Nanopaprica' 2012
Nanopaprica' 2012
Detection of total count of Staphylococcus aureus using anti-toxin antibody labelled gold magnetite nanocomposites: a novel tool for capture, detection and bacterial separation Dipshika Bhattacharya and Prof. P. Pramanik* Indian Institute of Technology, Kharagpur, INDIA Background: Food-borne disease is one of the world’s most pressing health challenges as the low infectious dose of pathogenic bacteria in food and contaminated water not only presents a serious threat to human health but also causes widespread damage for decades‌ Aim: Fast, sensitive and specific detection of S.aureus at ultra low concentrations using multifunctional antibody labelled-gold-magnetite nanocomposites exploiting the molecular recognition property of bacterial antigen and its against antibody
Scheme
Fabrication strategy of multifunctional -Au-Fe3O4 probes
Schematic demonstration of pathogen detection by multifunctional Au-Fe3O4 Nanocomposites.
Results and Discussions
Conclusions This nanoprobes could detect S.aureus within 30 minutes within a range of 102-107 CFU/ml with its low limit of 102 CFU/ml. This nanoprobes could specifically recognise S.aureus from pathogenic mixture.
Bibliography 1. S. Guo, S. Dong , E. Wang, Chem. Eur. J., 2009, 15, 2416. 2. C. Wang, J. Irudayaraj small., 2010, 6, 283.