P15-07 by andras paszternak

QbD enabled Xanthan gum capped green synthesis of Optimized Gold nanoparticles. Alam Md Sabir, Salim 1. 2. 3.

1 M.A ,

2, Yadav Ashutosh

Javed Md

3** Noushad .

Luqman College of Pharmacy Fortis healthcare Jamia Hamdard University

* Presenting Author & Corresponding Author Corresponding email: rxnoushad@gmail.com INTRODUCTION

3D SURFACE PLOT

During recent years the field of nanotechnology focus on gold nanoparticle which has led to earlyphase clinical trials as promising therapeuticals , photothermals, diagnostic, contrast media , radiosensitisers agents. There is also growing interest in utilization of gold nanoparticles as drug delivery of existing drugs especially cytotoxic drugs, to improve its pharmacokinetics parameters by reducing its non-specific side effects and to enable higher dose payload in affected tissues. Conventional method for synthesis of Gold nanoparticles utilize various chemicals which are very toxic for normal cells which limit its use, so here emphasis are now a day’s towards synthesis of Gold Nanoparticle through green route. Many natural agents however are explored in past as reducing agent including citric acid but these were not only lack in terms of stability but also they can’t hold drug with it so there use as in clinical study are not possible. Xanthan gum (XG) here selected which have dual role as reducing and stabilizing agent. These gums are also widely use in food, pharmaceutical and cosmetic industries because these are safe for internal consumption due to its high biocompatibility and boil degradability. In the present study, we explored the potential of XG in the synthesis of gold nanoparticles and effect of various formulation and process variables such as temperature, gum concentration, and gold concentration, are tested using QbD approach for synthesis of optimized Gold Nanoparticles.

DEFINING QTPP, CQAs II,& CMAs Risk estimation matrix Formulation Volume Temprature Gold volume Gold Concentration Gum Volume Gum Concentration Heating Time Post formulation heating time Particle Size Low High Low High Medium High High High Zeta Potential Low High Low High Low High High High Stablity Medium High Low High Medium High High Medium CQAs

DESIGN OF EXPERIMENT

Particle Size = -209.78942 +260.55000* Gum+1.87375* Gold +0.17125 * Temp -3.61500* Gum * Gold –1.8300* Gum * Temp +0.11175 * Gold * Temp = -11.04 +0.83* A+2.4* B-0.76* C Zeta Potential

Optimised Particle Size & Potential

RESULT & DISCUSSION The stabilized Xanthan gum capped gold nanoparticle was synthesized via green chemistry and impact of various parameters on their performance were tested using QbD approach for optimized formulation. These functionalized nanoparticles were found to be more safer than other chemical reduced gold nanoparticle and can be further utilize in many applications in the area of which are including but not limited to chemotherapy, radiotherapy, electronics, theronostic , material science, nanorobots , analytical chemistry. QbD approach was used to understand how various factor influence the size and potential in the final formulation and so would help to reproduce desired and optimized formulation, here Gold nanoparticle size was found to be 78 nm by TEM, Average particle size was found to be 102 nm and zeta potential was -14.3mV. References: 1. Stephanie D. Steichen, Mary Caldorera-Moore, Nicholas A. Peppas, A review of current nanoparticle and targeting moieties for the delivery of cancer therapeutics. Eurapion gernal of pharmaceutical sciences; (2013). 416-427. 2. Dung The Nguyen, Dong-Joo Kim, Kyo-Seon Kim. Controlled synthesis and bimolecular probe application of gold nanoparticles. Micron. (2011). 207-227.