shimadzu - Chromatographs

Chromatographs Chromatography is the standard analytical separation technology used for quantitative analysis. Ever since Shimadzu developed its ďŹ rst gas chromatograph in 1956, we have continued to broaden our chromatography lineup, by providing liquid chromatographs and systems combined with mass spectrometers. Meanwhile, we have developed a variety of application-speciďŹ c systems designed for customers in the pharmaceutical, environmental, food and chemical industries.

Shimadzu also provides data processing and data management software to actively help customers comply with various regulatory requirements, including GXP and FDA 21 CFR Part 11.

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GC

GCMS

HPLC/UCLC/UHPLC

LCMS

LCMS-IT-TOF

LCMS/MS

High-Performance Liquid Chromatograph (HPLC) HPLC systems are able to quantitatively analyze substances in mixtures containing multiple ingredients by separating and detecting target substances. They are also able to purify specific substances once they are separated. The Prominence is a modular HPLC system, which can be configured to create the optimal system for specific customer applications, while the integrated LC-2010HT is standardized for routine analytical applications. Shimadzu also offers a wide variety of other application-specific systems, such as automated sample pretreatment systems for separating metabolites contained in pharmaceuticals or biological samples, residual pesticide sample pretreatment systems for separating pesticides, and systems for analyzing organic acids and amino acids.

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Prominence (Modular HPLC) LC-2010HT (Integrated HPLC)

LC-2010HT Compact LC for Ease of Use, High Sample Capacity for Routine Applications Applications Pharmaceuticals Food & Beverage Environmental Biotechnology

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Ultra-High Speed LC - Prominence UFLC Ultra High Performance Liquid Chromatograph Shimadzu’s passion has always been to improve performance, ease of use and flexibility for all HPLC environments. That passion continues with Nexera, the next era in UHPLC, which meets all analysis requirements with no compromise in data integrity. At 19,000 psi (130MPa), and with such features as the world’s fastest injection, near-zero carryover, and excellent injection reproducibility, the modular Nexera is ideally suited to investigate minute quantities of impurities in a range of application areas - from pharmaceuticals and academic research to environmental and food/beverages.

Nexera™ follows in the renowned footsteps of the Prominence ultrafast LC and UFLCXR. Capable of system pressures in excess of 9500 psi (66MPa) with the choice of seven bonded phases, the Prominence UFLCXR delivers powerful high-pressure analysis-without sacrificing performance. Utilizing standard components, Prominence UFLC raises total system productivity by generating high-quality, reproducible data without compromise.

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Nexera (Ultra High Performance LC) g Prominence UFLC (Ultra Fast LC) Prominence UFLCXR (Ultra High Pressure UFLC) g Shim-pack XR-ODS Columns Prominence nano (Nano-flow LC)

Nexera Ultra High Performance Liquid Chromatograph Applications Pharmaceuticals Food & Beverage Environmental Biotechnology

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Preparative Liquid Chromatography System (Prep LC) Scale-up from analytical to preparative LC is a critical factor in order to conserve solvent, and increase efficiency and productivity of purification. The LC-20AP pump contributes to a highly precise scale-up workflow since it has excellent basic performance over a wide flow range from analytical to preparative flow rates, enabling the Shimadzu prep system to be utilized in applications ranging from purification of natural products to impurity analysis. In addition, the LC-20AP provides substantial space and energy savings.

Shimadzu prep LC features are: t Easy scale up from analytical to prep t High-resolution purification with gradient elution t High pressure tolerance (6000 psi, 42Mpa) enables use of high-resolution columns t Wide flow rate range (settable 0.01–150 mL/min)

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LC-20AP Preparative System Large Scale Preparative System (Automated Scale-up System) High-Performance Semi-Preparative Recycle System Bio-Chemical Analysis/ Preparative System (Inert LC System)

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Preparative LC System

Applications Purification of natural/synthesis compounds Pharmaceuticals Food & Beverage Chemical 09

Liquid ChromatographMass Spectrometers (LCMS) The LCMS-2020 is utilizing patent pending ultrafast (UF) technology, the LCMS-2020 has faster measurement speed and signiďŹ cantly higher sensitivity than any other single quadrupole analyzer. This provides more accurate detection of trace impurities in pharmaceuticals, environmental pollutants and other contaminants. The new UFscanning technology achieves mass spectrum measurement speeds of 15,000 u/sec without sacriďŹ cing sensitivity or resolution, thus obtaining the best chromatography for the fastest LC conditions. The novel UFswitching technology achieves industry-leading 15 ms polarity switching, enabling accurate data from even the fastest chromatographic peaks without any loss of peak height.

