MEAT, FISH & POULTRY
Thermo Scientific Salmonella Precis Method simplifies workflows and encompasses challenging food matrices
Salmonella testing drives efficiencies,
enables flexibility and solves problems A quick and easy culture-based method for the enrichment, detection, and confirmation of Salmonella species has been validated for the broadest range of food, animal feed, and environmental samples, and is ready to slot into standard laboratory workflows.
E
Bernd Hofmann, VP Thermo Fisher
xtension of the validation scope for
including challenging samples such
the Thermo Scientific Salmonella
as cocoa and chocolate (up to 375g),
explains, “Our customers work in a pressured
Precis Method according to
infant formula (with and without
environment, handling many different types
ISO 16140-2 brings the opportunity for
probiotics), infant cereals, and related
of samples, with varying timelines and
increased efficiency by eliminating the need
ingredients (up to 375g), and animal feed
demands. We want to help them do all they
for multiple broths, plates, supplementation,
(up to 150g).
can to streamline processes, become more
and incubation temperatures. Historically, alternative, more rapid,
Confirmation is available in just two minutes using the Thermo Scientific
efficient, and reduce the likelihood of costly errors. “The extended validation scope for the
Salmonella testing methods have required
Salmonella Latex Test, meaning laboratories
specialised enrichment broths and
can act quickly and decisively on behalf
Salmonella Precis Method means laboratories
supplements, and incubation temperatures
of their clients.
can run multiple Salmonella testing methods, including PCR, using the same enrichment
have differed from those for other forms of
procedure. When compared to the ISO
the difficulties of testing larger samples of
THE SALMONELLA PRECIS METHOD:
substances like chocolate, infant formula, and
• Uses a single, overnight enrichment
broths needed from three to one, the number
bacterial screening. This, combined with
animal feed, have presented laboratories with something of an efficiency challenge. By incorporating Buffered Peptone Water as the enrichment medium for the Salmonella Precis Method, laboratories can reduce handling and increase workflow efficiency. The same sample preparation can now be used for other procedures such as quality indicator enumeration and detection of other bacterial targets that also use BPW for enrichment. Crucially, the method has been validated for the broadest range of food matrices,
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MARCH 2021 | FOOD REVIEW
step for fast time to result • Uses a BPW enrichment enabling
reference method it cuts the number of of plates from two to one, and uses a single, widely available culture-medium supplement.
quality indicators enumeration
It frees up incubator space, is validated for
from the same broth reducing costs
a broad range of foods together with large
• Uses liquid supplementation for higher throughput at a lower cost • Uses fewer consumables, compared to traditional methods • Is effective at between 34°C and 38°C,
sample sizes for challenging food matrices, and uses a quick, reliable confirmatory step. It slots seamlessly into standard working practices to provide greater flexibility and increased efficiency.” •
reducing the need for alternativetemperature incubators • Uses widely available broths and supplements for increased confidence in supply.
Thermo Fisher www.thermofisher.com/ salmonella-testing-food
