LMS Issue 3 2016

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L A B O R ATO R Y M A R K E T I N G S PEC T R U M ISSUE 3 | 2016 | VOL.35 | NO.3

W W W. L M S M AG A Z I N E .CO. Z A

INSIDE THIS ISSUE

04

Managing a healthy and safe laboratory

06

Understanding the data and true value of a calibration certificate

12

Learning about shark lifestyles

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Contents

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Managing a healthy and safe laboratory Tim Collins, British Laboratory Managers Association, UK

EXECUTIVE DIRECTORS: GROUP COMMERCIAL DIRECTOR: John Psillos MANAGING DIRECTOR: Bridget McCarney NON EXECUTIVE DIRECTOR: Irna van Zyl

Under the Microscope

07

Continued Professional Development

Understanding the data and true value of a calibration certificate

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11

News

Learning about shark lifestyles

Unless previously agreed in writing, LMS owns all rights to all contributions, whether image or text.

SOURCES: Shutterstock, supplied images, editorial staff.

While precautions have been taken to ensure the accuracy of its contents and information given to readers, neither the editor, publisher, or its agents can accept responsibility for damages or injury which may arise therefrom.

All rights reserved. © LMS. No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means, photocopying, electronic, mechanical or otherwise without the prior written permission of the copyright owners.

29 31

Chromatography

TLC and HPTLC MS-grade plates for mass spectrometry

Special chemistries

Meeting future energy demand

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Material Science

Micro-laser particle sizer with extra-wide measuring range

Food and beverage

39

Product Review

Laboratory safety and hygiene

Merck’s sampler and swap test kits

Centrifugation

Hettich centrifuge and accessories for washing erythrocytes

36

Guy Snelling, InterCal

28

Office: cell: email: Address:

32

Optimise industrial recipes on a smaller scale with new mini freeze dryer Element Finder plug-in software Bacteria in pure water: Controlling a growing problem

41 46

Product Directory

Comprehensive list of lab products offered by suppliers

Company Directory

Comprehensive list of company contact details

Camag AG, Switzerland: www.camag.com The World Leader in Thin Layer Chromatography (HPTLC/TLC)

4

LMS Issue 3 | 2016

under the microscope

Managing a healthy and safe laboratory Tim Collins, British Laboratory Managers Association, UK

A

s a laboratory manager, health and safety matters will inevitably be very much at the forefront of your mind.

However, it is a huge topic and it’s possible to get bogged down in the mass of detail, legislation, directives and so on, and overlook the bigger picture - why it is important and what you, as a manager, can do to promote and maintain a safe and healthy workplace.

Back to basics - it isn’t just ticking the right boxes.

Fundamentally, health and safety is about people like you and me.

It matters because failure to take it seriously can affect anyone who might be injured or become unwell because of something that happens in the workplace.

People’s attitude is probably the greatest influence on whether they will suffer injury or illhealth, so raising awareness and encouraging compliance and safe practice is worthwhile.

Legal implications There are legal implications too.

The penalties of failing to comply with UK law can be severe, with prosecutions leading to hefty fines or even imprisonment.

When safety is disregarded, the impact may be personal, practical or financial. Any or all of these could adversely affect how efficiently your laboratory runs.

The effects may be short-term, with the absence of a colleague, or over an extended period, for example through long-term loss of confidence in your business or increased insurance premiums.

The Health and Safety Executive (HSE) state that: “All workers have a right to work in places where risks to their health and safety are properly controlled.”

One of the laboratory manager’s roles is to facilitate this.

Forward planning A good manager will be committed to maintaining an overview of what happens within their laboratory, to ensure that everyone works safely and does not endanger themselves (or others), and that the laboratory operates both within the obligatory legal framework and any local organisational policies and practices.

But none of this will happen without forward planning, maintaining continuous awareness of events (normal and otherwise) in your laboratory through observation and effective communication with co-workers, and assessing risks regularly, particularly when personnel, practices or protocols change.

Equally important is analysing what happened when things go wrong and, of course, rectifying problems, keeping abreast of current legislation and training requirements (including your own), maintaining appropriate records, and a raft of other tasks. You could say that, for a manager, the key to a safe and healthy workplace is first-class organisation!

delegatING tasks However, this doesn’t mean that you must take on every duty yourself - indeed you could, and should, delegate specific tasks to capable colleagues, whilst retaining responsibility for ensuring these are carried out diligently and competently.

