sterile GC agar base, aseptically add 250.0mL of sterile hemoglobin solution and 30.0mL of sterile GC supplement. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation and cultivation of fastidious microorganisms, especially Neisseria species.

Thayer-Martin Medium, Modified (Modified Thayer-Martin Agar) Composition per liter: GC agar base ..........................................................................730.0mL Hemoglobin solution..............................................................250.0mL Vitox supplement .....................................................................10.0mL VCNT antibiotic solution.........................................................10.0mL pH 7.3 ± 0.2 at 25°C

GC Agar Base: Composition per 730.0mL: Special peptone ........................................................................... 15.0g Agar ............................................................................................ 10.0g NaCl .............................................................................................. 5.0g K2HPO4 ......................................................................................... 4.0g Cornstarch ..................................................................................... 1.0g KH2PO4 ......................................................................................... 1.0g pH 7.2 ± 0.2 at 25°C

Preparation of GC Agar Base: Add components of GC medium base in to distilled/deionized water and bring volume to 730.0mL. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Glucose ......................................................................................... 2.0g L-Cysteine·HCl......................................................................... 0.518g L-Glutamine.................................................................................. 0.2g L-Cystine .................................................................................. 0.022g Adenine sulfate ........................................................................... 0.01g Nicotinamide adenine dinucleotide ........................................... 5.0mg Cocarboxylase............................................................................ 2.0mg Guanine·HCl .............................................................................. 0.6mg Fe(NO3)3·6H2O.......................................................................... 0.4mg p-Aminobenzoic acid............................................................... 0.26mg Vitamin B12 ................................................................................ 0.2mg Thiamine·HCl .......................................................................... 0.06mg

Preparation of Vitox Supplement: Add components to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

VCNT Antibiotic Solution: Composition per 10.0mL: Colistin methane sulfonate ........................................................ 7.5mg Trimethoprim lactate.................................................................. 5.0mg Vancomycin ............................................................................... 3.0mg Nystatin.................................................................................. 12,500U

Preparation of VCNT Antibiotic Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: To 730.0mL of cooled, sterile GC agar base, aseptically add 250.0mL of sterile hemoglobin solution, 10.0mL of sterile Vitox supplement, and 10.0mL of VCNT antibiotic solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation and cultivation of fastidious microorganisms, especially Neisseria species.

Thayer-Martin Medium, Selective Composition per liter: GC agar base..........................................................................730.0mL Hemoglobin solution .............................................................250.0mL Vitox supplement .....................................................................10.0mL VCN antibiotic solution...........................................................10.0mL pH 7.3 ± 0.2 at 25°C

GC Agar Base: Composition per 730.0mL: Special peptone........................................................................... 15.0g Agar ............................................................................................ 10.0g NaCl.............................................................................................. 5.0g K2HPO4......................................................................................... 4.0g Cornstarch..................................................................................... 1.0g KH2PO4......................................................................................... 1.0g pH 7.2 ± 0.2 at 25°C

Preparation of GC Agar Base: Add components of GC medium base to distilled/deionized water and bring volume to 730.0mL. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

1710

Thayer-Martin Selective Agar

Hemoglobin Solution: Composition per 250.0mL:

L-Glutamine

Hemoglobin .................................................................................. 5.0g

Preparation of Hemoglobin Solution: Add hemoglobin to distilled/deionized water and bring volume to 250.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Vitox Supplement: Composition per 10.0mL: Glucose ......................................................................................... 2.0g L-Cysteine·HCl.......................................................................... 0.518g L-Glutamine .................................................................................. 0.2g L-Cystine ................................................................................... 0.022g Adenine sulfate ........................................................................... 0.01g Nicotinamide adenine dinucleotide ........................................... 5.0mg Cocarboxylase............................................................................ 2.0mg Guanine·HCl .............................................................................. 0.6mg Fe(NO3)3·6H2O .......................................................................... 0.4mg p-Aminobenzoic acid ............................................................... 0.26mg Vitamin B12 ................................................................................ 0.2mg Thiamine·HCl .......................................................................... 0.06mg

Preparation of Vitox Supplement: Add components to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

VCN Antibiotic Solution: Composition per 10.0mL: Colistin methane sulfonate......................................................... 7.5mg Vancomycin ............................................................................... 3.0mg Nystatin.................................................................................. 12,500U

Preparation of VCN Antibiotic Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: To 730.0mL of cooled, sterile GC agar base, aseptically add 250.0mL of sterile hemoglobin solution, 10.0mL of sterile Vitox supplement, and 10.0mL of VCN antibiotic solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation and cultivation of fastidious microorganisms, especially Neisseria species.

Thayer-Martin Selective Agar Composition per liter: Agar ............................................................................................ 12.0g Hemoglobin ................................................................................ 10.0g Pancreatic digest of casein ............................................................ 7.5g Selected meat peptone .................................................................. 7.5g NaCl .............................................................................................. 5.0g K2HPO4 ......................................................................................... 4.0g Cornstarch ..................................................................................... 1.0g KH2PO4 ......................................................................................... 1.0g Supplement solution ................................................................10.0mL VCN inhibitor ..........................................................................10.0mL pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems. Supplement Solution: Composition per liter: Glucose ..................................................................................... 100.0g L-Cysteine·HCl............................................................................ 25.9g © 2010 by Taylor and Francis Group, LLC

................................................................................ 10.0g

L-Cystine ....................................................................................... 1.1g

Source: The supplement solution IsoVitaleX® enrichment is available from BD Diagnostic Systems. This enrichment may be replaced by supplement VX from BD Diagnostic Systems.

Preparation of Supplement Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

VCN Inhibitor: Composition per 10.0mL: Colistin....................................................................................... 7.5mg Vancomycin ............................................................................... 3.0mg Nystatin.................................................................................. 12,500U

Preparation of VCN Inhibitor: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except supplement solution and VCN inhibitor, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile VCN inhibitor and sterile supplement solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation of Neisseria gonorrhoeae and Neisseria meningitidis from specimens containing mixed flora of bacteria and fungi.

Thermincola Medium (DSMZ Medium 1028) Composition per liter: NH4Cl .......................................................................................... 1.0g MgCl2·6H2O ............................................................................... 0.33g KH2PO4......................................................................................... 0.5g KCl.............................................................................................. 0.33g Na-acetate ..................................................................................... 0.2g CaCl2·2H2O .................................................................................. 0.1g Resazurin .................................................................................. 0.5mg Vitamin solution.......................................................................20.0mL Bicarbonate solution ................................................................10.0mL Carbonate solution ...................................................................10.0mL Sulfide solution........................................................................10.0mL Yeast extract solution...............................................................10.0mL Wolfe's mineral elixir.................................................................1.0mL pH 8.0 ± 0.2 at 25°C

Sulfide Solution: Composition per 10.0mL: Na2S·9H2O.................................................................................... 1.0g

Preparation of Sulfide Solution: Add Na2S·9H2O to distilled/de-

ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Thermoacetogenium phaeum Medium

Bicarbonate Solution: Composition per 10.0mL: NaHCO3 ........................................................................................ 0.5g

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with gas mixture of 80% N2 + 20% CO2. Filter sterilize.

Carbonate Solution: Composition per 10.0mL: Na2CO3 ......................................................................................... 0.5g

Preparation of Carbonate Solution: Add Na2CO3 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with gas mixture of 80% N2 + 20% CO2. Filter sterilize.

Yeast Extract Solution: Composition per 10.0mL: Yeast extract ................................................................................. 0.2g

Preparation of Yeast Extract Solution: Add yeast extract to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2 gas. Autoclave for 15 min at 15 psi pressure– 121°C. Wolfe’s Mineral Elixir: Composition per liter: MgSO4·7H2O .............................................................................. 30.0g NaCl ............................................................................................ 10.0g MnSO4·2H2O ................................................................................ 5.0g (NH4)2NiSO4·6H2O ...................................................................... 2.8g CoCl2·6H2O .................................................................................. 1.8g ZnSO4·7H2O ................................................................................. 1.8g FeSO4·7H2O.................................................................................. 1.0g CaCl2·2H2O................................................................................... 1.0g KAl(SO4)2·12H2O....................................................................... 0.18g CuSO4·5H2O ................................................................................. 0.1g H3BO3 ........................................................................................... 0.1g Na2MoO4·2H2O ............................................................................ 0.1g Na2SeO4 ........................................................................................ 0.1g Na2WO4·2H2O .............................................................................. 0.1g

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Culture vessels should be filled to approximately 20%. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aspetically add carbonate, bicarbonate, vitamin, and sulfide solutions. Mix thoroughly. Adjust the pH to 8.0 with sterile, anoxic 1N HCl. Prior to inoculation aseptically add yeast extract solution.

Use: For the cultivation of Thermincola carboxydiphila.

Thermoacetogenium phaeum Medium (DSMZ Medium 880) Composition per liter: KHCO3.......................................................................................... 3.5g NH4Cl ........................................................................................... 1.0g NaCl.............................................................................................. 0.6g KH2PO4......................................................................................... 0.3g MgCl2·6H2O ................................................................................. 0.1g CaCl2·2H2O ................................................................................ 0.08g Resazurin ................................................................................... 0.5mg Sodium pyruvate solution ........................................................50.0mL Vitamin solution.......................................................................10.0mL Na2S·9H2O solution .................................................................10.0mL Cysteine solution .....................................................................10.0mL Trace elements solution .............................................................1.0mL Selenite-tungstate solution.........................................................1.0mL pH 7.0–7.1 at 25°C

Sodium Pyruvate Solution: Composition per 50.0mL: Sodium pyruvate........................................................................... 5.0g

Preparation of Sodium Pyruvate Solution: Add sodium pyruvate to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize. Selenite-Tungstate Solution Composition per liter: NaOH............................................................................................ 0.5g Na2WO4·2H2O ........................................................................... 4.0mg Na2SeO3·5H2O........................................................................... 3.0mg

Preparation of Wolfe’s Mineral Elixir: Adjust pH of 1.0L of dis-

Preparation of Selenite-Tungstate Solution: Add components

tilled/deionized water to 1.0 with dilute H2SO4. Add components one at a time. Mix thoroughly to dissolve.

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Vitamin Solution: Composition per liter:

Na2S·9H2O Solution: Composition per 10.0mL:

Pyridoxine-HCl ........................................................................ 10.0mg Thiamine-HCl·2H2O .................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg D-Ca-pantothenate...................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Na2S·9H2O.................................................................................... 0.3g

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize.

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Cysteine Solution: Composition per 10.0mL: L-Cysteine·HCl·H2O ..................................................................... 0.3g

Preparation of Medium: Add components, except vitamin solu-

Trace Elements Solution SL-10: Composition per liter:

tion, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for 1 min. Cool to room temperature while sparging with 100% N2 gas. Dispense into culture vessels under an atmostphere of 100% CO (carbon monoxide) .

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Thermoacidurans Agar

ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution) ..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Adjust pH to 6.5. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Vitamin Solution: Composition per liter: Pyridoxine-HCl ........................................................................ 10.0mg Thiamine-HCl·2H2O .................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg D-Ca-pantothenate...................................................................... 5.0mg p-Aminobenzoic acid ................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize. Preparation of Medium: Prepare and dispense medium under 80% N2 + 20% CO2 gas atmosphere. Add components, except KHCO3, sodium pyruvate solution, cysteine solution, and Na2S·9H2O solution, to distilled/deionized water and bring volume to 930.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for 10 min. Cool to room temperature while sparging with 80% N2 + 20% CO2. Add 3.5g KHCO3. Mix thoroughly while sparging with 80% N2 + 20% CO2 gas atmosphere. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add 50.0mL sodium pyruvate solution, 10.0mL cysteine solution, and 10.0mL Na2S·9H2O solution. Mix thoroughly. Final pH is 7.0– 7.1. Aseptically and anaerobically distribute into sterile tubes or bottles.

Use: For the cultivation of Thermacetogenium phaeum.

Thermoacidurans Agar Composition per liter: Agar ............................................................................................ 20.0g Yeast extract.................................................................................. 5.0g Proteose peptone ........................................................................... 5.0g Glucose ......................................................................................... 5.0g K2HPO4 ......................................................................................... 4.0g pH 5.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Thermoacidurans HiVeg Agar Composition per liter: Agar ............................................................................................ 20.0g Glucose ......................................................................................... 5.0g Plant peptone No. 3....................................................................... 5.0g Yeast extract.................................................................................. 5.0g K2HPO4......................................................................................... 4.0g pH 5.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Use: For the isolation and cultivation of Bacillus thermoacidurans from food products.

Thermoactinomyces dichotomicus Medium Composition per liter: Maize, split ................................................................................. 50.0g Agar ............................................................................................ 20.0g Starch .......................................................................................... 10.0g NaCl.............................................................................................. 5.0g Peptone ......................................................................................... 5.0g CaCl2 ............................................................................................. 0.5g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add split maize (crushed corn) to 1.0L of boiling water. Steam for 30 min. Filter through Whatman #1 filter paper. Add remaining components to maize filtrate. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of Thermoactinomyces dichotomicus.

Thermoactinomyces Medium (DSMZ Medium 978) Composition per liter: Polypeptone™ ............................................................................ 30.0g Agar ............................................................................................ 15.0g Glycerol ........................................................................................ 2.0g L-Asparagine................................................................................. 1.0g K2HPO4......................................................................................... 1.0g Vitamin B solution...................................................................10.0mL Trace salts solution ....................................................................1.0mL pH 7.2 ± 0.2 at 25°C

Trace Salts Solution: Composition per 100.0mL: FeSO4·7H2O.................................................................................. 0.1g MnCl2·4H2O ................................................................................. 0.1g ZnSO4·7H2O ................................................................................. 0.1g

Preparation of Trace Salts Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Vitamin B Solution: Composition per 200.0mL: Thiamine-HCl .......................................................................... 10.0mg Riboflavin ................................................................................ 10.0mg Nicotinate................................................................................. 10.0mg Pyridoxine-HCl........................................................................ 10.0mg

Thermoanaerobacter ethanolicus Medium

Inositol ..................................................................................... 10.0mg Calcium pantothenate .............................................................. 10.0mg p-Aminobenzoate..................................................................... 10.0mg D-Biotin...................................................................................... 5.0mg

Preparation of Vitamin B Solution: Add components to distilled/ deionized water and bring volume to 200.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except vitamin B solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add 100.0mL vitamin B solution. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Thermoactinomyces peptonophilus.

Thermoactinomyces Medium Composition per liter:

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Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Preparation of Medium: Add components, except vitamin solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Adjust the pH to 7.2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aspetically add vitamin solution. Mix thoroughly. Aseptically dispense into culture vessels. Use: For the cultivation of Thermoactinomyces spp.

Thermoactinopolyspora Medium Composition per liter: Maltose ....................................................................................... 20.0g Agar ............................................................................................ 15.0g Papaic digest of soybean meal.................................................... 15.0g Yeast extract.................................................................................. 2.0g pH 7.2 ± 0.2 at 25°C

Agar ............................................................................................ 20.0g Malt extract ................................................................................. 10.0g Yeast extract.................................................................................. 4.0g Glucose ......................................................................................... 4.0g pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to tap water and bring

Preparation of Medium: Add components to distilled/deionized

Thermoactinopolyspora species.

Composition per liter:

Use: For the cultivation and maintenance of Thermoactinomyces sacchari.

Thermoactinomyces Medium (DSMZ Medium 978) Composition per liter: Polypeptone ................................................................................ 30.0g Agar ........................................................................................... 15.0g Glycerol ........................................................................................ 2.0g L-Asparagine ................................................................................. 1.0g KH2PO4 ......................................................................................... 1.0g Vitamin solution.......................................................................10.0mL Trace elements solution .............................................................1.0mL pH 7.2 ± 0.2 at 25°C

Vitamin Solution: Composition per 200.0mL: Pyridoxine-HCl ........................................................................ 10.0mg Thiamine-HCl·2H2O ................................................................ 10.0mg Riboflavin ................................................................................ 10.0mg Nicotinic acid ........................................................................... 10.0mg D-Ca-pantothenate.................................................................... 10.0mg Inositol ..................................................................................... 10.0mg p-Aminobenzoic acid............................................................... 10.0mg Biotin ......................................................................................... 5.0mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 200.0mL. Mix thoroughly. Filter sterilize. Trace Elements Solution: Composition per 100.0mL: FeSO4·7H2O.................................................................................. 0.1g MnCl2·4H2O.................................................................................. 0.1g ZnSO4·7H2O ................................................................................. 0.1g © 2010 by Taylor and Francis Group, LLC

volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Thermoactinomyces and

Thermoanaerobacter ethanolicus Medium Glucose ......................................................................................... 8.0g Na2HPO4·12H2O........................................................................... 4.2g Yeast extract.................................................................................. 2.0g KH2PO4......................................................................................... 1.5g NH4Cl ........................................................................................... 0.5g MgCl2·6H2O ............................................................................... 0.18g Reducing solution ....................................................................40.0mL Wolfe’s modified mineral solution ............................................5.0mL Resazurin (0.1% solution) .........................................................1.0mL Vitamin solution.........................................................................0.5mL

Caution: This medium contains Na2S, and H2S production will occur, especially upon prolonged boiling. H2S is hazardous and preparation of this medium should be done in a chemical fume hood. Reducing Solution: Composition per 200.0mL: Cysteine·HCl·H2O ........................................................................ 2.5g Na2S·9H2O.................................................................................... 2.5g NaOH (0.2N solution)............................................................200.0mL

Preparation of Reducing Solution: Gently heat the NaOH solution and bring to boiling. Gas with 95% N2 + 5% H2. Cool to room temperature. Add the cysteine·HCl·H2O and Na2S·9H2O. Anaerobically distribute into tubes. Cap with rubber stoppers. Autoclave for 15 min at 15 psi pressure–121°C.

Vitamin Solution: Composition per 500.0mL: Pyridoxine·HCl ............................................................................. 0.1g p-Aminobenzoic acid.................................................................. 0.05g Calcium pantothenate ................................................................. 0.05g Nicotinic acid.............................................................................. 0.05g Thioctic acid ............................................................................... 0.05g Biotin .......................................................................................... 0.02g Folic acid .................................................................................... 0.02g Riboflavin .................................................................................. 5.0mg

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Thermoanaerobacter subterraneus Medium

Thiamine·HCl ............................................................................ 5.0mg Vitamin B12 ................................................................................ 1.0mg

Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 500.0mL. Mix thoroughly. Store solution in the dark at −10°C.

NaHCO3 Solution: Composition per 20.0mL:

Wolfe’s Modified Mineral Solution: Composition per liter:

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Filter sterilize.

MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl .............................................................................................. 1.0g MnSO4·H2O .................................................................................. 0.5g CaCl2 (anhydrous)......................................................................... 0.1g Co(NO3)2·6H2O ............................................................................ 0.1g FeSO4·7H2O.................................................................................. 0.1g ZnSO4·7H2O ................................................................................. 0.1g AlK(SO4)2 (anhydrous)............................................................... 0.01g CuSO4·5H2O ............................................................................... 0.01g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O .......................................................................... 0.01g Na2SeO3 (anhydrous)................................................................. 1.0mg

Preparation of Wolfe’s Modified Mineral Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L.

Preparation of Medium: Add components, except reducing solution, to distilled/deionized water and bring volume to 1.0L. Gently heat and bring to boiling under 95% N2 + 5% H2. Continue boiling until color changes from blue to pink. Add the reducing solution. The pink color will disappear, indicating that the solution has been reduced. Distribute into tubes or flasks under 95% N2 + 5% H2 using anerobic techniques. Cap tubes with rubber stoppers. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of thermophilic anaerobes such as Thermoanaerobacter species and some Clostridium species.

Thermoanaerobacter subterraneus Medium (DSMZ Medium 899) Composition per liter: Yeast extract.................................................................................. 2.0g MgCl2·6H2O.................................................................................. 1.0g NH4Cl ........................................................................................... 1.0g NaCl .............................................................................................. 0.6g Cysteine-HCl·H2O ........................................................................ 0.5g K2HPO4 ......................................................................................... 0.3g KH2PO4 ......................................................................................... 0.3g KCl................................................................................................ 0.2g CaCl2·2H2O................................................................................... 0.1g Resazurin ................................................................................... 0.5mg D-Glucose solution...................................................................30.0mL NaHCO3 solution .....................................................................20.0mL Trace mineral solution .............................................................10.0mL Na2S2O3 solution......................................................................10.0mL Na2S·9H2O solution .................................................................10.0mL pH 7.0 ± 0.2 at 25°C

Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................. 0.45g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

NaHCO3 ........................................................................................ 4.0g

Glucose Solution: Composition per 30.0mL: D-Glucose...................................................................................... 4.0g

Preparation of Glucose Solution: Add D-glucose to distilled/deionized water and bring volume to 30.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Na2S2O3 Solution: Composition per 10.0mL: Na2S2O3·5H2O .............................................................................. 2.5g

Preparation of Na2S2O3 Solution: Add Na2S2O3·5H2O to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Mix thoroughly. Preparation of Medium: Prepare and dispense medium under 80% N2 + 20% CO2 gas atmosphere. Add components, except NaHCO3 solution, glucose solution, Na2S·9H2O solution, and Na2S2O3 solution, to distilled/deionized water and bring volume to 930.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Distribute into sterile tubes or bottles. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically per 1.0L of medium add 20.0mL NaHCO3 solution, 30.0mL glucose solution, 10.0mL Na2S·9H2O solution, and 10.0mL Na2S2O3 solution. Mix thoroughly. The final pH should be 7.0.

Use: For the cultivation of Thermoanaerobacter subterraneus.

Thermoanaerobacter sulfurophilus Medium (DSMZ Medium 827) Composition per 1055.0mL: Sulfur, powdered......................................................................... 10.0g NH4Cl ......................................................................................... 0.33g

Thermoanaerobacter tengcongensis Medium

KCl.............................................................................................. 0.33g KH2PO4 ....................................................................................... 0.33g MgCl2·6H2O................................................................................ 0.33g CaCl2·2H2O................................................................................. 0.33g Resazurin ................................................................................... 0.5mg Glucose solution ......................................................................25.0mL Na2S·9H2O solution .................................................................15.0mL Vitamin solution.......................................................................10.0mL Yeast extract solution .................................................................5.0mL Trace elements solution SL-10 ..................................................1.0mL pH 7.0 ± 0.2 at 25°C

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize. Glucose Solution: Composition per 50.0mL: Glucose ....................................................................................... 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize. Yeast Extract Solution: Composition per 10.0mL: Yeast extract.................................................................................. 1.0g

Preparation of Yeast Extract Solution: Add yeast extract to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

1715

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature. Preparation of Medium: Prepare and dispense medium under 80% N2 + 20% CO2 gas mixture. Add components, except Na2S·9H2O solution, glucose solution, vitamin solution, and yeast extract solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Heat to 90°C on each of 3 successive days. Aseptically and anaerobically add 25.0mL sterile glucose solution, 15.0mL sterile Na2S·9H2O solution, 10.0mL sterile vitamin solution, and 5.0mL sterile yeast extract solution. Mix thoroughly. Aseptically and anaerobically distribute to tubes or bottles. The pH should be 7.0.

Use: For the cultivation of Thermoanaerobacter sulfurophilus.

Thermoanaerobacter tengcongensis Medium (DSMZ Medium 965) Composition per liter: Soluble starch.............................................................................. 10.0g NaCl.............................................................................................. 2.0g Tryptone........................................................................................ 2.0g NH4Cl ........................................................................................... 1.0g Yeast extract.................................................................................. 1.0g MgCl2·6H2O ................................................................................. 0.5g Cysteine-HCl·H2O ........................................................................ 0.5g K2HPO4......................................................................................... 0.3g KH2PO4......................................................................................... 0.3g KCl................................................................................................ 0.2g CaCl2·2H2O ................................................................................ 0.05g Resazurin ................................................................................... 0.5mg Thiosulfate solution .................................................................50.0mL Trace elements solution ...........................................................10.0mL pH 7.5 ± 0.2 at 25°C

Thiosulfate Solution: Composition per 50.0mL: Na2S2O3·5H2O .............................................................................. 5.0g

Preparation of Thiosulfate Solution: Add Na2S2O3·5H2O to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Trace Elements Solution: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl.............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoSO4·7H2O............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH

1716

Thermoanaerobacterium Medium

to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Mix thoroughly.

Selenite Solution: Composition per liter:

Preparation of Medium: Add components, except thiosulfate so-

NaOH............................................................................................ 0.5g Na2SeO3·5H2O........................................................................... 3.0mg

lution, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for 3 min. Cool to room temperature while sparging with 100% N2 gas. Adjust pH to 7.5. Distribute into tubes or bottles under 100% N2 gas. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically and anaerobically under 100% N2 gas, add 50.0mL sterile thiosulfate solution per liter of medium.

Use: For the cultivation of Thermoanaerobacter tengcongensis.

Thermoanaerobacterium Medium (DSMZ Medium 903) Composition per 1030.0mL: KH2PO4 ......................................................................................... 0.5g NaCl .............................................................................................. 0.4g MgCl2·6H2O................................................................................ 0.33g Trypticase™................................................................................ 0.25g CaCl2·2H2O................................................................................. 0.05g Resazurin ................................................................................... 0.5mg Sucrose solution .......................................................................50.0mL NaHCO3 solution .....................................................................20.0mL Na2S·9H2O solution .................................................................10.0mL L-Cysteine solution ..................................................................10.0mL Selenite solution.........................................................................1.0mL Seven vitamin solution...............................................................1.0mL Trace elements solution SL-10 ..................................................1.0mL pH 7.0 ± 0.2 at 25°C Sucrose Solution: Composition per 50.0mL: Sucrose.......................................................................................... 5.0g

Preparation of Sucrose Solution: Add sucrose to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Selenite Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize. Seven Vitamin Solution: Composition per liter: Pyridoxine hydrochloride ...................................................... 300.0mg Thiamine-HCl·2H2O.............................................................. 200.0mg Nicotinic acid......................................................................... 200.0mg Vitamin B12 ............................................................................ 100.0mg Calcium pantothenate ............................................................ 100.0mg p-Aminobenzoic acid............................................................... 80.0mg D(+)-Biotin............................................................................... 20.0mg

Preparation of Seven Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Sparge with 100% N2. Mix thoroughly. Filter sterilize. Trace Elements Solution SL-10: Composition per liter: FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure– 121°C.

Preparation of Medium: Prepare and dispense medium under 80% N2 + 20% CO2. Add components, except seven vitamin solution, NaHCO3 solution, sucrose solution, L-cysteine-HCl·H2O solution, and Na2S·9H2O solution, to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Adjust pH to 7.0. Distribute into anaerobe tubes or bottles. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add, per liter, 10.0mL seven vitamin solution, 20.0mL NaHCO3 solution, 50.0mL sucrose solution, 10.0mL Lcysteine-HCl·H2O solution, and 10.0mL Na2S·9H2O solution. Mix thoroughly. The final pH should be 7.0.

Na2S·9H2O Solution: Composition per 10.0mL:

Use: For the cultivation of Thermoanaerobacterium polysaccharolyticum and Thermoanaerobacterium zeae.

L-Cysteine

Solution: Composition per 10.0mL:

L-Cysteine·HCl·H2O ..................................................................... 0.3g

Preparation of L-Cysteine Solution: Add L-cysteine·HCl·H2O to

Na2S·9H2O .................................................................................... 0.3g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

NaHCO3 Solution: Composition per 20.0mL: NaHCO3 ........................................................................................ 2.5g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 25°C. Must be prepared freshly. © 2010 by Taylor and Francis Group, LLC

Thermoanaerobium brockii Medium Composition per liter: Pancreatic digest of casein.......................................................... 10.0g Yeast extract.................................................................................. 3.0g K2HPO4......................................................................................... 1.5g NH4Cl ........................................................................................... 0.9g NaCl.............................................................................................. 0.9g KH2PO4....................................................................................... 0.75g MgCl2·6H2O ................................................................................. 0.2g Glucose solution ......................................................................25.0mL Na2S·9H2O (10% solution) ......................................................10.0mL

Thermoanaeromonas Medium

Trace elements solution .............................................................9.0mL Wolfe’s Vitamin solution ...........................................................5.0mL Resazurin (0.025% solution)......................................................4.0mL FeSO4·7H2O (10% solution)....................................................0.03mL pH 7.3 ± 0.2 at 25°C

Glucose Solution: Composition per 100.0mL: Glucose ....................................................................................... 20.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Trace Elements Solution: Composition per liter: Nitrilotriacetic acid ..................................................................... 12.5g NaCl .............................................................................................. 1.0g FeCl3·4H2O ................................................................................... 0.2g MnCl2·4H2O.................................................................................. 0.1g CaCl2·2H2O................................................................................... 0.1g ZnCl2 ............................................................................................. 0.1g CuCl2........................................................................................... 0.02g Na2SeO3 ...................................................................................... 0.02g CoCl2·6H2O .............................................................................. 0.017g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O .......................................................................... 0.01g

Preparation of Trace Elements Solution: Add nitrilotriacetic

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Glucose ......................................................................................... 0.5g Yeast extract.................................................................................. 0.5g MgSO4·7H2O .............................................................................. 0.25g NaCl.............................................................................................. 0.2g Na3-EDTA................................................................................... 0.04g CaCl2·2H2O ................................................................................ 0.03g FeSO4·7H2O................................................................................ 0.01g Resazurin ................................................................................... 0.5mg Thiosulfate solution .................................................................20.0mL Vitamin solution.......................................................................10.0mL Trace elements solution ...........................................................10.0mL Cysteine solution .....................................................................10.0mL pH 6.5 ± 0.2 at 25°C

Vitamin Solution: Composition per liter: Pyridoxine-HCl........................................................................ 10.0mg Thiamine-HCl·2H2O.................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg D-Ca-pantothenate ..................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L.

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize.

Wolfe’s Vitamin Solution: Composition per liter:

Trace Elements Solution: Composition per liter:

Pyridoxine·HCl ........................................................................ 10.0mg Thiamine·HCl ............................................................................ 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg Calcium pantothenate ................................................................ 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Thioctic acid .............................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Cyanocobalamin .....................................................................100.0μg

Preparation of Wolfe’s Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, except glucose solution, to distilled/deionized water and bring volume to 975.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. While still hot, aseptically add 25.0mL of the sterile glucose solution under 97% N2 + 3% H2. Adjust pH to 7.3 if necessary. Aseptically and anaerobically distribute into tubes. Cap with rubber stoppers. Use: For the cultivation and maintenance of Thermoanaerobium brockii.

MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl.............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoSO4·7H2O............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Cysteine Solution: Composition per 10.0mL: L-Cysteine·HCl·H2O ................................................................... 0.25g

Thermoanaeromonas Medium (DSMZ Medium 963) Composition per liter: NaHCO3 ........................................................................................ 5.0g K2HPO4 ....................................................................................... 0.78g KH2PO4 ....................................................................................... 0.75g NH4Cl ........................................................................................... 0.5g © 2010 by Taylor and Francis Group, LLC

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Thermoanaerovibrio Medium

Preparation of Thiosulfate Solution: Add Na2S2O3·5H2O to dis-

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Preparation of Medium: Add components, except NaHCO3, vita-

Na2S·9H2O Solution: Composition per 10.0mL:

tilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

min solution, thiosulfate solution, and cysteine solution, to distilled/deionized water and bring volume to 960.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for 3 min. Cool to 25°C while sparging with 80% N2 + 20% CO2. Add solid NaHCO3. Adjust pH to 6.8–7.0. Distribute to anaerobe tubes or bottles under 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add, per liter of medium, 10.0mL sterile vitamin solution, 10.0mL sterile cysteine solution, and 20.0mL sterile thiosulfate solution. Mix thoroughly. The final pH should be 6.5.

Use: For the cultivation of Thermanaeromonas toyohensis.

Thermoanaerovibrio Medium (DSMZ Medium 873) Composition per liter: NaHCO3 ........................................................................................ 0.8g NH4Cl ......................................................................................... 0.33g KH2PO4 ....................................................................................... 0.33g MgCl2·6H2O................................................................................ 0.33g CaCl2·2H2O................................................................................. 0.22g KCl.............................................................................................. 0.33g Yeast extract................................................................................ 0.25g Peptone........................................................................................ 0.25g Resazurin ................................................................................... 0.5mg NaHCO3 solution .....................................................................20.0mL Na2S·9H2O solution .................................................................10.0mL Vitamin solution.......................................................................10.0mL Glucose solution ......................................................................10.0mL Calcium chloride solution ........................................................10.0mL Magnesium chloride solution...................................................10.0mL Trace elements solution SL-10 ..................................................1.0mL pH 7.0–7.3 at 25°C

NaHCO3 Solution: Composition per 20.0mL: NaHCO3 ........................................................................................ 2.0g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Filter sterilize. Magnesium Chloride Solution: Composition per 10.0mL: MgCl2·6H2O................................................................................ 0.33g

Preparation of Magnesium Chloride Solution: Add 0.33g of MgCl2·6H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Calcium Chloride Solution: Composition per 10.0mL: CaCl2·2H2O................................................................................. 0.22g

Preparation of Calcium Chloride Solution: Add CaCl2·2H2O

to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Na2S·9H2O.................................................................................... 0.5g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Before use, neutralize to pH 7.0 with sterile HCl. Vitamin Solution: Composition per liter: Pyridoxine-HCl........................................................................ 10.0mg Thiamine-HCl·2H2O.................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg D-Ca-pantothenate ..................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

Preparation of Medium: Prepare and dispense medium under 80% N2 + 20% CO2 gas atmosphere. Add components, except NaHCO3 solution, glucose solution, calcium chloride solution, magnesium chloride solution, Na2S·9H2O solution, vitamin solution, and trace elements solution SL-10, to distilled/deionized water and bring volume to 929.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add 10.0mL glucose solution, 10.0mL Na2S·9H2O solution, 10.0mL magnesium chloride solution, 10.0ml calcium chloride solution, 10.0mL vitamin solution, and 1.0mL trace elements solution SL-10. Mix thoroughly. Adjust pH to 7.0–7.3 with 10.0mL NaHCO3 solution. Aseptically and anaerobically distribute into sterile tubes or bottles.

Use: For the cultivation of Thermanaerovibrio velox (Thermosinus velox).

Thermobacteroides proteolyticus Medium

Thermobacterium Medium Composition per liter: Agar ............................................................................................ 20.0g (NH4)2SO4 ..................................................................................... 1.3g Yeast extract.................................................................................. 1.0g Pancreatic digest of casein ............................................................ 1.0g KH2PO4 ....................................................................................... 0.28g MgSO4·7H2O ............................................................................ 0.247g CaCl2·2H2O............................................................................... 0.074g FeCl3·6H2O ............................................................................... 0.019g Salt solution ...............................................................................1.0mL pH 8.5 + 0.2 at 25°C

Salt Solution: Composition per liter: Na2B4O7·10H2O............................................................................ 4.4g MnCl2·4H2O.................................................................................. 1.8g ZnSO4·7H2O ............................................................................... 0.22g CuCl2·H2O .................................................................................. 0.05g Na2MoO4·2H2O........................................................................... 0.03g VOSO4·2H2O .............................................................................. 0.03g

Preparation of Salt Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 2.0 with H2SO4.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 11.0–12.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to solidify in a slanted position.

Use: For the cultivation and maintenance of Thermomicrobium roseum.

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min. Add the Na2S. Mix thoroughly. Sparge with 100% N2 for 10 min. Autoclave for 15 min at 15 psi pressure–121°C.

Solution A: Composition per liter: NH4Cl ....................................................................................... 100.0g MgCl2·H2O .............................................................................. 100.0g CaCl2·2H2O ............................................................................... 40.0g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 4 with HCl.

Mineral Salts Solution: Composition per liter: EDTA·2H2O.................................................................................. 0.5g CoCl2·H2O .................................................................................. 0.15g MnCl2·4H2O ................................................................................ 0.1g FeSO4·7H2O.................................................................................. 0.1g ZnCl2 ............................................................................................ 0.1g AlCl3·H2O............................................................................... 40.0mg Na2WO4·2H2O ........................................................................ 30.0mg CuCl2·2H2O ............................................................................ 20.0mg NiSO4·H2O.............................................................................. 20.0mg H2SeO3 .................................................................................... 10.0mg H3BO4 ...................................................................................... 10.0mg NaMoO4·2H2O........................................................................ 10.0mg

Preparation of Mineral Salts Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3 with HCl.

Solution B: Composition per liter: K2HPO4·3H2O ......................................................................... 200.0g

Thermobacteroides leptospartum Medium Composition per 1168.1mL: Yeast extract.................................................................................. 2.0g Trypticase™.................................................................................. 2.0g NaOH solution ..............................................................................1.0L Glucose solution ....................................................................113.0mL Na2S solution ...........................................................................22.6mL Solution A ................................................................................10.0mL Mineral salts solution...............................................................10.0mL Solution B ..................................................................................2.0mL Resazurin solution......................................................................0.5mL

NaOH Solution: Composition per liter: NaOH ........................................................................................... 4.0g

Preparation of NaOH Solution: Add NaOH to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Glucose Solution: Composition per 100.0mL: D-Glucose...................................................................................... 5.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Sparge with 100% N2 for 15 min. Autoclave for 15 min at 15 psi pressure–121°C.

Na2S Solution:

Na2S ............................................................................................. 2.5g

Preparation of Solution B: Add K2HPO4·3H2O to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Resazurin Solution: Composition per 100.0mL: Resazurin ...................................................................................... 0.2g

Preparation of Resazurin Solution: Add resazurin to distilled/ deionized water and bring volume to 100.0mL. Mix thoroughly. Preparation of Medium: Sparge 1.0L of NaOH solution with 100% CO2 for 30 min. Add 2.0g of yeast extract and 2.0g of Trypticase™. Mix thoroughly. Add 10.0mL of solution A, 2.0mL of solution B, 0.5mL of resazurin solution, and 10.0mL of mineral salts solution with pipets which have been flushed a few times with 100% N2. Mix thoroughly. Anaerobically distribute 9.0mL volumes into anaerobic tubes fitted with butyl rubber stoppers. Autoclave for 15 min at 15 psi pressure–121°C. One hour prior to inoculation, add 1.0mL of sterile glucose solution and 0.2mL of sterile Na2S solution to each 9.0mL of medium.

Use: For the cultivation of Thermobacteroides leptospartum.

Thermobacteroides proteolyticus Medium Composition per 1010.0mL: NaHCO3 ........................................................................................ 5.0g Pancreatic digest of casein............................................................ 2.0g Yeast extract.................................................................................. 2.0g MgCl2·6H2O ................................................................................. 1.0g NH4Cl ........................................................................................... 1.0g CaCl2·2H2O .................................................................................. 0.4g

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Thermococcus celer Medium

K2HPO4 ......................................................................................... 0.4g Na2S·9H2O .................................................................................... 0.3g Resazurin ................................................................................... 1.0mg Trace elements solution ...........................................................10.0mL pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components, except Na2S·9H2O solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.8. Do not autoclave. Sterilize by steaming at 100°C for 30 min on 3 consecutive days. Before inoculation, add 10.0mL of sterile Na2S·9H2O solution. Mix thoroughly.

Trace Elements Solution: Composition per liter:

Use: For the cultivation of Thermococcus celer.

MgSO4·7H2O ............................................................................... 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl .............................................................................................. 1.0g MnSO4·2H2O ............................................................................... 0.5g CoSO4·7H2O ............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O................................................................................... 0.1g FeSO4·7H2O.................................................................................. 0.1g KAI(SO4)2·12H2O....................................................................... 0.02g CuSO4·5H2O ............................................................................... 0.01g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O ......................................................................... 0.01g NiCl2·6H2O ............................................................................... 0.025g Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L.

Preparation of Medium: Prepare and dispense medium under 80% N2 + 20% CO2. Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Thermobacteroides proteolyticus.

Thermococcus celer Medium Composition per liter: NaCl ............................................................................................ 40.0g Sulfur .......................................................................................... 10.0g Yeast extract.................................................................................. 2.0g (NH4)2SO4 ..................................................................................... 1.3g KH2PO4 ....................................................................................... 0.28g MgSO4·7H2O .............................................................................. 0.25g CaCl2·2H2O................................................................................. 0.07g FeCl2·2H2O ................................................................................. 0.02g NaB4O·10H2O............................................................................ 4.5mg MnCl2·4H2O............................................................................... 1.8mg Resazurin ................................................................................... 1.0mg ZnSO4·7H2O ............................................................................ 0.22mg CuCl2·2H2O ............................................................................. 0.05mg NaMoO4·2H2O......................................................................... 0.03mg VOSO4·2H2O ........................................................................... 0.03mg CoSO4 ...................................................................................... 0.01mg Na2S·9H2O solution .................................................................10.0mL pH 5.8 ± 0.2 at 25°C

Na2S·9H2O Solution: Composition per 10.0mL:

Thermococcus celer Medium Composition per liter: NaCl............................................................................................ 40.0g Sulfur, powdered........................................................................... 5.0g (NH4)2SO4 .................................................................................... 1.3g KH2PO4....................................................................................... 0.28g MgSO4·7H2O .............................................................................. 0.25g CaCl2·2H2O ................................................................................ 0.07g CuCl2·2H2O ................................................................................ 0.05g FeCl3·6H2O ................................................................................. 0.02g Na2B4O7·10H2O......................................................................... 4.5mg MnCl2·4H2O .............................................................................. 1.8mg Resazurin ................................................................................... 1.0mg ZnSO4·7H2O ............................................................................ 0.22mg Na2MoO4·2H2O ....................................................................... 0.03mg VOSO4·2H2O........................................................................... 0.03mg CoSO4·7H2O ............................................................................ 0.01mg Yeast extract solution.................................................................... 2.0g Na2S·9H2O solution .................................................................10.0mL pH 5.8 ± 0.2 at 25°C

Preparation of Sulfur: Add 10.0g of powdered sulfur to a flask and sterilize by steaming for 3 hr on 3 consecutive days.

Yeast Extract Solution: Composition per 20.0mL: Yeast extract.................................................................................. 2.0g

Preparation of Yeast Extract Solution: Add yeast extract to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for a few minutes. Do not autoclave. Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O.................................................................................... 0.3g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Preparation of Medium: Prepare and dispense medium under 100% N2. Add components, except sulfur, yeast extract solution, and Na2S·9H2O solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 5.0g of sterile sulfur, 10.0mL of sterile yeast extract solution, and 10.0mL of sterile Na2S·9H2O solution. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or flasks.

Use: For the cultivation of Thermococcus celer.

Thermococcus chitinophagus Medium (DSMZ Medium 766)

Na2S·9H2O .................................................................................... 0.3g

Composition per liter:

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

Chitin, purified.............................................................................. 4.0g (NH4)2SO4 .................................................................................... 0.5g KH2PO4......................................................................................... 0.5g

Thermococcus litoralis Medium

Resazurin ................................................................................... 1.0mg (NH4)2Ni(SO4)2.......................................................................... 0.3mg Na2WO4·2H2O ......................................................................... 0.15mg Na2SeO4 ................................................................................... 0.15mg Synthetic seawater .................................................................485.0mL Trace elements solution SL-6 ..................................................15.0mL Na2S·9H2O solution .................................................................10.0mL NaHCO3 solution .....................................................................10.0mL pH 6.7 ± 0.2 at 25°C

Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.5g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature. NaHCO3 Solution: Composition per 10.0mL: NaHCO3 ........................................................................................ 0.2g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Filter sterilize. Trace Elements Solution SL-6: Composition per liter: MnCl2·4H2O.................................................................................. 0.5g H3BO3 ........................................................................................... 0.3g CoCl2·6H2O .................................................................................. 0.2g ZnSO4·7H2O ................................................................................ 0.1g Na2MoO4·2H2O ......................................................................... 0.03g NiCl2·6H2O ................................................................................. 0.02g CuCl2·2H2O ................................................................................ 0.01g

Preparation of Trace Elements Solution SL-6: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Synthetic Seawater: Composition per liter: NaCl ........................................................................................ 23.477g MgCl2·6H2O.............................................................................. 4.981g Na2SO4 ...................................................................................... 3.917g CaCl2 .......................................................................................... 1.12g KCl......................................................................................... 664.0mg NaHCO3 ................................................................................. 192.0mg H3BO3 ...................................................................................... 26.0mg SrCl2 ........................................................................................ 24.0mg KBr............................................................................................. 6.0mg NaF............................................................................................. 3.0mg

Preparation of Synthetic Seawater: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Preparation of Purified Chitin: Cool 200.0mL of 37% HCl to 4°C. Add 20.0g chitin (practical grade from crab shells) to the cooled HCl. Mix thoroughly. Stir for 60 min at 4°C. Pour the suspension into 1 liter of distilled water, pre-cooled to 4°C. Filter through filter paper. Wash the residue five times with 500.0mL distilled water. Resuspend in 1.0L of distilled water. Neutralize the suspension with 10.0mL of 5M KOH to achieve a final pH of 6.5. Filter and wash with 3.0L of distilled water to remove KCl. Allow to air dry. © 2010 by Taylor and Francis Group, LLC

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Preparation of Medium: Add components, except NaHCO3 solu-

tion and Na2S·9H2O solution, to distilled/deionized water and bring volume to 980.0mL. Gently heat and bring to boiling. Boil for 5 min. Cool to 25°C while sparging with 100% N2. Add 10.0mL NaHCO3 solution. Adjust pH to 6.7. Distribute the medium into Hungate tubes under an atmosphere of 100% N2. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to room temperature. Reduce the medium by adding 10.0mL Na2S·9H2O solution. Aseptically and anaerobically distribute to sterile tubes or bottles.

Use: For the cultivation of Thermococcus chitinophagus.

Thermococcus litoralis Medium Composition per liter: NaCl............................................................................................ 25.0g Sulfur .......................................................................................... 10.0g (NH4)2SO4 .................................................................................... 1.3g Yeast extract.................................................................................. 1.0g Peptone ......................................................................................... 0.5g KH2PO4....................................................................................... 0.28g MgSO4·7H2O .............................................................................. 0.25g CaCl2·2H2O ................................................................................ 0.07g FeCl2·2H2O................................................................................. 0.02g NaB4O·10H2O ........................................................................... 4.5mg MnCl2·4H2O .............................................................................. 1.8mg Resazurin ................................................................................... 1.0mg ZnSO4·7H2O ............................................................................ 0.22mg CuCl2·2H2O ............................................................................. 0.05mg NaMoO4·2H2O......................................................................... 0.03mg VOSO4·2H2O........................................................................... 0.03mg CoSO4 ...................................................................................... 0.01mg Na2S·9H2O solution .................................................................10.0mL pH 7.2 ± 0.2 at 25°C

Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O.................................................................................... 0.3g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Preparation of Medium: Add components, except Na2S·9H2O solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Do not autoclave. Sterilize by steaming at 100°C for 30 min on 3 consecutive days. Before inoculation, add 10.0mL of sterile Na2S·9H2O solution. Mix thoroughly. Use: For the cultivation of Thermococcus litoralis.

Thermococcus litoralis Medium Composition per liter: NaCl.......................................................................................... 19.45g MgCl2·6H2O ............................................................................... 12.6g Sulfur, powdered......................................................................... 10.0g Peptone ......................................................................................... 5.0g Na2SO4 ........................................................................................ 3.42g CaCl2·2H2O ................................................................................ 2.38g Yeast extract.................................................................................. 1.0g Na2CO3 ....................................................................................... 0.61g KCl.............................................................................................. 0.55g Resazurin ...................................................................................... 0.1g KBr ............................................................................................. 0.08g SrCl2·6H2O ............................................................................... 0.057g

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Thermococcus Medium

H3BO3 ....................................................................................... 0.022g Na2HPO4 ..................................................................................... 0.01g Na2SiO3·9H2O............................................................................ 4.0mg NaF............................................................................................. 2.4mg KNO3 ......................................................................................... 1.6mg Na2S·9H2O solution .................................................................10.0mL pH 6.5 ± 0.2 at 25°C

Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.5g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Prepare and dispense medium under 100% N2. Add components, except Na2S·9H2O solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Adjust pH to 6.5. Sparge with 100% N2. Do not autoclave. Sterilize by steaming for 3 hr at 100°C on 3 consecutive days. Prior to inoculation, aseptically and anaerobically add 10.0mL of sterile Na2S·9H2O solution.

Use: For the cultivation of Thermococcus litoralis.

Thermococcus Medium (DSMZ Medium 806) Composition per liter: NaCl ............................................................................................ 18.0g Sulfur ............................................................................................ 5.0g MgSO4·7H2O ................................................................................ 3.4g MgCl2·2H2O.................................................................................. 2.7g Yeast extract.................................................................................. 1.0g Trypticase™.................................................................................. 1.0g NaHCO3 ........................................................................................ 1.0g KCl.............................................................................................. 0.33g NH4Cl ......................................................................................... 0.25g CaCl2·2H2O................................................................................. 0.14g K2HPO4 ....................................................................................... 0.14g Resazurin .................................................................................. 0.001g Na2SeO3 ................................................................................. 0.001mg NiCl2·6H2O ............................................................................ 0.001mg Trace elements solution ...........................................................10.0mL Vitamin solution.......................................................................10.0mL Cysteine solution......................................................................10.0mL Na2S·9H2O solution .................................................................10.0mL pH 7.2 ± 0.2 at 25°C

Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.5g

Vitamin Solution: Composition per liter: Pyridoxine-HCl........................................................................ 10.0mg Thiamine-HCl·2H2O.................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg D-Ca-pantothenate ..................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize. Preparation of Medium: Add components, except Fildes enrichment solution, NaHCO3, vitamin solution, cysteine solution, and Na2S·9H2O solution, to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for 5 min. Cool to room temperature under 100% N2. Add 1.0g solid NaHCO3. Adjust pH to 7.2. Sterilize at 100°C for 3 hr on 3 consecutive days. Aseptically and anaerobically add 10.0mL vitamin solution, 10.0mL cysteine solution, and 10.0mL Na2S·9H2O solution. Mix thoroughly. Adjust pH to 7.2. Aseptically and anaerobically distribute to sterile tubes or bottles.

Use: For the cultivation of Thermococcus spp.

Thermococcus profundus Medium

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

Composition per 1010.0mL:

Cysteine Solution: Composition per 10.0mL:

NaCl............................................................................................ 25.0g Sulfur .......................................................................................... 10.0g Peptone ......................................................................................... 5.0g Yeast extract.................................................................................. 1.0g Resazurin ................................................................................... 1.0mg Salt base solution ..........................................................................1.0L Na2S·9H2O solution .................................................................10.0mL pH 7.2 ± 0.2 at 25°C

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature. Adjust pH to 7.0.

L-Cysteine·HCl·H2O ..................................................................... 0.3g

Preparation of Sulfur: Sterilize powdered elemental sulfur by steaming for 3 hr at 0 psi pressure–100°C on 3 successive days.

Thermodesulfobacterium Medium

Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.5g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Before use, neutralize to pH 7.0 with sterile HCl.

Salt Base Solution: Composition per liter: (NH4)2SO4 ..................................................................................... 1.3g KH2PO4 ....................................................................................... 0.28g MgSO4·7H2O .............................................................................. 0.25g CaCl2·2H2O................................................................................. 0.07g FeCl3·6H2O ................................................................................. 0.02g Na2B4O7·10H2O......................................................................... 4.5mg MnCl2·4H2O............................................................................... 1.8mg ZnSO4·7H2O ............................................................................ 0.22mg CuCl2·2H2O ............................................................................. 0.05mg Na2MoO4·2H2O ....................................................................... 0.03mg VOSO4 ..................................................................................... 0.03mg CoSO4·7H2O ............................................................................ 0.02mg

Preparation of Salt Base Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Preparation of Medium: Prepare and dispense medium under 100% N2. Add components, except Na2S·9H2O solution, to salt base solution and bring volume to 1.0L. Mix thoroughly. Adjust medium pH to 7.2 with H2SO4. Autoclave for 15 min at 15 psi pressure–121°C. Immediately prior to use, aseptically and anaerobically add 10.0mL of sterile Na2S·9H2O solution. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or bottles.

Use: For the cultivation of Thermococcus profundus.

Thermococcus stetteri Medium Composition per liter: NaCl ............................................................................................ 25.0g Sulfur .......................................................................................... 10.0g Yeast extract.................................................................................. 3.0g (NH4)2SO4 ..................................................................................... 1.3g Peptone.......................................................................................... 0.5g KH2PO4 ....................................................................................... 0.28g MgSO4·7H2O .............................................................................. 0.25g CaCl2·2H2O................................................................................. 0.07g FeCl2·2H2O ................................................................................. 0.02g NaB4O·10H2O............................................................................ 4.5mg MnCl2·4H2O............................................................................... 1.8mg Resazurin ................................................................................... 1.0mg ZnSO4·7H2O ............................................................................ 0.22mg CuCl2·2H2O ............................................................................. 0.05mg NaMoO4·2H2O......................................................................... 0.03mg VOSO4·2H2O ........................................................................... 0.03mg CoSO4 ...................................................................................... 0.01mg Na2S·9H2O solution .................................................................10.0mL pH 6.5 ± 0.2 at 25°C

1723

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Preparation of Medium: Add components, except Na2S·9H2O solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.5. Do not autoclave. Sterilize by steaming at 100°C for 30 min on 3 consecutive days. Before inoculation, add 10.0mL of sterile Na2S·9H2O solution. Mix thoroughly. Use: For the cultivation of Thermococcus stetteri.

Thermodesulfobacterium Medium Composition per liter: Na2SO4 .......................................................................................... 3.0g Na2HPO4·12H2O........................................................................... 2.0g NH4Cl ........................................................................................... 1.0g KH2PO4......................................................................................... 0.3g MgCl2·6H2O ................................................................................. 0.2g FeSO4·7H2O............................................................................... 1.5mg Resazurin ................................................................................... 1.0mg Sodium lactate solution............................................................20.0mL Trace elements solution ...........................................................10.0mL Yeast extract solution...............................................................10.0mL Na2S·9H2O solution .................................................................10.0mL Vitamin solution.........................................................................5.0mL pH 6.8 ± 0.2 at 25°C

Sodium Lactate Solution: Composition per 20.0mL: Sodium lactate .............................................................................. 4.0g

Preparation of Sodium Lactate Solution: Add sodium lactate to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure– 121°C.

Trace Elements Solution: Composition per liter: Nitrilotriacetic acid ..................................................................... 12.8g NaCl.............................................................................................. 1.0g FeCl·4H2O .................................................................................... 0.20 CoCl2·6H2O................................................................................ 0.17g CaCl2·2H2O .................................................................................. 0.1g MnCl2·4H2O ................................................................................. 0.1g ZnCl2 ............................................................................................ 0.1g CuCl2 .......................................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O.......................................................................... 0.01g NiCl2·6H2O............................................................................... 0.026g Na2SeO3·5H2O............................................................................ 0.02g

Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Yeast Extract Solution: Composition per 10.0mL: Yeast extract.................................................................................. 1.0g

Preparation of Yeast Extract Solution: Add yeast extract to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

1724

Thermodesulfobium Medium

Na2S·9H2O Solution: Composition per 10.0mL:

Trace Elements Solution SL-9: Composition per liter:

Na2S·9H2O .................................................................................... 0.5g

MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl.............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoSO4·7H2O ............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O ............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O ............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Prior to use, neutralize solution by dropwise addition of sterile 1N HCl.

Vitamin Solution: Composition per liter: Pyridoxine·HCl ........................................................................ 10.0mg Calcium DL-pantothenate........................................................... 5.0mg Lipoic acid ................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg p-Aminobenzoic acid ................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Thiamine·HCl ............................................................................ 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Prepare and dispense medium under 100% N2. Add components, except sodium lactate solution, yeast extract solution, and Na2S·9H2O solution, to distilled/deionized water and bring volume to 960.0mL. Mix thoroughly. Sparge with 100% N2. Anaerobically distribute into tubes or bottles. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add to 1.0L of medium 20.0mL of sterile sodium lactate solution, 10.0mL of sterile yeast extract solution, and 10.0mL of sterile Na2S·9H2O solution. Mix thoroughly.

Use: For the cultivation and maintenance of Thermodesulfobacterium commune.

Thermodesulfobium Medium (DSMZ Medium 1005) Composition per liter: Na2SO4 ......................................................................................... 2.8g KH2PO4 ....................................................................................... 0.78g K2HPO4 ....................................................................................... 0.75g NH4Cl .......................................................................................... 0.5g MgSO4·7H2O .............................................................................. 0.25g NaCl ............................................................................................. 0.2g Na3-EDTA.................................................................................. 0.04g CaCl2·2H2O................................................................................. 0.03g Na-acetate .................................................................................. 0.15g FeSO4·7H2O................................................................................ 0.01g Vitamin solution.......................................................................10.0mL Trace elements solution SL-9 ..................................................10.0mL Cysteine solution......................................................................10.0mL pH 5.7 ± 0.2 at 25°C

Cysteine Solution: Composition per 10.0mL: L-Cysteine-HCl·2H2O ................................................................. 0.25g

Preparation of Cysteine Solution: Add L-cysteine to to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize. © 2010 by Taylor and Francis Group, LLC

Preparation of Trace Elements Solution SL-9: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Mix thoroughly.

Vitamin Solution: Composition per liter: Pyridoxine-HCl........................................................................ 10.0mg Thiamine-HCl·2H2O.................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg D-Ca-pantothenate ..................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize. Preparation of Medium: Add components, except vitamin and cysteine solutions, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for 3 min. Cool to room temperature while sparging with a gas mixture of 80% N2 + 20% CO2. Adjust the pH to 5.5 with 10N H2SO4. Dispense under the same gas atmosphere in culture vessels (e.g., 20.0mL of the medium into 50mL serum bottles). Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aspetically add vitamin solution. Mix thoroughly. Prior to inoculation change atmosphere to 80% H2 and 20% CO2 gas mixture. Add the cysteine solution. Adjust pH to 5.5–6.0 if necessary. After inoculation pressurize vials to 0.5 bar overpressure with 80% H2 and 20% CO2 gas mixture. Use: For the cultivation of Thermodesulfobium narugense.

Thermodesulforhabdus Medium Composition per liter: NaCl............................................................................................ 10.0g Na2SO4 .......................................................................................... 7.0g Sodium acetate·3H2O.................................................................... 6.2g MgCl2·6H2O ................................................................................. 3.0g KH2PO4......................................................................................... 1.0g NH4Cl ......................................................................................... 0.25g CaCl2·2H2O ................................................................................ 0.15g Resazurin ................................................................................... 0.5mg

Thermodesulfotobacterium Agar

Na2S·9H2O solution .................................................................10.0mL Na2S2O4 solution......................................................................10.0mL Trace elements solution SL-10 ..................................................1.0mL NaHCO3 solution .................................................................... variable pH 6.8 ± 0.2 at 25°C

NaHCO3 Solution: Composition per 20.0mL: NaHCO3 ........................................................................................ 1.0g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/de-

ionized water and bring volume to 20.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure– 121°C.

Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................. 0.15g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

1725

Mineral solution 2....................................................................50.0mL Na2CO3 solution......................................................................50.0mL Mineral solution 1....................................................................25.0mL Cysteine-sulfide reducing agent ..............................................20.0mL Wolfe’s mineral solution..........................................................10.0mL Wolfe’s vitamin solution..........................................................10.0mL Resazurin (0.025% solution) .....................................................4.0mL pH 7.2 ± 0.2 at 25°C

Mineral Solution 1: Composition per liter: K2HPO4......................................................................................... 6.0g

Preparation of Mineral Solution 1: Add K2HPO4 to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Mineral Solution 2: Composition per liter:

Na2S2O3 Solution: Composition per 10.0mL:

NaCl............................................................................................ 12.0g KH2PO4......................................................................................... 6.0g (NH4)2SO4 .................................................................................... 6.0g MgSO4·7H2O ................................................................................ 2.4g CaCl2·2H2O .................................................................................. 1.6g

Na2S2O3·5H2O .............................................................................. 2.0g

Preparation of Mineral Solution 2: Add components to distilled/

Preparation of Na2S2O3 Solution: Add Na2S2O3·5H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

deionized water and bring volume to 1.0L. Mix thoroughly.

Trace Elements Solution SL-10: Composition per liter:

Na2CO3 ......................................................................................... 8.0g

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O........................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution) ..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly.

Preparation of Medium: Prepare and dispense medium under 100% N2. Add components, except Na2S·9H2O solution, Na2S2O3 solution, and NaHCO3 solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Cool to room temperature while sparging with 100% N2. Anaerobically distribute 9.8mL volumes into tubes. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add 0.1mL of sterile Na2S·9H2O solution and 0.1mL of sterile Na2S2O4 solution to each tube. Aseptically and anaerobically add a sufficient volume of sterile NaHCO3 solution to each tube to bring the pH to 6.8.

Use: For the cultivation of Thermodesulforhabdus norvegicus.

Thermodesulfotobacterium Agar Composition per liter: Na2SO4 ........................................................................................ 30.0g Agar ............................................................................................ 20.0g Sodium lactate............................................................................... 4.0g Yeast extract.................................................................................. 1.0g © 2010 by Taylor and Francis Group, LLC

Na2CO3 Solution:

Composition per 100.0mL:

Preparation of Na2CO3 Solution: Add Na2CO3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Cysteine-Sulfide Reducing Agent: Composition per 20.0mL: L-Cysteine·HCl·H2O .............................................................. 300.0mg Na2S·9H2O............................................................................. 300.0mg

Preparation of Cysteine-Sulfide Reducing Agent: Add

L-

cysteine·HCl·H2O to 10.0mL of distilled/deionized water. Mix thoroughly. In a separate tube, add Na2S·9H2O to 10.0mL of distilled/deionized water. Mix thoroughly. Gas both solutions with 100% N2 and cap tubes. Autoclave both solutions for 15 min at 15 psi pressure– 121°C using fast exhaust. Cool to 50°C. Aseptically combine the two solutions under 100% N2.

Wolfe’s Mineral Solution: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl.............................................................................................. 1.0g MnSO4·H2O .................................................................................. 0.5g FeSO4·7H2O.................................................................................. 0.1g CoCl2·6H2O .................................................................................. 0.1g CaCl2 ............................................................................................. 0.1g ZnSO4·7H2O ................................................................................. 0.1g CuSO4·5H2O............................................................................... 0.01g AlK(SO4)2·12H2O....................................................................... 0.01g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O .......................................................................... 0.01g

Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L.

1726

Thermodesulfotobacterium Broth

Wolfe’s Vitamin Solution: Composition per liter:

Cysteine-Sulfide Reducing Agent: Composition per 20.0mL:

Pyridoxine·HCl ........................................................................ 10.0mg Thiamine·HCl ............................................................................ 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg Calcium pantothenate ................................................................ 5.0mg p-Aminobenzoic acid ................................................................. 5.0mg Thioctic acid .............................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Cyanocobalamin .....................................................................100.0μg

L-Cysteine·HCl·H2O............................................................... 300.0mg Na2S·9H2O............................................................................. 300.0mg

Preparation of Wolfe’s Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except vitamin solution and cysteine-sulfide reducing agent, to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C under 80% N2 + 20% CO2. Aseptically add the sterile vitamin solution and then the sterile cysteine-sulfide reducing agent. Adjust the pH to 7.2. Distribute aseptically and anaerobically into sterile tubes. Use: For the cultivation and maintenance of Thermodesulfobacterium commune and other Thermodesulfobacterium species.

Preparation of Cysteine-Sulfide Reducing Agent: Add

L-

Wolfe’s Mineral Solution: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl.............................................................................................. 1.0g MnSO4·H2O .................................................................................. 0.5g FeSO4·7H2O.................................................................................. 0.1g CoCl2·6H2O .................................................................................. 0.1g CaCl2 ............................................................................................. 0.1g ZnSO4·7H2O ................................................................................. 0.1g CuSO4·5H2O ............................................................................... 0.01g AlK(SO4)2·12H2O....................................................................... 0.01g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O .......................................................................... 0.01g

Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic

Thermodesulfotobacterium Broth Composition per liter: Na2SO4 ........................................................................................ 30.0g Sodium lactate............................................................................... 4.0g Yeast extract.................................................................................. 1.0g Mineral solution 2....................................................................50.0mL Na2CO3 solution......................................................................50.0mL Mineral solution 1....................................................................25.0mL Cysteine-sulfide reducing agent...............................................20.0mL Wolfe’s mineral solution ..........................................................10.0mL Wolfe’s vitamin solution ..........................................................10.0mL Resazurin (0.025% solution)......................................................4.0mL pH 7.2 ± 0.2 at 25°C

acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L.

Wolfe’s Vitamin Solution: Composition per liter:

Mineral Solution 1: Composition per liter:

Pyridoxine·HCl ........................................................................ 10.0mg Thiamine·HCl ............................................................................ 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg Calcium pantothenate ................................................................ 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Thioctic acid .............................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Cyanocobalamin ..................................................................... 100.0μg

K2HPO4 ......................................................................................... 6.0g

Preparation of Wolfe’s Vitamin Solution: Add components to

Preparation of Mineral Solution 1: Add K2HPO4 to distilled/de-

distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Mineral Solution 2: Composition per liter:

Preparation of Medium: Add components, except vitamin solu-

ionized water and bring volume to 1.0L. Mix thoroughly.

NaCl ............................................................................................ 12.0g KH2PO4 ......................................................................................... 6.0g (NH4)2SO4 ..................................................................................... 6.0g MgSO4·7H2O ................................................................................ 2.4g CaCl2·2H2O................................................................................... 1.6g

Preparation of Mineral Solution 2: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly.

Na2CO3 Solution: Composition per 100.0mL:

tion and cysteine-sulfide reducing agent, to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool under 80% N2 + 20% CO2. Aseptically add the sterile vitamin solution and then the sterile cysteine-sulfide reducing agent. Adjust the pH to 7.2. Distribute aseptically and anaerobically into sterile tubes.

Use: For the cultivation and maintenance of Thermodesulfobacterium commune and other Thermodesulfobacterium species.

Thermodesulfovibrio yellowstonii Medium (DSMZ Medium 749)

Na2CO3 ......................................................................................... 8.0g

Composition per liter:

Preparation of Na2CO3 Solution: Add Na2CO3 to distilled/de-

Na2SO4 .......................................................................................... 4.0g Na-lactate...................................................................................... 2.5g

Thermofilum pendens Medium

NaHCO3 ........................................................................................ 1.3g KCl............................................................................................... 0.5g Yeast extract.................................................................................. 0.5g MgCl2·6H2O.................................................................................. 0.4g NH4Cl ......................................................................................... 0.25g Na2HPO4 ....................................................................................... 0.2g Na-thioglycolate............................................................................ 0.2g L-Ascorbic acid ............................................................................. 0.2g CaCl2·2H2O................................................................................. 0.15g Resazurin ................................................................................... 0.5mg Trace elements solution ...........................................................10.0mL Vitamin solution.......................................................................10.0mL pH 7.5 ± 0.2 at 25°C

Trace Elements Solution: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl .............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoSO4·7H2O ............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O................................................................................... 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O ............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O ............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize.

Preparation of Medium: Prepare and dispense medium under an oxygen-free 100% N2. Add components, except vitamin solution, Nathioglycolate, NaHCO3, and L-Ascorbic acid, to distilled/deionized water and bring volume to 990.0L. Mix thoroughly. Gently heat and bring to boiling. Cool while sparging with 100% N2. Add 0.2g Na-thioglycolate, 1.3g NaHCO3, and 0.2g L-ascorbic acid. Mix thoroughly. Adjust pH to 7.5. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically and anaerobically add 10.0mL sterile vitamin solution. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or flasks.

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Thermofilum pendens Medium Composition per liter: Sulfur, powdered......................................................................... 10.0g (NH4)2SO4 .................................................................................... 1.3g KH2PO4....................................................................................... 0.28g MgSO4·7H2O .............................................................................. 0.25g CaCl2·2H2O ................................................................................ 0.07g Na2S·9H2O.................................................................................... 0.3g FeCl3·6H2O................................................................................. 0.02g Na2B4O7·10H2O......................................................................... 4.5mg MnCl2·4H2O .............................................................................. 1.8mg ZnSO4·7H2O ............................................................................ 0.22mg CuCl2·2H2O ............................................................................. 0.05mg Na2MoO4·2H2O ....................................................................... 0.03mg VOSO4·2H2O........................................................................... 0.03mg CoSO4·7H2O............................................................................ 0.01mg Yeast extract solution...............................................................20.0mL Sucrose solution.......................................................................20.0mL Polar lipid fraction ............................................................6.0–12.0mL pH 5.2 ± 0.2 at 25°C

Yeast Extract Solution: Composition per 20.0mL: Yeast extract.................................................................................. 2.0g

Preparation of Yeast Extract Solution: Add yeast extract to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for a few minutes. Sparge with 100% N2. Do not autoclave. Sucrose Solution: Composition per 20.0mL: Sucrose.......................................................................................... 2.0g

Preparation of Sucrose Solution: Add sucrose to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize. Sparge with 100% N2. Preparation of Sulfur: Add 10.0g of powdered sulfur to a flask and sterilize by steaming for 3 hr on 3 consecutive days.

Polar Lipid Fraction: Composition per 20.0mL: Thermoproteus tenax cells (wet weight)..................................... 10.0g Chloroform ............................................................................500.0mL Acetone ..................................................................................500.0mL Methanol ................................................................................500.0mL TA buffer solution....................................................................80.0mL Chloroform/methanol 1:1 (v/v)................................................20.0mL

TA Buffer Solution: Composition per 100.0mL: Tris·HCl ...................................................................................... 0.79g β-mercaptoethanol ...................................................................... 0.78g NH4Cl ....................................................................................... 0.118g EDTA ........................................................................................ 0.029g

Preparation of TA Buffer Solution: Add components to distilled/ deionized water and bring volume to 100.0mL. Mix thoroughly.

Preparation of Polar Lipid Fraction: Add 10.0g (wet weight) of Thermoproteus tenax cells to 20.0mL of TA buffer solution. Mix thoroughly. Sonicate for 2 min. Centrifuge at 20,000 rpm for 20 min. Resuspend pellet in 20.0mL of fresh TA buffer solution. Recentrifuge at 20,000 rpm for 20 min. Again resuspend pellet in 20.0mL of fresh TA buffer solution. Recentrifuge at 20,000 rpm for 20 min. Resuspend pellet in 20.0mL of fresh TA buffer solution. Centrifuge at 5,000 rpm for

1728

Thermogymnomonas Medium

5 min. Decant the supernatant solution and discard the pellet. Extract the supernatant solution twice with 20.0mL of chloroform/methanol (1:1) each time. Chromatograph the extract on a SIL-LC (325 mesh) silicic acid column (20cm × 2cm) using 500.0mL of chloroform, followed by 500.0mL of acetone, followed by 500.0mL of methanol. The methanol fraction is further purified by DEAE chromatography using chloroform/methanol 1:1 and methanol.

H3BO3 ....................................................................................... 10.0μg MnSO4·5H2O ............................................................................ 10.0μg MoO3 ........................................................................................ 10.0μg CuSO4·5H2O ............................................................................... 5.0μg n-Heptadecane ...........................................................................1.0mL pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Prepare and dispense medium under

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 1.0mL of n-heptadecane. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

100% N2. Add components, except yeast extract solution, sucrose solution, sulfur, and polar lipid fraction, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Sparge with 100% N2. Anaerobically distribute into tubes or bottles. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add to 1.0L of medium 20.0mL of sterile yeast extract solution, 20.0mL of sterile sucrose solution, 10.0g of sterile sulfur, and 6.0–12.0mL of sterile polar lipid fraction. Mix thoroughly.

Use: For the cultivation and maintenance of Thermofilum pendens.

Thermogymnomonas Medium (DSMZ Medium 1141) Composition per liter: KH2PO4 ......................................................................................... 3.0g (NH4)2SO4 ..................................................................................... 0.2g MgSO4·7H2O ................................................................................ 0.5g CaCl2·2H2O.................................................................................. 025g Glucose solution ......................................................................50.0mL Yeast extract solution ...............................................................50.0mL pH 3.0 ± 0.2 at 25°C

Glucose Solution: Composition per 50.0mL: Glucose ....................................................................................... 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.

Yeast Extract Solution: Composition per 50.0mL: Yeast extract.................................................................................. 1.0g

Preparation of Yeast Extract Solution: Add yeast extract to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except glucose and yeast extract solutions, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to room temperature. Aspetically add glucose and yeast extract solutions. Mix thoroughly. Adjust pH to 3.0 with sterile 10N H2SO4.

Use: For the cultivation of Thermogymnomonas spp.

Thermoleophilum Medium Composition per liter: NaNO2........................................................................................... 2.0g Na2HPO4 ..................................................................................... 0.21g MgSO4·7H2O ................................................................................ 0.2g KCl.............................................................................................. 0.04g NaH2PO4 .................................................................................. 90.0mg CaCl2 ........................................................................................ 15.0mg FeSO4·7H2O............................................................................... 1.0mg ZnSO4·7H2O .............................................................................70.0μg © 2010 by Taylor and Francis Group, LLC

Preparation of Medium: Add components, except n-heptadecane,

Use: For the cultivation of Thermoleophilum album and Thermoleophilum minutum.

Thermomicrobium fosteri Agar Composition per liter: Agar ............................................................................................ 20.0g NH4Cl ........................................................................................... 2.0g Na2HPO4 ..................................................................................... 0.21g MgSO4·7H2O ................................................................................ 0.2g NaH2PO4 ..................................................................................... 0.09g KCl.............................................................................................. 0.04g CaCl2 ......................................................................................... 0.015g ZnSO4·7H2O ............................................................................. 70.0μg H3BO3 ....................................................................................... 10.0μg MnSO4·5H2O ............................................................................ 10.0μg MoO3 ........................................................................................ 10.0μg CuSO4·5H2O ............................................................................... 5.0μg FeSO4·7H2O............................................................................... 1.0mg Heptadecane, filter sterilized ...................................................20.0mL pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except heptadecane, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 20.0mL of sterile heptadecane. Mix thoroughly. Aseptically distribute into sterile tubes. Cool tubes rapidly in a slanted position.

Use: For the cultivation and maintenance of Thermomicrobium fosteri.

Thermomicrobium fosteri Broth Composition per liter: NH4Cl ........................................................................................... 2.0g Na2HPO4 ..................................................................................... 0.21g MgSO4·7H2O ................................................................................ 0.2g NaH2PO4 ..................................................................................... 0.09g KCl.............................................................................................. 0.04g CaCl2 ......................................................................................... 0.015g ZnSO4·7H2O ............................................................................. 70.0μg H3BO3 ....................................................................................... 10.0μg MnSO4·5H2O ............................................................................ 10.0μg MoO3 ........................................................................................ 10.0μg CuSO4·5H2O ............................................................................... 5.0μg FeSO4·7H2O............................................................................... 1.0mg Heptadecane, filter sterilized ...................................................20.0mL pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except heptadecane, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add

Thermophilic Hydrogen-Bacteria Medium

20.0mL of sterile heptadecane. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Thermomicrobium fosteri.

Thermomicrobium roseum Agar (DSMZ Medium 592) Composition per liter: Agar ............................................................................................ 20.0g (NH4)2SO4 (sublimed) .................................................................. 1.3g Yeast extract.................................................................................. 1.0g Tryptone ........................................................................................ 1.0g MgSO4·7H2O ............................................................................ 0.247g KH2PO4 ..................................................................................... 0.280g CaCl2·2H2O............................................................................... 0.074g FeCl3·6H2O ............................................................................... 0.019g Salt solution ...............................................................................1.0mL pH 8.5 ± 0.2 at 25°C

Salt Solution: Composition per liter: Na2B4O7·10H2O............................................................................ 4.4g MnCl2·4H2O.................................................................................. 1.8g ZnSO4·7H2O ............................................................................... 0.22g CuCl2·H2O .................................................................................. 0.05g Na2MoO4·4H2O .......................................................................... 0.03g VOSO4·2H2O .............................................................................. 0.03g

Preparation of Salt Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Adjust pH to 2.0. Mix thoroughly. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.5. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour into Petri dishes or pour short slants with a long butt in screw-capped tubes.

Use: For the cultivation and maintenance of Thermomicrobium roseum.

Thermomicrobium roseum Medium (DSMZ Medium 592) Composition per liter: (NH4)2SO4 (sublimed) .................................................................. 1.3g Yeast extract.................................................................................. 1.0g Tryptone ........................................................................................ 1.0g MgSO4·7H2O ............................................................................ 0.247g KH2PO4 ..................................................................................... 0.280g CaCl2·2H2O............................................................................... 0.074g FeCl3·6H2O ............................................................................... 0.019g Salt solution ...............................................................................1.0mL pH 8.5 ± 0.2 at 25°C

Salt Solution: Composition per liter: Na2B4O7·10H2O............................................................................ 4.4g MnCl2·4H2O.................................................................................. 1.8g ZnSO4·7H2O ............................................................................... 0.22g CuCl2·H2O .................................................................................. 0.05g Na2MoO4·4H2O .......................................................................... 0.03g VOSO4·2H2O .............................................................................. 0.03g

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Use: For the cultivation of Thermomicrobium roseum.

Thermomonospora Medium Composition per liter: Sucrose........................................................................................ 30.0g Agar ............................................................................................ 15.0g Casamino acids ............................................................................. 6.0g NaNO3 .......................................................................................... 3.0g Yeast extract.................................................................................. 2.0g K2HPO4......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g KCl................................................................................................ 0.5g FeSO4·7H2O................................................................................ 0.01g pH 8.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Thermomonospora alba and Thermomonospora mesophila.

Thermophilic Bacillus Medium Composition per liter: Peptone ......................................................................................... 8.0g Yeast extract.................................................................................. 4.0g NaCl.............................................................................................. 3.0g pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of a variety of thermophilic Bacillus species.

Thermophilic Hydrogen-Bacteria Medium Composition per 1000.5mL: Na2HPO4·12H2O........................................................................... 4.5g KH2PO4......................................................................................... 1.5g NaCl.............................................................................................. 1.0g NH4NO3 ........................................................................................ 1.0g MgSO4·7H2O ................................................................................ 0.2g CaCl2·2H2O ............................................................................. 10.0mg FeSO4·7H2O............................................................................. 10.0mg Trace elements solution .............................................................0.5mL pH 7.0 ± 0.2 at 25°C

Trace Elements Solution: Composition per liter: ZnSO4·7H2O ............................................................................ 28.0mg CoCl2·6H2O ............................................................................... 4.0mg H3BO3 ........................................................................................ 4.0mg MnSO4·5H2O ............................................................................. 4.0mg MoO3 ......................................................................................... 4.0mg CuSO4·5H2O.............................................................................. 2.0mg

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

1730

Thermophilic Maintenance Medium

CuSO4·5H2O ............................................................................... 0.01g AlK(SO4)2·12H2O....................................................................... 0.01g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O .......................................................................... 0.01g

Use: For the cultivation and maintenance of Hydrogenobacter ther-

Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic

mophilus and Pseudomonas species.

Thermophilic Maintenance Medium Composition per liter: NaHCO3 ........................................................................................ 3.0g Yeast extract.................................................................................. 1.0g NH4Cl ........................................................................................... 1.0g KH2PO4 ......................................................................................... 0.4g K2HPO4 ......................................................................................... 0.4g MgSO4·7H2O ................................................................................ 0.1g Cysteine-sulfide reducing solution ..........................................40.0mL Fructose solution......................................................................25.0mL Wolfe’s vitamin solution ..........................................................10.0mL Wolfe’s mineral solution ..........................................................10.0mL Resazurin (0.01% solution)........................................................1.0mL pH 5.6 ± 0.2 at 25°C

Cysteine-Sulfide Reducing Solution: Composition per 100.0mL: L-Cysteine·HCl·H2O

................................................................... 1.25g Na2S·9H2O .................................................................................. 1.25g

Preparation of Cysteine-Sulfide Reducing Solution: Add Lcysteine·HCl·H2O and Na2S·9H2O to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Fructose Solution: Composition per 100.0mL: Fructose....................................................................................... 20.0g

Preparation of Fructose Solution: Add fructose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Wolfe’s Vitamin Solution: Composition per liter: Pyridoxine·HCl ........................................................................... 0.01g Thiamine·HCl ............................................................................ 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg Calcium pantothenate ................................................................ 5.0mg p-Aminobenzoic acid ................................................................. 5.0mg Thioctic acid .............................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Cyanocobalamin .....................................................................100.0μg

Preparation of Wolfe’s Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L.

Preparation of Medium: Add components, except cysteine-sulfide reducing solution and fructose solution, to distilled/deionized water and bring volume to 935.0mL. Mix thoroughly. Gently heat and bring to boiling. Continue boiling until resazurin turns colorless, indicating reduction. Add 40.0mL of the cysteine-sulfide reducing solution. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C under O2free 90% N2 + 10% CO2. Add 25.0mL of the sterile fructose solution. Adjust the pH to 5.6 if necessary. Aseptically and anaerobically distribute into sterile tubes. Cap with rubber stoppers. Use: For the cultivation and maintenance of a variety of thermophilic anaerobes, including Clostridium thermoautotrophicum.

Thermophilic Methanosarcina Medium Composition per 1021.0mL: NaCl............................................................................................ 2.25g Pancreatic digest of casein............................................................ 2.0g Yeast extract.................................................................................. 2.0g NaHCO3...................................................................................... 0.85g MgSO4·7H2O................................................................................ 0.5g NH4Cl ........................................................................................... 0.5g K2HPO4 .................................................................................... 0.348g CaCl2·2H2O ................................................................................ 0.25g KH2PO4 .................................................................................... 0.227g FeSO4·7H2O .............................................................................. 2.0mg Resazurin ................................................................................... 1.0mg Rumen fluid, clarified..............................................................50.0mL Methanol solution ....................................................................10.0mL Vitamin solution.......................................................................10.0mL L-Cysteine·HCl·H2O solution ..................................................10.0mL Na2S·9H2O solution .................................................................10.0mL Trace elements solution SL-10 ..................................................1.0mL pH 6.5–6.8 at 25°C

Methanol Solution: Composition per 10.0mL: Methanol ....................................................................................5.0mL

Preparation of Methanol Solution: Add methanol to distilled/deionized water and bring volume to 10.0mL. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Vitamin Solution: Composition per liter: Pyridoxine·HCl ........................................................................ 10.0mg Calcium DL-pantothenate........................................................... 5.0mg Lipoic acid ................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Thiamine·HCl ............................................................................ 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Thermophilic Methanothrix Medium

1731

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Sparge with 80% N2 + 20% CO2.

NaHCO3 Solution: Composition per 20.0mL:

L-Cysteine·HCl·H2O

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/de-

Solution: Composition per 10.0mL:

L-Cysteine·HCl·H2O ..................................................................... 0.3g

Preparation of L-Cysteine·HCl·H2O Solution: Add L-cyste-

ine·HCl·H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C.

Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.3g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Sparge with 100% N2.

Preparation of Medium: Add components, except methanol solution, L-cysteine·HCl·H2O solution, and Na2S·9H2O solution, to distilled/deionized water and bring volume to 960.0mL. Mix thoroughly. Sparge under 80% N2 + 20% CO2 for 3–4 min. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add 20.0mL of sterile vitamin solution, 10.0mL of sterile L-cysteine·HCl·H2O solution, and 10.0mL of sterile Na2S·9H2O solution. Mix thoroughly. Aseptically and anaerobically distribute into sterile screw-capped bottles under 80% N2 + 20% CO2.

Use: For the cultivation and maintenance of Methanosarcina thermophila.

Thermophilic Methanothrix Medium Composition per liter: NH4Cl ........................................................................................... 0.5g K2HPO4 ......................................................................................... 0.4g MgCl2·6H2O.................................................................................. 0.1g Resazurin ................................................................................... 1.0mg NaHCO3 solution .....................................................................20.0mL Trace elements solution ..........................................................10.0mL CaCl2·2H2O solution................................................................10.0mL Sodium acetate solution ...........................................................10.0mL Vitamin solution.......................................................................10.0mL Coenzyme M solution ..............................................................10.0mL Na2S·9H2O solution ...................................................................5.0mL pH 6.5 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

NaHCO3 ........................................................................................ 1.0g ionized water and bring volume to 20.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2 for 15 min. Autoclave for 15 min at 15 psi pressure–121°C.

Trace Elements Solution: Composition per liter: MgSO4·7H2O ............................................................................... 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl.............................................................................................. 1.0g MnSO4·2H2O ............................................................................... 0.5g CoSO4·7H2O............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g CuSO4·5H2O............................................................................... 0.01g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O ......................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic acid to approximately 500.0mL of distilled/deionized water. Dissolve by adding KOH and adjust pH to 6.5. Add remaining components. Bring volume to 1.0L with additional distilled/deionized water. Adjust pH to 7.0 with KOH. CaCl2·2H2O Solution: Composition per 10.0mL: CaCl2·2H2O .................................................................................. 0.1g

Preparation of CaCl2·2H2O Solution: Add CaCl2·2H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Sodium Acetate Solution: Composition per 10.0mL: Sodium acetate.............................................................................. 3.3g

Preparation of Sodium Acetate Solution: Add sodium acetate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure– 121°C. Vitamin Solution: Composition per liter: Pyridoxine·HCl ........................................................................ 10.0mg Calcium DL-pantothenate........................................................... 5.0mg Lipoic acid ................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Thiamine·HCl ............................................................................ 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% N2 + 20% CO2.

1732

Thermophilic Spirochete Medium

Coenzyme M Solution: Composition per 10.0mL: Coenzyme M............................................................................. 0.142g

Preparation of Coenzyme M Solution: Add coenzyme M to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.5g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except NaHCO3 solu-

tion, CaCl2·2H2O solution, sodium acetate solution, vitamin solution, coenzyme M solution, and Na2S·9H2O solution, to distilled/deionized water and bring volume to 935.0mL. Gently heat and bring to boiling. Continue boiling for 10 min. Cool to room temperature while sparging with 80% N2 + 20% CO2. Gas the medium until the pH reaches 5.8. Anaerobically distribute the medium into serum bottles. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add 20.0mL of sterile NaHCO3 solution, 10.0mL of sterile CaCl2·2H2O solution, 10.0mL of sterile sodium acetate solution, 10.0mL of sterile vitamin solution, 10.0mL of sterile coenzyme M solution, and 5.0mL of sterile Na2S·9H2O solution. Bring gas atmosphere in each bottle to 30% CO2.

Use: For the cultivation and maintenance of Methanothrix thermophila.

Thermophilic Spirochete Medium Composition per 1012.0mL: Solution A ..............................................................................920.0mL Solution D ................................................................................50.0mL Solution E (Vitamin solution) ..................................................20.0mL Solution F.................................................................................10.0mL Solution G ................................................................................10.0mL Solution B (Trace elements solution SL-10)..............................1.0mL Solution C (Selenite-tungstate solution) ....................................1.0mL pH 6.9 ± 0.2 at 25°C

Solution A: Composition per 920.0mL: NaCl .............................................................................................. 4.0g MgCl2·6H2O.................................................................................. 0.8g KCl................................................................................................ 0.5g NH4Cl ........................................................................................... 0.3g KH2PO4 ......................................................................................... 0.2g CaCl2·2H2O................................................................................. 0.03g Resazurin ................................................................................... 1.0mg

Preparation of Solution A: Prepare and dispense under 80% N2 + 20% CO2. Add components to distilled/deionized water and bring volume to 920.0mL. Mix thoroughly. Gently heat and bring to boiling. Continue boiling for a few minutes. Cool to room temperature while sparging with 80% N2 + 20% CO2. Anaerobically distribute into tubes or bottles. Autoclave for 15 min at 15 psi pressure–121°C. Solution B (Trace Elements Solution SL-10): Composition per liter: FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg © 2010 by Taylor and Francis Group, LLC

MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Solution C (Selenite-Tungstate Solution): Composition per liter: NaOH............................................................................................ 0.5g Na2SeO3·5H2O........................................................................... 3.0mg Na2WO4·2H2O ........................................................................... 4.0mg

Preparation of Solution C (Selenite-Tungstate Solution): Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Solution D: Composition per 50.0mL: NaHCO3 ..................................................................................... 3.0mg

Preparation of Solution D: Add NaHCO3 to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Solution E (Vitamin Solution): Composition per liter: Pyridoxine·HCl ........................................................................ 10.0mg Calcium DL-pantothenate........................................................... 5.0mg Lipoic acid ................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Thiamine·HCl ............................................................................ 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Solution E (Vitamin Solution): Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Solution F: Composition per 10.0mL: Starch, soluble............................................................................... 1.0g

Preparation of Solution F: Add starch to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Solution G: Composition per 10.0mL: Na2S·9H2O.................................................................................... 0.3g

Preparation of Solution G: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Prepare and dispense medium under 80% N2 + 20% CO2. To 920.0mL of sterile solution A, aseptically and anaerobically add 1.0mL of sterile solution B, 1.0mL of sterile

Thermoplasma acidophilum Medium

solution C, 50.0mL of sterile solution D, 20.0mL of sterile solution E, 10.0mL of sterile solution F, and 10.0mL of sterile solution G in that order. Mix thoroughly.

Use: For the cultivation and maintenance of Spirochaeta thermophila.

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MnSO4 ........................................................................................ 0.05g CoSO4 ......................................................................................... 0.01g H3BO3 ......................................................................................... 0.01g CuSO4 ........................................................................................ 8.0mg

Preparation of Trace Elements Solution: Add components to

Thermophilic Streptomycete Medium Composition per liter: Agar ............................................................................................ 20.0g Maltose........................................................................................ 20.0g Soybean meal ................................................................................ 5.0g Yeast extract.................................................................................. 2.0g pH 6.5 ± 0.2 at 25°C

distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Use: For the isolation and cultivation of thermophilic streptomycetes.

Thermophilic Streptomycete Medium Composition per liter: Soybean oil meal......................................................................... 20.0g Glucose ....................................................................................... 10.0g NaCl ............................................................................................ 10.0g Pancreatic digest of casein .......................................................... 10.0g Silica solution (Ludox) ..........................................................500.0mL

Preparation of Medium: Add components, except silica solution, to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Gently heat until dissolved. Autoclave this solution and the 500.0mL of silica solution separately for 15 min at 15 psi pressure– 121°C. Cool to 25°C. Adjust the pH of both solutions to 7.0. Aseptically combine the two sterile solutions. Mix thoroughly. Pour into sterile Petri dishes in 40.0mL volumes.

Use: For the isolation and cultivation of thermophilic streptomycetes.

Thermophilic Streptomycete Medium IA Composition per liter: Agar ............................................................................................ 20.0g Sucrose.......................................................................................... 5.0g Pancreatic digest of casein ............................................................ 5.0g Yeast extract.................................................................................. 3.0g MgSO4·7H2O ................................................................................ 0.5g FeSO4·7H2O................................................................................ 0.01g Dung extract...............................................................................5.0mL Molasses.....................................................................................5.0mL Trace elements solution .............................................................1.0mL pH 7.2 ± 0.2 at 25°C

Dung Extract: Composition per 100.0mL: Sheep manure, dried ................................................................... 25.0g

Preparation of Dung Extract: Add dried sheep manure to 100.0mL of tap water. Mix thoroughly. Autoclave for 30 min at 15 psi pressure–121°C. Filter through Whatman #1 filter paper. Store at 4°C under toluene.

Trace Elements Solution: Composition per 100.0mL: Fe(NH4)2SO4 ................................................................................. 0.1g ZnSO4 ........................................................................................... 0.1g © 2010 by Taylor and Francis Group, LLC

Thermoplasma acidophilum Growth Medium 7B Composition per liter: Sucrose........................................................................................ 17.0g (NH4)2SO4 ................................................................................... .6.8g KOH............................................................................................ 1.22g Yeast extract.................................................................................. 1.0g MgSO4 .......................................................................................... 0.5g CaCl2·2H2O ................................................................................ 0.25g H3PO4 solution...........................................................................1.5mL Antifoam A ...............................................................................10.0μL pH 1.60 ± 0.2 at 25°C

H3PO4 Solution: Composition per 100.0mL: H3PO4 ......................................................................................... 85.0g

Preparation of H3PO4 Solution: Add H3PO4 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Preparation of Medium: Add components, except Antifoam A, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 1.60 with 50% H2SO4. Distribute into tubes or flasks. Sterilize by heating to 100°C for 30 min. Allow to stand at room temperature for 24 hr. Add 10.0μL of Antifoam A per liter. Use: For the cultivation of Thermococcus acidophilum.

Thermoplasma acidophilum Medium Composition per liter: (NH4)2SO4 .................................................................................. 1.32g Yeast extract solution.................................................................... 1.0g KH2PO4..................................................................................... 0.372g MgSO4·7H2O ............................................................................ 0.247g CaCl2·2H2O .............................................................................. 0.074g Glucose solution ......................................................................20.0mL Yeast extract solution...............................................................10.0mL Trace elements solution ...........................................................10.0mL pH 1.0–2.0 at 25°C

Glucose Solution: Composition per 20.0mL: Glucose ....................................................................................... 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Yeast Extract Solution: Composition per 10.0mL: Yeast extract.................................................................................. 1.0g

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Thermoplasma acidophilum Medium 7A

Preparation of Yeast Extract Solution: Add yeast extract to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Trace Elements Solution: Composition per liter: FeCl3·6H2O ................................................................................. 1.93g Na2B4O7·10H2O.......................................................................... 0.45g MnCl2·4H2O................................................................................ 0.18g ZnSO4·7H2O ............................................................................ 22.0mg CuCl2·2H2O ............................................................................... 5.0mg VOSO4·5H2O ............................................................................. 3.8mg Na2MoO4·2H2O ........................................................................ 3.0mg CoSO4·7H2O .............................................................................. 2.0mg

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, except glucose solution and yeast extract solution, to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Adjust pH to 1.0–2.0 with 10N H2SO4. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 20.0mL of sterile glucose solution and 10.0mL of sterile yeast extract solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation and maintenance of Thermoplasma acidophilum.

Thermoplasma acidophilum Medium 7A Composition per liter: Glucose ....................................................................................... 10.0g (NH4)2SO4 ..................................................................................... 6.8g KH2PO4 ......................................................................................... 3.0g Yeast extract.................................................................................. 1.0g MgSO4 .......................................................................................... 0.5g CaCl2·2H2O................................................................................. 0.25g pH 1.65 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 1.65 with 50% H2SO4. Distribute into tubes or flasks. Sterilize by heating to 100°C for 30 min.

Use: For the cultivation of Thermococcus acidophilum.

Thermoplasma Agar Composition per liter: Basal solution.........................................................................450.0mL Solution B ..............................................................................450.0mL Solution C ..............................................................................100.0mL pH 2.0 ± 0.2 at 25°C

Basal Solution: Composition per 500.0mL: KH2PO4 ......................................................................................... 3.0g Yeast extract.................................................................................. 1.0g MgSO4·7H2O ................................................................................ 0.5g CaCl2·2H2O................................................................................. 0.25g (NH4)2SO4 ..................................................................................... 0.2g

Preparation of Basal Solution: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 2.0 with 10N H2SO4. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 55°C. © 2010 by Taylor and Francis Group, LLC

Solution B: Composition per 450.0mL: Noble agar................................................................................... 12.0g

Preparation of Solution B: Add agar to distilled/deionized water and bring volume to 450.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 55°C.

Solution C: Composition per 100.0mL: Glucose ....................................................................................... 10.0g

Preparation of Solution C: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Aseptically combine the cooled, sterile basal medium with sterile solution B and sterile solution C. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation and maintenance of Thermoplasma acidophilum and other Thermoplasma species.

Thermoplasma Broth Composition per liter: Basal solution.........................................................................500.0mL Solution C ..............................................................................100.0mL pH 2.0 ± 0.2 at 25°C

Basal Solution: Composition per 500.0mL: KH2PO4......................................................................................... 3.0g Yeast extract.................................................................................. 1.0g MgSO4·7H2O ................................................................................ 0.5g CaCl2·2H2O ................................................................................ 0.25g (NH4)2SO4 .................................................................................... 0.2g

Preparation of Basal Solution: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 2.0 with 10N H2SO4. Solution C: Composition per 100.0mL: Glucose ....................................................................................... 10.0g

Preparation of Solution C: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add 500.0mL of basal solution to 400.0mL of distilled/deionized water. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 55°C. Aseptically add 100.0mL of sterile glucose solution. Mix thoroughly. Aseptically distribute into sterile tubes. Use: For the cultivation and maintenance of Thermoplasma acidophilum and other Thermoplasma species.

Thermoplasma volcanium Medium Composition per liter: KH2PO4......................................................................................... 3.0g MgSO4·7H2O ................................................................................ 1.0g CaCl2·2H2O ................................................................................ 0.25g (NH4)2SO4 .................................................................................... 0.2g Glucose solution ......................................................................10.0mL Yeast extract solution...............................................................10.0mL pH 6.5 ± 0.2 at 25°C

Thermosipho africanus Medium

Glucose Solution: Composition per 10.0mL: Glucose ......................................................................................... 5.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

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Preparation of Solution B: Add sulfur to 450.0mL of distilled/deionized water. Autoclave for 30 min at 0 psi pressure–100°C on 3 consecutive days.

Solution C: Composition per 50.0mL: Na2S·9H2O.................................................................................. 0.85g

Yeast extract.................................................................................. 1.0g

Preparation of Solution C: Add Na2S·9H2O to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Yeast Extract Solution: Add yeast extract to dis-

Preparation of Medium: Aseptically combine solutions A, B, and

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

C under 97% N2 + 3% H2. Adjust pH to 4.8–5.6 with H2SO4. Aseptically and anaerobically distribute into sterile tubes or flasks under 97% N2 + 3% H2.

Yeast Extract Solution: Composition per 10.0mL:

Preparation of Medium: Add components, except glucose solution and yeast extract solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Adjust pH to 2.0 with 10N H2SO4. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0mL of sterile glucose solution and 10.0mL of sterile yeast extract solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Final pH should be 2.0–3.0. Use: For the aerobic cultivation and maintenance of Thermoplasma volcanium.

Thermoproteus Medium Composition per liter: Solution A ..............................................................................500.0mL Solution B ..............................................................................450.0mL Solution C ................................................................................50.0mL pH 4.8–5.6 at 25°C

Solution A: Composition per 500.0mL: Glucose ....................................................................................... 10.0g FeSO4·7H2O.............................................................................. 0.556g MgSO4·7H2O ............................................................................ 0.492g CaSO4·2H2O ............................................................................. 0.344g (NH4)2SO4 ................................................................................. 0.264g Yeast extract.................................................................................. 0.2g KH2PO4 ..................................................................................... 0.014g Resazurin ................................................................................... 1.0mg Trace elements .........................................................................10.0mL

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Immediately filter sterilize. Trace Elements: Composition per liter: Na2B4O7·10H2O.......................................................................... 0.45g MnCl2·4H2O................................................................................ 0.18g ZnSO4·7H2O ............................................................................. 0.022g CuCl2·2H2O ............................................................................... 5.0mg Na2MoO4·2H2O ......................................................................... 3.6mg VOSO4·5H2O ............................................................................. 3.6mg CoSO4·7H2O .............................................................................. 1.2mg

Preparation of Trace Elements: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3.0 with H2SO4 to retard precipitation.

Use: For the cultivation and maintenance of Thermoproteus tenax and other Thermoproteus species.

Thermoproteus neutrophilus Medium Composition per liter: Sulfur, powdered........................................................................... 8.0g (NH4)2SO4 .................................................................................... 1.3g NaHCO3 ...................................................................................... 0.85g KH2PO4....................................................................................... 0.28g MgSO4·7H2O .............................................................................. 0.25g CaCl2·2H2O ................................................................................ 0.07g FeCl3·6H2O................................................................................. 0.02g Na2B4·10H2O ............................................................................. 4.5mg MnCl2·4H2O .............................................................................. 1.8mg Resazurin ................................................................................... 0.4mg ZnSO4·7H2O ............................................................................ 0.22mg CuCl2·2H2O ............................................................................. 0.05mg Na2MoO4·2H2O ....................................................................... 0.03mg VOSO4·2H2O........................................................................... 0.03mg CoSO4 ...................................................................................... 0.01mg Dithionite solution .....................................................................1.0mL pH 6.5 ± 0.2 at 25°C

Dithionite Solution: Composition per 10.0mL: Na2S2O4 ...................................................................................... 0.25g

Preparation of Dithionite Solution: Add Na2S2O4 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except sulfur, NaHCO3, resazurin, and dithionite solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Add sulfur and resazurin. Adjust pH to 6.5 with NaOH. Gently heat and bring to boiling. Continue boiling for 5 min. Cool to room temperature while sparging with 80% H2 + 20% CO2. Add NaHCO3. Mix thoroughly. Continue to sparge with 80% H2 + 20% CO2 until pH reaches 6.5. Distribute anaerobically 10.0mL of medium into 30.0mL serum bottles. Sterilize medium by heating at 85°C for 1 hr on 3 consecutive days. Prior to inoculation, add 10.0μL of sterile dithionite solution to each bottle.

Use: For the aerobic cultivation and maintenance of Thermoproteus neutrophilus.

Thermosipho africanus Medium Composition per liter: NaHCO3 ........................................................................................ 4.0g Sodium acetate.............................................................................. 4.0g

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Thermosphaera Medium

Sodium formate............................................................................. 2.0g Yeast extract.................................................................................. 1.0g L-Cysteine·HCl.............................................................................. 0.5g KH2PO4 ......................................................................................... 0.5g Na2S·9H2O .................................................................................... 0.5g MgSO4·7H2O ............................................................................... 0.4g NaCl .............................................................................................. 0.4g NH4Cl ........................................................................................... 0.4g CaCl2·2H2O................................................................................. 0.05g NiCl2·6H2O .............................................................................. 24.0mg FeSO4·7H2O.............................................................................. 2.0mg Resazurin ................................................................................... 1.0mg Sludge fluid..............................................................................50.0mL Fatty acid mixture ....................................................................20.0mL Trace elements solution SL-10 ..................................................1.0mL pH 6.7 ± 0.2 at 25°C

Sludge Fluid: Composition per 100.0mL: Sludge ....................................................................................100.0mL Yeast extract.................................................................................. 0.4g

Preparation of Sludge Fluid: To 100.0mL of sludge from an anaerobic digester, add 0.4g of yeast extract. Sparge with 100% N2 for a few minutes. Incubate at 37°C for 24 hr. Centrifuge the sludge at 13,000 × g for 15 min. Decant the clear supernatant solution. Sparge with 100% N2 for a few minutes. Store in screw-capped bottles at room temperature in the dark.

Fatty Acid Mixture: Composition per 20.0mL: α-Methylbutyric acid .................................................................... 0.5g Isobutyric acid............................................................................... 0.5g Isovaleric acid ............................................................................... 0.5g Valeric acid ................................................................................... 0.5g

Preparation of Fatty Acid Mixture: Add components to distilled/ deionized water and bring volume to 20.0mL. Mix thoroughly. Adjust pH to 7.5 with concentrated NaOH.

Thermosphaera Medium (DSMZ Medium 817) Composition per liter: MgCl2·6H2O ................................................................................. 2.2g Yeast extract.................................................................................. 1.0g Peptone ......................................................................................... 1.0g NaCl.............................................................................................. 0.9g KCl........................................................................................... 17.0mg NH4Cl ...................................................................................... 12.5mg CaCl2·2H2O ............................................................................... 7.0mg K2HPO4·3H2O ........................................................................... 7.0mg Resazurin ................................................................................... 0.4mg FeCl3 ........................................................................................ 0.05mg Vitamin solution.......................................................................10.0mL Na2S·9H2O solution .................................................................10.0mL NaHCO3 solution .....................................................................10.0mL pH 6.5 ± 0.2 at 25°C

NaHCO3 Solution: Composition per 10.0mL: NaHCO3 ........................................................................................ 1.0g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Must be prepared freshly. Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O.................................................................................... 0.5g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Sparge with N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Store anaerobically. Vitamin Solution: Composition per liter: Pyridoxine-HCl........................................................................ 10.0mg Thiamine-HCl·2H2O.................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg D-Ca-pantothenate ..................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize.

cally under 80% N2 + 20% CO2. Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Prepare and dispense medium under 80% N2 + 20% CO2. Add components, except Na2S·9H2O solution, vitamin solution, and NaHCO3 solution, to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Flush medium with 80% N2 + 20% CO2. for 5 min. Adjust medium pH to 6.53. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically and anaerobically add 10.0mL sterile Na2S·9H2O solution, 10.0mL sterile vitamin solution, and 10.0mL sterile NaHCO3 solution. Mix thoroughly. Aseptically and anaerobically distribute into sterile thick-walled tubes or thickwalled bottles. Pressurize with 2 bar atmosphere of 80% N2 + 20% CO2.

Use: For the cultivation and maintenance of Thermosipho africanus.

Use: For the cultivation of Thermosphaera aggregans.

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

Preparation of Medium: Prepare and dispense medium anaerobi-

Thermosyntropha Medium

Thermosulfidibacter Medium (DSMZ Medium 1092) Composition per liter: Sea salts, Sigma .......................................................................... 30.0g Sulfur, elemental ........................................................................... 3.0g Resazurin ................................................................................... 0.5mg Vitamin solution.......................................................................20.0mL Sulfide solution ........................................................................10.0mL Yeast extract solution ...............................................................10.0mL Bicarbonate solution ................................................................10.0mL Wolfe's mineral elixir.................................................................1.0mL pH 7.1 ± 0.2 at 25°C

Sulfide Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.5g

Preparation of Sulfide Solution: Add Na2S·9H2O to distilled/de-

ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Wolfe’s Mineral Elixir: Composition per liter: MgSO4·7H2O .............................................................................. 30.0g NaCl ............................................................................................ 10.0g MnSO4·2H2O ................................................................................ 5.0g (NH4)2NiSO4·6H2O ...................................................................... 2.8g CoCl2·6H2O .................................................................................. 1.8g ZnSO4·7H2O ................................................................................. 1.8g FeSO4·7H2O.................................................................................. 1.0g CaCl2·2H2O................................................................................... 1.0g KAl(SO4)2·12H2O....................................................................... 0.18g CuSO4·5H2O ................................................................................. 0.1g H3BO3 ........................................................................................... 0.1g Na2MoO4·2H2O ............................................................................ 0.1g Na2SeO4 ........................................................................................ 0.1g Na2WO4·2H2O .............................................................................. 0.1g

Preparation of Wolfe’s Mineral Elixir: Adjust pH of 1.0L of distilled/deionized water to 1.0 with dilute H2SO4. Add remaining components one at a time. Mix throughly to dissolve.

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize.

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Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with gas mixture of 80% N2 + 20% CO2. Filter sterilize.

Yeast Extract Solution: Composition per 10.0mL: Yeast extract ................................................................................. 2.0g

Preparation of Yeast Extract Solution: Add yeast extract to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2 gas. Autoclave for 15 min at 15 psi pressure– 121°C. Preparation of Medium: Add components, except vitamin, bicarbonate, sulfide, and yeast extract solutions, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for 1 min. Cool to room temperature while sparging with a gas mixture of 80% H2 + 20% CO2. Adjust the pH to 5.5 with 10N H2SO4. Dispense under the same gas atmosphere in culture vessels (balch tubes). Autoclave for 20 min at 6 psi pressure–110°C. Cool to room temperature. Aspetically add vitamin, bicarbonate, sulfide, and yeast extract solutions. Mix thoroughly. Prior to inoculation change atmosphere to 80% H2 and 20% CO2 gas mixture. Adjust pH to 7.0–7.2. After inoculation pressurize vials to 2.0 bar overpressure with 80% H2 and 20% CO2 gas mixture.

Use: For the cultivation of Thermosulfidibacter spp.

Thermosyntropha Medium (DSMZ Medium 731) Composition per liter: Yeast extract................................................................................ 10.0g Na2CO3 ......................................................................................... 3.0g NH4Cl ........................................................................................... 1.0g NaCl.............................................................................................. 0.5g K2HPO4......................................................................................... 0.3g KCl............................................................................................... 0.3g MgCl2·6H2O ................................................................................. 0.3g CaCl2·2H2O ................................................................................ 0.05g Resazurin ................................................................................... 0.5mg Vitamin solution.......................................................................40.0mL Trace elements solution ...........................................................10.0mL Cysteine solution .....................................................................10.0mL Na2S·9H2O solution .................................................................10.0mL NaHCO3 solution .....................................................................10.0mL Selenite-tungstate solution.........................................................1.0mL pH 8.5 ± 0.2 at 25°C

Selenite-Tungstate Solution Composition per liter: NaOH............................................................................................ 0.5g Na2WO4·2H2O ........................................................................... 4.0mg Na2SeO3·5H2O........................................................................... 3.0mg

Preparation of Selenite-Tungstate Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

NaHCO3 Solution: Composition per 10.0mL: NaHCO3 ........................................................................................ 3.0g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Must be prepared freshly.

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Thermoterrabacterium Medium

Cysteine Solution: Composition per 10.0mL: L-Cysteine-HCl·H2O ................................................................... 0.15g

Preparation of Cysteine Solution: Add L-cysteine·HCl·H2O to

distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure– 121°C.

Trace Elements Solution: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl .............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoSO4·7H2O ............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O................................................................................... 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O ............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O ............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Vitamin Solution: Composition per liter: Pyridoxine-HCl ........................................................................ 10.0mg Thiamine-HCl·2H2O .................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg D-Ca-pantothenate...................................................................... 5.0mg p-Aminobenzoic acid ................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize. Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.5g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

tilled/deionized water and bring volume to 10.0mL. Sparge with N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Store anaerobically.

Preparation of Medium: Prepare and dispense medium under 100% N2. Add components, except NaHCO3 solution, Na2S·9H2O solution, cysteine solution, vitamin solution, selenite-tungstate solution, and trace elements solution, to distilled/deionized water and bring volume to 919.0mL. Mix thoroughly. Sparge with 100% N2. Adjust pH to 8.5 with HCl. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add 10.0mL NaHCO3 solution, 10.0mL Na2S·9H2O solution, 10.0mL cysteine solution, 40.0mL vitamin solution, 1.0mL © 2010 by Taylor and Francis Group, LLC

selenite-tungstate solution, and 10.0mL trace elements solution. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or bottles.

Use: For the cultivation of Thermosyntropha lipolytica.

Thermoterrabacterium Medium (DSMZ Medium 778) Composition per liter: NaHCO3 ...................................................................................... 10.0g Na2-9,10-anthraquinone-2,6-disulfonate .................................... 8.25g Yeast extract.................................................................................. 1.0g KH2PO4....................................................................................... 0.33g NH4Cl ......................................................................................... 0.33g KCl............................................................................................. 0.33g MgCl2·6H2O ............................................................................... 0.33g NiCl2·6H2O ............................................................................. 200.0µg Na2SeO3·5H2O........................................................................ 120.0µg Na2WO4·2H2O .......................................................................... 30.0µg Calcium chloride solution........................................................10.0mL Vitamin solution.......................................................................10.0mL Glycerol (87%) ..........................................................................3.0mL Trace elements solution SL-10 ..................................................1.0mL pH 6.8 ± 0.2 at 25°C

Calcium Chloride Solution: Composition per 10.0mL: CaCl2·2H2O ................................................................................ 0.33g

Preparation of Calcium Chloride Solution: Add CaCl2·2H2O to

10.0mL of distilled/deionized water. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Trace Elements Solution SL-10: Composition per liter: FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

Vitamin Solution: Composition per liter: Pyridoxine-HCl........................................................................ 10.0mg Thiamine-HCl·2H2O.................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg D-Ca-pantothenate ..................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Thermotoga elfii Medium Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize. Preparation of Medium: Add components, except NaHCO3, calci-

um chloride solution, and vitamin solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for several minutes to dissolve the antraquinone. Cool to room temperature under an atmosphere of 100% CO2. Add solid NaHCO3. Adjust pH to 6.8 wtih NaOH. Dispense medium under CO2 into tubes or bottles. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically and anaerobically distribute into sterile tubes or bottles. Before use, add calcium chloride and vitamin solutions from anaerobic, sterile stock solution.

Use: For the cultivation of Thermoterrabacterium ferrireducens.

Thermotoga elfii Medium Composition per liter: NaCl ............................................................................................ 10.0g Pancreatic digest of casein ............................................................ 2.0g Yeast extract.................................................................................. 2.0g NH4Cl ........................................................................................... 1.0g K2HPO4 ......................................................................................... 0.3g KH2PO4 ......................................................................................... 0.3g MgCl2·6H2O.................................................................................. 0.2g CaCl2·2H2O................................................................................... 0.1g KCl................................................................................................ 0.1g L-Cysteine·HCl·H2O ..................................................................... 0.5g Sodium acetate .............................................................................. 0.5g Resazurin ................................................................................... 1.0mg Na2CO3 solution ......................................................................20.0mL Na2S·9H2O solution .................................................................20.0mL Modified Wolfe’s mineral solution ..........................................10.0mL pH 8.0 ± 0.2 at 25°C

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H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O .......................................................................... 0.01g Na2SeO3 ...................................................................................... 0.01g NaWO4·2H2O ............................................................................. 0.01g NiC12·6H2O ................................................................................ 0.01g

Preparation of Modified Wolfe’s Mineral Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add remaining components one at a time. Add distilled/deionized water to 1.0L. Adjust pH to 6.8. Preparation of Medium: Prepare and dispense medium under 100% N2. Add components, except Na2CO3 solution and Na2S·9H2O solution, to distilled/deionized water and bring volume to 960.0mL. Mix thoroughly. Adjust pH to 8.0 with 10M KOH. Gently heat and bring to boiling. Cool to room temperature while sparging with 100% N2. Anaerobically dispense into tubes in 5.0mL aliquots under an atmosphere of 80% N2 + 20% CO2. Autoclave for 45 min at 6 psi pressure–110°C. Just prior to use, aseptically and anaerobically add 0.1mL of sterile Na2CO3 solution and 0.1mL of sterile Na2S·9H2O solution to each tube.

Use: For the cultivation of Thermotoga elfii.

Thermotoga elfii Medium Composition per liter:

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure– 121°C.

NaCl............................................................................................ 10.0g Sodium thiosulfate·5H2O.............................................................. 5.0g Pancreatic digest of casein............................................................ 5.0g Yeast extract.................................................................................. 5.0g NH4Cl ........................................................................................... 1.0g K2HPO4......................................................................................... 0.3g KH2PO4......................................................................................... 0.3g MgCl2·6H2O ................................................................................. 0.2g CaCl2·2H2O .................................................................................. 0.1g KCl................................................................................................ 0.1g Sodium acetate.............................................................................. 0.5g Resazurin ................................................................................... 0.5mg Glucose solution ......................................................................20.0mL L-Cysteine solution ..................................................................10.0mL Na2CO3 solution ......................................................................10.0mL Na2S·9H2O solution .................................................................10.0mL Wolfe’s mineral solution..........................................................10.0mL

Na2S·9H2O Solution: Composition per 20.0mL:

Glucose Solution: Composition per 20.0mL:

NaHCO3 Solution: Composition per 20.0mL: NaHCO3 ........................................................................................ 2.0g

Na2S·9H2O .................................................................................... 0.4g

Glucose ......................................................................................... 4.0g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

tilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Before use, neutralize to pH 7.0 with sterile HCl.

Modified Wolfe’s Mineral Solution: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl .............................................................................................. 1.0g MnSO4·H2O .................................................................................. 0.5g CaCl2 ............................................................................................. 0.1g CoCl2·6H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g ZnSO4·7H2O ................................................................................. 0.1g AlK(SO4)2·12H2O....................................................................... 0.01g CuSO4·5H2O ............................................................................... 0.01g © 2010 by Taylor and Francis Group, LLC

L-Cysteine

Solution: Composition per 10.0mL:

L-Cysteine·HCl·H2O ..................................................................... 0.5g

Preparation of L-Cysteine Solution: Add L-cysteine·HCl·H2O to

distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure– 121°C.

Na2CO3 Solution: Composition per 10.0mL: Na2CO3 ......................................................................................... 2.0g

Preparation of Na2CO3 Solution: Add Na2CO3 to distilled/de-

ionized water and bring volume to 10.0mL. Mix thoroughly. Sparge

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Thermotoga hypogea Medium

with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure– 121°C.

Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.5g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

Na2S·9H2O solution .................................................................14.0mL Trace elements solution ...........................................................10.0mL pH 7.3 ± 0.2 at 25°C

Xylose Solution: Composition per 20.0mL: Xylose ........................................................................................... 3.0g

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Before use, neutralize to pH 7.0 with sterile HCl.

Preparation of Xylose Solution: Add xylose to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Wolfe’s Mineral Solution: Composition per liter:

NaHCO3 Solution: Composition per 20.0mL:

MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl .............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoCl2·6H2O .................................................................................. 0.1g ZnSO4·7H2O ................................................................................. 0.1g CaCl2·2H2O................................................................................... 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O ............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g CuSO4·5H2O ............................................................................... 0.01g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O .......................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Adjust pH to 6.8. Preparation of Medium: Prepare and dispense medium under 80% N2 + 20% CO2 gas atmosphere. Add components, except glucose solution, Na2CO3 solution, L-cysteine solution, and Na2S·9H2O solution, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Adjust pH to 6.8–7.0. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add 20.0mL of sterile glucose solution, 10.0mL of sterile Na2CO3 solution, 10.0mL of sterlie L-cysteine solution, and 10.0mL of sterile Na2S·9H2O solution. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or bottles.

Use: For the cultivation of Thermotoga elfii.

Thermotoga hypogea Medium (DSMZ Medium 794) Composition per liter: NaCl ............................................................................................ 10.0g Yeast extract.................................................................................. 2.0g Trypticase™.................................................................................. 2.0g NH4Cl ........................................................................................... 1.0g Na-acetate ..................................................................................... 0.5g L-Cysteine ..................................................................................... 0.5g K2HPO4 ......................................................................................... 0.3g KH2PO4 ......................................................................................... 0.3g MgCl2·6H2O.................................................................................. 0.2g CaCl2·2H2O................................................................................... 0.1g KCl................................................................................................ 0.1g Resazurin ................................................................................... 0.5mg Na-thiosulfate solution.............................................................20.0mL NaHCO3 solution .....................................................................20.0mL Xylose solution ........................................................................20.0mL © 2010 by Taylor and Francis Group, LLC

NaHCO3 ........................................................................................ 2.0g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Must be prepared freshly. Na-thiosulfate Solution: Composition per 20.0mL: Na2S2O3·5H2O .............................................................................. 5.0g

Preparation of Na-thiosulfate Solution: Add Na2S2O3·5H2O to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize. Na2S·9H2O Solution: Composition per 20.0mL: Na2S·9H2O.................................................................................... 0.6g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to distilled/deionized water and bring volume to 20.0mL. Sparge with N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Store anaerobically. Trace Elements Solution: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl.............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoSO4·7H2O ............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O ............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O ............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Medium: Prepare and dispense medium under 80% N2 + 20% CO2 gas mixture. Add components, except xylose solution, Na2S·9H2O solution, Na-thiosulfate solution, and NaHCO3 solution, to 926.0mL distilled/deionized water. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Adjust pH to 7.2–7.4. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C while sparging with 80% N2 + 20% CO2. Aseptically and anaerobically add 20.0mL sterile xylose solution, 14.0mL Na2S·9H2O solution, 20.0mL NaHCO3 solution, and 20.0mL

Thermotoga petrophila Medium

Na-thiosulfate solution, . Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or flasks.

Use: For the cultivation of Thermotoga hypogea.

Thermotoga Medium Composition per 1017.0mL: NaCl ............................................................................................ 20.0g Starch, soluble............................................................................... 5.0g NiCl2·6H2O ................................................................................... 2.0g KH2PO4 ......................................................................................... 0.5g Na2S·9H2O .................................................................................... 0.5g Yeast extract.................................................................................. 0.5g Resazurin ................................................................................... 1.0mg Artificial seawater..................................................................250.0mL Trace elements solution ...........................................................15.0mL pH 6.5 ± 0.2 at 25°C

Artificial Seawater: Composition per liter: NaCl ............................................................................................ 27.7g MgSO4·7H2O ................................................................................ 7.0g MgCl2·6H2O.................................................................................. 5.5g CaCl2·2H2O................................................................................. 2.25g KCl.............................................................................................. 0.65g NaBr.............................................................................................. 0.1g H3BO3 ...................................................................................... 30.0mg SrCl2·6H2O............................................................................... 15.0mg Citric acid................................................................................. 10.0mg KI ............................................................................................. 0.05mg

Preparation of Artificial Seawater: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly.

Trace Elements Solution: Composition per liter: MgSO4·7H2O ............................................................................... 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl .............................................................................................. 1.0g MnSO4·2H2O ............................................................................... 0.5g CoSO4·7H2O ............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O................................................................................... 0.1g FeSO4·7H2O.................................................................................. 0.1g KAI(SO4)2·12H2O....................................................................... 0.02g CuSO4·5H2O ............................................................................... 0.01g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O ......................................................................... 0.01g NiCl2·6H2O ............................................................................... 0.025g Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L.

Preparation of Medium: Prepare and dispense medium under 100% N2. Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

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Thermotoga 2 Medium Composition per 1015.0mL: Starch, soluble............................................................................... 5.0g NaCl............................................................................................ 3.46g MgSO4·7H2O .............................................................................. 0.88g EDTA·Na2 ................................................................................. 0.768g MgCl2·6H2O ............................................................................... 0.69g KH2PO4......................................................................................... 0.5g Na2S·9H2O.................................................................................... 0.5g Yeast extract.................................................................................. 0.5g CaCl2·2H2O ................................................................................ 0.14g KCl.............................................................................................. 0.08g NaBr......................................................................................... 12.5mg H3BO3 ...................................................................................... 3.75mg (NH4)2Ni(SO4)2 ......................................................................... 3.0mg SrCl2·6H2O ................................................................................ 1.9mg Resazurin ................................................................................... 1.0mg KI ........................................................................................... 0.006mg Trace elements solution ...........................................................15.0mL pH 7.0 ± 0.2 at 25°C

Trace Elements Solution: Composition per liter: MgSO4·7H2O ............................................................................... 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl.............................................................................................. 1.0g MnSO4·2H2O ............................................................................... 0.5g CoSO4·7H2O............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g KAI(SO4)2·12H2O ...................................................................... 0.02g CuSO4·5H2O............................................................................... 0.01g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O ......................................................................... 0.01g NiCl2·6H2O............................................................................... 0.025g Na2SeO3·5H2O........................................................................... 0.3mg

Use: For the cultivation and maintenance of Thermotoga thermarum.

Thermotoga petrophila Medium (DSMZ Medium 913) Composition per liter: MOPS ........................................................................................... 5.0g Yeast extract.................................................................................. 2.0g (NH4)2SO4 .................................................................................... 1.0g KH2PO4......................................................................................... 0.5g Resazurin ................................................................................... 0.5mg Artificial seawater..................................................................750.0mL Trace elements solution ...........................................................10.0mL Vitamin solution.......................................................................10.0mL Na2S·9H2O solution .................................................................10.0mL pH 7.0 ± 0.2 at 25°C

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Thermotoga subterranea Medium

Artificial Seawater: Composition per liter: NaCl ............................................................................................ 27.7g MgSO4·7H2O ................................................................................ 7.0g MgCl2·6H2O.................................................................................. 5.5g KCl.............................................................................................. 0.65g NaBr.............................................................................................. 0.1g H3BO3 ...................................................................................... 30.0mg SrCl2·6H2O............................................................................... 15.0mg Citric acid................................................................................. 10.0mg KI ............................................................................................ 0.05mg CaCl2·2H2O................................................................................ 2.25 g

Preparation of Artificial Seawater: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Trace Elements Solution: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl .............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoSO4·7H2O ............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O................................................................................... 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O ............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O ............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Mix thoroughly. Vitamin Solution: Composition per liter: Pyridoxine-HCl ........................................................................ 10.0mg Thiamine-HCl·2H2O .................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg D-Ca-pantothenate...................................................................... 5.0mg p-Aminobenzoic acid ................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

to 980.0mL with distilled/deionized water. Mix thoroughly. Gently heat and bring to boiling. Boil for 3 min. Cool to room temperature while sparging with 100% N2. Distribute under 100% N2 into anaerobe tubes or bottles. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add, per liter of medium, 10.0mL vitamin solution and 10.0mL Na2S·9H2O solution. Mix thoroughly. The final pH of the medium should be 7.0.

Use: For the cultivation of Thermotoga petrophila and Thermotoga naphthophila.

Thermotoga subterranea Medium Composition per liter: NaCl............................................................................................ 12.0g MgSO4·7H2O ................................................................................ 0.5g PIPES............................................................................................ 3.4g KCl................................................................................................ 2.0g Peptone ......................................................................................... 1.0g Na2S·9H2O.................................................................................... 0.5g Yeast extract.................................................................................. 0.5g NH4Cl ........................................................................................... 0.1g CaCl2·2H2O .............................................................................. 0.025g K2HPO4....................................................................................... 0.02g Resazurin ................................................................................... 0.5mg Wolfe’s mineral solution..........................................................10.0mL Wolfe’s vitamin solution..........................................................10.0mL pH 7.0 ± 0.2 at 25°C

Wolfe’s Vitamin Solution: Composition per liter: Pyridoxine·HCl ........................................................................ 10.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Thiamine·HCl ............................................................................ 5.0mg Calcium DL-pantothenate........................................................... 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Wolfe’s Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Wolfe’s Mineral Solution: Composition per liter:

tilled/deionized water and bring volume to 10.0mL. Sparge with N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Store anaerobically.

MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl.............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoCl2·6H2O .................................................................................. 0.1g ZnSO4·7H2O ................................................................................. 0.1g CaCl2·2H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O ............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g CuSO4·5H2O ............................................................................... 0.01g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O .......................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Medium: Add components, except vitamin solution

Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic

and Na2S·9H2O solution, to 750.0mL artificial seawater. Bring volume

acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

Thermovibrio Medium

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KOH. Add remaining components. Add distilled/deionized water to 1.0L. Adjust pH to 6.8.

Vitamin Solution: Composition per liter:

Preparation of Medium: Prepare and dispense medium under

100% N2. Add components, except Wolfe’s vitamin solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Adjust pH to 7.0. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add 10.0mL of sterile Wolfe’s vitamin solution. Mix thoroughly. Adjust medium pH to 7.5 by adding sterile anaerobic 1N NaOH. Aseptically and anaerobically distribute into sterile tubes or bottles.

Use: For the cultivation of Thermotoga subterranea.

Thermovenabulum Medium (DSMZ Medium 962) Composition per liter: NaHCO3 ........................................................................................ 0.7g NH4Cl ......................................................................................... 0.33g KH2PO4 ....................................................................................... 0.33g MgCl2·6H2O................................................................................ 0.33g CaCl2·2H2O................................................................................. 0.33g KCl.............................................................................................. 0.33g Yeast extract................................................................................ 0.05g Meat extract solution ...............................................................50.0mL Sodium fumarate solution ........................................................50.0mL Vitamin solution.......................................................................10.0mL Trace elements solution ...........................................................10.0mL Selenite-tungstate solution.........................................................1.0mL pH 7.0 ± 0.2 at 25°C

Preparation of Selenite-Tungstate Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize. Trace Elements Solution: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl .............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoSO4·7H2O ............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O................................................................................... 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O ............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O ............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize. Sodium Fumarate Solution: Composition per 50.0mL: Na2-fumarate............................................................................... 3.20g

Preparation of Sodium Fumarate Solution: Add Na2-fumarate to distilled/deionized water and bring volume to 50.0mL. Sparge with N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Meat Extract Solution: Composition per 50.0mL: Meat extract .................................................................................. 3.0g

Preparation of Meat Extract Solution: Add meat extract to distilled/deionized water and bring volume to 50.0mL. Sparge with N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Preparation of Medium: Add components, except NaHCO3, vitamin solution, sodium fumarate solution, and meat extract solution, to distilled/deionized water and bring volume to 890.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for 3 min. Cool to 25°C while sparging with 80% N2 + 20% CO2. Add solid NaHCO3. Adjust pH to 6.8–7.0. Distribute to anaerobe tubes or bottles under 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add, per liter of medium, 10.0mL sterile vitamin solution, 50.0mL sterile meat extract solution, and 50.0mL sterile sodium fumarate solution. Mix thoroughly. The final pH should be 6.5. Use: For the cultivation of Thermovenabulum ferriorganovorum.

Thermovibrio Medium (DSMZ Medium 961) Composition per liter: Synthetic seawater .................................................................970.0mL Potassium nitrate solution........................................................10.0mL Yeast extract solution...............................................................10.0mL Na2S·9H2O solution .................................................................10.0mL pH 6.5 ± 0.2 at 25°C

Synthetic Seawater: Composition per liter: NaCl............................................................................................ 27.7g MgSO4·7H2O ................................................................................ 7.0g MgCl2·6H2O ................................................................................. 5.5g CaCl2·2H2O ................................................................................ 0.75g KCl.............................................................................................. 0.65g KH2PO4......................................................................................... 0.5g (NH4)2SO4 .................................................................................. 0.25g NaBr.............................................................................................. 0.1g H3BO3 ......................................................................................... 0.03g

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Thermus Agar

SrCl2·6H2O............................................................................... 15.0mg Resazurin ................................................................................... 1.0mg KI ............................................................................................ 0.05mg

Na2MoO4·2H2O....................................................................... 25.0mg Concentrated H2SO4 ..................................................................0.5mL

Preparation of Synthetic Seawater: Add components to distilled

tilled/deionized water and bring volume to 1.0L. Mix thoroughly.

water and bring volume to 1.0L. Mix thoroughly.

Fe-Citrate Solution: Composition per liter:

Na2S·9H2O Solution: Composition per 10.0mL:

Preparation of Micronutrient Solution: Add components to dis-

Fe-citrate .................................................................................. 24.5mg

Na2S·9H2O .................................................................................... 0.5g

Preparation of Fe-Citrate Solution: Add Fe-citrate to distilled/

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

deionized water and bring volume to 1.0L. Mix thoroughly.

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Yeast Extract Solution: Composition per 10.0mL: Yeast extract.................................................................................. 1.0g

Potassium Nitrate Solution: Composition per 10.0mL: KNO3 .......................................................................................... 0.33g

Preparation of Potassium Nitrate Solution: Add KNO3 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Preparation of Medium: Gently heat and bring 1.0L synthetic seawater to boiling. Boil for 3 min. Cool to 25°C while sparging with 80% H2 + 20% CO2. Adjust pH to 6.8–7.0. Distribute 20.0mL aliquots into 100mL serum bottles under 80% H2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add per 20.0mL of medium, 0.2mL sterile potassium nitrate solution, 0.2mL sterile yeast extract solution, and 0.2mL sterile Na2S·9H2O solution. Mix thoroughly. Adjusted pH should be 6.5.

Use: For the cultivation of Thermovibrio ruber.

Thermus Agar Composition per liter: Agar ............................................................................................ 28.0g Pancreatic digest of casein ............................................................ 2.5g Yeast extract.................................................................................. 2.5g Na2HPO4·12H2O ........................................................................ 0.43g MgCl2·6H2O ................................................................................. 0.2g Nitrilotriacetic acid ....................................................................... 0.1g KH2PO4 ................................................................................... 54.0mg CaSO4·2H2O ............................................................................ 40.0mg Micronutrient solution ...............................................................1.0mL Fe-citrate solution ......................................................................0.5mL pH 7.2 ± 0.2 at 25°C

Micronutrient Solution: Composition per liter: MnSO4·H2O................................................................................ 2.28g H3BO3 ........................................................................................... 0.5g ZnSO4·7H2O ................................................................................. 0.5g CoCl2·6H2O ............................................................................. 45.0mg CuSO4·5H2O............................................................................ 25.0mg © 2010 by Taylor and Francis Group, LLC

Preparation of Medium: Add nitrilotriacetic acid to 100.0mL of distilled/deionized water. Mix thoroughly. Adjust pH to 6.5 with KOH. Add remaining components and bring volume to 1.0L with distilled/deionized water. Mix thoroughly. Adjust pH to 7.2. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Thermus species.

Thermus BP Medium (Thermus Beef Extract Polypeptone™ Medium) Composition per liter: Agar ............................................................................................ 25.0g Beef extract................................................................................... 4.0g Polypeptone™ .............................................................................. 4.0g K2HPO4......................................................................................... 3.0g KH2PO4......................................................................................... 1.0g pH 7.0 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Thermus aquaticus and other Thermus species.

Thermus brockii Medium Composition per liter: Pancreatic digest of casein............................................................ 1.0g Yeast extract.................................................................................. 1.0g Salts solution..........................................................................100.0mL pH 7.6 ± 0.2 at 25°C

Salts Solution: Composition per liter: NaNO3 ........................................................................................ 6.89g KNO3 .......................................................................................... 1.03g MgSO4·7H2O ................................................................................ 1.0g Nitrilotriacetic acid ....................................................................... 1.0g CaSO4·2H2O ................................................................................. 0.6g NaCl......................................................................................... 80.0mg FeCl3 solution...........................................................................10.0mL Trace elements solution ...........................................................10.0mL

Preparation of Salts Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Adjust pH to 8.2 with 1M NaOH. Add remaining components. Add distilled/deionized water to 1.0L.

FeCl3 Solution: Composition per 100.0mL: FeCl3 ........................................................................................ 28.0mg

Preparation of FeCl3 Solution: Add FeCl3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Thermus Enhanced Agar with 1% Sodium Chloride

Trace Elements Solution: Composition per liter: MnSO4·H2O .................................................................................. 2.2g H3BO3 ........................................................................................... 0.5g ZnSO4·7H2O ................................................................................. 0.5g CoCl2·6H2O ............................................................................. 46.0mg Na2MoO4·2H2O ....................................................................... 25.0mg CuSO4 ...................................................................................... 16.0mg H2SO4 .........................................................................................0.5mL

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Thermus brockii.

1745

Nitrilotriacetic acid ....................................................................... 0.1g CaSO4·2H2O ............................................................................ 40.0mg Phosphate buffer solution ......................................................100.0mL Ferric citrate solution.................................................................0.5mL Trace elements solution .............................................................0.5mL

Phosphate Buffer Solution: Composition per liter: Na2HPO4·12H2O......................................................................... 43.0g KH2PO4....................................................................................... 5.44g

Preparation of Phosphate Buffer Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Ferric Citrate Solution: Composition per 10.0mL: Ferric citrate............................................................................. 24.5mg

Thermus Broth Composition per liter: Pancreatic digest of casein ............................................................ 2.5g Yeast extract.................................................................................. 2.5g Na2HPO4·12H2O ........................................................................ 0.43g MgCl2·6H2O ................................................................................. 0.2g Nitrilotriacetic acid ....................................................................... 0.1g KH2PO4 ................................................................................... 54.0mg CaSO4·2H2O ............................................................................ 40.0mg Micronutrient solution ...............................................................1.0mL Fe-citrate solution ......................................................................0.5mL pH 7.2 ± 0.2 at 25°C

Preparation of Micronutrient Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Fe-Citrate Solution: Composition per liter: Fe-citrate .................................................................................. 24.5mg

Preparation of Fe-Citrate Solution: Add Fe-citrate to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add nitrilotriacetic acid to 100.0mL of distilled/deionized water. Mix thoroughly. Adjust pH to 6.5 with KOH. Add remaining components and bring volume to 1.0L with distilled/ deionized water. Mix thoroughly. Adjust pH to 7.2. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Thermus species.

Thermus Enhanced Agar Composition per liter: Agar ............................................................................................ 28.0g Pancreatic digest of casein ............................................................ 2.5g Yeast extract.................................................................................. 2.5g MgCl2·6H2O.................................................................................. 0.2g © 2010 by Taylor and Francis Group, LLC

Preparation of Ferric Citrate Solution: Add ferric citrate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Trace Elements Solution: Composition per liter: Nitrilotriacetic acid ..................................................................... 12.8g FeCl2·4H2O................................................................................... 1.0g MnCl2·4H2O ................................................................................. 0.5g CoCl2·6H2O .................................................................................. 0.3g CuCl2·2H2O ............................................................................. 50.0mg Na2MoO4·2H2O ....................................................................... 50.0mg H3BO3 ...................................................................................... 20.0mg NiCl2·6H2O.............................................................................. 20.0mg

Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Preparation of Medium: Add components, except phosphate buffer solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.2 with NaOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°– 55°C. Aseptically add 100.0mL of sterile phosphate buffer solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Thermus species.

Thermus Enhanced Agar with 1% Sodium Chloride Composition per liter: Agar ............................................................................................ 28.0g NaCl............................................................................................ 10.0g Pancreatic digest of casein............................................................ 2.5g Yeast extract.................................................................................. 2.5g MgCl2·6H2O ................................................................................. 0.2g Nitrilotriacetic acid ....................................................................... 0.1g CaSO4·2H2O ............................................................................ 40.0mg Phosphate buffer solution ......................................................100.0mL Ferric citrate solution.................................................................0.5mL Trace elements solution .............................................................0.5mL

1746

Thermus Medium

Ferric Citrate Solution: Composition per 10.0mL: Ferric citrate ............................................................................. 24.5mg

Preparation of Ferric Citrate Solution: Add ferric citrate to dis-

Mineral Solution 1: Composition per liter: NaNO3 .......................................................................................... 6.9g KNO3 ............................................................................................ 1.8g Na2HPO4 ..................................................................................... 1.11g MgSO4·7H2O ................................................................................ 1.0g Nitrilotriacetic acid ....................................................................... 1.0g CaSO4·2H2O ................................................................................. 0.6g NaCl............................................................................................ 0.08g

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Preparation of Mineral Solution 1: Add nitrilotriacetic acid to

Trace Elements Solution: Composition per liter:

500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to and bring volume to 1.0L. Mix thoroughly.

Nitrilotriacetic acid ..................................................................... 12.8g FeCl2·4H2O ................................................................................... 1.0g MnCl2·4H2O.................................................................................. 0.5g CoCl2·6H2O .................................................................................. 0.3g CuCl2·2H2O ............................................................................. 50.0mg Na2MoO4·2H2O ....................................................................... 50.0mg H3BO3 ...................................................................................... 20.0mg NiCl2·6H2O .............................................................................. 20.0mg

Mineral Solution 2: Composition per liter: MnSO4·2H2O .............................................................................. 0.22g ZnSO4·7H2O ............................................................................... 0.05g H3BO3 ......................................................................................... 0.05g CuSO4·5H2O .............................................................................. 2.5mg

Preparation of Mineral Solution 2: Add components to distilled/

Preparation of Trace Elements Solution: Add nitrilotriacetic

deionized water to 1.0L. Mix thoroughly.

acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L.

Ferric Chloride Solution: Composition per 100.0mL:

Preparation of Medium: Add components, except phosphate buf-

Preparation of Ferric Chloride Solution: Add FeCl3·6H2O to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

fer solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.2 with NaOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°– 55°C. Aseptically add 100.0mL of sterile phosphate buffer solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Thermus species.

Thermus Medium Composition per liter: Agar ............................................................................................ 30.0g Polypeptone™............................................................................... 8.0g Yeast extract.................................................................................. 4.0g NaCl .............................................................................................. 2.0g pH 7.5 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Thermus aquaticus and other Thermus species.

Thermus Medium (DSMZ Medium 1033) Composition per liter: Tryptone ........................................................................................ 1.0g Yeast extract.................................................................................. 1.0g Mineral solution 1 .................................................................100.0mL Mineral solution 2 ...................................................................10.0mL Ferric chloride solution ............................................................10.0mL pH 8.2 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

FeCl3·6H2O ................................................................................ 4.6mg

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.2 with 1M NaOH. Dispense into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Thermus spp.

Thermus 162 Medium (DSMZ Medium 878) Composition per liter: Agar ............................................................................................ 28.0g Yeast extract.................................................................................. 1.0g Tryptone........................................................................................ 1.0g MgCl2·6H2O .......................................................................... 200.0mg Nitrilotriacetic acid ................................................................ 100.0mg CaSO4·2H2O ............................................................................ 40.0mg Phosphate buffer ....................................................................100.0mL Ferric citrate solution.................................................................0.5mL Trace elements solution .............................................................0.5mL pH 7.2 ± 0.2 at 25°C

Ferric Citrate Solution: Composition per 10.0mL: Ferric citrate............................................................................. 24.5mg

Preparation of Ferric Citrate Solution: Add ferric citrate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Trace Elements Solution: Composition per liter: CoCl2·6H2O ................................................................................ 45.0g CuSO4·5H2O ............................................................................... 25.0g Na2MoO4·4H2O .......................................................................... 25.0g MnSO4·2H2O .............................................................................. 2.28g

Thermus Medium Enhance

ZnSO4·7H2O ................................................................................. 0.5g H3BO3 ........................................................................................... 0.5g H2SO4 .........................................................................................0.5mL

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Phosphate Buffer: Composition per liter: Na2HPO4·12H2O......................................................................... 43.0g KH2PO4 ....................................................................................... 5.44g

Preparation of Phosphate Buffer: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Preparation of Medium: Add components, except phosphate buffer, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Add 100.0mL warm phosphate buffer. Mix thoroughly. Pour into Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Thermus spp., Rubrobacter xylanophilus, Thermonema rossianum, Deinococcus geothermalis, Deinococcus murrayi, and Tepidimonas ignava.

Thermus 162 Medium Composition per 1010.0mL:

1747

Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Adjust pH to 7.0. Preparation of Medium: Add components, except phosphate buffer solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.2 with NaOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°– 55°C. Aseptically add 100.0mL of sterile phosphate buffer solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Thermus species.

Thermus Medium Enhanced Composition per liter: Agar ............................................................................................ 28.0g Yeast extract.................................................................................. 2.5g Tryptone........................................................................................ 2.5g MgCl2·6H2O ................................................................................. 0.2g Nitrilotriacetic acid ....................................................................... 0.1g CaSO4·2H2O ............................................................................... 0.04g Phosphate buffer solution ......................................................100.0mL Ferric citrate solution.................................................................0.5mL Trace elements solution .............................................................0.5mL

Agar ............................................................................................ 28.0g Tryptone ........................................................................................ 2.5g Yeast extract.................................................................................. 2.5g MgCl2·6H2O.................................................................................. 0.2g Nitrilotriacetic acid ....................................................................... 0.1g CaSO4·2H2O ............................................................................ 40.0mg Phosphate buffer solution ......................................................100.0mL Ferric citrate solution .................................................................0.5mL Trace elements solution .............................................................0.5mL pH 7.2 ± 0.2 at 25°C

Ferric Citrate Solution: Composition per 100.0mL:

Phosphate Buffer Solution: Composition per liter:

Preparation of Phosphate Buffer Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure–121°C.

Na2HPO4·12H2O......................................................................... 43.0g KH2PO4 ....................................................................................... 5.44g

Preparation of Phosphate Buffer Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure–121°C.

Ferric Citrate Solution: Composition per 10.0mL: Ferric citrate ............................................................................. 24.5mg

Preparation of Ferric Citrate Solution: Add ferric citrate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Trace Elements Solution: Composition per liter: Nitrilotriacetic acid ..................................................................... 12.8g FeCl2·4H2O ................................................................................... 1.0g MnCl2·4H2O.................................................................................. 0.5g CoCl2·4H2O .................................................................................. 0.3g CuCl2·2H2O ............................................................................. 50.0mg Na2MoO4·2H2O ....................................................................... 50.0mg H3BO3 ...................................................................................... 20.0mg NiCl2·6H2O .............................................................................. 20.0mg © 2010 by Taylor and Francis Group, LLC

Ferric citrate................................................................................ 0.24g

Preparation of Ferric Citrate Solution: Add ferric citrate to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Trace Elements Solution: Composition per liter: Nitrilotriacetic acid ..................................................................... 12.8g FeCl3·4H2O................................................................................... 1.0g MnCl2·4H2O ................................................................................. 0.5g CoCl2·6H2O .................................................................................. 0.3g CuCl2·2H2O ................................................................................ 0.05g Na2MoO4·2H2O .......................................................................... 0.05g H3BO3 ......................................................................................... 0.02g NiCl2·6H2O................................................................................. 0.02g

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium: Add components, except phosphate buffer solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0mL of sterile phosphate buffer solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Thermus species.

1748

Thermus Medium Enhanced with 1% NaCl

Thermus Medium Enhanced with 1% NaCl Composition per liter: Agar ............................................................................................ 28.0g NaCl ............................................................................................ 10.0g Yeast extract.................................................................................. 2.5g Tryptone ........................................................................................ 2.5g MgCl2·6H2O.................................................................................. 0.2g Nitrilotriacetic acid ....................................................................... 0.1g CaSO4·2H2O ............................................................................... 0.04g Phosphate buffer solution ......................................................100.0mL Ferric citrate solution .................................................................0.5mL Trace elements solution .............................................................0.5mL

Ferric Citrate Solution: Composition per 100.0mL: Ferric citrate ................................................................................ 0.24g

Preparation of Ferric Citrate Solution: Add ferric citrate to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Phosphate Buffer Solution: Composition per liter:

Yeast extract.................................................................................. 1.5g NaCl.............................................................................................. 1.5g pH 7.0 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Thermus aquaticus and other Thermus species.

Thermus PMY Broth (Thermus Peptone Meat Extract Yeast Extract Broth) Composition per liter: Peptone ......................................................................................... 5.0g Meat extract .................................................................................. 3.5g Agar .............................................................................................. 3.0g Yeast extract.................................................................................. 1.5g NaCl.............................................................................................. 1.5g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

Na2HPO4·12H2O......................................................................... 43.0g KH2PO4 ....................................................................................... 5.44g

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Phosphate Buffer Solution: Add components to

Use: For the cultivation and maintenance of Thermus aquaticus and other Thermus species.

distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure–121°C.

Trace Elements Solution: Composition per liter: Nitrilotriacetic acid ..................................................................... 12.8g FeCl3·4H2O ................................................................................... 1.0g MnCl2·4H2O.................................................................................. 0.5g CoCl2·6H2O .................................................................................. 0.3g CuCl2·2H2O ................................................................................ 0.05g Na2MoO4·2H2O .......................................................................... 0.05g H3BO3 ......................................................................................... 0.02g NiCl2·6H2O ................................................................................. 0.02g

Use: For the cultivation and maintenance of Rhodothermus marinus. Thermus Peptone Meat Extract Yeast Extract Agar See: Thermus PMY Agar

Thermus ruber Medium Composition per liter: Agar ............................................................................................ 12.0g Universal peptone ......................................................................... 5.0g Starch, soluble............................................................................... 1.0g Yeast extract.................................................................................. 1.0g pH 8.0 ± 0.2 at 25°C

Source: Universal peptone is available from Merck, Sharpe, and Dohme. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Thermus ruber.

Thermus sp. Medium (DSMZ Medium 1045) Composition per liter: Peptone ........................................................................................ 8.0g Yeast extract ................................................................................. 4.0g NaCl ............................................................................................. 2.0g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

Thermus Peptone Meat Extract Yeast Extract Broth See: Thermus PMY Broth

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Thermus PMY Agar (Thermus Peptone Meat Extract Yeast Extract Agar)

Thermus thermophilus Medium (DSMZ Medium 74)

Composition per liter: Agar ............................................................................................ 15.0g Peptone.......................................................................................... 5.0g Meat extract .................................................................................. 3.5g © 2010 by Taylor and Francis Group, LLC

Use: For the cultivation of Thermus spp.

Polypeptone™ .............................................................................. 8.0g Yeast extract.................................................................................. 4.0g NaCl.............................................................................................. 2.0g pH 7.0 ± 0.2 at 25°C

Thiazole Medium Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation and maintenance of Thermus thermophilus.

Thiamine Assay Medium Composition per liter: Glucose ....................................................................................... 40.0g Peptone........................................................................................ 22.0g Sodium acetate ............................................................................ 15.0g Vitamin assay casamino acids....................................................... 5.0g K2HPO4 ......................................................................................... 1.0g KH2PO4 ......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.4g Adenine sulfate ........................................................................... 0.02g FeSO4·7H2O................................................................................ 0.02g Guanine·HCl ............................................................................... 0.02g MnSO4·5H2O .............................................................................. 0.02g NaCl ............................................................................................ 0.02g Uracil .......................................................................................... 0.02g L-Cystine .................................................................................... 0.2mg p-Aminobenzoic acid................................................................. 0.2mg Calcium pantothenate ................................................................ 0.2mg Niacin......................................................................................... 0.2mg Pyridoxine·HCl .......................................................................... 0.2mg Riboflavin .................................................................................. 0.2mg Folic acid.....................................................................................0.5μg Biotin ..........................................................................................0.8μg pH 6.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Continue boiling for 2–3 min. Distribute into tubes or flasks in 5.0mL volumes while swirling the flask to disperse the precipitate. Add standard solutions or test solutions and bring volume of each tube to 10.0mL with distilled/deionized water. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the microbiological assaying of thiamine using Lactobacillus fermentum as the test organism.

Thiamine Assay Medium LV Composition per liter: Glucose ....................................................................................... 20.0g Pancreatic digest of casein .......................................................... 20.0g K2HPO4 ....................................................................................... 10.0g NaCl ............................................................................................ 10.0g Sodium citrate ............................................................................. 10.0g Yeast extract, thiamine-free ........................................................ 10.0g Sorbitan monooleate complex ...................................................... 2.0g MgSO4·7H2O ................................................................................ 1.6g MnCl2·4H2O................................................................................ 0.28g FeSO4·7H2O................................................................................ 0.08g pH 6.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

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to boiling. Continue boiling for 2–3 min. Distribute into tubes or flasks in 5.0mL volumes while swirling the flask to disperse the precipitate. Add standard solutions or test solutions and bring volume of each tube to 10.0mL with distilled/deionized water. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the microbiological assaying of thiamine using Lactobacillus viridescens as the test organism.

Thiamine Salts Medium Composition per liter: KH2PO4......................................................................................... 1.0g FeSO4·7H2O................................................................................ 0.05g MgSO4·7H2O .............................................................................. 0.02g CaCl2 ........................................................................................... 0.02g MnCl2·4H2O .............................................................................. 1.0mg Na2MoO4·2H2O ......................................................................... 1.0mg Thiamine·HCl solution ............................................................10.0mL pH 7.0 ± 0.2 at 25°C

Thiamine·HCl Solution: Composition per 10.0mL: Thiamine·HCl ............................................................................... 3.0g

Preparation of Thiamine·HCl Solution: Add thiamine·HCl to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except thiamine·HCl solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Adjust pH to 7.0 with KOH. Autoclave for 20 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add the sterile thiamine·HCl solution. Mix thoroughly. Distribute into sterile tubes or flasks.

Use: For the cultivation of ATCC strain 25589.

Thiazole Medium Composition per 110.0mL: Solution A..............................................................................100.0mL Solution B ..................................................................................5.0mL Solution C ..................................................................................5.0mL pH 7.0 ± 0.2 at 25°C

Solution A: Composition per 100.0mL: Benzothiazole .......................................................................... 30.0mg NaOH (10% solution) ..............................................................90.0mL

Preparation of Solution A: Combine components. Mix thoroughly. Adjust pH to 7.0 with concentrated HCl. Autoclave for 15 min at 15 psi pressure–121°C.

Solution B: Composition per 50.0mL: KH2PO4......................................................................................... 0.3g

Preparation of Solution B: Add KH2PO4 to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Solution C: Composition per 50.0mL: MgSO4·7H2O ........................................................................... 50.0mg FeCl3 ........................................................................................ 10.0mg CaCl2·2H2O ............................................................................... 6.0mg

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Thioalkalivibrio halophilus Medium

Preparation of Solution C: Add components to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Preparation of Medium: Aseptically combine 100.0mL of sterile solution A with 5.0mL of sterile solution B and 5.0mL of sterile solution C. Mix thoroughly.

Use: For the cultivation of unidentified bacteria DSMZ 8993 and DSMZ 8994.

THIO Medium See: Thioglycolate Medium, Enriched

Preparation of Trace Elements Solution SL-4: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Trace Elements Solution SL-6: Composition per liter: H3BO3 ........................................................................................... 0.3g CoCl2·6H2O .................................................................................. 0.2g ZnSO4·7H2O ................................................................................. 0.1g MnCl2·4H2O ............................................................................... 0.03g Na2MoO4·H2O ............................................................................ 0.03g NiCl2·6H2O ................................................................................. 0.02g CuCl2··2H2O................................................................................ 0.01g

THIO + Bile Medium See: Thioglycolate Medium with 20% Bile

Preparation of Trace Elements Solution SL-6: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3.4.

Thioalkalivibrio halophilus Medium (DSMZ Medium 1014)

Preparation of Medium: Add components, except magnesium chloride, magnesium sulfate, thiosulfate, and bicarbonate solutions, to distilled/deionized water and bring volume to 960.0mL. Mix thoroughly. Adjust pH to 8.0–8.5. Dispense into screw-capped Erlenmeyer flasks (fill to 1/10 volume). Autoclave for 30 min at 6 psi pressure– 110°C. Cool to room temperature. Aseptically add magnesium chloride, magnesium sulfate, thiosulfate, and bicarbonate solutions. Mix thoroughly.

Composition per liter: NaCl ......................................................................................... 175.0g K2HPO4 ......................................................................................... 1.5g NH4Cl ........................................................................................... 0.5g Magnesium chloride solution...................................................10.0mL Magnesium sulfate solution .....................................................10.0mL Thiosulfate solution .................................................................10.0mL Bicarbonate solution ................................................................10.0mL Trace element solution SL-4 ......................................................1.0mL pH 8.2 ± 0.2 at 25°C

Magnesium Sulfate Solution: Composition per 10.0mL: MgSO4·7H2O .............................................................................. 0.25g

Preparation of Magnesium Sulfate Solution: Add MgSO4·7H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Magnesium Chloride Solution: Composition per 10.0mL: MgCl2·6H2O.................................................................................. 0.2g

Preparation of Magnesium Chloride Solution: Add MgCl2·6H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Thiosulfate Solution: Composition per 10.0mL: Na2S2O3·5H2O .............................................................................. 5.0g

Preparation of Thiosulfate Solution: Add Na2S2O3·5H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Adjust to pH 10.0. Filter sterilize. Bicarbonate Solution: Composition per 10.0mL: NaHCO3 ........................................................................................ 4.0g

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Trace Elements Solution SL-4: Composition per liter: EDTA ............................................................................................ 0.5g FeSO4·7H2O.................................................................................. 0.2g Trace elements solution SL-6 ..................................................... 100.0 © 2010 by Taylor and Francis Group, LLC

Use: For the cultivation of Thioalkalivibrio halophilus.

Thiobacillus A2 Agar (T3 Agar) Composition per 1100.0mL: Solution B .....................................................................................1.0L Solution A..............................................................................100.0mL pH 8.5 ± 0.2 at 25°C

Solution A: Composition per 100.0mL: Na2S2O3·5H2O .............................................................................. 5.0g Na2HPO4 ....................................................................................... 4.2g KH2PO4......................................................................................... 1.5g NH4Cl ........................................................................................... 1.0g Phenol Red (0.2% solution).......................................................1.0mL

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 9.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Solution B: Composition per liter: Agar ............................................................................................ 15.0g MgSO4·7H2O ................................................................................ 0.1g Trace metal solution...................................................................5.0mL

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 1.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Trace Metal Solution: Composition per liter: EDTA .......................................................................................... 50.0g ZnSO4 ......................................................................................... 22.0g CaCl2 ........................................................................................... 5.54g MnCl2.......................................................................................... 5.06g FeSO4·7H2O................................................................................ 4.99g CoCl2 .......................................................................................... 1.61g CuSO4 ......................................................................................... 1.57g (NH4)2MoO4·4H2O ....................................................................... 1.1g

Thiobacillus acidophilus Broth Preparation of Trace Metal Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0 with KOH.

Preparation of Medium: Aseptically add 100.0mL of sterile solution A to 1.0L of sterile solution B. Mix thoroughly. Adjust pH to 8.5 if necessary. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation and maintenance of Thiobacillus versutus and other Thiobacillus species.

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KCl................................................................................................ 0.1g Ca(NO3)2·4H2O ....................................................................... 18.0mg FeSO4·7H2O............................................................................. 0.01mg Glucose solution ......................................................................20.0mL pH 4.5 ± 0.2 at 25°C

Glucose Solution: Composition per 20.0mL: Glucose ....................................................................................... 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deion-

Thiobacillus A2 Broth (T3 Broth)

ized water and bring volume to 20.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Composition per 1100.0mL:

Preparation of Medium: Add components, except glucose solu-

Solution B .....................................................................................1.0L Solution A ..............................................................................100.0mL pH 8.5 ± 0.2 at 25°C

tion, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Adjust pH to 4.5 with H2SO4. Aseptically add 20.0mL of sterile glucose solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Solution A: Composition per 100.0mL: Na2S2O3·5H2O .............................................................................. 5.0g Na2HPO4 ....................................................................................... 4.2g KH2PO4 ......................................................................................... 1.5g NH4Cl ........................................................................................... 1.0g Phenol Red (0.2% solution) .......................................................1.0mL

Use: For the cultivation and maintenance of Thiobacillus acidophilus.

Thiobacillus acidophilus Broth Composition per liter:

MgSO4·7H2O ................................................................................ 0.1g Trace metal solution...................................................................5.0mL

Glucose ....................................................................................... 10.0g (NH4)2SO4 .................................................................................... 3.0g MgSO4·7H2O ............................................................................... 1.0g KH2PO4......................................................................................... 0.5g KCl................................................................................................ 0.1g Ca(NO3)2·4H2O ....................................................................... 18.0mg FeSO4·7H2O............................................................................. 0.01mg pH 3.5 ± 0.2 at 25°C

Preparation of Solution B: Add components to distilled/deionized

Preparation of Medium: Add components to distilled/deionized

Solution B: Composition per liter:

water and bring volume to 1.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Trace Metal Solution: Composition per liter: EDTA .......................................................................................... 50.0g ZnSO4 ......................................................................................... 22.0g CaCl2 ........................................................................................... 5.54g MnCl2 .......................................................................................... 5.06g FeSO4·7H2O................................................................................ 4.99g CoCl2........................................................................................... 1.61g CuSO4 ......................................................................................... 1.57g (NH4)2MoO4·4H2O ....................................................................... 1.1g

Preparation of Trace Metal Solution: Add components to distilled/deionized water and bring volume to 1.0mL. Mix thoroughly. Adjust pH to 6.0 with KOH.

Preparation of Medium: Aseptically add 100.0mL of sterile solution A to 1.0L of sterile solution B. Mix thoroughly. Adjust pH to 8.5 if necessary. Distribute into sterile tubes or flasks. Use: For the cultivation and maintenance of Thiobacillus versutus and other Thiobacillus species.

Thiobacillus acidophilus Agar Composition per liter: Agar ............................................................................................ 15.0g (NH4)2SO4 ..................................................................................... 3.0g MgSO4·7H2O ............................................................................... 1.0g KH2PO4 ......................................................................................... 0.5g © 2010 by Taylor and Francis Group, LLC

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3.5 with H2SO4. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Thiobacillus acidophilus.

Thiobacillus acidophilus Medium (DSMZ Medium 108) Composition per liter: Agar ............................................................................................ 15.0g (NH4)2SO4 .................................................................................... 3.0g MgSO4·7H2O ................................................................................ 1.0g KH2PO4......................................................................................... 0.5g KCl............................................................................................... 0.1g Ca(NO3)2·4H2O ....................................................................... 18.0mg FeSO4·7H2O............................................................................. 0.01mg Glucose solution ......................................................................50.0mL pH 4.5 ± 0.2 at 25°C

Glucose Solution: Composition per 100.0mL: D-Glucose .................................................................................... 10.0g

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Thiobacillus acidophilus Medium

sterile glucose solution. Mix thoroughly. Adjust pH to 4.5 with H2SO4. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Acidiphilium acidophilum.

Thiobacillus acidophilus Medium (DSMZ Medium 108)

(NH4)2·SO4 ................................................................................... 0.1g MgSO4·7H2O ................................................................................ 0.1g CaCl2 ............................................................................................. 0.1g FeCl3·6H2O ................................................................................ 2.0mg MnSO4·4H2O ............................................................................. 2.0mg pH 6.6 ± 0.2 at 25°C

Source: Ionagar No. 2 is available from Oxoid Unipath.

Composition per liter:

Preparation of Medium: Add components, except the agar, to dis-

(NH4)2SO4 ..................................................................................... 3.0g MgSO4·7H2O ................................................................................ 1.0g KH2PO4 ......................................................................................... 0.5g KCl............................................................................................... 0.1g Ca(NO3)2·4H2O........................................................................ 18.0mg FeSO4·7H2O............................................................................. 0.01mg Glucose solution ......................................................................50.0mL pH 3.5 ± 0.2 at 25°C

Use: For the cultivation of nonaciduric Thiobacillus species such as Thiobacillus neapolitanus, Thiobacillus novellus, and Thiobacillus thioparus.

Glucose Solution: Composition per 100.0mL: D-Glucose .................................................................................... 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except glucose solution, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 50.0mL sterile glucose solution. Mix thoroughly. Adjust pH to 3.5 with H2SO4. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Acidiphilium acidophilum.

Thiobacillus Agar Composition per liter: Agar, noble.................................................................................. 20.0g Na2S2O3·5H2O .............................................................................. 5.0g KH2PO4 ......................................................................................... 3.0g CaCl2 ............................................................................................. 0.1g MgCl2·6H2O.................................................................................. 0.1g (NH4)2·Cl ...................................................................................... 0.1g pH 4.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except agar, to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 4.2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Add agar to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically combine 500.0mL of the sterile basal medium with 500.0mL of the sterile agar solution. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of aciduric Thiobacillus species such as Thiobacillus concretivorus and Thiobacillus intermedius.

Thiobacillus Agar Composition per liter: Ionagar No. 2 .............................................................................. 12.0g Na2S2O3·5H2O ............................................................................ 10.0g K2HPO4 ......................................................................................... 4.0g KH2PO4 ......................................................................................... 4.0g © 2010 by Taylor and Francis Group, LLC

tilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Adjust the pH to 6.6. Add the agar. Bring volume to 1.0L with distilled/ deionized water. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 20 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Thiobacillus Agar I for Acidophilic Thiobacillus Composition per liter: Solution A..............................................................................500.0mL Solution B ..............................................................................500.0mL pH 4.2 ± 0.2 at 25°C

Solution A: Composition per 500.0mL: Na2S2O3 ........................................................................................ 5.0g KH2PO4 ........................................................................................ 3.0g CaCl2 ............................................................................................ 0.1g MgCl2·6H2O ................................................................................. 0.1g NH4Cl ........................................................................................... 0.1g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 500.0mL. Adjust pH to 4.2 with 1N HCl. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.

Solution B: Composition per 500.0mL: Agar, noble.................................................................................. 20.0g

Preparation of Solution A: Add agar to distilled/deionized water and bring volume to 500.0mL. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 50°C.

Preparation of Medium: Aseptically combine 500.0mL of sterile solution A and 500.0mL of sterile solution B. Combine the two solutions while still hot. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation of Thiobacillus thiooxidans.

Thiobacillus albertis Agar Composition per 500.0mL: Solution A..............................................................................250.0mL Solution B ..............................................................................250.0mL pH 4.0 ± 0.2 at 25°C

Solution A: Composition per 250.0mL: Na2S2O3·5H2O .............................................................................. 5.0g KH2PO4......................................................................................... 3.0g MgSO4·7H2O ................................................................................ 0.5g (NH4)2SO4 .................................................................................... 0.4g CaCl2·2H2O ................................................................................ 0.25g FeSO4·7H2O............................................................................ 10.0mg

Thiobacillus aquaesulis Broth Preparation of Solution A: Add components to distilled/deionized water and bring volume to 250.0mL. Mix thoroughly. Adjust pH to 4.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Solution B: Composition per 250.0mL: Agar ............................................................................................ 15.0g

Preparation of Solution B: Add agar to distilled/deionized water and bring volume to 250.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Preparation of Medium: Aseptically combine 250.0mL of sterile solution A with 250.0mL of sterile solution B. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Thiobacillus albertis.

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MnCl2·4H2O ................................................................................. 2.5g CoCl2·6H2O .................................................................................. 0.5g (NH4)6MoO24·4H2O...................................................................... 0.5g CuSO4·5H2O................................................................................. 0.2g

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0. Preparation of Solution B: Add components to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Preparation of Medium: Aseptically combine 100.0mL of cooled, sterile solution A with 900.0mL of cooled, sterile solution B. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Thiobacillus aquaesulis.

Thiobacillus albertis Broth Composition per liter: Na2S2O3·5H2O .............................................................................. 5.0g KH2PO4 ......................................................................................... 3.0g MgSO4·7H2O ................................................................................ 0.5g (NH4)2SO4 ..................................................................................... 0.4g CaCl2·2H2O................................................................................. 0.25g FeSO4·7H2O............................................................................ 10.0mg pH 4.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 4.5. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation of Thiobacillus albertis.

Thiobacillus aquaesulis Agar Composition per liter: Solution B ..............................................................................900.0mL Solution A ..............................................................................100.0mL pH 7.4 ± 0.2 at 25°C

Solution A: Composition per 100.0mL: Na2HPO42H2O.............................................................................. 7.9g KH2PO4 ......................................................................................... 1.5g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 7.4. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Solution B: Composition per 900.0mL: Agar ............................................................................................ 15.0g Na2S2O3 ........................................................................................ 5.0g NH4Cl ........................................................................................... 0.4g MgSO4·7H2O ................................................................................ 0.1g Phenol Red ................................................................................. 3.0mg Trace elements solution ...........................................................10.0mL

Thiobacillus aquaesulis Broth Composition per liter: Solution B ..............................................................................900.0mL Solution A..............................................................................100.0mL pH 7.4 ± 0.2 at 25°C

Solution A: Composition per 100.0mL: Na2HPO42H2O.............................................................................. 7.9g KH2PO4......................................................................................... 1.5g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 7.4. Autoclave for 15 min at 15 psi pressure–121°C. Solution B: Composition per 900.0mL: Na2S2O3 ........................................................................................ 5.0g NH4Cl ........................................................................................... 0.4g MgSO4·7H2O ................................................................................ 0.1g Phenol Red................................................................................. 3.0mg Trace elements solution ...........................................................10.0mL

Trace Elements Solution: Composition per liter: Disodium EDTA ......................................................................... 50.0g NaOH......................................................................................... 11.0g ZnSO4·7H2O ............................................................................... 11.0g CaCl2·2H2O ................................................................................ 7.34g FeSO4·7H2O.................................................................................. 5.0g MnCl2·4H2O ................................................................................. 2.5g CoCl2·6H2O .................................................................................. 0.5g (NH4)6MoO24·4H2O...................................................................... 0.5g CuSO4·5H2O................................................................................. 0.2g

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0. Preparation of Solution B: Add components to distilled/deionized water and bring the volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Aseptically combine 100.0mL of cooled, sterile solution A with 900.0mL of cooled, sterile solution B. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Thiobacillus aquaesulis.

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Thiobacillus aquaesulis Medium

Thiobacillus caldus Agar

Composition per liter:

Solution B ..............................................................................900.0mL Solution A ..............................................................................100.0mL

Solution E ..............................................................................500.0mL Solution A..............................................................................460.0mL Solution D................................................................................20.0mL Solution B ................................................................................10.0mL Solution C ................................................................................10.0mL pH 2.5 ± 0.2 at 25°C

Solution A: Composition per 100.0mL: Na2HPO4·2H2O............................................................................. 7.9g KH2HPO4 ...................................................................................... 1.5g

Solution B: Composition per 900.0mL: Agar ............................................................................................ 15.0g Na2S2O3 ........................................................................................ 5.0g NH4Cl ........................................................................................... 0.4g MgSO4·7H2O ................................................................................ 0.1g Phenol Red ................................................................................. 3.0mg Trace metals .............................................................................10.0mL

Trace Metals: Composition per liter: EDTA .......................................................................................... 50.0g NaOH .......................................................................................... 11.0g ZnSO4·7H2O ............................................................................... 11.0g CaCl2·2H2O................................................................................. 7.34g FeSO4·7H2O.................................................................................. 5.0g MnCl2·4H2O.................................................................................. 2.5g CoCl2·6H2O .................................................................................. 0.5g (NH4)2Mo2O27 .............................................................................. 0.5g CuSO4·5H2O ................................................................................. 0.2g

Preparation of Trace Metals: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0 with H2SO4.

Preparation of Medium: Aseptically combine 100.0mL of sterile solution A with 900.0mL of sterile solution B. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Thiobacillus aquaesulis.

Thiobacillus Broth I for Acidophilic Thiobacillus

Solution A: Composition per 460.0mL: Na2SO4·10H2O ............................................................................. 3.2g (NH4)2SO4 .................................................................................... 3.0g MgSO4·7H2O ................................................................................ 0.5g KCl................................................................................................ 0.1g K2HPO4.................................................................................... 50.0mg

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 460.0mL. Mix thoroughly. Adjust pH to 1.75 with H2SO4. Autoclave for 15 min at 15 psi pressure–121°C. Cool and maintain above 60°C.

Solution B: Composition per 10.0mL: FeCl3·6H2O.............................................................................. 11.0mg Ca(NO3)2·4H2O....................................................................... 10.0mg H3BO3 ........................................................................................ 2.0mg MnSO4·H2O............................................................................... 2.0mg ZnSO4·7H2O.............................................................................. 0.9mg Na2MoO4·2H2O......................................................................... 0.8mg CoCl2·6H2O............................................................................... 0.6mg CuSO4·5H2O.............................................................................. 0.5mg

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Solution C: Composition per 10.0mL: Glucose ....................................................................................... 0.45g

Preparation of Solution C: Add glucose to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Solution D: Composition per 20.0mL: Na2S4O6 ...................................................................................... 0.77g

Preparation of Solution D: Add Na2S4O6 to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize. Solution E: Composition per 500.0mL:

Composition per liter:

Phytagel™ (Gellan gum; available from Sigma Chemical Co.) 15.0g

Na2S2O3 ........................................................................................ 5.0g KH2PO4 ........................................................................................ 3.0g CaCl2 ............................................................................................. 0.1g MgCl2·6H2O ................................................................................. 0.1g NH4Cl ........................................................................................... 0.1g pH 4.2 ± 0.2 at 25°C

Preparation of Solution E: Add phytagel to distilled/deionized

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Adjust pH to 4.2 with 1N HCl. Mix thoroughly. Distribute into screw-capped tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool and maintain above 60°C.

Preparation of Medium: Maintain solutions A and E above 60°C to prevent rapid gelling of medium. Aseptically combine 460.0mL of sterile solution A with 10.0mL of sterile solution B, 10.0mL of sterile solution C, 20.0mL of sterile solution D, and 500.0mL of sterile solution E. Mix throughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the heterotrophic cultivation and maintenance of Thiobacillus caldus.

Thiobacillus caldus Medium

Thiobacillus caldus Broth Composition per liter:

1755

KCl................................................................................................ 0.1g K2HPO4.................................................................................... 50.0mg

Solution A ..............................................................................960.0mL Solution D ................................................................................20.0mL Solution B ................................................................................10.0mL Solution C ................................................................................10.0mL pH 2.5 ± 0.2 at 25°C

Preparation of Solution A: Add components to distilled/deionized

Solution A: Composition per 960.0mL:

FeCl3·6H2O.............................................................................. 11.0mg Ca(NO3)2·4H2O....................................................................... 10.0mg H3BO3 ........................................................................................ 2.0mg MnSO4·H2O............................................................................... 2.0mg ZnSO4·7H2O.............................................................................. 0.9mg Na2MoO4·2H2O......................................................................... 0.8mg CoCl2·6H2O............................................................................... 0.6mg CuSO4·5H2O.............................................................................. 0.5mg

Na2SO4·10H2O.............................................................................. 3.2g (NH4)2SO4 ..................................................................................... 3.0g MgSO4·7H2O ................................................................................ 0.5g KCl................................................................................................ 0.1g K2HPO4 .................................................................................... 50.0mg

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 960.0mL. Mix thoroughly. Adjust pH to 1.75 with H2SO4. Autoclave for 15 min at 15 psi pressure–121°C.

Solution B: Composition per 10.0mL: FeCl3·6H2O.............................................................................. 11.0mg Ca(NO3)2·4H2O ....................................................................... 10.0mg H3BO3 ........................................................................................ 2.0mg MnSO4·H2O............................................................................... 2.0mg ZnSO4·7H2O .............................................................................. 0.9mg Na2MoO4·2H2O......................................................................... 0.8mg CoCl2·6H2O ............................................................................... 0.6mg CuSO4·5H2O.............................................................................. 0.5mg

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Solution C: Composition per 10.0mL: Glucose ....................................................................................... 0.45g

Preparation of Solution D: Add Na2S4O6 to distilled/deionized

water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Aseptically combine 960.0mL of sterile solution A with 10.0mL of sterile solution B, 10.0mL of sterile solution C, and 20.0mL of sterile solution D. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the heterotrophic cultivation and maintenance of Thiobacillus caldus.

Thiobacillus caldus Broth Composition per liter: Solution A ..............................................................................970.0mL Solution C ................................................................................20.0mL Solution B ................................................................................10.0mL pH 2.5 ± 0.2 at 25°C

Solution A: Composition per 970.0mL: Na2SO4·10H2O.............................................................................. 3.2g (NH4)2SO4 ..................................................................................... 3.0g MgSO4·7H2O ................................................................................ 0.5g © 2010 by Taylor and Francis Group, LLC

water and bring volume to 970.0mL. Mix thoroughly. Adjust pH to 1.75 with H2SO4. Autoclave for 15 min at 15 psi pressure–121°C.

Solution B: Composition per 10.0mL:

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Solution C: Composition per 20.0mL: Na2S4O6 ...................................................................................... 0.77g

Preparation of Solution C: Add Na2S4O6 to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Aseptically combine 970.0mL of sterile solution A with 10.0mL of sterile solution B and 20.0mL of sterile solution C. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the chemolithotrophic cultivation of Thiobacillus caldus.

Thiobacillus caldus Medium Composition per liter: Solution E ..............................................................................500.0mL Solution A..............................................................................460.0mL Solution D................................................................................20.0mL Solution B ................................................................................10.0mL Solution C ................................................................................10.0mL pH 2.5 ± 0.2 at 25°C

Solution B: Composition per 10.0mL: FeCl3·6H2O.............................................................................. 11.0mg Ca(NO3)2·4H2O ....................................................................... 10.0mg H3BO3 ........................................................................................ 2.0mg MnSO4·H2O ............................................................................... 2.0mg ZnSO4·7H2O .............................................................................. 0.9mg Na2MoO4·2H2O ......................................................................... 0.8mg CoCl2·6H2O ............................................................................... 0.6mg CuSO4·5H2O.............................................................................. 0.5mg

1756

Thiobacillus cuprinus Medium

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Warm to 60°C.

NiCl2·6H2O ............................................................................... 0.025g Na2SeO3·5H2O........................................................................... 0.3mg

Solution C: Composition per 10.0mL:

acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L.

Glucose ....................................................................................... 0.45g

Preparation of Solution C: Add glucose to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Warm to 60°C. Solution D: Composition per 20.0mL:

Preparation of Trace Elements Solution: Add nitrilotriacetic

Preparation of Medium: Add components, except sulfur, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3.5 with H2SO4. Autoclave for 15 min at 15 psi pressure– 121°C. Aseptically add 0.5g of sterile sulfur. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Sodium tetrathionate ................................................................... 0.77g

Use: For the cultivation and maintenance of Thiobacillus cuprinus.

Preparation of Solution D: Add sodium tetrathionate to distilled/ deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize. Warm to 60°C.

Composition per liter:

Solution E: Composition per 500.0mL: Phytagel ...................................................................................... 15.0g

Preparation of Solution E: Add phytagel to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 60°–65°C. Preparation of Medium: Aseptically combine 460.0mL of sterile solution A, 10.0mL of sterile solution B, 10.0mL of sterile solution C, 20.0mL of sterile solution D, and 500.0mL of sterile solution E. Mix thoroughly. Aseptically pour into sterile Petri dishes or distrbute into sterile tubes.

Use: For the cultivation of Thiobacillus caldus.

Thiobacillus cuprinus Medium Composition per 1010.0mL: MgSO4·7H2O .............................................................................. 3.45g MgCl2·6H2O................................................................................ 2.75g NH4Cl ......................................................................................... 1.25g NaCl .............................................................................................. 0.5g Sulfur, powdered........................................................................... 0.5g KCl.............................................................................................. 0.33g CaCl2·2H2O................................................................................. 0.14g K2HPO4 ....................................................................................... 0.14g NiCl2·6H2O ................................................................................ 2.0mg Trace elements solution ...........................................................10.0mL pH 3.5 ± 0.2 at 25°C

Preparation of Sulfur: Sterilize 1.0g of powdered sulfur by steaming for 1 hr on three consecutive days.

Trace Elements Solution: Composition per liter: MgSO4·7H2O ............................................................................... 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl .............................................................................................. 1.0g MnSO4·2H2O ............................................................................... 0.5g CoSO4·7H2O ............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O................................................................................... 0.1g FeSO4·7H2O.................................................................................. 0.1g KAI(SO4)2·12H2O....................................................................... 0.02g CuSO4·5H2O ............................................................................... 0.01g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O ......................................................................... 0.01g © 2010 by Taylor and Francis Group, LLC

Thiobacillus denitrificans Medium KNO3 ............................................................................................ 5.0g Na2S2O3·5H2O .............................................................................. 5.0g NaHCO3 ........................................................................................ 1.0g K2HPO4......................................................................................... 0.2g MgCl2............................................................................................ 0.1g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Thiobacillus denitrificans.

Thiobacillus denitrificans Medium Composition per liter: Na2S2O3·5H2O .............................................................................. 5.0g KNO3 ............................................................................................ 2.0g KH2PO4......................................................................................... 2.0g NaHCO3 ........................................................................................ 2.0g NH4Cl ........................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.8g Trace metals solution .................................................................1.0mL pH 6.8–7.0 at 25°C

Trace Metals Solution: Composition per liter: Disodium EDTA ......................................................................... 50.0g NaOH.......................................................................................... 11.0g CaCl2·2H2O ................................................................................ 7.34g FeSO4·7H2O.................................................................................. 5.0g MnCl2·2H2O ................................................................................. 2.5g ZnSO4·7H2O ................................................................................. 2.2g CoCl2·6H2O .................................................................................. 0.5g (NH4)6Mo7O24·4H2O .................................................................... 0.5g CuSO4·5H2O ................................................................................. 0.2g

Preparation of Trace Metals Solution: Add EDTA to distilled/ deionized water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 6.0 with NaOH. Add remaining components, one by one. Maintain the pH at 6.0. After dissolution of all the salts, adjust the pH to 4.0 with HCl. Store at 4°C.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of Thiobacillus denitrificans.

Thiobacillus ferrooxidans Medium with Thiosulfate

Thiobacillus ferrooxidans Medium

1757

Thiobacillus ferrooxidans Medium, APH

Composition per liter:

Al2(SO4)3·12H2O .......................................................................... 1.4g NaCl .............................................................................................. 1.0g KH2PO4 ......................................................................................... 0.4g MgSO4·7H2O ................................................................................ 0.1g (NH4)2SO4 ..................................................................................... 0.1g CaCl2 ........................................................................................... 0.03g MnSO4·4H2O .............................................................................. 0.02g FeSO4·7H2O solution.............................................................100.0mL

FeSO4·7H2O................................................................................ 40.0g (NH4)2SO4 .................................................................................... 2.0g K2HPO4......................................................................................... 0.5g MgSO4·7H2O ................................................................................ 0.5g KCl................................................................................................ 0.1g Ca(NO3)2..................................................................................... 0.01g pH 3.0 ± 0.2 at 25°C

FeSO4·7H2O Solution: Composition per 100.0mL:

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3.0 with dilute H2SO4. Distribute into tubes or flasks. Filter sterilize. Aseptically distribute into sterile tubes or flasks.

FeSO4·7H2O................................................................................ 10.0g H2SO4, concentrated ................................................................0.09mL

Preparation of FeSO4·7H2O Solution: Add FeSO4·7H2O and

Preparation of Medium: Add components to distilled/deionized

Use: For the cultivation and maintenance of Leptospirillum ferrooxidans and Thiobacillus ferrooxidans.

H2SO4 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Thiobacillus ferrooxidans Medium with Ferrous Sulfate

Preparation of Medium: Add components, except FeSO4·7H2O

solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into flasks in 90.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 10.0mL of sterile FeSO4·7H2O solution to each flask. Mix thoroughly.

Use: For the cultivation of Thiobacillus ferrooxidans.

Thiobacillus ferrooxidans Medium Composition per liter: Solution I................................................................................400.0mL Solution III .............................................................................400.0mL Solution II ..............................................................................200.0mL

Solution I: Composition per 500.0mL: K2HPO4 ......................................................................................... 0.5g MgSO4·7H2O ................................................................................ 0.5g (NH4)2SO4 ..................................................................................... 0.5g H2SO4 (1N solution) ..................................................................5.0mL

Preparation of Solution I: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Solution II: Composition per liter: FeSO4·7H2O.............................................................................. 167.0g 1N H2SO4 .................................................................................50.0mL

Preparation of Solution II: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Warm to 45°–50°C.

Solution III: Composition per liter: Agar ............................................................................................ 10.0g

Preparation of Solution III: Add agar to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Aseptically combine 400.0mL of sterile solution I, 200.0mL of sterile solution II, and 400.0mL of sterile solution III. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Composition per liter: FeSO4·7H2O................................................................................ 33.3g KH2PO4......................................................................................... 0.4g MgSO4·7H2O ................................................................................ 0.4g (NH4)2SO4 .................................................................................... 0.4g 0.1N H2SO4 ..........................................................................1000.0mL pH 1.4 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 1.4 with H2SO4. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Clostridium acetobutylicum.

Thiobacillus ferrooxidans Medium with Tetrathionate Composition per liter: K2S4O6 ......................................................................................... 5.0g KH2PO4......................................................................................... 3.0g (NH4)2SO4 .................................................................................... 3.0g MgSO4·7H2O ................................................................................ 0.5g CaCl2·2H2O ................................................................................ 0.25g pH 4.4 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 4.4 with H2SO4. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Thiobacillus ferrooxidans.

Thiobacillus ferrooxidans Medium with Thiosulfate Composition per liter: Na2S2O3·5H2O ............................................................................ 5.0g KH2PO4......................................................................................... 3.0g (NH4)2SO4 .................................................................................... 3.0g MgSO4·7H2O ................................................................................ 0.5g CaCl2·2H2O ................................................................................ 0.25g pH 4.4 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 4.4 with

1758

Thiobacillus halophilus Agar

H2SO4. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Thiobacillus ferrooxidans and Thiobacillus thiooxidans.

MnCl2·4H2O ................................................................................. 2.5g CoCl2·6H2O.................................................................................. 0.5g (NH4)6Mo7O24·4H2O ................................................................... 0.5g CuSO4·5H2O................................................................................. 0.2g

Preparation of Trace Metals Solution: Add components to dis-

Thiobacillus halophilus Agar Composition per liter: NaCl ............................................................................................ 50.0g Agar ............................................................................................ 15.0g Na2HPO4·2H2O............................................................................. 7.9g Na2S2O3·5H2O .............................................................................. 5.0g KH2PO4 ......................................................................................... 1.5g NH4Cl ........................................................................................... 0.4g MgSO4·7H2O ................................................................................ 0.1g Phenol Red (saturated aqueous solution) ..............................................12.5mL Trace metals solution ...............................................................10.0mL pH 7.3 ± 0.2 at 25°C

Trace Metals Solution: Composition per liter: Disodium EDTA ......................................................................... 50.0g NaOH .......................................................................................... 11.0g ZnSO4·7H2O ............................................................................... 11.0g CaCl2·2H2O ................................................................................ 7.34g FeSO4·7H2O ................................................................................. 5.0g MnCl2·4H2O ................................................................................. 2.5g CoCl2·6H2O .................................................................................. 0.5g (NH4)6Mo7O24·4H2O ................................................................... 0.5g CuSO4·5H2O................................................................................. 0.2g

Preparation of Trace Metals Solution: Add components to distilled/deionized water and bring volume to 1.0L. Adjust pH to 6.0. Mix thoroughly.

Use: For the cultivation and maintenance of Thiobacillus halophilus.

Thiobacillus halophilus Broth Composition per liter: Solution A ..............................................................................900.0mL Solution B ..............................................................................100.0mL pH 7.3 ± 0.2 at 25°C

Solution A: Composition per 900.0mL: NaCl ............................................................................................ 50.0g Na2S2O3·5H2O .............................................................................. 5.0g NH4Cl ........................................................................................... 0.4g MgSO4·7H2O ................................................................................ 0.1g Phenol Red (saturated aqueous solution).................................12.5mL Trace metals solution ...............................................................10.0mL

tilled/deionized water and bring volume to 1.0L. Adjust pH to 6.0. Mix thoroughly.

