Nalin bhatia ehs bls101w

BLS 101W

Bloodborne Pathogens Training

Module 1: General Compliance

Module 2: Biological Safety

Module 3: BBP Standard

Module 1: General Compliance 1A: Recombinant DNA Requirements (Level 2) 1B: Risk Groups and Containment Levels 1C: Routes of Exposure and Aerosols 1D: Biological lab emergencies and reporting

Module 2: Biological Safety Module 3: BBP Standard

Module 1: General Compliance Module 2: Biological Safety 2A: Basic Biosafety 2B: Housekeeping, Wastes, Spills 2C: Engineering Equipment

Module 3: BBP Standard

Module 1A:

Recombinant DNA Requirements (Level 2)

Click on the DNA source to generate the chromosomal DNA fragment

DRAG

Chromosomal DNA fragment to be cloned

Cut DNA Molecule

Recombinant DNA Molecule

Drag the DNA fragment towards the cut DNA molecule to generate a recombinant DNA molecule.

DRAG

Chromosomal DNA fragment to be cloned

Cut DNA Molecule

Recombinant DNA Molecule

Click on recombinant DNA molecule to see the two possibilities

Stays on the vector

Integrates into the host genome

Which roles and responsibilities apply to recombinant DNA research as outlined in NIH guidelines? Select all that apply If one lab at the University of Michigan receives NIH funding, not all labs are necessarily required to comply with the NIH Guidelines.

The UM-IBC promotes assessment of potential risks, and reduces personnel exposure or accidental environmental release IBC will review recombinant DNA research, assess the biosafety containment level proposed At least BL1 lab practices should be used with all recombinant DNA work Non compliance with the NIH guidelines may result in loss of funding DNA research to be submitted directly to the NIH for approval before work can be conducted DNA research can submitted to the NIH even after work begins

Drag to the Principal Investigator’s responsibilities to the appropriate place as per the sequential order Responsible for registering their research with the Institutional Biosafety Committee Determine the required levels of physical and biological and select the appropriate microbiological practices and laboratory techniques for research.

Responsible for reporting any significant problems or violations of the NIH Guidelines.

Communicate with the IBC throughout the project regarding subsequent changes or amendments to an approved IBC registration.

During to Research

Prior to Research 1.

Responsible for registering their research with the Institutional Biosafety Committee

1.

2. Responsible for full compliance with the NIH guidelines ensuring that no research is initiated prior to IBC approval and that all other requirements of the NIH Guidelines are met.

2.

3.

3. Report any significant research related accidents and illnesses to the Biological Safety Officer, Animal Facility Director, IBC, NIH/OBA or other applicable authorities.

Viral Vectors

Adapted from https://www.youtube.com/watch?v=-Rt_NlVSEDk

Which of the following are responsible for the Select Agents and Toxins program - to prevent the loss, theft or misuse of microorganisms, biological materials, and research-related information.

Choose the correct virus based on the given trait:

Adenovirus

Epstein barr virus

Lentivirus

Can integrate into Transduced cells with high Have ecotropic envelope frequency cannot absorb efficiently to Member of the herpes virus human cells family

Adenoassociated virus

Can cause severe infections in persons with underlying skin conditions, pregnant women and immunocompromised individuals

Retrovirus

Moloney Murine Leukemia Virus

Vaccinia virus

Have ecotropic envelope cannot absorb efficiently to human cells

Key: Adenovirus

mild respiratory illness, pink eye or gastroenteritis

Adeno-associated virus

dependent for replication

Epstein barr virus

member of the herpes virus family

Retrovirus

can integrate into transduced cells with high frequency

Lentivirus

subset of retroviruses

Moloney Murine Leukemia Virus

Have ecotropic envelope - cannot absorb efficiently to human cells

Vaccinia virus

can cause severe infections in persons with underlying skin conditions, pregnant women and immunocompromised individuals

Which of the following are identified as Dual Use Research NSABB Categories? Select all that apply.

Reduces the risks of a biological agent or toxin.

Disrupts the immunity or the effectiveness of an immunization without clinical and/or agricultural justification.

Confers to a biological agent or toxin resistance to clinically and/or agriculturally useful prophylactic or therapeutic interventions Does not lead to dissemination of a biological agent or toxin Alters the host range or tropism of a biological agent or toxin Eradicates a pathogenic agent.

Module 1a: Recombinant DNA Requirements (Level 2)

End of Section Quiz

The State of Michigan, Michigan Occupational Safety and Health Administration (MiOSHA) regulations are the only regulations required to be followed if your work does not involve recombinant or synthetic nucleic acid molecules: The NIH Guidelines for Recombinant or Synthetic Nucleic Acid Molecules states that at least BL1 lab practices should be used with all recombinant DNA work Viral vectors are not as hazardous as wild type viruses because a portion of the viral genome has been removed, and therefore are safe for use at a lower containment level (BSL1) because there are fewer risks associated with them.

True

False

True

False

True

False

Who is responsible for providing lab specific training, that is specific to the agents and techniques for the biological research being conducted in the lab?

OSEH representative that provides annual training The PI, lab manager, or other designee who is familiar with the lab operations Lab personnel who is conducting the research The Department in which you work

Module 1B:

Risk Groups and Containment Levels

Choose the which risk groups the given organisms fall under:

Group 1 •

B. subtilis

•

M. Tuberculosis

•

West Nile virus

•

Vaccina virus

•

Lassa

•

Herpesvirus simiae

•

E. coli K12 strains

•

S. Aureus

•

Y. Pestis

•

Ebola

•

E. coli O157H7

•

S. cervisiae

Group 2

Group 3

Group 4

Select the appropriate standard microbiological practices to be followed:

Select the appropriate standard microbiological practices to be followed:

You should wash hands after working and before leaving lab

Select the appropriate standard microbiological practices to be followed:

You should not consume any food in lab!

