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Anaerobic Microbiolog y Table of Contents 1 Introduction to AnaeroGRO™ 3 Plates - AnaeroGRO™ 5 Tubes - AnaeroGRO™ 6 Anaerobic Transport Media 7 AnaeroGen™ Gas Generators 9 CampyGen™ Gas Generators 11 CO2Gen™ Gas Generators 13 Anaerobe Jar 14 Identification Disks and Rapid Test Reagents
A Culture of Service ™ Headquarters 1430 West McCoy Lane Santa Maria, CA 93455 Phone: 800.266.2222 Fax: 805.346.2760 Sales@HardyDiagnostics.com www.HardyDiagnostics.com Distribution Centers Santa Maria, California Olympia, Washington Salt Lake City, Utah Phoenix, Arizona Dallas, Texas Springboro, Ohio Lake City, Florida Albany, New York Raleigh, North Carolina
The Quality Management System at the Hardy Diagnostics manufacturing facility is certified to ISO 13485. IAGNOST ICS RDY D HA
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Copyright © 2014 Hardy Diagnostics
Roo m Temperature Storage!
aPre-reduced (ready-to-use) culture media packaged in oxygen-free, gas-flushed foil pouches aContains an oxygen scavenger sachet and a moisture absorbing desiccant packet aFoil pouches with easy-to-open, pre-scored notch aWide variety of packaging combinations available: monoplates, biplates, and primary set-up combinations aRoom temperature storage
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aQuality Control tested for reliable and reproducible results aExceptional growth and performance characteristics compared to other brands aManufactured in an ISO 13485 certified facility aCompetitive pricing, quality products, and exceptional service and support aTubes with screw-cap design and rubber septum Hungate caps
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Easy-to-open Pre-scored Notch
Oxygen Impermeable Foil Pouch
Flushed with Oxygen-free Gas inside bag
One, two, three, or four plate combinations available.
Oxygen Scavenger Sachet absorbs residual O2 after gas flushing
Moisture-absorbing Desiccant
* Do not refrigerate. Room temperature storage only.
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One Plate Per Foil Pouch
Description
Cat. no.
Pack Size
Anaerobic PEA (Phenylethyl Alcohol) Agar (monoplate)
AG901
1 plate/pk
BBE (Bacteroides Bile Esculin) Agar/LKV (Laked Blood, Kanamycin, and Vancomycin) Agar (biplate)
AG061
1 plate/pk
BBE (Bacteroides Bile Esculin) Agar (monoplate)
AG051
1 plate/pk
Brucella Agar with Hemin & Vitamin K (monoplate)
AG301
1 plate/pk
Campy Selective Agar (monoplate)
AG701
1 plate/pk
CCFA (Cycloserine Cefoxitin Fructose Agar) (monoplate)
AG501
1 plate/pk
Egg Yolk Agar, Modified (monoplate)
AG401
1 plate/pk
LKV (Laked Blood, Kanamycin, and Vancomycin) Agar (monoplate)
AG601
1 plate/pk
“The next time we need to place an order, it will be Hardy. Our side-by-side comparison was unbelievable. The CLS couldn’t believe how much better the organisms grew on your media. With the cost savings, it’s a no-brainer.” Pat Wong Alta Bates Medical Center 3
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Two Plates Per Foil Pouch Description
Cat. no.
Pack Size
DuoPak A Brucella Agar with H & K (monoplate), BBE Agar/LKV Agar (biplate)
AG302
2 plates/pk
DuoPak B Brucella Agar with H & K (monoplate), BBE Agar/Anaerobic PEA Agar (biplate)
AG312
2 plates/pk
Cat. no.
Pack Size
MultiPak A Brucella Agar with H & K (monoplate), Anaerobic PEA Agar (monoplate), BBE Agar/LKV Agar (biplate)
AG303
3 plates/pk
MultiPak B Brucella Agar with H & K (monoplate), Anaerobic PEA Agar (monoplate), LKV Agar (monoplate)
AG313
3 plates/pk
Cat. no.