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Solution B: Composition per 100.0mL: Na2HPO4·2H2O............................................................................. 7.9g KH2PO4......................................................................................... 1.5g

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Aseptically combine 900.0mL of sterile solution A with 100.0mL of sterile solution B. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation and maintenance of Thiobacillus halophilus.

Thiobacillus halophilus Medium Composition per liter: Solution B ..............................................................................900.0mL Solution A..............................................................................100.0mL pH 7.3 ± 0.2 at 25°C

Solution B: Composition per 900.0mL: NaCl............................................................................................ 50.0g Agar, noble.................................................................................. 15.0g Na2S2O3·5H2O .............................................................................. 5.0g NH4Cl ........................................................................................... 0.4g Phenol Red............................................................................... 10.0mg Hutner's basal salts solution.....................................................20.0mL

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Hutner’s Basal Salts Solution: Composition per liter: MgSO4·7H2O .............................................................................. 29.7g Nitrilotriacetic acid ..................................................................... 10.0g CaCl2·2H2O .............................................................................. 3.335g FeSO4·7H2O............................................................................. 99.0mg (NH4)6MoO7O24·4H2O ............................................................ 9.25mg “Metals 44”..............................................................................50.0mL

“Metals 44”: Composition per 100.0mL: ZnSO4·7H2O ............................................................................. 1.095g FeSO4·7H2O.................................................................................. 0.5g Sodium EDTA............................................................................. 0.25g MnSO4·H2O ............................................................................. 0.154g CuSO4·5H2O ............................................................................ 39.2mg Co(NO3)2·6H2O ....................................................................... 24.8mg Na2B4O7·10H2O....................................................................... 17.7mg

Preparation of “Metals 44”: Add sodium EDTA to distilled/deionized water and bring volume to 90.0mL. Mix thoroughly. Add a few

Thiobacillus intermedius Medium

1759

drops of concentrated H2SO4 to retard precipitation of heavy metal ions. Add remaining components. Mix thoroughly. Bring volume to 100.0mL with distilled/deionized water.

Preparation of Solution II: Add components to distilled/deionized

Preparation of Hutner’s Basal Salts Solution: Add nitrilotria-

II, and 10.0g of Na2S2O3·5H2O. Mix thoroughly. Filter sterilize. Aseptically distribute into sterile tubes or flasks.

cetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Adjust pH to 6.8.

Solution A: Composition per 100.0mL: Na2HPO4 ....................................................................................... 6.3g KH2PO4 ......................................................................................... 1.5g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Preparation of Medium: Aseptically combine 900.0mL of sterile solution B with 100.0mL of sterile solution A. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation of Thiobacillus halophilus.

Thiobacillus Heterotrophic Medium Composition per liter: Glucose ......................................................................................... 5.0g MgSO4·7H2O ................................................................................ 0.5g (NH4)2SO4 ................................................................................... 0.15g KH2PO4 ......................................................................................... 0.1g KCl.............................................................................................. 0.05g Ca(NO3)2 ..................................................................................... 0.01g pH 3.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Use: For the cultivation and maintenance of Thiobacillus organoparus and other heterotrophic Thiobacillus species.

Thiobacillus intermedius Medium Composition per 1010.0mL: Na2S2O3·5H2O ............................................................................ 10.0g Solution I.......................................................................................1.0L Solution II ................................................................................10.0mL

Solution I: Composition per liter: NH4Cl ........................................................................................... 1.0g K2HPO4 ......................................................................................... 0.6g MgCl2·6H2O.................................................................................. 0.5g KH2PO4 ......................................................................................... 0.4g MgSO4 .......................................................................................... 0.3g CaCl2·2H2O................................................................................... 0.2g FeCl3·6H2O ................................................................................. 0.02g

Preparation of Solution I: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Solution II: Composition per liter: CaCl2·2H2O................................................................................... 0.1g ZnSO4·7H2O ............................................................................... 0.09g CuSO4·5H2O ............................................................................... 0.04g MnSO4 ........................................................................................ 0.02g Na2B4O7 ...................................................................................... 0.01g (NH4)6Mo7O24·4H2O ................................................................. 5.0mg © 2010 by Taylor and Francis Group, LLC

water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Combine solution I, 10.0mL of solution

Use: For the isolation and autotrophic cultivation of Thiobacillus intermedius.

Thiobacillus intermedius Medium Composition per 1010.0mL: Glucose ....................................................................................... 10.0g Na2S2O3·5H2O ............................................................................ 10.0g Solution I ......................................................................................1.0L Solution II ................................................................................10.0mL

Solution I: Composition per liter: NH4Cl ........................................................................................... 1.0g K2HPO4......................................................................................... 0.6g MgCl2·6H2O ................................................................................. 0.5g KH2PO4......................................................................................... 0.4g MgSO4 .......................................................................................... 0.3g CaCl2·2H2O .................................................................................. 0.2g FeCl3·6H2O................................................................................. 0.02g

Preparation of Solution I: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Solution II: Composition per liter: CaCl2·2H2O .................................................................................. 0.1g ZnSO4·7H2O ............................................................................... 0.09g CuSO4·5H2O............................................................................... 0.04g MnSO4 ........................................................................................ 0.02g Na2B4O7 ...................................................................................... 0.01g (NH4)6Mo7O24·4H2O ................................................................. 5.0mg

Preparation of Solution II: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Combine 1.0L of solution I, 10.0mL of solution II, 10.0g of glucose, and 10.0g of Na2S2O3·5H2O. Mix thoroughly. Filter sterilize. Aseptically distribute into sterile tubes or flasks.

Use: For the isolation and mixotrophic cultivation of Thiobacillus intermedius.

Thiobacillus intermedius Medium Composition per 1010.0mL: Glucose ....................................................................................... 10.0g Yeast extract.................................................................................. 0.3g Solution I ......................................................................................1.0L Solution II ................................................................................10.0mL

Solution I: Composition per liter: NH4Cl ........................................................................................... 1.0g K2HPO4......................................................................................... 0.6g MgCl2·6H2O ................................................................................. 0.5g KH2PO4......................................................................................... 0.4g MgSO4 .......................................................................................... 0.3g CaCl2·2H2O .................................................................................. 0.2g FeCl3·6H2O................................................................................. 0.02g

1760

Thiobacillus intermedius Medium

Preparation of Solution I: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Solution II: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Combine 1.0L of solution I, 10.0mL of solution II, 10.0g of glucose, and 0.3g of yeast extract. Mix thoroughly. Filter sterilize. Aseptically distribute into sterile tubes or flasks.

Use: For the isolation and heterotrophic cultivation of Thiobacillus intermedius.

Thiobacillus intermedius Medium (LMG 113) Composition per liter: Agar ............................................................................................ 15.0g Na2S2O3·5H2O ............................................................................ 10.0g MgCl2·H2O.................................................................................... 1.0g NH4Cl ........................................................................................... 1.0g Yeast extract.................................................................................. 1.0g K2HPO4 ......................................................................................... 0.6g KH2PO4 ......................................................................................... 0.4g Chlorophenol Red .................................................................... 80.0mg FeCl3·H2O ................................................................................ 33.0mg

Use: For the cultivation of Thiobacillus intermedius.

Thiobacillus Medium Composition per 100.0mL:

FeCl3·6H2O ................................................................................. 0.02g MnSO4·4H2O .............................................................................. 0.02g pH 6.6 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into flasks in 100.0mL volumes. Autoclave for 60 min at 0 psi pressure–100°C on 3 consecutive days.

Use: For the cultivation of nonaciduric Thiobacillus species.

Thiobacillus Medium Composition per liter: Na2S2O3·5H2O ............................................................................ 10.0g Na2HPO4·7H2O............................................................................. 7.9g Sodium formate ............................................................................ 6.8g Glucose ......................................................................................... 3.6g KNO3 ............................................................................................ 2.0g KH2PO4......................................................................................... 1.5g NH4Cl ........................................................................................... 0.3g MgSO4·7H2O ................................................................................ 0.1g Trace metals solution .................................................................5.0mL pH 7.6–8.5 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.6–8.5. Filter sterilize. Aseptically distribute into sterile tubes or flasks.

Na2S2O3·5H2O .............................................................................. 1.0g KH2PO4 ......................................................................................... 0.1g NH4Cl ........................................................................................... 0.1g MgCl2·7H2O................................................................................ 0.05g pH 6.8 ± 0.2 at 25°C

cies.

Preparation of Medium: Add components to distilled/deionized

Composition per liter:

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Thiobacillus thioparus and Thiobacillus thiooxidans.

Thiobacillus Medium Composition per liter: Na2S2O3·5H2O ............................................................................ 10.0g K2HPO4 ......................................................................................... 4.0g KH2PO4 ......................................................................................... 4.0g CaCl2 ............................................................................................. 0.1g MgSO4·7H2O ................................................................................ 0.1g (NH4)2SO4 ..................................................................................... 0.1g © 2010 by Taylor and Francis Group, LLC

Use: For the isolation and anaerobic cultivation of Thiobacillus spe-

Thiobacillus Medium Na2S2O3·5H2O ............................................................................ 10.0g Na2HPO4·7H2O............................................................................. 7.9g Sodium formate ............................................................................ 6.8g Glucose ......................................................................................... 3.6g KH2PO4......................................................................................... 1.5g NH4Cl ........................................................................................... 0.3g MgSO4·7H2O ................................................................................ 0.1g Trace metals solution .................................................................5.0mL pH 7.6–8.5 at 25°C

Thiobacillus Medium

CaCl2·2H2O................................................................................. 7.34g FeSO4·7H2O.................................................................................. 5.0g MnCl2·2H2O.................................................................................. 2.5g ZnSO4·7H2O ................................................................................. 2.2g CoCl2·6H2O .................................................................................. 0.5g (NH4)6Mo7O24·4H2O .................................................................... 0.5g CuSO4·5H2O ................................................................................. 0.2g

Use: For the isolation and aerobic cultivation of Thiobacillus species.

Thiobacillus Medium (ATCC Medium 64) Composition per 500.0mL:

1761

wetted. Autoclave for 30 min at 0 psi pressure–100°C on 3 consecutive days. Be sure that sulfur remains on the surface of the broth during the sterilization.

Use: For the cultivation and maintenance of a variety of Thiobacillus species.

Thiobacillus Medium (ATCC Medium 152) Composition per liter: Agar ............................................................................................ 15.0g Na2S2O3·5H2O ............................................................................ 10.0g NH4Cl ........................................................................................... 1.0g Yeast extract.................................................................................. 1.0g K2HPO4......................................................................................... 0.6g MgCl2............................................................................................ 0.5g KH2PO4......................................................................................... 0.4g Chlorophenol Red....................................................................... 0.08g FeCl3 ........................................................................................... 0.02g

Preparation of Medium: Add components to distilled/deionized

Solution A ..............................................................................400.0mL Solution B ..............................................................................100.0mL pH 2.8 ± 0.2 at 25°C

Solution A: Composition per 400.0mL:

Use: For the cultivation and maintenance of a variety of Thiobacillus species.

(NH4)2SO4 ..................................................................................... 0.4g KH2PO4 ......................................................................................... 0.2g MgSO4·7H2O .............................................................................. 0.08g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Solution B: Composition per 100.0mL: FeSO4·7H2O................................................................................ 10.0g H2SO4 (1N solution) ..................................................................1.0mL

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Preparation of Medium: Aseptically add 100.0mL of cooled sterile solution B to 400.0mL of cooled sterile solution A. Mix thoroughly. Adjust pH to 2.8. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of a variety of Thiobacillus species.

Thiobacillus Medium (ATCC Medium 125) Composition per liter: Sulfur .......................................................................................... 10.0g KH2PO4 ......................................................................................... 3.0g MgSO4·7H2O ................................................................................ 0.5g CaCl2 ........................................................................................... 0.25g (NH4)2SO4 ..................................................................................... 0.2g FeSO4·7H2O............................................................................... 5.0mg

Preparation of Medium: Add components, except sulfur, to tap water and bring volume to 1.0L. Mix thoroughly. Add 1.0g of sulfur to each of 10 flasks. Distribute the broth in 100.0mL volumes into the flasks. Pour the broth down the side of the flask so that the sulfur is not © 2010 by Taylor and Francis Group, LLC

Thiobacillus Medium (ATCC Medium 426) Composition per liter: Na2S2O3·5H2O ............................................................................ 10.0g Na2HPO4·7H2O............................................................................. 7.9g KH2PO4......................................................................................... 1.5g NH4Cl ........................................................................................... 0.3g MgSO4·7H2O ................................................................................ 0.1g Phenol Red................................................................................. 2.0mg Trace metals solution .................................................................5.0mL pH 8.5 ± 0.2 at 25°C

Trace Metals Solution: Composition per liter: EDTA .......................................................................................... 50.0g ZnSO4 ......................................................................................... 22.0g CaCl2 ........................................................................................... 5.54g MnCl2.......................................................................................... 5.06g FeSO4·7H2O................................................................................ 4.99g CoCl2 .......................................................................................... 1.61g CuSO4 ......................................................................................... 1.57g (NH4)2MoO4·4H2O ....................................................................... 1.1g

Preparation of Trace Metals Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0 with KOH.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.5 with 10% Na2CO3. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Adjust pH to 8.5 with sterile 10% Na2CO3 if necessary. The broth should be pink.

Use: For the cultivation and maintenance of a variety of Thiobacillus species.

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Thiobacillus Medium

Thiobacillus Medium (ATCC Medium 528) Composition per liter: Na2S2O3 ...................................................................................... 10.0g Yeast extract.................................................................................. 5.0g NH4Cl ........................................................................................... 1.0g K2HPO4 ......................................................................................... 0.6g MgCl2 ............................................................................................ 0.5g KH2PO4 ......................................................................................... 0.4g MgSO4 .......................................................................................... 0.3g Bromthymol Blue ....................................................................... 0.03g FeCl3 ........................................................................................... 0.02g Heavy metal solution ...............................................................30.0mL pH 6.8 ± 0.2 at 25°C

Heavy Metal Solution: Composition per liter: Ethylenediamine tetraacetate ........................................................ 1.5g FeSO4·7H2O.................................................................................. 0.2g ZnSO4·7H2O ................................................................................. 0.1g MnCl2·4H2O................................................................................ 0.02g Modified Hoagland trace elements solution ..............................6.0mL

Preparation of Heavy Metal Solution: Add EDTA to approximately 900.0mL of distilled/deionized water. Dissolve by adjusting pH to 7.0 with NaOH. Bring volume to 1.0L with distilled/deionized water.

Modified Hoagland Trace Elements Solution: Composition per 3.6L: H3BO3 ......................................................................................... 11.0g MnCl2·4H2O.................................................................................. 7.0g AlCl3 ............................................................................................. 1.0g CoCl2............................................................................................. 1.0g CuCl2............................................................................................. 1.0g KI .................................................................................................. 1.0g NiCl2 ............................................................................................. 1.0g ZnCl2 ............................................................................................. 1.0g BaCl2 ............................................................................................. 0.5g KBr................................................................................................ 0.5g LiCl ............................................................................................... 0.5g Na2MoO4....................................................................................... 0.5g SeCl4 ............................................................................................. 0.5g SnCl2·2H2O................................................................................... 0.5g NaVO3·H2O .................................................................................. 0.1g

Preparation of Modified Hoagland Trace Elements Solution: Prepare each component as a separate solution. Dissolve each

KH2PO4......................................................................................... 3.0g NH4Cl ........................................................................................... 0.1g MgCl2............................................................................................ 0.1g CaCl2 ............................................................................................. 0.1g pH 4.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Thiobacillus thiooxidans and Streptomyces scabies.

Thiobacillus neapolitanus Medium Composition per 1002.0mL: Solution I ......................................................................................1.0L Solution II ..................................................................................2.0mL pH 6.2–7.0 at 25°C

Solution I: Composition per liter: Na2S2O3·5H2O ............................................................................ 10.0g KH2PO4......................................................................................... 4.0g K2HPO4......................................................................................... 4.0g MgSO4·7H2O ................................................................................ 0.8g KHCO3.......................................................................................... 0.7g NH4Cl ........................................................................................... 0.4g

Preparation of Solution I: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Solution II: Composition per liter: Disodium EDTA ......................................................................... 50.0g NaOH.......................................................................................... 11.0g CaCl2·2H2O ................................................................................ 7.34g FeSO4·7H2O.................................................................................. 5.0g MnCl2·2H2O ................................................................................. 2.5g ZnSO4·7H2O ................................................................................. 2.2g CoCl2·6H2O .................................................................................. 0.5g (NH4)6Mo7O24·4H2O .................................................................... 0.5g CuSO4·5H2O ................................................................................. 0.2g

Preparation of Solution II: Add EDTA to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 6.0 with NaOH. Add remaining components, one by one. Maintain the pH at 6.0. After dissolution of all the salts, adjust the pH to 4.0 with HCl. Store at 4°C.

salt in approximately 100.0mL of distilled/deionized water. Adjust the pH of each solution to below 7.0. Combine all the salt solutions and bring the volume to 3.6L with distilled/deionized water. Adjust the pH to 3–4. A yellow precipitate may form after mixing. After a few days, it will turn into a fine white precipitate. Mix the solution thoroughly .

Preparation of Medium: Aseptically combine 1.0L of solution I and 2.0mL of solution II. Mix thoroughly. Adjust pH to 6.2–7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Preparation of Medium: Add components to distilled/deionized

Use: For the isolation and cultivation of Thiobacillus neapolitanus.

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of a variety of Thiobacillus species.

Thiobacillus Medium B Composition per liter: Noble agar................................................................................... 15.0g Na2S2O3·5H2O............................................................................... 5.0g © 2010 by Taylor and Francis Group, LLC

Thiobacillus novellus Medium Composition per liter: Na2S2O3·5H2O ............................................................................ 10.0g K2HPO .......................................................................................... 4.0g KH2PO4......................................................................................... 1.5g MgSO4·7H2O ................................................................................ 0.5g (NH4)2SO4 .................................................................................... 0.3g

Thiobacillus plumbophilus Medium

Yeast extract.................................................................................. 0.3g Trace metals solution ...............................................................10.0mL pH 6.8–7.2 at 25°C

Trace Metals Solution: Composition per liter: Disodium EDTA ......................................................................... 50.0g NaOH .......................................................................................... 11.0g CaCl2·2H2O................................................................................. 7.34g FeSO4·7H2O.................................................................................. 5.0g MnCl2·2H2O.................................................................................. 2.5g ZnSO4·7H2O ................................................................................. 2.2g CoCl2·6H2O .................................................................................. 0.5g (NH4)6Mo7O24·4H2O .................................................................... 0.5g CuSO4·5H2O ................................................................................. 0.2g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of Thiobacillus novellus.

Thiobacillus perometabolis Medium Composition per liter: Na2S2O3 ...................................................................................... 10.0g Yeast extract.................................................................................. 5.0g NH4Cl ........................................................................................... 1.0g K2HPO4 ......................................................................................... 0.6g MgCl2 ............................................................................................ 0.5g KH2PO4 ......................................................................................... 0.4g MgSO4 .......................................................................................... 0.3g Bromthymol Blue ....................................................................... 0.03g FeCl3 ........................................................................................... 0.02g Heavy metal solution ...............................................................30.0mL pH 3.0–4.0 at 25°C

Heavy Metal Solution: Composition per liter: Ethylenediamine tetraacetate ........................................................ 1.5g FeSO4·7H2O.................................................................................. 0.2g ZnSO4·H2O ................................................................................... 0.1g MnCl2·4H2O................................................................................ 0.02g Modified Hoagland trace elements solution ..............................6.0mL

Preparation of Heavy Metal Solution: Add ethylenediamine tetraacetate to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 6.8 to dissolve EDTA. Add remaining components. Bring volume to 1.0L with distilled/deionized water. Mix thoroughly. Adjust pH to 6.8. Modified Hoagland Trace Elements Solution: Composition per liter: H3BO3 ......................................................................................... 11.0g MnCl2·4H2O.................................................................................. 7.0g AlCl3 ............................................................................................. 1.0g CoCl2............................................................................................. 1.0g CuCl2............................................................................................. 1.0g Kl .................................................................................................. 1.0g NiCl2 ............................................................................................. 1.0g ZnCl2 ............................................................................................. 1.0g © 2010 by Taylor and Francis Group, LLC

1763

BaCl2 ............................................................................................. 0.5g KBr ............................................................................................... 0.5g LiCl ............................................................................................... 0.5g Na2MoO4 ...................................................................................... 0.5g SeS2 ............................................................................................... 0.5g SnCl2·2H2O................................................................................... 0.5g NaVO3·H2O .................................................................................. 0.1g

Preparation of Modified Hoagland Trace Elements Solution: Add components seqentially to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3–4. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. A flaky yellow precipitate forms after mixing but will change in a few days to a fine, white precipitate. Mix the medium thoroughly before use.

Use: For the cultivation of Thiobacillus perometabolis and other bacteria that can utilize thiosulfate as an energy source.

Thiobacillus plumbophilus Medium Composition per 1010.0mL: NiCl2·6H2O................................................................................... 5.0g MgSO4·7H2O .............................................................................. 3.45g MgCl2·6H2O ............................................................................... 2.75g NH4Cl ......................................................................................... 1.25g NaCl.............................................................................................. 0.5g KCl................................................................................................ 0.3g CaCl2·2H2O ................................................................................ 0.14g K2HPO4....................................................................................... 0.14g Na2WO4·2H2O ........................................................................... 0.5mg Trace elements solution ...........................................................10.0mL pH 6.0 ± 0.2 at 25°C

Trace Elements Solution: Composition per liter: MgSO4·7H2O ............................................................................... 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl.............................................................................................. 1.0g MnSO4·2H2O ............................................................................... 0.5g CoSO4·7H2O............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g KAI(SO4)2·12H2O ...................................................................... 0.02g CuSO4·5H2O............................................................................... 0.01g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O ......................................................................... 0.01g NiCl2·6H2O............................................................................... 0.025g Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0 with H2SO4. Distribute 20.0mL volumes into 100.0mL serum bottles. Sparge with 80% H2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Check pH of medium after autoclaving and bring to 6.0 if necessary. After inoculation add 3% sterile air (9.0mL per serum bottle) with a syringe. Pressurize bottles to 2 bar with 80% H2 + 20% CO2.

1764

Thiobacillus prosperus Medium

Use: For the cultivation and maintenance of Thiobacillus plumbophi-

Preparation of Phosphate Solution: Add components to dis-

lus.

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Thiobacillus prosperus Medium Composition per 1010.0mL: MgSO4·7H2O .............................................................................. 3.45g MgCl2·6H2O................................................................................ 2.75g NH4Cl ......................................................................................... 1.25g NaCl .............................................................................................. 0.5g Sulfur, powdered........................................................................... 0.5g KCl.............................................................................................. 0.33g CaCl2·2H2O................................................................................. 0.14g K2HPO4 ....................................................................................... 0.14g KH2PO4 ....................................................................................... 0.14g NiCl2·6H2O ................................................................................ 2.0mg Trace elements solution ...........................................................10.0mL pH 2.5 ± 0.2 at 25°C

Preparation of Sulfur: Add 10.0g of powdered sulfur to a flask and sterilize by steaming for 3 hr on 3 consecutive days. Trace Elements Solution: Composition per liter: MgSO4·7H2O ............................................................................... 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl .............................................................................................. 1.0g MnSO4·2H2O ............................................................................... 0.5g CoSO4·7H2O ............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O................................................................................... 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O ............................................................................... 0.025g KAI(SO4)2·12H2O....................................................................... 0.02g CuSO4·5H2O ............................................................................... 0.01g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O ......................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Trace Metals A-5: Composition per liter: H3BO3 ......................................................................................... 2.86g MnCl2·4H2O ............................................................................... 1.81g Na2MoO4·2H2O .......................................................................... 0.39g ZnSO4·7H2O ............................................................................. 0.222g CuSO4·5H2O ............................................................................. 0.079g Co(NO3)2·6H2O ....................................................................... 49.4mg

Preparation of Trace Metals A-5: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, except phosphate solution, to distilled/deionized water and bring volume to 900.0mL. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 100.0mL of the sterile phosphate solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Thiobacillus tepidarius.

Thiobacillus/Thermophilus Medium Composition per liter: Na2S2O3·5H2O .............................................................................. 5.0g NaHCO3 ........................................................................................ 1.0g Na2HPO4 ....................................................................................... 0.2g MgCl2............................................................................................ 0.1g NH4·Cl .......................................................................................... 0.1g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 20 min at 6 psi pressure–109°C or filter sterilize.

Use: For the cultivation of Thiobacillus species and Thermophilus

Preparation of Trace Elements Solution: Add nitrilotriacetic

species.

acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L.

Composition per liter:

Preparation of Medium: Add components, except sulfur, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 2.5 with H2SO4. Autoclave for 15 min at 15 psi pressure– 121°C. Aseptically add 0.5g of sterile sulfur. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Thiobacillus prosperus.

Thiobacillus tepidarius Medium Composition per liter: Agar ............................................................................................ 10.0g Na2S2O3·5H2O ............................................................................ 4.96g MgSO4·7H2O ................................................................................ 0.8g NH4Cl ........................................................................................... 0.4g Phosphate solution .................................................................100.0mL Bromcresol Purple, saturated solution .......................................2.0mL Trace metals A-5........................................................................1.0mL

Phosphate Solution: Composition per 100.0mL: KH2PO4 ......................................................................................... 4.0g K2HPO4 ......................................................................................... 4.0g © 2010 by Taylor and Francis Group, LLC

Thiobacillus thiooxidans Medium Sulfur, powdered......................................................................... 10.0g KH2PO4......................................................................................... 5.0g MgSO4·7H2O ................................................................................ 0.5g CaCl2 ........................................................................................... 0.25g (NH4)2SO4 .................................................................................... 0.2g FeSO4 .......................................................................................... 0.01g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components, except sulfur, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into flasks in 100.0mL volumes. Add 1.0g of sulfur to each flask. Autoclave for 30 min at 0 psi pressure–100°C on 3 consecutive days. Use: For the cultivation of Thiobacillus thiooxidans.

Thiobacillus thiooxidans Medium Composition per liter: Flowers of sulfur........................................................................... 5.0g K2HPO .......................................................................................... 3.5g MgSO4·7H2O ................................................................................ 0.5g (NH4)2SO4 .................................................................................... 0.3g

Thiobacillus thioparus Medium

CaCl2 ........................................................................................... 0.25g FeSO4·7H2O................................................................................ 0.02g pH 4.5 ± 0.2 at 25°C

Preparation of Medium: Add components, except flowers of sul-

1765

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.6. Distribute into tubes or flasks. Autoclave for 20 min at 10 psi pressure– 115°C.

fur, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into flasks or bottles in 100.0mL volumes. Add 0.5g of flowers of sulfur to each flask or bottle. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Thiobacillus thioparus.

Use: For the isolation and cultivation of Thiobacillus thiooxidans.

Na2S2O3·5H2O .............................................................................. 5.0g K2HPO4......................................................................................... 4.0g MgSO4·7H2O ................................................................................ 0.5g (NH4)2SO4 .................................................................................... 0.4g CaCl2 ........................................................................................... 0.25g FeSO4 .......................................................................................... 0.01g pH 7.0 ± 0.2 at 25°C

Thiobacillus thiooxidans Medium Composition per liter: Sulfur, powdered......................................................................... 10.0g KH2PO4 ......................................................................................... 3.0g CaCl2·2H2O................................................................................. 0.14g MgCl2·6H2O.................................................................................. 0.1g NH4Cl ........................................................................................... 0.1g pH 4.2 ± 0.2 at 25°C

Preparation of Sulfur: Add 10.0g of powdered sulfur to a flask and sterilize by autoclaving for 15 min at 8 psi pressure–112°C. Preparation of Medium: Add components, except sulfur, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 2.5 with H2SO4. Autoclave for 15 min at 15 psi pressure– 121°C. Aseptically layer 0.5g of sterile sulfur onto the surface of the medium.

Use: For the cultivation and maintenance of Thiobacillus species.

Thiobacillus thioparus Agar Composition per liter: Agar, purified .............................................................................. 12.0g Na2S2O3·5H2O ........................................................................... 10.0g K2HPO4 ......................................................................................... 4.0g KH2PO4 ......................................................................................... 4.0g MgSO4·7H2O ................................................................................ 0.1g (NH4)2SO4 ..................................................................................... 0.1g CaCl2 ............................................................................................. 0.1g FeCl3·6H2O ................................................................................. 0.02g MnSO4·H2O ................................................................................ 0.02g pH 6.6 ± 0.2 at 25°C

Preparation of Medium: Add components, except agar, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.6. Add agar. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 20 min at 10 psi pressure–115°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Thiobacillus thioparus.

Thiobacillus thioparus Broth

Thiobacillus thioparus Medium Composition per liter:

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Thiobacillus thioparus.

Thiobacillus thioparus II Medium (DSMZ Medium 486) Composition per 1003.0mL: Solution A..............................................................................900.0mL Solution B ..............................................................................100.0mL Vitamin solution.........................................................................3.0mL pH 7.4 ± 0.2 at 25°C Solution A: Composition per 900.0mL: Na2S2O3·5H2O .............................................................................. 5.0g NH4Cl ........................................................................................... 0.4g Na2CO3 ......................................................................................... 0.4g MgCl2·6H2O ................................................................................. 0.2g Bromcresol Purple (saturated solution) .....................................2.0mL Trace metals solution .................................................................1.0mL

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Solution B: Composition per 100.0mL: KH2PO4......................................................................................... 2.0g K2HPO4......................................................................................... 2.0g

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 7.6. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Composition per liter:

Trace Elements Solution: Composition per liter:

Na2S2O3·5H2O ........................................................................... 10.0g K2HPO4 ......................................................................................... 4.0g KH2PO4 ......................................................................................... 4.0g MgSO4·7H2O ................................................................................ 0.1g (NH4)2SO4 ..................................................................................... 0.1g CaCl2 ............................................................................................. 0.1g FeCl3·6H2O ................................................................................. 0.02g MnSO4·H2O ................................................................................ 0.02g pH 6.6 ± 0.2 at 25°C

Na2-EDTA................................................................................... 50.0g NaOH.......................................................................................... 11.0g ZnSO4·7H2O ............................................................................... 11.0g CaCl2·2H2O ................................................................................ 7.34g FeSO4·7H2O.................................................................................. 5.0g MnCl2·4H2O ................................................................................. 2.5g CoCl2·6H2O .................................................................................. 0.5g (NH4)6Mo7O24·4H2O .................................................................... 0.5g CuSO4·5H2O................................................................................. 0.2g

1766

Thiobacillus thioparusii Agar

Preparation of Trace Elements Solution: Add components to

Preparation of Solution B: Add components to distilled/deionized

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Vitamin Solution: Composition per liter: Riboflavin ................................................................................ 20.0mg Ca-pantothenate ....................................................................... 20.0mg Nicotinic acid ........................................................................... 20.0mg Pyridoxine-HCl ........................................................................ 20.0mg p-Aminobenzoic acid ............................................................... 10.0mg Thiamine-HCl·2H2O ................................................................ 10.0mg Biotin ......................................................................................... 1.0mg Vitamin B12 ................................................................................ 1.0mg

Use: For the cultivation and maintenance of Thiobacillus thioparus.

Thiobacillus thioparusii Agar

Vitamin Solution: Composition per liter: Calcium DL-pantothenate......................................................... 20.0mg Nicotinic acid........................................................................... 20.0mg Pyridoxine·HCl ........................................................................ 20.0mg Riboflavin ................................................................................ 20.0mg p-Aminobenzoic acid............................................................... 10.0mg Thiamine·HCl .......................................................................... 10.0mg Biotin ......................................................................................... 1.0mg Vitamin B12 ................................................................................ 1.0mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Filter sterilize.

Preparation of Medium: Aseptically combine 100.0mL of sterile solution A with 900.0mL of sterile solution B and 3.0mL of sterile vitamin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation and maintenance of Thiobacillus thioparus.

Composition per 1003.0mL: Solution B ..............................................................................900.0mL Solution A ..............................................................................100.0mL Vitamin solution.........................................................................3.0mL pH 7.1 ± 0.2 at 25°C

Solution A: Composition per 100.0mL: K2HPO4 ......................................................................................... 2.0g KH2PO4 ......................................................................................... 2.0g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Solution B: Composition per 900.0mL: Agar ............................................................................................ 15.0g Na2S2O3·5H2O .............................................................................. 5.0g Na2CO3 ......................................................................................... 0.4g NH4Cl ........................................................................................... 0.4g MgCl2·6H2O.................................................................................. 0.2g Bromcresol Purple (saturated aqueous solution) .......................2.0mL Trace elements solution .............................................................1.0mL

Trace Elements Solution: Composition per liter:

Thiobacillus thioparusii Broth Composition per 1003.0mL: Solution B ..............................................................................900.0mL Solution A..............................................................................100.0mL Vitamin solution.........................................................................3.0mL pH 7.1 ± 0.2 at 25°C

Solution A: Composition per 100.0mL: K2HPO4......................................................................................... 2.0g KH2PO4......................................................................................... 2.0g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Solution B: Composition per 900.0mL: Na2S2O3·5H2O .............................................................................. 5.0g Na2CO3 ......................................................................................... 0.4g NH4Cl ........................................................................................... 0.4g MgCl2·6H2O ................................................................................. 0.2g Bromcresol Purple (saturated aqueous solution) .......................2.0mL Trace elements solution .............................................................1.0mL

Trace Elements Solution: Composition per liter:

Disodium EDTA ......................................................................... 50.0g NaOH .......................................................................................... 11.0g ZnSO4·7H2O ............................................................................... 11.0g CaCl2·2H2O................................................................................. 7.34g FeSO4·7H2O.................................................................................. 5.0g MnCl2·4H2O.................................................................................. 2.5g CoCl2·6H2O .................................................................................. 0.5g (NH4)6MoO24·4H2O...................................................................... 0.5g CuSO4·5H2O ................................................................................. 0.2g

Disodium EDTA ......................................................................... 50.0g NaOH.......................................................................................... 11.0g ZnSO4·7H2O ............................................................................... 11.0g CaCl2·2H2O ................................................................................ 7.34g FeSO4·7H2O.................................................................................. 5.0g MnCl2·4H2O ................................................................................. 2.5g CoCl2·6H2O .................................................................................. 0.5g (NH4)6MoO24·4H2O...................................................................... 0.5g CuSO4·5H2O ................................................................................. 0.2g

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0.

Thiobacillus thyasiris Broth Preparation of Solution B: Add components to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Vitamin Solution: Composition per liter: Calcium DL-pantothenate......................................................... 20.0mg Nicotinic acid ........................................................................... 20.0mg Pyridoxine·HCl ........................................................................ 20.0mg Riboflavin ................................................................................ 20.0mg p-Aminobenzoic acid............................................................... 10.0mg Thiamine·HCl .......................................................................... 10.0mg Biotin ......................................................................................... 1.0mg Vitamin B12 ................................................................................ 1.0mg

1767

Vitamin Solution: Composition per liter:

deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Filter sterilize.

Calcium DL-pantothenate......................................................... 20.0mg Nicotinic acid........................................................................... 20.0mg Pyridoxine·HCl ........................................................................ 20.0mg Riboflavin ................................................................................ 20.0mg p-Aminobenzoic acid............................................................... 10.0mg Thiamine·HCl .......................................................................... 10.0mg Biotin ......................................................................................... 1.0mg Vitamin B12 ................................................................................ 1.0mg

Preparation of Medium: Aseptically combine 100.0mL of sterile

Preparation of Vitamin Solution: Add components to distilled/

solution A with 900.0mL of sterile solution B and 3.0mL of sterile vitamin solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Thiobacillus thioparus.

Thiobacillus thyasiris Agar Composition per 1010.0mL: Solution A ..............................................................................100.0mL Solution B ..............................................................................900.0mL Vitamin solution.......................................................................10.0mL pH 7.3–7.6 at 25°C

Solution B: Composition per 900.0mL: NaCl ............................................................................................ 25.1g Agar ............................................................................................ 15.0g Na2S2O3 ........................................................................................ 5.0g NH4Cl ........................................................................................... 0.4g MgSO4·7H2O ................................................................................ 0.1g Phenol Red ................................................................................. 3.0mg Trace elements solution ...........................................................10.0mL

Trace Elements Solution: Composition per liter: Disodium EDTA ......................................................................... 50.0g NaOH ......................................................................................... 11.0g ZnSO4·7H2O ............................................................................... 11.0g CaCl2·2H2O................................................................................. 7.34g FeSO4·7H2O.................................................................................. 5.0g MnCl2·4H2O.................................................................................. 2.5g CoCl2·6H2O .................................................................................. 0.5g (NH4)6MoO24·4H2O ..................................................................... 0.5g CuSO4·5H2O ................................................................................. 0.2g © 2010 by Taylor and Francis Group, LLC

deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Filter sterilize.

Preparation of Medium: Aseptically combine 100.0mL of sterile solution A with 900.0mL of sterile solution B and 10.0mL of sterile vitamin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation and maintenance of Thiobacillus thyasiris.

Thiobacillus thyasiris Broth Composition per 1010.0mL: Solution B ..............................................................................900.0mL Solution A..............................................................................100.0mL Vitamin solution.......................................................................10.0mL pH 7.3–7.6 at 25°C

Solution A: Composition per 100.0mL: Na2HPO42H2O.............................................................................. 7.9g KH2PO4......................................................................................... 1.5g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 7.4. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Solution B: Composition per 900.0mL: NaCl............................................................................................ 25.1g Na2S2O3 ........................................................................................ 5.0g NH4Cl ........................................................................................... 0.4g MgSO4·7H2O ................................................................................ 0.1g Phenol Red................................................................................. 3.0mg Trace elements solution ...........................................................10.0mL

1768

Thiobacillus thyasiris/Thiobacillus halophilus Medium

(NH4)6MoO24·4H2O...................................................................... 0.5g CuSO4·5H2O ................................................................................. 0.2g

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0. Preparation of Solution B: Add components to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Vitamin Solution: Composition per liter: Calcium DL-pantothenate......................................................... 20.0mg Nicotinic acid ........................................................................... 20.0mg Pyridoxine·HCl ........................................................................ 20.0mg Riboflavin ................................................................................ 20.0mg p-Aminobenzoic acid ............................................................... 10.0mg Thiamine·HCl .......................................................................... 10.0mg Biotin ......................................................................................... 1.0mg Vitamin B12 ................................................................................ 1.0mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Filter sterilize.

Preparation of Medium: Aseptically combine 100.0mL of sterile solution A with 900.0mL of sterile solution B and 10.0mL of sterile vitamin solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Thiobacillus thyasiris.

Thiobacillus thyasiris/Thiobacillus halophilus Medium (DSMZ Medium 484) Composition per 1010.0mL: Solution B ..............................................................................900.0mL Solution A ..............................................................................100.0mL Vitamin solution.......................................................................10.0mL pH 7.3–7.6 at 25°C Solution A: Composition per 100.0mL: Na2HPO4·2H2O............................................................................. 7.9g KH2PO4 ......................................................................................... 1.5g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 7.6. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Solution B: Composition per 900.0mL: NaCl ............................................................................................ 25.1g Na2S2O3 ........................................................................................ 5.0g NH4Cl ........................................................................................... 0.4g MgSO4·7H2O ................................................................................ 0.1g Phenol Red ................................................................................. 3.0mg Trace elements solution ...........................................................10.0mL

Preparation of Solution B: Add components to distilled/deionized

ZnSO4·7H2O ............................................................................... 11.0g CaCl2·2H2O ................................................................................ 7.34g FeSO4·7H2O.................................................................................. 5.0g MnCl2·4H2O ................................................................................. 2.5g CoCl2·6H2O .................................................................................. 0.5g (NH4)6Mo7O24·4H2O .................................................................... 0.5g CuSO4·5H2O ................................................................................. 0.2g

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Vitamin Solution: Composition per liter: Riboflavin ................................................................................ 20.0mg Ca-pantothenate ....................................................................... 20.0mg Nicotinic acid........................................................................... 20.0mg Pyridoxine-HCl........................................................................ 20.0mg p-Aminobenzoic acid............................................................... 10.0mg Thiamine-HCl·2H2O................................................................ 10.0mg Biotin ......................................................................................... 1.0mg Vitamin B12 ................................................................................ 1.0mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Preparation of Medium: Aseptically combine 100.0mL solution A, 900.0mL solution B, and 10.0mL vitamin solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation and maintenance of Thiobacillus thyasiris, Thiobacillus halophilus, Thiomicrospira thyasirae, and Halothiobacillus halophilus DSM6132.

Thiocapsa halophila Medium Composition per 1061.0mL: NaCl............................................................................................ 70.0g MgCl2·6H2O ................................................................................. 4.5g MgSO4·7H2O ................................................................................ 3.0g NH4Cl ........................................................................................... 0.5g KH2PO4......................................................................................... 0.3g CaCl2·2H2O ................................................................................ 0.05g NaHCO3 solution .....................................................................40.0mL Na2S·9H2O solution .................................................................10.0mL Na2S2O3 solution .....................................................................10.0mL Vitamin B12 solution ..................................................................1.0mL Trace elements solution SL-12B................................................1.0mL pH 7.2 ± 0.2 at 25°C

NaHCO3 Solution: Composition per 100.0mL: NaHCO3 ........................................................................................ 5.0g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Na2S·9H2O Solution: Composition per 10.0mL:

Trace Elements Solution: Composition per liter:

Na2S·9H2O.................................................................................... 0.6g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Thiocapsa Medium

Na2S2O3 Solution : Composition per 10.0mL: Na2S2O3 ·5H2O.............................................................................. 0.5g

Preparation of Na2S2O3 Solution: Add Na2S2O3·5H2O to dis-

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Vitamin B12 Solution: Composition per 10.0mL: Vitamin B12 ................................................................................ 0.2mg

Preparation of Vitamin B12 Solution: Add vitamin B12 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Trace Elements Solution SL-12B: Composition per liter: MnCl2·2H2O................................................................................ 50.0g Disodium EDTA ........................................................................... 3.0g FeSO4·7H2O................................................................................. 1.1g H3BO3 ........................................................................................... 0.3g CoCl2·6H2O ................................................................................ 0.19g ZnCl2 ........................................................................................ 42.0mg NiCl2·6H2O .............................................................................. 24.0mg Na2MoO4·2H2O ...................................................................... 18.0mg CuCl2·2H2O ............................................................................... 2.0mg

Preparation of Trace Elements Solution SL-12B: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, except NaHCO3 solu-

tion, Na2S·9H2O solution, Na2S2O3 solution, and vitamin B12 solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature while sparging with 90% N2 + 10% CO2. Aseptically and anaerobically add 40.0mL of sterile NaHCO3 solution, 10.0mL of sterile Na2S·9H2O solution, 10.0mL of sterile Na2S2O3 solution, and 1.0mL of sterile vitamin B12 solution. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Thiocapsa halophila.

Thiocapsa Medium Composition per 127.0mL: Solution 1.................................................................................76.2mL Solution 2 + Solution 3 ............................................................44.8mL Solution 4...................................................................................6.0mL

Solution 1: Composition per 2.5L: NaCl .......................................................................................... 39.68g CaCl2 ............................................................................................. 2.0g

Preparation of Solution 1: Add components to distilled/deionized water and bring volume to 2.5L. Distribute in 80.0mL volumes into 127.0mL screw-capped bottles. Autoclave for 15 min at 15 psi pressure–121°C. Solution 2: Composition per 100.0mL: Sodium ascorbate .......................................................................... 2.4g KC1............................................................................................... 1.0g KH2PO4 ......................................................................................... 1.0g MgCl2·6H2O.................................................................................. 0.8g NH4Cl ........................................................................................... 0.8g © 2010 by Taylor and Francis Group, LLC

1769

Heavy metal solution ...............................................................50.0mL Vitamin solution.......................................................................15.0mL Vitamin B12 solution ..................................................................3.0mL

Preparation of Solution 2: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Heavy Metal Solution: Composition per liter: Ethylenediamine tetraacetate (EDTA) .......................................... 1.5g FeSO4·7H2O.................................................................................. 0.2g ZnSO4·7H2O ................................................................................. 0.1g MnCl2·4H2O ............................................................................... 0.02g Modified Hoagland trace elements solution ..............................6.0mL

Preparation of Heavy Metal Solution: Dissolve EDTA in approximately 800.0mL of distilled/deionized water. Add remaining components. Bring volume to 1.0L with distilled/deionized water. Mix thoroughly.

Modified Hoagland Trace Elements Solution: Composition per 3.6L: H3BO3 ......................................................................................... 11.0g MnCl2·4H2O ................................................................................. 7.0g AlCl3 ............................................................................................. 1.0g CoCl2 ............................................................................................ 1.0g CuCl2 ............................................................................................ 1.0g KI .................................................................................................. 1.0g NiCl2 ............................................................................................. 1.0g ZnCl2 ............................................................................................. 1.0g BaCl2 ............................................................................................. 0.5g KBr ............................................................................................... 0.5g LiCl ............................................................................................... 0.5g Na2MoO4 ...................................................................................... 0.5g SeCl4 ............................................................................................. 0.5g SnCl2·2H2O................................................................................... 0.5g NaVO3·H2O .................................................................................. 0.1g

Preparation of Modified Hoagland Trace Elements Solution: Prepare each component as a separate solution. Dissolve each salt in approximately 100.0mL of distilled/deionized water. Adjust the pH of each solution to below 7.0. Combine all the salt solutions and bring the volume to 3.6L with distilled/deionized water. Adjust the pH to 3–4. A yellow precipitate may form after mixing. After a few days, it will turn into a fine white precipitate. Mix the solution thoroughly before using.

Vitamin Solution: Composition per 100.0mL: Pyridoxamine·2HCl ................................................................... 5.0mg Nicotinic acid............................................................................. 2.0mg Thiamine .................................................................................... 1.0mg Pantothenic acid......................................................................... 0.5mg Biotin ......................................................................................... 0.2mg p-Aminobenzoic acid................................................................. 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 100.0mL. Mix thoroughly. Vitamin B12 Solution: Composition per 100.0mL: Vitamin B12 (cyanocobalamin) .................................................. 2.0mg

Preparation of Vitamin B12 Solution: Add vitamin B12 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

1770

Thiocyanate Agar

NaHCO3 ........................................................................................ 4.5g

Preparation of Solution C: Add disodium succinate to distilled/ deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Preparation of Solution 3: Add NaHCO3 to distilled/deionized

Preparation of Medium: Aseptically combine 800.0mL of solu-

Solution 3: Composition per 900.0mL:

water and bring volume to 900.0mL. Mix thoroughly. Bubble 100% CO2 through the solution for 30 min. After CO2 saturation of solution 3, add solution 2 and immediately filter the mixture through a Seitz filter (or a Millipore) using positive CO2 pressure to push the liquid through.

Solution 4: Composition per 200.0mL: Na2S·9H2O .................................................................................... 3.0g

Preparation of Solution 4: Add Na2S·9H2O to distilled/deionized

water and bring volume to 200.0mL. Add a magnetic stir bar to the flask. Autoclave for 15 min at 15 psi pressure–121°C. On a magnetic stirrer, slowly add 2.0mL of sterile 2M H2SO4. This partially neutralizes the solution. The solution should turn yellow. H2S gas will be liberated; neutralization and distribution of the solution should be done as rapidly as possible under adequate ventilation.

Preparation of Medium: To the 80.0mL of sterile solution 1 in screw-capped bottles, add combined solutions 2 and 3 immediately after filtration and fill bottles to capacity. Mix thoroughly. Aseptically remove 6.0mL of the medium from the bottles and replace it with 6.0mL of neutralized solution 4. Let stand for 24 hr. The medium should form a fine white precipitate before using. To inoculate, remove 6.0mL of the completed medium from the bottles and replace it with 6.0mL of inoculum.

Use: For the cultivation and maintenance of a variety of Thiocapsa species.

tion A with 100.0mL of solution B and 100.0mL of solution C. Mix thoroughly. Pour into sterile Petri dishes or aseptically distribute into sterile tubes.

Use: For the cultivation and maintenance of a variety of microorganisms that can utilize thiocyanante as sole source of nitrogen and sulfur.

Thiocyanate Utilization Medium Composition per 1225.0mL: Basal solution................................................................................1.0L Solution C ..............................................................................200.0mL Solution B ................................................................................20.0mL Solution A..................................................................................5.0mL

Basal Solution: Composition per liter: Na2HPO4 ....................................................................................... 4.8g KH2PO4......................................................................................... 4.4g MgSO4·7H2O ................................................................................ 0.5g

Preparation of Basal Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Solution A: Composition per 100.0mL: FeCl3·6H2O ................................................................................... 1.0g CaCl2 ............................................................................................. 0.1g

Preparation of Solution A: Add components to distilled/deionized

Thiocyanate Agar Composition per liter: Solution A ..............................................................................800.0mL Solution B ..............................................................................100.0mL Solution C ..............................................................................100.0mL

Solution A: Composition per 800.0mL: Agar, noble.................................................................................. 30.0g K2HPO4 ......................................................................................... 1.0g KH2PO4 ......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.2g CaCl2 ........................................................................................ 20.0mg FeCl3·6H2O (60%) .....................................................................0.1mL

Solution B: Composition per 100.0mL: KCNS............................................................................................ 3.6g

Preparation of Solution B: Add KCNS to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Solution B: Composition per 100.0mL: D-Glucose .................................................................................... 10.0g

Preparation of Solution B: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Solution C: Composition per 200.0mL: NaSCN.......................................................................................... 1.0g

Preparation of Solution C: Add NaSCN to distilled/deionized water and bring volume to 200.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: To 1.0L of cooled, sterile basal solution, aseptically add 5.0mL of sterile solution A, 20.0mL of sterile solution B, and 200.0mL of sterile solution C. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of a variety of microorganisms that can utilize thiocyanate as sole source of nitrogen and sulfur.

Thiofaba Medium (DSMZ Medium 1114) Composition per liter: Solution A..............................................................................960.0mL Solution B ................................................................................10.0mL Solution C ................................................................................10.0mL Solution D................................................................................10.0mL Solution E ................................................................................10.0mL pH 6.5 ± 0.2 at 25°C

Thioglycolate Bile Broth

Solution A: Composition per 960.0mL: MgCl2·6H2O................................................................................ 0.75g CaCl2·2H2O................................................................................. 0.15g NH4Cl ........................................................................................ 0.54g Trace elements solution .............................................................2.0mL

Trace Elements Solution: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl .............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoSO4·7H2O ............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O................................................................................... 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O ............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O ............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Mix thoroughly. Preparation of Solution A: Add components to distilled/deionized water and bring volume to 960.0mL. Mix thoroughly. Sparge with a gas mixture of 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Solution B: Composition per 10.0mL: KH2PO4 ....................................................................................... 1.19g KH2PO4 ....................................................................................... 0.21g

1771

Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Solution E: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Preparation of Medium: Dispense solution A under a gas atmosphere of 80% N2 + 20% CO2 into serum vials to 20% of volume. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aspetically add sterile solutions B–E in that order. Adjust the pH to 6.5. After inoculation pressurize with an amount of sterile air that is equivalent to 30% of the volume of the cultivation vessel.

Use: For the cultivation of Thiofaba spp.

Thiogel® Medium Composition per liter: Gelatin......................................................................................... 50.0g Pancreatic digest of casein.......................................................... 17.0g Glucose ......................................................................................... 6.0g Papaic digest of soybean meal...................................................... 3.0g NaCl.............................................................................................. 2.5g Sodium thioglycolate .................................................................... 0.5g Agar .............................................................................................. 0.7g Na2SO3 .......................................................................................... 0.1g L-Cystine ..................................................................................... 0.25g pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium: Add components to distilled/deionized water preheated to 50°C and bring volume to 1.0L. Mix thoroughly. Let stand for 5 min. Gently heat while stirring and bring to boiling. Distribute into tubes, filling them half full. Autoclave for 15 min at 13 psi pressure–118°C. Pour into sterile Petri dishes or leave in tubes.

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave or 15 min at 15 psi pressure–121°C. Cool to room temperature.

Use: For the differentiation of microorganisms based on their ability

Solution C: Composition per 10.0mL:

Composition per 1050.0mL:

Na2CO3 ......................................................................................... 4.0g

Preparation of Solution C: Add Na2CO3 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with a gas mixture of 80% N2 + 20% CO2. Filter sterilize.

Solution D: Composition per 10.0mL: Na2S2O3·5H2O ............................................................................ 1.25g

Preparation of Solution D: Add Na2S2O3·5H2O to distilled/deion-

ized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Solution E: Composition per liter: Pyridoxine-HCl ........................................................................ 10.0mg Thiamine-HCl·2H2O .................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg D-Ca-pantothenate...................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg © 2010 by Taylor and Francis Group, LLC

to liquefy gelatin.

Thioglycolate Bile Broth Pancreatic digest of casein.......................................................... 15.0g Glucose ......................................................................................... 5.5g Yeast extract.................................................................................. 5.0g NaCl.............................................................................................. 2.5g Agar ............................................................................................ 0.75g L-Cystine ....................................................................................... 0.5g Sodium thioglycolate .................................................................... 0.5g Bile solution.............................................................................50.0mL pH 7.1 ± 0.2 at 25°C

Bile Solution: Composition per 100.0mL: Oxgall ......................................................................................... 40.0g Sodium deoxycholate.................................................................... 2.0g

Preparation of Bile Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except bile solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 10.0mL vol-

1772

Thioglycolate Broth USP, Alternative

umes. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°– 50°C. Aseptically add 0.5mL of sterile bile solution to each tube. Mix thoroughly.

Source: This medium is available as a premixed powder from Hi-

Use: For the cultivation of Bacteroides fragilis and Clostridium per-

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Prepare freshly or boil and cool the medium just before use. Pour into sterile Petri dishes or distribute into sterile tubes.

fringens from clinical specimens.

Thioglycolate Broth USP, Alternative

Media.

Preparation of Medium: Add components to distilled/deionized

Composition per liter:

Use: For the cultivation of facultative and anaerobic organisms. For

Pancreatic digest of casein .......................................................... 15.0g Glucose ......................................................................................... 5.5g Yeast extract.................................................................................. 5.0g NaCl .............................................................................................. 2.5g L-Cystine ....................................................................................... 0.5g Sodium thioglycolate .................................................................... 0.5g pH 7.1 ± 0.2 at 25°C

Composition per liter:

Source: This medium is available as a premixed powder from Oxoid Unipath.

the performance of sterility tests of turbid or viscous specimens.

Thioglycolate HiVeg Medium without Indicator Plant hydrolysate ........................................................................ 17.0g Glucose ......................................................................................... 6.0g Papaic digest of soybean meal...................................................... 3.0g NaCl.............................................................................................. 2.5g Agar .............................................................................................. 0.7g Na-thioglycolate ........................................................................... 0.5g L-Cystine..................................................................................... 0.25g Na2SO3 ......................................................................................... 0.1g pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-

Use: For the cultivation of both aerobic and anaerobic organisms in

Media.

the performance of sterility tests of turbid or viscous specimens.

Preparation of Medium: Add components to distilled/deionized

Thioglycolate Gelatin Medium Composition per liter: Gelatin......................................................................................... 50.0g Pancreatic digest of casein .......................................................... 15.0g Yeast extract.................................................................................. 5.0g NaCl .............................................................................................. 2.5g Glucose ......................................................................................... 2.0g Agar ............................................................................................ 0.75g L-Cystine ..................................................................................... 0.25g Na2SO3 .......................................................................................... 0.1g Thioglycollic acid ......................................................................0.3mL pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to 50°C. Let stand 5 min. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the determination of gelatin liquefaction by aerobes, microaerophiles, and anaerobes without special incubation.

Thioglycolate HiVeg Agar Composition per liter: Agar ............................................................................................ 20.0g Plant hydrolysate......................................................................... 15.0g Glucose ......................................................................................... 5.5g Yeast extract.................................................................................. 5.0g NaCl .............................................................................................. 2.5g L-Cystine ....................................................................................... 0.5g Na-thioglycolate............................................................................ 0.5g Resazurin ................................................................................... 1.0mg pH 7.1 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Prepare freshly or boil and cool the medium just before use.

Use: For the cultivation of facultative and anaerobic organisms. For the performance of sterility tests of turbid or viscous specimens.

Thioglycolate Medium (DSMZ Medium 530) Composition per liter: Agar ............................................................................................ 15.0g Peptone from casein...................................................................... 5.0g Meat extract .................................................................................. 3.0g Na-thioglycolate solution.......................................................100.0mL pH 5.5 ± 0.2 at 25°C

Thioglycolate Solution : Composition per 100.0mL: Na-thioglycolate ........................................................................... 0.5g

Preparation of Thioglycolate Solution: Add thioglycolate to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Warm to 50°C.

Preparation of Medium: Add components, except thioglycolate solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 100.0mL warm thioglycolate solution. Adjust pH to 5.5. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation and maintenance of various anaerobic bacteria.

Thioglycolate Medium with 20% Bile (THIO + Bile Medium) Composition per liter: Oxgall ......................................................................................... 20.0g Thioglycolate medium without indicator......................................1.0L

Thioglycolate Medium, Enriched

Hemin solution...........................................................................0.5mL Vitamin K1 solution ...................................................................0.1mL pH 7.0 ± 0.2 at 25°C

Thioglycolate Medium without Indicator: Composition per liter: Pancreatic digest of casein .......................................................... 17.0g Glucose ......................................................................................... 6.0g Papaic digest of soybean meal ...................................................... 3.0g NaCl .............................................................................................. 2.5g Agar .............................................................................................. 0.7g Sodium thioglycolate .................................................................... 0.5g L-Cystine ..................................................................................... 0.25g Na2SO3 .......................................................................................... 0.1g

Preparation of Thioglycolate Medium Without Indicator:

1773

Thioglycolate Medium, Brewer Modified Composition per liter: Pancreatic digest of casein.......................................................... 17.5g Glucose ....................................................................................... 10.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 2.5g K2HPO4......................................................................................... 2.0g Sodium thioglycolate .................................................................... 1.0g Agar .............................................................................................. 0.5g Methylene Blue......................................................................... 0.002g pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium: Add components to distilled/deionized

Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks, filling them half full. Autoclave for 15 min at 15 psi pressure–121°C.

Vitamin K1 Solution: Composition per 100.0mL:

Use: For the cultivation of obligate anaerobes, microaerophiles, and facultative organisms.

Vitamin K1 .................................................................................... 1.0g

Preparation of Vitamin K1 Solution: Add vitamin K1 to 99.0mL of absolute ethanol. Mix thoroughly.

Hemin Solution: Composition per 100.0mL: Hemin............................................................................................ 1.0g NaOH (1N solution).................................................................20.0mL

Preparation of Hemin Solution: Add hemin to 20.0mL of 1N NaOH solution. Mix thoroughly. Bring volume to 100.0mL with distilled/deionized water.

Preparation of Medium: Add 0.5mL of hemin solution, 0.1mL of vitamin K1 solution, and oxgall to 1.0L of thioglycolate medium without indicator. Mix thoroughly. Distribute into screw-capped tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool tubes or flasks under 85% N2 + 10% H2 + 5% CO2. Tighten caps.

Use: For the isolation, cultivation, and identification of a variety of obligate anaerobic bile-tolerant bacteria.

Thioglycolate Medium, Brewer Composition per liter: Glucose ......................................................................................... 5.0g Peptone.......................................................................................... 5.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 2.0g Sodium thioglycolate .................................................................... 1.1g Agar .............................................................................................. 1.0g Beef extract ................................................................................... 1.0g Methylene Blue.......................................................................... 2.0mg pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath.

Preparation of Medium: Add components to distilled/deionized

Thioglycolate Medium, Enriched (THIO Medium) (Thioglycolate Medium with Vitamin K1 and Hemin)

Composition per liter:

Thioglycolate medium without indicator......................................1.0L Hemin solution...........................................................................0.5mL Vitamin K1 solution ...................................................................0.1mL pH 7.0 ± 0.2 at 25°C

Thioglycolate Medium without Indicator: Composition per liter: Pancreatic digest of casein.......................................................... 17.0g Glucose ......................................................................................... 6.0g Papaic digest of soybean meal...................................................... 3.0g NaCl.............................................................................................. 2.5g Agar .............................................................................................. 0.7g Sodium thioglycolate .................................................................... 0.5g L-Cystine ..................................................................................... 0.25g Na2SO3 .......................................................................................... 0.1g

Source: Thioglycolate medium without indicator is available as a premixed powder from Oxoid Unipath and BD Diagnostic Systems.

Preparation of Thioglycolate Medium without Indicator: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Vitamin K1 Solution: Composition per 100.0mL: Vitamin K1 .................................................................................... 1.0g

Preparation of Vitamin K1 Solution: Add vitamin K1 to 99.0mL of absolute ethanol. Mix thoroughly.

Hemin Solution: Composition per 100.0mL: Hemin ........................................................................................... 1.0g NaOH (1N solution).................................................................20.0mL

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Hemin Solution: Add hemin to 20.0mL of 1N

Use: For determination of the sterility of solutions containing mercu-

Preparation of Medium: Add 0.5mL of hemin solution and 0.1mL of vitamin K1 solution to 1.0L of thioglycolate medium without indi-

NaOH solution. Mix thoroughly. Bring volume to 100.0mL with distilled/deionized water.

1774

Thioglycolate Medium, Fluid

cator. Mix thoroughly. Distribute into screw-capped tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool tubes or flasks under 85% N2 + 10% H2 + 5% CO2. Tighten caps.

Use: For the isolation, cultivation, and identification of a wide variety of obligate anaerobic bacteria.

Thioglycolate Medium, Fluid (Fluid Thioglycolate Medium) (FTG) (BAM M146) Composition per liter: Pancreatic digest of casein .......................................................... 15.0g Glucose ......................................................................................... 5.0g Yeast extract.................................................................................. 5.0g NaCl .............................................................................................. 2.5g Agar ............................................................................................ 0.75g L-cystine ........................................................................................ 0.5g Sodium thioglycolate .................................................................... 0.5g Resazurin solution......................................................................1.0mL pH 7.1 ± 0.2 at 25°C

Resazurin Solution: Composition per 10.0mL: Na-resazurin............................................................................. 10.0mg

Preparation of Resazurin Solution: Add Na-resazurin to 10.0mL of distilled/deionized water. Mix thoroughly. Prepare freshly. Preparation of Medium: Add components, except sodium thioglycolate and resazurin solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Add 0.5g sodium thioglycolate. Mix thoroughly. Adjust pH to 7.1. Add 1.0mL resazurin solution. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 20 min at 15 psi pressure–121°C.

Use: For the cultivation of both aerobic and anaerobic organisms in the performance of sterility tests.

Thioglycolate Medium without Glucose

Thioglycolate Medium without Glucose Composition per liter: Pancreatic digest of casein.......................................................... 20.0g NaCl.............................................................................................. 2.5g K2HPO4......................................................................................... 1.5g Sodium thioglycolate .................................................................... 0.6g Agar .............................................................................................. 0.5g L-Cystine ....................................................................................... 0.4g Na2SO3 .......................................................................................... 0.2g Methylene Blue.......................................................................... 2.0mg pH 7.2 ± 0.2 at 25°C

Use: Use as a base for fermentation studies of anaerobic bacteria and for the promotion of endospore formation.

Thioglycolate Medium without Glucose and Indicator Composition per liter: Pancreatic digest of casein.......................................................... 15.0g Yeast extract.................................................................................. 5.0g NaCl.............................................................................................. 2.5g Agar ............................................................................................ 0.75g L-Cystine ..................................................................................... 0.25g Thioglycolic acid .......................................................................0.3mL pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. If medium becomes oxidized before use, heat in a boiling water bath to expel absorbed O2. Cool to 25°C.

Use: For the cultivation of anaerobic, microaerophilic, and aerobic microorganisms. For use in sterility testing of a variety of specimens.

Composition per liter: Pancreatic digest of casein .......................................................... 15.0g Yeast extract.................................................................................. 5.0g NaCl .............................................................................................. 2.5g Agar ............................................................................................ 0.75g L-Cystine ..................................................................................... 0.25g Methylene Blue.......................................................................... 2.0mg Thioglycolic acid .......................................................................0.3mL pH 7.2 ± 0.2 at 25°C

Thioglycolate Medium without Indicator Composition per liter:

Source: This medium is available as a premixed powder from BD Di-

Pancreatic digest of casein.......................................................... 15.0g Yeast extract.................................................................................. 5.0g Glucose ......................................................................................... 5.0g NaCl.............................................................................................. 2.5g Agar ............................................................................................ 0.75g Sodium thioglycolate .................................................................... 0.5g L-Cystine ..................................................................................... 0.25g pH 7.2 ± 0.2 at 25°C

agnostic Systems.

Source: This medium is available as a premixed powder from BD Di-

Preparation of Medium: Add components to distilled/deionized

agnostic Systems.

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. If medium becomes oxidized before use (Methylene Blue turns blue), heat in a boiling water bath to expel absorbed O2. Cool to 25°C.

Preparation of Medium: Add components to distilled/deionized

Use: For the cultivation of anaerobic, microaerophilic, and aerobic

microorganisms. For use in sterility testing of a variety of specimens.

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. If medium becomes oxidized before use, heat in a boiling water bath to expel absorbed O2. Cool to 25°C.

Thioglycolate Medium, USP

Thioglycolate Medium without Indicator Composition per liter: Pancreatic digest of casein .......................................................... 17.0g Glucose ......................................................................................... 6.0g Papaic digest of soybean meal ...................................................... 3.0g NaCl .............................................................................................. 2.5g Agar .............................................................................................. 0.7g Sodium thioglycolate .................................................................... 0.5g L-Cystine ..................................................................................... 0.25g Na2SO3 .......................................................................................... 0.1g pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath.

Use: For the growth of aerobic and anaerobic microorganisms in diagnostic bacteriology.

Thioglycolate Medium without Indicator-135C Composition per liter: Pancreatic digest of casein .......................................................... 17.0g Glucose ......................................................................................... 6.0g Papaic digest of soybean meal ...................................................... 3.0g NaCl .............................................................................................. 2.5g Agar .............................................................................................. 0.7g Sodium thioglycolate .................................................................... 0.5g Na2SO3 .......................................................................................... 0.1g L-Cystine ..................................................................................... 0.25g pH 7.0 ± 0.2 at 25°C

1775

Hemin ........................................................................................ 5.0mg Na2CO3 solution......................................................................10.0mL Vitamin K1 solution .................................................................10.0mL pH 7.2 ± 0.2 at 25°C

Na2CO3 Solution: Composition per 10.0mL: Na2CO3 ......................................................................................... 1.0g

Preparation of Na2CO3 Solution: Add Na2CO3 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Vitamin K1 Solution: Composition per 100.0mL: Vitamin K1 .................................................................................... 1.0g Ethanol, absolute......................................................................99.0mL

Preparation of Vitamin K1 Solution: Add vitamin K1 to 99.0mL of absolute ethanol. Mix thoroughly.

Preparation of Medium: Add components, except CaCO3, Na2CO3

solution, and vitamin K1 solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Add 0.1g of CaCO3 chips or powder to each of 100 test tubes. Distribute broth into the same tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 0.1mL of sterile Na2CO3 solution and 0.1mL of sterile vitamin K1 solution to each tube. Mix thoroughly.

Use: For the cultivation of a wide variety of obligate anaerobes. Thioglycolate Medium, Supplemented See: Thioglycolate Medium without Indicator with Hemin

Thioglycolate Medium, USP Composition per liter:

water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks, filling them half full. For maintenance of cultures, a small quantity of CaCO3 may be added to tubes before adding medium. Autoclave for 15 min at 13 psi pressure–118°C. Prepare freshly or boil and cool the medium just before use. Store prepared medium at 2°–8°C in the dark.

Pancreatic digest of casein.......................................................... 15.0g Glucose ......................................................................................... 5.5g Yeast extract.................................................................................. 5.0g NaCl.............................................................................................. 2.5g Agar .............................................................................................. 0.5g L-cystine ........................................................................................ 0.5g Sodium thioglycolate .................................................................... 0.5g Resazurin ................................................................................... 1.0mg pH 7.1 ± 0.2 at 25°C

Use: For the isolation and cultivation of a wide variety of microorgan-

Source: This medium is available as a premixed powder from Oxoid

isms, particularly obligate anaerobes, from clinical specimens and other materials.

Preparation of Medium: Add components to distilled/deionized

Source: This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium: Add components to distilled/deionized

Thioglycolate Medium without Indicator with Hemin (Thioglycolate Medium, Supplemented) Composition per liter: Pancreatic digest of casein .......................................................... 17.0g CaCO3, chips or powder ............................................................. 10.0g Glucose ......................................................................................... 6.0g Papaic digest of soybean meal ...................................................... 3.0g NaCl .............................................................................................. 2.5g Agar .............................................................................................. 0.7g Sodium thioglycolate .................................................................... 0.5g L-Cystine ..................................................................................... 0.25g Na2SO3 .......................................................................................... 0.1g © 2010 by Taylor and Francis Group, LLC

Unipath. water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of both aerobic and anaerobic organisms in the performance of sterility tests.

Thioglycolate Medium with Vitamin K1 and Hemin See: Thioglycolate Medium, Enriched Thioglycolate Peptone Glucose Yeast Extract Medium See: TPGY Medium Thioglycolate Potato Liver Medium See: TPL Medium

1776

Thiol Broth

Thiol Broth Composition per liter: Proteose peptone, No. 3 .............................................................. 10.0g Thiol complex ............................................................................... 8.0g Yeast extract.................................................................................. 5.0g NaCl .............................................................................................. 5.0g Glucose ......................................................................................... 1.0g p-Aminobenzoic acid .................................................................. 0.05g pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. For neutralization of penicillin, distribute medium into tubes to a depth of 60.0mm. For neutralization of streptomycin, distribute medium into tubes in shallow layers. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of bacteria from body fluids and other materials containing penicillin, streptomycin, or sulfonamides. Also used for the cultivation and maintenance of Bifidobacterium species.

Thiohalophilus Medium (DSMZ Medium 1058) Composition per liter: NaCl ......................................................................................... 120.0g K2HPO4 ......................................................................................... 1.5g NH4Cl .......................................................................................... 0.5g Bicarbonate solution ................................................................10.0mL Calcium chloride solution ........................................................10.0mL Magnesium chloride solution...................................................10.0mL Seven vitamin solution...............................................................1.0mL Trace elements solution SL-10 with EDTA ...............................1.0mL Carbonate solution .................................................................. variable pH 7.6 ± 0.2 at 25°C

Thiosulfate Solution: Composition per 10.0mL: Na2S2O3·5H2O .............................................................................. 5.0g

Preparation of Thiosulfate Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Sparge with 100% N2.

Calcium Chloride Solution: Composition per 10.0mL: CaCl2·2H2O ................................................................................ 0.05g

Preparation of Calcium Chloride Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Sparge with 100% N2 gas.

Seven Vitamin Solution: Composition per liter: Pyridoxine·HCl ...................................................................... 300.0mg Nicotinic acid......................................................................... 200.0mg Thiamine·HCl ........................................................................ 200.0mg Calcium DL-pantothenate....................................................... 100.0mg Cyanocobalamine .................................................................. 100.0mg p-Aminobenzoic acid............................................................... 80.0mg D(+)-Biotin............................................................................... 20.0mg

Preparation of Seven Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Sparge with 100% N2. Trace Elements Solution SL-10 with EDTA: Composition per liter: FeCl2·4H2O ................................................................................... 1.5g Na2-EDTA..................................................................................... 0.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Preparation of Trace Elements Solution SL-10 with EDTA: Add FeCl2·4H2O to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Adjust pH to 7.0.

Na2CO3 ......................................................................................... 2.0g

Preparation of Medium: Add components, except magnesium chloride, thiosulfate, bicarbonate, and vitamin solutions, to distilled/ deionized water and bring volume to 970.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2 gas mixture for at least 30 min to remove dissolved oxygen and to saturate the solution with CO2. Dispense into anaerobic culture vessels (e.g., Balch tubes) to 1/2 volume under air atmosphere. Close vials with butyl rubber septa to prevent free exchange of oxygen with the external atmosphere. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add magnesium chloride, thiosulfate, bicarbonate, and vitamin solutions. Mix thoroughly. Adjust pH to 7.5–7.8 using a sterile solution of sodium carbonate (5% w/v).

Preparation of Carbonate Solution: Add Na2CO3 to distilled/de-

Use: For the cultivation of Thiohalomonas denitrificans, Thiohalo-

Bicarbonate Solution: Composition per 10.0mL: NaHCO3 ........................................................................................ 5.0g

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with a gas mixture of 80% N2 + 20% CO2. Filter sterilize.

Carbonate Solution: Composition per 10.0mL:

ionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with a gas mixture of 80% N2 + 20% CO2. Filter sterilize.

Magnesium Chloride Solution: Composition per 10.0mL:

philus thiocyanatoxydans, and Thiomicrospira halophila.

Thiohalophilus Medium (DSMZ Medium 1058)

MgCl2·6H2O.................................................................................. 0.4g

Composition per liter:

Preparation of Magnesium Chloride Solution: Add 0.4g of

NaCl ......................................................................................... 233.0g K2HPO4......................................................................................... 1.5g

Thiohalophilus Medium

1777

NH4Cl .......................................................................................... 0.5g Bicarbonate solution ................................................................10.0mL Calcium chloride solution ........................................................10.0mL Magnesium chloride solution...................................................10.0mL Seven vitamins solution .............................................................1.0mL Trace elements solution SL-10 with EDTA ...............................1.0mL Carbonate solution .................................................................. variable pH 7.2 ± 0.2 at 25°C

MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O.............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Thiosulfate Solution: Composition per 10.0mL:

Add FeCl2·4H2O to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Adjust pH to 7.0.

Na2S2O3·5H2O .............................................................................. 5.0g

Preparation of Thiosulfate Solution: Add Na2S2O3·5H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize. Bicarbonate Solution: Composition per 10.0mL: NaHCO3 ........................................................................................ 5.0g

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with a gas mixture of 80% N2 + 20% CO2. Filter sterilize.

Carbonate Solution: Composition per 10.0mL: Na2CO3 ......................................................................................... 2.0g

Preparation of Carbonate Solution: Add Na2CO3 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with a gas mixture of 80% N2 + 20% CO2. Filter sterilize.

Magnesium Chloride Solution: Composition per 10.0mL: MgCl2·6H2O.................................................................................. 0.4g

Preparation of Magnesium Chloride Solution: Add 0.4g of MgCl2·6H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Sparge with 100% N2. Calcium Chloride Solution: Composition per 10.0mL: CaCl2·2H2O................................................................................. 0.05g

Preparation of Calcium Chloride Solution: Add CaCl2·2H2O

to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Sparge with 100% N2.

Seven Vitamin Solution: Composition per liter: Pyridoxine·HCl ...................................................................... 300.0mg Nicotinic acid ......................................................................... 200.0mg Thiamine·HCl ........................................................................ 200.0mg Calcium DL-pantothenate....................................................... 100.0mg Cyanocobalamine................................................................... 100.0mg p-Aminobenzoic acid............................................................... 80.0mg D(+)-Biotin ............................................................................... 20.0mg

Preparation of Seven Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Sparge with 100% N2.

Trace Elements Solution SL-10 with EDTA: Composition per liter: FeCl2·4H2O ................................................................................... 1.5g Na2-EDTA..................................................................................... 0.5g CoCl2·6H2O ........................................................................... 190.0mg © 2010 by Taylor and Francis Group, LLC

Preparation of Trace Elements Solution SL-10 with EDTA:

Preparation of Medium: Add components, except magnesium chloride, thiosulfate, bicarbonate, and vitamin solutions, to distilled/ deionized water and bring volume to 970.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2 gas mixture for at least 30 min to remove dissolved oxygen and to saturate the solution with CO2. Dispense into anaerobic culture vessels (e.g., Balch tubes) to 1/2 volume under air atmosphere. Close vials with butyl rubber septa to prevent free exchange of oxygen with the external atmosphere. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add magnesium chloride, thiosulfate, bicarbonate, and vitamin solutions. Mix thoroughly. Adjust pH to 7.2 using a sterile solution of sodium carbonate (5% w/v). Use: For the cultivation of Thiomicrospira halophila DSM 15071.

Thiohalophilus Medium (DSMZ Medium 1058) Composition per liter: NaCl ......................................................................................... 180.0g K2HPO4......................................................................................... 1.5g KNO3 ............................................................................................ 1.0g NH4Cl .......................................................................................... 0.5g Bicarbonate solution ................................................................10.0mL Calcium chloride solution........................................................10.0mL Magnesium chloride solution ..................................................10.0mL Seven vitamin solution ..............................................................1.0mL Trace elements solution SL-10 with EDTA...............................1.0mL Carbonate solution .................................................................. Variable pH 7.6 ± 0.2 at 25°C

Thiosulfate Solution: Composition per 10.0mL: Na2S2O3·5H2O .............................................................................. 5.0g

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with a gas mixture of 80% N2 + 20% CO2. Filter sterilize.

Carbonate Solution: Composition per 10.0mL: Na2CO3 ......................................................................................... 2.0g

Preparation of Carbonate Solution: Add Na2CO3 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with a gas mixture of 80% N2 + 20% CO2. Filter sterilize.

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Thiohalophilus Medium

Magnesium Chloride Solution: Composition per 10.0mL:

Thiohalophilus Medium (DSMZ Medium 1058)

MgCl2·6H2O.................................................................................. 0.4g

Composition per liter:

Preparation of Magnesium Chloride Solution: Add 0.4g of

NaCl ......................................................................................... 180.0g K2HPO4......................................................................................... 1.5g KNO3 ............................................................................................ 2.0g NH4Cl .......................................................................................... 0.5g Bicarbonate solution ................................................................10.0mL Calcium chloride solution........................................................10.0mL Magnesium chloride solution ..................................................10.0mL Seven vitamin solution ..............................................................1.0mL Trace elements solution SL-10 with EDTA...............................1.0mL Carbonate solution .................................................................. Variable pH 7.6 ± 0.2 at 25°C

MgCl2·6H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Sparge with 100% N2.

Calcium Chloride Solution: Composition per 10.0mL: CaCl2·2H2O................................................................................. 0.05g

Preparation of Calcium Chloride Solution: Add CaCl2·2H2O

to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Sparge with 100% N2.

Seven Vitamin Solution: Composition per liter: Pyridoxine·HCl ...................................................................... 300.0mg Nicotinic acid ......................................................................... 200.0mg Thiamine·HCl ........................................................................ 200.0mg Calcium DL-pantothenate....................................................... 100.0mg Cyanocobalamine................................................................... 100.0mg p-Aminobenzoic acid ............................................................... 80.0mg D(+)-Biotin ............................................................................... 20.0mg

Preparation of Seven Vitamin Solution: Add components to dis-

Thiosulfate Solution: Composition per 10.0mL: Na2S2O3·5H2O .............................................................................. 5.0g

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Sparge with 100% N2.

deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with a gas mixture of 80% N2 + 20% CO2. Filter sterilize.

Trace Elements Solution SL-10 with EDTA: Composition per liter:

Carbonate Solution: Composition per 10.0mL:

FeCl2·4H2O ................................................................................... 1.5g Na2-EDTA..................................................................................... 0.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O........................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution) ..................................................................10.0mL

Preparation of Medium: Add components, except magnesium chloride, thiosulfate, bicarbonate, and vitamin solutions, to distilled/ deionized water and bring volume to 970.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2 gas mixture for at least 30 min. to remove dissolved oxygen and to saturate the solution with CO2. Dispense into anaerobic culture vessels (e.g., Balch tubes) to 1/2 volume under air atmosphere. Close vials with butyl rubber septa to prevent free exchange of oxygen with the external atmosphere. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add magnesium chloride, thiosulfate, bicarbonate, and vitamin solutions. Mix thoroughly. Adjust pH to 7.5–7.8 using a sterile solution of sodium carbonate (5% w/v).

Na2CO3 ......................................................................................... 2.0g

Preparation of Carbonate Solution: Add Na2CO3 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with a gas mixture of 80% N2 + 20% CO2. Filter sterilize. Magnesium Chloride Solution: Composition per 10.0mL: MgCl2·6H2O ................................................................................. 0.4g

Calcium Chloride Solution: Composition per 10.0mL: CaCl2·2H2O ................................................................................ 0.05g

Preparation of Calcium Chloride Solution: Add CaCl2·2H2O

to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Sparge with 100% N2.

Thiomicrospira denitrificans Agar Preparation of Seven Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Sparge with 100% N2. Trace Elements Solution SL-10 with EDTA: Composition per liter: FeCl2·4H2O ................................................................................... 1.5g Na2-EDTA..................................................................................... 0.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O........................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution) ..................................................................10.0mL

Preparation of Medium: Add components, except magnesium chloride, thiosulfate, bicarbonate, and vitamin solutions, to distilled/ deionized water and bring volume to 970.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2 gas mixture for at least 30 min. to remove dissolved oxygen and to saturate the solution with CO2. Dispense into anaerobic culture vessels (e.g., Balch tubes) to 1/2 volume under air atmosphere. Close vials with butyl rubber septa to prevent free exchange of oxygen with the external atmosphere. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add magnesium chloride, thiosulfate, bicarbonate, and vitamin solutions. Mix thoroughly. Adjust pH to 7.5–7.8 using a sterile solution of sodium carbonate (5% w/v). Use: For the cultivation of Thiohalomonas nitratireducens.

Thiol HiVeg Broth Composition per liter: Plant peptone No. 3..................................................................... 10.0g Thiol compound ............................................................................ 8.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g Glucose ......................................................................................... 1.0g p-Aminobenzoic acid (PABA) .................................................... 0.05g pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. For neutralization of penicillin, distribute medium into tubes to a depth of 60.0mm. For neutralization of streptomycin, distribute medium into tubes in shallow layers. Autoclave for 15 min at 15 psi pressure–121°C.

Thiol HiVeg Medium Composition per liter: Plant peptone No. 3..................................................................... 10.0g Thiol compound ............................................................................ 8.0g © 2010 by Taylor and Francis Group, LLC

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Yeast extract.................................................................................. 5.0g NaCl.............................................................................................. 5.0g Agar .............................................................................................. 1.0g Glucose ......................................................................................... 1.0g p-Aminobenzoic acid (PABA).................................................... 0.05g pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. For neutralization of penicillin, distribute medium into tubes to a depth of 60 mm. For neutralization of streptomycin, distribute medium into tubes in shallow layers. Autoclave for 15 min at 15 psi pressure–121°C.

Thiol Medium Composition per liter: Proteose peptone No. 3 ............................................................... 10.0g Thiol complex............................................................................... 8.0g Yeast extract.................................................................................. 5.0g NaCl.............................................................................................. 5.0g Glucose ......................................................................................... 1.0g Agar .............................................................................................. 1.0g p-Aminobenzoic acid.................................................................. 0.05g pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. For neutralization of penicillin, distribute medium into tubes to a depth of 60mm. For neutralization of streptomycin, distribute medium into tubes in shallow layers. Autoclave for 15 min at 15 psi pressure–121°C.

Thiomicrospira denitrificans Agar Composition per 1001.0mL: Solution A..............................................................................940.0mL Solution B ................................................................................40.0mL Solution C ................................................................................20.0mL Solution D..................................................................................1.0mL pH 7.0 ± 0.2 at 25°C

Solution A: Composition per 940.0mL: Agar ............................................................................................ 15.0g KH2PO4......................................................................................... 2.0g KNO3 ............................................................................................ 2.0g NH4Cl ........................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.8g Trace elements solution SL-4 ....................................................2.0mL

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Gently heat and

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Thiomicrospira denitrificans Broth

bring to boiling. Adjust pH to 7.0 with NaOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Solution A: Composition per 940.0mL:

Trace Elements Solution SL-4: Composition per liter:

KH2PO4......................................................................................... 2.0g KNO3 ............................................................................................ 2.0g NH4Cl ........................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.8g Trace elements solution SL-4 ....................................................2.0mL

EDTA ............................................................................................ 0.5g FeSO4·7H2O.................................................................................. 0.2g Trace elements solution SL-6 ..................................................... 100.0

Preparation of Trace Elements Solution SL-4: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Trace Elements Solution SL-6: Composition per liter: H3BO3 ........................................................................................... 0.3g CoCl2·6H2O .................................................................................. 0.2g ZnSO4·7H2O ................................................................................. 0.1g MnCl2·4H2O................................................................................ 0.03g Na2MoO4·H2O ............................................................................ 0.03g NiCl2·6H2O ................................................................................. 0.02g CuCl2··2H2O................................................................................ 0.01g

Preparation of Trace Elements Solution SL-6: Add components

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Adjust pH to 7.0 with NaOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Solution B: Composition per 40.0mL: Na2S2O3·5H2O .............................................................................. 5.0g

Preparation of Solution B: Add Na2S2O3·5H2O to distilled/deion-

ized water and bring volume to 40.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Solution C: Composition per 20.0mL:

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3.4.

NaHCO3 ........................................................................................ 1.0g

Solution B: Composition per 40.0mL:

water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize.

Na2S2O3·5H2O .............................................................................. 5.0g

Preparation of Solution B: Add Na2S2O3·5H2O to distilled/deion-

ized water and bring volume to 40.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Solution C: Composition per 20.0mL: NaHCO3 ........................................................................................ 1.0g

Preparation of Solution C: Add NaHCO3 to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize.

Solution D: Composition per liter: FeSO4·7H2O............................................................................... 2.0mg H2SO4 (0.1N solution) ...............................................................1.0mL

Preparation of Solution D: Add FeSO4·7H2O to 1.0mL of 0.1N

H2SO4 solution. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Preparation of Solution C: Add NaHCO3 to distilled/deionized Solution D: Composition per liter: FeSO4·7H2O............................................................................... 2.0mg H2SO4 (0.1N solution) ...............................................................1.0mL

Preparation of Solution D: Add FeSO4·7H2O to 1.0mL of 0.1N

H2SO4 solution. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Preparation of Trace Elements Solution SL-4: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Trace Elements Solution SL-6: Composition per liter:

Use: For the cultivation and maintenance of Thiomicrospira denitrifi-

H3BO3 ........................................................................................... 0.3g CoCl2·6H2O .................................................................................. 0.2g ZnSO4·7H2O ................................................................................. 0.1g MnCl2·4H2O ............................................................................... 0.03g Na2MoO4·H2O ............................................................................ 0.03g NiCl2·6H2O ................................................................................. 0.02g CuCl2·2H2O................................................................................. 0.01g

cans.

Preparation of Trace Elements Solution SL-6: Add components to

Preparation of Medium: Aseptically add 40.0mL of sterile solution B, 20.0mL of sterile solution C, and 1.0mL of sterile solution D to 940.0mL of sterile solution A. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes under 100% N2.

Thiomicrospira denitrificans Broth

distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3.4.

Composition per 1001.0mL:

Preparation of Medium: Aseptically add 40.0mL of sterile solution

Solution A ..............................................................................940.0mL Solution B ................................................................................40.0mL Solution C ................................................................................20.0mL Solution D ..................................................................................1.0mL pH 7.0 ± 0.2 at 25°C

B, 20.0mL of sterile solution C, and 1.0mL of sterile solution D to 940.0mL of sterile solution A. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes under 100% N2.

Use: For the cultivation and maintenance of Thiomicrospira denitrificans.

Thiomicrospira Medium

Thiomicrospira denitrificans Medium Composition per liter: Part I.......................................................................................500.0mL Part II .....................................................................................500.0mL pH 7.0–8.0 at 25°C

Part I: Composition per liter: NaCl ............................................................................................ 20.0g KNO3 ............................................................................................ 4.0g (NH4)2SO4 ..................................................................................... 2.0g MgSO4·7H2O ................................................................................ 1.5g K2HPO4 ......................................................................................... 0.6g KH2PO4 ......................................................................................... 0.4g FeSO4 solution ...........................................................................2.0mL Trace metals solution .................................................................2.0mL HCl, concentrated ......................................................................1.0mL

Preparation of Part I: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. FeSO4 Solution: Composition per 100.0mL: FeSO4·7H2O.................................................................................. 0.5g HCl (1N solution)...................................................................100.0mL

Preparation of FeSO4 Solution: Combine the FeSO4·7H2O and 100.0mL of HCl solution. Mix thoroughly.

Trace Metals Solution: Composition per liter: Disodium EDTA ......................................................................... 50.0g NaOH .......................................................................................... 11.0g CaCl2·2H2O................................................................................. 7.34g MnCl2·2H2O.................................................................................. 2.5g ZnSO4·7H2O ................................................................................. 2.2g CoCl2·6H2O .................................................................................. 0.5g (NH4)6Mo7O24·4H2O .................................................................... 0.5g CuSO4·5H2O ................................................................................. 0.2g

Part II: Composition per liter: Na2S2O3·5H2O ............................................................................ 10.0g NaHCO3 ........................................................................................ 3.0g NaOH .......................................................................................... 0.05g

Preparation of Part II: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Preparation of Medium: Aseptically combine 500.0mL of sterile part I and 500.0mL of sterile part II. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the isolation and cultivation of Thiomicrospira denitrificans.

Thiomicrospira Medium (ATCC Medium 1036) Composition per liter: NaCl ............................................................................................ 25.0g Na2S2O3·5H2O .............................................................................. 8.0g © 2010 by Taylor and Francis Group, LLC

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MgSO4·7H2O ................................................................................ 1.5g (NH4)2SO4 .................................................................................... 1.0g K2HPO4......................................................................................... 0.5g CaCl2 ............................................................................................. 0.3g Vitamin B12 ............................................................................... 15.0μg Vishniac and Santer trace metals ...............................................0.2mL Bromcresol Purple (0.05% solution) .........................................0.1mL pH 7.2 ± 0.2 at 25°C

Vishniac and Santer Trace Metals: Composition per liter: Ethylenediamine tetraacetic acid (EDTA) .................................. 50.0g ZnSO4·7H2O ............................................................................... 22.0g CaCl2 ........................................................................................... 5.54g MnCl2·4H2O ............................................................................... 5.06g FeSO4·7H2O................................................................................ 4.99g CoCl2·6H2O ................................................................................ 1.61g CuSO4·5H2O............................................................................... 1.57g (NH4)6Mo7O24·4H2O .................................................................... 1.1g

Use: For the cultivation and maintenance of Thiomicrospira species.

Thiomicrospira Medium (ATCC Medium 1422) Composition per liter: NaCl............................................................................................ 25.1g Tris·HCl ...................................................................................... 3.07g Na2S2O3·5H2O ............................................................................ 2.48g MgSO4·7H2O ................................................................................ 1.5g (NH4)2SO4 .................................................................................... 1.0g KH2PO4....................................................................................... 0.42g CaCl2·2H2O ................................................................................ 0.29g NaHCO3 ........................................................................................ 0.2g Phenol Red (0.5% solution).......................................................1.0mL Vishniac and Santer trace metals ...............................................0.2mL pH 7.5 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Thiomicrospira species.

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Thiomicrospira pelophila Medium

Thiomicrospira pelophila Medium Composition per liter: NaCl ............................................................................................ 25.0g Agar ............................................................................................ 10.0g Na2S2O3·5H2O ....................................................................... 5.0–8.0g MgSO4·7H2O ................................................................................ 1.5g (NH4)2SO4 ..................................................................................... 1.0g K2HPO4 ......................................................................................... 0.5g CaCl2 ............................................................................................. 0.3g Vitamin B12 .............................................................................. 0.15mg Trace metals solution .................................................................0.2mL

Trace Metals Solution: Composition per liter: Disodium EDTA ......................................................................... 50.0g NaOH .......................................................................................... 11.0g CaCl2·2H2O................................................................................. 7.34g FeSO4·7H2O.................................................................................. 5.0g MnCl2·2H2O.................................................................................. 2.5g ZnSO4·7H2O ................................................................................. 2.2g CoCl2·6H2O .................................................................................. 0.5g (NH4)6Mo7O24·4H2O .................................................................... 0.5g CuSO4·5H2O ................................................................................. 0.2g

Use: For the cultivation of Thiomicrospira pelophila.

Thiomicrospira pelophila Medium Composition per 1000.2mL: NaCl ............................................................................................ 25.0g MgSO4·7H2O ............................................................................... 1.5g (NH4)2SO4 ..................................................................................... 1.0g CaCl2·2H2O................................................................................. 0.42g Bromthymol Blue ...................................................................... 4.0mg K2HPO4 solution ....................................................................100.0mL Na2S2O3·5H2O solution .........................................................100.0mL Vitamin B12 solution ................................................................10.0mL Trace elements solution .............................................................0.2mL Na2CO3 solution ..................................................................... variable pH 7.2 ± 0.2 at 25°C

K2HPO4 Solution: Composition per 100.0mL: K2HPO4 ......................................................................................... 0.5g

Preparation of K2HPO4 Solution: Add K2HPO4 to distilled/de-

ionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Na2S2O3 Solution: Composition per 10.0mL: Na2S2O3 ·5H2O.............................................................................. 5.0g

Preparation of Na2S2O3 Solution: Add Na2S2O3·5H2O to dis-

Vitamin B12 Solution: Composition per 10.0mL: Vitamin B12 .............................................................................. 15.0mg

Preparation of Vitamin B12 Solution: Add vitamin B12 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Trace Elements Solution: Composition per liter: Disodium EDTA ......................................................................... 50.0g ZnSO4·7H2O .............................................................................. 22.0g CaCl2·2H2O ................................................................................ 5.54g MnCl2·4H2O ............................................................................... 5.06g FeSO4·7H2O.................................................................................. 5.0g CoCl2·6H2O ................................................................................ 1.61g CuSO4·5H2O ............................................................................... 1.57g (NH4)6Mo7O24·4H2O .................................................................... 1.1g

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Na2CO3 Solution: Composition per 100.0mL: Na2CO3 ......................................................................................... 0.4g

Preparation of Na2CO3 Solution: Add Na2CO3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Preparation of Medium: Add components, except K2HPO4 solution, Na2S2O3·5H2O solution, vitamin B12 solution, and Na2CO3 solution, to distilled/deionized water and bring volume to 790.0mL. Mix thoroughly. Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure– 121°C. Aseptically add 100.0mL of sterile K2HPO4 solution, 100.0mL of sterile Na2S2O3·5H2O solution, and 10.0mL of sterile vitamin B12 solution. Mix thoroughly. Aseptically adjust pH to 7.2 with the appropriate volume of sterile Na2CO3 solution. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation and maintenance of Thiomicrospira pelophila.

Thiomonas delicata Medium (DSMZ Medium 1037) Composition per liter: Na2S2O3·5H2O .............................................................................. 5.0g Na2HPO4 ....................................................................................... 4.5g K2HPO4......................................................................................... 1.5g Yeast extract ................................................................................. 1.0g Na-aspartate ................................................................................. 1.0g NH4Cl .......................................................................................... 0.3g MgSO4·7H2O ................................................................................ 0.1g pH 6.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0. Dispense into culture vessels. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation of Thiomonas delicata.

Thiorhodococcus Medium

K2HPO4 ......................................................................................... 1.5g Yeast extract ................................................................................. 1.0g NH4Cl .......................................................................................... 0.3g MgSO4·7H2O ................................................................................ 0.1g pH 6.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

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MnCl2·4H2O ................................................................................. 0.1g ZnCl2 ........................................................................................... 0.07g H3BO3 ......................................................................................... 0.06g NaMoO4·2H2O............................................................................ 0.04g CuCl2·2H2O ................................................................................ 0.02g NiCl2·6H20.................................................................................. 0.02g

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0. Dispense into culture vessels. Autoclave for 15 min at 15 psi pressure– 121°C.

Preparation of Trace Elements Solution SL-8: Add compo-

Use: For the cultivation of Thiomonas perometabolis.

Preparation of Medium: Add components, except sulfide, bicarbonate, thiosulfate, and vitamin solutions, to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2 gas mixture. Dispense into culture vessels. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add sulfide, thiosulfate, bicarbonate, and vitamin solutions. Mix thoroughly. Adjust pH to 7.5.

Thiophaeococcus mangrovi Medium (DSMZ Medium 1162) Composition per liter: NaCl ............................................................................................ 20.0g Sodium pyruvate ........................................................................... 3.0g MgSO4·7H2O ................................................................................ 2.0g NH4Cl ........................................................................................ 0.64g K2HPO4 ......................................................................................... 0.5g Yeast extract ................................................................................. 0.4g CaCl2·2H2O................................................................................. 0.15g Bicarbonate solution ................................................................10.0mL Sulfide solution ........................................................................10.0mL Thiosulfate solution .................................................................10.0mL Trace element solution SL-8 ......................................................1.0mL Vitamin solution.........................................................................1.0mL pH 7.5 ± 0.2 at 25°C

Sulfide Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 1.0g

Preparation of Sulfide Solution: Add Na2S·9H2O to distilled/de-

nents to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Use: For the cultivation of Thiophaeococcus mangrovi. Thiophene-2-Carboxylic Acid Hydrazide See: TCH Medium

Thiorhodococcus Medium (DSMZ Medium 28a) Composition per 5.0L: Solution A.....................................................................................4.0L Solution B ..............................................................................860.0mL Solution E ..............................................................................100.0mL Solution F.................................................................................20.0mL Solution C ..................................................................................5.0mL Solution D..................................................................................5.0mL pH 7.3 at 25°C

ionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Solution A: Composition per 4.0L:

Thiosulfate Solution: Composition per 10.0mL:

NaCl.......................................................................................... 100.0g MgCl2·6H2O ................................................................................. 5.0g MgSO4 .......................................................................................... 2.5g Na2S2O3·5H2O .............................................................................. 2.5g KH2PO4......................................................................................... 1.7g NH4Cl ........................................................................................... 1.7g KCl................................................................................................ 1.7g CaCl2·2H2O ................................................................................ 1.25g

Na2S2O3·5H2O .............................................................................. 1.5g

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with a gas mixture of 80% N2 + 20% CO2. Filter sterilize.

Vitamin Solution: Composition per 10.0mL: Vitamin B12 ................................................................................ 2.0mg

Preparation of Vitamin Solution: Add Vitamin B12 to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Trace Elements Solution SL-8: Composition per liter: Disodium EDTA ........................................................................... 5.2g FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ................................................................................ 0.19g © 2010 by Taylor and Francis Group, LLC

Preparation of Solution A: Add components to 4.0L distilled water. Mix thoroughly. Autoclave for 45 min at 15 psi pressure–121°C in a 5-liter special bottle or flask with four openings at the top, together with a teflon-coated magnetic bar. In this 5-liter bottle, two openings for tubes are in the central, silicon rubber stopper; one is a short, gasinlet tube with a sterile cotton filter, and the other is an outlet tube for medium, which reaches the bottom of the vessel at one end and has, at the other end, a silicon rubber tube with a pinch cock and a bell for aseptic dispensing of the medium into bottles. The other two openings have gas-tight screw caps; one of these openings is for the addition of sterile solutions and the other serves as a gas outlet. After autoclaving, cool to room temperature under a 100% N2 atmosphere with a positive pressure of 0.05– 0.1 atm (a manometer for low pressure will be required). Saturate the cold medium with CO2 by magnetic stirring for 30 min under a CO2 atmosphere of 0.05–0.1 atm. Solution B: Distilled water........................................................................860.0mL

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Thiosphaera Agar

Preparation of Solution B: Autoclave distilled water for 15 min at 15 psi pressure–121°C in a cotton-stoppered Erlenmeyer flask. Cool to room temperature under an atmosphere of N2 in an anaerobic jar.

Solution C: Composition per 100.0mL: Vitamin B12 ................................................................................ 2.0mg

Preparation of Solution C: Add vitamin B12 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Sparge under 100% N2 gas for 3 min. Filter sterilize Store under N2 gas. Solution D: Composition per liter: Disodium ethylendiamine tetraacetate (Disodium EDTA) ........... 3.0g FeSO4·7H2O................................................................................. 1.1g H3BO3 ........................................................................................... 0.3g CoCl2·6H2O ................................................................................ 0.19g MnCl2·2H2O............................................................................. 50.0mg ZnCl2 ........................................................................................ 42.0mg NiCl2·6H2O .............................................................................. 24.0mg Na2MoO4·2H2O ...................................................................... 18.0mg CuCl2·2H2O ............................................................................... 2.0mg

Preparation of Solution D: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Solution E: Composition per 100.0mL: NaHCO3 ........................................................................................ 7.5g

Preparation of Solution E: Add NaHCO3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Sparge with 100% CO2 until saturated. Filter sterilize under 100% CO2 into a sterile, gas-tight 100.0mL screw-capped bottle.

Solution F: Composition per 100.0mL: Na2S·9H2O .................................................................................. 10.0g

Preparation of Solution F: Add Na2S·9H2O to distilled/deionized

water in a 250.0mL screw-capped bottle fitted with a butyl rubber septum and bring volume to 100.0mL. Mix thoroughly. Sparge under 100% N2 gas for 3 min. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to room temperature.

Neutralized Sulfide Solution: Composition per 100.0mL: Na2S·9H2O .................................................................................... 1.5g

Preparation of Neutralized Sulfide Solution: Add Na2S·9H2O

to distilled/deionized water in a 250.0mL screw-capped bottle fitted with a butyl rubber septum and bring volume to 100.0mL. Add a magnetic stir bar. Mix thoroughly. Sparge under 100% N2 gas for 3 min. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Adjust pH to about 7.3 with sterile 2M H2SO4. Do not open the bottle to add H2SO4; use a sterile syringe. Stir the solution continuously to avoid precipitation of elemental sulfur. The final solution should be clear and yellow in color.

Preparation of Medium: Add solutions B, C, D, E, and F to solution A through one of the screw-cap openings against a stream of either N2 gas or, better, a mixture of 95% N2 and 5% CO2 while the medium is magnetically stirred. Adjust the pH of the medium with sterile HCl or Na2CO3 solution (2M solutions) to pH 7.3. Distribute the medium aseptically through the medium outlet tube into sterile, 100mL bottles (with metal caps and autoclavable rubber seals) using the positive gas © 2010 by Taylor and Francis Group, LLC

pressure (0.05–0.1 atm) of the N2 /CO2 gas mixture. Leave a small air bubble in each bottle to meet possible pressure changes. The tightly sealed, screw-cap bottles can be stored for several weeks or months in the dark. During the first 24 hr, the iron of the medium precipitates in the form of black flocs. No other sediment should arise in the otherwise clear medium. Incubate in the light at 500–1,000 lux intensity. Feed periodically with neutralized solution of sodium sulfide to replenish sulfide and with other supplement solutions.

Use: For the cultivation of Thiorhodococcus minor.

Thiosphaera Agar Composition per liter: Agar ............................................................................................ 15.0g Na2HPO4 ....................................................................................... 4.2g KH2PO4......................................................................................... 1.5g NH4Cl ........................................................................................... 0.3g MgSO4·7H2O ................................................................................ 0.1g KNO3 ............................................................................................ 0.1g Vishniac and Santer trace metals ...............................................2.0mL pH 8.0–8.2 at 25°C

Vishniac and Santer Trace Metals: Composition per liter: Ethylenediamine tetraacetic acid (EDTA) .................................. 50.0g ZnSO4·7H2O ............................................................................... 22.0g CaCl2 ........................................................................................... 5.54g MnCl2·4H2O ............................................................................... 5.06g FeSO4·7H2O................................................................................ 4.99g CoCl2·6H2O ................................................................................ 1.61g CuSO4·5H2O ............................................................................... 1.57g (NH4)6Mo7O24·4H2O .................................................................... 1.1g

Preparation of Vishniac and Santer Trace Metals: Add components to distilled/deionized water and bring volume to 1.0L. Adjust pH to 6.0 with KOH. Mix thoroughly. Preparation of Medium: Add components, except agar, to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 8.0–8.2. Filter sterilize. Warm to 45°–50°C. Add agar to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically combine the two sterile solutions. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Thiosphaera pantotropha.

Thiosphaera Broth Composition per liter: Na2HPO4 ....................................................................................... 4.2g KH2PO4......................................................................................... 1.5g NH4Cl ........................................................................................... 0.3g MgSO4·7H2O ................................................................................ 0.1g KNO3 ............................................................................................ 0.1g Vishniac and Santer trace metals ...............................................2.0mL pH 8.0–8.2 at 25°C

Thiosulfate Salts Broth

FeSO4·7H2O................................................................................ 4.99g CoCl2·6H2O ................................................................................ 1.61g CuSO4·5H2O ............................................................................... 1.57g (NH4)6Mo7O24·4H2O .................................................................... 1.1g

Preparation of Vishniac and Santer Trace Metals: Add components to distilled/deionized water and bring volume to 1.0L. Adjust pH to 6.0 with KOH. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.0–8.2. Filter sterilize. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Thiosphaera pantotropha.

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Thiosulfate-Oxidizing Medium Composition per liter: K2HPO4......................................................................................... 2.0g MgSO4·7H2O ................................................................................ 0.1g CaCl2·2H2O .................................................................................. 0.1g FeCl3·6H2O................................................................................. 0.02g (NH4)2SO4 solution................................................................100.0mL Thiosulfate solution ...............................................................100.0mL pH 7.8 ± 0.2 at 25°C

(NH4)2SO4 Solution: Composition per 100.0mL: (NH4)2SO4 .................................................................................... 0.1g

Preparation of (NH4)2SO4 Solution: Add the (NH4)2SO4 to dis-

Thiosphaera pantotropha Medium Composition per 1001.0mL: Agar ............................................................................................ 20.0g Na2HPO4·2H2O............................................................................ .7.9g KH2PO4 ......................................................................................... 1.5g NH4Cl ........................................................................................... 0.3g MgSO4·7H2O ................................................................................ 0.1g Yeast extract solution ...............................................................10.0mL Trace elements solution SL-10 ..................................................1.0mL pH 7.5 ± 0.2 at 25°C

Yeast Extract Solution: Composition per 10.0mL: Yeast extract.................................................................................. 1.0g

Preparation of Yeast Extract Solution: Add yeast extract to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Trace Elements Solution SL-10: Composition per liter: FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O........................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution) ..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

Preparation of Medium: Add components, except yeast extract solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 10.0mL of sterile yeast extract solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Thiosulfate Solution: Composition per 100.0mL: Na2S2O3·5H2O ............................................................................ 10.0g

Preparation of Thiosulfate Solution: Add the Na2S2O3·5H2O to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Preparation of Medium: Add components, except (NH4)2SO4 solution and thiosulfate solution, to distilled/deionized water and bring volume to 800.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add the sterile (NH4)2SO4 solution and the sterile thiosulfate solution. Mix thoroughly. Adjust the pH to 7.8 if necessary. Aseptically distribute into sterile tubes or flasks. Use: For the isolation and cultivation of iron and sulfur bacteria.

Thiosulfate Salts Broth Composition per liter: Na2S2O3·5H2O .......................................................................... 24.81g NH4·Cl .......................................................................................... 2.2g KH2PO4......................................................................................... 2.0g Artificial seawater..................................................................500.0mL

Artificial Seawater: Composition per liter: NaCl........................................................................................ 23.476g MgCl2........................................................................................ 4.981g Na2SO4 ...................................................................................... 3.917g CaCl2 ......................................................................................... 1.102g KCl............................................................................................ 0.664g NaHCO3 .................................................................................... 0.192g KBr ........................................................................................... 0.096g H3BO3 ....................................................................................... 0.026g SrCl3.......................................................................................... 0.024g NaF ............................................................................................ 3.0mg pH 5.0 ± 0.2 at 25°C

Preparation of Artificial Seawater: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation of Thiobacillus species.

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Thiothrix Agar

Thiothrix Agar (DSMZ Medium 573) Composition per liter: Agar ............................................................................................ 12.0g NH4Cl ........................................................................................... 0.2g Na-acetate ..................................................................................... 0.1g K2HPO4 ....................................................................................... 0.01g MgSO4·7H2O .............................................................................. 0.01g CaSO4 (saturated solution)......................................................20.0mL Na2S·9H2O solution .................................................................10.0mL Trace elements solution .............................................................5.0mL pH 7.5 ± 0.2 at 25°C

Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.3g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

tilled/deionized water and bring volume to 10.0mL. Sparge with N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Store anaerobically.

Trace Elements Solution: Composition per liter: FeSO4·7H2O.................................................................................. 0.7g EDTA ............................................................................................ 0.2g ZnSO4·7H2O ............................................................................ 10.0mg H3BO3 ...................................................................................... 10.0mg MnSO4·4H2O ............................................................................. 2.0mg Co(NO3)2 .................................................................................. 1.0mg Na2MoO4·4H2O ......................................................................... 1.0mg CuSO4·5H2O ...............................................................................5.0µg

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, except Na2S·9H2O so-

lution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 10.0mL sterile Na2S·9H2O solution. Mix thoroughly. Pour into Petri dishes or aseptically distribute into sterile tubes.

Use: For the cultivation and maintenance of Thiothrix nivea.

Thiothrix Agar Composition per 1003.0mL: Agar ............................................................................................ 12.0g NH4Cl ........................................................................................... 0.2g Sodium acetate .............................................................................. 0.1g K2HPO4 ....................................................................................... 0.01g MgSO4·7H2O .............................................................................. 0.01g CaSO4 (saturated solution).......................................................20.0mL Na2S·9H2O solution ...................................................................3.0mL Trace elements solution .............................................................5.0mL pH 7.5 ± 0.2 at 25°C

Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 1.0g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-

Trace Elements Solution: Composition per liter: FeSO4·7H2O.................................................................................. 0.7g EDTA ............................................................................................ 0.2g ZnSO4·7H2O ............................................................................... 0.01g MnSO4·4H2O ............................................................................ 0.002g H3BO3 ...................................................................................... 10.0mg CO(NO3)2 .................................................................................. 1.0mg Na2MoO4·2H2O ........................................................................ 1.0mg CuSO4·5H2O ............................................................................... 5.0μg

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, except Na2S·9H2O solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 3.0mL of sterile Na2S·9H2O solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation and maintenance of Thiothrix nivea.

Thiothrix Medium (DSMZ Medium 573) Composition per liter: NH4Cl ........................................................................................... 0.2g Na-acetate ..................................................................................... 0.1g K2HPO4....................................................................................... 0.01g MgSO4·7H2O .............................................................................. 0.01g CaSO4 (saturated solution)......................................................20.0mL Na2S·9H2O solution .................................................................10.0mL Trace elements solution .............................................................5.0mL pH 7.5 ± 0.2 at 25°C

Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O.................................................................................... 0.3g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Sparge with N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Store anaerobically. Trace Elements Solution: Composition per liter: FeSO4·7H2O.................................................................................. 0.7g EDTA ............................................................................................ 0.2g ZnSO4·7H2O ............................................................................ 10.0mg H3BO3 ...................................................................................... 10.0mg MnSO4·4H2O ............................................................................. 2.0mg Co(NO3)2 .................................................................................. 1.0mg Na2MoO4·4H2O ......................................................................... 1.0mg CuSO4·5H2O ............................................................................... 5.0µg

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, except Na2S·9H2O solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0mL sterile Na2S·9H2O solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation and maintenance of Thiothrix nivea.

Tindallia Medium

Thorne Medium, Modified Composition per liter: Glycerol ...................................................................................... 20.0g L-Glutamic acid............................................................................. 4.0g Citric acid...................................................................................... 2.0g MgSO4·7H2O ................................................................................ 1.0g Ferric ammonium citrate............................................................... 0.5g K2HPO4 ......................................................................................... 0.5g pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH using NH4OH (not NaOH). Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Bacillus licheniformis. Thymidine Auxotroph XPS Medium See: XPS Broth with Thymidine

Tibi Medium Composition per liter: Sucrose........................................................................... 100.0–150.0g Fig, dried, quartered........................................................................... 1 Lemon wedge (0.5cm segment)......................................................... 1

Preparation of Medium: Add components to tap water and bring volume to 1.0L in a 1.0L Erlenmeyer flask fitted with a cotton stopper. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Inoculate with about 50.0mL of Tibi grains.

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KCl................................................................................................ 0.2g K2HPO4......................................................................................... 0.2g Na2S·9H2O solution .................................................................10.0mL Trace elements solution SL-10 ..................................................1.0mL Vitamin solution.........................................................................1.0mL pH 9.0 ± 0.2 at 25°C

Vitamin Solution: Composition per liter: Pyridoxine-HCl........................................................................ 10.0mg Thiamine-HCl·2H2O.................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg D-Ca-pantothenate ..................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize. Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O.................................................................................... 0.5g

Use: For the cultivation of osmophilic bacteria and fungi from tibi

grains.

Preparation of Medium: Prepare and dispense medium under

Tieghemiomyces Medium Composition per liter: Agar ............................................................................................ 20.0g Casein hydrolysate ...................................................................... 10.0g KH2PO4 ......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g ZnSO4·7H2O .............................................................................. 0.2mg FeSO4 ......................................................................................... 0.2mg MnSO4·H2O ............................................................................... 0.2mg Thiamine .................................................................................... 0.2mg Biotin ....................................................................................... 0.01mg Glycerol ...................................................................................20.0mL pH 6.0–6.5 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 6.0–6.5. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Tieghemiomyces parasiticus.

Tindallia Medium (DSMZ Medium 798) Composition per liter: NaCl ............................................................................................ 10.0g Na2CO3 ......................................................................................... 8.0g Yeast extract.................................................................................. 4.0g NH4Cl ........................................................................................... 0.5g © 2010 by Taylor and Francis Group, LLC

100% N2. Add components, except vitamin solution, to distilled/deionized water and bring volume to 999.0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for 5 min. Cool to 25°C while sparging with 100% N2. Adjust pH to 9.0. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 25°C. Aseptically and anaerobically add 1.0mL vitamin solution. Mix thoroughly. Aseptically and anaerobically under 100% N2 distribute into sterile tubes or bottles.

Use: For the cultivation of Tindallia magadiensis.

Tindallia Medium (DSMZ Medium 1148) Composition per liter: NaCl ........................................................................................... 30.0g Peptone ........................................................................................ 5.0g NH4Cl .......................................................................................... 1.0g Yeast extract ................................................................................. 0.5g KCl ............................................................................................... 0.2g K2HPO4......................................................................................... 0.2g MgCl2·6H2O ................................................................................. 0.1g Resazurin ................................................................................... 1.0mg Carbonate solution ...................................................................10.0mL Bicarbonate solution ................................................................10.0mL Sulfide solution........................................................................10.0mL Vitamin solution.........................................................................2.0mL Trace elements solution .............................................................1.0mL pH 9.7 ± 0.2 at 25°C

Sulfide Solution: Composition per 10.0mL: Na2S·9H2O.................................................................................. 0.25g

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Tinsdale Agar

Preparation of Sulfide Solution: Add Na2S·9H2O to distilled/de-

ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Bicarbonate Solution: Composition per 10.0mL: NaHCO3 ........................................................................................ 1.5g

Preparation of Bicarbonate Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with a gas mixture of 80% N2 + 20% CO2. Filter sterilize. Carbonate Solution: Composition per 10.0mL: Na2CO3 ....................................................................................... 2.76g

Preparation of Carbonate Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with a gas mixture of 80% N2 + 20% CO2. Filter sterilize.

Vitamin Solution: Composition per liter: Pyridoxine-HCl ........................................................................ 10.0mg Thiamine-HCl·2H2O .................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg D-Ca-pantothenate...................................................................... 5.0mg p-Aminobenzoic acid ................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize.

Trace Elements Solution: Composition per 200.0mL: MnCl2·4H2O................................................................................ 0.72g Fe(NH4)2(SO4)2·6H2O ................................................................. 0.4g FeSO4·7H2O.................................................................................. 0.2g CoCl2·6H2O .................................................................................. 0.2g ZnSO4·7H2O ................................................................................. 0.2g NiCl2·6H2O ................................................................................... 0.1g Na2MoO4·2H2O ......................................................................... 0.02g CuSO4·5H2O ............................................................................... 0.02g H3BO3 ......................................................................................... 0.02g KAl(SO4)2·12H2O....................................................................... 0.02g HCl.............................................................................................5.0mL

Tinsdale Agar Composition per 1100.0mL: Proteose peptone......................................................................... 20.0g Agar ............................................................................................ 15.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g L-Cystine ..................................................................................... 0.24g Tinsdale supplement ..............................................................150.0mL pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems and Oxoid Unipath.

Tinsdale Supplement: Composition per 100.0mL: Na2S2O3 ...................................................................................... 0.43g K2TeO3 ........................................................................................ 0.35g Serum.....................................................................................100.0mL

Caution: Potassium tellurite is toxic. Preparation of Tinsdale Supplement: Add Na2S2O3 and K2TeO3 to serum. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except Tinsdale supplement, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 100.0mL of sterile Tinsdale supplement. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the primary isolation and identification of Corynebacterium diphtheriae.

Tinsdale HiVeg Agar Base with Tinsdale Supplement Composition per liter: Plant peptone .............................................................................. 20.0g Agar ............................................................................................ 15.0g NaCl.............................................................................................. 5.0g Na2S2O3 ...................................................................................... 0.43g L-Cystine..................................................................................... 0.24g Tinsdale supplement ..............................................................150.0mL pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Preparation of Trace Elements: Add components to distilled/de-

Caution: Potassium tellurite is toxic.

ionized water and bring volume to 200.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Tinsdale Supplement: Add Na2S2O3 and K2TeO3

Preparation of Medium: Add components, except sulfide, bicarbonate, carbonate, and vitamin solutions, to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2 gas mixture. Dispense into culture vessels. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add sulfide, carbonate, bicarbonate, and vitamin solutions. Mix thoroughly. Adjust pH to 9.5.–10.0

Preparation of Medium: Add components, except Tinsdale sup-

to serum. Mix thoroughly. Filter sterilize.

plement, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 100.0mL of sterile Tinsdale supplement. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the primary isolation and identification of Corynebacterium diphtheriae.

Tissue Culture Amino Acids, Minimal Eagle 50X

Tissierella creatinophila Medium (DSMZ Medium 824) Composition per 1022.0mL: Creatine ......................................................................................... 3.8g Na-formate .................................................................................. 2.72g Yeast extract.................................................................................. 2.0g KCl................................................................................................ 1.0g MgSO4·7H2O ................................................................................ 0.5g NaCl .............................................................................................. 0.5g NH4Cl ......................................................................................... 0.25g KH2PO4 ......................................................................................... 0.2g CaCl2·2H2O................................................................................. 0.15g Resazurin ................................................................................... 0.5mg Na2SeO3·5H2O......................................................................... 0.26mg NaHCO3 solution .....................................................................40.0mL Na2S·9H2O solution .................................................................20.0mL Vitamin solution.......................................................................10.0mL Seven vitamin solution...............................................................1.0mL Trace elements solution SL-10 ..................................................1.0mL pH 7.5–7.8 ± 0.2 at 25°C Na2S·9H2O Solution: Composition per 20.0mL: Na2S·9H2O .................................................................................... 0.6g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature. NaHCO3 Solution: Composition per 100.0mL: NaHCO3 ...................................................................................... 10.0g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Filter sterilize.

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Preparation of Seven Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Sparge with 100% N2. Mix thoroughly. Filter sterilize. Trace Elements Solution SL-10: Composition per liter: FeCl2·4H2O................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O.............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly.

Preparation of Medium: Prepare and dispense medium under 100% N2. Add components, except NaHCO3 solution, Na2S·9H2O solution, seven vitamin solution, and vitamin solution, to 950.0mL distilled/deionized water. Mix thoroughly. Sparge with 100% N2 + 20% CO2. Adjust pH to 7.6. Dispense 10.0mL aliquots into bottles. Autoclave for 15 min at 15 psi pressure–121°C. For each 10.0mL medium aseptically and anaerobically inject from sterile stock solutions 0.4mL NaHCO3 solution, 0.2mL Na2S·9H2O solution, 0.1mL vitamin solution, and 0.01mL seven vitamin solution. Final pH should be 7.5–7.8.

Use: For the cultivation of Tissierella creatinophila.

Tissue Culture Amino Acids, HeLa 100X (TC Amino Acids, HeLa 100X) Composition per liter: L-Lysine..................................................................................... 0.029g L-Isoleucine ............................................................................... 0.026g

Vitamin Solution: Composition per liter:

L-Leucine

Pyridoxine-HCl ........................................................................ 10.0mg Thiamine-HCl·2H2O .................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg D-Ca-pantothenate...................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

L-Valine ..................................................................................... 0.023g

Preparation of Vitamin Solution: Add components to distilled/

Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2–7.4. Filter sterilize.

deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

.................................................................................. 0.026g

L-Threonine ............................................................................... 0.023g L-Tyrosine

................................................................................. 0.018g

L-Arginine ................................................................................. 0.017g L-Phenylalanine......................................................................... 0.016g L-Cystine ................................................................................... 0.012g L-Histidine.................................................................................. 7.8mg L-Methionine .............................................................................. 7.5mg L-Tryptophan.............................................................................. 4.1mg

pH 7.2–7.4 at 25°C

Preparation of Tissue Culture Amino Acids, HeLa 100X:

Use: For the preparation of Eagle HeLa medium for tissue culture procedures and virus studies.

Tissue Culture Amino Acids, Minimal Eagle 50X (TC Amino Acids, Minimal Eagle 50X) Composition per liter: L-Arginine ..................................................................................... 0.1g L-Lysine..................................................................................... 0.058g L-Isoleucine ............................................................................... 0.052g L-Leucine

.................................................................................. 0.052g

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Tissue Culture Dulbecco Solution

L-Threonine ............................................................................... 0.048g L-Valine ..................................................................................... 0.046g L-Tyrosine.................................................................................. 0.036g L-Phenylalanine......................................................................... 0.032g L-Histidine................................................................................. 0.031g L-Cystine ................................................................................... 0.024g L-Methionine ............................................................................. 0.015g L-Tryptophan ............................................................................... 0.01g

pH 7.2–7.4 at 25°C

Preparation of Tissue Culture Amino Acids, Minimal Eagle 50X: Add components to distilled/deionized water and bring volume

Na2HPO4 ..................................................................................... 0.06g KH2PO4....................................................................................... 0.06g Phenol Red.................................................................................. 0.02g pH 7.2–7.4 at 25°C

Source: This medium is available as a premixed solution from BD Diagnostic Systems.

Preparation of Tissue Culture Hanks Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2–7.4. Filter sterilize.

Use: For use in tissue culture procedures.

to 1.0L. Mix thoroughly. Adjust pH to 7.2–7.4. Filter sterilize.

Use: For the preparation of TC minimal medium Eagle for tissue culture procedures and virus studies.

Tissue Culture Medium 199 (TC Medium 199) Composition per 1050.0mL:

Tissue Culture Dulbecco Solution (TC Dulbecco Solution) Composition per liter: NaCl .............................................................................................. 8.0g Na2HPO4 ..................................................................................... 1.15g KH2PO4 ......................................................................................... 0.2g KCl................................................................................................ 0.2g CaCl2·2H2O................................................................................... 0.1g MgCl2·6H2O.................................................................................. 0.1g pH 7.2–7.4 at 25°C

Preparation of Tissue Culture Dulbecco Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2–7.4. Filter sterilize. Use: For use in tissue culture and virus preparations.

Tissue Culture Earle Solution (TC Earle Solution) Composition per 1002.0mL: NaCl .............................................................................................. 6.8g NaHCO3 ........................................................................................ 2.2g Glucose ......................................................................................... 1.0g KCl................................................................................................ 0.4g CaCl2·2H2O................................................................................... 0.2g NaH2PO4 ................................................................................... 0.125g MgSO4·7H2O ................................................................................ 0.1g Phenol Red (1% solution) ..........................................................2.0mL pH 7.2–7.4 at 25°C

Preparation of Tissue Culture Earle Solution: Add components, except Phenol Red, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Add 2.0mL of Phenol Red solution. Adjust pH to 7.2–7.4. Filter sterilize.

Use: For use in tissue culture and virus preparations.

Tissue Culture Hanks Solution (TC Hanks Solution) Composition per liter: NaCl .............................................................................................. 8.0g Glucose ......................................................................................... 1.0g KCl................................................................................................ 0.4g NaHCO3 ...................................................................................... 0.35g CaCl2·2H2O................................................................................. 0.14g MgCl2·6H2O.................................................................................. 0.1g MgSO4·7H2O ................................................................................ 0.1g © 2010 by Taylor and Francis Group, LLC

NaCl.............................................................................................. 8.0g Glucose ......................................................................................... 1.0g KCl................................................................................................ 0.4g NaHCO3 ...................................................................................... 0.35g DL-Glutamic acid......................................................................... 0.15g CaCl2·2H2O ................................................................................ 0.14g DL-Leucine .................................................................................. 0.12g L-Glutamine .................................................................................. 0.1g MgSO4·7H2O ................................................................................ 0.1g L-Arginine ................................................................................... 0.07g L-Lysine....................................................................................... 0.07g DL-Aspartic acid.......................................................................... 0.06g Na2HPO4 ..................................................................................... 0.06g KH2PO4....................................................................................... 0.06g DL-Threonine............................................................................... 0.06g DL-Alanine .................................................................................. 0.05g Glycine........................................................................................ 0.05g DL-Phenylalanine ........................................................................ 0.05g DL-Serine ..................................................................................... 0.05g Sodium acetate............................................................................ 0.05g DL-Valine ..................................................................................... 0.05g DL-Isoleucine............................................................................... 0.04g L-Proline...................................................................................... 0.04g L-Tyrosine ................................................................................... 0.04g DL-Methionine............................................................................. 0.03g L-Cystine ..................................................................................... 0.02g L-Histidine................................................................................... 0.02g Phenol Red.................................................................................. 0.02g DL-Tryptophan............................................................................. 0.02g Adenine....................................................................................... 0.01g L-Hydroxyproline........................................................................ 0.01g Tween™ 80................................................................................ 5.0mg Adenosine triphosphate ............................................................. 1.0mg Choline....................................................................................... 0.5mg Deoxyribose............................................................................... 0.5mg Ribose ........................................................................................ 0.5mg Guanine...................................................................................... 0.3mg Hypoxanthine............................................................................. 0.3mg Thymine..................................................................................... 0.3mg Uracil ......................................................................................... 0.3mg Xanthine..................................................................................... 0.3mg Adenylic acid ............................................................................. 0.2mg Cholesterol................................................................................. 0.2mg Calciferol ................................................................................... 0.1mg Fe(NO3)3·9H2O.......................................................................... 0.1mg L-Cysteine .................................................................................. 0.1mg

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Tissue Culture Medium Eagle, HeLa

Glutamine Solution: Composition per 100.0mL:

serum, bovine serum, horse serum, or fetal calf serum may be used. Mix thoroughly.

L-Glutamine................................................................................... 5.0g

Use: For the cultivation and maintenance of HeLa and other cell lines

NaCl (0.85% solution) ...........................................................100.0mL

in tissue culture, and for studying the cytopathogenicity of viral agents.

Preparation of Glutamine Solution: Add the glutamine to the 0.85% NaCl solution. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except glutamine and serum, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2–7.4. Filter sterilize. Aseptically add 6.0mL of sterile glutamine solution and 50.0–100.0mL of sterile serum. Human serum, bovine serum, horse serum, or fetal calf serum may be used. Mix thoroughly.

Use: For use as a base in the preparation of liquid media used for the cultivation of tissue culture cell lines.

Tissue Culture Medium Eagle, HeLa (TC Medium Eagle, HeLa) Composition per 1056.0mL: NaCl ............................................................................................ 5.85g NaHCO3 ...................................................................................... 1.68g Glucose ......................................................................................... 0.9g KCl............................................................................................ 0.373g NaH2PO4 ................................................................................... 0.138g MgCl2·6H2O................................................................................ 0.12g CaCl2·2H2O................................................................................. 0.11g L-Lysine................................................................................... 0.0269g L-Isoleucine ............................................................................. 0.0262g L-Leucine................................................................................. 0.0262g L-Threonine ............................................................................. 0.0238g L-Valine ................................................................................... 0.0234g L-Tyrosine................................................................................ 0.0181g L-Arginine ............................................................................... 0.0174g L-Phenylalanine....................................................................... 0.0165g L-Cystine ................................................................................... 0.012g L-Histidine.................................................................................. 7.8mg L-Methionine .............................................................................. 7.5mg Phenol Red ................................................................................. 5.0mg L-Tryptophan .............................................................................. 4.1mg Folic acid.................................................................................. 0.44mg Thiamine·HCl .......................................................................... 0.34mg Biotin ....................................................................................... 0.24mg Pantothenic acid ....................................................................... 0.22mg Pyridoxal·HCl ............................................................................ 0.2mg Choline chloride....................................................................... 0.14mg Nicotinamide............................................................................ 0.12mg Riboflavin ................................................................................ 0.04mg Serum ...................................................................... 50.0mL–100.0mL Glutamine solution.....................................................................6.0mL pH 7.2–7.4 at 25°C

Glutamine Solution: Composition per 100.0mL: L-Glutamine................................................................................... 5.0g

NaCl (0.85% solution) ...........................................................100.0mL

Preparation of Glutamine Solution: Add the glutamine to the

Tissue Culture Medium Ham F10 (TC Medium Ham F10) Composition per 1050.0mL: NaCl.............................................................................................. 7.4g Glucose ......................................................................................... 1.1g Na2HPO4 ..................................................................................... 0.29g KCl............................................................................................ 0.285g L-Arginine ................................................................................. 0.211g MgSO4·7H2O ............................................................................ 0.153g L-Glutamine ............................................................................ 0.1462g Sodium pyruvate......................................................................... 0.11g KH2PO4..................................................................................... 0.083g CaCl2·2H2O .............................................................................. 0.044g L-Cystine ................................................................................. 0.0315g L-Lysine................................................................................... 0.0293g L-Histidine................................................................................. 0.021g L-Asparagine ............................................................................. 0.015g L-Glutamic acid....................................................................... 0.0147g L-Aspartic acid ........................................................................ 0.0133g L-Leucine ................................................................................ 0.0131g L-Proline.................................................................................. 0.0115g L-Serine ................................................................................... 0.0105g L-Alanine.................................................................................. 8.91mg Glycine..................................................................................... 7.51mg L-Phenylalanine........................................................................ 4.96mg L-Methionine ............................................................................ 4.48mg Hypoxanthine............................................................................. 4.0mg L-Threonine .............................................................................. 3.57mg L-Valine ...................................................................................... 3.5mg L-Isoleucine ................................................................................ 2.6mg L-Tyrosine ................................................................................ 1.81mg Cyanocobalamin ........................................................................ 1.3mg Folic acid ................................................................................... 1.3mg Phenol Red................................................................................. 1.2mg Thiamine·HCl ............................................................................ 1.0mg FeSO4·7H2O............................................................................. 0.83mg Calcium pantothenate ................................................................ 0.7mg Thymidine.................................................................................. 0.7mg Choline chloride....................................................................... 0.69mg Niacinamide............................................................................... 0.6mg L-Tryptophan.............................................................................. 0.6mg i-Inositol................................................................................... 0.54mg Riboflavin ................................................................................ 0.37mg Lipoic acid ................................................................................. 0.2mg Pyridoxine·HCl .......................................................................... 0.2mg ZnSO4·7H2O .......................................................................... 0.028mg Biotin ..................................................................................... 0.024mg CuSO4·5H2O ............................................................................... 2.5μg Fetal calf serum.............................................................50.0–100.0mL pH 7.2–7.4 at 25°C

0.85% NaCl solution. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except fetal calf serum,

Preparation of Medium: Add components, except glutamine and

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2–7.4 with 10% Na2CO3 solution. Filter sterilize. Aseptically add 50.0–100.0mL of sterile fetal calf serum. Mix thoroughly.

serum, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2–7.4. Filter sterilize. Aseptically add 6.0mL of sterile glutamine solution and 50.0–100.0mL of sterile serum. Human © 2010 by Taylor and Francis Group, LLC

Use: For the cultivation of a wide variety of cell lines in tissue culture.

Tissue Culture Medium RPMI No. 1640

Tissue Culture Medium NCTC 109 (TC Medium NCTC 109) Composition per 1050.0mL: NaCl .............................................................................................. 6.8g NaHCO3 ........................................................................................ 2.2g Glucose ......................................................................................... 1.0g KCl................................................................................................ 0.4g L-Cysteine.................................................................................... 0.26g CaCl2·2H2O................................................................................... 0.2g NaH2PO4 ..................................................................................... 0.14g L-Glutamine................................................................................. 0.14g MgSO4·7H2O ................................................................................ 0.1g Sodium acetate ............................................................................ 0.05g Ascorbic acid .............................................................................. 0.05g L-Alanine..................................................................................... 0.03g L-Lysine....................................................................................... 0.03g L-Arginine ................................................................................. 0.026g L-Valine ..................................................................................... 0.025g L-Leucine..................................................................................... 0.02g Phenol Red .................................................................................. 0.02g L-Histidine................................................................................. 0.019g L-Threonine ............................................................................... 0.019g L-Isoleucine ............................................................................... 0.018g L-Tryptophan ............................................................................ 0.017.g L-Phenylalanine......................................................................... 0.017g L-Tyrosine.................................................................................. 0.016g Glycine...................................................................................... 0.014g Tween™ 80 ............................................................................... 0.012g L-Serine ..................................................................................... 0.011g L-Cystine ..................................................................................... 0.01g Glutathione.................................................................................. 0.01g Cyanocobalamin ......................................................................... 0.01g Deoxycytidine ............................................................................. 0.01g Deoxyguanosine.......................................................................... 0.01g Deoxyadenosine.......................................................................... 0.01g Thymidine ................................................................................... 0.01g L-Aspartic acid ......................................................................... 9.91mg L-Glutamic acid ........................................................................ 8.26mg L-Arginine ................................................................................ 8.09mg L-Ornithine ............................................................................... 7.38mg Nicotinamide adenine dinucleotide ........................................... 7.0mg L-Proline ................................................................................... 6.13mg L-α-N-butyric acid ................................................................... 5.51mg L-Methionine ............................................................................ 4.44mg L-Taurine .................................................................................. 4.18mg L-Hydroxyproline ..................................................................... 4.09mg D-Glucosamine ........................................................................... 3.2mg Coenzyme A .............................................................................. 2.5mg Glucuronolactone....................................................................... 1.8mg Sodium glucuronate ................................................................... 1.8mg Choline chloride....................................................................... 1.25mg Cocarboxylase............................................................................ 1.0mg Flavin adenine dinucleotide ....................................................... 1.0mg Uridine triphosphate .................................................................. 1.0mg Nicotinamide adenine dinucleotide phosphate ........................................................ 1.0mg Vitamin A................................................................................. 0.25mg Calciferol ................................................................................. 0.25mg i-Inositol................................................................................. 0.125mg p-Aminobenzoic acid............................................................. 0.125mg 5-Methylcytosine ....................................................................... 0.1mg Pyridoxine·HCl .................................................................... 0.0625mg © 2010 by Taylor and Francis Group, LLC

1793

Pyridoxal·HCl ...................................................................... 0.0625mg Niacin................................................................................... 0.0625mg Niacinamide......................................................................... 0.0625mg Biotin ..................................................................................... 0.025mg Folic acid ............................................................................... 0.025mg Menadione ............................................................................. 0.025mg Pantothenate........................................................................... 0.025mg Riboflavin .............................................................................. 0.025mg Thiamine·HCl ........................................................................ 0.025mg α-Tocopherol phosphate ........................................................ 0.025mg Serum............................................................................50.0–100.0mL pH 7.2–7.4 at 25°C

Preparation of Medium: Add components, except serum, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2–7.4 with 10% Na2CO3 solution. Filter sterilize. Aseptically add 50.0–100.0mL of sterile serum. Human serum, bovine serum, horse serum, or fetal calf serum may be used. Mix thoroughly. Use: For the cultivation of a wide variety of cell lines in tissue culture.

Tissue Culture Medium RPMI No. 1640 (TC Medium RPMI #1640) Composition per liter: NaCl............................................................................................ 6.46g Glucose ......................................................................................... 2.0g NaHCO3 ........................................................................................ 2.0g NaH2PO4 ................................................................................... 1.512g KCl................................................................................................ 0.4g L-Glutamine .................................................................................. 0.3g L-Arginine ..................................................................................... 0.2g Calcium nitrate.............................................................................. 0.1g MgSO4·7H2O ................................................................................ 0.1g L-Asparagine ............................................................................... 0.05g L-Cystine ..................................................................................... 0.05g L-Isoleucine ................................................................................. 0.05g L-Leucine .................................................................................... 0.05g L-Lysine·HCl ............................................................................... 0.04g Inositol ...................................................................................... 0.035g L-Serine ....................................................................................... 0.03g Hydroxy-L-proline ...................................................................... 0.02g L-Aspartic acid ............................................................................ 0.02g L-Glutamic acid........................................................................... 0.02g L-Proline...................................................................................... 0.02g L-Threonine ................................................................................. 0.02g L-Tyrosine ................................................................................... 0.02g L-Valine ....................................................................................... 0.02g L-Histidine................................................................................. 0.015g L-Methionine ............................................................................. 0.015g L-Phenylalanine......................................................................... 0.015g Glycine........................................................................................ 0.01g L-Tryptophan.............................................................................. 5.0mg Phenol Red................................................................................ 5.0mg Choline chloride......................................................................... 3.0mg p-Aminobenzoic acid................................................................. 1.0mg Folic acid ................................................................................... 1.0mg Glutathione ................................................................................ 1.0mg Nicotinamide.............................................................................. 1.0mg Pyridoxine·HCl .......................................................................... 1.0mg Thiamine·HCl ............................................................................ 1.0mg Calcium pantothenate .............................................................. 0.25mg Biotin ......................................................................................... 0.2mg

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Tissue Culture Minimal Medium Eagle

Riboflavin .................................................................................. 0.2mg Vitamin B12 ................................................................................ 5.0mg Serum ............................................................................50.0–100.0mL pH 7.2–7.4 at 25°C

Source: This medium is available as a premixed powder and solution from BD Diagnostic Systems.

Use: For the cultivation of a wide variety of cell lines in tissue culture.

Tissue Culture Minimal Medium Eagle Composition per liter: Sterile salt solution.................................................................944.0mL TC amino acids, minimal Eagle 50X.......................................20.0mL TC NaHCO3, 10%....................................................................20.0mL TC vitamins, minimal Eagle 100X ..........................................10.0mL TC glutamine, 5% ......................................................................6.0mL pH 7.2–7.4 at 25°C

Sterile Salt Solution: Composition per 944.0mL:

TC Vitamins, Minimal Eagle 100X: Composition per liter: Inositol ....................................................................................... 2.0mg Calcium pantothenate ................................................................ 1.0mg Choline chloride......................................................................... 1.0mg Folic acid ................................................................................... 1.0mg Nicotinamide.............................................................................. 1.0mg Pyridoxal.................................................................................... 1.0mg Thiamine·HCl ............................................................................ 1.0mg Riboflavin .................................................................................. 0.1mg

Preparation of TC Vitamins, Minimal Eagle 100X: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. TC Glutamine, 5%: Composition per 100.0mL: L-Glutamine

.................................................................................. 5.0g NaCl (0.85% solution) ...........................................................100.0mL

Preparation of TC Glutamine, 5%: Add the glutamine to the 0.85% NaCl solution. Mix thoroughly. Filter sterilize.

Preparation of Medium: Aseptically combine 944.0mL of sterile salt solution, 20.0mL of sterile TC amino acids, minimal Eagle 50X, 20.0mL of sterile TC NaHCO3, 10%, 10.0mL of sterile TC vitamins, minimal Eagle 100X, and 6.0mL of sterile TC glutamine, 5%. Mix thoroughly. Adjust pH to 7.2–7.4 if necessary.

NaCl .............................................................................................. 6.8g Glucose ......................................................................................... 1.0g KCl................................................................................................ 0.4g CaCl2 ............................................................................................. 0.2g MgCl2 ............................................................................................ 0.2g NaH2PO4 ..................................................................................... 0.15g

Use: For the cultivation of mammalian cells in monolayer or suspension for tissue culture procedures and virus preparation.

Preparation of Sterile Salt Solution: Add components to dis-

Composition per 1056.0mL:

tilled/deionized water and bring volume to 944.0mL. Mix thoroughly. Filter sterilize.

TC Amino Acids, Minimal Eagle 50X: Composition per liter: L-Arginine ..................................................................................... 0.1g L-Lysine....................................................................................... 0.06g L-Isoleucine ................................................................................. 0.05g L-Leucine..................................................................................... 0.05g L-Threonine ................................................................................. 0.05g L-Valine ....................................................................................... 0.05g L-Tyrosine.................................................................................... 0.04g L-Phenylalanine........................................................................... 0.03g L-Histidine................................................................................... 0.03g L-Cystine ..................................................................................... 0.02g L-Methionine ............................................................................... 0.02g L-Tryptophan ............................................................................... 0.01g

Preparation of TC Amino Acids, Minimal Eagle 50X: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2–7.4. Filter sterilize. TC NaHCO3, 10%: Composition per 100.0mL: NaHCO3 ...................................................................................... 10.0g

Tissue Culture Minimal Medium Eagle with Earle Balanced Salts Solution (TC Minimal Medium Eagle with Earle BSS) NaCl.............................................................................................. 6.8g Glucose ......................................................................................... 1.0g KCl................................................................................................ 0.4g CaCl2·2H2O .................................................................................. 0.2g MgCl2·6H2O ................................................................................. 0.2g NaH2PO4 ..................................................................................... 0.15g L-Arginine ..................................................................................... 0.1g L-Lysine....................................................................................... 0.06g L-Isoleucine ................................................................................. 0.05g L-Leucine .................................................................................... 0.05g L-Threonine ................................................................................. 0.05g L-Valine ....................................................................................... 0.05g L-Tyrosine ................................................................................... 0.04g L-Phenylalanine........................................................................... 0.03g L-Histidine................................................................................... 0.03g L-Cystine ..................................................................................... 0.02g L-Methionine ............................................................................... 0.02g L-Tryptophan............................................................................... 0.01g i-Inositol..................................................................................... 2.0mg Calcium pantothenate ................................................................ 1.0mg Choline chloride......................................................................... 1.0mg Folic acid ................................................................................... 1.0mg Nicotinamide.............................................................................. 1.0mg Pyridoxal.................................................................................... 1.0mg Thiamine·HCl ............................................................................ 1.0mg Riboflavin .................................................................................. 0.1mg Serum............................................................................50.0–100.0mL

TMA Mineral Medium

Glutamine solution.....................................................................6.0mL CaCl2·2H2O solution (optional) .................................................2.0mL pH 7.2–7.4 at 25°C

Glutamine Solution: Composition per 100.0mL:

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Glutamine Solution: Composition per 100.0mL: L-Glutamine

.................................................................................. 5.0g NaCl (0.85% solution) ...........................................................100.0mL

Preparation of Glutamine Solution: Add the glutamine to the

L-Glutamine................................................................................... 5.0g

0.85% NaCl solution. Mix thoroughly. Filter sterilize.

NaCl (0.85% solution) ...........................................................100.0mL

Preparation of Medium: Add components, except glutamine and

Preparation of Glutamine Solution: Add the glutamine to the

serum, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2–7.4 with 10% Na2CO3 solution. Filter sterilize. Aseptically add 6.0mL of sterile glutamine solution and 50.0– 100.0mL of sterile serum. Human serum, bovine serum, horse serum, or fetal calf serum may be used. Mix thoroughly.

0.85% NaCl solution. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except glutamine and serum, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2–7.4 with 10% Na2CO3 solution. Filter sterilize. Aseptically add 6.0mL of sterile glutamine solution and 50.0– 100.0mL of sterile serum. Human serum, bovine serum, horse serum, or fetal calf serum may be used. Mix thoroughly. To grow cells in a monolayer, aseptically add 2.0mL of a sterile 10% CaCl2·2H2O solution. To grow cells in suspension, omit the CaCl2·2H2O solution.

Use: For preparation of Eagle’s minimal medium for the cultivation of cells in monolayer or suspension in tissue culture.

Tissue Culture Minimal Medium Eagle Spinner Modified (TC Minimal Medium Eagle Spinner Modified MEM-S) Composition per 1056.0mL: NaH2PO4 ..................................................................................... 1.35g NaCl .............................................................................................. 6.8g NaHCO3 ........................................................................................ 2.2g Glucose ......................................................................................... 1.0g KCl................................................................................................ 0.4g CaCl2·2H2O................................................................................... 0.2g NaH2PO4 ................................................................................... 0.125g MgSO4·7H2O ................................................................................ 0.1g L-Isoleucine ............................................................................... 0.026g L-Leucine................................................................................... 0.026g L-Lysine..................................................................................... 0.026g L-Threonine ............................................................................... 0.024g L-Valine ................................................................................... 0.0235g L-Tyrosine.................................................................................. 0.018g L-Arginine ............................................................................... 0.0174g L-Phenylalanine....................................................................... 0.0165g L-Cystine ................................................................................... 0.012g L-Histidine.................................................................................. 8.0mg L-Methionine .............................................................................. 7.5mg Phenol Red ................................................................................. 5.0mg L-Tryptophan .............................................................................. 4.0mg Inositol ....................................................................................... 1.8mg Biotin ......................................................................................... 1.0mg Calcium pantothenate ................................................................ 1.0mg Choline chloride......................................................................... 1.0mg Folic acid.................................................................................... 1.0mg Nicotinamide.............................................................................. 1.0mg Pyridoxal·HCl ............................................................................ 1.0mg Thiamine·HCl ............................................................................ 1.0mg Riboflavin .................................................................................. 0.1mg Serum ...................................................................... 50.0mL–100.0mL Glutamine solution.....................................................................6.0mL pH 7.2–7.4 at 25°C © 2010 by Taylor and Francis Group, LLC

Use: For the cultivation of mammalian cells in suspension.

Tissue Culture Tyrode Solution (TC Tyrode Solution) Composition per 1002.0mL: NaCl.............................................................................................. 8.0g Glucose ......................................................................................... 1.0g NaHCO3 ........................................................................................ 1.0g CaCl2·2H2O .................................................................................. 0.2g KCl................................................................................................ 0.2g MgCl2·6H2O ................................................................................. 0.1g NaH2PO4 ..................................................................................... 0.05g Phenol Red (1% solution)..........................................................2.0mL pH 7.2–7.4 at 25°C

Preparation of Tissue Culture Tyrode Solution: Add components, except Phenol Red, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Add 2.0mL of Phenol Red solution. Adjust pH to 7.2–7.4. Filter sterilize. Use: For use in tissue culture procedures.

Tissue Culture Vitamins Minimal Eagle, 100X (TC Vitamins Minimal Eagle, 100X) Composition per liter: Inositol ....................................................................................... 2.0mg Calcium pantothenate ................................................................ 1.0mg Choline chloride......................................................................... 1.0mg Folic acid ................................................................................... 1.0mg Nicotinamide.............................................................................. 1.0mg Pyridoxal.................................................................................... 1.0mg Thiamine·HCl ............................................................................ 1.0mg Riboflavin .................................................................................. 0.1mg pH 7.2–7.4 at 25°C

Preparation of TC Vitamins, Minimal Eagle 100X: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Use: For the preparation of Tissue Culture minimal medium Eagle used in tissue culture procedures.

TMA Mineral Medium Composition per liter: Agar, noble.................................................................................. 20.0g KH2PO4....................................................................................... 2.78g Na2HPO4 ..................................................................................... 2.78g (NH4)2SO4 .................................................................................... 1.0g

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TMAO HiVeg Medium

Tetramethylammonium perchlorate solution ...........................20.0mL Hutner's basal salts solution.....................................................20.0mL pH 6.8 ± 0.2 at 25°C

Tetramethylammonium Perchlorate Solution: Composition per 20.0mL: Tetramethylammonium perchlorate .............................................. 1.0g

Preparation of Tetramethylammonium Perchlorate Solution: Add tetramethylammonium perchlorate to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize.

Hutner’s Basal Salts Solution: Composition per liter: MgSO4·7H2O .............................................................................. 29.7g Nitrilotriacetic acid ..................................................................... 10.0g CaCl2·2H2O............................................................................... 3.335g FeSO4·7H2O............................................................................. 99.0mg (NH4)6MoO7O24·4H2O ............................................................ 9.25mg "Metals 44" ..............................................................................50.0mL

"Metals 44": Composition per 100.0mL: ZnSO4·7H2O ............................................................................. 1.095g FeSO4·7H2O.................................................................................. 0.5g Sodium EDTA............................................................................. 0.25g MnSO4·H2O.............................................................................. 0.154g CuSO4·5H2O ............................................................................ 39.2mg Co(NO3)2·6H2O ....................................................................... 24.8mg Na2B4O7·10H2O....................................................................... 17.7mg

Preparation of “Metals 44”: Add sodium EDTA to distilled/deionized water and bring volume to 90.0mL. Mix thoroughly. Add a few drops of concentrated H2SO4 to retard precipitation of heavy metal ions. Add remaining components. Mix thoroughly. Bring volume to 100.0mL with distilled/deionized water. Preparation of Hutner’s Basal Salts Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Adjust pH to 6.8.

Preparation of Medium: Add components, except tetramethylammonium perchlorate solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 6.8. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 20.0mL of sterile tetramethylammonium perchlorate solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Paracoccus kocurii.

TMAO HiVeg Medium (Trimethylamine-N-Oxide HiVeg Medium) Composition per liter: Plant extract ................................................................................ 10.0g Plant peptone............................................................................... 10.0g NaCl .............................................................................................. 5.0g Agar .............................................................................................. 2.0g Trimethylamine-N-oxide............................................................... 1.0g Yeast extract.................................................................................. 1.0g pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into screw-capped tubes in 4.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to cool in an upright position.

Use: For the cultivation and differentiation of Campylobacter species from foods. Campylobacter jejuni and Campylobacter coli will not grow.

TMAO Medium See: Trimethylamine N-Oxide Medium

TMBS4 Medium (DSMZ Medium 559) Composition per 1003.0mL: Solution A..............................................................................870.0mL Solution C ..............................................................................100.0mL Solution D................................................................................10.0mL Solution E (Vitamin solution)..................................................10.0mL Solution F.................................................................................10.0mL Solution B (Trace elements solution SL-10) .............................1.0mL Solution G..................................................................................1.0mL Solution H..................................................................................1.0mL pH 7.1–7.4 at 25°C

Solution A: Composition per 870.0mL: NaCl.............................................................................................. 1.0g MgCl2·6H2O ................................................................................. 0.4g Na2SO4 .......................................................................................... 3.0g KCl................................................................................................ 0.5g NH4Cl ........................................................................................... 0.3g KH2PO4......................................................................................... 0.2g CaCl2·2H2O ................................................................................ 0.15g Resazurin ................................................................................... 1.0mg

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 870.0mL Mix thoroughly.

Solution B (Trace Elements Solution SL-10): Composition per liter: FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Solution C: Composition per 100.0mL: NaHCO3 ........................................................................................ 5.0g

Preparation of Solution C: Add NaHCO3 to distilled/deionized

water and bring volume to 100.0mL Mix thoroughly. Filter sterilize. Flush with 80% N2 + 20% CO2 to remove dissolved oxygen.

TMBS4 Medium

Solution D: Composition per 10.0mL: Syringic acid ................................................................................. 0.6g

Preparation of Solution D: Add syringic acid to distilled/deionized water and bring volume to 10.0mL. Adjust pH to 8.0 with NaOH. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Solution E (Vitamin Solution): Composition per liter: Pyridoxine-HCl ........................................................................ 10.0mg Thiamine-HCl·2H2O .................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg D-Ca-pantothenate...................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 .............................................................................. 0.10mg

Solution E (Vitamin Solution): Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Solution F: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.4g

Preparation of Solution F: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Solution G: Composition per 10.0mL: Na-dithionite ............................................................................... 0.25g

Preparation of Solution G: Add Na-dithionite to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Solution H: Composition per liter:

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Solution E (Vitamin solution)..................................................10.0mL Solution F.................................................................................10.0mL Solution B (Trace elements solution SL-10) .............................1.0mL Solution G..................................................................................1.0mL pH 7.1–7.4 at 25°C

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 870.0mL. Mix thoroughly. Gently heat and bring to boiling. Continue boiling for 3–4 min. Allow to cool to room temperature while gassing under 80% N2 + 20% CO2. Continue gassing until pH reaches below 6.0. Seal the flask under 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C.

Solution B (Trace Elements Solution SL-10): Composition per liter: FeCl2·4H2O................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O.............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Preparation of Solution B (Trace Elements Solution SL-10): Add FeCl2·4H2O to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Gas under 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Solution C: Composition per 100.0mL:

Preparation of Solution H: Add components to distilled/deionized

Preparation of Solution C: Add NaHCO3 to distilled/deionized

NaHCO3 ........................................................................................ 5.0g

water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Gas under 80% N2 + 20% CO2.

Preparation of Medium: Gently heat solution A and bring to boil-

Solution D: Composition per 10.0mL:

ing. Boil solution A for a few minutes. Cool to room temperature. Gas with 80% N2 + 20% CO2 gas mixture to reach a pH below 6. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Sequentially add 1.0mL solution B, 100.0mL solution C, 10.0mL solution D, 10.0mL solution E, 10.0mL solution F, 1.0mL solution G, and 1.0mL solution H. Distribute anaerobically under 80% N2 + 20% CO2 into appropriate vessels.

Use: For the cultivation of Holophaga foetida.

TMBS4 Medium Composition per 1002.0mL: Solution A ..............................................................................870.0mL Solution C ..............................................................................100.0mL Solution D ................................................................................10.0mL © 2010 by Taylor and Francis Group, LLC

Syringic acid ................................................................................. 0.5g

Preparation of Solution D: Add syringic acid to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Adjust pH to 8 with NaOH. Gas under 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Solution E (Vitamin Solution): Composition per liter: Pyridoxine·HCl ........................................................................ 10.0mg Calcium DL-pantothenate........................................................... 5.0mg Lipoic acid ................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg

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T1N0 Broth

Thiamine·HCl ............................................................................ 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

T1N1 Broth (Tryptone Salt Broth) Composition per liter: NaCl............................................................................................ 10.0g Pancreatic digest of casein.......................................................... 10.0g pH 7.1 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

Solution F: Composition per 10.0mL:

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Na2S·9H2O .................................................................................. 0.25g Na2S2O4 ...................................................................................... 0.25g

Use: For the cultivation of Vibrio cholerae and other Vibrio species.

Preparation of Solution F: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Gas under 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

T1N2 Agar (Tryptone Salt Agar)

Solution G: Composition per liter: NaOH ............................................................................................ 0.5g Na2WO4·2H2O ........................................................................... 4.0mg Na2SeO3·5H2O........................................................................... 3.0mg

Preparation of Solution G: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gas under 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Aseptically and anaerobically combine solution A with solution B, solution C, solution D, solution E, solution F, and solution G, in that order. Mix thoroughly. Anaerobically distribute into sterile tubes or flasks under 80% N2 + 20% CO2.

Use: For the cultivation and maintenance of Pelobacter species. TN Broth See: Trypticase™ Novobiocin Broth

T1N0 Broth (Tryptone Broth) Composition per liter: Pancreatic digest of casein .......................................................... 10.0g pH 7.1 ± 0.2 at 25°C

Preparation of Medium: Add pancreatic digest of casein to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation of Vibrio cholerae and other Vibrio species.

T1N1 Agar (Tryptone Salt Agar) Composition per liter: Agar ............................................................................................ 20.0g NaCl ............................................................................................ 10.0g Pancreatic digest of casein .......................................................... 10.0g pH 7.1 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Allow tubes to cool in a slanted position.

Composition per liter: Agar ............................................................................................ 20.0g NaCl............................................................................................ 20.0g Pancreatic digest of casein.......................................................... 10.0g pH 7.1 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Allow tubes to cool in a slanted position.

Use: For the cultivation of Vibrio cholerae and other Vibrio species.

T1N3 Broth (Tryptone Salt Broth) Composition per liter: NaCl............................................................................................ 30.0g Pancreatic digest of casein.......................................................... 10.0g pH 7.1 ± 0.2 at 25°C

Use: For the cultivation of Vibrio cholerae and other Vibrio species.

T1N6 Broth (Tryptone Salt Broth) Composition per liter: NaCl............................................................................................ 60.0g Pancreatic digest of casein.......................................................... 10.0g pH 7.1 ± 0.2 at 25°C

Use: For the cultivation of Vibrio cholerae and other Vibrio species.

T1N8 Broth (Tryptone Salt Broth) Composition per liter: NaCl............................................................................................ 80.0g Pancreatic digest of casein.......................................................... 10.0g pH 7.1 ± 0.2 at 25°C

Todd-Hewitt Broth

1799

Preparation of Medium: Add components to distilled/deionized

Source: This medium is available as a prepared medium from BD Di-

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

agnostic Systems.

Use: For the cultivation of Vibrio cholerae and other Vibrio species.

T1N10 Broth (Tryptone Salt Broth) Composition per liter: NaCl .......................................................................................... 100.0g Pancreatic digest of casein .......................................................... 10.0g pH 7.1 ± 0.2 at 25°C

Use: For the cultivation of Vibrio cholerae and other Vibrio species.

TN HiVeg Agar

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes.

Use: For the differentiation and identification of Candida albicans and Cryptococcus neoformans. Cryptococcus albicans produces germ tubes and chlamydospores when grown on this medium. Cryptococcus neoformans appears as tan to brown colonies.

Todd-Hewitt Broth Composition per liter: Beef heart, infusion from.......................................................... 500.0g Neopeptone ................................................................................. 20.0g Na2CO3 ......................................................................................... 2.5g Glucose ......................................................................................... 2.0g NaCl.............................................................................................. 2.0g Na2HPO4 ....................................................................................... 0.4g pH 7.8 ± 0.2 at 25°C

Composition per liter:

Source: This medium is available as a premixed powder from BD Di-

agnostic Systems.

Source: This medium is available as a premixed powder from HiMedia.

Use: For the isolation and cultivation of vibrios from food samples. TNSA Agar See: Trypaflavin Nalidixic Acid Serum Agar

TNT Medium (Tryptone NaCl Thiamine Medium) Composition per liter: Pancreatic digest of casein .......................................................... 10.0g NaCl .............................................................................................. 5.0g Thiamine·HCl ............................................................................ 1.0mg pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Escherichia coli.

TOC Agar (Tween™ 80 Oxgall Caffeic Acid Agar) Composition per liter: Agar ............................................................................................ 20.0g Oxgall.......................................................................................... 10.0g Caffeic acid ................................................................................... 0.3g Tween™ 80 ..............................................................................10.0mL © 2010 by Taylor and Francis Group, LLC

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of group A streptococci used in serological typing, and for the cultivation of a variety of pathogenic microorganisms.

Todd-Hewitt Broth Composition per liter: Pancreatic digest of casein.......................................................... 20.0g Infusion from 450.0g fat-free minced meat................................ 10.0g Glucose ......................................................................................... 2.0g NaHCO3 ........................................................................................ 2.0g NaCl.............................................................................................. 2.0g Na2HPO4 ....................................................................................... 0.4g pH 7.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 10 min at 10 psi pressure–115°C.

Use: For the cultivation of group A streptococci used in serological typing, and for the cultivation of a variety of pathogenic microorganisms.

Todd-Hewitt Broth (ATCC Medium 235) Composition per liter: Peptone ....................................................................................... 20.0g Beef heart, solids from infusion.................................................... 3.1g Na2CO3 ......................................................................................... 2.5g Glucose ......................................................................................... 2.0g NaCl.............................................................................................. 2.0g Na2HPO4 ....................................................................................... 0.4g pH 7.8 ± 0.2 at 25°C

1800

Todd-Hewitt Broth, Modified

Use: For the cultivation of group A streptococci used in serological typing, and for the cultivation of a variety of pathogenic microorganisms.

Todd-Hewitt Broth, Modified Composition per liter: Neopeptone ................................................................................. 20.0g Glucose ......................................................................................... 2.0g NaHCO3 ........................................................................................ 2.0g NaCl .............................................................................................. 2.0g Na2HPO4 ....................................................................................... 0.4g Beef heart infusion........................................................................1.0L pH 7.8 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 10 min at 10 psi pressure–115°C.

Use: For the cultivation of streptococci for serological identification.

Todd-Hewitt HiVeg Broth Composition per liter: Plant peptone............................................................................... 20.0g Plant special infusion .................................................................. 10.0g Na2CO3 ......................................................................................... 2.5g NaCl .............................................................................................. 2.0g Glucose ......................................................................................... 2.0g Na2HPO4 ....................................................................................... 0.4g pH 7.8 ± 0.2 at 25°C

Toluidine Blue DNA Agar Composition per liter: Agar ............................................................................................ 10.0g NaCl............................................................................................ 10.0g Tris(hydroxymethyl)aminomethane buffer................................... 6.1g Deoxyribonucleic acid .................................................................. 0.3g Toluidine Blue O....................................................................... 0.083g CaCl2, anhydrous ....................................................................... 1.1mg pH 9.0 ± 0.2 at 25°C

Preparation of Medium: Add tris(hydroxymethyl)aminomethane buffer to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 9.0. Add the remaining components, except Toluidine Blue O. Mix thoroughly. Gently heat and bring to boiling. Add Toluidine Blue O. Mix thoroughly. If used the same day, sterilization is not necessary. Cool to 50°C. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation and differentiation of Staphylococcus aureus from foods.

Toluidine Blue DNA Agar Composition per liter: Agar ............................................................................................ 10.0g NaCl............................................................................................ 10.0g Tris(hydroxymethyl)aminomethane buffer................................... 6.1g Deoxyribonucleic acid (DNA)...................................................... 0.3g Toluidine Blue O....................................................................... 0.083g CaCl2, anhydrous ....................................................................... 1.1mg pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except Toluidine Blue

Source: This medium is available as a premixed powder from Hi-

O, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Add Toluidine Blue O. Mix thoroughly. Medium does not have to be sterilized if used immediately. Pour into sterile Petri dishes or distribute into sterile tubes. Allow tubes to cool in a slanted position.

Media.

Use: For the cultivation and differentiation of bacteria based on their

Preparation of Medium: Add components to distilled/deionized

production of deoxyribonuclease (DNase). Bacteria that produce DNase turn the medium pink.

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–1°C.

Use: For the cultivation of group A streptococci used in serological typing, and for the cultivation of a variety of pathogenic microorganisms.

Todd-Hewitt Medium (DSMZ Medium 697) Composition per liter: Casein peptone ............................................................................ 20.0g Meat infusion .............................................................................. 10.0g Glucose ......................................................................................... 2.0g NaHCO3 ........................................................................................ 2.0g NaCl .............................................................................................. 2.0g Na2HPO4 ....................................................................................... 0.4g pH 7.8 ± 0.2 at 25°C

Tomato Dextrin Yeast Medium Composition per liter: Tomato paste ............................................................................... 20.0g Dextrin ........................................................................................ 20.0g Agar ............................................................................................ 20.0g Baker’s yeast............................................................................... 10.0g CoCl2·6H2O ............................................................................... 5.0mg pH 7.2–7.4 at 25°C

Preparation of Medium: Add components, except agar, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2–7.4. Add agar. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Streptomyces avermitilis.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Tomato Juice Agar Composition per liter: Agar ............................................................................................ 12.0g Pancreatic digest of casein.......................................................... 10.0g

Tomato Juice HiVeg Agar, Special

1801

Peptonized milk .......................................................................... 10.0g Tomato juice...........................................................................400.0mL pH 6.1 ± 0.2 at 25°C

MnSO4·7H2O .............................................................................. 0.01g NaCl............................................................................................ 0.01g pH 6.7 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di-

agnostic Systems and Oxoid Unipath.

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

Use: For the cultivation of yeast and other aciduric microorganisms.

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of lactobacilli, especially Lactobacillus acido-

Tomato Juice Broth (ATCC Medium 433)

philus.

Tomato Juice Agar (ATCC Medium 33) Composition per liter: Agar ............................................................................................ 11.0g Pancreatic digest of casein .......................................................... 10.0g Yeast extract................................................................................ 10.0g Tomato juice, filtered .............................................................200.0mL pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Composition per liter: Pancreatic digest of casein.......................................................... 10.0g Yeast extract................................................................................ 10.0g Tomato juice, filtered.............................................................200.0mL pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of a variety of fastidious bacteria that require complex growth factors, including Lactobacillus, Aerococcus, Bifidobacterium, and Pediococcus species.

Tomato Juice HiVeg Agar

Use: For the cultivation and maintenance of a variety of bacteria

Composition per liter:

including Lactobacillus, Leuconostoc, Pediococcus, and Propionibacterium species.

Agar ............................................................................................ 11.0g Plant hydrolysate ........................................................................ 10.0g Plant hydrolysate No. 3............................................................... 10.0g Tomato juice ..........................................................................400.0mL pH 5.0 ± 0.2 at 25°C

Tomato Juice Agar Special Composition per liter: Agar ............................................................................................ 20.0g Pancreatic digest of casein .......................................................... 10.0g Peptonized milk .......................................................................... 10.0g Tomato juice...........................................................................400.0mL pH 5.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Avoid overheating—it results in a soft agar. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and enumeration of lactobacilli.

Tomato Juice Broth

Source: This medium is available as a premixed powder from HiMedia.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Avoid overheating—it results in a soft agar. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and enumeration of lactobacilli.

Tomato Juice HiVeg Agar, Special Composition per liter: Agar ............................................................................................ 20.0g Plant hydrolysate No. 3............................................................... 10.0g Plant peptone .............................................................................. 10.0g Tomato juice ..........................................................................400.0mL pH 5.0 ± 0.2 at 25°C

Composition per liter:

Source: This medium is available as a premixed powder from Hi-

Tomato juice, dessicated ............................................................. 20.0g Glucose ....................................................................................... 10.0g Yeast extract................................................................................ 10.0g K2HPO4 ......................................................................................... 0.5g KH2PO4 ......................................................................................... 0.5g MgSO4·7H2O ................................................................................ 0.2g FeSO4·7H2O................................................................................ 0.01g

Media.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Avoid overheating—it results in a soft agar. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and enumeration of lactobacilli.

1802

Tomato Juice HiVeg Medium Base with Tomato Juice and Cycloheximide

Tomato Juice HiVeg Medium Base with Tomato Juice and Cycloheximide Composition per liter: Agar ............................................................................................ 15.0g Glucose ....................................................................................... 10.0g Tomato juice solids, from 150.0mL.............................................. 7.5g Yeast extract.................................................................................. 5.0g Plant special peptone .................................................................... 5.0g KH2PO4 ......................................................................................... 0.5g CaCl2 ......................................................................................... 0.125g MgSO4 ...................................................................................... 0.125g KCl............................................................................................ 0.125g NaCl .......................................................................................... 0.125g Bromcresol Green ....................................................................... 0.03g MnSO4 ........................................................................................ 0.03g Tomato juice, canned .............................................................150.0mL Cycloheximide solution ...........................................................10.0mL pH 5.0 ± 0.2 at 25°C

Cycloheximide Solution: Composition per 10.0mL: Cycloheximide .............................................................................. 0.1g

Preparation of Medium: Add components, except cycloheximide solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile cycloheximide solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the isolation and cultivation of lactobacilli from wine.

Tomato Juice Medium Composition per liter: Tryptic digest of casein............................................................... 10.0g Yeast extract................................................................................ 10.0g Tomato juice, filtered, pH 7.0 ................................................200.0mL Tween™ 80................................................................................1.0mL pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Lactobacillus collinoides.

Preparation of Cycloheximide Solution: Add cycloheximide to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Caution: Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation.

Preparation of Medium: Add components, except cycloheximide solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile cycloheximide solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the isolation and cultivation of lactobacilli.

Tomato Juice Medium Composition per liter: Agar ............................................................................................ 15.0g Glucose ....................................................................................... 10.0g Polypeptone™............................................................................... 5.0g Yeast extract.................................................................................. 5.0g KH2PO4 ......................................................................................... 0.5g CaCl2·2H2O............................................................................... 0.125g KCl............................................................................................ 0.125g MgSO4·7H2O ............................................................................ 0.125g NaCl .......................................................................................... 0.125g Bromcresol Green ....................................................................... 0.03g MnSO4·4H2O ............................................................................. 3.0mg Tomato juice, canned .............................................................150.0mL Cycloheximide solution ...........................................................10.0mL pH 5.0 ± 0.2 at 25°C

Cycloheximide Solution: Composition per 10.0mL: Cycloheximide .............................................................................. 0.1g

Preparation of Cycloheximide Solution: Add cycloheximide to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Tomato Juice Milk Agar (DSMZ Medium 353) Composition per liter: Skim milk.................................................................................. 100.0g Yeast extract.................................................................................. 5.0g Tomato juice ..........................................................................100.0mL pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Filter canned tomatoes through paper to produce tomato juice. Leave overnight at 10°C. Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of Enterococcus faecalis=Streptococcus faecalis.

Tomato Juice Yeast Extract Medium Composition per liter: Skim milk.................................................................................. 100.0g Yeast extract.................................................................................. 5.0g Tomato juice, filtered.............................................................100.0mL

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of yeasts and fungi.

Tomato Juice Yeast Extract Milk Medium Composition per liter: Skim milk.................................................................................. 100.0g Yeast extract.................................................................................. 5.0g Tomato juice, filtered.............................................................100.0mL pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Filter canned tomato juice through paper. Let stand overnight at 10°C. Add remaining components and bring to 1.0L with distilled/deionized water. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

TPEY Agar Use: For the cultivation and maintenance of a variety of fastidious bacteria that require complex growth factors, including Lactobacillus, Streptococcus, and Enterococcus species.

Tomato Paste Oatmeal Agar Composition per liter: Oatmeal (dried baby food) .......................................................... 20.0g Tomato paste ............................................................................... 20.0g Agar ............................................................................................ 15.0g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Melt agar by steaming for 20–30 min at 0 psi pressure–100°C. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Flexibacter species.

Top Agarose Composition per liter: Pancreatic digest of casein .......................................................... 10.0g NaCl .............................................................................................. 8.0g Agarose ......................................................................................... 6.0g pH 7.0 ± 0.2 at 25°C

1803

Glutamine ................................................................................. 0.292g Histidine.................................................................................... 0.031g Lysine........................................................................................ 0.058g Isoleucine.................................................................................. 0.052g Leucine ..................................................................................... 0.052g Phenol Red................................................................................ 0.050g Threonine.................................................................................. 0.048g Valine ........................................................................................ 0.046g Tyrosine .................................................................................... 0.036g Phenylalanine............................................................................ 0.032g Methionine................................................................................ 0.015g Tryptophan................................................................................ 0.010g Inositol ....................................................................................... 2.0mg Choline....................................................................................... 1.0mg Folic acid ................................................................................... 1.0mg Nicotinamide.............................................................................. 1.0mg Pantothenic acid......................................................................... 1.0mg Pyridoxal·HCl ............................................................................ 1.0mg Thiamine·HCl ............................................................................ 1.0mg Riboflavin .................................................................................. 0.1mg Fetal bovine serum, heat inactivated .....................................100.0mL pH 7.2–7.4 at 25°C

Preparation of Medium: Add components, except fetal bovine se-

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 25 min at 15 psi pressure–121°C.

rum, to distilled/deionized water and bring volume to 905.0mL. Mix thoroughly. Adjust pH to 7.2–7.4. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 100.0mL of sterile, heat-inactivated fetal bovine serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the distribution of bacteriophage or bacterial cells, especially

Use: For the cultivation of Toxoplasma gondii.

Preparation of Medium: Add components to distilled/deionized

Escherichia coli, evenly in a thin layer over the surface of a plate.

Torulopsis Medium Composition per liter: Glucose ..................................................................................... 100.0g Agar ............................................................................................ 20.0g Casamino acids ............................................................................. 4.0g Yeast extract.................................................................................. 1.0g KH2PO4 ......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g CaCl2·2H2O................................................................................... 0.1g NaCl .............................................................................................. 0.1g pH 5.6 ± 0.2 at 25°C

TPBY See: Tryptone Phosphate Brain Heart Infusion Yeast Extract Agar

TPEY Agar (Tellurite Polymyxin Egg Yolk Agar) Composition per liter:

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 5.6. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

NaCl............................................................................................ 20.0g Agar ............................................................................................ 15.5g Pancreatic digest of casein.......................................................... 10.0g Yeast extract.................................................................................. 5.0g D-Mannitol .................................................................................... 5.0g LiCl ............................................................................................... 2.0g Egg yolk emulsion (30% solution) ........................................100.0mL Chapman tellurite solution.......................................................10.0mL Polymyxin B solution ................................................................0.4mL pH 7.1 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Candida versatilis.

Source: This medium is available as a premixed powder from BD Di-

Preparation of Medium: Add components to distilled/deionized

agnostic Systems.

Toxoplasma Medium Composition per liter: NaCl .............................................................................................. 6.8g NaHCO3 ........................................................................................ 2.2g Glucose ......................................................................................... 1.0g KCl................................................................................................ 0.4g CaCl2 ............................................................................................. 0.2g NaH2PO4·H2O........................................................................... 0.125g Arginine .................................................................................... 0.105g MgSO4 .......................................................................................... 0.1g L-Cystine ................................................................................... 0.024g © 2010 by Taylor and Francis Group, LLC

Egg Yolk Emulsion (30% Solution): Composition per 100.0mL: NaCl.............................................................................................. 0.6g Egg yolk...................................................................................30.0mL

Preparation of Egg Yolk Emulsion (30% Solution): Add NaCl and egg yolk to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Chapman Tellurite Solution: Composition per 100.0mL: K2TeO3 .......................................................................................... 1.0g

1804

TPEY HiVeg Agar Base with Egg Yolk, Tellurite, and Polymyxin B

Preparation of Chapman Tellurite Solution: Add K2TeO3 to

distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Polymyxin B Solution: Composition per 100.0mL: Polymyxin B ................................................................................. 1.0g

Preparation of Polymyxin B Solution: Add polymyxin B to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Caution: Potassium tellurite is toxic. Preparation of Medium: Add components—except 30% egg yolk emulsion, Chapman tellurite solution, and polymyxin B solution—to distilled/deionized water and bring volume to 890.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of sterile 30% egg yolk emulsion, 10.0mL of sterile Chapman tellurite solution, and 0.4mL of sterile polymyxin B solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the recovery of staphylococci from foods and other materials.

TPEY HiVeg Agar Base with Egg Yolk, Tellurite, and Polymyxin B Composition per liter: NaCl ............................................................................................ 20.0g Agar ............................................................................................ 18.0g Plant hydrolysate......................................................................... 10.0g D-Mannitol .................................................................................... 5.0g Yeast extract.................................................................................. 5.0g LiCl ............................................................................................... 2.0g Egg yolk emulsion (30% solution) ........................................100.0mL Chapman tellurite solution.......................................................10.0mL Polymyxin B solution ................................................................0.4mL pH 7.1 ± 0.2 at 25°C

Source: This medium, without egg yolk, tellurite, and polymyxin B,

Preparation of Medium: Add components—except 30% egg yolk emulsion, Chapman tellurite solution, and polymyxin B solution—to distilled/deionized water and bring volume to 890.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of sterile 30% egg yolk emulsion, 10.0mL of sterile Chapman tellurite solution, and 0.4mL of sterile polymyxin B solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the recovery of staphylococci from foods and other materials. TPGY Broth See: Trypticase™ Peptone Glucose Yeast Extract Broth

TPGY Medium (Thioglycolate Peptone Glucose Yeast Extract Medium) Composition per liter: Pancreatic digest of casein.......................................................... 50.0g Peptone ......................................................................................... 5.0g Yeast extract.................................................................................. 5.0g Glucose ......................................................................................... 1.0g Sodium thioglycolate .................................................................... 1.0g pH 7.1 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of a variety of anaerobic bacteria. TPGYT Broth See: Trypticase™ Peptone Glucose Yeast Extract Broth with Trypsin

TPL Medium (Thioglycolate Potato Liver Medium)

is available as a premixed powder from HiMedia.

Composition per liter:

Egg Yolk Emulsion (30% Solution): Composition per 100.0mL:

Potato ........................................................................................ 200.0g Yeast extract................................................................................ 31.0g Liver............................................................................................ 25.0g Glycerol ...................................................................................... 15.0g Agar ............................................................................................ 15.0g Meat extract .................................................................................. 5.5g Glucose ......................................................................................... 7.5g Peptone ......................................................................................... 2.5g NaCl.............................................................................................. 2.5g Sodium thioglycolate .................................................................... 0.5g Methylene Blue.......................................................................... 1.0mg pH 7.0 ± 0.2 at 25°C

NaCl .............................................................................................. 0.6g Egg yolk ...................................................................................30.0mL

Preparation of Egg Yolk Emulsion (30% Solution): Add NaCl and egg yolk to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Chapman Tellurite Solution: Composition per 100.0mL: K2TeO3 .......................................................................................... 1.0g

Preparation of Chapman Tellurite Solution: Add K2TeO3 to

distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Polymyxin B Solution: Composition per 100.0mL: Polymyxin B ................................................................................. 1.0g

Preparation of Medium: Add peeled, sliced potato to approximately 500.0mL of distilled/deionized water. Gently heat and bring to boiling. Continue boiling for 30 min. Filter through cheesecloth. Cut up liver into small pieces and add to approximately 150.0mL of distilled/deionized water. Gently heat and bring to boiling. Continue boiling for 30 min. Filter through cheesecloth. Add boiled potato solids, boiled liver solids, and remaining components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Make sure each of the tubes receives a few pieces of liver. Autoclave for 15 min at 15 psi pressure– 121°C.

Transgrow Medium Use: For the cultivation and maintenance of Pseudomonas species.

1805

Trace Elements Solution HO-LE Composition per liter:

TPT 18 Medium (DSMZ Medium 1127) Composition per liter: Glucose ........................................................................................ 0.5g Yeast extract ................................................................................. 0.1g Pancreatic digest of casein ............................................................ 0.1g MgSO4·7H2O .............................................................................. 0.05g CaCl2·2H2O................................................................................. 0.02g pH 6.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Adjust pH to 6.0.

Use: For the cultivation of Mucilaginibacter gracilis.

H3BO3 ......................................................................................... 2.85g MnCl2·4H2O ................................................................................. 1.8g Sodium tartrate............................................................................ 1.77g FeSO4·7H2O................................................................................ 1.36g CoCl2·6H2O ................................................................................ 0.04g CuCl2·2H2O............................................................................... 0.027g Na2MoO4·2H2O ........................................................................ 0.025g ZnCl2 ........................................................................................... 0.02g

Preparation of Trace Elements Solution HO-LE: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Use: For the enrichment of other media requiring added trace metals.

Transgrow Medium Composition per liter:

TPY Medium Composition per liter: Pancreatic digest of casein .......................................................... 10.0g Glucose ......................................................................................... 5.0g Pancreatic digest of soybean meal ................................................ 5.0g Yeast extract.................................................................................. 2.5g K2HPO4 ......................................................................................... 2.0g Agar .............................................................................................. 1.5g Cysteine·HCl................................................................................. 0.5g MgCl2·6H2O.................................................................................. 0.5g ZnSO4·7H2O ............................................................................... 0.25g CaCl2 ........................................................................................... 0.15g FeCl3 ...........................................................................................1.0μg Tween™ 80 ................................................................................1.0mL pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until dissolved. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of Bifidobacterium species.

TPYG Medium Composition per liter: Pancreatic digest of casein .......................................................... 10.0g Peptone.......................................................................................... 5.0g Yeast extract.................................................................................. 5.0g Glucose solution ......................................................................50.0mL

Glucose Solution: Composition per 100.0mL: Glucose ....................................................................................... 20.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except glucose solution, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 50.0mL of sterile glucose solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Clostridium felsineum. © 2010 by Taylor and Francis Group, LLC

GC agar base..........................................................................730.0mL Hemoglobin solution .............................................................250.0mL Vitox supplement .....................................................................10.0mL VCN antibiotic solution...........................................................10.0mL pH 7.3 ± 0.2 at 25°C

GC Agar Base: Composition per 730.0mL: Special peptone........................................................................... 15.0g Agar ............................................................................................ 20.0g NaCl.............................................................................................. 5.0g K2HPO4......................................................................................... 4.0g Cornstarch..................................................................................... 1.0g KH2PO4......................................................................................... 1.0g pH 7.2 ± 0.2 at 25°C

Preparation of GC Agar Base: Add components of GC medium base and the hemoglobin to distilled/deionized water and bring volume to 730.0mL. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Hemoglobin Solution: Composition per 250.0mL: Hemoglobin .................................................................................. 5.0g

Preparation of Vitox Supplement: Add components to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

1806

Transgrow Medium

distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

um base, aseptically add 250.0mL of sterile hemoglobin solution, 10.0mL of sterile supplement B, and 10.0mL of VCNT antibiotic solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Preparation of Medium: To 730.0mL of cooled, sterile GC agar

Use: For the cultivation and transport of fastidious microorganisms,

base, aseptically add 250.0mL of sterile hemoglobin solution, 10.0mL of sterile Vitox supplement, and 10.0mL of VCN antibiotic solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

especially Neisseria species.

Use: For the cultivation and transport of fastidious microorganisms,

Agar ............................................................................................ 20.0g Hemoglobin ................................................................................ 10.0g Pancreatic digest of casein............................................................ 7.5g Selected meat peptone .................................................................. 7.5g NaCl.............................................................................................. 5.0g K2HPO4......................................................................................... 4.0g Glucose ......................................................................................... 1.5g Cornstarch..................................................................................... 1.0g KH2PO4......................................................................................... 1.0g Supplement solution ................................................................10.0mL VCNT inhibitor........................................................................10.0mL pH 6.7 ± 0.2 at 25°C

Preparation of VCN Antibiotic Solution: Add components to

especially Neisseria species.

Transgrow Medium Composition per liter: GC medium base....................................................................730.0mL Hemoglobin solution..............................................................250.0mL Supplement B...........................................................................10.0mL VCNT antibiotic solution.........................................................10.0mL pH 7.3 ± 0.2 at 25°C

GC Medium Base: Composition per 730.0mL: Proteose peptone No. 3 ............................................................... 15.0g Agar ............................................................................................ 20.0g NaCl .............................................................................................. 5.0g K2HPO4 ......................................................................................... 4.0g Glucose ......................................................................................... 1.5g Cornstarch ..................................................................................... 1.0g KH2PO4 ......................................................................................... 1.0g pH 7.2 ± 0.2 at 25°C

Preparation of GC Medium Base: Add components to distilled/ deionized water and bring volume to 730.0mL. Mix thoroughly. Gently heat until boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Hemoglobin Solution: Composition per 250.0mL: Hemoglobin ................................................................................ 10.0g

Transgrow Medium with Trimethoprim Composition per liter:

Source: This medium is available as a prepared medium from BD Diagnostic Systems. Supplement Solution: Composition per liter: Glucose ..................................................................................... 100.0g L-Cysteine·HCl............................................................................ 25.9g L-Glutamine ................................................................................ 10.0g L-Cystine ....................................................................................... 1.1g Adenine......................................................................................... 1.0g Nicotinamide adenine dinucleotide ............................................ 0.25g Vitamin B12 ................................................................................... 0.1g Thiamine pyrophosphate .............................................................. 0.1g Guanine·HCl ............................................................................... 0.03g Fe(NO3)3·6H2O........................................................................... 0.02g p-Aminobenzoic acid................................................................ 0.013g Thiamine·HCl ............................................................................ 3.0mg

Source: The supplement solution (IsoVitaleX® enrichment) is available from BD Diagnostic Systems. This enrichment may be replaced by supplement VX from BD Diagnostic Systems.

Preparation of Supplement Solution: Add components to dis-

Supplement B contains yeast concentrate, glutamine, coenzyme, cocarboxylase, hematin, and growth factors.

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Preparation of Supplement B: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

VCNT Inhibitor: Composition per 10.0mL:

Source: Supplement B is available as a premixed powder from BD Diagnostic Systems.

VCNT Antibiotic Solution: Composition per 10.0mL: Colistin methane sulfonate......................................................... 7.5mg Trimethoprim lactate.................................................................. 5.0mg Vancomycin ............................................................................... 3.0mg Nystatin.................................................................................. 12,500U © 2010 by Taylor and Francis Group, LLC

Colistin....................................................................................... 7.5mg Trimethoprim lactate.................................................................. 5.0mg Vancomycin ............................................................................... 3.0mg Nystatin.................................................................................. 12,500U

Preparation of VCNT Inhibitor: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except supplement solution and VCNT inhibitor, to distilled/deionized water and bring vol-

Trebouxia Agar

ume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C under 5–30% CO2. Aseptically add 10.0mL of sterile supplement solution and 10.0mL of sterile VCNT inhibitor. Mix thoroughly. Aseptically distribute under 5–30% CO2 into sterile screw-capped tubes.

Use: For the transportation and recovery of pathogenic Neisseria species.