Click on a lab component label on the left. Then choose the ri with its respective name (CLICKED) BIOLOGICAL SAFETY CABINET AUTOCLAVE CHEMICAL FUME HOOD

You are required to work in a safety training lab. Which of the following should NOT be worn as PPE?

Module 1C:

Routes of Exposure and Aerosols

You have accidentally come in contact with a contaminant through skin. What are the measures to be undertaken to reduce risk? Select all that apply.

Vigorous hand washing with mild soap.

Use waterless hand disinfectant or antiseptic towelettes preferably instead of washing with soap and water.

Dry hands thoroughly Use emergency eyewash All of the above

You have accidentally come in contact with a contaminant through skin. What are the measures to be undertaken to reduce risk? Select all that apply.

Nose or Mouth rinse well (Be careful of choking) Wash eyes with disinfectant Use emergency eyewash Immediately flush eyes for 15 minutes Obtain immediate medical attention

Module 1D:

Biological Lab emergencies and Reporting

While working in the lab, you accidently cut your finger with a sharp object. What are the measures to be undertaken to reduce risk? Select all that apply.

Wash exposed area with disinfectant Report suspected or confirmed exposures within 24 hours.

Use waterless hand disinfectant or antiseptic towelettes preferably instead of washing with soap and water.

Wash exposed area with soap and water for 10 minutes.

Obtain immediate medical attention Rinse well your nose and mouth

Module 2A:

Basic Biosafety

Which is responsible for reviewing all experiments involving recombinant or synthetic nucleic acid molecules, human gene transfer experiments and research involving Select Agents or which requires biosafety level 3 containment?

Institutional Biosafety Committee (IBC)

Compliance is required with which of the following. Select all that apply.

Institutional Biosafety Committee (IBC)

Ensures proper approvals for research involving biological research with animals models?

Institutional Biosafety Committee (IBC)

There are several safety compliance violations in the given image. Chose the ones you think are violating the safety standards.

Correct! You should wear fluid proof shoe covers.

There are several safety compliance violations in the given image. Chose the ones you think are violating the safety standards.

Correct! Lab wastes should not be disposed off in regular trash cans.

There are several safety compliance violations in the given image. Chose the ones you think are violating the safety standards.

Incorrect! Lab coats are required to be worn.

Drag the biohazard warning label to the appropriate items.

Label the given images correctly

1.

2.

Wear Protective Clothing Chemical Storage Area Wear Face Protection No eating or drinking Wear Safety Glasses Biohazard Radioactive Material

3.

4.

5.

6.

7.

Module 2B:

Housekeeping, Wastes, Spills

Follow the questions and chose the best option for each step You just noticed a BBP spill from a Glass capillary tube. After donning personal protective Equipment, what are you supposed to do next?

Chose the most suitable item to pick up the test tube

Now that the sharp object is removed, click the disinfectant bottle and drag it towards the spill. Now right click to pour the disinfectant. Based on the course, use the correct manner to pour the disinfectant.

Now drag the paper towel from the right and place it correctly over the spill to let it saturate for 15-20 minutes.

Now click the disinfectant bottle again and re-spray the entire area.

Wipe the area by dragging the paper towel again.

DRAG

Dispose off the waste in the appropriate bag

Module 2C:

Engineering Equipment

Chose the correct image of the equipment from the description provided.

Designed to protect the product from contamination, but not considered lab safety equipment.

Laminar flow cabinet Correct! A Laminar Flow Cabinet is recommended for work with nonhazardous materials where clean, particle-free air quality is required

Has High Efficiency Particulate Air (HEPA) filters

Biological safety cabinet Correct! A Biological safety cabinet has High Efficiency Particulate Air (HEPA) filters which only traps particulates, allowing any contaminant in non-particulate form to pass through the filter

Designed to limit exposure to hazardous or toxic fumes, vapors or dusts.

Fume Hood Correct! Fume hoods simply remove the toxic agents from the work area and exhausts them to the outdoors to limit exposure.

Module 3:

Blood borne pathogens (BBP)

Choose the which bio-contaminant safety level the given organisms fall under: High risk •

Sweat

•

Dental Saliva

•

Vaginal Secretions

•

Semen

•

Fecal Material

•

Vomit

•

Any body material with visible blood

•

Urine

•

Cerebrospinal Fluid

•

Saliva(non-dental)

•

Peritoneal fluid

•

Tears

•

Pericardial fluid

•

Amniotic Fluid

Low risk

In which of the following ways do you think HIV can be transmitted?

Handshakes

Unprotected Sexual Intercourse

Injections

Sharing Utensils

HBV and HCV have similar symptoms whereas HIV has some distinguishable symptoms. Drag the symptom icons To their correct disease column Yellowish skin

Flu like symptoms

Loss of appetite Fever

Rashes Night sweat

Muscle and joint pain Rapid weight loss

Dark urine

Vomiting

HBV and HCV

HIV

Drag the slider to the correct interval of percent chances of contracting the given virus infections.

HCV

0

10

20

30

40 2-10%

(from needle)

HBV

6

30

54

78

100

0.1

0.2

0.3

0.4 0.5

(from needle)

HIV

0.3

(from needle)

HIV (from eyes, nose and mouth)

30-54%

0.2

0.3

0.4 0.5

0.4

Although needle recapping is not advisable, in case if there is no feasible alternative for recapping, what are the correct ways recapping should be done? Select all that apply.