Pack Size
AG304
4 plates/pk
Three Plates Per Foil Pouch Description
Four Plates Per Foil Pouch Description Brucella Agar with H & K Brucella Agar with H & K (monoplate)
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Tube Media
Filtered to Remove Dead Bacteria. Reduces False Positives!
Needleport Septum (Hungate Cap)
Anaerobic environment inside the tube
Description
Cat. no.
Pack Size
Thioglycollate Broth with H & K, without Indicator, Hungate cap, 16x125mm tube, 9ml fill
AG22H
20 tubes/pk
Thioglycollate Broth with H & K, without Indicator, Hungate cap, 16x125mm tube, 7ml fill
AG23H
20 tubes/pk
Chopped Meat Glucose Broth, Hungate cap, 16x125mm tube, 9ml fill
AG19H
20 tubes/pk
Chopped Meat Carbohydrate Broth, Hungate cap, 16x125mm tube, 9ml fill
AG20H
20 tubes/pk
Chopped Meat Broth, Hungate cap, 16x125mm tube, 9ml fill
AG21H
20 tubes/pk
PY Glucose Broth, Hungate cap, 16x125mm tube, 9ml fill
AG24H
20 tubes/pk
Filtered to Remove Dead Bacteria. Reduces False Positives!
Non-Filtered Media
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Anaerobic Transport Media
Needleport Septum, (Hungate Cap)
The Anaerobic Transport System is a sterile, ready-to-use collection kit available, with or without swabs.
• • • • •
Media is semi-solid and pre-reduced. The sterile peel pouch is suitable for the surgical suite. The medium has an indicator that will turn lavender if the anaerobic condition is compromised. Fluids can be injected with a syringe directly into the tube through a pierceable rubber septum. The media maintains viability of a wide variety of microorganisms for up to 48 hours during transportation. Patient Information
Anaerobic Transport Medium
Includes two collection swabs, scored for easy break-off into the transport tube. (Cat. no S120D only)
Description
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Cat. no.
Pack Size
Anaerobic Transport Medium, sterile surgery pack, Hungate septum screw-cap, no swab, 15x103mm glass tube, 7ml fill
S1200
100 tubes/case
Anaerobic Transport Medium, sterile surgery pack, Hungate septum screw-cap, two swabs, 15x103mm glass tube, 7ml fill
S120D
100 tubes/case
Anaerobic Transport Tube, non-sterile pouch, Hungate septum screw-cap, no swab, 15x103mm glass tube, 7ml fill
AG25H
Anaerobic Microbiology
20 tubes/pk
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AnaeroGen™ AnaeroGen™ Compact (Bag System) AnaeroGen™ Compact is a simple-to-use
• Sachet activated upon opening
system for rapidly generating an anaerobic
• Final atmosphere with less than 1% oxygen,
environment necessary to cultivate anaerobic microorganisms. The AnaeroGen™ Compact system consists of an AnaeroGen™ sachet, oxygen impermeable pouch, and sealing clip. Each pouch is large enough to hold four 15x100mm petri plates. • No catalyst needed and no water required for
which is ideal for the growth of anaerobes • Anaerobic conditions reached in 30 minutes
AnaeroGen™ Compact System
Sachets and Pouches 10/bx. ......................................................AN010C Sealing Clips 5/bx (reusable) ........................................AN005C Anaerobic Indicator 100/bx ............................................................BR55
activation • No potentially explosive hydrogen gas produced • No dangerous build-up of pressure, as sachet acts as an oxygen scavenger
Sealing Clip
AnaeroGen™ Compact Sachet O2 Impermeable Pouch
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AnaeroGen™ Anaerogen™ Jar System for Anaerobes
AnaeroGen is a simple-to-use system for rapidly generating an anaerobic environment necessary to cultivate anaerobic microorganisms within a sealed jar. The AnaeroGen™ system consists of an AnaeroGen™ sachet and an oxygen indicator within a gas jar.