Transgrow Medium without Trimethoprim Composition per liter: Agar ............................................................................................ 20.0g Hemoglobin ................................................................................ 10.0g Pancreatic digest of casein ............................................................ 7.5g Selected meat peptone .................................................................. 7.5g NaCl .............................................................................................. 5.0g K2HPO4 ......................................................................................... 4.0g Glucose ......................................................................................... 1.5g Cornstarch ..................................................................................... 1.0g KH2PO4 ......................................................................................... 1.0g Supplement solution ................................................................10.0mL VCN inhibitor ..........................................................................10.0mL pH 6.7 ± 0.2 at 25°C

Source: This medium is available as a prepared medium from BD Diagnostic Systems. Supplemement Solution: Composition per liter: Glucose ..................................................................................... 100.0g L-Cysteine·HCl............................................................................ 25.9g L-Glutamine................................................................................. 10.0g L-Cystine ....................................................................................... 1.1g Adenine ......................................................................................... 1.0g Nicotinamide adenine dinucleotide ............................................ 0.25g Vitamin B12 ................................................................................... 0.1g Thiamine pyrophosphate............................................................... 0.1g Guanine·HCl ............................................................................... 0.03g Fe(NO3)3·6H2O ........................................................................... 0.02g p-Aminobenzoic acid................................................................ 0.013g Thiamine·HCl ............................................................................ 3.0mg

Source: The supplement solution IsoVitaleX® enrichment is available from BD Diagnostic Systems. This enrichment may be replaced by supplement VX from BD Diagnostic Systems.

Preparation of Supplement Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

VCN Inhibitor: Composition per 10.0mL:

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tion and 10.0mL of sterile VCN inhibitor. Mix thoroughly. Aseptically distribute under 5–30% CO2 into sterile screw-capped tubes.

Use: For the transportation and recovery of pathogenic Neisseria species.

Transport Medium Composition per liter: Sodium glycerophosphate........................................................... 10.0g Agar .............................................................................................. 3.0g Sodium thioglycolate .................................................................... 1.0g CaCl2·2H2O .................................................................................. 0.1g Methylene Blue.......................................................................... 2.0mg pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into screw-capped tubes or vials. Fill tubes nearly to capacity. Leave only enough space so that when a small swab is introduced the tube does not overflow. Autoclave for 10 min at 15 psi pressure–121°C. Tighten caps on tubes.

Use: For the transportation of swab specimens for the recovery of a wide variety of microorganisms, including Neisseria gonorrhoeae.

Transport Medium Stuart Composition per liter: Sodium glycerophosphate........................................................... 10.0g Agar .............................................................................................. 3.0g Sodium thioglycolate .................................................................... 0.9g CaCl2·2H2O .................................................................................. 0.1g Methylene Blue.......................................................................... 2.0mg pH 7.3 ± 0.2 at 25°C

Use: For the transportation of swab specimens for the recovery of a wide variety of microorganisms, including Neisseria gonorrhoeae.

Trebouxia Agar Composition per liter:

Preparation of VCN Inhibitor: Add components to distilled/de-

Bristol's solution ....................................................................850.0mL Soil extract .............................................................................140.0mL Glucose ....................................................................................... 20.0g Agar ............................................................................................ 15.0g Proteose peptone......................................................................... 10.0g

ionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Bristol's Solution: Composition per 1000.1mL:

Preparation of Medium: Add components, except supplement so-

NaNO3 solution........................................................................... 10.0g KH2PO4 solution ........................................................................... 7.0g K2HPO4 solution ........................................................................... 3.0g MgSO4·7H2O solution .................................................................. 3.0g CaCl2 solution ............................................................................... 1.0g

Colistin....................................................................................... 7.5mg Vancomycin ............................................................................... 3.0mg Nystatin.................................................................................. 12,500U

lution and VCN inhibitor, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C under 5–30% CO2. Aseptically add 10.0mL of sterile supplement solu© 2010 by Taylor and Francis Group, LLC

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Treponema bryantii Medium

NaCl solution ................................................................................ 1.0g FeCl3 solution ............................................................................0.1mL

clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

NaNO3 Solution: Composition per 400.0mL:

Use: For the cultivation of Brachiomonas submarina and Trebouxia magna.

NaNO3......................................................................................... 10.0g

Preparation of NaNO3 Solution: Add NaNO3 to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly.

CaCl2 Solution: Composition per 400.0mL: CaCl2 ............................................................................................. 1.0g

Preparation of CaCl2 Solution: Add CaCl2 to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly.

MgSO4·7H2O Solution: Composition per 400.0mL: MgSO4·7H2O ................................................................................ 3.0g

Preparation of MgSO4·7H2O Solution: Add MgSO4·7H2O to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly. K2HPO4 Solution: Composition per 400.0mL: K2HPO4 ......................................................................................... 3.0g

Treponema bryantii Medium Composition per liter: L-Cysteine·HCl ............................................................................. 1.0g NaCl.............................................................................................. 0.9g (NH4)2SO4 .................................................................................... 0.9g K2HPO4....................................................................................... 0.45g KH2PO4....................................................................................... 0.45g MgSO4·7H2O .............................................................................. 0.18g CaCl2·2H2O ................................................................................ 0.12g Resazurin ................................................................................... 1.0mg Rumen fluid, clarified............................................................300.0mL NaHCO3 solution ...................................................................100.0mL Glucose solution (10% w/v) ....................................................20.0mL pH 7.0 ± 0.2 at 25°C

NaHCO3 Solution: Composition per 100.0mL: NaHCO3 ........................................................................................ 5.0g

Preparation of K2HPO4 Solution: Add K2HPO4 to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly.

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Sparge with 100% CO2.

KH2PO4 Solution: Composition per 400.0mL:

Glucose Solution: Composition per 20.0mL:

KH2PO4 ......................................................................................... 7.0g

Glucose ......................................................................................... 2.0g

Preparation of KH2PO4 Solution: Add KH2PO4 to distilled/de-

Preparation of Glucose Solution: Add glucose to distilled/deion-

NaCl Solution: Composition per 400.0mL:

Preparation of Medium: Prepare and dispense medium under

ionized water and bring volume to 400.0mL. Mix thoroughly.

NaCl .............................................................................................. 1.0g

Preparation of NaCl Solution: Add NaCl to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly.

FeCl3 Solution: Composition per 100.0mL: FeCl3 ............................................................................................. 1.0g

Preparation of FeCl3 Solution: Add FeCl3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Preparation of Bristol's Solution: Add 10.0mL of NaNO3 solu-

tion, 10.0mL of CaCl2 solution, 10.0mL of MgSO4·7H2O solution, 10.0mL of NaNO3 solution, 10.0mL of K2HPO4 solution, 10.0mL of KH2PO4 solution, and 10.0mL of NaCl solution to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Add 0.1mL of FeCl3 solution. Mix thoroughly.

Soil Extract: Composition per 200.0mL: African Violet soil....................................................................... 77.0g Na2CO3 ......................................................................................... 0.2g

Preparation of Soil Extract: Add components to distilled/deionized water and bring volume to 200.0mL. Mix thoroughly. Autoclave for 60 min at 15 psi pressure–121°C. Filter through Whatman #1 filter paper.

ized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize. Sparge with 100% CO2. 100% CO2. Add components, except rumen fluid, NaHCO3 solution, and glucose solution, to distilled/deionized water and bring volume to 580.0mL. Mix thoroughly. Adjust pH to 7.0 with KOH. Sparge with 100% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add 300.0mL of sterile rumen fluid, 100.0mL of sterile NaHCO3 solution, and 20.0mL of sterile glucose solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Treponema bryantii.

Treponema denticola Medium (DSMZ Medium 909) Composition per 1045.0mL: Solution A.....................................................................................1.0L Solution B ................................................................................45.0mL pH 7.1 ± 0.2 at 25°C Solution A: Composition per liter: Trypticase™................................................................................ 10.0g Yeast extract.................................................................................. 2.5g Agar .............................................................................................. 3.0g Glucose ......................................................................................... 2.0g L-Cysteine·HCl ............................................................................. 1.0g Na-thioglycolate ........................................................................... 0.5g L-Asparagine............................................................................... 0.25g Brain heart infusion broth......................................................450.0mL

Treponema Isolation Medium Preparation of Solution A: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Boil for 5 min. Cool to room temperature while sparging with 80% N2 + 20% CO2. Distribute to anaerobe tubes or bottles under 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Brain Heart Infusion Broth: Composition per liter: Pancreatic digest of gelatin ......................................................... 14.5g Brain heart, solids from infusion .................................................. 6.0g Peptic digest of animal tissue........................................................ 6.0g NaCl .............................................................................................. 5.0g Casein............................................................................................ 5.0g Glucose ......................................................................................... 3.0g Na2HPO4 ....................................................................................... 2.5g

Preparation of Brain Heart Infusion Broth: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Solution B: Composition per 65.0mL: NaHCO3 ........................................................................................ 2.0g Thiamine pyrophosphate............................................................ 6.0mg Volatile fatty acid solution .......................................................20.0mL Rabbit serum ............................................................................20.0mL

Volatile Fatty Acid Solution: Composition per 102.0mL: KOH, 0.1N .............................................................................100.0mL Isobutyric acid............................................................................0.5mL D,L-2-methylbutyric acid ...........................................................0.5mL Isovaleric acid ............................................................................0.5mL Valeric acid ................................................................................0.5mL

Preparation of Volatile Fatty Acid Solution: Combine components and mix thoroughly.

Preparation of Solution B: Add components, except rabbit serum, to distilled/deionized water and bring volume to 45.0mL. Mix thoroughly. Filter sterilize. Add 20.0mL rabbit serum.

Preparation of Medium: Aseptically and anaerobically add 0.45mL solution B and 10.0mL solution A to individual tubes. Pressurize the tubes with H2 to 0.5 bar.

Use: For the cultivation of Treponema denticola.

Treponema Isolation Medium Composition per liter: Solution A ..............................................................................450.0mL Spirolate broth........................................................................450.0mL Rabbit serum, inactivated at 56°C for 30 min ........................................100.0mL pH 7.4 ± 0.2 at 25°C

Solution A: Composition per 450.0mL: Agar .............................................................................................. 8.0g Asparagine .................................................................................. 0.25g Sodium thioglycolate .................................................................. 0.25g Pancreatic digest of casein .......................................................... 0.25g Brain heart infusion broth ......................................................450.0mL

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Brain Heart Infusion Broth: Composition per liter: Pancreatic digest of gelatin......................................................... 14.5g Brain heart, solids from infusion .................................................. 6.0g Peptic digest of animal tissue ....................................................... 6.0g NaCl.............................................................................................. 5.0g Casein ........................................................................................... 5.0g Glucose ......................................................................................... 3.0g Na2HPO4 ....................................................................................... 2.5g

Preparation of Brain Heart Infusion Broth: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Spirolate Broth: Composition per liter: Pancreatic digest of casein.......................................................... 15.0g Glucose ......................................................................................... 5.0g Yeast extract.................................................................................. 5.0g NaCl.............................................................................................. 2.5g L-Cysteine·HCl·H2O ..................................................................... 1.0g Sodium thioglycolate .................................................................... 0.5g Palmitic acid ............................................................................... 0.05g Stearic acid.................................................................................. 0.05g Oleic acid .................................................................................... 0.05g Linoleic acid ............................................................................... 0.05g

Preparation of Spirolate Broth: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Preparation of Medium: Combine 450.0mL of sterile solution A, 450.0mL of sterile spirolate broth, and 100.0mL of rabbit serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the isolation and cultivation of oral, genital, and fecal treponemes.

Treponema Isolation Medium Composition per liter: Beef heart, solids from infusion.................................................. 20.0g Ionagar No. 2 ................................................................................ 7.2g K2HPO4......................................................................................... 2.0g Arabinose...................................................................................... 0.8g Glucose ......................................................................................... 0.8g Maltose ......................................................................................... 0.8g Polypeptone™ .............................................................................. 0.8g Pyruvate ........................................................................................ 0.8g Starch, soluble............................................................................... 0.8g Sucrose.......................................................................................... 0.8g Cysteine·HCl............................................................................... 0.68g (NH4)2SO4 .................................................................................... 0.6g Serine ............................................................................................ 0.4g Tryptose ........................................................................................ 0.4g Yeast extract.................................................................................. 0.4g NaCl.............................................................................................. 0.2g Rumen fluid ...........................................................................500.0mL Rabbit serum-cocarboxylase solution....................................100.0mL pH 7.2 ± 0.2 at 25°C

Preparation of Solution A: Combine components. Mix thorough-

Rabbit Serum-Cocarboxylase Solution: Composition per liter:

ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Rabbit serum, heat inactivated...............................................100.0mL Cocarboxylase solution..............................................................1.0mL

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Treponema macrodentium Medium

Preparation of Rabbit Serum-Cocarboxylase Solution: Heat rabbit serum at 56°C for 1 hr. Add 1.0mL of cocarboxylase solution. Mix thoroughly.

Cocarboxylase Solution: Composition per 1.0mL:

rum. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of oral treponemes.

Treponema Medium

Cocarboxylase............................................................................... 0.5g

Composition per liter:

Preparation of Cocarboxylase Solution: Add cocarboxylase to

Spirolate agar .........................................................................900.0mL Rabbit serum, inactivated at 56°C for 30 min ........................................100.0mL

1.0mL of distilled/deionized water. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except rumen fluid and rabbit serum-cocarboxylase solution, to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 500.0mL of sterile rumen fluid and 100.0mL of sterile rabbit serum-cocarboxylase solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation of oral treponemes.

Treponema macrodentium Medium Composition per liter: Glucose ......................................................................................... 1.0g Nicotinamide................................................................................. 0.4g Spermine·4HCl ........................................................................... 0.15g Sodium isobutyrate ..................................................................... 0.02g Carboxylase ............................................................................... 5.0mg PPLO agar..............................................................................900.0mL Bovine serum .........................................................................100.0mL pH 7.0 ± 0.2 at 25°C

PPLO Agar: Composition per 900.0mL: Beef heart, infusion from ............................................................ 50.0g Agar ............................................................................................ 14.0g Peptone........................................................................................ 10.0g NaCl .............................................................................................. 5.0g pH 7.8 ± 0.2 at 25°C

Preparation of PPLO Agar: Add components to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Preparation of Medium: Combine components, except bovine serum. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile bovine serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the isolation and cultivation of Treponema macrodentium.

Treponema Medium Composition per liter: Pancreatic digest of casein .......................................................... 30.0g Ionagar No. 2 ................................................................................ 8.0g Glucose ......................................................................................... 5.0g Yeast extract.................................................................................. 5.0g NaCl .............................................................................................. 2.5g Cysteine·HCl............................................................................... 0.75g Horse serum, inactivated........................................................100.0mL pH 7.4 ± 0.2 at 25°C

Spirolate Agar: Composition per liter: Pancreatic digest of casein.......................................................... 15.0g Agar ............................................................................................ 14.0g Glucose ......................................................................................... 5.0g Yeast extract.................................................................................. 5.0g NaCl.............................................................................................. 2.5g L-Cysteine·HCl·H2O...................................................................... 1.0g Sodium thioglycolate .................................................................... 0.5g Palmitic acid ............................................................................... 0.05g Stearic acid.................................................................................. 0.05g Oleic acid .................................................................................... 0.05g Linoleic acid ............................................................................... 0.05g

Preparation of Spirolate Agar: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Preparation of Medium: To 900.0mL of cooled, sterile spirolate agar, aseptically add 100.0mL of rabbit serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the isolation of oral treponemes.

Treponema Medium Composition per liter: Spirolate agar .........................................................................675.0mL Brain heart infusion broth......................................................225.0mL Rabbit serum, inactivated at 56°C for 30 min ........................................100.0mL pH 7.0–7.2 ± 0.2 at 25°C

Spirolate Agar: Composition per 675.0mL: Pancreatic digest of casein.......................................................... 15.0g Ionagar No. 2 ................................................................................ 8.0g Glucose ......................................................................................... 5.0g Yeast extract.................................................................................. 5.0g NaCl.............................................................................................. 2.5g L-Cysteine·HCl·H2O...................................................................... 1.0g Sodium thioglycolate .................................................................... 0.5g Palmitic acid ............................................................................... 0.05g Stearic acid.................................................................................. 0.05g Oleic acid .................................................................................... 0.05g Linoleic acid ............................................................................... 0.05g

Preparation of Spirolate Agar: Add components to distilled/deionized water and bring volume to 675.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Brain Heart Infusion Broth: Composition per liter: Pancreatic digest of gelatin......................................................... 14.5g Brain heart, solids from infusion .................................................. 6.0g

Treponema Medium

Peptic digest of animal tissue........................................................ 6.0g NaCl .............................................................................................. 5.0g Casein............................................................................................ 5.0g Glucose ......................................................................................... 3.0g Na2HPO4 ....................................................................................... 2.5g

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Preparation of Mucin Solution: Add mucin to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Aseptically combine 440.0mL of solu-

Preparation of Brain Heart Infusion Broth: Add components

tion A, 440.0mL of spirolate broth, 100.0mL of rabbit serum, and 20.0mL of mucin solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Use: For the isolation of intestinal treponemes.

Preparation of Medium: Aseptically combine 675.0mL of cooled, sterile spirolate agar, 225.0mL of cooled, sterile brain heart infusion broth, and 100.0mL of rabbit serum. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation of oral treponemes.

Treponema Medium Composition per liter: Solution A ..............................................................................440.0mL Spirolate broth........................................................................440.0mL Rabbit serum, inactivated at 56°C for 30 min ........................................100.0mL Mucin solution .........................................................................20.0mL pH 7.8 ± 0.2 at 25°C

Solution A: Composition per 440.0mL: Ionagar No. 2 ................................................................................ 8.0g Brain heart infusion broth ......................................................440.0mL

Preparation of Brain Heart Infusion Broth: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Solution A: Add 8.0g of ionagar to 440.0mL of brain heart infusion broth. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Spirolate Broth: Composition per liter: Pancreatic digest of casein .......................................................... 15.0g Glucose ......................................................................................... 5.0g Yeast extract.................................................................................. 5.0g NaCl .............................................................................................. 2.5g L-Cysteine·HCl·H2O...................................................................... 1.0g Sodium thioglycolate .................................................................... 0.5g Palmitic acid ............................................................................... 0.05g Stearic acid.................................................................................. 0.05g Oleic acid .................................................................................... 0.05g Linoleic acid ............................................................................... 0.05g

Preparation of Spirolate Broth: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°. Mucin Solution: Composition per 20.0mL: Mucin ............................................................................................ 0.2g © 2010 by Taylor and Francis Group, LLC

Treponema Medium Composition per liter: Agar ............................................................................................ 13.0g Glucose ......................................................................................... 1.4g Cysteine·HCl............................................................................... 0.64g (NH4)2SO4 .................................................................................... 0.5g Polypeptone™ .............................................................................. 0.5g Starch, soluble............................................................................... 0.5g Yeast extract.................................................................................. 0.5g Resazurin ................................................................................... 1.6mg Salts solution..........................................................................500.0mL Bovine rumen fluid ................................................................280.0mL pH 7.2–7.5 at 25°C

Salts Solution: Composition per liter: NaHCO3 ...................................................................................... 10.0g NaCl.............................................................................................. 2.0g K2HPO4......................................................................................... 1.0g KH2PO4......................................................................................... 1.0g CaCl2 ............................................................................................. 0.2g MgSO4 .......................................................................................... 0.2g CoCl........................................................................................... 3.4mg MnSO4 ....................................................................................... 3.4mg NaMoO4 ..................................................................................... 3.4mg

Preparation of Salts Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, except bovine rumen fluid, to distilled/deionized water and bring volume to 720.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add bovine rumen fluid. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation of intestinal treponemes.

Treponema Medium Composition per liter: Cysteine·HCl·H2O ........................................................................ 1.0g Glucose ......................................................................................... 1.0g Nicotinamide................................................................................. 0.4g Spermidine·4HCl ........................................................................ 0.15g Sodium isobutyrate ..................................................................... 0.02g Thiamine pyrophosphate ........................................................... 5.0mg PPLO broth ............................................................................900.0mL Rabbit serum, inactivated ......................................................100.0mL pH 7.8 ± 0.2 at 25°C

PPLO Broth: Composition per 900.0mL: Beef heart, infusion from solids.................................................. 50.0g Peptone ....................................................................................... 10.0g NaCl.............................................................................................. 5.0g

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Treponema Medium

Preparation of PPLO Broth: Add components to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly.

Preparation of Medium: Combine components, except rabbit serum. Mix thoroughly. Filter sterilize. Aseptically add sterile rabbit serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of oral treponemes. For the cultivation of Treponema denticola, Treponema macrodentium, and Treponema oralis.

Treponema Medium Composition per liter: Spirolate broth........................................................................675.0mL Brain heart infusion broth ......................................................225.0mL Rabbit serum ..........................................................................100.0mL

Preparation of Spirolate Broth: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Preparation of Brain Heart Infusion Broth: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Preparation of Medium: Aseptically combine 675.0mL of cooled, sterile spirolate broth, 225.0mL of cooled, sterile brain heart infusion broth, and 100.0mL of rabbit serum. Mix thoroughly.

Use: For the cultivation of oral treponemes.

Asparagine .................................................................................... 2.5g Sodium thioglycolate .................................................................... 2.5g Pancreatic digest of casein............................................................ 2.5g

Preparation of Heart Infusion Broth, Modified: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Spirolate Broth: Composition per liter: Pancreatic digest of casein.......................................................... 15.0g Glucose ......................................................................................... 5.0g Yeast extract.................................................................................. 5.0g NaCl.............................................................................................. 2.5g L-Cysteine·HCl·H2O...................................................................... 1.0g Sodium thioglycolate .................................................................... 0.5g Palmitic acid ............................................................................... 0.05g Stearic acid.................................................................................. 0.05g Oleic acid .................................................................................... 0.05g Linoleic acid ............................................................................... 0.05g

Preparation of Spirolate Broth: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Preparation of Medium: Aseptically combine 450.0mL of cooled, sterile spirolate broth, 450.0mL of cooled, sterile heart infusion broth, modified, and 100.0mL of rabbit serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of treponemes.

Treponema Medium Composition per 500.0mL: Beef heart, solids from infusion................................................ 250.0g Sucrose........................................................................................ 50.0g Tryptose ........................................................................................ 5.0g NaCl.............................................................................................. 2.5g Yeast extract.................................................................................. 2.5g Agar .............................................................................................. 0.5g Sodium thioglycolate .................................................................. 0.38g MgSO4 ........................................................................................ 0.05g Horse serum, inactivated .......................................................100.0mL pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components, except horse serum, to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly. Adjust pH to 7.4. Gently heat and bring to boiling. Distribute into tubes in 4.0mL volumes. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 25°C. Prior to inoculation, add 1.0mL sterile horse serum to each tube.

Use: For the cultivation and maintenance of Treponema pallidum and

Treponema Medium Composition per liter: Heart infusion broth, modified...............................................450.0mL Spirolate broth........................................................................450.0mL Rabbit serum, inactivated ......................................................100.0mL pH 7.4 ± 0.2 at 25°C

Heart Infusion Broth, Modified: Composition per liter: Beef heart, solids from infusion................................................ 500.0g Tryptose ...................................................................................... 10.0g NaCl .............................................................................................. 5.0g © 2010 by Taylor and Francis Group, LLC

other Treponema species.

Treponema Medium 1 Composition per liter: Thioglycolate agar USP, alternate..........................................900.0mL Normal calf serum .................................................................100.0mL pH 7.1 ± 0.2 at 25°C

Thioglycolate Agar USP, Alternate: Composition per 900.0mL: Pancreatic digest of casein.......................................................... 15.0g Ionagar No. 2 ................................................................................ 7.0g

Treponema saccharophilum Medium

1813

Glucose ......................................................................................... 5.5g Yeast extract.................................................................................. 5.0g NaCl .............................................................................................. 2.5g L-Cystine ....................................................................................... 0.5g Sodium thioglycolate .................................................................... 0.5g

Peptic digest of animal tissue ....................................................... 6.0g NaCl.............................................................................................. 5.0g Casein ........................................................................................... 5.0g Glucose ......................................................................................... 3.0g Na2HPO4 ....................................................................................... 2.5g

Preparation of Thioglycolate Agar USP, Alternate: Add com-

Preparation of Brain Heart Infusion Broth: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

ponents to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Preparation of Medium: Aseptically combine 900.0mL of cooled sterile thioglycolate agar USP, alternate, and 100.0mL of calf serum. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation of treponemes.

Treponema Medium 2 Composition per liter: Pancreatic digest of casein .......................................................... 30.0g Ionagar No. 2 ................................................................................ 7.0g Glucose ......................................................................................... 5.0g Yeast extract.................................................................................. 5.0g NaCl .............................................................................................. 2.5g L-Cysteine·HCl·H2O...................................................................... 2.0g Rabbit serum ..........................................................................100.0mL pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except rabbit serum, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile rabbit serum. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation of treponemes.

Treponema Medium 3 Composition per liter: Spirolate agar .........................................................................675.0mL Brain heart infusion broth ......................................................225.0mL Rabbit serum ..........................................................................100.0mL

Spirolate Agar: Composition per liter: Pancreatic digest of casein .......................................................... 15.0g Ionagar No. 2 ................................................................................ 7.0g Glucose ......................................................................................... 5.0g Yeast extract.................................................................................. 5.0g NaCl .............................................................................................. 2.5g L-Cysteine·HCl·H2O...................................................................... 1.0g Sodium thioglycolate .................................................................... 0.5g Palmitic acid ............................................................................... 0.05g Stearic acid.................................................................................. 0.05g Oleic acid .................................................................................... 0.05g Linoleic acid ............................................................................... 0.05g

Preparation of Medium: Aseptically combine 675.0mL of cooled, sterile spirolate broth, 225.0mL of cooled, sterile brain heart infusion broth, and 100.0mL of rabbit serum. Mix thoroughly.

Use: For the cultivation of treponemes.

Treponema Medium, Prereduced Composition per liter: Agar .............................................................................................. 1.6g Glucose ......................................................................................... 1.4g Cysteine·HCl·H2O ...................................................................... 0.64g (NH4)2SO4 .................................................................................... 0.5g Polypeptone™ .............................................................................. 0.5g Starch, soluble............................................................................... 0.5g Yeast extract.................................................................................. 0.5g Resazurin ................................................................................... 1.6mg Salts solution..........................................................................500.0mL Bovine rumen fluid ................................................................280.0mL pH 7.2–7.5 at 25°C

Preparation of Salts Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, except bovine rumen fluid, to distilled/deionized water and bring volume to 720.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 280.0mL of sterile bovine rumen fluid. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or flasks under 100% N2.

Use: For the cultivation of fecal and intestinal treponemes.

Treponema saccharophilum Medium Composition per liter: Pancreatic digest of casein............................................................ 2.0g Yeast extract.................................................................................. 2.0g L-Cysteine·HCl ............................................................................. 1.0g Resazurin ................................................................................... 1.0mg Salt solution A .......................................................................200.0mL Salt solution B........................................................................200.0mL NaHCO3 solution ...................................................................100.0mL Glucose solution ......................................................................20.0mL

1814

Treponema succinifaciens Medium

iso-Butyric acid..........................................................................0.4mL n-Butyric acid ............................................................................0.4mL DL-2-Methylbutyric acid ............................................................0.2mL iso-Valeric acid ..........................................................................0.2mL n-Valeric acid .............................................................................0.2mL pH 6.7–7.0 at 25°C

Salt Solution A: Composition per liter: MgSO4·7H2O .............................................................................. 0.96g CaCl2·2H2O................................................................................. 0.59g

Preparation of Salt Solution A: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Salt Solution B: Composition per liter: K2HPO4 ...................................................................................... .2.25g KH2PO4 ....................................................................................... 2.25g NaCl .............................................................................................. 4.5g (NH4)2SO4 ..................................................................................... 4.5g

Preparation of Salt Solution B: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

NaHCO3 Solution: Composition per 100.0mL: NaHCO3 ........................................................................................ 5.0g

Cysteine-HCl·H2O ........................................................................ 0.5g KH2PO4......................................................................................... 0.5g Yeast extract.................................................................................. 0.5g Peptone ......................................................................................... 0.5g (NH4)2SO4 .................................................................................... 0.5g CaCl2·2H2O .................................................................................. 0.1g MgSO4·7H2O ................................................................................ 0.1g Resazurin .................................................................................. 0.001g Rumen fluid, clarified............................................................300.0mL

Preparation of Solution A: Add components to 575.0mL of distilled/deionized water. Mix thoroughly. Adjust pH to 6.2–6.3 with 4N HCl. Heat to boiling point. Cool to room temperature under 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Solution B: Composition per 100.0mL: Glucose ....................................................................................... 20.0g

Preparation of Solution B: Add glucose to 100.0mL of distilled/deionized water. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Solution C: Composition per 100.0mL: KH2PO4....................................................................................... 0.45g Na2HPO4·2H2O........................................................................... 0.58g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Sparge with 100% CO2.

Preparation of Solution C: Add components to distilled/deionized

Glucose Solution: Composition per 20.0mL:

Solution D: Composition per 100.0mL:

Glucose ......................................................................................... 2.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize. Sparge with 100% CO2.

Preparation of Medium: Prepare and dispense medium under 100% CO2. Add components, except NaHCO3 solution and glucose solution, to distilled/deionized water and bring volume to 880.0mL. Mix thoroughly. Adjust pH to 7.0 with KOH. Gently heat and bring to boiling. Continue boiling for 5 min. Cool to room temperature while sparging with 100% CO2. Anaerobically distribute 8.8mL into anaerobic culture tubes. Autoclave for 15 min at 15 psi pressure–121°C. Using a syringe, add 1.0mL of sterile NaHCO3 solution and 0.2 mL of sterile glucose solution to each tube. Check that final pH is 6.7–7.0.

Use: For the cultivation and maintenance of Treponema saccharophilum.

Treponema succinifaciens Medium (DSMZ Medium 275) Composition per liter:

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

NaHCO3 ........................................................................................ 5.0g

Preparation of Solution D: Add NaHCO3 to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Filter sterilize.

Preparation of Medium: Distribute solution A under 100% N2 into anaerobic tubes. Autoclave for 20 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically and anaerobically add appropriate amounts of solutions B, C, and D to achieve final concentrations. Use: For the cultivation of Treponema succinifaciens.

Tributyrin Agar Composition per liter: Agar ............................................................................................ 15.0g Tributyrin (glyceryl tributyrate).................................................. 10.0g Peptone ......................................................................................... 5.0g Yeast extract.................................................................................. 3.0g pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a prepared medium from Oxoid

Solution A ..............................................................................875.0mL Solution B ................................................................................50.0mL Solution D ................................................................................50.0mL Solution C ................................................................................25.0mL pH 7.0 ± 0.2 at 25°C

Unipath.

Solution A: Composition per 875.0mL:

Use: For the cultivation and enumeration of lipolytic fungi and bacte-

NaCl .............................................................................................. 1.0g K2HPO4 ......................................................................................... 0.5g © 2010 by Taylor and Francis Group, LLC

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes. ria, especially Staphylococcus species, Flavobacterium species, Clostridium species, and Pseudomonas species from butter. Lipolytic bacteria appear as colonies surrounded by a clear zone.

Trichomonas HiVeg Agar Base with Serum and Selective Supplement

Tributyrin HiVeg Agar Base with Tributyrin Composition per liter: Agar ............................................................................................ 15.0g Plant peptone................................................................................. 5.0g Yeast extract.................................................................................. 3.0g Tributyrin (glyceryl tributyrate)...............................................10.0mL pH 7.5 ± 0.2 at 25°C

Source: This medium, without tributyrin, is available as a premixed powder from HiMedia.

1815

Agar .............................................................................................. 1.0g Horse serum .............................................................................80.0mL pH 6.4 ± 0.2 at 25°C

Source: This medium, without horse serum, is available as a premixed powder from HiMedia.

Horse Serum: Composition per 80.0mL: Horse serum .............................................................................80.0mL

Preparation of Medium: Add components to distilled/deionized

Preparation of Horse Serum: Gently heat sterile horse serum to

water and bring volume to 990.0mL. Add 10.0mL of tributyrin. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°– 50°C. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

56°C for 30 min. Aseptically adjust pH to 6.0 with 0.1N HCl. Use immediately.

Use: For the detection of lipolytic microorganisms.

Trichlorophenol Medium Composition per liter: Pancreatic digest of casein ............................................................ 8.5g NaCl .............................................................................................. 2.5g Papaic digest of soybean meal ...................................................... 1.5g K2HPO4 ....................................................................................... 1.25g Glucose ....................................................................................... 1.25g 2,4,6-Trichlorophenol ................................................................. 1.25g pH 7.3 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Arthrobacter species and other microorganisms that can degrade chlorinated phenols.

Trichococcus Medium Composition per liter: Pancreatic digest of casein .......................................................... 10.0g Na2SO4 .......................................................................................... 4.0g MgCl2·6H2O.................................................................................. 1.1g KCl................................................................................................ 0.7g Glucose solution ......................................................................30.0mL pH 7.3 ± 0.2 at 25°C

Glucose Solution: Composition per 30.0mL: Glucose ......................................................................................... 3.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 30.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except glucose solution, to distilled/deionized water and bring volume to 970.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 30.0mL of sterile glucose solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Trichococcus flocculiformis.

Trichomonas HiVeg Agar Base with Serum Composition per liter: Plant extract No. 2 ...................................................................... 25.0g NaCl .............................................................................................. 6.5g Glucose ......................................................................................... 5.0g © 2010 by Taylor and Francis Group, LLC

Preparation of Medium: Add components, except horse serum, to distilled/deionized water and bring volume to 920.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 80.0mL of freshly prepared sterile horse serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Trichomonas vaginalis.

Trichomonas HiVeg Agar Base with Serum and Selective Supplement Composition per liter: Plant extract No. 2 ...................................................................... 25.0g NaCl.............................................................................................. 6.5g Glucose ......................................................................................... 5.0g Agar .............................................................................................. 1.0g Horse serum .............................................................................80.0mL Selective supplement ...............................................................10.0mL pH 6.4 ± 0.2 at 25°C

Source: This medium, without horse serum or selective supplement, is available as a premixed powder from HiMedia.

Horse Serum: Composition per 80.0mL: Horse serum .............................................................................80.0mL

Preparation of Horse Serum: Gently heat sterile horse serum to 56°C for 30 min. Aseptically adjust pH to 6.0 with 0.1N HCl. Use immediately.

Selective Supplement: Composition per 10.0mL: Streptomycin................................................................................. 0.5g Penicllin ............................................................................ 1,000,000U

Preparation of Selective Supplement: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except horse serum and selective supplement, to distilled/deionized water and bring volume to 910.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 80.0mL of freshly prepared sterile horse serum and 10.0mL sterile selective supplement. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Trichomonas vaginalis.

1816

Trichomonas Medium

Trichomonas Medium Composition per liter:

Horse Serum: Composition per 80.0mL:

Liver digest ................................................................................. 25.0g NaCl .............................................................................................. 6.5g Glucose ......................................................................................... 5.0g Agar .............................................................................................. 1.0g Horse serum .............................................................................80.0mL pH 6.4 ± 0.2 at 25°C

Horse serum .............................................................................80.0mL

Source: This medium is available as a premixed powder from Oxoid

Streptomycin.......................................................................... 500.0mg Penicillin G ....................................................................... 1,000,000U

Unipath.

Horse Serum: Composition per 80.0mL:

Preparation of Horse Serum: Gently heat sterile horse serum to 56°C for 30 min. Aseptically adjust pH to 6.0 with 0.1N HCl. Use immediately.

Antibiotic Inhibitor: Composition per 10.0mL:

Preparation of Antibiotic Inhibitor: Add components to dis-

Horse serum .............................................................................80.0mL

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Horse Serum: Gently heat sterile horse serum to

Preparation of Medium: Add components, except horse serum

56°C for 30 min. Aseptically adjust pH to 6.0 with 0.1N HCl. Use immediately.

and antibiotic inhibitor, to distilled/deionized water and bring volume to 910.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 80.0mL of freshly prepared sterile horse serum and 10.0mL of sterile antibiotic inhibitor. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Trichomonas vaginalis from specimens with a mixed bacterial flora.

Trichomonas Selective Medium Composition per liter:

Trichomonas Medium No. 2 Composition per liter: Glucose ....................................................................................... 22.5g Liver digest ................................................................................. 18.0g Pancreatic digest of casein .......................................................... 17.0g NaCl .............................................................................................. 5.0g Pancreatic digest of soybean meal ................................................ 3.0g K2HPO4 ......................................................................................... 2.5g Chloramphenicol....................................................................... 0.125g Horse serum ...........................................................................250.0mL Calcium pantothenate (0.5% solution).......................................1.0mL pH 6.2 ± 0.2 at 25°C

Source: This medium is available as a prepared medium from Oxoid Unipath.

Preparation of Medium: Add components, except horse serum, to distilled/deionized water and bring volume to 750.0mL. Mix thoroughly. Autoclave for 15 min at 5 psi pressure–108°C. Cool to 45°–50°C. Aseptically add 250.0mL of sterile horse serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the isolation of Trichomonas vaginalis.

Trichomonas Selective Medium Composition per liter: Liver digest ................................................................................. 25.0g NaCl .............................................................................................. 6.5g Glucose ......................................................................................... 5.0g Agar .............................................................................................. 1.0g Horse serum .............................................................................80.0mL Antibiotic inhibitor ..................................................................10.0mL pH 6.4 ± 0.2 at 25°C

Liver digest ................................................................................. 25.0g NaCl.............................................................................................. 6.5g Glucose ......................................................................................... 5.0g Agar .............................................................................................. 1.0g Horse serum .............................................................................80.0mL Antibiotic inhibitor ..................................................................10.0mL pH 6.4 ± 0.2 at 25°C

Horse Serum: Composition per 80.0mL: Horse serum .............................................................................80.0mL

Preparation of Horse Serum: Gently heat sterile horse serum to 56°C for 30 min. Aseptically adjust pH to 6.0 with 0.1N HCl. Use immediately.

Antibiotic Inhibitor: Composition per 10.0mL: Chloramphenicol.................................................................... 100.0mg

Preparation of Antibiotic Inhibitor: Add chloramphenicol to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except horse serum and antibiotic inhibitor, to distilled/deionized water and bring volume to 910.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 80.0mL of freshly prepared sterile horse serum and 10.0mL of sterile antibiotic inhibitor. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Trichomonas vaginalis from specimens with a mixed bacterial flora.

Trichophyton Agar 1 Composition per liter: Glucose ....................................................................................... 40.0g Agar ............................................................................................ 15.0g

Trichophyton Agar 7

Vitamin assay casamino acids....................................................... 2.5g KH2PO4 ......................................................................................... 1.8g MgSO4·7H2O ................................................................................ 0.1g pH 6.8 ± 0.2 at 25°C

1817

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized

agnostic Systems.

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to cool in a slanted position.

Preparation of Medium: Add components to distilled/deionized

Use: For the differentiation of the Trichophyton species.

Source: This medium is available as a premixed powder from BD Di-

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to cool in a slanted position.

Use: For the differentiation of the Trichophyton species.

Trichophyton Agar 2 Composition per liter: Glucose ....................................................................................... 40.0g Agar ............................................................................................ 15.0g Vitamin assay casamino acids....................................................... 2.5g KH2PO4 ......................................................................................... 1.8g MgSO4·7H2O ................................................................................ 0.1g Inositol ..................................................................................... 50.0mg pH 6.8 ± 0.2 at 25°C

Trichophyton Agar 5 Composition per liter: Glucose ....................................................................................... 40.0g Agar ............................................................................................ 15.0g Vitamin assay casamino acids ...................................................... 2.5g KH2PO4......................................................................................... 1.8g MgSO4·7H2O ................................................................................ 0.1g Nicotinic acid............................................................................. 2.0mg pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized

agnostic Systems.

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to cool in a slanted position.

Preparation of Medium: Add components to distilled/deionized

Use: For the differentiation of the Trichophyton species.

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to cool in a slanted position.

Composition per liter:

Source: This medium is available as a premixed powder from BD Di-

Use: For the differentiation of the Trichophyton species.

Trichophyton Agar 3 Composition per liter: Glucose ....................................................................................... 40.0g Agar ............................................................................................ 15.0g Vitamin assay casamino acids....................................................... 2.5g KH2PO4 ......................................................................................... 1.8g MgSO4·7H2O ................................................................................ 0.1g Inositol ........................................................................................ 0.05g Thiamine·HCl ............................................................................ 0.2mg pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di-

Trichophyton Agar 6 Glucose ....................................................................................... 40.0g Agar ............................................................................................ 15.0g KH2PO4......................................................................................... 1.8g Ammonium nitrate........................................................................ 1.5g MgSO4·7H2O ................................................................................ 0.1g pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pressure– 121°C. Allow tubes to cool in a slanted position.

Use: For the differentiation of the Trichophyton species.

agnostic Systems.

Use: For the differentiation of the Trichophyton species.

Trichophyton Agar 4 Composition per liter: Glucose ....................................................................................... 40.0g Agar ............................................................................................ 15.0g Vitamin assay casamino acids....................................................... 2.5g KH2PO4 ......................................................................................... 1.8g MgSO4·7H2O ................................................................................ 0.1g Thiamine·HCl USP .................................................................200.0μg pH 6.8 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

Trichophyton Agar 7 Composition per liter: Glucose ....................................................................................... 40.0g Agar ............................................................................................ 15.0g KH2PO4......................................................................................... 1.8g Ammonium nitrate........................................................................ 1.5g MgSO4·7H2O ................................................................................ 0.1g Histidine·HCl .............................................................................. 0.03g pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

1818

Trichophyton HiVeg Agar 1

Use: For the differentiation of the Trichophyton species.

Trichophyton HiVeg Agar 1 Composition per liter:

MgSO4 .......................................................................................... 0.1g Vitamin-free plant hydrolysate ..................................................... 2.5g Thiamine hydrochloride............................................................. 0.2mg pH 6.8 ± 0.2 at 25°C

Glucose ....................................................................................... 40.0g Agar ............................................................................................ 15.0g Vitamin-free casein enzymic hydrolysate..................................... 2.5g KH2PO4 ......................................................................................... 1.8g MgSO4 .......................................................................................... 0.1g pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-

Use: For the differentiation of the Trichophyton species.

Media.

Use: For the differentiation of the Trichophyton species.

Trichophyton HiVeg Agar 2 Composition per liter: Glucose ....................................................................................... 40.0g Agar ............................................................................................ 15.0g Vitamin-free plant hydrolysate ..................................................... 2.5g KH2PO4 ......................................................................................... 1.8g MgSO4 .......................................................................................... 0.1g Inositol ....................................................................................... 5.0mg pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Use: For the differentiation of the Trichophyton species.

Trichophyton HiVeg Agar 3 Composition per liter: Glucose ....................................................................................... 40.0g Agar ............................................................................................ 15.0g Vitamin-free plant hydrolysate ..................................................... 2.5g KH2PO4 ......................................................................................... 1.8g MgSO4 .......................................................................................... 0.1g Inositol ....................................................................................... 5.0mg Thiamine .................................................................................... 5.0mg pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Use: For the differentiation of the Trichophyton species.

Trichophyton HiVeg Agar 4

Media.

Trichophyton HiVeg Agar 5 Composition per liter: Glucose ....................................................................................... 40.0g Agar ............................................................................................ 15.0g Vitamin-free plant hydrolysate ..................................................... 2.5g KH2PO4......................................................................................... 1.8g MgSO4 .......................................................................................... 0.1g Nicotinic acid.............................................................................. 0.02g pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Use: For the differentiation of the Trichophyton species.

Trichosel™ Broth, Modified Composition per liter: Pancreatic digest of casein.......................................................... 12.0g Yeast extract.................................................................................. 5.0g Liver extract.................................................................................. 2.0g Maltose ......................................................................................... 2.0g L-Cysteine·HCl.............................................................................. 1.0g Agar .............................................................................................. 1.0g Chloramphenicol........................................................................... 0.1g Methylene Blue.......................................................................... 3.0mg Horse serum .............................................................................50.0mL pH 6.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium: Add components, except horse serum, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 13 psi pressure–118°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile horse serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the isolation and cultivation of Trichomonas species.

Trimethylamine N-Oxide Medium (TMAO Medium)

Composition per liter:

Beef extract................................................................................. 10.0g Peptone ....................................................................................... 10.0g NaCl.............................................................................................. 5.0g

Triple Sugar Iron Agar, HiVeg

Agar .............................................................................................. 2.0g Trimethylamine N-oxide ............................................................... 1.0g Yeast extract.................................................................................. 1.0g pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into screw-capped tubes in 4.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to cool in an upright position.

Use: For the cultivation and differentiation of Campylobacter species from foods. Campylobacter jejuni and Campylobacter coli will not grow.

Triple Sugar Iron Agar (TSI Agar) Composition per liter: Peptone........................................................................................ 20.0g Agar ............................................................................................ 12.0g Lactose ........................................................................................ 10.0g Sucrose........................................................................................ 10.0g NaCl .............................................................................................. 5.0g Beef extract ................................................................................... 3.0g Yeast extract.................................................................................. 3.0g Glucose ......................................................................................... 1.0g Ferric citrate .................................................................................. 0.3g Na2S2O3 ........................................................................................ 0.3g Phenol Red ................................................................................ 0.025g pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems and Oxoid Unipath.

Use: For the differentiation of members of the Enterobacteriaceae based on their fermentation of lactose, sucrose, and glucose and the production of H2S.

Triple Sugar Iron Agar (TSI Agar) Composition per liter: Agar ............................................................................................ 13.0g Pancreatic digest of casein .......................................................... 10.0g Peptic digest of animal tissue...................................................... 10.0g Lactose ........................................................................................ 10.0g Sucrose........................................................................................ 10.0g NaCl .............................................................................................. 5.0g Glucose ......................................................................................... 1.0g Fe(NH4)2(SO4)2·6H2O .................................................................. 0.2g Na2S2O3 ........................................................................................ 0.2g Phenol Red ................................................................................ 0.025g pH 7.3 ± 0.2 at 25°C

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Use: For the differentiation of members of the Enterobacteriaceae based on their fermentation of lactose, sucrose, and glucose and the production of H2S.

Triple Sugar Iron Agar (TSI Agar) (BAM M149 Medium 2) Composition per liter: Peptone ....................................................................................... 15.0g Agar ............................................................................................ 12.0g Lactose........................................................................................ 10.0g Sucrose........................................................................................ 10.0g Proteose peptone........................................................................... 5.0g NaCl.............................................................................................. 5.0g Beef extract................................................................................... 3.0g Yeast extract.................................................................................. 3.0g Glucose ......................................................................................... 1.0g Na2S2O3 ........................................................................................ 0.3g FeSO4 ............................................................................................ 0.2g Phenol Red................................................................................ 0.024g pH 7.4 ± 0.2 at 25°C

Use: For the differentiation of members of the Enterobacteriaceae based on their fermentation of lactose, sucrose, and glucose and the production of H2S.

Triple Sugar Iron Agar, HiVeg Composition per liter: Agar ............................................................................................ 12.0g Plant hydrolysate ........................................................................ 10.0g Plant peptone .............................................................................. 10.0g Lactose........................................................................................ 10.0g Sucrose........................................................................................ 10.0g NaCl.............................................................................................. 5.0g Plant extract .................................................................................. 3.0g Yeast extract.................................................................................. 3.0g Glucose ......................................................................................... 1.0g Na2S2O3 ........................................................................................ 0.3g FeSO4 ............................................................................................ 0.2g Phenol Red................................................................................ 0.024g pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

1820

Tris YP Agar

Use: For the differentiation of members of the Enterobacteriaceae based on their fermentation of lactose, sucrose, and glucose and the production of H2S.

Tris YP Agar (Tris Yeast Extract Peptone Agar) Composition per liter: Agar ............................................................................................ 19.0g Yeast extract.................................................................................. 3.0g Glucose ......................................................................................... 1.0g Peptone.......................................................................................... 0.6g Tris buffer (0.05M, pH 7.5)...........................................................1.0L pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. For top layer agar, add 6.0g of agar instead of 19.0g. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes.

Use: For the cultivation and maintenance of Bdellovibrio species.

Tris YP Broth (Tris Yeast Extract Peptone Broth) Composition per liter: Yeast extract.................................................................................. 3.0g Glucose ......................................................................................... 1.0g Peptone.......................................................................................... 0.6g Tris buffer (0.05M, pH 7.5)...........................................................1.0L pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Bdellovibrio species.

Trypaflavin Nalidixic Acid Serum Agar (TNSA Agar) Composition per liter: Ionagar No. 2 .............................................................................. 12.0g Peptone........................................................................................ 10.0g Beef extract ................................................................................... 3.0g H2O ........................................................................................926.5mL Bovine serum, heat inactivated ................................................50.0mL Nalidixic acid solution .............................................................20.0mL Trypaflavin solution...................................................................3.5mL pH 7.2–7.4 at 25°C

Preparation of Medium: Add components—except bovine serum, nalidixic acid solution, and trypaflavin solution—to distilled/deionized water and bring volume to 926.5mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile bovine serum, 20.0mL of sterile nalidixic acid solution, and 3.5mL of trypaflavin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the isolation and cultivation of Listeria species from preenriched specimens.

Trypanosome Medium Composition per 1300.0mL: Solid phase....................................................................................1.0L Liquid phase (Locke’s solution) ............................................300.0mL pH 7.2–7.4 at 25°C

Solid Phase: Composition per liter: Agar ............................................................................................ 15.0g NaCl.............................................................................................. 8.0g Peptone ......................................................................................... 5.0g Beef extract................................................................................... 3.0g Rabbit blood, defibrinated .....................................................300.0mL

Preparation of Solid Phase: Add components, except rabbit blood, to distilled/deionized water and bring volume to 700.0mL. Mix thoroughly. Adjust pH to 7.2–7.4. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 300.0mL of sterile defibrinated rabbit blood. Mix thoroughly. Distribute 10.0mL aliquots into sterile screw-capped tubes. Allow to solidify in a slanted position. Liquid Phase (Locke’s Solution): Composition per liter: NaCl.............................................................................................. 8.0g Glucose ......................................................................................... 2.5g KH2PO4......................................................................................... 0.3g CaCl2 ............................................................................................. 0.2g KCl................................................................................................ 0.2g

Preparation of Liquid Phase (Locke’s Solution): Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Aseptically overlay agar slants (solid phase) with 3.0mL per tube of sterile liquid phase (Locke’s solution).

Use: For the cultivation of Leishmania donovani, Leishmania braziliensis, Trypanosoma gambiense, and Trypanosoma rhodesiense.

Nalidixic Acid Solution: Composition per 10.0mL:

Tryptic Digest Broth

Nalidixic acid .............................................................................. 0.02g

Composition per liter:

Preparation of Nalidixic Acid Solution: Add nalidixic acid to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Tryptic digest of beef heart ......................................................... 10.0g NaCl.............................................................................................. 5.0g Glucose ......................................................................................... 1.0g pH 7.6 ± 0.2 at 25°C

Trypaflavin Solution: Composition per 10.0mL:

Source: This medium is available as a premixed powder from BD Di-

Trypaflavin.................................................................................... 0.1g

agnostic Systems.

Preparation of Trypaflavin Solution: Add trypaflavin to dis-

Preparation of Medium: Add components to distilled/deionized

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Tryptic Soy Agar with 0.6% Yeast Extract Use: For use as a base medium to which enrichments are added. For the cultivation of fastidious microorganisms.

1821

to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes in 20.0mL volumes.

Use: For the cultivation of Escherichia coli from foods.

Tryptic Nitrate Medium Composition per liter: Tryptose ...................................................................................... 20.0g Na2HPO4 ....................................................................................... 2.0g Agar .............................................................................................. 1.0g Glucose ......................................................................................... 1.0g KNO3 ............................................................................................ 1.0g pH 7.6 ± 0.2 at 25°C

Tryptic Soy Agar with Magnesium Sulfate and Sodium Chloride Composition per liter:

agnostic Systems.

Pancreatic digest of casein.......................................................... 50.0g NaCl............................................................................................ 30.0g Agar ............................................................................................ 15.0g Pancreatic digest of soybean meal................................................ 5.0g MgSO4·7H2O ................................................................................ 1.5g pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

Source: This medium is available as a premixed powder from BD Di-

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and differentiation of Pseudomonas and related genera. For the differentiation of bacteria based on their reduction of nitrate to nitrite. After incubation of the bacterium in tryptic nitrate medium for 18–24 hr, sulfanillic acid and α-naphthol reagents are added. Nitrate reduction is indicated by the development of a red to violet color.

Tryptic Soy Agar See: Trypticase™ Soy Agar Tryptic Soy Agar Blood Agar Base See: Trypticase™ Soy Agar with Sheep Blood

Tryptic Soy Agar with Magnesium Ions Composition per liter: Pancreatic digest of casein .......................................................... 17.0g Agar ............................................................................................ 15.0g NaCl .............................................................................................. 5.0g Pancreatic digest of soybean meal ................................................ 3.0g Glucose ......................................................................................... 2.5g K2HPO4 ......................................................................................... 2.5g MgCl2 .......................................................................................... 0.95g pH 7.3 ± 0.2 at 25°C

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes in 20.0mL volumes.

Use: For the cultivation of Vibrio species from foods.

Tryptic Soy Agar with Sodium Chloride (ATCC Medium 2276) Composition per liter: Pancreatic digest of casein.......................................................... 50.0g NaCl............................................................................................ 20.0g Agar ............................................................................................ 15.0g Pancreatic digest of soybean meal................................................ 5.0g MgSO4·7H2O ................................................................................ 1.5g pH 7.3 ± 0.2 at 25°C

Use: For the cultivation of Vibrio species.

Tryptic Soy Agar with 0.6% Yeast Extract Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein.......................................................... 15.0g Yeast extract.................................................................................. 6.0g Pancreatic digest of soybean meal................................................ 5.0g NaCl.............................................................................................. 5.0g pH 7.0–7.5 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Use: For the cultivation of Escherichia coli for bacteriophage produc-

Preparation of Medium: Add components to distilled/deionized

tion.

Tryptic Soy Agar with Magnesium Sulfate Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein .......................................................... 15.0g NaCl .............................................................................................. 5.0g Pancreatic digest of soybean meal ................................................ 5.0g MgSO4·7H2O ................................................................................ 1.5g pH 7.3 ± 0.2 at 25°C

Use: For the isolation and cultivation of Listeria monocytogenes from foods. Tryptic Soy Blood Agar See: Trypticase™ Soy Agar with Sheep Blood Tryptic Soy Blood Agar with VAN 4 See: Trypticase™ Soy Agar with Sheep Blood and Vancomycin

1822

Tryptic Soy Broth

Tryptic Soy Broth with Magnesium Ions (ATCC Medium 1588)

Composition per liter: Pancreatic digest of casein .......................................................... 18.0g Papaic digest of soybean meal ...................................................... 6.0g NaCl .............................................................................................. 6.0g pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Use: For the isolation and cultivation of a wide variety of microorganisms.

Tryptic Soy Broth with 0.001M Calcium Chloride (ATCC Medium 1380) Composition per liter: Pancreatic digest of casein .......................................................... 18.0g Papaic digest of soybean meal ...................................................... 6.0g NaCl .............................................................................................. 6.0g CaCl2 solution ..........................................................................10.0mL pH 7.3 ± 0.2 at 25°C

Source: This medium, without CaCl2, is available as a premixed powder from BD Diagnostic Systems.

Calcium Chloride Solution: Composition per 10.0mL:

Composition per liter: Pancreatic digest of casein.......................................................... 17.0g NaCl.............................................................................................. 5.0g Pancreatic digest of soybean meal................................................ 3.0g Glucose ......................................................................................... 2.5g K2HPO4......................................................................................... 2.5g MgCl2.......................................................................................... 0.95g pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into flasks or tubes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Escherichia coli for bacteriophage production.

Tryptic Soy Broth with 1M Potassium Chloride (ATCC Medium 2074) Composition per liter: KCl.............................................................................................. 74.5g Pancreatic digest of casein.......................................................... 17.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

CaCl2 ......................................................................................... 0.111g

Use: For the cultivation and maintenance of Gracilibacillus dipso-

Preparation of Calcium Chloride Solution: Add CaCl2 to dis-

sauri.

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except CaCl2 solution,

to distilled/deionized water and bring volume to 990mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0mL CaCl2 solution. Mix thoroughly. Distribute into tubes or flasks.

Use: For the isolation and cultivation of Bronchothrix thermospacta.

Tryptic Soy Broth with 0.1% Potassium Nitrate (ATCC Medium 1183) Composition per liter: Pancreatic digest of casein .......................................................... 15.0g Agar ............................................................................................ 15.0g Papaic digest of soybean meal ...................................................... 5.0g NaCl .............................................................................................. 5.0g KNO3 ............................................................................................ 1.0g pH 7.3 ± 0.2 at 25°C

Source: This medium, without nitrate, is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Do not overheat. Pour into sterile Petri dishes or leave in tubes.

Tryptic Soy Fast Green Agar (TSFA) Composition per liter: Pancreatic digest of casein.......................................................... 17.0g Agar ............................................................................................ 15.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Fast Green FCF........................................................................... 0.25g pH 7.3 ± 0.2 at 25°C

Use: For the isolation and cultivation of Salmonella species from foods.

Trypticase™ Agar Base Composition per liter: Pancreatic digest of casein.......................................................... 20.0g Agar .............................................................................................. 3.5g Phenol Red.................................................................................. 0.02g pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems and Oxoid Unipath.

Trypticase™ Peptone Glucose Yeast Extract Broth, Buffered Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the differentiation of microorganisms based on their motility.

Trypticase™ Agar Base with Carbohydrate Composition per liter: Pancreatic digest of casein .......................................................... 20.0g Carbohydrate................................................................................. 5.0g Agar .............................................................................................. 3.5g Phenol Red .................................................................................. 0.02g pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 13 psi pressure–118°C. Do not overheat. Pour into sterile Petri dishes or leave in tubes.

Use: For differentiation of microorganisms based on their motility and fermentation reactions. Fermentation of carbohydrate turns the medium yellow.

Trypticase™ Azolectin Tween™ Broth Base See: TAT Broth Base

Trypticase™ Broth, Supplemented

1823

Beef extract................................................................................... 3.0g Glucose ......................................................................................... 1.0g pH 7.0 ± 0.2 at 25°C

Use: For the enumeration of bacteria in water, milk, and other specimens.

Trypticase™ Novobiocin Broth (TN Broth) Composition per liter: Pancreatic digest of casein.......................................................... 17.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Bile salts No. 3.............................................................................. 1.5g K2HPO4......................................................................................... 1.5g Novobiocin solution.................................................................10.0mL pH 7.3 ± 0.2 at 25°C

Novobiocin Solution: Composition per 10.0mL:

Composition per liter:

Novobiocin ................................................................................. 0.02g

Pancreatic digest of casein .......................................................... 20.0g MgSO4·7H2O ............................................................................ 0.015g FeCl3 .......................................................................................... 7.0mg pH 7.2 ± 0.2 at 25°C

Preparation of Novobiocin Solution: Add novobiocin to dis-

Preparation of Medium: Add components to distilled/deionized

tion, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile novobiocin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Bacillus stearothermophilus.

Trypticase™ Glucose Agar Composition per liter: Pancreatic digest of casein .......................................................... 17.0g Agar ............................................................................................ 15.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g Glucose ......................................................................................... 7.5g Yeast extract.................................................................................. 3.0g KH2PO4 ........................................................................................ 2.5g

Use: For the cultivation and maintenance of Lactococcus raffinolactis, Streptococcus equi, Streptococcus pneumoniae, and Streptococcus uberis.

Trypticase™ Glucose Extract Agar

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except novobiocin solu-

Use: For the cultivation of verotoxin-producing Escherichia coli.

Trypticase™ Peptone Glucose Yeast Extract Broth (TPGY Broth) Composition per liter: Pancreatic digest of casein.......................................................... 50.0g Yeast extract................................................................................ 20.0g Peptone ......................................................................................... 5.0g Glucose ......................................................................................... 4.0g Sodium thioglycolate .................................................................... 1.0g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes in 15.0mL volumes. Autoclave for 10 min at 15 psi pressure–121°C.

Use: For the cultivation of Clostridium botulinum.

Trypticase™ Peptone Glucose Yeast Extract Broth, Buffered

Composition per liter:

Agar ............................................................................................ 15.0g Pancreatic digest of casein ............................................................ 5.0g

Pancreatic digest of casein.......................................................... 50.0g Yeast extract................................................................................ 20.0g

1824

Trypticase™ Peptone Glucose Yeast Extract Broth with Trypsin

Na2HPO4 ....................................................................................... 5.0g Peptone.......................................................................................... 5.0g Glucose ......................................................................................... 4.0g Sodium thioglycolate .................................................................... 1.0g pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until dissolved. Adjust pH to 7.3. Distribute into tubes in 15.0mL volumes. Autoclave for 8 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of Clostridium perfringens from foods.

Trypticase™ Peptone Glucose Yeast Extract Broth with Trypsin (TPGYT Broth) Composition per 1067.0mL: Pancreatic digest of casein .......................................................... 50.0g Yeast extract................................................................................ 20.0g Peptone.......................................................................................... 5.0g Glucose ......................................................................................... 4.0g Sodium thioglycolate .................................................................... 1.0g Trypsin solution .......................................................................67.0mL pH 7.0 ± 0.2 at 25°C

Trypsin Solution: Composition per 100.0mL: Trypsin .......................................................................................... 1.5g

Preparation of Trypsin Solution: Add trypsin to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except trypsin solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 15.0mL volumes. Autoclave for 10 min at 15 psi pressure–121°C. Immediately prior to use, aseptically add 1.0mL of sterile trypsin solution to each tube. Mix thoroughly.

Use: For the cultivation of Clostridium botulinum.

Trypticase™ Phytone™ Glucose Medium Composition per liter: Glucose ....................................................................................... 15.0g Pancreatic digest of casein .......................................................... 10.0g Agar .............................................................................................. 8.0g Papaic digest of soybean meal ...................................................... 5.0g Yeast extract.................................................................................. 2.5g K2HPO4 ......................................................................................... 2.0g L-Cysteine·HCl·H2O...................................................................... 0.5g MgCl2 ............................................................................................ 0.5g ZnSO4·7H2O ............................................................................... 0.25g FeCl3 .......................................................................................... 1.0mg

Preparation of Medium: Add ZnSO4 to approximately 100.0mL of distilled/deionized water and dissolve. Add remaining components and bring volume to 1.0L with distilled/deionized water. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Bifidobacterium species. © 2010 by Taylor and Francis Group, LLC

Trypticase™ Phytone™ Glucose Medium with Tween™ 80 Composition per liter: Glucose ....................................................................................... 15.0g Pancreatic digest of casein.......................................................... 10.0g Agar .............................................................................................. 8.0g Papaic digest of soybean meal...................................................... 5.0g Yeast extract.................................................................................. 2.5g K2HPO4......................................................................................... 2.0g L-Cysteine·HCl·H2O...................................................................... 0.5g MgCl2............................................................................................ 0.5g ZnSO4·7H2O ............................................................................... 0.25g FeCl3 .......................................................................................... 1.0mg Tween™ 80................................................................................2.0mL

Trypticase™ Phytone Medium (DSMZ Medium 75) Composition per liter: Trypticase™ peptone .................................................................. 17.0g Phytone™ peptone........................................................................ 3.0g NaCl.............................................................................................. 5.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Distilled water......................................................................1000.0mL pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation and maintenance of Acinetobacter spp.

Trypticase™ Phytone™ Medium Composition per liter: Pancreatic digest of casein.......................................................... 17.0g NaCl.............................................................................................. 5.0g Phytone™ peptone........................................................................ 3.0g Glucose ......................................................................................... 2.5g K2HPO4......................................................................................... 2.5g pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Acinetobacter species.

Trypticase™ Serum Seawater Agar (ATCC Medium 1359) Composition per liter: Agar ............................................................................................ 12.0g Pancreatic digest of casein............................................................ 1.0g Seawater.................................................................................990.0mL Horse serum .............................................................................10.0mL pH 7.3 ± 0.2 at 25°C

Trypticase™ Soy Agar with Glycerol Preparation of Medium: Add components, except horse serum, to 990.0mL seawater. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile horse serum. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

psi pressure–121°C. Do not overheat. Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation and cultivation of a wide variety of fastidious as well as nonfastidious microorganisms.

Use: For the cultivation and maintenance of fastidious marine bacterial

Trypticase™ Soy Agar with Cefazidime (ATCC Medium 1997)

species.

Trypticase™ Serum Seawater Agar Composition per liter: Agar ............................................................................................ 12.0g Pancreatic digest of casein ............................................................ 1.0g Seawater.................................................................................990.0mL Horse serum .............................................................................10.0mL

Preparation of Medium: Add agar and pancreatic digest of casein to 990.0mL of seawater. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile horse serum. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Diplophyrs marina, Haliphthoros milfordensis, and Ostracoblabe implexa.

Trypticase™ Soy Agar (ATCC Medium 18) Composition per liter: Pancreatic digest of casein .......................................................... 17.0g Agar ............................................................................................ 15.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 2.5g pH 7.3 ± 0.2 at 25°C

Use: For the cultivation and maintenance of a wide variety of heterotrophic microorganisms. For the cultivation of a wide variety of fastidious and nonfastidious microorganisms from clinical and nonclinical specimens. Also used for the rapid estimation of the bacteriological quality of water.

Trypticase™ Soy Agar (Tryptic Soy Agar) (Soybean Casein Digest Agar) (ATCC Medium 77) Composition per liter:

1825

Composition per liter: Pancreatic digest of casein.......................................................... 15.0g Agar ............................................................................................ 15.0g Papaic digest of soybean meal...................................................... 5.0g NaCl.............................................................................................. 5.0g Ceftazidime solution................................................................10.0mL pH 7.3 ± 0.2 at 25°C

Ceftazidime Solution: Composition per 10.0mL: Ceftazidime................................................................................ 4.0mg

Preparation of Ceftazidime Solution: Add ceftazidime to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except ceftazidime solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile ceftazidime solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Klebsiella oxytoca.

Trypticase™ Soy Agar with 1% Glucose Composition per liter: Pancreatic digest of casein.......................................................... 15.0g Agar ............................................................................................ 15.0g Glucose ....................................................................................... 10.0g Papaic digest of soybean meal...................................................... 5.0g NaCl.............................................................................................. 5.0g pH 7.3 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Saccharomonospora viridis.

Trypticase™ Soy Agar with Glycerol Composition per liter:

Pancreatic digest of casein .......................................................... 15.0g Agar ............................................................................................ 15.0g Papaic digest of soybean meal ...................................................... 5.0g NaCl .............................................................................................. 5.0g pH 7.3 ± 0.2 at 25°C

Pancreatic digest of casein.......................................................... 15.0g Agar ............................................................................................ 15.0g Papaic digest of soybean meal...................................................... 5.0g NaCl.............................................................................................. 5.0g Glycerol ...................................................................................50.0mL pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di-

Preparation of Medium: Add components to distilled/deionized

agnostic Systems.

water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Do not overheat. Pour into sterile Petri dishes or leave in tubes. For blood plates, 50.0–100.0mL of sterile

1826

Trypticase™ Soy Agar with Human Blood

defibrinated sheep blood may be added to sterile medium that has been melted and cooled to 45°–50°C.

Use: For the cultivation and maintenance of Acinetobacter calcoaceticus.

Use: For the cultivation of Vibrio species from foods.

Trypticase™ Soy Agar with Human Blood Composition per liter: Pancreatic digest of casein .......................................................... 15.0g Agar ............................................................................................ 15.0g Papaic digest of soybean meal ...................................................... 5.0g NaCl .............................................................................................. 5.0g Human blood, defibrinated ......................................................50.0mL pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except human blood, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile human blood. Mix thoroughly. Pour into sterile Petri dishes in 17.0mL volumes or distribute into sterile tubes.

Use: For the cultivation of a wide variety of fastidious microorganisms. For the observation of hemolytic reactions of a variety of bacteria. May be used to perform the CAMP test for the presumptive identification of group B streptococci (Streptococcus agalactiae).

Trypticase™ Soy Agar with Lecithin and Polysorbate 80 (Microbial Content Test Agar) Composition per liter: Pancreatic digest of casein .......................................................... 15.0g Agar ............................................................................................ 15.0g Papaic digest of soybean meal ...................................................... 5.0g NaCl .............................................................................................. 5.0g Polysorbate 80 (Tween™ 80) ....................................................... 5.0g Lecithin ......................................................................................... 0.7g pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 13 psi pressure–118°C. Cool to 45°–50°C. Pour into sterile Petri dishes in 17.0mL volumes or leave in tubes.

Trypticase™ Soy Agar, Modified Composition per liter: Pancreatic digest of casein.......................................................... 17.0g Agar ............................................................................................ 15.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 4.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g

Use: For the cultivation and maintenance of the Simonsiella species.

Trypticase™ Soy Agar, Modified (ATCC Medium 1386) Composition per liter: Agar ............................................................................................ 18.0g Pancreatic digest of casein.......................................................... 17.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Yeast extract.................................................................................. 0.4g NH4OH, concentrated ............................................................0.035mL pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components, except NH4OH, to dis-

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 45°–50°C. Aseptically add NH4OH. Mix thoroughly. Adjust pH to 7.5 if necessary. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of ATCC strain 31205.

Trypticase™ Soy Agar, Modified (ATCC Medium 1481)

Use: For the detection and enumeration of microorganisms in replicate

Composition per liter:

plating techniques. Also used for the detection and enumeration of microorganisms present on surfaces of sanitary importance.

Composition per liter:

Pancreatic digest of casein.......................................................... 17.0g Agar ............................................................................................ 15.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g L-Glutamine .............................................................................10.0mL pH 6.5 ± 0.2 at 25°C

NaCl ............................................................................................ 20.0g Trypticase peptone ...................................................................... 15.0g Agar ............................................................................................ 15.0g Phytone™ peptone........................................................................ 5.0g MgSO4·7H2O ................................................................................ 1.5g pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except glutamine, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile glutamine. Mix thoroughly. Adjust pH to 6.5. Pour into sterile Petri dishes or distribute into sterile tubes.

Trypticase™ Soy Agar-Magnesium SulfateSodium Chloride Agar (TSAMS) (BAM M152a)

Trypticase™ Soy Agar with Sheep Blood and Gentamicin Use: Used as a base that is supplemented. For the cultivation of fastidious microorganisms. When supplemented with sheep blood, this medium is useful for the observation of hemolytic reactions of a variety of bacteria.

Trypticase™ Soy Agar, Modified (TSA II™) Composition per liter: Pancreatic digest of casein .......................................................... 14.5g Agar ............................................................................................ 14.0g Papaic digest of soybean meal ...................................................... 5.0g NaCl .............................................................................................. 5.0g Growth factors (BBL) ................................................................... 1.5g pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Do not overheat. Pour into sterile Petri dishes or leave in tubes. For blood plates, 50.0–100.0mL of sterile defibrinated sheep blood may be added to sterile medium that has been melted and cooled to 45°–50°C.

Use: Used as a base that is supplemented. For the cultivation of fastid-

1827

Sheep blood, defibrinated ........................................................50.0mL pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except sheep blood, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile sheep blood. Mix thoroughly. Pour into sterile Petri dishes in 17.0mL volumes or distribute into sterile tubes. Use: For the cultivation of a wide variety of fastidious microorganisms. For the observation of hemolytic reactions of a variety of bacteria. May be used to perform the CAMP test for the presumptive identification of group B streptococci (Streptococcus agalactiae).

Trypticase™ Soy Agar with Sheep Blood, Formate, and Fumarate Composition per liter: Pancreatic digest of casein.......................................................... 14.5g Agar ............................................................................................ 14.0g Papaic digest of soybean meal...................................................... 5.0g NaCl.............................................................................................. 5.0g Sucrose.......................................................................................... 2.0g Growth factors .............................................................................. 1.5g Sheep blood, defibrinated ........................................................50.0mL Formate-fumarate solution.......................................................13.0mL pH 7.3 ± 0.2 at 25°C

ious microorganisms. When supplemented with sheep blood, this medium is useful for the observation of hemolytic reactions of a variety of bacteria. It may be used to perform the CAMP test for the presumptive identification of group B streptococci (Streptococcus agalactiae).

Source: Growth Factors are available as a premixed powder from BD

Trypticase™ Soy Agar, Modified with Horse Serum

Sodium formate ............................................................................ 6.0g Fumaric acid ................................................................................. 6.0g

Composition per liter: Pancreatic digest of casein .......................................................... 17.0g Agar ............................................................................................ 15.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 4.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g Horse serum ...........................................................................100.0mL pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except horse serum, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile horse serum. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation and maintenance of Simonsiella species, Alysiella species, and Moraxella species.

Trypticase™ Soy Agar with Sheep Blood (Tryptic Soy Blood Agar) (TSA Blood Agar) Composition per liter: Pancreatic digest of casein .......................................................... 15.0g Agar ............................................................................................ 15.0g Papaic digest of soybean meal ...................................................... 5.0g NaCl .............................................................................................. 5.0g © 2010 by Taylor and Francis Group, LLC

Diagnostic Systems.

Formate-Fumarate Solution: Composition per 100.0mL:

Preparation of Formate-Fumarate Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 7.0. Filter sterilize. Preparation of Medium: Add components, except sheep blood, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 45°–50°C. Aseptically add sterile sheep blood. Mix thoroughly. Pour into sterile Petri dishes. Prior to inoculation, aseptically spread 0.2mL of sterile formate-fumarate solution on each plate.

Use: For the isolation of Streptococcus pneumoniae from a variety of clinical specimens.

Trypticase™ Soy Agar with Sheep Blood and Gentamicin (TSA II™ with Sheep Blood and Gentamicin) Composition per liter: Pancreatic digest of casein.......................................................... 14.5g Agar ............................................................................................ 14.0g Papaic digest of soybean meal...................................................... 5.0g NaCl.............................................................................................. 5.0g Growth factors .............................................................................. 1.5g Sheep blood, defibrinated ........................................................50.0mL Gentamicin solution.................................................................10.0mL pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

1828

Trypticase™ Soy Agar with Sheep Blood and Streptomycin

Gentamicin Solution: Composition per 10.0mL:

Source: Growth factors are available as a premixed powder from BD

Gentamicin................................................................................. 2.5mg

Tetracycline Solution: Composition per 10.0mL:

Preparation of Gentamicin Solution: Add gentamicin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except sheep blood and gentamicin solution, to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile sheep blood and sterile gentamicin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation of Streptococcus pneumoniae from a variety of clinical specimens.

Trypticase™ Soy Agar with Sheep Blood and Streptomycin (ATCC Medium 1810) Composition per liter: Pancreatic digest of casein .......................................................... 14.5g Agar ............................................................................................ 14.0g Papaic digest of soybean meal ...................................................... 5.0g NaCl .............................................................................................. 5.0g Growth factors .............................................................................. 1.5g Sheep blood, defibrinated ........................................................50.0mL Streptomycin solution ..............................................................10.0mL pH 7.3 ± 0.2 at 25°C

Source: Growth factors are available as a premixed powder from BD Diagnostic Systems.

Streptomycin Solution: Composition per 10.0mL: Streptomycin ................................................................................. 0.5g

Preparation of Streptomycin Solution: Add streptomycin to dis-

Diagnostic Systems.

Tetracycline................................................................................ 0.5mg

Preparation of Tetracycline Solution: Add tetracycline to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except sheep blood and tetracycline solution, to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile sheep blood and sterile tetracycline solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation of Streptococcus pneumoniae from a variety of clinical specimens.

Trypticase™ Soy Agar with Sheep Blood and Tween™ 80 (ATCC Medium 1893) Composition per liter: Pancreatic digest of casein.......................................................... 15.0g Agar ............................................................................................ 15.0g Papaic digest of soybean meal...................................................... 5.0g NaCl.............................................................................................. 5.0g Sheep blood, defibrinated ........................................................50.0mL Tween™ 80..............................................................................10.0mL pH 7.3 ± 0.2 at 25°C

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Use: For the cultivation of a wide variety of fastidious microorganisms.

Preparation of Medium: Add components, except sheep blood and streptomycin solution, to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°– 50°C. Aseptically add sterile sheep blood and sterile streptomycin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Trypticase™ Soy Agar with Sheep Blood and Vancomycin (ATCC Medium 1976)

Use: For the cultivation of Streptococcus gordonii.

Trypticase™ Soy Agar with Sheep Blood, Sucrose, and Tetracycline Composition per liter: Pancreatic digest of casein .......................................................... 14.5g Agar ............................................................................................ 14.0g Papaic digest of soybean meal ...................................................... 5.0g NaCl .............................................................................................. 5.0g Sucrose.......................................................................................... 2.0g Growth factors .............................................................................. 1.5g Sheep blood, defibrinated ........................................................50.0mL Tetracycline solution................................................................10.0mL pH 7.3 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

Composition per liter: Pancreatic digest of casein.......................................................... 14.5g Agar ............................................................................................ 14.0g Papaic digest of soybean meal...................................................... 5.0g NaCl.............................................................................................. 5.0g Growth factors .............................................................................. 1.5g Sheep blood, defibrinated ........................................................50.0mL Vancomycin solution ...............................................................10.0mL pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Vancomycin Solution: Composition per 10.0mL: Vancomycin ............................................................................... 4.0mg

Preparation of Vancomycin Solution: Add vancomycin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Trypticase™ Soy Agar with Yeast Extract

1829

Preparation of Medium: Add components, except sheep blood and vancomycin solution, to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add sterile sheep blood and sterile vancomycin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Preparation of Medium: Add components, except horse serum

Use: For the isolation of Streptococcus and Enterococcus spp. from a

Use: For the isolation and cultivation of fungi.

variety of clinical specimens.

Trypticase™ Soy Agar with Sodium Chloride (ATCC Medium 176) Composition per liter: NaCl ............................................................................................ 30.0g Pancreatic digest of casein .......................................................... 15.0g Agar ............................................................................................ 15.0g Papaic digest of soybean meal ...................................................... 5.0g Bile salts No. 3.............................................................................. 1.0g pH 7.3 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Vibrio alginolyticus.

Trypticase™ Soy Agar with 3% Sodium Chloride (TSA NaCl) Composition per liter: NaCl ............................................................................................ 30.0g Agar ............................................................................................ 15.0g Pancreatic digest of casein .......................................................... 15.0g Papaic digest of soybean meal ...................................................... 5.0g pH 7.3 ± 0.2 at 25°C

Use: For the cultivation of halophilic microorganisms isolated from foods.

Trypticase™ Soy Agar with Sodium Chloride, Horse Serum, and Penicillin Composition per liter: NaCl ............................................................................................ 35.0g Pancreatic digest of casein .......................................................... 15.0g Agar ............................................................................................ 15.0g Papaic digest of soybean meal ...................................................... 5.0g Horse serum, inactivated........................................................100.0mL Penicillin solution ....................................................................10.0mL pH 7.3 ± 0.2 at 25°C

Penicillin Solution: Composition per 10.0mL: Penicillin ........................................................................... 1,000,000U

and penicillin solution, to distilled/deionized water and bring volume to 890.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Do not overheat. Cool to 50°C. Aseptically add 100.0mL of sterile horse serum and 10.0mL of sterile penicillin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Trypticase™ Soy Agar with Starch (ATCC Medium 1818) Composition per liter: Pancreatic digest of casein.......................................................... 15.0g Agar ............................................................................................ 15.0g Starch, soluble............................................................................. 10.0g Papaic digest of soybean meal...................................................... 5.0g NaCl.............................................................................................. 5.0g pH 5.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.5. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Bacillus naganoensis.

Trypticase™ Soy Agar with Tobramycin Composition per liter: Pancreatic digest of casein.......................................................... 17.0g Agar ............................................................................................ 15.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Tobramycin solution ................................................................10.0mL pH 7.3 ± 0.2 at 25°C

Tobromycin Solution: Composition per 10.0mL: Tobramycin ................................................................................ 8.0mg

Preparation of Tobromycin Solution: Add tobramycin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except tobramycin solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile tobramycin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Serratia marcescens.

Trypticase™ Soy Agar with Yeast Extract (TSAYE) Composition per liter: Pancreatic digest of casein.......................................................... 17.0g Agar ............................................................................................ 15.0g Yeast extract.................................................................................. 6.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g

1830

Trypticase™ Soy Agar with Yeast Extract and Glucose

K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 2.5g pH 7.3 ± 0.2 at 25°C

Use: For the cultivation and maintenance of a wide variety of heterotrophic microorganisms. For the isolation and cultivation of Listeria monocytogenes from foods.

Trypticase™ Soy Agar with Yeast Extract and Glucose Composition per liter: Pancreatic digest of casein .......................................................... 17.0g Agar ............................................................................................ 15.0g Glucose ......................................................................................... 7.5g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 3.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g pH 7.0–7.2 at 25°C

Use: For the cultivation and maintenance of Pediococcus urinaeequi.

Trypticase™ Soy Broth (Soybean Casein Digest Broth, USP) (LMG Medium 185) Composition per liter: Pancreatic digest of casein .......................................................... 17.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 2.5g pH 7.3 ± 0.2 at 25°C

Pancreatic digest of casein.......................................................... 17.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Agar .............................................................................................. 1.0g pH 7.3 ± 0.2 at 25°C

Use: For the cultivation of anaerobic microorganisms. For the cultivation of microorganisms isolated from root canals and other clinical specimens.

Trypticase™ Soy Broth Agar Modified (DSMZ Medium 535a) Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein.......................................................... 17.0g NaCl.............................................................................................. 5.0g Pancreatic digest of soybean meal................................................ 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Horse blood, defibrinated ........................................................50.0mL pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except horse blood, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Heat with frequent agitation and boil for 1 min to completely dissolve. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°– 50°C. Aseptically add 50.0mL of sterile, defibrinated horse blood. Mix thoroughly and pour into sterile Petri dishes. Use: For cultivation and maintenance of Corynebacterium glucuronolyticum, Corynebacterium durum, Corynebacterium falsenii, Corynebacterium singulare, and Corynebacterium xerosis.

Trypticase™ Soy Broth with Calcium Chloride

Source: This medium is available as a premixed powder from BD Di-

Composition per liter:

agnostic Systems.

Pancreatic digest of casein.......................................................... 17.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g CaCl2·2H2O ................................................................................ 0.15g pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Bordetella spp., Capnocytophaga spp., Brachybacterium spp., Erwinia amylovora, Curtobacterium flaccumfaciens, Aeromonas spp., Ralstonia spp., Ornithobacterium rhinotracheale, Riemerella spp., Arthrobacter spp., Pedobacter spp., Burkholderia vietnamiensis, Myroides spp., Chryseobacterium spp., Microbacterium spp., Nocardioides spp., Cellulomonas spp., Agromyces spp., Pelistega europaea, Bergeyella zoohelcum, Bacillus spp., Burkholderia spp., Brevibacillus spp., Aneurinibacillus aneurinilyticus, Pandoraea spp., and Pseudomonas spp. For the cultivation of a wide variety of fastidious and nonfastidious microorganisms from clinical and nonclinical specimens. Also used for the rapid estimation of the bacteriological quality of water.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Brochothrix thermosphacta.

Trypticase™ Soy Broth with Cefazidime Composition per liter: Pancreatic digest of casein.......................................................... 17.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g

Trypticase™ Soy Broth with Horse Serum

Glucose ......................................................................................... 2.5g Ceftazidime solution ................................................................10.0mL pH 7.3 ± 0.2 at 25°C

Ceftazidime Solution: Composition per 10.0mL: Ceftazidime ................................................................................ 4.0mg

Preparation of Ceftazidime Solution: Add ceftazidime to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except ceftazidime solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 10.0mL of sterile ceftazidime solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivaion of Klebsiella oxytoca.

Trypticase™ Soy Broth with Ferrous Sulfate (Tryptic Soy Broth with Ferrous Sulfate) (BAM M186) Composition per liter:

1831

Preparation of Medium: Add components, except fetal calf serum, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add sterile fetal calf serum. Mix thoroughly. Aseptically distribute into sterile tubes.

Use: For the cultivation and maintenance of Serpula innocens and Brachyspira innocens.

Trypticase™ Soy Broth with 10mM Glucose (ATCC Medium 1189) Composition per liter: Pancreatic digest of casein.......................................................... 18.0g Papaic digest of soybean meal...................................................... 6.0g NaCl.............................................................................................. 6.0g Glucose ......................................................................................... 1.0g pH 7.3 ± 0.2 at 25°C

Source: This medium, without glucose, is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Do not overheat. Pour into sterile Petri dishes or leave in tubes.

Pancreatic digest of casein .......................................................... 17.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ........................................................................................ 2.5g Glucose ......................................................................................... 2.5g FeSO4 ....................................................................................... 35.0mg pH 7.3 ± 0.2 at 25°C

Use: For the isolation and cultivation of Staphylococcus aureus subsp. aureus.

Preparation of Medium: Add components to distilled/deionized

Composition per liter:

water and bring volume to 1.0L. Mix thoroughly. Gently heat until dissolved. Adjust pH to 7.3. Distribute into tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the detection of Salmonella spp. from foods.

Trypticase™ Soy Broth with 10mM Glucose

Trypticase™ Soy Broth with Glycerol Pancreatic digest of casein.......................................................... 17.0g NaCl............................................................................................ 15.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4 ........................................................................................ 2.5g Glycerol .................................................................................240.0mL pH 7.3 ± 0.2 at 25°C

Composition per liter:

Preparation of Medium: Add components to distilled/deionized

Pancreatic digest of casein .......................................................... 17.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 1.8g pH 7.3 ± 0.2 at 25°C

Use: For the cultivation and maintenance of a wide variety of micro-

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of a variety of fastidious and nonfastidious microorganisms from clinical and nonclinical specimens.

Trypticase™ Soy Broth with Fetal Calf Serum Composition per liter: Pancreatic digest of casein .......................................................... 17.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Fetal calf serum......................................................................100.0mL pH 7.3 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

water and bring volume to 1.0L. Mix thoroughly. Gently heat until dissolved. Adjust pH to 7.3. Distribute into tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. organisms from foods.

Trypticase™ Soy Broth with Horse Serum Composition per liter: Pancreatic digest of casein.......................................................... 17.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Horse serum, inactivated .......................................................100.0mL pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except horse serum, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 100.0mL of sterile horse serum. Mix thoroughly. Aseptically distribute into sterile tubes. Use: For the cultivation and maintenance of Serpula innocens.

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Trypticase™ Soy Broth with Human Blood

Trypticase™ Soy Broth with Human Blood Composition per liter: Pancreatic digest of casein .......................................................... 17.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Human blood, defibrinated ......................................................50.0mL pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except human blood, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 50.0mL of sterile human blood. Mix thoroughly. Aseptically distribute into sterile tubes.

Use: For the cultivation and maintenance of Serpula innocens.

Trypticase™ Soy Broth with 0.1% DL-7-Hydroxybutyric Acid (LMG Medium 266) Composition per liter: Pancreatic digest of casein .......................................................... 17.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 2.5g DL-7-Hydroxybutyric acid ............................................................ 1.0g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Gently warm with mixing until solution is complete. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For cultivation and maintenance of Paucimonas lemoignei.

Trypticase™ Soy Broth, Modified Composition per 1000.2mL: Pancreatic digest of casein .......................................................... 17.0g NaCl ............................................................................................ 15.0g K2HPO4 ........................................................................................ 4.0g Papaic digest of soybean meal ...................................................... 3.0g Glucose ......................................................................................... 2.5g Bile salts No. 3.............................................................................. 1.5g Novobiocin solution...................................................................0.2mL pH 7.3 ± 0.2 at 25°C

Trypticase™ Soy Broth, Modified (mTSB) (BAM M156) Composition per 1000.2mL: Pancreatic digest of casein.......................................................... 17.0g NaCl.............................................................................................. 5.0g K2HPO4 ........................................................................................ 4.0g Papaic digest of soybean meal...................................................... 3.0g Glucose ......................................................................................... 2.5g Bile salts No. 3.............................................................................. 1.5g Novobiocin solution...................................................................0.2mL pH 7.3 ± 0.2 at 25°C

Novobiocin Solution: Composition per liter: Novobiocin ................................................................................. 0.05g

Preparation of Novobiocin Solution: Add novobiocin to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except novobiocin solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile novobiocin solution. Mix thoroughly. Aseptically distribute into sterile tubes.

Use: For the isolation and cultivation of Shigella species from food.

Trypticase™ Soy Broth with Neomycin Composition per liter: Pancreatic digest of casein.......................................................... 17.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Agar .............................................................................................. 1.0g Neomycin solution...................................................................10.0mL pH 7.3 ± 0.2 at 25°C

Neomycin Solution: Composition per 10.0mL: Neomycin................................................................................... 5.0mg

Preparation of Neomycin Solution: Add neomycin to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except neomycin solu-

Novobiocin.................................................................................. 0.05g

tion, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile neomycin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Preparation of Novobiocin Solution: Add novobiocin to dis-

Use: For the cultivation and maintenance of Bacillus megaterium.

Novobiocin Solution: Composition per liter:

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Trypticase™ Soy Broth with 1M Potassium Chloride (LMG Medium 256) Composition per liter: KCl.............................................................................................. 74.5g Pancreatic digest of casein.......................................................... 17.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g

Trypticase™ Soy Broth with Tobramycin

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Gracilibacillus dipsosauri.

Trypticase™ Soy Broth with Sea Salts (LMG Medium 249) Composition per liter: Sea salts....................................................................................... 20.0g Pancreatic digest of casein .......................................................... 17.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Yeast extract.................................................................................. 1.0g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Cellulophaga baltica and Cellulophaga fucicola.

Trypticase™ Soy Broth with Sheep Blood (LMG Medium 189) Composition per liter: Pancreatic digest of casein .......................................................... 17.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Sheep blood, defibrinated ........................................................50.0mL

Preparation of Medium: Add components, except sheep blood, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile, defibrinated sheep blood. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Bartonella bacilliformis.

Trypticase™ Soy Broth with 1.5% Sodium Chloride Composition per liter: Pancreatic digest of casein .......................................................... 17.0g NaCl ............................................................................................ 15.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 2.5g pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

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Trypticase™ Soy Broth with Sodium Chloride and Sodium Pyruvate Composition per liter: NaCl.......................................................................................... 100.0g Pancreatic digest of casein.......................................................... 17.0g Sodium pyruvate......................................................................... 10.0g Papaic digest of soybean meal...................................................... 3.0g Glucose ......................................................................................... 2.5g K2HPO4......................................................................................... 2.5g pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until dissolved. Adjust pH to 7.3. Distribute into tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of Staphylococcus aureus from foods.

Trypticase™ Soy Broth with Starch (LMG Medium 232) Composition per liter: Pancreatic digest of casein.......................................................... 17.0g Starch, soluble............................................................................. 10.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Bacillus naganoensis.

Trypticase™ Soy Broth with Tobramycin Composition per liter: Pancreatic digest of casein.......................................................... 17.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Agar .............................................................................................. 1.0g Tobramycin solution ................................................................10.0mL pH 7.3 ± 0.2 at 25°C

Tobramycin Solution: Composition per 10.0mL: Tobramycin ................................................................................ 8.0mg

Preparation of Tobramycin Solution: Add tobramycin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except tobramycin solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile tobramycin solution. Mix thoroughly. Aseptically distribute into sterile tubes.

Use: For the cultivation and maintenance of Serratia marcescens.

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Trypticase™ Soy Broth with Tween™ 80

Trypticase™ Soy Broth with Tween™ 80 Composition per liter: Pancreatic digest of casein .......................................................... 17.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Agar .............................................................................................. 1.0g Tween™ 80 ................................................................................1.0mL pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Corynebacterium genitalium.

Trypticase™ Soy Broth with Yeast Extract Composition per liter: Pancreatic digest of casein .......................................................... 17.0g Yeast extract.................................................................................. 6.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 2.5g pH 7.3 ± 0.2 at 25°C

Use: For the cultivation and maintenance of a wide variety of heterotrophic microorganisms.

Trypticase™ Soy Broth with 0.1% Yeast Extract (LMG Medium 235) Composition per liter: Pancreatic digest of casein .......................................................... 17.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Yeast extract.................................................................................. 1.0g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For teh cultivation and maintenance of Bacillus spp. and other heterotrophic bacteria.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of a wide variety of microorganisms when the presence of carbohydrate is undesirable.

Trypticase™ Soy Broth with Yeast Extract (TSBYE) Composition per liter: Pancreatic digest of casein.......................................................... 17.0g Yeast extract.................................................................................. 6.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g pH 7.3 ± 0.2 at 25°C

Use: For the cultivation of Listeria monocytogenes from foods.

Trypticase™ Soy Glucose Medium Composition per liter: Glucose ....................................................................................... 50.0g Pancreatic digest of casein............................................................ 7.5g Agar .............................................................................................. 7.5g Papaic digest of soybean meal...................................................... 2.5g NaCl.............................................................................................. 2.5g pH 7.3 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Pseudomonas cepacia.

Trypticase™ Soy Polymyxin Broth Composition per 1006.67mL: Pancreatic digest of casein.......................................................... 17.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Polymyxin B solution ..............................................................6.67mL pH 7.3 ± 0.2 at 25°C

Polymyxin B Solution: Composition per 10.0mL: Polymyxin B ............................................................................. 0.015g

Trypticase Soy Broth without Glucose Composition per liter: Pancreatic digest of casein .......................................................... 17.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g pH 7.3 ± 0.2 at 25°C

Preparation of Polymyxin B Solution: Add polymyxin B to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except polymyxin B solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 15.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 0.1mL of sterile polymyxin B solution to each tube. Mix thoroughly.

Trypticase™ Soy Yeast Extract Agar Use: For the isolation and cultivation of Bacillus cereus from foods.

Trypticase™ Soy Sheep Blood Agar (Tryptic Soy Blood Agar) (TSA Blood Agar) (BAM M159)

Fe-citrate solution ......................................................................5.0mL Trace elements solution .............................................................5.0mL

Fe-Citrate Solution: Composition per 100.0mL: Fe-citrate ..................................................................................... 0.25g

Preparation of Fe-Citrate Solution: Add Fe-citrate to distilled/

Composition per 1050.0mL:

deionized water and bring volume to 100.0mL. Mix thoroughly.

Trace Elements Solution: Composition per 5.0mL:

Preparation of Medium: Add components, except sheep blood, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL sterile sheep blood. Mix thoroughly. Pour into sterile Petri dishes in 20.0mL volumes or distribute into sterile tubes. Use: For the cultivation of a wide variety of fastidious microorganisms. For the observation of hemolytic reactions of a variety of bacteria. May be used to perform the CAMP test for the presumptive identification of group B streptococci (Streptococcus agalactiae).

Trypticase™ Soy Soil Extract See: TS Soil Extract

Trypticase™ Soy Tryptose Broth Composition per liter: Pancreatic digest of casein .......................................................... 13.5g Peptic digest of animal tissue........................................................ 5.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 3.0g Glucose ....................................................................................... 1.75g Papaic digest of soybean meal ...................................................... 1.5g K2HPO4 ....................................................................................... 1.25g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes in 5.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the enrichment of Salmonella species from foods.

Trypticase™ Soy Yeast Extract Agar Composition per liter: Agar ............................................................................................ 28.0g NaCl ............................................................................................ 10.0g Pancreatic digest of casein ............................................................ 2.5g Yeast extract.................................................................................. 2.5g Base medium..........................................................................100.0mL Phosphate buffer ....................................................................100.0mL pH 7.2 ± 0.2 at 25°C

Base Medium: Composition per liter: MgCl2·6H2O ................................................................................. 2.0g Titriplex I ...................................................................................... 1.0g CaSO4·2H2O ................................................................................. 0.4g NaOH pellets................................................................................. 0.2g © 2010 by Taylor and Francis Group, LLC

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CuCl2·2H2O............................................................................. 50.0mg Titriplex I ................................................................................. 12.8mg FeCl2·4H2O................................................................................ 1.0mg MnCl2·4H2O .............................................................................. 0.5mg CoCl2·6H2O............................................................................... 0.3mg ZnCl2 ......................................................................................... 0.2mg Na2MoO4·2H2O....................................................................... 50.0mg NiCl2·6H2O.............................................................................. 50.0mg H3BO3 ...................................................................................... 20.0mg

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 5.0mL. Mix thoroughly. Preparation of Base Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Phosphate Buffer: Composition per liter: Na2HPO4·12H2O ........................................................................ 43.0g KH2PO4 ...................................................................................... 5.44g

Preparation of Phosphate Buffer: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.

Preparation of Medium: Add components, except phosphate buffer, and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 100.0mL of sterile phosphate buffer. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Lactococcus lactis and Lactococcus plantarum.

Trypticase™ Soy Yeast Extract Agar Composition per liter: Pancreatic digest of casein.......................................................... 17.0g Agar ............................................................................................ 15.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 3.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g pH 7.1 ± 0.2 at 25°C

Use: For the cultivation of Staphylococcus carnosus. Trypticase™ Soy Yeast Extract Medium See: TSY Medium

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Trypticase™ Soy Yeast Extract Medium

Trypticase™ Soy Yeast Extract Medium (LMG Medium 217) Composition per liter: Pancreatic digest of casein .......................................................... 17.0g NaCl .............................................................................................. 8.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Sheep blood, defibrinated ........................................................50.0mL

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Staphylococcus carnosus and Brachybacterium spp.

Trypticase™ Soy Yeast Extract Starch Medium See: TSYES Medium

Trypticase™ Starch Agar (DSMZ Medium 56) Composition per liter: Pancreatic digest of casein .......................................................... 17.0g Agar ............................................................................................ 15.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Yeast extract.................................................................................. 2.0g Soluble starch................................................................................ 1.0g pH 7.3 ± 0.2 at 25°C

Caution: Potassium tellurite is toxic. Preparation of Medium: Add components, except serum and Chapman tellurite solution, to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile serum and sterile Chapman tellurite solution. Sheep serum, rabbit serum, or human serum may be used. Mix thoroughly. Pour into sterile Petri dishes.

Use: For the selective isolation of microorganisms from clinical specimens, especially from the nose, throat, and vagina.

Trypticase™ Yeast Extract Glucose Medium Composition per liter: Pancreatic digest of casein.......................................................... 10.0g Glucose ....................................................................................... 10.0g KH2PO4......................................................................................... 6.8g Yeast extract.................................................................................. 5.0g NaHCO3 ........................................................................................ 1.0g Tween™ 80................................................................................... 0.5g Sodium formaldehyde sulfoxalate ................................................ 0.5g CaCl2 ........................................................................................... 0.02g MgSO4 ........................................................................................ 0.02g NaCl............................................................................................ 0.02g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation of Propionibacterium species. Trypticase™ Yeast Extract Glucose Medium See: TYEG Medium

Preparation of Medium: Add components to distilled/deionized

Trypticase™ Yeast Extract Glucose Medium See: TYG Medium

Use: For the cultivation and maintenance of Bacillus spp. Trypticase™ Sulfite Neomycin Agar See: TSN Agar

Trypticase™ Tellurite Agar Base Composition per liter: Agar ............................................................................................ 20.0g Pancreatic digest of casein .......................................................... 10.0g Peptic digest of animal tissue...................................................... 10.0g NaCl .............................................................................................. 5.0g Glucose ......................................................................................... 2.0g Serum .......................................................................................50.0mL Chapman tellurite solution.......................................................10.0mL pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Tryptone Agar Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein............................................................ 8.0g NaCl.............................................................................................. 8.0g pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of fastidious aerobic and facultative microorganisms such as Escherichia coli and Pseudomonas species.

Tryptone Agar Base, HiVeg Composition per liter:

Chapman Tellurite Solution: Composition per 100.0mL:

Plant hydrolysate ........................................................................ 20.0g Agar .............................................................................................. 3.5g Phenol Red.................................................................................. 0.02g pH 7.2 ± 0.2 at 25°C

K2TeO3 .......................................................................................... 1.0g

Source: This medium is available as a premixed powder from Hi-

Preparation of Chapman Tellurite Solution: Add K2TeO3 to

Media.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

Tryptone Broth

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of fastidious aerobic and facultative microorganisms such as Escherichia coli and Pseudomonas species.

Tryptone Agar, HiVeg Composition per liter: Plant hydrolysate......................................................................... 20.0g Agar ............................................................................................ 15.0g Synthetic detergent No. 1.............................................................. 1.5g pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Use: For the cultivation and maintenance of fastidious aerobic and facultative microorganisms such as Escherichia coli and Pseudomonas species.

Tryptone Beef Yeast Extract Acetate Agar See: TBYA Agar

Tryptone Bile Agar Composition per liter: Pancreatic digest of casein .......................................................... 20.0g Agar ............................................................................................ 15.0g Bile salts No. 3.............................................................................. 1.5g pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems and Oxoid Unipath.

Use: For the isolation and enumeration of Escherichia coli from foods.

Tryptone Bile Glucuronide Agar, Harlequin (Harlequin TBGA) Composition per liter: Tryptone ...................................................................................... 20.0g Agar ............................................................................................ 15.0g Bile salts No. 3.............................................................................. 1.5g X-glucuronide ........................................................................... 0.075g pH 7.2 ± 0.2 at 25°C

Source: This medium is available from lab m. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Allow to soak for 10 min. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°– 50°C. Pour into sterile Petri dishes.

Use: For the simple enumeration of E. coli without the need for membranes or pre-incubation The medium has been modified by the addition of a chromogenic substrate to detect the β-glucuronidase, which is © 2010 by Taylor and Francis Group, LLC

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highly specific for E. coli. The advantage of the chromogenic substrate is that it requires no UV lamp to visualize the reaction, and it is concentrated within the colony, facilitating easier enumeration in the presence of other organisms, or when large numbers are present on the plate.

Tryptone Bile X-glucuronide Agar, Chromocult (Chromocult® TBX ) (ChromocultTryptone Bile X-glucuronide Agar) Composition per liter: Peptone ...................................................................................... 20.0g Agar ............................................................................................ 15.0g Bile salts No. 3 ............................................................................. 1.5g X-β-D-glucuronide ................................................................... 0.075g pH 7.2 ± 0.2 at 25°C

Source: This medium is available from Merck. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Pour into sterile Petri dishes.

Use: For the differentiation of E. coli from other coliforms. The pres-

ence of the enzyme β-D-glucuronidase differentiates most E. coli spp. from other coliforms. E. coli absorbs the chromogenic substrate 5bromo-4-chloro-3-indolyl-β-D-glucuronide (X-β-D-glucuronide). The enzyme β-glucuronidase splits the bond between the chromophore 5bromo-4-chloro-3-indolyle and the β-D-glucuronide. E. coli colonies are colored blue-green. Growth of accompanying Gram-positive flora is largely inhibited by the use of bile salts. The prepared medium is clear and yellowish.

Tryptone Broth Composition per liter: Pancreatic digest of casein.......................................................... 10.0g Glucose ......................................................................................... 5.0g K2HPO4....................................................................................... 1.25g Yeast extract.................................................................................. 1.0g Bromcresol Purple solution .......................................................2.0mL

Bromcresol Purple Solution: Composition per 100.0mL: Bromcresol Purple ........................................................................ 2.0g Ethanol.....................................................................................10.0mL

Preparation of Bromcresol Purple Solution: Add Bromcresol Purple to 10.0mL of ethanol. Mix thoroughly. Bring volume to 100.0mL with distilled/deionized water. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into screwcapped tubes in 10.0mL volumes. Autoclave for 20 min at 15 psi pressure–121°C.

Use: For the cultivation of Salmonella species from foods. Tryptone Broth See: T1N0 Broth Tryptone Broth See: Tryptone Water Broth

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Tryptone Broth

Tryptone Broth (ATCC Medium 274) Composition per liter:

Pancreatic digest of casein .......................................................... 10.0g pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di-

Preparation of Medium: Add components to distilled/deionized

Use: For the cultivation and maintenance of fastidious aerobic and facultative microorganisms such as Escherichia coli and Pseudomonas species.

Tryptone Broth, 1% (ATCC Medium 274) Composition per liter: Pancreatic digest of casein ............................................................ 1.0g pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Escherichia coli and Pseudomonas species. For the identification and confirmation of Vibrio cholerae in foods.

Tryptone Broth with CaCl2

Composition per liter:

Pancreatic digest of casein .......................................................... 10.0g CaCl2 ............................................................................................. 5.5g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of fastidious aerobic and facultative microorganisms such as Escherichia coli.

Tryptone Broth, HiVeg (Tryptone Water, HiVeg)

agnostic Systems and Oxoid Unipath. water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. If the dilution of the specimen is greater than 1:10, add 10.0mL of sterile 10% skim milk solution. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the enumeration of bacteria by the standard plate count procedure. For the cultivation and enumeration of bacteria from milk and dairy products. For the detection of thermophilic organisms.

Tryptone Glucose Beef Extract Agar with Sucrose Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein............................................................ 5.0g Beef extract................................................................................... 3.0g Glucose ......................................................................................... 1.0g Sucrose.......................................................................................... 5.0g pH 7.0 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Saccharococcus thermophilus.

Tryptone Glucose Beef Extract Agar with Yeast Extract Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein............................................................ 5.0g Beef extract................................................................................... 3.0g Glucose ......................................................................................... 1.0g Yeast extract.................................................................................. 1.0g pH 7.0 ± 0.2 at 25°C

Composition per liter:

Preparation of Medium: Add components to distilled/deionized

Plant hydrolysate......................................................................... 10.0g NaCl .............................................................................................. 5.0g pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-

Use: For the cultivation and maintenance of Ancylobacter aquaticus and Spirosoma linguale.

Media.

Use: For the detection of indole-producing microorganisms.

Tryptone Glucose Beef Extract Agar (Tryptone Glucose Extract Agar) Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein ............................................................ 5.0g © 2010 by Taylor and Francis Group, LLC

Tryptone Glucose Extract Agar See: Tryptone Glucose Beef Extract Agar

Tryptone Glucose Extract HiVeg Agar (Tryptone Glucose Yeast Extract HiVeg Agar) Composition per liter: Agar ............................................................................................ 15.0g Plant hydrolysate .......................................................................... 5.0g Yeast extract.................................................................................. 3.0g Glucose ......................................................................................... 1.0g pH 7.0 ± 0.2 at 25°C

Tryptone Lactose Iron HiVeg Agar Source: This medium is available as a premixed powder from HiMedia.

Preparation of Medium: Add components to distilled/deionized

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K2HPO4....................................................................................... 1.25g Yeast extract.................................................................................. 1.0g pH 6.8 ± 0.2 at 25°C

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Source: This medium is available as a premixed powder from Hi-

Use: For the enumeration of bacteria by the standard plate count proce-

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

dure. For the cultivation and enumeration of bacteria from milk and dairy products.

Tryptone Glucose HiVeg Agar Composition per liter: Plant hydrolysate......................................................................... 20.0g Glucose ......................................................................................... 5.0g Agar .............................................................................................. 3.5g Bromthymol Blue ....................................................................... 0.01g pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Use: For the enumeration of bacteria by the standard plate count proce-

Media.

Preparation of Medium: Add components to distilled/deionized

Use: For the enumeration of bacteria by the standard plate count procedure. For the cultivation and enumeration of bacteria from milk and dairy products.

Tryptone with Sodium Chloride Broth Composition per liter: Pancreatic digest of casein............................................................ 8.0g NaCl.............................................................................................. 0.5g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of fastidious aerobic and facultative microorganisms such as Escherichia coli and Pseudomonas species.

dure. For the cultivation and enumeration of bacteria from milk and dairy products.

Tryptone Glucose Yeast Agar See: Plate Count Agar

Tryptone Glucose Medium

Tryptone Glucose Yeast Agar See: Standard Methods Agar

Composition per liter: Pancreatic digest of casein .......................................................... 20.0g Glucose ......................................................................................... 5.0g K2HPO4 ....................................................................................... 0.35g KH2PO4 ......................................................................................... 0.2g MgCl2·6H2O.................................................................................. 0.1g CaCl2·2H2O................................................................................. 0.07g Vitamin solution.......................................................................10.0mL

Vitamin Solution: Composition per 10.0mL: Thiamine .................................................................................200.0μg Biotin ........................................................................................50.0μg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except vitamin solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0mL of sterile vitamin solution. Mix thoroughly. Aseptically distribute into sterile flasks or tubes.

Tryptone Glucose Yeast Broth See: Standard Methods Broth Tryptone Glucose Yeast Extract Medium See: TGY Medium Tryptone Glucose Yeast Extract Methionine Medium See: TGYM Medium

Tryptone Lactose Iron HiVeg Agar Composition per liter: Plant hydrolysate ........................................................................ 20.0g Lactose........................................................................................ 10.0g Agar .............................................................................................. 3.5g Na2SO3 ......................................................................................... 0.4g FeSO4 ............................................................................................ 0.2g Na2S2O3 ...................................................................................... 0.08g Phenol Red.................................................................................. 0.02g pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-

Use: For the cultivation and maintenance of Amoebidium parasiticum,

Media.

Capniomyces stellatus, Smittium culicis, Smittium culisetae, Smittium simulii, and Smittium species.

Preparation of Medium: Add components to distilled/deionized

Tryptone Glucose Yeast Extract HiVeg Broth Composition per liter: Plant hydrolysate......................................................................... 10.0g Glucose ......................................................................................... 5.0g © 2010 by Taylor and Francis Group, LLC

Use: For the cultivation and maintenance of fastidious aerobic and facultative microorganisms such as Escherichia coli and Pseudomonas species.

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Tryptone Nitrate HiVeg Medium

Tryptone NaCl Thiamine Medium See: TNT Medium

Tryptone Nitrate HiVeg Medium (Indole Nitrate HiVeg Medium) Composition per liter: Plant peptone............................................................................... 20.0g Na2HPO4 ....................................................................................... 2.0g Agar .............................................................................................. 1.0g Glucose ......................................................................................... 1.0g KNO3 ............................................................................................ 1.0g pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling with frequent agitation. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the identification of microorganisms by means of the nitrate reduction and indole tests.

Tryptone Peptone Glucose Yeast Extract HiVeg Broth Base without Trypsin Composition per liter: Plant hydrolysate......................................................................... 50.0g Yeast extract................................................................................ 20.0g Plant peptone................................................................................. 5.0g Glucose ......................................................................................... 4.0g Na-thioglycolate............................................................................ 1.0g pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Use: For the enumeration of bacteria by the standard plate count procedure. For the cultivation and enumeration of bacteria from milk and dairy products. For the detection of thermophilic organisms.

Tryptone Phosphate Brain Heart Infusion Yeast Extract Agar (TPBY) Composition per liter: Pancreatic digest of casein .......................................................... 20.0g Agar ............................................................................................ 15.0g NaCl ............................................................................................ 5.14g K2HPO4 ......................................................................................... 2.0g KH2PO4 ......................................................................................... 2.0g Yeast extract.................................................................................. 1.0g Oxgall............................................................................................ 0.5g Pancreatic digest of gelatin ........................................................... 0.4g Brain heart, solids from infusion ................................................ 0.16g Peptic digest of animal tissue...................................................... 0.16g Glucose ....................................................................................... 0.08g Na2HPO4 ..................................................................................... 0.06g Tween™ 80 ................................................................................1.5mL pH 7.0 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of coliform bacteria, such as Escherichia coli, from foods.

Tryptone Phosphate Broth Composition per liter: Pancreatic digest of casein.......................................................... 20.0g NaCl.............................................................................................. 5.0g K2HPO4 ........................................................................................ 2.0g KH2PO4 ........................................................................................ 2.0g Tween™ 80..............................................................................15.0mL pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of enteropathogenic Escherichia coli.

Tryptone Phosphate Broth Composition per liter: Pancreatic digest of casein.......................................................... 20.0g NaCl.............................................................................................. 5.0g K2HPO4......................................................................................... 2.0g KH2PO4......................................................................................... 2.0g Tween™ 80................................................................................1.5mL pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of coliform bacteria, such as Escherichia coli, from foods.

Tryptone Phosphate HiVeg Broth Composition per liter: Plant hydrolysate ........................................................................ 20.0g NaCl.............................................................................................. 5.0g K2HPO4......................................................................................... 2.0g KH2PO4......................................................................................... 2.0g Polysorbate 80 .............................................................................. 1.5g pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of enteropathogenic Escherichia coli. For the enrichment and cultivation of enteropathogenic Escherichia coli from suspected food samples.

Tryptone Salt Agar See: T1N1 Agar Tryptone Salt Agar See: T1N2 Agar

Tryptone Soy Agar with Horse Blood

Tryptone Soy Agar pH 6.5 (LMG Medium 242)

Tryptone Salt Broth See: T1N1 Broth Tryptone Salt Broth See: T1N3 Broth Tryptone Salt Broth See: T1N6 Broth Tryptone Salt Broth See: T1N8 Broth Tryptone Salt Broth See: T1N10 Broth

Tryptone in Seawater Agar

Composition per liter: Tryptone...................................................................................... 15.0g Agar ............................................................................................ 15.0g Soy peptone .................................................................................. 5.0g NaCl.............................................................................................. 5.0g pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.5. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of heterotrophic bacteria.

Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein ............................................................ 1.0g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to seawater and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Bacillus pacificus.

Tryptone Soy Agar Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein .......................................................... 15.0g NaCl .............................................................................................. 5.0g Pancreatic digest of soybean meal ................................................ 5.0g pH 7.3 ± 0.2 at 25°C

Tryptone Soy Agar, HiVeg (Antibiotic Assay Medium - J, HiVeg) Composition per liter: Agar ............................................................................................ 15.0g Plant hydrolysate ........................................................................ 15.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 5.0g pH 7.3 ± 0.1 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Use: A general purpose medium for cultivating a wide variety of fastidious microorganisms.

Source: This medium is available as a premixed powder from Oxoid

Tryptone Soy Agar with Horse Blood (LMG Medium 50)

Unipath.

Use: For the cultivation and maintenance of a wide variety of microorganisms.

Tryptone Soy Agar pH 5.5 (LMG Medium 241) Composition per liter: Tryptone ...................................................................................... 15.0g Agar ............................................................................................ 15.0g Soy peptone................................................................................... 5.0g NaCl .............................................................................................. 5.0g pH 5.5 ± 0.2 at 25°C

1841

Preparation of Medium: Add components, except horse blood, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL sterile horse blood. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Paenibacillus lentimorbus.

Tryptone Soy Agar with Horse Blood Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein.......................................................... 15.0g NaCl.............................................................................................. 5.0g

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Tryptone Soy Agar with 1% Sodium Chloride

Pancreatic digest of soybean meal ................................................ 5.0g Horse blood, defibrinated ........................................................50.0mL pH 7.3 ± 0.2 at 25°C

Tryptone Soy Agar with 1% Sodium Chloride Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein .......................................................... 15.0g NaCl ............................................................................................ 15.0g Pancreatic digest of soybean meal ................................................ 5.0g Cocarboxylase solution..........................................................100.0mL pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except sheep blood, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Heat with frequent agitation and boil for 1 min to completely dissolve. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°– 50°C. Aseptically add 50.0mL of sterile, defibrinated sheep blood. Mix thoroughly and pour into sterile Petri dishes.

Use: For the cultivation and maintenance of Bacillus spp.

Tryptone Soy Broth Composition per liter: Pancreatic digest of casein.......................................................... 17.0g NaCl.............................................................................................. 5.0g Pancreatic digest of soybean meal................................................ 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath.

Preparation of Medium: Add components to distilled/deionized

Cocarboxylase Solution: Composition per 100.0mL:

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Cocarboxylase............................................................................ 2.0mg

Use: For the cultivation of a wide variety of microorganisms.

Preparation of Cocarboxylase Solution: Add cocarboxylase to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except cocarboxylase solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 100.0mL of sterile cocarboxylase solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation and maintenance of Haemophilus piscium.

Tryptone Soy Agar with 4.5% Sodium Chloride Composition per liter: NaCl ............................................................................................ 45.0g Pancreatic digest of casein .......................................................... 17.0g Agar ............................................................................................ 12.0g Papaic digest of soybean meal ...................................................... 3.0g Glucose ......................................................................................... 2.5g K2HPO4 ......................................................................................... 2.5g pH 7.0 ± 0.2 at 25°C

Use: For the isolation and cultivation of Brevibacterium linens.

Tryptone Soy Agar with Sheep Blood (LMG Medium 175) Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein .......................................................... 15.0g NaCl .............................................................................................. 5.0g Pancreatic digest of soybean meal ................................................ 5.0g Sheep blood, defibrinated ........................................................50.0mL pH 7.3 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

Tryptone Soy Broth, HiVeg (Antibiotic Assay No. 37, HiVeg) Composition per liter: Plant hydrolysate ........................................................................ 15.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 5.0g pH 7.3 ± 0.1 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 10 min at 15 psi pressure–121°C.

Use: A general purpose medium for cultivating a wide variety of fastidious microrganisms.

Tryptone Soy Broth with 0.1% Tween™ 80 Composition per liter: Pancreatic digest of casein.......................................................... 15.0g Papaic digest of soybean meal...................................................... 5.0g NaCl.............................................................................................. 5.0g Tween™ 80................................................................................... 1.0g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Corynebacterium genitalium and Corynebacterium pseudogenitalium.

Tryptone Soy Broth with Yeast Extract Composition per liter: Pancreatic digest of casein.......................................................... 17.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 3.0g Pancreatic digest of soybean meal................................................ 3.0g

Tryptone Soy HiVeg Broth with 10% Sodium Chloride and 1% Sodium Pyruvate

Preparation of Medium: Add components to distilled/deionized

1843

Plant peptone ................................................................................ 5.0g NaCl.............................................................................................. 5.0g MgSO4 .......................................................................................... 1.5g pH 7.3 ± 0.2 at 25°C

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Source: This medium is available as a premixed powder from Hi-

Use: For the cultivation of Carnobacterium piscicola.

Tryptone Soy HiVeg Agar with Added Sodium Chloride and Cocarboxylase Composition per liter: Agar ............................................................................................ 15.0g Plant hydrolysate......................................................................... 15.0g NaCl ............................................................................................ 10.0g Papaic digest of soybean meal ...................................................... 5.0g Cocarboxylase solution..........................................................100.0mL pH 7.3 ± 0.2 at 25°C

Source: This medium, without cocarboxylase, is available as a premixed powder from HiMedia.

Cocarboxylase Solution: Composition per 100.0mL: Cocarboxylase............................................................................ 2.0mg

Preparation of Cocarboxylase Solution: Add cocarboxylase to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Use: For the cultivation and maintenance of Haemophilus piscium.

Tryptone Soy HiVeg Agar with Lecithin and Polysorbate 80 Composition per liter:

Media.

Use: For the cultivation of Escherichia coli for bacteriophage production.

Tryptone Soy HiVeg Broth with 0.1% Agar (Soybean HiVeg Medium with 0.1% Agar) Composition per liter: Plant hydrolysate ........................................................................ 17.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g Glucose ......................................................................................... 2.5g K2HPO4......................................................................................... 2.5g Agar .............................................................................................. 1.0g pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Use: For the cultivation of anaerobes from root canals, blood, and other clinical specimens and for determining glucose fermentation.

Tryptone SoyHiVeg Broth without Glucose (Soybean HiVeg Medium) Composition per liter:

Agar ............................................................................................ 15.0g Plant hydrolysate......................................................................... 15.0g Papaic digest of soybean meal ...................................................... 5.0g NaCl .............................................................................................. 5.0g Lecithin ......................................................................................... 0.7g Polysorbate 80............................................................................5.0mL pH 7.3 ± 0.2 at 25°C

Plant hydrolysate ........................................................................ 17.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g pH 7.3 ± 0.2 at 25°C

Source: This medium, without polysorbate 80, is available as a pre-

Preparation of Medium: Add components to distilled/deionized

mixed powder from HiMedia.

Source: This medium is available as a premixed powder from HiMedia. water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of a wide variety of microorganisms. For sterility testing.

Use: For determining the efficiency of sanitization of containers, surfaces, and water-miscible cosmetics.

Tryptone Soy HiVeg Agar with Magnesium Sulfate (TSAM HiVeg) Composition per liter: Agar ............................................................................................ 15.0g Plant hydrolysate......................................................................... 15.0g © 2010 by Taylor and Francis Group, LLC

Tryptone Soy HiVeg Broth with 10% Sodium Chloride and 1% Sodium Pyruvate Composition per liter: NaCl.......................................................................................... 105.0g Plant hydrolysate ........................................................................ 17.0g Sodium pyruvate......................................................................... 10.0g Papaic digest of soybean meal...................................................... 3.0g

1844

Tryptone Soy Salt HiVeg Agar with Magnesium Sulfate

Glucose ......................................................................................... 2.5g K2HPO4 ......................................................................................... 2.5g pH 7.3 ± 0.2 at 25°C

Glucose ......................................................................................... 2.5g K2HPO4......................................................................................... 2.5g pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-

Media.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat until dissolved. Adjust pH to 7.3. Distribute into tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of Staphylococcus aureus from foods.

Use: For the cultivation and maintenance of a wide variety of het-

Tryptone Soy Salt HiVeg Agar with Magnesium Sulfate Composition per liter: Plant hydrolysate......................................................................... 50.0g NaCl ............................................................................................ 30.0g Agar ............................................................................................ 15.0g Papaic digest of soybean meal ...................................................... 5.0g MgSO4 .......................................................................................... 1.5g pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Tryptone Soy Yeast Extract HiVeg Agar Composition per liter: Plant hydrolysate......................................................................... 17.0g Agar ............................................................................................ 15.0g Yeast extract.................................................................................. 6.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g Glucose ......................................................................................... 2.5g K2HPO4 ......................................................................................... 2.5g pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Use: For the cultivation and maintenance of a wide variety of heterotrophic microorganisms. For the confirmation of Listeria in Henry's light.

Tryptone Soy Yeast Extract HiVeg Broth Composition per liter: Plant hydrolysate......................................................................... 17.0g Yeast extract.................................................................................. 6.0g NaCl .............................................................................................. 5.0g Papaic digest of soyabean meal .................................................... 3.0g © 2010 by Taylor and Francis Group, LLC

erotrophic microorganisms.For the confirmation of Listeria in Henry's light.

Tryptone Thioglycolate Medium (DSMZ Medium 48) Composition per liter: Yeast extract.................................................................................. 6.0g K2HPO4....................................................................................... 5.45g Peptone ......................................................................................... 2.0g Tryptone........................................................................................ 2.0g KH2PO4......................................................................................... 1.2g Na-thioglycolate ........................................................................... 0.5g MgSO4·7H2O ............................................................................ 0.025g CaCl2·2H2O .............................................................................. 0.015g FeSO4·7H2O................................................................................ 0.01g CoCl2·6H2O ............................................................................... 2.5mg Na2MoO4·2H2O ......................................................................... 2.5mg MnCl2·4H2O .............................................................................. 2.0mg Glucose solution ......................................................................50.0mL pH 7.5 ± 0.2 at 25°C

Glucose Solution: Composition per 50.0mL: D-Glucose .................................................................................... 20.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except glucose solution, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 50.0mL of sterile glucose solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Clostridium beijerinckii.

Tryptone Thioglycolate Medium Composition per liter: Yeast extract.................................................................................. 6.0g K2HPO4....................................................................................... 5.45g Peptone ......................................................................................... 2.0g Tryptone........................................................................................ 2.0g KH2PO4....................................................................................... 1.25g Sodium thioglycolate .................................................................... 0.5g MgSO4·7H2O ............................................................................ 0.025g CaCl2·2H2O .............................................................................. 0.015g FeSO4·7H2O................................................................................ 0.01g CoCl2·6H2O ............................................................................... 2.5mg Na2MoO4·2H2O ........................................................................ 2.5mg

Tryptone Yeast Extract Agar 1

MnCl2·4H2O............................................................................... 2.0mg Glucose solution ......................................................................50.0mL pH 7.5 ± 0.2 at 25°C

Glucose Solution: Composition per 50.0mL: Glucose ....................................................................................... 20.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.

Use: For the cultivation and maintenance of Clostridium butyricum and Clostridium roseum.

Tryptone Water Broth (Tryptone Broth) Composition per liter: Pancreatic digest of casein .......................................................... 10.0g NaCl .............................................................................................. 5.0g pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath.

Preparation of Medium: Dissolve 15.0g in 1.0L of distilled water and distribute into final containers. Sterilize by autoclaving at 121°C for 15 min. Use: For the cultivation of production of indole by microorganisms.

Tryptone Water, HiVeg (Tryptone Broth, HiVeg) Composition per liter: Plant hydrolysate......................................................................... 20.0g NaCl .............................................................................................. 5.0g pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Tryptone Water Broth, HiVeg Composition per liter: Plant hydrolysate......................................................................... 10.0g Glucose ......................................................................................... 5.0g K2HPO4 ....................................................................................... 1.25g Yeast extract.................................................................................. 1.0g Bromcresol Purple ...................................................................... 0.04g pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

1845

tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation of Salmonella species from foods.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of fastidious aerobic and facultative microorganisms such as Escherichia coli and Pseudomonas species.

Tryptone Yeast Extract Agar Composition per liter: Pancreatic digest of casein.......................................................... 10.0g Agar .............................................................................................. 2.0g Yeast extract.................................................................................. 1.0g Bromcresol Purple ...................................................................... 0.04g Carbohydrate solution............................................................100.0mL pH 7.0 ± 0.2 at 25°C

Carbohydrate Solution: Composition per 100.0mL: Carbohydrate............................................................................... 10.0g

Preparation of Carbohydrate Solution: Add carbohydrate to distilled/deionized water and bring volume to 100.0mL. Glucose or mannitol may be used. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except carbohydrate solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Adjust pH to 7.0. Gently heat and bring to boiling. Distribute into tubes in 13.5mL volumes. Autoclave for 20 min at 10 psi pressure–115°C. Cool to 45°–50°C. Aseptically add 1.5mL of carbohydrate solution to each tube. Mix thoroughly. Solidify agar quickly by placing tubes in ice water. Use: For the cultivation and differentiation of Staphylococcus aureus based on glucose and mannitol fermentation. Bacteria that ferment the added carbohydrate turn the medium yellow.

Tryptone Yeast Extract Agar 1 Composition per liter: Agar ............................................................................................ 20.0g Pancreatic digest of casein.......................................................... 10.0g Yeast extract.................................................................................. 5.0g K2HPO4 ........................................................................................ 4.4g Glucose ......................................................................................... 2.0g NaCl.............................................................................................. 2.0g pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of a wide variety of heterotrophic bacteria.

1846

Tryptone Yeast Extract HiVeg Agar with Carbohydrate

Tryptone Yeast Extract Broth See: ISP Medium 1

MgSO4 .......................................................................................... 0.1g Resazurin ................................................................................... 0.1mg pH 7.2 ± 0.2 at 25°C

Tryptone Yeast Extract Glucose Medium See: TYG Medium

Preparation of Medium: Add components to distilled/deionized

Tryptone Yeast Extract Glucose Salt Medium See: TYGS Medium

Tryptone Yeast Extract HiVeg Agar with Carbohydrate

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Peptostreptococcus heliotrinreducens. Tryptone Yeast Extract Salt Medium See: TYES Medium

Composition per liter:

Tryptone Yeast Extract Salt Medium

Agar ............................................................................................ 12.0g Plant hydrolysate........................................................................... 6.0g Yeast extract powder..................................................................... 3.0g Carbohydrate solution............................................................100.0mL pH 7.0 ± 0.2 at 25°C

Solution A..............................................................................500.0mL Solution B ..............................................................................500.0mL pH 6.8 ± 0.2 at 25°C

Source: This medium, without carbohydrate solution, is available as a premixed powder from HiMedia.

Solution A: Composition per 500.0mL:

Carbohydrate Solution: Composition per 100.0mL:

NaCl.......................................................................................... 125.0g MgCl2·6H2O ............................................................................... 50.0g K2SO4 ........................................................................................... 5.0g CaCl2·6H2O .................................................................................. 0.2g

Carbohydrate............................................................................... 10.0g

Preparation of Carbohydrate Solution: Add carbohydrate to distilled/deionized water and bring volume to 100.0mL. Glucose or mannitol may be used. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except carbohydrate solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Adjust pH to 7.0. Gently heat and bring to boiling. Distribute into tubes in 13.5mL volumes. Autoclave for 20 min at 10 psi pressure–115°C. Cool to 45°–50°C. Aseptically add 1.5mL of carbohydrate solution to each tube. Mix thoroughly. Solidify agar quickly by placing tubes in ice water. Use: For the cultivation and differentiation of Staphylococcus aureus based on glucose and mannitol fermentation. Bacteria that ferment the added carbohydrate turn the medium yellow.

Tryptone Yeast Extract Medium Composition per liter: Pancreatic digest of casein .......................................................... 10.0g Yeast extract.................................................................................. 1.0g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Haloferax volcanii.

Tryptone Yeast Extract Mineral Medium Composition per liter: Pancreatic digest of casein .......................................................... 10.0g Yeast extract................................................................................ 10.0g NaHCO3 ........................................................................................ 6.0g Arginine ........................................................................................ 3.0g NaCl .............................................................................................. 1.0g K2HPO4 ......................................................................................... 0.5g KH2PO4 ......................................................................................... 0.5g L-Cysteine·HCl.............................................................................. 0.3g CaCl2 ............................................................................................. 0.1g © 2010 by Taylor and Francis Group, LLC

Composition per liter:

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 6.8. Autoclave for 15 min at 15 psi pressure–121°C.

Solution B: Composition per 500.0mL: Pancreatic digest of casein............................................................ 5.0g Yeast extract.................................................................................. 5.0g

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 6.8. Autoclave for 15 min at 15 psi pressure–121°C. Preparation of Medium: Aseptically combine 500.0mL of solution A with 500.0mL of solution B. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Haloferax volcanii.

Tryptophan Assay Medium Composition per liter: Glucose ....................................................................................... 40.0g Sodium acetate............................................................................ 20.0g Casamino acids ........................................................................... 12.0g K2HPO4......................................................................................... 1.0g KH2PO4......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.4g L-Cystine ....................................................................................... 0.2g Adenine sulfate ........................................................................... 0.02g FeSO4·7H2O................................................................................ 0.02g Guanine·HCl ............................................................................... 0.02g MnSO4·7H2O .............................................................................. 0.02g NaCl............................................................................................ 0.02g Uracil .......................................................................................... 0.02g Pyridoxine·HCl .......................................................................... 0.4mg Riboflavin .................................................................................. 0.4mg p-Aminobenzoic acid................................................................. 0.2mg Calcium pantothenate ................................................................ 0.2mg Niacin......................................................................................... 0.2mg

Tryptose Agar with Citrate

Thiamine·HCl ............................................................................ 0.2mg Biotin ..........................................................................................0.8μg pH 6.7 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes in 5.0mL volumes. Add standard solutions and test solutions to each tube. Bring volume of each tube to 10.0mL. Autoclave for 15 min at 15 psi pressure–121°C.

1847

Preparation of Medium: Add pancreatic digest of casein to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Use: For the differentiation of bacteria, especially members of the Enterobacteriaceae, based on their production of indole.

Tryptose Agar Composition per liter:

Composition per 100.0mL:

L-Tryptophan ................................................................................. 0.5g

Source: This medium is available as a premixed powder from BD Di-

Use: For the assay of tryptophan using Lactobacillus plantarum as an indicator organism.

Tryptophan Broth NaCl .............................................................................................. 0.5g KH2PO4 ....................................................................................... 0.25g pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 7.4. Filter sterilize. Aseptically distribute in 1.0mL volumes into sterile screw-capped tubes.

agnostic Systems.

Use: For the cultivation and maintenance of fastidious aerobic and facultative microorganisms.

Use: For the cultivation of Flavobacterium species and a variety of other bacteria. Also used to differentiate bacteria based on indole production. Indole is determined by the addition of modified Kovacs reagent to cultures that have incubated for 18–24 hr. Formation of a red color in the upper layer indicates indole formation.

Tryptophan HiVeg Medium Composition per liter: Plant hydrolysate......................................................................... 10.0g NaCl .............................................................................................. 5.0g DL-Tryptophan .............................................................................. 1.0g pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Preparation of Medium: Add components to distilled/deionized

Tryptose Agar (BAM M167) Composition per liter: Tryptose ...................................................................................... 20.0g Agar ............................................................................................ 15.0g NaCl.............................................................................................. 5.0g Glucose ......................................................................................... 1.0g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Pour into sterile Petri dishes or leave in tubes. For slants allow tubes to cool in an inclined position.

water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 7.4. Filter sterilize. Aseptically distribute in 1.0mL volumes into sterile screw-capped tubes.

Use: For the cultivation of a variety of bacteria for serology.

Use: For the cultivation of Flavobacterium species and a variety of

Composition per liter:

other bacteria. Also used to differentiate bacteria based on indole production. Indole is determined by the addition of modified Kovacs reagent to cultures that have incubated for 18–24 hr. Formation of a red color in the upper layer indicates indole formation.

Tryptophan 1% Solution (Trypticase™ 1% Solution) (Tryptone 1% Solution) Composition per liter:

Tryptose Agar with Citrate

Preparation of Medium: Add components to distilled/deionized

Pancreatic digest of casein .......................................................... 10.0g pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di-

Use: For the cultivation and maintenance of fastidious aerobic and fac-

agnostic Systems.

ultative microorganisms, including Brucella species and streptococci.

1848

Tryptose Agar, HiVeg

Tryptose Agar, HiVeg Composition per liter: Plant hydrolysate No. 1............................................................... 20.0g Agar ............................................................................................ 15.0g NaCl .............................................................................................. 5.0g Glucose ......................................................................................... 1.0g pH 7.2 ± 0.2 at 25°C

NaCl.............................................................................................. 5.0g Glucose ......................................................................................... 1.0g Thiamine·HCl ............................................................................ 5.0mg pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

Source: This medium is available as a premixed powder from Hi-

Media.

Use: For the cultivation and maintenance of fastidious aerobic and fac-

Preparation of Medium: Add components to distilled/deionized

ultative microorganisms, including Brucella species and streptococci.

Composition per liter:

Use: For the cultivation and maintenance of fastidious aerobic and facultative microorganisms. For the isolation, cultivation, and differentiation of Brucella, sreptococci, and pneumococci.

Tryptose Agar with Sheep Blood (ATCC Medium 546) Composition per liter: Agar ............................................................................................ 15.0g Tryptose ...................................................................................... 10.0g NaCl .............................................................................................. 5.0g Beef extract ................................................................................... 3.0g Sheep blood, defibrinated .............................................50.0–100.0mL pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except sheep blood, to distilled/deionized water and bring volume to 900–950mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile sheep blood. Mix thoroughly. Pour into sterile Petri dishes in 17.0mL volumes or distribute into sterile tubes. Use: For the cultivation and maintenance of a wide variety of fastidious microorganisms.

Tryptose Agar with Thiamine Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein .......................................................... 10.0g Peptic digest of animal tissue...................................................... 10.0g NaCl .............................................................................................. 5.0g Glucose ......................................................................................... 1.0g Thiamine·HCl ............................................................................ 5.0mg pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of fastidious aerobic and facultative microorganisms, including Brucella species and streptococci.

Tryptose Agar with Thiamine HCl, HiVeg Composition per liter: Agar ............................................................................................ 15.0g Plant peptone............................................................................... 10.0g Plant hydrolysate......................................................................... 10.0g © 2010 by Taylor and Francis Group, LLC

Tryptose Blood Agar Agar ............................................................................................ 12.0g Tryptose ...................................................................................... 10.0g NaCl.............................................................................................. 5.0g Beef extract................................................................................... 3.0g Sheep blood, defibrinated ........................................................70.0mL pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath.

Preparation of Medium: Add components, except sheep blood, to distilled/deionized water and bring volume to 930.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile sheep blood. Mix thoroughly. Pour into sterile Petri dishes in 17.0mL volumes or distribute into sterile tubes. Use: For the cultivation and maintenance of a wide variety of fastidious microorganisms.

Tryptose Blood Agar Composition per liter: Agar ............................................................................................ 20.0g Proteose peptone No. 3 ............................................................... 10.0g Tryptose ...................................................................................... 10.0g Beef extract................................................................................... 5.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g Sheep blood ...........................................................................100.0mL L-Cysteine·HCl solution ............................................................2.5mL L-Cysteine·HCl

Solution: Composition per 10.0mL: L-cysteine·HCl

............................................................................. 1.0g

Preparation of L-Cysteine·HCl Solution: Dissolve 1.0g of Lcysteine·HCl in distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Warm to 50°C.

Preparation of Medium: Add components, except sheep blood and L-cysteine·HCl solution, to distilled/deionized water and bring volume to 887.5mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Warm sheep blood to 50°C. Aseptically add 100.0mL of sterile sheep blood and 2.5mL of sterile L-cysteine·HCl solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Corynebacterium matruchotii, Propionibacterium propionicum, and Staphylococcus saccharolyticus.

Tryptose Broth with Citrate

Tryptose Blood Agar Base Composition per liter: Agar ............................................................................................ 15.0g Tryptose ...................................................................................... 10.0g NaCl .............................................................................................. 5.0g Beef extract ................................................................................... 3.0g

1849

NaCl.............................................................................................. 5.0g Plant extract .................................................................................. 3.0g Yeast extract.................................................................................. 1.0g Sheep blood, defibrinated ........................................................70.0mL pH 7.2 ± 0.2 at 25°C

Source: This medium, without blood, is available as a premixed pow-

Preparation of Medium: Add components to distilled/deionized

der from HiMedia.

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to cool in a slanted position to obtain a 4– 5.0cm slant and a 2–3.0cm butt.

Use: For the cultivation and enumeration of Salmonella species from foods.

Tryptose Blood Agar Base, HiVeg with Sheep Blood Composition per liter: Agar ............................................................................................ 15.0g Plant hydrolysate No. 1............................................................... 10.0g NaCl .............................................................................................. 5.0g Plant extract .................................................................................. 3.0g Sheep blood, defibrinated ........................................................70.0mL pH 7.2 ± 0.2 at 25°C

Source: This medium, without blood, is available as a premixed powder from HiMedia.

Tryptose Blood Agar Base with Yeast Extract Composition per liter: Agar ............................................................................................ 15.0g Tryptose ...................................................................................... 10.0g NaCl .............................................................................................. 5.0g Beef extract ................................................................................... 3.0g Yeast extract.................................................................................. 1.0g Sheep blood, defibrinated ........................................................50.0mL pH 7.3 ± 0.2 at 25°C

Use: For the cultivation and maintenance of a wide variety of fastidious microorganisms. For the isolation of fastidious organisms and determining hemolytic reactions.

Tryptose Blood Agar Base 298 Medium See: TBAB 298 Medium

Tryptose Broth Composition per liter: Pancreatic digest of casein.......................................................... 10.0g Peptic digest of animal tissue ..................................................... 10.0g NaCl.............................................................................................. 5.0g Glucose ......................................................................................... 1.0g pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of fastidious aerobic and facultative microorganisms, including streptococci. For the cultivation of fastidious aerobic and facultative microorganisms.

Tryptose Broth (BAM M167) Composition per liter:

Source: This medium is available as a premixed powder from BD Di-

Tryptose ...................................................................................... 20.0g NaCl.............................................................................................. 5.0g Glucose ......................................................................................... 1.0g pH 7.2 ± 0.2 at 25°C

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

Preparation of Medium: Add components, except sheep blood, to

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile sheep blood. Mix thoroughly. Pour into sterile Petri dishes in 17.0mL volumes or distribute into sterile tubes.

Use: For the cultivation and maintenance of a wide variety of fastidious microorganisms.

Tryptose Blood Agar Base with Yeast Extract, HiVeg Composition per liter: Agar ............................................................................................ 15.0g Plant hydrolysate No. 1............................................................... 10.0g © 2010 by Taylor and Francis Group, LLC

Use: For the cultivation of a variety of bacteria for serology.

Tryptose Broth with Citrate Composition per liter: Pancreatic digest of casein.......................................................... 10.0g Peptic digest of animal tissue ..................................................... 10.0g Sodium citrate............................................................................. 10.0g NaCl.............................................................................................. 5.0g Glucose ......................................................................................... 1.0g Thiamine·HCl ............................................................................ 5.0mg pH 7.2 ± 0.2 at 25°C

1850

Tryptose Broth, HiVeg

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of a variety of fastidious aerobic microorganisms, especially Brucella species, from clinical sources and dairy products.

Tryptose Broth, HiVeg Composition per liter: Plant hydrolysate No. 1............................................................... 20.0g NaCl .............................................................................................. 5.0g Glucose ......................................................................................... 1.0g pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Cycloserine Solution: Composition per 10.0mL: D-Cycloserine ................................................................................ 0.4g

Preparation of Cycloserine Solution: Add D-cycloserine to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except cycloserine solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile cycloserine solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of Clostridium species, especially Clostridium botulinum, from foods.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of fastidious aerobic and facultative microorganisms, including streptococci.

Tryptose Cycloserine Glucose HiVeg Agar Base with Cycloserine

Tryptose Phosphate Agar Composition per liter: Tryptose ...................................................................................... 20.0g Agar ............................................................................................ 15.0g NaCl.............................................................................................. 5.0g Na2HPO4 ....................................................................................... 2.5g Glucose ......................................................................................... 2.0g pH 7.3 ± 0.2 at 25°C

Composition per liter:

Preparation of Medium: Add components to distilled/deionized

Agar ............................................................................................ 20.0g Plant hydrolysate No. 1............................................................... 15.0g Papaic digest of soybean meal ...................................................... 5.0g Yeast extract.................................................................................. 5.0g Ferric ammonium citrate............................................................... 1.0g Cycloserine solution ................................................................10.0mL pH 7.6 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Erysipelothris tonsillarum.

Source: This medium, without cycloserine, is available as a premixed powder from HiMedia.

Cycloserine Solution: Composition per 10.0mL: D-Cycloserine ................................................................................ 0.4g

Preparation of Cycloserine Solution: Add D-cycloserine to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Use: For the isolation and cultivation of Clostridium species, especially Clostridium botulinum, from foods.

Tryptose Cycloserine Dextrose Agar Composition per liter: Agar ............................................................................................ 20.0g Tryptose ...................................................................................... 15.0g Pancreatic digest of soybean meal ................................................ 5.0g Yeast extract.................................................................................. 5.0g Ferric ammonium citrate............................................................... 1.0g Cycloserine solution ................................................................10.0mL pH 7.6 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

Tryptose Phosphate Broth Composition per liter: Tryptose ...................................................................................... 20.0g NaCl.............................................................................................. 5.0g Na2HPO4 ....................................................................................... 2.5g Glucose ......................................................................................... 2.0g pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems and Oxoid Unipath.

Use: For the cultivation of a variety of bacteria. For cell culture.

Tryptose Phosphate Broth, HiVeg Composition per liter: Plant hydrolysate No. 1............................................................... 20.0g NaCl.............................................................................................. 5.0g Na2HPO4 ....................................................................................... 2.5g Glucose ......................................................................................... 2.0g pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Tryptose Sulfite Cycloserine Agar with Polymyxin and Kanamycin

inoculation of anaerobic microorganisms, place tubes of sterile medium in a 100°C bath for 15 min and cool undisturbed.

Use: For the cultivation of a variety of fastidious bacteria. For the cultivation of fastidious bacteria and as an adjuvant to tissue culture media.

1851

Ferric ammonium citrate............................................................... 1.0g Na2S2O5 ........................................................................................ 1.0g Egg yolk emulsion ...................................................................50.0mL Cycloserine solution ................................................................10.0mL pH 7.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid

Tryptose Phosphate Broth, Modified Composition per liter: Enzymatic digest of casein ......................................................... 20.0g NaCl .............................................................................................. 5.0g Na2HPO4 ....................................................................................... 2.5g Glucose ......................................................................................... 2.0g pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Prior to the inoculation of anaerobic microorganisms, place tubes of sterile medium in a 100°C bath for 15 min and cool undisturbed.

Use: For the cultivation of a variety of fastidious microorganisms, including pneumococci, streptococci, and meningococci.

Tryptose Sulfite Cycloserine Agar (TSC Agar) Composition per liter: Tryptose ...................................................................................... 15.0g Agar ............................................................................................ 14.0g Pancreatic digest of soybean meal ................................................ 5.0g Yeast extract.................................................................................. 5.0g Ferric ammonium citrate............................................................... 1.0g Na2S2O5 ........................................................................................ 1.0g Cycloserine solution ................................................................10.0mL pH 7.6 ± 0.2 at 25°C

Cycloserine Solution: Composition per 10.0mL: D-Cycloserine ................................................................................ 0.4g

Preparation of Cycloserine Solution: Add cycloserine to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Tryptose Sulfite Cycloserine Agar (TSC Agar) Composition per liter: Tryptose ...................................................................................... 15.0g Agar ............................................................................................ 14.0g Beef extract ................................................................................... 5.0g Pancreatic digest of soybean meal ................................................ 5.0g Yeast extract.................................................................................. 5.0g © 2010 by Taylor and Francis Group, LLC

Unipath.

Egg Yolk Emulsion: Composition: Chicken egg yolks............................................................................ 11 Whole chicken egg ............................................................................ 1

Preparation of Egg Yolk Emulsion: Soak eggs with 1:100 dilution of saturated mercuric chloride solution for 1 min. Crack eggs and separate yolks from whites. Mix egg yolks with 1 chicken egg. Cycloserine Solution: Composition per 10.0mL: D-Cycloserine ................................................................................ 0.4g

Preparation of Cycloserine Solution: Add cycloserine to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except cycloserine solution and egg yolk emulsion, to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile cycloserine solution and egg yolk emulsion. Mix thoroughly. Pour into sterile Petri dishes. Use: For the presumptive identification and enumeration of Clostridium perfringens.

Tryptose Sulfite Cycloserine Agar with Polymyxin and Kanamycin Composition per liter: Tryptose ...................................................................................... 15.0g Agar ............................................................................................ 14.0g Beef extract................................................................................... 5.0g Pancreatic digest of soybean meal................................................ 5.0g Yeast extract.................................................................................. 5.0g Ferric ammonium citrate............................................................... 1.0g Na2S2O5 ........................................................................................ 1.0g Antibiotic solution ...................................................................10.0mL pH 7.6 ± 0.2 at 25°C

Antibiotic Solution: Composition per 10.0mL: D-Cycloserine ................................................................................ 0.4g

Polymyxin B sulfate ................................................................... 0.03g Kanamycin sulfate .................................................................... 0.012g

Preparation of Antibiotic Solution: Add components to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except antibiotic solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile antibiotic solution. Mix thoroughly. Pour into sterile Petri dishes. Use: For the isolation and enumeration of Clostridium perfringens from foods and clinical specimens.

1852

Tryptose Sulfite Cycloserine Agar without Egg Yolk

Tryptose Sulfite Cycloserine Agar without Egg Yolk (TSC Agar without Egg Yolk)

Use: For the cultivation of Spirochaeta caldaria.

TS Soil Extract (Trypticase™ Soy Soil Extract)

Composition per liter: Tryptose ...................................................................................... 15.0g Agar ............................................................................................ 14.0g Beef extract ................................................................................... 5.0g Pancreatic digest of soybean meal ................................................ 5.0g Yeast extract.................................................................................. 5.0g Ferric ammonium citrate............................................................... 1.0g Na2S2O5 ........................................................................................ 1.0g Cycloserine solution ................................................................10.0mL pH 7.6 ± 0.2 at 25°C

Pancreatic digest of casein.......................................................... 17.0g Agar ............................................................................................ 15.0g NaCl.............................................................................................. 5.0g Papaic digest of soybean meal...................................................... 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Soil extract .............................................................................250.0mL

Cycloserine Solution: Composition per 10.0mL:

Soil Extract: Composition per 400.0mL:

D-Cycloserine ................................................................................ 0.4g

African Violet soil..................................................................... 154.0g Na2CO3 ......................................................................................... 0.4g

Preparation of Cycloserine Solution: Add cycloserine to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Composition per liter:

Preparation of Soil Extract: Add components to tap water and bring volume to 400.0mL. Autoclave for 60 min at 15 psi pressure– 121°C. Filter through Whatman filter paper.

Use: For the presumptive identification and enumeration of Clostrid-

Use: For the cultivation and maintenance of Bacillus xerothermo-

ium perfringens.

TS Agar (DSMZ Medium 893) Composition per liter: Agar ............................................................................................ 15.0g Sucrose.......................................................................................... 5.0g Tryptone ........................................................................................ 5.0g Beef extract ................................................................................... 3.0g Glucose ......................................................................................... 1.0g pH 7.0 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Saccharococcus thermophilus.

durans.

TSA 5400 Selective Isolation Medium Composition per liter: Pancreatic digest of casein.......................................................... 15.0g Agar ............................................................................................ 15.0g Papaic digest of soybean meal...................................................... 5.0g NaCl.............................................................................................. 5.0g Bovine blood, citrated............................................................100.0mL Spectinomycin solution ...........................................................10.0mL pH 7.3 ± 0.2 at 25°C

Spectinomycin Solution: Composition per 10.0mL: Spectinomycin .............................................................................. 0.4g

Preparation of Spectinomycin Solution: Add spectinomycin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except bovine blood

TS Medium for Spirochaeta caldaria Composition per liter: Pancreatic digest of casein ............................................................ 2.0g Cellobiose ..................................................................................... 1.0g Maltose.......................................................................................... 1.0g Yeast extract.................................................................................. 1.0g Dithiothreitol............................................................................... 0.15g Resazurin ................................................................................... 1.0mg pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Prepare and dispense medium under 100% N2. Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Continue boiling for 3 min. Cool to room temperature while sparging with 100% N2. Anaerobically distribute into anaerobic tubes. Autoclave for 15 min at 15 psi pressure–121°C. Adjust pH to 7.0 © 2010 by Taylor and Francis Group, LLC

and spectinomycin solution, to distilled/deionized water and bring volume to 890.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile bovine blood and 10.0mL of sterile spectinomycin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation of Treponema hyodysenteriae. TSA Blood Agar See: Trypticase™ Soy Agar with Sheep Blood TSA NaCl See: Trypticase™ Soy Agar with 3% NaCl TSA II™ See: Trypticase™ Soy Agar, Modified

TSY Medium

TSA II™with Sheep Blood and Gentamicin See: Trypticase™ Soy Agar with Sheep Blood and Gentamicin

TSBY Salt Medium Composition per liter: NaCl ............................................................................................ 18.0g Pancreatic digest of casein .......................................................... 17.0g MgCl2·6H2O.................................................................................. 4.0g MgSO4·7H2O .............................................................................. 3.45g Yeast extract.................................................................................. 3.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 2.5g KCl.............................................................................................. 0.34g NH4Cl ......................................................................................... 0.25g CaCl2·2H2O................................................................................. 0.14g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Bacillus subtilis, Carnobacterium alterfunditum, and Carnobacterium funditum. TSBY Salt Medium See: Bacillus mascerans Medium TSC Agar See: Tryptose Sulfite Cycloserine Agar

TSC Agar, Fluorocult (Fluorocult TSC Agar) (Fluorocult Tryptose Sulfite Cycloserine Agar) (Tryptose Sulfite Cycloserine Agar, Fluorocult) Composition per liter: Agar ............................................................................................ 15.0g Tryptose ...................................................................................... 15.0g Peptone from soymeal .................................................................. 5.0g Yeast extract.................................................................................. 5.0g Na2S2O5 ........................................................................................ 1.0g Ammonium ferric citrate .............................................................. 1.0g D-Cycloserine................................................................................ 0.2g 4-Methylumbelliferyl-phosphate disodium salt....................... 50.0mg

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cence in long-wave UV light. Thus a strong suggestion for the presence of C. perfringens can be obtained.

TSC Agar without Egg Yolk See: Tryptose Sulfite Cycloserine Agar without Egg Yolk TSFA See: Tryptic Soy Fast Green Agar TSI Agar See: Triple Sugar Iron Agar

TSN Agar (Trypticase™ Sulfite Neomycin Agar) Composition per liter: Pancreatic digest of casein.......................................................... 15.0g Agar ............................................................................................ 13.5g Yeast extract................................................................................ 10.0g Na2SO3 .......................................................................................... 1.0g Ferric citrate.................................................................................. 0.5g Neomycin sulfate ........................................................................ 0.05g Polymyxin sulfate ....................................................................... 0.02g Buffered thioglycolate solution ...............................................50.0mL pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems. Buffered Thioglycolate Solution: Composition per 50.0mL: Buffer solution .........................................................................35.0mL Sodium thioglycolate solution .................................................15.0mL

Preparation of Buffered Thioglycolate Solution: Combine components. Mix thoroughly. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 45°–50°C. Buffer Solution: Composition per 100.0mL: Na2CO3 ....................................................................................... 28.0g K2HPO4......................................................................................... 5.7g

Preparation of Buffer Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Thioglycolate Solution: Composition per 100.0mL: Sodium thioglycolate .................................................................. 13.3g

Source: This medium is available from Merck.

Preparation of Thioglycolate Solution: Add sodium thioglyco-

Preparation of Medium: Add components to distilled/deionized

late to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Pour into sterile Petri dishes.

Use: For the isolation and enumeration of the vegetative and spore forms of Clostridium perfringens in foodstuffs. The culture medium complies with the recommendations of the International Organization for Standardization (ISO) (1978) and the DIN Norm 10165 for the examination of meat and meat products. It also conforms with the APHA recommendations for the examination of foods (1992). DCycloserine inhibits the accompanying bacterial flora and causes the colonies which develop to remain smaller. 4-Methylumbelliferyl-phosphate (MUP) is a fluorogenic substrate for the alkaline and acid phosphatase. The acid phosphatase is a highly specific indicator for C. perfringens. The acid phosphatase splits the fluorogenic substrate MUP forming 4-methylumbelliferone which can be identified as fluores© 2010 by Taylor and Francis Group, LLC

Preparation of Medium: Add components, except buffered thioglycolate solution, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 12 min at 13 psi pressure–118°C. Do not overheat. Cool to 45°– 50°C. Aseptically add buffered thioglycolate solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation of Clostridium perfringens.

TSY Medium (Trypticase™ Soy Yeast Extract Medium) Agar ............................................................................................ 20.0g Pancreatic digest of casein.......................................................... 17.0g Yeast extract.................................................................................. 5.0g

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TSYES Medium

NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 2.5g pH 7.3 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Escherichia coli.

TSYES Medium (Trypticase™ Soy Yeast Extract Starch Medium) Composition per liter: Pancreatic digest of casein .......................................................... 17.0g Agar ............................................................................................ 15.0g NaCl .............................................................................................. 5.0g Papaic digest of soybean meal ...................................................... 3.0g K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 2.5g Yeast extract.................................................................................. 2.0g Soluble starch................................................................................ 1.0g pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Bacillus species. TT Broth See: Tetrathionate Broth TT Broth, Hajna See: Tetrathionate Broth, Hajna TT Broth, USA See: Tetrathionate Broth, USA TTC Agar See: Tetrazolium Tolerance Agar

TTD Medium (DSMZ Medium 480b) Composition per liter: NaCl (marine salts) ..................................................................... 25.0g Sulfur, powder............................................................................. 10.0g Casitone ........................................................................................ 5.0g NH4Cl ......................................................................................... 0.33g CaCl2·2H2O................................................................................. 0.33g MgCl2·6H2O................................................................................ 0.33g KCl.............................................................................................. 0.33g KH2PO4 ....................................................................................... 0.33g Resazurin ................................................................................... 1.0mg Na2S·9H2O solution .................................................................10.0mL Vitamin solution.......................................................................10.0mL Trace elements solution SL-10 ..................................................1.0mL pH 6.9 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

Trace Elements Solution SL-10: Composition per liter: FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

Vitamin Solution: Composition per liter: Pyridoxine-HCl........................................................................ 10.0mg Thiamine-HCl·2H2O.................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg D-Ca-pantothenate ..................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize. Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O.................................................................................... 0.5g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Sparge with N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Store anaerobically. Preparation of Medium: Prepare and dispense medium under an oxygen-free 80% N2 + 20% CO2 gas mixture. Add components, except vitamin solution and Na2S·9H2O solution, to 980.0mL distilled/deionized water. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Adjust pH to 5.9 with concentrated NaOH. Sterilize medium by heating for 1 hr at 90°C–100°C on 3 subsequent days. Sparge with 80% N2 + 20% CO2. Before use, aseptically and anaerobically add 10.0mL sterile vitamin solution and 10.0mL sterile Na2S·9H2O solution. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or flasks.

Use: For the cultivation of Thermococcus stetteri.

TTYSH Medium Composition per liter: Pancreatic digest of casein.......................................................... 10.0g Tryptose ...................................................................................... 10.0g Yeast extract................................................................................ 10.0g Glucose ......................................................................................... 5.0g NaCl.............................................................................................. 5.0g L-Cysteine·HCl ............................................................................. 1.0g K2HPO4......................................................................................... 0.8g KH2PO4......................................................................................... 0.8g

Tween™ 80A Medium

Ascorbic acid ................................................................................ 0.2g Bovine serum, heat inactivated ................................................50.0mL Hemin solution.........................................................................20.0mL

Hemin Solution: Composition per 50.0mL: Hemin....................................................................................... 50.0mg

Preparation of Hemin Solution: Add 50.0mg of hemin to distilled/deionized water and bring volume 50.0mL. Adjust the pH to 10.5 with 1N NaOH. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except tryptose, hemin solution, and bovine serum, to distilled/deionized water and bring volume to 600.0mL. Mix thoroughly. Add tryptose. Mix thoroughly. Gently heat and bring to boiling. Cool to 25°C. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 50.0mL of sterile, heat-inactivated bovine serum and 20.0mL of sterile hemin solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Crithidia fasciculata.

TweenTM 80A Agar Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein ............................................................ 5.0g Yeast extract.................................................................................. 3.0g MgSO4·7H2O ................................................................................ 2.0g Tween™ 80 solution ................................................................50.0mL pH 7.2 ± 0.2 at 25°C

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Preparation of Solution A: Add components to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.

Solution B: Composition per 100.0mL: Tween™ 80................................................................................. 10.0g

Preparation of Solution B: Add Tween™ 80 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.

Preparation of Medium: Aseptically combine 900.0mL sterile solution A and 100.0mL sterile solution B. Mix thoroughly. Pour into sterile Petri dishes. Use: For the cultivation of unclassified bacterium DSM 13023.

TweenTM 80A Broth Composition per liter: Pancreatic digest of casein............................................................ 5.0g Yeast extract.................................................................................. 3.0g MgSO4·7H2O ................................................................................ 2.0g Tween™ 80 solution ................................................................50.0mL pH 7.2 ± 0.2 at 25°C

Tween™ 80 Solution: Composition per 50.0mL: Tween™ 80................................................................................. 10.0g

Preparation of Tween™ 80 Solution: Add Tween™ 80 to dis-

Tween™ 80 Solution: Composition per 50.0mL:

tilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Tween™ 80 ................................................................................. 10.0g

Preparation of Medium: Add components, except Tween™ 80 so-

Preparation of Tween™ 80 Solution: Add Tween™ 80 to dis-

lution, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure– 121°C. Aseptically add 50.0mL of sterile Tween™ 80 solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

tilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except Tween™ 80 solution, to distilled/deionized water and bring volume to 950.0mL. Gently heat and bring to boiling. Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 50.0mL of sterile Tween™ 80 solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation of Agitococcus lubricus. Tween™ 80 Agar See: Polysorbate 80 Agar

Tween™ 80 Agar (DSMZ Medium 884) Composition per liter: Solution A ..............................................................................900.0mL Solution B ..............................................................................100.0mL

Solution A: Composition per 900.0mL: Agar ............................................................................................ 15.0g Peptone........................................................................................ 10.0g NaCl .............................................................................................. 5.0g CaCl2·2H2O................................................................................... 0.1g pH 7.1 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

Use: For the cultivation of Agitococcus lubricus.

Tween™ 80A Medium (DSMZ Medium 618) Composition per liter: Casitone ........................................................................................ 5.0g Yeast extract.................................................................................. 3.0g MgSO4·7H2O ................................................................................ 2.0g Tween™ 80 solution ................................................................50.0mL pH 7.2 ± 0.2 at 25°C

Tween™ 80 Solution: Composition per 50.0mL: Tween™ 80................................................................................. 10.0g

Preparation of Tween™ 80 Solution: Add Tween™ 80 to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except Tween™ 80 solution, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure– 121°C. Aseptically add 50.0mL of sterile Tween™ 80 solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Agitococcus lubricus.

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Tween™ 80 Hydrolysis Broth

Tween™ 80 Hydrolysis Broth Composition per liter: Na2HPO4 ..................................................................................... 5.79g NaH2PO4 ..................................................................................... 3.53g Neutral Red ................................................................................. 0.02g Tween™ 80 solution ..................................................................5.0mL pH 7.0 ± 0.2 at 25°C

Tween™ 80 Solution: Composition per 50.0mL: Tween™ 80 ................................................................................. 10.0g

Preparation of Tween™ 80 Solution: Add Tween™ 80 to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except Tween™ 80 solution, to distilled/deionized water and bring volume to 995.0mL. Mix thoroughly. Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure– 121°C. Aseptically add 5.0mL of sterile Tween™ 80 solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the differentiation of Mycobacterium species. Strains that hydrolyze Tween™ 80 within 5 days turn the medium pink to red.

Tween™ 80 Hydrolysis Broth Composition per 125.0mL: Neutral Red ................................................................................... 0.1g Solution 1 .................................................................................38.9mL Solution 2 .................................................................................61.1mL Tween™ 80 solution ................................................................25.0mL pH 7.0 ± 0.2 at 25°C

Solution 1: Composition per 400.0mL: KH2PO4 ....................................................................................... 22.7g

Preparation of Solution 1: Add KH2PO4 to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly. Solution 2: Composition per 400.0mL: Na2HPO4 ..................................................................................... 23.8g

Preparation of Solution 2: Add Na2HPO4 to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly. Tween™ 80 Solution: Composition per 50.0mL:

Neutral Red (0.1% solution) ......................................................2.0mL Tween™ 80................................................................................0.5mL pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Combine components. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the differentiation of Mycobacterium species. Strains that hydrolyze Tween™ 80 within 5 days turn the medium pink to red.

Tween™ 80 Hydrolysis Medium Composition per liter: Agar ............................................................................................ 12.0g Peptone ....................................................................................... 10.0g Tween™ 80................................................................................. 10.0g NaCl.............................................................................................. 5.0g CaCl2 ............................................................................................. 0.1g pH 7.2–7.4 at 25°C

Use: For the cultivation and differentiation of Pseudomonas species based on their ability to hydrolyze Tween™ 80. Bacteria that hydrolyze Tween™ 80 appear as colonies surrounded by an opaque zone.

Tween™ 80 Oxgall Caffeic Acid Agar See: TOC Agar

TY Medium Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein............................................................ 5.0g Yeast extract.................................................................................. 3.0g CaCl2·6H2O .................................................................................. 1.3g

Use: For the cultivation of a wide variety of bacteria.

TY Medium (DSMZ Medium 1143)

Tween™ 80 ................................................................................. 10.0g

Composition per liter:

Preparation of Tween™ 80 Solution: Add Tween™ 80 to dis-

Tryptone ....................................................................................... 5.0g Yeast extract ................................................................................. 3.0g CaCl2·2H2O .................................................................................. 0.9g pH 6.8 ± 0.2 at 25°C

tilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except Tween™ 80 solution, to distilled/deionized water and bring volume to 975.0mL. Mix thoroughly. Adjust pH to 7.2. Autoclave for 15 min at 15 psi pressure– 121°C. Aseptically add 25.0mL of sterile Tween™ 80 solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the differentiation of Mycobacterium species. Strains that hydrolyze Tween™ 80 within 5 days turn the medium pink to red.

Tween™ 80 Hydrolysis Broth Composition per 102.5mL: NaHPO4 (0.066M solution)......................................................61.1mL KH2PO4 (0.066M solution)......................................................38.9mL © 2010 by Taylor and Francis Group, LLC

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Adjust pH to 6.8.

Use: For the cultivation of Azorhizobium doebereinerae.

TY Medium, 2X Composition per liter: Pancreatic digest of casein.......................................................... 16.0g Yeast extract................................................................................ 10.0g NaCl.............................................................................................. 5.0g pH 7.0 ± 0.2 at 25°C

TYEG Medium

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Preparation of Medium: Add components to distilled/deionized

Use: For the cultivation of Thermomonospora aquaticus, Thermus fil-

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 25 min at 15 psi pressure–121°C.

iformis, Thermus flavus, Thermus ruber, and other Thermus species.

TYE Broth Medium

Use: For the cultivation of Escherichia coli.

TY Salt Medium Composition per liter: NaCl ............................................................................................ 10.0g Pancreatic digest of casein .......................................................... 10.0g Yeast extract.................................................................................. 5.0g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of a wide variety of bacteria.

TY Salts Medium Composition per liter: Pancreatic digest of casein ............................................................ 1.0g Yeast extract.................................................................................. 1.0g Salts solution..........................................................................100.0mL pH 8.2 ± 0.2 at 25°C

Salts Solution: Composition per liter: NaNO3......................................................................................... 6.89g KNO3 .......................................................................................... 1.03g MgSO4·7H2O ................................................................................ 1.0g Nitrilotriacetic acid ....................................................................... 1.0g CaSO4·2H2O ................................................................................. 0.6g NaCl ......................................................................................... 80.0mg FeCl3 solution ..........................................................................10.0mL Trace elements solution ...........................................................10.0mL

Preparation of Salts Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.2 with 1M NaOH. Autoclave for 15 min at 15 psi pressure–121°C.

FeCl3 Solution: Composition per 100.0mL: FeCl3 ........................................................................................ 28.0mg

Preparation of FeCl3 Solution: Add FeCl3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Trace Elements Solution: Composition per liter: MnSO4·H2O .................................................................................. 2.2g H3BO3 ........................................................................................... 0.5g ZnSO4·7H2O ................................................................................. 0.5g CoCl2·6H2O ............................................................................. 46.0mg Na2MoO4·2H2O ....................................................................... 25.0mg CuSO4 ...................................................................................... 16.0mg H2SO4 .........................................................................................0.5mL

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components, except salts solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 100.0mL of sterile salts solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. © 2010 by Taylor and Francis Group, LLC

(ATCC Medium 1972) Composition per liter: Tryptone...................................................................................... 16.0g Yeast extract................................................................................ 10.0g NaCl.............................................................................................. 5.0g Glucose ......................................................................................... 4.0g pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Aeromicrobium erythreum.

TYE-CO See: Clostridium thermoaceticum Medium

TYE HES Medium Composition per 950.0mL: NaCl............................................................................................ 49.7g MgSO4·7H2O .............................................................................. 49.3g Noble agar................................................................................... 10.0g Yeast extract.................................................................................. 0.5g Pancreatic digest of casein............................................................ 0.5g CaCl2·2H2O solution................................................................50.0mL pH 7.2 ± 0.2 at 25°C

CaCl2·2H2O Solution: Composition per 100.0mL: CaCl2·2H2O .................................................................................. 0.3g

Preparation of CaCl2·2H2O Solution: Add the CaCl2·2H2O to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Preparation of Medium: Add components, except CaCl2·2H2O solution, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile CaCl2·2H2O solution. Mix thoroughly. Adjust pH to 7.2. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation of Planococcus species.

TYEG Medium (Trypticase™ Yeast Extract Glucose Medium) Composition per 1050.0mL: NaCl.......................................................................................... 100.0g Pancreatic digest of casein.......................................................... 10.0g Na2HPO4·7H2O............................................................................. 2.1g NH4Cl ........................................................................................... 1.0g KH2PO4......................................................................................... 0.3g MgCl2·6H2O ................................................................................. 0.2g Glucose solution ......................................................................50.0mL Na2S·7H2O solution .................................................................25.0mL Trace minerals solution II ........................................................10.0mL Wolfe’s vitamin solution..........................................................10.0mL Yeast extract solution.................................................................5.0mL

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TYES Medium

Resazurin (0.2% solution)..........................................................1.0mL FeSO4·9H2O (2.5% solution).....................................................25.0μl pH 7.3 ± 0.1 at 25°C

Glucose Solution: Composition per 100.0mL: D-Glucose .................................................................................... 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Aseptically bubble with 90% N2 + 10% CO2 to reduce. Na2S·7H2O Solution: Composition per 100.0mL: Na2S·7H2O .................................................................................... 2.5g

Preparation of Na2S·7H2O Solution: Add Na2S·7H2O to dis-

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Use freshly prepared solution.

Trace Minerals Solution II: Composition per liter: Nitrilotriacetic acid ..................................................................... 12.8g CoCl2·6H2O ................................................................................ 0.17g CaCl2·2H2O................................................................................... 0.1g FeSO4·7H2O................................................................................. 0.1g MnCl2·4H2O.................................................................................. 0.1g NaCl .............................................................................................. 0.1g ZnCl2 ............................................................................................. 0.1g NiSO4·6H2O.............................................................................. 0.026g CuCl2·2H2O ................................................................................ 0.02g Na2SeO3 .................................................................................... 0.017g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O .......................................................................... 0.01g

Preparation of Trace Minerals Solution II: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Filter through Whatman filter paper. Store under N2.

Wolfe’s Vitamin Solution: Composition per liter: Pyridoxine·HCl ........................................................................ 10.0mg Thiamine·HCl ............................................................................ 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg Calcium pantothenate ................................................................ 5.0mg p-Aminobenzoic acid ................................................................. 5.0mg Thioctic acid .............................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid.................................................................................... 2.0mg Cyanocobalamin .....................................................................100.0μg

Preparation of Wolfe’s Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Aseptically bubble with 90% N2 + 10% CO2 to reduce.

Yeast Extract Solution: Composition per 100.0mL: Yeast extract................................................................................ 10.0g

Preparation of Yeast Extract Solution: Add yeast extract to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Aseptically bubble with 90% N2 + 10% CO2 to reduce. Preparation of Medium: Add components—except glucose solution, yeast extract solution, and Wolfe’s vitamin solution—to distilled/de© 2010 by Taylor and Francis Group, LLC

ionized water and bring volume to 960.0mL. Mix thoroughly. Adjust pH to 7.3. Gently heat and bring to boiling under 90% N2 + 10% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically and anaerobically add 50.0mL of sterile glucose solution, 10.0mL of sterile Wolfe’s vitamin solution, and 5.0mL of sterile yeast extract solution. Aseptically and anaerobically distribute into sterile tubes in 5.0mL volumes. Immediately prior to inoculation, aseptically add 0.125mL of sterile Na2S·9H2O solution per tube.

Use: For the cultivation and maintenenace of Halobacteroides acetoethylicus.

TYES Medium (Tryptone Yeast Extract Salt Medium) Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein.......................................................... 10.0g NaCl.............................................................................................. 8.0g Yeast extract.................................................................................. 1.0g CaCl2 ............................................................................................. 0.3g pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenenace of Escherichia coli.

TYG Medium Composition per liter: Yeast extract.................................................................................. 5.0g K2HPO4 ........................................................................................ 3.5g Tryptone........................................................................................ 1.0g L-Cysteinium chloride·H2O .......................................................... 0.5g Na2SO4 .......................................................................................... 0.2g Biotin ....................................................................................... 10.0mg p-Aminobenzoic acid............................................................... 10.0mg Sugar solution ............................................................................5.0mL Resazurin solution .....................................................................4.0mL Trace elements solution .............................................................1.0mL

Resazurin Solution: Composition per 100.0mL: Resazurin ................................................................................. 25.0mg

Preparation of Resazurin Solution: Add resazurin to distilled/ deionized water and bring volume to 100.0mL. Mix thoroughly.

Trace Elements Solution: Composition per 100.0mL: FeCl3·6H2O................................................................................... 2.7g MgSO4 .......................................................................................... 1.2g NaMoO4·2H2O ........................................................................... 0.24g MnSO4·7H2O.............................................................................. 0.17g CaCl2·2H2O ................................................................................ 0.15g ZnSO4·7H2O............................................................................ 29.0mg CuSO4·5H2O............................................................................ 25.0mg CoCl2·6H2O............................................................................. 24.0mg H2SO4 ........................................................................................2.8mL

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

TYGPN Medium

Sugar Solution: Composition per 100.0mL: Glucose ......................................................................................... 1.0g

Preparation of Sugar Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except L-cysteinium chloride, biotin, p-aminobenzoic acid, and sugar solution, to distilled/ deionized water and bring volume to 995.0mL. Mix thoroughly. Adjust pH to 7.0. Gently heat but do not boil. Cook for 5–10 min so that color first turns red and then turns yellow. Cool on ice. Add L-cysteinium chloride, biotin, and p-aminobenzoic acid. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 5.0 mL of sterile sugar solution. Mix thoroughly. Use: For the cultivation of Clostridium species.

TYG Medium Composition per liter:

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Use: For the cultivation and maintenenace of Thermomonospora fusca.

TYGM-9 Medium Composition per liter: NaCl.............................................................................................. 7.5g K2HPO4......................................................................................... 2.8g Casein digest................................................................................. 2.0g Gastric mucin................................................................................ 2.0g Yeast extract.................................................................................. 1.0g KH2PO4......................................................................................... 0.4g Bovine serum, heat inactivated................................................30.0mL Rice starch solution..................................................................30.0mL Tween™ solution.......................................................................0.5mL pH 7.4 ± 0.2 at 25°C

Pancreatic digest of casein .......................................................... 20.0g Glucose ......................................................................................... 5.0g KH2PO4 ......................................................................................... 4.0g Sodium thioglycolate .................................................................... 0.5g Yeast extract.................................................................................. 0.5g MgSO4·7H2O ................................................................................ 0.2g FeSO4·7H2O............................................................................... 5.0mg MnSO4·4H2O ............................................................................. 5.0mg NH4MoO4 .................................................................................. 5.0mg pH 7.4 ± 0.2 at 25°C

Tween™ Solution: Composition per 100.0mL:

Preparation of Medium: Add components to distilled/deionized

Preparation of Rice Starch Solution: Heat sterilize rice starch at

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4 with NaOH. Distribute into tubes or flasks. Autoclave for 10 min at 11 psi pressure–116°C.

Use: For the cultivation of Sprolactobacillus cellulosolvens.

TYG Medium (Trypticase™ Yeast Extract Glucose Medium) (ATCC Medium 603) Composition per liter: Pancreatic digest of casein .......................................................... 10.0g NaCl .............................................................................................. 8.0g Yeast extract.................................................................................. 1.0g Glucose ......................................................................................... 1.0g CaCl2·2H2O................................................................................... 0.3g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenenace of Escherichia coli.

TYG Medium (Tryptone Yeast Extract Glucose Medium) (ATCC Medium 741) Composition per liter: Agar ............................................................................................ 20.0g Pancreatic digest of casein ............................................................ 3.0g Yeast extract.................................................................................. 3.0g Glucose ......................................................................................... 3.0g K2HPO4 ......................................................................................... 1.0g pH 7.4 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

Tween™ 80................................................................................. 10.0g

Preparation of Tween™ Solution: Add Tween™ 80 to absolute ethanol and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Rice Starch Solution: Composition per 100.0mL: Rice starch .................................................................................... 5.0g Phosphate-buffered saline solution........................................100.0mL 150°C for 2 hr. Aseptically add 100.0mL of sterile phosphate-buffered saline solution. Mix thoroughly. Use immediately.

Phosphate-Buffered Saline Solution: Composition per liter: NaCl.............................................................................................. 9.0g Na2HPO4·7H2O......................................................................... 0.795g KH2PO4..................................................................................... 0.114g

Preparation of Phosphate-Buffered Saline Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 25°C.

Preparation of Medium: Add components, except rice starch solution, Tween™ solution, and bovine serum, to distilled/deionized water and bring volume to 939.5mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 30.0mL of sterile bovine serum, 30.0mL of sterile rice starch solution, and 0.5mL of sterile Tween™ solution. Mix thoroughly. Aseptically distribute into sterile, screw-capped tubes or flasks. Use: For the cultivation of Dientamoeba fragilis, Ditrichomonas honigbergii, Entamoeba coli, Entamoeba dispar, Entamoeba gingivalis, Entamoeba insolita, Entamoeba histolytica, Entamoeba moshkovskii, Entamoeba polecki, Entamoeba ranarum, Pseudotrichomonas keilini, and Trepomonas agilis.

TYGPN Medium Composition per liter: Pancreatic digest of casein.......................................................... 20.0g KNO3 .......................................................................................... 10.0g Yeast extract................................................................................ 10.0g

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TYGS Medium

Na2HPO4 ....................................................................................... 5.0g Glycerol (80% solution)...........................................................10.0mL pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 25 min at 15 psi pressure–121°C.

Use: For the cultivation of Escherichia coli.

TYGS Medium (Tryptone Yeast Extract Glucose Salt Medium) Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein .......................................................... 10.0g NaCl .............................................................................................. 8.0g Yeast extract.................................................................................. 1.0g CaCl2·2H2O solution..............................................................100.0mL Glucose solution ....................................................................100.0mL

CaCl2·2H2O Solution: Composition per 100.0mL: CaCl2·2H2O................................................................................... 0.3g

Preparation of CaCl2·2H2O Solution: Add the CaCl2·2H2O to

distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Glucose Solution: Composition per 100.0mL: D-Glucose ...................................................................................... 1.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except CaCl2·2H2O solution and glucose solution, to distilled/deionized water and bring volume to 800.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add the sterile CaCl2·2H2O solution and sterile glucose solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation and maintenance of a variety of bacteria.

Buffer Solution: Composition per 50.0mL: K2HPO4......................................................................................... 1.0g KH2PO4........................................................................................ 0.6g

Preparation of Buffer Solution: Add components to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Adjust pH to 6.8. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Special 107 Vitamin Mix: Composition per 120.0.0mL: Solution 1...................................................................................1.2mL Solution 2...................................................................................0.4mL Solution 3...................................................................................0.4mL Solution 4 vitamins ................................................................100.0mL

Preparation of Special 107 Vitamin Mix: Aseptically combine 1.2mL of sterile solution 1, 0.4mL of sterile solution 2, 0.4mL of sterile solution 3, and 100.0mL of sterile solution 4 vitamins. Bring volume to 120.0mL with sterile distilled/deionized water.

Solution 1: Composition per 100.0mL: Vitamin B12 .............................................................................. 40.0mg

Preparation of Solution 1: Add vitamin B12 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Solution 2: Composition per 100.0.0mL: DL-6,8-Thioctic acid, oxidized............................................... 100.0mg Absolute ethanol ....................................................................100.0mL

Preparation of Solution 2: Add

DL-6,8-thioctic acid to absolute ethanol and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Solution 3: Composition per 100.0mL: Tween™ 80................................................................................. 50.0g Absolute ethanol ....................................................................100.0mL

Preparation of Solution 3: Add Tween™ 80 to absolute ethanol and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Solution 4 Vitamins: Composition per liter:

Glucose ....................................................................................... 10.0g

Vitamin B12 .............................................................................. 10.0mg Calcium D-(+)-pantothenate ...................................................... 2.3mg Choline chloride....................................................................... 1.25mg Riboflavin .................................................................................. 0.7mg Calciferol (vitamin D2) ............................................................ 0.25mg Vitamin A, crystallized alcohol ............................................... 0.25mg p-Aminobenzoic acid............................................................. 0.125mg i-Inositol................................................................................. 0.125mg Biotin ..................................................................................... 0.025mg α-Tocopherol phosphate, disodium salt................................. 0.025mg Folic acid ............................................................................... 0.025mg Menadione (vitamin K3) ........................................................ 0.025mg Thiamine·HCl ........................................................................ 0.025mg Niacin................................................................................... 0.0625mg Niacinamide......................................................................... 0.0625mg Pyridoxal·HCl ...................................................................... 0.0625mg Pyridoxine·HCl .................................................................... 0.0625mg

Preparation of Glucose Solution: Add glucose to distilled/deion-

Preparation of Solution 4 Vitamins: Add components to dis-

ized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

TYI-S-33 Medium Composition per liter: Pancreatic digest of casein ......................................................... 20.0g Yeast extract................................................................................ 10.0g NaCl .............................................................................................. 2.0g L-Cysteine·HCl.............................................................................. 1.0g Ascorbic acid ............................................................................... 0.2g Ferric ammonium citrate.......................................................... 22.8mg Bovine serum, heat inactivated ..............................................100.0mL Special 107 vitamin mix ........................................................100.0mL Buffer solution .........................................................................50.0mL Glucose solution ......................................................................50.0mL pH 6.8 ± 0.2 at 25°C

Glucose Solution: Composition per 50.0mL:

TYM Basal Medium, Modified 3 Preparation of Medium: Add components, except heat-inactivated bovine serum, special 107 vitamin mix, glucose solution, and buffer solution, to distilled/deionized water and bring volume to 700.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 100.0mL of sterile, heat-inactivated bovine serum, 50.0mL of sterile glucose solution, 100.0mL of sterile special 107 vitamin mix, and 50.0mL of sterile buffer solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

1861

Maltose ......................................................................................... 5.0g L-Cysteine·HCl ............................................................................. 1.0g K2HPO4......................................................................................... 0.8g KH2PO4......................................................................................... 0.8g Agar, noble.................................................................................... 0.5g L-Ascorbic acid ............................................................................. 0.2g Horse serum, heat inactivated................................................300.0mL pH 7.2 ± 0.2 at 25°C

Use: For the cultivation of Entamoeba barreti, Entamoeba histolytica, Entamoeba insolita, Entamoeba invadens, Entamoeba moshkovskii, Entamoeba ranarum, Entamoeba terrapinae, Monocercomonas species, Phreatamoeba balamuthi, Spironucleus vortens, and Trichomonas tenax.

Preparation of Medium: Add components, except agar and horse se-

TYM Basal Medium, Modified 1

Use: For the cultivation of Trichomonas gallinae and Tritrichomonas foetus.

Composition per liter: Pancreatic digest of peptone ....................................................... 20.0g Yeast extract................................................................................ 10.0g Maltose.......................................................................................... 5.0g L-Cysteine·HCl.............................................................................. 1.0g K2HPO4 ......................................................................................... 0.8g KH2PO4 ......................................................................................... 0.8g Agar, noble.................................................................................... 0.5g L-Ascorbic acid ............................................................................. 0.2g pH 7.8 ± 0.2 at 25°C

Preparation of Medium: Add components, except agar, to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.8 with NaOH. Add the agar. Mix thoroughly. Gently heat and bring to boiling. Distribute into screw-capped tubes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Trichomonas species.

TYM Basal Medium, Modified 1 Composition per liter: Pancreatic digest of casein .......................................................... 20.0g Yeast extract................................................................................ 10.0g Maltose.......................................................................................... 5.0g L-Cysteine·HCl.............................................................................. 1.0g K2HPO4 ......................................................................................... 0.8g KH2PO4 ......................................................................................... 0.8g Agar, noble.................................................................................... 0.5g L-Ascorbic acid ............................................................................. 0.2g Lamb serum, heat inactivated ................................................300.0mL pH 7.2 ± 0.2 at 25°C

rum, to distilled/deionized water and bring volume to 700.0mL. Mix thoroughly. Adjust pH to 7.2. Add the agar. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 300.0mL of heat-inactivated horse serum. Mix thoroughly. Aseptically distribute into screw-capped tubes.

TYM Basal Medium, Modified 2 Composition per liter: Pancreatic digest of casein.......................................................... 20.0g Yeast extract................................................................................ 10.0g Maltose ......................................................................................... 5.0g L-Cysteine·HCl ............................................................................. 1.0g K2HPO4......................................................................................... 0.8g KH2PO4......................................................................................... 0.8g Agar, noble.................................................................................... 0.5g L-Ascorbic acid ............................................................................. 0.2g Lamb serum, heat inactivated ................................................300.0mL pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components, except agar and lamb serum, to distilled/deionized water and bring volume to 700.0mL. Mix thoroughly. Adjust to pH 7.0 with NaOH. Add the agar. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 300.0mL of sterile, heat-inactivated lamb serum. Mix thoroughly. Aseptically distribute into sterile screw-capped tubes. Use soon after preparation.

Use: For the cultivation of Trichomonas vaginalis and Hypotrichomonas species.

TYM Basal Medium, Modified 3 Composition per liter:

serum, to distilled/deionized water and bring volume to 700.0mL. Mix thoroughly. Adjust to pH 7.2 with NaOH. Add the agar. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 300.0mL of sterile, heat-inactivated lamb serum. Mix thoroughly. Aseptically distribute into sterile screw-capped tubes. Use soon after preparation.

Pancreatic digest of casein.......................................................... 20.0g Yeast extract................................................................................ 10.0g Maltose ......................................................................................... 5.0g L-Cysteine·HCl ............................................................................. 1.0g K2HPO4......................................................................................... 0.8g KH2PO4......................................................................................... 0.8g Agar, noble.................................................................................... 0.5g L-Ascorbic acid ............................................................................. 0.2g Horse serum, heat inactivated................................................300.0mL pH 7.0 ± 0.2 at 25°C

Use: For the cultivation of Hypotrichomonas acosta, Tetratrichomonas

Preparation of Medium: Add components, except agar and horse se-

Preparation of Medium: Add components, except agar and lamb

gallinarum, Trichomitus batrachorum, Trichomonas gallinae, Trichomonas vaginalis, Tritrichomonas augusta, and Tritrichomonas suis.

