Biosynthesis of Silver Nanoparticles (Ocimum sanctum) by Green Synthesis Method Rajesh Kumar
a Meena ,
Pallavi
b Sharma
and Amit
c Kotiya*
aDepartment
of Pure and Applied Chemistry, University of Kota, Kota-324005 bModi Institute of Management & Technology, Kota, Rajasthan cDepartment of Botany , Rajasthan University, Jaipur, Rajasthan Corresponding Email Address: 1988rajeshmeena@gmail.com
SAED
X-ray diffraction pattern
INTRODUCTION
Tea Silver Nanoparticles 38.09nm
Intensity/a.u
In this research work, we report the synthesis of silver nanoparticles by using a green synthesis method. The 40 nm sized AgNPs synthesized via this one-pot greener approach, can be used as a capable material in different fields such as cosmetics, foods, medicine as well as pollutant degradation, etc. This environment benign process did not use of any extra capping or reducing agent or template. As synthesized silver nanoparticles were evidenced by some advance analysis techniques like as: Fourier transform infrared (FTIR) spectroscopy, UV-Vis spectroscopy, powder X-ray diffraction (PXRD) and transmission electron microscopy (TEM) etc. X-ray analysis exhibits that the pure silver nanoparticles were grown in a single phase. Particle size was confirmed by the XRD and TEM images of the well dispersed sample. This method can also be facilitated for other metals such as copper (Cu), palladium (Pd), gold (Au) and platinum (Pt).
500
(A) 29.68nm
44.12nm 64.37nm
30
40 50 60 Wavelength/nm
70
The XRD pattern of silver nanoparticles is shown in the figure represents the crystalline nature and purity of the silver nanoparticles. The XRD spectrum analysis indicated three diffraction peaks at 29.68o , 38.09o , 44.12o and 64.37o. It crystallised in cubic crystal (space group Fm-3m) pattern matches well with standard JCPDS file No. 04-0783. Keywords: Tea plant leaves, Green synthesis method, Capping agent, Bioreduction, AgNPs, etc.
Energy dispersive X-ray 120
x 0.001 cps/eV Ag-L
100
80
Transmission electron microscopy 60
Synthesis of AgNPs
Ag
Si
Ag
40
20
0 0.5
1.0
1.5
2.0
2.5
keV
3.0
3.5
4.0
4.5
5.0
CONCLUSIONS ➢A simple green synthesis method is developed at various near-room temperatures for the preparation of Ag Nanoparticles.
UV-Vis spectroscopy
The Transmission Electron Micrograph image indicate the ➢The average crystallite size of the Ag Nanoparticles dispersity and morphology of the silver nanoparticles. These was observed as the aspect ratio reached its particles are rounded in shape and distributed in smaller and maximum at higher temperatures, and decreased bigger size zones. These silver nanoparticles may be due to when moving toward lower temperatures. the capping of phytochemicals such as polyphenols.
Particle Size
➢The TEM image and SAED pattern confirmed that the prepared spherical silver nanoparticles are single crystals.
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It is well known that a characteristic absorption peak in Uv- visible spectroscopy for Ag nanoparticles appear around 440 nm of the optical range due to surface Plasmon resonance.
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