AnaeroGen™ Jar System
™
• No catalyst needed and no water required for activation • No potentially explosive hydrogen gas produced • No dangerous build-up of pressure, as sachet acts as an oxygen scavenger • Sachet activated upon opening • Final atmosphere with less than 1% oxygen, which is ideal for the growth of anaerobes • Anaerobic conditions reached in 30 minutes
AnaeroGen™ Sachet
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AnaeroGen™ 2.5 liter Sachets 10 sachets/bx ..........................................AN25US AnaeroGen™ 3.5 liter Jar 10 sachets/bx ..........................................AN35US Almore Gas Jar, 2.5 liter Each ..............................................................16000 Anaerobic Indicator 100/bx ...........................................................BR55
Almore Gas Jar
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CampyGen™ CampyGen™ Compact (Bag System)
CampyGen™ Compact is a simple-to-use system for rapidly generating a microaerophilic environment necessary to cultivate Campylobacter spp. The CampyGen™ Compact system consists of a CampyGen™ sachet, an oxygen impermeable pouch, and a sealing clip. Each pouch is large enough to hold four 15x100mm petri plates. • • • • •
No catalyst needed and no water required for activation No potentially explosive hydrogen gas produced No dangerous build-up of pressure, as the sachet acts as an oxygen scavenger Sachet activated upon opening Final atmosphere with 6% oxygen, which is ideal for the growth of Campylobacter and other microaerophilic organisms • Microaerophilic conditions reached in 30 minutes
CampyGen™ Compact System Sachets 20 sachets/bx............................................CN020C
Sealing Clips 5/bx (reusable)..........................................AN005C
Plastic Pouches 20 pouches/bx..........................................AG020C
CampyGen™ Compact Sachet
CampyGen™ Compact Sachet
Sealing Clip O2 Impermeable Pouch
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CampyGen™ CampyGen™ (Jar System for Campy)
CampyGen™ is a simple-to-use system for rapidly generating a microaerophilic environment necessary to cultivate Campylobacter spp. within a sealed jar. The CampyGen™ jar system consists of a CampyGen™ sachet and a gas jar. • No catalyst needed and no water required for activation • No potentially explosive hydrogen gas produced • No dangerous build-up of pressure, as the sachet acts as an oxygen scavenger • Sachet activated upon opening • Final atmosphere with 6% oxygen, which is ideal for the growth of Campylobacter and other microaerophilic organisms • Microaerophilic conditions reached in 30 minutes
CampyGen™ Jar System
Campy Gas Tank
A 20 cubic feet disposable gas tank contains microaerophilic gas (5% O2, 10% CO2, 85% N2) for the filling of gas impermeable bags in culturing Campylobacter spp. Shipping requires a hazmat shipping charge. One tank will fill about 100 single plate bags. Campy gas bag, oxygen impermeable plastic, zip-lock: 6”x6”, 250/bx..............................................4742S 9”x12”, 250/bx............................................4744S Campy gas tank, Each........................CG100 Campy gas tank, nozzle, brass, reusable, Each........................TN100 Wall mounting bracket, Each.......................S0735
CampyGen™ 2.5 liter Sachets 10 sachets/bx............................................CN025A Almore Gas Jar, 2.5 liter Each..............................................................16000
CampyGen™ Sachet
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Almore Gas Jar
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CO2Gen™ CO2Gen™ Compact (Bag System)
CO2Gen™ Compact is a simple-to-use system for rapidly generating an increased CO2 environment ideal for growing capnophilic microorganisms such as Neisseria gonorrhoeae and Haemophilus influenzae. The CO2Gen™ Compact system consists of a CO2Gen™ sachet, oxygen impermeable pouch, and sealing clip. Each pouch is large enough to hold four 15x100mm petri plates. • Sachet activated upon opening • Final atmosphere with 6% carbon dioxide, which is ideal for the growth of capnophilic organisms • Capnophilic conditions reached in 30 minutes
CO2Gen™ Compact
20 sachets/bx.........................................CD020C Plastic Pouches 20 pouches/bx.......................................AG020C Sealing Clips 5/bx (reusable).......................................AN005C CO2 Indicator (IndiKwik) 100 strips/bx...............................................G511
Sealing Clip