Composition per liter:

rum, to distilled/deionized water and bring volume to 700.0mL. Mix thoroughly. Adjust pH to 7.0. Add the agar. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 300.0mL of heat-inactivated horse serum. Mix thoroughly. Aseptically distribute into screw-capped tubes.

Pancreatic digest of casein .......................................................... 20.0g Yeast extract................................................................................ 10.0g

Use: For the cultivation of Pentatrichomonas hominis, Proteromonas lacertae, and Tritrichomonas foetus.

TYM Basal Medium, Modified 2

1862

TYM Basal Medium, Modified 3

TYM Basal Medium, Modified 3 Composition per liter: Pancreatic digest of casein .......................................................... 20.0g Yeast extract................................................................................ 10.0g Maltose.......................................................................................... 5.0g L-Cysteine·HCl.............................................................................. 1.0g K2HPO4 ......................................................................................... 0.8g KH2PO4 ......................................................................................... 0.8g Agar, noble.................................................................................... 0.5g L-Ascorbic acid ............................................................................. 0.2g Lamb serum, heat inactivated ................................................200.0mL Dubos medium serum ............................................................100.0mL pH 6.0–6.5 at 25°C

Preparation of Medium: Add components, except agar lamb serum, and Dubos medium serum, to distilled/deionized water and bring volume to 700.0mL. Mix thoroughly. Adjust to pH to 6.0–6.5. Add the agar. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 200.0mL of sterile, heat-inactivated lamb serum and 100.0mL of sterile Dubos medium serum. Mix thoroughly. Aseptically distribute into sterile, screw-capped tubes. Use soon after preparation.

Use: For the cultivation of Pentatrichomonas hominis, Trichomonas vaginalis, Tritrichomonas suis, and Tritrichomonas foetus.

TYM Basal Medium, Modified 4 Composition per liter: Pancreatic digest of casein .......................................................... 20.0g Yeast extract................................................................................ 10.0g Maltose.......................................................................................... 5.0g L-Cysteine·HCl.............................................................................. 1.0g K2HPO4 ......................................................................................... 0.8g KH2PO4 ......................................................................................... 0.8g Agar, noble.................................................................................... 0.5g L-Ascorbic acid ............................................................................. 0.2g Horse serum, heat inactivated ................................................300.0mL pH 6.0 ± 0.2 at 25°C

Preparation of Medium: Add components, except agar and horse

Preparation of Medium: Add components, except NaHCO3 and Lcysteine·HCl·H2O, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Cool to room temperature while sparging with 100% CO2. Add NaHCO3 and Lcysteine·HCl·H2O. Mix thoroughly. Continue sparging with 100% CO2 for 5–10 min. Anaerobically distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Final pH should be 6.7–6.8. Use: For the cultivation of Peptostreptococcus heliotrinreducens.

TYN Medium Composition per liter: Na2S2O3·5H2O ............................................................................ 10.0g Pancreatic digest of casein............................................................ 1.0g Yeast extract.................................................................................. 1.0g Na2SO4 .......................................................................................... 1.0g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Thiobacillus species.

Tyrosine Agar Composition per liter: Solution 1...............................................................................900.0mL Solution 2...............................................................................100.0mL pH 7.0 ± 0.2 at 25°C

Solution 1: Composition per 900.0mL: Agar ............................................................................................ 15.0g Pancreatic digest of gelatin........................................................... 5.0g Beef extract................................................................................... 3.0g

Preparation of Solution 1: Add components to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling.

Solution 2: Composition per 100.0mL: Tyrosine ........................................................................................ 5.0g

serum, to distilled/deionized water and bring volume to 700.0mL. Mix thoroughly. Adjust pH to 6.0. Add the agar. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 50°–55°C. Aseptically add 300.0mL of heat-inactivated horse serum. Mix thoroughly. Aseptically distribute into screwcapped tubes.

Preparation of Solution 2: Add tyrosine to distilled/deionized wa-

Use: For the cultivation of Tritrichomonas foetus and Trichomonas vagi-

Use: For the differentiation of aerobic Actinomycete species. Clearing around a colony indicates utilization of tyrosine. Streptomyces and Actinomadura species utilize tyrosine. Nocardia asteroides, Nocardia caviae, and Mycobacterium fortuitum do not utilize tyrosine.

nalis.

TYM Medium Composition per liter: Pancreatic digest of casein .......................................................... 10.0g Yeast extract................................................................................ 10.0g NaHCO3 ........................................................................................ 6.0g NaCl .............................................................................................. 1.0g K2HPO4 ........................................................................................ 0.5g KH2PO4 ........................................................................................ 0.5g L-Cysteine·HCl·H2O ..................................................................... 0.3g MgSO4 .......................................................................................... 0.1g CaCl2 ............................................................................................. 0.1g pH 6.7–6.8 at 25°C © 2010 by Taylor and Francis Group, LLC

ter and bring volume to 100.0mL. Mix thoroughly. Gently heat and bring to boiling.

Preparation of Medium: Combine solutions 1 and 2. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Tyrosine Agar (International Streptomyces Project Medium 7) (ISP Medium 7) (ATCC Medium 1776) Composition per liter: Agar ............................................................................................ 20.0g Glycerol ...................................................................................... 15.0g L-Tyrosine ..................................................................................... 0.5g L-Asparagine ................................................................................. 1.0g K2HPO4......................................................................................... 0.5g

TZC Selective Medium

MgSO4·7H2O ................................................................................ 0.5g NaCl .............................................................................................. 0.5g FeSO4·7H2O................................................................................ 0.01g Trace elements solution HO-LE ................................................1.0mL pH 7.3 ± 0.1 at 25°C

Trace Elements Solution HO-LE: Composition per liter: H3BO3 ......................................................................................... 2.85g MnCl2·4H2O.................................................................................. 1.8g Sodium tartrate............................................................................ 1.77g FeSO4·7H2O................................................................................ 1.36g CoCl2·6H2O ................................................................................ 0.04g CuCl2·2H2O............................................................................... 0.027g Na2MoO4·2H2O ........................................................................ 0.025g ZnCl2 ........................................................................................... 0.02g

Preparation of Trace Elements Solution HO-LE: Add components

1863

Preparation of Resazurin Solution: Add resazurin to distilled/ deionized water and bring volume to 100.0mL. Mix thoroughly.

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Sugar Solution: Composition per 100.0mL:

Preparation of Medium: Add components to distilled/deionized

Xylose ........................................................................................... 1.0g

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Streptoalloteichus species. For the differentiation of Streptomyces species based on melanine production.

Tyrosine Casein Nitrate Medium (TCN Medium) Composition per liter: Sodium caseinate ........................................................................ 25.0g Agar ............................................................................................ 15.0g NaNO3......................................................................................... 10.0g L-Tyrosine...................................................................................... 1.0g

Use: For the isolation and cultivation of streptomycetes from infected plants.

TYX Medium Composition per liter: Yeast extract.................................................................................. 5.0g K2HPO4 ........................................................................................ 3.5g Tryptone ........................................................................................ 1.0g Na2SO4 .......................................................................................... 0.2g L-cysteinium chloride·H2O ........................................................... 0.5g Biotin ....................................................................................... 10.0mg p-Aminobenzoic acid............................................................... 10.0mg Sugar solution ............................................................................5.0mL Resazurin solution......................................................................4.0mL Trace elements solution .............................................................1.0mL

Preparation of Sugar Solution: Add xylose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except L-cysteinium chloride, biotin, p-aminobenzoic acid, and sugar solution, to distilled/ deionized water and bring volume to 995.0mL. Mix thoroughly. Adjust pH to 7.0. Gently heat but do not boil. Cook for 5–10 min so that color first turns red and then turns yellow. Cool on ice. Add L-cysteinium chloride, biotin, and p-aminobenzoic acid. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 5.0 mL of sterile sugar solution. Mix thoroughly. Use: For the cultivation of Clostridium species.

TZC Selective Medium Composition per liter: Agar ............................................................................................ 17.0g Peptone ......................................................................................... 1.0g Glucose ......................................................................................... 0.5g Pancreatic digest of casein............................................................ 0.1g 2,3,5-Triphenyltetrazolium·HCl solution.................................10.0mL

2,3,5-Triphenyltetrazolium·HCl Solution: Composition per 10.0mL: 2,3,5-Triphenyltetrazolium·HCl ................................................. 0.05g

Preparation of Medium: Add components, except 2,3,5triphenyltetrazolium·HCl solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°– 50°C. Aseptically add 10.0mL of sterile 2,3,5-triphenyltetrazolium·HCl solution. Mix thoroughly. Pour into sterile Petri dishes.

Use: For the isolation, cultivation, and differentiation of Pseudomonas solanacearum. The virulent, wild-type strains appear as irregular to round white colonies with a pink center. Avirulent mutants, which readily occur in nature, appear as round, deep red colonies with a narrow blue border.

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U Agar Plates

U Agar Plates (Ureaplasma Agar Plates) (MES Agar)

U Broth (Ureaplasma Broth) Composition per 99.5mL:

Base agar..................................................................................65.0mL Horse serum .............................................................................20.0mL Yeast dialysate..........................................................................10.0mL MES (2-N-morpholinoethane sulfonic acid) buffer solution...............................................3.0mL Penicillin solution ......................................................................2.0mL Urea solution..............................................................................0.2mL pH 5.5 ± 0.2 at 25°C

Base agar..................................................................................65.0mL Horse serum .............................................................................20.0mL Yeast dialysate .........................................................................10.0mL Penicillin solution ......................................................................2.0mL MES (2-N-morpholinoethane sulfonic acid) buffer solution ..............................................1.0mL Na2SO3 solution.........................................................................1.0mL Urea solution..............................................................................0.5mL pH 5.5 ± 0.2 at 25°C

Base Agar: Composition per liter:

Composition per 100.2mL:

Papaic digest of soybean meal .................................................... 20.0g Agarose ....................................................................................... 10.0g NaCl .............................................................................................. 5.0g Phenol Red (2% solution) ..........................................................1.0mL

Preparation of Base Agar: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.3. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 45°–50°C.

Yeast Dialysate: Composition per 10.0mL: Yeast, active dried ..................................................................... 450.0g

Preparation of Yeast Dialysate: Add active, dried yeast to distilled/deionized water and bring volume to 1250.0mL. Gently heat and bring to 40°C. Autoclave for 15 min at 15 psi pressure–121°C. Put into dialysis tubing. Dialyze against 1.0L of distilled/deionized water for 2 days at 4°C. Discard tubing and its contents. Autoclave dialysate for 15 min at 15 psi pressure–121°C. Store at −20°C.

MES Buffer Solution: Composition per 100.0mL: MES (2-N-morpholinoethane sulfonic acid) buffer ........................................................... 19.52g

Preparation of MES Buffer Solution: Add MES buffer to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 5.5. Filter sterilize.

Penicillin Solution: Composition per 10.0mL: Penicillin .............................................................................. 100,000U

Preparation of Penicillin Solution: Add penicillin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Urea Solution: Composition per 100.0mL: Urea............................................................................................... 6.0g

Preparation of Urea Solution: Add urea to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: To 65.0mL of cooled, sterile base agar, aseptically add 10.0mL of sterile yeast dialysate, 20.0mL of horse serum, 2.0mL of sterile penicillin solution, 3.0mL of sterile MES buffer solution, and 0.2mL of sterile urea solution. Mix thoroughly. Pour into 10mm × 35mm Petri dishes in 5.0mL volumes. Allow plates to stand overnight at 25°C to remove excess surface moisture.

Papaic digest of soybean meal.................................................... 20.0g Agarose ....................................................................................... 10.0g NaCl.............................................................................................. 5.0g Phenol Red (2% solution)..........................................................1.0mL

Yeast Dialysate: Composition per 10.0mL: Yeast, active dried..................................................................... 450.0g

Penicillin Solution: Composition per 10.0mL: Penicillin .............................................................................. 100,000U

Preparation of Penicillin Solution: Add penicillin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

MES Buffer Solution: Composition per 100.0mL: MES (2-N-morpholinoethane sulfonic acid) buffer ........................................................... 19.52g

Preparation of MES Buffer Solution: Add MES buffer to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 5.5. Filter sterilize.

Na2SO3 Solution: Composition per 10.0mL: Na2SO3 ...................................................................................... 0.126g

Preparation of Na2SO3 Solution: Add Na2SO3 to distilled/deion-

ized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Urea Solution: Composition per 100.0mL: Urea............................................................................................... 6.0g

Preparation of Urea Solution: Add urea to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

U9B Broth Preparation of Medium: To 65.0mL of cooled, sterile base agar, aseptically add 10.0mL of sterile yeast dialysate, 20.0mL of horse serum, 2.0mL of sterile penicillin solution, 1.0mL of sterile MES buffer solution, 1.0mL of sterile Na2SO3 solution, and 0.5mL of sterile urea solution. Mix thoroughly. Pour into 10mm × 35mm Petri dishes in 5.0mL volumes. Allow plates to stand overnight at 25°C to remove excess surface moisture. Use within 48 hr. Use: For the isolation and cultivation of Ureaplasma urealyticum.

U9 Broth (Urease Color Test Medium) Composition per 101.6mL: U9 base ....................................................................................95.0mL Horse serum, unheated...............................................................5.0mL Penicillin G solution ..................................................................1.0mL Urea solution..............................................................................0.5mL Phenol Red solution ...................................................................0.1mL pH 6.0 ± 0.2 at 25°C

U9 Base: Composition per 100.0mL: NaCl ............................................................................................ 0.63g Pancreatic digest of casein ........................................................ 0.425g Papaic digest of soybean meal .................................................. 0.075g K2HPO4 ..................................................................................... 0.063g Glucose ..................................................................................... 0.063g KH2PO4 ....................................................................................... 0.02g

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U9 Broth with Amphotericin B Composition per 101.6mL: U9 base ....................................................................................95.0mL Horse serum, unheated...............................................................5.0mL Antibiotic solution .....................................................................1.0mL Urea solution..............................................................................0.5mL Phenol Red solution...................................................................0.1mL pH 6.0 ± 0.2 at 25°C

U9 Base: Composition per 100.0mL: NaCl............................................................................................ 0.63g Pancreatic digest of casein........................................................ 0.425g Papaic digest of soybean meal.................................................. 0.075g K2HPO4..................................................................................... 0.063g Glucose ..................................................................................... 0.063g KH2PO4....................................................................................... 0.02g

Preparation of U9 Base: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 5.5 with 1N HCl. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Antibiotic Solution: Composition per 10.0mL: Penicillin G ................................................................................. 0.63g Amphotericin B ......................................................................... 2.5mg

Preparation of Antibiotic Solution: Add penicillin G and amphotericin B to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Urea Solution: Composition per 30.0mL: Urea............................................................................................... 3.0g

Penicillin G Solution: Composition per 10.0mL:

Preparation of Urea Solution: Add urea to distilled/deionized wa-

Penicillin G ................................................................................. 0.63g

Phenol Red Solution: Composition per 10.0mL:

Preparation of Penicillin G Solution: Add penicillin G to dis-

ter and bring volume to 30.0mL. Mix thoroughly. Filter sterilize.

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Phenol Red.................................................................................... 0.1g

Urea Solution: Composition per 30.0mL:

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Urea............................................................................................... 3.0g

Preparation of Medium: To 95.0mL of cooled, sterile U9 base,

Preparation of Urea Solution: Add urea to distilled/deionized

aseptically add 5.0mL of sterile horse serum, 1.0mL of sterile antibiotic solution, 0.5mL of sterile urea solution, and 0.1mL of sterile Phenol Red solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

water and bring volume to 30.0mL. Mix thoroughly. Filter sterilize.

Phenol Red Solution: Composition per 10.0mL: Phenol Red .................................................................................... 0.1g

Preparation of Phenol Red Solution: Add Phenol Red to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: To 95.0mL of cooled, sterile U9 base, aseptically add 5.0mL of sterile horse serum, 1.0mL of sterile penicillin G solution, 0.5mL of sterile urea solution, and 0.1mL of sterile Phenol Red solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the isolation and identification of T-strain mycoplasmas from clinical specimens, especially Ureaplasma urealyticum. T-mycoplasmas are the only members of the Mycoplasma group known to contain urease. Bacteria with urease activity turn the medium dark pink. © 2010 by Taylor and Francis Group, LLC

Preparation of Phenol Red Solution: Add Phenol Red to dis-

U9B Broth Composition per 102.1mL: U9 base ....................................................................................95.0mL Horse serum, unheated...............................................................5.0mL Penicillin G solution ..................................................................1.0mL Urea solution..............................................................................0.5mL L-Cysteine·HCl·H2O solution ....................................................0.5mL Phenol Red solution...................................................................0.1mL pH 6.0 ± 0.2 at 25°C

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U9C Broth

NaCl............................................................................................ 0.85g Pancreatic digest of casein.......................................................... 0.25g Papaic digest of soybean meal.................................................... 0.15g K2HPO4....................................................................................... 0.12g Glucose ....................................................................................... 0.12g MgCl2·6H2O ................................................................................. 0.2g Yeast extract.................................................................................. 0.1g KH2PO4....................................................................................... 0.02g

Preparation of U9C Base: Add components to distilled/deionized

Penicillin G Solution: Composition per 10.0mL:

Penicillin G ................................................................................ 0.63 g

Preparation of Penicillin G Solution: Add penicillin G to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Urea Solution: Composition per 30.0mL: Urea............................................................................................... 3.0g

Preparation of Urea Solution: Add urea to distilled/deionized water and bring volume to 30.0mL. Mix thoroughly. Filter sterilize. L-Cysteine·HCl·H2O

Solution: Composition per 50.0mL: L-Cysteine·HCl·H2O ..................................................................... 1.0g

water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 5.5 with 2N HCl. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Penicillin G ................................................................................. 0.63g

Preparation of Penicillin G Solution: Add penicillin G to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Urea Solution: Composition per 30.0mL: Urea............................................................................................... 3.0g

Preparation of Urea Solution: Add urea to distilled/deionized water and bring volume to 30.0mL. Mix thoroughly. Filter sterilize. L-Cysteine·HCl·H2O Solution: Composition per 50.0mL: L-Cysteine·HCl·H2O ..................................................................... 1.0g

Preparation of L-Cysteine·HCl·H2O Solution: Add L-cyste-

ine·HCl·H2O to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.

Preparation of L-Cysteine·HCl·H2O Solution: Add L-cysteine·HCl·H2O to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.

Phenol Red Solution: Composition per 10.0mL:

GHL Tripeptide Solution: Composition per 10.0mL:

Phenol Red .................................................................................... 0.1g

Preparation of Phenol Red Solution: Add Phenol Red to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: To 95.0mL of cooled, sterile U9 base, aseptically add 5.0mL of sterile horse serum, 1.0mL of sterile penicillin G solution, 0.5mL of sterile urea solution, 0.5mL of sterile Lcysteine·HCl·H2O solution, and 0.1mL of sterile Phenol Red solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

U9C Broth Composition per 102.0mL: U9C base..................................................................................90.0mL Horse serum, unheated.............................................................10.0mL Penicillin G solution ..................................................................1.0mL Urea solution..............................................................................0.3mL L-Cysteine·HCl·H2O solution ....................................................0.5mL GHL tripeptide solution .............................................................0.1mL Phenol Red solution ...................................................................0.1mL pH 6.0 ± 0.2 at 25°C

GHL tripeptide........................................................................... 0.2mg

Preparation of GHL Tripeptide Solution: Add GHL tripeptide (glycyl-L-histidyl-L-lysine acetate) to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Phenol Red Solution: Composition per 10.0mL: Phenol Red.................................................................................... 0.1g

Preparation of Phenol Red Solution: Add Phenol Red to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: To 90.0mL of cooled, sterile U9C base, aseptically add 10.0mL of sterile horse serum, 1.0mL of sterile penicillin G solution, 0.3mL of sterile urea solution, 0.5mL of sterile Lcysteine·HCl·H2O solution, 0.1mL of sterile GHL tripeptide solution, and 0.1mL of sterile Phenol Red solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

U4 Medium Composition per 100.0mL: Hartley’s digest broth...............................................................20.0mL Fetal calf serum........................................................................15.0mL

Universal Beer HiVeg Agar with Beer

Fresh yeast extract solution......................................................10.0mL Hanks’ balanced salt solution, 10X ...........................................4.0mL MgSO4·5H2O (0.025% solution) ...............................................1.0mL Urea (20% solution).................................................................0.25mL Phenol Red (1% solution) ..........................................................0.2mL pH 6.0–6.2 at 25°C

Hartley’s Digest Broth: Composition per 10.0L:

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FeSO4 ......................................................................................... 6.0mg MnSO4·5H2O ............................................................................. 6.0mg NaCl........................................................................................... 6.0mg Beer........................................................................................250.0mL pH 6.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems and Oxoid Unipath.

Preparation of Medium: Add components, except beer, to distilled/

Ox heart................................................................................... 3000.0g Pancreatin.................................................................................... 50.0g Na2CO3, anhydrous (0.8% solution).............................................5.0L HCl, concentrated ....................................................................80.0mL pH 7.5 ± 0.2 at 25°C

deionized water and bring volume to 750.0mL. Mix thoroughly. Gently heat and bring to boiling. Add beer. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 10 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Preparation of Hartley’s Digest Broth: Finely mince the ox

brewing industry.

heart. Add the meat to 5.0L of distilled/deionized water. Gently heat and bring to 80°C. Add the 5.0L of Na2CO3 solution. Cool to 45°C. Add pancreatin and maintain at 45°C for 4 hr while stirring. Add the HCl and steam at 100°C for 30 min. Cool to room temperature. Adjust pH to 8.0 with 1N NaOH. Gently heat and bring to boiling. Continue boiling for 25 min. Filter while hot. Cool to room temperature. Adjust pH to 7.5. Autoclave for 15 min at 15 psi pressure–121°C.

Fresh Yeast Extract Solution: Composition per 100.0mL: Baker’s yeast, live, pressed, starch-free...................................... 25.0g

Hanks’ Balanced Salt Solution, 10X: Composition per liter: Na2Cl........................................................................................... 80.0g Glucose ....................................................................................... 10.0g KCl................................................................................................ 4.0g CaCl2 ............................................................................................. 1.4g MgCl2·6H2O.................................................................................. 1.0g MgSO4·7H2O ................................................................................ 1.0g Na2HPO4·7H2O............................................................................. 0.9g KH2PO4 ......................................................................................... 0.6g

Preparation of Hanks’ Balanced Salt Solution, 10X: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0–6.2 with HCl. Filter-sterilize medium. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Ureaplasma diversum.

UBA Medium (Universal Beer Agar) Composition per liter: Glucose ....................................................................................... 16.1g Peptonized milk .......................................................................... 15.0g Agar ............................................................................................ 12.0g Tomato juice, dessicated ............................................................. 12.2g Yeast extract.................................................................................. 6.1g K2HPO4 ....................................................................................... 0.31g KH2PO4 ....................................................................................... 0.31g MgSO4·7H2O .............................................................................. 0.12g © 2010 by Taylor and Francis Group, LLC

Use: For the cultivation of microorganisms of significance in the Universal Agar for Yeasts See: Yeast Malt Extract Agar

Universal Beer Agar Composition per liter: Peptonized milk .......................................................................... 15.0g Agar ............................................................................................ 12.0g Yeast extract................................................................................ 10.0g Glucose ....................................................................................... 10.0g Tomato juice solids ....................................................................... 7.0g K2HPO4......................................................................................... 0.5g KH2PO4......................................................................................... 0.5g MgSO4·5H2O ................................................................................ 0.2g NaCl............................................................................................ 0.01g FeSO4·7H2O................................................................................ 0.01g MnSO4·H2O ................................................................................ 0.01g Beer........................................................................................250.0mL pH 6.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium: Add components, except beer, to distilled/deionized water and bring volume to 750.0mL. Mix thoroughly. Gently heat and bring to boiling. Add beer. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 10 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 250.0mL of beer without degassing. Pour into sterile Petri dishes or leave in tubes.

Use: For the enumeration of contaminating bacteria and yeasts encountered in wort and beer. Universal Beer Agar See: UBA Medium

Universal Beer HiVeg Agar with Beer (UB HiVeg Agar) Composition per liter: Glucose ....................................................................................... 16.1g Plant hydrolysate No. 4............................................................... 15.0g Tomato juice ............................................................................... 12.2g Agar ............................................................................................ 12.0g Yeast extract.................................................................................. 6.1g K2HPO4....................................................................................... 0.31g KH2PO4....................................................................................... 0.31g MgSO4 .......................................................................................... 0.12 FeSO4 ......................................................................................... 6.0mg

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Universal Medium for Yeasts

MnSO4 ....................................................................................... 6.0mg NaCl ........................................................................................... 6.0mg Beer ........................................................................................250.0mL pH 6.3 ± 0.2 at 25°C

Source: This medium, without beer, is available as a premixed powder from HiMedia.

Preparation of Medium: Add components, except beer, to distilled/

University of Vermont I Listeria Primary Selective Enrichment Broth See: Listeria Enrichment Broth I, USDA FSIS University of Vermont II Listeria Primary Selective Enrichment Broth See: Listeria Enrichment Broth II, USDA FSIS University of Vermont Modified Listeria Enrichment Broth See: UVM Modified Listeria Enrichment Broth

Use: For cultivation of microorganisms of significance in the brewing industry.

Universal Medium for Yeasts (YM) (DSMZ Medium 186) Composition per liter: Agar ............................................................................................ 15.0g Glucose ....................................................................................... 10.0g Peptone.......................................................................................... 5.0g Yeast extract.................................................................................. 3.0g Malt extract ................................................................................... 3.0g pH 6.8 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Saccharmomyces spp. and other yeasts.

Urea Agar Composition per liter: Agar ............................................................................................ 15.0g Urea............................................................................................. 10.0g Na2HPO4·12H2O .......................................................................... 9.0g NaCl.............................................................................................. 5.0g KH2PO4 ........................................................................................ 1.5g Meat extract .................................................................................. 1.0g Yeast extract.................................................................................. 1.0g MgSO4·7H2O................................................................................ 0.2g MnCl2·4H2O ............................................................................ 20.0mg CaCl2 ......................................................................................... 1.2mg Glucose solution ....................................................................100.0mL

Glucose Solution: Composition per 100.0mL: Glucose ......................................................................................... 5.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Warm to 50°C.

Preparation of Medium: Add components, except glucose solu-

Universal Preenrichment Broth (BAM M188) Composition per liter: KH2PO4 ...................................................................................... 15.0g Na2HPO4....................................................................................... 7.0g Tryptone ........................................................................................ 5.0g Proteose peptone ........................................................................... 5.0g NaCl .............................................................................................. 5.0g Glucose ......................................................................................... 0.5g MgSO4 ........................................................................................ 0.25g Sodium pyruvate ........................................................................... 0.2g Ferric ammonium citrate............................................................... 0.1g pH 6.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until dissolved. Adjust pH to 6.3. Distribute into tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the enrichment of injured foodborne pathogens of different genera simultaneously in lieu of having to undergo separate simultaneous enrichment cultures for subsequent detection or isolation of each pathogen. University of Vermont Listeria Enrichment Broth See: UVM Listeria Enrichment Broth © 2010 by Taylor and Francis Group, LLC

tion, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 100.0mL of sterile glucose solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Corynebacterium glutamicum.

Urea Agar Composition per liter: Agar ............................................................................................ 15.0g Na2HPO4·12H2O .......................................................................... 9.0g NaCl.............................................................................................. 5.0g KH2PO4 ........................................................................................ 1.5g Meat extract .................................................................................. 1.0g Yeast extract.................................................................................. 1.0g MgSO4·7H2O................................................................................ 0.2g MnCl2·4H2O ............................................................................ 20.0mg CaCl2 ......................................................................................... 1.2mg Glucose-urea solution ............................................................100.0mL

Glucose-Urea Solution: Composition per 100.0mL: Urea............................................................................................. 10.0g Glucose ......................................................................................... 5.0g

Urea Broth 10B for Ureaplasma urealyticum Preparation of Glucose-Urea Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Warm to 50°C. Preparation of Medium: Add components, except glucose-urea solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 100.0mL of sterile glucose-urea solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation and maintenance of Corynebacterium glutam-

Preparation of Medium: Add components, except urea solution, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 20 min at 10 psi pressure–115°C. Cool to 50°C. Aseptically add 50.0mL of sterile urea solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Allow tubes to solidify in a slanted position.

icum.

Urea Agar (Urease Test Agar) (Urea Agar Base, Christensen) Composition per liter: Urea............................................................................................. 20.0g Agar ............................................................................................ 15.0g NaCl .............................................................................................. 5.0g KH2PO4 ......................................................................................... 2.0g Peptone.......................................................................................... 1.0g Glucose ......................................................................................... 1.0g Phenol Red ................................................................................ 0.012g pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components, except agar, to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Add agar to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add the 100.0mL of sterile basal medium. Mix thoroughly. Distribute into sterile tubes. Allow tubes to solidify in a slanted position.

Use: For the differentiation of a variety of microorganisms, especially members of the Enterobacteriaceae, aerobic actinomycetes, streptococci, and nonfermenting Gram-negative bacteria, on the basis of urease production.

Urea Agar Base Composition per liter: Agar ............................................................................................ 15.0g NaCl .............................................................................................. 5.0g Na2HPO4 ....................................................................................... 1.2g Peptone.......................................................................................... 1.0g Glucose ......................................................................................... 1.0g KH2PO4 ......................................................................................... 0.8g Phenol Red ................................................................................ 0.012g Urea solution............................................................................50.0mL pH 6.8 ± 0.2 at 25°C

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Urea Agar Base, Christensen See: Urea Agar

Urea Broth Composition per liter: Na2HPO4·12H2O .......................................................................... 9.0g NaCl.............................................................................................. 5.0g KH2PO4 ........................................................................................ 1.5g Meat extract .................................................................................. 1.0g Yeast extract.................................................................................. 1.0g MgSO4·7H2O................................................................................ 0.2g MnCl2·4H2O ............................................................................ 20.0mg CaCl2 ......................................................................................... 1.2mg Glucose-urea solution ............................................................100.0mL

Preparation of Glucose-Urea Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except glucose-urea solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 100.0mL of sterile glucose-urea solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation and maintenance of Corynebacterium glutamicum.

Urea Broth See: Urease Test Broth

Urea Broth 10B for Ureaplasma urealyticum Composition per 100.5mL:

Unipath.

PPLO broth without Crystal Violet..........................................70.0mL Horse serum, unheated.............................................................20.0mL Fresh yeast extract solution .....................................................10.0mL L-Cysteine·HCl·H2O solution.....................................................0.5mL CVA enrichment ........................................................................0.5mL Urea solution..............................................................................0.4mL Phenol Red.................................................................................0.1mL

Urea Solution: Composition per 100.0mL:

PPLO Broth without Crystal Violet: Composition per 900.0mL:

Source: This medium is available as a premixed powder from Oxoid

Urea............................................................................................. 40.0g

Beef heart, solids from infusion.................................................. 16.1g Peptone ....................................................................................... 3.25g NaCl............................................................................................ 1.61g

1870

Urea Broth Base

Preparation of PPLO Broth without Crystal Violet: Add

Source: This medium is available as a premixed powder from Oxoid

components to distilled/deionized water and bring volume to 900.0mL. Adjust pH to 5.5 with 2N HCl. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 37°C.

Unipath.

Fresh Yeast Extract Solution: Composition per 100.0mL:

Urea............................................................................................. 40.0g

Baker’s yeast live, pressed, starch-free....................................... 25.0g

Preparation of Fresh Yeast Extract Solution: Add the live Baker’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90 min at 15 psi pressure–121°C. Allow to stand. Remove supernatant solution. Adjust pH to 6.6–6.8. L-Cysteine·HCl·H2O Solution: Composition per 50.0mL: L-Cysteine·HCl·H2O ..................................................................... 1.0g

Preparation of L-Cysteine·HCl·H2O Solution: Add L-cyste-

ine·HCl·H2O to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.

CVA Enrichment: Composition per liter: Glucose ..................................................................................... 100.0g L-Cysteine·HCl·H2O.................................................................... 25.9g L-Glutamine................................................................................. 10.0g Adenine ......................................................................................... 1.0g L-Cystine·2HCl.............................................................................. 1.0g Nicotinamide adenine dinucleotide ............................................ 0.25g Cocarboxylase............................................................................... 0.1g Guanine·HCl ............................................................................... 0.03g Fe(NO3)3 ..................................................................................... 0.02g Vitamin B12 ................................................................................. 0.01g p-Aminobenzoic acid ................................................................ 0.013g Thiamine·HCl ............................................................................ 3.0mg

Preparation of CVA Enrichment: Add components to distilled/

Urea Solution: Composition per 100.0mL: Preparation of Urea Solution: Add urea to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except urea solution, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Autoclave for 20 min at 10 psi pressure–115°C. Cool to 50°C. Aseptically add 50.0mL of sterile urea solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the differentiation of members of the Enterobacteriaceae based on urease production. Urease-positive bacteria turn the medium pink.

Urea HiVeg Agar Base Autoclavable with Urea (Christensen HiVeg Agar Autoclavable) Composition per liter: Agar ............................................................................................ 15.0g NaCl.............................................................................................. 5.0g Na2HPO4 ....................................................................................... 1.2g Glucose ......................................................................................... 1.0g Plant peptone ................................................................................ 1.0g KH2PO4......................................................................................... 0.8g Phenol Red................................................................................ 0.012g Urea solution............................................................................50.0mL pH 6.8 ± 0.2 at 25°C

Source: This medium, without urea solution, is available as a premixed powder from HiMedia.

deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Urea Solution: Composition per 100.0mL:

Urea Solution: Composition per 30.0mL:

Preparation of Urea Solution: Add urea to distilled/deionized wa-

Urea............................................................................................. 40.0g

Urea............................................................................................... 3.0g

ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Urea Solution: Add urea to distilled/deionized water and bring volume to 30.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except urea solution, to

Preparation of Medium: Aseptically combine the components, except the PPLO broth without Crystal Violet. Aseptically add this mixture to the cooled, sterile PPLO broth without Crystal Violet. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation and maintenance of Ureaplasma urealyticum and other Ureaplasma species. Urease-positive bacteria turn the medium peach orange.

Urea Broth Base Composition per liter: NaCl .............................................................................................. 5.0g Na2HPO4 ....................................................................................... 1.2g Peptone.......................................................................................... 1.0g Glucose ......................................................................................... 1.0g KH2PO4 ......................................................................................... 0.8g Phenol Red ................................................................................ 0.012g Urea solution............................................................................50.0mL pH 6.8 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 20 min at 10 psi pressure–115°C. Cool to 50°C. Aseptically add 50.0mL of sterile urea solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Allow tubes to solidify in a slanted position.

Use: For the detection of Proteus species based on rapid urease activity and the identification of other members of the Enterobacteriaceae based on urease activity. Urease-positive bacteria turn the medium pink. For the detection of urease production, particularly by Proteus vulgaris, micrococci and paracolon organisms.

Urea Nutrient Agar Composition per 1050.0mL: Agar ............................................................................................ 15.0g Peptone ......................................................................................... 5.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 2.0g Beef extract................................................................................... 1.0g Urea solution............................................................................50.0mL

Urea Test Broth

1871

Urea Solution: Composition per 100.0mL:

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.

Urea............................................................................................. 20.0g

Preparation of Medium: Aseptically combine 400.0mL of sterile solution A and 50.0mL of sterile solution B. Mix thoroughly. Aseptically distribute into sterile screw-capped tubes in 7.0mL volumes. Pass the tubes into an anaerobic chamber containing 85% N2 + 10% H2 + 5% CO2 for 60 min. Close screw caps tightly.

Preparation of Urea Solution: Add urea to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except urea solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 50.0mL of sterile urea solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation of Bacillus pantothenticus.

Urea R Broth (Urea Rapid Broth) Composition per liter: Urea............................................................................................. 20.0g Yeast extract.................................................................................. 0.1g Na2HPO4 ................................................................................... 0.095g KH2PO4 ..................................................................................... 0.091g Phenol Red .................................................................................. 0.01g pH 6.9 ± 0.2 at 25°C

Use: For the cultivation and differentiation of anaerobic bacteria based on their production of urease. Bacteria that produce urease turn the medium bright red.

Urea Test Broth Composition per liter: Urea............................................................................................. 20.0g Na2HPO4 ....................................................................................... 9.5g KH2PO4......................................................................................... 9.1g Yeast extract.................................................................................. 0.1g Phenol Red.................................................................................. 0.01g Urea solution..........................................................................100.0mL

Urea Solution: Composition per 100.0mL: Urea............................................................................................. 20.0g

Preparation of Urea Solution: Add urea to distilled/deionized wa-

Source: This medium is available as a prepared medium from BD Di-

ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

agnostic Systems.

Preparation of Medium: Add components, except urea solution, to

Preparation of Medium: Add components to distilled/deionized

distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile urea solution. Mix thoroughly. Aseptically distribute into sterile tubes in 3.0mL volumes.

water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Aseptically distribute into sterile tubes or flasks.

Use: For the differentiation of members of the Enterobacteriaceae based on the rapid detection of urease activity. Urease-positive bacteria turn the medium cerise.

Urea Semisolid Medium Composition per 450.0: Solution A ..............................................................................400.0mL Solution B ................................................................................50.0mL

Solution A: Composition per 400.0mL: Pancreatic digest of casein ............................................................ 6.0g Yeast extract.................................................................................. 2.0g NaCl .............................................................................................. 1.0g Yeast extract.................................................................................. 0.8g Agar .............................................................................................. 0.3g L-Cystine ....................................................................................... 0.1g Thioglycolic acid .....................................................................0.12mL pH 7.2 ± 0.2 at 25°C

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 400.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 60°C.

Solution B: Composition per 50.0mL: Urea............................................................................................... 8.0g Na2HPO4 ....................................................................................... 3.8g KH2PO4 ....................................................................................... 3.64g Yeast extract................................................................................ 0.04g Phenol Red ................................................................................. 4.0mg © 2010 by Taylor and Francis Group, LLC

Use: For the cultivation and differentiation of members of the Enterobacteriaceae and aerobic actinomycetes based on their production of urease. Bacteria that produce urease turn the medium bright red.

Urea Test Broth Composition per 99.6mL: H broth base.............................................................................85.0mL Horse serum .............................................................................10.0mL Penicillin solution ......................................................................2.0mL MES (2-N-morpholinoethane sulfonic acid) buffer solution.....1.0mL Na2SO3 solution.........................................................................1.0mL Urea solution..............................................................................0.5mL Phenol Red solution...................................................................0.1mL pH 7.2 ± 0.2 at 25°C

H Broth Base: Composition per liter: NaCl.............................................................................................. 5.0g Pancreatic digest of casein............................................................ 5.0g Peptone ......................................................................................... 5.0g Beef extract................................................................................... 3.0g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 1.0g

Preparation of H Broth Base: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 4.0mL volumes. Autoclave for 15 min at 10 psi pressure–115°C. Cool to 45°–50°C. Penicillin Solution: Composition per 10.0mL: Penicillin .............................................................................. 100,000U

1872

Ureaplasma Medium

Preparation of Penicillin Solution: Add penicillin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

MES Buffer Solution: Composition per 100.0mL: MES (2-N-morpholinoethane sulfonic acid) buffer.................. 19.52g

Solution B: Composition per 305.0mL: Horse serum ..........................................................................200.0mL Yeastolate solution .................................................................100.0mL Isovitalex....................................................................................5.0mL

Preparation of MES Buffer Solution: Add MES buffer to dis-

Yeastolate Solution: Composition per 100.0mL:

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH to 5.5. Filter sterilize.

Yeastolate.................................................................................... 20.0g

Preparation of Yeastolate Solution: Add yeastolate to distilled/

Na2SO3 Solution: Composition per 10.0mL:

deionized water and bring volume to 100.0mL. Mix thoroughly.

Preparation of Solution B: Combine components. Filter sterilize.

Na2SO3 ...................................................................................... 0.126g

Preparation of Medium: Aseptically add 305.0mL solution B to

Preparation of Na2SO3 Solution: Add Na2SO3 to distilled/deion-

ized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

700.0mL solution A. Mix thoroughly.

Use: For the cultivation of Ureaplasma spp. Urease Color Test Medium See: U9 Broth

Urea Solution: Composition per 100.0mL: Urea............................................................................................... 6.0g

Urease Indole Test Broth See: F35M Hajna Broth

Preparation of Urea Solution: Add urea to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Phenol Red Solution: Composition per 10.0mL:

Urease Test Agar See: Urea Agar

Phenol Red .................................................................................... 0.1g

Urease Test Broth (Urea Broth)

Preparation of Phenol Red Solution: Add Phenol Red to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Composition per liter:

Use: For the cultivation and differentiation of Ureaplasma species

Urea............................................................................................. 20.0g Na2HPO4 ....................................................................................... 9.5g KH2PO4......................................................................................... 9.1g Yeast extract.................................................................................. 0.1g Phenol Red.................................................................................. 0.01g pH 6.8 ± 0.2 at 25°C

based on their production of urease.

Source: This medium is available as a premixed powder from BD Di-

Preparation of Medium: To 85.0mL of cooled sterile H broth base, aseptically add 10.0mL of sterile horse serum, 2.0mL of sterile penicillin solution, 1.0mL of MES buffer solution, 1.0mL of Na2SO3 solution, 0.5mL of urea solution, and 0.1 mL of sterile Phenol Red solution. Mix thoroughly. Aseptically distribute into test tubes in 3.0mL volumes.

agnostic Systems.

Ureaplasma Medium (DSMZ Medium 1096)

Preparation of Medium: Add components to distilled/deionized

Composition per 1005.0mL:

water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Aseptically distribute into sterile tubes or flasks.

Solution A ..............................................................................700.0mL Solution B ..............................................................................305.0mL pH 6.0 ± 0.2 at 25°C

Use: For the differentiation of organisms, especially the Enterobacteriaceae, on the basis of urease production. Urease-positive bacteria turn the medium pink.

Solution A: Composition per 700.0mL: Pancreatic digest of casein ............................................................ 7.0g NaCl .............................................................................................. 5.0g Beef extract ................................................................................... 3.0g Yeast extract.................................................................................. 3.0g Beef heart, solids from infusion.................................................... 2.0g Urea .............................................................................................. 0.4g L-Cysteine-HCl·2H2O ................................................................... 0.1g DNA, fish sperm .......................................................................... 0.2g Phenol Red ................................................................................. 0.02g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 700.0mL. Mix thoroughly. Adjust pH to 6.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. © 2010 by Taylor and Francis Group, LLC

Uric Acid Agar Composition per liter: Agar ............................................................................................ 20.0g Uric acid...................................................................................... 10.0g KH2PO4......................................................................................... 0.5g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Swirl flask while pouring medium.

Use: For the cultivation and maintenance of microorganisms, such as Bacillus fastidiosus, that can utilize uric acid as the sole source of carbon, nitrogen, and energy.

Uric Acid Medium

Uric Acid Agar Composition per liter: Agar ............................................................................................ 15.0g Na2HPO4·12H2O .......................................................................... 9.0g NaCl .............................................................................................. 5.0g KH2PO4 ........................................................................................ 1.5g Meat extract .................................................................................. 1.0g Yeast extract.................................................................................. 1.0g MgSO4·7H2O................................................................................ 0.2g MnCl2·4H2O ............................................................................ 20.0mg CaCl2 .......................................................................................... 1.2mg Glucose-uric acid solution .....................................................100.0mL

Glucose-Uric Acid Solution: Composition per 100.0mL: Glucose ......................................................................................... 5.0g Uric acid........................................................................................ 0.4g

Preparation of Glucose-Uric Acid Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Warm to 50°C.

1873

Preparation of Glucose-Uric Acid Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except glucose-uric acid solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 50°–55°C. Aseptically add 100.0mL of sterile glucoseuric acid solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation and maintenance of Bacillus species.

Uric Acid Broth for Clostridia Composition per liter:

Preparation of Medium: Add components, except glucose-uric acid solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 100.0mL of sterile glucose-uric acid solution. Mix thoroughly. Use: For the cultivation and maintenance of Bacillus species.

K2HPO4......................................................................................... 4.0g Uric acid........................................................................................ 3.0g Yeast extract.................................................................................. 1.0g Sodium thioglycolate .................................................................... 0.5g MgSO4·7H2O ................................................................................ 0.1g FeSO4·7H2O............................................................................... 5.0mg Phenol Red (0.04% solution).....................................................1.0mL pH 7.6–8.0 at 25°C

Uric Acid Agar for Clostridia

Preparation of Medium: Add components, except uric acid, to ap-

Composition per liter: Agar ............................................................................................ 20.0g K2HPO4 ......................................................................................... 4.0g Uric acid........................................................................................ 3.0g Yeast extract.................................................................................. 1.0g Sodium thioglycolate .................................................................... 0.5g MgSO4·7H2O ................................................................................ 0.1g FeSO4·7H2O............................................................................... 5.0mg Phenol Red (0.04% solution) .....................................................1.0mL pH 7.6–8.0 at 25°C

Preparation of Medium: Add components, except uric acid, to ap-

proximately 900.0mL of distilled/deionized water. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.6 with 1N NaOH. Add the uric acid. Mix thoroughly. Adjust pH to 7.6. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of anaerobic bacteria, such as Clostridium acidurici and Clostridium cylindrosporum, that can utilize uric acid as the sole source of carbon and energy.

Uric Acid Medium Composition per liter:

Use: For the cultivation and maintenance of anaerobic bacteria, such

Agar ............................................................................................ 15.0g Peptone ......................................................................................... 5.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 2.0g Beef extract................................................................................... 1.0g Uric acid solution...................................................................150.0mL

as Clostridium acidurici and Clostridium cylindrosporum, that can utilize uric acid as the sole source of carbon and energy.

Uric Acid Solution: Composition per 150.0mL:

Uric Acid Broth Composition per liter: Na2HPO4·12H2O........................................................................... 9.0g NaCl .............................................................................................. 5.0g KH2PO4 ......................................................................................... 1.5g Meat extract .................................................................................. 1.0g Yeast extract.................................................................................. 1.0g MgSO4·7H2O................................................................................ 0.2g MnCl2·4H2O ............................................................................ 20.0mg CaCl2 .......................................................................................... 1.2mg Glucose-uric acid solution .....................................................100.0mL © 2010 by Taylor and Francis Group, LLC

Uric acid........................................................................................ 6.0g

Preparation of Uric Acid Solution: Add uric acid to distilled/deionized water and bring volume to 150.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Preparation of Medium: Add components, except uric acid solution, to distilled/deionized water and bring volume to 750.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 150.0mL of sterile uric acid solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes while agitating.

Use: For the cultivation of Bacillus fastidiosus.

1874

Uric Acid Semisolid Agar for Clostridia

Preparation of Urea Solution: Add urea to distilled/deionized wa-

Composition per liter:

ter and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

K2HPO4 ......................................................................................... 4.0g Uric acid........................................................................................ 3.0g Agar .............................................................................................. 2.0g Yeast extract.................................................................................. 1.0g Sodium thioglycolate .................................................................... 0.5g MgSO4·7H2O ................................................................................ 0.1g FeSO4·7H2O............................................................................... 5.0mg Phenol Red (0.04% solution) .....................................................1.0mL pH 7.6–8.0 at 25°C

Vitamain Solution: Composition per 10.0mL:

Preparation of Medium: Add components, except uric acid, to approximately 900.0mL of distilled/deionized water. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.6 with 1N NaOH. Add the uric acid. Mix thoroughly. Adjust pH to 7.6. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Clostridium acidurici and Clostridium cylindrosporum that can utilize uric acid.

Uric Acid Utilization Agar Composition per liter: Agar ............................................................................................ 15.0g Uric acid...................................................................................... 10.0g K2HPO4 ......................................................................................... 0.5g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

Glucose ......................................................................................... 2.0g L-Cysteine·HCl ......................................................................... 0.518g L-Glutamine .................................................................................. 0.2g L-Cystine................................................................................... 0.022g Adenine sulfate ........................................................................... 0.02g Nicotinamide adenine dinucleotide ........................................... 5.0mg Cocarboxylase............................................................................ 2.0mg Guanine·HCl .............................................................................. 0.6mg Fe(NO3)3·6H2O.......................................................................... 0.4mg p-Aminobenzoic acid............................................................... 0.26mg Vitamin B12 ................................................................................ 0.2mg Thiamine·HCl .......................................................................... 0.06mg

Preparation of Vitamin solution: Add components to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except vitamin solution, urea solution, horse serum, and selective supplement solution, to distilled/deionized water and bring volume to 920.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add vitamin solution, urea solution, horse serum, and selective supplement solution. Mix thoroughly. Aseptically distribute into sterile tubes.

Use: For the selective culture of Mycoplasma hominis and Ureaplasma urealyticum.

Use: For the cultivation and maintenance of anaerobic bacteria, such

USP Alternative Thioglycolate Medium See: Sterility Test Broth

as Bacillus fastidiosus, that can utilize uric acid as the sole source of carbon and energy.

Urogenital Mycoplasma Broth Base Composition per liter: Heart infusion powder .................................................................. 8.0g Casein enzymatic hydrolysate ...................................................... 8.0g Yeast extract.................................................................................. 4.0g NaCl .............................................................................................. 3.5g Arginine hydrochloride................................................................. 5.0g Cysteine hydrochloride ................................................................. 0.1g Phenol Red .................................................................................. 0.05g Horse serum ..............................................................................50.0ml Urea solution............................................................................10.0mL Vitamin solution.......................................................................10.0mL Selective supplement solution .................................................10.0mL pH 6.3 ± 0.2 at 25°C

Source: This medium is available from HiMedia. Selective Supplement Solution: Composition per 10.0mL: Penicillin .................................................................................... 5.0mg Amphotericin B.......................................................................... 1.0mg Penicillin .............................................................................. 100,000U

Preparation of Selective Supplement Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Ustilago Complete Agar II Composition per liter: Agar ............................................................................................ 20.0g Glucose ....................................................................................... 10.0g Hydrolyzed casein ........................................................................ 2.5g NH4NO3 ........................................................................................ 1.5g Yeast extract.................................................................................. 1.0g Salt solution .............................................................................62.5mL Vitamin solution.......................................................................10.0mL Hydrolyzed nucleic acids solution.............................................5.0mL pH 7.0 ± 0.2 at 25°C

Salt Solution: Composition per liter: KH2PO4....................................................................................... 16.0g KCl................................................................................................ 8.0g Na2SO4 .......................................................................................... 4.0g MgSO4 .......................................................................................... 2.0g CaCl2 ............................................................................................. 1.0g Trace elements solution .............................................................8.0mL

Trace Elements Solution: Composition per liter: CuSO4·5H2O ................................................................................. 0.4g ZnCl2 ............................................................................................. 0.4g MnCl2·4H2O ............................................................................... 0.14g FeCl3·6H2O ............................................................................ 100.0mg H3BO3 ...................................................................................... 60.0mg Na2MoO4·2H2O ....................................................................... 40.0mg

Ustilago Medium Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Salt Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Vitamin Solution: Composition per liter: Inositol .......................................................................................... 1.0g Calcium pantothenate ................................................................... 0.2g Choline chloride............................................................................ 0.2g Nicotinic acid ................................................................................ 0.2g Thiamine ................................................................................ 100.0mg p-Aminobenzoic acid............................................................... 50.0mg Pyridoxine ................................................................................ 50.0mg Riboflavin ................................................................................ 50.0mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Hydrolyzed Nucleic Acids Solution: Composition per 80.0mL: DNA, calf thymus ......................................................................... 2.0g RNA .............................................................................................. 2.0g HCl (1M solution) ....................................................................30.0mL NaOH (1M solution) ................................................................30.0mL

Use: For the cultivation and maintenance of Ustilago species.

Ustilago Complete Broth II Composition per liter: Glucose ....................................................................................... 10.0g Hydrolyzed casein......................................................................... 2.5g NH4NO3 ........................................................................................ 1.5g Yeast extract.................................................................................. 1.0g Salt solution .............................................................................62.5mL Vitamin solution.......................................................................10.0mL Hydrolyzed nucleic acids solution .............................................5.0mL pH 7.0 ± 0.2 at 25°C

Salt Solution: Composition per liter: KH2PO4 ....................................................................................... 16.0g KCl................................................................................................ 8.0g Na2SO4 .......................................................................................... 4.0g MgSO4 .......................................................................................... 2.0g CaCl2 ............................................................................................. 1.0g Trace elements solution .............................................................8.0mL

1875

FeCl3·6H2O............................................................................ 100.0mg H3BO3 ...................................................................................... 60.0mg Na2MoO4·2H2O ....................................................................... 40.0mg

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Salt Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Vitamin Solution: Composition per liter: Inositol .......................................................................................... 1.0g Calcium pantothenate ................................................................... 0.2g Choline chloride............................................................................ 0.2g Nicotinic acid................................................................................ 0.2g Thiamine ................................................................................ 100.0mg p-Aminobenzoic acid............................................................... 50.0mg Pyridoxine................................................................................ 50.0mg Riboflavin ................................................................................ 50.0mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Hydrolyzed Nucleic Acids Solution: Composition per 80.0mL: DNA, calf thymus......................................................................... 2.0g RNA.............................................................................................. 2.0g HCl (1M solution)....................................................................30.0mL NaOH (1M solution) ................................................................30.0mL

Preparation of Hydrolyzed Nucleic Acids Solution: Add DNA to 30.0mL of 1M NaOH solution. Add RNA to 30.0mL of 1M HCl solution. Autoclave the two solutions separately for 10 min at 15 psi pressure–121°C. Mix the two solutions. Adjust the pH to 6.0. Centrifuge at 5000 × g for 10 min. Decant supernatant solution and filter. Bring volume to 80.0mL with distilled/deionized water. Store at −20°C. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation of Ustilago species.

Ustilago Medium Composition per liter: Yeast extract................................................................................ 11.0g Glucose ....................................................................................... 10.0g NH4NO3 ........................................................................................ 1.5g Salt solution .............................................................................62.5mL Vitamin solution.......................................................................10.0mL

Salt Solution: Composition per liter: KH2PO4....................................................................................... 16.0g KCl................................................................................................ 8.0g Na2SO4 .......................................................................................... 4.0g MgSO4·7H2O ................................................................................ 2.0g CaCl2 ............................................................................................. 1.0g Trace elements solution .............................................................8.0mL

Preparation of Salt Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

1876

Ustilago Medium

MnCl2·4H2O................................................................................ 0.07g FeCl3·6H2O ................................................................................. 0.05g H3BO3 ......................................................................................... 0.03g Na2MoO4·2H2O .......................................................................... 0.02g

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly.

Vitamin Solution: Composition per liter: Inositol .......................................................................................... 0.4g Calcium pantothenate ................................................................... 0.2g Choline chloride............................................................................ 0.2g Nicotinic acid ................................................................................ 0.2g Thiamine ....................................................................................... 0.1g Pyridoxine ................................................................................... 0.05g Riboflavin ................................................................................... 0.05g

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except vitamin solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile vitamin solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Ustilago species.

Ustilago Medium Composition per liter: Agar ............................................................................................ 20.0g Glucose ....................................................................................... 10.0g Peptone........................................................................................ 10.0g Malt extract ................................................................................... 3.0g Yeast extract.................................................................................. 3.0g Beef extract ................................................................................... 1.0g pH 5.7 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 5.7. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Ustilago species.

Ustilago Minimal Medium Composition per liter: Glucose ....................................................................................... 10.0g KNO3 ............................................................................................ 3.0g Salt solution .............................................................................62.5mL

Salt Solution: Composition per liter: KH2PO4 ....................................................................................... 16.0g KCl................................................................................................ 8.0g Na2SO4 .......................................................................................... 4.0g MgSO4·7H2O ................................................................................ 2.0g CaCl2 ............................................................................................. 1.0g Trace elements solution .............................................................8.0mL

Trace Elements Solution: Composition per 500.0mL: CuSO4·5H2O ................................................................................. 0.2g ZnCl2 ............................................................................................. 0.2g MnCl2·4H2O ............................................................................... 0.07g FeCl3·6H2O ................................................................................. 0.05g H3BO3 ......................................................................................... 0.03g Na2MoO4·2H2O .......................................................................... 0.02g

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Ustilago species.

UVM Listeria Enrichment Broth (University of Vermont Listeria Enrichment Broth) Composition per liter: NaCl............................................................................................ 20.0g Na2HPO4 ....................................................................................... 9.6g Pancreatic digest of casein............................................................ 5.0g Peptic digest of animal tissue ....................................................... 5.0g Beef extract................................................................................... 5.0g Yeast extract.................................................................................. 5.0g KH2PO4....................................................................................... 1.35g Esculin .......................................................................................... 1.0g Nalidixic acid........................................................................... 40.0mg Acriflavine·HCl ....................................................................... 12.0mg pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the selective isolation of Listeria monocytogenes.

UVM Modified Listeria Enrichment Broth (University of Vermont Modified Listeria Enrichment Broth) Composition per liter: NaCl............................................................................................ 20.0g Na2HPO4 ....................................................................................... 9.6g Pancreatic digest of casein............................................................ 5.0g Peptic digest of animal tissue ....................................................... 5.0g Beef extract................................................................................... 5.0g Yeast extract.................................................................................. 5.0g KH2PO4....................................................................................... 1.35g Esculin .......................................................................................... 1.0g Nalidixic acid........................................................................... 20.0mg Acriflavine·HCl ....................................................................... 12.0mg pH 7.2 ± 0.2 at 25°C

Use: For the selective isolation of Listeria monocytogenes.

V-8™-0 Agar

V Agar Composition per liter: Agar ............................................................................................ 13.5g Pancreatic digest of casein .......................................................... 12.0g Peptone........................................................................................ 10.0g Peptic digest of animal tissue........................................................ 5.0g NaCl .............................................................................................. 5.0g Beef extract ................................................................................... 3.0g Yeast extract.................................................................................. 3.0g Cornstarch ..................................................................................... 1.0g Human blood, anticoagulated ..................................................50.0mL pH 7.4 ± 0.2 at 25°C

1877

V-8™ Agar, Half-strength (ATCC Medium 2216) Composition per liter:

agnostic Systems.

Agar ............................................................................................ 15.0g CaCO3 ......................................................................................... 0.75g V-8™ canned vegetable juice ..................................................50.0mL pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except human blood, to

Preparation of Medium: Add components to 950.0mL distilled/

Source: This medium is available as a prepared medium from BD Di-

distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 50.0mL of human blood. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation and differentiation of Gardnerella vaginalis from clinical specimens. Plates are incubated under an atmosphere with 3–10% CO2. Gardnerella vaginalis appears as small white colonies with diffuse β-hemolysis.

V-8™ Agar Composition per liter: Agar ............................................................................................ 20.0g CaCO3 ........................................................................................... 4.0g V-8™ canned vegetable juice ................................................200.0mL pH 7.3 ± 0.2 at 25°C

Use: For the isolation and cultivation of Actinomadura species, Actinopolyspora species, Excellospora species, and Microspora species.

V-8™ Agar Composition per liter: Agar ............................................................................................ 15.0g CaCO3 ........................................................................................... 2.0g V-8™ canned vegetable juice ................................................200.0mL

Use: For the cultivation of numerous yeasts and filamentous fungi.

V-8™ Agar, Half-strength (ATCC Medium 2211) Composition per liter: Agar ............................................................................................ 15.0g CaCO3 ........................................................................................... 1.5g V-8™ canned vegetable juice ................................................100.0mL pH 7.2 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

deionized water. Mix thoroughly. Bring volume to 1.0L with tap water. Mix thoroughly. Adjust to pH 7.2 ± 0.2. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation and cultivation of Actinomadura species, Actinopolyspora species, Excellospora species, and Microspora species.

V-8™ Agar, pH 7.2 Composition per liter: Agar ............................................................................................ 15.0g CaCO3 ........................................................................................... 3.0g V-8™ canned vegetable juice ................................................200.0mL pH 7.2 ± 0.2 at 25°C

Use: For the cultivation of Bipolaris leersiae, Bipolaris micropus, Camposporium pellucidum, Cochliobolus spicifer, Cochliobolus bicolor, Cochliobolus miyabeanus, Cochliobolus australiensis, Cochliobolus sativus, Cochliobolus victoriae, Curvularia inaequalis, Drechslera catenaria, Drechslera panicimiliacei, Embellisia chlamydospora, Helicosporium pallidum, Helminthosporium papulosum, Phytophthora cinnamomi, Phytophthora cryptogea, Setosphaeria rostrata, and other yeasts and filamentous fungi.

V-8™-0 Agar Composition per liter: Agar ........................................................................................... 15.0 g CaCO3 .......................................................................................... 5.0 g CaCl3 ...................................................................................... 100.0mg β-Sitosterol .............................................................................. 30.0mg Tryptophan............................................................................... 20.0mg Thiamine .................................................................................... 1.0mg V-8™ canned vegetable juice ................................................354.0mL

Preparation of Medium: Add CaCO3 to V-8. Centrifuge for 20 min

at 4000 rpm. Decant the supernatant. Add the supernatant to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Add remaining components. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

1878

V-8™ Juice Agar

Use: For the cultivation of Phytophthora syringae, Phytophthora palmivora, Phytophthora nicotianae, Phytophthora erythroseptica, Phytophthora drechsleri, and Phytophthora citrophthora.

V-8™ Juice Agar (ATCC Medium 343) Composition per liter: Agar ............................................................................................ 15.0g CaCO3 ........................................................................................... 4.0g V-8™ canned vegetable juice ................................................200.0mL pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.2. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation and cultivation of Filobasidiella depauperata and Leucosporidium scottii.

V-8™ Juice Agar II (ATCC Medium 2040) Composition per liter: Agar ............................................................................................ 30.0g V-8™ canned vegetable juice ................................................300.0mL pH 7.3± 0.2 at 25°C

Use: For the cultivation of various yeasts and filamentous fungi.

V-8™ Juice Seawater Agar Composition per liter: Agar ............................................................................................ 15.0g CaCO3 ........................................................................................... 3.0g Artificial seawater..................................................................800.0mL V-8™ canned vegetable juice, unsalted ................................ 200.0mL pH 7.0 ± 0.2 at 25°C

Artificial Seawater: Composition per liter: NaCl ............................................................................................ 20.0g MgCl2·6H2O................................................................................ 5.38g MgSO4·7H2O .............................................................................. 6.78g KCl.............................................................................................. 0.72g NaHCO3 ....................................................................................... 0.2g CaCl2·2H2O................................................................................... 1.4g

Preparation of Artificial Seawater: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly.

V-8™ Rye Agar Composition per 1050.0mL: Agar ............................................................................................ 20.0g CaCO3 .......................................................................................... 0.2g Rye broth.......................................................................................1.0L V-8™ canned vegetable juice ..................................................50.0mL

Rye Broth: Composition per liter: Whole rye grains......................................................................... 50.0g

Preparation of Rye Broth: Soak the rye grains in 1.1L of distilled/ deionized water for 24–36 hr at 24°C. Autoclave at 15 psi pressure– 121°C for 30 min. Filter through four layers of cheesecloth. Bring the final filtrate volume to 1.0L with distilled/deionized water.

Use: For the cultivation and maintenance of Phytophthora infestans.

V24N Medium (DSMZ Medium 88b) Composition per liter: Starch, soluble............................................................................... 2.0g (NH4)2SO4 .................................................................................... 1.3g Sulfur, powdered........................................................................... 1.0g Na2S·9H2O.................................................................................... 0.5g KH2PO4....................................................................................... 0.28g MgSO4·7H2O .............................................................................. 0.25g Yeast extract.................................................................................. 0.2g CaCl2·2H2O ................................................................................ 0.07g FeCl3·6H2O ................................................................................. 0.02g Na2B4O7·10H2O......................................................................... 4.5mg MnCl2·4H2O .............................................................................. 1.8mg Resazurin ................................................................................... 0.4mg ZnSO4·7H2O ............................................................................ 0.22mg CuCl2·2H2O ............................................................................. 0.05mg Na2MoO4·2H2O ...................................................................... 0.03mg VOSO4·2H2O........................................................................... 0.03mg CoSO4 ...................................................................................... 0.01mg pH 6.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust the pH to 6.0 with H2SO4 (25% v/v). Sparge medium with 100% N2 gas. Distribute anaerobically into rubber-stoppered tubes or bottles that have been presterilized by autoclaving. On 2 successive days heat the medium for 2 hr at 85 °C.

Use: For the growth and maintenance of Thermofilum librum.

Van Niel’s Agar Composition per liter:

Adjust pH to 7.0. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Agar ............................................................................................ 20.0g Yeast extract................................................................................ 10.0g K2HPO4......................................................................................... 1.0g MgSO4 .......................................................................................... 0.5g pH 7.0 ± 0.2 at 25°C

Use: For the cultivation of Halophytophthora masteri and Halophy-

Preparation of Medium: Add components to distilled/deionized

tophthora tartarea.

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

Preparation of Medium: Combine components. Mix thoroughly.

Van Niel’s Yeast Medium with Pyruvate, Modified

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Rhodomicrobium vannielii.

Van Niel’s Medium, Modified Composition per liter: Yeast extract................................................................................ 10.0g MgSO4 .......................................................................................... 0.1g EDTA ......................................................................................... 2.0mg Trace elements solution ...........................................................10.0mL K2HPO4 solution........................................................................2.5mL pH 7.1 ± 0.2 at 25°C

Trace Elements Solution: Composition per 100.0mL: CaCl2·2H2O................................................................................... 0.3g Ferric ammonium citrate............................................................... 0.2g

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

K2HPO4 Solution: Composition per 100.0mL: K2HPO4 ......................................................................................... 4.0g

Preparation of K2HPO4 Solution: Add K2HPO4 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except K2HPO4 and

trace elements solution, to distilled/deionized water and bring volume to 987.5mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 50°C. Aseptically add trace elements and K2HPO4 solutions. Mix thoroughly. Distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Rhodobacter sphaeroides. Van Niel’s Yeast Agar See: Van Niel’s Agar

Van Niel’s Yeast Agar with Glutamate (ATCC Medium 1370) Composition per liter: Agar ............................................................................................ 20.0g Yeast extract................................................................................ 10.0g K2HPO4 ......................................................................................... 1.0g Glutamate...................................................................................... 0.7g MgSO4 .......................................................................................... 0.5g pH 7.1 ± 0.2 at 25°C

Van Niel’s Yeast Agar with Sodium Chloride Composition per liter: NaCl ............................................................................................ 25.0g Agar ............................................................................................ 20.0g Yeast extract................................................................................ 10.0g K2HPO4 ......................................................................................... 1.0g MgSO4 .......................................................................................... 0.5g pH 7.1 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

1879

Van Niel’s Yeast Agar with 25% Sodium Chloride (ATCC Medium 217) Composition per liter: NaCl.......................................................................................... 250.0g Agar ............................................................................................ 20.0g Yeast extract................................................................................ 10.0g K2HPO4......................................................................................... 1.0g MgSO4 .......................................................................................... 0.5g pH 7.1 ± 0.2 at 25°C

Van Niel’s Yeast Agar with Succinate (ATCC Medium 1243) Composition per liter: Agar ............................................................................................ 20.0g Yeast extract................................................................................ 10.0g K2HPO4......................................................................................... 1.0g Succinate....................................................................................... 0.6g MgSO4 .......................................................................................... 0.5g pH 7.1 ± 0.2 at 25°C

Van Niel’s Yeast Medium with Pyruvate, Modified Composition per liter: Yeast extract................................................................................ 10.0g MgSO4 .......................................................................................... 0.1g EDTA ......................................................................................... 2.0mg Sodium pyruvate solution ......................................................100.0mL Trace elements solution ...........................................................10.0mL K2HPO4 solution........................................................................5.0mL Trace metal A-5 solution ...........................................................1.0mL pH 7.1± 0.1 at 25°C

Sodium Pyruvate Solution: Composition per 100.0mL: Sodium pyruvate........................................................................... 1.1g

Preparation of Sodium Pyruvate Solution: Add sodium pyruvate to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Trace Elements Solution: Composition per 100.0mL: CaCl2·2H2O .................................................................................. 0.3g Ferric ammonium citrate............................................................... 0.2g

1880

Vanillate Medium

Preparation of Trace Elements Solution: Add components to

Preparation of Vanillic Acid Solution: Add vanillic acid to dis-

distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

K2HPO4 Solution: Composition per 100.0mL:

Preparation of Medium: Add components, except vanillic acid so-

K2HPO4 ......................................................................................... 4.0g

Preparation of K2HPO4 Solution: Add K2HPO4 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Trace Metal A-5 Solution: Composition per liter: H3BO3 ......................................................................................... 2.86g MnCl2·4H2O................................................................................ 1.81g Na2MoO4·2H2O .......................................................................... 0.39g ZnSO4·7H2O ............................................................................. 0.222g CuSO4·5H2O ............................................................................. 0.079g Co(NO3)2·6H2O ........................................................................ 0.049g

Preparation of Trace Metal A-5 Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except sodium pyruvate, trace elements, K2HPO4, and trace metal A-5 solutions, to distilled/deionized water and bring volume to 884.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 100.0mL of sterile sodium pyruvate solution, 10.0mL of sterile trace elements solution, 5.0mL of sterile K2HPO4 solution, and 1.0mL of sterile trace metal A-5 solution. Mix thoroughly. Adjust pH to 7.1 ± 0.1. Aseptically distribute into sterile tubes or flasks.

lution and trace elements solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°– 55°C. Warm the vanillic acid solution and the trace elements solution to 50°–55°C. Aseptically add 10.0mL of sterile vanillic acid solution and 10.0mL of sterile trace elements solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Pseudomonas fluorescens.

VCR Medium Composition per 1001.0mL: Agar, noble.................................................................................. 15.0g NaNO3 .......................................................................................... 2.0g KH2PO4......................................................................................... 1.5g K2HPO4......................................................................................... 1.2g NH4Cl ........................................................................................... 0.5g MgSO4·7H2O ................................................................................ 0.2g FeCl3 ........................................................................................... 0.01g CaCl2·2H2O ............................................................................. 15.0mg CuSO4·5H2O .............................................................................. 1.0mg Vitamin B12 solution ..................................................................1.0mL pH 7.2 ± 0.2 at 25°C

Vitamin B12 Solution: Composition per 10.0mL: Vitamin B12 ............................................................................... 10.0μg

Use: For the cultivation and maintenance of photosynthetic bacteria, such as Heliobacillus mobilis and Rhodopseudomonas palustris.

Preparation of Vitamin B12 Solution: Add vitamin B12 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Vanillate Medium

Preparation of Medium: Add components, except vitamin B12 so-

Composition per liter: Agar ............................................................................................ 20.0g (NH4)2SO4 ..................................................................................... 1.0g KH2PO4 ......................................................................................... 0.4g Yeast extract.................................................................................. 0.1g MgSO4·7H2O .............................................................................. 0.01g Trace elements solution ...........................................................10.0mL Vanillic acid solution ...............................................................10.0mL

Trace Elements Solution: Composition per liter: MnSO4·4H2O ................................................................................ 0.4g H3BO3 ........................................................................................ 0.5mg ZnSO4·7H2O .............................................................................. 0.4mg FeCl3 .......................................................................................... 0.2mg (NH4)6Mo7O24·4H2O ................................................................. 0.2mg KI ............................................................................................... 0.1mg CuSO4·5H2O ............................................................................ 0.04mg

Preparation of Trace Elements Solution: Add components to

lution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 1.0mL of sterile vitamin B12 solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Sphaerobacter thermomethanica.

Veal Infusion Agar Composition per liter: Agar ............................................................................................ 15.0g Veal, infusion from ..................................................................... 10.0g Pancreatic digest of casein............................................................ 5.0g Peptic digest of animal tissue ....................................................... 5.0g NaCl.............................................................................................. 5.0g pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized

distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Vanillic Acid Solution: Composition per 10.0mL:

Use: For the cultivation and maintenance of a variety of microorgan-

isms. Can be used for the cultivation of fastidious microorganisms when enriched with blood or serum.

Veal Infusion Broth with Rabbit Serum

Veal Infusion Agar (ATCC Medium 521)

1881

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Composition per liter:

Preparation of Medium: Add components to distilled/deionized

Veal, infusion from ................................................................... 500.0g Agar ............................................................................................ 15.0g Pancreatic digest of casein ............................................................ 5.0g Peptic digest of animal tissue........................................................ 5.0g NaCl .............................................................................................. 5.0g pH 7.4 ± 0.2 at 25°C

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use freshly prepared solution.

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Use: For the cultivation and maintenance of a variety of microorganisms. Can be used for the cultivation of fastidious microorganisms when enriched with blood or serum.

Veal Infusion Agar (BAM M173)

Use: For the cultivation and maintenance of Arthrobacter species, streptococci, and other microorganisms.

Veal Infusion Broth (BAM M173) Composition per liter: Veal, infusion from ................................................................... 500.0g Proteose peptone No.3 ................................................................ 10.0g NaCl.............................................................................................. 5.0g pH 7.4 ± 0.2 at 25°C

Source: This medium is available from BD Diagnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Composition per liter:

Use: For the cultivation of fastidious microorganisms.

Veal, infusion from ................................................................... 500.0g Agar ............................................................................................ 15.0g Proteose peptone No. 3 ............................................................... 10.0g NaCl .............................................................................................. 5.0g pH 7.3 ± 0.2 at 25°C

Composition per liter:

Source: This medium is available from BD Diagnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Pour into sterile Petri dishes or leave in tubes. For slants allow tubes to cool in an inclined position.

Use: For the cultivation of fastidious microorganisms.

Veal Infusion Broth Composition per liter: Veal, infusion from ..................................................................... 10.0g Pancreatic digest of casein ............................................................ 5.0g Peptic digest of animal tissue........................................................ 5.0g NaCl .............................................................................................. 5.0g pH 7.4 ± 0.2 at 25°C

Source: This medium is available from BD Diagnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Use freshly prepared solution.

Use: For the cultivation of streptococci and other microorganisms.

Veal Infusion Broth (ATCC Medium 521) Composition per liter: Veal, infusion from ................................................................... 500.0g Pancreatic digest of casein ............................................................ 5.0g Peptic digest of animal tissue........................................................ 5.0g NaCl .............................................................................................. 5.0g pH 7.4 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

Veal Infusion Broth with Horse Serum Veal, infusion from ................................................................... 500.0g Pancreatic digest of casein............................................................ 5.0g Peptic digest of animal tissue ....................................................... 5.0g NaCl.............................................................................................. 5.0g Horse serum, heat inactivated................................................100.0mL pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components, except horse serum, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 100.0mL of horse serum. Mix thoroughly. Aseptically distribute into tubes or flasks. Use freshly prepared solution or boil without mixing prior to use. Use: For the cultivation and maintenance of Streptococcus pyogenes.

Veal Infusion Broth with Rabbit Serum Composition per liter: Veal, infusion from ................................................................... 500.0g Pancreatic digest of casein............................................................ 5.0g Peptic digest of animal tissue ....................................................... 5.0g NaCl.............................................................................................. 5.0g Rabbit serum, heat inactivated...............................................150.0mL pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components, except rabbit serum, to distilled/deionized water and bring volume to 850.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 150.0mL of rabbit serum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Boil without agitation prior to use. Use freshly prepared solution or boil without mixing prior to use. Use: For the cultivation and maintenance of Proteus mirabilis. Veal Yeast Extract Medium See: VY Medium

1882

Veillonella Agar

Veillonella Agar Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein ............................................................ 5.0g Yeast extract.................................................................................. 3.0g Sodium thioglycolate .................................................................. 0.75g Vancomycin ............................................................................... 7.5mg Basic Fuchsin............................................................................. 2.0mg Sodium lactate (60% solution).................................................21.0mL pH 7.5± 0.2 at 25°C

Caution: Basic Fuchsin is a potential carcinogen and care must be taken to avoid inhalation of the powdered dye and contact with the skin.

Selective Supplement Solution: Composition per 10.0mL: Vancomycin ............................................................................... 7.5mg

Preparation of Selective Supplement Solution: Add vancomycin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Caution: Basic Fuchsin is a potential carcinogen and care must be taken to avoid inhalation of the powdered dye and contact with the skin.

Preparation of Medium: Add components, except selective supplement solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL sterile selective supplement solution. Mix thoroughly. Pour into sterile Petri dishes or aseptically distribute into sterile tubes or flasks.

Preparation of Medium: Add components to distilled/deionized

Use: For the selective isolation and cultivation of Veillonella species.

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Use: For the isolation and cultivation of Veillonella species.

Veillonella HiVeg Agar Base with Lactate Composition per liter: Agar ............................................................................................ 15.0g Plant hydrolysate........................................................................... 5.0g Yeast extract.................................................................................. 3.0g Na-thioglycolate.......................................................................... 0.75g Basic Fuchsin............................................................................. 2.0mg Sodium lactate (60% solution).................................................21.0mL pH 7.5± 0.2 at 25°C

Source: This medium, without lactate solution, is available as a pre-

Veillonella Medium Composition per liter: Pancreatic digest of casein............................................................ 5.0g Yeast extract.................................................................................. 3.0g Tween™ 80................................................................................... 1.0g Glucose ......................................................................................... 1.0g Sodium thioglycolate .................................................................. 0.75g Sodium lactate (60% solution).................................................21.0mL pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5 with K2CO3. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Veillonella species.

mixed powder from HiMedia.

Caution: Basic Fuchsin is a potential carcinogen and care must be taken to avoid inhalation of the powdered dye and contact with the skin.

Use: For the selective isolation and cultivation of Veillonella species.

Veillonella HiVeg Agar Base with Lactate and Vancomycin Composition per liter: Agar ............................................................................................ 15.0g Plant hydrolysate........................................................................... 5.0g Yeast extract.................................................................................. 3.0g Na-thioglycolate.......................................................................... 0.75g Basic Fuchsin............................................................................. 2.0mg Sodium lactate (60% solution).................................................21.0mL Selective supplement solution .................................................10.0mL pH 7.5± 0.2 at 25°C

Veillonella Medium, DSM Composition per liter: Sodium lactate (60% solution)...................................................... 7.5g Pancreatic digest of casein............................................................ 5.0g Yeast extract.................................................................................. 3.0g Tween™ 80................................................................................... 1.0g Glucose ......................................................................................... 1.0g Sodium thioglycolate .................................................................. 0.75g Putrescine................................................................................... 3.0mg Resazurin ................................................................................... 1.0mg pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Prepare and dispense medium anaerobically under 100% N2. Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5 with K2CO3. Anaerobically distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Veillonella parvula and other Veillonella species.

Veillonella Selective Medium Composition per liter: Pancreatic digest of casein............................................................ 5.0g Yeast extract.................................................................................. 3.0g Tween™ 80................................................................................... 1.0g Sodium thioglycolate .................................................................. 0.75g

Venenivibrio stagnispumantis Medium

Sodium lactate (50% solution).................................................25.0mL Streptomycin solution ..............................................................10.0mL pH 6.6 ± 0.2 at 25°C

Streptomycin Solution: Composition per 10.0mL: Streptomycin .............................................................................. 5.0mg

Preparation of Streptomycin Solution: Add streptomycin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except streptomycin solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 6.6 with K2CO3. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°– 50°C. Aseptically add sterile streptomycin solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Veillonella species.

VEN CHI2 Medium (DSMZ Medium 293a) Composition per liter: NaCl ............................................................................................ 20.0g MgCl2·6H2O.................................................................................. 3.0g KCl................................................................................................ 0.5g NH4Cl ......................................................................................... 0.25g KH2PO4 ......................................................................................... 0.2g CaCl2·2H2O................................................................................. 0.15g Resazurin ................................................................................... 1.0mg NaHCO3 solution .....................................................................10.0mL Na2-succinate solution .............................................................10.0mL Na2S·9H2O solution .................................................................10.0mL Vitamin solution.......................................................................10.0mL Quinic acid solution .................................................................10.0mL Trace elements solution SL-10 ..................................................1.0mL pH 7.2 ± 0.2 at 25°C

Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................. 0.36g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature. NaHCO3 Solution: Composition per 10.0mL: NaHCO3 ........................................................................................ 2.5g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Filter sterilize. Na2-succinate Solution: Composition per 10.0mL: Na2-succinate .............................................................................. 3.25g

Preparation of Na2-succinate Solution: Add Na2-succinate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

1883

Riboflavin .................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg D-Ca-pantothenate ..................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize. Quinic Acid Solution: Composition per 10.0mL: Quinic acid.................................................................................... 1.0g

Preparation of Quinic Acid Solution: Add quinic acid to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Adjust pH to 7.0. Sparge with 80% N2 + 20% CO2. Filter sterilize.

Trace Elements Solution SL-10: Composition per liter: FeCl2·4H2O................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O.............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Filter sterilize.

Preparation of Medium: Prepare and dispense medium under 80% N2 + 20% CO2 gas atmosphere. Add components, except NaHCO3 solution, Na2-succinate solution, Na2S·9H2O solution, vitamin solution, quinic acid solution, and trace elements solution SL-10, to distilled/deionized water and bring volume to 949.0mL. Mix thoroughly. Adjust pH to 7.2. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add 10.0mL NaHCO3 solution, 10.0mL Na2-succinate solution, 10.0mL Na2S·9H2O solution, 10.0mL vitamin solution, 10.0mL quinic acid solution, and 1.0mL trace elements solution SL-10. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or bottles. After inoculation, flush and repressurize the gas head space of culture bottles with sterile 80% N2 + 20% CO2 to 1 bar overpressure.

Use: For the cultivation of Ilyobacter insuetus.

Venenivibrio stagnispumantis Medium (DSMZ Medium 1146) Composition per liter: MgSO4·7H2O ................................................................................ 7.0g Na2S2O3·5H2O .............................................................................. 2.0g MES ............................................................................................ 1.95g KCl................................................................................................ 0.5g MgCl2·6H2O ................................................................................. 0.4g CaCl2·2H2O .................................................................................. 0.4g NaOH.......................................................................................... 1.36g NH4Cl ........................................................................................... 0.2g

1884

Vibrio Agar

KH2PO4 ....................................................................................... 0.25g Trace elements solution ...........................................................10.0mL pH 5.5 ± 0.2 at 25°C

Trace Elements Solution: Composition per liter: EDTA ............................................................................................ 5.0g CoCl2·6H2O ........................................................................... 150.0mg MnCl2·4H2O........................................................................... 100.0mg ZnCl2 ...................................................................................... 100.0mg FeSO4·7H2O........................................................................... 100.0mg AlCl3·6H2O .............................................................................. 40.0mg Na2WO4·2H2O ......................................................................... 30.0mg CuCl2·2H2O ............................................................................. 20.0mg NiSO4·6H2O............................................................................. 20.0mg NaHSeO3.................................................................................. 10.0mg H3BO3 ...................................................................................... 10.0mg Na2MoO4·2H2O ....................................................................... 10.0mg

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Adjust pH to 3.0 with HCl. Mix thoroughly.

Preparation of Medium: Prepare anaerobic distilled/deionized water by sparging with with 100% CO2. Adjust pH to 5.5. Sparge with CO2 for 15 min. Add components to the distilled/deionized anaerobic water and bring volume to 1.0L. Dispense into culture tubes. Stopper and seal tubes by crimping caps onto stoppers. Autoclave for 20 min at 15 psi pressure–121°C. Use: For the cultivation of Venenivibrio stagnispumantis. Viability-Preserving Microbiostatic Medium See: VMGII Medium

Vibrio Agar Composition per liter: Sucrose........................................................................................ 20.0g Agar ............................................................................................ 15.0g NaCl ............................................................................................ 10.0g Sodium citrate·2H2O................................................................... 10.0g Na2S2O3·5H2O .............................................................................. 6.5g Oxgall............................................................................................ 5.0g Yeast extract.................................................................................. 5.0g Pancreatic digest of casein ............................................................ 4.0g Proteose peptone ........................................................................... 3.0g Sodium deoxycholate.................................................................... 1.0g Sodium lauryl sulfate .................................................................... 0.2g Water Blue .................................................................................... 0.2g Cresol Red................................................................................... 0.02g pH 8.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.5. Gently heat and bring to boiling. Do not autoclave. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of the Vibrio cholerae.

Yeast extract................................................................................ 10.0g Proteose peptone........................................................................... 5.0g KCl................................................................................................ 2.0g Glucose ......................................................................................... 1.0g CaCl2·2H2O ................................................................................ 0.48g NaHCO3 ................................................................................... 60.0mg NaBr......................................................................................... 26.0mg

Use: For the cultivation and maintenance of Vibrio costicola.

Vibrio HiVeg Agar Composition per liter: Sucrose........................................................................................ 20.0g Agar ............................................................................................ 15.0g NaCl............................................................................................ 10.0g Sodium citrate·2H2O .................................................................. 10.0g Plant hydrolysate .......................................................................... 8.0g Na2S2O3·5H2O.............................................................................. 6.5g Yeast extract.................................................................................. 5.0g Plant peptone No. 3....................................................................... 3.0g Synthetic detergent No. II............................................................. 1.0g Synthetic detergent No. III............................................................ 1.0g Sodium lauryl sulfate.................................................................... 0.2g China Blue .................................................................................... 0.2g Cresol Red .................................................................................... 0.2g pH 8.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Use: For the isolation and cultivation of the Vibrio cholerae.

Vibrio Medium Composition per liter: NaCl............................................................................................ 10.0g Pancreatic digest of casein.......................................................... 10.0g MgCl2·6H2O ................................................................................. 4.0g KCl................................................................................................ 1.0g pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Vibrio diazotrophicus.

Vibrio natriegens Medium Composition per liter:

Vibrio costicola Medium Composition per liter: NaCl ............................................................................................ 81.0g MgSO4·7H2O .............................................................................. 19.7g Agar ............................................................................................ 15.0g MgCl·H2O ................................................................................... 15.0g © 2010 by Taylor and Francis Group, LLC

Urea............................................................................................. 20.0g NaCl............................................................................................ 15.0g Agar ............................................................................................ 15.0g Peptone ......................................................................................... 5.0g Meat extract .................................................................................. 3.0g pH 7.0 ± 0.2 at 25°C

Vibrio vulnificus Agar Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Vibrio natriegens.

Vibrio parahaemolyticus Agar (VP Agar) Composition per liter: Agar ............................................................................................ 20.0g NaCl ............................................................................................ 20.0g Sucrose........................................................................................ 20.0g Sodium citrate ............................................................................. 10.0g Na2S2O3·5H2O ............................................................................ 10.0g Peptone........................................................................................ 10.0g Sodium taurocholate ..................................................................... 5.0g Yeast extract.................................................................................. 5.0g Sodium lauryl sulfate .................................................................... 0.2g Bromthymol Blue ....................................................................... 0.04g Thymol Blue ............................................................................... 0.04g pH 8.6 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Do not autoclave. Pour into sterile Petri dishes.

Use: For the isolation, cultivation, enumeration, and presumptive identification of coliforms in milk, food, and other specimens of sanitary significance. For the enumeration of bacteria in cheese, especially Pseudomonas fragi, Pseudomonas viscosa, and Alcaligenes metalcaligenes. Sucrose-fermenting bacteria appear as yellow colonies with pale yellow peripheries. Sucrose-nonfermenting bacteria appear as mucoid, green colonies with a dark green center.

Vibrio parahaemolyticus Sucrose Agar (VPSA) Composition per liter: NaCl ............................................................................................ 30.0g Agar ............................................................................................ 15.0g Sucrose........................................................................................ 10.0g Yeast extract.................................................................................. 7.0g Tryptose ........................................................................................ 5.0g Pancreatic digest of casein ............................................................ 5.0g Bile salts No. 3.............................................................................. 1.5g Bromthymol Blue ..................................................................... 0.025g pH 8.6 ± 0.2 at 25°C

Use: For the isolation, cultivation, and differentiation of Vibrio parahaemolyticus from seafood. Vibrio parahaemolyticus and Vibrio vulnificus appear as blue to green colonies. Other Vibrio species appear as yellow colonies.

Vibrio parahaemolyticus Sucrose HiVeg Agar

1885

Sucrose........................................................................................ 10.0g Yeast extract.................................................................................. 7.0g Plant hydrolysate .......................................................................... 5.0g Plant hydrolysate No. 1................................................................. 5.0g Synthetic detergent No. I .............................................................. 1.5g Bromthymol Blue ..................................................................... 0.025g pH 8.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Do not autoclave. Cool to 50°C. Pour into sterile Petri dishes in 20.0mL volumes. Allow plates to dry before using. Use: For the isolation, cultivation, and differentiation of Vibrio parahaemolyticus from seafood. Vibrio parahaemolyticus and Vibrio vulnificus appear as blue to green colonies. Other Vibrio species appear as yellow colonies.

Vibrio vallismortis Medium Composition per liter: NaCl............................................................................................ 25.0g MgSO4·7H2O ................................................................................ 9.6g MgCl2·6H2O ................................................................................. 7.0g Glucose ......................................................................................... 5.0g KCl................................................................................................ 3.8g Yeast extract.................................................................................. 1.0g CaCl2·2H2O .................................................................................. 0.5g K2HPO4·3H2O .............................................................................. 0.4g NaHCO3 solution.....................................................................20.0mL pH 7.0 ± 0.2 at 25°C

NaHCO3 Solution: Composition per 20.0mL: NaHCO3 ........................................................................................ 3.0g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except NaHCO3 solu-

tion, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add 20.0mL of sterile NaHCO3 solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Vibrio vallismortis.

Vibrio vulnificus Agar (VVA) (BAM M190) Composition per liter: NaCl............................................................................................ 30.0g Agar ............................................................................................ 25.0g Peptone ....................................................................................... 20.0g Cellobiose solution ................................................................100.0mL Dye solution.............................................................................10.0mL pH 8.2 ± 0.2 at 25°C

Composition per liter:

Dye Solution: Composition per 100.0mL:

NaCl ............................................................................................ 30.0g Agar ............................................................................................ 15.0g

Bromthymol Blue ......................................................................... 0.6g Ethanol, 70%..........................................................................100.0mL

1886

Violet Peptone Bile Lactose Broth

Preparation of Dye Solution: Add Bromthymol Blue to 100.0mL of 70% ethanol. Mix thoroughly.

Cellobiose Solution: Composition per 100.0mL: Cellobiose ................................................................................... 10.0g

Preparation of Cellobiose Solution: Add cellobiose to distilled/ deionized water and bring volume to 100.0mL. Mix thoroughly. Gently heat while mixing to dissolve the cellobiose. Cool. Filter sterilize. Preparation of Medium: Add components, except cellobiose solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat until dissolved. Adjust pH to 8.2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 100.0mL sterile cellobiose solution. Mix thoroughly and pour into sterile Petri dishes. Final color of medium should be light blue.

Use: For the detection of Vibrio vulnificus from seafoods.

Violet Peptone Bile Lactose Broth Composition per liter: Lactose ........................................................................................ 10.0g Peptone........................................................................................ 10.0g Bile salts........................................................................................ 5.0g Gentian Violet ............................................................................. 0.04g pH 7.6 ± 0.2 at 25°C

Use: For the selective cultivation of members of the Enterobacteriaceae.

Pancreatic digest of gelatin........................................................... 7.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 3.0g Bile salts........................................................................................ 1.5g Neutral Red................................................................................. 0.03g Crystal Violet ............................................................................. 2.0mg pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems and Oxoid Unipath.

Use: For the detection of coliform bacteria in water and food.

Violet Red Bile Agar, HiVeg Composition per liter: Agar ............................................................................................ 15.0g Lactose........................................................................................ 10.0g Plant peptone ................................................................................ 7.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 3.0g Synthetic detergent No. I .............................................................. 1.5g Neutral Red................................................................................. 0.03g Crystal Violet ............................................................................. 2.0mg pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Preparation of Medium: Add components to distilled/deionized

Violet Red Bile Agar Composition per liter: Agar ............................................................................................ 15.0g Lactose ........................................................................................ 10.0g Glucose ....................................................................................... 10.0g Pancreatic digest of gelatin ........................................................... 7.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 3.0g Bile salts........................................................................................ 1.5g Neutral Red ................................................................................. 0.03g Crystal Violet ............................................................................. 2.0mg pH 7.4 ± 0.2 at 25°C

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Boil to dissolve components completely. Do not autoclave. Cool to 45°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the detection of coliform bacteria in water and food.

Violet Red Bile Agar with MUG Composition per liter:

water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour immediately into sterile Petri dishes or leave in tubes.

Agar ............................................................................................ 15.0g Lactose........................................................................................ 10.0g Pancreatic digest of gelatin........................................................... 7.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 3.0g Bile salts........................................................................................ 1.5g MUG (4-methylumbelliferyl-β-D-glucuronide)............................ 0.1g Neutral Red................................................................................. 0.03g Crystal Violet ............................................................................. 2.0mg pH 7.4 ± 0.2 at 25°C

Use: For the isolation and cultivation of members of the Enterobacteri-

Source: This medium is available as a premixed powder from BD Di-

Preparation of Medium: Add components to distilled/deionized

aceae from brined vegetables. For the enumeration of members of the Enterobacteriaceae from brined vegetables by the pour plate technique.

Violet Red Bile Agar (VRB Agar) Composition per liter: Agar ............................................................................................ 15.0g Lactose ........................................................................................ 10.0g © 2010 by Taylor and Francis Group, LLC

agnostic Systems.

Use: For the differentiation of Escherichia coli from dairy products and other foods based on their ability to produce β-glucuronidase.

Violet Red HiVeg Broth

Violet Red Bile Glucose Agar Composition per liter: Agar ............................................................................................ 12.0g Glucose ....................................................................................... 10.0g Peptone.......................................................................................... 7.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 3.0g Bile salts No. 3.............................................................................. 1.5g Neutral Red ................................................................................. 0.03g Crystal Violet ............................................................................. 2.0mg pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Do not autoclave. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the detection and enumeration of Enterobacteriaceae from foods.

Violet Red Glucose HiVeg Agar with Lactose Composition per liter: Agar ............................................................................................ 15.0g Lactose monohydrate .................................................................... 9.5g Glucose monohydrate ................................................................. 9.09g Plant peptone................................................................................. 7.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 3.0g Synthetic detergent No. I .............................................................. 1.5g Neutral Red ................................................................................. 0.03g Crystal Violet ............................................................................. 2.0mg pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Use: For the detection and enumeration of Enterobacteriaceae from raw foods.

Violet Red Glucose HiVeg Agar without Lactose Composition per liter: Agar ............................................................................................ 12.0g Glucose ....................................................................................... 10.0g Plant peptone................................................................................. 7.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 3.0g Synthetic detergent No. I .............................................................. 1.5g Neutral Red ................................................................................. 0.03g Crystal Violet ............................................................................. 2.0mg pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

1887

Use: For the detection and enumeration of Enterobacteriaceae from raw foods.

Violet Red HiVeg Agar Composition per liter: Agar ............................................................................................ 15.0g Lactose........................................................................................ 10.0g Plant peptone ................................................................................ 7.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 3.0g Synthetic detergent No. I .............................................................. 1.5g Neutral Red................................................................................. 0.03g Crystal Violet ............................................................................. 2.0mg pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Use: For the detection and enumeration of Enterobacteriaceae from foods. Recommended by the ISO Committee for selective isolation and enumeration of coli-aerogenes bacteria in water. For the detection and enumeration of coliforms from water and food.

Violet Red HiVeg Agar (1.2%) Composition per liter: Agar ............................................................................................ 12.0g Lactose........................................................................................ 10.0g Plant peptone ................................................................................ 7.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 3.0g Synthetic detergent No. I .............................................................. 1.5g Neutral Red................................................................................. 0.03g Crystal Violet ............................................................................. 2.0mg pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Violet Red HiVeg Broth Composition per liter: Plant peptone ................................................................................ 7.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 3.0g Lactose.......................................................................................... 1.5g Synthetic detergent No. I .............................................................. 1.5g Neutral Red................................................................................. 0.03g Crystal Violet ............................................................................. 2.0mg pH 7.4 ± 0.2 at 25°C

1888

Viral Transport Medium

Source: This medium is available as a premixed powder from HiMedia.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Do not autoclave.

Use: For the isolation and detection of coliforms from water, milk, and other foods.

Viral Transport Medium (VTM) Composition per 104.1mL: Bovine serum albumin .................................................................. 0.5g Veal infusion broth.................................................................100.0mL Phenol Red .................................................................................0.4mL Amphotericin B solution............................................................2.0mL Gentamicin solution ...................................................................1.0mL Vancomycin solution..................................................................0.2mL pH 7.4 ± 0.2 at 25°C

Veal Infusion Broth: Composition per liter: Veal, infusion from ................................................................... 500.0g NaCl .............................................................................................. 5.0g Pancreatic digest of casein ............................................................ 5.0g Peptic digest of animal tissue........................................................ 5.0g

Preparation of Veal Infusion Broth: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Use freshly prepared solution.

Vitamin B6 Blood Agar (ATCC Medium 860) Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein.......................................................... 15.0g Papaic digest of soybean meal...................................................... 5.0g NaCl.............................................................................................. 5.0g Sheep blood, defibrinated ........................................................50.0mL pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, except sheep blood, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile, defibrinated sheep blood. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation and maintenance of fastidious microorganisms, especially Streptococcus species.

Vitamin B6 Blood Agar with Pyridoxal-HCl (ATCC Medium 1511) Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of casein.......................................................... 15.0g Papaic digest of soybean meal...................................................... 5.0g NaCl.............................................................................................. 5.0g Pyridoxal·HCl ............................................................................. 0.01g Sheep blood, defibrinated ........................................................50.0mL pH 7.3 ± 0.2 at 25°C

Amphotericin B............................................................................. 2.5g

Preparation of Amphotericin B Solution: Add amphotericin B

Use: For the cultivation and maintenance of fastidious microorganisms,

Amphotericin B Solution: Composition per 10.0mL:

to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Gentamicin Solution: Composition per 10.0mL: Gentamicin.................................................................................... 0.5g

Preparation of Gentamicin Solution: Add gentamicin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Vancomycin Solution: Composition per 10.0mL: Vancomycin .................................................................................. 0.5g

Preparation of Vancomycin Solution: Add vancomycin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: To 100.0mL of sterile veal infusion broth, aseptically add bovine serum albumin, Phenol Red, amphotericin B solution, gentamicin solution, and vancomycin solution. Mix thoroughly. Dispense 2.0mL of medium into serum vials. Store at 4°C and use for up to 2 months.

especially Streptococcus species.

Vitamin B12 Assay Medium

Composition per liter:

Glucose ....................................................................................... 40.0g Sodium acetate............................................................................ 20.0g Vitamin assay casamino acids .................................................... 12.0g Sorbitan monooleate complex ...................................................... 2.0g K2HPO4......................................................................................... 1.0g KH2PO4......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.4g DL-Tryptophan............................................................................... 0.2g L-Cystine ....................................................................................... 0.2g Adenine....................................................................................... 0.02g FeSO4 .......................................................................................... 0.02g Guanine....................................................................................... 0.02g MnSO4·5H2O .............................................................................. 0.02g NaCl............................................................................................ 0.02g Uracil .......................................................................................... 0.02g Pyridoxine·HCl .......................................................................... 4.0mg Niacin......................................................................................... 2.0mg Riboflavin .................................................................................. 2.0mg Thiamine·HCl ............................................................................ 2.0mg Xanthine..................................................................................... 1.0mg

Vitamin B12 Medium Calcium pantothenate ................................................................200μg p-Aminobenzoic acid.................................................................200μg Folic acid....................................................................................100μg Biotin ...........................................................................................10μg pH 6.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Continue boiling for 2–3 min. Distribute into tubes in 5.0mL volumes. Add standard solution or test solutions to each tube. Adjust the volume of each tube to 10.0mL with distilled/deionized water. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the microbiological assaying of vitamin B12 using Lactobacillus leichmannii as the test organism.

Vitamin B12 Assay Medium with Colistin

Composition per liter:

Glucose ....................................................................................... 40.0g Sodium acetate ............................................................................ 20.0g Vitamin assay casamino acids..................................................... 12.0g Sorbitan monooleate complex ...................................................... 2.0g K2HPO4 ......................................................................................... 1.0g KH2PO4 ......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.4g Colistin sulfate .............................................................................. 0.5g DL-Tryptophan............................................................................... 0.2g L-Cystine ....................................................................................... 0.2g Adenine ....................................................................................... 0.02g FeSO4 .......................................................................................... 0.02g Guanine ....................................................................................... 0.02g MnSO4·5H2O .............................................................................. 0.02g NaCl ............................................................................................ 0.02g Uracil .......................................................................................... 0.02g Pyridoxine·HCl .......................................................................... 4.0mg Niacin......................................................................................... 2.0mg Riboflavin .................................................................................. 2.0mg Thiamine·HCl ............................................................................ 2.0mg Xanthine..................................................................................... 1.0mg Calcium DL-pantothenate...........................................................200μg p-Aminobenzoic acid.................................................................200μg Folic acid....................................................................................100μg Biotin ...........................................................................................10μg Cyanocobalamin ..................................................................... 250.0ng pH 6.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Continue boiling for 2–3 min. Distribute into tubes in 5.0mL volumes. Add standard solution or test solutions to each tube. Adjust the volume of each tube to 10.0mL with distilled/deionized water. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Lactobacillus leichmannii.

Vitamin B12 HiVeg Agar

Composition per liter:

Glucose ....................................................................................... 20.0g K2SO4 .......................................................................................... 20.0g Agar ............................................................................................ 15.0g © 2010 by Taylor and Francis Group, LLC

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Sodium acetate............................................................................ 12.0g Plant acid hydrolysate, vitamin free ........................................... 10.0g Soypeptone, vitamin free .............................................................. 5.0g Na-thioglycollate .......................................................................... 1.7g K2HPO4......................................................................................... 1.0g KH2PO4......................................................................................... 1.0g Polysorbate 80 .............................................................................. 1.0g Ribonucleic acid ........................................................................... 1.0g MgSO4 .......................................................................................... 0.4g L-Cystine....................................................................................... 0.2g DL-Tryptophan .............................................................................. 0.2g NaCl............................................................................................ 0.02g FeSO4 .......................................................................................... 0.02g MnSO4 ........................................................................................ 0.02g Adenine sulfate ....................................................................... 0.0176g Guanine hydrochloride ........................................................... 0.0124g Uracil .......................................................................................... 0.01g Xanthine (sodium) ...................................................................... 0.01g Pyridoxal-5-phosphate............................................................... 4.0mg Pyridoxine hydrochloride .......................................................... 4.0mg Calcium pantothenate ................................................................ 2.0mg Niacin......................................................................................... 2.0mg Riboflavin .................................................................................. 2.0mg Thiamine hydrochloride............................................................. 2.0mg Folic acid ................................................................................... 1.0mg Biotin .......................................................................................... 1.0μg pH 6.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Continue boiling for 2–3 min. Distribute into tubes in 5.0mL volumes. Add standard solution or test solutions to each tube. Adjust the volume of each tube to 10.0mL with distilled/deionized water. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the microbiological assaying of vitamin B12 using Lactobacillus leichmannii as the test organism.

Vitamin B12 Medium See: B12 Medium

Vitamin B12 Medium

Composition per liter:

Agar ............................................................................................ 15.0g Casein hydrolysate........................................................................ 6.0g K2HPO4......................................................................................... 0.2g MgSO4·7H2O ................................................................................ 0.2g Asparagine .................................................................................. 0.15g Vitamin B12 ............................................................................... 40.0μg FeSO4·7H2O................................................................................ 0.1μg Glycerol .....................................................................................2.0mL pH 7.0 ± 0.2 at 25°C

Use: For the cultivation of Escherichia coli.

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Vitamin B12 Nutrient Agar

Composition per liter:

Agar ............................................................................................ 15.0g Peptone.......................................................................................... 5.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 2.0g Beef extract ................................................................................... 1.0g Vitamin B12 ................................................................................ 0.4mg

Use: For the cultivation of Escherichia coli.

Vitamin Medium for Microbacterium Composition per liter: Casamino acids ........................................................................... 10.0g Glucose ....................................................................................... 10.0g (NH4)2SO4 ..................................................................................... 5.0g KH2PO4 ......................................................................................... 5.0g K2HPO4 ......................................................................................... 5.0g MgSO4·7H2O ................................................................................ 0.5g Vitamin solution.........................................................................4.0mL pH 7.0 ± 0.2 at 25°C

Vitamin Solution: Composition per 100.0mL: Thiamine ..................................................................................... 0.05g Riboflavin ................................................................................... 0.05g Pyridoxine·HCl ........................................................................... 0.05g Calcium pantothenate ................................................................. 0.05g Nicotinic acid .............................................................................. 0.01g Biotin .......................................................................................... 0.01g Folic acid..................................................................................... 0.01g p-Aminobenzoic acid .................................................................. 0.01g

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except vitamin solution, to distilled/deionized water and bring volume to 996.0mL. Mix thoroughly. Autoclave for 10 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 4.0mL of sterile vitamin solution. Mix thoroughly. Distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Microbacterium species.

VL Agar with Blood Composition per liter: Agar ............................................................................................ 20.0g Tryptone ...................................................................................... 10.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g Beef extract ................................................................................... 2.0g Glucose ......................................................................................... 2.0g L-Cysteine·HCl.............................................................................. 0.3g Sheep blood or horse blood ...................................................100.0mL pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components, except sheep blood, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Adjust pH to 7.4. Gently heat and bring to boiling. Autoclave for 15 © 2010 by Taylor and Francis Group, LLC

min at 15 psi pressure–121°C. Cool to 50°–55°C. Warm sheep blood to 50°C. Aseptically add 100.0mL of sterile sheep blood or 100.0mL of sterile horse blood. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Bacterionema helcogenes, Bacteroides nodosus, Bacteroides pyogenes, Bacteroides salivosus, Bacteroides suis, Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, Campylobacter coli, Campylobacter concisus, Campylobacter fetus, Campylobacter hyointestinalis, Campylobacter jejuni, Campylobacter lari, Campylobacter mucosalis, Campylobacter species, Campylobacter sputorum, Capnocytophaga gingivalis, Capnocytophaga ochracea, Capnocytophaga sputigena, Clostridium colinum, Clostridium difficile, Clostridium species, Clostridium spiroforme, Falcivibrio grandis, Falcivibrio vaginalis, Fusobacterium simiae, Gardnerella vaginalis, Leptotrichia buccalis, Pectinatus frisingensis, Peptostreptococcus anaerobius, Peptostreptococcus asaccharolyticus, Peptostreptococcus indolicus, Peptostreptococcus magnus, Peptostreptococcus micros, Peptostreptococcus prevotii, Peptostreptococcus tetradius, Propionibacterium acnes, Propionibacterium avidum, Propionibacterium granulosum, Propionibacterium lymphophilum, and Tonsillophilus suis.

VL Medium Composition per liter: Pancreatic digest of casein.......................................................... 10.0g Agar .............................................................................................. 6.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g Meat extract .................................................................................. 2.0g Glucose ......................................................................................... 2.0g L-Cysteine·HCl·H2O ..................................................................... 0.3g Antibiotic solution ...................................................................10.0mL pH 7.4 ± 0.2 at 25°C

Antibiotic Solution: Composition per 10.0mL: Kanamycin.................................................................................... 0.1g Vancomycin ............................................................................... 7.5mg

Preparation of Antibiotic Solution: Add components to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except antibiotic solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile antibiotic solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the isolation and cultivation of Bacteroides species.

VM1 Medium

NaN3 ........................................................................................... 0.05g Ethyl Violet ................................................................................. 0.05g pH 7.4 ± 0.2 at 25°C

Caution: Sodium azide is toxic. Azides also react with metals and disposal must be highly diluted.

Use: For the isolation and cultivation of Fusobacterium species.

VM Medium Composition per liter: KOH.............................................................................................. 4.5g Beef extract ................................................................................... 3.0g DL-Malic acid................................................................................ 2.5g Agar .............................................................................................. 2.0g NaCl .............................................................................................. 1.0g Yeast extract.................................................................................. 1.0g KH2PO4 ......................................................................................... 0.6g NH4Cl ........................................................................................... 0.5g K2HPO4 ......................................................................................... 0.4g MgSO4·7H2O ................................................................................ 0.2g Ferric EDTA............................................................................. 66.0mg CaCl2 ........................................................................................ 20.0mg MnSO4·H2O ............................................................................. 10.0mg Na2MoO4·2H2O ......................................................................... 2.0mg Biotin ......................................................................................... 0.1mg pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 6.8. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of unidentified bacteria ATCC 51563 and ATCC 51564.

VM1 Medium (DSMZ Medium 890) Composition per liter: NaCl ............................................................................................ 20.0g MgCl2·6H2O................................................................................ 12.6g Na2SO4 ........................................................................................ 3.24g CaCl2·2H2O................................................................................. 2.38g KCl.............................................................................................. 0.56g Sulfur, powdered........................................................................... 0.5g NH4Cl ........................................................................................... 0.3g K2HPO4 ......................................................................................... 0.2g NaHCO3 ...................................................................................... 0.16g Trace elements solution ...........................................................10.0mL pH 7.0 ± 0.2 at 25°C

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FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Mix thoroughly. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0 using H2SO4. Fill 20.0mL medium into 100.0mL serum bottles and seal with a rubber stopper. Add atmosphere of 78% H2 + 20% CO2 + 2% O2 with an overpressure. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the autotrophic cultivation of Hydrogenothermus marinus.

VM1 Medium (DSMZ Medium 890) Composition per liter: NaCl............................................................................................ 20.0g MgCl2·6H2O ............................................................................... 12.6g Peptone ......................................................................................... 5.0g Na2SO4 ........................................................................................ 3.24g CaCl2·2H2O ................................................................................ 2.38g Starch ............................................................................................ 2.0g Yeast extract.................................................................................. 1.0g KCl.............................................................................................. 0.56g Sulfur, powdered........................................................................... 0.5g NH4Cl ........................................................................................... 0.3g K2HPO4......................................................................................... 0.2g NaHCO3 ...................................................................................... 0.16g Trace elements solution ...........................................................10.0mL pH 7.0 ± 0.2 at 25°C

Trace Elements Solution: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl.............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoSO4·7H2O............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5 with KOH. Add remaining components. Add distilled/deionized water to 1.0L. Mix thoroughly. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0 using H2SO4. Fill 20.0mL medium into 100.0mL serum bottles and seal

1892

VMGII Medium

with a rubber stopper. Add atmosphere of 80% N2 + 20% air. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the heterotrophic cultivation of Hydrogenothermus marinus.

VMGII Medium (Viability-Preserving Microbiostatic Medium) Composition per 1100.0mL: Solution 1 ...............................................................................900.0mL Solution 2 ...............................................................................100.0mL Salt stock solution..................................................................100.0mL

Solution 1: Composition per 900.0mL: Noble agar..................................................................................... 0.1g

Preparation of Solution 1: Add agar to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Cool to 45°–50°C.

Solution 2: Composition per 100.0mL: Charcoal, bacteriological ............................................................ 10.0g Gelatin peptone ........................................................................... 10.0g Meat peptone................................................................................. 1.0g Cysteine·HCl................................................................................. 0.5g Thioglycolic acid .......................................................................0.5mL

Preparation of Biotin Solution: Add biotin to 50% ethanol and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Store at 5°C.

Preparation of Medium: Add components, except biotin solution, to distilled/deionized water and bring volume to 999.9mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 0.1mL of sterile biotin solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Neurospora species.

Vogel and Johnson Agar Composition per liter: Agar ............................................................................................ 16.0g Pancreatic digest of casein.......................................................... 10.0g D-Mannitol .................................................................................. 10.0g Glycine........................................................................................ 10.0g Yeast extract.................................................................................. 5.0g K2HPO4......................................................................................... 5.0g LiCl ............................................................................................... 5.0g Phenol Red................................................................................ 0.025g K2TeO3 solution.......................................................................20.0mL pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di-

Preparation of Solution 2: Add components to distilled/deionized

agnostic Systems and Oxoid Unipath.

water and bring volume to 100.0mL. Mix thoroughly.

Caution: Lithium chloride is harmful. Avoid bodily contact and inha-

Stock Salt Solution: Composition per liter:

lation of vapours. On contact with skin wash with plenty of water immediately.

Sodium glycerophosphate......................................................... 100.0g NaCl ............................................................................................ 10.0g KCl................................................................................................ 4.2g CaCl2·6H2O................................................................................... 2.4g MgSO4·7H2O ................................................................................ 1.0g Phenylmercuric acetate ............................................................... 0.03g

K2TeO3 Solution: Composition per 100.0mL:

Preparation of Stock Salt Solution: Add phenylmercuric acetate to approximately 800.0mL of distilled/deionized water. Gently heat. Add remaining components. Bring volume to 1.0L with distilled/deionized water.

K2TeO3 .......................................................................................... 1.0g

Preparation of K2TeO3 Solution: Add K2TeO3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Caution: Potassium tellurite is toxic.

100.0mL of solution 2 and 100.0mL of stock salt solution. Mix thoroughly. Distribute into screw-capped tubes. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except K2TeO3 solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 45°–50°C. Aseptically add 20.0mL of sterile K2TeO3 solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of oral streptococci, including

Use: For the detection of coagulase-positive Staphylococcus aureus.

Streptococcus mutans and Streptococcus sanguis, and nonspore-forming bacteria, including Lactobacillus species from human dental plaque.

Composition per liter:

Preparation of Medium: To 900.0mL of cooled solution 1, add

Vogel-Bonner Minimal Medium Composition per liter: K2HPO4 ...................................................................................... 10.0g NaNH4HPO4·H2O ........................................................................ 3.5g Citric acetate ................................................................................. 2.0g Glucose ......................................................................................... 2.0g MgSO4·7H2O......................................................................... 200.0mg Biotin solution............................................................................0.1mL

Vogel-Johnson Agar Base, HiVeg Agar ............................................................................................ 16.0g K2HPO4......................................................................................... 5.0g Glycine........................................................................................ 10.0g Plant hydrolysate ........................................................................ 10.0g Mannitol...................................................................................... 10.0g LiCl ............................................................................................... 5.0g Yeast extract.................................................................................. 5.0g Phenol Red................................................................................ 0.025g K2TeO3 solution.......................................................................20.0mL pH 7.2 ± 0.2 at 25°C

Source: This medium, without tellurite, is available as a premixed powder from HiMedia.

VP HiVeg Medium Caution: Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours. On contact with skin wash with plenty of water immediately.

K2TeO3 Solution: Composition per 100.0mL: K2TeO3 .......................................................................................... 1.0g

Preparation of K2TeO3 Solution: Add K2TeO3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Caution: Potassium tellurite is toxic. Preparation of Medium: Add components, except K2TeO3 solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 45°–50°C. Aseptically add 20.0mL of sterile K2TeO3 solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the detection of coagulase-positive Staphylococcus aureus. Vogel N Medium See: N DeVogel Medium

Vogel S Medium for Slime-Like Neurospora Yeast extract................................................................................ 0.75g Pancreatic digest of gelatin ........................................................... 0.5g Beef extract ................................................................................... 0.3g Vogel N 10X solution...............................................................10.0mL Sorbitol.......................................................................................7.5mL

Vogel N 10X Solution: Composition per 100.0mL: Sucrose........................................................................................ 15.0g KH2PO4 ........................................................................................ 5.0g Trisodium phosphate..................................................................... 3.0g MgSO4·7H2O................................................................................ 0.2g CaCl2·H2O solution..................................................................20.0mL Biotin solution............................................................................5.0mL Trace elements solution .............................................................5.0mL

Preparation of Vogel N Solution: Add components, except biotin solution and trace elements solution, to distilled/deionized water and bring volume to 70.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 5.0 mL of sterile biotin solution and 5.0mL of sterile trace elements solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

CaCl2·H2O Solution: Composition per 20.0mL: CaCl2·H2O .................................................................................... 0.1g

Preparation of CaCl2·H2O Solution: Add CaCl2·H2O to dis-

tilled/deionized water and bring volume to 20.0mL. Mix thoroughly.

Biotin Solution: Composition per 100.0mL: Biotin ......................................................................................... 5.0mg

Preparation of Biotin Solution: Add biotin to 50% ethanol and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Store at 5°C. Trace Elements Solution: Composition per 100.0mL: Citric acid·H2O ............................................................................. 5.0g ZnSO4·7H2O ................................................................................. 5.0g © 2010 by Taylor and Francis Group, LLC

1893

Fe(NH4)2(SO4)2·6H2O ................................................................. 1.0g CuSO4·5H2O............................................................................... 0.25g H3BO3, anhydrous ...................................................................... 0.05g MnSO4·H2O................................................................................ 0.05g Na2MoO4·2H2O.......................................................................... 0.05g

Preparation of Trace Elements Solutions: Add components successively to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly after addition of each component. Filter sterilize. Add 2–3mL of chloroform as a preservative. Store at 25°C. Preparation of Medium: Add components, except Vogel N 10X solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0mL of sterile Vogel N 10X solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Neurospora species. Voges-Proskauer Medium See: VP Medium

Von Hofsten & Malmquist Medium B Composition per liter: Agar ............................................................................................ 15.0g NaNO3 .......................................................................................... 2.0g K2HPO4......................................................................................... 0.5g CaCl2·H2O .................................................................................. 0.02g FeSO4·7H2O................................................................................ 0.02g MgSO4·7H2O .............................................................................. 0.02g MnSO4·H2O ................................................................................ 0.02g pH 7.5 ± 0.2 at 25°C

Use: For the cultivation of Alteromonas species and Cytophaga saccharophila. VP Agar See: Vibrio parahaemolyticus Agar

VP Broth, Modified, Smith, Gordon, and Clark Composition per liter: Proteose peptone........................................................................... 7.0g Glucose ......................................................................................... 5.0g NaCl.............................................................................................. 5.0g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes in 5.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of Bacillus cereus from foods.

VP HiVeg Medium Composition per liter: Agar ............................................................................................ 20.0g NaCl............................................................................................ 20.0g Sucrose........................................................................................ 20.0g Plant peptone .............................................................................. 10.0g Sodium citrate............................................................................. 10.0g Na2S2O3 ...................................................................................... 10.0g

1894

VP Medium

Synthetic detergent No. V............................................................. 5.0g Yeast extract.................................................................................. 5.0g Sodium lauryl sulfate .................................................................... 0.2g Bromthymol Blue ....................................................................... 0.04g Thymol Blue ............................................................................... 0.04g pH 6.9 ± 0.2 at 25°C

nies show a color change to red of the pH indicator. E. coli colonies show a fluorescence under UV light. Lactose-negative Enterobacteriaceae are colorless. Lactose-positive colonies are red and often surrounded by a turbid zone due to the precipitation of bile acids. Light blue fluorescing colonies denote E. coli.

Source: This medium is available as a premixed powder from Hi-

VRB MUG Agar (Violet Red Bile Lactose MUG Agar)

Media.

Preparation of Medium: Add components to distilled/deionized

Composition per liter:

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.9. Distribute into tubes in 3.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.

Agar ............................................................................................ 13.0g Lactose........................................................................................ 10.0g Meat peptone ................................................................................ 7.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 3.0g Bile salts mixture .......................................................................... 1.5g 4-Methylumbelliferyl-β-D-glucuronide ........................................ 0.1g Neutral Red................................................................................. 0.03g Crystal Violet ............................................................................ 0.002g pH 7.4 ± 0.2 at 37°C

Use: For the cultivation and differentiation of bacteria based on their ability to produce acetoin.

VP Medium (Voges-Proskauer Medium) Composition per liter: Peptone.......................................................................................... 7.0g K2HPO4 ......................................................................................... 5.0g Glucose ......................................................................................... 5.0g pH 6.9 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.9. Distribute into tubes in 3.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and differentiation of bacteria based on their ability to produce acetoin.

VPSA See: Vibrio parahaemolyticus Sucrose Agar VRB Agar See: Violet Red Bile Agar

VRB Agar, Fluorocult (Fluorocult VRB Agar) Composition per liter: Agar ............................................................................................ 13.0g Lactose ........................................................................................ 10.0g Peptone from meat ........................................................................ 7.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 3.0g Bile salts mixture .......................................................................... 1.5g 4-Methylumbelliferyl-β-D-glucuronide ........................................ 0.1g Neutral Red ................................................................................. 0.03g Crystal Violet ............................................................................ 0.002g pH 7.4 ± 0.2 at 25°C

Source: This medium is available from Merck. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Heat in a boiling water bath or in free flowing steam with frequent stirring until completely dissolved. Do not boil for more than 2 min. Do not autoclave. Do not overheat. Pour into sterile Petri dishes. The plates are clear and dark red.

Use: For the detection and enumeration of coliform bacteria, in particular E. coli. Crystal Violet and bile salts largely inhibit the growth of Gram-positive accompanying bacterial flora. Lactose-positive colo© 2010 by Taylor and Francis Group, LLC

Use: For the detection and enumeration of coliform bacteria, in particular E. coli. Gram-positive accompanying flora are extensively inhibited by Crystal Violet and bile salts. A color change to red indicates lactose-positive colonies, within which E. coli can be demonstrated by fluorescence in the UV.

VRE Agar Composition per 1004.0mL: Tryptone...................................................................................... 20.0g Agar ............................................................................................ 10.0g Yeast extract.................................................................................. 5.0g NaCl.............................................................................................. 5.0g Sodium citrate............................................................................... 1.0g Esculin .......................................................................................... 1.0g Ferric ammonium citrate............................................................... 0.5g NaN3 ........................................................................................... 0.15g Selective supplement solution ...................................................4.0mL pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath.

Selective Supplement Solution: Composition per 4.0mL: Meropenum................................................................................ 1.0mg Vancomycin ............................................................................... 6.0mg

Preparation of Selective Supplement Solution: Add components to distilled/deionized water and bring volume to 4.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except selective supplement solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptially add 4.0mL selective supplement solution. Mix thoroughly . Pour into sterile Petri dishes.

Vulcanibacillus Medium Use: For the isolation of vancomycin resistant enterococci (VRE) from clinical samples. Nonresistant enterococci containing the Van C genes will not grow on this medium.The selective supplement suppresses growth of Gram-negative bacteria and E. gallinarum. The medium contains an indicator system to detect the growth of esculinhydrolyzing organisms. Enterococci produce black zones around the colonies from the formation of black iron phenolic compounds derived from esculin-hydrolyis products and ferrous iron.

VRE Agar Composition per 1004.0mL: Tryptone ...................................................................................... 20.0g Agar ............................................................................................ 10.0g Yeast extract.................................................................................. 5.0g NaCl .............................................................................................. 5.0g Sodium citrate ............................................................................... 1.0g Esculin .......................................................................................... 1.0g Ferric ammonium citrate............................................................... 0.5g NaN3 ........................................................................................... 0.15g Selective supplement solution ...................................................4.0mL pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid

1895

Selective Supplement Solution: Composition per 4.0mL: Meropenum................................................................................ 2.0mg

Preparation of Selective Supplement Solution: Add meropenum to distilled/deionized water and bring volume to 4.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except selective supplement solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptially add 4.0mL selective supplement solution. Mix thoroughly . Aseptically distribute into sterile tubes. Use: For the isolation of high-level aminoglycoside-resistant enterococci (HLARE) from clinical samples. Nonresistant enterococci will not grow on this medium. The selective supplement suppresses growth of Gram-negative bacteria and E. gallinarum.

VTM See: Viral Transport Medium

Vulcanibacillus Medium (DSMZ Medium 1042)

Unipath.

Composition per liter:

Selective Supplement Solution: Composition per 4.0mL:

Sea salts, Sigma .......................................................................... 30.0g NaNO3 .......................................................................................... 1.0g Na-pyruvate ................................................................................. 1.0g Resazurin .................................................................................. 0.5mg VoSO4·2H2O ............................................................................ 0.05mg Vitamin solution.......................................................................20.0mL Yeast extract solution...............................................................10.0mL Bicarbonate solution ................................................................10.0mL Iron sulfate solution .................................................................10.0mL Wolfe's mineral elixir.................................................................2.0mL pH 6.9 ± 0.2 at 25°C

Gentamicin............................................................................. 512.0mg

Preparation of Selective Supplement Solution: Add gentamicin to distilled/deionized water and bring volume to 4.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except selective supplement solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptially add 4.0mL selective supplement solution. Mix thoroughly . Pour into sterile Petri dishes. Use: For the isolation of high-level aminoglycoside-resistant enterococci (HLARE) from clinical samples. Nonresistant enterococci containing the Van C genes will not grow on this medium.The selective supplement suppresses growth of Gram-negative bacteria and E. gallinarum. The medium contains an indicator system to detect the growth of esculin-hydrolyzing organisms. Enterococci produce black zones around the colonies from the formation of black iron phenolic compounds derived from esculin hydrolyis products and ferrous iron.

VRE Broth Composition per 1004.0mL: Calf brain infusion solids ............................................................ 12.5g Proteose peptone ......................................................................... 10.0g Beef heart infusion solids ............................................................. 5.0g NaCl .............................................................................................. 5.0g Na2HPO4 ....................................................................................... 2.5g Glucose ......................................................................................... 2.0g Selective supplement solution ...................................................4.0mL pH 7.4 ± 0.2 at 25°C

Yeast Extract Solution: Composition per 10.0mL: Yeast extract ................................................................................. 0.5g

Preparation of Yeast Extract Solution: Add yeast extract to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Sparge with 100% N2. Vitamin Solution: Composition per liter: Pyridoxine-HCl........................................................................ 10.0mg Thiamine-HCl·2H2O.................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg D-Ca-pantothenate ..................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

1896

Vulcanibacillus Medium

Wolfe’s Mineral Elixir: Composition per liter: MgSO4·7H2O .............................................................................. 30.0g NaCl ............................................................................................ 10.0g MnSO4·2H2O ................................................................................ 5.0g (NH4)2NiSO4·6H2O ...................................................................... 2.8g CoCl2·6H2O .................................................................................. 1.8g ZnSO4·7H2O ................................................................................. 1.8g FeSO4·7H2O.................................................................................. 1.0g CaCl2·2H2O................................................................................... 1.0g KAl(SO4)2·12H2O....................................................................... 0.18g CuSO4·5H2O ................................................................................. 0.1g H3BO3 ........................................................................................... 0.1g Na2MoO4·2H2O ............................................................................ 0.1g Na2SeO4 ........................................................................................ 0.1g Na2WO4·2H2O .............................................................................. 0.1g

Preparation of Wolfe’s Mineral Elixir: Adjust pH of 1.0L of distilled/deionized water to 1.0 with dilute H2SO4. Add remaining components one at a time. Mix thoroughly to dissolve.

Bicarbonate Solution: Composition per 10.0mL: NaHCO3 ........................................................................................ 1.0g

Iron sulfate solution .................................................................10.0mL Wolfe's mineral elixir.................................................................2.0mL pH 6.9 ± 0.2 at 25°C

Yeast Extract Solution: Composition per 10.0mL: Yeast extract ................................................................................. 2.0g

Preparation of Yeast Extract Solution: Add yeast extract to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Sparge with 100% N2. Vitamin Solution: Composition per liter: Pyridoxine-HCl........................................................................ 10.0mg Thiamine-HCl·2H2O.................................................................. 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid............................................................................. 5.0mg D-Ca-pantothenate ..................................................................... 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Lipoic acid ................................................................................. 5.0mg Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with a gas mixture of 80% N2 + 20% CO2. Filter sterilize.

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Iron Sulfate Solution: Composition per 10.0mL:

Wolfe’s Mineral Elixir: Composition per liter:

FeSO4·7H2O.................................................................................. 0.1g

Preparation of Iron Sulfate Solution: Add FeSO4·7H2O to 0.1N sulfuric acid and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Preparation of Medium: Add components, except iron sulfate, bicarbonate, yeast extract, and vitamin solutions, to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Adjust pH to 7.0. Gently heat and bring to boiling. Boil for 1 min. Cool to room temperature while sparging with 100% N2. Dispense into culture vessels under an atmosphere of 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add iron sulfate, bicarbonate, yeast extract, and vitamin solutions. Mix thoroughly. Adjust pH to 6.8–7.0. Aseptically dispense into culture vessels. It may be necessary to add 10–20mg sodium dithionite per liter (e.g., from a 5% (w/v) solution, freshly prepared under N2 and filter-sterilized), if the medium is not completely reduced after inoculation. Use: For the cultivation of Vulcanibacillus modesticaldus.

Vulcanibacillus Medium (DSMZ Medium 1042) Composition per liter: Sea salts, Sigma .......................................................................... 30.0g NaNO3........................................................................................... 1.0g Na-pyruvate ................................................................................. 1.0g Resazurin .................................................................................. 0.5mg VoSO4·2H2O ............................................................................ 0.05mg Vitamin solution.......................................................................20.0mL Yeast extract solution ...............................................................10.0mL Bicarbonate solution ................................................................10.0mL © 2010 by Taylor and Francis Group, LLC

MgSO4·7H2O .............................................................................. 30.0g NaCl............................................................................................ 10.0g MnSO4·2H2O ................................................................................ 5.0g (NH4)2NiSO4·6H2O ...................................................................... 2.8g CoCl2·6H2O .................................................................................. 1.8g ZnSO4·7H2O ................................................................................. 1.8g FeSO4·7H2O.................................................................................. 1.0g CaCl2·2H2O .................................................................................. 1.0g KAl(SO4)2·12H2O....................................................................... 0.18g CuSO4·5H2O ................................................................................. 0.1g H3BO3 ........................................................................................... 0.1g Na2MoO4·2H2O ............................................................................ 0.1g Na2SeO4 ........................................................................................ 0.1g Na2WO4·2H2O .............................................................................. 0.1g

Preparation of Wolfe’s Mineral Elixir: Adjust pH of 1.0L of distilled/deionized water to 1.0 with dilute H2SO4. Add remaining components one at a time. Mix thoroughly to dissolve. Bicarbonate Solution: Composition per 10.0mL: NaHCO3 ........................................................................................ 1.0g

Preparation of Bicarbonate Solution: Add NaHCO3 to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with a gas mixture of 80% N2 + 20% CO2. Filter sterilize.

Iron Sulfate Solution: Composition per 10.0mL: FeSO4·7H2O.................................................................................. 0.1g

Preparation of Iron Sulfate Solution: Add FeSO4·7H2O to 0.1N

sulfuric acid and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Vulcanithermus Medium Preparation of Medium: Add components, except iron sulfate, bicarbonate, yeast extract, and vitamin solutions, to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Adjust pH to 7.0. Gently heat and bring to boiling. Boil for 1 min. Cool to room temperature while sparging with 100% N2. Dispense into culture vessels under an atmosphere of 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Aseptically add iron sulfate, bicarbonate, yeast extract, and vitamin solutions. Mix thoroughly. Adjust pH to 6.8–7.0. Aseptically dispense into culture vessels. It may be necessary to add 10–20mg sodium dithionite per liter (e.g., from a 5% (w/v) solution, freshly prepared under N2 and filter-sterilized), if the medium is not completely reduced after inoculation. Use: For the cultivation of Clostridiisalibacter paucivoran.

Vulcanithermus Medium (DSMZ Medium 977) Composition per liter: NaCl ........................................................................................... 25.0g NH4Cl ........................................................................................ 0.33g KCl ............................................................................................. 0.33g Calcium chloride solution ........................................................10.0mL Magnesium chloride solution...................................................10.0mL Potassium nitrate solution ........................................................10.0mL Tryptone solution .....................................................................10.0mL Sucrose solution .......................................................................10.0mL Yeast extract solution ...............................................................10.0mL PIPES solution .......................................................................... 3.6mL Vitamin solution.........................................................................1.0mL Trace elements solution .............................................................1.0mL pH 6.8 ± 0.2 at 25°C

Trace Elements Solution: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl .............................................................................................. 1.0g MnSO4·2H2O ................................................................................ 0.5g CoSO4·7H2O ............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g CaCl2·2H2O................................................................................... 0.1g FeSO4·7H2O.................................................................................. 0.1g NiCl2·6H2O ............................................................................... 0.025g KAl(SO4)2·12H2O....................................................................... 0.02g H3BO3 ......................................................................................... 0.01g Na2MoO4·4H2O .......................................................................... 0.01g CuSO4·5H2O ............................................................................... 0.01g Na2SeO3·5H2O........................................................................... 0.3mg

1897

Folic acid ................................................................................... 2.0mg Vitamin B12 ................................................................................ 0.1mg

Preparation of Vitamin Solution: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Filter sterilize. PIPES Solution: Composition per liter: PIPES (Piperazine-N,N'-bis[2-ethane-sulfonic acid]) ................. 3.62

Preparation of PIPES Solution: Add PIPES to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Yeast Extract Solution: Composition per 10.0mL: Yeast extract ................................................................................. 0.5g

Preparation of Sucrose Solution: Add sucrose to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Sparge with 100% N2. Tryptone Solution: Composition per 10.0mL: Tryptone....................................................................................... 1.0g

Preparation of Tryptone Solution: Add tryptone to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Sparge with 100% N2. Postassium Nitrate Solution: Composition per 10.0mL: KNO3 .......................................................................................... 0.33g

Preparation of Potassium Nitrate Solution: Add KNO3 to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Sparge with 100% N2. Magnesium Chloride Solution: Composition per 10.0mL: MgCl2·6H2O ............................................................................... 0.33g

Preparation of Magnesium Chloride Solution: Add0.33g of MgCl2·6H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Sparge with 100% N2. Calcium Chloride Solution: Composition per 10.0mL: CaCl2·2H2O ................................................................................ 0.33g

Preparation of Calcium Chloride Solution: Add CaCl2·2H2O

to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Sparge with 100% N2.

Preparation of Medium: Add components, except calcium chloride, magnesium chloride, potassium nitrate, tryptone, yeast extract, vitamin, and sucrose solutions, to distilled/deionized water and bring

1898

VY Agar

volume to 940.0mL. Mix thoroughly. Sparge with 100% N2. Adjust pH to 6.8. Dispense into vessels suitable for anaerobic growth (Hungate tubes or serum bottles) under an atmosphere of 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Add calcium chloride, magnesium chloride, potassium nitrate, tryptone, yeast extract, vitamin, and sucrose solutions. Mix thoroughly. Adjust pH to 6.8. Aseptically dispense into culture vessels.

Use: For the cultivation of Vulcanithermus mediatlanticus.

VY Agar Composition per liter: Agar ............................................................................................ 15.0g Baker’s yeast............................................................................... 10.0g CaCl2·2H2O................................................................................... 1.0g Cyanocobalamin ........................................................................ 5.0mg pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of myxobacteria.

VY2 Agar Composition per liter: Agar ............................................................................................ 15.0g Baker’s yeast................................................................................. 5.0g CaCl2·2H2O................................................................................... 1.0g Cyanocobalamin ........................................................................ 5.0mg pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Bacillus subtilis.

VY/4-SWS Agar (DSMZ Medium 958) Composition per 1001.0mL: NaCl............................................................................................ 20.0g Agar ............................................................................................ 15.0g Yeast cell paste (baker’s yeast, washed in deionized water) wet weight .................................................. 2.5g Seawater salts solution..................................................................1.0L Vitamin B12 solution ..................................................................1.0mL pH 7.5 ± 0.2 at 25°C Seawater Salts Solution: Composition per liter: MgSO4·7H2O ................................................................................ 8.0g CaCl2·2H2O .................................................................................. 1.0g KCl................................................................................................ 0.5g NaHCO3 ...................................................................................... 0.16g KBr ............................................................................................. 0.08g SrCl2·6H2O ................................................................................. 0.03g H3BO3 ......................................................................................... 0.02g Ferric citrate................................................................................ 0.01g di-Na-ß-glycerophosphate .......................................................... 0.01g Trace elements solution SL-4 ....................................................1.0mL

Preparation of Trace Elements Solution SL-4: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Use: For the cultivation and maintenance of Myxococcus amylo-

Trace Elements Solution SL-6: Composition per liter:

vorans.

VY5 Agar Composition per liter: Agar ............................................................................................ 15.0g Baker’s yeast................................................................................. 2.0g CaCl2·2H2O................................................................................... 1.0g Cyanocobalamin ........................................................................ 5.0mg pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

H3BO3 ........................................................................................... 0.3g CoCl2·6H2O .................................................................................. 0.2g ZnSO4·7H2O ................................................................................. 0.1g MnCl2·4H2O ............................................................................... 0.03g Na2MoO4·H2O ............................................................................ 0.03g NiCl2·6H2O ................................................................................. 0.02g CuCl2··2H2O................................................................................ 0.01g

Preparation of Trace Elements Solution SL-6: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3.4.

Preparation of Seawater Salts Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Use: For the cultivation and maintenance of myxobacteria.

Vitamin B12 Solution: Composition per 10.0mL:

VY Medium (Veal Yeast Extract Medium) Composition per liter: Veal, solids from infusion ........................................................... 10.0g Pancreatic digest of casein ............................................................ 5.0g Peptic digest of animal tissue........................................................ 5.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g © 2010 by Taylor and Francis Group, LLC

Cyanocobalamin ........................................................................ 5.0mg

Preparation of Vitamin B12 Solution: Add cyanocobalamine to

distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Preparation of Medium: Add NaCl, agar, and yeast cell paste to 1.0L seawater salts solution. Mix thoroughly. Adjust pH to 7.5 with 1M NaOH. Gently heat and bring to boiling. Autoclave for 20 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 1.0mL sterile vitamin

Waksman’s Glucose Agar

B12 solution. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Haliangium ochraceum, Haliangium tepidum, Plesiocystis pacifica, and Enhygromyxa salina (Thaxtera salina).

W Medium Composition per liter: Sulfur .......................................................................................... 10.0g KH2PO4 ......................................................................................... 3.0g MgSO4·7H2O ................................................................................ 0.5g CaCl2·2H2O................................................................................. 0.25g (NH4)2SO4 ..................................................................................... 0.2g FeSO4·7H2O............................................................................. 10.0mg pH 3.0 ± 0.2 at 25°C

Preparation of Sulfur: Sterilize by steaming at 100°C for 60 min on 3 consecutive days.

Preparation of Medium: Add components, except sulfur, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0g of sterile sulfur. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Thiobacillus thiooxidans. Wadowsky-Yee Medium See: BCYE Selective Agar with PAV Wadowsky and Yee Medium, Modified See: MWY Medium

Wagatsuma Agar Composition per 1050.0mL: NaCl ............................................................................................ 70.0g Agar ............................................................................................ 15.0g Mannitol...................................................................................... 10.0g Peptone........................................................................................ 10.0g K2HPO4 ........................................................................................ 5.0g Yeast extract.................................................................................. 3.0g Crystal Violet ............................................................................. 1.0mg Red blood cells.........................................................................50.0mL pH 8.0 ± 0.2 at 25°C

Red Blood Cells: Composition per 100.0mL: Blood, human or rabbit ..........................................................100.0mL

1899

Wagatsuma HiVeg Agar Base with Red Blood Cells Composition per 1050.0mL: NaCl............................................................................................ 70.0g Agar ............................................................................................ 15.0g Plant peptone .............................................................................. 10.0g Mannitol...................................................................................... 10.0g K2HPO4......................................................................................... 5.0g Yeast extract.................................................................................. 3.0g Crystal Violet ............................................................................. 1.0mg Red blood cells ........................................................................50.0mL pH 8.0 ± 0.2 at 25°C

Source: This medium, without red blood cells, is available as a premixed powder from HiMedia.

Red Blood Cells: Composition per 100.0mL: Blood, human or rabbit ..........................................................100.0mL

Preparation of Red Blood Cells: Mix freshly drawn human or rabbit blood with anticoagulant and an equal volume of sterile 0.85% saline solution. Centrifuge cells at 4000 × g at 4°C for 15 min. Pour off saline and wash two more times with sterile saline. After last wash, pour off saline and resuspend cells to their original volume. Preparation of Medium: Add components, except blood, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.0. Place in a steam bath for 30 min. Do not autoclave. Cool to 45°–50°C. Add 50.0mL of washed red blood cells. Mix thoroughly. Pour into sterile Petri dishes. Dry plates before using.

Use: For the cultivation and detection of thermostable hemolysin of Vibrio parahaemolyticus by the Kanagawa reaction.

Wakimoto Medium, Modified Composition per liter: Agar ............................................................................................ 15.0g Sucrose........................................................................................ 15.0g Peptone ......................................................................................... 5.0g Na2HPO4·12 H2O.......................................................................... 2.0g Ca(NO3)2·4H2O ............................................................................ 0.5g FeSO4·7H2O.................................................................................. 0.5g

Use: For the cultivation and maintenance of Corynebacterium species and Pseudomonas species.

Waksman’s Glucose Agar

Preparation of Red Blood Cells: Mix freshly drawn human or

Composition per liter:

rabbit blood with anticoagulant and an equal volume of sterile 0.85% saline solution. Centrifuge cells at 4000 × g at 4°C for 15 min. Pour off saline and wash two more times with sterile saline. After last wash, pour off saline and resuspend cells to their original volume.

Agar ............................................................................................ 12.5g Glucose ....................................................................................... 10.0g Peptone ......................................................................................... 5.0g Beef extract................................................................................... 5.0g NaCl.............................................................................................. 5.0g pH 7.4–7.6 at 25°C

Preparation of Medium: Add components, except blood, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.0. Place in a steam bath for 30 min. Do not autoclave. Cool to 45°–50°C. Add 50.0mL of washed red blood cells. Mix thoroughly. Pour into sterile Petri dishes. Dry plates before using.

Use: For the cultivation and maintenance of Streptomyces species.

1900

Waksman’s Sulfur Medium

Waksman’s Sulfur Medium Composition per liter: KH2PO4 ......................................................................................... 3.0g MgSO4·7H2O ................................................................................ 0.5g (NH4)2SO4 ..................................................................................... 0.2g CaCl2·2H2O................................................................................... 0.2g Fe2(SO4)3 ................................................................................... 0.1mg

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. It is not necessary to sterilize this medium. Distribute into sterile tubes or flasks.

Use: For the cultivation of sulfate-reducing microorganisms from soil.

Wall Defective Bacterial Medium

Wallerstein Laboratory Differential Agar See: WL Differential Agar Wallerstein Laboratory Differential Medium See: WL Differential Medium Wallerstein Laboratory Medium with Tomato Juice See: WL Medium with Tomato Juice Wallerstein Laboratory Nutrient Agar See: WL Nutrient Agar Wallerstein Laboratory Nutrient Broth See: WL Nutrient Broth

Composition per liter: Sucrose...................................................................................... 100.0g Papaic digest of soybean meal .................................................... 20.0g Agarose ....................................................................................... 10.0g Yeast extract................................................................................ 10.0g NaCl .............................................................................................. 5.0g MgSO4·7H2O ................................................................................ 2.5g Horse serum ...........................................................................200.0mL Cholesterol solution .................................................................10.0mL pH 7.8 ± 0.2 at 25°C

Walsby Medium Composition per liter:

Cholesterol .................................................................................. 0.04g Ethanol (95% solution) ............................................................10.0mL

MgSO4·7H2O ............................................................................ 0.075g K2HPO4..................................................................................... 0.039g Na2CO3 ....................................................................................... 0.02g CaCl2·2H2O .............................................................................. 0.018g H3BO3 ........................................................................................ 2.8mg MnSO4·4H2O ............................................................................. 2.0mg ZnSO4 ...................................................................................... 0.22mg MoO3 ....................................................................................... 0.18mg CuSO4·5H2O ............................................................................ 0.08mg Co(NO3)2·6H2O ....................................................................... 0.05mg Iron-EDTA solution ...................................................................1.0mL pH 8.5 ± 0.2 at 25°C

Preparation of Cholesterol Solution: Add cholesterol to 10.0mL of 95% ethanol. Mix thoroughly. Filter sterilize.

Iron-EDTA Solution: Composition per liter:

Preparation of Medium: Add components, except cholesterol so-

EDTA .......................................................................................... 12.7g FeSO4·7H2O................................................................................ 4.98g

Cholesterol Solution: Composition per 10.0mL:

lution and horse serum, to distilled/deionized water and bring volume to 790.0mL. Mix thoroughly. Adjust pH to 7.8. Add 10.0mL of cholesterol solution. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 200.0mL of sterile horse serum. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of cell wall-deficient bacteria, such as L forms, that depend on osmotic stabilization.

Wallenstein Medium Composition per 4.225L: Malachite Green.......................................................................... 0.75g Egg yolk emulsion ....................................................................3.125L Glycerol .................................................................................100.0mL pH 6.75 ± 0.2 at 25°C

Egg Yolk Emulsion: Composition: Chicken egg yolks............................................................................ 66 Whole chicken egg............................................................................. 6

Preparation of Egg Yolk Emulsion: Soak eggs with 1:100 dilu-

Preparation of Iron-EDTA Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of planktonic gas-vacuolate cyanobacteria.

Wang’s Semisolid HiVeg Medium with Blood Composition per liter: Plant hydrolysate ........................................................................ 10.0g Plant peptone .............................................................................. 10.0g NaCl.............................................................................................. 5.0g Agar .............................................................................................. 4.0g Yeast extract.................................................................................. 2.0g Glucose ......................................................................................... 1.0g NaHSO3 ........................................................................................ 0.1g Sheep blood, defibrinated ......................................................100.0mL pH 7.0 ± 0.2 at 25°C

tion of saturated mercuric chloride solution for 1 min. Crack eggs and separate yolks from whites. Mix egg yolks with 6 chicken eggs.

Source: This medium, without sheep blood, is available as a premixed

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes. Autoclave for 15 min at 15 psi pressure–121°C.

powder from HiMedia.

Weitzman, Silva-Hutner Agar

1901

50.0mL of sterile distilled/deionized water. Mix thoroughly. Aseptically distribute into sterile screw-capped tubes in 4.0mL volumes. Allow tubes to cool in an upright position.

CaCl2·2H2O .................................................................................. 0.1g Maltose solution.....................................................................100.0mL pH 6.7 ± 0.2 at 25°C

Use: For the cultivation, transport, and maintenance of Campylobacter species from foods.

Maltose Solution: Composition per 100.0mL: Maltose ....................................................................................... 10.0g

Wang’s Transport Storage Medium Composition per liter: Pancreatic digest of casein .......................................................... 10.0g Peptic digest of animal tissue...................................................... 10.0g NaCl .............................................................................................. 5.0g Agar .............................................................................................. 4.0g Yeast extract.................................................................................. 2.0g Glucose ......................................................................................... 1.0g NaHSO3 ........................................................................................ 0.1g Sheep blood, defibrinated ......................................................100.0mL pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components, except sheep blood, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile sheep blood. Sheep blood may be replaced by 50.0mL of horse blood and 50.0mL of sterile distilled/deionized water. Mix thoroughly. Aseptically distribute into sterile screw-capped tubes in 4.0mL volumes. Allow tubes to cool in an upright position.

Use: For the cultivation, transport, and maintenance of Campylobacter species from foods.

Water Agar Composition per liter: Agar ............................................................................................ 20.0g

Preparation of Medium: Add agar to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and observation of sporulation of some fungi.

Water Agar Composition per liter: Agar ............................................................................................ 15.0g CaCl2·2H2O................................................................................... 1.0g pH 7.2 ± 0.2 at 25°C

Use: For the cultivation of myxobacteria.

Waxy Maize Starch Medium Composition per liter: Agar ............................................................................................ 20.0g Waxy maize starch ........................................................................ 5.0g Pancreatic digest of casein ............................................................ 5.0g Yeast extract.................................................................................. 5.0g CoCl2·6H2O .................................................................................. 0.1g © 2010 by Taylor and Francis Group, LLC

Preparation of Maltose Solution: Add maltose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except maltose solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 6.7. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add maltose solution. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Bacillus species. Wayne Sulfatase Agar See: Arylsulfatase Agar

WCX Agar Composition per liter: Agar ............................................................................................ 15.0g CaCl2·2H2O .................................................................................. 1.0g Cycloheximide solution .........................................................100.0mL pH 7.2 ± 0.2 at 25°C

Cycloheximide Solution Composition per 100.0mL: Cycloheximide........................................................................... 2.5mg

Preparation of Cycloheximide Solution: Add cycloheximide to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Caution: Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation.

Preparation of Medium: Add components, except cycloheximide solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile cycloheximide solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation of myxobacteria.

Weitzman, Silva-Hutner Agar (WSH Agar) (ATCC Medium 2032) Composition per liter: Agar ............................................................................................ 20.0g Alphacel...................................................................................... 20.0g Pablum Baby Oatmeal ................................................................ 10.0g Hunt's Tomato Paste ................................................................... 10.0g KH2PO4......................................................................................... 1.5g MgSO4·7H2O ................................................................................ 1.0g NaNO3. ......................................................................................... 1.0g

1902

Wesley Broth

Use: For the cultivation and maintenance of Penicillium spp. and other fungi.

Antibiotic Solution: Composition per 10.0mL:

Composition per liter:

Rifampin ................................................................................... 0.025g Cefsulodin................................................................................ 6.25mg Polymyxin B sulfate .............................................................. 20,000U

Wesley Broth Tryptose ...................................................................................... 20.0g Bicine (N,N-bis-2-[hydroxyethyl]glycine) buffer ....................... 10.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 2.5g Agar .............................................................................................. 1.0g FeSO4 .......................................................................................... 0.25g Na2S2O5 ...................................................................................... 0.25g Sodium pyruvate ......................................................................... 0.25g Antibiotic solution ...................................................................10.0mL Alkaline hematin solution ........................................................6.25mL

Preparation of Antibiotic Solution: Add components to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Antibiotic Solution: Composition per 10.0mL:

Preparation of Medium: Add components, except antibiotic solu-

Rifampin ................................................................................... 0.025g Cefsulodin ................................................................................ 6.25mg Polymyxin B sulfate............................................................... 20,000U

Preparation of Antibiotic Solution: Add components to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Alkaline Hematin Solution: Composition per 10.0mL: Hemin........................................................................................ 0.032g NaOH (0.15N solution)............................................................10.0mL

Alkaline Hematin Solution: Composition per 10.0mL: Hemin ....................................................................................... 0.032g NaOH (0.15N solution)............................................................10.0mL

Preparation of Hemin Solution: Add hemin to 10.0mL of NaOH solution. Mix thoroughly. Autoclave for 30 min at 5 psi pressure–108°C. Cool to 25°C. tion and alkaline hematin solution, to distilled/deionized water and bring volume to 983.75mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°– 50°C. Aseptically add 10.0mL of sterile antibiotic solution and 6.25mL of sterile alkaline hematin solution. Mix thoroughly. Aseptically distribute into sterile tubes. Use medium immediately or store overnight at 4°C.

Use: For the enrichment of Campylobacter species from foods.

Wesley HiVeg Broth Base with Antibiotics and Hematin Composition per liter:

tion and alkaline hematin solution, to distilled/deionized water and bring volume to 983.75mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°– 50°C. Aseptically add 10.0mL of sterile antibiotic solution and 6.25mL of sterile alkaline hematin solution. Mix thoroughly. Aseptically distribute into sterile tubes. Use medium immediately or store overnight at 4°C.

Plant hydrolysate No. 1............................................................... 20.0g Bicine.......................................................................................... 10.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 2.5g Agar .............................................................................................. 1.0g FeSO4 .......................................................................................... 0.25g Na2S2O3 ...................................................................................... 0.25g Sodium pyruvate......................................................................... 0.25g Antibiotic solution ...................................................................10.0mL Alkaline hematin solution........................................................6.25mL pH 8.0 ± 0.2 at 25°C

Use: For the enrichment of Campylobacter species from foods.

Source: This medium, without antibiotic and alkaline hematin solu-

Preparation of Hemin Solution: Add hemin to 10.0mL of NaOH solution. Mix thoroughly. Autoclave for 30 min at 5 psi pressure–108°C. Cool to 25°C.

Preparation of Medium: Add components, except antibiotic solu-

Wesley Broth Base with Antibiotics and Hematin Composition per liter:

tions, is available as a premixed powder from HiMedia.

Antibiotic Solution: Composition per 10.0mL:

Tryptose ...................................................................................... 20.0g Bicine .......................................................................................... 10.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 2.5g Agar .............................................................................................. 1.0g FeSO4 .......................................................................................... 0.25g Na2S2O3 ...................................................................................... 0.25g Sodium pyruvate ......................................................................... 0.25g Antibiotic solution ...................................................................10.0mL Alkaline hematin solution ........................................................6.25mL pH 8.0 ± 0.2 at 25°C

Source: This medium, without antibiotic and alkaline hematin solu-

solution. Mix thoroughly. Autoclave for 30 min at 5 psi pressure–108°C. Cool to 25°C.

Preparation of Antibiotic Solution: Add components to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Alkaline Hematin Solution: Composition per 10.0mL: Hemin ....................................................................................... 0.032g NaOH (0.15N solution)............................................................10.0mL

Preparation of Hemin Solution: Add hemin to 10.0mL of NaOH

Wickerham Broth Preparation of Medium: Add components, except antibiotic solution and alkaline hematin solution, to distilled/deionized water and bring volume to 983.75mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°– 50°C. Aseptically add 10.0mL of sterile antibiotic solution and 6.25mL of sterile alkaline hematin solution. Mix thoroughly. Aseptically distribute into sterile tubes. Use medium immediately or store overnight at 4°C.

Use: For the enrichment of Campylobacter species from foods.

Wheat Peptone Agar Composition per 750.0mL: Agar ............................................................................................ 20.0g Peptone........................................................................................ 20.0g Yeast extract................................................................................ 10.0g Glycerol ...................................................................................... 10.0g Wheat grain extract ................................................................500.0mL pH 6.8–7.0 at 25°C

Wheat Grain Extract: Composition per 500.0mL: Whole wheat ............................................................................... 30.0g

Preparation of Wheat Grain Extract: Add whole wheat to tap water and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring to boiling. Continue boiling for 60 min. Filter through several thicknesses of cheesecloth padded with cotton.

Preparation of Medium: Add agar, peptone, yeast extract, and glycerol to whole grain extract filtrate. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or aseptically distribute into sterile tubes.

Use: For the cultivation and utilization of Septoria nodorum.

Whey Agar Composition per liter: Whey permeate ........................................................................... 50.0g Casein hydrolysate ...................................................................... 20.0g Agar ............................................................................................ 15.0g Yeast extract................................................................................ 10.0g Tween™ 80 ................................................................................1.0mL pH 5.6 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.6. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Lactobacillus species.

1903

clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Lactococcus species.

Whey Broth Composition per liter: Whey permeate ........................................................................... 50.0g Casein hydrolysate...................................................................... 20.0g Yeast extract................................................................................ 10.0g Tween™ 80................................................................................1.0mL pH 5.6 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.6. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Lactobacillus species.

Wickerham Broth Composition per 100.0mL: Carbohydrate............................................................................... 10.0g Yeast nitrogen base ................................................................100.0mL

Yeast Nitrogen Base, 10X: Composition per liter: Glucose ....................................................................................... 10.0g KH2PO4......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g NaCl.............................................................................................. 0.1g CaCl2·2H2O .................................................................................. 0.1g DL-Methionine............................................................................. 0.02g DL-Tryptophan............................................................................. 0.02g L-Histidine·HCl ........................................................................... 0.01g Inositol ....................................................................................... 2.0mg H3BO3 ........................................................................................ 0.5mg ZnSO4·7H2O .............................................................................. 0.4mg MnSO4·4H2O ............................................................................. 0.4mg Thiamine·HCl ............................................................................ 0.4mg Pyridoxine.................................................................................. 0.4mg Niacin......................................................................................... 0.4mg Calcium pantothenate ................................................................ 0.4mg p-Aminobenzoic acid................................................................. 0.2mg Riboflavin .................................................................................. 0.2mg FeCl3 .......................................................................................... 0.2mg Na2MoO4·4H2O ......................................................................... 0.2mg KI ............................................................................................... 0.1mg CuSO4·5H2O............................................................................ 0.04mg

1904

Wickerham Broth

Folic acid.....................................................................................2.0μg Biotin ..........................................................................................2.0μg pH 4.5 ± 0.2 at 25°C

Preparation of Yeast Nitrogen Base: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: To 100.0mL of yeast nitrogen base, add 10.0g of carbohydrate. Mix thoroughly. Filter sterilize. Aseptically distribute 0.5mL into tubes containing 4.5mL of sterile, distilled/deionized water.

Use: For the cultivation and differentiation of bacteria based on carbohydrate assimilation.

Wickerham Broth Composition per 100.0mL: KNO3 .......................................................................................... 0.78g Yeast carbon base...................................................................100.0mL pH 4.5 ± 0.2 at 25°C

Yeast Carbon Base: Composition per liter: Glucose ....................................................................................... 10.0g KH2PO4 ......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g NaCl .............................................................................................. 0.1g CaCl2·2H2O................................................................................... 0.1g DL-Methionine............................................................................. 0.02g DL-Tryptophan............................................................................. 0.02g L-Histidine·HCl ........................................................................... 0.01g Inositol ....................................................................................... 2.0mg H3BO3 ........................................................................................ 0.5mg ZnSO4·7H2O .............................................................................. 0.4mg MnSO4·4H2O ............................................................................. 0.4mg Thiamine·HCl ............................................................................ 0.4mg Pyridoxine .................................................................................. 0.4mg Niacin......................................................................................... 0.4mg Calcium pantothenate ................................................................ 0.4mg p-Aminobenzoic acid ................................................................. 0.2mg Riboflavin .................................................................................. 0.2mg FeCl3 .......................................................................................... 0.2mg Na2MoO4·4H2O ......................................................................... 0.2mg KI ............................................................................................... 0.1mg CuSO4·5H2O ............................................................................ 0.04mg Folic acid.....................................................................................2.0μg Biotin ..........................................................................................2.0μg

Preparation of Yeast Carbon Base: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: To 100.0mL of yeast carbon base, add 0.78g of KNO3 (or peptone). Mix thoroughly. Filter sterilize. Aseptically distribute 0.5mL into tubes containing 4.5mL of sterile distilled/ deionized water.

Use: For the cultivation and differentiation of bacteria based on nitrate assimilation.

Wickerham Broth with Raffinose Composition per 100.0mL: Raffinose ..................................................................................... 20.0g Yeast nitrogen base ................................................................100.0mL © 2010 by Taylor and Francis Group, LLC

Yeast Nitrogen Base, 10X: Composition per liter: Glucose ....................................................................................... 10.0g KH2PO4......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g NaCl.............................................................................................. 0.1g CaCl2·2H2O .................................................................................. 0.1g DL-Methionine............................................................................. 0.02g DL-Tryptophan............................................................................. 0.02g L-Histidine·HCl ........................................................................... 0.01g Inositol ....................................................................................... 2.0mg H3BO3 ........................................................................................ 0.5mg ZnSO4·7H2O .............................................................................. 0.4mg MnSO4·4H2O ............................................................................. 0.4mg Thiamine·HCl ............................................................................ 0.4mg Pyridoxine.................................................................................. 0.4mg Niacin......................................................................................... 0.4mg Calcium pantothenate ................................................................ 0.4mg p-Aminobenzoic acid................................................................. 0.2mg Riboflavin .................................................................................. 0.2mg FeCl3 .......................................................................................... 0.2mg Na2MoO4·4H2O ......................................................................... 0.2mg KI ............................................................................................... 0.1mg CuSO4·5H2O ............................................................................ 0.04mg Folic acid .................................................................................... 2.0μg Biotin .......................................................................................... 2.0μg pH 4.5 ± 0.2 at 25°C

Preparation of Yeast Nitrogen Base: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: To 100.0mL of yeast nitrogen base, add 20.0g of raffinose. Mix thoroughly. Filter sterilize. Aseptically distribute 0.5mL into tubes containing 4.5mL of sterile distilled/deionized water.

Use: For the cultivation and differentiation of bacteria based on carbohydrate assimilation.

Wickerham Carbon Base Broth See: Yeast Carbon Base, 10X

Wilbrinck Agar for Xanthomonas Composition per liter: Sucrose........................................................................................ 20.0g Agar ............................................................................................ 12.0g Peptone ......................................................................................... 5.0g K2HPO4 ........................................................................................ 0.5g MgSO4·7H2O.............................................................................. 0.25g pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Pseudomonas caryophylli, Xanthomonas albilineans, and Xanthomonas axonopodis.

Wilbrinck Agar for Xanthomonas albilineans Composition per liter: Agar ............................................................................................ 20.0g Sucrose........................................................................................ 10.0g Peptone ......................................................................................... 5.0g

Wilkins-Chalgren Anaerobe Agar with GN Supplement

K2HPO4 ......................................................................................... 0.5g MgSO4·7H2O .............................................................................. 0.25g Na2SO3, anhydrous ..................................................................... 0.05g pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Xanthomonas albilineans and other Xanthomonas species.

Wilkins-Chalgren Agar Composition per liter: Agar ............................................................................................ 15.0g Gelatin peptone ........................................................................... 10.0g Pancreatic digest of casein .......................................................... 10.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g Glucose ......................................................................................... 1.0g L-Arginine ..................................................................................... 1.0g Sodium pyruvate ........................................................................... 1.0g Hemin......................................................................................... 5.0mg Vitamin K1 (menadione) ............................................................ 0.5mg pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Add antibiotic to be assayed; varying concentrations of antibiotics are used. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of anaerobic bacteria. For standardized antimicrobic susceptibility testing to determine the minimum inhibitory concentrations of antimicrobics for anaerobic bacteria.

Wilkins-Chalgren Anaerobe Agar Composition per liter: Agar ............................................................................................ 10.0g Pancreatic digest of casein .......................................................... 10.0g Gelatin peptone ........................................................................... 10.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g Glucose ......................................................................................... 1.0g L-Arginine ..................................................................................... 1.0g Sodium pyruvate ........................................................................... 1.0g Hemin......................................................................................... 5.0mg Menadione ................................................................................. 0.5mg Defibrinated blood ...................................................................50.0mL Tween™ 80 ................................................................................1.0mL pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath.

1905

55°C. Aseptically add 50.0mL of defibrinated blood. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of nonsporulating anaerobes.

Wilkins-Chalgren Anaerobe Agar with Cysteine Composition per liter: Agar ............................................................................................ 10.0g Pancreatic digest of casein.......................................................... 10.0g Gelatin peptone........................................................................... 10.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g Glucose ......................................................................................... 1.0g L-Arginine ..................................................................................... 1.0g Sodium pyruvate........................................................................... 1.0g L-Cysteine·HCl ............................................................................. 0.3g Hemin ........................................................................................ 5.0mg Menadione ................................................................................. 0.5mg Defibrinated blood ...................................................................50.0mL Tween™ 80................................................................................1.0mL pH 7.1 ± 0.2 at 25°C

Preparation of Medium: Add components, except defibrinated blood, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°– 55°C. Aseptically add 50.0mL of defibrinated blood. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of Agromonas oligotrophica, Falcivibrio grandis, and Falcivibrio vaginalis.

Wilkins-Chalgren Anaerobe Agar with GN Supplement Composition per liter: Agar ............................................................................................ 10.0g Pancreatic digest of casein.......................................................... 10.0g Gelatin peptone........................................................................... 10.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g Glucose ......................................................................................... 1.0g L-Arginine ..................................................................................... 1.0g Sodium pyruvate........................................................................... 1.0g Hemin ........................................................................................ 5.0mg Menadione ................................................................................. 0.5mg Defibrinated blood ...................................................................50.0mL GN anaerobe selective supplement..........................................20.0mL pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath.

GN Anaerobe Selective Supplement Composition per 20.0mL: Nalidixic acid........................................................................... 10.0mg Hemin ........................................................................................ 5.0mg Sodium succinate ....................................................................... 2.5mg Vancomycin ............................................................................... 2.5mg Menadione ................................................................................. 0.5mg

Preparation of GN Anaerobe Selective Supplement: Add components to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize.

1906

Wilkins-Chalgren Anaerobe Agar with NS Supplement

Preparation of Medium: Add components, except defibrinated blood and GN anaerobe selective supplement, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 20.0mL of GN anaerobe selective supplement and 50.0mL of defibrinated blood. Bring volume to 1.0L with distilled/deionized water. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes. Use: For the selective isolation of Gram-negative anaerobes.

Wilkins-Chalgren Anaerobe Agar with NS Supplement Composition per liter: Agar ............................................................................................ 10.0g Pancreatic digest of casein .......................................................... 10.0g Gelatin peptone ........................................................................... 10.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g Glucose ......................................................................................... 1.0g L-Arginine ..................................................................................... 1.0g Sodium pyruvate ........................................................................... 1.0g Hemin......................................................................................... 5.0mg Menadione ................................................................................. 0.5mg Defibrinated blood ...................................................................50.0mL NS anaerobe selective supplement .........................................20.0 mL Tween™ 80 ................................................................................1.0mL pH 7.1 ± 0.2 at 25°C

Hemin ........................................................................................ 5.0mg Menadione ................................................................................. 0.5mg pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems and Oxoid Unipath. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and antimicrobial susceptibility (MIC) testing of anaerobic bacteria.

Wilkins-Chalgren Anaerobe Medium with Yeast Extract Composition per liter: Yeast extract................................................................................ 20.0g Pancreatic digest of casein.......................................................... 10.0g Gelatin peptone........................................................................... 10.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g Glucose ......................................................................................... 1.0g L-Arginine ..................................................................................... 1.0g Sodium pyruvate........................................................................... 1.0g Hemin ........................................................................................ 5.0mg Menadione ................................................................................. 0.5mg pH 7.1 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

Source: This medium is available as a premixed powder from Oxoid Unipath.

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

NS Anaerobe Selective Supplement: Composition per 20.0mL:

Use: For the cultivation of Selenomonas acidaminovorans.

Sodium pyruvate ........................................................................... 1.0g Nalidixic acid .............................................................................. 0.01g Hemin......................................................................................... 5.0mg Menadione ................................................................................. 0.5mg

Wilkins-Chalgren Anaerobic HiVeg Agar Base with Blood Composition per liter:

Preparation of Medium: Add components, except defibrinated blood and NS anaerobe selective supplement, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 20.0mL of NS anaerobe selective supplement and 50.0mL of defibrinated blood. Bring volume to 1.0L with distilled/deionized water. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Agar ............................................................................................ 10.0g Plant hydrolysate ........................................................................ 10.0g Plant peptone .............................................................................. 10.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g L-Arginine..................................................................................... 1.0g Glucose ......................................................................................... 1.0g Sodium pyruvate........................................................................... 1.0g Fe4(P2O7)3·H2O ......................................................................... 5.0mg Menadione ................................................................................. 0.5mg Defibrinated blood ...................................................................50.0mL pH 7.1 ± 0.2 at 25°C

Use: For the selective isolation of nonsporulating anaerobes.

Source: This medium, without blood, is available as a premixed pow-

Preparation of NS Anaerobe Selective Supplement: Add components to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize.

der from HiMedia.

Wilkins-Chalgren Anaerobe Broth (Anaerobe Broth, MIC) Composition per liter: Pancreatic digest of casein .......................................................... 10.0g Gelatin peptone ........................................................................... 10.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g Glucose ......................................................................................... 1.0g L-Arginine ..................................................................................... 1.0g Sodium pyruvate ........................................................................... 1.0g © 2010 by Taylor and Francis Group, LLC

Preparation of Medium: Add components, except defibrinated blood, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°– 55°C. Aseptically add 50.0mL of defibrinated blood. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of nonsporulating anaerobes. For the cultivation and maintenance of anaerobic bacteria. For standardized antimicrobic susceptibility testing to determine the minimum inhibitory concentrations of antimicrobics for anaerobic bacteria.

Wilson Blair Base

Wilkins-Chalgren Anaerobic HiVeg Agar Base with Blood and Nonspore Anaerobic Supplement Composition per liter: Agar ............................................................................................ 10.0g Plant hydrolysate......................................................................... 10.0g Plant peptone............................................................................... 10.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g L-Arginine ..................................................................................... 1.0g Glucose ......................................................................................... 1.0g Sodium pyruvate ........................................................................... 1.0g Fe4(P2O7)3·H2O.......................................................................... 5.0mg Menadione ................................................................................. 0.5mg Defibrinated blood ...................................................................50.0mL Nonspore anaerobic supplement .......................................................... pH 7.1 ± 0.2 at 25°C

Source: This medium, without blood or nonspore anaerobic supplement, is available as a premixed powder from HiMedia. Nonspore Anaerobic Supplement: Composition per 10.0mL:

1907

flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°– 55°C. Aseptically add 50.0mL of defibrinated blood. Mix thoroughly. Aseptically distribute into tubes or leave in flasks.

Use: For the cultivation of nonsporulating anaerobes. For the cultivation and maintenance of anaerobic bacteria. For standardized antimicrobic susceptibility testing to determine the minimum inhibitory concentrations of antimicrobics for anaerobic bacteria.

Wilson and Blair’s Medium Composition per 165.0mL: Nutrient agar solution ............................................................100.0mL Solution B ................................................................................45.0mL Solution A................................................................................20.0mL pH 6.8 ± 0.2 at 25°C

Nutrient Agar Solution: Composition per 100.0mL: Agar ............................................................................................ 1.95g Pancreatic digest of gelatin......................................................... 0.65g Beef extract................................................................................. 0.39g

Sodium pyruvate ........................................................................... 1.0g Nalidixic acid ........................................................................... 10.0mg Ferric pyrophosphate, soluble.................................................... 5.0mg Menadione ................................................................................. 0.5mg

Preparation of Nutrient Agar Solution: Aseptically add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Preparation of Nonspore Anaerobic Supplement: Add com-

Solution A: Composition per liter:

ponents to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except defibrinated blood and nonspore anaerobic supplement, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 50.0mL of defibrinated blood and 10.0mL nonspore anaerobic supplement. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of nonsporulating anaerobes. For the cultivation and maintenance of anaerobic bacteria. For standardized antimicrobic susceptibility testing to determine the minimum inhibitory concentrations of antimicrobics for anaerobic bacteria.

Wilkins-Chalgren Anaerobic HiVeg Broth Base with Blood Composition per liter: Plant hydrolysate......................................................................... 10.0g Plant peptone............................................................................... 10.0g Yeast extract.................................................................................. 5.0g NaCl .............................................................................................. 5.0g Sodium pyruvate ........................................................................... 1.0g Glucose ......................................................................................... 1.0g L-Arginine ..................................................................................... 1.0g Fe4(P2O7)3·H2O.......................................................................... 5.0mg Menadione ................................................................................. 0.5mg Defibrinated blood ...................................................................50.0mL pH 7.1 ± 0.2 at 25°C

Source: This medium, without blood, is available as a premixed powder from HiMedia.

Na2HPO4 ................................................................................... 100.0g Na2SO3 ...................................................................................... 100.0g Glucose ....................................................................................... 50.0g Bismuth ammonium citrate......................................................... 30.0g

Preparation of Solution A: Aseptically add 30.0g of bismuth ammonium citrate to 250.0mL of boiling distilled/deionized water. Aseptically add Na2SO3 to 500.0mL of boiling distilled/deionized water. Mix the two solutions. Add the Na2HPO4 to the boiling mixture. Cool to 45°C. In a separate flask, aseptically add glucose to 250.0mL of sterile distilled/deionized water. Mix thoroughly. Aseptically add the glucose solution to the other cooled solution. Solution B: Composition per 225.0mL: Ferric citrate.................................................................................. 2.0g Brilliant Green ............................................................................ 0.55g

Preparation of Solution B: Aseptically add 2.0g of ferric citrate to 200.0mL of sterile distilled/deionized water. Mix thoroughly. Aseptically add Brilliant Green to 25.0mL of sterile distilled/deionized water. Mix thoroughly. Aseptically combine the two solutions. Preparation of Medium: Aseptically combine 100.0mL of nutrient agar solution, 20.0mL of solution A, and 45.0mL of solution B. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Clostridium welchii.

Wilson Blair Base Composition per liter: Agar ............................................................................................ 30.0g Proteose peptone No. 3 ............................................................... 10.0g Glucose ....................................................................................... 10.0g Beef extract................................................................................... 5.0g NaCl.............................................................................................. 5.0g

1908

Wilson Blair HiVeg Agar with Brilliant Green and Selective Reagent

Selective reagent ......................................................................70.0mL Brilliant Green (1% solution) ....................................................4.0mL pH 7.3 ± 0.2 at 25°C

Selective Reagent: Composition per 320.2mL: Solution 1 ...............................................................................100.0mL Solution 2 ...............................................................................100.0mL Solution 3 ...............................................................................100.0mL Solution 4 .................................................................................20.2mL

Solution 1: Composition per 100.0mL: NaHSO3 ...................................................................................... 40.0g

Preparation of Solution 1: Add NaHSO3 to 100.0mL of distilled/

deionized water. Mix thoroughly.

Solution 2: Composition per 100.0mL: NaH2PO4 ..................................................................................... 21.0g

Preparation of Solution 2: Add NaH2PO4 to 100.0mL of distilled/ deionized water. Mix thoroughly.

Solution 3: Composition per 100.0mL: Bismuth ammonium citrate......................................................... 12.5g

Preparation of Solution 3: Add bismuth ammonium citrate to 100.0mL of distilled/deionized water. Mix thoroughly.

Solution 4: Composition per 20.2mL: FeSO4 .......................................................................................... 0.96g

Preparation of Solution 4: Add FeSO4 to 20.0mL of distilled/de-

ionized water. Add 0.2mL of concentrated HCl. Mix thoroughly.

Preparation of Medium: Add components, except selective reagent and Brilliant Green solution, to distilled/deionized water and bring volume to 976.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add selective reagent and Brilliant Green solution. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation and cultivation of Salmonella, especially Salmonella typhi.

Wilson Blair HiVeg Agar with Brilliant Green and Selective Reagent Composition per liter: Agar ............................................................................................ 20.0g Plant peptone............................................................................... 10.0g Bismuth sulfite indicator............................................................... 8.0g Glucose ......................................................................................... 5.0g Plant extract .................................................................................. 5.0g Na2HPO4 ....................................................................................... 4.0g FeSO4 ............................................................................................ 0.3g Brilliant Green .......................................................................... 0.025g Selective reagent ......................................................................70.0mL pH 7.3 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

Source: This medium, without selective reagent, is available as a premixed powder from HiMedia.

Selective Reagent: Composition per 320.2mL: Solution 1...............................................................................100.0mL Solution 2...............................................................................100.0mL Solution 3...............................................................................100.0mL Solution 4.................................................................................20.2mL

Preparation of Selective Reagent: Combine 100.0mL of solution 1, 100.0mL of solution 2, 100.0mL of solution 3, and 20.2mL of solution 4. Mix thoroughly. Gently heat to boiling until a slate-grey color develops. Cool to 50°C. Solution 1: Composition per 100.0mL: NaHSO3 ...................................................................................... 40.0g

Preparation of Solution 1: Add NaHSO3 to 100.0mL of distilled/ deionized water. Mix thoroughly. Solution 2: Composition per 100.0mL: NaH2PO4 ..................................................................................... 21.0g

Preparation of Solution 2: Add NaH2PO4 to 100.0mL of distilled/ deionized water. Mix thoroughly.

Solution 3: Composition per 100.0mL: Bismuth ammonium citrate......................................................... 12.5g

Preparation of Solution 3: Add bismuth ammonium citrate to 100.0mL of distilled/deionized water. Mix thoroughly. Solution 4: Composition per 20.2mL: FeSO4 .......................................................................................... 0.96g

Preparation of Solution 4: Add FeSO4 to 20.0mL of distilled/deionized water. Add 0.2mL of concentrated HCl. Mix thoroughly. Preparation of Medium: Add components, except selective reagent and Brilliant Green solution, to distilled/deionized water and bring volume to 976.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add selective reagent and Brilliant Green solution. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation and cultivation of Salmonella, especially Salmonella typhi.

Wilson Blair HiVeg Agar Base with Selective Reagent and Brilliant Green Composition per liter: Agar ............................................................................................ 30.0g Glucose ....................................................................................... 10.0g Plant special peptone .................................................................. 10.0g Plant extract .................................................................................. 5.0g NaCl.............................................................................................. 5.0g Selective reagent......................................................................70.0mL Brilliant Green (1% solution) ....................................................4.0mL pH 7.3 ± 0.2 at 25°C

Source: This medium, without selective reagent or Brilliant Green, is available as a premixed powder from HiMedia.

Winogradsky’s N-Free Medium

1909

Winge Melibiose Agar

Selective Reagent: Composition per 100.0mL:

Composition per liter:

Solution 1 ...............................................................................100.0mL Solution 2 ...............................................................................100.0mL Solution 3 ...............................................................................100.0mL Solution 4.................................................................................20.2mL

Agar ............................................................................................ 15.0g Melibiose .................................................................................... 10.0g Yeast extract.................................................................................. 3.0g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

Solution 1: Composition per 100.0mL: NaHSO3 ...................................................................................... 40.0g

Preparation of Solution 2: Add NaH2PO4 to 100.0mL of distilled/ deionized water. Mix thoroughly.

Solution 3: Composition per 100.0mL: Bismuth ammonium citrate......................................................... 12.5g

Preparation of Solution 3: Add bismuth ammonium citrate to 100.0mL of distilled/deionized water. Mix thoroughly. Solution 4: Composition per 20.2mL:

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Saccharomyces cerevisiae.

Winogradsky’s Medium, Modified Composition per liter: CaCO3 ........................................................................................... 5.0g (NH4)2SO4 .................................................................................... 1.0g K2HPO4 ........................................................................................ 1.0g NaCl.............................................................................................. 1.0g MgSO4·7H2O ................................................................................ 0.5g FeSO4 ............................................................................................ 0.4g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until dissolved. Do not autoclave. Distribute into tubes or flasks. Swirl flask while dispensing to suspend precipitate.

Use: For the cultivation of nitrifying bacteria.

Winogradsky’s N-Free Medium

FeSO4 .......................................................................................... 0.96g

Composition per liter:

Preparation of Solution 4: Add FeSO4 to 20.0mL of distilled/deionized water. Add 0.2mL of concentrated HCl. Mix thoroughly.

Agar ............................................................................................ 20.0g CaCO3 ........................................................................................ 5.0mg Sugar solution ........................................................................100.0mL Concentrated salt solution..........................................................5.0mL pH 7.2 ± 0.2 at 25°C

Winge Agar Composition per liter: Glucose ....................................................................................... 20.0g Agar ............................................................................................ 15.0g Yeast extract.................................................................................. 3.0g pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Candida albicans, Candida kefyr, Candida tropicalis, Citeromyces matritensis, Cryptococcus albidus, Neurospora crassa, Octosporomyces octosporus, Neurospora sitophila, and Saccharomyces species. © 2010 by Taylor and Francis Group, LLC

Sugar Solution: Composition per 100.0mL: Sucrose or glucose ...................................................................... 10.0g

Preparation of Sugar Solution: Add sucrose or glucose to 100.0mL of distilled/deionized water. Mix thoroughly. Autoclave for 10 min at 10 psi pressure–115°C. Cool to 50°C.

Concentrated Salt Solution: Composition per liter: KH2PO4....................................................................................... 50.0g MgSO4·7H2O .............................................................................. 25.0g NaCl............................................................................................ 25.0g FeSO4·7H2O.................................................................................. 1.0g MnSO4·4H2O ................................................................................ 1.0g Na2MoO4·4H2O ............................................................................ 1.0g

Preparation of Concentrated Salt Solution: Add components to tap water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except sugar solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add sugar solution. Adjust pH to 7.2. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

1910

Winogradsky’s Nitrite Medium

Use: For the cultivation and maintenance of Azomonas insignis.

Source: This medium is available as a premixed powder from HiMedia.

Winogradsky’s Nitrite Medium

Caution: Cycloheximide is toxic. Avoid skin contact or aerosol for-

Composition per liter:

mation and inhalation.

Agar ............................................................................................ 15.0g NaNO2........................................................................................... 2.0g Na2CO3 , anhydrous ...................................................................... 1.0g K2HPO4 ......................................................................................... 0.5g

Preparation of Medium: Add components to distilled/deionized

Use: For the selective isolation and cultivation of Nocardia species and Rhodococcus species.

WL Differential Agar (Wallerstein Laboratory Differential Agar) Composition per liter: Glucose ....................................................................................... 50.0g Agar ............................................................................................ 20.0g Pancreatic digest of casein ............................................................ 5.0g Yeast extract.................................................................................. 4.0g KH2PO4 ....................................................................................... 0.55g KCl............................................................................................ 0.425g CaCl2·2H2O............................................................................... 0.125g MgSO4·7H2O ............................................................................ 0.125g Bromcresol Green ..................................................................... 0.022g Actidione® (cycloheximide) ...................................................... 4.0mg FeCl3 .......................................................................................... 2.5mg MnSO4·4H2O ............................................................................. 2.5mg pH 5.5 ± 0.2 at 25°C

Caution: Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation.

Use: For the differential cultivation of bacteria from industrial fermentation processes. Growth of yeasts and molds is inhibited.

WL Differential HiVeg Agar Composition per liter: Glucose ....................................................................................... 50.0g Agar ............................................................................................ 20.0g Plant hydrolysate........................................................................... 5.0g Yeast extract.................................................................................. 4.0g KH2PO4 ....................................................................................... 0.55g KCl............................................................................................ 0.425g MgSO4 ...................................................................................... 0.125g CaCl2 ......................................................................................... 0.125g Bromcresol Green ..................................................................... 0.022g Cycloheximide ........................................................................... 4.0mg FeCl3 .......................................................................................... 2.5mg MnSO4 ....................................................................................... 2.5mg pH 5.5 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

water and bring volume to 1.0L. Mix thoroughly. Gently heat with mixing and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the differential cultivation of bacteria from industrial fermentation processes. Growth of yeasts and molds is inhibited. For the selective isolation and enumeration of bacteria encountered in breweries and industrial fermentations.

WL Differential HiVeg Broth Composition per liter: Glucose ....................................................................................... 50.0g Plant hydrolysate .......................................................................... 5.0g Yeast extract.................................................................................. 4.0g KH2PO4....................................................................................... 0.55g KCl............................................................................................ 0.425g MgSO4 ...................................................................................... 0.125g CaCl2 ......................................................................................... 0.125g Bromcresol Green..................................................................... 0.022g Cycloheximide........................................................................... 4.0mg FeCl3 .......................................................................................... 2.5mg MnSO4 ....................................................................................... 2.5mg pH 5.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Caution: Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat with mixing and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

WL Differential Medium (Wallerstein Laboratory Differential Medium) Composition per liter: Glucose ....................................................................................... 50.0g Agar ............................................................................................ 20.0g Pancreatic digest of casein............................................................ 5.0g Yeast extract.................................................................................. 4.0g KH2PO4....................................................................................... 0.55g KCl............................................................................................ 0.425g CaCl2·2H2O .............................................................................. 0.125g MgSO4·7H2O ............................................................................ 0.125g Bromcresol Green..................................................................... 0.022g Actidione® (cycloheximide) ...................................................... 4.0mg FeCl3 .......................................................................................... 2.5mg MnSO4·4H2O ............................................................................. 2.5mg pH 5.5 ± 0.2 at 25°C

WL Nutrient Agar

1911

Source: This medium is available as a premixed powder from BD Di-

Preparation of Medium: Add components to distilled/deionized

agnostic Systems.

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Caution: Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation.

Use: For the cultivation of microorganisms from alcoholic mash.

Use: For the differential cultivation of bacteria from industrial fermentation processes. Growth of yeasts and molds is inhibited.

WL Differential Medium (Wallerstein Laboratory Differential Medium) Composition per liter: Glucose ....................................................................................... 50.0g Agar ............................................................................................ 20.0g Pancreatic digest of casein ............................................................ 5.0g Yeast extract.................................................................................. 4.0g KH2PO4 ....................................................................................... 0.55g KCl............................................................................................ 0.425g CaCl2·2H2O............................................................................... 0.125g MgSO4·7H2O ............................................................................ 0.125g Bromcresol Green ..................................................................... 0.022g Actidione® (cycloheximide) ...................................................... 4.0mg FeCl3 .......................................................................................... 2.5mg MnSO4·4H2O ............................................................................. 2.5mg Na2CO3 (1% solution) ............................................................30.0mL pH 6.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Caution: Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation.

Use: For the differential cultivation of bacteria from industrial fermentation processes. Growth of yeasts and molds is inhibited.

WL Medium with Tomato Juice (Wallerstein Laboratory Medium with Tomato Juice) Composition per liter: Glucose ....................................................................................... 50.0g Pancreatic digest of casein ............................................................ 5.0g Yeast extract.................................................................................. 4.0g KH2PO4 ....................................................................................... 0.55g KCl............................................................................................ 0.425g CaCl2 ......................................................................................... 0.125g MgSO4·7H2O ............................................................................ 0.125g Bromcresol Green ..................................................................... 0.022g FeCl3 .......................................................................................... 2.5mg MnSO4·4H2O ............................................................................. 2.5mg Tomato juice, canned, clarified..............................................400.0mL pH 5.5 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

WL Nutrient Agar (Wallerstein Laboratory Nutrient Agar) Composition per liter: Glucose ....................................................................................... 50.0g Agar ............................................................................................ 20.0g Pancreatic digest of casein............................................................ 5.0g Yeast extract.................................................................................. 4.0g KH2PO4....................................................................................... 0.55g KCl............................................................................................ 0.425g CaCl2·2H2O .............................................................................. 0.125g MgSO4·7H2O ............................................................................ 0.125g Bromcresol Green..................................................................... 0.022g FeCl3 .......................................................................................... 2.5mg MnSO4·4H2O ............................................................................. 2.5mg pH 5.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems and Oxoid Unipath.