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LCMS-2020 (ESI Ion Source as standard) APCI Ion Source (Optional) DUIS-2020 (Simultaneous ESI and APCI Ion Source / Optional) Preparative LCMS

LCMS-2020 Liquid Chromatograph Mass Spectrometer Applications Pharmaceuticals Food & Beverage Enviromental Biotechnology

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LCMS-IT-TOF ® The LCMS-IT-TOF ® is a system which combines an ion-trap mass spectrometer (IT) with a time-of-flight mass spectrometer (TOF).

The ion trap offers MS n capability and the TOF provides high-resolution and highly accurate MS analysis capability. This combination offers the diverse analysis information required for effective structure analysis in such research areas as impurity analysis, Metabolomics profiling and biomarker discovery. In addition, Shimadzu offers user-friendly, application-specific software products to maximize the performance of the LCMS-IT-TOF ®.

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LCMS-IT-TOF® APCI/APPI Ion source (Optional) Nano ESI Interface (Optional) Software Packages Formula Predictor, MetID Solution, Profiling Solution, Open Solution Component ID

LCMS-IT-TOF® Hybrid High Performance Liquid Chromatograph-Mass Spectrometer Applications Metabolite Identification Impurity Analysis Natural Product Analysis Protein Identification

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World Fastest* Triple Quadrupole Mass Spectrometer The LCMS-8030 combines the power of triple quadrupole mass spectrometry with unmatched speed to provide the ideal complement to its UHPLC systems.

The LCMS-8030 features ultrafast multiple reaction monitoring transitions, enabling data acquisition with up to 500 different channels per second. The improvements to the electronics provide ultrafast mass spectrum measurement speeds of 15,000 u/sec without sacrificing sensitivity or resolution, and ultrafast polarity switching (15msec) for the most information without signal deterioration. UFsweeper™, a unique technology created by Shimadzu, accelerates ions out of the collision cell by forming a pseudo-potential surface.

The result is high-efficiency collision-induced dissociation and ultrafast ion transport, reducing the sensitivity losses and cross talk observed on other systems. The LCMS-8030’s robust design allows maximum uptime, resulting in a system that can handle most complex matrices. Maintenance of the desolvation line without breaking vacuum minimizes instrument downtime. *Based on our survey (as of August 2010)

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NEW LCMS-8030

APCI-8030 (Optional) DUIS-8030 (Optional)

LCMS-8030 Triple Quadrupole Mass Spectrometer Applications Pharmaceuticals Food & Beverage Environmental Biotechnology Chemical 12

Gas Chromatographs Gas chromatographs analyze samples qualitatively and quantitatively. After evaporation of the sample, the different components are separated on a column and subsequently sent to the detection system. By comparing with samples of known concentrations, the concentration in an unknown sample can be determined. Shimadzu GC-2010 Plus enables reliable trace analysis utilizing detectors such as FID and FPD that feature best-in-class sensitivity. Newly introduced Advanced Flow Technology reduces analysis times, provides enhanced chromatographic resolution, and enables application-specific configurations, such as multidimensional GC, backflush, and detector splitting and switching systems, without compromising performance. The GC-2014 can be used for both capillary and packed column analysis. Fully configured systems to address a range of analysis requirements, from environmental and petrochemical to biofuels and greenhouse gases, are available.