Impermeable Pouch
CO2 Gen™ Sachet
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CO2Gen™ CO2Gen™ (Jar System for Capnophilic Microorganisms)
CO2Gen™ Jar System
CO2Gen™ is a simple-to-use system for rapidly generating an increased CO2 environment ideal for growing capnophilic microorganisms within a sealed jar. The CO2Gen™ system consists of a CO2Gen™ sachet and a gas jar. Each sachet generates enough gas for a 2.5 liter jar. • Sachet activated upon opening • Final atmosphere with 6% carbon dioxide, which is ideal for the growth of capnophilic organisms • Capnophilic conditions reached in 30 minutes
CO2Gen™ 2.5 liter Sachets 10 sachets only..........................................CD025A Almore Gas Jar, 2.5 liter Each.............................................................16000 CO2 Indicator (IndiKwik) 100 strips/bx..................................................G511
Almore Gas Jar
CO2 Gen™ Sachet
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Anaerobe Jar The Almore anaerobe jar is specially designed for temperature stability and durability. It is unbreakable, lightweight, transparent, and autoclavable. A double screw clamp, coupled with the patented Quad-Ring™ gasket, ensures a more positive seal superior to conventional O-rings. The screw clamp lid also doubles as a handy carrying handle. The 3/8 inch thick anodized aluminum lid will not warp. It is guaranteed to resist cracking or breaking for ten years. This product is shipped complete with a stainless steel rack to accommodate 14 standard sized petri plates (15x100mm). With stainless steel rack and catalyst basket................................................................................16000
Classic! Durable! Proven! Reliable!
Solid, durable, screw locking lid Long-lasting anodized aluminum lid Quad-Ring™ gasketbest seal in the industry
Holds 14 plates
Stainless steel rack for easy plate removal, holds 14 petri plates Gas-generating sachet
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Identification Disks and Rapid Test Reagents Vancomycin, 5μg, HardyDisks™
For the differentiation of anaerobic bacteria based on susceptibility to vancomycin. Contains 50 disks that are spring-loaded for smooth and reliable dispensing, by Hardy Diagnostics. 50disks/cartridge........................................Z7501
Kanamycin, 1000μg, HardyDisks
™
For the differentiation of anaerobic bacteria on the basis of susceptibility to kanamycin. Contains 50 disks that are spring-loaded for smooth and reliable dispensing, by Hardy Diagnostics. 50 disks/cartridge.......................................Z7191
Anaerobe Nitrate Reagent A
Sulfanilic acid for nitrate reduction test, by Hardy Diagnostics. 15ml, Each.....................................................Z134
Anaerobe Nitrate Reagent A, Sulfanilic Acid for nitrate reduction test, Z134.
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Anaerobe Nitrate Reagent B
Cleve’s Acid for nitrate reduction test, by Hardy Diagnostics. 15ml, Each......................................................Z135
Nitrate Reagent C
Zinc dust for nitrate reduction test, by Hardy Diagnostics. 5gm...................................................................Z73
Bile HardyDisks™
To aid in the identification of the Bacteroides fragilis group on the basis of bile resistance, by Hardy Diagnostics. 50 disks/cartridge.......................................Z7091
Bile HardyDisks™, Cat. no. Z7091
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Indole Nitrate Broth
Catalase Reagent, 15%
A 15% solution of hydrogen peroxide for the detection of catalase activity in anaerobic bacteria, by Hardy Diagnostics. 15ml dropper bottle............................................Z262
Colistin Sulfate (CT), 10μg, HardyDisks™
For the differentiation of anaerobic bacteria based on susceptibility to colistin, by Hardy Diagnostics. 50 disks/cartridge.............................................Z8411
Indole Nitrate Broth
For the detection of nitrate reduction and indole production by microorganisms with a wide range of oxygen tolerances inculding aerobes, microaerophilic, facultative anaerobes, and obligate anaerobes. Contains a Durham tube for gas detection, by Hardy Diagnostics. 6ml, 16x100mm tube with Durham tube, 20/pk..............................................................K147
Showing negative nitrate reduction reaction after the addition of Nitrate Reagent A (Cat. no. Z134) and Reagent B (Cat. no. Z135). Clostridium sordellii grown in Indole Nitrate Medium (Cat. no. K147).
