Phytofabrication Of Nanoparticles from Dried Mango Powder Against Food Borne Pathogens 1
Neelam Jain*1 and G. K. Aseri2 Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur - 303 002 2Amity Institute of Microbial Technology, Amity University Rajasthan, Jaipur Email ID: neelam.aseri@gmail.com OBJECTIVES
INTRODUCTION
•Preparation of dried mango powder extract
Nanoparticles synthesis by Physico-chemical techniques is complex, costly and toxic
•Phytofabrication of Nanoparticles
which can lead to environmental toxicity. Therefore Synthesis of NPs through biological
•Characterization of NPs (Particle size analyzer, UV-Vis Absorption,
sources can prove as suitable alternative. The traditional and folkore medications use
FTIR, XRD)
plants as the natural source of bioactive molecules. Thus green synthesis of nanoparticles
•Antimicrobial activity of extract and phytofabricated Nanoparticles
is a possible ecofriendly alternative to existing methods. Mango (Mangifera indica L.)is an
against infectious microbes by Agar well Assay
important fruit and dried mango powder is frequently used in Indian kitchens. This study provides an insight of dried mango powder and its green synthesized silver nanoparticles as pharmaceutical agents for antibacterial activity against five important food borne
•Preliminary phytochemical analysis of dried mango powder extract PHYTO-FABRICATION OF SILVER NPs AND THEIR MODE OF ACTION Dried Mango Powder
1mM Silver Nitrate Soln.
infectious microbes. The size of silver NPs was 22.5nm as determined by particle size
4
absorbance
3 2
analyzer. The Ag NPs were characterized for their UV absorption spectra, FTIR and XRD.
1 0 0
METHODOLOGY Preparation of leaf extract 10 Gm Of dried Mango powder + 100 mL Double Distilled Water
Powder is Stirred and boiled by using magnetic stirrer for 2-3hrs
200
400
600
800
RESULTS
Green Synthesis of Ag NPs
•Dried mango powder extract and its silver nanoparticles were synthesized using green route
10 mL Of Mango powder Extract + 90 mL Of 1 mM Aqueous AgNO3 Solution
•Screening for phytochemicals revealed the presence of various bioactive secondary metabolites like tannins, flavonoids, glycoside and sterols which probably contribute to its antimicrobial
Stirred by using magnetic stirrer for 3-4 hours
properties. Extract Cooled
•NP size was 22.5 nm as determined by Particle size analyzer
Extract Cooled Colour Change Indicating Formation Of Silver Nanoparticles
The Filtrate Was Centrifuged For 10 Minutes At 10000 Rpm
Filtered With Whatman No 1 Filter Paper
•Both the extract of dried mango powder and its silver nanoparticles were effective against five test food borne microbes
Repeated Centrifugation At 10000 Rpm For 15 Minutes
•Maximum antibacterial efficacy was reported against E.coli followed by Pseudomonas aeruginosa. Dried And Purified Silver Nanoparticles
Supernatant Collected And Stored At 4°C
•Hence extract of dried mango powder and its silver nanoparticles can be used in treatment and prevention of various food borne infections.
Characterization OF AgNPs
Table : Preliminary Phytochemical Analysis of Dried Mango Powder Sr. no
Compounds
1
Carbohydrate
+
2
Flavonoids
+
3
Glycosides
++
Figure : Antibacterial activity of dried mango powder extract and its AgNPs against food borne pathogens
Graph : UV-Vis Absorption spectra of AgNPs of Dried Mango Powder
Dried Mango powder 35
4
30
3.5
25
4
Fixed oils and fats
5
Tannins and phenolic compounds - By dilute ferric chloride sol. -1% sol. Of gelatin with 10%Nacl
-
3
6
7
Proteins -Millon’s reagent - Biuret test Steroids
Absorbance
20
2.5
+ +
15
+ -
10
1.5
5
1
2
+ 0.5
8
Alkaloids - Dragondroff’s Reagent -Hager’s reagent
-
0 Pseudo
E.Coli
Klebsiella
S.aureus
Shigella 0
Series 1
Series2
Series 3
0
Series 4
100
200
300
400
500
600
700
800
Wave Length (in nm)
CONCLUSION
REFERENCES
•Mango powder and its AgNPs can combat food borne pathogens
1. Thambi, P.A., John, S, Lydia, E, Iyer, P, Jane M.S (2016). Antimicrobial efficacy of
especially the enteric disease causing microbes.
mango peel powder and formulation of recipes using mango peel powder (Mangifera
•A blend of traditional knowledge alone or along with modern
indica L.). Int J of Home Sci; 2(2): 155-161
techniques like nanotechnology can be a possible substitute for
2. Gupta, C., Garg, A.P. and Gupta, S (2010). Antimicrobial and Phytochemical Studies
synthetic antibiotics in combating food borne infectious diseases in
of Fresh Ripe Pulp and Dried Unripe Pulp of Mangifera indica (Amchur). Middle-East
future for improving public health worldwide.
Journal of Scientific Research 5 (2): 75-80