Not only can this lessen the manager’s workload, but involving others empowers them to take responsibility, and to view health and safety in a more positive light and relevant to them.

There’s much to consider, which can seem daunting, but you don’t have to memorise everything. In addition to information you undoubtedly already have, there is a wealth of information and resources available to guide you. The Health & Safety Executive website is a good starting point, offering industry specific and more general guidance.

The UK Institution of Occupational Safety and Health (IOSH) provides excellent advice and run useful training courses, including ‘Working Safely’ and ‘Managing Safely’.

When considering Health & Safety, what might a laboratory manager need to be well-informed about?

Firstly, be clear about the difference between risks and hazards: A hazard is anything with the

potential to cause harm to you or to your colleagues.

A risk is the likelihood that a given hazard will cause harm or promote an adverse health effect.

Broadly speaking, hazards fall into three major classes: Chemical - everything from cleaning materials, to acids, alkalis and oxidising agents, to laboratory drugs (e.g. antibiotics), solvents, and compressed gases. Chemicals may be toxic, corrosive, flammable, reactive or incompatible with other chemicals, or even explosive.

Exposure to chemical hazards can occur during use or with poor handling, storage or disposal, and the potential for harm or injury can be significant if they are misused or mishandled.

Biological - causing harmful effects in humans, adversely affecting health. Hazards can include bacteria, viruses, parasites, fungi, plants or animals (genetically modified or not), medical waste, or even foodstuffs, and be toxic, infectious or allergenic. As above, poor handling, etc. presents the potential for harm, but biological hazards differ from chemical as certain hazards can pass the threat from person to person.

Physical - encompasses a wide range of hazards. These include electrical hazards, ergonomic problems (e.g. poor posture, manual-handling issues such as lifting/pushing/pulling objects, repetitive strain from equipment use), slips/trips/falls or falling objects hazards (associated with poor housekeeping), use/ handling/disposal of sharps, temperature extremes (hot and cold), excessive/continual noise, poor lighting, mechanical hazards (e.g. using robotic or moving equipment), vibration, working at heights (even if just using a stepstool to reach material stored on a high shelf). To these we can add the potential for fire, radiation hazards, and dangers from ultraviolet light or laser use.

A further hazard class is sometimes added - psychological hazards created by work-related stress or a stressful environment.

Looking at risk, the HSE states that “You are legally required to assess the risks in your workplace so that you put in place a plan to control the risks.”

You are not expected to eliminate all risk, but you are required to protect people as far as is ‘reasonably practicable’.

Evaluating the risk associated with any of the hazards above forms the starting point for your assessments, and you need to consider who might be harmed, and how. Then decide upon appropriate control measures to minimise the risk. But this isn’t enough - you must document the assessment and, crucially, ensure the control measures are put in place, and used. Finally, you will need to review your assessments periodically, and update them whenever necessary.

It is noteworthy that the risks involved with any laboratory work increase significantly with lone working. Managers should strongly discourage team members from working alone unless absolutely necessary. If unavoidable, procedures must be in place to accommodate this, e.g. a buddy system or regular ‘check-in’ times. However, procedures must both be followed and monitored.

Regarding workplace health, we must consider how to avoid work-related illness of all types.

Physical illness is important (potential biological, chemical or physical origins should be monitored and health screening carried out where necessary), but don’t overlook the importance of other workplace stressors.

Mental well-being is vital, so think about work-life balance, interactions between individuals (e.g. preventing bullying/ harassment), how tasks are planned/carried out, and consult/ involve employees about how the laboratory should operate.

Encouraging behaviour which promotes overall health (e.g. diet, activity, smoking-cessation) is another matter to consider.

This could benefit the laboratory through reducing absence and staff-turnover, and may help increase performance, employee engagement and productivity.

Finally, the laboratory manager should ensure that all staff are aware of the organisation’s policies, procedures and rules and know who to contact if they feel they need help or support.

Source: Managing a healthy and safe laboratory.

Laboratory News

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LMS Issue 3 | 2016

MERT CERTIFICATION

Part 2

Understanding the data and true value of a calibration certificate Guy Snelling, InterCal

I

n part 1 of the article we looked at the requirements of the International Organization for Standardization (ISO) 17025 standard regarding calibration certificates, by studying a sample certificate to see how these requirements have been met.

In part 2 we continue with a more detailed explanation of some of the more misunderstood points on a calibration certificate and our travels will end with a look at how a calibration certificate can be used to increase confidence in measurements and how it can save you money.