Showing positive nitrate reduction reaction after the addition of Nitrate Reagent A (Cat. no. Z134) and Reagent B (Cat. no. Z135). Propionibacterium acnes grown in Indole Nitrate Medium (Cat. no. K147).
Nitrate, HardyDisks™
For the determination of nitrate reduction by anaerobes, by Hardy Diagnostics. 50 disks/cartridge............................................Z7071
Showing a positive nitrate reduction reaction. Propionibacterium acnes (ATCC® 11827) colonies growing on Brucella Agar with H and K (Cat. no. A30) with a Nitrate HardyDisk™ (Cat. no. Z7071) aseptically placed on the plate prior to anaerobic incubation (24 hours at 35oC). One drop of Nitrate Reagent A (Cat. no. Z71) and one drop of Nitrate Reagent B (Cat. no. Z72) were dropped onto the disk. The red color development was indicative of a positive reaction.
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A 1.5ml aliquot of the sample transferred to a clean test tube. Showing a negative indole reaction after the addition of Indole Reagent (Cat. no. Z67). Clostridium perfringens grown in Indole Nitrate Medium (Cat. no. K147).
A 1.5ml aliquot of the sample transferred to a clean test tube. Showing a positive indole reaction after the addition of Indole Reagent (Cat. no. Z67). Clostridium sordellii grown in Indole Nitrate Medium (Cat. no. K147).
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SPS Disks
For identification of Gardnerella vaginalis and Peptostreptococcus anaerobius, each cartridge contains 50 disks that are spring-loaded for smooth and reliable dispensing, by Hardy Diagnostics. 50 disks/cartridge........................................Z7381
Nitrocef MatchBook™
Nitrocef Matchbook™
The Nitrocef MatchBook™ is a rapid test (5 minutes) used to quickly pick colonies and perform the test at the same time, easier and faster than a disk! The rapid Nitrocef test consists of a paper match-stick impregnated with the nitrocefin reagent. A positive ß-lactamase test can predict resistance to such antibiotics as penicillin, ampicillin, amoxicillin, and other ß-lactamaseproducing organisms: • • • • • •
Single Disk Dispenser
Compatible with all HardyDisks™ Each............................................................260457
Neisseria gonorrhoeae Haemophilus spp. Moraxella (Branhamella) catarrhalis Staphylococcus spp. Enterococcus spp. Anaerobes (such as some species of Bacteroides, Porphyromonas, Clostridium, Fusobacterium, Prevotella)
Nitrocef Matchbook, 10 tests/pk.......................................................Z108 Nitrocef Disks, 50/vial...........................................................Z7301
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Now with
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• Over 13,000 laboratory supply products are stocked and ready for immediate shipment. • Utilize our online ordering at www.HardyDiagnostics.com. • Fresh media - longer shelf life • Rapid one or two-day ground delivery.
9 Distribution Centers 2 Manufacturing Plants
to serve you better! Santa Maria, California Olympia, Washington Salt Lake City, Utah Phoenix, Arizona Dallas, Texas Springboro, Ohio Lake City, Florida Albany, New York Raleigh, North Carolina
Same Day Shipping Orders placed before 1:30 p.m. for all time zones (except Hawaii and Alaska) are processed and shipped out the same day. Most orders are received the next morning.
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Online Ordering Call for more information 800.266.2222 ext.5696 1430 West McCoy Lane Santa Maria CA 93455 © Hardy Diagnostics, 2014
For Rapid and Inexpensive Delivery - Take Advantage of our Nationwide Distribution Network and Dual Manufacturing Facilities!
1 or 2 day ground delivery service zone.
To learn more about our new products, view our online web catalog. Contact us for a sample or scan QR code to request sample. IAGNOST ICS RDY D HA
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Distribution Centers: California | Washington | Utah | Arizona Ohio | Texas | New York | Florida | North Carolina
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