Use: For the detection of bacteria and yeasts in industrial fermentation processes, particularly from beer processing.

Source: This medium is available as a premixed powder from BD Diagnostic Systems and Oxoid Unipath.

Use: For the detection of bacteria and yeasts from industrial fermentation processes, particularly from beer processing.

1912

WL Nutrient Broth

WL Nutrient Broth (Wallerstein Laboratory Nutrient Broth) Composition per liter: Glucose ....................................................................................... 50.0g Pancreatic digest of casein ............................................................ 5.0g Yeast extract.................................................................................. 4.0g KH2PO4 ....................................................................................... 0.55g KCl............................................................................................ 0.425g CaCl2 ......................................................................................... 0.125g MgSO4·7H2O ............................................................................ 0.125g Bromcresol Green ..................................................................... 0.022g FeCl3 .......................................................................................... 2.5mg MnSO4·4H2O ............................................................................. 2.5mg pH 5.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems and Oxoid Unipath.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of yeasts, molds, and bacteria found in brewing and other industrial fermentation processes.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the control of brewing and other fermentation processes.

WL Nutrient HiVeg Broth Composition per liter: Glucose ....................................................................................... 50.0g Plant hydrolysate .......................................................................... 5.0g Yeast extract.................................................................................. 4.0g KH2PO4....................................................................................... 0.55g KCl............................................................................................ 0.425g MgSO4 ...................................................................................... 0.125g CaCl2 ......................................................................................... 0.125g Bromcresol green...................................................................... 0.022g FeCl3 .......................................................................................... 2.5mg MnSO4 ....................................................................................... 2.5mg pH 5.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

WL Nutrient HiVeg Medium Composition per liter:

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Glucose ....................................................................................... 50.0g Agar ............................................................................................ 20.0g Plant hydrolysate .......................................................................... 5.0g Yeast extract.................................................................................. 4.0g KH2PO4....................................................................................... 0.55g KCl............................................................................................ 0.425g CaCl2 ......................................................................................... 0.125g MgSO4 ...................................................................................... 0.125g Bromcresol green...................................................................... 0.022g FeCl3 .......................................................................................... 2.5mg MnSO4 ....................................................................................... 2.5mg pH 5.5 ± 0.2 at 25°C

Use: For the cultivation of yeasts, molds, and bacteria found in brew-

Source: This medium is available as a premixed powder from Hi-

ing and other industrial fermentation processes.

Media.

Source: This medium is available as a premixed powder from BD Diagnostic Systems and Oxoid Unipath.

Preparation of Medium: Add components to distilled/deionized

WL Nutrient Broth (Wallerstein Laboratory Nutrient Broth) Composition per liter: Glucose ....................................................................................... 50.0g Pancreatic digest of casein ............................................................ 5.0g Yeast extract.................................................................................. 4.0g FeCl3 ............................................................................................. 2.5g MnSO4·4H2O ................................................................................ 2.5g KH2PO4 ....................................................................................... 0.55g KCl............................................................................................ 0.425g CaCl2·2H2O............................................................................... 0.125g MgSO4·7H2O ............................................................................ 0.125g Bromcresol Green ..................................................................... 0.022g pH 5.5 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

WMC Medium Composition per 1010.0mL: NaHCO3 ........................................................................................ 5.0g Sodium acetate.............................................................................. 1.0g L-Cysteine ..................................................................................... 0.5g

Wolinella succinogenes Medium

Resazurin ................................................................................... 1.0mg Mineral salt solution 2xW......................................................500.0mL LIP solution..............................................................................50.0mL TYC solution............................................................................50.0mL Trace elements solution ...........................................................10.0mL Na2S·9H2O solution .................................................................10.0mL pH 7.2 ± 0.2 at 25°C

Mineral Solution 2xW: Composition per liter: NaCl ............................................................................................ 40.0g MgCl2·6H2O.................................................................................. 5.6g MgSO4·7H2O ................................................................................ 0.7g KCl.............................................................................................. 0.68g NH4Cl ........................................................................................... 0.5g CaCl2·2H2O................................................................................. 0.28g K2HPO4 ...................................................................................... .0.28g

Preparation of Mineral Solution 2xW: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Store at room temperature in the dark.

LIP Solution: Composition per liter: L-Isoleucine................................................................................. 10.0g L-Leucine ...................................................................................... 5.0g Pantothenate.................................................................................. 0.1g

Preparation of LIP Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. May be stored unsterilized at −20°C.

TYC Solution: Composition per liter: Casamino acids ......................................................................... 100.0g Yeast extract................................................................................ 50.0g L-Tryptophan................................................................................. 1.0g

Preparation of TYC Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Trace Elements Solution: Composition per liter: MgSO4·7H2O ............................................................................... 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl .............................................................................................. 1.0g MnSO4·2H2O ............................................................................... 0.5g CoSO4·7H2O ............................................................................... 0.18g ZnSO4·7H2O ............................................................................... 0.18g FeSO4·7H2O.................................................................................. 0.1g CaCl2·2H2O................................................................................... 0.1g KAI(SO4)2·12H2O....................................................................... 0.02g CuSO4·5H2O ............................................................................... 0.01g H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O ......................................................................... 0.01g NiCl2·6H2O ............................................................................... 0.025g Na2SeO3·5H2O........................................................................... 0.3mg

1913

Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O.................................................................................... 0.5g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Preparation of Medium: Prepare and dispense medium under 80% H2 + 20% CO2. Add components, except TYC solution and Na2S·9H2O solution, to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Sparge with 80% H2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically add 50.0mL of sterile TYC solution and 10.0mL of sterile Na2S·9H2O solution. Mix thoroughly.

Use: For the cultivation and maintenance of Methanococcus voltae.

Wolin-Bevis Medium Composition per liter: Agar ............................................................................................ 20.0g (NH4)2SO4 .................................................................................... 1.0g KH2HPO4 ...................................................................................... 1.0g Glucose ....................................................................................... 0.25g L-Histidine·HCl ........................................................................... 0.25g Tween™ 80 (polysorbate 80) ....................................................3.0mL pH 5.4 ± 0.2 at 25°C

Use: For the enhanced production of chlamydospores by Candida albicans.

Wolinella succinogenes Medium Composition per liter: K2HPO4......................................................................................... 5.0g Fumaric acid ................................................................................. 3.0g Sodium formate ............................................................................ 3.0g (NH4)2SO4 .................................................................................... 1.0g Yeast extract.................................................................................. 1.0g MgCl2·6H2O ................................................................................. 0.2g FeSO4·7H2O................................................................................ 0.02g Resazurin ................................................................................... 1.0mg Sodium thioglycolate solution .................................................10.0mL pH 7.0 ± 0.2 at 25°C

Sodium Thioglycolate Solution: Composition per 10.0mL: Sodium thioglycolate .................................................................... 0.5g

Preparation of Sodium Thioglycolate Solution: Add sodium thioglycolate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Preparation of Medium: Add components, except sodium thioglycolate solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure– 121°C. Aseptically add 10.0mL of sterile sodium thioglycolate solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Wolinella succinogenes.

1914

Woods and Welton Agar

Woods and Welton Agar Composition per liter: NaCl ............................................................................................ 23.4g Casein hydrolysate ...................................................................... 17.0g Agar ............................................................................................ 15.0g Glycerol ...................................................................................... 10.0g Pancreatic digest of gelatin ........................................................... 5.0g Glucose ......................................................................................... 5.0g Papaic digest of soybean meal ...................................................... 3.0g Beef extract ................................................................................... 3.0g Yeast extract.................................................................................. 2.0g Casamino acids, vitamin free........................................................ 0.5g Pancreatic digest of casein ............................................................ 0.5g Na2SO3 .......................................................................................... 0.1g pH 7.6 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.6. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and enumeration of yeasts. The low pH of the agar selectively inhibits bacterial growth.

Wort Agar Composition per liter: Agar ............................................................................................ 25.0g Yeast extract.................................................................................. 1.0g Wort solution.................................................................................1.0L

Wort Solution: Composition per liter: Malt extract............................................................................... 110.0g

Preparation of Wort Solution: Add malt extract to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Preparation of Medium: Combine components. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and mainteneance of a wide variety of yeasts and filamentous fungi.

Use: For the cultivation of Vibrio alginolyticus.

Wort Agar 6°Brix Composition per liter:

Worfel-Ferguson Agar Composition per liter: Sucrose........................................................................................ 20.0g Agar ............................................................................................ 15.0g NaCl .............................................................................................. 2.0g Yeast extract.................................................................................. 2.0g K2SO4 ............................................................................................ 1.0g MgSO4·7H2O .............................................................................. 0.25g pH 6.5 ± 0.2 at 25°C

Use: For the detection of capsule production by Klebsiella. For serological detection of the Neufeld (Quellung) reaction.

Wort Agar Composition per liter: Agar ............................................................................................ 15.0g Malt extract ................................................................................. 15.0g Maltose...................................................................................... 12.75g Dextrin ........................................................................................ 2.75g Glycerol ...................................................................................... 2.35g K2HPO4 ......................................................................................... 1.0g NH4Cl ........................................................................................... 1.0g Pancreatic digest of gelatin ......................................................... 0.78g pH 4.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di-

Malt........................................................................................... 250.0g Agar ............................................................................................ 20.0g

Preparation of Medium: Add 250.0g of ground malt to tap water and bring volume to 1.0L. Mix thoroughly. Gently heat to 55°–60°C. Maintain at 55°–60°C for 1.5–2.0 hr while mixing regularly. Elevate temperature to 80°C and maintain for 10 min with thorough mixing. Cool the wort to 25°C and filter through a linen bag. Adjust the concentration of sugars to 6° Brix (specific gravity of 1.024 at 24°C). Add 20.0g of agar. Gently heat and bring to boiling. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 30 min at 3 psi pressure– 105°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Rhodococcus erythropolis.

Wort Broth Composition per liter: Yeast extract.................................................................................. 1.0g Wort solution.................................................................................1.0L

Wort Solution: Composition per liter: Malt extract............................................................................... 110.0g

Preparation of Wort Solution: Add malt extract to distilled/deionized water and bring volume to 1000.0mL. Mix thoroughly. Preparation of Medium: Combine components. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation of a wide variety of yeasts and filamentous fungi.

agnostic Systems and Oxoid Unipath.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Boil for 1 min with mixing. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Do not overheat, as this will result in hydrolysis of the agar. An additional 5.0g of agar can be used to make a firmer agar. Pour into sterile Petri dishes or leave in tubes. © 2010 by Taylor and Francis Group, LLC

Wort HiVeg Agar Composition per liter: Agar ............................................................................................ 15.0g Malt extract................................................................................. 15.0g Maltose ..................................................................................... 12.75g Dextrin ........................................................................................ 2.75g

Xanthobacter agilis Agar

NH4Cl ........................................................................................... 1.0g K2HPO4 ......................................................................................... 1.0g Plant peptone............................................................................... 0.78g pH 4.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

1915

Wort Sucrose Broth Composition per liter: Yeast extract.................................................................................. 1.0g Wort solution..........................................................................500.0mL Sucrose solution.....................................................................500.0mL

Wort Solution: Composition per 500.0mL: Malt extract................................................................................. 55.0g

Preparation of Wort Solution: Add malt extract to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Sucrose Solution: Composition per 500.0mL:

Use: For the cultivation and enumeration of yeasts. The low pH of the

Sucrose........................................................................................ 50.0g

agar selectively inhibits bacterial growth.

Preparation of Sucrose Solution: Add sucrose to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly.

Wort HiVeg Broth Composition per liter: Malt extract ................................................................................. 15.0g Maltose...................................................................................... 12.75g Dextrin ........................................................................................ 2.75g NH4Cl ........................................................................................... 1.0g K2HPO4 ......................................................................................... 1.0g Plant peptone............................................................................... 0.78g pH 4.8 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Preparation of Medium: Add components to distilled/deionized

Preparation of Medium: Combine components. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation of Aspergillus oryzae.

Xanthine Agar Composition per liter: Solution 1...............................................................................900.0mL Solution 2...............................................................................100.0mL pH 7.0 ± 0.2 at 25°C

Solution 1: Composition per 900.0mL:

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Boil for 1 min with mixing. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Agar ............................................................................................ 15.0g Pancreatic digest of gelatin........................................................... 5.0g Beef extract................................................................................... 3.0g

Use: For the cultivation and enumeration of yeasts. The low pH of the

Preparation of Solution 1: Add components to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling.

agar selectively inhibits bacterial growth.

Wort Sucrose Agar Composition per liter:

Solution 2: Composition per 100.0mL: Xanthine........................................................................................ 4.0g

Agar ............................................................................................ 25.0g Yeast extract.................................................................................. 1.0g Wort solution..........................................................................500.0mL Sucrose solution .....................................................................500.0mL

Preparation of Solution 2: Add xanthine to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Gently heat and bring to boiling.

Wort Solution: Composition per 500.0mL:

oughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Malt extract ................................................................................. 55.0g

Preparation of Wort Solution: Add malt extract to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly.

Sucrose Solution: Composition per 500.0mL: Sucrose........................................................................................ 50.0g

Preparation of Sucrose Solution: Add sucrose to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Preparation of Medium: Combine components. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or distribute into sterile tubes.

Preparation of Medium: Combine solutions 1 and 2. Mix thor-

Use: For the differentiation of aerobic Actinomycete species. Clearing around a colony indicates utilization of xanthine. Streptomyces species utilize xanthine; most Nocardia and Actinomadura species do not utilize xanthine.

Xanthobacter agilis Agar Composition per 1100.0mL: Solution A.....................................................................................1.0L Solution B ..............................................................................100.0mL

Solution A: Composition per liter: Agar ............................................................................................ 15.0g NaH2PO4·12H2O........................................................................... 9.0g KH2PO4......................................................................................... 1.5g

1916

Xanthomonas Agar

NH4·Cl .......................................................................................... 1.0g Sodium propionate or 3-hydroxybutyrate..................................... 1.0g MgSO4·7H2O ................................................................................ 0.2g Trace elements solution .............................................................1.0mL pH 7.0 ± 0.2 at 25°C

Trace Elements Solution: Composition per 2.0mL: H3BO4 .....................................................................................560.0μg ZnSO4·7H2O ...........................................................................350.0μg NiCl2·H2O ...............................................................................160.0μg Na2MoO4·2H2O ......................................................................100.0μg CuSO4·5H2O .............................................................................16.0μg MnCl2·4H2O..............................................................................16.0μg

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 2.0mL. Mix thoroughly.

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Solution B: Composition per 100.0mL: Ferric ammonium citrate.......................................................... 50.0mg CaCl2·2H2O............................................................................ 100.0mg

Preparation of Solution B: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Preparation of Medium: Aseptically combine 1.0L of sterile solution A with 100.0mL of sterile solution B. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation of Xanthobacter agilis.

Xanthomonas Agar Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of gelatin ......................................................... 10.0g Sucrose........................................................................................ 10.0g Beef extract ................................................................................... 6.0g pH 6.8 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Alcaligenes latus, Erwinia tracheiphila, Pseudomonas amygdali, Xanthomonas albilineans, Xanthomonas axonopodis, Xanthomonas campestris, Xanthomonas fragariae, Xanthomonas maltophilia, Xanthomonas oryzae, and Xylophilus ampelinus.

Xanthomonas albilineans Agar Composition per liter: Sucrose........................................................................................ 20.0g Agar ............................................................................................ 15.0g Peptone ....................................................................................... 10.0g Yeast extract.................................................................................. 5.0g pH 7.0 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Xanthomonas alblinieans.

Xanthomonas maltophilia Medium Composition per liter:

Trizma® (tris[hydroxymethyl] aminomethane) base .................. 6.04g Glucose ......................................................................................... 5.0g KCl................................................................................................ 1.0g NaCl.............................................................................................. 1.0g L-Phenylalanine ............................................................................ 0.9g MgSO4 .......................................................................................... 0.2g L-Arginine..................................................................................... 0.1g L-Methionine................................................................................. 0.1g (NH4)2SO4 .................................................................................... 0.1g NH4Cl ........................................................................................... 0.1g Glycerol ...................................................................................... 0.68g L-Serine....................................................................................... 0.22g L-Alanine .................................................................................... 0.18g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Filter sterilize. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Stenotrophomonas maltophilia.

Use: For the cultivation and maintenance of Xanthomonas species.

Xanthomonas Agar Composition per liter: CaCO3 ......................................................................................... 30.0g Agar ............................................................................................ 15.0g Glucose ....................................................................................... 10.0g Yeast extract.................................................................................. 5.0g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Cool rapidly. © 2010 by Taylor and Francis Group, LLC

Xanthomonas Medium Composition per liter: Pancreatic digest of gelatin......................................................... 10.0g Sucrose........................................................................................ 10.0g Beef extract................................................................................... 6.0g pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat with mixing. Distribute into screw-capped test tubes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Xanthomonas species.

Xanthomonas TYG Agar (Xanthomonas Tryptone Yeast Extract Glucose Agar) Composition per liter: Agar ............................................................................................ 20.0g Pancreatic digest of casein............................................................ 5.0g

XED-AGAR

Glucose ......................................................................................... 5.0g Yeast extract.................................................................................. 3.0g K2HPO4 ......................................................................................... 0.7g MgSO4·7H2O .............................................................................. 0.25g

1917

p-Aminobenzoic acid............................................................... 80.0mg D(+)-Biotin............................................................................... 20.0mg

Preparation of Solution C: Add components to distilled/deionized

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Sparge with 100% N2. Mix thoroughly. Filter sterilize.

Solution D: Composition per liter:

Use: For the cultivation and maintenance of Xanthomonas species.

XB45/XB90/PB90-2 Medium (DSMZ Medium 862) Composition per 1069.2mL: Solution A ..............................................................................940.0mL Solution E ..............................................................................100.0mL Solution D ................................................................................10.0mL Solution G ................................................................................10.0mL Solution F...................................................................................7.2mL Solution B ..................................................................................1.0mL Solution C ..................................................................................1.0mL pH 7.2 ± 0.2 at 25°C

Solution A: Composition per 940.0mL: NaCl .............................................................................................. 1.0g KCl................................................................................................ 0.5g MgCl2·6H2O.................................................................................. 0.4g KH2PO4 ......................................................................................... 0.2g NH4Cl ......................................................................................... 0.25g CaCl2·2H2O................................................................................. 0.15g Resazurin ................................................................................... 0.5mg

Preparation of Solution A: Prepare under 80% N2 + 20% CO2 gas atmosphere. Add components to distilled/deionized water and bring volume to 940.0mL. Mix thoroughly. Adjust pH to 7.2. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Solution B: Composition per liter: FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O........................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution) ..................................................................10.0mL

Preparation of Solution B: Add FeCl2·4H2O to 10.0mL of HCl so-

lution. Mix thoroughly. Add distilled/deionized water and bring volume to 1.0L. Add remaining components. Mix thoroughly. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Solution C: Composition per liter: Pyridoxine hydrochloride ...................................................... 300.0mg Thiamine-HCl·2H2O .............................................................. 200.0mg Nicotinic acid ......................................................................... 200.0mg Vitamin B12 ............................................................................ 100.0mg Calcium pantothenate ............................................................ 100.0mg © 2010 by Taylor and Francis Group, LLC

Preparation of Solution D: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Solution E: Composition per 100.0mL: NaHCO3 ........................................................................................ 5.0g

Preparation of Solution E: Add NaHCO3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Solution F: Composition per 10.0mL: Glucose ......................................................................................... 1.0g

Preparation of Solution F: Add glucose to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Solution G: Composition per 10.0mL: Na2S·9H2O................................................................................ 0.125g

Preparation of Solution G: Add Na2S·9H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to 25°C. Preparation of Medium: Prepare and dispense medium under 80% N2 + 20% CO2 gas atmosphere. Sequentially add 1.0mL solution B, 1.0mL solution C, 10.0mL solution D, 100.0mL solution E, 7.2mL solution F, and 10.0mL solution G to 940.0mL solution A. Distribute anaerobically under 80% N2 + 20% CO2 into appropriate vessels. The pH should be 7.2.

Use: For the cultivation of unclassified bacteria DSM 12558, DSM 12559, and DSM 12595.

XED-AGAR (DSMZ Medium 1026) Composition per liter: Agar ........................................................................................... 18.0g Xylan ............................................................................................ 7.0g Yeast extract ................................................................................. 3.0g pH 7.0 ± 0.2 at 25°C

1918

Xenorhabdus Agar

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Microbacterium ulmi.

980.0mL. Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks. Autoclave for 10 min at 14 psi pressure–118°C. Cool to 55°C. Aseptically add 20.0 mL of the sterile thiosulfate-citrate solution. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation, cultivation, and differentiation of enteric patho-

Xenorhabdus Agar Composition per liter: Agar ............................................................................................ 15.0g Peptone........................................................................................ 10.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.2. Distribute into tubes or flasks. Autoclave for 20 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Bacteroides galacturonicus and Xenorhabdus nematophilus.

Xenorhabdus Broth Composition per liter: Peptone........................................................................................ 10.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 5.0g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Distribute into tubes or flasks. Autoclave for 20 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Bacteroides galacturonicus and Xenorhabdus nematophilus.

XL Agar Base (Xylose Lysine Agar Base) Composition per liter: Agar ............................................................................................ 13.5g Lactose .......................................................................................... 7.5g Sucrose.......................................................................................... 7.5g L-Lysine......................................................................................... 5.0g NaCl .............................................................................................. 5.0g Xylose ........................................................................................... 3.5g Yeast extract.................................................................................. 3.0g Phenol Red .................................................................................. 0.08g Thiosulfate-citrate solution ......................................................20.0mL pH 7.5 ± 0.2 at 25°C

gens. Nonfermenting xylose/lactose/sucrose bacteria appear as red colonies. Xylose-fermenting, lysine-decarboxylating bacteria appear as red colonies. Xylose-fermenting, lysine-nondecarboxylating bacteria appear as opaque yellow colonies. Lactose- or sucrose-fermenting bacteria appear as yellow colonies.

XL Agar Base Composition per liter: Agar .............................................................................................. 15 g Lactose.......................................................................................... 7.5g Sucrose.......................................................................................... 7.5g L-Lysine......................................................................................... 5.0g NaCl.............................................................................................. 5.0g Xylose ......................................................................................... 3.75g Yeast extract.................................................................................. 3.0g Phenol Red.................................................................................. 0.08g Thiosulfate-citrate solution......................................................20.0mL pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems. Thiosulfate-Citrate Solution: Composition per 100.0mL: Na2S2O3 ...................................................................................... 34.0g Ferric ammonium citrate............................................................... 4.0g

Preparation of Thiosulfate-Citrate Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Preparation of Medium: Add components, except thiosulfate-citrate solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks. Autoclave for 10 min at 14 psi pressure–118°C. Cool to 55°C. Aseptically add 20.0 mL of the sterile thiosulfate-citrate solution. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation, cultivation, and differentiation of enteric pathogens. Nonfermenting xylose/lactose/sucrose bacteria appear as red colonies. Xylose-fermenting, lysine-decarboxylating bacteria appear as red colonies. Xylose-fermenting, lysine-nondecarboxylating bacteria appear as opaque yellow colonies. Lactose- or sucrose-fermenting bacteria appear as yellow colonies.

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Thiosulfate-Citrate Solution: Composition per 100.0mL: Na2S2O3 ...................................................................................... 34.0g Ferric ammonium citrate............................................................... 4.0g

XLD Agar (Xylose Lysine Deoxycholate Agar) Composition per liter: Agar ............................................................................................ 13.5g Lactose.......................................................................................... 7.5g Sucrose.......................................................................................... 7.5g Na2S2O3 ........................................................................................ 6.8g L-Lysine......................................................................................... 5.0g NaCl.............................................................................................. 5.0g Xylose ........................................................................................... 3.5g

XLT4 HiVeg Agar Base

Yeast extract.................................................................................. 3.0g Sodium desoxycholate .................................................................. 2.5g Ferric ammonium citrate............................................................... 0.8g Phenol Red .................................................................................. 0.08g pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems and Oxoid Unipath.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Do not overheat. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Plates should be poured as soon as possible to avoid precipitation.

Use: For the isolation and differentiation of enteric pathogens, especially Shigella and Providencia species. Nonfermenting xylose/lactose/sucrose bacteria appear as red colonies. Xylose-fermenting, lysinedecarboxylating bacteria appear as red colonies. Xylose-fermenting, lysine-nondecarboxylating bacteria appear as opaque yellow colonies. Lactose- or sucrose-fermenting bacteria appear as yellow colonies.

XLD Agar (Xylose Lysine Deoxycholate Agar) (BAM M179) Composition per liter: Agar ............................................................................................ 15.0g Lactose .......................................................................................... 7.5g Sucrose.......................................................................................... 7.5g Na2S2O3 ........................................................................................ 6.8g L-Lysine......................................................................................... 5.0g NaCl .............................................................................................. 5.0g Xylose ......................................................................................... 3.75g Yeast extract.................................................................................. 3.0g Sodium deoxycholate.................................................................... 2.5g Ferric ammonium citrate............................................................... 0.8g Phenol Red .................................................................................. 0.08g pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems and Oxoid. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Do not overheat. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Plates should be poured as soon as possible to avoid precipitation.

Use: For the isolation and differentiation of enteric pathogens, especially Shigella and Providencia species. Nonfermenting xylose/lactose/sucrose bacteria appear as red colonies. Xylose-fermenting, lysine-decarboxylating bacteria appear as red colonies. Xylose-fermenting, lysine-nondecarboxylating bacteria appear as opaque yellow colonies. Lactose- or sucrose-fermenting bacteria appear as yellow colonies.

XLD Agar, HiVeg (Xylose Lysine Deoxycholate HiVeg Agar) Composition per liter: Agar ............................................................................................ 15.0g Lactose .......................................................................................... 7.5g Sucrose.......................................................................................... 7.5g © 2010 by Taylor and Francis Group, LLC

1919

Na2S2O3 ........................................................................................ 6.8g L-Lysine ........................................................................................ 5.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 4.0g Xylose ........................................................................................... 3.5g Synthetic detergent No. III ........................................................... 1.5g Ferric ammonium citrate............................................................... 0.8g Phenol Red.................................................................................. 0.08g Selective supplement solution ...................................................4.6mL pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Selective Supplement Solution: Composition per 100.0mL: Tergitol™ 4........................................................................ Proprietary

Preparation of Selective Supplement Solution: Available as premixed solution.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Do not overheat. Distribute into tubes or flasks. Do not autoclave. Pour into sterile Petri dishes or leave in tubes. Plates should be poured as soon as possible to avoid precipitation.

Use: For the isolation and differentiation of enteric pathogens, especially Shigella and Providencia species. Nonfermenting xylose/lactose/sucrose bacteria appear as red colonies. Xylose-fermenting, lysine-decarboxylating bacteria appear as red colonies. Xylose-fermenting, lysine-nondecarboxylating bacteria appear as opaque yellow colonies. Lactose- or sucrose-fermenting bacteria appear as yellow colonies.

XLT4 HiVeg Agar Base Composition per liter: Agar ............................................................................................ 18.0g Lactose.......................................................................................... 7.5g Saccharose .................................................................................... 7.5g Na2S2O3 ........................................................................................ 6.8g L-Lysine ........................................................................................ 5.0g NaCl.............................................................................................. 5.0g Xylose ......................................................................................... 3.75g Yeast extract.................................................................................. 3.0g Plant peptone No. 3....................................................................... 1.6g Ferric ammonium citrate............................................................... 0.8g Phenol Red.................................................................................. 0.08g Selective supplement solution ...................................................4.6mL pH 7.4 ± 0.2 at 25°C

Source: This medium, without selective supplement solution, is available as a premixed powder from HiMedia.

Selective Supplement Solution: Composition per 100.0mL: Tergitol™ 4........................................................................ Proprietary

Preparation of Selective Supplement Solution: Available as premixed solution.

1920

XPS Agar

Use: For the isolation and differentiation of enteric pathogens, especially Shigella and Providencia species.

Potatoes, Infusion From: Composition per 500.0mL: Potatoes......................................................................................... 4.0g

XPS Agar Composition per liter: Solution A ..............................................................................500.0mL Solution B ..............................................................................500.0mL pH 5.1 ± 0.2 at 25°C

Solution A: Composition per 500.0mL: Potatoes, infusion from ............................................................... 40.0g Sucrose........................................................................................ 15.0g Peptone.......................................................................................... 5.0g Glucose ......................................................................................... 4.0g Casamino acids ............................................................................. 1.0g Na2HPO4 ..................................................................................... 0.79g Ca(NO3)2·4H2O solution..........................................................10.0mL

Potatoes, Infusion From: Composition per 500.0mL: Potatoes ......................................................................................... 4.0g

Preparation of Potatoes, Infusion From: Peel and dice potatoes. Add 400.0mL of distilled/deionized water. Gently heat and bring to boiling. Continue boiling for 30 min. Filter through cheesecloth.

Ca(NO3)2·4H2O Solution: Composition per 10.0mL: Ca(NO3)2·4H2O............................................................................. 0.5g

Preparation of Ca(NO3)2·4H2O Solution: Add Ca(NO3)2·4H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Solution A: Add components, except Ca(NO3)2·4H2O solution, to distilled/deionized water and bring volume to 490.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0mL of sterile Ca(NO3)2·4H2O solution. Mix thoroughly. Cool to 50°–55°C. Solution B: Composition per 500.0mL: Agar ............................................................................................ 20.0g

Preparation of Solution A: Add agar to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Preparation of Medium: Aseptically combine 500.0mL of solution A with 500.0mL of solution B. Mix thoroughly. Aseptically pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Xanthomonas campestris.

XPS Broth Composition per liter: Potatoes, infusion from ............................................................... 40.0g Sucrose........................................................................................ 15.0g Peptone.......................................................................................... 5.0g Glucose ......................................................................................... 4.0g Casamino acids ............................................................................. 1.0g Na2HPO4 ..................................................................................... 0.79g Ca(NO3)2·4H2O solution..........................................................10.0mL pH 5.1 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

Preparation of Potatoes, Infusion From: Peel and dice potatoes. Add 500.0mL of distilled/deionized water. Gently heat and bring to boiling. Continue boiling for 30 min. Filter through cheesecloth.

Ca(NO3)2·4H2O Solution: Composition per 10.0mL: Ca(NO3)2·4H2O ............................................................................ 0.5g

Preparation of Ca(NO3)2·4H2O Solution: Add Ca(NO3)2·4H2O

to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except Ca(NO3)2·4H2O solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0mL of sterile Ca(NO3)2·4H2O solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Xanthomonas campestris.

XPS Broth with Thymidine (Thymidine Auxotroph XPS Medium) Composition per liter: Potatoes, infusion from............................................................... 40.0g Sucrose........................................................................................ 15.0g Peptone ......................................................................................... 5.0g Glucose ......................................................................................... 4.0g Casamino acids ............................................................................. 1.0g Na2HPO4 ..................................................................................... 0.79g Thymidine................................................................................ 10.0mg Ca(NO3)2·4H2O solution .........................................................10.0mL pH 5.1 ± 0.2 at 25°C

Potatoes, Infusion From: Composition per 500.0mL: Potatoes......................................................................................... 4.0g

Preparation of Potatoes, Infusion From: Peel and dice potatoes. Add 500.0mL of distilled/deionized water. Gently heat and bring to boiling. Continue boiling for 30 min. Filter through cheesecloth.

Ca(NO3)2·4H2O Solution: Composition per 10.0mL: Ca(NO3)2·4H2O ............................................................................ 0.5g

Preparation of Ca(NO3)2·4H2O Solution: Add Ca(NO3)2·4H2O

to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Use: For the cultivation of Xanthomonas oryzae.

XSM Agar Composition per liter: Agar ............................................................................................ 15.0g Glucose ......................................................................................... 5.0g Sucrose.......................................................................................... 2.0g Malt extract................................................................................... 1.0g

Xylella Agar

Yeast extract.................................................................................. 1.0g Liver extract concentrate .............................................................. 1.0g Corn steep liquor........................................................................... 1.0g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to tap water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into screw-capped test tubes. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Streptomyces cinereus and Streptomyces flaveus.

Xylan Medium Composition per liter: Xylan........................................................................................... 30.0g Agar ............................................................................................ 12.0g Peptone.......................................................................................... 2.0g Yeast extract.................................................................................. 0.5g L-Cysteine·HCl·H2O ................................................................... 0.25g Na2S·9H2O .................................................................................. 0.25g Rumen fluid ...........................................................................400.0mL NaHCO3 solution .....................................................................40.0mL Mineral solution I.....................................................................25.0mL Mineral solution II ...................................................................25.0mL Wolfe’s vitamin solution ..........................................................10.0mL VFA solution............................................................................10.0mL Hemin solution.........................................................................10.0mL Trace elements solution SL-6 ....................................................1.0mL pH 7.0 ± 0.2 at 25°C

NaHCO3 Solution: Composition per 100.0mL: NaHCO3 ...................................................................................... 3.96g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/de-

ionized water and bring volume to 100.0mL. Mix thoroughly. Gas with 100% CO2.

Mineral Solution I: Composition per liter: K2HPO4 ......................................................................................... 3.0g

Preparation of Mineral Solution I: Add K2HPO4 to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Mineral Solution II: Composition per liter: Sodium citrate ............................................................................. 20.0g NaCl ............................................................................................ 12.0g KH2PO4 ......................................................................................... 6.0g MgCl2·6H2O.................................................................................. 2.0g CaCl2 ............................................................................................. 1.2g

Preparation of Mineral Solution II: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Wolfe’s Vitamin Solution: Composition per liter: Pyridoxine·HCl ........................................................................ 10.0mg Thiamine·HCl ............................................................................ 5.0mg Riboflavin .................................................................................. 5.0mg Nicotinic acid ............................................................................. 5.0mg Calcium pantothenate ................................................................ 5.0mg p-Aminobenzoic acid................................................................. 5.0mg Thioctic acid .............................................................................. 5.0mg © 2010 by Taylor and Francis Group, LLC

1921

Biotin ......................................................................................... 2.0mg Folic acid ................................................................................... 2.0mg Cyanocobalamin ..................................................................... 100.0μg

Preparation of Wolfe’s Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. VFA Solution: Composition per liter: Acetic acid .............................................................................178.3mL Propionic acid ..........................................................................59.6mL n-Butyric acid ..........................................................................38.4mL Isobutyric acid ...........................................................................9.5mL n-Valeric acid .............................................................................9.4mL Isovaleric acid............................................................................9.3mL DL-α-Methylbutyric acid ...........................................................4.4mL

Preparation of VFA Solution: Add components to distilled/deionized water and bring volume to approximately 500.0mL. Adjust pH to 7.5 with NaOH. Mix thoroughly. Bring volume to 1.0L with distilled/ deionized water. Hemin Solution: Composition per 100.0mL: Hemin ......................................................................................... 0.01g

Preparation of Hemin Solution: Add hemin to 100.0mL of 0.01N NaOH. Mix thoroughly.

Trace Elements Solution SL-6: Composition per liter: H3BO3 ........................................................................................... 0.3g CoCl2·6H2O .................................................................................. 0.2g ZnSO4·7H2O ................................................................................. 0.1g MnCl2·4H2O ............................................................................... 0.03g Na2MoO4·H2O ............................................................................ 0.03g NiCl2·6H2O................................................................................. 0.02g CuCl2·2H2O................................................................................. 0.01g

Preparation of Trace Elements Solution SL-6: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3.4.

Preparation of Medium: Add components, except Na2S·9H2O,

NaCHO3,and L-cysteine·HCl·H2O solutions, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Cool under 80% N2 + 20% CO2. Add L-cysteine·HCl·H2O and Na2S·9H2O. Add sufficient NaCHO3 solution to bring pH to 7.2 under 80% N2 + 20% CO2. Anaerobically distribute into tubes under 80% N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Clostridium xylanolyticum and other microorganisms that can utilize xylan as a carbon source.

Xylella Agar (LMG Medium 115) Composition per liter: Agar ............................................................................................ 17.0g Yeast extract................................................................................ 10.0g ACES .......................................................................................... 10.0g Activated charcoal ........................................................................ 2.0g α-ketoglutarate.............................................................................. 1.0g KOH, 1N .....................................................................................40mL L-Cysteine-iron solution ..........................................................20.0mL pH 6.9 ± 0.2 at 25°C

1922

Xylella fastidiosa Medium

L-Cysteine-Iron

Solution: Composition per 20.0mL:

L-Cysteine·HCl.............................................................................. 0.4g

Fe4(P2O7)3 ................................................................................... 0.25g

Preparation of L-Cysteine-Iron Solution: Add components to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add ACES to 500.0mL of distilled water at 50°C. Combine with a solution containing 40.0mL of 1N KOH in 440.0mL of distilled water. Add the other components except cysteine iron solution. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 20.0mL sterile cysteine iron solution. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation of Xylella spp.

Xylella fastidiosa Medium (LMG 115) Composition per liter: Agar ............................................................................................ 17.0g Yeast extract................................................................................ 10.0g ACES buffer................................................................................ 10.0g Activated charcoal ........................................................................ 2.0g L-Cysteine-iron solution...........................................................20.0mL pH 6.9 ± 0.2 at 25°C

Preparation of Medium: Add components, except ferric ammonium citrate solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add 10.0mL of sterile ferric ammonium citrate solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Xylophilus ampelina.

Xylose Lactose Tergitol™ 4 (XLT-4) Composition per 1004.6mL: Agar ............................................................................................ 18.0g Lactose.......................................................................................... 7.5g Sucrose.......................................................................................... 7.5g Na2S2O3 ........................................................................................ 6.8g Lysine............................................................................................ 5.0g NaCl.............................................................................................. 5.0g Xylose ......................................................................................... 3.75g Yeast extract.................................................................................. 3.0g Proteose peptone........................................................................... 1.6g Ferric ammonium citrate............................................................... 0.8g Phenol Red.................................................................................. 0.08g Selective supplement solution ...................................................4.6mL pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid Unipath.

L-Cysteine-Iron

Selective Supplement Solution: Composition per 100.0mL:

L-Cysteine·HCl.............................................................................. 0.4g Fe4(P2O7)3 ................................................................................... 0.25g

Tergitol™ 4........................................................................ Proprietary

Preparation of L-Cysteine-Iron Solution: Add components to

premixed solution.

distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except selective supplement solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Add 4.6mL of selective supplement solution. Mix thoroughly. Gently heat while stirring and bring to boiling. Do not autoclave. Cool to 50°C. Mix thoroughly. Pour into sterile Petri dishes.

Solution: Composition per 20.0mL:

Preparation of Medium: Add ACES to 500.0mL of distilled/deionized water at 50°C. Add a solution containing 40.0mL of 1N KOH in 440.0mL of distilled water. Mix thoroughly. Add the remaining components, except L-cysteine-iron solution. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 6.9 with KOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 20.0mL of sterile L-cysteine-iron solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation of Xylella fastidiosa.

Xylophilus Medium Composition per liter: CaCO3 ......................................................................................... 20.0g Agar ............................................................................................ 15.0g D-Galactose ................................................................................. 10.0g Yeast extract................................................................................ 10.0g Ferric ammonium citrate solution............................................10.0mL

Ferric Ammonium Citrate Solution: Composition per 10.0mL:

Preparation of Selective Supplement Solution: Available as a

Use: For the isolation and identification of salmonellae from clinical, environmental, and food samples. The presence of the selective agent, Tergitol™ 4, in this medium inhibits many organisms that can be problematic on other plating media. In addition, biochemical and pH changes within the medium allow Salmonella spp. (black colonies) to be differentiated from organisms such as E. coli (yellow colonies) and Shigella spp. (red colonies). The enhanced selectivity of XLT-4 Agar reduces the need for further identification procedures, saving time and money, and results in fewer false presumptive positive colonies when compared to other Salmonella plating media. Xylose Lysine Agar Base See: XL Agar Base Xylose Lysine Desoxycholate Agar See: XLD Agar

Xylose Sodium Deoxycholate Citrate Agar

Ferric ammonium citrate............................................................. 0.25g

Composition per liter:

Preparation of Ferric Ammonium Citrate Solution: Add fer-

Agar ............................................................................................ 12.0g Xylose ......................................................................................... 10.0g Sodium citrate............................................................................... 5.0g Na2S2O3·5H2O .............................................................................. 5.0g

Y 1 Adrenal Cell Growth Medium

Beef extract ................................................................................... 5.0g Peptone.......................................................................................... 5.0g NaCl .............................................................................................. 2.5g Sodium deoxycholate.................................................................... 2.5g Ferric ammonium citrate............................................................... 1.0g Neutral Red (1% solution) .........................................................2.5mL pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling for 20 sec. Do not autoclave. Cool to 45°–50°C. Pour into sterile Petri dishes.

Use: For the cultivation of Salmonella species and some Shigella species.

Xylose YP Agar (Xylose Yeast Extract Peptone Agar) Composition per liter: CaCO3 ......................................................................................... 20.0g Agar ............................................................................................ 15.0g Xylose ......................................................................................... 10.0g Yeast extract................................................................................ 10.0g Peptone........................................................................................ 10.0g MgSO4·7H2O ................................................................................ 0.2g MnSO4·4H2O .............................................................................. 0.01g FeSO4·7H2O................................................................................ 0.01g NaCl ............................................................................................ 0.01g pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into screw-capped test tubes. Autoclave for 15 min at 15 psi pressure–121°C. Adjust pH to 6.8. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Lactobacillus vaccinostercus and other microorganisms that utilize xylose as a carbon source.

Xylose YP Broth (Xylose Yeast Extract Peptone Broth) Composition per liter: Xylose ......................................................................................... 10.0g Yeast extract................................................................................ 10.0g Peptone........................................................................................ 10.0g MgSO4·7H2O ................................................................................ 0.2g MnSO4·4H2O .............................................................................. 0.01g FeSO4·7H2O................................................................................ 0.01g NaCl ............................................................................................ 0.01g pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into screwcapped test tubes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Lactobacillus vaccinostercus and other microorganisms that utilize xylose as a carbon source.

Y 1 Adrenal Cell Growth Medium

1923

Ham’s F-10 Medium: Composition per liter: NaCl.............................................................................................. 7.4g NaHCO3 ........................................................................................ 1.2g Glucose ......................................................................................... 1.1g NaH2PO4·H2O............................................................................. 0.29g KCl.............................................................................................. 0.28g L-Arginine·HCl............................................................................ 0.21g L-Glutamine ................................................................................ 0.15g MgSO4·7H2O .............................................................................. 0.15g Sodium pyruvate......................................................................... 0.11g KH2PO4....................................................................................... 0.08g CaCl2·2H2O ................................................................................ 0.04g L-Cystine·2HCl ........................................................................... 0.04g L-Histidine·HCl·H2O................................................................... 0.02g L-Lysine·HCl ............................................................................... 0.02g L-Asparagine-H2O....................................................................... 0.01g L-Aspartic Acid ........................................................................... 0.01g L-Glutamic acid........................................................................... 0.01g L-Leucine .................................................................................... 0.01g L-Proline...................................................................................... 0.01g L-Serine ....................................................................................... 0.01g L-Alanine.................................................................................... 8.9mg Glycine....................................................................................... 7.5mg D-Phenylalanine ......................................................................... 5.0mg L-Methionine .............................................................................. 4.5mg Hypoxanthine............................................................................. 4.1mg L-Threonine ................................................................................ 3.6mg L-Valine ..................................................................................... 3.5mg L-Isoleucine ................................................................................ 2.6mg L-Tyrosine .................................................................................. 1.8mg Vitamin B12 ................................................................................ 1.4mg Folic acid ................................................................................... 1.3mg Phenol Red................................................................................. 1.2mg Thiamine·HCl ............................................................................ 1.0mg FeSO4·7H2O............................................................................... 0.8mg Choline chloride......................................................................... 0.7mg D-Calcium pantothenate ............................................................. 0.7mg Thymidine.................................................................................. 0.7mg Niacinamide............................................................................... 0.6mg L-Tryptophan.............................................................................. 0.6mg Isoinositol .................................................................................. 0.5mg Riboflavin .................................................................................. 0.4mg Lipoic acid ................................................................................. 0.2mg Pyridoxine·HCl .......................................................................... 0.2mg ZnSO4·7H2O ............................................................................ 0.03mg Biotin ....................................................................................... 0.02mg CuSO4·5H2O............................................................................... 3.0μg

Preparation of Ham’s F-10 Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Penicillin-Streptomycin Solution: Composition per 100.0mL: Streptomycin................................................................................. 0.5g Penicillin G .......................................................................... 500,000U

Composition per 101.0mL:

Preparation of Penicillin-Streptomycin Solution: Add compo-

Ham’s F-10 medium ................................................................90.0mL Fetal bovine serum...................................................................10.0mL Penicillin-streptomycin solution ................................................1.0mL pH 7.0 ± 0.2 at 25°C

sterilize. Store at 4–5°C.

nents to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Aseptically combine components. Filter

1924

Y 1 Adrenal Cell Growth Medium

Use: For the cultivation of Y-1 mouse adrenal tissue culture cells used for the detection of heat-labile toxin (LT) produced by enterotoxigenic strains of Escherichia coli. LT causes the conversion of elongated fibroblast-like cells into round, refractile cells.

Y 1 Adrenal Cell Growth Medium Composition per 580.0mL:

Penicillin-Streptomycin Solution: Composition per 100.0mL: Streptomycin................................................................................. 0.5g Penicillin G .......................................................................... 500,000U

Preparation of Penicillin-Streptomycin Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Ham’s F-10 medium ..............................................................500.0mL Fetal bovine serum...................................................................75.0mL Penicillin-streptomycin solution ................................................5.0mL pH 7.0 ± 0.2 at 25°C

sterilize. Store at 4°–5°C.

Ham’s F-10 Medium: Composition per liter:

the detection of cholera enterotoxin (CT) produced by enterotoxigenic strains of Vibrio cholerae or Vibrio mimicus. CT causes the conversion of elongated fibroblast-like cells into round, refractile cells.

NaCl .............................................................................................. 7.4g NaHCO3 ........................................................................................ 1.2g Glucose ......................................................................................... 1.1g NaH2PO4·H2O............................................................................. 0.29g KCl.............................................................................................. 0.28g L-Arginine·HCl............................................................................ 0.21g L-Glutamine................................................................................. 0.15g MgSO4·7H2O .............................................................................. 0.15g Sodium pyruvate ......................................................................... 0.11g KH2PO4 ....................................................................................... 0.08g CaCl2·2H2O................................................................................. 0.04g L-Cystine·2HCl............................................................................ 0.04g L-Histidine·HCl·H2O ................................................................... 0.02g L-Lysine·HCl ............................................................................... 0.02g L-Asparagine-H2O....................................................................... 0.01g L-Aspartic Acid ........................................................................... 0.01g L-Glutamic acid ........................................................................... 0.01g L-Leucine..................................................................................... 0.01g L-Proline ...................................................................................... 0.01g L-Serine ....................................................................................... 0.01g L-Alanine.................................................................................... 8.9mg Glycine....................................................................................... 7.5mg D-Phenylalanine.......................................................................... 5.0mg L-Methionine .............................................................................. 4.5mg Hypoxanthine............................................................................. 4.1mg L-Threonine ................................................................................ 3.6mg L-Valine ..................................................................................... 3.5mg L-Isoleucine ................................................................................ 2.6mg L-Tyrosine................................................................................... 1.8mg Vitamin B12 ................................................................................ 1.4mg Folic acid.................................................................................... 1.3mg Phenol Red ................................................................................. 1.2mg Thiamine·HCl ............................................................................ 1.0mg FeSO4·7H2O............................................................................... 0.8mg Choline chloride......................................................................... 0.7mg D-Calcium pantothenate ............................................................. 0.7mg Thymidine .................................................................................. 0.7mg Niacinamide ............................................................................... 0.6mg L-Tryptophan .............................................................................. 0.6mg Isoinositol................................................................................... 0.5mg Riboflavin .................................................................................. 0.4mg Lipoic acid ................................................................................. 0.2mg Pyridoxine·HCl .......................................................................... 0.2mg ZnSO4·7H2O ............................................................................ 0.03mg Biotin ....................................................................................... 0.02mg CuSO4·5H2O ...............................................................................3.0μg

Preparation of Medium: Aseptically combine components. Filter Use: For the cultivation of Y-1 mouse adrenal tissue culture cells used for

YA12 Composition per liter: Agar ............................................................................................ 10.0g Glucose ......................................................................................... 0.6g NaCl.............................................................................................. 0.5g Beef extract................................................................................... 0.2g Yeast extract................................................................................ 0.02g pH 6.5–6.7 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.5–6.7. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Adelphamoeba galeacystis.

YA Halophile Medium Composition per liter: NaCl.......................................................................................... 100.0g Agar ............................................................................................ 15.0g Sodium acetate·3H2O.................................................................. 10.0g Na2HPO4 ....................................................................................... 3.8g KH2PO4......................................................................................... 1.3g Mg(NO3)2·6H2O ........................................................................... 1.0g (NH4)2SO4 .................................................................................... 1.0g Yeast extract.................................................................................. 1.0g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except magnesium nitrate, to tap water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add magnesium nitrate. Adjust pH 7.2 with sterile KOH. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of halophilic microorganisms, including Bacillus halodenitrificans.

YB Medium (Yeast Extract Beef Extract Medium) Composition per liter: Agar ............................................................................................ 20.0g Peptone ....................................................................................... 10.0g Beef extract................................................................................... 7.0g Yeast extract.................................................................................. 5.0g NaCl.............................................................................................. 3.0g Thiourea........................................................................................ 0.1g Methanol ..................................................................................20.0mL pH 7.2 ± 0.2 at 25°C

YC Medium without Tryptophan

1925

Preparation of Medium: Add components, except methanol, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Aseptically add filter-sterilized methanol. Pour into sterile Petri dishes or leave in tubes.

Preparation of Solution A: Add components to distilled/deionized

Use: For the cultivation and maintenance of bacteria that can utilize methanol as a carbon source, including Achromobacter methanolophila, Methanomonas methylovora, Methylobacterium species, and Pseudomonas methanolica.

Agar, noble.................................................................................. 20.0g

YC Agar without Tryptophan Composition per liter:

water and bring volume to 500.0mL. Mix thoroughly. Adjust pH to 5.6–5.7. Filter sterilize. Warm to 50°–55°C.

Solution B: Composition per 500.0mL: Preparation of Solution B: Add agar to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.

Preparation of Medium: Aseptically mix 500.0mL of solution A

Solution A ..............................................................................500.0mL Solution B ..............................................................................500.0mL

and 500.0mL of solution B. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Solution A: Composition per 500.0mL:

siae.

Glucose ....................................................................................... 20.0g Succinic acid ............................................................................... 10.0g NaOH ............................................................................................ 6.0g (NH4)2SO4 .................................................................................... 5.0g KH2PO4 ......................................................................................... 0.6g MgSO4·7H2O ................................................................................ 0.3g Adenine ......................................................................................... 0.1g L-Arginine ..................................................................................... 0.1g L-Cysteine ..................................................................................... 0.1g L-Leucine ...................................................................................... 0.1g L-Lysine......................................................................................... 0.1g L-Threonine................................................................................... 0.1g Uracil ............................................................................................ 0.1g L-Aspartic acid ............................................................................ 0.05g L-Histidine................................................................................... 0.05g L-Isoleucine................................................................................. 0.05g L-Methionine............................................................................... 0.05g L-Phenylalanine........................................................................... 0.05g L-Proline...................................................................................... 0.05g L-Serine ....................................................................................... 0.05g L-Tyrosine ................................................................................... 0.05g L-Valine ....................................................................................... 0.05g NaCl ............................................................................................ 0.05g CaCl2·2H2O................................................................................. 0.05g DL-Methionine............................................................................. 0.01g L-Histidine·HCl ......................................................................... 0.005g Inositol ....................................................................................... 1.0mg KI ............................................................................................... 0.5mg H3BO3 ........................................................................................ 0.3mg ZnSO4·7H2O .............................................................................. 0.2mg MnSO4·4H2O ............................................................................. 0.2mg Thiamine·HCl ............................................................................ 0.2mg Pyroxidine·HCl .......................................................................... 0.2mg Niacin......................................................................................... 0.2mg Calcium pantothenate ................................................................ 0.2mg p-Aminobenzoic acid................................................................. 0.1mg Riboflavin .................................................................................. 0.1mg FeCl3 .......................................................................................... 0.1mg Na2MoO4·4H2O ......................................................................... 0.1mg CuSO4·5H2O ............................................................................ 0.02mg Folic acid.....................................................................................1.0μg Biotin ..........................................................................................1.0μg pH 5.7 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

Use: For the cultivation and maintenance of Saccharomyces cerevi-

YC Medium without Tryptophan Composition per liter: Solution A..............................................................................500.0mL Solution B ..............................................................................500.0mL

Solution A: Composition per 500.0mL: Glucose ....................................................................................... 20.0g Succinic acid............................................................................... 10.0g NaOH............................................................................................ 6.0g (NH4)2SO4 .................................................................................... 5.0g KH2PO4......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g NaCl.............................................................................................. 0.1g CaCl2·2H2O .................................................................................. 0.1g Adenine......................................................................................... 0.1g L-Arginine..................................................................................... 0.1g L-Cysteine ..................................................................................... 0.1g L-Leucine ...................................................................................... 0.1g L-Lysine ........................................................................................ 0.1g L-Threonine................................................................................... 0.1g Uracil ............................................................................................ 0.1g L-Aspartic acid............................................................................ 0.05g L-Histidine .................................................................................. 0.05g L-Isoleucine................................................................................. 0.05g L-Methionine............................................................................... 0.05g L-Phenylalanine .......................................................................... 0.05g L-Proline ..................................................................................... 0.05g L-Serine....................................................................................... 0.05g L-Tyrosine ................................................................................... 0.05g L-Valine....................................................................................... 0.05g Inositol ....................................................................................... 2.0mg KI ............................................................................................... 1.0mg H3BO3 ........................................................................................ 0.5mg ZnSO4·7H2O .............................................................................. 0.4mg MnSO4·4H2O ............................................................................. 0.4mg Thiamine·HCl ............................................................................ 0.4mg Pyroxidine·HCl .......................................................................... 0.4mg Niacin......................................................................................... 0.4mg Calcium pantothenate ................................................................ 0.4mg p-Aminobenzoic acid................................................................. 0.2mg Riboflavin .................................................................................. 0.2mg FeCl3 .......................................................................................... 0.2mg Na2MoO4·4H2O ......................................................................... 0.2mg

1926

YDC Agar

CuSO4·5H2O ............................................................................ 0.04mg Folic acid.....................................................................................2.0μg Biotin ..........................................................................................2.0μg

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 500.0mL. Adjust pH to 5.6–5.7. Filter sterilize. Warm to 50°–55°C. Solution B: Composition per 500.0mL: Agar, noble.................................................................................. 20.0g

Preparation of Solution B: Add agar to distilled/deionized water and bring volume to 500.0mL. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50–55°C.

Preparation of Medium: Aseptically combine 500.0mL of solution A and 500.0mL of solution B. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cultivation of Saccharomyces cerevisiae.

YDC Agar (Yeast Extract Dextrose Calcium Carbonate Agar) Composition per liter: CaCO3, finely divided................................................................. 20.0g Glucose ....................................................................................... 20.0g Agar ............................................................................................ 15.0g Yeast extract................................................................................ 10.0g pH 7. 0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to cool in a slanted position.

Use: For the cultivation and maintenance of Pseudomonas species on agar slants.

YDC Medium Composition per liter: CaCO3 ......................................................................................... 20.0g Glucose ....................................................................................... 20.0g Agar ............................................................................................ 15.0g Yeast extract................................................................................ 10.0g pH 7.2 ± 0.2 at 25°C

Use: For the cultivation of Bdellovibrio species.

Yeast Agar, Van Niel’s Composition per liter: Agar ............................................................................................ 20.0g Yeast extract................................................................................ 10.0g K2HPO4 ......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g pH 7.0–7.2 at 25°C

Use: For the cultivation and maintenance of a variety of microorganisms, including Cytophaga species, Heliobacterium chlorum, Rhodomicrobium vannielii, Lysobacter enzymogenes, Rhodobacter species, Rhodocyclus gelatinosus, Rhodopseudomonas palustris, and Rhodospirillum rubrum.

Yeast Agar, Van Niel’s with Glutamate Composition per liter: Agar ............................................................................................ 20.0g Yeast extract................................................................................ 10.0g K2HPO4......................................................................................... 1.0g MgSO4 .......................................................................................... 0.5g Monosodium glutamate .............................................................. 0.85g pH 7.0–7.2 at 25°C

Preparation of Medium: Add components to tap water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of a variety of bacteria, including Bacillus firmus, Cytophaga johnsonae, Heliobacterium chlorum, Lysobacter enzymogenes, Rhodobacter capsulatus, Rhodomicrobium vannielii, Rhodobacter sphaeroides, Rhodocyclus gelatinosus, Rhodocyclus gelatinosus, Rhodo-pseudomonas palustris, and Rhodospirillum rubrum.

Yeast Agar, Van Niel’s with 2.5% Sodium Chloride (ATCC Medium 1370) Composition per liter: NaCl............................................................................................ 25.0g Agar ............................................................................................ 20.0g Yeast extract................................................................................ 10.0g K2HPO4......................................................................................... 1.0g MgSO4 .......................................................................................... 0.5g pH 7.0–7.2 at 25°C

Yeast Agar, Van Niel’s with 25% Sodium Chloride Composition per liter: NaCl.......................................................................................... 250.0g Agar ............................................................................................ 20.0g Yeast extract................................................................................ 10.0g K2HPO4......................................................................................... 1.0g MgSO4 .......................................................................................... 0.5g pH 7.0–7.2 at 25°C

Yeast Extract Agar

Yeast Agar, Van Niel’s with Succinate Composition per liter: Agar ............................................................................................ 20.0g Yeast extract................................................................................ 10.0g Sodium succinate ........................................................................ 1.35g K2HPO4 ......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g pH 7.0–7.2 at 25°C

Yeast Ascospore Agar Composition per liter: Agar ............................................................................................ 30.0g Potassium acetate ........................................................................ 10.0g Yeast extract.................................................................................. 2.5g Glucose ......................................................................................... 1.0g

Use: For the cultivation and observation of ascospore formation of yeast.

Yeast Beef Agar See: Antibiotic Medium 4

Yeast Carbon Base, 10X (Wickerham Carbon Base Broth) Composition per liter: Glucose ....................................................................................... 10.0g KH2PO4 ......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g NaCl .............................................................................................. 0.1g CaCl2·2H2O................................................................................... 0.1g DL-Methionine............................................................................. 0.02g DL-Tryptophan............................................................................. 0.02g L-Histidine·HCl ........................................................................... 0.01g Inositol ....................................................................................... 2.0mg H3BO3 ........................................................................................ 0.5mg ZnSO4·7H2O .............................................................................. 0.4mg MnSO4·4H2O ............................................................................. 0.4mg Thiamine·HCl ............................................................................ 0.4mg Pyridoxine .................................................................................. 0.4mg Niacin......................................................................................... 0.4mg Calcium pantothenate ................................................................ 0.4mg p-Aminobenzoic acid................................................................. 0.2mg Riboflavin .................................................................................. 0.2mg FeCl3 .......................................................................................... 0.2mg Na2MoO4·4H2O ......................................................................... 0.2mg KI ............................................................................................... 0.1mg CuSO4·5H2O ............................................................................ 0.04mg Folic Acid ...................................................................................2.0μg Biotin ..........................................................................................2.0μg pH 5.5 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

1927

Use: Used as a base to which different nitrogen sources may be added. For the cultivation and differentiation of bacteria based on their ability to utilize diverse added nitrogen sources.

Yeast Dextrose Agar Composition per liter: Agar ............................................................................................ 15.0g Glucose ....................................................................................... 10.0g Yeast extract................................................................................ 10.0g pH 7.0 ± 0.2 at 25°C

Use: For the cultivation of a variety of heterotrophic microorganisms.

Yeast Extract Agar Composition per liter: Agar ............................................................................................ 15.0g Malt extract................................................................................. 10.0g Glucose ......................................................................................... 4.0g Yeast extract.................................................................................. 4.0g pH 7.0 ± 0.2 at 25°C

Use: For the isolation and cultivation of Actinomadura species, Actinopolyspora species, Excellospora species, and Microspora species.

Source: This medium is available as a premixed powder from Oxoid Unipath. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the enumeration of microorganisms in potable and fresh water samples.

Yeast Extract Agar Composition per liter: Agar ............................................................................................ 15.0g Peptone ......................................................................................... 9.5g Yeast extract.................................................................................. 7.0g Beef extract................................................................................... 5.0g NaCl.............................................................................................. 5.0g pH 7.0 ± 0.2 at 25°C

1928

Yeast Extract Agar

Use: For the cultivation of Aeromonas salmonicida.

Yeast Extract Agar Composition per liter: Agar ............................................................................................ 20.0g Yeast extract.................................................................................. 1.0g Buffer solution ...........................................................................2.0mL pH 6.0 ± 0.2 at 25°C

Buffer Solution: Composition per 400.0mL:

Yeast Extract Glucose Agar Composition per liter: Agar ............................................................................................ 15.0g Glucose ....................................................................................... 15.0g K2HPO4 ........................................................................................ 5.2g KH2PO4 ...................................................................................... 3.18g NH4Cl ......................................................................................... 0.54g Yeast extract.................................................................................. 0.5g MgSO4 ........................................................................................ 0.12g Trace elements solution .............................................................5.0mL pH 7.0 ± 0.2 at 25°C

Trace Elements Solution: Composition per liter:

Preparation of Buffer Solution: Add 40.0g of Na2HPO4 to 300.0mL of distilled/deionized water. Mix thoroughly. Add 60.0g of KH2PO4. Mix thoroughly. Adjust pH to 6.0.

ZnSO4·7H2O............................................................................. 0.287g CuSO4·5H2O............................................................................. 0.249g MnSO4·4H2O............................................................................ 0.223g Na2MoO4·2H2O........................................................................ 0.124g CaCl2·6H2O .............................................................................. 0.118g KJ.............................................................................................. 0.083g H3BO3 ......................................................................................... 0.03g

Preparation of Medium: Add components to distilled/deionized

Preparation of Trace Elements Solution: Add components to

water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes.

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

Use: For the identification of Histoplasma capsulatum, Blastomyces

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

KH2PO4 ....................................................................................... 60.0g Na2HPO4 ..................................................................................... 40.0g

dermatitidis, and Coccidioides immitis.

Yeast Extract Agar Composition per liter: Agar ............................................................................................ 15.0g Proteose peptone ......................................................................... 10.0g NaCl .............................................................................................. 5.0g Yeast extract.................................................................................. 3.0g

Use: For the cultivation of a variety of heterotrophic microorganisms.

Yeast Extract Agar for Schizosaccharomyces Composition per liter: Glucose ....................................................................................... 30.0g Agar ............................................................................................ 15.0g Yeast extract.................................................................................. 5.0g

Use: For the cultivation and maintenance of Schizosaccharomyces japonicus and Schizosaccharomyces pombe. Yeast Extract Beef Extract Medium See: YB Medium Yeast Extract Dextrose Calcium Carbonate Agar See: YDC Agar © 2010 by Taylor and Francis Group, LLC

Use: For the cultivation and maintenance of Bacillus licheniformis, Bacillus species, Clavibacter michiganense, Flavobacterium indologenes, Hafnia alvei, Pseudomonas fluorescens, and Serratia marcescens.

Yeast Extract Glucose Broth Composition per liter: Glucose ....................................................................................... 15.0g K2HPO4 ........................................................................................ 5.2g KH2PO4 ...................................................................................... 3.18g NH4Cl ......................................................................................... 0.54g Yeast extract.................................................................................. 0.5g MgSO4 ........................................................................................ 0.12g Trace elements solution .............................................................5.0mL pH 7.0 ± 0.2 at 25°C

Trace Elements Solution: Composition per liter: ZnSO4·7H2O............................................................................. 0.287g CuSO4·5H2O............................................................................. 0.249g MnSO4·4H2O............................................................................ 0.223g Na2MoO4·2H2O........................................................................ 0.124g CaCl2·6H2O .............................................................................. 0.118g KJ.............................................................................................. 0.083g H3BO3 ......................................................................................... 0.03g

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Yeast Extract Malt Extract Glucose Agar

1929

Use: For the cultivation and maintenance of Bacillus licheniformis, Bacil-

Source: This medium is available as a premixed powder from Hi-

lus species, Clavibacter michiganense, Flavobacterium indologenes, Hafnia alvei, Pseudomonas fluorescens, and Serratia marcescens.

Media.

Yeast Extract Glucose Calcium Carbonate Agar Composition per liter: CaCO3 ......................................................................................... 20.0g Glucose ....................................................................................... 20.0g Agar ............................................................................................ 15.0g Yeast extract................................................................................ 10.0g

Use: For the isolation and cultivation of Erwinia species. Yeast Extract Glucose Carbonate Medium See: YGC Medium Yeast Extract Glucose Carbonate Peptone Medium See: YGCP Medium Yeast Extract Glucose Citrate Medium See: YGC Medium Yeast Extract Glucose Citrate Medium with Cysteine See: YGC Medium with Cysteine

Yeast Extract Glucose Medium Composition per liter: Agar ............................................................................................ 15.0g Yeast extract................................................................................ 10.0g Glucose ....................................................................................... 10.0g

Yeast Extract Glycerol Medium Composition per liter: Agar ............................................................................................ 15.0g Yeast extract.................................................................................. 5.0g Glycerol ...................................................................................50.0mL

Use: For the cultivation and maintenance of Geodermatophilus obscurus subspecies utahensis.

Yeast Extract HiVeg Agar

Use: For the enumeration of microorganisms in potable and freshwater samples. A highly nutritive medium recommended for plate count of microorganisms in water.

Yeast Extract Malt Extract Agar See: ISP Medium 2

Yeast Extract Malt Extract Agar, Diluted 1/10 Composition per liter: Agar ............................................................................................ 20.0g Malt extract................................................................................... 1.0g Yeast extract.................................................................................. 0.4g Glucose ......................................................................................... 0.4g pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Actinomadura atramentaria, Microtetraspora africana, Parvopolyspora pallida, and Streptosporangium fragile.

Yeast Extract Malt Extract Glucose Agar Composition per liter: Agar ............................................................................................ 20.0g Glucose ....................................................................................... 10.0g Neopeptone ................................................................................... 5.0g Malt extract................................................................................... 3.0g Yeast extract.................................................................................. 3.0g

Use: For the isolation and cultivation of yeasts.

Yeast Extract Malt Extract Glucose Agar Composition per liter: Agar ............................................................................................ 20.0g Malt extract................................................................................. 10.0g Glucose ......................................................................................... 4.0g Yeast extract.................................................................................. 4.0g pH 7.2 ± 0.2 at 25°C

Composition per liter:

Use: For the cultivation and maintenance of Nocardia asteroides,

Agar ............................................................................................ 15.0g Plant peptone................................................................................. 5.0g Yeast extract.................................................................................. 3.0g pH 7.2 ± 0.2 at 25°C

Nocardia farcinica, Streptomyces antibioticus, Streptomyces argenteolus, Streptomyces aureofaciens, Streptomyces bluensis, Streptomyces caelestis, Streptomyces cinnamoneus, Streptomyces echinatus, Streptomyces griseocarneus, Streptomyces griseus, Streptomyces hawaiien-

1930

Yeast Extract Mannitol Agar

sis, Streptomyces kanamyceticus, Streptomyces kentuckensis, Streptomyces murinus, Streptomyces netropsis, Streptomyces niveus, Streptomyces nogalater, Streptomyces nousei, Streptomyces paucisporogenes, Streptomyces rimosus, Streptomyces sparsogenes, Streptomyces spectabilis, Streptomyces tendae, Streptomyces tenebraruis, Streptomyces violaceoruber, Streptomyces viridifaciens, and Vibrio salmonicida.

Yeast Extract Mannitol Agar Composition per liter: Agar ............................................................................................ 15.0g Mannitol...................................................................................... 10.0g CaCO3 ........................................................................................... 4.0g K2HPO4 ......................................................................................... 0.5g Yeast extract.................................................................................. 0.4g MgSO4·7H2O ................................................................................ 0.2g NaCl .............................................................................................. 0.1g pH 6.8–7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Omit CaCO3 if a clear solution is needed. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of members of the rhizobiaceae.

Yeast Extract Medium Composition per liter: Yeast extract................................................................................ 10.0g

Preparation of Medium: Add yeast extract to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Pseudomonas cepacia.

Yeast Extract Medium with Sodium Sulfide Composition per liter: Yeast extract................................................................................ 10.0g Na2S ............................................................................................ 0.15g

Preparation of Medium: Add yeast extract to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Immediately prior to inoculation, add 0.15g of Na2S. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Rhodospirillum molischianum.

Yeast Extract Mineral Agar Composition per liter: Agar ............................................................................................ 15.0g Yeast extract.................................................................................. 4.0g NaHPO4·12H2O ............................................................................ 3.5g K2HPO4 ......................................................................................... 1.0g NH4Cl ........................................................................................... 0.5g MgSO4·7H2O .............................................................................. 0.03g pH 7.0 ± 0.2 at 25°C

Use: For the cultivation of Bacillus azotoformans.

Yeast Extract Mineral Medium (DSMZ Medium 259) Composition per liter: Agar ............................................................................................ 15.0g Yeast extract.................................................................................. 4.0g Na2HPO4·12H2O........................................................................... 3.5g K2HPO4......................................................................................... 1.0g NH4Cl ........................................................................................... 0.5g MgSO4·7H2O .............................................................................. 0.03g pH 7.1 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Bacillus azotoformans. Yeast Extract Nutrient Agar Medium See: YNA Medium Yeast Extract Nutrient Gelatin Medium See: YNG Medium Yeast Extract Peptone Beef Extract Medium See: YEPB Medium

Yeast Extract Peptone Starch Agar Composition per liter: Agar ............................................................................................ 18.0g Soluble starch.............................................................................. 10.0g Peptone ....................................................................................... 10.0g CaCO3 ........................................................................................... 5.0g Sodium acetate.............................................................................. 5.0g Yeast extract.................................................................................. 3.0g KH2PO4......................................................................................... 0.5g K2HPO4......................................................................................... 0.5g MgSO4·7H2O ................................................................................ 0.3g Sodium citrate........................................................................... 0.027g NaCl............................................................................................ 0.01g MnSO4·5H2O .............................................................................. 0.01g CuSO4·5H2O .............................................................................. 1.0mg CoCl2·6H2O ............................................................................... 1.0mg FeSO4·7H2O............................................................................... 1.0mg

Preparation of Medium: Add components to tap water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation and maintenance of Bacillus species that utilize starch as a carbon source. Yeast Extract Peptone Sulfate Cysteine Medium See: YPSC Medium

Yeast Extract Phosphate Agar (YEP Agar)

Preparation of Medium: Add components to distilled/deionized

Composition per liter:

Agar ............................................................................................ 20.0g Yeast extract.................................................................................. 1.0g KH2PO4......................................................................................... 0.3g

Yeast Extract Sucrose Agar

Na2HPO4 ....................................................................................... 0.2g Phenol Red ................................................................................. 1.0mg

Use: For the isolation of dimorphic pathogenic fungi from clinical specimens. Yeast Extract Proteose Peptone Medium See: YEPP Medium

Yeast Extract Rose Bengal HiVeg Broth Base with Sorbose

1931

Pancreatic digest of casein.......................................................... 10.0g Yeast extract................................................................................ 10.0g Sodium lactate ............................................................................ 10.0g KH2PO4......................................................................................... 2.5g MnSO4 ....................................................................................... 5.0mg pH 7.0 ± 0.2 at 25°C

Use: For the isolation, cultivation, and maintenance of Propionibacterium species.

Yeast Extract Sucrose Agar (YESA) (ATCC Medium 2125)

Composition per liter:

Na2HPO4 ................................................................................... 17.25g Yeast extract.................................................................................. 5.0g Synthetic detergent........................................................................ 2.0g NaCl .............................................................................................. 1.0g Sodium pyruvate ........................................................................... 1.0g Rose Bengal ................................................................................ 0.04g MgSO4 ........................................................................................ 0.01g Sorbose solution.....................................................................100.0mL pH 7.9 ± 0.2 at 25°C

Sucrose........................................................................................ 20.0g Agar ............................................................................................ 15.0g Yeast extract................................................................................. .4.0g KH2PO4......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g pH 6.2 ± 0.2 at 25°C

Source: This medium, without sorbose solution, is available as a premixed powder from HiMedia.

Sorbose Solution: Composition per 100.0mL: Sorbose.......................................................................................... 4.0g

Preparation of Sorbose Solution: Add sorbose to distilled/deionized water and bring volume to 100.0mL. Filter sterilize.

Preparation of Medium: Add components, except sorbose solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45–-50°C. Aseptically add 100.0mL of sterile sorbose solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes. Use: For the cold enrichment for recovery of Yersinia enterocolitica and Yersinia pseudotuberculosis from foods.

Yeast Extract Skim Milk Agar Composition per liter: Agar ............................................................................................ 15.0g Skim milk powder....................................................................... 10.0g Yeast extract.................................................................................. 1.0g

Use: For the cultivation of Lysobacter enzymogenes.

Yeast Extract Sodium Lactate Medium Composition per liter: Agar ............................................................................................ 15.0g © 2010 by Taylor and Francis Group, LLC

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.2. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of various fungi.

Yeast Extract Sucrose Agar Composition per liter: Sucrose...................................................................................... 150.0g Agar ............................................................................................ 20.0g Yeast extract............................................................................... .20.0g MgSO4·7H2O ................................................................................ 0.5g Trace metals solution .................................................................1.0mL pH 6.5 ± 0.2 at 25°C

Trace Metals Solution: Composition per 100.0mL: ZnSO4·7H2O ................................................................................. 1.0g CuSO4·5H2O................................................................................. 0.5g

Preparation of Trace Metals Solution: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Use: For the cultivation and maintenance of various fungi. Yeast Extract Tryptone Medium See: YT Medium Yeast Extract Tryptone NaCl Medium See: YTN Medium

1932

Yeast Fermentation Broth

Yeast Fermentation Broth Composition per liter: Carbohydrate............................................................................... 10.0g Pancreatic digest of gelatin ........................................................... 7.5g Yeast extract.................................................................................. 5.5g Bromcresol Purple ................................................................... 16.0mg

Use: For fermentation tests of specific carbohydrates used in the characterization and identification of yeasts. Gas accumulation in the Durham tube and a color change of the medium to yellow indicates carbohydrate fermentation.

Yeast Fermentation HiVeg Broth Base with Carbohydrate (Bromcresol Purple HiVeg Broth Base) Composition per liter: Plant peptone............................................................................... 10.0g NaCl .............................................................................................. 5.0g Plant extract .................................................................................. 3.0g Bromcresol Purple ...................................................................... 0.04g Carbohydrate solution..............................................................50.0mL pH 7.0 ± 0.2 at 25°C

Source: This medium, without carbohydrate solution, is available as a premixed powder from HiMedia.

Carbohydrate Solution: Composition per 100.0mL: Carbohydrate............................................................................... 10.0g

Preparation of Carbohydrate Solution: Add carbohydrate to distilled/deionized water and bring volume to 100.0mL. Adonitol, arabinose, cellobiose, glucose, dulcitol, fructose, galactose, inositol, lactose, maltose, mannitol, raffinose, rhamnose, salicin, sorbitol, sucrose, trehalose, xylose, or other carbohydrates may be used. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except carbohydrate solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute in 10.0mL volumes into test tubes containing inverted Durham tubes. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Add 0.5mL of sterile carbohydrate solution to each tube. Use: For the determination of carbohydrate fermentation reactions of microorganisms.

Yeast Fermentation Medium Composition per liter: Peptone.......................................................................................... 7.5g Yeast extract.................................................................................. 4.5g Bromthymol Blue (1.6% solution).............................................1.0mL Carbohydrate solution................................................................1.0mL

Preparation of Carbohydrate Solution: Add carbohydrate to distilled/deionized water and bring volume to 10.0mL. Glucose, maltose, lactose, galactose, or trehalose may be used. If raffinose is used, prepare a 12% solution. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except carbohydrate solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute in 2.0mL volumes into test tubes that contain an inverted Durham tube. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 1.0mL of sterile carbohydrate solution. Mix thoroughly. Use: For the cultivation and differentiation of yeast based on carbohydrate fermentation patterns. Yeasts that can ferment a specific carbohydrate turn the medium yellow.

Yeast Glucose Agar Composition per liter: Agar ............................................................................................ 15.0g Pancreatic digest of gelatin......................................................... 7.75g Beef extract................................................................................. 4.75g Yeast extract.................................................................................. 2.5g K2HPO4......................................................................................... 2.5g Glucose ......................................................................................... 1.0g pH 7.0 ± 0.2 at 25°C

Use: For the cultivation and maintenance of a wide variety of bacteria.

Yeast Glucose Agar Composition per liter: Glucose ....................................................................................... 20.0g Agar ............................................................................................ 15.0g Yeast extract................................................................................ 10.0g pH 7.0 ± 0.2 at 25°C

Use: For the cultivation of Acetobacter aceti, Acetobacter hansenii, Acetobacter liquefaciens, Acetobacter pasteurianus, Acetobacter species, Brevibacterium species, Dermabacter hominus, Clostridium saccharoperbutylacetonicum, Corynebacterium amycolatum, Gluconobacter asaii, Gluconobacter cerinus, Gluconobacter frateurii, Gluconobacter oxydans, Gluconobacter species, Lactococcus piscium, and Streptococcus mutans.

Yeast Glucose Agar for Acetobacter Composition per liter: Glucose ..................................................................................... 100.0g CaCO3 ......................................................................................... 20.0g Agar ............................................................................................ 15.0g Yeast extract................................................................................ 10.0g pH 7.0 ± 0.2 at 25°C

Yeast Malate Medium

1933

Use: For the cultivation and maintenance of Acetobacter aceti, Acetobacter pasteurianus, and Gluconobacter oxydans.

Plant extract .................................................................................. 1.5g Glucose ......................................................................................... 1.0g pH 6.6 ± 0.05 at 25°C

Yeast Glucose Broth

Source: This medium is available as a premixed powder from Hi-

Composition per liter:

Media.

Pancreatic digest of gelatin ......................................................... 7.75g Beef extract ................................................................................. 4.75g Yeast extract.................................................................................. 2.5g K2HPO4 ......................................................................................... 2.5g Glucose ......................................................................................... 1.0g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Staphylococcus caseolyticus.

Yeast Glucose Broth

Use: For antibiotic assay testing.

Yeast Malate Medium Composition per liter: Yeast extract.................................................................................. 5.0g Sodium malate .............................................................................. 1.0g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

Composition per liter:

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Glucose ....................................................................................... 20.0g Yeast extract................................................................................ 10.0g

Use: For the cultivation of Rhodopseudomonas viridis.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Enterococcus mundtii, Enterococcus pseudoavium, Enterococcus raffinosus, Enterococcus solitarius, Enterococcus sulfureus, Lactococcus raffinolactis, and Vagococcus fluvialis.

Yeast Glucose Broth (YGB) Composition per liter: Beef extract ................................................................................. 10.0g Peptone........................................................................................ 10.0g NaCl .............................................................................................. 5.0g Glucose ......................................................................................... 5.0g Yeast extract.................................................................................. 3.0g pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation of Enterococcus faecalis, Streptococcus anginosus, and Rhodobacter sphaeroides. Yeast Glucose Litmus Milk See: YGLM Yeast Glucose Litmus Milk with Chalk See: YGLM with Chalk

Yeast Malate Medium Composition per liter: KH2PO4·12H2O ............................................................................ 1.0g NaHCO3 ........................................................................................ 1.0g Sodium malate .............................................................................. 1.0g (NH4)2SO4 .................................................................................... 0.5g Trace elements solution .............................................................1.0mL pH 6.8 ± 0.2 at 25°C

Trace Elements Solution: Composition per liter: H3BO3 ........................................................................................... 0.3g CoCl2·6H2O .................................................................................. 0.2g ZnSO4·7H2O ................................................................................. 0.1g MnCl2·4H2O ............................................................................... 0.03g Na2MoO4·2H2O .......................................................................... 0.03g NiCl2·6H2O................................................................................. 0.02g CuCl2·2H2O ................................................................................ 0.01g

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except trace elements solution, to distilled/deionized water and bring volume to 999.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 1.0mL of sterile trace elements solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Rhodopseudomonas viridis.

Yeast Malate Medium (LMG 176) Composition per 1001.0mL:

Yeast HiVeg Agar (Antibiotic HiVeg Assay Medium No. 4) Composition per liter: Agar ............................................................................................ 15.0g Plant peptone................................................................................. 6.0g Yeast extract.................................................................................. 3.0g © 2010 by Taylor and Francis Group, LLC

KH2PO4·12H2O ............................................................................ 1.0g NaHCO3 ........................................................................................ 1.0g Sodium malate .............................................................................. 1.0g Yeast extract.................................................................................. 1.0g (NH4)2SO4 .................................................................................... 0.5g Trace elements solution .............................................................1.0mL pH 6.8–7.0 at 25°C

1934

Yeast Malt Agar

Trace Elements Solution: Composition per liter:

ing the pH to 3.0–4.0 at 45°–55°C or by the addition of antibiotics at 45°–50°C or below. Pour into sterile Petri dishes or leave in tubes.

H3BO3 ........................................................................................... 0.3g CoCl2·6H2O .................................................................................. 0.2g ZnSO4·7H2O ................................................................................. 0.1g MnCl2·4H2O................................................................................ 0.03g Na2MoO4·2H2O .......................................................................... 0.03g NiCl2·6H2O ................................................................................. 0.02g CuCl2·2H2O ................................................................................ 0.01g

Use: For the cultivation of fungi, including yeasts, and other aciduric

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8–7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation of Rhodopseudomonas viridis.

Yeast Malt Agar Composition per liter: Agar ............................................................................................ 20.0g Malt extract ................................................................................. 10.0g Glucose ......................................................................................... 4.0g Yeast extract.................................................................................. 4.0g pH 7.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of actinomycetes, yeasts, and fungi.

Yeast Malt Agar for Arthrobacter viscosus Composition per liter: NaCl ............................................................................................ 10.0g Pancreatic digest of casein .......................................................... 10.0g Yeast extract.................................................................................. 5.0g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Arthrobacter viscosus and Escherichia coli.

Yeast Malt Extract Agar (YM Agar) Composition per liter: Agar ............................................................................................ 20.0g Glucose ....................................................................................... 10.0g Peptone.......................................................................................... 5.0g Yeast extract.................................................................................. 3.0g Malt extract ................................................................................... 3.0g pH 6.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. The medium may be rendered selective by adjust© 2010 by Taylor and Francis Group, LLC

microorganisms such as Actinoplanes species, Streptomyces species, Streptoverticillium species, and Nocardia species.

Yeast Malt Extract Broth (YM Broth) Composition per liter: Glucose ....................................................................................... 10.0g Peptone ......................................................................................... 5.0g Yeast extract.................................................................................. 3.0g Malt extract................................................................................... 3.0g pH 6.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems. Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. The medium may be rendered selective by adjusting the pH to 3.0–4.0 at 45°–55°C or by the addition of antibiotics at 45°–50°C or below.

Use: For the cultivation of yeasts, molds, and other aciduric microorganisms such as Actinoplanes species, Streptomyces species, Streptoverticillium species, and Nocardia species.

Yeast Malt Extract Broth with 0.5% Calcium Carbonate (YM Broth with 0.5% CaCO3)

Composition per liter:

Glucose ....................................................................................... 10.0g Peptone ......................................................................................... 5.0g CaCO3 ........................................................................................... 5.0g Yeast extract.................................................................................. 3.0g Malt extract................................................................................... 3.0g pH 6.2 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Dekkera anomala, Dekkera bruxellensis, Dekkera claussenii, and Dekkera lambica.

Yeast Malt Extract Broth with 2.0% Calcium Carbonate (YM Broth with 2.0% CaCO3)

Composition per liter:

CaCO3 ......................................................................................... 20.0g Glucose ....................................................................................... 10.0g Peptone ......................................................................................... 5.0g Yeast extract.................................................................................. 3.0g Malt extract................................................................................... 3.0g pH 6.2 ± 0.2 at 25°C

Yeast Malt Extract Catalase Agar

may be rendered selective by adjusting the pH to 3.0–4.0 at 45°–55°C or by the addition of antibiotics at 45°–50°C or below.

Use: For the cultivation and maintenance of Dekkera abstinens.

Yeast Malt Extract Broth with Glucose (YM Broth with Glucose) Composition per liter:

1935

Peptone ......................................................................................... 5.0g Yeast extract.................................................................................. 3.0g Malt extract................................................................................... 3.0g pH 6.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into flasks or tubes. Autoclave for 15 min at 15 psi pressure–121°C.

Glucose ..................................................................................... 290.0g Peptone.......................................................................................... 5.0g CaCO3 ........................................................................................... 5.0g Yeast extract.................................................................................. 3.0g Malt extract ................................................................................... 3.0g pH 6.2 ± 0.2 at 25°C

Use: For the cultivation of Chrysosporium fastidium, Chrysosporium

Preparation of Medium: Add components to distilled/deionized

Composition per liter:

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. The medium may be rendered selective by adjusting the pH to 3.0–4.0 at 45°–55°C or by the addition of antibiotics .

Use: For the cultivation of yeasts and filamentous fungi.

Yeast Malt Extract Broth with 1.0% Methanol (YM Broth with 1.0% Methanol) Composition per liter: Glucose ....................................................................................... 10.0g Peptone.......................................................................................... 5.0g Yeast extract.................................................................................. 3.0g Malt extract ................................................................................... 3.0g Methanol ..................................................................................10.0mL pH 6.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except methanol, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Filter sterilize the methanol. Aseptically add the sterile methanol to the cooled sterile basal medium. Mix thoroughly. Distribute into sterile flasks or tubes.

Use: For the cultivation and maintenance of Pichia angusta and Wickerhamiella domercqiae.

Yeast Malt Extract Broth with 18% Sodium Chloride (YM Broth with 18% NaCl) Composition per liter: NaCl .......................................................................................... 180.0g Glucose ....................................................................................... 10.0g Peptone.......................................................................................... 5.0g Yeast extract.................................................................................. 3.0g Malt extract ................................................................................... 3.0g pH 6.2 ± 0.2 at 25°C

xerophilum, Curvularia pallescens, Eupenicillium molle, and Talaromyces ucrainicus.

Yeast Malt Extract Broth with 70.0% Sucrose (YM Broth with 70.0% Sucrose) Sucrose...................................................................................... 700.0g Glucose ....................................................................................... 10.0g Peptone ......................................................................................... 5.0g Yeast extract.................................................................................. 3.0g Malt extract................................................................................... 3.0g pH 6.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into flasks or tubes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Aspergillus penicilloides.

Yeast Malt Extract Catalase Agar (YM Catalase Agar) Composition per liter: Agar ............................................................................................ 15.0g K2HPO4....................................................................................... 5.74g Malt extract................................................................................... 5.0g Yeast extract.................................................................................. 5.0g NH4H2PO4 .................................................................................. 1.15g Magnesium sulfate solution.....................................................10.0mL Catalase solution......................................................................10.0mL

Catalase Solution: Composition per 10.0mL: Catalase.................................................................................... 60.0mg

Preparation of Catalase Solution: Add catalase to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Magnesium Sulfate Solution: Composition per 10.0mL: MgSO4·7H2O ......................................................................... 205.0mg

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

of Magnesium Sulfate Solution: Add MgSO4·7H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Use: For the cultivation and maintenance of Zygosaccharomyces rouxii.

Preparation of Medium: Add components, except catalase and

Preparation of Medium: Add components to distilled/deionized

Yeast Malt Extract Broth with 40.0% Sucrose (YM Broth with 40.0% Sucrose) Composition per liter: Sucrose...................................................................................... 400.0g Glucose ....................................................................................... 10.0g © 2010 by Taylor and Francis Group, LLC

Preparation

magnesium sulfate solutions, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 50°C. Aseptically add filter-sterilized catalase and magnesium sulfate solutions.

Use: For the cultivation and maintenance of Rarobacter faecitabidus.

1936

Yeast Malt HiVeg Agar

Yeast Malt HiVeg Agar (YM HiVeg Agar) Agar ............................................................................................ 20.0g Glucose ....................................................................................... 10.0g Plant peptone................................................................................. 5.0g Malt extract ................................................................................... 3.0g Yeast extract.................................................................................. 3.0g pH 7.2 ± 0.2 at 25°C

NaCl............................................................................................ 0.25g Yeast extract................................................................................ 0.25g CaSO4·2H2O ............................................................................ 30.0mg FeSO4·7H2O............................................................................... 3.5mg H3BO3 ..................................................................................... 500.0μg MnSO4·4H2O .......................................................................... 400.0μg ZnSO4·7H2O ........................................................................... 160.0μg CuSO4·5H2O ............................................................................. 80.0μg pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi-

Preparation of Medium: Add components to distilled/deionized

Composition per liter:

Media.

Use: For the cultivation and maintenance of actinomycetes, yeasts, and fungi.

Yeast Malt HiVeg Broth (YM HiVeg Broth) Composition per liter: Glucose ....................................................................................... 10.0g Plant peptone................................................................................. 5.0g Malt extract ................................................................................... 3.0g Yeast extract.................................................................................. 3.0g pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from HiMedia.

Use: For the cultivation and maintenance of actinomycetes, yeasts, and fungi.

Use: For the cultivation of Bradyrhizobium japonicum and Rhizobium leguminosarum.

Yeast Mannitol Agar, Modified Composition per liter: Agar ............................................................................................ 15.0g Mannitol...................................................................................... 10.0g CaCO3 ........................................................................................... 4.0g K2HPO4......................................................................................... 0.5g Yeast extract.................................................................................. 0.4g MgSO4·7H2O ................................................................................ 0.2g NaCl.............................................................................................. 0.1g pH 6.8 ± 0.2 at 25°C

Use: For the cultivation of Amycolata autotrophica, Amycolatopsis orientalis, Nocardioides albus, Promicromonospora citrea, Rhizobium fredii, Rhizobium galegae, Rhizobium leguminosarum, Rhizobium loti, Rhizobium meliloti, and Rhizobium tropici.

Yeast Milk Medium Yeast Mannitol Agar Composition per liter: Agar ............................................................................................ 15.0g Mannitol...................................................................................... 10.0g K2HPO4 ......................................................................................... 0.5g Yeast extract.................................................................................. 0.4g MgSO4·7H2O ................................................................................ 0.2g NaCl .............................................................................................. 0.1g pH 6.8 ± 0.2 at 25°C

Use: For the cultivation of Rhizobium and Azorhizobium species.

Yeast Mannitol Agar Composition per liter: Agar ............................................................................................ 20.0g Mannitol...................................................................................... 10.0g Na2HPO4·12H2O........................................................................... 1.2g KH2PO4 ....................................................................................... 0.55g MgSO4·7H2O .............................................................................. 0.25g © 2010 by Taylor and Francis Group, LLC

Composition per liter: Agar ............................................................................................ 15.0g Skim milk.................................................................................... 10.0g Yeast extract.................................................................................. 1.0g

Use: For the cultivation and maintenance of Lysobacter enzymogenes.

Yeast Morphology Agar Composition per liter: (NH4)2SO4 .................................................................................. 32.5g Agar ............................................................................................ 18.0g Glucose ....................................................................................... 10.0g Asparagine .................................................................................... 1.5g KH2PO4......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g NaCl.............................................................................................. 0.5g CaCl2·2H2O .................................................................................. 0.1g DL-Methionine............................................................................. 0.02g DL-Tryptophan............................................................................. 0.02g

Yeast Nitrogen Base with Carbohydrate

L-Histidine·HCl ........................................................................... 0.01g

Inositol ....................................................................................... 2.0mg H3BO3 ........................................................................................ 0.5mg Calcium pantothenate ................................................................ 0.4mg MgSO4·7H2O ............................................................................. 0.4mg Niacin......................................................................................... 0.4mg Pyridoxine·HCl .......................................................................... 0.4mg Thiamine·HCl ............................................................................ 0.4mg ZnSO4·7H2O .............................................................................. 0.4mg p-Aminobenzoic acid................................................................. 0.2mg FeCl3 .......................................................................................... 0.2mg Riboflavin .................................................................................. 0.2mg Na2MoO4·4H2O ......................................................................... 0.2mg KI ............................................................................................... 0.1mg CuSO4·5H2O ............................................................................ 0.04mg Biotin ..........................................................................................2.0μg Folic acid.....................................................................................2.0μg pH 5.6 ± 0.2 at 25°C

Use: For agar dilution and diffusion disk testing with 5-flucytosine. For the microbiological assay of flucytosine using Candida kefyr ATCC 28838 or Saccharomyces cerevisiae ATCC 36375 as the indicator microorganism.

Yeast Nitrogen Base Composition per liter: (NH4)2SO4 ..................................................................................... 5.0g KH2PO4 ......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g NaCl .............................................................................................. 0.1g CaCl2·2H2O................................................................................... 0.1g DL-Methionine............................................................................. 0.02g DL-Tryptophan............................................................................. 0.02g L-Histidine·HCl ........................................................................... 0.01g Inositol ....................................................................................... 2.0mg KI ............................................................................................... 1.0mg H3BO3 ........................................................................................ 0.5mg ZnSO4·7H2O .............................................................................. 0.4mg MnSO4·4H2O ............................................................................. 0.4mg Thiamine·HCl ............................................................................ 0.4mg Pyroxidine·HCl .......................................................................... 0.4mg Niacin......................................................................................... 0.4mg Calcium pantothenate ................................................................ 0.4mg p-Aminobenzoic acid................................................................. 0.2mg Riboflavin .................................................................................. 0.2mg FeCl3 .......................................................................................... 0.2mg Na2MoO4·4H2O ......................................................................... 0.2mg CuSO4·5H2O ............................................................................ 0.04mg Folic acid.....................................................................................2.0μg Biotin ..........................................................................................2.0μg pH 5.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

1937

or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Alternately for carbon assimilation tests, prepare a 10X concentrated solution by adding components to distilled/deionized water and bringing volume to 100.0mL. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Prepare a carbohydrate solution by adding 0.5g of carbohydrate to 90.0mL of distilled/deionized water. Mix thoroughly. Filter sterilize. Aseptically add 0.5mL of the 10X concentrated solution to 4.5mL of the filter-sterilized carbohydrate solution. Mix thoroughly.

Use: For carbohydrate assimilation tests in the characterization and identification of yeasts.

Yeast Nitrogen Base, 10X with Asparagine and Glucose Composition per liter: Glucose ....................................................................................... 10.0g (NH4)2SO4 .................................................................................... 5.0g L-Asparagine ................................................................................. 1.5g KH2PO4......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g NaCl.............................................................................................. 0.1g CaCl2·2H2O .................................................................................. 0.1g DL-Methionine............................................................................. 0.02g DL-Tryptophan............................................................................. 0.02g L-Histidine·HCl ........................................................................... 0.01g Inositol ....................................................................................... 2.0mg H3BO3 ........................................................................................ 0.5mg ZnSO4·7H2O .............................................................................. 0.4mg MnSO4·4H2O ............................................................................. 0.4mg Thiamine·HCl ............................................................................ 0.4mg Pyridoxine·HCl .......................................................................... 0.4mg Niacin......................................................................................... 0.4mg Calcium pantothenate ................................................................ 0.4mg p-Aminobenzoic acid................................................................. 0.2mg Riboflavin .................................................................................. 0.2mg FeCl3 .......................................................................................... 0.2mg Na2MoO4·4H2O ......................................................................... 0.2mg KI ............................................................................................... 0.1mg CuSO4·5H2O............................................................................ 0.04mg Folic acid .................................................................................... 2.0μg Biotin .......................................................................................... 2.0μg pH 5.6 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Dilute 100.0mL of 10X medium with 900.0mL of distilled/deionized water. Mix thoroughly. Filter sterilize. Aseptically distribute into sterile tubes or flasks.

Use: For susceptibility tests with yeasts and fungi.

Yeast Nitrogen Base with Carbohydrate Composition per liter: Carbohydrate................................................................................. 5.0g (NH4)2SO4 .................................................................................... 5.0g KH2PO4......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g NaCl.............................................................................................. 0.1g CaCl2·2H2O .................................................................................. 0.1g DL-Methionine............................................................................. 0.02g DL-Tryptophan............................................................................. 0.02g L-Histidine·HCl ........................................................................... 0.01g Inositol ....................................................................................... 2.0mg

1938

Yeast Nitrogen Base Glucose Broth

KI ............................................................................................... 0.1mg H3BO3 ........................................................................................ 0.5mg ZnSO4·7H2O .............................................................................. 0.4mg MnSO4·4H2O ............................................................................. 0.4mg Thiamine·HCl ............................................................................ 0.4mg Pyroxidine·HCl .......................................................................... 0.4mg Niacin......................................................................................... 0.4mg Calcium pantothenate ................................................................ 0.4mg p-Aminobenzoic acid ................................................................. 0.2mg Riboflavin .................................................................................. 0.2mg FeCl3 .......................................................................................... 0.2mg Na2MoO4·4H2O ......................................................................... 0.2mg CuSO4·5H2O ............................................................................ 0.04mg Folic acid.....................................................................................2.0μg Biotin ..........................................................................................2.0μg pH 5.6 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize. Aseptically distribute into tubes or flasks.

Use: For carbohydrate assimilation tests in the characterization and identification of yeasts.

Yeast Nitrogen Base Glucose Broth Composition per liter: Yeast nitrogen base ..................................................................25.0mL Glucose solution ......................................................................25.0mL pH 5.6 ± 0.2 at 25°C

Yeast Nitrogen Base: Composition per 500.0mL:

Glucose Solution: Composition per 500.0mL: Glucose ....................................................................................... 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 500.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Aseptically combine 25.0mL of sterile yeast nitrogen base and 25.0mL of sterile glucose solution. Mix thoroughly.

Use: For the cultivation and enrichment of yeast from sewage and polluted waters.

Yeast Peptone Agar (ATCC Medium 1858) Composition per liter: Agar ............................................................................................ 15.0g Yeast extract.................................................................................. 2.5g Peptone ......................................................................................... 2.5g pH 7.0 ± 0.4 at 25°C

Use: For the cultivation of Cytophaga lytica, Pseudomonas lanceolata, Rhodobacter capsulatus, Rhodobacter sphaeroides, Rhodopseudomonas blastica, Rhodopseudomonas palustris, Rhodospirillum rubrum, Rubrivivax gelatinosus, Sphaerotilus natans, and Zoogloea ramigera.

Yeast Peptone Broth

(NH4)2SO4 ..................................................................................... 5.0g KH2PO4 ......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g NaCl .............................................................................................. 0.1g CaCl2·2H2O................................................................................... 0.1g DL-Methionine............................................................................. 0.02g DL-Tryptophan............................................................................. 0.02g L-Histidine·HCl ........................................................................... 0.01g Inositol ....................................................................................... 2.0mg H3BO3 ........................................................................................ 0.5mg ZnSO4·7H2O .............................................................................. 0.4mg MnSO4·4H2O ............................................................................. 0.4mg Thiamine·HCl ............................................................................ 0.4mg Pyroxidine·HCl .......................................................................... 0.4mg Niacin......................................................................................... 0.4mg Calcium pantothenate ................................................................ 0.4mg p-Aminobenzoic acid ................................................................. 0.2mg Riboflavin .................................................................................. 0.2mg FeCl3 .......................................................................................... 0.2mg Na2MoO4·4H2O ......................................................................... 0.2mg KI ............................................................................................... 0.1mg CuSO4·5H2O ............................................................................ 0.04mg Folic acid.....................................................................................2.0μg Biotin ..........................................................................................2.0μg

Composition per liter:

Source: Yeast nitrogen base is available as a premixed powder from BD Diagnostic Systems.

Composition per liter:

Yeast extract.................................................................................. 2.5g Peptone ......................................................................................... 2.5g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Rhodopseudomonas species.

Yeast Peptone Salt Medium Composition per liter: Agar ............................................................................................ 15.0g Peptone ......................................................................................... 2.5g Yeast extract.................................................................................. 2.5g NaCl............................................................................................ 1.25g pH 7.0 ± 0.4 at 25°C

Use: For the cultivation of Deinobacter grandis.

Yeast Peptone Succinate Medium (DSMZ Medium 988) Yeast extract ................................................................................. 3.0g Peptone ........................................................................................ 3.0g Sodium succinate ......................................................................... 2.3g pH 7.2 ± 0.2 at 25°C

Yeast Synthetic Minimal Medium

1939

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation of Sphingomonas aurantiaca and other Sphingomonas spp.

Use: For the cultivation of Streptomyces spp. For the cultivation and maintenance of Actinomadura livida, numerous Actinoplanes species, Actinosynnema mirum, Amycolata hydrocarbonoxydans, Amycolatopsis mediterranei, Amycolatopsis orientalis, Catellatospora ferruginea, Dactylosporangium aurantiacum, Microbispora chromogenes, Microbispora thermodiastatica, Micromonospora chalcea, Microtetraspora angiospora, Microtetraspora incanescens, Microtetraspora niveoalba, Microtetraspora spiralis, Nocardia nitrifians, Planobispora rosea, Planomonospora venezuelensis, Saccharothrix aerocolonigenes, Saccharothrix mutabilis, Sporichthya polymorpha, most Streptomyces species, many Streptosporangium species, and Trichotomospora caesia.

Yeast Starch Agar Composition per liter: Agar ............................................................................................ 15.0g Starch, soluble............................................................................. 15.0g Yeast extract.................................................................................. 4.0g K2HPO4 ......................................................................................... 0.5g MgSO4·7H2O ................................................................................ 0.5g pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Yeast Synthetic Medium with 5´-dTMP Composition per liter:

Use: For the cultivation of Streptomyces albospinus, Streptomyces longwoodensis, Streptomyces cystargineus, and Promicromonospora sukumoe.

Yeast Starch Agar, Diluted 1/10

Preparation of Medium: Add glucose, yeast nitrogen base without

Composition per liter: Agar ............................................................................................ 15.0g Soluble starch................................................................................ 1.0g Yeast extract.................................................................................. 0.2g pH 7.3 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Catellatospora citrea, Spirillospora species, and Streptosporangium viridialbum.

Yeast Starch Agar, Half Strength Composition per liter: Agar ............................................................................................ 15.0g Soluble starch................................................................................ 5.0g Yeast extract.................................................................................. 1.0g pH 7.3 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Microtetraspora ferruginea and Streptomyces sporoverrucocus.

Yeast Starch Agar A (DSMZ Medium 1027) Composition per liter: Agar ............................................................................................ 15.0g Starch, soluble............................................................................. 10.0g Yeast extract.................................................................................. 2.0g pH 7.3 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

amino acids, casamino acids without vitamins, and 2´-deoxythymidine-5´-monophosphate to distilled/deionized water and bring volume to 200.0mL. Mix thoroughly. Filter sterilize. Add agar to distilled/deionized water and bring volume to 800.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 50°C. Aseptically combine the two sterile solutions. Mix thoroughly. Pour into sterile Petri dishes or aseptically distribute into sterile tubes.

Use: For the cultivation and maintenance of Saccharomyces cerevisiae.

Yeast Synthetic Minimal Medium Composition per liter: D-Glucose .................................................................................... 20.0g

Agar ............................................................................................ 15.0g (NH4)2SO4 .................................................................................... 5.0g KH2PO4......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g NaCl.............................................................................................. 0.1g CaCl2·2H2O .................................................................................. 0.1g Inositol ....................................................................................... 2.0mg H3BO3 ........................................................................................ 0.5mg ZnSO4·7H2O .............................................................................. 0.4mg MnSO4·4H2O ............................................................................. 0.4mg Thiamine·HCl ............................................................................ 0.4mg Pyridoxine·HCl .......................................................................... 0.4mg Niacin......................................................................................... 0.4mg Calcium pantothenate ................................................................ 0.4mg p-Aminobenzoic acid................................................................. 0.2mg Riboflavin .................................................................................. 0.2mg FeCl3 .......................................................................................... 0.2mg Na2MoO4·4H2O ......................................................................... 0.2mg KI ............................................................................................... 0.1mg CuSO4·5H2O............................................................................ 0.04mg

1940

Yeast Synthetic Minimal Medium

Preparation of Medium: Add agar to 900.0mL of distilled/deionized water. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. In a separate flask, add remaining components to 100.0mL of distilled/deionized water. Mix thoroughly. Filter sterilize. Aseptically combine the two sterile solutions. Mix thoroughly. Pour into sterile Petri dishes. Use: For the cultivation of a wide variety of heterotrophic microorganisms.

Pancreatic digest of casein............................................................ 5.0g KH2PO4......................................................................................... 2.0g CaCl2·2H2O .................................................................................. 0.5g MnCl2·4H2O ................................................................................. 0.5g

Use: For the cultivation and maintenance of Bacillus circulans.

Yeast Water Agar Composition per liter:

Yeast Synthetic Minimal Medium Composition per liter: Glucose ....................................................................................... 20.0g Agar ............................................................................................ 15.0g Yeast nitrogen base without amino acids...................................... 6.7g

Preparation of Medium: Add glucose and yeast nitrogen base without amino acids to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Add agar to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically combine the two sterile solutions. Mix thoroughly. Pour into sterile Petri dishes or aseptically distribute into sterile tubes.

Glucose ....................................................................................... 20.0g Agar ............................................................................................ 15.0g Casein hydrolysate........................................................................ 5.0g Yeast extract.................................................................................. 4.0g KH2PO4....................................................................................... 0.55g KCl................................................................................................ 0.4g CaCl2 ........................................................................................... 0.13g MgCl2·7H2O ............................................................................... 0.13g FeCl3·6H2O ................................................................................ 2.5mg MnSO4·4H2O ............................................................................. 2.5mg Bromcresol Green solution ........................................................1.0mL

Bromcresol Green Solution: Composition per 10.0mL:

Use: For the cultivation and maintenance of Pichia angusta and Sac-

Bromcresol Green....................................................................... 0.22g Ethanol.....................................................................................10.0mL

charomyces cerevisiae.

Preparation of Bromcresol Green Solution: Add Bromcresol Green to 10.0mL of ethanol. Mix thoroughly. Filter sterilize.

Yeast Tryptone Medium Composition per liter: Pancreatic digest of casein .......................................................... 10.0g NaCl ............................................................................................ 10.0g Yeast extract.................................................................................. 5.0g pH 7.0 ± 0.2 at 25°C

Use: For the cultivation of Zymomonas species.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Escherichia coli.

Yeast Tryptone Medium with Streptomycin Composition per liter: Pancreatic digest of casein .......................................................... 10.0g NaCl ............................................................................................ 10.0g Yeast extract.................................................................................. 5.0g Streptomycin ................................................................................. 0.2g pH 7.0 ± 0.2 at 25°C

Yeastrel Agar Composition per liter: Agar ............................................................................................ 15.0g Peptone ......................................................................................... 9.5g Yeastrel ......................................................................................... 7.0g Lab-lemco (meat extract).............................................................. 5.0g NaCl.............................................................................................. 5.0g pH 7.0 ± 0.2 at 25°C

Source: Lab-lemco is available from Oxoid Unipath. Yeastrel is produced by Mapleton’s Foods Ltd., Moss Street, Liverpool and is available from health food shops. Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Escherichia coli.

Use: For the cultivation of Aeromonas salmonicida.

Preparation of Medium: Add components to distilled/deionized

Yeast Tryptone Starch Medium Composition per liter: Agar ............................................................................................ 15.0g Soluble starch.............................................................................. 10.0g Yeast extract.................................................................................. 5.0g © 2010 by Taylor and Francis Group, LLC

YED Medium, Salted (DSMZ Medium 1123) Composition per liter: Agar ........................................................................................... 20.0g NaCl ........................................................................................... 10.0g

YEPD-FA Medium

1941

Glucose ........................................................................................ 7.0g Yeast extract ................................................................................. 4.0g pH 7.0 ± 0.2 at 25°C

Peptone ....................................................................................... 20.0g Yeast extract................................................................................ 10.0g

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Martelella mediterranea.

YEME Thiostrepton Medium Composition per liter: Sucrose...................................................................................... 340.0g Peptone.......................................................................................... 5.0g Malt extract ................................................................................... 3.0g Yeast extract.................................................................................. 3.0g Thiostrepton solution ...............................................................10.0mL MgCl2·6H2O solution.................................................................2.0mL

Thiostrepton Solution: Composition per 10.0mL: Thiostrepton ............................................................................. 10.0mg

Preparation of Thiostrepton Solution: Add thiostrepton to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components to distilled/deionized

Use: For the cultivation and maintenance of Saccharomyces cerevisiae and Yarrowia lipolytica.

YEPB Medium (Yeast Extract Peptone Beef Extract Medium) Composition per liter: Beef extract................................................................................. 10.0g Polypeptone™ ............................................................................ 10.0g Yeast extract.................................................................................. 5.0g NaCl.............................................................................................. 3.0g MnCl2·4H2O ................................................................................. 0.1g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0 with KOH. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Adjust pH to 7.0 with KOH.

Use: For the cultivation of Microbacterium species.

MgCl2·6H2O Solution:

Composition per 100.0mL: MgCl2·6H2O................................................................................ 50.8g

Preparation of MgCl2·6H2O Solution: Add MgCl2·6H2O to dis-

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except thiostrepton solution and MgCl2·6H2O solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 10.0mL of sterile thiostrepton solution and 2.0mL of sterile solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Streptomyces lividans. YEP Agar See: Yeast Extract Phosphate Agar

YEP Galactose Agar Composition per liter: Agar ............................................................................................ 20.0g Galactose..................................................................................... 20.0g Peptone........................................................................................ 20.0g Yeast extract................................................................................ 10.0g

Use: For the cultivation of a variety of heterotrophic microorganisms.

YEP Galactose Medium Composition per liter: Agar ............................................................................................ 20.0g Galactose..................................................................................... 20.0g © 2010 by Taylor and Francis Group, LLC

YEPD Agar Composition per liter: Glucose ....................................................................................... 20.0g Mycological peptone .................................................................. 20.0g Agar ............................................................................................ 15.0g Yeast extract................................................................................ 10.0g pH 5.5 ± 0.2 at 25°C

Use: For the cultivation and maintenance of a variety of yeasts including Botryozyma nematodophila, Bullera crocea, Candida species, Cryptococcus neoformans, Hanseniaspora uvarum, Kluyveromyces lactis, Kluyveromyces marxianus, Metschnikowia pulcherrima, Phaffia rhodozyma, Pichia species, Rhodosporidium toruloides, Rhodotorula graminis, Saccharomyces species, Schizosaccharomyces pombe, Schwanniomyces occidentalis, Trichosporon species, Yamadazyma stipitis, Yarrowia lipolytica, Zygosaccharomyces bailii, and Zygosaccharomyces rouxii.

YEPD-FA Medium Composition per liter: Agar ............................................................................................ 20.0g Glucose ....................................................................................... 20.0g Peptone ....................................................................................... 20.0g Yeast extract................................................................................ 10.0g Tween™ 40................................................................................. 10.0g KH2PO4......................................................................................... 5.0g K2HPO4......................................................................................... 5.0g Myristic acid ........................................................................... 70.0mg Palmitic acid ............................................................................ 70.0mg Stearic acid............................................................................... 70.0mg

1942

YEPD Inositol Agar

Hemin Stock Solution: Composition per 10.0mL:

Use: For the cultivation and maintenance of Saccharomyces cerevi-

NaOH.......................................................................................... 0.04g Hemin chloride ........................................................................ 65.0mg Ethanol (50% solution) ..........................................................100.0mL

siae.

Preparation of Hemin Stock Solution: Combine components and mix thoroughly.

YEPD Inositol Agar Composition per liter: Glucose ....................................................................................... 20.0g Mycological peptone................................................................... 20.0g Agar ............................................................................................ 15.0g Yeast extract................................................................................ 10.0g Inositol ................................................................................... 180.0mg

Use: For the cultivation and maintenance of Saccharomyces cerevisiae.

YEPD Medium Composition per liter: Agar ............................................................................................ 20.0g Glucose ....................................................................................... 20.0g Peptone........................................................................................ 20.0g Yeast extract................................................................................ 10.0g

Use: For the cultivation of a variety of heterotrophic microorganisms.

YEPD Medium

Use: For the cultivation and maintenance of Saccharomyces cerevisiae.

YEPD Medium with 0.3M Sodium Chloride Composition per liter: Agar ............................................................................................ 20.0g Glucose ....................................................................................... 20.0g Peptone ....................................................................................... 20.0g NaCl.......................................................................................... 17.53g Yeast extract................................................................................ 10.0g

Use: For the cultivation and maintenance of Debaryomyces hansenii.

YEPG Medium Composition per liter: Glucose ......................................................................................... 1.0g Polypeptone™ .............................................................................. 2.0g NH4NO3 ........................................................................................ 0.2g Yeast extract.................................................................................. 0.2g pH 7.0 ± 0.2 at 25°C

Composition per liter:

Preparation of Medium: Add components to distilled/deionized

Peptone........................................................................................ 20.0g Yeast extract................................................................................ 10.0g

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation and maintenance of a variety of yeasts, including Botryozyma nematodophila, Bullera crocea, Candida species, Cryptococcus neoformans, Hanseniaspora uvarum, Kluyveromyces lactis, Kluyveromyces marxianus, Metschnikowia pulcherrima, Phaffia rhodozyma, Pichia species, Rhodosporidium toruloides, Rhodotorula graminis, Saccharomyces species, Schizosaccharomyces pombe, Schwanniomyces occidentalis, Trichosporon species, Yamadazyma stipitis, Yarrowia lipolytica, Zygosaccharomyces bailii, and Zygosaccharomyces rouxii.

YEPD Medium with Heme Composition per liter: Agar ............................................................................................ 20.0g Glucose ....................................................................................... 20.0g Peptone........................................................................................ 20.0g Yeast extract................................................................................ 10.0g Hemin stock solution ...............................................................20.0mL © 2010 by Taylor and Francis Group, LLC

Use: For the cultivation of Pseudomonas putida and Pseudomonas fluorescens. YEPG with 0.5% CaCO3 See: Bacillus racemilacticus Agar

YEPP Medium (Yeast Extract Proteose Peptone Medium) Composition per liter: Agar ............................................................................................ 15.0g Proteose peptone......................................................................... 10.0g NaCl.............................................................................................. 5.0g Yeast extract.................................................................................. 3.0g pH 7.2–7.4 ± 0.2 at 25°C

Use: For the cultivation and maintenance of Pseudomonas species.

YGC Medium

1943

Yersinia Isolation Agar See: CAL Agar

psi pressure–121°C. Cool to 50°C. Aseptically add selective supplement. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Yersinia Isolation HiVeg Agar

Use: For the isolation and enumeration of Yersinia enterocolitica from food and clinical specimens.

Composition per liter: Agar ............................................................................................ 15.0g Plant peptone............................................................................... 15.0g Lactose ........................................................................................ 10.0g Sodium citrate ............................................................................. 10.0g Plant extract No. 1 ........................................................................ 8.5g Na2S2O3 ........................................................................................ 8.5g Yeast extract.................................................................................. 5.0g Synthetic detergent No. II ............................................................. 3.0g Synthetic detergent No. III............................................................ 2.0g CaCl2 ............................................................................................. 1.0g Ferric citrate .................................................................................. 1.0g Neutral Red ............................................................................... 0.025g Brilliant Green ........................................................................... 0.3mg pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

Yersinia Selective HiVeg Agar Base with Selective Supplement Composition per liter: Plant special peptone .................................................................. 20.0g Mannitol...................................................................................... 20.0g Agar ............................................................................................ 12.5g Sodium pyruvate........................................................................... 2.0g Yeast extract.................................................................................. 2.0g NaCl.............................................................................................. 1.0g Synthetic detergent No. III ........................................................... 0.5g Neutral Red................................................................................. 0.03g MgSO4 ........................................................................................ 0.01g Crystal Violet ............................................................................. 1.0mg Selective supplement .................................................................6.0mL pH 7.4 ± 0.2 at 25°C

water and bring volume to 1.0L. Mix thoroughly. Heat to boiling. Do not autoclave. Pour into sterile Petri dishes.

Source: This medium, without selective supplement, is available as a

Use: For the isolation and characterization of Yersinia enterocolitica

Selective Supplement: Composition per 6.0mL:

from fecal specimens and enumeration of Yersinia enterocolitica from water and other liquid specimens.

Yersinia Selective Agar See: CIN Agar

Yersinia Selective Agar Base Composition per liter: Mannitol...................................................................................... 20.0g Peptone........................................................................................ 17.0g Agar ............................................................................................ 12.5g Proteose peptone ........................................................................... 3.0g Yeast extract.................................................................................. 2.0g Sodium pyruvate ........................................................................... 2.0g NaCl .............................................................................................. 1.0g Sodium desoxycholate .................................................................. 0.5g MgSO4·7H2O .............................................................................. 0.01g Neutral Red ................................................................................. 0.03g Crystal Violet ............................................................................. 1.0mg Selective supplement .................................................................6.0mL pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems and Oxoid Unipath. Selective Supplement: Composition per 6.0mL: Cefsulodin ................................................................................ 15.0mg Irgasan........................................................................................ 4.0mg Novobiocin................................................................................. 2.5mg Ethanol .......................................................................................2.0mL

premixed powder from HiMedia.

Cefsulodin................................................................................ 15.0mg Irgasan........................................................................................ 4.0mg Novobiocin ................................................................................ 2.5mg Ethanol.......................................................................................2.0mL

Preparation of Selective Supplement: Aseptically add components to 4.0mL of distilled/deionized water and 2.0mL of ethanol. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add selective supplement. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation and enumeration of Yersinia enterocolitica from food and clinical specimens. YGB See: Yeast Glucose Broth

YGC Medium (Yeast Extract Glucose Carbonate Medium) (ATCC Medium 73) Composition per liter: Agar ............................................................................................ 20.0g Glucose ....................................................................................... 20.0g CaCO3 ......................................................................................... 20.0g Yeast extract................................................................................ 10.0g

Preparation of Medium: Add components to distilled/deionized

nents to 4.0mL of distilled/deionized water and 2.0mL of ethanol. Mix thoroughly.

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 30 min at 10 psi pressure–115°C. Cool to 48°C. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Preparation of Medium: Add components to distilled/deionized

Use: For the cultivation of Xanthomonas species, Erwinia species, Kluy-

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

vera species, Rhodococcus species, Streptomyces species, Pseudomonas pseudoalcaligenes, and Xylophilus ampelinus.

Preparation of Selective Supplement: Aseptically add compo-

1944

YGC Medium

YGC Medium (Yeast Extract Glucose Carbonate Medium) (ATCC Medium 459) Composition per liter: Glucose ....................................................................................... 50.0g Agar ............................................................................................ 15.0g CaCO3 ......................................................................................... 12.5g Yeast extract.................................................................................. 5.0g

Use: For the cultivation and maintenance of Acetobacter species.

YGC Medium (Yeast Extract Glucose Citrate Medium) (ATCC Medium 216) Beef extract ................................................................................. 10.0g Glucose ....................................................................................... 10.0g Peptone........................................................................................ 10.0g Ammonium citrate ........................................................................ 5.0g Yeast extract.................................................................................. 5.0g Sodium acetate .............................................................................. 2.0g Tween™ 80 ................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.2g MnSO4·4H2O .............................................................................. 0.05g pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of Leuconostoc species.

YGC Medium with Cysteine (Yeast Extract Glucose Citrate Medium with Cysteine)

des.

YGC Medium with Glutamic Acid (Yeast Extract Glucose Carbonate Medium with Glutamic Acid) Composition per liter: Agar ............................................................................................ 20.0g Glucose ....................................................................................... 20.0g CaCO3 ......................................................................................... 20.0g Agar ............................................................................................ 20.0g Yeast extract................................................................................ 10.0g Glutamic acid................................................................................ 0.1g water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 30 min at 10 psi pressure–115°C. Cool to 48°C. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Xanthomonas campestris.

YGCB Salt Medium Composition per liter: NaCl............................................................................................ 50.0g Beef broth ................................................................................... 10.0g Glucose ....................................................................................... 10.0g Peptone ....................................................................................... 10,0g Triammonium citrate .................................................................... 5.0g Yeast extract.................................................................................. 5.0g Sodium acetate.............................................................................. 2.0g MgSO4·7H2O ................................................................................ 0.2g MnSO4·4H2O ........................................................................... 50.0mg Tween™ 80................................................................................1.0mL pH 6.7 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Composition per liter: Glucose ....................................................................................... 10.0g Peptone........................................................................................ 10.0g Beef extract ................................................................................. 10.0g Yeast extract.................................................................................. 5.0g Ammonium citrate ........................................................................ 5.0g Sodium acetate .............................................................................. 2.0g Tween™ 80 ................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.2g MnSO4·4H2O .............................................................................. 0.05g L-Cysteine·HCl·H2O solution ..................................................10.0mL pH 6.5 ± 0.2 at 25°C L-Cysteine·HCl

Solution: Composition per 10.0mL:

L-Cysteine·HCl·H2O ..................................................................... 0.5g L-cyste-

ine·HCl·H2O to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Use: For the cultivation and maintenance of Leuconostoc mesenteroi-

Preparation of Medium: Add components to distilled/deionized

Composition per liter:

Preparation of L-Cysteine·HCl Solution: Add

990.0mL. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add L-cysteine hydrochloride solution.

Use: For the cultivation of Tetragenococcus halophila.

YGCP Medium (Yeast Extract Glucose Carbonate Peptone Medium) Composition per liter: Glucose ....................................................................................... 20.0g Agar ............................................................................................ 17.5g CaCO3 ......................................................................................... 10.0g Yeast extract.................................................................................. 2.5g Peptone ......................................................................................... 2.5g NaCl.............................................................................................. 1.0g K2HPO4......................................................................................... 1.0g MgSO4 .......................................................................................... 0.5g

Preparation of Medium: Add components, except calcium carbonate, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.2. Add calcium carbonate. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

YI-S Medium

1945

Use: For the cultivation and maintenance of Xanthomonas campestris and Xanthomonas oryzae.

MgSO4·7H2O................................................................................ 0.2g MnSO4·4H2O.............................................................................. 0.05g pH 6.8 ± 0.2 at 25°C

YGLM (Yeast Glucose Litmus Milk)

Preparation of Medium: Add components to distilled/deionized

Composition per liter: Glucose ....................................................................................... 10.0g Skim milk powder......................................................................... 8.0g Yeast extract.................................................................................. 3.0g Litmus ......................................................................................... 0.75g

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation of Carnobacterium divergens, Carnobacterium piscicola, Lactobacillus delbrueckii, Lactobacillus fermentum, Lactococcus lactis, Streptococcus ferus, and Streptococcus sobrinus.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly for 15–20 min. Distribute into tubes or flasks. Autoclave for 10 min at 10 psi pressure– 115°C. Incubate for 1 week at 30°C to check for sterility before use.

Use: For the cultivation of Gemella morbillorum.

YGLM with Chalk (Yeast Glucose Litmus Milk with Chalk) Composition per liter: CaCO3 ......................................................................................... 20.0g Glucose ....................................................................................... 10.0g Yeast extract.................................................................................. 3.0g Litmus ......................................................................................... 0.75g Skim milk...............................................................................100.0mL

Preparation of Medium: Add components, except skim milk, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Add 10.0mL of skim milk. Distribute into tubes. Autoclave for 10 min at 10 psi pressure–115°C. Incubate for 1 week at 30°C to check for sterility before use.

Use: For the cultivation of Gemella morbillorum.

YGLPB Medium Composition per liter: Peptone........................................................................................ 10.0g Lab-Lemco.................................................................................... 8.0g Glucose ......................................................................................... 5.0g Lactose .......................................................................................... 5.0g Yeast extract.................................................................................. 3.0g KH2PO4 ........................................................................................ 2.5g K2HPO4 ........................................................................................ 2.5g MgSO4·7H2O................................................................................ 0.2g MnSO4·4H2O.............................................................................. 0.05g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 20 min at 15 psi pressure–121°C.

Use: For the cultivation of Lactobacillus delbrueckii.

YGLPB Medium Composition per liter: Peptone........................................................................................ 10.0g Beef extract ................................................................................... 8.0g Glucose ......................................................................................... 5.0g Lactose .......................................................................................... 5.0g Yeast extract.................................................................................. 3.0g K2HPO4 ........................................................................................ 2.5g KH2PO4 ........................................................................................ 2.5g © 2010 by Taylor and Francis Group, LLC

YGLPB Medium Composition per liter: Peptone ......................................................................................... 1.0g Lab Lemco (meat extract)............................................................. 0.8g Glucose ......................................................................................... 0.5g Lactose.......................................................................................... 0.5g Yeast extract.................................................................................. 0.3g K2HPO4....................................................................................... 0.25g KH2PO4....................................................................................... 0.25g MgSO4·7H2O .............................................................................. 0.02g MnSO4·4H2O ............................................................................. 5.0mg pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Carnobacterium gallinarum, Carnobacterium mobile, Enterococcus dispar, Lactobacillus fructivorans, Leuconostoc carnosum, Leuconostoc gelidum, and Vagococcus salmoninarum.

YGLPB Medium without Lactose Composition per liter: Peptone ....................................................................................... 10.0g Lab Lemco .................................................................................... 8.0g Yeast extract.................................................................................. 3.0g Glucose ......................................................................................... 5.0g KH2PO4 ........................................................................................ 2.5g K2HPO4 ........................................................................................ 2.5g MgSO4·7H2O................................................................................ 0.2g MnSO4·4H2O.............................................................................. 0.05g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 20 min at 15 psi pressure–121°C.

Use: For the cultivation of Vagococcus fluvialis and Vagococcus salmoninarum.

YI-S Medium Composition per liter: YI broth..................................................................................880.0mL Bovine serum, heat inactivated..............................................100.0mL Vitamin mixture 18 ................................................................. 20.0mL

Source: Vitamin mixture 18 is available from Bio-fluids, Inc., Rockville, MD.

YI Broth: Composition per liter: YI base stock......................................................................... 780.0mL 10X Glucose buffer stock ......................................................100.0mL

1946

YM-1L Broth

YI Base Stock: Composition per 780.0mL: Yeast extract................................................................................ 30.0g L-Cysteine·HCl.............................................................................. 1.0g NaCl .............................................................................................. 1.0g Ascorbic acid ................................................................................ 0.2g Ferric ammonium citrate........................................................ 228.0mg

10X Glucose Buffer Stock: Composition per 100.0.0mL: Glucose ....................................................................................... 10.0g K2HPO4 ......................................................................................... 1.0g KH2PO4 ......................................................................................... 0.6g

Preparation of 10X Glucose Buffer Stock: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of YI Base Stock: Add components to 600.0mL of distilled/deionized water. Mix thoroughly. Bring volume to 780.0mL with distilled/deionized water. Adjust pH to 6.8 with 1N NaOH. Distribute in 78.0mL aliquots to 100.0mL screw-capped bottles. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Preparation of YI Broth: Aseptically add 10.0mL of 10X glucose buffer stock to 78.0mL of cooled YI base stock. Adjust osmolarity with NaCl to 380.0milliosmols/kg.

Preparation of Medium: Aseptically add 2.0mL of vitamin mixture 18 and 10.0mL of heat-inactivated bovine serum to 88.0mL of YI broth. Distribute in 13.0mL aliquots to 16 x 125mm screw-capped test tubes. Store at 4°C in the dark with the caps screwed on tightly. Use within 96 hr.

Use: For the cultivation of Entamoeba species. YM Agar See: Yeast Malt Extract Agar YM Broth See: Yeast Malt Extract Broth YM Broth with 0.5%CaCO3 See:Yeast Malt Extract Broth with 0.5% CaCO3 YM Broth with 2.0%CaCO3 See:Yeast Malt Extract Broth with 2.0% CaCO3 YM Broth with Glucose See: Yeast Malt Extract Broth with Glucose

YM-1L Broth Composition per liter: Sodium lactate ............................................................................ 30.0g Mycological peptone .................................................................. 10.0g Succinic acid............................................................................... 10.0g NaOH............................................................................................ 6.0g Yeast extract.................................................................................. 5.0g Adenine....................................................................................... 0.01g Uracil .......................................................................................... 0.01g Yeast nitrogen base solution ..................................................100.0mL

Yeast Nitrogen Base Solution: Composition per 100.0mL: (NH4)2SO4 .................................................................................... 5.0g KH2PO4......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g NaCl.............................................................................................. 0.1g CaCl2·2H2O .................................................................................. 0.1g DL-Methionine............................................................................. 0.02g DL-Tryptophan............................................................................. 0.02g L-Histidine·HCl ........................................................................... 0.01g Inositol ....................................................................................... 2.0mg KI ............................................................................................... 0.1mg H3BO3 ........................................................................................ 0.5mg ZnSO4·7H2O .............................................................................. 0.4mg MnSO4·4H2O ............................................................................. 0.4mg Thiamine·HCl ............................................................................ 0.4mg Pyroxidine·HCl .......................................................................... 0.4mg Niacin......................................................................................... 0.4mg Calcium pantothenate ................................................................ 0.4mg p-Aminobenzoic acid................................................................. 0.2mg Riboflavin .................................................................................. 0.2mg FeCl3 .......................................................................................... 0.2mg Na2MoO4·4H2O ......................................................................... 0.2mg CuSO4·5H2O ............................................................................ 0.04mg Folic acid .................................................................................... 2.0μg Biotin .......................................................................................... 2.0μg

Preparation of Yeast Nitrogen Base Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except yeast nitrogen base solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure– 121°C. Aseptically add 1.0L of sterile yeast nitrogen base solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Saccharomyces cerevisiae.

YM Broth with 1.0% Methanol See: Yeast Malt Extract Broth with 1.0% Methanol

YM Medium See: Universal Agar for Yeasts

YM Broth with 18% NaCl See: Yeast Malt Extract Broth with 18% NaCl

YM Medium (DSMZ Medium 1070)

YM Broth with 40% Sucrose See: Yeast Malt Extract Broth with 40% Sucrose YM Broth with 70% Sucrose See: Yeast Malt Extract Broth with 70% Sucrose YM Catalase Agar See: Yeast Malt Extract Catalase Agar © 2010 by Taylor and Francis Group, LLC

Composition per liter: Mannitol ..................................................................................... 10.0g Yeast extract ................................................................................. 0.5g K2HPO4......................................................................................... 0.5g NaCl ............................................................................................. 0.2g CaCl2·2H2O .................................................................................. 0.2g MgSO4·7H2O ................................................................................ 0.1g pH 7.0 ± 0.2 at 25°C

YMF Broth Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Labrys miyagiensis.

YM5 with 10% Sorbitol Composition per liter: Sorbitol...................................................................................... 100.0g Glucose ....................................................................................... 10.0g Succinic acid ............................................................................... 10.0g NaOH ............................................................................................ 6.0g Peptone.......................................................................................... 2.0g Yeast extract.................................................................................. 1.0g Adenine ....................................................................................... 0.01g Uracil .......................................................................................... 0.01g Yeast nitrogen base solution ..................................................100.0mL pH 5.8 ± 0.2 at 25°C

Yeast Nitrogen Base Solution: Composition per 100.0mL: (NH4)2SO4 ..................................................................................... 5.0g KH2PO4 ......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g NaCl .............................................................................................. 0.1g CaCl2·2H2O................................................................................... 0.1g DL-Methionine............................................................................. 0.02g DL-Tryptophan............................................................................. 0.02g L-Histidine·HCl ........................................................................... 0.01g Inositol ....................................................................................... 2.0mg KI ............................................................................................... 0.1mg H3BO3 ........................................................................................ 0.5mg ZnSO4·7H2O .............................................................................. 0.4mg MnSO4·4H2O ............................................................................. 0.4mg Thiamine·HCl ............................................................................ 0.4mg Pyroxidine·HCl .......................................................................... 0.4mg Niacin......................................................................................... 0.4mg Calcium pantothenate ................................................................ 0.4mg p-Aminobenzoic acid................................................................. 0.2mg Riboflavin .................................................................................. 0.2mg FeCl3 .......................................................................................... 0.2mg Na2MoO4·4H2O ......................................................................... 0.2mg CuSO4·5H2O ............................................................................ 0.04mg Folic acid.....................................................................................2.0μg Biotin ..........................................................................................2.0μg

1947

YMA Agar Composition per liter: Agar ............................................................................................ 15.0g Mannitol...................................................................................... 10.0g CaCO3 ........................................................................................... 4.0g KH2PO4......................................................................................... 0.5g Yeast extract.................................................................................. 0.4g MgSO4·7H2O ................................................................................ 0.2g NaCl.............................................................................................. 0.1g pH 6.8 ± 0.2 at 25°C

Use: For the cultivation of Rhizobium fredii, Rhizobium galegae, Rhizobium huakuii, Rhizobium leguminosarum, Rhizobium loti, Rhizobium meliloti, Rhizobium phaseoli, and Rhizobium trifolii.

YMA Medium (DSMZ Medium 1031) Composition per liter: Agar ............................................................................................ 20.0g Mannitol ..................................................................................... 10.0g Yeast extract ................................................................................. 0.3g MgSO4·7H2O ................................................................................ 0.2g K2HPO4......................................................................................... 0.2g NaCl ........................................................................................... 0.05g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Phyllobacterium trifolii.

YMF Agar Composition per liter: Agar ............................................................................................ 20.0g Peptone ......................................................................................... 5.0g Sugar, brown ................................................................................. 3.0g Malt extract................................................................................... 3.0g Yeast extract.................................................................................. 3.0g pH 6.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

Source: Yeast nitrogen base is available as a premixed powder from BD Diagnostic Systems.

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.2. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Preparation of Yeast Nitrogen Base Solution: Add components

Use: For the cultivation of various fungi.

to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

YMF Broth

Preparation of Medium: Add components, except yeast nitrogen

Composition per liter:

base solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to room temperature. Aseptically add 100.0mL of sterile yeast nitrogen base solution to the cooled sterile basal medium. Mix thoroughly. Adjust final pH to 5.8. Distribute into sterile flasks or tubes.

Peptone ......................................................................................... 5.0g Sugar, brown ................................................................................. 3.0g Malt extract................................................................................... 3.0g Yeast extract.................................................................................. 3.0g pH 6.2 ± 0.2 at 25°C

Use: For the cultivation of a sorbitol-utilizing fungus.

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.2.

Preparation of Medium: Add components to distilled/deionized

1948

YNA Medium

Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation of various fungi.

YNA Medium (Yeast Extract Nutrient Agar Medium) Composition per liter: Agar ............................................................................................ 15.0g NaCl .............................................................................................. 5.0g Peptone.......................................................................................... 5.0g Meat extract .................................................................................. 4.0g Yeast extract.................................................................................. 2.5g pH 7.0 ± 0.2 at 25°C

Use: For the isolation and cultivation of Kurthia species according to the agar streak method.

YNG Medium (Yeast Extract Nutrient Gelatin Medium) Composition per liter: Gelatin....................................................................................... 100.0g NaCl .............................................................................................. 5.0g Peptone.......................................................................................... 5.0g Meat extract .................................................................................. 4.0g Yeast extract.................................................................................. 2.5g pH 7.0 ± 0.2 at 25°C

Use: For the isolation and cultivation of Kurthia species using the gelatin streak method.

Yolk Milk Medium (YOM) Composition per liter: Egg yolk .................................................................................500.0mL Milk........................................................................................500.0mL

Egg Yolk: Composition per 500.0mL: Chicken egg yolks................................................................... variable

Preparation of Egg Yolk: Soak eggs with 1:100 dilution of saturated mercuric chloride solution for 1 min. Crack eggs and separate yolks from whites. Add sufficient egg yolk to bring volume to 500.0mL. Mix thoroughly.

Preparation of Milk: Autoclave 500.0mL of milk for 20 min at 15 psi pressure–115°C.

Preparation of Medium: Combine 500.0mL of sterile egg yolk and 500.0mL of sterile milk. Mix thoroughly. Distribute into sterile tubes or flasks. Heat to 95°C for 20–25 min.

Yopp’s Medium Composition per liter: NaCl........................................................................................ 116.88g MgCl2·6H2O ............................................................................. 10.68g MgSO4·7H2O .............................................................................. 10.0g KCl................................................................................................ 2.0g CaNO3·4H2O ................................................................................ 1.0g Glycyl-glycine buffer.................................................................... 0.5g K2HPO4·3H2O .......................................................................... 0.065g Ferric EDTA .............................................................................. 5.0mg Trace metals solution .................................................................1.0mL pH 7.8 ± 0.2 at 25°C

Trace Metals Solution: Composition per liter: MnCl2·4H2O ................................................................................. 2.0g H3BO3 ........................................................................................... 0.5g ZnNO3·6H2O ................................................................................ 0.5g Co(NO3)2·6H2O ........................................................................ 0.025g CuCl2·2H2O .............................................................................. 0.025g Na2MoO4·2H2O ........................................................................ 0.025g VOSO4·6H2O............................................................................ 0.025g HCl.............................................................................................3.0mL

Preparation of Trace Metals Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Use: For the isolation and cultivation of halophilic cyanobacteria.

YP87 Medium Composition per liter: Na2SO4 .......................................................................................... 4.0g NaHCO3 ........................................................................................ 1.3g KCl................................................................................................ 0.5g Yeast extract.................................................................................. 0.5g MgCl2·6H2O ................................................................................. 0.4g NH4Cl ......................................................................................... 0.25g L-Ascorbic acid ............................................................................. 0.2g Na2HPO4 ...................................................................................... 0.2g Sodium thioglycolate .................................................................... 0.2g CaCl2·2H2O ................................................................................ 0.15g Resazurin ................................................................................... 1.0mg Modified Wolfe’s mineral solution ..........................................10.0mL Wolfe’s vitamin solution..........................................................10.0mL Sodium lactate, 60% syrup ........................................................3.0mL pH 7.5 ± 0.2 at 25°C

Modified Wolfe’s Mineral Solution: Composition per liter: MgSO4·7H2O ................................................................................ 3.0g Nitrilotriacetic acid ....................................................................... 1.5g NaCl.............................................................................................. 1.0g MnSO4·H2O .................................................................................. 0.5g CaCl2 ............................................................................................. 0.1g CoCl2·6H2O .................................................................................. 0.1g FeSO4·7H2O.................................................................................. 0.1g ZnSO4·7H2O ................................................................................. 0.1g AlK(SO4)2·12H2O....................................................................... 0.01g CuSO4·5H2O ............................................................................... 0.01g

YPDP Medium with 5´-TMP

H3BO3 ......................................................................................... 0.01g Na2MoO4·2H2O .......................................................................... 0.01g Na2SeO3 ...................................................................................... 0.01g NaWO4·2H2O.............................................................................. 0.01g NiC12·6H2O ................................................................................ 0.01g

1949

Use: For the cultivation of Pasteurella multocida.

YPD Medium (DSMZ Medium 393) Composition per liter: Peptone ....................................................................................... 20.0g Glucose ....................................................................................... 20.0g Yeast extract................................................................................ 10.0g pH 6.5 ± 0.2 at 25°C

Use: For the isolation and cultivation of Yarrowia lipolytica (Candida lipolytica), Kluyveromyces spp., Saccharomyces spp., Pichia spp., and Candida spp.

Preparation of Wolfe’s Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Prepare and dispense medium under 100% N2. Add components, except L-ascorbic acid, NaHCO3, and sodium thioglycolate, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5. Gently heat and bring to boiling. Cool while sparging with 100% N2. Add L-ascorbic acid, NaHCO3, and sodium thioglycolate. Mix thoroughly. Sparge with 100% N2. Distribute into tubes or bottles. Autoclave for 15 min at 15 psi pressure–121°C. Use: For the cultivation of Thermodesulfovibrio yellowstonii.

YPAD Medium for MAK Mutants of Saccharomyces Composition per liter: Peptone........................................................................................ 20.0g Glucose ....................................................................................... 20.0g Agar ............................................................................................ 20.0g Yeast extract................................................................................ 10.0g Adenine sulfate ............................................................................. 0.4g

Use: For the cultivation and maintenance of a variety of yeasts, including Candida albicans, Candida boidinii, Candida pintolopesii, Saccharomyces cerevisiae, and Schizosaccharomyces pombe.

YPDA (Yeast Peptone Dextrose Agar) Composition per liter: Agar ............................................................................................ 20.0g Glucose ....................................................................................... 20.0g Peptone ....................................................................................... 20.0g Yeast extract................................................................................ 10.0g Adenine sulfate ............................................................................. 0.4g

Use: For the cultivation of Taphrina populina.

YPDP Medium with 5´-TMP Composition per liter: Glucose ....................................................................................... 20.0g Peptone ....................................................................................... 20.0g Agar ............................................................................................ 15.0g Yeast extract................................................................................ 10.0g KH2PO4......................................................................................... 1.5g Thymidine-5´-monophosphate solution...................................10.0mL

Thymidine-5´-Monophosphate Solution: Composition per 10.0mL:

YPC Medium

Thymidine-5´-monophosphate .............................................. 100.0mg

Composition per liter:

Preparation of Thymidine-5´-Monophosphate Solution: Add

Agar ............................................................................................ 15.0g Proteose peptone ......................................................................... 15.0g Yeast extract.................................................................................. 5.0g KH2PO4 ......................................................................................... 4.0g Sucrose.......................................................................................... 2.5g Glucose ......................................................................................... 2.0g L-Cystine ....................................................................................... 0.5g Na2SO3 .......................................................................................... 0.2g pH 7.2 ± 0.2 at 25°C

thymidine-5´-monophosphate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except thymidine-5´monophosphate solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 10.0mL of sterile thymidine-5´-monophosphate solution. Mix thoroughly. Pour into sterile Petri dishes or aseptically distribute into sterile tubes.

1950

YPG Agar with 2% Sodium Chloride

Use: For the cultivation and maintenance of Saccharomyces cerevi-

Use: For the cultivation of a variety of yeasts and other fungi.

siae.

YPG Agar with 2% Sodium Chloride Composition per liter: Agar ............................................................................................ 20.0g Glucose ....................................................................................... 20.0g NaCl ............................................................................................ 20.0g Peptone........................................................................................ 10.0g Yeast extract................................................................................ 10.0g pH 5.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.2. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

YPG Medium (DSMZ Medium 1017) Composition per liter: Glucose ...................................................................................... 70.0gl Yeast extract ............................................................................... 10.0g Peptone........................................................................................ 10.0g pH 6.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.0 with dilute HCl. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Saccharibacter floricola.

YPG Medium (DSMZ Medium 1172)

YPM Agar Composition per liter: Mannitol...................................................................................... 25.0g Agar ............................................................................................ 12.0g Yeast extract.................................................................................. 5.0g Peptone ......................................................................................... 3.0g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Do not adjust pH. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Acetobacter aceti, Acetobacter pasteurianus, Acetobacter xylinum, Frateuria aurantia, and Pseudomonas aeruginosa.

YPNC Medium Composition per liter: Agar ............................................................................................ 18.0g NaCl............................................................................................ 2.92g KH2PO4..................................................................................... 0.596g Yeast extract.................................................................................. 0.5g Sodium hydrogen glutamate (pH 6.0) ........................................ 0.37g K2HPO4..................................................................................... 0.107g NH4Cl ....................................................................................... 0.107g MgSO4·7H2O ............................................................................ 0.049g Glucose solution ......................................................................10.0mL Trace metals solution .................................................................1.0mL pH 6.0 ± 0.2 at 25°C

Glucose Solution: Composition per 10.0mL: Glucose ......................................................................................... 4.0g

Composition per liter:

Preparation of Glucose Solution: Add glucose to distilled/deion-

Yeast extract ................................................................................. 1.0g Peptone ......................................................................................... 1.0g Glucose ........................................................................................ 1.0g pH 5.7 ± 0.2 at 25°C

ized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.6–6.0 with dilute HCl. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Asticcacaulis benevestitus.

YPGA (DSMZ Medium 1015) Composition per liter: Agar ........................................................................................... 15.0g Yeast extract ................................................................................. 7.0g Peptone ......................................................................................... 7.0g Glucose ........................................................................................ 7.0g pH 7.3 ± 0.2 at 25°C

Trace Metals Solution: Composition per liter: EDTA .......................................................................................... 50.0g ZnSO4·7H2O ............................................................................... 22.0g CaCl2 ........................................................................................... 5.54g MnCl2·4H2O ............................................................................... 5.06g FeSO4·7H2O................................................................................ 4.99g (NH4)6Mo7O14·H2O .................................................................... 1.10g CoSO4·5H2O ............................................................................... 1.57g CoCl2·6H2O ................................................................................ 1.61g

Preparation of Trace Metals Solution: Add components, one at a time, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except glucose solution and trace metals solution, to distilled/deionized water and bring volume to 989.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 6.0 with KOH. Autoclave for 15 min at 15 psi pressure– 121°C. Cool to 50°C. Aseptically add 10.0mL of sterile glucose solution and 1.0mL of sterile trace metals solution. Mix thoroughly. Pour into sterile Petri dishes or aseptically distribute into sterile tubes.

Use: For the cultivation and maintenance of a variety of Cryptococcus species.

YPSC Medium

YPS Medium (DSMZ Medium 990) Composition per liter: Sea salts, Sigma .......................................................................... 35.0g Sulfur, elemental ........................................................................... 5.0g PIPES (piperazine-N,N'-bis[2-ethane-sulfonic acid]) ................ 3.46g NH4Cl .......................................................................................... 0.5g KH2PO4 ...................................................................................... 0.35g CaCl2·2H2O................................................................................... 0.2g FeCl3·6H2O ................................................................................ 6.7mg Na2WO4 .................................................................................... 2.9mg Resazurin .................................................................................. 0.1mg Yeast extract solution ...............................................................10.0mL Peptone solution.......................................................................10.0mL Sulfide solution ..........................................................................5.0mL pH 6.8 ± 0.2 at 25°C

Sulfide Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.5g

Preparation of Sulfide Solution: Add Na2S·9H2O to distilled/de-

ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room temperature. Adjust pH to 7.0.

Yeast Extract Solution: Composition per 10.0mL: Yeast extract ................................................................................. 1.0g

Peptone Solution: Composition per 10.0mL: Peptone ......................................................................................... 4.0g

Preparation of Peptone Solution: Add peptone to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature. Sparge with 100% N2.

Preparation of Medium: Add components, except yeast extract, peptone, and sulfide solutions, to distilled/deionized water and bring volume to 975.0mL. Mix thoroughly. Gently heat and bring to boiling. Cool to room temperature while sparging with 100% N2. Adjust pH to 6.8. Prepare the medium without the yeast extract, peptone, and sodium sulfide. Boil the medium and cool under nitrogen. Adjust the pH to 6.8. Dispense into Hungate tubes or serum bottles under a nitrogen atmosphere. Sterilize the medium at 100°C for 3 hr on 3 consecutive days. Aseptically add the peptone and yeast extract solutions. Adding the sterile, neutralized sulfide solution to an end concentration of 0.025%. Final pH should be 6.8.

Use: For the cultivation of Thermococcus marinus.

YPS Medium (DSMZ Medium 1168) Composition per liter: Sea salts, Sigma .......................................................................... 25.0g Yeast extract ................................................................................. 4.0g Peptone ......................................................................................... 2.0g pH 7.5 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

1951

Use: For the cultivation of Owenweeksia hongkongensis.

YPSC Agar (Yeast Extract Peptone Sulfate Cysteine Agar) Composition per liter: Agar ............................................................................................ 15.0g Yeast extract.................................................................................. 1.0g Peptone ......................................................................................... 1.0g Sodium acetate·3H2O.................................................................... 0.5g MgSO4·7H2O .............................................................................. 0.25g CaCl2·2H2O ................................................................................ 0.25g L-Cysteine·HCl·H2O ................................................................... 0.05g pH 7.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Adjust pH to 7.5 with sterile 10M NaOH. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Bdellovibrio species.

YPSC Agar, Cation-Supplemented Composition per liter: Sodium acetate·3H2O.................................................................. 50.0g Agar ............................................................................................ 15.0g Peptone ....................................................................................... 10.0g Yeast extract................................................................................ 10.0g MgSO4·7H2O .............................................................................. 0.74g CaCl2·2H2O ................................................................................ 0.29g L-Cysteine·HCl·H2O ................................................................... 0.05g Bacitracin solution ...................................................................10.0mL

Bacitracin Solution: Composition per 10.0mL: Bacitracin................................................................................. 6,000U

Preparation of Bacitracin Solution: Add bacitracin to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, except bacitracin solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile bacitracin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and enumeration of Bdellovibrio species.

YPSC Medium (Yeast Extract Peptone Sulfate Cysteine Medium) Composition per liter: Yeast extract.................................................................................. 1.0g Peptone ......................................................................................... 1.0g Sodium acetate·3H2O.................................................................... 0.5g MgSO4·7H2O .............................................................................. 0.25g CaCl2·2H2O ................................................................................ 0.25g L-Cysteine·HCl·H2O ................................................................... 0.05g pH 7.5 ± 0.2 at 25°C

1952

YPSC Soft Agar

Use: For the cultivation and enumeration of Bdellovibrio species.

YPSC Soft Agar (Yeast Extract Peptone Sulfate Cysteine Soft Agar)

Peptone ....................................................................................... 10.0g Yeast extract.................................................................................. 5.0g pH 7.0 ± 0.2 at 25°C

Use: For the cultivation of Xanthomonas albilineans.

Composition per liter:

YT HiVeg Broth

Agar .............................................................................................. 6.0g Yeast extract.................................................................................. 1.0g Peptone.......................................................................................... 1.0g Sodium acetate·3H2O.................................................................... 0.5g MgSO4·7H2O .............................................................................. 0.25g CaCl2·2H2O................................................................................. 0.25g L-Cysteine·HCl·H2O.................................................................... 0.05g pH 7.5 ± 0.2 at 25°C

Plant hydrolysate ........................................................................ 16.0g Yeast extract................................................................................ 10.0g NaCl.............................................................................................. 5.0g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Adjust pH to 7.5 with sterile 10M NaOH. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Bdellovibrio species.

YpSs Agar Composition per liter: Agar ............................................................................................ 15.0g Soluble starch.............................................................................. 15.0g Yeast extract.................................................................................. 4.0g K2HPO4 ......................................................................................... 1.0g MgSO4·7H2O ................................................................................ 0.5g

Preparation of Medium: Add components to distilled/deionized

Composition per liter:

Source: This medium is available as a premixed powder from HiMedia. water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Escherichia coli.

YT Medium (Yeast Extract Tryptone Medium) Composition per liter: Pancreatic digest of casein............................................................ 8.0g Yeast extract.................................................................................. 5.0g NaCl.............................................................................................. 5.0g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Escherichia coli.

Use: For the cultivation of bacteria that can utilize starch as a carbon

Composition per liter:

source.

YPSS, Emerson Agar Composition per liter: Agar ............................................................................................ 15.0g Soluble starch.............................................................................. 15.0g Yeast extract.................................................................................. 4.0g K2HPO4 ........................................................................................ 1.0g MgSO4·7H2O................................................................................ 0.5g

Use: For the cultivation and maintenance of Allomyces javanicus, Melanospora tiffanii, and Sporothrix schenckii.

YSP Agar Composition per liter: Sucrose........................................................................................ 20.0g Agar ............................................................................................ 12.0g © 2010 by Taylor and Francis Group, LLC

YTG Medium Tryptone...................................................................................... 10.0g Na2CO3 ........................................................................................ 5.3g Yeast extract.................................................................................. 5.0g Na2HPO4·2H2O......................................................................... 0.356g L-Cysteine·HCl ............................................................................. 0.2g Na2S·9H2O.................................................................................... 0.2g KCl............................................................................................ 0.075g Resazurin ................................................................................... 1.0mg Glucose solution ......................................................................20.0mL pH 10.1 ± 0.2 at 25°C

Glucose Solution: Composition per 20.0mL: D-Glucose...................................................................................... 3.0g

Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Prepare and dispense medium under 100% N2. Add components, except glucose solution, to distilled/deionized water and bring volume to 980.0L. Mix thoroughly. Sparge with 100% N2 for 30 min. Anaerobically distribute 9.8mL volumes into tubes. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and

Zavarzinella formosa Medium

1953

anaerobically add 0.2mL of sterile glucose solution to each tube. Adjust pH to 10.1 with sterile anaerobic 3N NaOH solution.

Acetamide Solution: Composition per 100.0mL:

Use: For the cultivation of Clostridium paradoxum and Clostridium thermoalcaliphilum.

Acetamide ................................................................................... 10.0g

YTN Medium (Yeast Extract Tryptone NaCl Medium) Composition per liter: NaCl ............................................................................................ 30.0g Agar ............................................................................................ 15.0g Yeast extract................................................................................ 10.0g Pancreatic digest of casein .......................................................... 10.0g Glucose ......................................................................................... 1.0g Trace elements solution .............................................................1.0mL pH 7.2 ± 0.2 at 25°C

Trace Elements Solution: Composition per liter: H3BO3 ......................................................................................... 2.85g MnCl2·4H2O.................................................................................. 1.8g Sodium tartrate............................................................................ 1.77g FeSO4 .......................................................................................... 1.36g CoCl2·6H2O ................................................................................ 0.04g CuCl2·2H2O............................................................................... 0.027g Na2MoO4·2H2O ........................................................................ 0.025g ZnCl2 ......................................................................................... 0.021g pH 7.2 ± 0.2 at 25°C

Preparation of Acetamide Solution: Add acetamide to distilled/ deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except acetamide solution, to distilled/deionized water and bring volume to 960.0mL. Mix thoroughly. Autoclave for 20 min at 15 psi pressure–121°C. Cool to 70°C. Aseptically add 40.0mL sterile acetamide solution. Mix thoroughly. Pour into sterile Petri dishes. Dry plates at 37°C for 30 min.

Use: For the isolation of Pseudomonas aeruginosa from milk.

Z Broth Composition per liter: Acetamide ..................................................................................... 5.0g K2HPO4......................................................................................... 5.0g KH2PO4......................................................................................... 3.0g KNO3 ............................................................................................ 1.0g K2S4O6 .......................................................................................... 1.0g MgSO4·7H2O .............................................................................. 0.05g pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Cool. Aseptically distribute 10.0mL volumes into test tubes containing inverted Durham tubes. Heat for 15 min at 0 psi pressure–100°C. Use: For the cultivation of Pseudomonas aeruginosa from milk.

Preparation of Trace Elements Solution: Add components to

Z Medium

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

YTSS Medium, Half Strength (DSMZ Medium 974) Composition per liter: Sea salts....................................................................................... 20.0g Yeast extract.................................................................................. 2.0g Tryptone ...................................................................................... 1.25g pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Roseovarius nubinhibens and Silicibacter pomeroyi.

Z Agar Composition per liter: Agar ....................................................................................16.0 K2HPO4 ......................................................................................... 5.0g K2SO4 ............................................................................................ 2.0g KH2PO4 ......................................................................................... 1.0g MgSO4·7H2O .............................................................................. 0.05g Acetamide solution ..................................................................40.0mL pH 7.2 ± 0.2 at 25°C © 2010 by Taylor and Francis Group, LLC

Composition per liter: Casein hydrolysate...................................................................... 10.0g NaCl............................................................................................ 10.0g Yeast extract.................................................................................. 5.0g Glucose ......................................................................................... 1.0g CaCl2·2H2O .............................................................................. 0.367g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Alcaligenes eutrophus.

Zavarzinella formosa Medium (DSMZ Medium 1196) Composition per liter: N-acetylglucosamine ................................................................... 1.0g Glucose ........................................................................................ 0.5g KH2PO4 ........................................................................................ 0.1g Peptone ........................................................................................ 0.1g Yeast extract ................................................................................. 0.1g MgSO4·7H2O ................................................................................ 0.1g Casamino acids ............................................................................ 0.1g CaCl2·2H2O ................................................................................ 0.05g NaCl ........................................................................................... 0.01g “Metals 44”................................................................................1.0mL pH 5.9 ± 0.2 at 25°C

1954

ZF2 Medium

Sodium EDTA............................................................................. 0.25g MnSO4·H2O.............................................................................. 0.154g CuSO4·5H2O ............................................................................ 39.2mg Co(NO3)2·6H2O ....................................................................... 24.8mg Na2B4O7·10H2O....................................................................... 17.7mg

Preparation of Dithionite Solution: Add Na2-dithionite to distilled/

Pyridoxine hydrochloride ...................................................... 300.0mg Thiamine-HCl·2H2O.............................................................. 200.0mg Nicotinic acid......................................................................... 200.0mg Vitamin B12 ............................................................................ 100.0mg Calcium pantothenate ............................................................ 100.0mg p-Aminobenzoic acid............................................................... 80.0mg D(+)-Biotin............................................................................... 20.0mg

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.8–6.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure– 121°C.

Use: For the cultivation of Zavarzinella formosa.

ZF2 Medium (DSMZ Medium 943) Composition per liter:

deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Seven Vitamin Solution: Composition per liter:

Preparation of Seven Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Sparge with 100% N2. Mix thoroughly. Filter sterilize. Phosphate Buffer: Composition per liter: Na2HPO4·12H2O......................................................................... 43.0g KH2PO4....................................................................................... 5.44g

NaHCO3 ........................................................................................ 3.8g Yeast extract.................................................................................. 3.0g (NH4)HCO3 ................................................................................. 0.45g MgSO4·6H2O .............................................................................. 0.13g CaCl2·2H2O................................................................................. 0.12g Resazurin ................................................................................... 0.5mg Phosphate buffer ......................................................................10.0mL Glycine solution .........................................................................5.0mL Arginine solution .......................................................................5.0mL Na2S·9H2O solution ...................................................................5.0mL Dithionite solution .....................................................................1.0mL Seven vitamin solution...............................................................1.0mL Trace elements solution SL-10 ..................................................1.0mL Selenite-tungstate solution .........................................................1.0mL pH 7.3 ± 0.2 at 25°C

Preparation of Phosphate Buffer: Add components to distilled/ deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.

Arginine Solution: Composition per 10.0mL:

Arginine-HCl ................................................................................ 3.5g

Preparation of Arginine Solution: Add arginine-HCl to distilled/ deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Trace Elements Solution SL-10: Composition per liter: FeCl2·4H2O ................................................................................... 1.5g CoCl2·6H2O ........................................................................... 190.0mg MnCl2·4H2O .......................................................................... 100.0mg ZnCl2 ........................................................................................ 70.0mg Na2MoO4·2H2O ....................................................................... 36.0mg NiCl2·6H2O .............................................................................. 24.0mg H3BO3 ........................................................................................ 6.0mg CuCl2·2H2O ............................................................................... 2.0mg HCl (25% solution)..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

Selenite-Tungstate Solution Composition per liter:

Glycine........................................................................................ 15.0g

NaOH............................................................................................ 0.5g Na2WO4·2H2O ........................................................................... 4.0mg Na2SeO3·5H2O........................................................................... 3.0mg

Preparation of Glycine Solution: Add glycine to distilled/deion-

Preparation of Selenite-Tungstate Solution: Add components

ized water and bring volume to 100.0mL. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Preparation of Medium: Prepare and dispense medium under 80%

Glycine Solution: Composition per 100.0mL:

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 100% N2. Filter sterilize.

Dithionite Solution: Composition per 10.0mL:

N2 + 20% CO2 gas mixture. Add components, except phosphate buffer, glycine solution, arginine solution, dithionite solution, seven vitamin solution, and Na2S·9H2O solution, to distilled/deionized water and bring volume to 973.0mL. Mix thoroughly. Equilibrate with 80% N2 + 20% CO2 to reach pH 7.3. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically and anaerobically add 10.0mL phosphate buffer, 5.0mL glycine solution, 5.0mL arginine solution, 1.0mL dithionite solution, 1.0mL seven vitamin solution, and 5.0mL Na2S·9H2O solution. Mix thoroughly. Aseptically and anaerobically distribute into sterile tubes or flasks.

Na2-dithionite.............................................................................. 0.25g

Use: For the cultivation of Sedimentibacter saalensis.

Na2S·9H2O Solution: Composition per 10.0mL: Na2S·9H2O .................................................................................... 0.3g

Zymomonas Sucrose Medium

Zoogloea Medium Composition per liter:

1955

Use: For the cultivation and maintenance of Zymomonas mobilis and

Preparation of Medium: Add components to distilled/deionized

Composition per liter:

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into screw-cap test tubes. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Zoogloea ramigera and other Zoogloea species.

Zymobacterium Agar Composition per liter: Pancreatic digest of casein .......................................................... 20.0g Agar ............................................................................................ 15.0g Orotic acid..................................................................................... 2.0g Na3PO4·12H2O.............................................................................. 1.5g Sodium thioglycolate .................................................................... 1.0g NaOH ............................................................................................ 0.5g Riboflavin ................................................................................. 0.015g pH 7.9 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized water in the following order: tryptone, orotic acid, sodium hydroxide, sodium phosphate, sodium thioglycolate, riboflavin, agar and bring volume to 1.0L . Mix thoroughly. Some orotic acid will remain undissolved. Adjust pH to 7.9 using NaH2PO4. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Mix thoroughly to dissolve orotic acid. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Clostridium (Zymobacterium) oroticum.

Zymobacterium Broth Composition per liter: Pancreatic digest of casein .......................................................... 20.0g Orotic acid..................................................................................... 2.0g Na3PO4·12H2O.............................................................................. 1.5g Sodium thioglycolate .................................................................... 1.0g NaOH ............................................................................................ 0.5g Riboflavin ................................................................................. 0.015g pH 7.9 ± 0.2 at 25°C

other Zymomonas species.

Zymomonas Medium Glucose ....................................................................................... 20.0g Agar ............................................................................................ 15.0g Peptone ....................................................................................... 10.0g Yeast extract................................................................................ 10.0g pH 6.8 ± 0.2 at 25°C

Use: For the cultivation of Zymomonas anaerobia.

Zymomonas Medium Composition per liter: Glucose ....................................................................................... 10.0g Yeast extract................................................................................ 10.0g pH 4.8 ± 0.2 at 25°C

Preparation of Medium: Add components to tap water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 4.8. Distribute into tubes or flasks. Autoclave for 20 min at 15 psi pressure–121°C. Boil the medium immediately before use. Use: For the cultivation of Zymomonas mobilis and other Zymomonas species.

Zymomonas Medium (ATCC Medium 845) (ATCC Medium 948) Composition per liter: Glucose ....................................................................................... 20.0g Yeast extract.................................................................................. 5.0g

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Zymomonas mobilis and other Zymomonas species.

Preparation of Medium: Add components to distilled/deionized water in the following order: tryptone, orotic acid, sodium hydroxide, sodium phosphate, sodium thioglycolate, riboflavin and bring volume to 1.0L. Mix thoroughly. Some orotic acid will remain undissolved. Adjust pH to 7.9 using NaH2PO4. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Mix again to dissolve orotic acid.

Use: For the cultivation and maintenance of Clostridium (Zymobacterium) oroticum.