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GC-2010 Plus (Capillary GC)

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GC-2014 (Capillary/ Packed GC) Advanced Flow Technology Devices (Multidimensional GC/ Heart-cut/ Backflushing/ Detector Splitting/ Detector Switching)

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GC-2010 Plus Gas Chromatograph Applications Petrochemical Industry Environmental Flavor & Fragrance Biofuels

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Gas Chromatograph-Mass Spectrometers (GCMS) GCMS systems are gas chromatographs coupled with mass spectrometers, which excel at identification of compounds. Shimadzu’s quadrupole contributes to increasing sensitivity and higher productivity in the laboratory. Newly-designed electric control platform provides best-in-class scanning speed and the advanced scanning speed protocol (ASSP) ensures sensitivity improvement at high-speed data scanning. Features for shorter analysis time and easy maintenance increase efficiency. Library search using Linear Retention Indices and Automatic Adjustment of Retention Times (AART) make identification easy and reliable. In addition to standard models with electron ionization (EI), Shimadzu also offers models with Chemical Ionization (positive and negative) for enhanced selectivity as well as sensitivity. A Direct sample introduction system (DI) is available as an option. Shimadzu also offers a broad range of accessories including a Thermal desorption system (TD-20), the AOC-5000 autosampler for liquid, headspace and SPME injection as well as a cooled PTV (temperature programmable injector) (Optic 3), a Pyrolysis (Py-2020) and Shimadzu Advanced Flow Technologies for Heart-Cut GC/GCMS. Shimadzu’s fast quadrupole with up to 100 scans/s enables the system to perform Comprehensive GCMS, using thermal modulation (Zoex loop modulator) for narrowest peak shape and highest sensitivity.

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GCMS-QP2010 Ultra, available models : EI, EI/CI, EI/CI/NCI (upgradable) GCMS-QP2010SE (Standard EI) Direct Injection (DI) (Optional)

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TD-20 (Thermal desorption system) AOC-5000 (liquid, headspace, SPME) Optic 3 (PTV) Zoex thermal loopmodulator (Comprehensive GCMS)

MDGC/GCMS-2010 Ultra Multidimensional GC/MS System Applications Enviromental Flavor & Fragrance Petroleum Industry Forensics RoHS/WEEE/ELV 14

Multidimensional Chromatography Multidimensional methods allow the analysis of complex samples in a few steps using standard hardware (LC and/or GC) with some special parts for the modulation between the dimensions. Beside the coupling of several columns with the same properties for highest resolution, combinations of chemically different stationary phases are also used. These approaches enable the increase of the peak capacity and can help to avoid co-elution as can happen in single dimension chromatography. In HPLC and GC practice different concepts are used and common: t QFBL DVUUJOH BOE UIF TFMFDUJWF USBOTGFS PG QFBLT PS chromatogram sections onto a second column (heart-cut method) t DPOUJOVPVT UXP EJNFOTJPOBM DISPNBUPHSBQIZ DPNQSFIFOTJWF chromatography)

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MDGC-2010 MDGCsolution Software LC-GC(MS) System LCxGC Software

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Comprehensive LC(MS) Comprehensive GC(MS) GCImage Software ChromSquare Software

LC-GC (MS) System Multidimensional Chromatography Applications Food Flavor & Fragrance Polymers Enviromental

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Energy-Saving Capillary Gas Chromatograph Shimadzu’s new-generation GC-2025 capillary gas chromatograph minimizes environmental impact by reducing power and carrier gas consumption while retaining the performance capabilities required for capillary analysis. The GC-2025 incorporates a digital ow controller that controls both the carrier and detector gases and a newly designed energy-saving column oven that features small volume and less heating loss, realizing a dramatic improvement in operation costs. The compact GC-2025 is the gas chromatograph for environmentally friendly, high value performance.

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NEW GC-2025 (Capillary GC) Automatic liquid sample injection system, AOC-20 Series (Optional) Low temperature control solenoid valve, CRG-2025 (Optional)

GC-2025 Energy-Saving Capillary Gas Chromatograph Applications Petrochemical Industry Environmental Food & Beverage Academia

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Gas Integrated LC/GC Workstation Software Integrated chromatography software for HPLC/GC systems, LabSolutions Ver. 5.3 supports automation of all LC workflows from aout startup and system check to baseline check, auto purge, and auto shutdown, making analysis workflow more efficient. Now with integrated LC/GC control, the LabSolutions provides a unified, easy-to-use operating environment for increased laboratory productivity and customized operation.

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NEW LabSolutions Ver. 5.3

LabSolutions Software Integrated Chromatography Workstation Features Enhanced GLP/GMP support FDA 21 CFR Part 11 compliance (Optional) Customized operating environment Comprehensive quantitative results window Powerful, secure data management LC/GC control on a common platform — a maximum of 16 systems can be registered 17