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Nano Si lverAssembled Conventi onalMetalSurfacefor Mari neAnti fouli ng Appli cati ons D.I nbakandan1, 4,V.Si vakumar2,DuneNagaRavi ndra2,C.Kumar1,R.Venkatesan3,S.Aj malKhan4 1. CentreforOcean Research,SathyabamaUni versi ty,Chennai600119,I ndi a 2. DepartmentofBi otechnology,SathyabamaUni versi ty,Chennai600119,I ndi a 3. Nati onalI nsti tuteofOcean Technology,Palli karanai ,Chennai 600036,I ndi a 4. CentreofAdvanced Studyi n Mari neBi ology,AnnamalaiUni versi ty,Parangi pettai608502,I ndi a

Abs t r a c t As i mpl ewe t c he mi c a lt e c hni que f or t he a s s e mbl y of s i l v e r na nopa r t i l c e s on c onv e nt i ona l me t a ls ubs t r a t e s , whi c ha l l e v i a t e s t hene e df or l i t hogr a phy , ha sbe e n de v e l ope d.S i l v e r na nopa r t i c l e s we r epr e pa r e dbyt hebi ol ogi c a l r e duc t i onofs i l v e rpr e c ur s or sa nd a t t a c he dt os i l a nef unc t i ona l i z e d S t a i nl e s ss t e e l s ur f a c e st oc r e a t ea S e l f As s e mbl e Monol a y e r wi t h a nt i mi c r of oul i ngpr ope r t i e s .T he a s s e mbl i e sofs i l v e rna nopa r t i c l e s ons ur f a c e swe r ec ha r a c t e r i z e dby S E Ma ndE ne r gyDi s pe r s i v eXr a y S pe c t r os c opy . T he s i l v e r na nopa r t i c l e s di a me t e r wa s e s t i ma t e d by T E M a na l y s i s . De t e c t i on a nd me a s ur e me ntof t he e ne r gy pe r mi t se l e me nt a l a na l y s i sofs ur f a c er e v e a l e dt he c oa t i ng na t ur e a nd s i l v e r na nopa r t i c l e sde pos i t i on. Cont a c t a ngl eme a s ur e me nt sr e v e a l e dt he hy dr ophobi cna t ur ea nd s ur f a c e e ne r gyoft hepr e pa r e ds ur f a c e s . T hi swor kr e por t e dt he ma r i ne bi ol m f or ma t i on on s ur f a c e s e x pos e dt os e a wa t e ri nv i t r of or10 da y s . Af t e rr e t r i e v a l f r om s e a wa t e r t hes ur f a c e swe r es c a nne d wi t h e l e c t r on mi c r os c ope a nd t he i ma ge sa r ec ompa r e df orma r i ne bi ol m f or ma t i on. Bi ol m ba c t e r i a la t t a c hme nt s on t he s ur f a c e swe r es t udi e dbya c r i di ne or a nge s t a i ni ng a nd l i v e / de a d Ba c l i ght uor e s c e nc e s t a i ni ng. L owma r i nebi ol mf or ma t i ona nd hy dr ophobi cna t ur equa l ie st he modie d s ur f a c e f or ma r i ne a nt i f oul i nga ppl i c a t i ons . CONTACT:

Dr . DI nba k a nda n, S c i ent i s t– C Cent r ef orOc ea nRes ea r c hS a t hy a ba ma Uni v er s i t yChenna i -600119, T a mi l Na du, I ndi a . E ma i l : i nba k a nda n@gma i l . c om Ph: 9144-24500646F a x914424500646 Mobi l e: 919965540310

B i o s y n t h e s i so f S i l v e r Na n o p a r t i c l e s S p e c i e s: Ac e n t h e l l ae l o n g a t a

1 0 mlo fa q u e o u ss p o n g ee x t r a c twa s a d d e dt o1 0 0 mlo f1 0 −3 M Ag NO3 a q u e o u ss o l u t i o na n dk e p ta t4 5 ° Cf o r4 h o u r si nc o n t i n u o u ss t i r r i n g .

Ac o l o r c h a n g et oy e l l o wi s hb r o wn c o nr me d t h e b i o s y n t h e s i so f s i l v e r n a n o p a r t i c l e s .

XRD anal ysi sar e assi gned t ot he cr yst al st r uct ur eoft hef acecent er cubi c( f cc)si l ver .

F T I Rs pec t r um oft hes pongeex t r a c tofA. el onga t as howspea k sa t3255,2039,1628, 1006a nd662c m−1 T heF T I Rs pec t r um ofs i l v erna nopa r t i c l es bi os y nt hes i z ed by s ponge ex t r a c tofA. el onga t as howspea k sa t3345,2078,1634, 1031a nd627c m−1

C h a r a c t e r i s a t i o no f S i l v e r N a n oP a r t i c l e s Ab a n do b s e r v e di nU V V i s i b l e s p e c t r u m c o r r e s p o n d i n g t o t h e S u r f a c eP l a s mo nR e s o n a n c e o c c u r s a t 4 2 4n m

HR T E Mg r a p h sr e v e a l e dt h e d i ff e r e n ts i z e so ft h es i l v e r n a n o p a r t i c l e s ,r a n g i n gf r o m 1 5n mt o3 4n mi nd i a me t e r

H i s t o g r a m r e v e a l e da l mo s t 2 0 %o ft h ep a r t i c l e swe r e 2 4n mi nd i a me t e r

T i t a ni um

S i l a na s ed

S NPc oa t ed

S E MI MAGE Conc l us i on:

Ma r i nebi ol mf or ma t i onons ur f a c esex pos edt o s ea wa t eri nv i t r of or10da y s .Af t err et r i ev a lf r om s ea wa t ert hes ur f a c eswer es c a nnedwi t hel ec t r on mi c r os c opea c r i di neor a nges t a i ni nga ndl i v e/ dea d Ba c l i ght uor es c enc es t a i ni ng.L ow ma r i nebi ol m f or ma t i on a nd hy dr ophobi cna t ur e qua l iest he modieds ur f a c ef orma r i nea nt i f oul i nga ppl i c a t i ons


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