Zymomonas Agar Composition per liter: Glucose ....................................................................................... 20.0g Agar ............................................................................................ 15.0g Yeast extract.................................................................................. 5.0g © 2010 by Taylor and Francis Group, LLC

Zymomonas Sucrose Medium Composition per liter: Sucrose...................................................................................... 150.0g Yeast extract.................................................................................. 2.0g Peptone ......................................................................................... 2.0g KH2PO4......................................................................................... 2.0g (NH4)2SO4 .................................................................................... 2.0g MgSO4·7H2O ................................................................................ 2.0g pH 7.0 ± 0.2 at 25°C

Index

A A 1 Broth. 11 A 3 Agar, 12 A 3B Agar. 12 A 7 Agar, 13 A 7 Agar, Modified. 13 A 7B Agar, 14 A 8B Agar, 15 A Medium, 5X, 11 A-l I liVegBroth.il Al- Medium, 11 Al Minimal Medium. 12 A2E6 Medium. 59 AAI1C Medium tor YAK Clones, 15 A AM Medium. 16 AATCC Bacteriostasis Agar, 16, 670 AAICC Bacteriostasis Broth, 670 AATCC Bacteriostasis HiVeg Agar. 16 AATCC Bacteriostasis HiVeg Broth, 16 AATCC Mineral Salts Iron Agar, 16 Abeyta-Hunt Bark Agar, 16 ABY Agar, 17 AC Agar. 17 AC Broth, 17 ACHtVegAgar, 17 AC HiVeg Broth. 18 AC Medium, 17 Acanlhamoeba astmnyxis, 706, 1457 Acanlhamoeha castellanii, 706, 1457 Acanlhamoeba comandoni, 1457 Acanlhamoeba culbertsoni. 1457 Acanlhamoeba divionensis, 1457 Acanlhamoeba grijffini. 706 Acanlhamoeba halchelli, 1454 Acanlhamoeba healyi, 1457 Acanlhamoeba jacohsi. 1454 Acanlhamoeba leniiculata, 1457 Acanlhamoeba lugdunensis. 1457 Acanlhamoeba mauritaniensis, 1457 Acanlhamoeba Medium, 18 Acanthamoclxi palestinensis. 1457 Acanlhamoeba pearcei. 706, 1457 Acanlhamoeba polyphaga, 706, 1454, 1457 Acanlhamoeba pustulosa, 1457 Acanlhamoeba quina, 1457 Acanlhamoeba rhysodes, 706, 1457 Acanlhamoeba royreba. 1457 Acanlhamoeba species, 18, 1457 Acanlhamoeba slewnsoni, 706 Acanlhamoeba lerricola, 1457 Acanlhamoeba triangularis. 1457 Acanlhamoeba tubiashi. 706 Acanthoecopsis unguiculata, 1015 Acartxspora fuscafa, 951 Acarosjwra smaragdula, 951 ACB90-Medium, 18 ACC Medium, 19 ACE Medium. 19 Acetamide Agar, 20 Acetamide Brotli, 20

Acetamide Cetrimide Glycerol Mannitol Selective Medium, 20 Acetamide Medium. 20. 21 Acetamide Nutrient Biolh, 21 Acetate Agar. 21. 22 Acetate Differential Agar, 22 Acetate fermentation, 393 Acetate I liVeg Agar, 22 Acetate-utilizing methanogenic bacteria. 1099 Acetic Acid Agar. 22 Acetic acid bacteria. 23 Acetic Acid Bacterium Medium, 23 Acetic Acid Brotli. 23 Acelilomacidum Medium. 23 Aceiitomaculum rtiminus, 24, 25 Acelilomacidum ruminus Medium, 24 Acelivibrio celhdolylicus. 25, 200 Acelivihiio celhdolylicus Medium, 25 Acelivibrio eellulosolvens. 200 Acelivibrio Desulfobvihrio Medium, 25 Acelivibrio ethanolgignens. 26,926. 1369, 1459 Acelivibrio mullivorans, 1434 Acelobacter aceli, 27, 28, 742, 1005, 1932, 1933.1950 Acetobacler Agar, 26, 27 Acelobacter Agar (Glucose), 27 Acetobacler Broth, 27 Acelobacter diazolrophicus, 27, 28 Acetobacler diazotrophicus Agar. 27 Acetobacler europaeus, 27 Acetofxicier europaeus Medium. 27 Acetobaclerfaharum, 1008 Acelobacter hansenii, 27.28. 1005. 1932 Acelobacter I liVeg Agar with Plant Extract, 28 Acetobacler liquef'aciens, 27. 28, 1932 Acelobacter Medium, 28 Acetobacler methanolicus. 1629 Acelobacter pasleurianus, 28,318,586,742, 1005.1394. 1395.1932, 1933.1950 Acelobacter peroxydans, 28 Acetofiacter peroxydans Medium, 28 Acetobacler pomorum, 1486 Acelobacter species, 27.28. 586, 742. 855. 1932,1944 Acetobaclerxylimim. 26. 27. 28. 283. 284. 586, 742, 1474, 1475, 1476. 1950 Acetobacler xylinum Medium. 28 Acetolmcler-Gluconolxtcter Agar, 28 Acetobacierium, 2 Medium, 35 Acetobacierium Autotrophic Medium, 28 Acelabacterium bakii, 35 Acetobacierium carbinolicum, 30 Acetobacierium carbinolicum Medium, 29 Acclofyacterium dehalogenans. 30 Acetobacierium dehalogenans Medium, 30 Acetolyacterium Jimetariitm DSM 8237, 38 Acetobacierium fimelarium DSM 8238, 37 Acetobacierium fnnetarium DSM 8239, 38 Acetoi)ucierium I leterotrophic Medium. 30

1957

Acetobacierium maiicum, 30 Acetobacierium Medium, 31, 32, 33, 34 Acetobacierium paludosum. 35 Acetobacierium sp. KoMAcl Medium, 36 Acetobacierium sp. Medium. 36, 37 Acetobacierium species, 29, 31, 32, 33,34, 36.972.974 AcetolHicterium luiulrae. 38 Acetobacterium tundrae Medium. 38 Acetobacierium woodii. 36,99 Aceiobacteroides glycinophilus, 39 Aceiobacteroides glyx'tnophilus Medium. 38 Acelobacterum wieringae, 99 Acetogen Medium. 39 Acetogenium kivu, 1074 Acetogenium Medium, 40 Acetohalobium arahaticum. 41,42 Acetohalobium Medium. 40,41,42 Acetohalobium species, 1276, 1277 Acetoin production. 1219. 1237.1894 Acetoin utilization, 100 Acetomicrobium faecalis. 43 Acetomicrobium faecalis Medium, 42 Acetomicrobium flavidum, 43, 44, 1459 Acetomicrobium flavidum Agar, 43, 1110 Acetomicrobium flavidum Broth. 43 Acetonema longum, 44 Acelonema Medium, 44 Acetylglucosamine Medium, 44 Achaetomium glohosum, 340 Acholeplasma Medium. 44.45 Acholeplasma monim, 1423 Acholeplasma species, 45, 816. 817, 1424 Achromobacter Choline Medium, 45 Achromobacter Choline Medium. Modified, 45 Achromobacter cholinophagum, 45 Achromobacter hoiinophagum. 365 Achromobacter Medium. 45, 46 Achromobacter methanolophiia. 1111, 1924 Achromobacter pestifer, 46 Achromobacter pestifer Medium. 46 Achromobacter species. 46 Achyla species, 1479 Aciculoconidium aculeatum, 589 Acid Bismuth Yeast Agar, 17 Acid Broth, 46 Acid Egg Medium, 46 Acid Glucose Salts Medium, 46 Acid HiVeg Broth, 46 Acid production. 96. 181, 1236,1237 Acid Products Test Broth. 46 Acid Rhodospirillaceae Medium, 47 Acid tolerant micrwrganisms. 47 Acid Tomato Broth, 47 Acidaminobacter hydrogenoformans, 48 Acidaminobacter Medium. 47 Acidamhuxoccus fermenlans, 48, 49 Acidaminococcus fermenians Medium, 48 Acidamtnococcus Medium VR, 48 Acidianus brierleyi. 49, 50. 1659 Acidianus brierleyi Medium, 49

1958

Index

Acidianus infernus. 49. 50 Acidianus infernus Medium. 49, 50 Acidic Rhodospirillacejic Medium, 51, 54 Acidic Tomato Medium Tor LeuconostOC, 51 Acidicaldns Medium. 50 Acidicaldus organivorans. 51 Acidified Potato Dextrose Agar, 51 Acidilobus aceticus, 53 Acidimicrobinm Medium, 51.52 Acidiphilium acidophilum, 1752 Acidiphiiium angitstum, 951 Acidiphilium cryptum, 52. 54, 951. 1239 Acidiphilium facilis, 951 Acidiphilium Medium, 52 Acidiphilium oi-ganovorum, 390 Acidiphilium rubrum. 951 Acidiphilium species, 54 Acidiphilium ciyplwn DSM, 9467, 1239 Acidisphaera rubrifaciens. 755, 756 Acidithiobacillus caldus. 1238 Acidithiobacillusferrooxidaivi. 946, 1238 Acidohacferium capsulatiim, 52 Acidolnicterium Medium, 52 Acidogcnic microorganisms. 860.905,918 Acidolobus aceticus Medium, 52 Acidomonas Agar, 53 Acidumonas methanolica, 27. 28, 53. 1140 Acidomonas methanolica Agar, 53 Acidophilic Bacillus sfearolhermophtlus Agar, 53 Acidoj)hilic Bacillus stearothennophilus Broth, 53 Acidophilic bacteria, 1005 Acidophilium Agar, 54 Acidolhermus cellulolyticus, 968 Acidowrax avenae, 1304 Acidovorax delajieldii, 1304 Acidovorax facilis, 1304, 1307 Acidowrax konjaci, 1304 Acidowrax species. 1304 Acidowrax temperans, 1304 Acid-producing microorganisms, 1288 Aciduliprofundum Medium, 54 Acidulipmfundum sjxxries, 54 Aciduric. 692,1261 Aciduric bacteria, 762 Aciduric microorganisms, 1329.1330,1410. 1416, 1801, 1934 Acinetobacter baumannii. 1174, 1368 Acinetobacter calcoaceticus, 1561, 1826 Acinetobacter Iwofjii, 197, 1369, 1561 Acinetobacter species. 821,1330, 1824 Acinetobacter tartarogenes, 749 Acremonium chrysogenum, 998 Acremonium sclerotigenum, 348 Acremonium species. 589 Acrocarjxtspora macrocephala. 885 Acrodontium s implexy 589 Acrophialophora fusispora. 999 Actidione HiVeg Agar Base with Actidione*, 55

Actidione HiVeg Agar Base without Actidione* with Antibiotics, 55 Actidione I IiVeg Agar with Actidione*. 54 Actidione*' Agar, 54 Aclimomycclcs. 58 Actinobacillus lignieresii, 56, 808, 1476 Actinobacillus lignieresii Medium, 55 Actinobacillus pleuropneumoniae. 774 Actinobacillus species. 253 Actinobacillus ureae, 306 Actinobaculum species, 441 Aclinobolin Medium, 56 Actinomadura attwnentaria. 1929 Actinomadura aurantiaca, 1330 Actinomadura citrea. 1323 Actinomadura coerulea. 1323 Actinomadura cremea, 1323 Actinomadura echinospora, 743 Actinomadura fastidiosa. 886 Actinomadura ferruginea. 1645 Actinomadura fibrosa, 1319 Actinomadura kijaniala, 1323 Actinomadura libanotica, 1645 Actinomadura livida. 1939 Actinomadura madurae, 210,754,978, 1330, 1469, 1645 Actinomadura pelletieri,2\<\, 1330, 1645 Actinomadura mseoviolacea, 886, 1645 Actinomadura nibrobrunea, 1323, 1363, 1517 Actinomadura species, 159, 320, 321, 444, 752. 767.886.907.1153. 1318.1321. 1862.1877.1915, 1927 .Actinomadura spiralis, 1645 Actinomadura umhrina, 210 Actinomadura verrucososporti. 1645 Actinomadura vinacea. 1323, 1330 Actinomadura viridis, 1323 Actinomucor elegans, I (MM Actinomyces Agar. 56 Actinomyces bavis, 216.254 Actinomyces Broth, 56. 57 Actinomyces denticolens, 1459 Actinomyces georgiae, 215 Actinomyces gerencseriae, 215.216 Actinomyces HiVeg Agar. 57 Actinomyces WiVcg Bmth, 57 Actinomyces hordeovulneris, 253,369, 1459 Actinomyces humifenis. 57 Actinomyces humiferus Medium, 57 Actinomyces hyovaginalis, 253, 1800 Actinomyces Isolation Agar, 57 Actinomyces israelii. 215, 254. 332, 1258 Actinomyces meyeri, 1459 Actinomyces naeslundii, 215,216, 254, 297, 344,718 Actinomyces odontolyticus, 215. 216, 250 Actinomyces pyogenes, 1459 Actinomyces species, 56, 57, 371,440. 441, 895 Actinomyces suis. 366

Actinomyces viscosus, 215, 216. 344, 718. 1175 Actinomycetaccae, 638 Actinomycete Growth Medium, 57 Aclinomycctc Isolation Agar. 58 Actinomycete Isolation I liVeg Agar, 58 Actinomycete species. 1862. 1915 Actinomycetes, 321, 481, 672, 1320, 1321, 1485. 1584.1683. 1934. 1936 Actinoplanaceae, 479 Actinoplanes awajinensis, 753. 1410. 1411 Aclmoplanes caeruleus. 758, 1319 Actinoplanes campanulatus, 481 Actinoplanes digitalis, 481 Actinoplanes italicus, 481 Actinoplanes kinshanensis. 1411 Actinoplanes lobatus, 481 Actinoplanes Medium, 58 Actinoplanes missouriensis, 214, 1645 Actinoplanes nirasakiensis. 1410 Actinoplanes nirasakinensis, 1410, 1411 Actinoplanes philippinensis. 214 Actinoplanes piriformis, 1411 Actinoplanes regularis. 481 Actinoplanes species, 58,321,458,480,767, 886.1318.1320.1321.1323.1411.1934. 1939 Actinoplanes utahensis, 481, 1411 Actinoplanes violaceus, 743 Actinopolyspora halophila, 320, 431 Aclino[)olyspora Medium, 58 Actinopolyspora mortivallis, 431 ActinofMilyspora species. 159,320.444. 907. 1153,1318,1877, 1927 Actinopolyspora thennovinacea. 58 Actinospora megalospora, 1004 Actinosporangium species. 1323 Actinosynnema mirum, 1939 Activated Carbon Medium, 58 Acytostelium ellipticum, 746 Acytostelium subglobosum. 930 Adams Agar, 58 ADA-V. 94 Adelphamoelm galeacystis. 1924 Adonitol Tcrmcnting bacteria. 1385 AE Medium (4a/3e), 59 AH Sporulation Medium, Modified. 59 Aerial hyphae morphology, 1683 Aero Pseudo Selective Agar, 60 Aerobes. 17, 18. 244, 249. 250,447, 588, 645,694,695,732,733,785,1289,1290, 1291.1549.1598.1634.1683.1772. 1761,1772,1774, 1775,1836,1837. 1838. 1839.1845. 1847.1848.1849. 1850 Aerobic actinomycetes, 375, 672, 1683, 1869. 1871 Aerobic Low Peptone Basal Medium, 87 Aerococcus species. 1801 Aerococcus viridans, 744, 920, 1351, 1428 Aewmicrobium erythreum, 1857 Aeromonas DitTerential Agar, 60

Index

Aerumonas encheleia, 674 Aeromonas hydrophila, 60,61.221,645. 916, 1428, 1514, 1520, 1526, 1527 Aeromonas hydrophila Medium. 60 Aeromonas Isolation HiVcg Mcdiuni Base. 61 Aeromonas Isolation Medium. 60 Aeromonas Medium, 61 Aeromonas salmonicida, 682, 1928, 1940 Aerumonas species, 60,61,94,668,877,994, 1484,1514,1515, 1538,1554,1830 Aeropyrum JXT Medium, 61 Aeropyrunt pernix. 61 Afipia broomeae, 471 Afipia clevelandensis, 471 Afipia J'elis, 471 AI-TA, 62 AG Medium, 62 Agar 5g, 682 Agar 6b, 1574 Agar diffusion technique. 901.902 Agar dilution lest. 899. 900 Agar Listeria Ottavani & Agosti, 85, 86 Agar Medium A, 130 Agar Medium C, 131 Agar Medium for Differential Enumeration of I-actic Streptococci, 62 Agar Medium P, 62 Agar streak method, 1948 Agaricus augustus, 999 Agaricus hisporus, 998, 1004 Agaricus macrosf>orus. 775. 1065 Agaricus species. 472 Agaricus xanthoderma, 1065 Agars, 1 Agitococcus Ittbricus. 1855 Agrobacterium Agar. 63 Agrobacterium Agar with Biotin, 63 Agrobacterium azotophilum, 63 Agrolxicterium Mannitol Medium. 63 Agrobacterium Medium. 63.64 Agrobacterium Medium D1, 64 Agrobacterium radiobacter, 63 Agrobacterium rhizogenes, 63 Agrolxicterium rubi, 63 Agrobacterium species. 64. 745, 746. 748. 852 Agrobacterium species biotype, 2, 63 Agrobacterium fume/aciens. 63, 1310. 1314. 1669 Agrobacterium tumefaciens biovar 1, 65 Agrobacterium tumefaciens biovar 2, 65 Agrobacterium tumefaciens biovar 3, 65 Agrobacterium tumefaciens Modified Roy and Sasser Medium for Grapevine Strains. 64 Agrobacterium tumefaciens Selective Medium, 65 Agrohacterium viscosum, 749 Agrohacterium vitis, 63 Agrocybe aegerita. 998

Agromonas oligotrophia. 1305. 1306.1314, 1905 Agromyces ramosus. 214 Agromyces species. 1830 AGS. 146 AHS Medium, 65 Air. 192,193,657 AJYK Medium, 136 AK Agar No. 2.66 AKI Medium, 66 Albidovtdum inexpec/atum, 1219 Albumin Patty Acid Broth, Leptospira Medium. 240 Albumin Fatty Acid Semisolid Medium, Modified. 240 Aleal Mannose Medium, 66 Alcaligenes Agar, 67 Alcaligenes eutrophus. 1177,1340, 1953 Alcaligenes faecalis. 67. 68, 470. 1561 Alcaligenes latus, 1176.1340.1368, 1916 Alcaligenes Medium, 67 Alcaligenes metalcaligenes. 1885 Alcaligenes N5 Medium. 67 Alcaligenes NA YE Medium. 67 Alcaligenes NB YF Agar, 68 Alcaligenes NB YF Broth, 68 Alcaligenes NB YF Medium, 68 Alcaligenes Nutrient Agar Yeast Fxtract Medium, 67 Alcaligenes Nutrient Broth Yeast Fxtract Agar, 68 Alcaligenes Nutrient Broth Yeast Fxtract Broth. 68 Alcaligenes Nutrient Broth Yeast Fxtract Medium. 68 Alcaligenes species, 46,67, 68, 206, 354, 1039.1044,1173,1333 Alcaligenes tolerans, 67 Alcaligenes xylosoxydans, 68, 212, 1340 Alcaligenes xylosoxyxians Medium with Bcnzoate, 68 Alcaltphilic Amphihacillus Strains Medium, 68,798 Alcanivorax borkumensis, 69 Alcanivorax borkumensis Medium, 69 Alcanivorax jadensis. 711 Alcohol utilization. 87 Alcoholic mash, 1911 Algae, 69,739 Algae Culture Broth, 69 Algal Proteose Agar. 69 Alginate Utilization Medium, 70 Alicyclohacillus acidocaldarius, 175 Alicyclolxtcillus acidoterrestris. 71. 176, 179 Alicyclolxtcillus acidoterrestris Agar. 70 Alicyclobacillus acidoterrestris Broth, 71 Alicyclohacillus acidoterrestris Medium, 71 Alicyclobacillus Agar, 71 Alicyclobacillus cycloheptanicus. 72, 73. 176,179 Alicyclolxtcillus cycloheptanicus Agar, 72

1959

Alicyclobacillus cycloheptanicus Medium. 72 A licyclobacillus ferrooxydans. 73 Alicyclohacillus fetrooxydans Medium, 72 Alicyclobacillus Medium, 73 Alicyclohacillus species, 72, 73 Aliphatic acid utilization. 87 Alkalibaclerium olivapovliticus, 74 Alkalibaclerium olivapovliticus Agar. 73 Alkalibaclerium olivapovliticus Medium, 74 AlkaliJIexus Medium. 74 Alkaliflexus species, 74 Alkaline Bacillus Medium, 74 Alkaline Cellulose Agar, 75 Alkaline Hi Veg Peptone Water, 75 Alkaline Nutrient Agar, 75 Alkaline Peptone Agar, 75 Alkaline Peptone Salt Broth, 75 Alkaline Peptone Water, 76 Alkaline Polypcctatc Agar. 76 Alkaline Starch Agar, 76 Alkaline Xylan Agar, 76 Alkaline Xylan Broth, 76 Alkaline Xylan Medium, 77 Alkaline Yeast Extract Malt Medium, 77 Alkaliphilic bacteria. 911 Alkahphilic Halomonas Medium,, 77 Alkaliphilic Mclhanogcn Medium, 77 Alkaliphilic Spirochete Medium, 78 Alkaliphilic Sulphur Respiring Strains Medium. 78. 79. 80, 81 Alkaliphilic Thermoeoccw Medium, 82 Alkalophile Medium. 83 Alkaloplulic, 74 Alkalophilic Halophilc Agar, 83 Alkaloplulic I lalophile Broth, 83 Alkaloplulic microorganisms. 76 Alkilophilic bacteria, 1307, 1308 Alkvisco Medium. 84 Allantoin Agar, 84 Allantoin Broth, 84 Allantoin Mineral Medium, 84 Allen and Anion Medium with Nitrate, 84 Allisonella Medium. 85 Allisonella species, 85 Allochromatium remtkae. 1497 Atlomyces javanicus, 1952 Allomyxes species. 638 Almond Curd Agar, 85 ALOA Medium. 85.86 AFP Basal Medium, 87 ALP Basal Medium Low pll. 87 Altereiythrobacler iixliais, 1558 Allernaria ailernata. 1410 Allernaria brassicae. 1410 Allernaria citri, 1410 Allernaria dianthi. 1410 A Iternaria dianthicola, 1410 Allernaria porri. 809 Allernaria radicina. 1410 Allernaria solani. 1410 Allernaria species, 809

1960

Index

Altermiria tenuissima. 1410 Alternative Thioglycollate Medium, 87 Alteromonas atlantica. 1009 Alleromonas communis, 1025 Alleromonas denitrijicans, 88. 1514 Alleromonas denitrijicans Medium, 87 Alteromonas espejiana. 95. 1558 Alteromonas haloplanktis, 95, 1018, 1441 Alteromonas hanedai. 971. 1395 Alteromonas Inteoviolacea, 384 Alteromonas macleodii. 95 Alleromonas Medium, 88 Alteromonas nigrifaciens. 95. 1311, 1441 Alleromonas rubra, 95, 1554 Alteromonas species. 88. 611. 1017. 1018, 1557, 1893 Alleromonas vaga, 1025 Alysiella filifonnis. 1309 Alysiella species, 279, 1827 Amastigomonas bermudensis, 1556 Amastigomonas species, 1015 Amauroascns species, 706 AMB Agar, 88 AMB Broth, 88 AMB Medium, 88 American Association of Textile Chemists and Colorists Bacteriostasis Agar. 16. 670 American Association of Textile Chemists and Colorists Bacteriostasis Broth, 670 American Association of Textile Chemists and Colorists Mineral Salts lion Agar, 16 American Society for Testing and Materials Nutrient Salts Agar, 155 American Trudeau Society Medium, 157 AMI 1,88 Amies Modified Transport Medium with Charcoal, 89 Amies Transport Medium without Charcoal, 90 Aminiphilus Medium, 90 Aminiphilus species, 91 Amino Acid Assay Medium, 91 Amino acid decarboxylation. 487 Aminobacter aminowrans, 435, 444. 1304 Aminobutyric Acid Medium, 91 Aminomonas aminovonts, 435 Aminophcnol-utili/ing bacteria. 1171 Ammonia-oxidizing bacteria, 1040. 1041 Ammomfex Medium, 91 Ammonifex species. 92 Ammoniphilus oxalaticus, 1327 Ammoniphilus axaiiwrans, 1327 Ammonium compounds, 947, 948 Ammonium Mineral Salts Agar. 95 Ammonium Mineral Salts Agar without Methanol. 95 Ammonium Phospliate Agar, 92 Ammonium Yeast l-.xtract Medium. 182 AMO.1 Medium, 92 Amoebae species. 601 Amoebidium jxiratiticum, 477, 1146, 1839

Amoebobacter Medium. 93 Amoebohacter pedioformis, 93 Amoebobacter pendens. 1378 Amoebobacter purpureus, 93 Amorphosporangium auranticolor, 480, 1410, 1411 Amorphotheca resinae. 944 Amphibacillus Jermentum, 69 Amphibacillus Medium. 93 Amphibacillus tropicus, 69 Amphibacillus xylaniis, 76, 77, 93 Amphidinium carleri. 599 Amphiprora hyalina, 822 Amphora rocttgeri. 1589 Ampicillin IX'Xtrin Agar, 93 Ampicillin Dextrin Agar with Vancomycin, 94 Ampicillin Kanamycin Nutrient Agar. 94 Ampicillin I. Broth Medium, 94 Ampicillin Selective Supplement, 6 Ampicillin TY Salt Medium, 95 AmpullarieJla campanulata, 481, 1159 Ampuilariella kummingensis, 743 Ampullariella species, 480 AMS Agar. 95 AMS Agar without Methanol, 95 AMS Medium, 95 AMS Medium, Modified. 95 Amycolata autotrophics. 743. 754. 767,1936 Amycolala hydrocarhonoxydans, 1939 Amycolata saturnea. 754. 1309 Amycolalopsis faslidiosa, 1318 Amycolalopsis kentuckyensis. 1319 Amycolalopsis mediterranei, 886, 1330, 1939 Amycolalopsis methanolica. 743 Amycolalopsis orientalis, 767, 1322, 1330. 1936, 1939 Amycolalopsis orientalis subsp. Orientalis, 768 Amycolalopsis pretoriensis. 1319 Amycolalopsis rugosa, 743, 1637 Amycolalopsis sulphurea. 743, 767, 1330 Amygdalin hydrolysis, % Amygdalin Medium. 95 Amylase production. 181, 1634, 1636 Amylostereum laevigatum, 348 AN1 Medium, 96 AnabaenaJJosaquae. 758 Anabaena species, 85, 213, 214, 388, 1296 Anabaena variabilis, 214 Anacker and Ordal Medium, 96 Anacker and Ordal Medium, Knriched, 96 Anacker-Ordal Agar, 96 Anaerobacter Medium, 96 Anaerolxicter polyendosporus, 97 Anaerobaculum thermoterrenum, 97 Anaerobaculum thermoterrenum Medium, 97 Anaerobe Anaerobe Anaerobe Anaerobe

Agar. 97.98 Medium. 98 Medium No. 1.99 Selcective Supplement (IN, 6

Anaerobe Selective Supplement NS, 6 Anaerobes. 17, 18,40. 98, 269, 270, 366, 466. 666, 667. 700. 892. 1486, 1487, 1593, 1658, 1714, 1730,1772, 1843, 1905. 1906.1907 Anaerobic Acetoin Medium, 99 Anaerobic Agar, 100 Anaerobic Agar without Dextrose, 101 Anaerobic Agar. Brewer. 100 Anaerobic bacteria, 56,57,98,99, 100,103, 107.108. 244.249.250.256.269. 332. 333, 334, 358, 365, 367,369, 371. 389, 447. 448, 461, 632, 633,645, 672, 693, 694.695. 732. 733.880.881.939.959. 961. 963. 964, 965. 1034, 1035, 1226. 1427,1450,1457, 1477, 1549, 1550, 1598. 1760,1772. 1774.1775, 1804, 1830, 1871, 1873, 1874,1905, 1906, 1907 Anaerobic Basal Agar with Blood, 101 Anaerobic Blood Agar Base with Blood and Neomycin, 101 Anaerobic Broth, 101 Anaerobic Cellulolytic Medium, 101 Anaerobic Cholesterol Medium, 102 Anaerobic Citrate Medium, 103 Anaerobic CNA Agar, 103 Anaerobic CNA Agar Base with Blood. 103 Anaerobic Colistin Nalidixic Acid Agar. 103 Anaerobic o-Ciluconate Medium, 105 Anaerobic l\gg Yolk Agar, 104 Anaerobic ligg Yolk Base with Kgg Yolk I'juulsjon, 104 Anaerobic Glucuronic Acid Medium. 105 Anaerobic HiVeg Agar, 105 Anaerobic HiVeg Agar (Brewer). 105 Anaerobic HiVeg Agar Base with I-gg Yolk Emulsion. 105 Anaerobic HiVeg Agar without Dextrose, 106 Anaerobic 1 li Vcg Agar witlioui Dextrose and Eh Indicator, 106 Anaerobic indicator, 1328 Anaerobic I.KV Blood Agar, 106 Anaerobic microorganisms, 100. 101, 105, 106.960 Anaerobic Oxalate Medium, 106 Anaerobic streptococci, 104 Anaerobic 'Hiioglycollate Medium Base with Serum. 107 Anaerobic TrypticascIM Soy Agar with Calf Blood, 107 Anaerobic TrypticascIM Soy Medium with Calf Blood, 107 Anaerobic Tryptonc Soya Agar. 108 Anaerobic TVLS Medium, 108 AnacrobiospiriUum suceiniciproducens. 366. 1459 Anaembiospirillum ihomasii. 108 Anaembiospirdlum thomasii Medium, 108 Anaerobranca gotlschalkii, 109 Anaerobranca gottschalkii Medium, 108

Index

Anaerobranca horikoshii. 110 Anaerobranca Medium, 109 Anaerocellum Medium, 110 Anaerocellum ihermaphilum, 110, 111 Anaerofilum agile. 111 Anaerofilum Medium, 111 Anaerofilum pentosovorans. 111 Anaerolinea Medium, 111 Anaerolinea Medium with Sucrose, 112 Anaerolinea Medium without Glucose, 113 Anaemlinea species. 112 Anaerolinea thermoitmosu, 113 Anaeromyxobacter Medium, 114 Anaeromyxobacter species, 114 Anaerospirillum Medium. 114 Anaerospirillum succiniciproducens, 115 Anaerovihrio hurkinabensist 115 Anaerovibrio bitrkinabensis Medium, 115 Anaerovibrio glycerini, 1487, 1522 Anaerovibrio lipolytica. 1419. 1522 Anaplychia cilaris, 951 Ancalomicrobium adetum. 116. 1058. 1059 Ancalomicrobium adetum Medium, 115 Ancalomicrobium Medium, 116 Ancalomicrobium species, 1057, 1058 Ancylobacter aquations, 116, 320, 740. 741, 1838 Ancylobacter species, 116, 827, 828, 1111 Ancylobacter Spirosoma Agar, 116 Ancylobacter Spirosoma Medium, 116 Ancyromonas sigmoides. 1556 Andersen's Pork Pea Agar, 117 Anderson's Marine Agar, 117 Anderson's Marine Drum, 117 Anderson's Marine Medium. 117 Andrade, 118 Andrade HiVcg Peptone Water, 118 Andrade Peptone Water with HiVeg lixtract No. 1.118 Andrade Peptone Water with Meat Extract, 118 Andradcs Broth. 118. 119 Aneurinibacillus aneurinilylicus, 1830 Angiococcus disciformis, 1367 Angulomicrohium letraedrale, 1 198 Animal feces. 1274 Animal feed, 630, 631 Animal tissue culture. 619.620.621 Animal tissue culture cells, 145 Anisoin Minimal Medium, 119 Ankistrotiesmus augustus. 70 Ankistradesmus hraunii, 70 AN()2 Fungus 11, 1282 Anophryoides soldoi, 989 Anophryoides species, 226 Anaxyhacillus amylalyticus, 119 Anoxybacillus amylalyticus Medium.. 119 Anaxyhacillus Medium, 119 Anoxybacillus pushchinoensis. 120 Anoxybacillus winowkiensis, 1218 Anoyxnatronum Medium., 120 Anoxynalnmum sj)ecies, 121

Antarctobacter heliothermus, 635 Anihracis Chromogenic Agar, 121 Anthracothecium albescens. 951 Anthranilic Acid Medium, Revised, 121 Antibacterial activity, 16 Antibacterial testing, 670 Antibiotic, 66. 1564. 1645 Antibiotic activity assay, 1645 Antibiotic Assay Medium B, 125 Antibiotic Assay Medium C, 125 Antibiotic Assay Medium I). 125 Antibiotic Assay Medium E, 126 Antibiotic Assay Medium F, 126 Antibiotic Assay Medium 0 , 126 Antibiotic Assay Medium II. 126 Antibiotic Assay Medium J-HiVeg, 1841 Antibiotic Assay Medium L-AODC, 126 Antibiotic Assay Medium M-AOIXJ, 126 Antibiotic Assay Medium No. 1,121 Antibiotic Assay Medium No. 2.122 Antibiotic Assay Medium No. 3,122 Antibiotic Assay Medium No. 4.122 Antibiotic Assay Medium No. 5, 122 Antibiotic Assay Medium No. 6.122 Antibiotic Assay Medium No. 8,122 Antibiotic Assay Medium No. 9.123 Antibiotic Assay Medium No. 10,123 Antibiotic Assay Medium No. 11, 123 Antibiotic Assay Medium No. 12.123 Antibiotic Assay Medium No. 13, 123 Antibiotic Assay Medium No. 19,123 Antibiotic Assay Medium No. 20, 124 Antibiotic Assay Medium No. 32.124 Antibiotic Assay Medium No. 34, 124 Antibiotic Assay Medium No. 35.124 Antibiotic Assay Medium No. 36, 124 Antibiotic Assay Medium No. 37. 124 Antibiotic Assay Medium No. 38.124 Antibiotic Assay Medium No. 39.125 Antibiotic Assay Medium No. 40, 125 Antibiotic Assay Medium No. 41.125 Antibiotic Assay No 37, HiVeg, 1842 Antibiotic assay testing, 122, 123, 124, 126, 127,128. 129,130. 131,132,1933 Antibiotic HiVeg Assay Medium - A, 126 Antibiotic HiVcg Assay Medium - B. 126 Antibiotic HiVeg Assay Medium - C, 127 Antibiotic HiVcg Assay Medium - E. 127 Antibiotic HiVeg Assay Medium - 1 . 129 Antibiotic HiVeg Assay Medium - J, 129 Antibiotic HiVeg Assay Medium F, 127 Antibiotic HiVcg Assay Medium G. 128 Antibiotic HiVeg Assay Medium H, 128 Antibiotic HiVcg Assay Medium No. 1, 126 Antibiotic HiVeg Assay Medium No. 2, 126 Antibiotic HiVeg Assay Medium No. 3, 127 Antibiotic HiVeg Assay Medium No. 4.127, 1933 Antibiotic Antibiotic Antibiotic Antibiotic

HiVcg Assay HiVeg Assay HiVcg Assay HiVeg Assay

Medium Medium Medium Medium

No. 5. No. 6, No. 8, No. 9,

127 127 127 127

1961

Antibiotic HiVeg Assay Medium No. 10, 128 Antibiotic HiVeg Assay Medium No.l 1, 128 Antibiotic HiVcg Assay Medium No. 12, 128 Antibiotic HiVeg Assay Medium No. 13, 128 Antibiotic HiVcg Assay Medium No. 19, 128 Antibiotic HiVeg Assay Medium No. 20,128 Antibiotic HiVeg Assay Medium No. 32.129 Antibiotic HiVeg Assay Medium No. 35,129 Antibiotic I liVcg Assay Medium No. 36.129 Antibiotic HiVeg Assay Medium No. 37,129 Antibiotic I liVeg Assay Medium No. 38.129 Antibiotic I liVeg Assay Medium No. 39.129 Antibiotic HiVcg Assay Medium No. 40.130 Antibiotic HiVeg Assay Medium No. 41,130 Antibiotic Medium 1, 130 Antibiotic Medium 1 with Tetracycline, 130 Antibiotic Medium 2, 131 Antibiotic Medium 3, 131 Antibiotic Medium 3 Plus, 131 Antibiotic Medium 4, 131 Antibiotic Medium 5, 131 Antibiotic Medium 6, 131 Antibiotic Medium 7, 131 Antibiotic Medium 8. 132 Antibiotic Medium 9, 132 Antibiotic Medium 10. 132 Antibiotic Medium 11, 132 Antibiotic Medium 12.132 Antibiotic Medium 13, 132 Antibiotic Medium 19. 133 Antibiotic Medium 20,133 Antibiotic Medium 21, 133 Antibiotic production. 1645 Antibiotic Sulfonamide Sensitivity Test Agar, 133 Antibiotics. 123.128.133. 1258 Antifungal. 858 Antifungal agents, 322 Antifungal Assay Agar. 133 Antifungal Assay HiVeg Agar, 133 Antimicrobial disc diffusion susceptibility testing, 1250 Antimicrobial Inhibitor Test AgarpH 6.0, 134 Antimicrobial Inhibitor lest AgarpH 7.2,134 Antimicrobial Inhibitor Test AgarpH 8.0.134 Antimicrobial susceptibility, 847,848, 1159, 1160.1161.1162.1163.1167

Antimicrobial susceptibility (MIC) testing, 1906 Antimicrobial susceptibility testing, 882, 1248.1249 Antimicrobial susceptibility tests, 244, 322 Antimicrobial testing, 590 Antimicrobic susceptibility testing, 1905. 1906, 1907 Antimicroibal sensitivity testing, 1564 Antimycin Medium, 134 Antimycolic Sensitivity Test Agar. 134 Antiseptics, 486, 590. 670 Anti-tubercular drugs, 1164. 1165 Antrodia serialis, 1002, 1065

1962

Index

AC) Agar, 135 AOAC Bacillus steamthennophilus Qualitative Disc Method II. 1405 AOAC I.etheen Broth, 135 Aolpha Medium. 135 APDA,51 Aphanoascus cinnabarinus. 706 Aphanomyces sjxxies, 136 Aphanomyccs Synthetic Medium. 136 Apiosordaria rotttla, 944 Aplanobacterium Medium. 136 Apple Juice Yeast Lxtract Medium, 136 APRY Agar, 136 APRY Agar Base, 146 APRY Agar Base with Acetic Acid and Sorbatc. 136 APRY Broth, 137 APRY Broth Base with Chloramphenicol, 137 APS Broth. 75 API Agar, 137 APT Broth, 138 API HiVeg Agar. 138 APT HiVeg Broth, 138 Aquabacter spiritensis, 139, 1198 Aquabacter spiritetviis Medium. 139 Aquabacter species, 1666 Aquaspirillum anulus, 1366 AqtiaspiriHum arcticum. 826, 829 Aquaspirillum Autotrophic Agar, 139 Aquaspirillum Autotrophic Broth, 139 Aquaspirillum autolrophicum, 139, 140, 1602 Aquaspirillum bengal, 1366 Aquaspirillum dispar, 1366, 1602 Aquaspirillum fasciculus, 1381 Aquaspirillum gracile, 1601 Aquaspirillum Heterotrophic Agar. 140 Aquaspirillum Heterotrophic Broth, 140 Aquaspirillum ilersonii. 1370 Aquaspirillum magnetotacticum, 995, 996, 997 Aquaspirillum Medium, 140 Aquaspirillum metamorphum, 116 Aquaspirillum peregrinum, 1370, 1602 Aquaspirillum peregrinum subsp. integrum. 1370 Aquaspirillum psychrophilum, 1370 Aquaspirillum serpens. 116, 1366, 1602 Aquaspirillum species. 141, 827, 828 Aquifer pyrophilus, 1581. 1582 Aquincola Medium,, 141 Aquincola species. 141 Aquisalimonas Agar, 141 Aquisalimonas Medium. 141 Aquisalimonas species, 141 Arabinosc Agar Base with Selective Supplement, 141 Arabinosc fermentation. 1565 Arabinosc fermenting bacteria. 1385 Arachnia propionica, 215 A rachniofus Jla voluteus, 706

Arachniotus bebridensis. 706 Arachnotheca albicans, 706 Arachnollteca glomerata. 944 ARC51 Medium, 141 Arcanobaclerium haemolyticum, 1459 Arcanobacterium species, 440 Archaeoglobus fulgidus. 143 Archaeoglohus in/ectus, 142 Archaeoglobus Medium. 142 Archaeoglobus profundus, 143 Archaeoglobus profundus Medium, 143 Archaeoglobus veneftais. 144 Archaeoglobus veneficus Medium, 143 Archangium primigenium, 1272 Archangium violaceum Medium, 144 Archanigium gephyra, 1594 Archgium violaceum, 144 Arcolxicter Broth Base with Selective Supplement, 144 Arcobacter Medium. 144 Arcobacler nilrojigilis, 144 Arcobacter nitrofigilis Agar. 144 A rcobacler species, 144, 1249 Arenariomyves triseptatus, 287 Arenavirus Plaquing Medium, 144 Arenaviruses. 145 Arginine Broth. 145 Arginine Broth with Sodium Chloride, 145 Arginine decarboxylation. 145, 146 Arginine dihydrolase, 598, 1220 Arginine Dihydrolase Broth. 145 Arginine Dihydrolase Hi Veg Broth, 145 Arginine Dihydrolase Medium, Modified, 146 Arginine dihydrolase production, 1040 Arginine Glucose Slants, 146 Arginine utilization, 62 Arhodumonas aquaeolei, 146, 298 Arhodomonas Medium, 146 Arizona species, 1700. 1701 Armillaria mellea, 998, 1065 Armstrong b'usarium Medium. 146 Arsenophonus nasoniae, 215, 724 Arthonia cinnabarina, 951 Arthrinium phaeospennum, 1001 Arthrobacter atroeyaneus. 147 Arthrohacter aurescens, 147 Arthrobacter Broth. 147 Arthrobacter crystallopoietes. 147 Arthrobacter dextranyliticum, 584 Arthrobacter globiformis. 147,348. 1428 Arthrobacter hislidinolovorans, 147 Arthrolxtcter ilicis, 324 Arthrobacter Medium, 147 Arthrobacter mysorens. 1271 Arthrobacter nicontinovorans, 454 Arthrobacter nicotianae. 454 Arthrobacter oxydans, 147,454 Arthrobacter pascens. 454 Arthrobacterpicolinophilus. 1045 Arthrobacter polychromogenes. 454. 1470 Arthrobacter protophonnaie, 1470

Arthrobacter sideroeapsulatus, 1307 Arthrobacter species. 147, 592. 638,665. 774, 814, 1150, 1170, 1171,1179, 1309,

1363.1365.1367, 1428.1584. 1586. 1587, 1815,1881 Arthrobacter sulfureus, 454 Arthrobacter uraloxydans, 454 Arthrobacter ureafaciens. 454 Atihrobacterviscosus.4S'\, 1003. 1934 A rlhrobacter YCWD, 147 Arlhrvbofrys arlhrobotryoides, 1004 Arihroderma benhamiae, 1529. 1532 Arihroderma metis. 206 Arihroderma otae, 1289 Arihroderma vanbreuseghemit, 1289, 1529, 1532 Artilical Organic Lake Medium, 797 Artilical Seawater Medium, 148 Artilical Scawatcr Medium with Propionate. 149 Artificial Deep Lake Medium, 147 Artificial Organic Lake Peptone Medium, 148 Artificial Scawatcr Medium, 155 Arylsulfatase Agar, 150 Ascobolus immersus, 1003 Ascochyta fabae, 1322 Ascochyta pinodes, 1322, 1350 Ascochyta pisi. 1322. 1350 Ascochyta punctata, 1322 Ascochyta viciaepannonicae, 1322 Ascochyta viciaevillosae. 1322 Ascosphaera apis, 1004, 1258 Ascosphaera osmophila, 988 Ascospore Agar. 150 Ascosporcs. 151,701. 1927 \scosporogenous yeasts, I 51 Ascotricha erinacea, 944 Ashby's Glucose Agar, 151 Ashby's Mannitol Agar. 151 Ashby's Nitrogen-Free Agar, 151 Ashdown's Medium, 151 ASLAAgar, 151 ASM Medium. 151 ASN-IIIAgar, 152 ASN-IH Broth, 152 ASP-2 Medium, 152 Asparaginate Glycerol Agar. 153 Asparagine Broth, 153 Asparaginc Gelatin Lactate Medium Base with Lactate. 153 Asparagine Nitrate Medium, 154 Asparagine Proline Broth. 154 Aspergillus aeneus, 1000 Aspergillus amstelodami, 154, 1003 Aspergillus awamori, 154, 1407, 1635 Aspergillus brunneus, 480 Asf>ergiflus caesiellus. 1414 Aspergillus candidus, 1000 Aspergillus clavatus, 1001, 1003 Aspergillus Differential Medium. 154

Index

Aspergillus Differentiation Medium Base with Chloramphenicol, 154 Aspergillus echinulatus. 479 Aspergillus equitis, 480 Aspergillus Jlavipes, 479 Aspergillusflavus, 62, 154 Aspergillus jlavus/parasiticus Agar, 62 Aspergillus fumigatus, 1001 Aspergillus gracilis. 1414 Aspergillus halophiiicus, 988 Aspergillus hollandicus. 480 Aspergillus japonicus. 267 Aspergillus mangini, 1414 Aspergillus Medium. 154 Aspergillus montevidensis, 1414 Aspergillus nidulans. 154,155, 767,1407 Aspergillus nidulans Minimal Medium, 154 Aspergillus nidulellus. 480 Aspergillus niger, 16, 482, 843 Aspergillus niveus. 1414 Aspergillus oryzae, 1438, 1915 Aspergillus parasiticus, 62 Aspergillus penicilloides, 479, 988, 1000, 1414, 1935

Aspergillus repens, 480,1414 Aspergillus reptans, 480 Aspergillus restictus. 988

Aspergillus ruber, 1414 Aspergillus rubrobrunneus, 480 Aspergillus sojae, 267 Aspergillus species. 154,988.998.1002. 1313, 1667 Aspergillus sulphureus, 1414 Aspergillus TeA Medium, 155 Aspergillus tonophilus. 988 Aspergillus versicolor, 1414 Aspergillus vitricolae. 1257 ASS Agar, 133 Assay Broth, 122 Assay testing, 365.465,663,697,698. 699, 700,876,877,899,900,920,1155,1156, 1258. 1288 Association of Official Analytical Chemists Lclhccn Broth. 135 Astasia longa. 860 Asticcacaulis benewsiitus, 1950 Asticcacaulis excentricus. 330 Asticcacaulis Medium. 155 Asticcacaulis species, 155 ASTM Nutrient Salts Agar, 155 ASW Medium, 155 AT5N Medium, 156 A IB Acid Tomato Broth. 156 ATCC Strain 13949, 347 ATCC Strain 1413. 1384 ATCC Strain 21081, 762 ATCC Strain 21588,1953 ATCC Strain 25589,1749 ATCC Strain 27042.632 ATCC Strain 27134,917 ATCC Strain 27136,917 ATCC Strain 31205,1826

ATCC Strain 31384, 364 ATCC Strain 33107, 888 ATCC Strain 35944. 357 ATCC Strain 43554, 1012 ATCC Strain 43554, 1010 ATCC Strain 43826. 604 ATCC Strain 49538, 1571 Atlas Oil Agar, 156 Atopobium minutum, 225. 718.1459 A lopohium pamilum, 1459 Atopobium rimae, 157. 1459. 1460 Atopobium/Olsenella Medium. 156 Atrazinc utilizing bacteria, 1172 ATS Medium, 157 Auerobacterium species. 1312 Aureobacterium acetylicum, 454 Aureobacterium Agar, 157 Aureobacterium arabinogalacfanolyticum. 157 Aureobacterium barkeri, 454 Aureobacterium esteraromaticum, 157 Aureobacterium flax'escens, 147, 1587 Aureobacterium keralanolyticum, 157 Aureobacterium liquefaciens, 454 Aureobacterium saperdae, 454 Aureobacterium schleiferi. 157 Aureobacterium species, 1470 Aureobacterium terrae, 157 Aureobacterium lerregens, 157. 1587 Aureobacterium lerregens Medium, 157 Aureobacterium testaceum, 1334 Aureobacterium trichothecenolyticum, 157 Aureobasidium pullulans, 998. 1532 Aureomycin* Rose Bengal Glucose Peptone Agar. 157 Aureus Agar Base, HiCromeâ&#x201E;˘, 832 A uricularia fiiscosuccinea. 1065 Autoclave sterilization, 8,1611 Autotrophic Nitrobacler Medium, 158 Autotrophic Mitrobacfer species, 158 Auxanographic method, 1290 Auxarlhnm pseudauxarthron, 944 Auxarthron thaxteri, 706 Auxarthnm zuffianum, 706 AUY, 158 AV. 16 Medium. 159 AV Agar with Vitamins, 158 Avian Mycoplasma Agar. 159 Avian Mycoplasma Broth, 160 Avian mycoplasmas, 1266 Axenic Dimastigella Medium, 160 Aycrs and Johnson Agar. 161 Azide Agar, 649 Azide Blood Agar, 162 Azide Blood Agar Base with Blood, 162 Azide Blood Agar Base, HiVeg with Blood, 162 Azide Blood Agar with Crystal Violet, 162 Azide Broth. 162 Azide Broth. Rothc, 163 Azide Citrate Broth. 163 Azide Dextrose Broth, 162

1963

Azide Dextrose Broth, Rothe, 163 Azide IX-xtrose HiVeg Broth, 163 Azide Glucose Broth, 162 Azide Glucose Broth, Rothe, 163 Azidc Medium. 163 Azoarcus indigens, 164 Azoarcus Medium, 164 Azoarcus species. 829 Azoarcus tolulyticus. 1473 Azoarcus VM Medium, 164 Azomonas agilis. 169.1296. 1297 Azomonas tttStgrtts, 1296, 1910 Azomonas macmcytogenes, 170, 12% Azomonas species. 167, 169, 170, 592 Azonexus fungiphilus, 164 Azorhizobium caulinodans. 164 Azorhizobium caulinodans Agar, 164 Azorhizobium doebereinerae, 1856 Azorhizobium species, 1936 Azorhizophilus paspali, 164, 166, 167, 592, 726, 1296 Azorhizophilus paspali Agar, 164 Azospira oryzae. 164, 1045 AzospiriUum amazonense, 165 AzospiriUum amazonense Medium. 164 AzospiriUum brasi/ense, 1602 AzospiriUum halopraeferens, 1672 AzospiriUum lipoferum. 165.235. 1602 AzospiriUum lipoferum Agar Medium, 165 AzospiriUum lipoferum Medium. 165 AzospiriUum Medium, 165 AzospiriUum Medium with 0.17% Agar, 166 AzospiriUum species, 165, 166, 827, 828 Azotobacier Agar. 166 Azotobacier Agar (Glucose), 166 Azotobacter Agar (Mannitol). 166 Azotobacter Agar, Modified I, 166 Azotobacter Agar, Modified II, 167 Azotobacter Basal Agar, 167 Azotobacter Basal Broth, 167 Azotobacter beijerinckii. 167. 1296.1689 Azotobacier Broth, 167 Azotobacter Broth, Modified 1.167 Azotobacter Broth. Modified II. 168 Azotobacter chroococcum, 167, 168, 169, 1296, 1297 Azotobacter chroococcum Agar, 168 Azotobacter chroococcum Medium, 168. 170 Azotobacter Medium, 168, 169 Azotobacter jxisfxili, 169 Azotobacter paspali Medium, 169 Azotobacier species. 101,151,166,167.168. 169, 170.592,746. 1180.1689 Azotobacter Supplement, 169, 170 Azotobacter vinelamlii. 167, 170.284,671, 12%, 1297 Azotobacter vinelandii Medium. 168. 170 Azotrvbacler species, 285

B B Broth, 170,171 B.D.G. Broth,!Iajna,205

1964

Index

B.Q. Vaccine Medium IliVeg with Glucose. 242 B.Q. Vaccine Medium with Glucose. 242 B I B . Inclose Agar, Modified, 280, 928 B.T.B. lactose HiVcg Agar. 280 B.T.B. Lactose IliVeg Agar, Modified, 280, 928 B/lt, 7 A Medium, 171 B ] 2 Assay 1 liVcg Medium. 171 B|2 Assay Medium, 171 B | 2 Culture Agar. USP, 172 B, 2 Inoculum Broth, USP, 172 B I 2 Medium, 172 BA Medium. 172 BA Medium with Ccllobiosc. 172. 187 BA Medium witli Cellulose, 173 Baar's Medium lor Sulfate Reducers, 174 Boar's Medium lor Sulfate Reducers. Modified, 174 Baar's Medium for Sulfate Reducers. Modified with 2.5% Sodium Chloride, 174 liacidia incompta. 951 Bacilli, 696. 1164,1165 Bacillus acidocaldarius, 175, 176, 177 Bacillus acidocaldarius Agar, 175 Bacillus acidoierrestris, 176, 195 Bacillus acidoierrestris Agar, 175 Bacillus acidoierrestris Broth, 176 Bacillus Agar. 176 Bacillus Agar. 1/4 Strength. 176 Bacillus Agar. Modified, 176 Bacillus alcalophilus, 74.75.83. 1304, 1307, 1365 Bacillus alginolyticus, 1314 Bacillus aminovorans. 1617 Bacillus amyloliquefaciens, 1611 Bacillus aruhracis. 121.811. 905. 1313. 1405, 1471 Bacillus arseniciselenatis, 186 Bacillus azotoformans, 1930 Bacillus benzoevorans, 177, 1173 Bacillus benzoevorans Agar, 176, 177 Bacillus brevis, 1454

Bacillus Broth, 177 Bacillus Broth, 1/4 Strength, 177 Bacillus cereus, 121,178,179,200,257,718, 811.812,814. 832.898.899. 978. 1006. 1009.1216. 1224.1303.1330. 1406. 1525,1835, 1893 Bacillus cereui Agar Base with Kgg Yolk Emulsion and Polymyxin, 177 Bacillus cereui IliCromc1*' Agar, 832 Bacillus cereus IliVeg Agar Base with Kgg Yolk l-mulsion, 178 Bacillus cereus Medium. 178 Bacillus cereui Motility Medium, 200 Bacillus cereus Selective Agar Base, 178 Bacillus cereus Selective Supplement, 6 Bacillus chondroitinus, 1314 Bacillus circidans, 67, 74, 83, 812, 1316. 1940 Bacillus cirroflagellosus. 1310

Bacillus coagu/ans\ 179, 183, 1630 Bacillus coagulans Medium, 179 Bacillus oohnii, 75. 1304 Bacillus cyclohepianicus, 179, 195, 1% Bacillus cyclohepianicus Agar. 179 Bacillus cyclohepianicus Broth, 179 Bacillus faslidiosus. 84, 180. 1691, 1872. 1873, 1874 Bacillus faslidiosus Agar. 180 Bacillus faslidiosus Medium. 180 Bacillus filiformis, 180 Bacillus filiformis Medium., 180 Bacillus finnus, 1926 Bacillus gordonae. 1173 Bacillus haloalkaliphilus, 1311 Bacillus halodenitrificuns, 180. 1924 Bacillus halodenitrificans Agar, 180 Bacillus halophilus, 1038. 1153,1155, 1200 Bacillus infermus, 1241 Bacillus kaustophilus, 183 Bacillus laewlacticus, 183,745,768, 1273 Bacillus larvae. 100.254 Bacillus laterosporus, 1330 Bacillus ientimorbus. 100, 1842 BacilhtS lichenifornus\ 181,644, 1330, 1787, 1928 Bacillus macerans, 182 Bacillus macquariensLs, 1417 Bacillus macroides, 761. 952 Bacillus mascerans, 181 Bacillus mascerans Medium. 181 Bacillus Medium, 181 Bacillus megaterium. 176, 177.207,483. 592.713,1412, 1832 Bacillus methanolictLs. 232 Bacillus mycoides, 811, 1419 Bacillus naganoensis, 72. 73. 1829. 1833 Bacillus pacificus, 1841 Bacillus pallidas, 1332 Bacillus panlolhenticus, 1871 Bacillus pasteurii, 182 Bacillus pasteurii Agar, 182 Bacillus pasteurii Medium, 182 Bacillus pasteurii NH4 Ylv Medium. 182 Bacillus pasteurii Sanitation Agar, 182 Bacillus polytnyxa. 182.488.644. 1312 Bacillus polymyxa Agar, 182 Bacillus popUlae. 100. 182.183.888 Bacillus papillae Maintenance Medium, 182 Bacillus popillae Medium. 183 Bacillus pseudogordonae, 344 Bacillus Pullulan Salts. 183 Bacillus pumilus. 1305 Bacillus racemilacticus, 183. 184, 768 Bacillus racemilacticus Agar, 183 Bacillus schlegelii, 184, 185, 1042 Bacillus schlegelii Agar, 184 Bacillus schlegelii Broth, 18-1 Bacillus schlegelii Chemolithotrophic

Growth Medium, 185 Bacillus schlegelii 1 leterotrophic Growth Medium, 185

Bacillus schlegelii Medium. 184, 185 Bacillus selenitireducens, 186 Bacillus selenitireducens Medium. 185 Bacillus species, 53, 72, 73, 74, 75, 76, 83, 84,100. 176.177. 181. 183. 189. 241. 435, 444, 749,812, 814,832, 865, 868, 888.1005.1048.1053.1178.1261.1309. 1310, 1311,1312, 1316,1362,1365, 1416,1451,1538,1586,1587,1704, 1729. 1854. 1873. 1901.1928. 1930 Bacillus sphaericus, 206, 1281, 1418, 1445, 1598 Bacillus sporothermodurans, 215, 248 Bacillus stearolhermophilus. 53.63, 186. 187,1042,1330,1405,1569,1570,1611, 1823 Bacillus stearolhermophilus Broth. 186 Bacillus stearolhermophilus Defined Broth, 186 Bacillus stearolhermophilus Sporulation Broth. 187 Bacillus submarinus. 83 Bacillus subtilisM, 122.124.127,129,131, 134. 181, 428.450, 639.644. 751. 934, 936, 937, 997, 1168, 1312, 1315, 1361, 1412.1513.1592.1686.1853.1898 Bacillus subtilis NRR1. B-765, 1645 Bacillus subiilis wild typo strains, 1189 Bacillus sulfasportare. 1526 Bacillus thermoaciduranst 1712 Bacillus thermoalcalophilus, 187 Bacillus thernioalcalophilus Medium. 187 Bacillus ihermoantarcticus, 187 Bacillus ihermoantarcticus Medium, 187 Bacillus thermocloacae. 1332 Bacillus thermoglucosidasius. 182. 187 Bacillus thermoglucosidasius Agar, 187 Bacillus thermoleovorans, 187, 1185, 1186 Bacillus thermoleovorans Medium, 187 Bacillus thermoruber, 767 Bacillus thiaminolylicus. 1587 Bacillus thuringiensis, 121,188, 1303 Bacillus thuringiensis Medium, 187 Bacillus tusciae, 188,799 Bacillus tusciae Medium. 188 Bacillus xerothennodurans, 1852 Bacillus Xylose Salts. 188 Bacteremia, 908, 953 Bacterial Cell Agar. 189 Bacterial motility, 1225 Bacterial symbionts, 1345 Bacterionema helcogenes, 1890 Bacteriophage. 931. 938. 1189 Bacteriophage lysates, 931 Bacteriophage lysates production. 1470, 1471 Bacteriovorax litoralis, 1428 Bacterium anilratum, 1561 Bacterium DSM 4661, 509 Bacterium DSM 6754, 1384 Bacterium DSM 6780, 1050 Bacterium DSM 8385, 159

Index

Bade hum DSM 11262,1212 Bacterium DSM 12045, 887 Bacterium DSM 12047.1582 Bacterium DSM 12558, 1917 Bacterium DSM 12559, 1917 Bacterium DSM 12595, 1917 Bacterium DSM 13023. 1855 focterion DSM 13418,1689 Bacterium Medium. 189 Bacterium species, 189 Bacteroides asaccharolyticus, 191 Bacteroides Bile Esculin Agar. 189 Bacteroides cellulosolvens. 190. 200 Bacteroides cellulosolwns Medium, 190 Bacteroides disiens, 370 Bacteroides dislasonis. 225, 366, 703 Bacteroides eggerthii, 366, 1459 Bacteroides /orsythus. 1274 Bacteroides fragilis, 219, 220, 366, 702, 1772 Bacteroides Bacteroides Bacteroides Bacteroides Bacteroides

galacturonicus, 105, 1918 gingivaiis. 370 gracilis, 372, 701 helcogenes, 366.1459 I li Veg Agar Base with Selective

Supplement, 190 Bacteroides macacae. 366. 370 Bacteroides Medium, 190 Bacteroides melaninogenicus, 191. 332, 892 Bacteroides nodasus, 191, 1890 Bacteroides nodosiis Agar, 191 Bacteroides ovalus, 225. 703 Bacteroides pectinophilus. 105 Bacteroides pneumosinles, 659 Bacteroides praeacutus. 1461 Bacteroides pyogenes, 366, 1459, 1890 Bacteroides ruminicola. 616, 1890 Bacteroides species, 101, 106,189,190,219, 220. 269. 275. 333, 369. 371.617,718. 860. 939, 963. 1419, 1459, 1485,1549, 1890 Bacteroides splanehnicus. 366 Bacteroides succinogenes, 616 Bacteroides suis, 366. 1459. 1890 Bacteroides thetaiotaomicron, 225, 332, 702 Bacteroides uniformis. 225. 703, 1459 Bacteroides ureotyticus, 281, 370, 372, 701 Bacteroides vutgatus. 191. 225. 703 Bacteroides vidgatus Medium, 191 Bacteroides xylanolylicus. 1459 Baeomyces roseus. 951 BAFAgar. 191 BAGG Broth, 191 BAGG Broth Base with Glycerol. 191 BAGG Hi Veg Broth Base with Glycerol. 192 Baird Parker Agar Base with Egg Yolk Tellurite Enrichment, 192 Baird Parker Agar Base, HiVeg with Egg Yolk Tellurite Enrichment. 193 Bail d-Parker Agar, 192,194 Baird-Parker Agar, Supplemented. 193 Baird-Parker Egg Yolk Agar (ISO), 193

Balamuth Medium, 194 Balamuthia mandrillaris, 1519 Balneatrix alpica, 440 Bandoni's MYP Medium, 196 Barbour Stoenner Kelly Medium. 276 Bartonella bacillifonnis, 1833 Bartonella elizabelhae. 813 Bartonella henselae, 813 Bartonella quintana. 813,1476 Basal Medium,, 196 Basal Mineral Medium, 197 Basal Synthetic Medium. 197 Basal Thcrmophilc Medium, 197 Base Agar, 122, 131 Base Agar with low pll, 122. 132 Base Cholesterol Medium. 198 Base 1 li Veg Agar w/ low pi 1), 127 Base 1 .aver Agar with Nutrient Overlay Agar, 198 Basic Cultivation Medium. 198 Basic Mineral Medium, 199 Basidiobolus micrasporus. 767 Basidiobolus ranaruiiK 1003 Basidiomycetes species, 998 Basipetospora halophila, 1555, 1557 Bauer-Kirby method. 1250 BBE. 190 BBi: Agar, 189 BBGS Agar. 221 BC Medium, 199 BC Motility Medium. 200 BC Motility Test HiVeg Medium, 200 BCA. 189 BCG Glucose Agar. 200 BCG Glucose HiVeg Agar. 200 BCG vaccine, 753,1540 BCG-Glucosc Agar. HiVeg. 1583 BCM, 178 BCM Bacillus cereus Group Plating Medium, 218 BCM for Usteria monocytogenes, 222 BCM 0157:117(+) Plating Medium, 200 BCP Azidc Broth. 200 BCP Broth, 264 BCP D Agar, 201 BCP DCLS Agar, 201 BCP MS G Agar, 265 BCYE a Agar. Modified, 942 BCYE Agar, 201 BCYE alpha Base. 201 BCYE Differential Agar, 201 BCYE Medium. Diphasic Blood Culture. 202 BCYE Selective Agar with CCVC, 202 BCYE Selective Agar with GPVA. 203 BCYE Selective Agar with GVPC. 203 BCYE Selective Agar with PAC, 204 BCYE Selective Agar with PAV. 204 BCYEct with Alb, 204 BCYEa without [/Cysteine, 205

BCYT, 1125 Bdellovibrio bacteriovorus. 205,604. 1367, 1690

1965

Bdellovibrio Medium. 205 Bdellovibrio species. 1280, 1820. 1926. 1951, 1952 Bdellovibrio starrii, 205 Bdellovibrio stolpii. 1367 Bean Agar, 206

Beef extinct, 3 Beef Extract Agar, 206 Beef Extract Agar, Hi Veg. 206 Beef Extract Broth, 206 Beef Extract Broth. HiVeg. 206 Beef Extract Peptone Serum Medium, 206 BeefExtractV.207 Beef Extract with Sodium Chloride. 207

Beef Heart, 3 Beef Infusion Agar. 207 Beef Infusion Broth, 207 Beef Liver Medium for Anaerobes, 207 Beer, 1478,1867 lkcr processing. 1911 Beggialoa Agar, 207 Beggiatoa alba, 207. 208 Beggialoa Broth, 208 Beggiatoa Medium, 208 Beggiatoa species, 197.208.665.1227.1256 Beggiotoa and Thiothrix Medium. 207 Beijerinck's Thiobacillus Medium. 209 Beijerinckia acida, 1296 Beijerinckia Agar. 208 Beijerinckia derxii, 167, 168, 169, 209 Beijerinckia jluminensis. 209, 1296 Beijerinckia indica, 209, 1296. 1297 Beijerinckia Medium, 209 Beijerinckia Medium, Modified, 209 Beijerinckia mobilis. 209, 1296 Beijerinckia species, 170, 209, 827, 828 Bellilinea caldistulae. 113 Beimel's HiVeg Agar. 210 Bennett's Agar. 209 Bennett's Agar wiUi Maltose, 210 Bennett's Agar with Sucrose, 210 Bennett's Medium. 210 Bennett's Modified Agar Medium, 210 Benzene Sulfonate Medium, 210 ik'ti/oalc Medium, 211 Bcnzoatc Medium II, 211 iknzoatc Minimal Salts Medium, 211 Bcnzoatc Nitrate Salts Medium. 211 Iknzylcyanide utilizing bacteria, 1172 Bergeyella zoohelcum, 1830 (5-u-galactopyranosidase production, 386 P-galactosidasc. 1327 [^-hemolytic streptococci, 1585 Betabacterium Medium. 212 Betabacterium species, 212 Bettsia alvei, 1004 Beutenbergia cavernae. 1513 Ikverages, 761 BG 11 Agar. 212 BG 11 Marine Agar, 213 BG 11 Marine Broth, 213 BG 11 Medium, 213

1966

Index

BG11 Uracil Agar, 214 BG 11 Uracil Broth, 214 BO Sulfa Agar, 212 IKi Sulfa IliVeg Agar, 212 Bill, 244 BHI Agar, 244 Bill Agar 0.7%. 232. 245 BUI Broth, 232, 249 Bill Glucose Medium, 214 Bill Medium, 215 Bill with Krylhromycin. 251 Bill with Glucose, 214 Bill with Glycerol and Reducing Agents, 215 BIII with Serum and Glucose, 253 BHl/1 Medium. 215 BH1/2 Medium, 215 BHI/3 Medium. 215 BI11S. 253 BIIIV Agar, 1/10,248 BIIIY Media. 216 Bicarbonate Agar, 216 Bicosoeca vacillans. 1015 Bifidobacterium adolescentis, 217,1890 bifidobacterium Agar, 216 Bifidobacterium angulation, 217 Bifidobacterium animalis. 217 Bifidobacterium asteroides, 217, 1125 Bifidobacterium hifidum, 217. 921. 1890 Bifidobacterium bourn, 217 Bifidobacterium breve. 217. 1890 Bifidobacterium Broth. 216 Bifidobacterium calenulatum, 217 Bifidobacterium choerinum, 217 Bifidobacterium coryneforme, 217, 920 Bifidobacterium cunicuti. 217 Bifidobacterium den Hum, 217 Bifidobacterium gallicum, 217 Bifidobacterium indicum, 217 Bifidobacterium infantis, 216. 217 Bifidobacterium longum, 217,1890 Bifidobacterium magnum, 217 Bifidobacterium Medium, 216. 217 Bifidobacterium merycicum, 217. 1486 Bifidobacterium minimum. 217 Bifidobacterium pxeudocatenulatum, 217 Bifidobacterium [tseudolongum. 217 Bifidobacterium pullorum, 217 Bifidobacterium ruminanlium. 217 Bifidobacterium saeculare, 217 Bifidobacterium species. 216.217,225. 369. 663, 664, 718, 1486, 1776, 1779, 1801, 1805.1824 Bifidobacterium subtile, 217 Bifidobacterium suis, 217 Bifidobacterium thermophilum, 217 BiGGY Agar, 217 Bile Broth Base with Streptokinase. 218 Bile Broth Base, IliVeg with Streptokinase, 217 Bile Ksculin Agar, 217, 218 Bile Ksculin Agar with Kanamyctn. 219 Bile Fsculin Agar, IliVeg, 218

Bile Ksculin Azide Agar, 219 Bile Ksculin A/ide IliVeg Agar, 219 Bile lisculin IliVeg Agar Base with Ksculin. 219 Bile Ksculin IliVeg Agar with Kanamycin, 220 Bile Oxalate Sorbose Broth. 220 Bile Peptone Transport Medium, 221 Bile Salt Agar with Streptokinase. 221 Bile Salts Brilliant Green Starch Agar. 221 Bile Salts Gelatin Agar, 221 Bile tolerance, 218 Bile-tolerant bacteria. 1773 BIN Medium, 221 Diolumincsccnt bacteria, 1401 Biosynth Chromogcnic Medium for Listeria monocytogenes, 222 Biotin Assay Medium. 222 Biphasic Medium for Neisseria. 222 Biphenyl Agar. 222 Biphenyl utilization, 1010 Biphenyl utilizing bacteria. 223. 1043. 1404 Bipolaris leersiae. 1877 Bipolaris micropus, 1877 Bipolaris sacchari, 614 Bipolaris sorghicola, 588. 615 Bird Seed Agar, 223 Bismuth Sulfite Agar. 223. 224 Bismuth Sulfite Agar, I liVeg. 223 Bismuth Sulfite Agar. Modified. 224 Bismuth Sulfite Agar, Modified, IliVeg, 224 Bismuth Sulfite Broth. 224 Bismuth Sulfite Glucose Glycerin Yeast Extract Agar, 217 Bivalves. 1535 BL Agar, 225 Blaser's Agar, 307 Blascr's Campylobacter Agar. 298 Blaser-Wang Campylobacter Medium, 307 Blaslofxicfer aggregatus, 1461, 1462 Blastnhacter cap<:ulatus, 1461, 1462 Blastobacter denitrificans. 225. 226. 1461, 1462 Blastobacter denitrificans Agar, 225 Blastolxicter Knrichmcnt Medium. 226 Blastol>ucler Medium, 226 Blastobacter natatorius, 226, 1455 Blastolxictcr species. 226 Blaslococcus aggregatus, 226 Blastococcus aggregatus Medium. 226 Blaslococcus saxobiidens, 971 Blaslocrilhidia culicis, 600. 759 Blastocrithidia leptocoridis, 249 Blastocysts Kgg Medium. 226 Blastocystis hominis. 226 Blastocysts species, 226 Blastomyces dermatiditis, 244 Blastomyces dermatifidis. 251, 849.850, 1399,1528,1586,1928

BI.K Hi Veg Broth Base with Listeria Selective Supplement, 224 Blood. 228. 270. 346. 347.440. 585, 587. 698, 942, 1159, 1166, 1528,1632, 1880, 1881 Blood Agar, 227 Blood Agar Base. 227. 228 Blood Agar Base No. 2,229 Blood Agar Base No. 2 with 1.2% Agar, HiVcg 1M ,229 Blood Agar Base No. 2,1 li Veg with Blood, 229 Blood Agar Base with 2.5% Sodium Chloride. 229 Blood Agar Base with 3.5% Sodium Chloride. 229 Blood Agar Base with Blood. 228 Blood Agar Base with Low pi I, IliVeg with Blood. 228 Blood Agar Base with Peptone, 228 Blood Agar Base with Special Peptone. 229 Blood Agar Base, IliVeg with Blood, 228 Blood Agar Base. Sheep. 228 Blood Agar No. 2, 230 Blood Agar with Low pi I, 230 Blood Agar. Diphasic, 230 Blood Agar, Diphasic Base Medium, 230 Blood Base Agar, 230 Blood Base Agar with Charcoal, 231 Blood Base Agar with Horse Blood. 231 Blood Base Agar with Horse Blood, Fumarateand Formate. 231 Blood culture, 244 Blood Free Campylobacter Selectivity HiVeg Agar Base, 231 Blood Glucose Cystine Agar. 231 Blood specimens, 953 Blood-free, 817 Blood-free Selective Medium, 331 Blue-Green Agar, 233 Blue-Green Broth. 233 Blue-Green Nitrogen-Fixing Agar, 233 Blue-Green Nitrogen-Fixing Broth, 234 BM Medium.. 232 BMM Agar. 234 BMM Broth. 234 BMPA-a Medium. 234 BMS Agar, 235 BNS.21I Bodo curvifilus, 1556 Bodo designis. 1015 Bodo saliens, 1556 Bodo species, 1589 Bodo variabilis, 1015 Body lluids. 1258.1776. 1779 Bogoriella caseUytica, 235 Bogoriella Medium, 235 Boletinellus meruiioides, 1065 Boletus edulis, 1002 Boletus granulatus, 1325 Boletus leucophaeus, 1065 Boletus luteus. 1325

Index

Boletus nibinellus. 775 Bolton Broth. 235 Bonner-Addicott Medium, 236 Bordet (iengou Agar, 236, 238 Bordetella bronchiseplica, 1283 Bonlelella parapertussis, 236,237,238,817, 1485, I486 Bo>detella pertussis, 236.237,238,345,346, 347.889, 1485. 1486 Bordetella pertussis Selective Medium with Bordet-Gengou Agar Base, 236 Bordetella pertussis Selective Medium with Charcoal Agar Base. 236 Bordetella Selective Supplement. 6 Bordetella species, 1830 Bordet-Gengou Agar Base with 1.6% Agar, 237 Bordet-Gengou Agar Base with Rabbit Blood and Glycerol, 237 Bordet-Gengou HiVeg Agar Base with 1.6% Agar. 237 Bordet-Gengou I iiVeg Agar Base with Rabbit Blood and Glycerol. 237 Bordet-Gengou Medium. 238 Borrelia afzelii, 278 Borreiia anserina. 278 Borrelia burgdorferi. 278. 894.895 Borrelia gorinii. 277 Borrelia hermsii. 239 Boireliu japonica, 278 Borrelia Medium. 238 Boirelia parkeri. 239 Borrelia species, 277 Bonelia turicatae, 239 Borrellia afzelii. 277 Botrellia burgdorferi. 277 BOS Brotli. 220 Bosea Medium, 232 Bosea species, 232 Bosea tluooxidans, 239 Bosea thiooxidans Medium, 239 Botrxdium becheriamnn, 70 Batrydium cystosum, 70 Batrydium stoloniferum, 70 Batryosphaeria berengeriana, 972 Botryolinia draylonii, 809 Botryotinia fuckeliana, 809 Botryolinia narcissicola, 809 Botryotinia polyblastis. 809 Botryotinia porri. 809 Botiyozyvia nematodophila. 1942 Botrytis aclada. 809 Botrytis alii, 239 Botrytis cinerea. 239, 809 Botrytis hyacinthi. 809 Botrytis Separation Agar, 239 Botrytis species, 239 Botulism, 398 Bouillon Medium, 239 Bovine albumin. 4

Bovine Albumin 1 ween 80 Medium. hllinghausen and McCullough, Modified, 240 Bovine Albumin 1 ween 80 Semisolid Medium, Kllinghausen and McCullough. Modified, 240 Bovine blood, citratcd, 4 Bovine blood, delibrinated, 4 Bovine mastitis. 630. 656 Bovine Serum Albumin Iween 80 Agar, 239 Bovine Serum Albumin I ween 80 BroUi, 239 Bovine Serum Albumin Iween 80 Soft Agar. 240 BPHD Medium, 241 I JIM. Agar. 241 BPL HiVeg Agar. 242 BPSA, 285 Brachiomonas submarina, 1808 Brachyhacterium faecium, 1428 Brachybacterium species, 1830, 1836 Brachyspira innocens. 1831 Brackish Acetate, 242 Brackish Prosthecomicrobium Medium, 243 Brackish specimens, 1041 Brackish Water Ameha Medium, 243 Bracteacoccus grandts. 70 Braiiyrhizobiuinjaponicum. 1490, 1491. 1936 Bradyrhizobium species, 1008 Brain I leart OC Agar. 244 Brain Ik-art CC Agar, HiVeg, 244 Brain Heart Cycloheximide Chloramphenicol Agar. 244 Brain Heart Cycloheximide Chloramphenicol Agar, I Ii Veg, 244 Brain Heart Infusion. 244 Brain He-art Infusion Agar. 232.244, 245. 249 Brain Heait Infusion Agar 0.7%, 232, 245 Brain Heart Infusion Agar with 1% Agar, Hi Veg. 245 Brain Heart Infusion Agar with 1% Agar. HiVeg with Penicillin. 246 Brain Heart Infusion Agar with 10% Sheep Blood. Gcntamicin and Chloramphenicol, 247 Brain Heart Infusion Agai with Chloramphenicol. 246 Brain I leart Infusion Agar with Cysteine, 246 Brain Heart Infusion Agar widi Kanamycin, 246 Brain I leart Infusion Agar with Penicillin and Streptomycin. 247 Brain Heart Infusion Agar with Twccn1M 80, 247 Brain Heart Infusion Agar with Vitamin B ^ . 247 Brain Heart Infusion Agar, 1/10 with Vitamins, 248 Brain Heart Infusion Blood Agar, 249 Brain Heart Infusion BroUi, 232, 249,250 Brain Heart Infusion Brotli with 6.5% NaCl. HiVeg,250 Brain Heart Infusion Broth, Hi Veg, 250

1967

Brain I leart Infusion Casein Starch, 250 Brain Heart Infusion Soil I'xlrac! Medium, 254 Brain I leart Infusion with 0.1 % Agar, I liVeg, 250 Brain Heart Infusion with 0.7% Agar, 245 Brain Heart Infusion with 3% Sodium Chloride, 253 Brain Heart Infusion with 5% Sodium Chloride. 253 Brain Heart Infusion with Agar. Yeast Extract. NaCl, Inactivated Horse Serum. and Penicillin, 248 Brain Heart Infusion with Agar. Yeast Extract, Sucrose, Horse Serum and Penicillin, 248 Brain Heart Infusion with Agar, Yeast Extract, Sucrose, Inactivated I lorse Serum and Penicillin. 248 Brain Heart Infusion with Casein, 250 Brain Heart Infusion with Chicken Serum. 250 Brain Heart Infusion with Cystine. 251 Brain Heart Infusion widi Erythromycin, 251 Brain I leart Infusion with Glucose and I lorse Serum. 251 Brain Heart Infusion with Horse Blood. 251. 1833 Brain Heart Infusion with PABA. 252 Brain I leart Infusion widi PABA and Agar. 252 Brain Heart Infusion with PABA and Agar. HiVeg, 252 Brain Heart Infusion with p-Aminobcnzoic acid, 252 Brain Heart Infusion wiih/>-Aminobcnzoic acid and Agar. 252 Brain I leart Infusion with Para-aminobenzoic Acid, HiVeg, 252 Brain Heart Infusion with Ralibit Serum, 252 Brain Heart Infusion with Rabbit Scrum and Yeast IJctract, 253 Brain Heart Infusion with Serum and Glucose, 253 Brain I leart Infusion with Sucrose and Horse Serum, 253 Brain Heart Infusion with Thiamine. 254 Brain Heart Infusion, Supplemented, 253 Brain Liver Heart Semisolid Medium, 254 Branhamella catarrhalis, 215, 721, 722 Bretlanomyces Agar Base with Selective Supplement, 254 Bretlanomyces anomalus, 999 Bretlanomyces bruxel/ensis. 999 Bretlanomyces claussenii. 999 Bretlanomyces lambicus, 999 Bretlanomyces species, 255 Brevibacillus invocatus. 1316 Brevibacillus levickii Medium,. 255 Brevibacillusspecies, 1630, 1830 Bwibacillus laterosporus, 1316 Brevibacterium acetylicum. 1470

1W.X

Index

Brevibacterium alkanophilunu 255 Brevibacterium casei. 454, 1403 Brevibacterium epedermidis, 1403 Brevibacleriunt epidermidis, 454 Brevibuclerium helvolum, 453 Brevibacleriunt inceitum, 454,455 Brevibacterium iodinuin, 454 Brevibacterium lactoj'ermentum, 1452 Brevibacterium linens, 348,453,454, 1470, 1842 Brevibacterium liquefaciens, 454 Brevibacterium Medium, 255 Brevibacterium oxydans, 454 Brevibacterium species. 189.255.454.1365, 1932 Brevibacleriunt stationis, 454, 1554 Brevigemma cellulytica, 19 Brevundimonas Agar, 255 Brevundimonas diminuta, 1306 Brevundimonas species, 255 Brewer Anaerobie Agar, 255 Brewer lhioglycollatc HiVeg Medium. 256 Brewer lhioglycollatc HiVeg Medium. Modified. 256 Brewer lhioglycollatc Medium. 256 Brewer lhioglycollatc Medium, Modified, 256 Breweries, 942, 1912 Brewery isolates, 1652, 1653 Brewing, 977, 1552, 1912 Brigg's I.iver Brolh pH 7.0, 257 Brigg's fiver Tomato Broth, 257 BRII.A.261 BRII.A MUG Broth. 257 Brillant Green. 2%-Bilc MUG Broth, 257 Brilliance™ Bacillus ceretts Agar, 257 Brilliance1" Candida Agar. 257 Brilliance'" E. co/i/Coliform Agar, 258 Brilliance1" E. co/i/Coliform Selective Agar. 258 Brilliance™ Enterobacter sakazakii Agar, 258 Brilliance™ KSBL Agar. 258 Brilliance™ Listeria Agar. 258 Brilliance™ MRSA Agar, 259 Brilliance™ Salmonella Agar. 259 Brilliance™ UT1 Agar. 259 Brilliance™ 1711 Clarity Agar, 260 Brilliant Green, 2%-Bile Broth, Hluoroculf*. 261 Brilliant Green Agar, 260 Brilliant Green Agar Base with Phosphates and Sulfa Supplement, 260 Brilliant Green Agar with Sulfadiazine. 261 Brilliant Green Agar, Modified. 260 Brilliant Green Bile Agar. 261 Brilliant Green Bile Broth. 261 Brilliant Green Bile Broth with MUG, 261 Brilliant Green Broth, 262 Brilliant Green HiVeg Agar, 262

Brilliant Green HiVeg Agar Base Modified with Sulfa Supplement, 262 Brilliant Green HiVeg Agar Base with Sulfa Supplement, 262 Brilliant Green HiVeg Broth. 2%. 263 Brilliant Green Lactose Bile Broth, 261, 263 Brilliant Green Phenol Red Agar, 241, 263 Brilliant Green Phenol Red HiVeg Agar, 242 Brilliant Green Sulfa HiVeg Agar. 212 Brilliant Green Sulfapyridine Agar, 212 Brined vegetables. 960. 1886 Brochothrix campeslris. 1428 Brochothrix thermosphacta, 263,453,454, 746.1627,1650. 1830 Brochothrix thermosphacta Medium, 263 Brodie Medium. 263 BROLACTN MUG Agar, 263 Bromcresol lHirplc Azide Broth. 200 Bromcresol Puq>le Broth, 264 Bromcresol Purple Broth with Sodium Chloride, 264 Bromcresol l*iirple Deoxycholate Agar, 201 Bromcresol Purple Deoxycholate Citrate Lactose Sucrose Agar, 201 Bromcresol l\irple IX*xtrose Broth, 264 Bromcresol Purple HiVeg Broth Base, 1932 Bromcresol l*urple Milk Yeast hxtract with CCG, 265 Bromo Cresol Purple Azide HiVeg Broth. 265 Bromo Crcsol Purple I li Vcg Broth Base. 266 Bromocresol Purple Milk Solids Glucose Agar, 265 Biomothymol Blue Lactose Cystine MUG Agar. 263 Bromthymol Blue Agar. 266 Bromthymol Blue Brolh. 266 Bromthymol Blue Lactose Agar, 280 Bromthymol Blue Lactose HiVeg Agar, 280 Brooks Agar, 267 Broth dilution susceptibility testing method, 858 Brown and Scott Modified, 1168 Bnicella abortus, 1416 Brucella Agar, 267 Brucella Agar Bax-Campylobacter Medium, 267 Brucella Agar with 1.0% Glucose, 267 Brucella Albimi Broth. 270 Brucella Albimi Broth with 0.16% Agar. 268 Brucella Albinii Broth with 0.16% Agar and, 1% Glycine. 268 Bnicella Albinii Broth with Agar and, 1.5% Sodium Chloride. 268 Bnicella Albimi Broth with formate and Humarate, 268 Bnicella Albinii Broth with Sheep Blood. 268 Brucella Albimi Medium, Semisolid, 269 Bnicella Anaerobic Blood Agar. 269 Brucella Blood Agar with Hemin and Vitamin K,, 269 Bnicella Blood Culture Broth, 269

Brucella Broth. 270 Brucella Broth Base Campylobacter Medium, 271 Brucella Broth with 0.16% Agar. 270 Brucella Broth with Additives. 270 Brucella Broth. Modified. 271 Brucella IBP Agar, 271 Brucella I BP Broth, 271 Brucella I li Veg Agar Base with Blood and Selective Supplement. 272 Brucella HiVeg Agar Base, Modified with Blood and Selective Supplement, 272 Brucella HiVeg Broth Base with Blood and Selective Supplement. 272 Brucella Medium Base, 273 Brucella Selective Medium. 273 Brucella Selective Medium with Blood and Semm. 273 Brucella Selective Supplement, 6 Brucella Semisolid Medium with Cysteine, 273 Brucella Semisolid Medium with Glycine. 273 Brucella Semisolid Medium with Nitrate. 274 Brucella Semisolid Medium with Sodium Chloride. 274 Brucella species, 267, 268, 270, 271, 272, 273.960. 1565. 1566. 1847. 1848.1850 Bryant and Burkey Agar, 274 Bryant and Burkey Medium, 274 Bryant-Robinson Medium, 274 BS Medium, 275 BSA Twecn 80 Agar. 239 BSA Twecn 80 Broth. 239 BSA Tween 80 Soft Agar, 240 BSK. Medium, 276 BSK Medium. Modified, 277 BSK Medium, Revised, 277 BSL for Corynebacterium, 278 BSR Medium, 278 BSTSY Agar, 279 BT Medium, 279 BIB Lactose Agar, 280 BIB Tccpol* Agar. 280 BTU Medium. 280 Buellia stillingiana, 951 Buffered Azide Glucose Glycerol Brolh, 191 Buffered Azide Glucose Glycerol Broth Base, 191 Buffered Azide Glucose Glycerol HiVeg Broth Base. 192 Buffeted Charcoal Yeast Hxtract Agar, 201 Buffered Charcoal Yeast Hxtract Agar with Albumin, 204 Buffered Charcoal Yeasl Extract Agar without t.-Cysteine, 205 Buffered Charcoal Yeast Hxtract Differential Agar, 201,281 Buffered Charcoal Yeast Hxtract Medium. Diphasic Blood Culture, 202

Index

Buffered Charcoal Yeast Extract Selective Agar with Cephalothin, Colistin, Vancomycin and Cyclohcximidc, 202 Buffered Charcoal Yeast Extract Selective Agar wiili Glycine, Polymyxin B, Vancomycin, and Anisomycin, 203 Buffered Charcoal Yeast Extract Selective Agar with Glycine. Vancomycin, Polymyxin B. and Cycloheximide. 203 Buffered Charcoal Yeast Extract Selective Agar with Polymyxin B, Anisomicin and Vancomycin. 204 Buffered Charcoal Yeast Extract Selective Agar with Polymyxin B, Anisomycin and Celamandole, 204 Buffered Clostridial Medium with Cellobiose,281 Buffered Enrichment Broth, 282 Buffered Glucose HiVeg Broth, 282 Buffered HiVeg Peptone Water, 282 Buffered IliVeg Peptone Water with Sodium Chloride. 282 Buffered listeria Enrichment Broth Base wilh Listeria Selective Supplement, 282 Buffered Listeria Enrichment HiVeg Broth Base with Listeria Selective Supplement, 226 Buffered Marine Yeast Medium, 283 Buffered Peptone Water, 283 Buffered S & II Agar, 283 Buffered S & II Broth. 284 Buffered Soy Lactose for Corynebacterium, 278 Buffered I ryptone Glucose Yeast Extract Broth, 284 Buffered Yeast Agar, 284

Buffered Yeast Extract Broth, 288 Bullera crocea. 1942 Bumilleria sicula, 70 Burke's Medium, 285 Burke's Modified Nitrogen-Free Medium, 284 Burke's Modified Nitrogen-Free Medium with Benxoatc. 285 Burkholileria cepacia, 285, 1045 Burkholderia cepacia Agar. 285 Burkholileria glttmae. 1420 Burkholderia phenazinium. 1484 Burkholderia pickettii. 1446 Burkholderia plantarii, 1420 Burkholderia pseudomallei. 285 Burkholderia pseudomallei Selective Agar, 285 Burkholderia species, 1045, 1304, 1484, 1830 Burkholderia vietnamietisis, 1830 Bushncll-Haas Agar. 286 Bushnell-Haas Broth, 286 Bushncll-Haas Medium, 286 Butanediol Medium, 286 Butter, 1814

Butyribacleriiim methylofrophicum, 234, 1076 Butyrivibrio crossotus. 367.1419 Butyrivibrio fibrisolvens, 616, 617, 1369, 1419.1522 Butyrivibrio species, 618 Butyrivibrio Species Medium, 286 Butzlcr's Campylobacter Medium, 307 BY Agar Medium, 287 By- Medium. 287 BYE Agar, 287 BYE HiVeg Agar with Blood. 288 BYE HiVeg Broth with Blood, 288 BYEB. 288

C, 3G Spiroplasma Medium, 288 C, 3N Spiroplasma Medium. 289 C.E.E.I). HiVeg Agar Base without Indicator. 393 C.E.E.D. I IiVeg Agar with Andrade's Indicator. 392 CJLRD. HiVeg Agar with Bromthymol Blue, 392 C.L.E.D. MUG Agar. 263 C/10 Agar, 288 C/10 Medium Rcichenbach. 288 CA YE Broth, 321 Cadmium Fluoride Acriflavin Tellurite Medium. 344 CAE Agar Base with Triphenyltelra/olium Chloride, 290 CAE HiVeg Agar Base with Triphenyltetra/olium Chloride. 290 ('aecitellus parxtilus. 1015 CAFC Test Medium. 291 Cafeteria minuta, 1556 Cafeteria roenhergensis, 1556 Caffcic Acid Agar. 291 Cafl'eic Acid Ferric Citrate Test Medium, 291 Caffeine Medium. 291 CAGV Medium. 319 CAE Agar. 291 CAE Broth, 291 CAE HiVeg Agar, 292 CAE HiVeg Broth, 292 Calcarisporium arhuscula, 348 Calcium Caseinate Agar. 292 Calcium Caseinate Agar with Skim Milk, 292 Calderobacterium hydrogenophilum, 862 ('aldicelluhsiruplor kristjanssouii, 174 CaldiceUuhsiruptor lactoaceticus, 173. 174 CaldiceUulosiruptor Medium, 292, 293 CaldiceUuhsiruptor saccharolylicus, 293 Caldisphaera Medium,, 293 Caldisphaera species, 294 Calditerrivibrio Medium. 294 Calditerrivibrio species, 294 Caldithrix abyssi, 1279 Caldithrix abyssi DSM 13497, 1280 Caldivirga maquilingensis, 295 Caldivirga Medium, 294

1969

Caloplaca aurantuica, 951 Caloramator proteoclasticus, 296 Caloramator proteoclasticus Medium, 295 Caloramator viterbiensis. 296 Caloramator viterbensis Medium, 296 Caloranaerobacter azorensis. 1023 Calothrix species. 213. 214. 233, 234 Calymmatohacter granulomatis, 613 Calymmatobacterium granulomalis, 296 Calymmalohacterium granulomalis Semidefincd Medium, 296 CAMG Broth, 2 % Caminicella Medium, 297 Caminicella sporogenes, 297 CAMP test. 1826. 1827, 1835 Camphor Minimal Medium. 297 Camphor utilization, 1173 ( amposporium pelluculum, 1877 Campy BAP Medium. 307 Campy Ccfcx Agar. 305 Campy TWO Medium. 297 Campylo I hioglycollale HiVeg Medium, Base with Selective Supplement. 297 Campylobacter Agar. 297. 298 Campylobacter Agar with 5 Antimicrobics and. 10% Sheep Blood. 298 Campylobacter Agar. Blascr's. 298 Campylobacter Agar, Skirrow's, 299 ('ampylobacter Blood Free Preson Agar with Celbpera/one, Amphotericin, and Teicoplanin, 328 Campylobacter Blood-Frcc Agar Base. Modified, 299 Campylobacter Blood-Free Selective Agar. 299 Campvhbacler Charcoal Differential Agar. 299 Campylobacter coli, 270, 271, 299, 1796, 1819. 1890 Campylobacter concisus, 1890 Campylobacter curvus, 306 Campylobacter divergens, 225, 718 Campylobacter Enrichment Broth, 300. 301. 302 Campylobacter Enrichment HiVeg Broth Base with Blood and Antibiotics, 303 Campylobacterfecal is, 270, 271, 303 Campylobacterfecalis Medium, 303 CampyloJ>acterfetus, 271, 303. 304. 693, 694, 1890 Campylobacterfetus Medium. 303. 304 Campylobacter fetus Selective Medium, 304 Campylobacter gracilis. 1304 Campvhbacler growth supplement, 4 Campylobacter I IiVeg Agar Base with Blood ami Antibiotic Supplement. 304 ('ampylobacter I IiVeg Agar Base with Blood and Selective Supplement. 304 Campylobacter hyointestinalis, 1890 Campvhbacler Isolation Agar A, 305 ('ampylobacter Isolation Agar B, 305

1970

Index

Campylobacter jejuni, 231, 267, 271, 298. 299, 309, 331, 693,694, 1796, 1819, 1890 ('ampylohacler lari, 1890 Campylobacter laridis. 299 Campylobacter Medium, 305 Campylobacter mucosalis, 268. 306, 372, 1890 Campylobacter mucosalis Medium, 306 ('ampylohacler Nitrate Broth, 306 Campylobacter Nitoalc IliVeg Broth. 306 Campylobacter nitrojigilis, 268 Campylobacter pylori. 721 Campylobacter rectus, 306, 1304 Campylobacter rectus Medium. 306 ('ampylohacler Selective Medium, BlaserWang. 307 Campylobacter Selective Medium, Butzlers, 307 ('ampylohacler Selective Medium, KarmaIt's, 308 ('ampylohacler Selective Medium, Preston's, 308 ('ampylohacler Selective Supplement Blaser-Wang, 6 Campylobacter Selective Supplement But/ler, 6 Campylobacter Selective Supplement Preston, 6 Campylobacter Selective SupplenKnt Skirrow, 6 Campylobacter species, 17,231, 268, 269, 273. 274. 297, 298.299, 300.301, 302, 303, 304, 305, 306,307, 308,309, 329, 331,461.468. 606.607, 701,706, 766, 1202,1249, 1290,1345,1346, 1427, 1562, 1563, 1576, 1796, 1819, 1901, 1902.1903 Campylobacter sputorum, 215, 309, 1890 Campylobacter sputorum subspecies bubulus, 309 Campylobacter sputorum subspecies bubulus Medium, 308 Campylobacter sputorum subspecies mucosalis. 309 Campylobacter sputorum subspecies mucosalis Medium. 309 Campylobacter'\'\\\og\yco]]iilc Medium with 5 Antimicrobics. 309 Candelariella vitellina, 951 Candida Agar. 309 Candida albicans, 17,217, 258, 309, 310, 311,351.376.450.451.480,843.1222. 1339,1513, 1514, 1799, 1909, 1913, 1941.1949 Candida albicans NRRI. Y-477, 1645 Candida BCG Agar Base. 309 Candida BCG IliVeg Agar Base with Neomycin, 310 Candida boulinii, 589, 1949 Candida Bromcrcsol Green Agar Base. 309 Candida Diagnostic Agar, 310

('andida dubliniensis. 258. 310 Candida ethanolica, 589 Candida famata, 1418 Candida glahrata, 310, 311, 1000 Candida glaebosa. 1476 Candida haemulonii, 1000 Candida IliVeg Medium with Antibiotics. 310 Candida Isolation Agar. 310 Candida Av/jr, 310, 1909 Candida kefyr ATCC 28838.1937 Candida knisei. 217, 310, 311. 1222 Candida lactiscondensi, 1000 ('andida lipolytica, 1949 Candida hisitaniae, 258 Candida magnoliae. 1000 Candida maltosa, 589 Candida Medium with Antibiotics, 311 Candida melihiosica, 1001 Candida nemodendra. 1000 Candida parakrusei, 217 Candida parapsilosis. 310 Candida pinlolopesii, 318, 1949 Candida pseudotropicalis. 217, 310 C 'andida species, 17,217,310,311,351,376, 384.691,749.832.833.998.1222.1339, 1513,1514,1942, 1949 Candida steilatoidea, 217,310. 1222. 1513. 1514 Candida Iropicalis, 17, 217, 310, 311,376, 589, 1222.1909, 1941 Candida utilis, 134, 141 Candida versatilis. 1001. 1803 CandiSelect4â&#x201E;˘,311 Canned foods. 46. 47. 461. 1309,1316 Cantharellus Agar, 311 Cantharellus cibarius. 311 Capniomyces sleilatus. 477, 1146, 1839 Capnocytophaga gingivalis, 1576, 1890 Capnocytophaga granulosa. 312 Capnocytophaga haemolytica, 312 ('apnocylophaga II Medium, 311 Capnocytophaga Medium. 311 Capnocytophaga ochracea, 1576, 1890 Capnocytophaga sj>eeies, 311, 369, 1830 Capnocytophaga sputigena. 1576. 1890 Caprylate Hiallous Agar, 465 Capseilina species, 706 Capsule production, 1914 Carbohydrate assimilation tests. 1937,1938 Carbohydrate Consumption Broth Base with Carbohydrate. 312 Carbohydrate Consumption I liVeg Broth Base with Carbohydrate, 312 Carbohydrate fermentation, 118, 119,312, 313,613,614, 648,672,695,955, 1326, 1336, 1367,1618. 1823. 1932 Carbohydrate Fermentation Broth, 312 Carbohydrate fermentation tests. 1367 Carbohydrate fermenting, 585 Carbohydrate Medium Base. 358 Carbohydrate utilization, 87, 887

Carbohydrates. 672, 1336 Carbon assimilation. 1033 Carbon Assimilation Medium, 313 Carbon Assimilation Medium, Auxanographic Method for Yeast Identification. 313 Carbon Monoxide Oxidizers Agar. Modified, 313 Carbon source, 45, 70,87,91,95, 102, 105. 189, 211, 255, 285, 318,337, 338, 348. 364. 374. 375. 389, 394.395,396. 397, 430,470, 878,998, 1005, 1330, 1470, 1572,1691,1872, 1873, 1874, 1921 Carbon source assimilation, 313 Caibon Utilization Test, 314 Carbonate-Buffered Medium CMB4 with Glucose, 314 Carlwphdus carbo.xidus, 314 Carboxydobacteriiim Medium, 315 Carboxydobrachium pacificum, 316 Carboxydohrachium pacificum Medium, 315 ('arboxydothermus hydrogenoformans, 316 Carboxydothermus Medium, 316 Carboxymcthyl Cellulose Medium., 316 Carcboxydobrachium paci/icum Medium. 1716 Cardiobacterium hominis. 317 Cardiobacterium hominis Medium, 317 Carnation Leaf Agar, 317 Carnitine Chloride Medium, 317 Carnitine Medium for Tondopsis. 318 Carnobacterium alterfunditum. 181. 744, 1853 Carnobacterium divergens, 1234. 1428, 1945 Carnobacterium funditum, 181,744. 1853 Carnobacterium gallinarum, 1428, 1945 Carnobacterium Medium, 318 Carnobacterium mobile, 1428, 1945 Curnoixicterium piscicola. 318. 1428, 1843. 1945 C 'arnobacterium piscicola, 225 Can's Ethanol Medium, 318 Carrot IX'coction Agar. 318 Carrol Potato IXwlrose Agar, 318 Cary and Blair Transport Medium, 318 Cary and Blair Transport Medium, Modified, 319 Caryophanon latum, 22, 319, 761, 933 Caryophanon latum Medium. 319 Caryophanon Medium. 319 Caryophanon species, 319 Caryophanon tenue, 319 CAS Medium. 319 Casamino acids, 4 Casamino Acids and Yeast, 320 Casamino Acids Glucose Medium. 319 Casamino Acids Medium, 320 Casamino Acids Peptone Czapek's Agar, 320 Casamino Acids Yeast Kxtract Broth. 320 Casamino Acids Yeast Fxtract l.incomycin Medium. 320

Index

Casamino Acids Yeast hxtract Sails Broth, Gorbach,321 Casamino Peptone C/apek Medium, 321 Casein Agar, 321 Casein hydrolysatc. 2 Casein Hydrolysatc Yeast Extract HiVeg Broth, 321 Casein 1 lydrolysate Yeast Extract Salts HiVcg Broth Base with Tracer Salts, 321 Casein Medium, 322 Casein Soya Agar, Modified, 322 Casein Soya Agar, Modified with Blood, 322 Casein Soya Peptone Medium, HiVeg, 322 Casein Yeast Extract Glucose Agar. 322 Casein Yeast Extract Glucose Broth, 322 Casein Yeast Magnesium Agar. 322 Casein Yeast Magnesium I liVeg Agar, 323 Casein Yeast Magnesium 1 liVeg Broth. 323 Casitone, 2 Casitonc Agar. 323 Casitone Glycerol Yeast Autolysate Broth. 323 Casitone Glycerol Yeast Autolysate HiVeg Broth Base with Glycerol, 323 Casitone Yeast Extract Agar. 324 Casitone Yeast Extract Glucose Agar, 324 Casman Agar Base, 324 Gasman Agar Base with Rabbit Blood, 324 Casman HiVeg Agar Base with Blood. 324 Casman HiVeg Broth Base with BUXKI, 325 Gasman-Medium. 324 CASO Agar, 325 CASO Bouilion. 325 CASO MUG Agar. 325 Caslenholl/ Agar, Modified, 325 Castenholtz 1) Medium. 325 Caslenholl/ D Medium. Modified. 326 Castenholtz DG Medium, 326 Caslenholl/ DGN Medium. 326 Castenholtz Medium, Modified, 327 Castenholt/ ND Medium, 327 Castenholtz Salts. 4 Caslenhol/. Medium, 327 Castenholz TrypticaseIM Yeast Extract Medium, 327 Caslenhol/ 1YH Medium, 327 Caslenhol/. TYE Medium with 2% Trypticascâ&#x201E;˘ Yeast Extract. 328 CAT Medium,, 328 Catalase production, 939,964 Catellataspora citrea, 1939 ('alellatospora fermginea, 1939 Catellibacterium nectariphilum. 1303 Catenococcus Agar, 329 Catenococcus Medium, 329 Catenococcus thiocyclus, 329 Catenuloplanes japonicus. 1318 Cattle, 808 Caulobacter halobacteroides. 330,1013. 1557 Caulobacter henricii, 255 Caulobacter maris, 330. 1013.1557

Caulobacter Medium, 329, 330 Caulobacter Medium II, 330 Caulobacter Medium with Riboflavin, 330 Caulobacter species, 330,1184 Caulochytriuut protoslelioides. 809 Cautions, 9 CAYKHiVcg Broth. 321 C A YES, 321 CBI Agar. 397 CC Medium, 330 CCD Agar, 331 CCDA, 299 CGDA Selective Supplement, 6 CCDA, Modified, 299 CCFA.404 CCVC Medium, 331 CCY Modified Medium, 332 CDC Anaerobe Blood Agar, 332 CDC Anaerobe Blood Agar with Kanamycin and Vancomycin, 332 CDC Anaerobe Blood Agar with PEA, 333 CDC Anaerobe Blood Agar with Phenylethyl Alcohol, 333 CDC Anaerobe I.akcd Blood Agar with Kanamycin and Vancomycin, 333 CDC Anaerobe I.akcd Blood Agar with KV, 333 CDC Modified McClung-Toabc Egg Yolk Agar, 1035 CDC Modified McClung-Toabc I-gg Yolk Agar. 1035 Cefiximine Rhamnose Sorbitol MacConkey Agar. 334 Cefoperazone Selective Supplement, 6 Ccfopcra/onc Vancomycin Amphotericin Medium, 468 Cefsulodin Irgasan*1 Novobiocin Agar, 337. 388 Celery Decoction Agar, 335 Cell Growth Medium. 335 Cell lines, 619, 620, 621, 1791. 1792, 1793, 1794 Cellobiosc Arginine Lysine Agar, 291 Cellobiosc Arginine Lysine Broth. 291 Cellobiosc Arginine Lysine HiVeg Agar, 292 Cellobiosc Arginine Lysine HiVeg Broth. 292 Cellobiosc Polymyxin B Colistin Agar. Modified. 336 Cellobiosc Polymyxin Colistin Agar, 336 Cellulase Solution, 337 Cellulolytic Agar for Thermophiles, 337 Ccllulolytic Agar with Sea Salts, 337

Cellulolytic bacteria, 338 Ccllulolytic Cellulolytic Ccllulolytic Cellulolytic Ccllulolytic Cellulolytic Cellulolytic

Brolh for 'fhcrmophilcs. 337 Broth with Sea Salts, 337 Clostridia Medium, 338 Clostridium species, 338 Medium. 338 Medium with Rumen I'luid, 338 Medium with Rumen I'luid and

Soluble Starch, 339

1971

Cellulomonas biuzotea. 454 Cellulomonas cartae, 1470 Cellulomonas cellasea. 453,454 Cellulomonas cellulans, 454, 767, 1330 Cellulomonas fennentans. 339 Cellulomonas fennenlans Medium, 339 Cellulomonas fnni. 454 Cellulomonas Jlavigena, 454 Cellulomonas gelida, 454 ('ellulomonas hominis, 1223 Cellulomonas Peptone Tryptone Yeast Extract Glucose Medium, 339 Cellulomonas PI"YG Medium, 339 Cellulomonas species, 339.612.1830 Cellulomonas turhata, 454, 767, 768, 1330 Cellulomonas tula. 454 CeUulophaga balttea, 1833 CeUulophaga fueicota. 1833 CeUulophaga lylica, 471 CeUulophaga uliginosa. 1558

Cellulose Agar, 339 Cellulose Anaerobe Medium, 340 Cellulose Broth, 340 Cellulose degrading, 200 Cellulose Overlay Agar. 340 Cellulose-utilizing bacteria, 75 Cellulosiinicrobium cellulans. 768 Cellvihrio gilvus, 1363 Cellvibrio japonicus. 317 Cellvihrio Medium, 341 Cellvibrio mixtus. 341.611 Cellvibrio species, 611, 612 Cenococcum geophilum, 1199 CENS Medium, 341,342 Ccntcnum Medium. 342 Cenlipeda peiioilonlii, 254 Cephaleuros \irescens. 1412 Cephaliophora irregularis, 1065 Cephalothin Cyclohcximide Vancomycin Colistin Medium. 331 Ceratocystiopsis minuta, 1004 Ceratocystiopsis minutabicolor. 1004 Ceratocystiopsis retusi, 1004 Ceratocystis adiposa. 1348 C 'eratocystis brunnea, 1322 Ceratocystis cana. 1004 Ceratocystis coerulescens, 615, 1348 CeratocystisJimbriata. 1348 Ceratocystis ma/o. 1322 Ceratocystis Medium, 342 Ceratocystis microspora. 615 Ceratocystis multiannulata, 342, 1004 Ceratocystis nigra, 1004 Ceratocystis olivacea, I OOt Ceratocystis olivaceapini. 1004 Ceratocystis penicillata, 999 Ceratocystis perfecta. 1004 ('eratocystis pilifera, 1004 Ceratocystis radicicola. 1322 ('eratocystis seticollis, 1004 Ceratocystis tremuloaurea. 1004 Cercomonas Species, 1589

1972

Index

Cercospora belicola, 809 Cereospora medicaginis, 1410 Cereal Agar. 342 Cetoin production, 1236, 1237 Celraria islandica. 951 Celrimide Agar Base with Glycerol, 343 Cetrimidc Agar Base with Glycerol and Nalidixic Selective Supplement, 343 Cetrimidc Agar, Non-USP. 343 Celrimide Agar, US1\ 343 Cetrimidc HiVeg Agar Base with Glycerol. 343 Celrimide HiVeg Agar Base with Glycerol and Nalidixic Selective Supplement, 343 CF Assay Medium, 344 CFAT Medium. 344 CFC Selective Supplement, 6 CfVFB, 436 CGY. 323 CGY Agar, 344 CGY Autolysate Broth, 313 CH. 1 Medium. 344 Chaemisiphon species. 213, 214 Chaetocladium brefeldii, 999 Chaetomium adiniKladium. 1476 Chaetomium anahelicinum, 340 Chaetomium apieulatum, 340 ('haetomium atrobrunneum, 340 Chaetomium bostrychodes. 999 Chaetomium carinthiaciiin, 340 Chaetomium eoehliodes, 1417 ('haetomium fimicola, 999 Chaetomium globosiim. 16. 340. 587.999, 1417 Chaetomium indicum, 999. 1000, 1004 Chaetomium luknowense, 999 Chaetomium Medium, 345 Chaetomium medusarum. 340 ( haetomium megalocarjmm, 999 Chaetomium murontm, 999 Chaetomium pachypodioides, 999 Chaetomium perlueidum, 999 ( haetomium piluliferum, 1004 Chaetomium quadrangulatum. 340. 999 Chaetomium reflexunu 340, 999 Chaetomium seminudum, 999, 1004 Chaetomium spinasum, 1004 Chaetomium subaffine. 999 ('haetomium suftspirilliferum. 999 Chaetomium suceineum, 999 Chaetomium thielavioideum. 340 Chaetomium trilaterale, 1004 Chaetomium undulatum. 340 Chaetomium variosporum, 340 Chaetomium \irescens. 1476 Chaelospermum chaetosporum, 1000, 1004 Chalaropsis thielavioides. 1410 Chalquist's Antigen Medium, Modi lied, 345 Chapman Stone Agar. 345 Chapman Tellurite Solution, 6 Characium polymorphum. 70 Charcoal Agar, 345

Charcoal Agar Base, HiVeg with Blood and Selective Supplement, 346 Charcoal Agar Slants, 600 Charcoal Agar with Horse Blood, 346 Charcoal Agar with Horse Blood and Ccpahalexin, 346 Charcoal Blood Medium. 347 Charcoal HiVeg Agar Base with Niacin, Blood, and Selective Supplement, 347 Charcoal Yeast Extract Agar. 471 Charcoal Yeast Fxtiact Agar, Buffered, 471 Charcoal Yeast Fxtract Diphasic Blood Culture Medium, 942 Chase's Medium. SP. 347 CHCA Salts Medium, 347 Cheddar cheese. 986 Cheese, 274,986, 987,1585, 1586 Cheese Agar. 348 Chelatobacter heintzii 1294, 1402 Chelatococeus saeeharophobus. 1294. 1402 Chemostat culture, 1043 Chemolherapeutic agents, 611 Cherry Agar, CBS Formula, 348 Cherry Decoction Agar, 348 CHI, 1776 Medium. 348 Chicken Soup Broth. 348 China Blue Lactose Agar, 349 China Blue lactose HiVeg Agar. 349 Chitin Agar. 349 Chitinophaga pinensis, 324, 1218 Chlamydia Growth Medium, 350 Chlamydia Isolation Medium, 350 Chlamydia species. 350, 351. 1653.1654 Chlamydomonas applanata, 1588 Chlamydomonas dorsoventralis. 70 Chlamydomonas Fnriched Medium, 351 Chlamydomonas hedleyi, 822 Chlamydomonas Mutant Agar, 351 Chlamydomonas Mutant Broth. 351 Chlamydomonas provasolii, 822 Chlamydomonas reinhardtii, 351, 1070. 1071, 1072 Chlamydomonas species, 153 Chlamydospore Agar, 351 Chlamydospore formation, 351, 1513, 1514 Chlamydospore production. 480 Chlamydospores. 451,711. 1799. 1913 Chloramphenicol. 1530 Chloramphenicol Ampieillin I,B Medium, 351 Chloramphenicol Frythromycin I.B Medium, 352 Chloramphenicol I. Broth Medium No. 1, 352 Chloramphenicol L Broth Medium No. 2, 352 Chloramphenicol L Broth Medium No. 3, 352 Chloramphenicol LB Medium No. 2. 352 Chloramphenicol LB Medium No.l, 352 Chloramphenicol Selective Supplement, 6 Chloramphenicol Yeast Glucose Agar, 353

Ch/urella Agar, 353 Chlorella Broth, 353 Chlorella Broth without Glucose and Citrate. 353 Chlorella ellipsoidea. 70 Chlorella luteoviridis, 70 Chlorella miniala, 70 ('hlorella protothecoides, 70 Chlorella pyrenoidosa. 1617. 1618 Chlorella saceharophila, 70, 822 Chlorella species. 70. 353 Chlorella variegata. 70 Chlorella vulgaris. 70 ('hlorella xanthella, 70 Chlorella zopflngiensis. 70 Chlorinated Fatty Acid Medium. 354 Chlorinated phenols, 1815 Chlorinated water. 1682 Chloroacrylic acid utilizing hacteria, 1045 Chlorobcnzoatc utilizing bacteria. 1174 Chlorobiaceae, 354, 355, 378. 1014 Chlorobiaceae Medium, 1. 354 Chlorobiaceae Medium, 2, 354 Chlorobium ferrooxidans, 1046 Chlorobium limtcola, 355, 383, 1379 Chlorobium phaeobacteroides. 1380 ('hlorobium phaeovibrioides, 1379 Chlorobium thiosulfatophilum Medium. 355 ('hlorobium vihrioforme, 1380 Chlorobulane Medium. 355 Chloroflexus Agar. 355 Chloroflexus aggregans. 356 Chloroflexus aggregans Medium, 356 Chloroflexus aurantiacus. 356. 357 Chloroflexus Broth. 356 Chloroflexus Medium, Modified, 357 Chloroflexus species, 326 Chlorohydroxyben/oic Acid Medium, 357 Chlorosphaera klelxsii. 70 CHO HiVeg Medium Base with Carbohydrate Solution, 357 CHO Medium, 672 CHO Medium Base, 358 ( hoanephora infundibulifera, 809 Choanoeca perplexa. 1015 Chocolate Agar, 358 Chocolate Agar Base with Hemoglobin and Vitamino Growth Supplement. 360 Chocolate Agar Base with Hemoglobin and Yeast Autolysate. 360 Chocolate Agar, Fnriched, 358, 359 Chocolate Agar-Bartonella C-29.288, 359 Chocolate HiVeg Agar Base with Hemoglobin and Vitamino Growth Supplement, 362 Chocolate HiVeg Agar Base with Hemoglobin and Yeast Autolysate. 361 Chocolate II Agar, 360 Chocolate 11 Agar with I Icmoglogin and IsoVitaleX速, 361 Chocolate No. 2 Agar Base widi Hemoglobin, 360,361

Index

Chocolate No. 2 Agar Base with Supplements, 360 Chocolate No. 2 IliVeg Agar Base with Hemoglobin, 362 Chocolate syrup, 1413 Chocolate Tellurite Agar, 361 Cholera cntcrotoxin. 1923 Cholera I HVcg Medium Base with Tellurite and Blood, 363 Cholera Medium Base with Tellurite and Blood.363 Cholera Medium TCBS. 363 Cholesterol Medium, 364 Cholic Acid Medium, 364 Choline Assay Medium, 364 Choline Medium, 365 ( hondrococcus maenxsporus, 1272 Chondromyces species, 365 Chondromyces VYZ Agar, 365 Chopped Liver Broth. 365 Chopped Liver HiVeg Broth, 365 Chopped Meat Agar, 365 Chopped Meat Broth, 365 Chopped Meat Broth with Carbohydrates. 366 Chopped Meat Broth with Formate and Fumaratc, 366 Chopped Meat Broth with Vitamin K,, 43, 366 Chopped Meat Carbohydrate Medium. 367 Chopped Meat Carbohydrate Medium with Rumen Fluid, 367 Chopped Meat Carbohydrate Medium with Tween 80, 367 Chopped Meat Glucose Agar. 368 Chopped Meat Glucose Broth, 368 Chopped Meat Glucose Medium, 368 Chopped Meat Glucose Medium with NaCl, 368 Chopped Meat Medium, 369 ChopjHxl Meal Medium for Treponema spp., 373 Chopped Meal Medium with 0.025% Tween 80, 373 Chopped Meat Medium with 1% Glucose, 370 Chopped Meat Medium with 1% Tween 80, 374 Chopped Meat Medium with 10% Fetal Calf Serum, 369 ChopjKxl Meat Medium with 10% Reduced Filtered Rumen Fluid. 373 ChopjHxl Meat Medium with Formate and Fumaralc. 369 Chopped Meal Medium with Menadione. 370 Chopped Meat Medium. Modified, 370,371 Chopped Meat Medium. Modified with Arginine, 371 Chopped Meat Medium. Modified with Formate and Fumarate, 372

Chopped Meat Medium, Modified with Tween 80, 372 Chorogioeopsis species. 213. 214 Christensen Agar, 374 Christcnscn Citrate Agar, 374 Christensen Citrate Agar, Modified, 374 Christcnscn Citrate Sulfide Medium. 375 Christensen Citrate Sulfite Agar, 375 Christcnscn HiVeg Agar Auloclavable, 1870 Christensen's Urea Agar, 375 Christensen's Urea Agar with Sodium Chloride, 375 Christopher's Semisolid Brucella Medium Base, 376 CIlROMagar Candida, 376 CIlROMagar ÂŁ. coli. 376 CI IROMagarHCC, 376 CI IROMagar Listeria, 376 CI IROMagar Mulassezia, 376 CI IROMagar MRSA. 376 CI IROMagar 0157,377 CI IROMagar Orientation, 377 CIlROMagar Pseudomonas, 377 CI IROMagar Salmonella, 377 CI IROMagar Staph, aureus, 377 CIlROMagar StrepB, 377 CIlROMagar KAno,378 CIlROMagar VRE, 378 CIlROMagar 1 "Salmonella Plus, 377 Chromatiaceae, 379, 1014 Chromatiaccac Medium. 1. 378 Chromatiaceae Medium, 2, 378 Chromaliwn gracile. 1377 Chromutiwn Medium, 379. 380,383 Chromatium Medium with Sodium Chloride. 381 Chromatium salexigens, 382 ('hromatium salexigens Medium, 382 Chromatium species, 380. 383.800 Chromatium tepidttm. 380, 383 Chromatium tepidum Medium, 383 ('hromatium vinosum, 1926 Chromatium-Thiocapsa Medium, 383 Chtvmolxicterium marismortui. 799 Chmmohacterium Medium, 383 Chromolxicterium species. 384 Chromohaelerium violaceum, 1250 Chromohactertium species. 1304 Chromocult* Coli form Agar, 437 Chromocult* Coliform Agar F!S, 437 Chromocult'*' l-nhanced Selectivity Agar, 437 Chromocult* Kntcrococci Broth. 648 Chromocult* TUX, 1837 Chromocult*"Tryplone Bile X-glucuronide Agar, 1837 Chromogenic Bacillus cereus Agar, 257 Chromogcnic Candida Agar. 384 Chromogenic /â&#x20AC;˘'. cW//Colifonn Agar, 258 Chromogenic 1:. co///Coliform Medium, 384 Chromogenic I'.nterohacter sakazakii Agar, DF1 Formulation. 384 Chiomogenic Listeria Agar, 384

1973

Chromogenic Listeria Agar (ISO). 385 Chromogenic Listeria Agar (ISO) Modified, 385 Chromogenic Salmonella Esterase Agar, 385 Chromogenic Substrate Broth. 386 Chromogenic Urinary Tract Infection (UTI) Medium. 386 Chromogenic UTI Medium, Clear. 386 Chromohalobacter marismortui, 1554 Chroococcidiopsis species, 213, 214 Chroomonas salina. 822 Chryseohaelerium spp., 1830 Chryseomonas luteola, 1304 Chrysiogenes arsenatis, 387 Chrysiogenes Medium. 387 Chrysosporium faslidium, 1935 Chrysosporium pannorum, 340 Chrysosporium xerophilum. 1332, 1935

Chu's Medium No. 10,387 Out's No. 10 Medium. 387 Chu's No. 10 Medium. Modified, 388 Clufs No. 11 Medium. Modified, 388 Ciboriopsis simulata, 1004 Cidiphilium acidophilum. 1752 ('iliophrys infusionum, 1556 CIN Agar. 334.388 ("inefobaeler spp., 1824 Cinnamate Medium, 388 GBP Medium, 89, 1269 Circinella umbeliata, 1065 Cirrenalia macrocephala. 1004 Citeromyces matrilensis, 1909 Citrate Agar, 374.1572 Citrate A/ide F.nterococcus IliVeg Agar Base. 290 Citrate A/ide Tween Carbonate Base, 389 Citrate Medium. Koser's Modified. 389 Citrate I'hospltate Buffered Glucose Medium. 389 Citrate utilization. 62,1572 Citrobacter diversus. 390 Citrobacter diversus Medium, 390 Citrobacter freundii, 1330 Citrobacter koseri. 1330 Citrovorum factor assay, 344 Citrovonim Factor Assay Medium, 344 Citrus juice, 1329 Citrus products, 1329. 1330 CK Agar, 390 CK1 Medium. 390 CI, 1459 Cladonia species. 951 Cladosporium eladosponoides, 585, 586, 587,742 Cladosporium cucumerinum, 585, 586, 587, 742 ('iadosporium fulvum, 1410 Cladosporium herbarum, 585, 586. 587. 742 (Iadosporium macroearpum, 585, 586. 587, 742 Cladosporium sphaerospermum, 585. 586. 587, 742

1974

Index

Cladosporium urediniculu. 585. 586, 587. 742 Cladosporium vignae. 1641 Clausen IliVeg Medium, 390 Clausen Medium. 391 Ciavibacier imnicum, 1368 Clavibacter Medium, 391 Ciavibacier michiganense, 391, 454, 1368, 1928 ('lavibacler michiganensis, 454,455, 1428 Clavibacter michiganensis Medium. 392 Clavibacter michiganensis subsp. sepedonicus, 392 Clavibacter ralhayi, 1368

Clavibacter species. 1428 Clavibacter loxicus. 604, 1054 Clavibacter tritici, 1368 Clavibacter xyli, 454. 1543, 154ÂŤ Clavibacter xyli subsp. cynodonti. 1543 ('lavicorona divaricata, 392 Clavicorona Medium. 392 Clavicorona pyxidata. 392 CLED Agar. 392 C L E D Agar with Andrade's Indicator, 392 Clinic. 1650. 1651 Clinical specimens, 61, 164,191. 192,201. 202, 203,204, 205,237, 238,244,245, 246. 247. 267, 269.272. 281.297, 319. 332,333,334,344,360,361,388,404, 435.439.440. 442.443.444.468. 586. 613. 666.667. 703.719. 720. 729. 730, 754, 760, 761, 810,817, 818,892, 903, 909.910.931.940.943.991.992. 1006. 1032,1033, 1202, 1215, 1231, 1233, 1260.1268, 1283, 1287,1305, 1373. 1396.1397, 1423. 1480, 1485, 1486. 1487,1527, 1528, 1531, 1559, 1630, 1633.1634, 1644, 1669,1687, 1772, 1775, 1825, 1827. 1828, 1829, 1830. 1831, 1836, 1851,1865, 1866, 1877, 1931,1943 Clostridia Medium, 393 Clostridial Agar. 393 Clostridial IliVeg Agar, 393 Clostridiisalibacter paucivoran. 1897 Clostridium acelicttm, 34 Clostridium acelireducens, 1483 Clostridium acetobutylicum, 393. 1456, 1757 Clostridium aceiobtttylicttm Medium, 393 ( lostndium acidisoli, 403 Cioshidium aciduria. 394. 1488. 1873. 1874 Clostridium acidurici Medium, 393 Clostridium aerotoierans, 394 ('lostridium aerotoierans Medium, 394 Clostridium akagii. 403 Clostridium aldrichii. 394, 395, 430 Clostridium aldrichii Agar. 394 Clostridium aldrichii Hroth, 395 Clostridium Alginate Medium, 395 ('lostridium alginolyticum, 395 Clostridium alkalicellum Medium,, 395 Clostridium alkalicellum. 396

Clostridium aminobutyricum. 396, 397 Clostridium aminobutyricum Medium, 396 Clostridium aminophilum, 1483. 1487 ('lostridium aminovalericum, 366, 397 Clostridium aminovalericum Medium, 397 Clostridium auranlibutyTicum, 366 Clostridium Ixtratii, 366 Clostridium barkeri, 209 Clostridium beijerinckii. 366.393.397.1459. 1844 Clostridium beijerinckii Agar. 397 Clostridium beijerinckii Broth, 397 Clostridium botulinum, 365, 398, 960, 1035, 1349,1350.1618, 1619. 1823,1824, 1850 Clostridium botulinum Isolation Agar. 397 ('lostridium botulinum Isolation 1 liVeg Agar, 398 Clostridium Broth Base. 398 Clostridium bryantii Medium, 399 Clostridium butyricum. 366. 393.880. 1459, 1845 Clostridium cadaveris. 366 ('lostridium caminithermalis Medium. 400 Clostridium caminilhennalis. 400 Clostridium carnis, 366 Clostridium celatum. 366 Clostridium celerecrescens, 425,429, 430 Clostridium cellobioparum. 401 Clostridium cellobioparum Agar, 400 Clostridium cellobioparum Broth, 401 Clostridium cellobioparum Medium, 401 Clostridium Cellulolytic Medium. 401 Clostridium cellulolyttcum, 402, 430 Clostridium Cellulose Medium, 402 Clostridium cellulovorans, 102. 402 Clostridium celluloutrans Medium, 402 Clostridium chartatabidum. 403 Clostridium chartatabidum Medium, 402 Clostridium chaitvoei. 403 Clostridium chaitvoei Blood Agar, 403 Clostridium CK Medium. 403 ( lostridium closlridiiforme, 366 Clostridium cocleatwn, 1668 Clostridium colinum. 1890 Clostridium cylindrosporum. 1873. 1874 ( lostridium difficile, 404,470, 1890 Clostridium difficile Agar. 403.404 Clostridium difficile IliVeg Agar Base, 404 Clostridium difficile Selective Supplement, 6 Clostridium eslertheticum. 404 Clostridium eslertheticum Medium, 404 Clostridium felsineum. 1805 Clostridium/ormicoacelicum, 405 Clostridium formicoacelicum Agar, 405 Clostridium formicoaceticum Broth, 405 Clostridium frigidicarnis, 1487 ('lostridium granfii, 406 Clostridium granfii Medium. 405 Clostridium haemolyticum, 332 Clostridium halophilum. 407,411.414 ('lostridium halophilum Medium, 406

Clostridium histolyticum, 407, 940 Clostridium histolyticum Medium, 407 Clostridium IliVeg Broth Base with Lactate. 407 Clostridium homopropionicum. 715, 717 Clostridium homopropionicum DSM 5847, 1204, 1205 Clostridium hydroxybenzoicttm. 408 Clostridium hydroxyhenzoicum Medium, 407 Clostridium kluywri, 408 Clostridium kluywri Agar. 408 Clostridium kluywri Medium. 408 Clostridium iaclatifermentans, 246 Clostridium laniganii, 1417 Clostridium lenlocellum, 409, 430 Clostridium lentocellum Agar. 408 Clostridium litorale, 409,411,414 Clostridium litorale Medium. 409 ('lostridium ljungdahlii, 410 Clostridium ljungdahlii Medium. 409 Clostridium longisporum, 410 Clostridium longisporum Medium. 410 Clostridium M1 Medium, 413,414 Clostridium magnum. 765 ('lostridium mayombei, 44 Clostridium Medium. 410. 411,412 Clostridium melhoxybenzovorans, 660 Clostridium methylpentosum. 415,616.617 Clostridium methylpentosum Medium, 414 Clostridium neopropionicum. 415 Clostridium neopropionicum Medium, 415 Clostridium noterae, 416 Clostridium noterae Medium, 415 Clostridium novyi. 416, 940 Clostridium noxyi Blood Agar, 416 Clostridium oroticum, 416, 1331, 1955 Clostridium oroticum Medium. 416 Clostridium oxalicum, 107 Clostridium papyrosolvens, 337.338. 417. 425,429 Clostridium papyrosolvens Medium. 416, 417 Clostridium paradoxum. 1953 Clostridium peptidivorans DSM 12505, 824 Clostridium perfringens. 60. 105. 106,284. 365,417, 613, 672, 673,840, 880. 929, 931.940.958.1034.1035. 1036.1066, 1201.1203,1225. 1370.1569.1618. 1619, 1772. 1824. 1851,1852, 1853 Clostridium perfringens Agar, OPSP. 417 Clostridium perfringens Sanitation Broth, 417 C 'lostridium perfringens Sporulation I IiVeg Broth, 417 Clostridium pfennigii, 418 ('lostridium pfennigii Medium. 417 Clostridium polysaccharolyticum, 339. 401 ('lostridium populeti, 430 Clostridium propionicum. 418 Clostridium propionicum Medium, 418 Clostridium puniceum, 1426 Clostridium purinolyticum. 394

Index

Clostridium quinii, 767 Clostridium roseum, 393, 1845 Clostridium saccharobutylicum. 1487 ('lostridium saccharolyticum, 1074 Clostridium saccharoperbutylacetonicum, 1932 Clostridium Selective Agar. 418 ('lostridium septicmn, 940 Clostridium sordelli, 940 Clostridium sp. I)SM 11261, 1211 Clostridium species. 40.100. 104. 117. 198. 207.225. 367, 368, 369, 371, 393,397, 409.410.411,412.413.418.429.430. 460. 598, 632. 633, 678. 718, 732. 748. 765, 880, 929. 939,940, 958,963, 1034, 1369. 1453.1459. 1483.1486. 1487. 1533.1534. 1714,1814.1850.1859. 1863 Clostridium sphenoides. 419.448. 952 Clostridium sphenoides Medium, 419 Clostridium spiroforme. 1668, 1890 Clostridium stercorarium, 337, 425 Clostridium sticklandii, 419 Clostridium slicklcmdii Medium, 419 Clostridium symbiosum. 254 ('lostridium tepidiprofundi, 1693 Clostridium termitidis. 282.420 Clostridium termitidis Medium, 419 Clostridium tbermoaceticum. 421, 422. 1074 Clostridium thermoaceticum II Medium, 422 Clostridium thermoaceticum Medium. 420, 421 Clostridium thermoalcaliphilum, 1953 Clostridium fhennoamylolyticum. 451. 453 Clostridium thermoautotrophicum. 32, 1730 Clostridium thennocellum, 423, 424,425, 429.762 Clostridium thennocellum Medium, 423,424 Clostridium thermohvdrosulfuricum, 425, 1074 C lostridium thermohydrosulfuricum Medium, 425 Clostridium thermolacticum, 425 Clostridium thermolacticum Medium. 425 ('lostridium therinoptdmarium. 430 Clostridium thermosaccharolyticum, 425 ('b/stridium thermosuccinogenes, 426 Clostridium thermosuccinogenes Medium. 425 Clostridium ihermosulfurogenes, 967 Clostridium thermosulfurogenes Medium, 426 Clostridium thiosulfatireducens DSM13105, 825 Clostridium tyrobutyricum. 399. 407 ('lostridium uliginosum, 403 Clostridium ultunense. 427 ('litslridium ultunense Medium, 427 Clostridium uzonii. 1338 Clostridium vineentii, 428 Clostridium vineentii Medium, 427 Clostridium welchii, 1907

Clostridium xylanolyticum. 1459, 1921 Clostridium xylanovorans, 823 Clostrisel Agar. 418 CM, 437, 438 CM + YE Medium B, Modified, 431 CM, 3 Agar. 428 CM. 3 Broth, 429 CM Agar, 428 CM plus YE Agar. Modified, 430 CM plus Yi: Medium, 431 CM I Broth Powder. 4 CM3 Medium. 429,430 CM4 Medium. 430 CMA, 451 CMA with Lupine. 431 CMA witli Stenle Carrot, 431 CMI.Malium.43l CMRL 1066 Medium with Glutamine. 10X. 432 CMYG. 453 CN Inhibitor, 6 CN Screen Medium. 432 CNS Agar, 432 CNV Antimicrobic. 6 CNVT Antimicrobic, 6 Coagulase Agar Base. 433 Coagulase Mannitol Agar, 433 Coagulase Mannitol Broth Base with Plasma. 433 Coagulase Mannitol HiVeg Agar Base with Plasma, 437 Coagulase Mannitol HiVeg Broth Base with Plasma. 434 Coagulase production. 433,434 Coagulase-posilive. 192. 193, 194,657 Coagulasc-positive staphylococci, 192. 193, 194,1691 Coal Medium, 434 COBA, 434 Cobetia marina. 1009 Cocci, 442, 443,444. 473, 1384 Coccidiodes immiti.s. 245. 254,447. 1928 Coccidioidal and Histoplasmin Broth, 153 Coccidoidin. 153 ('ochliobolus australiensis, 1877 Cochliobolus bicolor, 1877 Cocbliobolus miyalwanus, 1877 Cochliobolus ravenelii. 1348 Cochliobolus sativus, 1286, 1641, 1877 Cochliobolus spicifer, 1877 Cochliobolus victoriae, 1641, 1877 ('ochliopodium actinophora, 706 Cochliopodium bilimbosum. 706 Cochliopodium clanim, 1011 Codinaea simplex, 588. 615 ('odasiga gracilis, 1015 Coelastrum proboscideum. 70 Colbeck's Egg Broth, 6 Colby and /atman Agar. 435 Colby and Zatman Medium. 435 Colby and /atman Thiamine Malium, 435 Cold Filterable Tryptone Soy Broth, 435

1975

Cold Filterable TSB, 435 Cold Filterable Vegetable Peptone Broth. 436 Coleosporium tussilaginis. 1% Colelsos Medium, 436 Coletsos Selective Medium, 436 Coli ID, 437 Colilbrm, 261, 263. 386.634.636. 637. 640. 641, 642, 643, 669, 670, 701,709, 932, 939, 950.1031.1032, 1066,1067. 1150. 1186, 1196,1427, 1590, 1626, 1690, 1694, 1840,1886,1894 Colilbrm Agar HS. Chromocult. 437 Conform Agar, Chromocult*, 437 Coliform bacteria. 929. 991, 1186 Colilbrm HiVeg Broth, 437 Coliform Malium. 437. 438 Colilbrm Malium, Modi lied, 438 Coliform microorganisms. 437,438. 671 Colilbrm PA Broth, 438 Coliform Rapid IliCromc 1 " Broth, 843 Coliforms, 257,374,375,384,386,437,439, 623.833.834, 837.841,928.961.990. 991, 992, 993, 994, 998. 1066. 1151, 1252.1253,1480. 1481. 1694, 1695. 1837 Colistin Oxolinic Acid Blood Agar, 434 Cotletotrichum capsici. 1418 Colletotrichum coccodes, 1418 Colletotrichum crassipes. 1418 Colletotrichum dematium, 1418 Colletotrichum gloesporioides, 1418 ('olletofrichum orbiculare, 1322 Colletotrichum species. 952 Collimonas Medium, 439 Collimonas species. 439 Colloidal Chitin Agar, 439 Colonization Medium, 439 Colonization Medium with Dulbeccos Phosphate Buffer, 439 Colony characteristics. 392 Columbia Agar. 439 Columbia Blood Agar, 439,440 Columbia Blood Agar Base, 440 Columbia Blood Agar Base with 1% Agar. HiVeg with Blood, 440 Columbia Blood Agar Base with Horse Blood, 440,441 Columbia Blood Agar Base with I lorse Blood and Charcoal. 441 Columbia Blood Agar Base with Rabbit Serum, 441 Columbia Blood Agar Base, HiVeg with Blood, 440 Columbia Broth. 441. 442 Columbia Brotfi Base. 1 liVcg witli Blood. 442

Columbia Broth Base, HiVeg with SPS, 442 Columbia CNA Agar, 442 Columbia CNA Agar Base with 1 % Agar and Blood. 443 Columbia CNA Agar Base with Blood, 443 Columbia CNA Agar. Modified with Sheep Blood. 443

1976

Index

Columbia CNA HiVcg Agar Base with 1% Agar, 443 Columbia CNA HiVcg Agar Base with Blood.444 Columbia Colistin Nalidixic Acid Agar, 442 Cotwella psychroerythms Medium, 444 Colwellia maris, 1469 Comamonas acidovorans, 596, 1173, 1304, 1330,1445 Comamonas nitrativorans. 215 Comamonas testosteroni, 211, 863, 864, 1057.1304. 1313 Complex Medium. 444 ComiKtsilion of media, 1 ('ondiobulus obsamis, 1948 Confirmatory tcsl. 648, 649 Congo Red Acid Morpholinepropanesulfonic Acid Pigmentation Agar. 444 Congo Red Agar. 444.445 Congo Red Bill Agarose Medium, 445. 446 Congo Red Magnesium Oxalate Agar, 446 Conidia, 1586 Conidiobolus apiculatus, 1460 Conidiobolus obscurus. 1460 Conidiobolus stromoideus, 898 ('oniella pulcheila, 615 Coniochaeta exlrannuulana. 944 Coniochaetidium ostreum. 944 Coniothyriumfuckelii. 952 Connaught Medical Research laboratories Medium with Glutaminc. 10X. 432 Conradi Drigalski Agar. 446 Converse Liquid Medium, I.evine Modification. 446 Cook's Cytophaga Agar. 449 Cook's Cytophaga Agar for Lysobacter, 449 Cooke Rose Bengal Agar, 447 Cooked Meat Liver Medium. 431 Cooked Meat Medium. 71,447 Cooked Meal Medium with Glucose, Ilemin and Vitamin K. 447 Cooked Meat Medium with Glucose, Yeast Extract, and Cysteine. 448 Cooked Meal Medium with Peptone and Yeast l-lxtract, 448 Cooked Meat Medium, Modified. 448 Coprinus cinereus, 449 Cophnus Medium, 449 Coprinus velox, 944 Coprinus xanlhothrix. 931 Coprococcus eulastus, 370 Coprococcus species. 368, 618 Coprothermobacterproleolyticus, 450,457 ('oprolhermobacter proteolvticus Medium, 449 ('oriohactehum glomerans, 1459 Com Meal Agar. 450.451 Com Meal Agar with Dextrose. 452 Com Meal Agar with Glucose, 450 Com Meal Agar with Polysorbate, 80,452 Com Meal Agar with Soil Extract, 452

Com Meal Agar with StreplOO and 1 etlOO. 452 Com Meal HiVcg Peptone Yeast Agar. 450 Corn Meal Phvtophthora Isolation Medium No. 1.452 Com Meal Phytophthora Isolation Medium No. 2.452 Com Meal Yeast Glucose Agar, 453 Com Milk Medium, 450 Com Oil Medium, 450 Com Steep Liquor Medium, 450 Com Steep Starch Nutrient Agar, 451 Com stunt, 980 Cornmeal Agar with Dextrose, 451 Commcal Agar with Polysorbate, 80, 451 Commcal Agar. Quarter-strength, 452 Cornmeal and V8 Juice Agar, 451 Cornmeal Phytophthora Isolation Medium No. 1,452 Commcal Yeast Kxtract Scawatcr Agar, 453 Cornstarch Soluble Medium, 453 Cortinarius species. 1199 Corynebacterium Agar, 453, 454 Corynebacterium Agar with Blood, 454 Corynebacterium Agar with Salt. 454 Corynebacterium ammoniagenes, 453. 454 Corynebacterium amycolatum. 1428, 1932 Corynebacterium hovis, 454 Corynebacterium Broth, 454 Corynebacterium callunae, 45.3, 454 Corynebacterium cystitidis. 1428 Corynebacterium dipbtheriae, 363,455,474, 478. 587.855.901.902.962.963,966. 1251,1382,1476,1788 Corynebacterium dipbtheriae Virulence Test Medium. 901 Corynebacterium durum, 1830 Corynebacterium equi, 1274 Corynebacterium faisenii, 1830 Corynebacterium flavescens, 454 Corynebacterium genitalium, 1834, 1842 Corynebacterium glucuronolylicum, 1830 ('orynebacteriumglutamicum, 453,454,934, 1312,1315,1452.1868. 1869 Corynebacterium herculis, 1312. 1315 Corynebacterium hoagii. 454 Corynebacterium jeikeium, 1428 Corynebacterium kutscberi, 454 Corynebacterium liquid Enrichment Medium, 454 Corynebacterium matrttchotii, 1428. 1848 Corynebacterium Medium CH, 455 Corynebacterium Medium with Blood. 455 Corynebacterium Medium with Salt, 455 Corynebacterium minutissimum. 214 Corynebacterium myeetoides, 454, 1428 Corynebacterium pilosum. 1428 Corynebacterium pseudodiphtheriticum, 1330 Corynebacterium jxseudogenitalium, 1842 Corynebacterium pseudotuberculosis, 816 ( orynebacierium renale, 454

Corynebacterium sepedonicum, 1054 Corynebacterium singulare, 1830 Corynebacterium species, 84.215,270. 355. 363,441, 454,455, 610,813, 852, 941, 1047.1250.1309. 1339.1363.1428, 1464, 1566,1636, 1647, 1704, 1899 Corynebacterium urealyticum, 1428 Corynelxicterium wginale, 1033, 1635 Corynebacterium variabilis, 454 Corynebacterium vitarumen, 454 Corynebacterium xerosis, 1330. 1428. 1830 CorynelHiclrium alkanolyticum, 752

Coryneform bacteria, 1548 Cosmetic products, 98. 948, 1156.1157. 1215 Cosmetics. 108.810. 1001.1224,1683.1684 Costem's EDS Test Medium, 455 Couchioplanes caeruleus subsp. caeruleus, 1318 Cow Manure Agar, 456 CP Medium, 456 CP Medium for Coprothermobacter proteolytievs, 456 CP Medium for lliermobacteroides leptaspartum, 457 CPC Agar, 336 CPC Agar Base with Ccllobiosc. Colistin. and Polymyxin B, 457 CPC HiVcg Agar Base with Ccllobiosc, Colistin. and Polymyxin B. 458 CPC Medium, 458 CR Agar, 444. 445 Craig's Medium, 458 CRAMP Agar. 444 CRAMP I liVeg Agar Base, 458 CRBHO Medium. 446 CREA, 458 Cream. 438 Creatine Agar, 458 Creatinine Agar, 459 Creatinine Medium, 459 Crcatininc/NMI 1 Medium. 459 CreDml Medium, 460 Crinalium epipsammum. 214 Crifhidia acanthocephali. 461 Crithidia deanei, 461, 600 Crithidia fasciculata, 461, 1855 ( Yithidia flexanema, 600 Crithidia harmosa, 461 Crithidia humeri, 461 Crithidia luciliae. 461 Crithidia Medium, 461 Crithidia mellijicae. 461 Crithidia oncopelti, 461, 763 Crithidia species. 461

CRMOX Agar, 446 Crosslcy Milk Medium, 461 CR-SMAC Agar Base, 334 Crucigenia apiculata, 70 Cncelia marina. 1556 Cryobacterium psychrophilum, 1513 Cryphonectria gyrasa, 1413

Index

Cryphoneclria parasitica, 643, 1413 Crypthecodinium cohnii, 59 Cryptoanaerobacler Medium,. 461 Cryptoanaerobacler sjiecies, 462 Cryptococcus albidosimiiis. 749 Cryptococcus a Ihidus, 749, 1909 Cryptococcus curvalus, 1001 ('ryptococcus elinovii, 1001 Cryptococcus laurentii. 749 Cryptococcus neoformans, 223, 291, 432, ' 754.878. 1799.1942 Cryptococcus neoformans Screen Medium, 432 Cryptococcus species. 223, 291. 1950 Cryptococcus vishniacii, 749 Cryptosporiopsis abietina. 317 CRYS Medium, 462 Crystal Violet Agar, 463 Crystal Violet A/ide Esculin Agar, 463 Crystal Violet Esculin Agar. 463 Crystal Violet lactose Agar, 463 Crystal Violet lactose Broth. 464 Crystal Violet Iujctose HiVeg Agar, 464 Crystal Violet Pectale Medium, 464 Crystal Violet Tetrazolium Agar Base, 464 Crystal Violet Tetrazolium HiVeg Agar Base. 464 CS Vitamin B12 Agar. 465 CSE Agar, 385 CSSM, 453 CT Agar. 465. 466 CI Broth,466 CT Medium. 466 CIA Agar, 466 CTA Medium, 466 CTA Medium with Yeast Extract and Rabbit Scrum. 466 CTLM Medium, 467 CTF Medium, 467 Cultivation Medium for Chlamydiae, 467 Cup plate method, 465 Curtobacterium albidum. 454, 1470 Curtohacteriuni citreum, 454, 1470 Curtobacterium Jlaccumfaciens. 278, 453. 454, 1280, 1309, 1368, 1470,1830 Curtobacterium insectiphilium, 1470 ('urtobacterium luteuin, 454, 1470 Curtobacterium psychrophilum. 1513 Curtobacterium pitsiltum, 454, 1470 Curtobacterium species, 1470 ('urvularia inaequalis, 1877 Curvularia lunala, 1476 Curvularia pallescens. 1935 CVA Enrichment, 4 CVA Medium. 468 CVP Medium, 464 CY Agar. 468 CYA Agar, 482 CYA Agar with Arginine and />Aminobenzoic Acid. 468 Cyanide production, 96 Cyanide resistance, 1221

Cyanobactena. 151.213.326.327.387.388, 1428, 1900 Cyanuric acid utilizing bacteria, 1175 Cyalhus species, 263 CYC Agar, 469 CYC Medium, 469 CYC Medium, Cross and Attwell Modification, 469 Cyclidium species, 1589 ('yclobacteruun marinus, 1556 Cycloclasticus pugetii. 1010 Cyclohexane Carboxylic Acid Salts Medium, 347 Cyclohexanecarboxylic Acid Agar. 469 Cyclohexanecaiboxylic Acid Broth, 469 Cyclohexanecarboxylic Acid Medium. 470 Cyclohexanone Medium, 470 Cycloheximide Agar. 54 Cycloheximide Chloramphenicol Agar, 1260 Cycloserine Cefoxitin Egg Yolk Fnictosc Agar, 470 Cycloserine Cefoxitin Fructose Agar. 404 CYE Agar, 471 CYE Agar, Buffered. 471 CYE Broth. 320 CYE-ACES Agar, 470 CYE-DBCM.942 CYG Agar. 322.472 CYG Broth. 322 Cylinder plate technique, 122, 127, 131 Cylindrocladium couratariae. 944 Cylindrocladium Isolation Medium, 472 Cylindrocladium species, 472 ('ylindrodendrum album, 1438 Cylindrospermum species. 213.214 CYM, 472 CYM Medium, 472 CYS Medium,, 472 Cysteine Sulfide Reducing Agent, 4 Cysteine Tellurite Blood Agar, 474,478 Cystinasc production. 1399 Cystine I leart Agar, 473 Cystine Heart Agar with Rabbit Blood, 473 Cystine HiVeg Agar Base with Hemoglobin. 473 Cystine lactose Electrolyte Deficient Agar. 392 Cystine lactose Electrolyte Deficient Agar with Andrade's Indicator, 392 Cystine Tryptic Agar. 466 Cystine TrypticascIM Agar Medium, 466 Cystine Trypticascâ&#x201E;˘ Agar. 466 Cystine Trypticaseâ&#x201E;˘ Agar Medium with Yeast Extract and Rabbit Serum, 466 Cystine Tryptone Agar, 474 Cystine Tryptone Agar, I liVeg, 474 Cystine Tryptone Agar. HiVeg with Carbohydrate, 474 Cystoiyacterfuscus. 1594 Cyslojilobasidium capttatum. 196 CYT Agar. 475 Cytopathogenicity, 1792

1977

('ylophaga Agarase Agar. 475 Cytophaga Agarase Broth. 475 Cytophaga agarovorans, 476.477. 1015 ('ytophaga agarovorans Agar, 475 Cytophaga agarovorans Broth, 476 Cytophaga allerginae, 1315 Cytophaga aprica, 1054 Cytophaga arvensicola, 643. 808 Cytophaga aurantiaca, 341. 477. 611.612. 1184 Cytophaga aurantica, 612 Cytophaga columnaris, 644 Cytophaga diffluens, 1054 Cytophaga fermentans. 476, 478. 808, 1015 Cytophaga fermenlans Medium, 476 Cytophagaflevensis, 257,258.260.288.475. 648, 1327, 1530 Cytophaga hutchinsonii, 341. 477. 609.611 Cytophaga hutchinsonii Agar, 476 Cytophaga hutchinsonii Broth. 477 Cytophaga johnsonacM'S, 1054.1575.1926 Cytophaga latercula, 477, 687, 808 Cytophaga fytica. 95, 1054. 1938 Cytophaga Marine Medium, 477 Cytophaga marinoflava, 95. 477. 1557 Cytophaga Medium. 477,478 Cytophaga Medium, Modified, 478 Cytophaga psychrophila, 643, 1051 Cytophaga saccharophila. 1893 Cytophaga salmonicolor. 1015. 1557 Cytophaga species, 135, 340, 349,472. 475, 478, 610. 612, 643. 709. 718. 741, 856, 890,1014,1015,1054.1237,1238,1350, 1539,1574,1594,1597, 1626, 1629, 1640, 1667. 1669. 1926 Cytophaga succinicans, 643 Cytophaga uliginosa. 808. 1558 Cytophaga xylanolytica, 717 Cytophaga xylanolytica DSM 6779. 1208 Cytosine Nutrient Agar, 478 CYU, 2%, 478 C/apek Agar, 479, 482 C/apek Agar with 20% Sucrose, 479 Czapek Agar with Sucrose, 479 C/apek Dox Agar, 480 Czapek Dox Agar with 20% Sucrose, 480 C/apek Dox Agar with 3% Glucose, 480 Czapek Dox Agar. Modified, 480 Czapek Dox Broth, 480 C/apek IX>x Liquid Medium, Modified. 481 C/apek Malt Agar. 481 C/apek Peptone Agar, 481 Czapek Peptone Yeast Agar. 481 Czapek Solution Agar, 481 Czapek Solution Agar with Sucrose. 481 Czapek Yeast Autolysate Agar, 482 Czapek Yeast Extract Agar, 482 D D/E HiVeg Agar Disinfectant Testing, 589, 590 D/E Neutralizing Agar, 486

1978

Index

DXB Neutralizing Broth, 486 D'L Neutralizing Broth Base, 486 DA Medium. 482 Daclylelia lysipaga, 1 004 Dactyleila nnnuta, 1004

Daclylelia oviparasilica, 1460 Dactyleila rhombos[X)ra. 1004 Daclylosparangium aurantiacum, 753, 1939 Dactylosporangium saimoneum. 753 Dactylosporangium species, 1318 Dactylosporangium lhailandense. 753 Dactylosporangium vinaceimi, 1319 Daedalea quercina, 999 Daiiy industry, 635, 636 Dairy products, 66. 212, 260, 261,262, 263, 274, 300, 302, 349. 645, 646.692. 954, 955,969, 1031,1032.1228. 1231, 1233. 1254,1403, 1404.1412, 1413. 1414, 1515,1516, 1517, 1559, 1560, 1631, 1644,1838. 1839. 1840. 1850. 1886 Damaged microorganisms, 585 Dap Nutrient Agar. 483 Daptobacter species, 934. 1168 Davis and Mingioli Glucose Minimal Medium. 483 Davis and Mingioli Medium A. 483 Davis and Mingioli Medium with Proline. 484 Davis and Mingioli Medium with Vitamin 1 -4, and Asparagine, 484 Davis and Mingioli Medium, Modified. 483. 484 Davis Supplemented Minimal Medium. 484 DCI.S Agar, 485 DCLS Agar. Hajna. 485 DCI.S HiVeg Agar. 485 DCLS HiVcg Agar. Hajna. 485 DCMYBA, 486 Debaryomxves hansenii, 589, 1942 Decarboxylase. 668 DecarlK>.\ylase Basal Medium, 486 Decarboxylase Basal Medium with Sodium Chloride, 486 Decarboxylase Base. Moller, 487 Decarboxylase Broth Base, Moeller, 1220 Decarboxylase Broth Base. Moeller with Arginine, 1220 Decarboxylase Broth Base. Moeller with Lysine, 1220 Decarboxylase Hi Veg Agar Base, 487 Decarboxylase HiVcg Broth Base. Moeller, 487 Decarboxylase Medium Base. Falkow, 487 Decarboxylase Medium, Ornithine Modified, 488 Decarboxylase Test HiVeg Medium Base (Falkow). 488 Decarboxylation, 487,488 Dcchloromonas agitata, 1045 Deep Liver Broth. 488 Deferribacler autolrophicus, 1693 Deferribacter desulfuricans. 489

Deferribacler Medium, 488 Deferribacter thermophilic, 489 Defined Glucose Medium KMSY-1,488.489 Defined Medium for Rhodopseudomonas, 489 IXlined Medium with Povidone Iodine, 489 Dehalobacler restriclus. 491 Dehalubacter restricttts Medium. 490, 491 Deinobacter grandis. 1455.1938 Deinococcus geothennalis, 1747 Deinococcus murrayi. 1747 Deinococais proleoiylicus, 454 Deinococcus radiodura/v:, 453,454, 1309 JJeinococcus radiophilus. 1428 Deinococcus species, 1472, 1705 Dekkera abstincus. 589.614. 1935 Dekkera anomala, 589. 614, 1934 Dekkera bruvellensis. 589, 614. 999,1934 Dekkera claussenii, 1934 Dekkem custersiana. 589. 614 Dekkera intermedia, 589, 614, 999 Dekkera lambica. 589. 614.1934 Dekkera naardenensis. 589, 614 Dekkera species. 607, 1038 IXlayed-incubation total coliform procedure, 1626 Deleytt aquamarina, 1343 Deleya halophila, 492,1343 Deleya halophila Medium, 492 Deleya salina, 1154 Deleya venusta, 1343 Delftia acidowrans, 1304, 1307 DcMan. Rogosa, Sharpc Agar. 1231 lXMan, Rogosa. Sharpe Broth, 1232,1233 Demetria terragena. 1513 IXmi-Fraser Broth, 492 Dendrophoma obscurans. 449 Dendnphtella arenarta, 1537 Dendryphiella salina, 1537 IXnitrification, 690,696, 1443 IXnilrifying bacteria. 154. 1189 Denitrovibrio acetiphilus, 493 Denitrovibrio Medium, 493 IXntal caries. 200, 1583

Dental plaque, 732.733,1485,1892 IXoxycholatc IXoxycholate IXoxycholatc IXoxycholate IXoxycholate IXoxycholate

Agar. 493, 494 Agar, HiVeg, 494 Citrate Agar. 494.495 Citrate Agar, HiVeg, 495 Citrate Agar. I lyncs. 495 Citrate lactose Sucrose Agar,

485 IXoxycholate Lactose Agar, 495 IXoxycholate Lactose HiVeg Agar, 496 IXoxycholate Lactose Sucrose Sorbitol Agar, 496 IXoxyribonuclcasc. 603, 604 IXoxyrilionuclease production, 1800 Dermabacter hominis. 496,11428.1470.932 Dennabacter Medium, 496 IXrmascl Agar Base, 496 Dermalocarjion Jluviatile, 951

Dermatophilus congolensis. 214, 215, 682 IXrmatophytc Test Medium, 496 IXrmatophytc Test Medium Agar. 609 IXrmatophyte Test Medium Base, 496 lXrmatophytes, 55, 586, 959.1396, 1527, 1528,1530.1531,1532 IXrmatophytic fungi, 496, 609, 713 lXrmatophytic fungi. 496 Dermocarpa species. 213. 214, 1200 Dennocarpella species, 1200 Dermocystidium Medium, 497 Dermoeystidium species. 497 Derxia gummosa. 167.497,827.828. 1296. 1297 Derxia gummosa Medium. 497 Derxia Medium. 497 Desemzia incerta, 455 lXsoxycholate Agar. 493 IXsoxycholatc Citrate Agar. 495 Desulfacinum hydrothermale, 498 Desulfacinum hydrothermale Medium, 497 Desulfacinum informal, 509 Desulfacinum Medium. 498 Desulfacinum species, 499 Desulfalirhabdium Medium,, 499 Desulfatirhabdium species. 500, 501, 503 Desulfitobacterium dehalogenans. 500. 501. 502 Desulfitobacterium dehalogenans Medium, 500 Desulfitobacterium hafniense, 501. 503 Desulfitobacterium hafniense Medium. 501 l~)esulfitobacteriwn Medium, 501 Desulfitobacterium PCE II Medium,, 504 Desulfitobacterium KB Medium, 502. 503 Desulfitobacterium species. 503. 504,505 Desulfoarculus species, 565 Desulfobacca acetoxidans, 506 Destdfobacca Medium. 505 Desulfobacter curvatus. 516 Desulfobacter giganteus, 553 Desulfobacter lulus, 516 Desulfobacter Medium. 506 Desulfobacter fXKstgatei, 507. 508 Desulfobacterpostgatei Medium, 507 Desulfobacter species, 506, 509, 1052 Desulfobacter species Medium. 508, 509 Desulfobacter vibrioformis. 509 Desulfohacterium aniline, 510 Desulfolxicterium anilini. 511,512 Desulfohacterium anilini Medium, 506. 509. 510,511 Desulfolxicterium Desulfohacterium Desulfohacterium 512 Desulfohacterium Desuljbbacterium Desulfohacterium Desulfohacterium 513

aulotrophicum. 515, 516 catecholicum, 512 catecholicum Medium, cetonicum, 513 cetonicum Medium, 512 indolicum. 514 indolicum Medium. 506.

Index

Desulfobacterium macestii. 562 Desulfohacterium Medium, 514 Desulfolxtcterium Medium with Lactate. 515 Desulfohacterium Medium, Modified, 516 Desulfobacterium oleovomns. 517 Desulfohacterium oleovomns Medium, 516 Desulfohacterium phcnolicutn, 518 Desulfohacterium phenolicum Medium, 517 Desulfobacterium species. 509. 570 Desulfohacula toluoltea, 519 Desulfobacula toluolica Medium. 518 Desulfobulbus etongafus. 520 Desulfohulhus Medium, 519, 520 Desulfobulbus proprionicus, 1409 Desulfobulbus species. 174, 509. 520. 521. 1052 Desulfobulbus spp. Medium, 521 Desulfocapsa suifexigens. 509 Desulfocapsa sulfoexigens, 522 Desulfocapsa sulfoexigens Medium, 522 Desulfocapsa thiozy-mogenes, 1662 Desulfocella halophila, 547 Desulfococcus amylolyticus- Medium, 522 Desuifococcus biacutus, 715,716 Desulfixoccus biacutus DSM 5651, 1207 Desulfococcus Medium, 523 Desulfococcus multiwrans. 524 Desulfococcus multivorans Medium, 524 Desulfococcus niacini, 524 Desulfococcus niacini Medium, 524, 525 Desulfofaba gelida DSM 12344.1625 Desidfofrigus fragile DSM 12345, 1613 Desulfofrigus oceanense DSM 12341. 1622 Desulfoglaeha Medium, 524 Desulfoglaeha species. 525 Desulfohalobium Medium, 525 Desulfohalobium utahense. 526 Desulfohalobium ulaheuse Medium., 526 Desulfohalobium ulaheuse Medium with Malate. 526 Desulfoluna Medium,, 527 Desulfoluna species. 527 Desuifomaculum nigrifictins, 562 Desulfomicrobium apsherotium. 562 Desulfomicmhium species, 528 Desulfomicrobium Willi Medium. 527 Desuifomonas pigra, 562 Desuifomonile Medium. 528 Desuifomonile tiedjei, 529. 530 Desuifomonile tiedjei Medium, 529 Desuifomusa hansenii. 531 Desulfomusa hansenii Medium. 530 Desulfonatronospira delicti, 533 Desulfonatronospira Medium,. 531, 532 Desulfonatronospira species, 531 Desulfbnatronovibrio hydrogenovorans, 533 Desuifonatronovibrio Medium. 533 Desuifonalronum lacustre, 534 Desulfonatronum Medium. 533 Desulfonema ishimotoi, 535 Desulfonema ishimotoi Medium. 534 Desulfonema limicola, 536, 537, 538

Desulfonema limicola Medium. 535. 536. 537 Desulfonema magnum. 539. 540 Desulfonema magnum Medium, 538, 539 Desulfonema species. 1052 Di'sutfontspora thiosulfaligenes, 1220 Desulfopila aestuarii, 1243 Desulforhahdus amnigena, 541 Desulforhahdus amnigenus, 541. 542 Desulforhahdus amnigenus Medium, 540, 541 Desulforhoptdtis singaporensis, 509 Desulforhopalus species, 509 Desulfosarcina Medium. 542 Desulfosarcina variabilis. 522. 542. 543 Desulfosarcina vartabdis Medium, 542 Desulfosporosinus orient is, 1451 Desulfotalea arctica DSM 12342, 1623 Desulfotaleapsyx.hrophila DSM 12343, 1624 Desulfothermus okinawensis, 1193. 1194 Desulfolignum halticum, 565 Desulfolobacterium species. 580 Desulfotomaculum acetoxidans, 544, 1052 Desulfotoinactdum acetoxidans Medium, 543, 544 Desulfotomaculum alkaliphilum, 545 Desulfotomaculum alkaliphilum Medium. 545 Desulfotomaculum geothennicum. 546 Desulfotomaculum geothennicum Medium, 545 Desulfotomaculum gihsoniae, 546 Desulfotomaculum CJroll Medium, 546 Desulfotomaculum halophiiumy 547 Desulfotomaculum halophilum Medium. 546, 547 Desulfotomaculum nigrificans, 174.762. 1051. 1409 Desulfotomaculum orientis, 1255, 1409 Desulfotomaculum rum in is, 1409 Desulfotomaculum sapomandens, 548 Desulfotomaculum sapomandens Medium, 547 Desulfotomaculum species, 174. 507, 548. 549, 562. 990, 1042. 1043. 1046, 1048, 1055,1638 Desulfotomaculum species Medium 11. 549 Desulfotomaculum spp. Medium I. 548 Desulfotomaculum thermobenzoicum. 1062 Desulfotomaculum thermosapovorans, 550 Desuljbtomaculum thermosapovorans Medium, 549 Dcsulfovenniculus halophilus. 527 Desuifovibiro desulfuricans, 562 Desulfovibrio aespoeensis. 551 Desulfovibrio aespoeensis Medium, 550 Desulfovibrio africanus. 175, 1409 Desulfovibrio alcoholovorans, 551 Desulfovibrio alcoholoxwvns Medium. 551 Desulfovibrio aspomum, 552 Desulfovibrio asponium Medium, 551 Desulfovibrio iMiarsii. 553, 1052. 1063

1979

Desulfovibrio haarsii Medium. 552 Desulfovibrio Brsickish Medium, 553, 829 Desulfovibrio carbinolicus. 554 Desulfovibrio carbinolicus Medium, 553 Desulfovibrio Choline Medium. 554 Desulfovibrio desulfuricans, 560, 562, 563, 1015. 1409 Desulfovibrio desulfuricans subsp. desulfuricans, 561 Desulfovibrio ferriredttcens. 1671 Desulfovibrio gabonensis, 555 Desulfovibrio galxfnensis Medium. 555 Desulfovibrio giganteus, 553, 556, 562 Desulfovibrio giganteus Medium. 555 Desulfovibrio gigas, 557, 1409 Desulfovibrio gigas Medium. 556 Desulfovibrio halophilus, 557, 558 Desulfovibrio halophilus Medium. 557 Desulfovibrio indonesiensis, 561 Desulfovibrio inopinatus, 559 Desulfovibrio inopinatus Medium, 558 Desulfovibrio magneticus, 559 Desulfovibrio magnelicus Medium, 559 Desulfovibrio Marine Medium, 560 Desulfovibrio Medium, 560, 561, 562 Desulfovibrio Medium with Lactate. 562 Desulfovibrio Medium with Sodium Chloride, 563 Desulfovibrio mexicanus DSM 13116. 824 Desulfovibrio MCi-1 Medium, 563 Desulfovibrio mullispirans, 1409 Desulfovibrio salexigens, 563, 1015 Desulfovibrio sapovorans. 26, 564. 1052, 1062. 1063 Desulfovibrio sapovorans Medium. 563 Desulfovibrio sax Medium, 564 Desulfovibrio senezii, 560 Desulfovibrio SI IV Medium, 565 Desulfovibrio sp. Medium, 566 Desulfovibrio species, 154,175, 555, 560, 562, 563, 566, 567, 569, 1042, 1043, 1046, 1048. 1051.1055.1196. 1409, 1638 Desulfovibrio sulfodismutans. 567. 568 Desulfovibrio sulfodismutans Medium, 567 Desulfovibrio vielnamensis, 560 Desulfovibrio vulgaris, 560, 562, 1015, 1409 Desulfovibrio vulgaris subsp. vulgaris. 561 Desulfovibrio zosterae. 569 Desulfovibrio zosterae Medium, 568 l}esuljbvigra adipica Medium, 569 Desulfovirga adipica, 570 Desulfurella acetivomns, 571 Desulfurella II Medium. 571, 572 Desulfurella kamchatkensis DSM 10409. 572 Desulfurella Medium, 570 Desulfurella multipotens. 573 Desulfurella multipotens Medium, 573 Desulfurella propionics DSM 10410. 573 Desulfurella species. 571 Desulfurococcus amylolyticus. 523 Desidfurococcus Medium, 573, 574

1980

Index

Desulfurococais mobilis. 574 Desidfurococcus mobility 574 Dcsulfurococcus mucosas, 574 Desulfurolohus ambivaletts, 49, 50 Desulfuromonas acetexigenes, 576, 579 Desulfuromonas acetexigenes Medium, 574, 575 Desulfuromonas acetexigens, 575 Desulfuromonas acetoxidans. 576 Desulfuromonas acetoxidans Medium, 576 Desulfuromonas Medium. 576. 577, 578 Desulfuromonas species, 577. 578 Desulfuromonas spp. Medium, 578 Desulfuromonas succinoxidans. 579 Desulfuromonas succinoxidans Medium. 579 Desulfuromonas thiophila. 575 Desulfuromusa hakii, 509, 711 Desulfuromusa ferrireducens. 1670 Desulfuromusa kysingii, 509, 71 ] Desulfuromusa succinoxidans. 509. 711 Desulsovibrio suljixlismutans Medium, 567 Desullobaclerium Medium. 580 Dethiohacter Medium, 580, 581 Dethiobacter species. 581 Dethiosidfovibrio II Medium, 582 Dethiosidfovibrio peptidovorans, 583 Dethiosidfovibrio peptidovorans Medium. 582.583 Dethiosidfovibrio species. 583 DFV Lactose Peptone MUG Broth, 583 Devosia riboflavina, 1306 Dexiostoma campyla, 1217 Dextran Agar, 584 Dextran Broth, 584 Dcxtran Medium,, 584 Dextran production, 1652 Dextrin fermentation, 60 Dextrin Fuchsin Sulfite Agar. 60 Dextrose Agar. 584. 585 Dextrose Ascitic Fluid Semisolid Agar, 585 Dextrose Broth. 585 Dextrose HiVeg Agar, 585 Dextrose HiVeg Agar Base, Hmmons, 586 Dextrose Hi Veg Agar with Blood, 586 DeXtlD9e I liVeg Broth, 586 Dextrose HiVeg Broth with Blood. 586 Dextrose I liVeg Peptone Agar, 586 Dextrose HiVeg Peptone Broth, 586 Dextrose Peptone Agar, 586 Dextrose l*roteose No. 3 Agar. 587 Dextrose Proteose Peptone Agar Base with Tellurite and Blood. 587 Dextrose Proteose Peptone HiVeg Agar Base with Tellurite and Blood. 587 Dextrose Soil Agar. 587 Dextrose Starch Agar, 587 Dextrose Sucrose Cellulose Agar. 588 Dextrose Tryptone Agar, 588 Dextrose Tryptone Broth. 588 Dextrose Tryptone HiVeg Agar, 588 Dextrose Tryptone HiVeg Agar. Modified, 589

Dextrose Tryptone I liVeg Broth. 589 Dextrose Tryptone HiVeg Broth, Modified, 589 Dextrose Yeast Asparagine Agar, 589 Dextrose Yeast Hxtract Peptone. 589 IXy/Fngley Neutralizing Broth, 486 IXy/Fngley Neutralizing Broth Base, 486 IXy-F.ngley Neutralizing Agar, 589 Dcy-Hnglcy Neutralizing Broth Base, 590 Dey-Engtey Neutralizing I liVeg Agar, 590 Dcy-Knglcy Neutralizing HiVeg Broth, 590 Dey-Engley Neutralizing HiVeg Broth Base, 590 DFI Agar. 258 IXi 18 Agar, 593 Diagnostic bacteriology. 1775 Diagnostic Sensitivity Test Agar, 590 Diagnostic test, 70 Dialister fulvus, 591 Dialister Medium. 591 Dialislerpneumosintes, 371 Dialister species. 591 Dialister vulgaris, 591 Diamalt Agar, 591 Diaminopimelic Acid Medium, 591 Diamonds Medium. Modified. 592 Diaphanocca grandis. 1015 Dia/otrophic Medium, 592 Dibenzothiophene Mineral Medium, 592 Dichloran. 18% Glycerol Agar. 593 Dichloran Glycerol Agar, 593 Dichloran HiVeg Medium Base with Rose Bengal and Selective Supplement. 593 Dichloran Medium Base with Rose Bengal and Selective Supplement. 593 Dichloran Rose Bengal Chloramphenicol Agar. 594.607 Dichloroacetic Acid Medium No. 1. 594 Dichloroacetic Acid Medium No. 2, 594 Dichloromethane Medium for llyphomicrohium, 594 Dichloromethane utilizing bacteria, 1176 Dichlorophenoxyacetic acid utilization, 1176 Dichotomicrobium thermohalophilum, 595 Dichoiomicrobium thermohalophilum Agar, 594 Dichoiomicrobium thermohalophilum Broth. 595 Dichotomomyces cejpii, 706 Dicranidion fragile, 944 Dicranidion gracilis. 944 Dictoglomus ihermophilum, 596 Dictyochloris fragmns. 70 Diclyoglomus Medium, 596 Dictyoglomus tutgidus. 110. I l l Diclyophora indusiata, 684 Dictyophora plialloidea. 684 Diclyosphaerium ehrenbergianum, 70 Dictyosphaerium pulchellum. 70 Dictyostelium discoideum. 5% Dictyosteiium Medium, 596 Dictyostelium species. 930

Didymium nigripes. 930

Dientamoeba fragdis, 1859 Diethyl Phosphonate Agar. 596 DI1T7BCYF,281 Differential Agar for Group D Streptococci, 596 Differential Agar Medium A8 for UreaplasIIHI urealyticum. 597 Differential Broth for I^actic Streptococci, 597 Differential Buffered Charcoal Yeast Extract Agar Base with Selective Supplement, 597 Differential Components. 7 Differential Reinforced Clostridial HiVeg Broth Base with Ferric Citrate and Sodium Sulfite, 598 Diffusion disk testing, 1937 Diheferospora chlamydosporia. 952

Dihydrolase Broth Base with Arginine, 598 Dihydrolasc HiVeg Broth Base with Arginine, 598 Diluent. 1026 Dilute Peptone Water, 598 Dilute Potato Medium, 599 Dimasligeila species, 161, 1589 Dimastigella trypaniformis. 161 Dimorphic pathogenic fungi, 1931 Dinollagellate Medium, 599 Diphasic Blood Agar Base Medium. 599 Diphasic Blood Agar Medium with 10% Blood, 600 Diphasic Blood Agar Medium with 30% Blood. 600 Diphasic Blood Culture Buffered Charcoal Yeast Fxtract Medium. 942 Diphasic Medium for Amoeba, 600 Diphosphothiaminc Medium. 601 Diphtheria, 1566 Diphtheria Virulence HiVeg Agar Base with Tellurite and Diphtheria Virulence Supplement, 601 Diplonema species, 644 Diplophyrs marina. 1825 Dipsacomyces acuminosporus, 809 Direct plating, 156.290,291,649.650,1069. 1242. 1327 Disarticulatus devroeyi, 706 Disarticulatus indicus. 706

Disc plate technique, 1408 Disciotis wnosa, 1002 Disinfectant products, 135 Disinfectant Test Broth. 601 Disinfectant Test Broth AOAC, 602 Disinfectant Test HiVeg Broth. 602 Disinfectant Test Medium. 602 Disinfectants. 486, 590, 602.670, 1671 Disk method, 465 Distribution systems, 1427 Dithionite Thioglycolate, HS T, Broth, 391 Dilrichomonas honigbergii, 1859 Dixon Agar, 602

Index

DM Medium, 602 DMA Medium, 602 DNase Agar, 603 DNase Medium, 603 DNase production, 1800 DNase Test Agar, 603 DNase Test Agar with Methyl Green, 603 DNase Test Agar with Toluidine Blue, 604 DNase Test I liVcg Agar Base. 604 DNase Test HiVeg Agar Base without DNA, 604 DNase lest HiVeg Agar with Toluidine Blue. 604 DNB Medium. 604 Doejiel Medium, 604 Dorset Egg Medium. 605 Double Sugar Agar, Russell, 605 Double Sugar HiVcg Agar, 606 Double-layer agar technique, 1636 Double-Strength Crude lMctobacillus Medium, 605 IXwble-Strcngth Crude Medium for Lactobacillus, 605 Doyle and Roman Enrichment Medium, 606 Doyle's Enrichment Broth Base with Antibiotic Solution, 606 Doyle's Enrichment 1 liVcg Broth Base with Antibiotic Solution, 606 DP Agar. 607 DPA with Calcium Carbonate, 607 DPM Medium, 607 DRBC Agar. 594,607 Drechslera bicolor, 1410 Drechslera biseplata, 614. 930 Drechslera catenaria, 928, 1877 Drechslera cynodonlis, 1410 Drechslera graminea, 1410 Drechslera panicimiliacei. 1877 Drechslera rostrala, 1410 Drechslera sorokiniana, 1410 Drechslera spicifera, 1410 Drechslera teres, 1410 Drechslera verticillata, 1410 Drechslera victoriae. 1410 Dried foods, 978, 1032 Drigalski Litmus Lactose Agar. 607,608 Dngalski Litmus Lactose Agai, Modified, 608 Drigalski Litmus Lactose HiVeg Agar, 608 Drasophila, 1551 Druggan, Forsythe and Iverson Agar, 258 Drug-resistant bacteria, 1164, 1165, 1469 DS HiVeg Medium. 1203 DS Medium, 1203 DS Sporulation Medium. Modified, 613 DSA, 587 DSA Cellulose, 588 DSIC Medium, Modified, 608 DSM134.776 DOT Agar, 590 DTC Agar. 609 DIM Agar. 609

Dubos Agar with filter Paper. 609 Dubos Broth, 609 Dubos Broth Base with Serum and Glycerol. 610 Dubos Broth with Horse Scrum, 610 Dubos HiVeg Broth Base with Serum and Glycerol. 610 Dubos Medium Albumin. 4 Dubos Mineral Medium, 610 Dubos Oleic Agar, 610 Dubos Oleic Albumin Complex, 4 Dubos Oleic HiVeg Agar Base with Serum and Glycerol, 611 Dubos Oleic HiVeg Broth Base with Antibiotic and Oleic Albumin Supplement, 611 Dubos Salts Agar. 611 Dubos Salts Agar with 1% Sodium Chloride, 611 Dubos Salts Agar wiUi Yeast Extract, 612 Dubos Salts Broth, 612 Dubos Salts Broth with Yeast Lxtract, 612 Dubos Salts Medium,, 612 Ducreyi Medium, Revised. 772 Dulaney Slants, 612 Dulcitol Selenite Broth. 613 Dunaliella bardawil, 483 Dunaliella tertiolecta, 614 Duncan-Strong Sporulation Medium, Modified, 613 Dung lixtract Agar, 613 Dunkelberg Agar, 1365 Dunkelberg Carbohydrate Medium. Modified, 613 Dunkelberg Maintenance Medium. 613 Dunkelberg Semisolid Carbohydrate Fermentation Medium. 614 DV Median, 614 DYA with Calcium Carbonate, 614 DYAA. 589 DYA A Cellulose, 614 DYAA Cellulose Malt. 614 DYAA LUP, 615 DYPA. 589 E E Agar, 615 E Medium for Anaerobes. 615 E Medium for Anaerobes with 0.1% Cellobiosc. 616 E Medium for Anaerobes with 0.2% Rutin. 618 h Medium for Anaerobes with 0.3% Phloro-

glucmol, 617 K Medium for Anaerobes with Filtered Rumen Fluid and 0.1% Cellobiose, 616 E Medium for Anaerobes. Modified. 617 /â&#x20AC;˘:. colt, 257, 262, 264,386, 437, 584, 623, 696.836.844.932.% 1.991.1404.1686. 1837,1894 E. coli 0157:117,200,615,622, 695, 840. 1320,1478 E. coli 0157:117 Agar, Fluorocult, 695

1981

E. coli 0157:117 MUG Agar, 615 E.T. Medium, 656 E.V.A. HiVeg Broth. 657 Eagle Medium. 618.619.620 Eagle Medium. Modified, 620 Eagle's Minimal Essential Medium with Earlc's Salts and Nonessential Amino Acids, 621, 654 Earlc's Balanced Salts. Phenol Red-Free. 621 KB Motility Medium. 621 EBA Medium, 621 EC Broth, 622 EC Broth with MUG. 622 EC Medium, Modified with Novobiocin, 623 ECD Agar. 623 KCD Agar, Fluorocult*, 623 ECD MUG Agar, 623 Echinamoeha Agar, 624 Echinamoeba Broth. 624 Echinamoeha exundans, 1454 Echinamoeba species. 624 Echinosleliinn minutuin, 759 Ecithinasc production. 1034 ECM Agar, 624 Ectothiorhodospira abdelmalekii, 625, 628 Ectothiorhodospira abtielmalekii Medium. 624 Ectoihiorhodospira halochloris. 625, 627. 628, 1049 Ectothiorhodospira halochloris Medium, 625 Ectoihiorhodospira haiophila. 626, 627 Ectothiorhoilospira haiophila Medium. 625. 626 Ectothiorho<lospira marisinortui, 381. 382, 1507 Ectothiorhodospira Medium. 627 Ectothiorhodospira Medium. Modified, 628 Ectothiorhodospira mobilis, 381. 382. 1377 Ectothiorhoilospira shaposhnikovii. 1080 Ectoihiorhodospira mculota, 629 Ectothiorhodospira vacttolata, 628, 629 Ectothiorhodospira vacuolata Medium. 628 Ectothiorhoilosynus Medium. 629 Ectothiorhodosynus species, 629 Etiaphobacter aggregans, 811 Edaphobacter modestus. 810 Edelstein BMPA-a Medium, 234 EDTA utilization, 1049 Edwards and Bruner Semisolid Medium. 630 Edwards and Ewing HiVeg Medium, 1225 Edwards Medium HiVeg Base. Modified. 630 Edwards Medium, Modified. 630 BE Broth, 630 EE Broth. HiVeg, 630 EE Broth, Mossel, 631 EE HiVeg Broth. Modified. 631 EE HiVeg Broth, Mossel, 631 Eeniella nana, 749 EG Agar, 631

1982

Index

EG Medium, 631 EG Sodium Chloride Medium No. 7, 631 Egg Meat Medium, 632 Egg products, 212. 261, 1541 Egg Tellurite Glycine l*yruvatc Agar, 656 Egg Yolk Agar. 632 Egg Yolk Agar Base. HiVeg with ligg Yolk Emulsion, 632 ligg Yolk Agar with Neomycin, 965 Egg Yolk Agar, I.omlxird-Dowell, 964 Egg Yolk Agar, Modified, 632 Egg Yolk Emulsion, 4, 633 ligg Yolk Emulsion, 50%, 4,633 EGGC.633 Eggs. 212,1541 E1IHC serovar BCM ()157:117(+), 200 EIA Substrate, 634 Eijkman lactose HiVeg Broth, 634 Eijkman I <ictosc Medium, 634 Eikcnclla eorrodens, 721, 1458 Ekho I.ake Strains Medium, 634 Elakalolhrix viridis, 70 Elek Agar, 901 Eleulherascus lectardii, 706 Elicobacler species, 1249 Ellikcr Agar. 635 Elliker Broth. 635.636 Ellikcr Broth. HiVcg. 920 Elliker HiVeg Broth, 636 Elliker I-aclose Broth, 636 EMB Agar, 636 EMB Agar Base. 637 EMB Agar. Modified, 637 EMB HiVeg Agar. 637 EMB HiVeg Agar, I .evine, 637 Emheilisia allii, 1410 Emheilisia chlamydospora, 1877 KmericeUa nidulans, 155 Emericellojxsis minima, 761 Emerson Agar. 637 Emerson Agar, Half Strength, 638 Emerson HiVeg Agar. 638 Emerson Yp Ss Agar. 638 Emerson Yp Ss Agar with 25% Seawater and a Birch Stick, 638 Emerson YpSs Agar with 0.25% Seawater. 638 Emerson YpSs Broth, 1/2 Strength, 638 Emerson's Yeast Starch Agar, 639 Emmon's Modification of Sabouraud's Agar, 639 Emmon's Modification of Sabouraud's Agar with 0.5% Yeast Extract in Olive Oil, 639 ENB Agar. 639 Encepiuilitozoon funiculi. 1190 Encepludttozuvn he] fern. 1190 Encepiuilitozoon inleslinalis, 1190 Endo Agar, 640 Endo Agar Base, 640 Endo Agar with Sodium Chloride, 641

Endo Agar, l.aurance Experimental Station. 641 Endo Agar, I.ES, 641 Endo Agai, Modified, 641 Endo Broth. 642 Endo HiVeg Agar, 642, 644 Endo HiVeg Agar Base, 642 EndO HiVeg Agar with NaCI, 642 Endo HiVeg Agar. Modified, 642 Endolimav nana, 226 Endospore formation, 1774 Endospores, 117 Endothia Complete Agar, 643 Endothia Complete Broth, 643 Endotrypanum species. 600 Enhydrobacter aerosaccus, 320 Enhygromyxa salina, 1899 Enriched Cytophaga Agar, 643 Enriched Cytophaga Agar Medium,, 643 Enriched Cytophaga Medium. 644 Enriched banana Medium, 644 Enriched Nutrient Agar, 644 Enriched Nutrient Broth, 644 Enriched Thioglycollatc Hi Vcg Broth. 644 Enrichment Broth tor Aeromonas hvdrophila. 645 Enrichment Broth, pi I 7.3. 645 Enrichment Broth, pll 7.3 widi Pyruvate. 645,646 Ensifer adhaerens. 688 Entamoeba barreti, 1861 Entamoeba coli, 1859 Entamoeba dispar. 647, 1859 Entamoeba dispar Axenic Culture Medium, 646 Entamoeba gingival is, 1859 Entamoeba histolytica. 195,647.1349,1859. 1861 Entamoeba Hi Vcg Medium. 647 Entamoeba insolita, 1859, 1861 Entamoeba invadens, 1861 Entamoeba Medium, 647 Entamoeba moshkovskii, 1859, 1861 Entamoeba polecki. 1859 Entamoeba rananim, 1859, 1861 Entamoeba species, 1946 Entamoeba terrapinae, 1861 Entercocci, 290.291, 649.650.655. 1069, 1242 Enteric bacilli, 201. 206.223,224,446. 605. 1030,1537,1572,1625 Enteric bacteria. 993. 1695 Enteric fermentation Base, 647 Enteric microorganisms, 493, 494 Enteric pathogens, 374, 375. 494,495. 496, 990. 991,992,993,1253, 1918,1919, 1920 Enterobacteaceae, 1847 Enterobacter aerogenes, 907. 1330 Enterobacler agglomerans, 977 Enterobacter cloacae, 255. 750,934,935. 1382

Enterobacter Medium, 648 Enterobacter sakazakii, 146, 384, 838 Enterobacter species, 218. 261, 493.494, 648, 654 845 Enterobacleria Enrichment Broth, Mossel, 648 Enterobacteriaceae, 118, 119, 218,266, 312, 313,374, 375,455, 742,843, 889,893. 903.904, 905.906,928.930.976. 977, 991,1167,1223,1225,1226,1330,1448, 1449.1478,1534, 1572.1819.1820, 1869. 1870, 1871, 1872, 1886, 1887 Enterobacteriaceae Enrichment Broth. 630 Enterobacteriaceae Enrichment Brotli, Mossel, 631 Entcrobateriaccac, 630.631 Entcrococci. 163.218. 389.615.648.649. 655.657. 658. 762.838.843.890. 891, 897,991, 992,1068, 1140, 1481, 1567, 1577,1584.1702. 1895 Entcrococci Broth, Chromocult, 648 Entcrococci Confirmatory Agar. 648 Enterococci Confirmatory Broth, 648 Entcrococci HiCrome™ Broth, 838 Enterococci Presumptive Brotli. 649 Enterococci Rapid HiCrome™ Agar, 843 Enterococcosel™ Agar, 649 Enterococcoscl™ Broth. 649 Enierococcus Agar. 649 Enierococcus avium, 679, 680, 1428 Enierococcus casselijlavus, 454. 1428 Enterococcus cecorum, 441. 1428 Enierococcus columbae, 441 Enierococcus Confirmatory Hi Vcg Agar with Penicillin, 649 Enierococcus Confirmatory HiVeg Broth with Penicillin. 650 Enierococcus dispar, 1428, 1945 Enterococcus durans. 56. 744, 1428 Enierococcus faecalis, 454, 679,680, 744, 751. 1410, 1429.1643.1802, 1933 Enierococcus J'aecium, 141, 454, 650, 679, 680.744.816.839, 1429 Enterococcus J'aecium Medium, 650 Enterococcus gallinarum. 1429 Enterococcus hirae, 215,454,697,699,700, 744 Enterococcus hirae AICC 8043, 91 Enierococcus malodomtiK, 1429 Enterococcus mundtii. 1429. 1933 Enterococcus parauberis, 441 Enterococcus Presumptive I liVeg Broth with Penicillin, 650 Enterococcus pseudoavhan, 441,1429.1933 Enterococcus raffinosus, 441, 1429, 1933 Enterococcus saccharolyticus. 441, 1429 Enterococcus serialicida, 1429 Enterococcus solilarius. 1429, 1933 Enierococcus species, 260, 377, 378, 386, 679. 680. 984. 1381. 1449. 1479. 1704. 1803. 1829

Index

Enterococcus sulfureus, 1429. 1933 Entemcolitica agglomerans, 1350 Enteropathogcnic Escherichia coli. 1589, 1590 Enterotoxigenic Escherichia coli, 321. 439 Enterotoxigenic staphylococci, 1242, 1633, 1634 Enterotoxin production, 245 Enterov indent Escherichia coli, 1248

Enteroviruses, 914 Entomophthora aphidis. 1460 Entomophthora ignobilis, 1460 Entomophthora sphaerospenna, 1460 Entosiphon species. 1015 Environmental, 201,202,203,204,205,281, 298.299.332.704.705.775.1156.1157. 1202, 1217,1227, 1243,1244, 1245, 1246.1256,1482.1517 Environmental waters, 758 Eosin Methylene Nine Agar, 636 Eosin Methylene Blue Agar. I.evine, 950 Eosin Methylene Blue Agar, Modified, 637 Epidermopiiyton JJoccosum, 706, 1532 Epoxysuccinic Acid Medium, 653 Eremascus albus. 809.988, 1332 Eremascns fertilis, 809,988, 1332 Eriopeziza caesia. 1004 Erratia marce.scens, 603 Erwina uredovora. 935 Eivinia amylovora, 651, 1318, 1368, 1830 Erwinia amylovora Selective Medium, 650 Erwinia chrysanthemi, 751 Erwinia Fermentation Medium, 651 Eminia herbicola, 749. 1635 Erwinia mallotivora. 1368 Erwinia Medium 1)3, 651 Erwinia nigrifluens. 1368 Erwinia salicis, 1368 Eminia Selective Medium. 651 Erwinia species, 464,651, 748,1563,1929, 1943 Erwinia tracheiphila, 652. 1916 Erwinia tracheiphila Agar, 651 Erwinia uredovora. 934 Erynia blunckii, 1460 Erysipelothrix Medium, 652 Eiysipelothrix rhusiopathiae, 162, 306, 463 Erysipelothrix species. 652 Eiysipelothrix fonsillarnm. 1850 Eiythritol Agar. 652 Erythritol Broth, 652 Erythrohacter litoralis. 652 Erythrobacter longus, 652, 1009. 1050, 1420 Eiylhrohacter longus Medium. 652 Eiythromicrobiuin ramosmn. 652 Eiythroinicrobiinn roseococcus Medium, " 652 Erythromycin I, Broth Medium, 652 Erythromycin EB Medium, 653 ESA Medium, 653 Escherichia coli, 11,16, 22,94, 121. 131, 171, 172, 215, 258, 260, 261, 263,321,

323, 336. 348, 352. 353, 377. 384,439, 445,478,483,484,485,493,494,607, 622.613, 624. 634.636.637.639.644. 653, 654. 750, 751, 769, 771. 807, 815. 821, 833, 834, 835,836,837,838, 841, 842. 843. 844. 851.875, 878,891. 892. 893, 907, 911, 912, 913,914,931, 932, 933.934.935.936.937.938.939.950. 973, 981, 982, 983, 988. 989,994, 1039. 1040.1066. 1168. 1187. 1189.1190. 1196.1252. 1253. 1254.1283.1308. 1309, 1310, 1311, 1313, 1319, 1330. 1364, 1437, 1438. 1478. 1480.1481, 1514, 1536, 1537. 1571, 1589. 1590. 1625, 1627, 1647, 1666, 1671, 1683, 1690. 1696. 1697. 1698, 1799. 1803. 1821,1822, 1823. 1836, 1837.1838. 1839, 1840, 1843, 1844, 1845, 1854, 1857,1858. 1859. 1860, 1886. 1889. 1890, 1923, 1934, 1940, 1952 Escherichia coli Broth. 622 Escherichia coli enterotoxin. 1923 Escherichia coli JM strains. 1178 Escherichia coli NRRE B-, 766, 1645 Escherichia coli 0157:117. 334. 623. 835. 838, 1218. 1252. 1320, 1593 Escherichia coli plasmids, 1697 Escherichia coli tryptophan auxotroplis. 984 Escherichia Medium, 653, 654 Bsculin Agar. 654 Esculin Agar. Eomhard-IXwell, 964 Bsculin Agar. Modified CDC. 654 Esculin A/ide Broth, 655 Esculin A/ide I liVeg Broth, 655 EflCUlin Broth, 655 Esculin hydrolysis, 189,190

Esculin hydrolysis, 218,705,706,939,964 Esculin Iron Agar, 655 Esculin Mannitol Agar, 655 Bsculin Thallium Medium, 655 ETGPA, 656 Ethanoligenes Medium.. 656 Ethanoligenes species. 656 EUvyl Violet Azide Broth, 657 Ethyl Violet A/ide HiVeg Broth, 657 Ethylene Glycol NaCl Medium No. 7, 631 Ethylene Production Agar for Mucor. 658 Ethylene Production Broth for Mucor. 658 ETSA Medium, 658 Eubacteiium acidaminophilum, 622, 660 Eubacterium acidaminophilum Medium, 659 Eubacteiium aggregans, 660 Eubacteiium aggregaivt Medium. 660 Eubacterium alactolyticum. 88, 718 Eubacterium august urn, 661 Eubacterium angustum Medium, 660 Eubacterium barkeri, 1289 Eubacterium biforme. 373, 374 Eubacterium budayl, 88 Eubacteiium cxillanderi. 661. 677 Eubacteiium callanderi Medium, 661 Eubacterium cellulosolvens, 616, 1419

1983

Eubacterium combesii, 1459, 1549 Eubacteiium contortum, 1549 Eubacteiium coprostanoligenes. 198 Eubacteiium desmolans, 1452 Eubacteiium form icigenerans. 3 70 Eubacterium hallii, 373 Eubacterium lentum. 372, 662 Eubacteiium lentum Medium, 661 Eubacteiium limosum, 254. 366. 1459 Eubacteiium Medium, 662 Eubacteiium moniliforme. 88 Eubacteiium multiforme, 1459 Eubacteiium nitritogenes, 1459, 1461 Eubacterium oxidoreducens. 663. 665 Eubacterium oxidoreducens Medium. 665 Eubacteiium plant it, 366 Eubacteiium ivminantium, 366, 616, 617, 1419 Eubacteiium saburreum, 366 Eubacteiium siraeum, 366 Eubacterium species, 56, 57, 366, 367. 369, 371.374.662 Eubacteiium tarantellus, 366 Eubacteiium tenue, 366, 1459 Eubacterium tortuosum, 88 Eubacteiium ventriosum. 366 Eubacteiium xylanophilum. 282 Euglena B ( 2 Medium, 663 Euglena gracilis, 860 Eugon Agar, 663 Eugon Agar with Fildcs Enrichment. 663 Eugon Blood Agar, 664 Eugon Broth. 664 Eugonagarâ&#x201E;˘, 664 EugonbrouVM. 664 Eugonic Agar, 664 Eugonic HiVeg Agar, 664 Eugenic HiVeg Broth, 665 Eupenicillium brefeldianum, 944 Eupenicillium molle. 1935 Euplotes aediculatus, 1589 Euplotes harpa, 1556 Europhium clavigerum, 1004 Eurotium chevaiieri, 809. 1332 Eurotium glabrum. 1332 Eurotium halophilicum, 809. 988 Eurotium herbariorum, 809, 988 Eurotium intermedium, 1332 Eurotium repens. 480 Eurotium ruhrum, 1332 Eurotium sjK'cies, 479 Eurotium tonophilum, 1332 EVA Broth, 657 Excellospora species, 159, 320,444,907. 1153, 1318.1877, 1927 Excellospora \iridilutea, 210,1322 Exiguobacterium aurantiacum. 665, 1425 I'xigunbaclerium Medium, 665 Extracted Hay Medium, 665 EY Tellurite Enrichment, 4 EYGA Agar. 665 FYS Agar, 639

1984

Index

F F.G. HiVcg Agar. 671 F.G. HiVcg Broth, 671 FAA, 666 FAA Alternative Selective. 666 FAA Alternative Selective with Neomycin, Vancomycin, and Josamycin, 666 FAA Selective, 667 FAA Selective with Neomycin and Vancomycin, 667 Fabrics. 16 Facultative, 732 Falcivibrio grandis. 659,666, 1890, 1905 Falcivibrio Medium. 665 FalcMbrio vaginalis, 659, 666, 1890, 1905 Farlowiella cannichaeliana. 999 Fastidious Anaerobe Agar. 666 Fastidious Anaerobe Agar, Alternative Selective, 666 Fastidious Anaerobe Agar, Alternative Selective with Neomycin, Vancomycin, and Josamycin, 666 Fastidious Anaerobe Agar. Selective. 667 Fastidious Anaerobe Agar, Selective with Neomycin and Vancomycin, 667 Fastidious bacteria, 231, 324, 325,1309, 1593 Fastidious fungi, 248, 249, 253 Fastidious microorganisms. 254, 358. 1848, 1881 Fawlus arcularius. 392 Fay and Barry Medium. 668 Faybitch's Sucrose Gelatin Agar, 668 IB Medium, 668 FC Agar. 669 FC Broth. 669 FC1C, 670 FDA Agar. 670 FDA Broth, 670 Fc(III) Lactate Nutrient Agar. 670 Fecal. 657,658 Fecal, 162,163.191,192,201,266,267,271, 290, 291,292, 298,299, 309,398,418, 649,650.669.670.896.897,898, 1026. 1069, 1150, 11%, 1242, 1809, 1813 Fecal Col i form Agar. 669 Fecal Coliform Agar, Modified, 670,671 Fecal Coliform Broth, 669 Fecal coliform microorganisms. 671 Fecal coli forms, 11, 669, 670, 1150 Fecal enterococci, 657,658 Fecal specimens, 201. 267, 291, 292, 298. 299,309,398,418,485,486,1222,1247, 1538,1699, 1700,1701,1943 Fecal streptococci, 162,163,191, 192,201, 266. 290, 291. 649,650. 896,897.898, 1026,1069, 1242 Fecal transport, 1598 Fecal treponemes, 1809. 1813 Feces, 141,260, 262, 263. 290, 291. 470, 649, 650, 655, 692,1069, 1242, 1303,

1314,1327,1515,1516. 1517,1559, 1560,1700, 1701 Feed. 212 Feed materials, 952, 976 Feeds, 978.1032. 1258 Fecley-Gorman Agar, 678 Feeley-Gorman Agar with Selenium, 678 Feeley-Gorman Broth, 678 Feeley-Gorman HiVcg Agar. 671 Feeley-Gorman HiVeg Broth, 671 Fomentation reactions. 1385. 1386 Feodorov Medium, 671 Fermentation Basal Medium. 671

Fermentation base, 1774 Fermentation Base for Campylobacter, 647 Fermentation Broth, 672 Fermentation HiVeg Medium Base for C. perfringens with Salicin and Raffmose. 672 Fermentation HiVcg Medium for Ncisscriac with Carbohydrate, 672 Fermentation HiVeg Medium for Staphylococcus and Micrococcus, 673 Fermentation Medium, 673 Fermentation Medium Base for C. perfringens with Salicin and Raffmose, 673 Fermentation Medium for Ncisscriac with Carbohydrate, 673 Fermentation Medium for Staphylococcus and Micrococcus. 674 Fermentation reactions, 1385, 1386,1388 Fermentation studies, 358

Fermentation tests. 466 Fermentative, 821 Ferric Citrate Medium, 674 Ferriplasma acidophilum. 675 Ferroglobuspiacidus Medium, 674 Fetroglobus piacidus. 675 Ferroplasma acidiphilum, 675 Ferroplasma acidiphilum Medium, 675 Ferrous Sulfated'east Fxtract Medium,, 675 Ferrous Sulfide Agar. 676 Ferulale Medium, 676 Fen'idobacterium gondwanense. 1704 Fen'idobacterium islandicum, 677, 678 Fen'idobacterium islandicum Medium, 677 Fen'idobacterium Medium, 677 Fen'idobacterium nodosum, 198.678 Fen'idobacterium penniwrans. 1704 Fen'idobacterium species. 1704 F-G Agar, 678 F-G Agar with Selenium, 678 F-G Broth, 678 FGTC Agar, 679 FGTC Agar Base with Bicarbonate, Gcntamicin and Amylose Azure, 679 FGTC HiVeg Agar Base with Bicarbonate, (icntamicin, and Amylose Azure, 679 Fibrobacter intestinalis, 681 Fd)robacter inyrdyinslid, 616 Fibrobacter specks. 617 Fibrobacler succinogenes, 681, 1419

Filamentous fungi, 469. 482. 1000. 1001, 1002 1'ildes Hnrichmcnt. 4

Fildes Enrichment Agar, 681 Filobacilius milosensis, 681 Filobacillus milosensis Medium, 681 Filobasidiella depauperata, 1878 Filobasidiella neofonnans. 749, 1026 Filobasidiumflorifonnc. 974. 1476 Filomicrohium fusiforme, 243,987 Filtration. 9 FischereUa species, 213, 214, 233, 234, 326 Fish, 800, 802, 880, 1554 Fish Peptone Agar. 681 Fish Peptone Broth, 682 Five g Agar. 682 Flahelfula hoguae, 243 Flagella, 682 Flagella Broth, 682 Flammeovirga aprica. 477 Flammulina velutipes, 775 Flat-sour sporeformcrs. 762. 1569 Flat-sour bacteria, 588, 589 Flavimonas oryzihabilans, 1304 Flavobacterium acidurans, 683 Flavobacterium aquatile, 643.682.683 Flavobacterium atptalile Medium, 682 Flavobacterium branchiophila, 643 Flavobacterium branchiophilum, 478 Flavobacterium brew, 1304 Flavobacteriumfilamentosum.4 59 Flavobacterium glacie, 1458 Flavobacterium gondwanense. 1009 Flavobacterium indologenes, 1928 Flavobacterium indolihelicum. 682 Flavobacterium leparinum, 821 Flavobacterium lucecoloratum, 1001 Flavobacterium lutescens, 683 Flavobacterium M1 Agar, 682 Flavobacterium Medium, 682, 683 Flavobacterium Medium M1.683 Flavobacterium meningosepticum. 983 Flavobacterium mizutaii, 1304 Flavobacterium omnivorum. 1458 Flavobacterium resinovonnn Agar, 683 Flavobacterium salegens, 1009 Flavobacterium species, 117,683, 684, 800, 802. 860. 1003. 1218. 1361.1406. 1552.