How is all this information useful?

So we have our calibration certificate and we now have a bit more of an idea of what it all means. But what do we do with it? Well there are several reasons for having an instrument calibrated in the first place, so let’s deal with them one at a time.

Traceability The measurements that you make

with your instruments are most useful if they can be related to other, similar measurements.

Thus the mass of a sample measured in one country should give the same mass when measured in another country.

This helps companies to maintain manufacturing tolerances throughout the world so that end users can have confidence in the products that they buy. This is easily achieved when the measuring instruments in each country have both been calibrated using standards that are traceable to each other.

Calibration certificates must therefore indicate this traceability, either by listing the standards used in the calibration or by a statement to the affect that the measurements are traceable. In any case, records of standards used are kept by the calibration laboratory for inspection.

Uncertainty In an ideal world, the measured mass of a sample measured in one country would be exactly the same as the measured mass

of the same sample measured in another country.

Unfortunately there are many factors that can affect a measurement and therefore the two measurements will differ by a certain amount. The factors that influence the measurements are evaluated and an uncertainty of measurement is determined.

Taking this uncertainty into account helps us to make decisions about the accuracy of our measurements and therefore, for example, the acceptance or rejection of a sample based on its specification.

One of the factors used in determining the overall uncertainty of the measurements is the uncertainty of measurement value quoted on the calibration certificate. This should be used in your own calculations for the uncertainty of any measurements that you make with the instrument.

Another value to be used is the reference made to the ‘confidence level’ that was quoted on the first page of our example certificate. This is used

Earn CPD points! in the statistical calculations of the uncertainty of measurement.

As an example, let us take some values from our example certificate for an electronic balance. When a mass piece with a value of 100.0000g was applied, the balance indicated a value of 100.0000g. This looks as if the balance has no error, however the uncertainty of measurements is given as ± 0.0003g, and the confidence level is given as 95%.

The English translation of these figures would read as follows: When I place exactly 100g on my balance, it will read somewhere between 99.9997g and 100.0003g, 95% of the time.

Only 95% of the time? What about the other 5%, you ask.

Well, if the calibration laboratory has done its calculations correctly then statistically one measurement in every 20 may fall outside of the ±0.0003g.

This is why it is important to take multiple measurements if you want accurate results.

Much literature has been written about the calculation of uncertainties of measurement,

MERT CERTIFICATION but one that is widely used is Assessment of uncertainties for measurement for calibration and testing Laboratories, by RR Cook, more affectionately known as The Cook Book.

Reference should be made to such literature for further information about the calculations of uncertainties of measurement.

Reliability When using an instrument you need to know that the readings can be trusted. Again, in an ideal world every instrument would read perfectly every time. Unfortunately the state of affairs is not so easy and readings on an instrument will vary even when measuring the same sample, and this variance will change over time.

The degree of this variance should always be within the specification of the instrument since the specification is the basis on which the instrument was initially purchased, otherwise corrections must be made.

The results on the calibration certificate will indicate the degree

of accuracy of the instrument, but only at the time and conditions of the calibration. The tendency of these results to drift can only be determined by historical data and this is why there is the necessity for regular calibration.

Previous calibration results can be graphed against more recent ones and the drift can then be determined and extrapolated (with care) to determine the current accuracy of an instrument.

Incidentally, if a system of validations is in place, whereby a few repeatable measurements are made regularly between calibrations and are monitored, then these values can be used to predict the values that the calibration laboratory will report.

If the predicted values and the calibration values agree, then a high level of confidence is placed not only on the instruments but also on the validation system.

This can often be used to extend calibration cycles and therefore to save money.

After all, if my regular validations show that the

instrument has not drifted over a two year period, then I could make a case for extending the calibration cycle and skipping the next scheduled calibration.

But, extreme care must be taken with the validations and the additional work along with the risks of inaccurate measurements caused by extending the calibration cycle may not warrant the financial savings at all.

Accuracy Of course the most important question to be asked is: How accurate are my measurements?

This can be determined by analyzing the results on the calibration certificate.

Again, the actual results given on the certificate may vary both in specific values and also in formats, but generally the value of a standard is shown and the result obtained when using the instrument to measure that standard is given. (Obviously this applies to measuring instruments. Certificates for instruments that source a value

LMS Issue 3 | 2016

7

will give the nominal value of the instrument, along with the value measured by the standard measuring instrument).

