Phytofabrication Of Nanoparticles from Lamiaceae Against Infectious Microbes 1
Neelam Jain*1 and G. K. Aseri2, Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur - 303 002 2Amity Institute of Microbial Technology, Amity University Rajasthan, Jaipur Email ID: neelam.aseri@gmail.com
INTRODUCTION
OBJECTIVES
Synthesis of Nanoparticles by Physico-chemical techniques is complex, costly and toxic •Preparation of plant leaf extract which can lead to environmental pollution. Therefore biological sources are an alternative •Phytofabrication of Nanoparticles for the synthesis of functional nanoparticles. Plants being the richest natural source of bioactive molecules have been used in traditional and modern medicine. Phytofabrication of nanoparticles is a possible ecofriendly alternative to chemical and physical method. Lamiaceae is an important family of medicinal and aromatic plants, consisting of about 160
•Characterization of NPs (SEM, UV-Vis Absorption, FTIR, XRD) •Antimicrobial
activity
of
plant
extract
and
phytofabricated
Nanoparticles against infectious microbes by Agar well Assay •Preliminary phytochemical analysis of plant extract PHYTO-FABRICATION OF SILVER NPs AND THEIR MODE OF ACTION
species with 24 species native to India. Their bioactive essential oils are being used as pharmaceutical agents. This study is comparative account of plant mediated nanoparticles synthesis and their characterization from different members of Lamiaceae for antibacterial SRI
activity against infectious microbes. METHODOLOGY Preparation of leaf extract 10 Gm Of Washed Fine Cut Fresh Leaves + 100 mL Double Distilled Water
Boiled For 5 Minutes At 80°C
Green Synthesis of Ag NPs 5 mL Of Leaf Extract + 95 mL Of 1 mM Aqueous AgNO3 Solution
Heated For 5 Minutes At 80°C
Extract Cooled
Extract Cooled Colour Change Indicating Formation Of Silver Nanoparticles
The Filtrate Was Centrifuged For 10 Minutes At 10000 Rpm
Filtered With Whatman No 1 Filter Paper
Supernatant Collected And Stored At 4°C
Repeated Centrifugation At 10000 Rpm For 15 Minutes
Dried And Purified Silver Nanoparticles
CONT
CONCLUSIONS •Screening for phytochemicals revealed the presence of various bioactive secondary metabolites like alkaloids, tannins, flavonoids, glycosides, triterpenoids and sterols which probably contribute to its antimicrobial properties. •The important ingredients responsible for the formation of silver nanoparticles present in the leaf extract are triterpenes, flavonoids and phenols. •Hence members of Lamiace can be used to discover lead bioactive natural products for formulation of new drugs in treatment and prevention of various healthcare related infections. •Applications of such eco-friendly nanoparticles in bactericidal, wound healing and other medical and electronic applications makes this method potentially exciting for the large-scale synthesis of other nano materials. •Ablend of traditional knowledge alone or along with modern techniques like nanotechnology can be a possible substitute for synthetic antibiotics in combating infectious diseases in future for improving public health worldwide.
Characterization OF AgNPs
Table : Phyto-fabrication And Antimicrobial Activity of Silver NPs From Lamiaceae Against Infectious Microbes