1555. 1814,1822, 1847 Flavobacterium tirreniculum, 1308 Flavobacterium tirrenicum, 799 Flavobacterium tirrenicum Medium. 683 Flavobacterium uliginosum. 799 Flectobacillus major. 116. 1051. 1158, 1218 Fiectobaciilus marinus. 1554 Flegler's Minimis Medium, 684 Fletcher Leptospira HiVcg Medium Base. 684 Fletcher Medium. 684 Fletcher Medium with Fluorouracil, 684 Fletcher's Semisolid Medium. 684 Flexihacler Agar, 684, 685

Index

Flexibacter uggregans. 1054 Flexibacterauranliacus. 643,687. 1051. 1054 Flexibacter Broth. 685 Flexibacter canadensis, 643. 686 Flexibacter canadensis Agar. 686 Flexibacter canadensis Medium. 686 Flexibacter colwnnaris. 96.741, 808 Flexibacter elegans, 685, 688, 1051 Flexibacterfiliformis, 257, 258, 260, 288, 648, 1327, 1530 FlexibacterJlexilis. 687. 1051 Flexibacter litorale. 687 Flexibacter litoraiis, 477. 1054 Flexibacter marinum. 687 Flexibacter maritimns, 687. 1015. 1688 Flexibacter Medium, 686. 687 Flexibacter polymorphic. 685, 686. 687 Flexibacter polymorphic Medium. 687 Flexibacter psychrophilus. 96 Flexibacter roseolus, 687, 1051 Flexibacter ruber, 687, 1051 Flexibacter rubrum, 688 Flexibacter sancti, 1051 Flexibacter species, 96.210. 643.685. 686. 739,890, 1015,1218,1803 Flexibacter tractuosus, 477, 1054 Flexibacterium Medium, 687, 688 Flexiligladius Medium, 688 Flexislipes Medium, 688 Fiexistipes sinusarahici, 688 FiexilhrLr dorotheae, All, 687, 689, 1054 Flexithrix Marine Agar, 688 Flexilhrix species. 135. 340. 349.472.610. 709. 718. 856, 1237, 1238, 1350.1539, 1574, 1594, 1597, 1626, 1629, 1640, 1667. 1669 FIGlyM Medium. 689 FLN Medium. 690 Mo Agar. 690 Fluconazole Testing Medium, 691 Flucytosine assay, 1937 Fluid Casein Digest Soya Lecithin HiVeg Medium. 691 Fluid Casein Digest Soya Lecithin Medium. 691 Fluid Lactose HiVeg Medium, 691 Fluid Lactose HiVeg Medium with Soya Lecithin and Polysorbale, 20. 691 Fluid Lactose Medium. 692 Fluid Saboraud Medium, 132 Fluid Sabouraud 1 liVcg Medium, 692. 1533 Fluid Sabouraud Medium, 692 Fluid Selenite Cystine I liVeg Medium, 692 Fluid Selenite Cystine Medium, 692 Fluid Tetralhionale HiVeg Medium without Iodine and BG, 692 Fluid ihioglycolatc Agar with Calcium Carbonate, 693 Fluid Thioglycolale Medium, 694, 1774

Fluid ITiioglycolate Medium with Beef

Extract, 694 Fluid Ihioglycolatc Medium with K. Agar. 694 Fluid Ihioglycolatc Medium with Rabbit Serum, 695 Fluid 'lluoglycolate Medium, without Glucose or FJi Indicator, 695 Fluid lliioglycollate Agar, 693 Fluid lliioglycollate HiVeg Medium, 694 Fluid 'lliioglycollate HiVeg Medium with HiVeg FIxtract. 694 Fluoranthene utilizing bacteria, 1051 Fluorescein Denitrification Agar, 696 Fluorescein production, 690, 696 Fluorescence Denitrification Medium, 696 Fluorescence I-actose Nitrate Medium, 690 Fluorescent Pectolytic Agar. 695 Fluorescin production, 1445 Fluorocult Brilliant Green. 2%-Bilc Broth. 261 Fluorocult LCD Agar. 623 Fluorocult I^auryl Sulfate Broth, 932 Fluorocult Tryplose Sulfite Cycloserine Agar, 1853 Fluorocult TSC Agar, 1853 Fluorocult* 1:. coli ()157:117 Agar, 695 Fluorogenic procedure, 623, 932,933, 1252, 1253,1254 Flurouracil Leptospira Medium, 684 FM Medium. 696 FN Medium, 696 FNA Medium, 6% Folic Acid Agar, 696 Folic Acid Assay HiVeg Medium. 696 Folic Acid Assay Medium, 696, 697 Folic Acid Casci I liVeg Medium. 698 Folic Acid Casei Medium, 698 Folic Acid Casci Medium with Chloramphenicol, 698 Folic Acid Culture Agar. 699 Folic Acid Culture HiVeg Agar, 699 Folic Acid HiVeg Medium, 699 Folic Acid Inoculum HiVeg Medium. 699 Folic AOAC Medium. 700 Fomes annosus, 700 Fomes annosus Isolation Medium No. 1, 700 Fomes annosus Isolation Medium No. 2, 700 Food ingredients, 952, 976 Food products, 1559, 1712 Food samples, 1701 Food sources, 282, 283 Food spoilage, 464, 465, 607 Food-processing equipment, 683 Foods, 11.46.47.75,76.117.151.154.162. 192.193. 194.198.212.216.220.221, 260. 261, 262, 263, 298,299, 302, 320, 336. 337. 365. 398.417.439.458.461. 464. 469. 482, 585. 588, 589. 593, 606, 607, 613, 630, 631, 657,683. 693, 705. 704. 705. 706, 731,733, 740.747. 750, 762, 775, 800, 802. 821, 831. 853, 860,

1985

877, 880, 890, 891, 905. 908.909, 916. 918, 920,922, 923,926, 932,933. 941, 952. 953.954. 955.956. 959.960. 975. 976, 978, 986, 992, 995. 1000. 1001, 1002, 1003, 1006, 1007, 1031, 1032, 1034, 1035.1037.1051,1186. 1196, 1217, 1218,1227, 1231, 1233, 1252, 1253,1254.1288.1309.1316. 1329. 1337, 1340,1345, 1346, 1349, 1350, 1361, 1370,1401, 1403, 1405, 1408, 1412.1413.1414.1415.1426. 1427. 1436, 1481, 1482, 1486, 1487, 1517, 1526, 1538.1554.1569.1570.1571. 1573, 1574. 1585, 1586. 1625. 1631. 1632, 1634,1671, 1691, 1699, 1700, 1701. 1796,1800,1804, 1819, 1821. 1822, 1824. 1826. 1829, 1830. 1831, 1832, 1833,1834, 1835, 1837, 1840, 1844. 1849.1850. 1851. 1886. 1887, 1893,1901,1902,1903,1943 Foodstuffs, 1644 Forget Fredette Agar. 700 Parnate Fumaratc Medium, 700 Formate Ricinolcate Broth, 701 Formivibrio citricus, 389, 701, 1435 Formivihrio citricus Medium, 701 Lowells Acetate Agar, 701 FPA Medium. 695,701 I RAG Agar, 702 1 ragilis Agar, 702 Francisella philomiragia, 473 Francisella tularensis, 232.473,474. 703, 731,875 Francisella tularensis Isolation Medium, 703 Frankia Agar, 703 Frankia alni. 703 Frankia Isolation Medium. 703 Frankia Medium. 703. 704 Frankia species. 607. 703, 704. 1319 Fraser Broth. 704 Fraser HiVeg Broth Base, 705 Fraser Secondary Fnrichment Bioth, 705 Fraser Secondary Enrichment HiVeg Broth Base, 705 Fraser Supplement, 6 Frateuria aurantia, 27,28, 1005, 1950 Free- living amoebae, 1301 Freezing Agar. 706 Freezing Medium, 706 Fresh water, 330. 1053 Fresh Yeast Hxtract Solution. 4 Freshwater, 213. 355.379. 578. 1186.1370. 1927, 1929 Freshwater Amoeba Medium. 706 Freshwater habitats. 213 Freshwater samples. 1927. 1929 Frcy Mycoplasma Broth Base. 707 b'riedmanniella lacustris, 635 Friis Medium,, 707 Fructose Mineral Medium, 707 KSM Selective Medium, 708

1986

Index

FIG, 1774 FIX Broth. 709 Fuchsin lactose Broth, 709 Fuels. 286 FUF Medium. 709 Fumarate Medium, 710 Fundilxtcterjadettsis Medium, 711 Fungal, 712 Fungal, 245, 246. 247, 858, 1261, 1284 Fungal Agar, 711 Fungal Agar with Low pH, 711 Fungal degradation, 155 Fungal sporulation, 1416 Fungi, 16,62, 158,236, 244,245, 246, 247, 254. 343,447, 450,451, 452,453,481, 496, 497. 586, 599. 609. 638. 701, 711, 712, 754. 761. 899. 900. 905.930,952. 959,1000,1001,1002,1025,1026,1163, 1204,1227, 12,34.1257, 1260. 1261. 1271, 1284, 1320, 1321,1322, 1337, 1338.1396.1410.1412.1413. 1416. 1417, 1527, 1528, 1529, 1530, 1531, 1532, 1533, 1648, 1683, 1707, 1710, 1802.1878. 1901.1902.1931. 1934. 1937 Fungi Kimmig Agar, 712 Fungi Kimmig Agar Base. 712 Fungi Kimmig HiVeg Agar Base, 712 Fungi Kimmig Selective Agar. 712 Fungi susceptibility test, 1937 Fungicides, 16 Fungobiotic Agar, 713 Furoale Agar, 713 Furunculosis Agar, 713 Furunculosis HiVeg Agar, 713 Fusarium acuminatum, 596 Fttsarium solani. 1286 Fusarium species, 147 Fusibacter paucivorans. 714 Fusibacter paucimrans Medium, 713 Fusobacleria, 1485 Fusobaclerium Medium, 714 Fusobacterium navi/orme, 1459 Fusobaclerium necrophorum. 332. 715.718. 1459 Fusobaclerium necrophorum Medium, 714 Fusobacterium nucleatum, 1328, 1459 Fusobaclerium prausnitzii, 367 Fusobacterium pseudonecrophontm. 631, 718 Fusobacterium simiae, 1890 Fusobacterium species, 56, 57, 369,371, 667, 696. 714, 939.963,132«, 1485. 1891 Fusobacterium varium, 431 FWM Medium, 715 FWN Medium. Modified with Fructose. 716 FWN Medium. Modified with Xylan, 717 FX A Broth. 717 FX AG Broth, 718

G Medium. 718 G25N. 755 GA Medium. 731 Galactose fermentation, 1387 (/a/hone :1a ferruginea. bib GAM Agar. 718 GAM Semisolid. 718 Ganlnerella vaginalis. 324.613, 614.719, 810, 1033,1365, 1635, 1877, 1890 Gaixlnerella vaginalis Selective Medium. 718 Ganlnerella vaginalis Selective Supplement. 6 Gas production, 181 Gassner Agar, 719 Gassner I .actose Agar, 719 Gassner Lactose HiVeg Agar. 719 Gttlze'S Medium No. 1,719 Gauze's Synthetic Medium No.l. 719 GBNA Medium. 719 GBS Agar Base, Islam, 720 GBS Medium Base, 720 GBS Medium, Rapid, 720 GC Agar, 720, 721 GC Agar Base with Blood. 723 GC Agar Base with Kellogg's Supplement, 724 GC Agar with Ampicillin, 721 GC Agar with Ampicillin and Gcnlamicin, 722 GC Agar with Ampicillin and Tetracycline, 723 GC Agar with Chloramphenicol. Tetracycline, and Ampicillin, 724 GC Agar with l>clined Supplements, 725 GC Agar with Penicillin G. 725 GC Agar with Streptomycin and Chloramphenicol, 725 GC Agar with Supplement A, 726 GC Agar with Supplement A and with VCN Inhibitor, 727 GC Agar with Supplement A and with VCTN Inhibitor, 727 GC Agar with Supplement B. 728 GC HiVeg Agar Base with Blood, 728 GC HiVeg Agar Base with Hemoglobin, 728 GC Medium. 721 GC Medium with Chloramphenicol, 730 GC Medium. New York City Formulation. 729 GC Selective Supplement. 6 GCA Agar with Thiamine. 731 GCII Agar, 729 GCII Agar with Hcmoglogin and IsoVitale X ^ l GC-Lecl™ Agar. 730 Gelatin Agar, 731 Ciclatin DI-V Agar. 732 Gelatin Infusion Broth, 732 Gelatin Iron Agar, 732

Gelatin liquefaction, 732.1315,1771. 1772 Gelatin Medium, 732 Gelatin Metronidazole Cadmium Medium. 732 Gelatin Phosphate Salt Agar. 733 Gelatin lliosphate Salt Broth, 733 Ciclatin Salt Agar. 733 Gelatin streak method, 1948 Ciclatin utilization. 87, 732 Gelatinase, 732, 733, 734 Gclatinase production. 965 Gelatinase lest Medium, 733 Gelatin-liquefying microorganisms, 732 Cielatonc. 2 Cielysale™ peptone. 2 Gemella morbillorum, 441, 1363, 1459, 1945 Gemmata ohscuriglobus, 1627, 1628 Gemmigerformicilis. 367 Gemmobacter aquatilis, 1461 General Salts Medium for listuarinc Mcthanogens, 734 Cienital. 1809 Genital treponemes, 1809 Genitourinary specimens, 1033 Genitourinary tract, 1635 Gcnlamicin Sulfate Solution, 734 Cieo Medium. 734 Geoalkalibacter Medium,, 735 Geoalkalibatter species. 735 Geohacillus toehii, 1469 Geobacter bremensis. 736 Geobacter chapellei, 736 Geobacter grbiciae. 736 Geobacter hydrogenophilus, 736 Geolkicter Medium. 735. 736, 737 Geobacter metallireducens, 103, 736, 737 Geobacter spccics736 Geobacter sulfurreducens. 736, 737 Geococcus nitratireducens and Petrobacter succinimandens Medium., 737, 738 Geococcus nitratireducens, 738 Geodennatophilus obscurus. 210. 682. 735. 738,1318,1321, 1574 Geodennatophilus obscurus Medium. 738 Geodennatophilus obscurus subspecies ufahensis, 1929 Geomyces fxmnonis, 706 Geomyces pulvereus, 988 George's Medium, Modified, 738 Geospirillum species. 1665 Geothermobacter Medium. 739 Geothermohacler species, 739 Geothrixfermentans. 736 Geovibrio thiophilus DSM 11263.1211 Germ tubes, 1799 Germination of spores, 439 CiFY Agar. 739 Giardia cati, 893 Giardia intestinalis, 893 Gillies Agar No. 2, 740 Ciingcr Beer Plant Medium, 740 Giolitti-Cantoni Bioth, 740

Index

Giolitti-Cantoni Broth Base with Tellurite, 740 Ciisa Agar, 740 Gisa Broth, 740 Glaucoma chattoni. 1589 Glauconema bermudense, 1556 Gliding bacteria, 741 Gliding Medium, 741 Globicatella sanguinis. 441, 1800 (ilobicatella sulfulifaciens, 251 Gloebacier species. 213 Gloelxicler violaceus, 214 Gloeocapsa species, 213, 214 Glomerella cingulata. 431. 952, 1410 Glucan production. 1652. 1653 Gluconacelobacier azotocaplans, 741 Glucanacelohacter enianii, 59 Gluconacetofyacier johannae, 741 Gluconacelobacier johannae and Giuconacetobader azotocaptans Medium. 741 Gluconacelobacier rhaeticus, 741 Gluconacelobacier rhaeticus Medium,. 741 Gluconacelobacier species, 59, 1486 Gluconaceiof>acier xylinus Medium, 741 Gluconacelobacier xylinus .vuhsp. xylinus, 741 Gluconate Peptone Broth, 741 Gluconate Test IliVeg Medium, 741 Gluconobacter Agar, 741 Gluconohacter asaii, 1005, 1591, 1932 Gluconobacter Broth, 742 Gluconobacter cerinus. 27, 28, 1005, 1932 Gluconobacter frateurii. 1591, 1932 Gluconobacter Medium, 59 Gluconobacter oxydans. 27.28, 586, 742. 1005,1342,1429, 1932,1933 Gluconobacter oxydans Agar. 742 Gluconobacter Apecies. 28, 1932 Glucose Agar, 742 Glucose Agar with 25%Glucose, 742 Glucose Agar. 9K. 742 Glucose Asparagine Agar, 743, 746 Glucose Asparagine Agar, 2,743 Glucose A/ide Broth, 743 Glucose A/ide IliVeg Broth, 743 Glucose Blood Liver Agar. 225 Glucose Broth, 743, 744 Glucose Broth, 9K. 744 Glucose Broth, Buffered, 744 Glucose Cysteine Agar Base with Thiamine and I lemoglobin, 744 Glucose Cysteine I HVeg Agar Base with Iltiamine and Blood, 745 Glucose Cysteine I liVeg Agar Base with 'Iliiamine and I lemoglobin. 744 Glucose fermentation, 606,673, 1386, 1387, 1524. 1819.1820. 1845.1846 Glucose IliVeg Agar Base, Fmmons, 1532 Glucose IliVeg Broth. 745 Glucose IliVeg Peptone Agar, 745 Glucose Medium Nakayama, 745 Glucose Nitrogen-Free Salt Agar, 745

Glucose Nitrogen-Free Salt Solution, 746 Glucose Nutrient Agar, 746 Glucose Peptone Agar, 746 Glucose Peptone Medium, 746 Glucose Peptone Yeast Fxtract Salts Medium, 759 Glucose Phospliale Broth. 746 Glucose Salt Teepol Broth, 747 Glucose Salt Tccpol IliVeg Broth, 747 (ilucose Salts Medium, 747 Glucose Starch Agar. 747 Glucose Tetrazolium Medium, 747 Glucose Tryptone Yeast Fxlract Medium, 762 Glucose Yeast Broth with Sodium Chloride, 747 (ilucose Yeast Chalk Agar, 748 (ilucose Yeast Fxlract Agar. 748, 751 Glucose Yeast Fxtract Iron Agar, 748 Glucose Yeast Extract Medium. 748. 749 Glucose Yeast Extract Peptone Agar, 749 (ilucose Yeast Fxlract Peplone Agar wilh 2% Glucose, 749 Glucose Yeast Fxlract Peptone Medium, 749 (ilucose Yeast Fxtract Peptone Ibioglycolate Medium, 769 Glucose Yeast Medium with Calcium Carbonate, 749 (ilucose Yeast Peptone Medium. 749. 750 (ilucose Yeast Plant Peptone Agar, 749 (ilutamale fermentation, 410 (ilutamate Medium, 750 (ilutamale Starch Phenol Red Agar Base, 750 Glutamine Medium, 751 Glutarate Medium, 751 Glutaratc utilization, 751 Glycerol, 25% Nitrate Agar, 755 Glycerol Agar. 751. 752 Glycerol Arginine Agar, 752 Glycerol Asparagine Agar, 886 Glycerol AsjNiragine Meat Agar, 752 Glycerol Asparagine Medium. 752 Glycerol Beef Extract Medium, 752 Glycerol Calcium Malate Agar. 752 Glycerol Chalk Agar, 753 Glycerol Com Steep Agar. 753 Glycerol Glycine Agar, 753 Glycerol Nutrient Agar. 753 Glycerol Peptone Agar, 754 Glycerol sensitive, 968 Glycerol Soil Agar, 754 Glyccrol-Fnrichcd Medium. 753 Glycerol-Iuuiched Medium with 2% Ftha' nol, 753 (Jlycerol-Free Medium. 753 Glycine Cycloheximide Phenol Red Agar, 754 Glycine utilization, 274 (ilycocholale Mineral Medium, 754 Glycomyces harbinensis, 1321. 1323 Glyvomyces species, 1318 Glycomyces tenuis. 1368

1987

GMC Medium. 732 GN Broth. Hajna. 755 GN HiVeg Broth, 755 GNYS Agar, 755 GNYS Medium. 755 GOT Medium, 756 GOLCHI1 Medium. 756 Goniomonas species, 1015 Gonocoecus Medium, 756, 757 Gorbenko Medium. 758 Gordona bronchialis. 743. 754. 1330. 1368 Gordona rubropertinctus, 743, 751, 758, 767, 1330 Gordona species. 967.968, 1404 Gordona sputi, 1330 Gordona lerrae, 743, 754, 758, 899, 1330, 1368 Gordonia amicalis, 215 Gordonia ruhripertincla, 1175 Gotham's Medium for Algae, 758 GP Agar, 758 GPHF Agar. 758 (iPS Agar, 733 (iPS Broth, 733 GPVA Medium, 758 GPY Salts Medium, 759 GPY/10 Medium. 759 Grace's Insect Medium, 759 Gracilibacillus dipsosauri. 1822. 1833 Gracilibacillus Medium. 759 Gracilibacillus species. 760 Grahametta Medium. 760 Grahamella species, 760 Gram-negative anaerobes. 1906 Gram-negative anaerobic, 1550 Gram-negative bacilli, 1350. 1393 Gram-negative bacteria 153, 201,333, 343, 377. 446. 598,636.637,817.818, 821. 950, 991,1220,1221, 1224, 1225, 1289, 1290. 1291,1292.1392.1514,1520, 1539,1550,1572 Gram-negative cocci, 473 Gram-negative enteric bacilli, 201,446,598, 606,636,637.817,818.950,1191,1220. 1221,1223,1226,1393, 1409, 1410, 1524 Gram-negalivc nonfermentalive bacteria, 1225,1316 Gram-positive bacteria. 442.443.444.1384. 1485 Gram-positive cocci. 442,443.444,1538, 1641 Granada Medium, 760 Granulibacter Medium, 760 Granulihacier species, 760 Graphis tenetla, 951 Graphium fmgrans. 952 Gray's Agar. 760 Green sulfur bacteria, 1380 Green Top Agar, 761 Green Yeast and Mold Broth, 761

I9sx

Index

Ciroup A Selective Strep Agar with Sheep BlOOd, 761 Group A streptococci, 1799, 18()0 Group A streptococcal. 1669 Group B streptococci, 720, 881, 1669 Ciroup B streptococci, 1826, 1827,1835 Group B Streptococci Agar. 720 Group B Streptococci Medium. 720 Group B Streptococcus, 1641 Group D streptococci, 597 Group D streptococci. 1201 Group D-slreptococci, 648 GS Agar, 733 GS Medium. 761 GSP Agar. 762 GS IB, 747

H 2 S production. 273, 817. 818, 903. 904, " 939, 964, 975, 976,977,1042, 1043, 1055.1225,1364, 1514. 1520,1537. 1572, 1625,1658, 1819, 1820 HA, 800 HAEB.853 Haematococcus lacustris, 70 Haemophilus actinomycelemcomilans. 215. 774 Haemophilus Agar, 772 Haemophilus ducreyi, 772, 817, 1476 Haemophilus ducreyi Medium. 772 Haemophilus ducreyi Medium, Revised, 772 Haemophilus haemoglobinophilus, 774 Haemophilus Identification Quadrant Plate

GTC Agar Base with Bicarbonate, 762 GTYE Medium. 762 (iuanosiuc Medium 762 Guaymas basin, 150 Guizotia abyssinica Creatinine Agar, 223 Gum Base Nalidixic Acid Medium, 719. 762 Gum Listeria Medium, 762 Gum 1 raeacanth Gum Arabic Medium, 762 Guttman's 11B Medium. 763 GV Medium. 763. 764. 765. 766 GY Agar, 767 GY Double Strength Agar with Uracil and Uridine, 767 GYE Medium. 767 GYEP Medium, 767 GYM + Seawater, 767 GYM Starch Agar, 767 GYM Starch Medium, 768 GYM Streptomyces Agar. 768 GYM Streptomyces Medium, 768 GYM+S Agar. 767 Gymnascella citrino. 706 Gymnoascoideus pelalosporus, 706 Gyinnoascus intermedins, 706 Gymnophrydium marinum. 644 GYP Agar. 768 GYP Medium, 749 GYP Sodium Acetate Mineral Salts Broth,

Haemophilus influenzae. 324,325,346, 347, 681.722.773.774.1250 Haemophilus influenzae Defined Medium MI, 773 Haemophilus influenzae Defined Medium Ml-Cit, 773 Haemophilus Medium, 773 Haemophilus paragallinarwn, 251, 772, 774 Haemophilus parainfluenzas 721. 722. 723, 725 Haemophilus paraphrophilus, 17 A Haemophilus- parasuis, 774 Haemophilus pisciuin, 601. 1842, 1843 Haemophilus segnis. 774 Haemophilus somnusy 774 Haemophilus somnus Agar, 774 Haemophilus species. 107. 360, 361. 721, 723. 727. 728. 729. 730. 820.929.950. 951 Haemophilus Test Medium. 774 Haemophilus vaginalis, 1033. 1635 llafniaalwi. 1309,1928 Hafnia species, 496 Hagedom and Holt Selective Medium. 774 Hagcm's Modess Medium, 775 Haladaptatus paucihalophilus, 802 Halanaerobium alcaliphilum, 779 Halanaerobium lacusrosei. 780 Hall'Fraser Broth without Pontic Ammonium Citrate, 775 Hall" Praser Broth, 775 Haiiangium ochraceum, 1899 Haiiangium tepidum. 1899 Haliphthoros milfordensis, 287, 1825 Haliphthoros philippinensis. 287 Haliscomenohacler hydrossis, 776, 1553 Haliscomenobacter hydrossis Medium, 775 Haliscomenohaeter Medium, 775, 776 Haliscomenolxicter species, 776 Haloahacteroides acunaris, 790 Haloalkaliphilic Agar. 777 I Ialoalkaliphilic Growth Medium,, 777 Haloanaembacter chilinovorans. 778 Haloanaerohaeter chilinovorans Medium,

768 GYP Sodium Acetate Mineral Salts Broth with 5% Sodium Chloride. 769 GYP Sodium Acetate Mineral Salts Broth with Sodium Chloride, 768 GYPT Medium, 769 Gyrodon lividus, 1199 Gyromitra species, 1002

B H Agar. 769 11 agglutination antigen. 770 II Broth, 770 II Diphasic Medium. 771 II Medium. 771 H Top Agar. 771 U.S. Vaccine HiVeg Medium, 1632

with Growth Factors, 820

777, 778 Haloanaembacter lacunaris, 791

Haloanaerobium alcaliphilum Medium, 778 Haloanaerobium congoiense Medium, 779 Haloanaerobium lacusroseus, 780 Haloanaerobium lacusroseus Medium, 780 Haloanaerobium Medium. 780 Haloanaerobium praemlens, 781, 783 Haloanaerobium praevalens Medium, 781 Haloanaerobium saccharolyticuni, 789 Haloanaerobium salsugo Medium, 781 Haloanaerobium salsulgo, 782 Haloarcola Medium. 782 Haloarcola calijforniae, 1346 Haloarcola hispanica, 787.798. 1346 Haloarcola japontca, 782, 1346 Haloarcola japonica Medium. 782 Haloarcola marismortui, 782, 788. 1346 Haloarcola marismortui Medium, 782 Haloarcola sinuiiensis, 1346 Haloarcola species, 783, 784, 785 Haloarcola vuUismortis, 345,783.786,1346, 1926 Haloarcola vallismortis Synthetic Medium, 783 Halobacillus halophilus. 1009 Halobacillus litoralis, 783 Halobacillus Medium. 783 Halobacillus trueperi. 783 Halobactcria, 784 1 lalobactcria Agar, 783 I lalobacteria Medium, 783, 784 I lalobacteriaceae, 784, 785 I lalobacteriaceae Medium, 1,784 I lalobacteriaceae Medium, 2, 784 I lalobacteriaceae Medium, 3, 784 I lalobacteriaceae Medium. 4. 784 Halohaclerium Agar, 785 Halobacterium culirubrum. 786. 1346 Halohaclerium denitrificans, 785 Halobacterium denitrificans Medium. 785 Halobacterium distribution, 1346 Halobacterium halobium. 785. 786. 799 Halobacterium halobium Defined Medium, 785 Halobacterium halobium/ Halobacterium salinarium Medium, 786 Halobacterium lacusprofttndii. 1346 Halobacterium lacusprofundii Medium, 786 Halobacterium Medium, 786, 787 Halobacterium pharaonis, 788 Halobacterium pharaonis Medium, 787 Halobacterium saccharovorum, 786, 788, 1346 Halobacterium saccharowrum Medium, 788 Halobacterium salinarium, 786, 787, 799, 1346 Halobacterium simoncinii, 1346 Halobacterium soldomese. 788 Halobacterium soldomese Medium, 788 Halobacterium species. 322. 783. 785. 802. 1346. 1926 Halobacterium Starch Medium, 788 Halobacterium trapanicum. 799, 1346

Index

llalobacterium volcanii. 788, 1346 llalobacterium volcanii Medium, 788 Halobacterium-Halococcus Medium, 786 I'lalobacteroides acetoethylicus, 789, 1858 I lalobacteroides acetoethylicvs Medium, 788 I lalobacteroides halohius, 790 I lalobacteroides Medium, 790 Halobacteroides/llaloincola Medium, 789 Halobaculum gomoirense. 791 Ilalobaculum gomorren.se Medium, 791 Halobius Medium, 791 /falocella cellulolytica, 792 Haloed la cellulolytica Medium, 791 I!aloeocci,784 Halococcus Agar, 792 Halococcus dombrowskii, 792 Halococcns dombrowskii Medium, 792 Halococcus moirhuae, 783, 785, 786, 792, 1346. 1926 Halococcus nondemtrificans, 1555, 1556 Halococcus saccharolyticus, 1200. 1346 Halococcus species, 784, 1311. 1346 Halococcus turkmenicus, 1346 Halodurans Medium, 792 llaloferax denitri/icans, 785.1346 llaloferax gibbonsii, 787, 798. 1346 llaloferax gomorrae, 791 llaloferax mediterranei. 785,787,793,1346, 1420 llaloferax mediterranei Medium, 792 llaloferax mediterranei Minimal Medium I, 793 llaloferax mediterranei Minimal Medium 11. 793 llaloferax species. 783, 784 llaloferax sulfurifontis, 793 llaloferax suifurifontis Medium.. 793 llaloferax volcanii, 787, 793, 794, 1846 llaloferax volcanii Low-Salt Medium, 793 llaloferax volcanii Medium, 793 llaloferax volcanii Minimal Medium. 794 llaloferex volcanii, 785 Halogeometricum borinquen.se. 784 Haloincola saccharolyticum. 789 llalomethanococcus Medium, 794 llalomicrobium katesii. 795 Halomicrobium katesii Medium,, 795 llalomicrobium mukoltalaei, 784 Haiomonas campisalis, 111 Halomonas desiderata. 795. 796 Haiomonas desiderata Medium, 795 Halomonas elongata. 796, 1346, 1617 Haiomonas eurihalina, 1154 Halomonas halmophila, 799, 1343. 1617 Halomonas magadiensis, 796 Halomonas magadiensis Medium. 796 Halomonas Medium, 796 Halomonas meridiana, 136, 148 Halomonas panlelleriense, 7%, 797 Halomonas panlelleriense Agar. 796 Halomonas panlelleriense Medium, 796

Halomonas pantellertensis, 796, 797 Halomonaspantelleriensis Medium, 797 Halomonas species. 77,136. 1009, 1558 Halomonas subglaciescola, 797, 798 Halomonas subglaciescola Medium, 797 Halonatronum saccharophilum, 798 llalonatronum saccharophilum Medium, 798 Halophilc Agar, 798 HalophileAgarl, 799 Halophile Medium. 799 Halophilc Medium III, 799 Halophiles, 960 llalophihc Agar. 800 llalophihc archaea, 1053 llalophihc Bacillus species. 1053 llalophihc bacteria, 322, 803,960, 1556. 1926 llalophihc Broth, 800 llalophihc Chromatium Medium. 800 llalophihc Clostridium Agar, 800 llalophihc Clostridium Broth. 801 llalophihc cyanobacteria. 326, 1948 llalophihc Desulfovibrio species. 1638 llalophihc fungi, 1415 llalophihc 1 Ialobactcrium Medium. 802 llalophihc HiVeg Agar, 802 llalophihc HiVeg Broth, 802 llalophihc Medium, 802 llalophihc Methanotrophic Bacterium Medium. 802 llalophihc microorganisms, 800, 802, 1168, 1924 llalophihc Nutrient Agar, 803 llalophihc Rhodomicrobium species. 1879 llalophihc Synthetic Medium, 803 llalophihc Vibrio species. 264. 375. 813. 817, 1237, 1249, 1386, 1387 llalophihc vibrios, 1336 llalophihc yeasts. 1415 llalophylophlhora masleri, 1878 Halopiger Medium. 803 Halopiger species, 803 Haloplanus natans. 1484 Haloquadratum walshyi, 803 Haloquadratum walsbyi Medium. 803 Halorhabdus utahensis, 804 llalorhabdus utahensis Medium. 803 Halorhodospira Medium, 804 Halorhodaspira Medium with Succinate. Acetate, and Yeast Hxtract. 804 Halorhodaspira sj>ecies, 804, 805 Halorubnim californiense. 805 Halorubnnn californiense Medium, 805 llalorubrum lacusprofundii. 786 flalomhrum sjxxries, 784 Halosarpheia retorquens, 1555, 1557 Ilalosphaeria quadricornuta, 638 Halosphaeria salina. 287 Ilaloterrigena species. 784 Halolhennothrix orenii. 806 Halolhermothrix orenii Medium, 805

1989

Halothiobacillus halophilus DSM 6132. 1768 Halothiobacillus kellyi, 806 Halothiobacillus Medium. 806 Ilalovibrio Medium. 806 llalovihrio species, 806 Ilalovibrio variabilis, 800, 807 Ilalovibrio variabilis Medium. 807 Ham's F-10 Medium. 807 Hanahan's Broth, 807 Hanahan's HiVeg Broth, 808 IIanseniaspora uvarum. 1942 I lapalosiphonfontinalis. 214 HarlcquinTBGA. 1837 Harpo's HTYF Tryptieaseâ&#x201E;˘ Peptone Medium. 808 Harpo's IITYKM Marine Medium, 808 Harrold's Agar, 988 Hartley's Digest HiVeg Broth. 808 Hartley's Digest Broth. 808 Hartmannella Umax, 706

llartmannella venniformis. 706, 1217 Haskins Agar for Tetrahymena. 808 HAY. 809 Hay Extract Agar, 809 Hay Hxtract Medium, 811 Hay Infusion Agar, 809 Hayflick Medium, 809 Hayflick Medium. Modified. 809 I la/ardous Components, 9 I Hi, 800 HBTBilayer Medium. 810 HC Agar. 820 HC Agar Base, 810 HC Agar with MUG, 821 HD Agar. 1:10 Diluted, Modified. 810 HD-Mcdium. 1:10 Diluted. Modified. 811 HE Medium, 811 Heart Infusion Agar, 811 Heart Infusion Agar (pll 7.6) with Inactivated Horse Serum, 812 I leart Infusion Agar with 0.1 % Glucose, 812 Heait Infusion Agar with Glucose, 812 Heart Infusion Agar with Horse Serum and Fresh Yeast Fxtract, 812 1 leart Infusion Agar with Inactivated I lorse Serum, 813 Heart Infusion Agar with Inactivated I lorse Serum, NaCl and Penicillin, 813 Heait Infusion Agar witli Rabbit Mood, 813 Heart Infusion Agar witli Sodium Chloride, 813 Heart Infusion Agar with Yeast Fxtract, 813 Heart Infusion Agar. HiVeg. 812 Heart Infusion Agar. HiVeg with Blood. 812 Heart Infusion Broth, 813. 814 Heart Infusion Broth (pll 7.5) with Inactivated 11 -.1111.111 Scrum and Yeast Hxtract. 814, 816 I leart Infusion Broth with Additives for Staphylococcus, 814

1990

Index

Heart Infusion Broth with Additives lor Streptobacillus, 814 Heart Infusion Broth with Glucose. 814 Heart Infusion Broth with Glucose and Antibiotics. 814 Heart Infusion Broth with I lorsc Serum and Fresh Yeast Fxtracl. 815 Heart Infusion Broth with Inactivated Horse Serum, 816 Heart Infusion Broth with Inactivated Horse Serum and Fresh Yeast Extract, 816 Heart Infusion Broth with Inactivated Horse Serum, Fresh Yeast Extract, and Sucrose, 816 I leart Infusion Broth with Porcine Serum and Fresh Yeast Fxtracl, 816 I Icart Infusion Broth with Sodium Chloride, 817 Heart Infusion Broth. HiVcg. 815 Heart Infusion Medium with Fetal Bovine Serum. 817 Heart Infusion Tyrosine Agar, 817 Heat-resistant fungi, 469, 482 Hcbcloma crustuliniforme. 1199 Hebcloma pusillum, 1199 Hcktoen Enteric Agar, 817 Hektoen Fnteric Agar. HiVcg, 818 I lei a cell lines, 439 HeLa cell test, 439, 875, 878 Hcta tissue culture. 336 Helcococcus hmzii, 215, 251 Helicascus kanaloamts, 638 Helicobacterj'elis, 818 Helicobacter Medium. 818 Helicobacter mundarum, 818 Helicobacter nemestrinae, 269 Helicobacter pylori, 269, 306, 721, 818, 819 Helicobacter pylori Isolation Agar. 818 Helicobacter pylori Selective Medium, 819 Helicobacter pylori Selective Supplement. 6 Helicococctis kunzii. 247 Helicodendron lubulosum, 452 Helicoma morganii, 348 Helicoon richonis, 348 Helicusporium pallidum, 1877 Heliobacillus mobilis, 819, 1880 Heliobacillus mobilis Medium. 819 Hetiobacterium chlorum, 819, 1926 Heiiubactertum chlorum Medium, 819 Heliobacterium modestocaldum, 1455 Heiioresfis baculata. 820 Hetiorestis Medium, 819 Helkesimastixfaecicola. 1556 Helminthosporium carbonum, 1410 Helminthosporium papillosum. 1877 Helminthosporium solini, 1410 Hemin Medium for Mycobacterium, 1162 I lemin Solution, 4 I Icmmcs Medium Base. 820 Hemo II) Quad Plate, 820 Hemo ID Quad Plate with Growth Factors. 820

1 lemoglobin. 4 Hemoglobin Solution, 2%, 4 Hemolysis, 719 Hemolytic, 227, 228, 229, 230, 811, 812, 814.1307.1384.1397.1570,1826,1827, 1835

Hemolytic Streptococcus, 1642 Hemorrhagic coli Agar, 820 I lemorrhagic Coli Agar with MUG. 821

Heparin Medium, 821 Herbaspirilluni Agar. 821 Herbaspirillum rubrisulxtlbicans. 1306 Herbaspirillum seropedicae, 821, 826, 827, 828,829. 1602 Herellea Agar, 821 Herellea species. 821 Herellea vaginicola, 1561 Hermoanaerobacter sulfurophilus. 1715 Hermococcus peptonopbilus, 1022 Hermoerinis ruber. 1331 HerpeUnnonas anglusteri. 600 Herpetomonas mariiuleanei. 600, 958 Herpetomonas megaseliae, 600, 979 Herpetomonas muscarwn. 600 Heqyetomonas roitmani, 600 Herpetomonas samuelpessoai, 461 Herpetomonas species, 912 Herpctosiphon aurantiacus, 1575 Herpetosiphon eohaerens, 1054 Herpctosiphon geysericola, 890, 1051 Herpetosiphon glganteus, 822 Herpetosiphon giganleus Medium, 821 Herpetosiphon nigricans, 1054 Herpetosiphon persicus, 1054 Herpetosiphon species, 135, 340,349,472, 475, 610, 709, 718, 856, 1237, 1238,

1350, 1539.1574. 1594.1597.1626, 1629, 1640, 1667, 1669 Hershey's Tris-BulTered Salts Medium, 822 HESNW Medium, 822 HESP1/SRI/TMC4/1.UP Medium. 822.823. 824.825 Heteramoeba clara, 1011 Heterotrophic Agar 113 P. 826 Heterotrophic Broth IBP. 827 I leterotrophic I lypcrthcrmophilic Archaea Medium, 828 I leterotrophic Medium for Hydrogenomonas, 829 I leterotrophic Medium for I lydrogert-Oxidi/ing Bacteria, 829

Heterotrophic Medium EBP, 829 I leterotrophic Nitrolxicter Medium. 830 1 leterotrophic Plate Count Agar, 857 I leterotrophic plate count technique, 857, 1318 Hexamita inflata, 831 Hexamita Medium, 831 Hexamita pusilla, 831 Hexamita species. 893 HUD Medium. 831 111,814

HIwithNaCl,817 HIA.8II HIA with NaCl. 813 I lickey Trcsner Agar, 831 1 liCrome MacConkey-Sorbitol Agar. 839 IliCrome MacConkey-Sorbitol Agar with Tellurite-Ccfixime Supplement. 840 I liCrome Nickels and I .eesment Medium, 842 IliCrome™ Aureus Agar Base with Fgg Tellurite. 832 1 liCrome IM BaaHits Agarwtih Polymyxin B, 832 IliCrome1 M Candida Agar. 832 IliCrome™ Candida Agar. HiVcg, 832 HiCrome IM Candida Differential Agar Base with Candida Selective Supplement, 833 I IiCrome ,M Candida Differential Agar Base. Modified with Candida Selective Supplement. 833 I liCrome™ Candida Differential Agar Base, Modified wtih Candida Selective Supplement. 843 IliCrome™ Colifonn Agar with SLS, 834 1 liCrome1 M Colifonn Agar with SI.S and

Novobiocin, 834 I liCrome™ Coliform I IiVcg Agar wtih SLS. 834 IliCrome™ E coli Agar. 834.835 IliCrome™ E. coli Agar, HiVeg, 835 HiCromc™ EC 0157:H7 Agar. 835 HiCrome™ F.CC HiVeg Agar, 836 I liCrome™ FCC Selective Agar Base. 837 I liCrome™ BCD Agar with MUG, 837 I liCrome1 M FCD HiVcg Agar with MUG. 837 I liCrome™ Fjirichmcnt Broth Base for EC 0157:117.838 HiCrome™ Enterohacter sakazakii Agar, Modified. 838 IliCrome™ Fnterococci Broth, 838 HiCromc™ Fnterococci HiVcg Broth. 838 I liCrome™ Enterococcus faecium Agar Base. 838 HiCrome™ Improved Salmonella Agar, 839 I liCrome™ Klebsiella Selective Agar Base with Carbenicillin. 839 HiCromc™ Listeria Agar Base, Modified. with Moxalactam. 839 HiCrome™ M-CP Agar Base. 840 HiCrome™ MeReSa Agar with Methicillin. 840 I liCrome™ McRcSa Agar with Oxacillin. 841 HiCromc™ Miller and Mallinson Agar. 841 HiCrome™ M-Fauryl Sulfate Agar, 841 HiCrome IM MMAgar.841 HiCrome™ MM HiVeg Agar, 841 HiCrome™ MS.0157 Agar, 841 1 liCrome™ MS.Ol 57 Agar with Tellurite. 842 HiCrome™ M-TFC Agar. 842

Index

HiCrome™ OGYF Agar Base, 842 HiCrome™ RajHans Medium, 843 1 liCromc1 M Rajl laus Medium. Modified, 843

HiCrome™ Rapid CoUfonn Broch, 843 HiCrome1 M Rapid Fnterococci Agar. 843 HiCrome™ Salmonella Agar, 843, 844 HiCrome™ Salmonella Chromogen Agar, 844 HiCrome1 M Salmonella Medium. Modified. 1478 HiCrome™ UTI Agar. HiVeg. 844 HiCrome™ LI I Agar. Modified, 844. 845 HiHuoro™ Pseudomonas Agar Base, 845 High level aminoglycoside resistant entcrococci, 1895 High Plate Count Agar, 845 High Salt Nutrient Agar, 845 Higli Salt Peptone Yeast Extract Agar, 845 HiobaciUus ihiopams. 1766 tftppea maritima. 846 Hippea Medium, 845 Hippurale Broth. 1585 Hippurate hydrolysis, 1585 I lippurale 1 lydrolysis Bn>th, 846 Hippurate hydroly/ation. 1646 Ilippurate hydroly/ing lwctcria, 846 Ilirschia hallica. 846 Ilirschia Medium, 846 1 li-Scnsitivity Test Agar. 846 I li-Sensitivity Test Broth, 847 Hi-Sensitivity Test HiVeg Agar. 847 Hi-Sensitivity Test HiVeg Broth, 847 Hisitcsl Agar. 848 Histidans Agar, 848 1 listolytic Clostridia, 940 Histoplasma capsulalum, 244,245,251,254, 749, 849, 850, 1399, 1528, 1586,1928 Histoplasma capsulalum Agar, 848, 849 Histoplasma capsulalum Broth, 850 Histoplasma duboisii, 849. 850 Ilistoplasmin, 153 Histotoxic Clostridia. 930 I IiVeg Acid I lydrolysate No.l, 3 1 IiVeg Extract, 3

HiVeg Extract No. 1,3 HiVeg Ex tract No. 2.3 I IiVeg I lydrolysate, 3 I li Veg I lydrolysate Agar with 2.5% Agar. 850 HiVeg Hydrolysate C, 3 HiVeg Hydrolysate No. 1, 3 I IiVeg I lydrolysate No. 2,3 HiVeg Hydrolysate No. 3,3 HiVeg Hydrolysate No. 4 , 3 HiVeg Hydrolysate No. 6. 3 I IiVeg Infusion, 3 I IiVeg Infusion No. 1.3 I IiVeg Infusion Powder, 3 HiVeg Magnesium Broth. 851 I IiVeg Peptone, 2 HiVeg Peptone B. 2 HiVeg Peptone C, 2

Hi Veg Peptone No. 1,2 HiVeg Peptone No. 2, 2 HiVeg Peptone No. 3,2 HiVeg Peptone No. 4 , 2 I IiVeg Peptone No. 5.2 HiVeg Special Infusion, 3,4 HiVeg Special Peptone, 2 I IiVeg Yeast Hydrolysate, 4 HI. Agar. 851 HI.ARK, 1895 HM Medium. 851 HNS Agar, 851 HNVV Medium, 852 I loagland Trace B lement Solution, Modified, 4 Holer's Alkaline Medium. 852 Holm's Medium. Modified, 1638 Holding Medium, 297. 309, 319, 1626 1IO-I.F. Trace I-lement Solution, 853 Holophaga foetida. 1797 I lominis Agar, 769 Hominis Broth. 770 Horie Arabinose P.thyl Violet Broth, 853 Horikoshi-1 Medium with 10% Sodium Chloride, 853 Horikoshi Alkaline Medium. 853 Horikoshi-1 Medium,, 853 Hormoconis resinae, 585, 586, 587, 742 Horse Blood Agar, 854 Horse Blood, Citrated, 4 Horse Blood, Dclibrinatcd. 4 1 lorse Blood, 1 lemolysed, 4 I lorse Blood, Oxalated. 4 Hone Serum, 4 Horse Scrum Agar. 854 I lorse Serum Broth, 854 Horses. 1274 Host cells, 1345 I lollingcr Broth, 854 Howardella Medium, 854 Howardella species. 854 Hoyer's Medium, 854 Hoyle HiVeg Medium Base, 855 Hoyle Medium, 855 Hoyle Medium Base, 855 HP. 6 Agar. 856 HP, 6 Agar Base, 856 HP. 74 Broth. 856 HP Medium, 856 HP101 Halophile Medium. 856 I IPC Agar, 857, 1318 HQG6T Medium. 857 IIR Antifungal Assay Medium Buffered wim MOPS. 858 HS Hi Veg Medium, 858 IIS Medium, 858 HTM. 774 Hugh Leifsoo Deoxycholate HiVeg Agar, Modified. 943 Hugh Leifson Glucose Broth. 858 Hugh Ijeifson Glucose HiVeg Medium, 859 Hugh Ijcifson HiVeg Medium, 859

1991

Hugh I.eifson's Oxidation Fermentation Medium, 1335 Human, 304. 1270 Human blood, 202,252 Human Blood Twccn Bilaycr Medium, 810 I luman fecal specimens, 298 Human immunodeficiency viruses. 1520 Human mycoplasmas, 1270 Humieola fuscoatra, 1348 Humicola grisea, 1348 Hungalc's Habitat-Simulating Medium. 859 1 turner's Medium For Euglena, 860 I turner's Medium for Euglena, 860 I turner's Mineral Base. 4 1IY Agar for I-'lawbacierium, 860 IIY Medium for I'lavobacterium, 860 HYA Agar, 860 Hydrocarbon utilization. 286, 1048 HydrocarlM>n-utili/ing bacteria, 156, 1053, 1327 Hydrogen sulfide production, 903,904 Hydrogenivirga caldiiitoris. 852 Hydrogenivirga okinawensis. 861 Hydrogcnivirga okinawensis Medium,, 861 Hydrogenohacler acidophilus, 862 Hydrogenobacter acidophilus Medium, 861 Hydrogenohacler halophilus Medium, 862 Hydrogenohacler thermophiius, 862, 1730 Hydrogenobacter thermophiius Medium. 862 Hydrogenobaculum acidophilum. 862 Hydrogenoflava palleromi. 1304 Hydrogenomonas species. 829 Hydrogenophaga Jlava, 1177, 1304 Hydrogenophaga pseudojlava. 1177 Hydrogenophilus hirschii, 863 Hydrogenothermophilus hirschii, 863 Hydrogenothermus marinus, 1891. 1892 Hydrogeno\ihrio marinus, 862 Hydrogen-oxidizing bacteria, 861,1701 Hydrogen-oxidizing Medium, 861 Hydrophobic grid membrane filter method. 975 I lydroxybcnzoalc Agar. 863 Hydroxyben/oale Broth, 863 I tydroxybenzoate Medium, 864

Hydroxybenzoic Acid Medium, 865 Hydroxybiphenyl utilizing bacteria, 1177 1 tydroxybutyrate Medium, 865 Hygrophorous purpurascens, 1199 Hygrophoms russula, 1199 Hymenohacter aerophilus, 1456 Hymenobacler species, 1456 Hyperthennophilic archaea, 829 Hyperthermus butylicus. 866 Hyperthermus butylicus Medium, 865 Hyphomicrobium aestuarii. 435. 444. 866 Hyphomicrohium Fnrichment Medium, 866 Hyphomicrobium factlis, 435,444.866 Hyphomicrobium hollandicum. 866 Hyphomicrobium indicum, 1009 Hyphomicrobium Medium, 866

1992

Index

Hypfiumicrohium Medium 337a. 866 Hypfwmicrobium methylovonnn, 867,868, 869 Hypfwmicrobium methylovorum Medium, 866 Hypfwmicrobium species, 435, 444, 594, 866.867, 1112.1173, 1197 Hypfwmicrobium Strain X Agar. 867 Hypfwmicrobium Strain X Bnilh, 867 Hypfwmicrobium stdfoniwrans. 1148, 1149 Hypfwmicrobium variahile, 435,444 Hypfwmicrobium vidgare. 866. 1112 Hypfwmicrobium zavarzinii, 435,444, 866 Hypfwmonas Hnrichmenl Medium. 867 Hypiwmonas Medium. 867 Hypfwmonas polymorpha, 867 Hypfwmonas species. 867, 943 Hypolricfwmonas acosta, 1861 Hypoirichomonas species. 1861 I lypoxanlhine Agar, 867 1 lypoxanlhine hydrolysis, 868 I IBB Agar, 877 Idiomarina Medium., 868 Idiomarina speeies, 868 IE Medium. 868 IFO Agar, 868 IFO Broth, 868 11-() Medium. 802, 869 Ignicoccus islandicus, 869 Ignicoccus Medium, 869 Ignicoccus pacificus, 869 Ignisphaera Medium,. 869 Ignisphaera species, 870 IGP Medium. 878 Ilyobacter Agar, 870 liyobacter Broth. 870 Ilyobacter dclafictdii. 764, 871 liyobacter insuetus, 1883 llyolntcter Medium. 871 Ilyobacter polytropus, 872 Ilyobacter polytropus Medium, 871 Ilyobacter tartaricus, 870,871, 872 Ilyobacter tartaricus Medium. 872 IM, 874 ImhofFs Medium, Modified. 873 Imidazole, 322 Imidazole Utilization Medium, 873 Immunocompromised hosts, 941 In vitro toxigenieity testing, 901, 902 Indole. 837. 1838 Indole Medium, 874 Indole Nitrate HiVeg Medium. 874, 1840 Indole Nitrate Medium, 874 Indole production. 874. 1167. 1191. 1223. 1572,1632.1845.1847 Indole test, 874, 1367,1840 Infant botulism, 398 Infant diarrhea, 1589 Infant formula. 384 Infection Medium, 874

Infusion Agar. 227, 228 Infusion Broth, 875 Infusion Cystine Agar Base, HiVeg. 875 Infusion Cystine Agar Base, HiVeg with 1 Icmoglobin. 875 Inhibitory Mold Agar, 875 Injured coliform microorganisms. 1682 Inonotus hispidus, 775 Inonottts radiants. 348 Inorganic Salt Medium, 876 Inorganic Salts Maltose Medium, 876 Inorganic Salts Starch Agar, 885 Inorganic Salts-Maltose Medium, 875 Inositol Assay Medium, 876 Inositol Assay Medium KB. 876 Inositol Brilliant Green Bile Salts Agar. 877 Inositol Brilliant Green HiVeg Agar, 877 Inositol fermentation, 1388 Inositol Gelatin Deeps. 877 Inositol Urea Caffeic Acid Medium, 877 Inositol utilization, 878 Insect species, 1551 Inspissation, 8 Inter/Hum fxiradoxum. 70 International Streptomyces Project, 884. 885 International Streptomyces Project Medium. 1,885 International Streptomyces Project Medium. 2,885 International Streptomyces Project Medium. 3,885 International Streptomyces Project Medium, 4,885 International Streptomyces Project Medium, 4 with Glucose. 886 International Streptomyces Project Medium, 4 with Yeast Extract, 886 International Streptomyces Project Medium. 5.886 International Streptomyces Project Medium. 6,886 International Streptomyces Project Medium. 7,1862 International Streptomyces Project Medium. 8,1290 International Streptomyces Project Medium, 9,886 Intestinal, 195, 1392, 1811, 1813 Intestinal protozoa. 195 Intestinal treponemes, 1811, 1813 Intracellular Growth Phase Medium, 878 Intracellular iM>ly-|i-hydroxybutyrate production, 1406 Inlrasporangium calvum, 210, 214 Ion Agar for Ureaplasma. 878 Ionagar, 2 Ionic Medium with Pipccolate. 879 Irgasan* Ticaicillin Chlorate Broth, 879 Iron. 880.1005.1657.1662.1785 Iron Agar, Lyngby. 880 Iron bacteria, 323.997,1552, 1553 Iron Bacteria Isolation Medium, 880

Iron Milk Medium. 880 Iron Milk Medium, Modified, 880 Iron Sulfite Agar. 880 Iron Sulfite HiVeg Agar, 881 Iron-Oxidizing Medium. 880 Irradiated I ryptone Soya Broth, 435 Islam GBS Agar. 720 Islams Medium Base for Group B Streptococci, 881 ISM Agar. 881 ISM Broth, 881 Iso Scnsitcst Broth. 882 Isobaculum melis, 440 Isocystis pallida. 884 Isoleucine I lydroxamale Medium, 882 Isonana Medium. 882 Isonema papillatum. 882 Isonema species. 644 Iso-Sensitest Agar, 881 fsosphaera Agar, 882 Isosphaera BroUi, 883 Isosphaera pallida, 883, 884 Isosphaera pallida Medium, 883 IsoVitaleX* Enrichment, 4.884 ISP. 5 Medium.. 885 ISP HiVeg Medium No. 1, 884 ISP HiVeg Medium No. 2, 884 ISP HiVeg Medium No. 6, 884 ISP Medium 1,885 ISP Medium 2, 885 ISP Medium 2 with 5% Sodium Chloride, 885 ISP Medium 3, 885 ISP Medium 4,885 ISP Medium 4 with Glucose, 886 ISP Medium 4 with Yeast Extract. 886 ISP Medium 5, 886 ISP Medium 6,886 ISP Medium 7,1862 ISP Medium 8,1290 ISP Medium 9. 886 ISP2 Medium, 884 ISS1 Medium. 887 Issatchenkia orientalis, 1004 IIC Broth. 879 11C HiVeg Broth Base with 1 icarcillin and Potassium Chlorate, 896 IUT Medium Base wim Glycerol and Egg Emulsion, 887 .1 J Agar. 888 J Broth, 888 Jakoba libera, 1556 Janibacter terrae, 1513 Japonochytrium species, 287 JB Medium with Glucose. 888 JD1 Medium, SS8 JDS Medium, 888 Jensen's Medium, 889 JO. 889 Johnson's Marine Medium, 889

Index

Joiwsia ileiiitrificans. 215 Joncs-Kcndrick Pertussis Transport Medium, 889 Jordan's Tartrate Agar. 889 K K.R.A.NK.P. HiVeg Agar Base with Egg Yolk Kinulsion. 919 K101 Flexibacter Medium, 889 Kado's Agar, 890 Kanagawa reaction. 1899 Kanamycin Fsculin A/ide Agar, 890 Kanamycin Fsculin A/ide Broth, 890 Kanamycin lisculin Azide HiVeg Agar. 890 Kanamycin Fsculin Azide HiVeg Agar Base with Kanamycin, 891 Kanamycin lisculin Azide HiVeg Broth. 891 Kanamycin lisculin Azide HiVeg Broth Base with Kanamycin, 891 Kanamycin L Broth Medium, 891 Kanamycin Luria Agar, 891 Kanamycin Sulfate Selective Supplement, 6 Kanamycin Vancomycin Blood Agar. 892 Kanamycin Vancomycin Lakcd Blood Agar. 892 Karmali's Campylobacter Medium, 308 Kasai Medium, 892 KC Bottom Agar, 892 KC Broth, 893 KC Top Agar, 893 KCN Broth. 893 KDM-2 Medium, 893 Krister's Modified TYI-S-33 Medium, 893 Kelly Medium. Nonselective Modified. 893, Kelly Median, Selective Modified, 894 Kempler-MeKay Agar, 904 Kenknight and Manner's Medium. 895 Kerosene Mineral Salts Medium. 895 Kelogluconale Broth, 895 Kelolactonale Broth, 896 KF Streptococcal Agar Base with Triphyenyltctra/oliuin Chloride, 8% KF Streptococcal HiVeg Agar Base, 896 KF Streptococcal HiVeg Broth Base with BCP and Triphyenyltetrazolium Chloride. 8% KF Streptococcal HiVeg Broth Base with Triphyenyltetrazolium Chloride, 897 KF Streptococcus Agar, 897 KF Streptococcus Broth, 897 KG Agar. 899 KG HiVeg Agar Base. 898 Khawkinea quartana, 631 Kibitelosporangium arulum. 210 Kidney Bean Agar. 898 Kievskaya Agar. 898 Kievskaya Broth. 898 Kievskaya Broth wilh w-1 lexadecane, 898 Kim-Goeptert Agar, 899 Kimmig Fungi HiVeg Agar Base with Cieorge Kimmig Supplement. 899

Kimmig Fungi HiVeg Agar Base with Kimmig Supplement, 899 Kimmig's Agar, 899 Kineococcus nuliotolerans. 1447 Kineosphaera limosa. 1159 Kineosjioiia aurantiaca, 767,768, 1322 King's Medium A, 900 King's Medium B. 900 King's O/F Basal Medium. 900 King's OF HiVeg Medium Base with Carbohydrate, 900 King's OF Medium, 900, 1335 Kirc liner Bmicbment Medium. 901 Kirchncr Medium Base. Modified. 901 Kitasatoa purpurea, 1320 Kitasalospora setae, 768 Kitasatosporia grisea. 886 Kitasatosporia mediocidica, 1318 Kitasatosporia papulosa, 886 K-I. Virulence Agar, 901 Kleb Agar. 902 Klebsiella Medium. 902 Klebsiella oxytoca^M, 1350, 1825, 1831 Klebsiella pneumoniae, 648, 652,903, 1271. 1537.1625 Klebsiella Selective Agar, 903 Klebsiella species, 218,285,493. 494, 839. 903, 1563 Klebs-I .oelller Virulence Agar, 901 Kiebsormidium subtilissimum, 70 Kliglcr Iron Agar. 903 Kligler Iron Agar with Sodium Chloride, 904 Kligler Iron HiVeg Agar, 904 Kligler Iron HiVeg Agar, Modified. 904 Kloeckera apiculata, 877 Kluy\-era species, 1943 Kluyvenmyces lactis, 749, 753,1426,1942 Kluyveromyces marxianus. 1001, 1942 Kiuyveromyces species. 1001. 1004, 1949 KM Agar, 904 Knisely Medium lor Bacillus anthracis, 905 Knoellia sinensis, 1513 Knoellia subterranea. 1513 Koch's Kl Medium, 905 Kohn Two Tube HiVeg Medium No. 1 Base wilh Urea, 905 Kohn Two Tube I liVeg Medium No. 2, 906 Kohn Two Tube Medium No. 1 Base with Urea, 905 Kohn Two Tube Medium No. 2,905 KoKo Medium. 906

Korthof Medium, Modified, 907 Korthof s Medium, 907 Koscr Citrate Broth, 907 Koser Citrate Medium, 907 Kosmachev's Medium, 907 Kozakiu Intliensis, 62 KPL Medium, 907 Kracke Blood Culture HiVeg Medium, 908 Krainsky's Asparagine Agar, 908 KRANEP Agar Base. 908

1993

KRANFP Hi Veg Agar Base with Ivgg Yolk Fmulsion, 908 Kreb's Yeast lactate Medium, 909 Kretzchmaria clavus, 472 Kuehneromyccs mutabiiis. 1065 Kuehniella racovitzae, 706 Kundranl Agar, 909 Kunkee Medium, 909 Kupferberg Trichomonas Base, 909 Kupferberg Trichomonas Broth. 909 Kupferberg Trichomonas HiVeg Broth Base with Scrum and Selective Supplement, 910 Kurthia gibsonii, 454, 1305 Kurthia sibirica. 1429 Kurthia species. 189, 1948 Kurthia zopfii, 454 Kushneria auranlia, 910 Kushneria aurantia Medium. 910 KVBA, 892 KYE Agar. 910

I. L Agar, 911 L and F Basal Salts. Modified with Hcptadccane,912,931 L Broth. 911 I. Broth DAP Thymidine, 911 L Diphasic Blood Agar Medium, 911.912 L forms, 1900 I. Medium, 912 L Medium for Salmonella, 914 I. Medium with Ampicillin, 912 L Medium with DAP and THY. 913 L Medium with DAP. THY, and AMP, 913 I, Medium with Diaminopimelic Acid and Thymidine, 913 I. Medium with Diaminopimelic Acid, Thymidine, and Ampicillin, 913 I. Medium with Methanol, 913 L Medium with Tetracycline. 914 L Salts for 'Ibermophiles, 1186 /,. mono Confirmatory Agar Base, 914 L mono Confirmatory IfiVeg Agar Base. 915 1.. mono Differential HiVeg Agar Base, 915 L15 Medium. Modified l.eibovitz, 914 I .alvl emco, 3 Lab-Lemco Agar. 916 I.ab-I.emcoBrolh, 916 Labrys miyagiemis. 1947 l.ahrys monachus, 330, 1198 Laccaria bicolor. 1199 Laccaria laccala, 1065, 1199 I,achica's Medium, 1526 Lachica's Medium Base. 916 Lachnospira multipara, 616, 617, 1369 Laehnospira muitiparus, 1419. 1522 I.aetalbumin hydrolysate, 2 Lactarius deliciosus. 1325 f.aclarius turpis, 775 Lactate Agar. 916 Lactate Broth, 916

1994

Index

Lactate fermentation, 393 Lactate Sea Water Minimal Medium, 917 Lactic acid. 1231. 1232, 1234. 1235 I .actic acid bacteria, 842,905,917,918,919, 1231 Lactic Acid Bacteria Broth, 917 Lactic Acid Bacteria Medium. 917 Lactic Acid Bacteria Selective Agar Base, 917 Lactic Agar, 917 Lactic Agar for Yogurt Bacteria, Modified. 918 Lactic Bacteria Broth, 918 Lactic Bacteria Differential Agar, 918 Lactic Bacteria Differential HiVcg Agar. 918 Lactic Bacteria Differential HiVeg Broth. 919 Lactic HiVeg Agar. 919 Lactic Streak Agar, 919 Lactic Streak HiVcg Agar. 919 Lactic streptococci, 635, 1484 Lactobacilli, 137, 138,200, 635.636, 748. 918,919,926, 927, 938,970, 986, 1155, 1156,1217. 1329,1330, 1485. 1486. 1487, 1488, 1515. 1516,1517, 1583. 1801,1802 Lactobacilli Agar, AOAC. 919 Lactobacilli Agar, Association of Official Analytical Chemists, 919

Lactobacilli Broth, 636 Lactobacilli Broth, AOAC. 920 Lactobacilli Broth, Association of Official Analytical Chemists. 920 Lactobacilli deMan-Rogosa-Sharpe Broth, 920 I^actobacilli 1 leteroferm Screen 1 liVcg Agar, 1234 Lactobacilli HiVcg Broth. 636. 920 Lactobacilli MRS Broth, 920, 1351 Lactobacilli MRS BroUi with 0.5% Cysteine, 920 Lactobacilli MRS Broth with Cysteine. 920 Lactobacilli MRS Broth wid> I-thanol, 921 Lactobacilli MRS Broth with Mclvalonic Acid, 1351 Lactobacillus, 8664 Medium, 925 Lactobacillus acetotolerans, 257, 1232. 1233,1351 Lactobacillus acidipiscis. 1232 Lactobacillus acidophilus, 749, 1801 Lactobacillus Agar, 2,921 Lactobacillus hackii, 1479 Lactobacillus bifidum. 921 Lactobacillus hifidus Medium, 921 Lactobacillus brevis, 925, 1468 Lactobacillus huchneri, 918 Lactobacillus bulgarictts. 860.922. 941 Lactobacillus bulgaricus Agar, 921 Lactobacillus bulgaricus I liVeg Agar Base with Tomato Juice and Acetate Buffer, 922 Lactobacillus casei. 698, 699. 922, 923

Lactobacillus casei A'lCC 7469,920 Lactobacillus catenaformis, 14 5 9 Lactobacillus Chloramphenicol Agar, 1.922 Lactobacillus Chloramphenicol Broth, 1,922 Lactobacillus Chloramphenicol Medium, 2. 922 Lactobacillus collinoides. 1802 Lactobacillus crispatus, 1459 Lactobacillus delbrueckii. 918,924,1945 Lactobacillus (amentum, 923, 1749, 1945 Lactobacillus fermentum A'I'CC 9338.920 Lactobacillus fructivorans. 156, 909, 921, 924,960,1945 Lactobacillus helveticus. 925 Lactobacillus Hetcrofcrm Screen Agar. 922 Lactobacillus 1 leteroferm Screen Broth, 923 Lactobacillus 1 leteroferm Screen 1 liVeg Broth, 1235 Lactobacillus homohittchii, 156, 921, 923, 924.960 Lactobacillus homohiochii Medium, 923 Lactobacillus iners, 440.441 Lactobacillus jensenii. 924 Lactobacillus kefiranofaciens, 156, 908, 1236 Lactobacillus kefirgranum, 1483, 1484 Lactobacillus kunkcei, 1232 Lactobacillus lactis, 62, 597, 1642 Lactobacillus lactis subspecies cremoris, 62, 597. 905 Lactobacillus lactis subspecies diacetylacfis. 62,597, 905 Lactobacillus leichmamui, 171. 172.465. 1889 Lactobacillus leichmannii A'I'CC 4797, 920 Lactobacillus lindneri. 1351 Lactobacillus malefermentans, 917 Lactobacillus niallaromicus, 1429 Lactobacillus Medium, 923, 924 Lactobacillus Medium II, 924 Lactobacillus Medium III, 924 Lactobacillus Medium IV, 924 Lactobacillus MRS HiVeg Agar, 925, 1234 Ixtctobacilltts MRS HiVeg Broth, 925 Lactobacillus oenos, 949 Lactobacillus Orotic Acid Medium. 925 Lactobacillus panis, 925 Lactobacillus parakefiri. 1483. 1484 Lactobacillus pentosus\ 768 Lactobacillus plantarum, 222. 768, 1288, 1340,1341,1342, 1847 Lactobacillus pontis, 1235 Lactobacillus rimae, 926 Lactobacillus rimae Medium. 925 Ixtctobacilltts rimae Medium with Tween IM , 926 Lactobacillus niminis, 920,1232,1236.1461 Lactobacillus Sake Medium, 924 Lactobacillus salivarius, 923 Lactobacillus sanfrancisco. 990. 1155,1539. 1592 Lactobacillus Selection Agar, 938

Ixtciofnicillus Selection Agar Base, 926 Ixtctohacillus Selection Broth, 938 Ixtetolxtcillus Selection HiVeg Agar Base with Acetic Acid and Polysoibate, 927 Ixtctohacillus Selection HiVcg Broth Base with Acetic Acid and Polysorbate, 927 Ixtctohacillus Selection Oxgall Agar, 927 Ixtctohacillus Selection Oxgall Agar Base, 927 Ixtctohacillus shatj)eae, 921 Ixictobacillus species. 212, 225. 373, 374. 605. 631, 698, 718, 918,920. 921. 923, 924,925.970,984, 1231,1233, 1135. 1236. 1485.1516. 1577.1652.1653. 1801. 1803,1892, 1903 Ixtctohacillus Streptococcus Differential Medium, 970 Ixtctohacillus uli, 926, 1460 IxtctolHicillus vaccinostercus, 1922, 1926 Ixtctohacillus viridescens, 1749 Ixtctolxtcillus viridescens A'ICC 12706. 920 Ixtctohacillus vitulinus, 920, 1232 Ixtctococcus garvieae, 1429 Ixtclococciis lactis, 454, 718, 744, 1429, 1835. 1945 Ixtctococcus lactis subsp. lactis, 987 Ixtctococcus lactis subspecies hordniae, 890 Ixtctococcus piscium,*)2K, 1429, 1932 Ixtctococcus piscium Medium. 927 Ixtctococcus plantarum* 744. 920. 1429, 1835 Ixtctococcus raffinolacfis. 1429. 1933 Ixtctococcus sjiecies, 1903 Lactose Blue Agar. 928 Lactose Blue HiVeg Agar, 280, 928 Lactose Broth. 928 Lactose Casein Ilvdrolysate Medium, 928 Lactose Distillers Solubles Medium. 928 Lactose I-gg Yolk Milk Agar, 928 l.actosc fermentation. 606, 608. 636. 637, 690,817,818,940,950.958,1388,1389, 1524.1557.1625. 1694.1695.1819, 1820. 1918,1919 Lactose fermenting bacteria, 1389 lactose Gelatin Medium, 929 Lactose Gelatin Medium. Modified, 929 Lactose HiVeg Broth. 929 Lactose Lecithin Agar, 929 lactose Medium. 930 Inclose metabolism, 1327 lactose Minimal Medium, 930 Lactose- nonfermenting, 990 Lactose Peptone Agar, 930 lactose Peptone Agar, Double Strength, 930 Lactose Peptone Agar, Half Strength, 930 Lactose Peptone Broth, 930 Lactose Ricinolcate Broth. 930 lactose Sulfite Broth Base, 930 I setose-fermenting bacteria. 495.496. 671. 991

Lambda Broth, 931 Lambda Plates, 931

Index

Lambda Top Agar, 931 I#mprocystis roseopersicina, 1376 Ixunpropedia I valuta. 761 Laiige Medium, 931 Lash Serum Medium. 931 iMsioboiidium orbiculoides, 706 l.asseur Medium. 931 I.amy I Sulfate Broth, 932 Lauryl Sulfate Broth with MUG. 932. 933 I.auryl Sulfate Broth, I'luorocult, 932 Lauryl Sulfate HiVcg Broth. 932 l.auryl Tryptose Broth, 932 Lauryl I'ryptose Broth with MUG, 933 I.auryl Tryptose HiVeg Broth, 932 Lauryl Tryptose Mannitol Broth with Tryptophan, 933 LAVMm2 Medium. 933 LB Agar. 921, 933 LB Broth, Modified, 933 LB Medium. 934 LB Medium for CI 776,936 1 .B Medium for C1776 with Tetracycline and Ampicilhn, 936 LB Medium with lOOmg Kanamycin, 935 LB Medium with 25mg Kanamycin, 935 LB Medium with 50mg of Kanamycin. 935 LB Medium with Ampicilhn. 934 LB Medium with Chloramphenicol, 934 LB Medium with Glucose. 934 LB Medium with IP'l'G Medium, 935 LB Medium with Kanamycin. 935 LB Medium with Rifampicin, 935 LB Medium with Tetracycline. 935 LB Medium with Tetracycline and Ampicilhn. 935,936 LB Medium with Thiamine Monophosphate, 936 LB Medium with Thiamine l>yrophosphate, 936 LB Medium with TMP, 936 LB Medium with TOP, 936 LB Modified Broth. 937 LB Streptomycin Medium, 937 LB Top Agar. 937 LB-L Medium, 938 LBS Oxgall Agar. 927 L B S ' M Agar. 938 LBSâ&#x201E;˘ Broth. 938 LBSâ&#x201E;˘ Oxgall Agar. 927 LC Broth. 938 LCAT Selective Supplement, 6 LI) Agar, 963 LD Bile Agar. 963 IT) Broth, 963 LD Egg Yolk Agar. 964 LD Esculin Agar, 964 LD Esculin HiVcg Agar. 939 LI) Gelatin Agar, 964 LD HiVcg Agar. 939 Ix*ad Acetate Agar, 939 LEB. FDA. 953 Ijxanora cinerea. 951

Lecanora dispersa. 951 Lecanora ruhina, 951

Lecidea species. 951 Lecithin Agar, 939 Lecithin HiVcg Agar, 940 Lecithin Lactose Agar, 940 Lecithin Lipase Anaerobic Agar. 940

Lecithin Tween Medium, 940 Lccithinasc production, 940.964.965.1034. 1035 Lee's HiVcg Agar. 941 Lee's Multidifferential Agar. 941 Lee's Multidifferential HiVeg Agar. 941 Lee's Agar. 941 Legionella Agar Base. 942 Legionella Agar Ivnrichment, 4 Legionella BCYE Growth Supplement, 4 Legionella BMl'A Selective Supplement. 6 Legionella GVPC Selective Supplement, 6 Legionella longbeachae. 471 Legionella Medium, 942 Legionella micdadei. 202 Legionella MWY Selective Supplement, 6 Legionella pneumophila. 201.202.203.204, 205, 234, 235, 281, 288,471, 671, 678, 679.942.1202.1249.1256 Legionella pneumophila Medium, 942 Legionella Selective Agar, 942 Legionella species. 201,202. 203, 204. 205, 234,235,281,332,471, 598,758,942, 943.1154,1202.1256 Legume Extract Agar, 943 Leifson HiVeg Agar. 943 Leifson Medium. 943 I.eifson's Deoxycholale HiVeg Agar, Modified, 943 I.eifsonia xyli sulwp. cynoilontis, 1543, 1548 Leishmania aethiopica. 249 Leishmania amazonensis, 249 Leishmania aristedesi, 249 Leishmania hraziiiemis, 249, 600, 1820 Leishmania chagasi. 249 Leishmania donovani, 249, 600, 912, 944, 1820 Leishmania ennellii. 249, 600 Leishmania garnhami, 249 Leishmania gerhillL 249 Leishmania guyanensis, 249 Leishmania herligi. 249, 944 Leishmania infantum, 249 Leishmania major. 249 Leishmania Medium, 943 Leishmania peruviana. 600 Leishmania pifanoi, 249 Leishmania species, 230. 1300 Leishmania larentola, 600 Leishmania tropica. 249. 600. 944 LI-MB Agar, 950 Lenox Broth. 934 Lentinula edodes, 1571 Lentinus tigrimts. 1065 Lenzites hetulina. 1065

1995

Leomaivs Agar, 944 l.episla inversa, 1004 Leptolinea tardi vitalis. 114 Leptonumas collosoma, 600 Lcptomonas costoris. 600 Leptonumas lactosovorans. 600 Leptomonas mirabilis. 600 Leptonumas pulexsimulantis, 600 Leptomonas pyrrhocoris. 461 Leptonumas samueli, 600 Leptomonas seymouri, 600 Leptospira biflexa. 945 Leptospira horgpetersenii, 945 Leptospira Enrichment, 5 Leptospira HiVeg Medium Base. Fletcher, 684 Leptospira I liVeg Medium Base, Korthof, Modified with Rabbit Serum, 944 Leptospira interrogans, 945 Leptospira Medium. 944 Leptospira Medium, Ellinghausen-McCullouglv' Johnson-Harris, 945 Leptospira Medium. EMJI I, 945 Leptospira Medium, Modified, 945 Leptospira ineyeri. 945 Leptospira noguchii, 945 Leptospira PF Medium. 945 Leptospira Protein-Free Medium, 945 Leptospira sanlarosai. 945 Leptospira species, 239, 240, 241, 684, 907, 944. 945.1477, 1650 Leptospira weili, 945 I.eptospirillum ferrooxidans. 946. 1757 Leptospirdlum ferrooxidans Medium. 946 I.eptospirillum IIII Medium. 946 Leptospirdlum sp. DSM 9468, 1239 I.eptospirillum sj)ecies, 946, 1239 Leptothrix. 2X PYG Medium, 946 Leptothrix cholodnii. 946, 1599 Leptothrix discophora. 946, 1246 Leptothrix discophorous, 1005 Leptothrix mobilis. 1599 Leptothrix ochracea, 946, 1199 Leptothrix ochracea Medium. 946 Leptothrix species, 947,1005, 1599 Leptothrix Strains Medium, 946.947 Leptotrichia buccalis, 693, 694, 892, 947, 1890 Leptotrichia buccalis Medium, 947 Leptotrichia Medium, 947 Letheen Agar, 947 I.etheeii Agar, Modified, 947 Letheen Broth, 948 Letheen Broth. Modified, 948 Letheen HiVeg Agar, 948 Letheen HiVeg Agar, Modified, 948, 1215 Letheen HiVeg Broth. AOAC. 948 Letheen HiVeg Broth, Modified, 948, 1215 Leucogyrophana mollusca. 1004. 1065 Leuconostoc carnosum, 1945 Leuconostoc gelidum. 1945 Leuconostoc laCttS, 718

1996

Index

Leuconostoc Medium, 949 Leuconostoc mesenteroides, 225,488, 718, 949, 1231. 1233,1235, 1652.1944 Leuconostoc oenos, 51, 156, 718, 949 Leuconostoc oenos Medium, 949 Leuconostoc species, 22, 51, 748, 857, 920, 1686.1801. 1944 Leucosporidium scottii, 1878 Leucofhrix Medium, 949.950 Leucothrix mticor, 950, 1337, 1672 Leucothn'x mttcor Medium. 950 Leucothrix species. 949, 1438 Levari production, 1652 Levilinea saccharolytica. 113 Levine EMB Agar, 950 Lcvinc Eosin Methylene Blue Agar. 950 Levinlhal's HiVeg Medium Base with Blood, 950 Levinlhal's Agar, 950 LcvinthaFs Medium Base. 950 L-fotms, 288 LGI Medium, 164 LIIKT2 Medium, 951 LHKT2 Medium with Yeast Extract or Yeast Autolysate, 951 Lichen Fungi Medium, 951 LICNR Broth. 951 Lignincola laevis. 287 Lima Bean Agar, 952 Limnohacter Medium, 952 Limnobacter thiooxidans, 952 I .mdanc Medium, 952 Lindehna macrospora, 944 Lindra thalassiae, 287 Lincoln Agar, 952 Lingulamoeba leei, 1011 Lipase activity. 398.1571 Lipase production, 940,965,1034. 1035 Lipolytic fungi, 1814 Lipolytic microorganisms, 1573,1574, 1815 Lipomyces kononenkoae. 749 Lipomyces starkeyi, 584 Lipovitellin Salt Mannilol Agar. 952 Liquid fermentation, 1448. 1449 Liquid media, 1792 Liquid specimens, 291.292.1943 I.iquoid Broth, 953 Listeria denitriftatns, 215 Listeria Fnrichment Broth, 953 Listeria Fnrichment Broth I. USDA 1'SIS. 953 Listeria Enrichment Broth II. USDA FSIS. 954 Listeria Enrichment Broth, FDA, 953 Listeria Fnrichment HiVeg Agar. 954 Listeria Fnrichment HiVeg Broth, 954 Listeria Fnrichment HiVcg Broth. Modified. 955 Listeria Enrichment HiVeg Medium Base with Acriflavine and Nalidixic Acid, 955 Listeria Fermentation Broth, 955 Listeria 1 liCrome Agar Base. Modified. 839

Listeria Identification 1 li Veg Agar Base with PALCAM Selective Supplement, 955 Listeria Identification HiVeg Broth Base with PALCAM Selective Supplement, 956 Listeria ivanovii, 385 Listeria monocytogenes, 218, 222.227, 283, 385, 649, 705, 706, 720, 839, 851, 915, 953,954,955,956,957,969,1032,1033, 1215.1217,1225,1334. 1335,1340, 1830, 1834, 1876 Usteria monocytogenes Confirmatory Agar, Base, 914 Listeria Motility HiVeg Medium. 956 Usteria Motility Medium. 956 Listeria Oxford HiVeg Medium Base with Antibiotic Inhibitor. 956 Listeria Oxford Medium Base with Antibiotic Inhibitor. 956 listeria Primary Selective Enrichment Broth, UVM 1.953 Usteria Primary Selective Fnrichment Broth, UVM II, 954 Listeria Primary Selective Fnrichment Sujvplement, 6 Usteria Selective Agar, Modified Oxford. 1216, 1334 Usteria Selective Agar, Oxford, 1334 Listeria Selective Enrichment Supplement, 6 Usteria Selective I li Veg Agar. 957 Usteria Selective HiVeg Broth Base wim Antibiotic Inhibitor, 957 Usteria Selective Supplement MOX, 7 Listeria Selective Supplement Oxford, 7 Listeria species, 86, 87,215, 282, 493, 646, 704.705.775.839.955.956.957.958. 1277, 1340. 1449, 1481. 1820 Listeria Transport Enrichment Medium, 957 Ustoneila angudlara, 1617 Listoneila anguillarum, 644, 1833 LIT Medium, 958 I ithium Chloride Phenylethanol Moxalactam Plating Agar, 968 Lithium Phenylethanol Moxalactam Agar, 969 Litmus I-actose Agar, 958 Litmus Lactose Agar with Crystal Violet. 958 Litmus l-actose HiVeg Agar, 958 Litmus Milk, 958 I.ittman Oxgall Agar, 958 Littman Oxgall I IiVeg Agar Base with Streptomycin. 959

Liver Broth, 959 Liver Broth with NaCl. 960 Liver digest neutralized, 2 Liver Infusion Agar, 960 Liver Infusion Agar, HiVeg. 960 Liver Infusion Broth. 960 Liver Infusion Broth, HiVcg, 960 Liver Infusion Sake Medium, 960 Liver Meat Infusion HiVeg Agar, 960 Liver Veal Agar, 961

Liver Veal Egg Yolk Agar. 961 LL Agar, 958 ELK Agar. 958 h)homonas piriformis, 70 lx>bosphaera Medium, 961 Ix>hosphaera species, 962 lAxlobacterflitvialilis, 215 LoeferfS Medium, 962 Ixrcfflcr Blood Scrum Medium. 962 LoefiDer HiVeg Medium Base with Asolectin. 962 Ijocffler Medium. 962

Loeffler Slant, 963 lx>cfflcr Slant. Modified, 963 LOM Agar. 977 I.ombard-Dowell Agar, 963 I-ombard-Dowell Agar, HiVeg. 939 lxwnbard Dowell Bile Agar, 963 I.ombard-Dowell Broth, 963 Ix>mbard-Dowell Egg Yolk Agar. 964 I jombard-Dowell Esculin Agar, 964 l.ombard-Dowell Esculin Agar, HiVeg. 939 Ix>mbard-Dowell Gelatin Agar, 964 I-ombard-Dowcll Neomycin Agar, 965 I-ong-Term Preservation Medium, 965 bophotriehus incaniatus, 1476 Ix>w Iron YC Agar, 965 IJOW Iron YC Broth. 966 Ijow Phosphate Buffered Basal Medium, Modified. 966 Lowenstem-Gmfl Medium, 967 Ixnvenstein-Jensen Medium, 967, 968 l.owcnstein-Jensen Medium wim Sodium Chloride, 967 Ix>wcnstcin-Jcnscn Medium without Glycerol, 968 LPBM Acido-Thcrmophilc Medium, 968 LPM Agar, 968, 969 LPM Agar with Plsculin and Ferric Iron, 969 LPM I IiVeg Agar Base with Moxalactam, 969 IS Differential HiVeg Medium Base with Skim, Milk and Triphenylietra/olium Chloride. 970 LS Differential Medium. 970 LST-MUG,933 LST-MUQ Broth, 970 LT Medium. 940 L'I'H Medium for Thiolhrix, 970 LTH Medium for Thiolhrix with Casitone, 971 Lucibacterium harveyi, 1558 Lucibacterium species. 753 I.uedemann Medium, 971 Lulworthia medusa, 639 Lulworthia species, 638 Luminous Medium. 971 LUP.971 EuPhcil Medium. 972 Luria Bertani HiVeg Agar, Miller, 973,1168 Luria Bertani HiVeg Broth, Miller, 973.1168 Luria Broth, 934

Index

Luria Broth. HiVeg, 973 I.uria HiVeg Agar. 973 l.uria HiVcg Agar with Glucose. 973 Luria-Bertani Medium, 934 LuTria3 Medium, 973 LY Agar lor Piiobasidium, 974 Lycoperdonfoetidum, 1065 Lyngby Iron Agar, 880 Lyophyllumfumosum, 1199 l.yophyllwn sbimeji, 1199 Lysatc production. 1039.1472 Lysine Agar. Selective. 974 Lysine Arginine Iron Agar, 975 Lysine Arginine Iron HiVeg Agar, 975 Lysine Broth Falkow with Sodium Chloride, 975 Lysine deaminase. 1167, 1223 Lysine decarboxylase. 975.976,977. 1167, 1223, 1514,1520 Lysine Decarboxylase Broth, Falkow, 975 Lysine Decarboxylase Broth. Taylor Modification, 975 Lysine Decarboxylase HiVcg Broth, 976 Lysine Decarboxylase Medium, 976 Lysine Iron Aga. 976 Lysine Iron Cystine I liVeg Broth Base with Novobiocin, 976 Lysine Iron Cystine Neutral Red Broth, 951 Lysine Iron HiVeg Agar, 976 Lysine Lactose HiVcg Broth, 977 Lysine Medium, 977 Lysine Ornithine Mannilol Agar, 977 Lysine-decarboxylase. 1918. 1919 Lysobacter antihioticus, 449 Lysobacter bntnescens, 449 Lysobacter deserti, 978 Lysobacter deserti Medium. 977 Lysobacter enzymogenes, 449, 1926, 1931, 1936 Lysobacter gummosus, 449 Lysobacter species. 449.456 Lysozyme Broth, 978 Lysozyme sensitivity. 978 M

M 40 Y, 1332 M 40 Y Agar. 988 M Broth, 978 M Medium. 978 Ml Medium, 979 M10 Broth, 984 Ml3 Verrucomicrobium Medium. 984 Ml4 Medium, 985 M16 Agar. 985 M16 HiVcg Agar, 1217 Ml 7 Agar. 986 Ml7 Broth, 986 MI7 HiVcg Agar Base with Dis<xIium-(5glycerophosphate, 986 MI7 Medium for Filomicrobium fusiforme, 987 Ml7 Medium for I-actic Streptococci, 987

M17 Medium. Modified, 987 MIA Medium, 979 Ml-Nocardiopsis arabia Medium.. 979 M3 Agar. 980 M3 Agar Medium, 980 M3 Chytrid Agar. 980 M56 Agar, 988 M56 Medium, 988 M63 Medium. 5X. 988 M7 Medium. 980

M9 Medium. 981 M9 Medium with Arginine, 981,982 M9 Medium with Casamino Acids. 982 M9 Medium with Tryptophan, 983 MA Medium, 989 MAI, 999 MA2, 999 MA2 Willi Lupine Stems, 999 MA4, 999 MA4 with Lupine Stems, 999 MA8. 999 MAB1 Medium, 989 MACA with Maltose. 990 MacConkey Agar, 990 MacConkcy Agar Base. HiVeg, 990 MacConkey Agar No. 2.991,992 MacConkcy Agar No. 3.992 MacConkey Agar with 0.15% Bile Salts, Crystal Violet, and Sodium Chloride. HiVcg. 991 MacConkey Agar with Sorbitol, 1590 MacConkey Agar wiiliout Crystal Violet. 991 MacConkey Agar without Crystal Violet and Sodium Chloride with 0 .5% Sodium Taurocholate. HiVeg. 992 MacConkey Agar without Crystal Violet with Sodium Chloride and 0.5% Sodium Taurocholate, HiVeg, 991 MacConkcy Agar without Salt. 992 MacConkey Agar, CS, 991 MacConkey Broth, 992 MacConkey Broth. lHirple, 993 MacConkey Broth. Purple, wilh Bromcresol Purple," HiVcg, 993 MacConkey I liVeg Agar with Bromlhymol Blue. 993 MacConkey HiVeg Agar with Crystal Violet and Sodium Chloride. 993 MacConkey HiVeg Agar without Crystal Violet and Sodium Chloride. 993 MacConkey HiVeg Agar, Modified. 994 MacConkey HiVeg Broth (Double Strength) with Neutral Red. 994 MacConkey I liVeg Broth Purple with Bromo Crcsol Purple, 994 MacConkey II Agar. 992 MacConkey ltiosphatase Medium. 1037 MacConkey Sorbitol HiVeg Agar, 994 MacConkey Sorbitol Agar. HiCromc. 839 Macrolepiota procera, 1065 Macrolepiota rhacodes, 1065. 1199 Xlacronodus bifurcatus. 706

1997

Macrospore, 1321 M-Aeromonas Selective Agar Base. Havelaar,994 Magnesium Oxalate Agar, 994 Magnetic Spirillum Growth Medium. Revised, 995 Magnetospirillum, 2 Medium. 996 Magnetospirillum gryphiswaklense, 996.997 Magnetospirillum magnetotacticum, 996, 997 Magnetospirillum Medium, 995 Magnetospirillum Semi-solid Medium. 996 Maintenance HiVeg Medium for R. subtilis ATCC 6633.997 Maintenance of I. Antigen in Neisseria, 997 Maintenance SCY Medium. 1552 Malachite Green Broth, 997 Malassezia furfur, 1400 Malassezia pachydermatis. 1400 Malassezia species, 602, 1531 Maleate Medium for Pseudomonas fluoresces, 997 Maleate Medium for Pseudomonas fluoresces with Glucose and Phenol Red, 997 Malonate Broth, 998 Malonate Broth, Lwing Modified, 998 Malonate utilization. 1393 Malonate-uiili/ing bacteria, 998 Malonomonas rubra. 506. 509

Malt 2% Yeast Lxtract Agar, 1004 Malt 4% Dextrose Peptone Yeast Agar. 1000 Malt 4% Dextrose Yeast Agar, 1000 Malt 4% Yeast Extract Agar. 1004 Malt 4% Yeast Lxtract Agar with Lupine Stems. 1004 Malt 8% Yeast Lxtract Agar, 1004 Malt Agar, 998 Malt Agar 1%. 998. 999 Malt Agar 2%, 999 Malt Agar 2% with Lupine Stems. 999 Malt Agar 4%, 999 Malt Agar 4% with Lupine Stems. 999 Malt Agar 8%, 999 Malt Agar with 0.5% CaC03.999 Malt Agar with 2% Malt, 999 Malt Agar. 1/3 Strength, 998. 1312 Malt Agar, Blakeslee, 99S Malt and Peptone Medium. 1003 Malt Dextrose 40% Peptone Agar, 1000 Malt IXwtrose Peptone Agar, 1000 Malt extract, 4

Malt Lxtract Agar. 1000. 1001 Malt Lxuact Agar for Yeasts and Molds, 1002 Malt Lxtract Agar. Blakeslee's, 1001 Malt Lxtract Agar. Half Strength, 1001 Malt Lxtract Broth. 1002 Malt Extract Charcoal Medium, 1002 Malt Lxtract Glucose Agar. 1002 Malt Extract HiVeg Agar Base, 1002 Malt Extract HiVeg Agar Base. Modified. 1002

1998

Index

Malt Kxtract HiVeg Broth Base. 1003 Malt 1-xtract Peptone Agar, 1003 Malt Extract Yeast Kxtracl 40% Glucose Agar, 1003 Malt Peptone Yeast Kxtracl Agar. 1230 Malt Yeast Agar, 1003 Malt Yeast 1-xtract 50% Glucose Agar, 1312 Maltea. 1004 Maltose fermentation. 1389 Maltose fermenting bacteria, 1514, 1520 Maltose Peptone Yeast 1-xtract Broth. 1230 Maltose Peptone Yeast 1-xtract Medium. 1230 Mammalian cells, 1520.1794. 1795 Mammalian expression vector. 1696 Manganese Acetate Agar, 1004 Manganese Agar No. 1, 1004 Manganese Agar No. 2. 1005 Manganese Medium for Pseudomonas species, 1005 Manganese Nutrient Agar. 1005 Manganese-oxidizing bacteria. 1004 Manning Medium, 1005 Mannitol Agar, 1005 Mannitol Agar with IVilion, 1005 Mannitol Kgg Yolk Polymyxin Agar, 1006 Mannitol fermentation. 335, 388,433. 434. 655, 673, 953, 977, 1390, 1845. 1846 Mannitol Lysine Crystal Violet Brilliant Green Agar. 1196 Mannitol Maltose Agar, 1006 Mannitol Motility Test HiVcg Medium. 1006 Mannitol Salt Agar, 1006 Mannitol Salt Agar with Kgg Yolk Kmulsion, 1007 Mannitol Salt Broth, 1007 Mannitol Salt HiVcg Agar Base. 1007 Mannitol Salt HiVcg Broth, 1007 Mannitol Selenite Broth, 1007 Mannitol Selenite Broth with Brilliant Green, 1008 Mannitol Yeast Kxtracl Medium, 1008 Mannitol Yolk Polymyxin Agar, 1008 Mannitol-utilizing, 1006, 1007 Mannitol-Yeast Kxtract-Pcptonc, 1008 Maricaulis Medium, 1009 Maricaulis species, 1619 Marichromatium bheemlicum. 1496 Marine. 152.330.337.388.634.1010.1011, 1014, 1041, 1056. 1096, 1438, 1605.

1669 Marine Agar, 1009 Mamie Agar, 2216. 1009 Marine Agar with Biphcnyl. 1010 Marine Agar with i-Carrageenan, 1010 Marine Agar with k- and 1-Carrageenan, 1010 Marine Agar with Naphthalene. 1010 Marine Agar with Sulfur. 1011 Marine Ameba Medium, 1011 Marine ammonia-oxidizing bacteria, 1041 Marine bacteria, 889,1018,1019.1555.1557

Marine Broth, 2216, 1011 Marine Broth with Biphenyl, 1011 Marine Broth with i-Carrageenan. 1012 Marine Broth with k- and 1-Carrageenan, 1012 Marine Broth with Naphthalene, 1012 Marine Broth with Sulfur. 1013 Marine Cauhhacter Medium, 1013 Marine Chlorobiaccac Medium. 2. 1013 Marine Chromatiaceae Medium, 2, 1014 Marine cyanobaclcria. 1200 Marine Cytophaga Agar. 1014 Marine Cytophaga Medium, 1014 Marine Cytophaga Medium A, 1015 Marine Cytophaga Medium B. 1015 Marine Cytophaga Medium C, 1015 Marine Desulfovihrio Medium, 1015 Marine enterococci, 634 Marine Flagellate Medium. 1015 Marine l-'lagellate Medium With B- Vitamins. 1015 Marine Glucose Trypticase1M Yeast Kxiract Agar, 1016 Marine Glucose 1 rypticascIM Yeast Kxtracl Broth, 1016 Marine habitats. 152.388. 1438. 1605 Marine isolates, 330 Marine Methanogenium Alcohol Medium. 1016 Marine Methanol Medium, 1017 Marine Mcthylotroph Agar, 1017 Marine Methylotroph Broth, 1017 Marine mud. 1096 Marine nitrate-oxidizing bacteria, 1056 Marine Oxidation Kermcntation HiVcg Medium, 1018 Marine Peptone Succinate Salts Medium. 1018 Marine Peptone Yeasi Medium with Magnesium Sulfate, 1018 Marine Pseudomonas Medium, 1018 Marine purple sulfur bacteria. 1375 Marine Rhodococcus Medium, 1018 Marine Rhodopsettdomonas Medium. 1019 Marine Salts Medium, 1019 Marine Salts Medium for Sporosarcina halophila, 1019 Marine Spirochete Medium. 1020 Marine Spirochete Medium,, 1019 Marine Tiiermocaccus Medium. 1020, 1021. 1022 MahnUahilia salmanicolor, 477 Marinithermus hydrothermalis, 1022 Marinithermus hydrolhermalis Medium, 1022 Marinitoga camini, 1023 Marinitoga Medium, 1022 Murinitoga piezophda, 1023, 1024 Marinitoga piezophda Medium. 1023 Marinobacter hydrocarbonoclasticus, 1009 Marinobacter iutaoensis, 1024 Marinoimcter lutaoensis Medium, 1024

Marinobacter Medium. 1024 Marinobacter species, 1024 Marinobacterium /annaschii, 1558 Marinococcus albus, 1024, 1025, 1200, 1471. 1554 Marinococcus albus Agar, 1024 Marinococcus albus Medium. 1024 Marinococcus communis, 1343 Marinococcus halophdus. T9S. 1200. 1471. 1537, 1538,1554 MarinociKcus hispanicus, 1200 Marinomonas communis. 1025 Marinomonas vaga, 1025 Marinomonas vaga Medium. 1025 Marinospirillum minutulum, 1009 Marionobacter species, 1024 Martelella mediterranea. 1941 Martin-l^wis Agar, 1025 Martin-Ix'wis Agar, Enriched, 1025 Massisteria marina. 1556 Mastigamoeba invertens, 831 Mathanosarcina mazei, 1124 Mating Agar, 1026 Maximum Recovery Diluent. 1026 M-Azide HiVeg Broth Base with Triphenyltclrazolium Chloride. 1026 MB Medium. 1026,1027, 1028, 1029 M-BCG Yeast and Mold Agar. 1030 M-Bismuth Sulfite Broth. 1030 M-Bismuth Sulfite Broth. 224 M-Bismuth Sulfite HiVeg Broth, 1030 MBM Acelate Medium, 1031 MBM Medium, 1139 MBM Medium (Modified), 1031 M-Brilliant Green Broth, 1031 M-Brilliant Green Broth, 262 M-Brilliant Green HiVeg Broth. 1031 M-Broth, HiVeg, 1032 MCA, 1032 McBride Agar, Modified, 1032 McBride Usteria Agar, 1032 McBride Usteria HiVeg Agar Base with Blood and Selective Supplement. 1032 McBride Usteria HiVeg Agar Base, Modified, with Blood and Selective Supplement. 1033 McCarthy Agar, 1033 McClung Carbon-Krcc Broth. 1033 McClung Toabe Agar, 1033 McClung Toabe HiVeg Agar Base wtih Kgg Yolk, 1035 McClung-'l oabc Agar, Modified. 1034 McClung-1'oabc Kgg Yolk Agar, CDC Modified. 1035 McCoy cells, 621 MCM. 438 M-Coliform HiVeg Broth. 1067 M-CP Agar Base, 1035 M-CP HiCromcâ&#x201E;˘ Agar Base. 840 M-CP HiVeg Agar Base with Phenolphthalein Diphosphate, 1036 MCP Medium, 1037

Index

M-CP Medium. 1036.1065 MI) 1 Medium, 1037 MD Medium, 1037 MD1 Medium,, 1037 M~Dextrose Tryptone Broth, 588 MDPA, 1000 MDPA, 40. 1000 MDPA with Calcium Carbonate, 1038 MDPYA4. 1000 MDYA4, 1000 M-H Agar. 615 Me 15% Mil Agar. 1038 Me 15% Mil Medium, 1038 MI". A, 1()00, 1001 Meat. 137.138.623 Meat Extract with Peptone, 1038 Meat Extract with Peptone and 1.5% Sail, 1039 Meat Infusion Agar, HiVeg, 1039 Meat products. 263. 1627 MFAYM, 1002 M-EC Test Agar. 1039 Mechercharimyces mesophilus, 1688 MED Ha, 1039 Media Fill Trials, 436 Medium 2 A. 1040 Medium 2508-85-1 with Amino Acids. 1040 Medium 4 m 1,1053 Medium 523M, 1054 Medium 9K, 1289 Medium A, 1039 Medium A for Producing Lysales, 1039 Medium AS4. 1040 Medium B for Sulfate Reducers, 1042 Medium BG 11 for Cyanobactcria. 212.213 Medium BG 11 for Marine Cyanobacteria, 213 Medium C for Sulfate Reducers. 1043 Medium D, 325 Medium D for Sulfate Reducers, 1046 Medium D for Thermus, 1046 Medium D for Thermits, Modified, 1046 Medium D. Modified. 326 Medium D2, 1047 Medium 1M, 1047 Medium DG, 326 Medium DGN. 326 Medium B for Bacillus, 1048 Medium E for Sulfate Reducers, 1048 Medium E-2, 1048 Medium F, 1050 Medium F for Sulfate Reducers. 1050 Medium for Acetivibrio cellulolyticus, 199 Medium for Aciduric. Thermophilic Bacillus Strains. 1040 Medium for Ammonia Oxidizers. 1040,1041 Medium for Ammonia Oxidizers, Brackish, 1041 Medium for Ammonia Oxidizers, Marine, 1041 Medium for Ammonia-Oxidizing Bacteria, 1041

Medium for Bacillus schlegelii. 1042 Medium for Bacillus stearothennophilus, 1042 Medium for Campylobacter DSM 806, 1043 Medium for Carbon Monoxide Oxidizers. 1043,1044 Medium for Chlorate Respirers. 1044 Medium for Chlorobium ferrooxidans, 1045 Medium for DSM 14457. 1048. 1159 Medium for DSM 14458. 1047, 1048 Medium for Ectothiorhodospira, 10*9 Medium for Ervthrobacter longus, 1050 Medium for Freshwater Flexibacteria, 1051 Medium for Halophilic Archaea. 1052 Medium for Halophilic Bacilli. 1053 Medium for Hydrocarbon-Degrading Bacteria, 1053 Medium for Lactobacilli, 1053 Medium for Marine Flexibacteria, 1054 Medium for Marine Mcthylotrophs. 1054 Medium for Nitrite Oxidizers, 1055 Medium for Nitrite Oxidizers, Marine, 1056 Medium for Osmophilic Fungi, 1056 Medium for Prosthecomicrobium and Ancalomicrohium, 1057 Medium for Prosthecomicrobium and Ancalomicrobium wim Nicotinamide, 1058 Medium for Prosthecomicrobium and Ancalomicrobium. Modified. 1058 Medium for Roseospira, 1059 Medium for Sulfate Reducers. 1061. 1062 Medium for Thermophilic Actinomycetes, 1063 Medium for Treponema pectinovorum, 1063 Medium for Ureaplasma, 170, 171 Medium G for Sulfate Reducers, 1051 Medium K. 1053 Medium K, 1053 Medium M71, 1054 Medium N. 1055 Medium N for Sulfate Reducers, 1055 Medium ND, 327 Medium R. 1059 Medium S. 1060 Medium SP 4, 1060 Medium VTY. 1063 Medium will) Biphenyl, 1043 Medium wim Chloroacrylic Acid, 1045 Medium with EDTA as Carbon and Nitrogen Source. 1049. 1072 Medium with EDTA as Caibon Source, 1049 Medium with Fluoranthcnc, 1051 Medium with Phcnanthrenc, 1056 Medium with Polyhydroxybulyric Acid as Carbon Source. 1056 Megamonas hypermegas, 366, 1459 Megasphaera cerevisiae. 631. 1369.1453. 1456,1459 Megasphaera elsdeni, 1363 Megasphaera elsdenii, 6M, 1064. 1419. 1456.1459 Megasphaera Medium. 1064

1999

Mchlman's Maintenance HiVeg Medium, 1064 Meiothermus taiwanensis, 325. 327 Melanine production, 1863 Melanospora tiffanii. 1952 Melin-Norkrans Medium, 1064 Melissococcus pinion, 1065, 1405 Melissococcus pluton Medium, 1065 Melissococcus plutonius, 1065 MelisstK'occus plutonius Agar, 1065 Melittangium lichenicoia, 1594 Meltisococcus pluton, 1065 Mellisococcus pluton Medium. 1065 MFM with llarle's Salts and Nonessential Amino Acids. 621 Membrane Clostridium perfringens HiCromcâ&#x201E;˘ Agar Base. 840 Membrane Clostridium perfringens Medium. 106S Membrane filter analysis, 1472.1473 Membrane Filter method, 224,262,263,290, 291.391.615.634.641.642.649.650. 857,903.932. 1030. 1066, 1067, 1069, 1167, 1196,1242, 1338, 1404, 1440, 1514. 1515.1567.1577.1618.1631. 1633. 1634, 1682, 1690. 1700. 1704 Membrane I .actose Glucuronidc Agar, 1066 Membrane I .auryl Sulfate Broth, 1066 M-Endo Agar. LES. 641 M-Kndo Broth. 642 M-Endo HiVeg Agar FES, 1066 M-Endo HiVeg Broth, 1066 M-EDdO HiVeg Broth MF, 1067 Meningococci. 244. 1851 Meniscus glaucopis, 1067, 1068 Meniscus glaucopis Agar. 1067 Meniscus glaucopis Broth, 1067 M-Enrichmcnt Brodi. 1068 M-Enleroc-occus Agar, 649 M-Enterococcus Agar Base with Polysorbate, 80 and Sodium Carbonate. 1068 M-Enterococcus Agar Base. Mixlilicd. 1068 M-Enlerococcus HiVeg Agar Base, 1068 Mercurial preservatives, 1773 MeReSa Agar Base wnh Methicillin, 1069 MeReSa Agar Base with Oxacillin. 1069 MBS A p r , 1864 Mesanophrys chesapeakensis, 989 Mesotaenium caldariorum, 70 Metal Acetate Agar. 1069 Metal Acetate Broth, 1070 Metal ACdate Yeast Broth with Arginine, 1071 Melallogenium Cultivation Broth, 1072 Metallogenium Isolation Agar. 1072 Melallogenium Medium. 1072 Metallogenium species. 1072 Metallosphaera Medium, 1072 Metallosphaera scdula. 1073 Metals "44", 5 Metanophiys diminuta, 989 Metanophrys species. 226

20()0

Index

Melarhizium anisopliae, 1410 Metarhizium jlavaviride, 1410 Methanobacillus Medium, 1073 Methanobacillus species. 1073 Methanobacter species, 1136, 1137 Methanobacteria Medium, 1073 Methanobacteria Medium with Glucose and Yeast Kxtracl, 1074 Methanobacteria Medium with Xylose, Yeast Kxtract and Tryptone, 1074 Methanobacteria Medium with Yeast hxtract, Sodium Acetate and Methanol. 1075 Methanobacterium akaliphilum, 1076. 1578 Methanobacleriunt alcaliphilum Medium. 1076 Methanobacterium bryantii, 1084 Methanobacterium hnrichment Medium. 1076 Methanobacterium espanolae. 1077 Methanobacterium espanolae Medium, 1077 Methanobacterium formicicum. 1074. 1077. 1084, 1085 Methanobacterium formicicum Medium. 1077 Methanobacterium II Medium. 1083. 1084, 1085 Methanobacterium Mass Culturing Medium. 1077 Methanobacterium Medium, 1078 Methanobacterium oryzae, 1084.1086 Methanobacterium ruminantium, 1079 Methanobacterium ruminantium Medium. 1077,1078 Methanobacterium species. 1076, 1078. 1082,1083, 1104 Methanobacterium sublenvneum. 1079 Methanobacterium subterraneum Medium, 1079.1736 Methanobacterium thermoalcalip, 1578 Methanobacterium thennoautolrophicum, 1074,1080, 1081. 1082. 1240 Methanobacterium thermoauiotrophicum Marburg Medium, 1079 Methanobacterium thennoautolrophicum Medium, 407. 1080 Methanobacterium thennoautolrophicum Medium, Taylor and Pirt, 1081 Methanobacterium thennoautolrophicum MS Medium. 1081 Methanobacterium thennoaulolrophicus. 1081 Methanobacterium Transduction Agar. 1082 Methanobacterium Transduction Medium, 1082 Methanobacterium uliginosum. 1578 Methanobacterium wolfei. 1083. 1240 Methanobacterium wolfei Medium, 1083 Methanobrevibacter arboriphilicus. 1074 Methanobrevibacter curvalus, 1087. 1089 Methanobrevibacter curvalus DSM 11111. 1088

Methanobrevibacter curvalus Medium. 1086. 1087, 1088 Methanobrevibacter ruminantium. 1578 Methanobrevibacter smilhii, 1240 Methanocalculus halotolerans. 1089 Methanocaladus halotolerans Medium, 1089 Methunocalculus pumilus, 1090 Methanocalculus pumilus Medium, 1089 Methanocalculus taiwanensis, 1027 Methanocalculus taiwanensis DSM 14648. 1029 Methanocalculus taiwanensis DSM 14663, 1030 Melhanococcoides Medium, 1090 Methanococcoides methylutens. 1091. 1119 Melhanococcoides species, 1098 Methanococcus deltae. 734. 1091 Melhunococcus deltae Medium, 1091 Methanococcus frisius. 1104 Melhaiu}c<x:cm jannaschii, 1092 Methanococcus jannaschii Medium, 1091. 1092 Methanococcus maripaludis, 1104 Methanococcus mazei. 710. 1084. 1085 Methanococcus McC Medium, 1092 Methanococcus McN Medium. 1093 Methanococcui McNail Medium, 1094 Methanococcus Medium. 1095 Methanococcus species, 1096 Methanococcus sp. Medium. 1095 Methanococcus species, 1092, 1093, 1094, 1095.1104 Methanococcus thermolifhotrophicus, 1104 Methanococcus vannielii. 734, 1096, 1097 Methanococcus vannielii Medium. 1096 Methanococcus wltae, 734.1027. 1097. 1913 Methanococcus vtdlae BD Medium, 1097 Methanococcus vollae, 1027 Methanocorpusculum aggregans, 1100, 1101 Methanocorpusculum Medium. 1097 Melhunocorpusculum species, 1104 Methanoculleus bourgensis, 1102 MethaiUK'ulleus marisnigri, 1105 Methanoculleus oldenburgensis. 1081 Methanoculleus olentangyi, 1099, 1105 Methanoculleus olentangyi Medium. 1098 Methanoculleus species, 1104 Melhanofollis aquaemaris, 1027 Mefhanqfollis aquaemaris DSM 14661, 1028 Mcthanogen Hnrichmcnt Medium. Barker, 1099 Methanogen Medium. 1099 Mcthanogen Medium, Xeikus. 1099 Methanogenic bacteria, 1099, 1100 Methanogenium aggregans, 1100 Melhanogenium aggregate Medium. 1100 Methanogenium Alcohol Medium. 1101 Melhanogenium Ixjurgense, 1102, 1103, 1240 Methanogenium Ixntrgense Medium. 1102

Methanogenium cariaci, 734 Melhanogenium CV Medium. 1103 Melhanogenium Medium. 1103. 1104 Melhanogenium olentangyi, 734, 1105 Melhanogenium olentangyi Medium. 1104 Methanogenium organophilum. 1103 Methanogenium species. 1017,1102, 1104. 1105, 1240 Methanogenium species Medium, 1105 Methanogenium thennophilicum, 1106 Methanogenium thennophilicum Medium 1105 Melhanogeniummarisnigri, 734 Methanohalobium evestigalus. 1107 Methanohalobium Medium, 1106 Melhanohalobium species, 1152 Methanohalophilus euhalobius, 1107 Methanohalophilus euhalobius Medium. 1107 Methanohalophilus halophilus, 1108.1152 Methanohalophilus halophilus Medium, 1107 Methanohalophilus mahii. 795, 1109 Methanohalophilus mahii Medium, 1108 Methanohalophilus Medium. 794 Methanohalophilus oregonense, 526, 1109 Methanohalophilus oregonense Medium. 1109 Methanohalophilus species. 1152, 1153, 1568 Methanohalophilus zhilinae. 78. 1110 Methanohalophilus zhdinae Mediiun, 1109 Methanol Agar. 1110 Methanol Ammonium Salts Medium. 1110 Methanol Medium. 1111 Methanol Medium Tor Achromobacler, 1111 Methanol Medium with 1% Peptone, 1111 Methanol Mineral Salts Medium, 1111 Methanol Salts Medium. 1111 Methanol Urea Mineral Salts Medium. 1112 Methanol utilization. 1924 Methanolobus. 2 Medium, 1114 Melhanolobus bombayensis, 1098 Methanolobus Medium. 1113 Methanolobus siciliae, 1114 Methanolobus taylorii, 1115 Methanolobus taylorii Medium, 1114 Melhanolobus tindarius, 1114 Methanolobus vulcani, 1114 Methanol-Utili/ing Bacteria Medium B. 1112 Methanol-Utili/ing Bacteria Medium D, 1112,1113 Methanol-Utili/ing Bacteria Medium E, 1113 Methanomethylovorans hollandica DSM 15978. 1214 Methanomicrobium Medium, 1115 Melhanomicrobium mobile. 1115. 1116 Methanomicrobium mobile Medium. 1115 Melhanomicrobium paynteri, 1117 Methanomicrobium paynteri Medium, 1116

Index

Methanomicrococcus blatticola. 1118 Methanomicrococcus Medium, 1117 Methanomonas Autotrophic Medium, 1117, 1118 Methanomonas methylovora. 1111. 1140, 1924 Meihanomonas species, 1118 Methanoptanus limicola, 1104 Meihanoplanus species. 1104 Melhanopyrus ka tidieri, 1119 Meihanopyrus Medium. 1118 Methanosaeta conciiii. 1138 Methanosarcina acetivorans. 1119, 1120 Methanosarcina acetiwrans Medium, 1119 Meihanosarcina barkeri, 1084. 1085. 1120. 1125 Meihanosarcina barkeri Medium, 1120 Meihanosarcina BCYT Medium, 1120 Meihanosarcina DPB Medium, 1121 Methanosarcina fnsia, 710, 1084, 1085 Meihanosarcina frisia Medium, 1122 Meihanosarcina Mass Culturing Medium. 1122 Methanosarcina mazei. 710, 756. 1084, 1085,1123,1125 Meihanosarcina mazei Alpha Basal Medium. 1123 Meihanosarcina mazei Medium. 1123 Methanosarcina Medium, 1123. 1124, 1125 Methanosarcina MP Medium. 1126 Methanosarcina Nitrogen-Fixing Medium, 1127 Methanosarcina semesiae. 1128 Meihanosarcina semesiae Medium, 1127 Meihanosarcina species. 710. 1114,1121. 1122,1123, 1125, 1126,1127, 1247 Meihanosarcina thermophila. 710, 1125, 1129, 1130,1731 Meihanosarcina Thermophilic Medium, 1128.1129 Meihanosarcina vacuolala, 1125 Mefhanosphaera ciiniculi. 1132 Melhanosphaera Medium. 1, 1130 Mefhanosphaera Medium. II, 1131 Melhanosphaera stadlmanae, 1131 Methanosphaerula Medium, 1132 Methanosphaerula species, 1133 Mefhanospirillmn hungatei. 1134, 1135. 1136 Methanospirillum hungatei JMA Medium. 1133 Methanospirillum hungatei Medium, 1134 Methanospirillum hungatei SAM Medium, 1134 Methanospirillum SK. Medium, 1135 Melhanothennohacterlhennautolrophicus, 1080 Meihanolhennobacler/Methanobacierium Transduction Agar. 1136 Melhanolhermohacler/Methanobaclerium Transduction Medium. 1136 Mefhanolhennusfenidtts. 1137

Methanothermtis fervidus Medium. 1137 Methanoihermus sociabilis, 1138 Methanothri.x Medium, 1138 Methanolhrix thermophila, 1732 Methermicoccus Medium, 1139 Melhermicoccus species, 1139 Methicillin-rcsistant Staphylococcus aureus. 841,1069 Methyl Red test. 1236.1237 MeUiyl Red-Voges-Proskauer Broth, 1236 Methyl Rcd-Voges-Proskaucr Broth with NaCl, 1237 Methyl Red-Voges-Proskaucr Medium. 1237 Methyl Rcd-Voges-Proskauer Broth with NaCl, 1237 Methyl Rcd-Voges-Proskauer Medium, 1237 MeUiylamme Salts Medium, 1139 Methylene Blue Milk Medium, 1139 Methylobacillusfructoseoxidans, 1154 Meihylobacillus glycogenes, 914. 1112 Methylolutcillus glycogens. 1110 Melhylohacillus pratensis, 1053 Methylolxicterium Agar, 1140 Methylobacierium extorquetts, 435. 444, 1112,1139 Methylobacierium fujisawaense, 1112, 1292 Methylobacierium lusitanwn, 1048 Methylobacierium Medium, 1140 Methylolxicterium mesophilicum, 116. 1112. 1403 Methylobacierium organophilum. 1112. 1140 Methylobacierium podarium, 105 5 Melhylolxtcierium nuliotolerans. 1112 Methylobacierium rhoiiesianwn, 1111, 1112 Methylobacierium HUM!mum. 1112, 1291

Methylobacierium species, 95, 435, 444, 1110,1111,1112. 1141,1291.1292. 1924 Methylobacierium suomiense. 1048 Methylobacierium thiocyanalum, 1141 Methylobacierium thiocyanalum Medium, 1141 Methylobacierium zatmanii. 1112 Methylocapsa acidiphila. 1141 Methylocapsa acidophila Medium, 1141 Methyloceila silverstris. 1142 Methyloceila silverstris Medium., 1141 Methyloceila lundrae. 1171 Methylococcus capsulatus, 912, 1291 Methylococcus Medium. 1142 Methylococcus species, 1142 Methylocystis heyeri. 1171 Methylocyslis hirsula, 1299 Methylohalobius crimeensis. 803 Methylohalomonas Medium, 1142 Methylohalomonas Medium with Acetate. 1143 Methylohalomonas species. 1142. 1143 Methylomicmbium Medium, 1143

2001

Methylomicrobiuin species, 1144 Methyiomonas agile, 1291 Methylomonas clara. 1292,1299. 1310 Methyiomonas methanica, 1291 Methylomonas methylotrophus, 1110 Methyiomonas species, 95, 1112 Melhylonatnun Medium,, 1144 Methylonalnnn Medium with Acetate, 1144 Meihylonaintm species, 1144, 1145 Melhylophaga Agar, 1145 Methylophaga alcalica, 1146 Melhylophaga alcalica Agar. 1145 Methylopiutga alcalica Medium. 1146 Methylophaga Broth, 1146 Methylopiutga marina. 1017, 1018. 1055. 1113,1145,1147 Methylophaga Medium, 1147 Melhylophaga suljidowrans. 1147 Melhylophaga sitlftdovorans Medium, 1147 Methylopiutga thalassica, 1017. 1018.1055, 1113,1147 Methylopiutga thalassica Agar. 1147 Melhylophilus glucttseoxiduns. 1154 Methylophilus leisingeri. 1176 Melhylophilus methylolrophus, 435,444, 1110,1112,1197 Melhylophilus species, 95. 1017, 1018 Melhylosarcina fibrata, 1148 Methylosarcina qttisquiliarum. 1148 Methylosinus trichaiporium, 95 Mcthylotrophic Arihrolxtcter Ilyphomicrobiwn Medium, 1148, 1149 Methylovorus glucosotrophus. 1112 Methylovorus mays. 1053 Methylpyridine Medium, 1149 Melschnikowia hawaiiensis, 1001 Melschnikowia pulcherrima. 1000. 1942 Melschnikowia reukaufu, 1000 MP I-ndo HiVeg Medium, 1067 M-FCAgar, 1150 M-FC Agar, 669 M-FCAgar Base. 1151 M-FC Broth. 669 M-FC HiVeg Agar Base with Rosalie Acid, 1150 M-FC HiVeg Agar Base, Modified with Rosalie Acid, 1150 M-FC HiVeg Broth Base with Rosalie Acid. 1150 M-Fecal Col i form Agar, 669 M-Fecal Colifonn Agar, Modified. 670 M-Fecal Colifonn Broth, 669 MG Medium. 1151.1152 MCA Agar, 1153 M-Cireen Yeast and Mold Broth. 761 MGTY Agar, 1016 MGTY Broth, 1016 Mil Agar, 15%, 1153 Mil 111 Agar, 1153 Mil Medium, 1154 Mil Medium. 15%. 1155 Mil Medium. 10%, 1154

2()ii2

Index

Mil Medium. 2%, 1154 MH Salts, 1155 M-HPC Agar, 857 Miamiensis avidia, 989

MIB with Maltose, 1155 MIC testing, 1906 Micro Assay Culture Agar. 1155 Micro Assay Culture Agar, 1155 Micro Assay Culture Broth, 1156 Micro Inoculum Broth, 1156 Micro Vitamin Test Culture I liVeg Agar. 1156 Micro Vitamin Test Inoculum HiVeg Broth, 1156 Microaerophiles, 17, 18, 1772, 1773 Microacrophilic bacteria. 101.256.694.695, 1774 Microascus irigonosporus, 706 Microbacterium arhorescens, 1429, 1470 Xficrobacterium Jlavescens, 1304 Microhacterium halotolerans, 1214 Microbactcriuin imperiale, 454, 1470 Microbacterium lacticum, 454, 1470 Microhacterium laevaniformans, 453, 454 Microbacterium Medium, 1156 Microbacterium species. 1156. 1830. 1890. 1941 Microbacterium ulmi, 1918 Microbial Content lest Agar. 1156 Microbial Content Test I liVeg Agar, 1156 Microbial plate counts. 1631 Microbiological assay, 222 Microbispora bisfwra, 1322 Microbispora chromogenes, 1939 Microbispora rosea. 480.638. 743. 1321. 1322,1323, 1330.1411, 1517 Microbispora species. 1637 Microbispora thermodiastatica. 210. 1939 Microcella alkaiiphila, 1157 Microcella alkaiiphila Medium. 1157 Micrococcus agilis, 454 Micrococcus aurantiacus. 1309 Micrococcus halohius. 454, 455, 791, 1343 Micrococcus kristinae. 454 Micrococcus lutein, 134,644, 1158,1282, 1283,1308. 1382. 1429.1456. 1464. 1647 Micrococcus fylae, 454 Micrococcus Medium, 1157 Micrococcus Medium, FDA, 1157 Micrococcus nishinomiyaensis. 454 Micrococcus roseus, 454 Micrococcus sedentarius. 454 Micrococcus species, 117,454, 673. 1157, 1311 Micrococcus various, 454, 792, 1343 Micrococcus/Sarcina Medium, 1158 Microcyclus aqualicus, 320 Microcyclus eburneus. 1158 Microcyclus eburneus Medium. 1158 Microcyclus major. 1158 Microcyclus marinus, 1158

Microcyclus martnus Medium, 1158 Microcyclus Medium, 1158 Microcystis species, 1158 Mtcrocyclus/'Spirosoma Medium, 1158 Microcylus major Medium. 1158 Microcystis aeruginosa, 758 Microdochium nivaie. 1418 Microlunatus Medium, 1158 Microlunatus phosphovorus, 1159 Micromonas micros, 1363 Micromonospora aurantiaca. 1159 Micromonospora brunnea. 481, 1159 Micromonospora chalcea, 481, II59, 1939 Micromonospora coendea, 210, 638, 1517 .Micromonospora echinospora. 210 Micromonospora glauca. 1159 Micromonospora haiophytica, 1159 Micromonospora inositola, 1159, 1319 Micromonospora megalomicea Agar, 1159 Micromonospora melanosporea, 1159 Micromonospora narashino, 743 Micromonospora purpurea. 210, 481, 1159 Micromonospora purpureochromogenes, 210,481,1159 Micromonospora rhodorangea, 1159 Micromonospora rosaria, 210. 1159 Micromonospora ruminantium. 616,617 Micromonospora species, 210,439,481,758, 980, 1318,1319, 1321,1323.1574 Micromonospora Starch Agar, 1159 Micropolysporafascifera. 1645 Microscilla aggregans, 808 Microscilla arenaria, 1054 Microscilla J'un<escens. 1054 Microscilla marina, 1054 Microscilla sericea, 1054 Microscilla species. 477, 1015 Microspora species, 159, 320, 444, 907. 1153.1318,1877.1927 Microsporum audovini, 1513 Microsporum canis, 1529, 1532 Microsporum distortion. 452 Microsporum persicolor, 265 Microsporum species. 1513 Microstreplospora cinerea, 1323, 1330 Microtefraspora africana. 1323,1929 Microtetraspora angiospora, 1939 Microtetrasporafastidiosa, 1322. 1323 Microletnispora ferruginea. 1323. 1939 Microtetraspora flexuosa, 210, 1323 Microtetraspora fusca, 758 Microtetraspora giauca, 481, 1322 Microtetraspora helvala, 210 Microtetraspora incanescens, 1939 Microtetraspora niveoalba, 1939 Microtetraspora polychroma, 1322 Microtetraspora pusilla. 1322 Microtetraspora recticatena, 639 Microtetraspora roseola. 1323 Microtetraspora rosewlacea. 1323 Microtcfnispora rubra, 1323 Microtetrasjyora salmonea, 1323

Microtetraspora spirtdis, 1939 Microthecium retisporum, 944 Microthelia albidella. 951 Microvirgula Medium. 1159 Middlebrook 13A Medium, 1159 Middlcbrook 71110 Agar with Middlebrook ADC Enrichment. 1161 Middlcbrook 7H10 Agar with Middlebrook OADC Knrichmcnt. 1161 Middlebrook 71110 Agar with Middlebrook OADC Enrichment and Ilemm. 1162 Middlebrook 71110 Agar with Middlebrook OADC Enrichment and Triton™ WR. 1339,1162 Middlcbrook 71110 Agar with Streptomycin. 1163 Middlcbrook 71111 Agar with Middlcbrook ADC Enrichment, 1163 Middlcbrook 71111 Agar with Middlcbrook OADC Enrichment, 1164 Middlcbrook 71111 Agar with Middlebrook OADC Enrichment and Triton™ WR. 1339, 1164 Middlcbrook 71111 Agar. Selective. 1163 Middlebrook 71111 1 liVeg Agar Base with

Middlebrook ADC Enrichment, 1165 Middlebrook 71111 IhVeg Agar Base with Middlcbrook OADC Enrichment, 1165 Middlcbrook 71112 Medium. 1165 Middlebn>ok 7119 Broth with Middlebrook ADC Knrichmcnt. 1160 Middlcbrook 7119 Broth w itli Middlebrook OADC Enrichment. 1160 Middlebrook 7119 Broth with Middlebrook OADC Enrichment and Triton™ WR 1339,1160 Middlcbrook 7119 Broth, Supplemented, 1160.1161 Middlebrwk AIX' Enrichment. 5,1159 Middlebrook Albumin l>extrose Catalase lvnriehmetit, 1159 Middlcbrook and Cohn. 71110 Agar. 1161 Middlcbrook Medium, 1166 Middlcbrook Medium widi Mycobactin. 1166 Middlebrook OADC Enrichment. 5.1166 Middlcbrook OADC Enrichment with Triton™ WR 1339.1167 Middlebrook Oleic Albumin Dextrose Catalase Enrichment, 1166 Middlebrook Oleic Albumin Dextrose Catalase Enrichment with Triton™ WR 1339, 1167 Mil. I liVeg Medium, 1223 MIL Medium. 1167 Mildew, 16 Milk, 130,133.206,274,353,645.646.701. 843,932,954, 955,958, 992,993, 1403, 1404.1405.1631, 1823.1838. 1839. 1840, 1953 Milk Agar. 1167. 1575 Milk carbohydrate fermentation. 880

Index

Milk MiVeg Agar. 1167, 1168 Milk Protein Hydrolysate Agar, 1228 Milk Salt HiVcg Agar Base. 1168 Miller I.uria Bertani IliVeg Agar, 1168 Miller Luria Bertani IliVeg Broth. 1168 Mima potymorpha, 1561 Mineral Agar, 1168 Mineral Base E lor Autotroj)hie Growth, 1168 Mineral Base I- for I leterotrophic Growth, 1169 Mineral Base Medium with Acetate. 1031 Mineral Lactate Medium, 1169 Mineral Medium, 1170 Mineral Medium (N4). 1178 Mineral Medium A. 1171 Mineral Medium for Chcmolithotrophic Growth, 1173 Mineral Medium lor I lydrogen Bacteria, 1177 Mineral Medium 11-3. 1176 Mineral Medium M9, 1177 Mineral Medium S with 1% Sucrose, 1182 Mineral Medium with 2.4-Dichlorophcnoxyacctic Acid, 1176 Mineral Medium with 2.4-Dichlorotolucne. 1176 Mineral Medium with 2-Chlorobenzoate, 1174 Mineral Medium with 2-IIydroxybiphenyl, 1177. 1178.1180 Mineral Medium with 3-Aminophenol, 1171 Mineral medium with 6-Mcthylnicotinatc. 1178 Mineral Medium with Antipyrin, 1171 Mineral Medium with Atrazine. 1172 Mineral Medium with Ben/ylcyanide, 1172 Mineral Medium with Camphor. 1173, 1176 Mineral Medium with Chlorida/on, 1173 Mineral Medium with Crude Oil. 1174 Mineral Medium with Cyanuric Acid as Nitrogen Source, 1174 Mineral Medium with Dichlorobenzoate, 1174.1175 Mineral Medium with Dichloromethane. 1175 Mineral Medium with Glucose, 1176 Mineral Medium with Na|)htlialene, 1179 Mineral Medium with Nicotinic Acid. 1179 Mineral Medium with o-Nilrophenol, 1179 Mineral Medium with PCP, 1180 Mineral Medium with Phenol, 1180 Mineral Medium with Phcnylacetatc. 1181 Mineral Medium with Pyrrolic Acid. 1181 Mineral Medium with Quinoline. 1181 Mineral Medium with Salicylate, 1182 Mineral Medium with Santonin. 1182 Mineral Medium with Sull'obenzoic Acid, 1183 Mineral Medium with Toluene. 1183 Mineral Medium with Vitamins. 1183 Mineral Medium, 1170,1171

Mineral Medium. Brunner, 1173 Mineral Medium. Nagel and Andreesen, 1178 Mineral Medium, pi I 7.25, 1180 Mineral Pectin 5 Medium. 1227 Mineral Pectin 7 Medium, 1228 Mineral Salts Agar. 1184 Mineral Salts Enrichment Medium, 1184 Mineral Salts for I ricrmophilcs, 1186 Mineral Salts Medium, 1184 Mineral Salts Medium for Thcrmophilcs. 1185 Mineral Salts Medium with Methanol. 1185 Mineral Salts Medium with Methanol and Yeast Extract, 1185 Mineral Salts Peptonized Milk Agar. 1185 Mineral Salts with Butane. 1184 Minerals Modified Glutamatc Agar. 1186 Minerals Modilicd Medium, 1186 Minimal Agar I, 1186 Minimal Agar II, 1187 Minimal Agar III, 1187 Minimal Agar, Davis, 1187 Minimal Broth 1.1188 Minimal Broth II, 1188 Minimal Broth III. 1188 Minimal Broth, Davis, 1189 Minimal F-Top Agar. 1189 Minimal Lactate Medium, 1196 Minimal Medium for Denitrifying Bacteria. 1189 Minimal Medium for Penicillium Interspecific Hybrids. 1189 Minimum I Essential Medium with Bicarbonate, Scrum, and Antibiotics. 1189 Minimum Salts wiUi IliVeg Acid Hydrolysate. 1190 MIO Hi Veg Medium. 1190 MIO Medium. 1223 Mischococcus sphaerocephalus. 70 Mist Agar, 1191 Mitis Salivarius Agar, 1191 Mitis Salivarius Hi Veg Agar Base with Tellurite, 1191 Mitrophora semilihera, 1002

Mitsuokella dentalis. 1192 Mitsuokella dentalis Medium, 1191 Mitsuokella jalaludinii, 1487 Mitsuokella multiacuia. 631. 718 Mitsuokella multiacidus. 1459 Mixed Cereal Agar, 1192 Mixotrophic, 1759 Mixotrophic Nitrohacter Medium, 1192 Mixotrophic Nitrobacter Medium, 10%. 1192 Mixotrophic Nitrobacter species, 1192 MJ Medium for Ihiobacter subterraneus. 1194 MJ Medium, 1193 MJANI IOX-N03 Medium with Supplement. 1195 M-Kleb Agar, 902

2(K)3

Ml. Medium, 11% M-Lautyl Sulfate Broth, 932 M-Lauryl Sulfate HiVcg Broth. 1196 MI .CI J Agar. 1196 M-I.KS, 1-ndo Agar, 641 MLGA, 1066 MMAgarHiCromeIM.841 MM 10 Agar, 11% MM A Salts Medium. 1197 MMB Medium. 1197 MMJS Medium (Modilied), 1198 MMNAgar, 1198 MMS Medium for Thermotoga neapoliiana. 1199 MN. 1064 Mn Agar No. 1, 1004 Mn Agar No. 2, 1005 MN Marine Medium, 1199 MN Marine Medium with Vitamin B 12 . 1200 Mobilunetis Agar. 1200 Mobilunetis cunisii, 441, 659 Mobilunetis mulieris. 441, 659 Moderate Halophilic Medium (HM). 1200 Modified AHA Sporulation Medium Base, 1200 Modified Biebl and Pfennig's Medium.. 1201 Moditied Bile I-sculin A/ide Agar, 1201 Modified Buffered Charcoal HiVcg Agar Base with Cysteine, 1201 Modified Campylobacter Blood-Free Selective Agar Base, 1202 Modified Campylobacter Charcoal Differential Agar, 1202 Modified CCDA, 1202 Modified CPI.M IliVeg Medium Base with Horse Serum, 1202 Modified CPI.M IliVeg Medium Base with Horse Serum. Penicillin, Streptomycin, and Nystatin. 1202 Modified Differential Clostridial Broth, 1203 Modified Duncan Strong I liVcg Medium. 1203 Modified Duncan Strong Medium, 1203 Modified Fungal Agar Base. 1203 Modified Fungal IliVeg Agar Base, 1203 Modified FWM Medium. 1204. 1205,1206. 1207,1208,1209,1210,1211, 1212, 1213 Modified Gorodkowa Agar. 1214 Modified Inhibitory Mold Agar. 1203 Modified Inhibitory Mold IliVeg Agar Base. 1203 Modified ISP5 Medium with Sodium Chloride, 1214 Modified ISP5 Medium.. 1214 Modified I.etheen IliVeg Agar, 1215 Modified Lcthccn HiVcg Broth, 1215 Modified MacConkey Medium, 1037 Modified Marine Broth. 1215 Modified McBnde Listeria I li Veg Agar Base with Blood and Cycloheximide. 1215 Modified Medium. 10 Agar. 1196

2004

Index

Modified MYP Agar Base, 1216 Modified MYP HiVeg Agar Base wilh Fgg Yolk and Polymyxin B. 1216 Modified Ox lord Antimicrobic Supplement, 7 Modified Oxford Listeria Selective Agar, 1216 Modified PYNFH Medium. 1217 Modified Raggios Medium, 876 Modified RajipajxHt Vassiliadis HiVeg Medium, 1217 Modified Rogosa HiVeg Agar. 1217 Modified Semisolid Rappaport Vassiliadis Medium, 1217 Modified Shieh Agar, 1217 Modified Skim Milk HiVeg Agar. 1218 Modified Soyabean Bile BroUi Base, 1218 Modified Soybean HiVeg Agar. 1218 Modified Tli Agar, 1218 Modified Thayer-Martin Agar, 1707. 1708. 1709 Modified Thermits Medium, 1218 Modified V.P. HiVeg Broth, 1219 Modified VWM Medium. 1219 Moeller Decarboxylase Broth, 1220 Moeller Decarboxylase HiVeg Broth Base. 1220 Moeller Decarboxylase HiVeg Broth with ArginineHCl. 1220 Moeller Decarboxylase HiVeg Broth with Lysine HC1, 1220 Moeller IX*carboxylase HiVeg Broth with Ornithine HCI. 1221 Moeller KCN Broth Base. 1221 MOF HiVeg Medium. 1018 MOF I liVeg Medium widi Carbohydrate, 1221 Molds, 54. 55, 123, 128, 133, 232,233, 245. 249, 250, 254, 284,318, 353,469,482, 593.607.638.692.810.843.998. 1000. 1001, 1002, 1003. 1203, 1261, 1337, 1411.1412.1413,1414.1415. 1416, 1417,1517, 1530. 1533, 1910, 1911. 1912,1934 Molybdatc Agar. 1221 Xionascus bisporns, 988 MonilielJa pollinis. 999. 1258 Xioniliella sttaveolens, 1004 Xioniliniafrueticoia. 1410 Monkey kidney cells, 1301 Xionoblepharis polymorpha, 1405 Xfonocerxomonas colubrorum. 467 Xionocercomonas species. 1861 Xionochaetia malt, 952 Xionodictys austrina. 1534 Mono Jus sttbterranens, 70 Xionasiga brevicollis, 1556 Monsur Agar, 1222 Xioorella glycerin!, 1222 Moorella glycerini Medium, 1222 Xioorella mtdderi, 1616 Xioorella thermoacelica. 420,423. 1616

Moraxella allantae, 1223, 1304 Xioraxella bovis, 306, 454, 455 Moraxella calorrhalis. 215 Xioraxella laatnata, 963, 958 Xioraxella lincolnii, 298. 1249 Xioraxella Medium, 1223 Moraxella nonliqttefaciens, 306, 721, 813 Xioraxella osloensis, 306, 1223, 1248 Xioraxella species. 1173, 1827 Xioraxella urethralis, 215 Xiorchella escttlenla. 1002 Xioritella japonica, 1010 Morococcus cerebrosis, 721 Xiortierella btsporalis, 809 Xiortierelia httmilis, 452 Xiortierella hygrophila, 452 Xiortierella minutisshna, 452 Xiortierella species, 930 Most-probable- number. 11 Motile Salmonella species, 1217 Motility. 200. 466,621,1167. 1191. 1223. 1224,1225.1226.1227. 1563,1572, 1823 Motility Gl Medium. 1223 Motility Indole Lysine Medium, 1167 Motility Indole Ornithine HiVeg Medium. 1190 Motility Indole Ornithine Medium. 1223 Motility Medium, 1223 Motility Medium S. 1224 Motility Nitrate Agar, 1224 Motility Nitrate HiVeg Medium, Buffered, 1224 Motility Nitrate Medium, 1224 Motility Nitrate Medium. Buffered. 1225 Motility Sulfide Medium, 1225 Motility 'lest and Maintenance Medium. 1225, 1226 Motility Test and Maintenance Medium, Gilardi, 1226 Motility Test and Maintenance Medium, Tatum. 1226 Motility Test HiVeg Medium. 1225 Motility Test HiVeg Medium with Triphcnyltetrazohum Chloride. 1225 Motility Test Medium, 1226 Motility Test Medium, Semisolid, 1226 Motility lest Medium. Semisolid with Sodium Chloride, 1227 Motility-Indole-Lysine HiVeg Medium, 1223 Mouse adrenal assay, 807 MOX Agar. 994. 1216.1334 MOX HiVeg Agar, 1227 MP 5 Medium. 1227 MP 7 Medium, 1228 MP Agar. 1227 M-PA Agar, 1439 MPA-C Agar. 1338 MPHAgar, 1228 MPHM Medium, 1228 M-Plate Count Broth, 1403

MPOB Medium, 1229 M-Pseudomonas aeruginosa Agar, 1439 MPSS Broth, 1230 MPYAgar, 1230 MPY Broth. 1230 MPYG Medium, 1368 MRS Agar. 1231 MRS Agar with 0.5% Cysteine, 1231 MRS Agar with Cysteine. 1231. 1236 MRS Agar with Hthanol, 1232 MRS Agar with Tomato Juice. pH 5.2.1132 MRS Agar. Half Strength, 1232 MRS Broth, 1232. 1233 MRS Broth with CaC0 3 , 1233 MRS Broth with Cysteine. 1233 MRS BroUt with l-thanol, 1233 MRS Chalk. 1234 MRS fructose Medium, 1234 MRS I liVeg Agar, 925, 1234 MRS HiVeg Agar, Modified. 1234 MRS HiVeg Broth. 925 MRS HiVeg Broth, Modified. 1235 MRS Medium. 1235 MRS Medium pH 5.5,1236 MRS Medium with I.-Cysteine, 1236 MRS Medium, Modified. 1235 MRS Salts, 1236 MRS with Fermented Wort, 1234 MRS, Modified, 922, 923 MRSA. 259. 377.841.1069. 1236 MRSASelectâ&#x201E;˘, 1236 MRVP Broth. 1236 MRVP Broth with Sodium Chloride. 1237 MRVP Medium, 1237 MS Agar, 1237 MS I Agar. 1237 MS 3 Agar. 1237 MS 4 Agar, 1238 MS Medium, 1238.1239 MS Medium (Modified), 1240 MS Medium for Acidiphiilum cryptum, 1239 MS Medium for I.epiospirillum species, 1239 MS Medium for Methanogens, 1239 MS Medium for mbacillus caldus, 1241 MS Medium for 'iliibacilltts ferrooxidans, 1241 MS Medium for niobacillus tbiooxidani, 1241 MS.0157 Agar IliCromeâ&#x201E;˘, 841 MS.0157 Agar with Tellurite, HiCromc1M, 842 MSA-Fc Medium. 1241 M-Seven-Hour Fecal Coliform Agar, 1567 MSGM. Revised, 995 MSI .86 Medium,, 1242 M-Slanet/. Enierococcus Broth Base with Triphenylteurazolium Chloride, 1241 M-Slanet/. Enterococcus HiVeg Broth liase with 1 riphenyltetrazolium Chloride, 1242 M-Sporulation Agar. 1617 MSRV, 1482

Index

MSRV Medium. 1217 MSRV Selective Supplement, 7 M-ST Holding Medium. 1626 M-Standaixl Methods Broth, 1631 ^Staphylococcus Broth, 1633 MStaphylococcus HiVeg Broth, 1242 MSV AcS Agar, 1243 MSVAgar, 1243 MSV Broth, 1244 MSVGSAgar, 1244 MSV I Agar. 1244 MSV IT Agar, 1245 MSV S Agar. 1245 MSV SS Agar, 1245 MSV SUC Agar. 1246 MS VP Agar, 1246 M-T7 Agar Base, 1682 M-TLCAgar, 1690 M-Tccpol Broth. Hnriched, 1690 M-Tetrathionate Broth, 1700 M-Telrathionate I liVeg Broth Base with Iodine, 1247 MTMI1, 1707 MTP4 Medium, 1247 MTSB), 1832 M-TT Broth. 1700 Mutate Broth, 1248 Mucate Control Broth, 1248 Mucate Control HiVeg Bixuh, 1248 Mucate HiVcg Broth. 1248 Mucilaginibacler gracilis, 1805 Mucor hiemah's, 251 Mucor ramannianus NRRI., 1839, 1646 Mucor species, 658. 1672 Mud, 419, 1096, 1607 Mueller-Hinlon Agar, 1248 Mueller-Hinton Broth, 1249 Mueller-! linlon Chocolate Agar, 1249 Mueller-Hinton Agar with Horse Blood, 1248 Mueller-I hnton Agar with IsoVitaleX* and Hemoglobin, 1249 Mueller-I hnton Agar with Sodium Chloride. 1249 Mueller-Hinton HiVeg Agar, 1250 Mueller-Hinton HiVeg Agar No. 2. 1250 Mueller-Hinton HiVeg Broth. 1250 Mueller-Hinton II Agar. 1250 Mueller-Hinton Medium with Garden Soil, 1250 Mueller-I hnton Medium with Rabbit Serum, 1250 Mueller-Kauffman Tetrathionaie HiVeg Brotli Base with Iodine and Brilliant Green, 1251 Mueller-Kauffmann Telrathionate Broth, 1250 Mueller-Tellurite Medium, 1251 MUG Bromcrcsol Purple Broth with lactose. 1251 MUG EC Broth. 1252 Ml G LC Broth. Modified, 1252

MUG 1-C HiVeg Broth, 1252 MUG I-COI57 Agar, 1252 MUG I-C ()157 Agar. Modified, 1252 MUG I.auryl Sulfate Broth, 1252 MUG Lauryl Sulfate Broth, Modified. 1253 MUG I .aurvl Sul fate I liVeg Broth, Modi lied, 1253 MUG Nutrient Agar, 1253 MUG Plate Count Agar. 1253 MUG Sorbitol Agar, 1253 MUG Tryptonc Soy Agar, 1254 MUG Violet Red Agar, 1254 MUG Violet Ret! HiVeg Agar, 1254 Multiple lube technique. 153, 1381 Multiple-tube fermentation tccluiique. 932 Murtcaiida ruestringensis. 1555 Marietta aurantiaca, 70 Marietta decolor. 70 MV Medium, 1254 MVL Medium. 1255 MV'IT Medium, 1255 MWY Medium. 1256 MY, 40G Agar, 1257 MY Agar, 1256 MY Medium, 1257 MY 10-12 Medium. 1257 MY 10-12 Medium,, 1257 MY20 Agar, 1256 MY40Agar, 1257 MY40G. 1003 MY50G, 1003 MY60 Agar, 1257 MYA2. 1004 MYA4, 1004 MYA4 with 40% Sucrose, 1257 MYA4 with Lupine Stems, 1004 MYA8, 1004 MYCA Medium, 1258 Mycelial phase, 850 Mycin Assay Agar, 1258 Mycoarachis inversa. 1476 Mycobacteremia patients, 1159, 1166 Mycobacteria, 1327. 1540 Mycobacteria 71111 Agar with Middlelwook ADC I-Jirichmcnt. 1163 Mycobacteria TIIII Agarwitli Middlebrook OAIXM-nrichment. 1164 Mycobacteria 71111 Agar witli Middlebrook OA1X.* Enrichment and Triton™ WR. 1339.1164 Mycobacterium acapulcensis, 1470 Mycobacterium Agar. 1258 Mycobacterium avium,967, 968, 1161,1259, 1364 Mycobacterium avium subsp. paraluberado5«, 1166 Mycobacterium ftovis, 968,1640.1686,1688 Mycobacterium chelonae, 150 Mycobacterium chitae. 746 Mycobacterium diernhoferi, 214 Mycobacterium fotluitum, 150. 1330. 1862 Mycobacterium gastri, 1364

Mycobacterium Mycobacterium Mycobacterium Mycobacterium Mycobacterium 1271 Mycobacterium 1330.1364 Mycobacterium Mycobacterium Mycobacterium Mycobacterium Mycobacterium Mycobacterium Mycobacterium Mycobacterium Mycobacterium

2(K)5

glfoum, 1404 gordonae. 967,968 haemophilum, 1162 inlracelhdare, 1364 inlracelhdare Agar. 1258. kansasit, 967, 968, 1163, leprae, 157, 1900 marinum, 1364 Medium, 1258 microt, 1638 paraffinicum, 1258 paratuberculosis, 1259 phlei, 1258 scrofulaceum, 1364 smegmatis. 124.129, 753.

758,967,968, 1258,1373 Mycobacterium smegmatis Medium, 1258 Mycobacterium species. 150, 152. 157. 214. 605, 610, 611, 638, 751, 754, 767, 901, 1159,1160.1161.1162.1163.1166. 1167, 1179.1258.1259,1260. 1326, 1373, 1469,1470, 1567, 1686, 1688, 1856. 1900 Mycobacterium teirae, 1364, 1587 Mycobacterium tuberculosis, 46, 150, 157, 436.437,610,611,753,888.901.967, 968,1160.1161,1162.1163,1164,1165. 1435,1540,1683,1684,1685, 1686, 1688 Mycobacterium vaccae, 84, 453, 1330, 1364 Mycobacterium vanhaalenii, 215 Mycobacterium Yeast Lxlract Medium, 1259 Mycobactin Medium. 1259 MycobactoscIIM Agar. 1259 Mycobactoscl™ I.-J Medium. 1260 Mycobio Agar, 713 Mycobiotic Agar. 1260 Mycological Agar. 1260 Mycological agar, 711 Mycological Agar with Low pH, 711.1260 Mycological Broth, 712, 1261 Mycological Brotli witli Low pll. 1261 Mycological peptone, 2 Mycophil™ Agar, 1261 Mycophil™ Agar with I,ow pll, 1261 Mycophil™ Brotli, 1261 Mycoplana ramosa, 1456 Mycoplana segnis. 1456 Mycoplasma Agar. 1261.1262. 1263 Mycoplasma Agar Base, 1263 Mycoplasma Agar with Increased Selectivity. 1263 Mycoplasma Agar witli Supplement G, 1263 Mycoplasma Agar witli Supplement P, 1264 Mycoplasma alvi. 1595 Mycoplasma auseris. 1263,1265 Mycoplasma arginini, 1421, 1423 Mycoplasma Broth, 1264, 1265 Mycoplasma Broth Base. 1266

2006

Index

Mycoplasma Broth Base, l-'rey with 1 lorse

Scram, 1266 Mycoplasma Broth with 10% Swine Scrum. 1267 Mycoplasma Broth with Supplement G, 1266 Mycoplasma Broth with Supplement l\ 1267 Mycoplasma Broth, Supplemental. 1266 Mycoplasma collis, 1424 Mycoplasma columbinum. 1267 Mycoplasma columhorale, 1267 Mycoplasma dispar, 1270 Mycoplasma linrichment without Penicillin. 5 Mycoplasma equigenitalium. 812. 815, 816 Mycoplasma equirhinis, 253 Mycoplasma faucium, 1422 Mycoplasma fennentans, 1596 Mycoplasma flocculate, 1270 Mycoplasma gallinanun, 1620, 1621 Mycoplasma genitalium. 1597. 1620. 1621 Mycoplasma IliVeg Agar Base with Horse Serum and Yeast Kxtract. 1267 Mycoplasma 1 liVeg Broth Base widi Crystal Violet and Tellurite, 1267 Mycoplasma IliVeg Broth Base without Crystal Violet and with Ascitic Raid, 1268 Mycoplasma homims, 770, 771, 1874 Mycoplasma Horse Serum Broth. 1268 Mycoplasma hyopneumoniae, 1270, 1595 Mycoplasma hyorhinis. 707 Mycoplasma hyosynoviae, 1269 Mycoplasma iowae. 1472 Mycoplasma lipofuciens, 1263, 1265 Mycoplasma lipophilum, 1262. 1269 Mycoplasma Liquid Medium, 1268 Mycoplasma Medium. 1269 Mycoplasma Medium.. 1269 Mycoplasma Medium, Revised, 1269 Mycoplasma mustelae. 809 Mycoplasma mycoides, 1261.1265 Mycoplasma orale, 1383 Mycoplasma pneumoniae, 770, 771, 997, 998, 1270. 1287, 1595 Mycoplasma pneumoniae Isolation Medium, 1270 Mycoplasma putrefaciens, 1424 Mycoplasma species. 160. 288.809.816. 1262, 1263, 1264, 1266, 1267. 1268, 1269.1305.1420.1421.1422. 1423. 1424.1425. 1592 Mycoplasma suhdolum, 812,815,816 Mycoplasma Supplement. 5 Mycoplasma Supplement G, 7 Mycoplasma Supplement P. 7 Mycoplasma Supplement S, 7 Mycoplasma synoviae. 345 Mycoplasmal Agar, 1270 Mycoplasmas. 466 Mycoplasmatales, 1287 Mycorrhi/a Medium. 1270 MycoscIâ&#x201E;˘ Agar, 1270

Mycosphaerella ligtdicola. 972, 999 MYCT Medium. 1271 Mykorrhiza Agar, 1271 Myosateâ&#x201E;˘ peptone, 2 MYP, 1008 MYP Agar, 1008 MYP Agar Base, IliVeg with Hgg Yolk and

Polymyxin B, 1271 Mymides species, 1830 Mysorens Medium, 1271 MYX Agar, 1271 Myxobactcria. 88. 319, 341. 390.466.467, 468, 602, 624, 1037, 1447, 1476, 1569, 1593.1628.1629. 1634. 1898.1901 Myxobacteria Medium. 1272 Myxobacterium species, 189, 1186, 1370 Myxococcus amylovorans. 1898 Myxococcus coralloides, 1272 Myxococcus flavescens, 1272 MyxococcusJlavescens Medium, 1272 Myxococcusfulvus. 257,258.260, 288. 648, 890,1327,1530 Myxococcus Medium, 1272 Myxococcus species, 323, 1272, 1594 Myxococcus xanthus. 12.257.258.260.288. 648,979, 1038, 1272. 1327, 1526, 1530 Myxococcus xanihus Medium, 1272 Myxomicrobium multiplex. 1309 Myxosarcina species, 1200 Myxotrophic bacteria. 1227. 1256 N N DeVogel Medium, 1272 N plus C Medium, 1272 Ar-Acetylglucosamine Medium, 44 A-Acctylglucosamine utilization. 4-1 Nadi reaction. 60 Naegleria australiensis, 1217, 1549 Naegleriafowlen, 706,981, 1190, 1217, 1281, 1282, 1549 Naegleria gmberi, 706,981, 1217, 1454. 1552 Naegleria jadini, 1217 Naegleria lovaniensis, 706, 1301, 1303 Naegleria minor, 706 Naegleria thorntoni, 706 NAG Medium. 1273 Nakayama Glucose Agar, 1273 Naladixic acid resistant bacteria, 1311 Nalidixic Acid Novobiocin Actidione Tellurite Agar, 1274 NAM Medium, 1273 Names of media, 1 NAMn. 1309 NAN AT Agar, 1274 Nannocysiis Agar, 1274 Nannocystis species. 1274 Naphthalene Medium, 1274 Naphthalene Mineral Salts Medium. 1053 Naphthalene Sulfonic Acid Medium, 1274 Naphthalene utilization, 1179 Natranaerobius Medium, 1275

Nafranaerobius species, 1275 Nairialba species, 784 Natrinema ejinorense, 1053 Nairinema pallidum, 1346 Natroniella aceligena, 1276. 1277 Naironiella Medium, 1275, 1276 Natronincola histidinovorans. 1278 Nalronincola histidinovorans Medium, 1277 Natronobacteria Medium, 1278 Natronobacterium gregoryi, 83, 84, 777, 1278 Natronobacterium magadii, 83, 84, 777, 1278 Natronobacterium Medium, 1278 Natronobacterium pharaonis. 83, 84. 777, 788, 1278 Natronobacterium pharaonis Medium, 1278 Natronobacterium vacuolata. 84 Natronococcus occultus, 84, 777, 1278 Nautilia lithotrophica, 1279 Nautilia Medium. 1278. 1279 Nautilia profundicola. 89 NBA Medium. 1280 NBY Medium. 1280 Nectria haematococca, 1438 Nectria ipomoeae, 1438 Neisseria animalis, 588 Neisseria gonorrhoeae. 89.90. 324, 325. 360, 362, 441,445, 587,588, 725, 727, 728.730.731.758.821.997.1249.1710. 1807 Neisseria lactamica, 813 Neisseria Medium. 1281 Neisseria meningitidis, 324, 325,445, 1248, 1249.1281,1710 Neisseria meningitidis Medium. 1281 Neisseria species, 359. 360. 361. 445.466. 721,723,727,728,729,730,1248,1250, 1281. 1287.1437. 1438.1480. 1650, 1651. 1707,1708. 1709.1710, 1806. 1807 Neisseria weaveri, 1307 Nelson Medium tor Naegleriafowlen, 1282 Nelson's Culture Medium for Naegleria. 1281 Neocallimastix frontalis, 1282 Neocallimaslix Medium, 1282 Neomycin Agar. 1282 Neomycin Agar, Modified. 1282 Neomycin Assay Agar. 132 Neomycin Blood Agar, 1283 Neomycin l.uria Agar, 1283 Neomycin Medium No. 1, 1283 Neomycin. Krythromycin Assay Agar, 123 Neomycin. Krythromycin 1 liVeg Assay Agar, 128 Ncopeptonc, 2 Neopcptone Glucose Agar, 1284 Ncopeptonc Glucose Rose Bengal Aureomycin*'Agar, 1284 Neopcptone Infusion Agar, 1284 Neaspongicoccum excenlricum, 479

Index

Nephrochlamys subsolilaria, 70 NephrodieUa bre\>is, 70 Nesterenkonia halobia, 455 Neslerenktmia lacusekhoensLs, 635 Nesterenkonia luteo. 1215 Nesterenkonia Modified Medium.. 1284 Nesterenkonia species, 1284 Nesterenkonia xinjiangensis, 1214 Neufcld (Qiiellung) reaction. 1914 Neurospora crassa, 365. 1273.1909 Neurospora Culture Agar, 1284 Neurospora Medium, 1284, 1285 Neurospora Minimal Medium. 1285 Neurospora sitophila. 1284. 1286, 1464, 1909 Neurospora species, 1285. 1892.1893 Nevskia Medium, 1286 Nevskia ramosa, 1286 New York City Medium, 1286 New York City Medium, Modified. 1287 Niacin Assay HiVeg Medium, 1287 Niacin Assay Medium, 1288 Nickels and Leesment Agar, Modified. 1288 Nickcrson Medium, 217 Nicolinate utilizing bacteria, 1178 Nicotinic Acid Medium, 1288 Nicotinic acid utilization. 1179 Niger Seed Agar, 223 Niger Seed Salts Agar with Yeast Hxtract, 1289 Nigrosabuliim globosmn. 1476 Nigrospora sacchari, 944 Nigrospora sphaerica, 452 NIH Agar, 1289 NIH Thioglycolate Broth. 1289 Nlirrhioglyeollale Broth, 87 Nine K Medium, 1289 Nitrate Agar. 1289 Nitrate assimilation, 1904 Nitrate Assimilation Medium, Auxanographic Method lor Yeast Identification. 1289 Nitrate assimilation tests, 1290 Nitrate Broth, 1290 Nitrate Broth. Campylobacter, 1290 Nitrate Broth. Enriched, 1290 Nitrate I liVeg Agar, 1290 Nitrate HiVeg Broth, 1290 Nitrate Liquid Medium, 1291 Nitrate Metlumol Medium. 1291 Nitrate Mineral Salts Medium, 1291 Nitrate Mineral Salts Medium with Methanol. 1291 Nitrate reduction, 274, 874, 1224, 1289, 1290.1291.1292. 1646.1686.1821. 1840 Nitrate Reduction Broth. 1292 Nitrate Reduction Broth for Pseudomonas and Related Genera. 1292 Nitrate Reduction Broth, Clark, 1292 Nitrate-oxidizing bacteria, 1056 Nifralifacfor species. 1293

Nitratiruptor and Nitratifactor Medium, 1292 Nitratiruplor species. 1293 Nitrifying bacteria, 1909 Nitriliniptor alkaliphilus, 1294 Nitriliniptor alkaliphilus Medium,, 1293 Nilrilolriacelate Medium, 1294 Nilrincola Medium,, 1294 Nitrineola species, 1294 Nitrobacter agilis, 1294 Nitrobacter agilis Medium. 1294 Nitrobacter hamburgensis, 830. 1193 Nitrobacter Medium, 203, 1294 Nitrobacter Medium, 204. 1295 Nitrobacter Medium B. 1295 Nitrobacter species, 158, 1192. 1295 Nitrobacter vulgaris, 830, 1192, 1193 Nitrobacter winogradslni. 158,830. 1295 Nitrococcus Medium, 1296 Nitrococcus mobilis. 1295 Nitrococcus species, 12% Nitrogen Free Agar. 1297 Nitrogen source, 480, 482, 878, 998, 1770, 1872 Nitrogen utilization, 1927 Nitrogen-fixing bacteria. 285. 889 Nitrogen-fixing hydrocarbon-oxidizing bacteria. 12% Nitrogen-Fixing Hydrocarbon Oxidizers Medium, 1296 Nitrogen-Fixing Marine Medium. 1296 Nitrogen-Free Agar, 1296 Nitrogen-Free Medium for Pseudomonas stutzeri, 1297 Nitrogen-Free Mineral Agar for Derxia, 1297 Nitrogen-Free Mineral Medium for Beijerinckia, 1297 Nitrosocoecus Medium, 1297 Nitrosococcu.1 oceanus, 1297, 1298 Nitrosocoecus oceanus Medium, 1297 Nitrosolnhus Medium, 1298 Nitrosolobus multiformis, 1042. 1298 Nitrosomonas cryotolerans, 1557 Nitrosomonas europaea. 1012. 1298 Nitrosomonas europaea Medium, 1298 Nitrosomonas Medium. 1298 Nitrospira moscoviensis, 830, 1299 Nitrospira moscoviensis Medium, 1298 Nitsch's Trace Flements, 5 NL.333-AgarMalium.1299 NMS Medium, 1291, 1299 NMS Medium for Mcthanotrophs. 1299 NMS Medium with Methanol. 1291 NNN Medium. 1299 Noble agar, 2 Nocardia amarae, 743, 1330 Nocardia asteroides, 214.638,732.868,978. 1330,1416, 1929 Nocardia brasiliensis. 732.1330 Nocardia brevicalena, 214,481, 751, 1318 Nocardia calcarea, 214 Nocardia carnea, 1330, 1574

2007

Nocardia corynebacterotdes, 454. 1305 Nocardia crassostreae, 251 Nocardia diaphanozonaria. 743

Nocardia farcinica, 743, 1258, 1330, 1929 Nocardia globerula. 1330 Nocardia histidans, 1300

Nocardia Nocardia Nocardia Nocardia Nocardia Nocardia Nocardia Nocardia

histidans Medium. 1300 lucida, 1637 Medium. 1300 Medium, 1, 1300 Medium. 2, 1300 Medium, 3. 1300 Medium, 4. 1301 nitrifians, 1939

Nocardia otitidiscaviarum, 214, 743, 1330.

1574 Nocardia jwucivorans, 440 Nocardia petroleophila. 1309. 1330 Nocardia salmonicida, 743 Nocardia salmonicolor, 210 Nocardia seriolae, 1574 Nocardia species, 210. 286, 364. 454, 470, 639, 732, 751, 754, 767, 967,968, 980, 1173, 1300,1301.1309. 1326.1330. 1910, 1915,1934 Nocardia tarlaricans, 653 Nocardia transvalensis, 153, 1330 Nocardia vaccinii, 1330, 1364 Nocardia violaceofusca, 743 Nocardioides albus, 767, 1322, 1645, 1936 Nocardioides aquaticus, 635 Nocardioides faslidiosa, 743, 1470 Nocardioides fidvus, 1322 Nocardioides jensenii, 454 Nocardioides Iulcus. 1321 Nocardioides simplex, 454 Nocardioides species, 1321. 1330 Nocardiopsis albus. 767, 1645 NiKardiopsis arabia, 979 Nocardiopsis dassonvillei. 77, 638. 1645 Nocardiopsis kunsanensis, 431 Nocardiopsis mulabtlis, 1318 Nocardiopsis streptosporus, 1322 Nocardiopsis tropica, 215 Nocaridia asteroides, 1862 Nocaridia caviae, 1862 Nocariopsis species, 719 Nodulisporiiim griseobrunneum, 930 NaduHs/Kinum fuberum. 775 Nondairy foods, 645. 646 Nonfastidious, 107 Nonfat Dry Milk. Reconstituted, 1301 Nonfermentative Gram-negative bacilli. 1561. 1562 Nonfermentative Gram-negative bacteria. 20. 21 Nonfermenting Gram-negative bacteria, 22. 375, 1869 Non-lactose fermenting. 201 Nonnutnent Agar, 1301 Nonnutrient Agar Plates, 1301 Non-sporeforming anaerobes, 466

200S

Index

Nonsporulating anaerobes. 1906 Norris Agar, 1297 NOS Medium, Modified, 1301 NOS Spirochete Medium, 1302 Nosema corneum. 1190 Nostoc speeies. 85, 213, 214,133. 234 Novobiocin Agar. 1302 Novosphingobium acidiphilum, 890 Novy. MacNcal and Nicole Medium. 1299 NPB Medium.. 1303 NSMP. Modified. 1303 NTYG, 1303 Nuclearia species. 981 Nutrient, 1308 Nutrient Agar, 1303 Nutrient Agar, 1.5%, FliVeg, 1304 Nutrient Agar, 1.5%. HiVcg with Ascitic Fluid, 1305 Nutrient Agar No. 2.1305 Nutrient Agar No. 2 Diluted, 1/100, 1305 Nutrient Agar No. 2, Diluted, 1/10. 1305 Nutrient Agar No. 2, HiVcg, 1306 Nutrient Agar Oxoid CM3 with Phosphate. 1306 Nutrient Agar pH 10.0. 1308 Nutrient Agar pll 6.0, 1306 Nutrient Agar pll 6.0 with 0.8%NaCl, HiVcg. 1306 Nutrient Agar pi 16.8 with I lorse Blood, 1307 Nutrient Agar pH 6.8. HiVcg with Blood. 1307 Nutrient Agar pH 8.0. 1307 Nutrient Agar pll 9.0, 1307 Nutrient Agar pll 9.5,1307 Nutrient Agar with 0.5% Sodium Chloride, 1310 Nutrient Agar with 0.5% Sodium Chloride, pll 9.5-10.0,1310 Nutrient Agar with 0.5% Sodium Chloridcand Sodium Citrate, 1311 Nutrient Agar with 1.5% Sodium Chloride. 1310 Nutrient Agar with 1% Methanol, 1309 Nutrient Agar with 1% Peptone. HiVeg. 1306 Nutrient Agar with 10% Horse Scrum, 1309 Nutrient Agar witfi 10% Sodium Chloride, 1311 Nutrient Agar wim 10% Sodium Chloride and Maltose, 1311 Nutrient Agar with 2% Methanol. 1310 Nutrient Agar with 2% Sodium Chloride, 1310 Nutrient Agar with 2% Sucrose, 1312 Nutrient Agar with 3% Sodium Chloride. 1310,1311 Nutrient Agar with 5% Sodium Chloride, pi I 9.5-10.0.1311 Nutrient Agar with 5% Urea, 1313 Nutrient Agar with Cysteine. 1308 Nutrient Agar with Dihydrostreptomycin, 1308 Nutrient Agar with Erythromycin. 1308

Nutrient Agar with Ethanolamine. 1308 Nutrient Agar with Ethylene Glycol, 1308 Nutrient Agar with Formate. Fumarate, and Hoise Blood, 1304 Nutrient Agar with Glucose. 1308 Nutrient Agar with Horse Serum, 1309 Nutrient Agar with Maltose. 1305 Nutrient Agar with Manganese, 1309 Nutrient Agar with Manganese Sulfate. 1309 Nutrient Agar with Naladixic Acid, 1311 Nutrient Agar with Phyloncâ&#x201E;˘. 1312 Nutrient Agar with Potato Starch. 1312 Nutrient Agar with Soil Extract, 1312 Nutrient Agar with Streptomycin. 1312 Nutrient Agar with Sucrose, 1312 Nutrient Agar with Tetracycline, 1312 Nutrient Agar with Uracil, 1313 Nutrient Agar with Urcum, 1313 Nutrient Agar with V-8"â&#x201E;˘ Juice, 131.3 Nutrient Agar with Yeast Extract. 1313 Nutrient Agar, 1.5%, 1304 Nutrient Agar, Alkaline. 1304 Nutrient Agar, Buffered, 1304 Nutrient Agar. Half Strength, 1304 Nutrient Agar, pll 5.0, 1306 Nutrient Agar. pH 6.8. HiVcg. 1307 Nutrient Broth, 1313, 1314 Nutrient Broth Glycerol Medium, 1314 Nutrient Broth No. 2,1315 Nutrient Broth Sodium Chloride Thymine Medium. 1315 Nutrient Broth with 6% Sodium Chloride, 1314 Nutrient Broth with Bovine Serum, 1314 Nutrient Broth with Manganese Sulfate. 1314 Nutrient Broth with Rifampicin, 1314 Nutrient Broth with Streptomycin. 1315 Nutrient Broth Yeast Extract Medium. 1280, 1318 Nutrient Broth, 1/2 Strength. 1315 Nutrient Broth, Diluted El00,1314 Nutrient Broth. Standard II. 1315 Nutrient Brotlv-Salts Medium, 1315 Nutrient Gelatin, 1315 Nutrient Gelatin. CDC, 1316 Nutrient Glucose Agar, 1316 Nutrient HiVcg Agar, 1316 Nutrient I ii Vcg Agar for Oxidase, 1317 Nutrient Hi Veg Agar No. 2,1317 Nutrient 1 liVcg Agar pi 16.0 with 0.8% Sodium Chloride, 1316 Nutrient HiVcg Agar with 1% Peptone. 1317 Nutrient HiVeg Agar with Manganese, 1316 Nutrient Hi Veg Agar, 1.5%. 1316 Nutrient Hi Veg Broth, 1317 Nutrient Hi Veg Broth with 1% Peptone, 1317 Nutrient HiVcg Broth, pll 6.9 without Sodium Chloride, 1317 Nutrient Soil Extract Agar. 1317 Nutrient Yeast Glucose Medium, 1318 Nulrinet Agar pH 6.8,1306 Nutrinet Agar pi 16.8 with 1 lorse Blood, 1619

Nutritional mutants, 1187, 1189 NWRI Agar, 1318 NY Medium, 1318 Nystatin Assay Agar, 123, 133 Nystatin HiVcg Assay Agar. 128 N-/. Amine A Medium, 1318 N-Z Amine A Medium with Soluble Starch and Glucose. 1318 N-/. Amine Medium, 1318 N-Z Amine Glycerol Agar. 1318 NZC Broth, 1319 NZCYM Medium 1319 NZM Broth, 1319 NZY Agar. 1319 NZY Broth, 1319 NZYM Broth. 1319 G

0157:117 ID Agar, 1320 0157:117(+) Plating Medium, 1319 OA, 1321 Oak wilt fungi. 51.1320 Oak Wilt Fungus Hi Veg Agar. 1320 OA-EUP. 1322 Oat Flake Medium, 1320 Oat Makes Agar, 1320 Oat Makes Agar with Yeast Extract. 1320 Oat Meal Agar with Lupine Stems. 1322 Oatmeal Agar. 885. 1321 Oatmeal Agar A, 1321, 1322 Oatmeal Agar B. 1321,1322 Oatmeal Agar with Eupinc Stems. 1322 Oauneal Nitrate Agar. 1322 Oatmeal Salts Agar. 1323 Oatmeal Soy Peptone Medium, 1323 OAY Agar. 1323 ()l>es, 939, 963 Obligate anaerobes. 1773, 1775 Obolunna ilryuphila, 1199 Oceanilhermus Medium.. 1323 Oceanithemms profundus. 1324 Oceanithermus profundus Medium, 1324 Oceanithermus species. 1324 Oceanospiriilum beijerinckii, 1018 Oceanospiriilum jannaschii. 1558 Oceanospiriilum japontcvm, 1009 Oceanospiriilum maris, 1366 Oceanospiriilum minutulum. 1009 Oceanospiriilum multiglobuliferum, 1018 Oceanospiriilum pusillum. 1009, 1018 Oceamtspirillum species, 1009 Ochrobactrum anthropi. 1571 Ochromonas malhamensis, 1325 Ochromonas Medium, 1325 OCLA, 258 Oclosporomyces octosporus, 1909 Oddoux Medium, 1325 Odontia bicolor, 775 Odontiauda, 1000 Oerskovia species, 767, 1330 Oerskovia turbata. 768.1587 Oerskovia xanthineolylica, 1587

Index

OF Basal I liVeg Medium with Carbohydrate, 1325 OF Glucose Medium, Semisolid, 1336 OF Glucose Medium, Semisolid with NaCl. 1336 OF Medium. 1335. 1336 OF Test Medium, 1336 Ogawa Fgg Medium, 1326 Ogawa TB Medium, 1326 OGYK Agar, 1337 OGYK Agar Base, 1336 (XJYH Agar Base IliCrome, 842 Oidiotlendron c-erealis, 1000 Oidiodendron cltlamydosporicum. 10(X> Oidiodendron Jlavtmt, 1000 Oidiodendron griseum, 1000 Oidiodendron periconioides, 1000 Oidiodendron tenuissimum. 1000 Oikomonas species, 1016 Oil Agar Medium. 1326 Oleic Albumin Complex. 5, 1327 Oleiphilus messinensis. 1328 Oleispira anlarclica, 1328 Oligella ureolytica. 721 Oligella urelhralis, 215. 721 Oligotropha carboxidovorans, 315 Olinella succinogenes. 372 Olsenella nli. 157 OM-2 Medium, 1327 ONE Broth-/.i*teha, 1327 O-nitrophcnol utilizing bacteria, 1180 Onbz Salmonella Agar. 1327 ONTO Broth, 1327 ONR7a Medium, 1327, 1328 Onygena cor\>ina. 706 Onygena equina. 706 Oocystix species, 70 Opegrapha lichenoides, 951 Ophiobolus species. 638 Ophiocyiium majus, 70 OpUufus sp. DSM 14424, 1210 Opilutus terrae, 1347 OR Indicator Agar. 1328 Oral cavity, 1485 Oral J-'usobacterium Medium, 1328 Oral streptococci. 1892 Oral Treponema Medium, 1329 Oral treponemes, 1451, 1809, 1810, 1811, 1812 Orange Serum Agar, 1329 Orange Serum Broth Concentrate, 10X, 1329 Orange Serum HiVeg Agar, 1329 Orange Scrum EliVcg Broth. 1330 Orhicula parietina, 944 Organic Acid Medium KP, 1330 Organic Acid Medium. Kauffmann and Petersen, 1330 Organic acid production. 1646 Organic Medium. 79. 1330 Ornithine Broth, 1330 Ornithine Broth with Sodium Chloride. 1330

2<xw

Ornithine decarboxylase. 977, 1191. 1220, 1223,1514. 1520. 1565 Ornithine decarboxylation, 488,1330 Ornithinicoccus hortensis. 1513 Ornithinimicrobium humiphilum. 1513 Ornithobaclerium rhinolracheale, 1830 Orotic Acid Medium, 1331 OS Medium, 1331 OS Solid Medium, 1331 Oscillatoria species, 213, 214 Osmophilic bacteria, 1257,1312,1787 Osmophilic fungi, 481. 1056, 1257. 1787 Osmophilic Fungi Medium. 1332 Osmophilic yeasts. 742. 1257 Osmophilicbactcria, 742 Osmophyllic Agar, 1331 Osmotic stabilization. 1900 Osmotolerant microorganisms. 1003 OSrt Broth, 1332 OH Medium, Modified, 1332 Ottow Medium. 1332 Oitow's Agar Medium. 1332 Ottow's Medium, 1332 Oxacillin Resistance Screening Agar Base.