The actual error of the instrument may then have been calculated and shown or a correction value is given. (‘correction’ is the algebraic opposite of ‘error’ and by convention corrections are algebraically added to results) At some point however, it may be tiresome to correct large errors of an instrument and the instrument will need to be adjusted. It is critical to maintaining a valid history that calibration values are determined before adjustments are made, and then those same values are measured again after the adjustments.

These are often listed on certificates as ‘pre-cal’ and ‘post cal’ values or sometimes ‘as found’ and ‘as left’ values.

However they are named, the use of these values can allow the continued determination of the drift to be maintained.

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LMS Issue 3 | 2016

MERT CERTIFICATION

Of course, if an instrument becomes faulty and requires repair and re-adjustment, then ‘pre-cal’ values cannot be determined and the historical data becomes less useful.

What about recalibration?

Even though an instrument may have been supplied with a calibration certificate from the manufacturer, unless this certificate indicates its traceability, then it’s not worth very much.

It is therefore important to have new equipment suitably calibrated at the time of purchase before putting them into use. This calibration will also form part of the commissioning or qualification of an instrument, and also gives useful evidence in a case for a warranty claim if an instrument is found to be out of specification.

But then how often should the instrument be recalibrated? The ISO standard 17025 firmly puts this decision into the hands of the user. A typical calibration

certificate may carry a statement that a recalibration period must be chosen that will ensure that the accuracy of the instrument stays within predetermined limits, but again those limits are to be determined by the user.

Generally speaking though, recalibration should be performed when you no longer have confidence in the measurements made by the instruments, or can no longer prove traceability of the measurements.

Factors that may be used to determine the recalibration period, or calibration cycle, would be the amount of use, the way it is handled, and the degree of required accuracy.

Regular validation measurements can be used to monitor the drift of an instrument and to then determine its current accuracy. Using statistical analysis of calibration results and also of validation measurements, expected calibration intervals can be chosen and either shortened or extended as required.

It is therefore important

that records be maintained of calibration, validation and maintenance work, and it may also be useful to restrict the use of the instrument only to suitably qualified persons.

As mentioned earlier, ISO17025 discourages the calibration laboratories from including a ‘recalibration date’ on a certificate. ISO does however realise that such a date may be a requirement for the customer for quality system or legal purposes, and so makes allowances for it, but only under specific instruction from the customer.

It is important then to liase with the calibration laboratory about a recalibration date, and to instruct them in writing of your requirement. Most calibration laboratories have staff with many years experience with different types of instruments used under varying conditions, and their advise can usually be sought when determining calibration intervals.

Conclusions And so we come to the end

of our journey through the calibration certificate.

We have seen why calibration certificates are issued, what information appears on the certificate and why, how to use the results data to show the accuracy of your instrument, how to use the certificate to maintain calibration history and to determine drift and therefore how determine calibration cycles.

All of this information, if used correctly, adds value to your instrument - not only in the value obtained from being able to trust measurements taken by the instrument, but also an increase in the value of the instrument itself.

Should you choose to sell the instrument at some point, the inclusion of a comprehensive calibration history file into the sale may actually increase the sell price past the initial purchase price.

So the next time you balk at the prospect of spending money on calibration, rather think of it in terms of investment rather than expense.

MERT CERTIFICATION

LMS Issue 3 | 2016

9

Understanding the data and true value of a calibration certificate

Part 2:

Questions

SURNAME

INITIALS

YOUR METCERT NO. NLA-MC-C Address: Telephone:

Fax:

E-mail:

YES! I would like to receive LMS for FREE monthly.

True or false: 1

It is optional to indicate traceability on the calibration certificates.

T

F

2

One of the factors used in determining the overall uncertainty of the measurements is the uncertainty of measurement value quoted on the calibration certificate.

T

F

3

It is important to take multiple measurements if you want accurate results.

T

F

4

The results on the calibration certificate will indicate the degree of accuracy of the instrument, but only at the time and conditions of the calibration.

T

F

5

Of course the most important question to be asked is: How accurate are my measurements? The accuracy of measurements can only be determined by analysing the results on the calibration certificate.

T

F

6

If an instrument becomes faulty and requires repair and re-adjustment, ‘pre-cal’ values can still be determined.

T

F

7

It is not important to have new equipment calibrated at the time of purchase before putting them into use.

T

F

8

Recalibration should be performed when you no longer have confidence in the measurements made by the instruments, or can no longer prove traceability of the measurements.

