IV
III
160
160
3
2
2
1
1
0
LDH levels (% maximum)
3
0 Ctrl 0.9 1.9 3.7 7.5 15
5
30
60
Ctrl 0.4 0.8 1.5 3.1 6.3 12.5 25 50 5
Concentration (mg/L)
4
Concentration (mg/L)
4
3
3
2
2
1
1
0
0 Ctrl 0.8 1.5 3.1 6.3 12.5 25 50 100
140
120
120
100
100
80
80
60
60
40
40
120
20
160
30
60
0.8
140
120
120
3.1
6.3 12.5 25
50
Concentration (mg/L)
40
40
20
20
20
0
0
0
6.3 12.5 25
50
120
120
120
100
100
100
80
80
80
60
60
60
40
- NAC
40
40
20
+ NAC
20
20
80 400
100
2.3
4.7
9.4 18.8 37.5 75
100
10
100
Ag 23 nm at 50 mg/L Ag 25 nm at 10 mg/L Ag 27 nm at 30 mg/L
200
2.0 150 20
1.5 1.0
0 6
24
48
6
Time (hours)
0 Ctrl 0.8 1.6
120
3.1 6.3 12.5
X Data
800
100
600
80 60
400
40 200 20 0
0
10
24
48
180
0 Ctrl 0.5 0.9 1.9 3.8 7.5 15 30
Ag NPs
co-culture
140 120
transwell insert
100 80 60
Total Ag mea measured
40 20 0 1.6
/L /L /L /L /L /L mg 0 mg mg 5 mg mg 0 mg 3 2 1 0.6 1.9
Ag 25 nm Ag 27 nm
Ag NP concentration (mg/L)
IV Ø
Ø Ø Ø
mucus
160
Ag 23 nm
μ
0
Concentration (mg/L)
250 Ag 23 nm at 1 mg/L Ag 25 nm at 1 mg/L Ag 27 nm at 1 mg/L
0.0 1
200 20
200
Ctrl 0.2 0.3 0.6 1.3 2.5 5
150
10
Concentration (mg/L)
40
II
2.5
0
0
400
60
60
0.5
10
600
100
60
3.1
800
X Data
120
40
Ag content (ug/L)
140
1
Ctrl 0.2 0.7 2.2 6.7 20 60
60
1.6
0
140
80
3.0
140
100
0
Concentration (mg/L)
140
10
200 20
100
III
1
40
80
Ctrl 1.2 2.3 4.7 9.4 18.837.5 75 150
600 80
400
60
100
I
Metabolic activity (% of untreated control)
1.6
160
Concentration (mg/L)
140
800
100 600
0 Ctrl 0.9 1.9 3.8 7.5 15
120
800
80
20
0
Concentration (mg/L)
0
MTS TEER
100
TEER (Ohms*cm2)
- NAC + NAC
140
4
Metabolic activity (% untreated control)
5 - NAC + NAC
4
L) Ag content in basolateral compartment (ug/L)
5
Fold increase in LDH release (compared to untreated control)
II
I
TEER (Ohms*cm2)
I
Metabolic activity (% untreated control)
II
I