Oxytetracycline Glucose Yeast Fxtract Agar, 1337 Oxytetracycline Glucose Yeast Extract Agar IliCrome. 842 Oxytetracycline OYE Supplement, 7 Oysters. 914 OZR Medium. 1337

1332 Oxacillin-resistant microorganisms, 1333 Oxalate Maintenance Medium. 1333 Oxalate Medium. 1333 Oxalate Medium, Modified, 1333 Oxalate utilization, 1334 Oxalate Utilization Medium, 1333 Oxalate-decomposing Alcaligenes species. 1333 Oxalobacler/onnigenes, 1333.1334 Oxalobacter Medium. 1334 Oxalobacler vibrioformis, 107 Oxford Agar, 1334 Oxford Agar. Modified, 1216.1334 Oxford Anlimicrobic Supplement, 7 Oxford Medium. 1335 Oxgall, 7 Oxidation-Fermentation Medium. 1335 Oxidation-Fermentation Medium. I lughI Wilson's, 1335 Oxidation-Fermentation Medium, King's, 900,1335 Oxidation-Reduction Indicator Agar. 1328 Oxidativc-Fermentativc Glucose Medium, Semisolid. 1336 Oxidativc-Fermentativc Glucose Medium. Semisolid, with Sodium Chloride, 1336 Oxidativc-Fermentativc Medium. 1336 Oxidativc-Fermentativc Test Medium. 1336 Oxoid Chromogenic Listeria Agar, 258 OxoidCM3,1303 Oxoid Novel Hnrichment (ONH) Broth-/./v/eria, 1327 Oxoid Salmonella Rapid Test, 1536 Oxytetra Glucose Yeast Agar Base, 1336 Oxytctra Glucose Yeast Agar Base with Biotm, 1337

Pai Medium, 1339

P Agar. 1337 P-2 Medium, 1338 PA Agar, 1439 P-A Broth, 1426 PA HiVeg Broth. 1338 Pablum Cereal Agar, 1338 PA-C Agar, 1338 Packer's Agar, 162 Paecilomyces fumosoroseusy 1418 Paembacillus aiginolylicus, 1309 Paenibacillus chondroitinus. 1309 Paenibacillus lentimorbus, 1841 Paenibacillus species, 441, 1178, 1316 Pagano Levin Agar, 1338 Pages Balanced Salt Solution. 1339 PAI.CAM,955 PAI .CAM Agar, 1339. 1340 PAI.CAM Listeria Selective Agar, 1340 PAI .CAM Selective Supplement, 7 Palleroni and Doudoroff Mineral Base Agar, Modified, 1340 Panaeolus cyanescens, 613 Pandoraea species, 1830 Pannonibacler phragmitetus, 853 Panthenol assay, 1342 Panthenol Assay Medium, 1342 Pantothenate assay. 1341 Pantothenate Assay HiVeg Medium, 1340 Pantothenate Assay Medium, 1340, 1341 Pantothenate Culture Agar, USP. 1341 Pantothenate Inoculum HiVeg Broth, 1341 Pantothenate Medium, AOAC USP, 1341 Papillibacter cinnamiwrans: 1343 Papillibacter Medium. 1342 Paracoccidioides brasiliensis, 251 Paracoccus alcaliphilns, 1112. 1113, 1343 Paracoccus alcaliphilns Medium. 1343 Paracoccus aminophilu. 1343 Paracoccus aminophilus/Paracoccus aminovorans Medium, 1343 Paracoccus aminovorans, 1343 Paracoccus denitrificans. 1179, 1785 Paracoccus hafodeni/rificans, 229. 1311. 1314, 1343,1344 Paracoccus halodeniirificans Agar, 1343 Paracoccus kocurii, 1698. 1796 Paracoccus kocurii Medium, 1344 Paracoccus solventiwrans, 507 Paracoccus species. 1112 Paracoccus ihiocyanatus, 287 Paraffin Agar, 1344

2010

Index

Paraffin Medium with McClung CarbonFree Broth, 1344 Parajlabellula reniformis. 707 Paramecium Medium, 1344 Paramecium species. 1345,1589 Paramoeba pemaquidensis, 1011 Paranophrys species. 989 Parasporohactehum paucivorans DSM 15970, 1213 Paratetramilus jugosus, 1281, 1454 Paratyphoid. 1392 Parauronema acutum, 989 Park and Sanders Fnrichment Broth, 1345 Park and Sanders Fnrichment 1 liVeg BroUt Base with I lorse Blood and Selective Antibiotics. 1345 Parmelia centrifuga, 951 Parmelia consjwrsa. 951 Paromomycin Vancomycin Blood Agar, 1450 Parvopolyspora pallida. 1929 Pasteurella avium, 251, 774 Pasteurella haemolyttca. 306. 1346 Pasteurella haemnlytica Selective Medium, 1346 Pasteurella mult<Kida,7>Q(\ 1346, 1949 Pasteurella multocida Selective Medium. 1346 Pasteurella piscicida, 1554 Pasteurella species, 1833 Pasteurella tularensis, 744 Pasteurella volantinum. 774 Pasteurella volantium, 721 I'asteuria ramusa. 1458 Pathogenic fungi, 497, 1204, 1329, 1330 Pathogenic staphylococci. 280,1007 Pathogens, 374 Patients. 941. 1164. 1165. 1166 Paucimonas lemoignei, 865, 1832 Patfova luthen, 822 Paxillus alromentosus, 1199 Payne, Scghal. and Gibbons Medium, 1346 PB90-1 Medium, 1347 PBS, 1339 PC A, 1347 PCA+T+S, 1348 PD2 Medium. 1348 PD3 Agar. 1348 PDA Agar. 1412 PDA and Yeast Medium. 1414 PD-AI.S (Pierce's disease-almond leaf scorch) bacteria, 888, 1348 PDA-LUP. 1348 PDAmbAgar, 1413 PDM-114 Medium. 1348 PDYAgar, 1415 PF2 HiVeg Medium, 1349 PF2 Medium, 1349 Pea Agar Medium. 1350 Pectate degrading enzymes. 1407 Pectale lyase. 1228 Pectin Agar. 1350

Pectin degrading. 1563 Pectin Medium, 1350 Pectinase, 1350 Pectinatus cerevisiiphilus, 920, 1456 Pectinatus frisingensis. 1456, 1890 Pectinatus species, 1236 Pectinolytic bacteria, 76.464, 695 Pectinotrichum llanense, 706, 944 Pectobacterium carotovorwn Medium. 1350 Pectohacterium carolovorum sul)Sp. odoriferum, 1350 Pediaslrum tetras. 70 Pediococcus acidilacli ATCC 8042, 91 Pediococcus acidilactici. 344,768,922.1053 Pediococcus cereviseae and Aeixwoccus \iridans Medium. 1351 Pediococcus damnosus, 918, 1351 Pediococcus damnosus Medium, 1351 Pediococcus halophilus, 253, 748, 769, 1584 Pediococcus halophilus Medium. 1351 PeditK-occus Medium, 1351 Pediococcus Medium with Mevalonic Acid. 1351 Pediococcus pentosaceus, 768, 1235 Pediococcus species, 22.718. 748.920.1801 Pediococcus urinaeequi, 1429, 1830 Pediocococats cere\iscac, 1351 Pedohacter species. 1830 Pedomicrobium americanum. 1352, 1353 Pedomicrobium ferrugineum, 1352 Pedomicrobium PSM Medium, 1351 Pedomicrobium 1*SM Medium with Ribose. 1352 Pedomicrobium PYVM Medium, 1352,1353 Pedomicrobium species, 330, 1353 Pelczaria aurantia, 215 Pelistega europaea, 1830 Pelol>acter acelylenicus, 1354 Pelobacter acetylenicus Medium, 1353 Pelobacter acidigallici, 1354, 1356 Pelobacter acidigallici Medium, 1354 Pelobacter carbinolicus, 1355 Pelobacter carbinolicus Medium, 1354 Pelobacter massiliensis, 1356. 1668 Pelobacter Medium. 1355 Pelobacter Medium with Gallic Acid. 1356 Pelobacter propionicus, 1357 Pelobacter propionicus Medium. 1356. 1357 Pelobacter species. 1798 Pelobacter venetianui, 1358, 1359 Pelobacter wnetianus Marine Medium, 1357 Pelobacter venetianus Medium, 1358 Pelotlictyon luteolum, 1380 Pelodictyon phaeum, 1380 Petotomaadum Medium. 1359, 1360 Pelotomaadum thermopropionicum, 1360 Pclotomaculum therniopropionicum DSM 13752, 1360 PKM, 1426 PI-MBA, 1406 Pcnassay Base Agar. 131 Penassay Broth, 131

Penassay Broth with Chloramphenicol, 1361 Pcnassay Broth with Magnesium, 1361 Penassay Glucose Thymine Medium. 1361 Penassay G-THY Medium, 1361 Penassay Seed Agar. 130 Penicillin. 1776, 1779 Penicillinasc-Producing Neisseria gonorrhoeae Medium, 1425 PenicUlium aurantiogriseum. 1361 Penicillium chrysogenum, 930 PenicUlium citreonigrunu 479 Penicillium crustosum. 952 PenicUlium cyclopium, 1361 Penicillium isariiforme. 988 Penicillium ochr<K-hloron, 944,988 Penicillium pinophiium, 944. 988 Penicillium species. 459, 755, 1002,1189, 1902 Penicillium spinuhysunK 1000, 1001 Penicillium thomii. 479 Penicillium verruculosum, 1001 Penicillium viridicatium. 1361 Pentachloronitrobenzene Rose Bengal Yeast Fxlracl Sucrose Agar, 1361 Pentachlorophenol Medium, 1361 Pcntachlorphenol utilizing bacteria. 1180 Pentatrichomonus hominis. 1861, 1862

PEP Medium. 1362 Pept Carb Soluble Starch Agar, 1362 Peptamin, 2 Pcptcd Meat Broth. 1038 Peptococcus aerogenes, 1370 Peplococcus gtycinophilus. 1363 Peptococcus glycinophilus Medium, 1362, 1363 Peptococcus Medium, 1363 Peptococcus niger, 366 Peptococcus species, 1550 Peptone Broth, 1363 Peptone Carbonate Starch Agar. 1362 Peptone Cholic Acid Recovery, 1363 Peptone Com Agar, 1363 Peptone Fumarale Sulfate Medium. 1381 Peptone Glucose Liver Extract Medium. 1382 Peptone Glucose Salt Agar, 1382 Peptone Glucose Yeast Fxtract Agar, 1382 Peptone Glycerol Phosphate Broth. 1382 Peptone Iron Agar, 1364 Peptone Iron Hi Veg Agar. 1364 Peptone Meat lixtract Glycerol Agar. 1364 Peptone Meat Fxtract Soil Fxtract Agar. 1364 Peptone Medium. 1364 Peptone Recovery Broth, 1365 Peptone Sodium Cholate, 1365 Peptone Sorbitol Bile Broth, 1365 Peptone Sorbitol HiVeg Broth, 1365 Peptone Starch Carbonate Medium. 1365 Peptone Starch Dextrose Agar, 1365 Peptone Succinate Agar, 1365. 1366 Peptone Succinate Agar in Sea Water, 1366

Index

Peptone Peptone Peptone Peptone

Succinate Salts Broth, 1366 Succinate Salts in Sea Water. 1366 Succinate Salts Medium, 1366 Sucrose Broth. 1367

Peptone Water, 1367 Peptone Water with Andrade's Indicator, 1367 Peptone Water, HiVeg, 1367 Peptone Yeast lixtract, 1% Medium, 1452 Peptone Yeast lixtract Agar, 1367. 1368 Peptone Yeast Extract Carboxymcthyl Cellulose Medium, 1451 Peptone Yeast lixtract Glucose Agar. 1368 Peptone Yeast lixtract Glucose Agar with Casein, 1368 Peptone Yeast lixtract Glucose Broth, 1457 Peptone Yeast lixtract Glucose Maltose Medium, 1460 Peptone Yeast I-ixtract Glucose Medium. 1457, 1458 Peptone Yeast Extract Glucose Medium lor Spirillum. 1459 Peptone Yeast lixtract Glucose Medium, Modified, 1368 Peptone Yeast lixtract Glucose Salt Medium. 1456 Peptone Yeast lixtract Glucose Vitamin Marine Medium, 1461 Peptone Yeast Extract Glucose Vitamin Medium, 1462 Peptone Yeast lixtract Inositol Medium, 1451 Peptone Yeast lixtract Iron Agar, 886 Peptone Yeast lixtract Iron Hi Veg Agar. 884 Peptone Yeast lixtract Medium. 1369 Peptone Yeast lixtract Medium with Fructosc. 1452 Peptone Yeast lixtract Medium with Glucose. 1453 Peptone Yeast lixtract Salt Agar. 1469 Peptone Yeast lixtract Salt Medium. 1454 Peptone Yeast Glucose Seawaler Agar, 1461 Peptone Yeast Glutamatc Medium. 1370 Peptone Yeast Medium with Magnesium Sulfate. 1370 Peptone Yeast Medium with Magnesium Sulfate. 1370 Peptone Yeast Tryplicaseâ&#x201E;˘ Agar. 1370 Peptone, C/apek's, 1363 Peptone-Reduced Agar, 1365 Peptones, 2 Peptonized Milk Agar. 1370 Peptostreptococcus anaerobius. 1459. 1890 Peptoxtreptococcus asaccharalyticus, 1890 Peptostreptococcus heliotrinreducens, 1846, 1862 Peptostreptococcus hydrogenalis. 718 Peptostreptococcus indolicus, 1459, 1890 Peptostreptococcus magnus, 447.448. 1459. 1890 Peptostreptococcus micros, 368, 1363, 1890 Peptostreptococcusprevotii, 718, 1459, 1890

Peptostreptococcus productus. 366, 718. 1459 Peptostreptococcus species, 369, 371. 631. 1459,1550 Peptostreptococcus tetradius, 718. 1459. 1890 Percohmonas cosmopolitus, 1556 Perfringens Agar, OPSP, 417 Pcrfringcns HiVcg Agar Base (O.P.S.P.) with Antibiotics. 1381 Perfringens Hi Veg Agar Base with ligg Yolk and Antibiotics. 1371 Perfringens OPSP Selective Supplement A, 7 Perfringens SIP Selective Supplement A, 7 Perfringens ISC Selective Supplement A,7 Perkinsus Agar Medium. 1371 Perkinsus andrewsi, 1372 Perkinsus atlonticus. 1372 Perkinsus chesapeaki, 1372 Perkinsus marinus. 1372 Perkinsus Medium, 1372 PersejriioneUa hydrogeniphila, 852 Persephonella Medium. 1373 Persephonella species, 1373 Persicohticter difjluens, All Pctragnani Medium, 1373 Petrobacter succinimandens\ 738 Petrotoga Medium, 1373, 1374 Pelrotoga miothenna, 1374 Petrotoga olearia, 1576 Petrotoga sibinca, 1576 Pfennig's Medium, 2 Modified for Green Sulfur Bacteria, 1379 Pfennig's Medium I, Modified for Marine Purple Sulfur Bacteria. 1374 Pfennig's Medium I, Modified for Purple Sulfur Bacteria, 1375 Pfennig's Medium II Modified for Green Sulfur Bacteria, 1045 Pfennig's Medium, 1 with 1% Salt. 1376 Pfennig's Medium, 1 with 3% Salt, 1377 Pfennig's Medium, 1 with Yeast Extract, 1378 Pfennig's Medium. 1, Modified for Marine Purple Sulfur Bacteria, 1375 Pfennig's Medium. 2 with Salt, 1380 Pfennig's Medium, 2, Modified tor Green Sulfur Bacteria. 1378 Pfizer Selective linterococcus Agar, 1380 Pfizer Selective Enterococcus HiVeg Agar. 1381 Pfizer IB Medium Base with Glycerol, ligg Yolk. Glucose, and Malachite Green. 1381 PFS Medium. 1381 PGA, 1382 PGI.Ii Medium. 1382 PGP Broth, 1382 PGS Agar. 1382 PGI Medium. 1382 PGY Agar. 1382 pH Buffers, 8

2011

pi I indicators, 7 Phaeolepiota aurea, 1199 Phaffia rhodozyma. 1941, 1942 Pharmaceutical, 124, 128, 129,133, 171, 172.698.1258.1341,1533 Pharmaceutical preparations, 748 PUB Delalleld Agar. 1383 PI IB Medium. 1406 PHB/Pyruvatc Medium. 1383 PI IC Medium, 1384 PheUinus igniarius, 348. 472 PheUinus nigricans, 472 PheUinus pomaceus, 775 PheUinus fremiti us, 775 PheUinus weirii, 775 Phenanthrene utilizing bacteria, 1056 Phenethyl Alcohol Agar, 1384 Phenol coefficients, 135.602, 1671 Phenol Nutricnt-Su|>plemented Broth, 1384 Phenol Red Adonitol BroUi. 1384 Phenol Red Agar, 1385 Phenol Red Arabinose Broth. 1385 Phenol Red Broth, 1385 Phenol Red Broth Base with Plant lixtract No. 1,1385 Phenol Ret! Carbohydrate Broth. 1386 Phenol Red Carbohydrate BroUi with Sodium Chloride, 1386 Phenol Red Dextrose Broth, 1386 Phenol Red Dulcitol HiVeg Broth, 1386 Phenol Red ligg Yolk Polymyxin Agar Base. HiVeg, 1271 Phenol Ret! Galactose Broth. 1387 Phenol Red Glucose Broth, 1387 Phenol Red Glucose Broth with Sodium Chloride. 1387 Phenol Red Glucose Broth, HiVeg, 1387 Phenol Red Glucose HiVeg Agar, 1387 Phenol Red I liVeg Agar Base with Carbohydrate, 1388 Phenol Red Hi Veg Broth Base. 1388 Phenol Red Inositol Broth. 1388 Phenol Red Lactose Agar, 1388 Phenol Red lactose Broth. 1388. 1389 Phenol Red Lactose HiVeg Agar. 1389 Phenol Red lactose HiVeg Broth, 1389 Phenol Red Maltose Broth, 1389 Phenol Red Maltose HiVeg Agar. 1389 Phenol Red Maltose HiVeg Broth, 1389 Phenol Red Mannitol Agar, 1390 Phenol Red Mannitol Broth, 1390 Phenol Red Mannitol HiVeg Agar, 1390 Phenol Red Mannitol HiVcg Broth. 1390 Phenol Red Rallinose Broth, 1390 Phenol Red Rhamnose Broth. 1390 Phenol Red Salicin Broth, 1391 Phenol Red Sorbitol Broth, 1391 Phenol Red Staich Broth, 1391 Phenol Red Sucrose Broth. 1391 Phenol Red Sucrose HiVeg Agar. 1391 Phenol Red Sucrose HiVeg Broth, 1391 Phenol Red Tartrate Agar, 1392

2012

Index

Phenol Red Tartrate Broth, 1392 Phenol Red Tartrate HiVeg Agar, 1392 Phenol Red Trehalose Broth, 1392 Phenol Red Xylose Broth, 1392 Phenolphthalein Phosphate IliVeg Agar, 1392 Phenylacetic acid utilization. 1181 Phenylalanine Agar, 1392 Phenylalanine Deaminase Medium, 1392 Phenylalanine Malonate Broth, 1393 Phenylalanine production. 1393 Phenylethanol Agar. 1384. 1393 Phenylethanol Blood Agar, 1393 Phenylethyl Alcohol Agar, 1384 Phenylethyl Alcohol IliVeg Agar, 1393 Phenylketonuria, 1400 Phenylketonuria Test Agar, 1400 Phenylobacterium Agar, 1393 Phenyhhacterium conjiinclum, 1455 Phenylobacterium immobile, 1172. 1174, 1394 Phenylobacterium lituiforme, 1519 Phenylobacterium Medium, 1394 Phialophora gregata, 898 Phingomonas chlorophenolica, 826 Phisica millegrana, 951 Phisica stellaris. 951 Phlehia chrysocrea, 472 Phlebia gigantea. 775 Phlebia lirida, 472 Phleospora idahoensis. 952 Phlyclochylrium africanum, 746 Pholiolu lenta. 1065 Phoma chiysanlhemi, 1410 Phoma eupyrena. 952, 1410 Phoma exigua, 1065,1410 Phoma foveata, 1410 Phoma glomerata, 615, 1000 Phoma leveillei, 348 Phoma lingam, 952,1410 Phoma iini. 615 Phoma macrostoma, 1410 Phoma medicaginis, 1350 Phoma pituxlella. 1410 Phoma putaminum, 1410 Phomopsis occulta. 317 Phormidium species, 213,214, 1200 Phosphatase. 1392 Phosphate Mineral Salts Medium with Octane. 1394 Phosphate solubilizing microorganisms, 1397 Phospholipases, 940 Phoiobacteria. 1395 Photobacterium Agar, 1394 Photobacterium Broth, 1394, 1395 Photobacterium IliVeg Broth. 1395 Photobacterium leiognathi, 1395 Photobacterium Medium. 1395 Photobacterium MPY Medium, 1395 Photobacterium phosphoreum. 1395. 1554 Photobacterium sj>ecies, 753, 971

Pholorhabdus luminesce/is, 1484 Phrealamoeba balamuthi, 1861 Phthalic Acid Medium. 1395 p-\ lydroxybenzoate Agar, 863 />Hydroxybenzoate utilization, 863 PI IYG Medium, 1396 Phyllobacterium frifolii, 1947 Phyllosticla sojaecola, 952 Phymatotrichum Medium, 1396 Phymatotrichum omnivorum, 1396 Physalospora tucumanensis, 988 Physarum polycephalum. 1272 Phytotnonas davidi, 461, 1217 Phytoneâ&#x201E;˘ peptone, 3 Phytoneâ&#x201E;˘ Yeast Hxlract Agar. 1396 Phytopathological fungi, 450,952 Phytophthora cactorum. 898, 1348 Phytophthora cinnamomi, 1877 Phytophthora citrophthora. 267, 1348, 1878 Phytophthora cryptogea. 898, 1877 Phytophthora drechsleri, 1878 Phytophthora erythroseptica. 898, 1878 Phytophthora fragariae, 898 Phyto})hthora heveae, 898 Phytophthora infestans, 1878 Phytophthora megasperma. 1032 Phytophthora nicotianae. 1348. 1878 Phytophthora jyalmivora, 1878 Phytophthora species, 452, 952 Phytophthora syringae. 898. 1878 Pichia angusta. 1935, 1940 Pichia membranae/aciens, 589 Pichia species, 1001,1941, 1942. 1949 Picrophilus Medium, 1396 PicrophUus oshimae. 1397 Picrophilus torndus, 1397 Pigment production. 900.962.963. 1261. 1440, 1477 Pike Streptococcal Broth, 1397 Pike Streptococcal 1 IiVeg Broth Base with BlOOd, 1397 Pikovskayas Agar, 1397 Pilimelia anulata. 481. 1364. 1575 Piiimelia species, 1364 Pilimelia lerevasa, 481, 1364 Pilobolus Agar. 1397 Pilobolus Medium, 1397 Pilobolus species. 1397 Pdobolus sphaerosporus, 1398 PIM. 1438 Pine and Drouhefs Ili.stopla.sma Yeast Phase Medium, 1398 Ptrellula marina, 985, 1399 Pirellula marina Medium, M14, 1399 Pirellula staleyi. 330 Pisolithus tinctoruis, 1199 Pislillaria micans. 392 PLslillaria setipe.s, 392 Pisu Medium. 1399 Pityrosponun Agar, 1399 PityrosfMM-um Medium. 1399 Pityrosporum Medium II, 1400

Pityrosporum ovale. 450. 639, 1400 Pityrosporum species, 1399 Pi/i/en Potato Agar, 1611 PKU, 1400 PKU lest Agar, 1400 PL Agar, 1400 Pl.HiVegAgar. 1401 Plaice Medium, 1401 Planctomyces brasiliensis. 1462 Planctomyces limnophilus, 1461, 1462 Planctomyces Medium, 1401 Planctomyces species, 1401 Planklonic gas-vaeuolate cyanobacteria, 1900 Planohispora longispora, 1322, 1411 Pianobispora rosea, 753, 1517, 1939 Planococcus citreus, 1311, 1554 Ptanococcus kocurii. 454, 1554 Planococcus species, 117, 798, 1554, 1857 Planococcuskocurii, 1558 Planomonospora parontospora, 481, 1322 Planomonospora venezueiensis. 1411. 1939 Plant Mycoplasma Agar, 1401 Plant Mycoplasma Broth, 1402 Plants, 1863 Plaque. 892.1197 Plasmid, 1696 Plasmids, 1696 Plasimxlium falciparum. 1519 Plastics, 155 Plate Count Agar. 1402, 1403 Plate Count Agar with Antibiotic, 1403 Plate Count Agar with Antibiotic-l;ree Skim Milk, 1403 Plate Count Agar. HiVcg. 1403 Plate Count Agar, Modified, 1403 Plate Count Agar. Special, 1403 Plate Count Broth, 1403 Plate Count HiVeg Agar. 1404 Plate count method, 1403, 1412 Plate Count Ml JO Agar, 1404 Plates with Fluoranthcnc. 1404 Plectomena boryanum, 388 Plectosphaerella cucumerina. 589 Pleisomonas shigelloides, 1401 Plesiocystis pacifica. 1899 Plesiomonas Differential Agar, 877 Plesiomouas Differential HiVcg Agar. 877 Plesiomonas shigelloides. 877, 1484 Plesiomonas species. 877, 1514, 1515, 1538 Pl.KT Agar, 1404 Pteurocapsa species. 213. 214. 1200 Plunkellomyves litloralis, 706 Plulon Medium, 1405 PM Indicator Agar, 62. 1405 PMA Medium, 1370 PMP Broth, 1405 PmTOAgar, 1405 PMY Medium. 1405 PMYA II Medium, 1405 Pneumococci. 230. 244.466,1851 Pneumonia, 1268, 1305,1421, 1423

Index

Polluted waters, 158. 330, 1463, 1648. 1938 Polyangium Agar. 1406 Polyangium hrachysporum, 1594 Polyangium eellulosiun, 1406, 1589 Poly-p-Hydroxybutyrate Medium, 1406 Pofyedriella helvetica. 70 Polygalactana.se, 1228 Polyhexametliylene Carbonate Medium, 1384 Polymyxin Acriflavine I,iCl Ceftazidime Fsculin Mannitol Agar. 1339, 1340 Polymyxin Base Agar, 123, 132 Polymyxin HiVeg Base Agar, 127 Polymyxin Pyruvate Fgg Yolk Mannitol Bromthymol Blue Agar, 1406 Polymyxin Seed Agar, 123, 132 Polymyxin Seed HiVeg Agar, 128 Polymyxin Staphylococcus Medium. 1406 Poiypaecilum pisce, 988, 999 Polypectate Gel Medium. 1407 Polypcptonc, 3 Polysaccharide. 1653 Polysaccharide production, 1652 Polyscytalum pustulans, 1410 Polysorbate, 80 Agar, 1407 Polysorbate. 80 HiVeg Agar, 1407 Potysphondylium pallidum, 930 Polysphondylium violaceum, 930, 952 Polyloma auomale, 1588 Polytoma difficile, 1588 Polytoma ellipticum, 1588 Polytoma mirum, 1588 Polytoma species, 860 Polytoma uvella. 1588 Polytomella caeca, 860 Polytomella parva, 860 Pontccorvo's Aspergillus Medium, 1407 Pontccorvo's As/>etgillus Medium with 0.05% Yeast Extract, 1407 Porcine Heart Agar. 1407 Poria medulapanis. 775 Porina satulwichetviis, 951 Pork Plasma I'ibrinogen Overlay Agar, 1408 Porphyridium purpureum, 85, 156 Potphyrohacter cryptus, 1409 Porphyrohacter species, 1409 Porphyrohacter tepidarius, 1409 Porphyrohacter tepidurius Medium, 1408 Porj)hyromouas gingiwdis, 370 Porphyromonas species. 371 Poslgate's Medium, 1409 Postgate's Medium B lor Sulfate Reducers, 1042 Postgate's Medium C for Sulfate Reducers, 1043 Postgate's Medium D for Sulfate Reducers, 1046 Postgate's Medium F for Sulfate Reducers. 1048 Postgate's Medium F for Sulfate Reducers, 1050

Postgate's Medium for Sulfate Reducers, 1062 Postgate's Medium G for Sulfate Reducers. 1051 Postgate's Medium N for Sulfate Reducers. 1055 Potable samples. 1927. 1929 Potassium Cyanide Broth, 1409 Potassium Cyanide HiVeg Broth Base with KCN, 1409 Potassium Tellurite Agar, 1410 Potato Agar. 1410 Potato Agar with Cereal Culms, 1410 Potato Carrot Agar, 1410 Potato Carrot Agar, Diluted, 1/10, 1410 Potato Agar with Mangancscl411 Potato Carrot Broth, 1411 Potato Carrot Medium, 1411 Potato Dextrose Adenine Agar, 1411 Potato Dextrose Agar, 1412 Potato Dextrose Agar and Yeast Medium, 1414 Potato Dextrose Agar wiUi 2% Glucose and 60% Sucrose. 1413 Potato Dextrose Agar with 7.5% Sodium Chloride, 1414 Potato Dextrose Agar with Antibiotics, 1413 Potato Dextrose Agar with Gentamicin, 1413 Potato Dextrose Agar with Methionine and Biotm, 1413 Potato Dextrose Agar with Thiamine, 1414 Potato Dextrose Agar, 1/3 Strength. 1414 Potato Dextrose Agar. 1/4 Strength. 1414 Potato Dextrose Agar, pH 5.0,1413 Potato Dextrose Agar. Quarter Strength, 1414 Potato Dextrose Broth, 1415 Potato Dextrose Broth with Yeast Fxtract. 1415 Potato Dextrose L-IsolcucineAgar. 1415 Potato Dextrose Salt Agar. 1415 Potato Dextrose Yeast Agar, 1415 Potato Extract Agar, 1416 Potato Flakes Agar. 1416 Potato Glucose Agar. 1416 Potato Infusion Agar, 1416 Potato Infusion HiVeg Agar. 1416 Potato Infusion with Inorganic Salts, 1416 Potato Malt Agar, 1417 Potato Malt Agar with Filler Paper. 1417 Potato Malt HiVeg Agar. 1426 Potato Medium, 1417 Potato P-YH Thermus Medium. 1417 Potato Sucrose Agar, 1417,1438 Potato Yeast Agar. 1418 Potato-Glucose Agar, 1382 Potomacus pottsi. 1419 Pour plate, 1472, 1473 Pour plate technique, 1886 Powell and Frrington's Medium, 1418 PP Agar. 1418 PP Medium, 1418

2013

PP Starch Medium. 1419 PPB, Modified Caldwell and Bryant, 1419 PPFS II Medium, 1420 PPFS-II Agar Medium,, 1419 PPG A Medium. 1420 PPI.OAgar. 1420. 1421 PPl.O Agar Base. 1263 PPI.O Agar with Additives for Mycoplasma, 1421 PPI.O Agar, pi I 7.6 with Additives for Mycoplasma. 1421 PPI.O Broth, 1422 PPI.O Broth Base without Crystal Violet, 1266 PPI.O Broth with Additives for Mycoplasma, 1422 PPI.O Broth with Bovine Serum. 1423 PPI.O Broth with Crystal Violet, 1423 PPI.O Broth with Penicillin, 1424 PPI.O Broth without Crystal Violet, 1423 PPI.O Broth without Crystal Violet with Horse Serum. 1424 PPI.O Broth without Crystal Violet with Horse Serum and Fresh Yeast Fxtract, 1423, 1424 PPI.O Broth without Crystal Violet with Horse Scrum. Glucose, and Fresh Yeast

Extract, 1424 PPI.O Broth without Crystal Violet with Sodium Acetate, Fresh Yeast Fxtract, and Calf Serum, 1424 PPI .O Broth pi 1 7.6 wiui Additives for Mycoplasma, 1422 PPI.O HiVeg Agar Base. 1267 PPI.O HiVeg Broth Base with CV, 1267 PPI.O HiVeg Broth Base without CV. 1268 PPI.O Serum Fraction, 5 PPI.Os. 1268. 1305. 1423 PPNG Selective Medium, 1425 PPT Agar. 1M. 1425 PPYG Medium. 1425 PRAS-PYG with Twccnâ&#x201E;˘. 80. 1425 Pre-Fni ichmeni HiVeg Broth Base with Magnesium Sulfate and Calcium Chloride. 1426 Preenrichmenl Medium, 1426 Preferred Medium, 1426 Preparation of Media, 8 Presence-Absence Broth. 1426 Piessure-temperauture relationships, 8 Preston Blood-Free Medium. 299 Preston Fnriehment Broth, 1427 Preston Fnriehment HiVeg Broth Base. 303 PiestOO HiVeg Agar Base with Horse Blood and Antibiotics. 1427 Preston's Campylobacter Medium, 308 Presumptive test, 649 Presumpto Media, 1427 Preussia typharum. 706 Prevolelia hivia, 1459 Prevolella huccae, 366, 1459 Prevolelia huccalis. 366, 1459

2014

Index

PrevoteHa denticoia. 366, 1459 Prevotella disiens, 370, 1459 PrevoteHa intermedia. 366. 718. 1459 Prevotella melaninogenica, 718 Prevotella oralis. 366 PrevoteHa ritminicola, 367, 616, 617 Prevotella species, 369, 371. 631 Prey Seawater Broth, 1427 Pril Xylose Ampicillin Agar. 1428 Pringsheim's Medium, 1428 Procryptobia species. 1015 Pmmicromonospora cilrea, 481,1321,1330, 1936 Promicromono.spora enterophila, 210, 767, 1330 Promicromonospora sukumoe, 1939 Propionibacterium acidipropionici, 616, 617,718.909, 1428.1429 Propionibacterium acnes, 1459, 1890 Propionibacterium Agar. 1428 Propionibacterium avidum, 1459, 1890 Propionibacterium freudenreichii. 909, 1429, 1459 Propionibacterium granulosum. 1459. 1890 Propionibacterium intermedium. 909 Propionibacterium jensenii. 909, 1429 Propionibacterium lymphophilum. 1459. 1890 Propionibacterium Medium. 1429 Propionibacterium propionicum, 1848 Propionibacterium propionicus, 215 Propionibacterium species, 56,57, 151, 369, 371, 631. 718, 909. 1585, 1586, 1801, 1836, 1931 Propionibacterium thoenii. 225. 909. 1428, 1429 Propionigenium maris. 1430 Propionigenium mans Medium. 1429 Propionigenium modestum, SIX 1430, 1431 Propionigenium modestum DSM 2376. 1431 Propionigenium modestum Medium, 1430, 1431 Propionispira arboris, 1432 Propionispira Medium, 1431 Propionispora Medium, 1432 Propionispora vibrioides. 1433 Propionivibrio dicarhoxylicus, 1434 Propionivibrio limicola. 757 Propionivibrio/Acetivibria'l'ormi\ibrio Medium, 1433. 1434 Proskauer-Beck Medium for Mycobacterium, 1435 Prosthecobacterfusifonnis. 759.1436 Prosthecohacter Medium, 1435 Proslhecochloris aestuarii. 1380 Prosthecomicmhium and Ancalomicrohium Medium, 1436 Prosthecomicrobium Prosthecomicrobium Prosthecomicrohium Prosthecomicrobium 1436

enhydrum, 1058, 1436 hirschii, 1058 litoralum, 243 pneuinaticum. 1058.

Prosthecomicrobium polyspheroidum. 1198 Prosthecomicrobium species, 1057, 1058, 1059 Prosthetic valve endocarditis, 941 Protacanthamoeba caledonica. 707 Protannnohacter thiammophaga, 1112 lÂťrotcolysis, 962. 963 Proteolytic activity, 1218, 1575, 1586 Proteolytic microorganisms, 292. 731.1631, 1632

Proteose Agar. 1436 Proteose 1 liVeg Agar, 1436 Proteose No. 3 Agar, 1436. 1437 Proteose peptone, 2 Proteose peptone, 3 Proteose peptone No. 2. 3 Proteose peptone No. 3, 3 Proteose Yeast Extract Medium. 1438 Proteromonas lacertae, 1861 Proteus mirabilis. 721, 1330.1881 Proteus rettgeri, 1330 Proteus species. 260. 261. 262.493.494. 845, 992,1407, 1409, 1410. 1537, 1558, 1625,1700,1869.1870 Proteus xtdgaris. 958, 1330 Protosiphon botryoides, 70 Protostelium irregularis. 809 Prototheca Isolation Agar, 1438 Prototheca morifonnis, 639. 1438 Prototheca stagnant. 639 Prototheca ulmea. 1438 Prototheca wickerhamii, 639 Prototheca zopfii. 639 Protozoa, 195 Provasoli Medium, 1438 Providencia species, 1919. 1920 Providencia stuartii, 1036, 1037 Prune Agar, 1438 PRY1-S Agar. 1361 PSA. 1438 PSD Agar, 1365 PSE Agar. 1380 Pseudanabaena sjKX'ies, 213, 214 Pseudeuroiuim desertorum. 706 Pseudeurotuim oudis. 706 Pseudoalteromonas atlantica. 1009 Pseudoalleromonas spiralis, 1439 Pseudoalteromonas spiralis Medium,. 1438 Pseudoamycolala halophobica. 1439 Pseudoamycolata hahmhobica Medium, 1439 Pseudoarachniotus roseus, 706 Pseudoarachniotus rulyer. 706 Pseudoarachniotus trochleosporus, 706 Psettdobodo tremulans, 1015 Pseudobutyrivibrio Medium, 1439 Pseudobutyrivibrio ruminis. 1439 Pseudocohnilembus marinus, 463 Pseudogymnoascus roseus. 944 Pseudohaloneclria adversaria, 638 Pseudohalonectria falcata. 638 Pseudohaloneclria phialidica, 638

Pseudomonadaceae. 1292 Pseudomonads, 690 Pseudomonas aeruginosa. 20, 21, 153, 154. 206, 285, 343, 344, 377,489, 644, 690, 6%. 845.854.895.958.997.1167.1168, 1274,1283, 1310, 1330,1338, 1440, 1441.1444.1552. 1561.1585.1950, 1953 Pseudomonas aeniginosa Agar, 1439 Pseudomonas Agar (for pyocyanin). HiVeg, 1440 Pseudomonas AgarF. 1440 Pseudomonas Agar P, 1440 Pseudomonas alcaligenes. 20, 1274 Pseudomonas amygdali. 1316. 1916 Pseudomonas anguilliseptica, 1314 Pseudomonas Asparagit>e Broth, 1440 Pseudomonas atlantica. 1009 Pseudomonas azotocolligaivi, 169 Pseudomonas Basal Mineral Medium, 1441 Pseudomonas bathycetes, 1441 Pseudomonas bathycetes Medium. 1441 Pseudomonas bei/ennckia. 1155 Pseudomonas beijerinckii, 799, 1311, 1538 Pseudomonas boreopolis. 1330 Pseudomonas butanomra, 1184 Pseudomonas carboxydohydrogena. 330, 1044 Pseudomonas carrageenovora. 1010. 1012 Pseudomonas caryophylli, 1416, 1904 Pseudomonas cepacia. 20.489. 690.1327, 13%, 1633,1834, 1930 Pseudomonas CFC Agar. 1441 Pseudomonas chloritidismutuns. 1442 Pseudomonas chloritidismutans Medium, 1441 Pseudomonas chlororaphis, 1184 Pseudomonas chlorotidismutans Medium, 1898 Pseudomonas CN Agar. 1442 Pseudomonas denitrificans, 690, 696, 748 Pseudomonas denitrificans Medium. 1442 Pseudomonas Deiiitrilication Medium, 1442 Pseudomonas echinoides. 643 Pseudomonas elongata, 95, 1441 PseudomonasJluorescens, 20,117,119.932. 997,998, 1414, 1444, 1446, 1468, 1880, 1928, 1942 Pseudomonas fragi. 1885 Pseudomonasfrederiksbergensis, 1051, 1056 Pseudomonas geniculata, 1307 Pseudomonas glathei, 748 Pseudomonas glumae, 900 Pseudomonas halophila, 1443 Pseudomonas halophila Medium. 1443 Pseudomonas IliVeg Agar Base, 1443 Pseudomonas indigofera. 1443. 1445 Pseudomonas indigofera Agar, 1443 Pseudomonas insueta, 1111. 1112 Pseudomonas Isolation Agar, 1443 Pseudomonas Isolation HiVeg Agar Base with Glycerol, 1444

Index

Pseudomonaa kmeeolata, 1938 Pseudomonas lemoignei, 1383, 1444 Pseudomonas lemoignei Agar, 1444

Pseudomonas UndbergH, 1412 Pseudomonas mallei. 752 Pseudomnnas maltophila, 1327 Pseudomonas Medium, 1444. 1445 Pseudomonas Medium A, 1445 Pseudomonas Medium B, 1445 Pseudomonas Medium I, 1445 Pseudomonas Medium No. 2.1446 Pseudomonas mendocina. 690, 696, 1362 Pseudomonas methanica, 912 Pseudomonas melhanotica, 1310, 1924 Pseudomonas iiautica. 1009 Pseudomonas oleovorans, 1394 Pseudomonas paucimobilis, 1051. 1056 Pseudomonas perlurida, 1330 Pseudomonas pertucinogena, 238 Pseudomonas Phage Medium, 1446 Pseudomonas phenazinium. 1306. 1484 Pseudomonas pickettii Medium, 1446 Pseudomonas polysaccharogenes. 1111 Pseudomonas pseudomallei, 752 Pseudomonas psueudoalcaligenes. 1943 Pseudomonas putida. 20,211,286,297,754, 864.879. 1005. 1179. 1181.1275. 1308. 1330, 1429, 1444, 1942 Pseudomonas saccharophila, 1446 Pseudomonas saccharophila Medium, 1446 Pseudomonas solanacearum. 709, 1446. 1579, 1653, 1863 Pseudomonas solanacearum Medium, 1446 Pseudomonas species. 19, 260. 261, 262. 283,285,291,314,451,493,494,594, 611.612, 691.695,696. 702, 713.740. 741, 743, 748, 749, 750, 754, 762,800, 802.812.814.856.874.900.1005,1040. 1044. 1139. 1168, 1170.1173.1176, 1180, 1183, 1185, 1304,1308, 1309, 1310.1312.1314.1318.1319.1363, 1402, 1406, 1425, 1441,1442, 1443, 144-4,1445.1446.1538.1554,1571. 1589. 1633, 1635, 1653.1690,1704, 1730, 1805, 1814, 1836, 1837, 1838. 1839, 1845, 1856, 1899,1926. 1942 Pseudomonas stutzeri. 1297, 1330. 1442 Pseudomonas syngii. 1446 Pseudomonas syngii Medium, 1446 Pseudomonas syringae, 1017, 1054, 1280, 1412.1416, 1447, 1592 Pseudomonas sy/ingae Selective Medium, 1447 Pseudomonas ihermocarboxydowrans, 1169 Pseudomonas toLuisii. 1313 Pseudomonas viscogena, 1111 Pseudomonas viscosa. 1885 Psetidonocardia compacta, 321, 767 Pseudonocardia species, 886 I'seudonocardta species, 768 Pseudonocardia (hennophila. 214,469. 638. 1363

Pseudosel*' Agar, 343 Pseudospirillum japonicum, 1009 Pseudotrichomonas keilini. 1859 P S S Broth. 1366 PSS Medium, 1018, 1366 PSTA Knriclimenl HiVeg Broth Base, 1447 Psychrobacter immobiiis. 643. 1446

Psychrohacler species, 1009 Psychrobacter urativorans. 1314 PsychroJIexus gondwanensts, 1009 Psychromonas antarctica. 1009

Psychrotrophic bacteria. 464.465 PT Agar, 1447 Pterulittsporu spinosisporti, 775 Pteridomonas danica, 1556 PI YG Medium, 1447 Purine fermenting, 411 Purple Agar. 1447 Purple Broth, 1448 Purple Broth with Sodium Chloride. 1448 Puq>le Carbohydrate Broth, 1448 Purple Carbohydrate Broth with NaCl, 1448 Purple Carbohydrate Fermentation Broth Base, 1448 Purple Carbohydrate Fermentation Broth Base with Hsculin. 1449 Purple IliVeg Agar Base with Carbohydrate, 1449 Purple I li Veg Broth Base with Carbohydrate, 1449 Purple Lactose Agar, 1449 Purple Serum Agar Base, 1449 Purple sulfur bacteria. 1375. 1376 PV Blood Agar, 1450 PW Medium. 1450 PXA Agar, 1428 PY. 1% Medium. 1452 PY Basal Medium. 1450 PY Broth, 1450 PY Carbohydrate Medium, 1451 PY CMC Medium, 1451 PY Inositol Medium, 1451 PY Medium, 1369 PY Medium with Fructose. 1452 PY Medium with Glucose, 1453 PY Medium with Scrum-Cocarboxylasc. 1453 PY Salt Medium. 1454 PYb Agar, 1454 Pycnoporus cinnabarinus, 1053 PYCS Medium. 1454 PYB Medium, 1455 PYEAAgar. 1455 PYEM Medium. 1455 Pyes Medium. 1456 PYEX Glucose Salt Medium, 1456 PYF Medium. 1456 PYGAgar, 1456 PYG Broth. 1457 PYG Medium, 1457, 1458 PYG Medium (B). 1458 PYG Medium (F.), 1458

2015

PYCJ Medium tor Spirillum. 1458,1459 PYG Medium with Volatile Fatty Acids, 1459 PYG Medium, Modified, 1458 PYG with 0.1% Twccnâ&#x201E;˘, 80.1459 Pygeye Agar, 1460 PYGHS Medium. 1460 PYGM Medium, 1460 PYGSAgar. 1461 PYG V Agar, 1461 PYGV Marine Medium. 1461 PYGV Medium. 1462,1463 Pyocin production, 1443 Pyocyanin, 1440 Pyocyanin pigment production, 1690 Pyocyanin production. 1445 Pyrazinamidase Agar, 1463 Pyrazinamidasc production, 1463. 1464 Pyra/inamide Medium, 1463 Pyrenochaetafallax. 431 Pyrenophora trilicirepentis, 1348 l*yrenula nitida. 951 Pyriculuna oryzae. 1438 Pyridine Medium, 1464 Pyridoxine assay. 1284, 1464 Pyridoxine Assay Medium. 1464 l*yridoxine Y Medium. 1464 l*yrobacuium aerophilum, 276 Pynbaeulum calidifontis Medium. 1464 l*yrobaculum islandicum. 1465 l*yn>baculum Medium. 1465 Pyrobaculum organolrophum, 1465 lymbaculum calidifontis. 1464 Pyrococcus endea\ori, 1465 1\>XKOCCUS endeavori Medium, lvS4.1465 Pyrococcus furiosus, 1466, 1467 h'rococcusfuriosus Medium, 1465 Pyrococcus Medium. 1466 fyrococcus woesei, 1466, 1467 lyrococcus/Staphylolhermtis Medium. 1466 fyrodictium abyssi, 1467 fyrodicrium abyssi Medium. 1467 fyrodiclium brockii, 1468 Pyrodiefiwn Medium. 1467 l*yrt)dictiwn occultum, 1468 I*ymlobus fumarii. 1468 Pyrolobus fumarii Medium, 1468 Avrwc/Hfl domesticum. 1410 tyrrolic acid utilizing bacteria. 1181 Pyrrol idone Agar, 1468 l*yruvate metabolization, 1469 Pyruvate Utilization Medium. 1468 l^-ruvic Acid Hgg Medium. 1469 PYS Agar, 1469 PYS Medium,, 1469 PYSF. Medium, 1469 Pythhan anandnan, 952 Pylhium aphanideitnatum, 1626 Pythium graminicola. 1626 Pythium insidiosum. 930 Pythium irregulare. 952 Pythium myriotylum, 1626

2016

Index

I'ylhitun ullimum. 1626 Pythium vexaus. 952 Pylhiumsylvalicum. 1626 Q Quaternary ammonium compounds, 948 Quinoline Medium. 1469 Quinolinc utilizing bacteria. 1182 Quinolinic acid. 1470 Quinolinic Acid Medium. 1470 R R Agar, 1470 R Agar for Phage l.ysates, 1470 R Agar with 3% Sodium Chloride, 1470 R Agar with 5% Sodium Chloride, 1471 R Agar with Catalase. 1470 R Broth lor Phage Lysatcs. 1471 R Medium, 1471 R2 Broth. 1472 R2A Agar. 1472 R2A Agar, Modified, 1473 R2A HiVeg Agar, 1473 R2YE Medium. 1525 R3 Medium, 1472, 1473 R3AAgar, 1473 R70-2 Agar. Modi lied with Fructose. 1474 R70-2 Agar, Modified with Glucose, 1474 R70-2 Broth. Modified with Fructose. 1475 R70-2 Broth, Modified with Glucose, 1475 R8 Medium, 1473 R8A1I Medium, 1477 RA. 1472 Rabbit Blood Agar, 1476 Rabbit Blood. Citrated, 5 Rabbit Blood, Dcfibrinatcd. 5 Rabbit Dung Agar, 1476 Rabbit Food Agar. 1476 Rabbit Heart Infusion Agar. 1476 Rabbit Faked Blood Agar, 1476 Rabbit Serum Bovine Serum Albumin Tween 80 Medium, 1477 Rabbit Scrum BSA Tween 80 Medium, 1477 Rabbit Scrum Medium. 1477 RAP! Medium, 1486 Reinforced AF Medium, 1477 Raffinosc fermentation. 1390 Rahnella atpialilis, 651 Rainbow Agar ()157. 1477 Rainbow Agar Salmonella, 1478 RajHans Medium, 1478. 1535 RajHans Medium, HiVeg, 1478 Raka-Ray Agar. 1478 Raka-Ray No. 3 Medium, 1479 Rabtonta spp., 1304, 1830 Ramalina americana. 951 Rambach Equivalent Agar. 844. 1535 Rambach* Agar. 1479 Rap Broth, Modified, 1481 Rapcr Achyla Medium No. 1. 1479 Raper Achyla Medium No. 2, 1479

Rapid Coliform HiCromc 1 " Broth, 843 Rapid Fnterococci HiCromeâ&#x201E;˘ Agar, 843 Rapid estimation, 1567 Rapid Fermentation Medium, 1480 Rapid MiColiform Agar, 1480 Rapid HiColiform Broth, 1480 Rapid HiColiform HiVeg Agar. 1480 Rapid HiColiform HiVeg Broth, 1480 Rapid HiFntcrococci Agar, 1481 RAPID'F.coli,2Agar, 1479 XKWWY Enterococcus Agar. 1479 RAPID'/- mono Medium, 1481 Rappaporl Broth. Modified. 1481 Rappapoil-Vassiliadis Enrichment Broth, 1481 Rappaport-Vassiliadis Medium Semisolid, Modified with Novobiocin, 1482 Rappaport-Vassiliadis R10 Broth. 1482 Rappaport-Vassiliadis Soy Peptone Broth, 1482 Rarohacierfaecitahidus, 1470, 1935 Ralhayibacter rathayi, 454 Ratoon stunting disease, 1548 Raulin Neutral of Dierckx. 1472 Raymond's Medium, 1482 Ra/i's Medium. 1482 RB-l/RB-9 Medium, 1483 RBA. 592 RCAgar, 1514 RCM Medium, Modified, 1483 R-CW Medium, 1483 RF-101 Medium,. 1484 Reactivation with Liquid Medium, 246. 1484 Reactivation with Tryptone Soya Broth, 1484 Reclinomonas americana. 1589 Recreational waters, 153, 1690 Rectal. 267.271 Rectal swabs, 267, 271 Reddy's Differential Agar. Modified. 1484 Reduced nitrogen, 327 Reduced Salt Solution Medium. 1484 Reduced Transport Thud. 1485 References, 9 Regai>-I .owe Charcoal Agar, 1485 Regan-Lowe Medium, 1485 Regan-Lowe Semisolid Transport Medium, 1485 Reinforced AF Medium. 1486 Reinforced Clostridial Agar, 1486 Reinforced Clostridial Agar with Tween1 M, 1486 Reinforced Clostridial HiVeg Agar. 1486 Reinforced Clostridial HiVeg Broth, 1486 Reinforced Clostridial Medium. 1487 Reinforced Clostridial Medium with Casamiiii) Acids, 1487 Reinforced Clostridial Medium with Glycerol, 1487 Reinforced Clostridial Medium with Sodium Lactate, 1487 Reinforced Clostridial Medium with Uric Acid. 1487

Reinforced Clostridial Medium, Modified. 1470,1487 Renibacterium KDM2 Medium. 1488 Renibaclerium salmoninanun. 893, 1488 Reniforma strues. 1001 Replicate plating technique, 1826 Reuters Sorbic Acid Agar Base, 1488 Rl-Medium. 1488 RFC Agar. 1522 RGCA Medium, 1488 Rhakdostyia species. 1589 Rhamnose fermentation, 1391 Rhamnose Salts Medium, 1489 Rhizamocba species. 1454 Rhizobaeter daucus, 599 Rhizobiaccac. 1490, 1491, 1930 Rhizohium Agar, 1489 Rhizohium Bill Defined Agar, 1489 Rhizohium Bill Defined Broth, 1490 Rhizohium fredii. 1489, 1936. 1947 Rhizohium galegae. 1936, 1947 Rhizohium huahiii, 1947 Rhizohium japonicum Agar, 1490 Rhizohium leguminosarum. 1936. 1947 Rhizohium loti, 1489, 1936, 1947 Rhizohium Medium 1. 1490 Rhizohium Medium 2, 1490 Rhizohium meliloti, 1489, 1936, 1947 Rhizohium phaseoli. 1947 Rhizohium radiohacter, 1176 Rhizohium species. 750,943, 1008, 1291, 1490, 1491 Rhizohium trifolii, 1947 Rhizohium Iropici, 1489, 1936 Rhizohium X Medium. 1491 Rhizocionia Isolation Medium, 1491 Rhizoctonia species, 1491 Rhizomouas Medium, 1491 Rhizomonas suherifaciens, 1470, 1491, 1492 Rhizomouas suherifaciens Medium, 1492 Rhizophyictis harderi. 746 Rhizophylciis rosea, 746 Rhizopogon colossus. 1199 Rhizopofion ellenae. 1199 Rhizopogon species, 1199 Rhizoptis species, 593. 594 Rhocococcus species, 743 Rhodopseudomonas palustris. 1926 Rhodopseudomonas species, 1503, 1504 Rhodohaca bogoriensis. 1524 Rhodohacler adraiticus, 1492 Rhodobacier adriaticus. 873 Rhodohacler adriaticus Medium, 1492 Rhodobacier capsuiaius, 490,1493,1507, 1508. 1926,1938 Rhodohacler changlensis, 1492 Rhodobacier changlensis Medium, 1492 Rhodobacier Medium, 1492 Rhodobacier species, 1926 Rhodohacler sphaeroides, 1477, 1493, 1507, 1509.1879,1926, 1933.1938 Rhodohacler sulfidophilusy?n\ 1507, 1509

Index

Rhotlobacter veldkampii. 1494. 1495 Rhodohacter wldkampii Medium, 1493, 1494 Rluxlobium gokarnense, 1495 Rhodobium gokurnum. 1495 Rhotlobium gokurnum Medium,, 1495, 1496, 1497, 1498 Rhodobhan Medium, 1499 Rhodobium orientis. 1499 Rhodohlastus acidophilus, 1501 Rhodohlastus Medium. 1499 Rhotloblastus species, 1499. 1511 Rhodocista centenaria, 342 Rhodococci species. 1326 Rhodococcus ausiralis, 1382 Rhothcoccus chlorophenolicus. 1489 Rhodococcui coprophilus, 1330 Rhodococcus equi. 453, 454, 751. 1274, 1330,1429 Rhodococcus erythropolis, 169.899, 1045. 1172,1330,1482, 1914 Rhodococcus fascians, 453.454, 1368 Rhotfococtvs globertdus. 1300 Rhodococcus Iulcus. 898, 899.1470, 1482 Rhodococcus marinonascens, 1019, 1471 Rhodococcus maris. 899. 1330. 1429.1470, 1482 Rhodococcui rhodnii, 1330 Rhixiococcus rhodochrous, 751, 898. 1182, 1185, 1309,1330 Rhodococcus roseus, 1182 Rhoilococcus ruber, 898, 1330 Rhodococats rubropertinclus. 1175 Rhodococcus species, 594, 751, 752, 827, 828.967. 968.980. 1173. 1179, 1184. 1259. 1364. 1416, 1429.1470, 1910. 1929,1943 Rhodococcus torques. 1419 Rhodocyclus gelatinosus, 1926 Rhodocyx-lus Medium. 1499 Rhodocyclus purpureas, 1500, 1501, 1509 Rhodocyx-lus pwpureus Medium. 1500.1501 Rluxlocyxlus tenuis, 1477, 1507, 1509 Rhotloferaxfermentans. 1258. 1455 Rluxiofenix ferrireducens, 197 Rhodomicrobium Medium, 1501 Rhodomicrobium species, 1879 Rhodomicrobium vannielii. 47. 1501. 1506. 1508. 1879, 1926 Rhodopila globiformis, 1502, 1503 Rhoilopila globiformis Medium. 1501, 1502 Rhodopseudomonas acidophila. 47. 51, 1504. 1506. 1508 Rhodopseudomonas blastica. 1503, 1507, 1509.1938 Rluxlopseudomonas blastica Medium, 1502 Rhodopseudomonas globiformis Medium. 1503 Rhodopseudomonas julia. 1504 Rluxlopseudomonas julia Medium, 1503 Rhodopseudomonas marina, 1019, 1507 Rhodopseudomonas Medium, 1504

Rhodopseudomonas palustris, 1504. 1507, 1509,1880, 1926. 1938 Rhodopseudomonas rosea. 1507 Rhodopseudomonas rutila, 1477 Rhodopseudomonas rutila Medium, 1504 Rhtxiopseudomonas species, 1504, 1938 Rhodopseudomonas sulfoviridis, 1505 Rhtxlopseudomonas sulfoviridis Medium, 1504 Rhotlopseudomonas vmdis. 1504, 1507, 1509,1933, 1934 Rhixlopsuedomonas species, 1926 Rhodospirillaceae, 1500, 1501, 1505. 1506 Rhodospirillaceac Knrichmcnt Medium. 1505,1506 Rhodospirillaceac Medium. 1506 Rhodospirillaceae Medium, Modified, 1507 Rhodospirillum centenum. 342 Rhodospirillum fulvum. 1507, 1508. 1509 Rhodospirillum Medium, 1507, 1508 Rhodospirillum Medium, Modified I, 1508 Rhodospirillum Medium, Modified II. 1508 Rhoiiospirillum Medium. Modified 111, 1509 Rhodospirillum Medium, Modified IV, 1509 Rhodospirillum molischianum. 1507. 1509, 1930 Rhodospirillum photometricum. 1477, 1507. 1509 Rhodospirillum rubrum. 1477. 1493. 1507. 1509, 1926, 1938 Rhodospirillum salexigens, 1507 Rhodospirillum salinarum. 799. 1510 Rhodospirillum salinarum Medium, 1509 Rhodospirillum sodomense. 609 Rhodospirillum species, 342, 1507, 1508 Rhodosporidium fxdudigenum. 1001 Rhixlosporidium toruloides, 1941, 1942 Rhodothermus marinus. 1219. 1748 Rh(xlotorula graminis. 1941, 1942 Rhodotonda matritensis. 750 Rluxiotorula mucilaginosa, 1001 Rhodovibrio sodomensis. 609 Rhtxlovulum imhoffii, 1201 Rhodovulum iodosum. 1511 Rhodovulum iodosum/Rhodovulum robiginosum Medium, 1510 Rhotlovulum kholense Medium., 1511 Rluxiovulum orientis, 1499 Rhodovulum robiginosum, 1511 Rhodovulum strictum, 1511 Rhoiiovulum strictum Medium, 1511 Rluxiovulum suljidophilum, 1512 Rhodovulum sulfidophilum Medium. 1512 Rhtxlovulum \nsakluun. 1512 Rhotlovulum visakhum Medium, 1512 Rhynchomonas nasuta, 1015, 1589 Rhynchopus species. 644 Rhynchosporium secalis, 206 Rhyzopltydium species, 980 Riboflavin assay, 1512 Riboflavin Assay Medium, 1512 Riboflavin Medium, 1512

2017

Ribose Production Medium, 1513 Rice I'xlmcl Agar, 1513 Rice Grain Medium. 1513 Rice Infusion Oxgall Tween 80 Agar, 1514 Rich Medium. 1513 Riemerella species, 1830 Rifampicin Luria Agar. 1513 Rikenella microfusus, 631, 718 Rila Marine Medium. 1514 Rimler-Shotts Medium. 1520 Rimlcr-Sholls Medium. 1514 RIOT Agar, 1514 Rippey-Cabelli Agar, 1514 Rippcy-Cabelli 1 liVcg Agar Base wiUi lvthanol and Ampicillin, 1514 RM Medium, 1514 Rogosa Agar, 1515 Rogosa Broth, Modified, 1515 Rogosa Selective Inctobacillus Agar, 1516 Rogosa Selective Ixtctobacillus Broth, 1516 Rogosa SI. Agar, 1516 Rogosa SL Broth. 1516 Rogosa SI. HiVeg Agar. 1516 Rogosa SL Hi Vcg Broth. 1516 Rollandina capitafa. 706 Rollandina hyalinospora, 706 Rolled Oats Mineral Medium. 1517 Root canals. 1593, 1843 Root nodules, 703. 704. 1490 Roots, 165 Rosculus species, 707 Rose Bengal Chloramphenicol Agar, 1517 Rose Bengal Chloramphenicol HiVeg Agar. 1517 Roseateles depolymerans. 1384 Roseburia cecictda. 1064 Roseicyclus Medium. 1517 Roseicyclus species, 1518 Roseiflexus castenholzii, 356 Roseinatronobacter Agar. 1518 Roseinatronobacler Medium, 1518 Roseinatronobacter thitxj.xidans, 1518 Roseivivax halotolerans, 1420 Roseobacter denitrijicans, 1420 Roseohacter species, 1009 Roseococcus thiosulfatophilus. 652 Roseospira gtxrnsis, 1499 Roseospira navarrensis, 1060 Roseospira visakhapatnamensis, 1498 Roseospira species. 1059 Roseovarius nubinhibens, 1953 Roseovarius tolerans, 635 Roufs Medium., 1518 RP Medium. 1519 R1T' Supplement, 5 RPMI, 1640 Medium with l.-Glutamine, 1520 RS HiVeg Medium Base with Novobiocin, 1520 RS Medium. 1514 RSS Medium. 1484 R-Top Agar, 1471

20 IS

Index

Rubiteleu Medium.. 1520 Rubritalea Medium. 1520 Rubritalea species, 1521 Ruhritepitla Jlocculans, 1473 Rubrivtvax gelatinosus. 1507,1509, 1938 Rubrobacier xyianophilus, 1747 Ruegeria atlanlica, 1009 Rumen Bacteria Medium. 1521 Rumen Fluid Ccllobiose Agar. 1522 Rumen treponemes, 1522 Rumens, 275,860 Ruminobacter amylophilus. 1419, 1522 Ruminobacter amylophihts Medium. 1522 Kuminococcusalbta, 1419,1489, 1523 Riiminococcus albus Medium, 1522 Riiminococcus bromii. 1419 Ruminococcusflavifaciens, 1419 Riiminococcus pasteurii, 1523, 1524 Riiminococcus pasteurii Medium. 1523 Riiminococcus species, 367. 369 Riiminococcus torques, 370 Runella slithyformis, 116,1237. 1524 Rimella slithyformis Medium. 1524 Russell Double-Sugar Agar, 605, 1524 RV Enrichment Broth, 1481 RV5 Medium. 1524 RVS Broth, 1482 Ryan's Aeromonas Medium, 61 S S Broth. 1525 S Medium, 1525 S Salts, 1526 S. aureus ID. 1525 S.F.P. HiVeg Agar Base with I-gg Yolk and Antibiotics, 1569 S6 Medium lor 'lliiohacilli, 1526 S8 Medium for Ihiobacilli, 1526 SAAgar, 1526 SA Agar. Modified. 1526 SA HiVeg Agar Base with Ampicillin, 1526 SablXx, 2%, 1530 Sabl)cx.4%, 1530 SABIII HiVeg Agar Base with Blood and Chloromycetin, 1528 SABIII HiVcg Agar Base with Chloramphenicol, 1528 SABIII HiVeg Agar Base with Chloromycetin, 1528 SABIII* Agar, 1527 SABIII* Agar. Modified. 1527 SABIII8-Blood Agar, 1527 Saboraud IXwtrose Agar. 1530 Sabouraud Agar, 1528 Sabouraud Agar with CCG and, 3% Sodium Chloride. 1528 Sabouraud Agar with CCG and. 5% Sodium Chloride, 1529 Sabouraud Agar. Diluted 1/10, 1529 Sabouraud Agar, Diluted 1/10 with Salt, 1529 Sabouraud Agar. Modified, 1529

Sabouraud Chloramphenicol HiVeg Agar. 1530 Sabouraud Cycloheximidc Chloramphenicol HiVeg Agar, 1530 Sabouraud Dextrose Agar, 1530 Sabouraud Dextrose Agar pH 5.6, 1530 Sabouraud Glucose Agar, 1530 Salxnuaud Glucose Agar with Chloramphenicol and Cycloheximidc, 1531 Sabouraud Glucose Agar with Olive Oil, 1531 Sabouraud Glucose Agar. hmmons. 1531 Sabouraud Glucose Agar, HiVeg, 1531 Sabouraud Glucose and Brain Heart Infusion Agar, 1527 Sabouraud Glucose Broth. 1531 Sabouraud Glucose HiVeg Agar Base, Modified. 586 Salxmraud Glucose HiVeg Agar Base, Modified with Cycloheximidc and Chloramphenicol, 1532 Sabouraud Glucose HiVeg Broth, 1532 Sabouraud Glucose Maltose 1 liVeg Agar. 1532 Sabouraud Liquid Broth, Modified. 132 Salxmraud Liquid HiVeg Medium, 1532 Sabouraud Maltose Agar, 1532 Sabouraud Maltose Broth, 1532 Sabouraud Maltose HiVeg Broth, 1532 Salxmraud Medium, Hmmons Modification, 1532 Sabouraud Medium, Fluid, 1533 Sabouraud Medium. Fluid. HiVcg. 1533 Sabouraud's Agar, Modified, 639 Saccamoeba Umax. 707 Saccharibacterfloricota, 1950 Saccftarobaclerium acuminatum. 1182 Saccharobacteriunt ovale. 1171 Saccharococcus Agar, 1533 Sacchurococcits thermophilus, 1533, 1838, 1852 Saecharolytic ClosU idia Medium, 1533 Saccharolytic Clostridium. 1534 Saccharomonospora caesia. 469. 481. 1318 Saccharomonospora glauca, 210, 753 Saccliaromonosf)ora halophila. 1637 Saccharomonospora intematus, 886 Saccharomonospora saliphila, 11 Saccharomonospora viridis, 469,481, 767, 1312.1330. 1825 Saccharomyces bayantts, 750 Saccharomyces cerevisiae, 15.469,470,750, 753, 843, 988, 1001, 1055, 1187,1188, 1189,1468.1513. 1534, 1544.1545, 1546, 1547, 1548, 1558. 1559,1577, 1672, 1909,1924, 1925, 1939, 1940, 1941,1942.1946.1949. 1950 Saccharomyces cerevisiae ATCC 36375, 1937 Saccharomyces dottglasii, 750 Saccharomyres exiguus, 1000 Saccharomyces Medium. 1534

Saccharomyces paradoxus, 750 Saccharomyces pasteurianus NRRI. Y-139, 1645 Saccharomyces rouxii. 1413 Saccharomyces rouxii Medium, 1534 Saccharomyces servazzii, 589 Saccharomyces species, 1909, 1941, 1942, 1949 Saccharomyces itvarum, 876, 1464 Suix.haromyx.odes ludwigii. 1001 Saccharomycopsisfibuligera, 1001 Saccharopolyspora hirsuta, 469, 767, 886, 1330 Saccharopolyspora hordei. 1363 Saccharopolyspora rectivirgula, 84.214. 469. 1330, 1363.1588. 1645 Saccharothrix aerocolonigenes, 1318. 1330. 1939 Saccharothrix coendeofusca. 767, 1323 Saccharothrix coendeoviolacea. 639 Saccharothrix longispora, 767, 1323 Saccharothrix mutabilis, 1939 Saccharothrix mutabilis subsp. mutabilis. 1318 Saccharothrix species, 768 Sakazakii DHL Agar, 1534 Saksenaea vasiformis, 809 Salad dressings. 923 Salegentibacter salegens, 1009 Salicin fermentation. 1391 Salicylic acid utilization. 1182 Saline Czapck Agar. 1534 Salinibacter ruber, 1534, 1535 Salinihacter ruber Agar, 1534 Salinibacter ruber Medium. 1535 Salinicoccus alkaliphiius. 853. 854 Salinicoccus hispanicus, 851 Salinicoccus roseus, 851, 1346, 1617 Salinicola socitts. 1580 Salinisphaera shabanensis. 1553 Saliniwbrio costicola subsp. vallismortis. 1535 Salinivibrio costicola subspecies vallismortis Medium, 1535 Salinococcus hispanicus. 1155 Salinococcus roseus. 1155 Saliva. 200.892. 1515. 1516. 1517.1583 Salmonella Agar, 843 Salmonella Agar. HiCrome IM , 844 Salmonella Agar. Modified, 843 Salmonella hongori, 215 Salmonella choleraesuis, 130,131,644,762, 914.934, 1308.1310.1313. 1315.1536 Salmonella choleraesuis subsp. hongori, 215 Salmonella Cliromogen Agar, 1535 Salmonella Chromogen Agar, HiCrome1*1, 844 Salmonella Chromogenic Agar. 1535 Salmonella Differential Agar. 1535 Salmonella Differential Agar. Modified. HiVe, 1536

Index

Salmonella Differential 1 liVeg Agar. Modilied, 1536 Salmonella enteritidis, 889 Salmonella HiVeg Agar, ONOZ, 1536 Salmonella Medium. 1536 Salmonella paratyphi, 1559 Salmonella paratyphi A, 889 Salmonella Rapid Test I elective Medium, 1536 Salmonella Rapid Test Elective Medium, 2X, 1536 Salmonella Shigella Agar, HiVeg, 1625 Salmonella Shigella Agar. Modified. 1537 Salmonella Shigella Agar, 1536 Salmonella Shigella Deoxyeholate Agar, 1625 Salmonella species. 201. 212. 224, 241, 242, 260. 261, 262. 263, 282. 283, 337,377, 386,485, 486,493,494,495,496,613, 692.693, 740. 755. 770. 817.818.820. 831, 841, 843, 844, 889,928, 943,952, 975,976,977,978,991,992,1005,1008, 1030.1032.1037. 1196.1217.1227. 1251, 1301, 1327,1339,1386,1392, 1409. 1410.1478.1479,1482.1535. 1536. 1537, 1541, 1559, 1560, 1562. 1564.1625,1695. 1699,1700, 1701, 1822.1835,1837. 1831.1849. 1908. 1909. 1925 Salmonella typhi, 212, 223,224, 260. 262. 263.1030,1247.1339.1559.1699.1700. 1701,1908,1909 Salmonella typhimurium, 1704

Salmonellac, 1247, 1699. 1925 Salpingoeca ttreeolata, 1015 Salt Agar, 1537 Salt Broth. Modified. 1537 Salt Colistin Broth, 1537 Salt Malt Agar, 1537

Salt Meat Broth, 1537 Salt Medium, 1538 Salt Nutrient Agar, 1538 Salt Polymyxin Broth. 1538 Salt Polymyxin HiVeg Broth Base with Polymyxin, 1538 Salt tolerance, 968, 1537, 1538,1539,1584 Salt Tolerance Medium, 1538 Salt Tolerance Medium, (iilardi, 1539 Salt Tolerance Medium, latum. 1539 Salted foods, 800, 802 Salted vegetables. 960 Sanfrancisco Medium, 1539 Sanitary, 261.263,991,992.1031.1032. 1826 Sanitizalion. 1156. 1157 SAP. 1 Agar, 1539 SAP. 2 Agar. 1539 Saprophytic, 1261 Saprospira albida, 688 Saprospira grandis, 477. 687, 1015, 1054, 1540 Saprospira grandis Medium. 1539

Saprospira species. 135, 340, 349. 472,475, 610, 709, 718, 856, 1237, 1238, 1350, 1539.1574. 1594. 1597. 1626.1629. 1640, 1667, 1669 Saprospira species, 856 Saprospira thennalts, 688 Sarcina lutea NRRL B-1018.1645 Sareina maxima, 1540 Sarcina maxima Medium, 1540 Sarcina Medium, 1540 Sarcina species, 1158 Sarcina wntriculi, 1540 Sarcina ventriculi Growth Medium, 1540 Sarcodon aspratu. 1199 Sareogyne simplex, 951 Sauton's Fluid Medium Base. 1540 Sauton's Medium, 1540 SB/SW Medium. 1541 SBG Lnrichment Broth, 1540 SBCi Sulfa Enrichment, 1541 SCAgar, 1542,1543 SC Agar without Hislidine. 1544 SC Agar without Leucine and Tryptophan, 1544 SC Agar without Uracil. 1545 SC Broth. 1544.1545. 1546 SC Broth without Hislidine. 1546 SC Broth without Leucine and Tryptophan, 1547 SC Broth without Uracil, 1547 SC Medium. 1548 Scenedesmus annatus, 70 Scenedesmus communis. 70 Scenedesmus ahliquus, 70 SCGY1-M Medium. 1548 Schaedler Agar, 1549 Schacdler Agar with Vitamin K( and Sheep Blood, 1549 Schaedler Anaerobic Agar. 1549 Schaedler Anaerobic Broth. 1549 Schaedler Broth, 1549 Schaedler CNA Agar with Vitamin K( and Sheep Blood, 1550 Schaedler HiVeg Agar with Blood, 1550

Schaedler HiVeg Broth, 1550 Schaedler KV Agar with Vitamin K( and Sheep Blood, 1550 Schineria larvae, 1440 Schizochylrium aggregation. 287 Schizophyllum commune. 775 Schizophyllum Medium, 1551 Schizophyllum species, 1551 Schizosaccharomyces japonicus, 1928 Schizosaccharomyces Malate Medium, 1551 Schizosaccharomyces pombe, 750, 1551, 1928,1941, 1942.1949 Schlcifer-Kramer Agar, 1551 Sehmitthenner's Agar, 1626 Schneider's Drosophila Medium, 1551 Schuberts Arginine Broth, 1551 Schuster's Axenic Naegleria Medium, 1552 Schwanniomyces occidentalis. 1941, 1942

2019

Schwartz Differential HiVeg Medium, 1552 Scleroderma alhidum, 1199 Sclerophoma pityophila. 1003 Sclerolinia sphaerosperma, 809 Sclerotium cepivomm. 1410 Scopulariopsis ftmicola, 952 SCY Medium, 1552 Scylonema species, 213, 214 SCZA, 1534 SI) Medium, 1564 SIX) Medium.. 1553 SDS HiVeg Agar with Polymyxin B. 1553 Si; HiVeg Broth. 1642 Seafood. 1553,1885 Scawatcr. 11 Scawatcr. 802 Medium, 1556 Seawater, 802 Medium. Half-strength. 1556 Scawatcr Agar. 1553. 1554 Seawater Agar Modified, 1555 Scawatcr Agar with 1% Scrum, 1555 Seawater Agar with Petal Calf Serum, 1554 Seawater Agar with Horse Blood. 1554 Seawater Agar Medium, 1555 Scawatcr Basal Medium. 1555 Seawater Complete Medium, 1555

Seawater Lcmco Agar. 1555 Seawater I.emco Broth, 1555

Seawater Medium, 1556 Seawater Nitrvsomonas Medium. 1556 Seawater Nutrient Agar, 1557 Scawatcr Spirillum Medium. 1557 Seawater with Serum, 1557 Seawater Yeast Lxtract Agar, 1557 Seawater Yeast Lxtract Bioth, Modified, 1557 Seawater Yeast Lxtract Peptone Medium, 1558 Seawater Yeast Peptone Agar, 1558 Seawater Yeast Peptone Medium, 1558 Seawater YPG, 1558 Sediment, 439 Sediment samples, 156. 1327 Sedimentihacter saalensis, 1954 Sedimenticola selenatireducens, 1561 Seed Agar, 121.130 Seed HiVeg Agar. 126 Selection Agar I, 1558 Selection Agar II. 1558 Selective 71111 Agar. 1566 Selective Components. 6 Sclenatc Reducer Medium. 1560 Selenite Brilliant Green Lnrichment Broth, 1540

Selenite Broth, 1559 Selenite Brotfi Base, Mannitol, 1559 Selenite Bioth, Lactose, 1559 Selenite Cystine Broth, 692. 1559 Selenite Cystine HiVeg Broth, 692 Selenite F Broth. 1559 Selenite F Enrichment Medium. 1559 Selenite Mannitol Broth. 1007 Selemle-F Broth with Dulcitol, 613

2020

Index

Selenomonas actdaininophiia. 1561 Selenomonas acidaminophih Medium, 1561 Selenomonas acidaminovorans, 1906 Selenomonas laclici/ex, 12.16, 1456 Selenomonas noxia. 368 Selenomonas rnminantinm, 339, 616, 718, 1419.1561 Selenomonas rnminantinm Medium, 1561 Selenomonas Selective Medium. 1561 Selenomonas species, 371, 617, 631, 1561 Selenomonas sputigena, 366. 718. 1459 Selenomonas suis, 616 Sellers Agar, 1561 Sellers Differential Agar, 1561 Sellers Differential HiVcg Agar. 1561 Semiolid Medium. Modified. 1562 Semiselective Medium for Legionella pneumophila, 234 Semisolid Brucella Broth, 1562 Semisolid BSA Twccn 80 Medium. 240 Semisolid IMRV HiVeg Medium Base with Novobiocin. 1564 Semisolid Medium for Motility. 1563 Semisolid Medium. Modified with Cysteine and Neutral Red. 1562 Semisolid Medium. Modified with NaCl and Neutral Red. 1563 Semisolid Medium, Modified with Nitrate and without Neutral Red. 1563 Semisolid Pectin Agar, 1563 Semisolid RV HiVeg Medium Base with Novobiocin, 1564 Sensitest Agar. 1564 Sensitivity assays, 1564 Sensitivity Test HiVeg Medium with Blood Serum, 1564 Septoria apiicola, 335 Septoria nodorum. 1903 Serological identification, 1800 Serological typing, 1799. 1800 Serpens flexibilis, 916 Serpula hyodysenteriae, 659 Serpula innocens, 1831, 1832 Serpidina hyodysenteriae. 253 Serpidina pilosicoli, 253 Serratia Differential Medium, 1564 Serratia Ud-MUr, 1565 Serratia liquefaciens. 1565 Serratia marcescens, 91,207, 250. 603. 604, 882. 1382. 1565,1829. 1833,1928 Serratia Medium, 1565 Serratia ruhidaea, 1565 Serratia species. 96. 218.465.609, 1565. 1702 Serum Glucose Agar, 1565 Serum Glucose Agar, I'airell Modified, 1565 Serum Potato Infusion Agar. 1566 Serum Tellurite Agar, 1566 Setosphaeria rostrata. 1877 Seudoalleroinonas espejiana, 1558 Seven III I Agar. 1566 Seven-1 lour PC Agar. 1567

Seven-Hour fecal Colilbrm Agar, 1567 Sewage, 141, 158, 162, 163. 261, 290,291, 649. 650, 655. 1026. 1069, 1242. 1247. 1303, 1314,1648, 1699, 1938 Sf Broth. 1567 Sf HiVeg Broth, 1567 Sf 1 Medium, 1567 SIP Agar, 1568 5 0 Agar. 1569 Shahidi-Perguson Perfringens Agar, 1568 Shanorella spirotricha, 706 Shapton I ItVeg Medium. 1569 Shapton Medium, 1570 Sheep Blood Agar. 1570 Sheep Blood, Citrated, 5 Sheep Blow!. Dclibrinatcd. 5 Shepard's Differential Agar, 13 Shepard's M10 Medium, 1630 Shewanella alga, 674 Shewanella algae, 1558 Shewanella atlantica, 1215 Shewanella hanedai, 971, 1395 Shewanella putrefaciens, 116, 670 Shigella Broth, 1570 Shigella dysenleriae, 1310 Shigella Jlexneri, 1310 Shigella 1 liVeg Broth Base with Novobiocin, 1570 Shigella species. 22,201,445.485.486.493. 494. 495,496. 755,817, 818. 992, 1248. 1308. 1409,1410, 1537. 1570.1571, 1625,1832.1919, 1920. 1925 Shiitake Agar, 1571 51 Agar, 1571 Siderophore Mineral Medium, 1571 Sierra Medium, 1571 Silicihacler pomeroyi, 1953 SIM HiVcg Medium. 1571 SIM Medium, 1572 SIM Motility Medium. 1572 Simmons' Citrate Agar, 1572 Simmons' Citrate Agar, Modified, 22 Simonsiella Agar, 1572 Simonsiella Broth, 1573 Simonsiella erassa. 1309 Simonsiella muelleri, 1573 Simonsiella species. 279, 1826. 1827 Simonsiella steedae, 1573 Simulated Crape Juice Medium, 1573 Singh's Medium, Modified, 1573

Skim Milk Horn Meal Mineral Agar, 1575 Skin, 192,193,496,497,657 Skirrow Brucella Medium, 1576 Skirrow's Campylobacter Agar, 299 SL Medium. 1576 SI .A Trace flements, 5 Slad Medium, 1577 Slanetx and Bartley HiVeg Medium, 1577 Slanctz and Bartley Medium, 1577 Slanetz and Bartley, HiVeg, 1577 Sludge. 776 Sludge Medium for Methanobacteria. 1577 Sludge Medium for Methanobacteria, pi 17.9, 1578 SM Basal Salts Medium, 1578 SM Medium. 1579 SM Selective Medium, 1579 SMA1. 1537 SMB Medium, 1579 SMC Medium. 1580 SMC, Modified. 1580 SMK Agar. 1581 SME Medium Modified for IS7, 1582 SMK Medium, 1581 SMI- Medium, Modified, 1581 Smibert's Semisolid Brucella Medium. 1582 Smithella Medium. 1582 Smithella species, 1583 Smittiu/n culicis. 411, 1146. 1839 Smittium culisetae, 477, 1146, 1839 Siniltium mucronatum. 248 Smittium simulii, 411, 1146, 1839 Smittium species, 477. 1146, 1839 Smut fungi, 1417 SNA. 1557 Snyder Agar, 1583 Snyder Test Agar. 200.1583 Snyder Test HiVeg Agar, 200.1583 Soap Agar, 1583 SOB Medium, 807 Sodalis glossinidius. 1584 Sodalts glossinidius Medium,, 1584 Sodium Acetate Agar, 22 Sodium Acetate Medium I. 1584 Sodium Biselenite Medium, 1559 Sodium Caseinate Agar, 1584 Sodium Chloride Broth. 6.5%. 1584 Sodium Chloride SUC Medium 900, 1584 SiRlium Chloride Sucrose Medium 900, 1584 Sodium Chloride Sucrose Medium 900 with

Single-Layer Agar, 1573

Penicillin G, 1585 Sodium Dcsoxycholatc. 7 Sodium Dodecyl Sulfate Polymyxin Sucrose Agar. 1585 Sodium Dodecyl Sulfate Polymyxin Sucrose HiVeg Agar, 1553 Sodium Hippurate Broth. 1585 Sodium Hydrogen Selenite Medium, 1559 Sodium Lactate Agar. 1585 Sodium Lactate Agar, Modified. 1585

Singulosphaem Medium,, 1574 Singulosphaera species. 1574 Sinorhizobium xinjiangensis, 1491 Six B Agar. 1574 SJ Agar, 1574 SKAgar, 1551 Skim Milk Acetate Medium, 1574 Skim Milk Agar. 1575 Skim Milk Agar, Half Strength, 1575 Skim Milk Glucose Agar, 1575 Skim Milk HiVeg Agar, 1575

Sodium Taurocholate, 7 Soil Agar Gelatin Overlay, 1586

Index

Soft-rot, 702 Soil, 58,147, 151,170, 192, 193, 210, 254, 355.379,418, 439.657. 709. 774.1040. 1041, 1900 Soil bacteria, 1318 Soil lvxtract, 5,1586 Soil Extract Agar, 1586

Soil Extract Glucose Yeast Extract Agar, 1587 Soil lvxtract Glycerol Medium. 1587 Soil Kxtract Medium, 1587 Soil lvxtract Peptone Beef lvxtract Medium. 1587 Soil lvxtract Potato lvxtract Medium. 1587 Soil Extract Salts Medium, 1588 Soil samples. 1636 Soil Seawater Medium lor Algae, 1588 Soils. 749 Soluuon sterility, 1773 Soimebom's Paramecium Medium, 1589 Sorangium Medium, 1589 Sorbitol Agar. 1589 Sorbitol fermentation, 1391 Sorbitol HiVeg Agar, 994, 1589 Sorbitol MiVeg Agar with Blood, 1589 Sorbitol Iron IliVeg Agar, 1590 Sorbitol MacConkey Agar, 1590 Sorbitol MacConkey HiVcg Agar. 1589 Sorbitol Medium, 1590 Sorbitol Medium, 5%, 1591 Sorbose, 239 Sordana hre\icoilis, 472, 486 Sorogena Medium, 1591 SOT Medium, 1591 Sour Dough Medium. 1591 Sources of Media, 8 Soy peptone. 3 Soy Peptone BroUi, 1592 Soybean Agar, 1592 Soybean Casein Digest Agar, 1825 Soybean Casein Digest Agar. IliVeg. 1592 Soybean Casein Digest Broth, USP, 1830 Soybean Casein Digest Medium, HiVeg, 1592 Soybean lvxtract M-l, 1592 Soybean 1 liVcg Broth Base with Novobiocin, 1592 Soybean HiVeg Medium. 1593. 1843 Soybean 1 liVcg Medium with 0.1% Agar. 1593. 1843 Soybean 1 liVeg Medium with 0.1% Agar with Glucose, 1593 Soybean 1 liVeg Medium with Yeast lvxtract

and Ferric Pyrophosphate, 1593 Soytonc. 2 SP, 2 Agar. 1594 SP. 6 Agar, 1597 SPAgar, 1593 SP Medium, 1594 Sl*2 Agar, 1594 SIM Medium. 1594 SP4 Medium with Glucose. 1596

SP4 Medium,, 1595

SP4-/. Medium with Glucose and DNA, 1620 SP5 Broth, 1597 SPB, 1538 Special Infusion Agar. HiVeg with Blood, 1597 Special Infusion Broth. HiVeg with Blood. 1598 Special peptone. 3 Specimen Preservative Medium, 1598 Spfiaericus Spore Medium. 1598 Sphaerobaeter tkermomethanica, 1880 Sphaerobacter thermophilic, 1332 Sphaerostilbe repens, 952 Sphaerotiius Agar, 1598 Sphaerotiius and Leptothrix Ivnrichmcnt Medium, 1599 Sphaerotiius CGYA Medium. 1598 Sphaerotiius IX'fincd Medium, 1598 Sphaerotiius discophoms, 1005.1599 Sphaerotiius discophoms Medium, 1599 Sphaerotiius Isolation Medium. 1599 Sphaerotiius Leptothrix Medium. 1599 Sphaerotiius Medium. 1599 Sphaerotiius nutans, 926,946, 1005, 1598, 1599.1600. 1640. 1938 Sphaerotiius nutans Ivnrichmcnt Medium. 1600 Sphaerotiius natans Isolation Agar. 1600 Sphaerotiius natans Medium, 1600 Sphaerotiius species. 323. 1598.1599 Sphaerottlus-Leptothrix Agar, 1599 Sphingohacterium Medium. 1600 Sphingobuclenum multivorum, 1304, 1600 Sphingohacterium spirifiwntm. 1304.1600 Sphingohium chlorophenolicum, 826,829, 1180.1484 Sphingohium herbicidovorans, 1447 Sphingomonas asaccharolylica, 869 Sphingomonas aurantiaca, 1939 Sphingomonas chlorophenolica, 829, 1180, 1484 Sphingomonas mali, 869 Sphingomonas paucimobilis, 1404 Sphingomonas pruni, 869. 1447 Sphingomonas rosa. 869 Sphingomonas sanguinis, 215 Sphingomonas sanguis, 215 Sphingomonas species, 1051, 1056. 1173, 1180. 1401. 1939 Spinal fluid. 585 Spingohacterium mizutae, 1600 Spiriilospora aibida. 480, 481. 1411 Spinllospora rubra, 1323 Spiriilospora species, 1939 Spirillum gracile, 1601 Spirillum gracile Agar. 1600 Spirillum gracile Broth, 1601 Spirillum gracile Medium, 1601 Spirillum leptoferum, 1601 Spirillum lipoferum Medium. 1601 Spirillum Medium, 1602

2021

Spirillum Nitrogen-Fixing Medium, 1602 Spirillum pleoinorphum, 1459 Spirillum species, 1170,1366, 1557,1602 Spirillum vohaans, 1230.1366, 1602 Spirillum wlutans Defined Medium. 1602 Spirit Blue Agar. 1602 Spirit Blue HiVeg Agar, 1602 Spirochaeta africana, 78 Spirochaeta alkalica, 78 Spirochaeta americana, 1603 Spirochaeta americana Medium,, 1603 Spirochaeta asiatica. 78 Spirochaeta aumntia, 811.1230,1231,1362. 1604 Spirochaeta aurantia Agar, 1603 Spirochaeta aurantio Growth Medium, 1604 Spirochaeta aurantia Isolation Medium. 1604 Spirochaeta bajacaliforniensis, 1019. 1020 Spirochaeta caldaria, 1604. 1852 Spirochaeta caldaria Medium, 1604 Spirochaeta halophila. 876, 881. 1605 Spirochaeta haiophila Medium. 1604 Spirochaeta isovalerica, 1016 Spirochaeta isovalerica Medium. 1605 Spirochaeta liloralis, 1605, 1606, 1608 Spirochaeta liloralis Medium, 1605, 1606 Spirochaeta smaragdinae, 1606 Spirochaeta smaragdinae Medium. 1606 Spirochaeta species, 277, 1474 Spirochaeta slenoslrepta, 769, 1607 Spirochaeta slenoslrepta Medium, 1606, 1607.1611 Spirochaeta thermophila. 314. 1609. 1733 Spirochaeta zuelzerae. 1607 Spirochaeta zuelzerae Medium, 1607 Spirochete Ivnrichmcnt Medium, 1607 Spirochete Medium. 1607. 1608 Spirochete Thermophilc Medium. 1608 Spirochetes. 894, 1607. 1608.1609 Spirochetes aurantia. 1604 Spirolatc Broth, 1609 Spirolate HiVeg Broth. OMA'l A with Scram 1609 Spiromyces minutus, 1476 Spironucleus vortens, 1861 Spiroplasma Agar MID. 1609 Spiroplasma alleghenense, 1620, 1621 Spiroplasma apis, 1261, 1265, 1421, 1423, 1472 Spiroplasma Broth MID. 1610 Spiroplasma chinense, 1620. 1621 Spiroplasma citri, 279. 1261. 1265. 1472. 1580, 1581,1611 Spiroplasma clarkii. 1620, 1621 Spiroplasma cxjmiscae, 1620. 1621 Spiroplasma culicicola, 1620, 1621 Spiroplasma Jloricola, 1402, 1472, 1611 Spiroplasma kunkelii, 290, 1402, 1610 Spiroplasma Medium, 1610, 1611

2()22

Index

Spiroplasma Medium with 25 mg/E llienol Red, 1611 Spiroplasma melliferum. 1040. 1261. 1265, 1402, 1472 Spiroplasma species. 289.980,1061, 1402. 1573,1610, 1620, 1621 Spirosoma linguale. 116. 117, 1158, 1838 Spiralina maxima. 1591 Spirulina platensis, 1591 Spiralina species. 213,214 SPMA, 1185 Sporanaerobacter acetigenes DSM 13106. 826 Spore production, 1309, 1316 Spore Strip Broth, 1611 Spore strips. 1611 Spore suspensions, 66 Spores. 439 Sporichthya polymorpha. 481. 767, 1321. 1939 Sporobacter Medium. 1611 Sporobacter termitidis, 1612, 1696 Sporobacteriam olearium, 823 Sporohohimves roseus, 1001 Sporocytopiutga Medium. 1611. 1612

Sporvcytophaga mywepcooides, 341, 1184, 1612 Sporohahbacter lortelii, 801, 802, 1612, 1613 Sporohahbacter lortelii Agar, 1612 Sporohahbacter lortelii Broth, 1613 Sporohahbacter marismortui. 790 Sporolactobacillus inuiinus, 453.768.920. 1233.1234 Sporolactobacillus species, 100, 749, 888, 1234 Sporomusa acidovorans, 1615 Sporomasa Medium. 1613, 1614 Sporomusa Medium, Modified, 1614, 1615 Sporomusa ovata. 1614 Sporomusa silvacetica. 1616 Sporomusa silvacetica Medium, 1616 Sporomusa species, 1614 Sporomusa sphaeroides, 1614 Sporomusa termitida. 1616 Sp<}rosarcina halophila, 798, 1019, 1617 Sporosarcina hahphila Agar. 1616 Sporosarcina pasteurii, 1313 Sporosarcina ureae. 182, 1617 Sporosarcina ureae Medium, 1617 Sporothrix schenckii, 251, 1532.1952 Sporotomaculum hydroxybenzoicum, 280 Sporotomaculwn syntrophicum. 669 Sporulating Agar, 66 Sponilating bacteria. 1618 Sporulation. 59, 60. 1214. 1618, 1901 Sporulation Agar, 1617 Sporulation Broth, 1618 Sporulation Medium, Modified. 613 Spray's Fomentation Medium, 1618 Spa-ad plate. 1472. 1473 SPS Agar. 1618

SI'S HiVeg Agar, 1618 SPS HiVeg Agar, Modified, 1619 SPY!: Medium 1619 SRB-Psychiophile Medium, 1621, 1622, 1623,1624 SS Agar, 1536 SS Agar. IliVcg. 1625 SS Agar, Modi lied, 1537 SS Dcoxycholatc Agar. 1625 SS Medium, 1561 SSDC. 1625 SSI. Agar. 1625 SSM Agar. 1626 ST Agar, 1644 ST Holding Medium. 1626 StA, 1626 STAA Agar Base, 1627 STAA Selective Supplement, 7 Stab Agar. 1627 Slachyamoeba species. 1589 Staib Agar. 223 Staley's Maintenance Agar, 1627 Staley's Maintenance Broth, 1627 Stalcya guttiformis. 635 Stan 4 Agar. 1628 Stan 5 Agar, 1628 Stan 5 Mineral Medium, 1629 Stan 6 Agar, 1629 Standard Agar with Methanol and Yeast Extract, 1629 Standard Fluid Medium, 10B, 1630 Standard I Medium. 1630 Standard I with Malt Agar, 1630 Standard II Nutrient Agar, 1632 Standard Infusion Agar, HiVeg, 1039 Standard Methods Agar. 1631 Standard Methods Agar wim Lecithin and Polysorbate, 80, 1631 Standard Methods Agar, HiVeg. 1403.1631 Standard Methods Broth, 1631 Standard Methods Casemate Agar, 1631 Standard Methods Caseinate HiVeg Agar, 1631 Standard Methods HiVeg Agar with Tween 80 and I,ccithin. 1632 Standard Nutrient HiVeg Agar, 1632 Standard Nutrient HiVeg Broth, 1632 Standard Plate Count procedure. 1838.1839. 1840 Standard Staphylococcus HiVeg Broth. 1632 Staniers Basal Medium with Pyridoxine and Yeast Extract. 1632 Staniers Basal Medium with Trichlorophenoxyacelate, 1633 Staph'Strep Selective Supplement, 7 Staphylococcal enterotoxin, 232,245 Staphylococcal food poisoning, 1538 Staphylococcal species. 245 Staphylococci, 162,192,193.194,227,280. 345,649,657,908.909,991,1006,1007, 1216,1242.1393, 1538. 1633,1691, 1804

Staphylococcus Agar No. 110. 1633 Staphylococcus arlettae, 454 Staphylococcus aureus, 16. 122, 127. 131, 193, 332, 377,433, 434,454,463, 644, 655.657.740.811,812,814.953.1157. 1236, 1302, 1330, 1392,1407, 1408, 1429. 1525.1633. 1634.1800. 1831. 1833.1844,1845. 1846.1892.1893 Staphylococcus aureus Enrichment Broth, 601 Staphylococcus aureus Enrichment HiVeg Broth, 1633 Staphylococcus aurictdaris. 454 Staphylococcus Broth. 1633 Staphylococcus capitis. 454, 1429 Staphylococcus caprae, 454 Staphylococcus carnosus. 454, 1470. 1835, 1836 Staphylococcus caseolyticus. 454, 1933 Staphylococcus chrtmwgenes, 454 Staphylococcus cohnii. 454, 1429 Staphylococcus delphini, 1429 Staphylococcus epidermidis. 454,638. 644 Stuj)hyhcoc'cus equorum, 454 Staphylococcus gallinarum, 454 Staphylococcus haemolyticus. 454 Staphylococcus hominis, 454 Staphylococcus hyicus, 454 Staphylococcus intermedins, 454 Staphylococcus kloosii. 454 Staphylococcus lentus. 454, 1429 Staphylococcus lugdunensis, 1429 Staphylococcus Medium. 1634 Staphylococcus inttscae, 1429 Staphylococcus saccharoiyticus, 1459, 1848 Staphylococcus saprophytics, 454 Staphylococcus schleiferi, 1429 StaphyloccKcus sciuri, 454 Staphylococcus simuians, 454, 1429, 1584 Staphylococcus species, 164,285, 433. 434, 454,463, 603, 604, 633, 673,814, 1311, 1338. 1470,1551, 1814 Staphylococcus warneri, 454 Staphylococcus xylosus. 454. 1429 Staphylococcus Streptococcus Selective Medium. 1634 Staphyhthermus hellenicus. 1639 Staphyhthermus marinus, 1467 Starch. 1033 Starch Agar, 1634 Starch Agar Medium for 1'settdomonas. 1635 Staich Agar with Bromcresol Puqile, 1635 Starch Casein Agar. 1635 Starch Casein Potassium Nitrate Agar, 1636 Starch fermentation, 1391 Starch Fermentation Broth, 1636 Starch HiVeg Agar. 1636 Starch hydrolysis, 679, 680, 1526,16.34, 1635. 1636 Starch 1 lydrolysis Agar, 1636 Starch Medium. 1637 Starch Mineral Salt Agar, 1637

Index

Starch Nitrate Medium, 1637 Starch Salts Agar, 1637 Starch utilization. 76. 1952 Starch utilization, 1930 Starkey's Medium C, Modified, 1637 Slarkey's Medium C. Modified with Salt, 1638 Starter cultures, 986 Stccnkcn and Smith Agar. 1638 Stella species, 330, 1463 Stenotrophomonas maltophilia. 1171. 1916. 1950 Stephanoascus species. 1001 Slephanoeca diplocostata, 1015 Stephanopogon apogon, 1015 Stereocauhn vulcani. 951 Sterility, 1631.1632 Sterility Test Broth, 1638 Sterility testing. 17. 18.47. 256. 391.645. 694.695, 1002, 1289. 1344. 1533. 1639, 1772.1774,1775. 1843 Steroidolxicier denitrificans, 1649,1650 Sleroidobacler Medium with I leptanoate, 1649 Steroidobacter Medium with Testosterone, 1648 Slerolibacterium denitrificans DSM 13999, 103 Stetteria hydrogenophila. 1639 Sletteria Medium, 1639 Stigmatelia aurantiaca, 466, 1594 Stigma tella erecta, 1594 STL Broth, 1639 Stock Culture Agar, 161, 1640 Stock Culture Agar with i.-Asparagine, 1640 Stokes Agar. 1640 Stomatococcus muciiaginosus, 454 Stonebrink's Medium, 1640 Stool specimens, 1598 Straw DYAA. 1640 Straw Malt Agar. 1641 Streak method, 1645 Strep (Streptococcus) ID (Identification) Quad Plate, 1641 Strep ID Quad Plate. 1641 StrepB Carrot Broth, 1641 Streptacidiphilus jiangxiensis. 884 Strq/loalloteichus species, 1863 Streptobacilltis moniliformis. 814, 816. 854 StrejUobacillus species, 816 Streptococcal Growth Medium. 1641 Streptococcal mutants, 986,987 Streptococci. 101. 103. 104. 137, 138. 162. 163. 191, 192,201,218,219,220,227, 228.229. 230.244,266.290.291.375. 466. 597,630.635.636.649.650.655. 720, 760, 761, 881, 896, 897, 898,918, 919.920. 970.986.1026. 1069. 1140. 1242. 1283, 1307, 1393,1397, 1484. 1485.1537,1570. 1634,1640, 1641, 1642. 1643. 1644. 1648.1800, 1847, 1848. 1849, 1850, 1851.1869,1881

Streptococcus acidominimus. 454. 1429 Streptococcus agatactiae, 378, 630, 744, 1826, 1827, 1835 Streptococcus agalactiae Selective HiVeg Agar Base with Blood and Staphylococcus B toxin, 1641 Streptococcus Agar, 1642 Streptococcus alactolyticus, 455. 1429 Streptococcus albogriseolus. 1645 Streptococcus anginosus. 1429, 1933 Streptococcus B. 378 Streptococcus Blood Agar. Selective. 1642 Streptococcus hovis. 216. 454, 679. 680, 1652,1653 Streptococcus can is, 454, 1429 Streptococcus constellatus, 1459 Streptococcus cricetus. 1429 Streptococcus cristatus, 1800 Streptococcus downei. 1429 Streptococcus dysgalactiae, 441, 1429 Streptococcus Ijnichmeiu HiVeg Broth, 1642 Streptococcus equi, 1429 Streptococcus equinus. 216. 454, 679.680 Streptococcus equismilis, 744 Streptocixrcus faecal is. 1567. 1702. 1802 Streptococcus faecaiis Broth, 1567 Streptococcus faecium. 1567 Streptococcus ferus. 1429, 1945 Streptococcus gordonii, 1429, 1828 Streptococcus hansenii, 370, 1459 Streptococcus Imiintestinalis, 1429 Streptococcus iniae, 214 Streptococcus intenncdius. 1459 Streptococcus intestinalis, 454 Streptococcus tactis, 987, 1468 Streptococcus laclis Differential 1 liVeg Agar Base with Potassium I'erricyanide and Citrate, 1642 Streptococcus lactis subspecies diacctyiactis, 1642 Streptococcus macacae, 1429 Streptococcus Medium, 1643 Streptococcus mitis, 1191. 1429 Streptococcus /nutans, 131.453, 744, 747. 1197, 1429. 1485, 1643, 1652.1653, 1892,1932 Streptococcus /nutans Medium. 1643 Streptococcus oralis, 454 Streptococcus orisratti. 215 Streptococcus parasanguis. 1429 Streptococcus parauberis, 1429 Streptoaxrcus pleomorphus. 1363, 1459 StreptoctKCUs plulon, 1645 Streptococcus pneumoniae, 162,440, 1250, 1429,1643, 1823, 1827, 1828 Streptococcus pneumoniae Medium. 1643 Streptococcus porcinus, 1429 Streptococcus pyogenes. 214,251.744, 761. 1283,1311, 1429, 1881

2023

StrephKoccus rattus. 1429 Streptococcus salivarius, 454, 744, 1191, 1197, 1429.1652 StrepUn-occus sanguis, 454, 1197, 1429, 1485,1652.1653.1892 Streptocixcus Selection 1 liVeg Agar, 1643 Streptococcus Selection I liVeg Agar with Cycloheximide, 1643 Streptococcus Selection HiVeg Broth, 1643 Streptococcus Selective Medium. 1644 Streptococcus Selective Supplement COA, 7 Streptococcus sobrinus, 1429. 1945 Streptococcus species, 164,454,644, 656, 693, 694. 744, 970. 984. 1140. 1429, 1538, 1567,1641, 1642, 1702, 1803, 1888 Streptococcus suis, 1644 Streptococcus suis Medium. 1644 Streptococcus thermophilic 860, 988. 1644 Streptococcus thermophilic Agar, 1644 Streptococcus thenuophilus Isolation IliVeg Agar, 1644 Streptococcus uberis, 1429. 1644. 1823 SlreptociKcus uberis Broth. 1644 Streptococcus witibularis, 1429 Streptomyces aculeolatus, 1517 Streptomyces Agar. 1644, 1645 Streptomyces alhospintts, 1939 Streptomyces aibus, 639 Streptomyces anlibiolicus. 1929 Streptomyces argenteolus, 1929 Streptomyces armeniaeus, 480 Streptomyces aureofaciens, 1929 Streptomyces avermtlilis, 743, 928, 1800 Streptomyces badius, 1645 Streptomwes bikiniensis. 1645 Streptomyces hluensis, 1929 Streptomyces cacaoi. 210 Streptomyces caeleslis, 1929 Streptomyces calms. 743 Streptomyces canus, 1637 Streptomyces capillispiralis. 1318 Streptomyces chartreusis, 1517, 1669 Streptomyces cinereus, 1921 Streptomyces cinnamoneus. 1929 Streptomyces cyanogriseus, 1637 Streptom\>ces cystai-gineus. 1939 Streptomyces echinatus, 1929 Streptomyces flaveus, 638, 1410, 1411, 1921 Streptomyces flocculus, 215 Streptomyces fradiae. 1416, 1637 Streptomyces fragmentans, 908 Streptomyces fragmentosporus, 1191 Streptomyces griseocarneus, 1929 Streptomyces griseus. 743, 868, 978, 1645, 1929 Streptomyces Streptomyces Streptomyces Streptomyces Streptomyces Streptomyces

hawaiiensis. 1929 liinxshimeniis, 1637 hygroscopicus. 743 kamnnyceticus, 1645. 1929 kentucke/isis. 1929 kuwaitiensis, 1637

2024

Index

Streptom\x.vs laclamdurans, 743 Streptomyces Hvidans, 1525, 1941 Streptomyces longwoodensis, 1939 Streptomyces macrosporeus, 1363 Streptomyces Medium, 1645 Streptomyces meiachromogenes, 1323 Streptomyces murinus, 1929 Streptomyces naniwwensis, 1230 Streptomyces nefropsis, 1929 Streptomyces nivetis, 1929 Streptomyces nobilis, 1416 Streptomyces nogalater, 1929 Streptomyces nq/iriensis. 1318 Streptomyces nousei, 1929 Streptomyces olivaceus, 638 Streptomyces paucisporogenes, 1929 Streptomyces peucetius, 886 Streptomyces phaeopurpureus, 719 Streptomyces piedadensis. 1309 Streptomyces prasinosporus. 1416 Streptomyces pulveraceus, 1318 Streptomyces purpureus. 886, 887. 1320 Streptomyces rectus, 1587 Streptomyces rimosus. 1363. 1929 Streptomyces rubroverrucosus, 1637 Streptomyces sannurensis, 853 Streptomyces scabies, 1762 Streptomyces sparsogenes, 1929 Streptomyces species, 210, 216, 284, 321, 456.480. 521. 732.752. 753.767.848. 884, 885, 886, 887, 1300, 1318, 1321, 1322,1323.1416,1618.1645, 1862. 1863,1899, 1915, 1929,1934, 1939. 1943 Streptomyces spectabdis, 1929 Streptomyces xpinoverrucosus, 1637 Streptomyces sfwroverrucocus, 1939 Streptomyces tendae. 1929 Streptomyces tenebraruis, 1930 Streptomyces testaceus, 1412 Streptomyces tbennoautotrophicus, 58 Streptomyces thennodiaslafiats. 1517 Streptomyces ihennogriseow'olaceus, 469 Streptom\>ces fhermobygroscopicus. 469 Streptomyces thermoviolaceus, 638 Streptom\>ces thermovtdgaris. 469. 638 Streptomyces tosaensis, 743 Streptomyces vendargcnsi. 638 Streptomyces venezuelae, 1376 Streptomyces violaceoruber, 1930 Streptomyces viridifaciens, 1930 Streptomyces viridoverrucosus, 1637 Streptomyces yerevanensts, 481, 758 Slrcptomycelaceae. 638 Streptomycete Antibiotic Activity Inoculum Medium, 1645 Streptomycete Antibiotic Activity Medium, 1645 Streptomycete Medium. 1646 Streptomycetes, 349, 752,1334,1344,1636. 1646.1733. 1863

Streptomycin, 1776,1779

Streptomycin Assay Agar with Yeast Extract, 122,131 Streptomycin HiVeg Agar with Yeast Extract), 127 Streptomycin I. Broth Medium. 1646 Streptomycin Nutrient Agar, 1647 Streptomycin Nutrient Agar No. 2. 1647 Streptomycin Nutrient Agar No. 3,1647 Streptomycin Nutrient Agar No. 4, 1647 Streptomycin Terramycm* Malt Extract Agar, 1647 Streptomycoides glaucoflavus, 1320. 1322 Streptosclâ&#x201E;˘ Agar. 1648 Streptoselâ&#x201E;˘ Broth, 1648 Streptosporangium alhum, 1322, 1411, 1517 Streptosporangium amethystogenes, 1321 Streptosporangium corrugation, 767 Streptosporangium fragile, 1929 Streptosporangium koreanum, 1319 Streptosporangium longisporum. 638 Streptosporangium pseudovulgare, 1323 Streptosporangium roseum, 1321 Streptosporangium roseum subsp. incarnatum, 1319 Streptosporangium sibiricum. 1323 Streptosporangium species, 886, 1318, 1939 Streptosporangium violaceochromogenes. 1321 Streptosporangium viridialbum. 1939 Streptoverticillium baldaccii, 1414 Streptoverticillium morookaense. 743 Streptoverticillium species, 886, 1618, 1637, 1934 SIT Agar, 1654 SITA Medium. 1650 Stuart Leptospira Broth. Modified, 1650 Stuart Medium Base, 1650 Stuart Transport Medium, 1650 Stuart Transport Medium, Modified, 1650 Stygiolobus azoricus. 1645,1651 Stygiolohus Medium, 1651 Styrcnc Mineral Salts Agar, 1651 Styrene Mineral Salts Broth, 1651 Styrene utilization. 1651 Suboxydans, 1342 Succinate Mineral Medium. 1651 Succinate utilization, 1652 Sueciniclasticum Medium, 1652 Succiniclasticuin ruminis, 1652 Succinimonas atnylolytica. 339. 1489,1522 Succinivibriodextrinosolvens, 616, 617, 1369, 1522 Succinomonus atnylolytica, 1419 Succinovibrio dexlrinisolvens. 1419 Sucrose Agar. 1652 Sucrose Broth, 1652 Sucrose Fermentation, 1885 Sucrose fermentation, 817, 818, 1391, 1654, 1819.1820.1918.1919 Sucrose HiVeg Agar lor Brewery Isolates. 1653 Sucrose Peptone Agar. 1653

Sucrose Peptone Medium, 1653 Sucrose Phosphate Glutamalc Transport Medium, 1653 Sucrose Phosphate Transport Medium, 1653 Sucrose Salicin Agar. 740 Sucrose Teepol Tellurite Agar, 1654

Sucrose utilization, 1654 Sucrose Yeast Lxtract Medium. 1654 Sucrose-fermenting. 260. 261. 262 Sudan Black B, 1406 Sugar fermentation. 903. 904 Sugarcane, 1548 Suillns species, 1199 Sulfamandclatc Supplement, 7 Sulfate API Broth, 1654 Sulfate reduction. 1654, 1900 Sulfale-reducing bacteria, 1655, 1656, 1657 Sulfatc-Roducing Bacteria Enrichment Medium, 1654 Sulfatc-Rcducing Bacteria Medium. 1655 Sulfate-Reducing Bacteria Med nun with Lactate, 1656 Sulfate-Reducing HiVeg Medium. 1657 Sulfale-Redueing Medium, 1657 Sulfite Agar, 1657 Sulfite HiVeg Agar with Iron, 1658 Sulfite Polymyxin Sulfadiazine Agar, 1618 Sulfite reduction, 1658 Sulfiiobacter brevis, 635 Sulfitohacler mediteiraneus, 1009 Sulfiiobacter pontiacus. 1658 Sulfiiobacter pontiacux Medium, 1658 Sulfobacillus acidophilus, 51. 52 Sulfobacillus disulftdooxidans. 1659 Sulfobacillus disulftdooxidans Medium, 1658 Sulfobacillus Medium. 1659 Sulfobacillus sppecies, 676 Sulfobacillus thermosulfidooxidans, 1659 Sulfobcnzoic acid utilizing bacteria, 1183 Sulfolobus acidocaldarius, 1659, 1660 Sulfoiobus acidocaldarius Simplified Basal Medium, 1659 Sulfolobus hrierleyi Medium. 1659

Sulfolobus Broth, 1659 Sulfolobus Medium, 1660 Sulfolobus Medium, Revised, 1660 Sulfolobus shibatae, 1661 Sulfolobus shibatae Medium. 1661 Sulfolobus solfataricus, 1661 Sulfolobus solfataricus Medium, 1661 Sulfolobus species, 1660, 1661 Sulfonamide therapy. 252 Sulfonamides, 133, 1564, 1776,1779 Sulfophobococcus zilligii. 1661 Sulfophobococcus zilligii Medium, 1661 Sulforhabdus Medium, 1661 Sulfur, 880, 1005, 1662,1785 Sulfur bacteria. 323.997. 1552, 1657 Sulfur Medium. 1662 Sulfur source, 1770 Sulfuricunvm kujiense, 1031

Index

Sulfurihulrogenibium azorense. 861 Sulfurihydrogenihium kristjamsonii, 1241 Sulfurihytlrogenibiumsubterraneum. 1196 Sulfurinumas autotrophica, 1193 Sulfurimonas panilvinella. 1663 Sulfurimonas paralvinella Medium, 1662 Sulfurivirga caUiicttralii. 1198 Sulfuraspirillum arsenophilum, 1665 SulfurospiriUum barnesii. 1665 SulfurospiriUum deleyiunum, 1663, 1664 SulfurospiriUum halorespirans, 491 SulfurospiriUum halorespirans DSM 13726, 492 SulfurospiriUum 11 Medium. 1664 SulfuraspiriUum Medium, 1663 SulfurospiriUum MV Medium. 1665 SulfurospiriUum species, 1043, 1666 Super HiVeg Broth. 1666 Super MMB Medium, 1666 Supcrbrotlu 1666 Supplement A, 5 Supplement B, 5 Supplement C, 5 Supplement VX, 5 Supplemented Aspergillus Minimal Agar, 1666 Supplemented Aspergillus Minimal Broth. 1667 Surface sterility, 1631. 1632

SW,2Agar, 1667 SWA, 1553. 1554 Swab specimens, 90,1807 Swampy Medium, 1667 Sweet I- Broth tor Anaerobes, 1667 Swimming pool water. 953. 1168. 1634 Swimming pools, 857 Swine. 1274 SWM Medium, 1668 SWMTY Marine Medium, 1668 SX1 Blood Agar. 1669 SY Broth, 1669 SYA Medium. 1669 SYC Medium, 1669 SYFAC Medium, 1669 SYI.C Medium, 1670 Syncasc Broth, 1671 Synechocaccus species, 213, 214, 233, 1200 Synechocystis species. 213. 214, 1200 Synthetic Broth. AOAC, 1671 Synthetic Broth. Association of Official Analytical Chemists, 1671 Synthetic Complete Medium, 1671 Synthetic Malate Medium with 0.25% Sodium Chloride, 1672 Synthetic Mucor Agar, 1672 Syntlietic Sea Salt, 1672 Synthetic Seawatcr Medium. 1672 Syntrophohucter huswellii, 1541 Synirophobacler pfennigii, 1673 Syntrophohucter pfennigii Medium, 1672 Synirophobacler species, 1230 Syntrophohucter wolinii, 1542, 1674

Syntrophobiicler wolinii Medium. 1673 Syntrophohotulus species, 689 Syntroplwcoccus sucromutans. 1674 Syntrophococcus sucromutans Medium, 1674 Syntrophomonus hryanlii Medium, 1674 Syntwphomonas Medium, 1675 Syntrophomonus Medium, Sulfate-Free. 1676 Syntrophomonus sapovorans. 1675 Syntrophomonus sj>eeies, 1676 Syntrophomonus species Medium. 1677 Syntroplwmonas wolfei, 1678 Syntrophospora bryantii. 399, 400 Svntrophothennus lipocalidus DSM 12680, 1679 Svntrophothennus lipocalidus DSM 12681, 1679 Svntrophothennus Medium, 1678. 1679 Syntrophus buswelii. 1677, 1680, 1681, 1682 Syntrophus buswellii II Medium. 1679 Syntrophus Medium, 1680 Syntrophus Medium Sulfate l:rcc, 1681 SYPC Medium,, 1682 T

T, 7 Agar Base, 1682 T.S.CJS.F.P. Hi Veg Agar Base, 1371 T,N 0 Broth. 1798 T,N, Agar, 1798 T,N, Broth, 1798 T,N 2 Agar, 1798 T,Nj Broth, 1798 T,N 6 Broth, 1798 T,N S Broth. 1798 T,N, 0 Broth. 1799 T2 Medium for Thiohacillus, 1682 13 Agar. 1750 T3 Broth, 1751 Talaromyves ucrainicus. 1935 lap Water Agar, 1683 Taphrina californica, 348

Taphrina populina. 348. 1949 Tarshis Blood Agar, 1683 Tartoff-Hobbs IliVeg Broth with Glycerol, 1683 Tartrate fermentation, 1392 T-ASW Medium. 1682 TAT Broth Base, 1683 I AT HiVeg Broth Base with Polysorbate. 1684 Tatlockia micdadei. 471 Talumellu plyseos, 215 Taurocholate Tellurite Gelatin Agar, 1222 Taxeobacter S|>ecies, 1272 Taylorella equigenitalis, 663.664, 721, 774 TB Broth Base, 1684 TB Broth Base without Polysorbate. 80.1684 IB HiVeg Broth Base with Bovine Albumin and Glucose, 1684 TB I IiVeg Broth Base with Bovine Scrum and Glucose, 1685

2025

IB Hi Veg Broth Base without Tween 80 with Bovine Albumin and Glucose, 1685 TB HiVeg Broth Base without Tween 80 with Bovine Serum and Glucose, 1685 TB Nitrate Reduction Broth. 1685 THAU, 298 Medium. 1686 TBX Agar. 1686 TOY A Agar, 1686 TC Amino Acids. HcLa. 100X. 1789 TC Amino Acids, Minimal l-agle, 50X, 1789 TC Dulbecco Solution. 1790 TCIiarle Solution. 1790 TC Hanks Solution. 1790 TC Medium. 199, 1790 TC Medium Eagle with Farle BSS, 1791 TC Medium Faglc with Hanks BSS. 1791 TC Medium I agle.Hel.a. 1792 TC Medium Ham 1-10.1792 TC Medium NCTC, 109, 1793 TC Medium RPM1 #1640.1793 TC Minimal Medium l-agle Spinner Modified MKM-S. 1795 TC Minimal Medium liagle with l!arle BSS, 1794 TCTyrode Solution. 1795 TC Vitamins Minimal l-agle, 100X, 1795 TCBS Agar. 1687 TCBS HiVeg Agar, 1687 TCBS HiVeg Agar (Selective). 1687 TCG Medium,, 1687 TCH Medium. 1688 TCN Medium, 1863 TCY Agar. 1688 ICY Broth, 1688 TD3 Medium. 1688 TDC Medium, 1689 Tea fungus. 1690 Tea Fungus Medium. 1690 TFC Agar. 1690 Tech Agar. 1690 Teepol Broth, Fnriched, 1690 Tccpol Hi Veg Broth, 1690 Tellurite Blood Agar, 362 Tellurite Glycine Agar, 1690. 1691 Tellurite Polymyxin l-gg Yolk Agar, 1803 Tellurite Solution, 7 TEP Uric Acid Medium, 1691 Tepidanaerobacter Medium., 1691 Tepidanaerobacter synfroplncus* 1692 Tepidibacter Medium with Peptone. 1693 Tepidibacler Medium,, 1692 Tepidibacter thalassicus. 1693 Tepidimonas ignava, 1747 Terasakiella pusilla, 1009 Teredinobacler Medium, 1693 TergitoT™ 7 IliVeg Agar Base with ITC, 1695 TergitoT™ 7 IliVeg Agar H, 1695 Tcrgitol™ 7 Agar. 1694 Tergitol1" 7 Agar 11,1694 Tergitol IM 7Broth. 1695 Tergitol™ 7 IliVeg Broth, 1695

2026

Index

Termttobacter Medium. 1695 Tcrrabacter tumescens. 454, 1470 Terrific Broth. 1696 Terrific Broth with 100 mg/ml Ampicillin, 1696 Terrific Broth with lOOmicrogram/ml Ampicillin. 1470 Terrific Broth with 200 microgram/ml Ampicillin. 1696 Terrific Broth with 50 nig/ml Ampicillin. 1696 Terrific Broth with 50 microgram/ml Ampicillin, 1470 Terrific HiVeg Broth. 1683 Test, 91 Test organism. 171. 172 Tetracycline I. Broth Medium, 1697 Tetracycline I.uria Agar No. I. 1697 Tetracycline I.uria Agar No. 2. 1697 Tetracycline I.uria Agar No. 3. 1698

Tetracycline TY Salt Medium, 1698 Tetracyslis disociuta. 70 Tetmgenococcus hahphih. 1236. 1944 Tetmgenococcus halophilus, 23, 769.1351 Tetragenococcus murialicus, 769 Tetrahymena australix, 808 Tetmhymena hegewischi, 1698 Tetrahymena hyperangularis. 1698 Tetrahymena limacis, 1704 Tetrahymena malaccenxis, 1698 Tetrahymena Medium. 1698 Tetrahymena mimbres. 1698 Tetrahymena nanney, 1698 'Tetmhymena nipissingi, 1698 Tetrahymena patula. 962. 1704 Tetrahymena pigmentosa. 1698 Tetrahymena pyriformis. 1698 Tetrahymena rostrata, 1704 Tetrahymena selosa, 1704 Tetmhymena siluina. 1698 Tetmhymena sonneborni. 1698 Tetmhymena species, 1217 Tetmhymena thermophda, 1698 Tetrahymena tropical is, 1698 Tetmhymena vora.x, 808 Tetramethyl Ammonium Chloride Agar, 1698 Tetmmitus rostralus, 1454, 1699 Tetramitus rostmtus Flagellate Medium, PY/ 2, 1698 Teimsphaera elongata, 1513 TeUathionale Brilliant Green HiVeg Broth, 1699 TeUathionale Broth, 1699, 1700 Tetrathionate Broth Base without Iodine & BG. 693 Tetrathionate Broth with Novobiocin, 1700 Tetrathionate Broth, Hajna, 1700 Tetrathionate Broth. USA. 1700 Tetrathionate Crystal Violet Enhancement Broth, 1701

Teualhionate HiVeg Broth Base without Iodine & BG, 692 Tetrathionate I IiVeg Broth Base. I lajna with Iodine, 1701 Tetrathionate Reductase Medium, 1701, 1762 Tetrathionate reductase production, 1701 Tetrathionate Reductase 'Test Medium, 1702 Tetrathionate reduction, 1702 Tctratrichomonas gallinanim. 467, 1861 Tetra/olium Thallium Glucose Agar, 1702

'Tetra/olium tolerance, 1702 Tetra/olium Tolerance Agar. 1702 Textiles. 16 TF Medium. 1702 TI'(A) Medium, 1703 TGA Medium, 1704 TGB HiVeg Agar. 1704 TGE Broth, 1704 IX JMM-2 Medium, 1704 TGY Medium, 1704 TGYM Medium, 1704 Thalassomonas viridans, 1009 Thanatephorm cucumeris, 775 Thauera aromatica, 1705, 1706 Thauera aromatica AR-1 Medium. 1705 Thauera aromatica Medium. 1706 Thauera mechernichensis, 1707 Thauera mechernichi Medium, 1707 Thaumatomastix species, 1589 Thavem pivativorans DSM 14691. 1209 Thaxtera salina, 1899 ITiayer-Martin Agar. Modified, 1707 Thayer-Martin HiVeg Medium Base with I lemoglobin and Vitox Supplement, 1707 Thayer-Martin Medium, 1708 'Thayer-Martin Medium, Modified, 1708, 1709 Thayer-Martin Medium, Selective, 1709 Iliayer-Martin Selective Agar. 1710 Thelephora lerrestris, 775. 1270 Thermacetogenium phaeum, 1712 Thermanaeromonas toyohensis, 1718 Thermanaerovibrio veUxc, 1718 Thermicanus aegyptius, 1616 Thermincola carboxydiphila, 1711 Thermincola Medium,, 1710 Thermoacelogenium phaeum Medium. 1711 Thermoacidumns Agar, 1712 Thermoacidurans HiVeg Agar, 1712 Thermoactinomvces candidus, 469, 1363. 1630 Thermoactinomvces dichotomicus, 210, 767, 768. 1363,1637. 1712 Thermoactinomvces dichotomicus Medium. 1712 Thermoactinomvces glaucus. 210. 1330 Thermoactinomyces intermedins, 1363, 1429 ThernuKicfinomyces Mediun\ 1712, 1713 Thermoactinomyces pepionoplulus, 1713

Thermoactinomyces putidus, 469, 1320. 1321,1363 Thermoactinomvces sacchari, 469, 1517, 1713 Thermoactinomvces species, 469, 1618,, 1713 Thermoactinomvces thalpophilus, 469, 481, 1363 Thermoactinotnyces vulgaris. 469.481, 1321, 1323,' 1363, 1410,1411, 1517, 1630 Thermoactinopolyspora Medium, 1713 Thermoaclinopolyspom species, 1713 Thermoanaerobacter elhanolicus, 425 Thermoanaerobacter elhanolicus Medium, 1713 Thermoanaerobacter italicize, 907 Thermoanaerobacter species, 1714 Thermoanaerobacter subterraneus. 1714 Thermoanaerobacter subterraneus Medium, 1714,1716 Thermoanaerobacter sulfumphilus Medium. 1714,1917 Thermoanaerobacter tengcongensis. 1716 Thermoanaerobacter tengcongensis Medium, 1715 Thermoanaerobacter thermohyxlrosulfuricus, 425 Thermoanaerobacterium Medium. 1716 I hermoanaerobacferium polysaccharolyticwn, 1716 Thermoanaerobacterium thennosulfurigenes. 427 Thermoanaerobacterium zeae, 1716 Thermoanaerobium brockii. 198, 678, 1717 Thermoanaerobium brockii Medium. 1716 Thermoanaerobium crenophilum. 1338 Thermoanaerobium olidum, 1338 Thermoanaeromonas Medium. 1717 Thermoanaerovihrio Medium, 1718 Thermobaclerium Medium, 1719 Thermolxicieroides acetoethylicus, 1075 Thermobacteroides leptospartum, 457, 1719 Thermobacteroides leptospartum Medium. 1719 Thermolxicteroides proteolyticus Medium. 1719 Thermococcus acidaminovorans DSM 11096,83 Thermococcus acidophilum, 1733, 1734 Thermococcus aegaeus, 1020 Thermococcus aggregans, 1022 Thermococcus alcaliphilus DSM 10322. 82 Thermococcus celer, 1720 Thermococcus celer DSM 2476, 1021 Thermococcus celer Medium, 1720 Thermococcus chilinophagus. 1721 Thermococcits chilinophagus Medium. 1720 Thermococcus fumicolans, 1022 Thermococcus gorgonanus, 1020 Thermococcus guaymasensis, 1022

Index

Thermococcus litondis. 1011.1013.1022, 1721, 1722 Thermococcus litondis Medium. 1721 Thermococcus marinus, 1951 Thermococcus Medium. 1722 Thermococcus pacificus, 1020 Thermococcus profundus, 1022, 1723 Thermococcus profundus Medium, 1722 Thermococcus sibiricus. 1022 Thermococcus species. 96, 1722 Thermococcus stetteri. 1723. 1854 Thermococcus stetleri DSM 5262, 1021 Thermococcus stetteri Medium, 1723 Thermococcus waiotapuensis. 780 Therm<x:rmis ruber, 1331 Thermocrispum agreste, 1630 Thermocrispum municipale, 1630 Thermodesulfobacterium commune. 1724. 1726 Thermodesuifobaeterium Medium. 1723 Thermodesulfobacterium mobile, 562 Thermodesulfobacterium species, 174, 1726 Thermoilestdfobium Medium,, 1724 Thermodesulfohium narugense, 1724 Thernnxlesulforhabdus Medium, 1724 Thermodesulforhabdus norvegicus. 1725 Thermotlesulfotobacterium Agar, 1725 Thermodesulfotobacterium Broth, 1726 Thermotlesulfovibrio yellowstonii. 1727. 1949 Thermodesulfovibrio yvllowstonii Medium, 1726,1738 Thermodiscus marifimus, 1581 Thermofdum librum. 1878 Thermofdum pendens, 1728 Thermofdum pendens Medium. 1727 Thermogymnomonas Medium. 1728 Thermogymnomonas species, 1728 Thermoleophilum album, 912, 1728 Thermoleophilum Medium. 1728 Thermoleophilum minuium. 912, 1728 Thermomicrobiumfosteri, 1728. 1729 Thermomicrohium fosteri Agar, 1728 Thermomicrobiumfosteri Broth, 1728 Thermomicrohium roseum, 1719, 1729 Thermomicrobium roseum Agar. 1729 Thermomicrohium roseum Medium, 1729 Thermomonospora alba, 1729 Thermomonospora aquaticus, 1857 Thermomonospora chromogena, 210,481, 1304.1315 Thermomonospora cun'ata, 832, 1322 Thermomonospora formosensis, 1637 Thermomonospora fusca, 469, 1859 Thermomonospora Medium. 1729 Thermomonospora mesophila, 1322, 1645, 1729 Thermomonospora mesouvi/brmis, 886 Thermonema lapsum. 328 Thermonema rosstanum, 1747 Ihermophilic actinomycctcs, 1063 Thermophilic Bacillus Medium, 1729

Thermophilic Bacillus species, 1040 Thermophilic Ilydrogen-Baeteria Medium, 1729 Thermophilic Maintenance Medium, 1730 Thermophilic Methanosarcina Medium, 1730 Thermophilic Methanothrix Medium. 1731 Thermophilic species, 326, 327 Thermophilic Spirochete Medium, 1732, 1733 Thermophilic Streptomyeete Medium, 1733 Thermophilic Streptomyeete Medium IA, 1733 Thermophilic streptomycetes, 1733 Tlietmophilus species, 1764 Thermoplasma acidophilum. 1734 Thermoplasma acidophilum Growth Medium. 7B. 1733 Thermoplasma acidophilum Medium. 1733 lliermoplasma acidophilum Medium 7A, 1734 lliermoplasma Agar, 1734 Thermoplasma Broth. 1734 lliermoplasma species, 1734 Thermoplasma volcanium. 1735 Thermoplasma volcanium Medium, 1734 lliermoproteus Medium, 1735 lliermoproteus neutrophils, 1735 Thermoproteus neutrophilus Medium, 1735 Thermoproteus species, 1735 lliermoproteus tenax, 1735 lliermoproteus uzoniensis, 572 lliermosinus velox. 1718 lliermosipho africanus, 1736 lltermosipho africanus Medium, 1735 lliermosipho species, 1703 lliermosphaera aggregans, 1736 lliermosphaera Medium. 1736 lliermosuljidibacler species, 1737 Thermosyntropha lipolytica. 1738 lliermosyntropha Medium, 1737 Thermoterrabaeteriumferriredueens. 1739 lliermoteirahacterium Medium, 1738 lliermotoga 2 Medium. 1741 lliermotoga elfti, 1739, 1740 lliermotoga elfii Medium, 1736. 1739 lliermotoga hypogea, 1741 lliermotoga hypogea Medium. 1740 lliermotoga maritima, 1741 lliermotoga Mali urn. 1741 lliermotoga naphthopliUa. 1742 lliermotoga neapolitana, 1199, 1741 lliermotoga petrophila. 1742 lliermotogapetrophila Medium, 1741 lliermotoga species, 1104 lliermotoga subterranea> 1743 lliermotoga subterranea Medium. 1742 lliermotoga thermarum, 1741 Thermovenabulumferriotganovorum, 1743 lliermovenabtdum Medium, 1743 lliermovibrio Medium. 1743 lliermovibrio ruber, 1744

2027

Thermits 162 Medium 1746, 1747 Thennus Agar, 1744 Tliermus aquaticus. 327, 1047. 1744. 1746, 1748 Thennus Beef Extract PolypcptoncIM Medium, 1744 Thennus BP Medium. 1744 Thermus brockii, 1745 Thennus brockii Medium, 1744 Thermus Broth, 1745 Thennus Enhanced Agar. 1745 Thermus l-jihanced Agar with 1% Sodium Chloride, 1745 Thermus fdiformis, 1857 Thennus flavus, 1857 Thennus Medium, 1746 Thermus Medium Mnlianced, 1747 Thennus Medium hnhanced with 1% NaCl, 1748 Thennus Peptone Meat Mxtnict Yeast Ivxtracl Agar. 1748 Thennus Peptone Meat Extract Yeast loaracl Broth. 1748 Thennus PMY Agar, 1748 Thennus PMY Broth, 1748 Thennus ruber, 1417, 1748, 1857 Tliermus ruber Medium. 1748 Thennus sp. Medium, 1748 Thennus species. 328. 1046. 1744. 1745. 1746, 1747,1748,1857 Thennus thennophilus, 1749 Tliermus thermophilus Medium. 1748 Thialkalimicrobium aerophilum, 82 Thiulkalimicrobium sibiricum. 82 Thialkalivibrio paradoxus |)SM 13531, 79 Thialkalivibrio paradoxus DSM 13542.80 Thialkalivilwio sp. DSM 13533, 80 Thialkalivibrio thiocyanoxidans DSM 13532,79 Thialkalivibrio thiocyanoxidans DSM 13541,79 Thiamine assay, 1749 Thiamine Assay Medium, 1749 Thiamine Assay Medium LV. 1749 "Thiamine Salts Medium. 1749 Thia/ole Medium. 1749 Thielavia hwlocarpa. 1004 Thiela\ia terricola, 944 THIO + Bile Medium, 1772 THIO Medium. 1773 Thioalkaiimicrobium aerophilum, 82 Thioalkalimicrobium sihericum, 82 Thioalkalivibrio denitrificans. 81 Thioalkalivibrio halophilus, 1750 Thioalkalivibrio halophilus Medium, 1750 Thioalkalivibrio versutus DSM 13738, 81 Viioalkalivibrio versutus DSM 13741. 81 Thiobacilli, 1683 Thiobacillus A2 Agar. 1750 Thiolxicillus A2 Broth. 1751 lliiobacillus acidophilus, 743, 744, 1751 Thiobacillus acidophilus Agar, 1751

202S

Index

Thiobacillus acidophilus Broth, 1751 IhiobaciUus acidophilus Medium, 1751, 1752 Thiahacillus Agar, 1752 IhiobaciUus Agar I for Acidophilic IhiobaciUus, 1752 IhiobaciUus aiberiis, 1753 Thiobadlha alberlis Agar, 1752 Thiobacillus albcrtis Broth, 1753 'niiohaciilus aquaesulis, 1753, 1754 IhiobaciUus aquaesulis Agar, 1753 Thiobacillus aquaesulis Broth, 1753 IhiobaciUus aquaesulis Medium, 1754 IhiobaciUus Broth 1 lor Acidophilic 'IhiobaciUus, 1754 IhiobaciUus caldus, 1241. 1754, 1755, 1756 IhiobaciUus caldus Agar, 1754 IhiobaciUus caldus Broth. 1755 IhiobaciUus caldus Medium, 1755 IhiobaciUus concretivorus. 1752 'IhiobaciUus cupnnus, 1756 IhiobaciUus cuprinus Medium. 1756 'IhiobaciUus deiicalus, 1579 IhiobaciUus denitrificans, 1059.1526.1683, 1756 IhiobaciUus denitrificans Medium, 1756. 1875 IhiobaciUus/errooxidans, 880, 946, 1241, 1289,1757, 1758 'IhiobaciUus/errooxidans Medium, 1757, 1782 'IhiobaciUus ferrooxidans Medium with Ferrous Sulfate. 1757 'IhiobaciUus ferrooxidans Medium with Tetralhionale, 1757 'IhiobaciUus ferrooxidans Medium with Thiosulfate, 1757 IhiobaciUus ferrooxidans Medium, APH. 1757 IhiobaciUus hahphilus. 1758. 1759. 1768 IhiobaciUus hahphilus Agar. 1758 'IhiobaciUus hahphilus Broth, 1758 'IhiobaciUus hahphilus Medium, 1758 IhiobaciUus Heterotrophic Medium. 1758. 1759 'IhiobaciUus hydrothermalis, 1682 IhiobaciUus intermedins, 1752, 1759.1760 IhiobaciUus intermedins Medium, 1759, 1760 IhiobaciUus Medium, 1760, 1761,1762 IhiobaciUus Medium B. 1762 IhiobaciUus neapolitanus, 1526, 1752, 1762 IhiobaciUus neapolitonus Medium. 1762 IhiobaciUus novellus, 1752, 1763 IhiobaciUus novellus Medium, 1762 IhiobaciUus organoparus, 46, 1759 I hiobacillus perometabolis. 1763 IhiobaciUusperometabolis Medium 1763 IhiobaciUus plumbophilus, 1764 IhiobaciUus plwnbophilus Medium, 1763 IhiobaciUus prosperus, 1764 IhiobaciUus prosperus Medium. 1764

IhiobaciUus species, 1050,1060,1751. 1759. 1760,1761, 1762, 1764,1765, 1785, 1862 Thiobacillus tepidarius, 1764 Thiobacilhts tepidarius Medium, 1764 Thiobacillus thermophilica, 209 Thiobacillus thiooxidans, 1241, 1752, 1754, 1758, 1760,1762, 1764. 1765,1899 Thiobacillus thiooxidans Medium, 1764, 1765.1782. 1875 Thiobacillus thiopanis, 1526, 1579, 1752, 1760.1765.1766.1767 Thiobacillus thioparus Agar, 1765 Thiobacillus thioparus Broth. 1765 Thiobacillus thiopanis II Medium, 1765 Thiobacillus thioparus Medium. 1765 Thiobacillus thioparusii Agar, 1766 Thiobacillus thio/wrusii Broth. 1766 Thiobacilhts th wis iris, 1767. 1768 Thiobacilhts thyasiris Agar. 1767 Thiobacillus thyasiris Broth, 1767 Thiobacillus thyasirisiThiobacillus hahphilus Medium, 1768 Thiobacillus versutus, 1751 ThiobacilluslThermophilus Medium, 1764 Thiobacter subteiraneus, 1195 Thiocapsa halophila, 1455, 1769 Thiocapsa halophila Medium, 1768 Thiocapsa Medium, 1769 Thiocapsa species, 1770 Thiocyanante utilization, 1770 Thiocyanate Agar, 1770 Thiocyanate utilization. 1770 Thiocyanate Utilization Medium, 1770 Thiocystis gelatinosa. 1377 Thiocystis violacea, 1377 Thiofaba Medium,, 1770 Thiofaba species, 1771 Thiogel® Medium. 1771 Thioglycolatc Bile Broth, 1771 Thioglycolatc Broth USP, Alternative. 1772 Thioglycolale Gelatin Medium, 1772 Thioglycolatc HiVcg Agar. 1772 Iliioglycolate HiVeg Medium without Indicator. 1772 Iliioglycolate Medium. 1772 Thioglycolatc Medium with 20% Bile. 1772 Iliioglycolate Medium with Vitamin Kl and

Hernia, 1773 Iliioglycolate Medium without Glucose, 1774 Thioglycolatc Medium without Glucose and Indicator, 1774 'Iliioglycolate Medium without Indicator. 1774, 1775 Iliioglycolate Medium without Indicatoi with Hemin. 1775 Iliioglycolate Medium without Indicator135C,1775 Iliioglycolate Medium, Brewer, 1772,1773 Thioglycolatc Medium. Brewer Modified, 1773

lluoglycolate Medium, Fnrichcd. 1773 Iliioglycolate Medium, Fluid, 1774 lluoglycolate Medium, Supplemented, 1775 Iliioglycolate Medium, USP, 1775 'Iliioglycolate Peptone Glucose Yeast Kxtracl Medium, 1804 'lluoglycolate Potato Liver Medium, 1804 Thiohahmonas denitrificans. 1776, 1778 Thiohahmonas nitratireducens. 1779 Thiohahphilus Medium, 1776, 1777, 1778 Thiohahphilus thiocyanatoxydans, 1776 •lliiol Broth. 1776 Thiol HiVeg Broth, 1779 'lliiol HiVcg Medium. 1779 lliiol Medium, 1779 Thiomicrospira denitrificans. 1780, 1781 Thiomicrospira denitrificatw Agar, 1779 Thiomicrospira denitrificans Broth. 1780 Thiomicrospira denitrificans Medium, 1781 Thiomicrospira halophila. 1776.1777 Thiomicrospira Medium. 1781 Thiomicrospira pelophila. 1782 Thiomicrospira pelophila Medium. 1782 Thiomicrospira species, 1781 Thiomicrospira thermophila, 1193 Thiomicrospira thyasirae. 1768 Thiomonas delicata. 1782 Thiomonas delicata Medium,, 1782 Thiomonas perometabolis. 1783 Thiophaeococcus mangrovi, 1783 Thiophaeococcus mangrovi Medium, 1783 'Iliiophene, 2 Caiboxylic Acid I lydrazide Medium. 1688 Thiorhodococcus bheemlicus. 1496 Thiorhodococcus Medium. 1783 Thiorhodococcus minor, 1784 Thiosphaera Agar. 1784 Thiosphaera Broth. 1784 Thiosphaera pantotropha, 1784, 1785 Thiosphaera pantotropha Medium, 1785 lliiosulfate Citrate Bile Salt Sucrose Agar, 1687 Thiosulfate Salts Broth. 1785 Iluosulfate-Oxidizing Medium. 1785 Thiothrix Agar, 1786 Thiothrix Medium. 1786 Thiothrix nivea, 1786 Thiothrix species. 208.665.971.1227.1243. 1244. 1245, 1246, 1256 ITiiotone™ F peptone, 3 lliome Medium, Modified. 1787 Thraustochytrium sj>ecies, 287 Thraustochylnitm striatum. 1555, 1557 Throat, 1283.1836 lliroat swabs, 1397 lliymidine Auxotroph XPS Medium, 1920 Tibi Medium. 1787 Tick-derived Mycoplasma, 1595 Ticks. 1061 Tteghemiomyces Medium, 1787,1803 Tieghemiomyces pamsiticus, 1787 Tilletia caries, 1192

Index

Tindallia magadiensis. 1787 Tindallia Medium, 1787 TindaUia species, 1788 Tinsdale Agar, 1788 Tinsdale HiVeg Agar Base with Tinsdale Supplement, 1788 Tissierella creatiiiini, 92 Tissierella creatinophila, 1789 Tissierella creatinophila Medium. 1789 Tissierella praeacuta, 1461 Tissue culture. 336.621. 1301. 1789. 1790. 1791, 1792, 1793, 1794. 1795, 1923 Tissue Culture Amino Acids, HeEa, 100X, 1789 Tissue Culture Amino Acids, Minimal I!agle, 50X, 1789 Tissue culture cells, 288. 1791 Tissue Culture Dulbccco Solution. 1790 Tissue Culture Bark Solution. 1790 Tissue Culture Hanks Solution. 1790 Tissue Culture Medium, 199, 1790 Tissue Culture Medium Eagle with Bade Balanced Salt Solution, 1791 Tissue Culture Medium Eagle with Hanks Balanced Salt Solution, 1791 Tissue Culture Medium Eagle, I lei .a, 1792 Tissue Culture Medium Ham I TO, 1792 Tissue Culture Medium NCTC, 109. 1793 Tissue Culture Medium RPMI No. 1640. 1793 Tissue Culture Minimal Medium luigle, 1794 'Tissue Culture Minimal Medium Eagle Spinner Modified, 179S Tissue Culture Minimal Medium Eagle with Marie Balanced Salts Solution, 1794 Tissue Culture Tyrode Solution. 1795 Tissue Culture Vitamins Minimal I-agle, 100X. 1795 IMA Mineral Medium, 1795 TMAO HiVeg Medium. 1796 TMAO Medium. 1818 TMBS4 Medium, 1796. 1797 T-mycoplasmas, 1287 TN Broth. 1823 TN HiVeg Agar, 1799 TNSAAgar. 1820 TNT Medium. 1799 TOC Agar, 1799 Todd-Hewitt Broth, 1799 Todd-Hewitt Broth, Modified, 1800 Todd-Hewitt HiVeg Broth, 1800 Todd-Hewitt Medium, 1800 Toiletries. 810 Tokophrya infusionum, 158 Tokophrya lemnarum. 1339 Toluene utilization, 1183 Toluidinc Blue DNA Agar. 1800 Tolypothrix tenuis, 214 Tomato Dextrin Yeast Medium. 1800 Tomato Juice Agar, 1800,1801 Tomato Juice Agar Special, 1801 Tomato Juice Broth, 1801

Tomato Juice HiVeg Agar, 1801 Tomato Juice HiVeg Agar, Special, 1801 Tomato Juice I liVcg Medium Base with Tomato Juice and Cycloheximide, 1802 Tomato Juice Medium. 1802 Tomato Juice Milk Agar, 1802 Tomato Juice Yeast Extract Medium. 1802 Tomato Juice Yeast Hxlracl Milk Medium, 1802 Tomato Paste Oatmeal Agar, 1803 Tonsillophilus suis. 1255, 1890 Top Agarose. 1803 Topical drugs, 1683,1684 Torpedospora radiaia. 287 Torula denmtia, 1004 Tortdaspora delbrueckii, 1000 Torulopsis Medium, 1803 Toxin-producing Escherichia colt. 1671 Toxins, 1471 Toxoplasma gondii. 1803 Toxoplasma Medium. 1803 TPBY. 1840 TPEY Agar, 1803 TPBY HiVeg Agar Base with Egg Yolk, Tellurite, and Polymyxin B, 1804 TPGY Broth. 1823 IPCY Medium, 1804 TPGYT Broth. 1824 TPE Medium, 1804 TPT, 18 Medium,, 1805 TPY Medium. 1805 TPYG Medium. 1805 Trace Element Mixture, 5 Trace Element Solution HOLE. 5, 1805 Trace Element Solution SE-8. 5 Trace Elements Solution SI.-10, 5 Trace Elements Solution SE-6, 5 Trace Elements Solution SE-7,5 Trace metals, 1805 Trace Metals A-5 Mix, 5 Trademarks. 8 Transgrow Medium, 1805. 1806 Transgrow Medium with Trimethoprim. 1806 Transgrow Medium without Trimethoprim, 1807 Transport, 89, 90, 1485 Transport Medium. 297.309.319.958.1287. 1626, 1650, 1651. 1653, 1654. 1806, 1807.1888, 1901 Transport Medium Stuart, 1807 Treatment plants, 1427 Trebouxia Agar, 1807 Treboiixia magna, 1808 Trehalose fermentation, 1392 Tremella mesenterica, 392 Trepomonas agilis. 831. 1859 'Treponema brennahorense, 373 Treponema bryantii. 616. 1488. 1808 Treponema bryantit Medium, 1808 Treponema deniicola. 1302, 1329. 1809. 1812

2029

Treponema denfieola Medium, 1808 Treponema hyodysenteriae, 1852 Treponema Isolation Medium. 1809 'Treponema macrodentitun, 1810 Treponema macrodentium Medium, 1810 Treponema Medium, 1810, 1811, 1812 Treponema Medium 1, 1812 Treponema Medium 2, 1813 Treponema Medium 3. 1813 Treponema Medium, Prereduced, 1813 Treponema oralis, 1329 7 ix'ponema pallidum, 1812 Treponema pectinovorum, 1063 'Treponema phagedenis, 253, 1454, 1609 Treponema sacciuirophilum, 1256, 1814 Treponema saccharophilum Medium, 1813 Treponema socranskii, 1302, 1332 Treponema species, 617. 659, 1302. 1812 Treponema succinifaciens, 616, 1814 Treponema succinifaciens Medium. 1814 Treponema vincenlii, 1302 Treponcmes, 1396, 1450.1460, 1812. 1813 Triangularia halislae, 944 Tribonema aequale. 70 Tributyrm Agar, 1814 Tnbulyrin I HVcg Agar Base with Tributyrin. 1815 Trichamoeba species, 1589 Trichlorophenol Medium, 1815 Trichococcus Jlocculifonnis, 1815 Trichococcus Medium, 1815 Trichoderma longibrachiatum, 11 Trichoderma pseudokoningii. 1004 Tricholoma hakamatsulake, 472 Tricholoma equestre, 1325 Tricholoma Jlamvirens, 1199 7richolomafulvocastaneum, 472 Tricholoma matsutake, All Tricholoma ponderosum. All Tricholoma species, 1199 Trichomitus batrachorum, 1861 Trichomonas gallinae, 467, 1861 Trichomonas HiVeg Agar Base with Scrum. 1815 Trichomonas HiVeg Agar Base with Senim and Selective Supplement. 1815 Trichomonas HiVeg Broth Base. Kupfcrbcrg. 910 Trichomonas Medium. 1816 Trichomonas Medium No. 2. 1816 Trichomonas Modified CPI.M HiVeg Medium Base. 1202 Trichomonas Selective Medium, 1816 Trichomonas species, 592, 909,910, 1818. 1861 Trichomonas tenax, 1861 Trichomonas vaginalis. 467,931.1202, 1203, 1815,1816,1861, 1862 Trichomycete species, 930 Trichophaea abundans, 988 Trichophaea contradicta, 988 Trichophyton Agar 1, 1816

2030

Index

Trichophyton Agar 2. 1817 Trichophyton Agar 3, 1817 Trichophyton Agar 4, 1817 Trichophyton Agar 5, 1817 Trichophyton Agar 6, 1817 Trichophyton Agar 7, 1817 Trichophyton IliVeg Agar 1. 1818 Trichophyton IliVeg Agar 2. 1818 Trichophyton HiVcg Agar. 3. 1818 Trichophyton I liVcg Agar 4. 1818 trichophyton IliVeg Agar 5. 1818 Trichophyton mentagrophytes. 1529. 1532 Trichophyton ruhrum, 1532 Trichophyton schoenleinii, 265 Trichophyton species, 1575, 1817. 1818 Trichophyton verrucositm, 265, 1396 Trichophyton violaceum, 449 Trichosei™ Broth, Modified, 1818 Trichosphacrium species. 614. 822 Trichosporon nigrescens, 1001 Trichospartm species, 1941, 1942 Trichotomospora caesia, 1939 Trichurus spiralis, 1410 Trigonopsis variabilis, 1 (XX) Trimethylamme jV-Oxide Medium, 1818 Trimethylamine jV-Oxidc IliVeg Medium, 1796 Trimyema shoalsia, 1556 Triple Sugar Iron Agar, 1819 Triple Sugar Iron Agar, IliVeg, 1819 Tripospcrmtnn myrli. 944 Tris Yeast Fxtract Pqrtonc Agar. 1820 Iris Yeast Fxtract Peptone Broth, 1820 Tris YP Agar, 1820 Tris YP Broth, 1820 Tritrichomonas augusta. 1861 Tritrichomonas foetus, 467, 1861, 1862 Tnlnchomonas mohilensis, 467 Tritrichomonas suis, 1861, 1862 Trichophyton mentagrophytes, 265 Trychophylon nihruin, 265 Trypallavin Nalidixic Acid Scrum Agar. 1820 Trypanopiasmu borreli. 958 Tiypanosoma avium, 600 Trypanosoma hennetti, 600 Trypanosoma cen'i, 600 'Trypanosoma chat ton i. 600 Tiypanosoma conoirhini, 249, 600 Trypanosoma crttzi, 600, 958, 1300 Trypanosoma cyclops, 600 Trypanosoma failisi, 600 Tiypanosoma gamhiense, 1820 Trypanosoma lewisi, 600 Trypanosoma lucknowi. 600 Tiypanosoma mega, 600 Tiypanosoma musculi, 600 Tiypanosoma ncveulemairei. 600. 912 Tiypanosoma ranaiwn, 600 Tryifanosoma rangeli, 600 'Tiypanosoma rhodesiense, 1820

Trypanosoma rotaforium. 600 Trypanosoma species, 230, 912 Trypanosoma lamiasi, 600 Trypanosoma theileri, 1229 Trypanosome Medium, 1820 Tryplic Digest Broth, 1820 Tryplic Nitrale Medium. 1821 Tryplic Soy Agar. 1825 I ryptic Soy Agar with 0.6% Yeast lixtract, 1821 Tryplic Soy Agar with Magnesium Ions. 107, 1821 Tryptic Soy Agar with Magnesium Sulfate. 1821 Tryplic Soy Agar with Magnesium Sulfate and Sodium Chloride, 1821 Tryplic Soy Agar wilh Sodium Chloride, 1821, 1822 Tryplic Soy Blood Agar, 1827, 1835 Tryptic Soy Blood Agar with VAN. 4.1821 Tryplic Soy Broth. 1822 Tryptic Soy Broth with 0.001 A/ Calcium Chloride, 1822 Tryptic Soy Broth with 0.1 % Potassium Nitrale, 1822 Tryptic Soy Broth with IA/KC1. 1822 "Tryptic Soy Broth with Petrous Sulfate. 1831 Tryplic Soy Broth with Magnesium Ions, 1682, 1822 Tryptic Soy Fast Green Agar. 1822 Tiypticase™, 1% Solution, 1847 Trypiicase™ Agar Base, 1822 TrypticaseIM Agar Base with Carbohydrate. 1813 Trypticasc™ Azolcctin Tween Broth Base, 1683 Trypiicase™ Broth. Supplemented. 1823 Trypiicase™ Glucose Fxtract Agar, 1823 Trypticasc™ Novobiocin Broth. 1823 Trvpiicase™ Peptone Glucose Yeast Extract ' Broth. 1823 Trypticase™ Peptone Glucose Yeast Fxtract Brolh with Trypsin. 1824 TrypticaseIM Peptone Glucose Yeast Fxtract Broth, Buffered, 1823 Trypticasc™ Phytone Medium, 1824 Trypticase™ Soy Agar. 1825 Trypticase ,M Soy Agar with 1% Glucose. 1825 Trypticasc1M Soy Agar with Cefazidimc. 1825 Trypticasc1M Soy Agar with Glycerol. 1825 Trypticase™ Soy Agar with I luman Blood, 1826 Trypticase™ Soy Agar with Lecithin and Polysorbate, 80, 1826 Trypticasc™ Soy Agar with Sheep Blood, 1827 Trypticasc™ Soy Agar with Sheep Blood and Gentamicin. 1827 Trypticasc™ Soy Agar with Sheep Blood, Formate and Fumarate, 1827

Trypiicase™ Soy Agar with Sheep Blood. Sucrose, and Tetracycline, 1828 Trypticasc ,M Soy Agar with Tobramycin, 1829 Trypticasc™ Soy Agar with Yeast Fxtract

and Glucose, 1830 Trypticasc™ Soy Agar. Modified, 1826. 1827 Trypticasc1" Soy Agar, Modified with Horse Scrum. 1827 Trypticasc™ Soy Broth, 1830 Trypticase™ Soy Broth Agar Modified, 1830 'Trypticasc™ Soy Broth wilh 0.1% Agar, 1830 Trypiicase™ Soy Broth with 1.5% NaCl, 1834 Trypticasc™ Soy Broth with 10mA/Glucose, ' 1831 Trypticase™ Soy Broth with Calcium Chloride, 1830 Trypticase™ Soy Broth with Cefazidime, 1830 Trypticasc™ Soy Broth with Fetal Calf Serum, 1831 Trypticasc™ Soy Broth with Glycerol. 1831 Trypticase™ Soy Broth with I IOINC Scrum, 1831 Trypticase™ Soy Broth with I luman Blood, 1832 Trypticase™ Soy Broth with Neomycin, 1832 Trypticasc™ Soy Broth with Sodium Chloride and Sodium Pyruvate, 1833 Trypticasc™ Soy Broth with Tobramycin. 1833 Trypticasc™ Soy Broth with Twcen 80.1834 Trypticase™ Soy Broth with Yeast Extract, 1834 Trypticase™ Soy Broth without Glucose, 1834 Trypticasc™ Soy Broth, Modified, 1832 Trypticase™ Soy Glucose Medium, 1834 Trypticase ™ Soy Polymyxin Broth, 1834 Trypticase™ Soy Soil Kxtract. 1852 Trypticase™ Soy Tryptose Broth, 1835 Trypticase™ Soy Yeast Kxtract Agar, 1835 Tiypticase™ Soy Yeast Extract Medium, ' 1853 Trypticasc™ Soy Yeast I'xtract Starch Medium, 1854 Trypticasc1" Starch Agar, 1836 Trypticase™ Sulfite Neomycin Agar, 1853 Trypticasc 1 " Tellurite Agar Base, 1836 'Trypticase™ Yeast Fxlracl Glucose Medium, 1836.1857.1859 'Trypticase™ Glucose Agar, 1823 Trypticasc1" Nitrate Broth. 874 Tiypticase'" jieptone, 3 Trypticasc™ Phylonc™ Glucose Medium. 1824 'Trypticasc'" Phylonc 1 " Glucose Medium. with Twcen™ 80. 1824

Index

Trypticase™ Phytone™ Medium, 1824 Trypticase™ Serum Seawater Agar. 1824. 1825 Trypticase™ Soy Agar with 3% Sodium Chloride. 1829 Trypticase™ Soy Agar with Sheep Blood and Streptomycin. 1828 Trypticase™ Soy Agar witli Sheep Blood and

Twees 80,1828 Trypticase ™ Soy Agar with Sheep Blood and Vancomycin, 1828 Trypticase™ Soy Agar with Sodium Chloride, 1829 Trypticase™ Soy Agar with Sodium Chloride, Horse Serum, and Penicillin, 1829 Trypticase1 M Soy Agar with Starch. 1829 Trypticase™ Soy Agar with Tobramycin, 1829 Trypticase™ Soy Agar with Yeast Extract, 1829 Trypticase™ Soy Agar-Magnesium Sul raleSodium Chloride Agar, 1826 Trypticase™ Soy Broth with 0.1% DL-7I lydroxybutyric Acid, 1832 Trypticase™ Soy Broth with 0.1% Yeast Extract, 1834 Trypticase™ Soy Broth with 1.5% Sodium Chloride, 1833 Trypticase™ Soy Brolh with 10/HA/Glucose. 1831 Trypticase™ Soy Broth with \M Potassium ' Chloride, 1832 Trypticase™ Soy Broth with Cefa/idime, 1830 Trypticase™ Soy Broth with Ccla/idine, 1835 Trypticase™ Soy Broth with Ferrous Sulfate, 1831 Trypticase™ Soy Broth wilh Sea Sails, 1833 Trypticase™ Soy Broth with Sheep Blood. 1833 Trypticase™ Soy Brolh wilh Starch. 1833 Tiypticase™ Soy Broth with Yeast Fxtract, 1834

Trypticase™ Soy Broth. Modified, 1832 Trypticase™ Soy Sheep Blood Agar. 1835 Tryplicasc™ Soy Yeast Extract Agar. 1835 Trypticase™ Soy Yeast I Extract Medium, 803. 1836 Tryptone, 2, 3 'Tryptone Agar, 1836 Tryptone Agar Base. I li Veg. 1836 Tryptone Agar, Hi Veg, 1837 Tryptone Beef Yeast Extract Acetate Agar, 1686 Tryptone Bile Agar. 1837 Tryptone Bile Glucuronide Agar. Harlequin, 1837 Tryptone Bile X-glucuronidc Agar. 1686 Tryptone Bile X-glucuronide Agar. Chromocult, 1837

Tryptone Broth. 1798,1837, 1838. 1845 Tryptone Broth wilh CaC12, 1838 Tryptone Broth, 1%, 1838 Tryptone Broth, Hi Veg. 1838.1845 Tryptone Glucose Beef Fxtract Agar. 1838 Tryptone Glucose Beef Fxtract Agar with Sucrose, 1838 Tryptone Glucose Beef Fxtract Agar with Ycasi Extract. 1838 Tryptone Glucose Fxtract Agar, 1838 Tryptone Glucose Extract Hi Veg Agar. 1838 Tryptone Glucose I liVeg Agar, 1839 Tryptone Glucose Medium. 1839 Tryptone Glucose Yeast Agar, 1402, 1631 Tryptone Glucose Yeasi Broth, 1631 Tryptone (ilucose Yeast Extract I liVeg Agar, 1838 Tryptone Glucose Yeast Extract HiVeg Broth, 1839 Tryptone Glucose Yeast Plxtract Medium. 1704 Tryptone Glucose Yeast Extract Methionine Medium, 1704 Tryptone in Sea Water Agar. 1841 Tryptone Lactose Iron Hi Veg Agar, 1839 Tryptone NaCl Tbiamme Medium. 1799 Tryptone Nitrate HiVeg Medium, 874, 1840 Tryptone Peptone Glucose Yeast Extract I li Veg Broth Base without Trypsin, 1840 Tryptone Phosphate Brain Heart Infusion Yeast Extract Agar. 1840

Tryptone Phosphate Broth, 1840 Tryptone Phosphate HiVeg Broth. 1840 Tryptone Salt Agar, 1798 Tryptone Salt Broth, 1798.1799 Tryptone Soy Agar, 1841 Tryptone Soy Agar pH 5.5, 1841 Tryptone Soy Agar pi I 6.5, 1841 Tryptone Soy Agar with 1 % Sodium Chloride, 1842 Tryptone Soy Agar with 4.5% Sodium Chloride, 1842 Tryptone Soy Agar with ItorseBlood. 1841 Tryptone Soy Agar with Sheep Blood. 1842 Tryptone Soy Agar, Hi Veg, 1841 Tryptone Soy Broth. 1842 Tryptone Soy Broth wilh 0.1% Tween™ 80. 1842 'Tryptone Soy Brolh with Yeast Extract, 1842 Tryptone Soy Broth. I li Veg. 1592,1842 Tryptone Soy HiVeg Agar with Added Sodium Chloride and Cocarboxylasc. 1843 'Tryptone Soy I IiVcg Agar with Lecithin and Polysorbate. 80, 1843 'Tryptone Soy HiVeg Agar with Magnesium Sulfate, 1843 Tryptone Soy HiVeg Broth with 0.1% Agar. 1593,1843 Tryptone Soy I li Veg Broth with 10% Sodium Chloride and, 1% Sodium l*yruvate. 1843 Tryptone Soy HiVeg Broth without Glucose, 1593

2031

Tryptone Soy Salt I li Veg Agar with Magnesium Sulfate, 1844 Tryptone Soy Yeast Extract HiVeg Agar, 1844 Tryptone Soy Yeast Extract Hi Veg Broth. 1844 Tryptone Soya Agar. HiVeg. 322. 1592 Tryptone Soya I liVeg Agar with Lecithin and Tween 80, 1156 Tryptone SoylliVcg Broth without Glucose, 1843 Tryptone T, 3 Tryptone 'Thioglycolate Medium, 1844 Tryptone Water Broth. 1845 Tryptone Water Broth, 1 li Veg, 1845 Tryptone Water. Hi Veg. 1838, 1845 Tryptone with Sodium Chloride Broth. 1839, 1845 Tryptone Yeast Extract Agar, 1845 Tryptone Yeast Extract Agar, 1. 1845 Tryptone Yeast Extract Broth, 885 Tryptone Yeast Extract Glucose Medium, 1859 Tryptone Yeast Extract Glucose Salt Medium. 1860 Tryptone Yeast Fxtract HiVeg Agar with Carbohydrate. 1846 Tryptone Yeast Extract I liVeg Broth. 884 Tryptone Yeast Extract Medium. 1846 Tryptone Yeast Plxtract Mineral Medium. 1846 Tryptone Yeast Extract Salt Medium. 1846. 1858 Tryptophan, 1% Solution, 1847 Tryptophan assay, 1847 Tryptophan Assay Medium. 1846 Tryptophan Broth, 1847 Tryptophan Hi Veg Medium. 1847

Tryptophan test, 874 Tryptosc. 2, 3 Tryptose Agar, 1847 Tryptosc Agar with Citrate. 1847 Tryptose Agar with Sheep Blood, 1848 Tryptose Agar with Thiamine, 1848 Tryptose Agar with Thiamine IIC1,1 liVeg, 1848 Tryptose Agar, HiVeg, 1848 Tryptose Blood Agar. 1848 Tryptose Blood Agar Base, 1849 Tryptose Blood Agar Base wilh Yeast Extract, 1849 Tryptose Blood Agar Base with Yeast Fxtract. Hi Veg. 1849 Tryptose Blood Agar Base, HiVeg with Sheep Blood. ^849 Tryptose Brolh, 1849 Tryptosc Brolh wilh Citrate, 1849 Tryptose Broth, HiVeg, 1850 Tryptose Cycloserine Dextrose Agar, 1850 Tryptose Cycloserine Glucose HiVeg Agar Base with Cycloserine. 1850 Tryptose Phosphate Agar, 1850

2032

Index

Tryptose Phosphate Broth, 1850 Tryptosc Phosphate Broth. HiVeg, 1850 Tryptose Phosphate Broth, Modified, 1851 Tryptose Sulfite Cycloserine Agar, 1851 Tryptose Sulfite Cycloserine Agar with Polymyxin and Kanamycin, 1851 Tryptose Sulfite Cycloserine Agar without Egg Yolk, 1852 Tryptosc Sulfite Cycloserine Agar, Fluorocult. 1853 TSAgar, 1852 IS Medium TorSpirochaeta caldaria. 1852 TS Soil Extract, 1852 ISA,5400Selective Isolation Medium, 1852 ISA Blood Agar, 1827, 1835 ISA II1M, 1827 TSA II™ with Sheep Blood and Genlamicin. 1827 TSA NaCl. 1829 ISAM HiVeg, 1843 TSAMS, 1826 TSAYE, 1829 TSBY Salt Medium. 181.1853 TSBYE, 1834 TSC Agar, 1851 TSC Agar without Egg Yolk, 1852 TSC Agar. Fluorocult. 1853 TSFA, 1822 TSIAgar. 1819 TSNAgar. 1853 T-strain mycoplasmas, 1865, 1866 Tsukamurella paurometaltolum. 454. 754. 967.968,1330.1470 TSY Medium, 1853 TSYES Medium. 1854 IT Broth. 1699 TT Broth, Hajna. 1700 IT Broth, USA, 1700 IT HiVeg Broth Base, 1701 TTC Agar. 1702 TTC HiVeg Broth Base, 887 TTD Medium, 1854 TTYSH Medium. 1854 Tube dilution method. 391 Tuber alhidum, 775 Tuber ntj'um, 775 Tuberculiua maxima. 318 Tuberculiua pcrsicina. 318 Tuberculosis, 1164, 1165 Turbid specimens. 645, 1772 Tween™ 80 Agar. 1407 Twccn™ 80 Hydrolysis Broth. 1856

Tween™ 80 Hydrolysis Medium, 1856 Tween™ 80 hydroly/ation, 1856 Tween™ 80 Oxgall CalTeic acid Agar. 1799 Twccn™ 80A Medium. 1855

Tween™ 80-Agar, 1855 Tween™ 80A Agar, 1855 Twccn™ 80A Broth. 1855 IT Medium. 1856 TY Medium, 1856

TY Medium, 2X. 1856 TY Salt Medium, 1857 TY Salts Medium, 1857 TYE Broth Medium, 1857 TYEHES Medium. 1857 IYE-CO, 420 TYEG Medium. 1857 TYES Medium, 1858 TYG Medium. 1858,1859 TYGM-9 Medium, 1859 TYGPN Medium. 1859 TYCiS Medium. 1860 TYI-S-33 Medium, 1860 TYM Basal Medium. Modified 1, 1861 TYM Basal Medium, Modified 2, 1861 TYM Basal Medium. Modified 3.1861.1862 TYM Basal Medium, Modified 4, 1862 TYM Medium. 1862 TYN Medium, 1862 Tyndalli/ation, 9 Tyrosine Agar. 1862 Tyrosine Casein Nitrate Medium, 1863 Tyrosine utilization. 1862 TYX Medium, 1863

TZC Selective Medium, 1863 I U Agar Plates, 1864 U Broth, 1864 U4 Medium, 1866 U9 Broth. 1865 U9 Broth with Amphotericin B, 1865 U9B Broth. 1865 U9C Broth, 1866 UB HiVeg Agar, 1867 UBA Medium. 1867 lllochulium a/rum, 1410 Ulocladium bonylis. 1410 Ulocladium charlarum, 1410 Ulocladium curcurbitae, 1410 Ulothrix gigas, 70 Unclassified bacterium DSM 12783. 103 Unclassified DSM bacterium, 19 Universal Beer Agar, 1867 Universal Beer HiVeg Agar with Beer. 1867 Universal Medium For Yeasts, 1868 Universal Precnrichment Broth. 1868 University of Verment 1 listeria Primaiy Selective Enrichment Broth. 953 University of Vermont II Listeria Primary Selective enrichment Broth, 954 University of Vermont Listeria luirichment Broth. 1876 University of Vermont Modified Listeria Enrichment Broth. 1876 Urea Agar. 1868. 1869 Urea Agar Base, 1869 Urea Agar Base. Christensen, 1869 Urea Broth. 1869,1872 Urea Broth 10B for Ureaplasma urealyticum, 1869 Urea Broth Base, 1870

Urea HiVeg Agar Base Autoclavablc with Urea, 1870 Urea Nutrient Agar. 1870 Urea R Broth, 1871 Urea Rapid Broth. 1871 Urea Semisolid Medium, 1871 Urea Test Broth. 1871 Urea utilization. 878 Ureaplasma Agar Plates, 1864 Ureaplasma Broth, 1864 Ureaplasma diversum, 1867 Ureaplasma Medium, 1872 Ureaplasma species, 12, 13. 14, 15, 66, 171, 267. 1592, 1864.1870. 1872 Ureaplasma urealyticum, 12, 13, 14, 15,66, 171. 597, 1630.1865. 1866. 1870. 1874 Urease, 1870 Urease. 1409.1410.1865.1866.1869. 1870. 1871 Urease Color Test Medium, 1865 Urease production. 375.1646. 1690, 1869. 1870, 1871,1872 Urease Test Agar. 1869 Urease Test Broth, 1872 Urethral specimens, 821 Uric acid, 1872, 1873. 1874 Uric Acid Agar. 1872,1873 Uric Acid Agar for Clostridia, 1873 Uric Acid Broth, 1873 Uric Acid Broth for Clostridia. 1873 Uric Acid Medium. 1873 Uric Acid Semisolid Agar for Clostridia. 1874 Uric Acid Utilization Agar, 1874 Urinary. 1450 Urinary pathogens, 392 Urinary tract. 260 Urinary tract infections, 386, 845 Urine, 12,13,14,15, 66,133,264,392,992. 1400. 1695,1700, 1701 Urogenital. 1287 Urogenital Mycoplasma Broth Base, 1874 Uses of Media, 9 Usneajlorida. 951 USP Alternative Thioglycolate Medium, 1638 Ustilago Complete Agar II, 1874 Ustilago Complete Broth II. 1875 Ustilago Medium, 1875, 1876 Ustilago Minimal Medium. 1876 Ustilago species, 1875, 1876 IJTI Agar, Modified HiCrome™, 845 UVM. 955 UVM listeria Enrichment Broth, 1876 UVM Modified Listeria Enrichment Broth. 1876 V

V Agar. 1877 V C A Inhibitor, 7 V C A T Inhibitor, 7 V C N Inhibitor, 7

Index

V C N 1 Inhibitor, 7 V24N Medium, 1878 V-8™ Juice Sea Water Agar, 1878 V-8™ Rye Agar, 1878 V-8 1M Agar. 1877 V-8™ Agar, Half-strength, 1877 V-8™ Agar. pH 7.2. 1877 V-8™ Juice Agar, 1878 V-8-0 Agar. 1877 VA Vitamin Solution, 5 Vaccine production. 753. 1540 Vaccines, 345,445 Vagina, 1836 Vaginal, 1515,1516, 1517 Vaginal specimens, 821 Vaginitis, 719 Vagococcui fluvial*, 984, 1429, 1933. 1945 Vagococcus salmoninarwn, 1429, 1945 Vahlkampjia avora, 1217 Vahlkampfia lobospinosa, 1454 Vampirovibrio chlorellavorus, 1039 Van Niel's Yeast Agar. 1879 Van Niel's Yeast Agar with 25% Sodium Chloride, 1879 Van Niel's Agar, 1878 Van Niel's Medium. Modified, 1879 Van Niel's Yeast Agar with Glutamate. 1879 Van Niel's Yeast Agar with Sodium Chloride, 1879 Van Niel's Yeast Agar with Succinate, 1879 Van Niel's Yeast Medium with Pyruvate. Modified, 1879 Vancomycin resistant cnterococci. 1895 Vancomycin resistant Enterococcus, 378 Vanillatc Medium, 1880 Vannella miroides, 707 Vannella species. 1011 VCR Medium, 1880 Veal Infusion Agar, 1880. 1881 Veal Infusion Broth, 1881 Veal Infusion Broth with Horse Serum, 1881 Veal Infusion Brom with Rabbit Serum, 1881 Veal Yeast I-xtract Medium, 1898 Veillonella Agar, 1882 Veillcmella HiVeg Agar Base with Lactate, 1882 Veillonella HiVeg Agar Base wiUi Lactate and Vancomycin, 1882 Veillonella Medium, 1882 Veillonella Medium. DSM 1882 Veillonella parvula. 1419.1882 Veillonella Selective Medium, 1882 Veillonella species, 1485, 1882, 1883 VENCHI2 Medium. 1883 Venenivibrio stagnispumanfis. 1884 Venenivibrio stagnispumantis Medium, 1883 Vero tissue culture, 336 Verotoxin-producing, 1823 Verticiliiumfungicola, 1348 Verticil! ium later it mm 761 Viability-Preserving Microbiostatic Medium. 1892

Vibrio adapsatus, 1554 Vibrio Agar, 1884 Vibrio alginolyitcus. 378 Vibrio alginolvticus, 363, 733, 799, 1310, 1829.1914 Vibrio angiallarmn. 280 Vibrio bivalvii, 1310 Vibrio camphellii, 1554 Vibrio cholera. 221,1923 Vibrio cholerae, 66, 75, 76,321, 363, 378, 445.458.811,812.814.851.1222.1308. 1654, 1687. 1798. 1799, 1838. 1884 Vibrio cholerae, 221 Vibrio costicola. 229, 1554.1884 Vibrio costicola Medium, 1884 Vibrio diazotrophicus. 1884 Vibrio fischeri. 1394, 1395. 1555 Vibriofluvialis, 1617 Vibrio fiirnissii, 1617 Vibrio harveyi. 1009, 1395. 1554, 1558 Vibrio hemolysin, 1899 Vibrio HiVeg Agar, 1884 Vibrio hallisae, 1617 Vibrio iiquefaciens, 1667 Vibrio mediterranei, 1310, 1554 Vibrio Medium, 1884 Vibrio mimiciK, 363, 1923 Vibrio natriegens. 1310, 1554. 1885 Vibrio natriegenx Medium, 1884 Vibrio ordalii. 1015. 1617 Vibrio orien talis, 1310 Vibrio parahaemolytiais, 253.378,363,799, 1310, 1654, 1687. 1885 Vibrio parahaemolytiais Agar, 1885 Vibrio parahuemolyticus Sucrose Agar. 1885 Vibrio parahaemolytiais Sucrose HiVeg Agar, 1885 Vibrio pelagigins, 1310 Vibrio proteolyticus. 799 Vibrio salmonicida, 1554, 1930 Vibrio species, 66. 70, 75. 76,117, 146, 216. 320, 336, 337,458. 485,486, 733, 747, 807.853.971.1006.1310,1311.1335. 1336, 1395, 1436. 1537, 1538, 1554, 1558, 1585, 1654, 1798, 1799, 1821, 1826.1885 Vibrio vallismortis, 1885 Vibrio vallismortis Medium, 1885 Vibrio vulnificus, 1310, 1553, 1617, 1885. 1886 Vibrio vulnificus Agar. 1885 Violet Peptone Bile Lactose Broth. 1886 Violet Red Bile Agar, 1886 Violet Red Bile Agar with MUG. 1886 Violet Red Bile Agar, HiVeg, 1886 Violet Red Bile Glucose Agar, 1887 Violet Red Bile lactose MUG Agar. 1894 Violet Red Glucose HiVeg Agar with Lactose. 1887 Violet Red Glucose 1 liVeg Agar without Lactose. 1887 Violet Red HiVeg Agar. 1887

2033

Violet Red 1 liVeg Agar (1.2%), 1887 Violet Red HiVeg Broth, 1887 Viral Transport Medium, 1887 Viridans enterococci, 1191 Viridans streptococci. 1191 Viscous specimens, 645, 1772 Vitamin B ) 2 assay. 172 Vitamin B 12 assay, 172 Vitamin B| 2 Assay Medium. 1888 Vitamin B, 2 Assay Medium with Colistin, 1889 Vitamin B 12 assay testing. 1889 Vitamin B, 2 HiVeg Agar, 1889 Vitamin B 12 Medium, 1889 Vitamin B, 2 Nutrient Agar. 1890 Vitamin B 6 Blood Agar. 1888 Vitamin B 6 Blood Agar with Pyridoxal-HCI. 1888 Vitamin free casamino acids, 4 Vitamin K| Solution. 5 Vitamin Medium for Sficrohacterium, 1890 Vitamins, 920 Vitox Supplement, 5 Vitreochlamys incisa, 70 Vttreoscilla species, 208 Vitreoscilia stercoraria, 319 Vittreoscilla stercoraria. 761 VL Agar with Blood. 1890 VL Medium, 1890 VM Medium, 1891 VM1 Medium. 1891 VMGII Medium, 1892 Vogel and Johnson Agar. 1892 Vogel N Medium, 1272 Vogel S Medium for Slime-Like Neurospora. 1893 Vogcl-Bonncr Minimal Medium. 1892 Vogel-Johnson Agar Base, HiVeg. 1892 Voges- Proskauer lest, 888 Voges-Proskauer Medium, 1894 Voges-Proskauer reaction, 1236, 1237 Volcaniclla curihalina. 1154 Von Hofslen & Malmcpiist Medium B. 1893 VPAgar, 1885 VP Broth, Modified, Smith, Gordon, and Clark. 1893 VP I liVeg Medium, 1893 VP Medium. 1893 VPS A, 1885 VRBAgar. 1886 VRB MUG Agar. 1894 VRI-:, 378, 1895 VMS Agar, 1894, 1895 VRI- Broth. 1895 VIM, 1888 \ ulcanihacilltm Medium, 1895, 1896 Vulcanibacillus modesticaldus. 1896 Vulcanithermus mediatlanlicus, 1898 Vulcanithermus Medium. 1897 VVA, 1885 VY Agar, 1898 VY Medium, 1898

2034

Index

VY/4-SWS Agar. 1898 VY2 Agar, 1898 VYSAgar, 1898 W

W Medium, 1899 Wadowsky-Yee Medium, Modified, 1256 Wadowsky-Yee Medium. 204 Wagatsuma Agar. 1899 Wagalsuma HiVeg Agar Base with Red Blood Cells, 1899 Wakimoto Medium, Modified, 1899 Waksman's Glucose Agar, 1899 Waksman's Sulfur Medium, 1900 Wall Defective Bacterial Medium. 1900 Wallemia sebi, 988, 1332 Wallcnstcin Medium. 1900 Wallerstein Laboratory Differential Agar, 1910 Wallerstein Laboratory Differential Medium. 1910,1911 Wallerstein Laboratory Medium with Tomato Juice, 1911 Wallcrstcin Laboratory Nutrient Agar. 1911 Wallerstein Laboratory Nuuient Broth, 1912 Walsby Medium. 1900 Wang's Semisolid HiVeg Medium with Blood, 1900 Wang's Transport Storage Medium, 1901 Wardomyx-es dimerus, 988 Wardoimx.es simplex. 1476 Wastewater, 11,156,201,207,261,263, 266,916.991, 1031,1032 Water, 20, 58, 162, 163,201, 203,204, 206, 207, 261. 262. 263.266. 281.290.291. 292,439.615, 623.641,642.648,649. 650, 655, 657, 658, 709, 732,857, 932, 933,991.992,993.1005.1026. 1031. 1032, 1053, 1066, 1067, 1069, 1156, 1157.1167.1168,1186.1196. 1227. 1228,1242, 1243, 1244, 1245, 1246, 1252,1253, 1256,1303, 1314, 1318, 1338.1403.1427.1440.1514. 1515. 1567,1631, 1636.1682,1690. 1823, 1825,1830, 1943 Water Agar. 1901 Water samples, 156,170, 1327 Water-Blue Mctachromc-Yellow Lactose Agar, 719 Waters. 158. 330.669.670, 758. 902. 1150. 1463,1472, 1473.1648.1938 Waxy Mai/e Starch Medium, 1901 Wayne Sulfatasc Agar. 150 WCX Agar, 1901 Web Resources, 10 Weeksella v/rava, 1304 Weeksella zoohelcum, 1304 Weisselia thailandensis, 1232 Wcit/man. Silva-Hutncr Agar, 1901 Wesley Broth, 1902 Wesley Broth Base with Antibiotics and Hematin, 1902

Wesley 1 liVeg Broth Base with Antibiotics and Hematin. 1902 Wheat Peptone Agar. 1903 Whey Agar, 1903 Whey Broth. 1903 Wicket ham BfOth, 1903, 1904 Wickcrham Broth with Raffinose. 1904 Wickerham Carlxm Base Broth, 1927 Wickerhamiella domercqiae. 1935 Wilbrinck Agar for Xanthonwiuts, 1904 Wilbrinck Agar for Xanthomonas albilineans, 1904 Wild yeasts. 977 W:lkiiiN Chalgren Agar. 1905 Wilkins-Chalgren Anaerobe Agar. 1905 Wilkins-Chalgren Anaerobe Agar with Cysteine. 1905 WilkniN Chalgren Anaerobe Agar with G N Supplement, 1905 Wilkins-Chalgrcn Anaerobe Agar with N-S Supplement, 1906 Wilkins-Chalgrcn Anaerobe Broth. 1906 Wilkins-Chalgren Anaerobe Medium with Yeast Hxtracl. 1906 Wilkins-Chalgren Anaerobic HiVeg Agar Base with Blood, 1906 Wilkins-Chalgrcn Anaerobic HiVeg Agar Base with Blood and Nonspore Anaerobic Supplement, 1907 Wilkins-Chalgren Anaerobic HiVeg BroUi Base with Blood. 1907 Willaerlia magna. 1217 Williopsis californica, 589 Wilson and Blair's Medium. 1907 Wilson Blair Base. 1907 Wilson Blair I liVeg Agar Base with Selective Reagent and Brilliant Green. 1908 Wilson Blair HiVcg Agar with Brilliant Green and Selective Reagent. 1908 Wine. 917. 1802 Winemaking, 1573 WingeAgar, 1909 Winge Melibiose Agar. 1909 Winogradsky's Medium, Mtxlitied, 1909 Winogradsky's N-Frcc Medium. 1909 Winogradsky's Nitrite Medium. 1910 Without. 693 WL Differential Agar, 1910 WL DilTcrcntial HiVcg Agar. 1910 WL Differential HiVeg Broth, 1910 WL DilTcrcntial Medium. 1910. 1911 WL Medium with Tomato Juice, 1911 WL Nutrient Agar. 1911

WL Nutrient Broth, 1912 WL Nutrient HiVeg Broth, 1912 WL Nutrient HiVeg Medium. 1912 WMC Medium, 1912 Wolfe's Mineral Solution, 5 Wolfe's Vitamin Solution. 5 Wolin-Bevis Medium, 1913 Woiineila recta, 631 Woiineila species. 370, 701

Woiineila succinogenes. 1913 Woiineila succinogenes Medium, 1913 Woods and Welton Agar. 1914 Worfel-Ferguson Agar, 1914 Wort. 1867 Wort Agar, 1914 Wort Agar 6째Brix. 1914 Wort Broth. 1914 Wort HiVeg Agar, 1914 Wort HiVeg Broth. 1915 Wort Sucrose Agar. 1915 Wort Sucrose Broth. 1915 Wounds. 418 WSH Agar. 1901 X Xanthine Agar, 1915 Xanthine utilization, 1915 Xanlhohacteragilis, 830, 1916 Xanthobac/er agilis Agar. 1915 Xanlhobacler autotrophics, 643, 740. 741 Xanthobacter species. 740. 741, 749. 826. 827,828.829,1173 Xanthomonas Agar. 1916 Xanthomonas albilineans. 1653.1904. 1905. 1916, 1952 Xanthomonas albilineans Agar. 1916 Xanthomonas alblinieans, 1916 Xanthomonas axonopodis, 1904. 1916 Xanthomonas campestris, 489,748,827,828, 930,1367,1405,1412,1414,1916,1920, 1944. 1945 Xanthomonas campestris pvai. campestris. 826.829 Xanthomonas fragartae. 1273. 1916 Xanthomonas maltophilia, 1916 Xanthomonas maltophiiia Medium. 1916 Xanthomonas Medium, 1916 Xanthomonas oryzae, 1916, 1920, 1945 Xanthomonas species. 136.748.1000. 1309. 1905. 1916.1917, 1943 Xanthomonas Tryptone Yeast Mxtract Glucose Agar. 1916 Xanthomonas TYG Agar. 1916 Xanthoria parietina. 951 XB45/XB9OTB90-2 Medium, 1917 XI-D-AGAR. 1917 Xenococcus species. 152, 1200 Xenorhabdus Agar. 1918 Xenorhabdtts Broth. 1918 Xenorhabdus luminescens, 1484 Xenorhabdus nematophilus, 1369. 1918 Xerocvmus badius. 1002 Xeromyces bisporus, 988 Xerophilic molds, 593 XL Agar Base, 1918 XLDAgar. 1918, 1919 XLD Agar, HiVeg, 1919 XLT-4.1922 XLT4 HiVeg Agar Base. 1919 XPSAgar, 1920 XPS Broth, 1920

Index

XPS Broth with Thymidine, 1920 XSMAgar, 1920 Xylan Medium. 1921 Xylan utilization, 1921 Xylella Agar, 1921 Xylella faslidiosa, 471, 1450 Xylella fasiidiosa Medium, 1922 Xylella species, 1922 Xylophilus ampelina. 1922 Xylophilus ampelinus, 1916, 1943 Xylophilus Medium, 1922 Xylose fermentation, 1392.1919 Xylose Inclose Tergitolâ&#x201E;˘ 4. 1922 Xylose Lysine Agar Base. 1918 Xylose Lysine Deoxycholate Agar, 1918, 1919 Xylose Lysine Deoxycholate HiVeg Agar. 1919 Xylose Sodium Deoxycholate Citrate Agar. 1925 Xylose utilization, 1922. 1926 Xylose Yeast Lxtiact Peptone Agar, 1925 Xylose Yeast 1-xtract Peptone Broth. 1926 Xylose YP Agar, 1925 Xylose YP Broth. 1926

Yeast Extract Beef Extract Medium, 1924 Yeast Extract Dextrose Calcium Carbonate Agar. 1926 Yeast Hxtract Glucose Agar, 1928 Yeast F-xtract Glucose Broth, 1928 Yeast Extract Glucose Calcium Carbonate Agar, 1928 Yeast Hxtract Glucose Carbonate Medium, 1943.1944 Yeast Hxtract Glucose Carbonate Medium with Glutamic Acid, 1944 Yeast Extract Glucose Carbonate Peptone Medium, 1944 Yeast Tixiract Glucose Citrate Medium, 1944 Yeast Hxtract Glucose Citrate Medium with Cysteine, 1944 Yeast Hxtract Glucose Medium, 1929 Yeast Hxtract Glycerol Medium, 1929 Yeast Extract HiVeg Agar. 1929 Yeast Hxtract Malt Extract Agar, 885 Yeast Hxtract Malt Extract Agar. Diluted. 1/10, 1929 Yeast l^xlract Malt Hxtract Glucose Agar, \ 1929 Yeast Hxtract Mannitol Agar. 1930 Y 1 Adrenal Cell Growth Medium. 1924,1925 Yeast Hxtract Medium, 1930 Y 1 adrenal assay. 807 Yeast Hxtract Medium with Sodium Sulfide, Y 1 mouse adrenal cells, 1924 YA Halophilc Medium, 1924 1930 YA12,1923 Yeast Extract Mineral Agar, 1930 Yamadazyma stipitis, 19-41. 1942 Yeast I'xtract Mineral Medium, 1930 Yarrowia lipolytica. 1001, 1941. 1942. 1949 Yeast Hxtract Nutrient Agar Medium, 1948 YB Medium, 1924 Yeast Hxtract Nutrient Gelatin Medium. 1948 YC Agar without Tryptophan. 1925 Yeast Hxtract Peptone Beef Hxtract Medium, YC Medium without Tryptophan, 1925 1941 YDC Agar, 1926 Yeast Hxtract Peptone Starch Agar. 1930 YDC Medium, 1926 Yeast. 123. 128. 133. 232.233. 245.249. Yeast I'xtract Peptone Sulfate Cysteine Agar, 250. 254, 432, 849, 1002, 1222, 1530, 1951 1801, 1927,1932,1938 Yeast Hxtract Peptone Sulfate Cysteine MeYeast Agar. Van Niel's, 1926 dium. 1951 Yeast Agar, Van Niel's with 2.5% Sodium Yeast Hxtract Peptone Sulfate Cysteine Soft Chloride. 1926 Agar, 1952 Yeast Agar, Van Niels with 25% Sodium Yeast Hxtract Phosphate Agar, 1930 Chloride. 1926 Yeast I'xtract Powder. 6 Yeast Agar, Van NiePs with Glutamatc, 1926 Yeast Hxtract l*roteosc Peptone Medium. Yeast Agar, Van Niel's with Succinate, 1926 1942 Yeast Ascospore Agar, 1927 Yeast Autolysate Growth Supplement, 5 Yeast Hxtract Rose Bengal I liVeg Broth Base Yeast Beef Agar. 122. 131 with Sorbose, 1931 Yeast Beef Broth, 124 Yeast Hxtract Skim Milk Agar, 1931 Yeast Beef HiVeg Agar, 127 Yeast Hxtract Sodium Lactate Medium, 1931 Yeast Beef HiVeg Broth, 128 Yeast l-jciract Sucrose Agar, 1931 Yeast Carbon Base, I OX, 1927 Yeast Hxtract Tryptone Medium, 1952 Yeast Dextrose Agar, 1927 Yeast Hxtract Tryptone NaCl Medium. 1953 Yeast Dialysale, 5 Yeast Hxtract. 5 Yeast Fermentation Broth. 1932 Yeast Extract Agar, 1927,1928 Yeast fermentation HiVeg Broth Base with Yeast Hxtract Agar for SchizosaccharomyCarbohydrate, 1932 ces, 1928 Yeast fermentation Medium, 1932 Yeast Glucose Agar, 1932

2035

Yeast Glucose Agar for Acetobacter, 1932 Yeast Glucose Broth, 1933 Yeast Glucose Litmus Milk, 1945 Yeast Glucose Litmus Milk with Chalk. 1945 Yeast HiVeg Agar, 1933 Yeast Malate Medium, 1933 Yeast Malt Agar. 1934 Yeast Malt Agar for Arthrohacler viscosus, 1934 Yeast Malt Extract Agar, 1934 Yeast Malt Hxtract Broth. 1934 Yeast Mall Extract Broth with 0.5% Calcium Carbonate. 1934 Yeast Malt Hxtract Broth with 1.0% Methanol. 1935 Yeast Malt Hxtract Broth with 18% Sodium Chloride, 1935 Yeast Malt Hxtract Broth with 2.0% Calcium

Carbonate, 1934 Yeast Malt Hxtract Broth with 40.0% Sucrose, 1935 Yeast Malt Extract Broth with 70.0% Sucrose, 1935 Yeast Mall Extract Broth with Glucose. 1935 Yeast Malt Hxtract Catalase Agar, 1935 Yeast Malt HiVeg Agar, 884, 1936 Yeast Mall HiVeg Broth. 1936 Yeast Mannitol Agar, 1936 Yeast Mannitol Agar, Modified. 1936 Yeast Milk Medium, 1936 Yeast Morphology Agar. Unbuffered. 1936 Yeast Nitrogen Base, 1937 Yeast Nitrogen Base Glucose Brotb. 1938 Yeast Nitrogen Base with Carlxihydratc, 1937 Yeast Nitrogen Base, 10X with Aspaiagine ami Glucose. 1937 Yeast Peptone Agar, 1938 Yeast Peptone Broth. 1938 Yeast Peptone Dextrose Agar, 1949 Yeast Peptone Salt Medium, 1938 Yeast phase, 850, 1528 Yeast Starch Agar, 1939 Yeast Starch Agar A. 1939 Yeast Starch Agar. Diluted 1/10, 1939 Yeast Starch Agar, Half Strength. 1939 Yeast Synthetic Medium with 5-dI MP, 1939 Yeast Synthetic Minimal Medium, 1939, 1940 Yeast Tryptone Medium, 1940 Yeast Tryptone Medium with Streptomycin, 1940 Yeast Tryptone Starch Medium, 1940 Yeast Water Agar, 1940 Yeastolate, 6 Yeast-Peptone-Succinate Medium, 1938 Yeastrel Agar. 1940 Yeasts, 54, 55, 59,136. 137,151,223. 284. 313, 318,353, 591, 607, 692, 998, 1001, 1002. 1003.1214.1261,1337. 1403, 1411. 1412.1413,1414,1415. 1416.

2036

Index

1417, 1517, 1530, 1531, 1533, 1552, 1867,1868, 1878,1910,1911, 1912, 1914,1915, 1929. 1932,1934. 1937. 1938

Yeasts susceptibility test, 1937 YED Medium. Salted. 1940 YEME Iliiostrepton Medium, 1941 YEP Agar. 1930 YEP Galactose Agar, 1941 YEP Galactose Medium. 1941 YEPB Medium. 1941 YEPDAgar, 1941 YEPD Inositol Agar, 1942 YEPD Medium, 1942 YEPD Medium with 0.3.V/Sodium Chloride, 1942 YEPD Medium with Heme. 1942 YEPD-FA Medium, 1941 YEPG Medium, 1942 YEPG with 0.5% Calcium Chloride, 183 YEPP Medium. 1942 Yersinia enterocolitica, 104. 218, 291. 292. 335, 388, 445, 446,995, 1227, 1350, 1426.1447. 1481.1625. 1931. 1943 Yersinia Isolation Agar. 291 Yersinia Isolation HiVeg Agar, 1943 Yersinia pestis. 222, 1350 Yersinia pseudotuberculosis, 854, 1350, 1405,1931 Yersinia Selective Agar. 334.388 Yersinia Selective Agar Base, 1943 Yersinia Selective HiVeg Agar Base with Selective Supplement. 1943 Yersinia Selective Supplement, 7 Yersinia species. 444.446.458, 879. 887. 1310,1365, 1463 YESA. 1931 YGB. 1933 YCIC Medium, 1943,1944 YGC Medium with Cysteine, 1944 YCJC Medium with Glutamic Acid. 1944 YGCB Salt Medium, 1944 YGCP Medium. 1944

YGI.M. 1945 YGI.M with Chalk, 1945 YGI.PB Medium, 1945 YGI.PB Medium without Lactase, 1945 YI-S Medium, 1945 YM Agar. 1934 YM Broth, 1934 YM Broth with 1.0% Methanol. 1935 YM Broth with 18% NaCl, 1935 YM Broth with 40.0% Sucrose. 1935 YM Broth with 70.0% Sucrose. 1935 YM Broth with Glucose, 1935 YM Calalase Agar. 1935 YM HiVeg Agar. 1936 YM HiVeg Broth, 1936 YM Medium. 1946 YM-IE Broth. 1946 YM5 with 10% Sorbitol, 1947 YMAAgar. 1947 YMA Medium, 1947 YMF Agar. 1947 YMI Broth. 1947 YNA Medium, 1948 YNG Medium. 1948 Yogurt, 438, 941, 970, 986. 987 Yolk Milk Agar, 1948 YOM, 1948 Yopp's Medium, 1948 YP87 Medium, 1948 YPAD Medium for MAK Mutants of Saccharomycet, 1949 YPC Medium. 1949 YPI) Medium, 1949 YPDA. 1949 YPDP Medium with 5-TMP. 1949 YPG Agar with 2% Sodium Chloride, 1950 YPG Medium, 1950 YPGA, 1950 YPM Agar, 1950 YPNC Medium, 1950 YPS Medium, 1951 YPSC Agar, 1951 YPSC Agar. Cation Supplemented, 1951

Y1ÂťSC Medium. 1951 YPSC Soft Agar. 1952 YpSs Agar, 1952 YPSS, Kmerson Agar. 1952 YSP Agar. 1952 YT HiVeg Broth, 1952 Yl Medium. 1952 YTG Medium, 1952 YIN Medium, 1953 YTSS Medium, Hall" Strength, 1953 /.

/.Agar, 1953 /Broth, 1953 / Medium, 1953 /Mvarzinellafortnoso, 1954 YMvarzinellafortnosa Medium, 1953 Za\<arzinia compransoris. 314 ZF2 Medium, 1954 Zobellia uliginosa, 1558 Zwfgloea Medium, 1955 Zoogloea mmigera, 643,1938, 1955 Zoogioea species. 1955 Zopfiella pleuro))ora. 944 Zygomonas mobilis. 1155 Zygosaccharomyces bailii, 136. 137, 1332. 1941, 1942 Zygosaccharomyces bisporus, 1332 Zygosaccharomyces rouxii. 136. 137, 589, 750,988, 1001, 1332, 1534,1935, 1941,

1942 Zymobacierium Agar. 1955 Zynwbacterium Broth. 1955 Zymomonas Agar, 1955 Zymomonas anaerobia, 1955 Zymomonas Medium, 1955 Zymomonas mobilis, 1039, 1515, 1955 Zymomonas sj)ecies, 136, 1940, 1955 Zymomonas Sucrose Medium. 1955 Zymophilus paucivorans, 1236. 1456 Zymophilus rqffinosivorans. 1236.1456