T

F

9

Regular validation measurements are not suitable to monitor the drift of an instrument and to then determine its current accuracy.

T

F

ISO17025 encourages the calibration laboratories from including a ‘recalibration date’ on a certificate.

T

F

10

This is to state that I have participated in the CPD-approved programme and that these are my own answers.

Signature

Date

INSTRUCTIONS: To complete the questionnaire online, go to www.lmsmagazine.co.za and click on the CPD articles button.

Click on the article on the right to access the online questionnaire. Alternatively, complete the questionnaire manually and submit it via e-mail to john.woodford@newmediapub.co.za or fax it through to 0862702680. Your certificate will be send to you within 10-15 working days.

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LMS Issue 3 | 2016

NEWS | INTERNATIONAL & LOCAL

Buchi launches their most innovative rotary evaporator in Africa

G

uest speaker, Bernie Hertz from Buchi in Switzerland recently unveiled the latest and most innovative rotary evaporator, the r-300, at launch seminars held countrywide in Midrand, Cape Town and Durban. These seminars

focused on the solutions and benefits to ease daily evaporation needs.

Delegates attending were the first ones to experience the Buchi Rotavapor ® r-300 system with help of a live demonstration

by the specialists presenting at the event, and in addition, they received guidelines and advice on streamlining applications so as to achieve the ultimate evaporation efficiency. The seminars were hosted by

Delegates attending the launch were the first ones to experience the Buchi Rotavapor r-300 system. Here the art of mixology using the Rotavapor was defined and the unit was put to the test by extracting a lemon and banana essence, using an alcohol base.

Bernie Hertz from Buchi in Switzerland recently unveiled the latest and most innovative rotary evaporator at Labotec head office in Midrand

Labotec, the Buchi exclusive local agents in southern Africa.

The new Buchi r-300 is a culmination of several years of research and experience. The new technology showed how convenient and user-friendly operation can be. Fast heating baths, immediate adjustment of heating temperature and rotation speeds and traceability of all process parameters were some of the features that impressed.

For instance a new patented combi-clip for comfortable flask and vapour duct removal is making it easier to attach and remove flasks, while an app available from the apple store and google play store will assist with monitoring the rotavapor.

To ‘discover the excitement’ the art of mixology using the rotavapor was defined and the unit was put to the test by extracting a lemon and banana essence, using an alcohol base. Delegates were able to clarify any questions or sort out any analysis problems with the help of expert advice, as well as mingle with others attending while sipping on their choice of cocktails specially mixed for them.

For any further help, advice or information relating to the new Buchi r-300 rotavapors, you can contact Labotec on 011-315-5434 or email sales@labotec.co.za

Special guest Bernie Hertz (pictured on the right) from Buchi in Switzerland demonstrating and giving guidelines on streamlining applications so as to achieve the ultimate evaporation efficiency with Buchi’s latest and most innovative rotary evaporator, the r-300.

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LMS Issue 3 | 2016

NEWS | INTERNATIONAL & LOCAL

LEARNING ABOUT SHARK LIFESTYLES

T

he KwaZulu-Natal Sharks Board (KZNSB), situated north of Durban, has been using a Telstar freeze dryer supplied by Labotec to preserve biological samples used for local and collaborative research.

Their work focuses on stable isotopes, which are delivering interesting information on the presently little known food web interactions over ontogeny, the presence of retroviral DNA, and in the future, they will be looking at projects such as the shark species’ sensitivity to mercury toxicity.

Debbie Shaw from Labotec spoke to Nomfundo Nkabi, Junior Scientist at the research centre, about the exciting work happening now, as well as future planned studies involving the preserved biological samples.

Q

Tell us more about the laboratory aims and objectives at the Sharks Board where the Telstar unit is situated?

A

The Telstar freeze dryer at the KZNSB is stationed in the wet laboratory where all sharks caught in shark safety gears are measured, sampled (e.g. taking various tissue samples for a number of research projects) and kept frozen for public dissection. The KZNSB is running a long-term monitoring collaborative stable isotope project. In the past years, it preserved more than 2 000 tissue samples using an off-site freeze dryer. It is advantageous to have the machine in-house for us to meet project time frames.

We use it for preserving various biological samples requested by local and international researcher as part of collaborative research projects.

Q A

Please tell us more about the research happening in the laboratory.

Samples of shark muscle, liver and skin have been freeze-dried for stable

isotope analyses. Sharks are large predators, considered to feed at the top level of marine food webs. Although much is known regarding their feeding behaviour in coastal regions, little is known regarding their diet and food web interactions over ontogeny (developmental history within its own lifetime). In addition, limited work to date has investigated intra-population level variation in the diet and trophic position of the white shark. The stable isotopes of carbon and nitrogen provide tools to track both baseline productivity and relative trophic positions of individual animals, respectively, and can also be used to provide information on niche width.

Samples of muscle were also freeze-dried for analyses of shark retroviruses. The literature suggests that sharks are remarkably resistant to viral infections, in part because there has been little noticeable pathology observed during studies of various shark species.

However, there have been no molecular screens to detect viruses that are not causing visible disease. The KZNSB is involved in this research by making the samples available, which enables UK researchers to identify whether retroviral DNA, from either endogenous or replicating retroviruses is present in a variety of different shark species.

Q A

What interesting future projects are on the cards?

The Telstar freeze dryer will be used by two interns from early next year for various internal stable isotope projects. We will also be using it for a toxicology study, which will focus on determining the mechanisms and consequences of mercury demethylation in fish.

The samples preserved will be used, to make predictions about species sensitivity to mercury toxicity.

All sharks caught in shark safety gears (for the protection of public swimmers on the SA coastline) by the KwaZulu-Natal Sharks Board are measured, sampled, and the preserved biological samples are used for local and collaborative research. This research is delivering interesting information on the presently little known food web interactions over ontogeny as one example of their work. To facilitate project time frames for analyses, Labotec recently supplied a Telstar freezer for on-site freeze-drying of shark muscle, liver and skin.

NEWS | INTERNATIONAL & LOCAL

Lab in a needle

A

team of engineers from the US and Singapore have developed a lab in a needle device that could provide instant results to routine lab tests, accelerating treatment and diagnosis by days.

This single, self-contained medical device will be effective, for example, in quickly detecting liver toxicity, which is a common side effect of chemotherapy. This device will test toxicity in 30 minutes while current liver toxicity tests take several days due to multiple steps required before a physician interprets the test results and communicates them to the patient.

Developed jointly by Houston Methodist Research Institute, Nanyang Technological University (NTU Singapore) and the Singapore Institute of Manufacturing Technology (SIMTech), a research institute of the Agency for Science, Technology and Research (A*STAR), the invention was explained in the recent issue of the Royal Society of Chemistry’s Lab on a Chip.

Approach The investigators demonstrated that two important steps of the lab in a needle approach accurately detected liver toxicity in pre-clinical models by measuring two genetic indicators of toxicity in aspartate transaminase and alanine transaminase.

The proteins represented by these indicators are among the most sensitive and widely used

liver enzymes in all liver function tests today.

“We used the concept of lab on a chip, which compresses the entire function of a laboratory diagnostic test onto a tiny microfluidics chip, to create lab in a needle,” explained Dr Stephen Wong, Chair of the Department of Systems Medicine and Bioengineering at Houston Methodist Research Institute.

“Our goal is to integrate sample acquisition and preparation into one device, a significant challenge that has slowed the development of point-of-care testing.”

Diagnostic testing The joint research group sought to develop a new class of device to collect patient samples, prepare them for testing, evaluate toxicity, and display results in one easyto-use process, allowing doctors and patients to immediately discuss treatment options. A compact device would also make possible diagnostic testing outside of a clinical setting, such as at home or in the field.

“What we have proven is when the doctor takes a blood or liver sample, the sample can be prepared and analysed using lab on a chip methods which eliminates the need for wet laboratory work and experts,” said Joseph Chang, a professor of circuits and systems and biomedical engineering expert and Director of Virtus, based at the NTU’s School of Electrical and Electronic Engineering.

Sample preparation was

accomplished on one chip that incorporated a miniature motor and microfluidics, while amplification was performed on a second connected chip, said Prof Wong.

Elevations in the two examined gene markers of liver toxicity were then accurately detected and consistent with previously known changes, indicating that lab in a needle is an appropriate diagnostic option.

“Our next steps are to integrate the sample preparation and analysis chips into a miniaturised device. A*STAR SIMTech will tap on its manufacturing process capabilities to develop a cost effective lab in needle device that can be scaled up for mass production. This will enable the mobile technology to be expanded to test for a number of health conditions in outpatient settings or outside hospitals,” said Dr Zhiping Wang, a principal scientist in microfluidics and Director of Research Programmes at A*STAR SIMTech.

Study outcome The study outcome represents the first time that all processes involved in the lab in a needle were integrated together successfully, and represents an important step in bringing a new real-time, easy-to-use diagnostic to the clinic and the field with immediate potential to improve patient outcomes and quality of life.

Source: Houston Methodist Research Institute

High-throughput screening strategy identifies antibiotic-resistant bacteria

A

new study in which researchers rapidly screened more than 11 000 bioactive molecules for activity against an antibiotic-resistant strain of Klebsiella pneumoniae bacteria identified multiple compounds with potent antimicrobial activity.

These active compounds included two existing drugs, azidothymidine, an antiviral used to treat HIV infection, and spectinomycin, an antibacterial agent used to treat gonorrhea, as reported in ASSAY and Drug Development Technologies, a peer-reviewed journal.

Kenneth Smith and James Kirby, Beth Israel Deaconess

Medical Centre Boston (US), describe a high-throughput screening (HTS) assay they developed that is capable of detecting either the direct antibiotic activity of small molecules or their ability to act as adjunctive treatments by synergistically restoring the effectiveness of an antibiotic

drug to which bacteria have developed resistance.

“The paper by Smith and Kirby exemplifies the importance of a carefully designed strategy for HTS and follow-up characterization of active compounds,” said ASSAY and Drug Development Technologies Editor-in-Chief, Dr Andrew Napper, Associate Director, Nemours Center for Childhood Cancer Research.

“By implementing this strategy, these authors efficiently and unequivocally identified compounds with clinically important anti-bacterial activity.”

Source: Mary Ann Liebert Inc/ Genetic Engineering News

LMS Issue 3 | 2016

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LMS Issue 3 | 2016

NEWS | INTERNATIONAL & LOCAL

Beyond LIMS

I

nterSystems, a global leader in software for connected care and developer of the InterSystems TrakCare® unified healthcare information system, recently announced that it will release its next-generation laboratory business management system (LBMS) soon.

“The nature of the laboratory business is changing dramatically,” said Martin Wilkinson, Head of InterSystems’ solutions for the laboratory market. “While the pressure to deliver more test results at lower cost will only intensify, advanced information technology will enable laboratory professionals to be the drivers of change, not the victims.”

Market trends like healthcare industry consolidation are disrupting the laboratory business. Advances in automation, genomic testing, and the increased use of point-of-care testing will drive further shifts in where and when testing takes place. Nextgeneration systems will capture information at each stage of the healthcare continuum to enable unprecedented flexibility and clinical validity.

Business Management Systems “Labs will inevitably need to move from a support service to a business that helps drive better healthcare outcomes.

“The next generation of informatics solutions are designed to manage the lab as an agile, knowledge-driven business in an increasingly interconnected

world. Today’s laboratory information management systems fall short of what labs need. We are raising the bar by introducing the world’s first LBMS, which will help customers transform from a reactive testing and results service to a proactive healthcare partner,” said Wilkinson.

An LBMS will enable laboratories to work differently. Not only will they communicate lab results electronically, but they will also identify their derivation, including workflows, equipment, and personnel involved, regardless of where they are located. Connected care models and shared electronic health records will also see laboratory professionals become more directly involved in patient care. With a continuous view of the patient’s condition, lab professionals will extend

their services into hospital wards and other settings. An LBMS will provide the following new capabilities: Distributed sample processing: Samples may be collected in one location, processed at another, analysed at a third, and digitally referred to yet another location for a second opinion.

Accountability and process improvement: Complete visibility and control over the testing process - including which tests are running at a profit and which ones at a loss - will enable greater accountability and allow laboratories to continually improve processes and optimise distributed workflows and resources.

Efficiency through automation: Standard operating procedures with full auditability

will not only support distribution of workflows across multiple sites, but also drive further automation by decreasing the percentage of tests requiring manual intervention.

Patient-centric care: Support for connected care models will put patients at the centre of the testing process with a contiguous pathology patient record, integrated within the electronic health record, including all test data - including point-of-care test results.

Quality assurance: Lab professionals will extend quality assurance outside of the laboratory using asset management functionality for equipment data, with maintenance events viewed and analysed alongside patient test results.

Source: InterSystems.

Internationally recognised standard of practice

I

n light of the importance of laboratory output and the potential of harm of incorrect laboratory results, many worldwide opinion leaders in the laboratory profession felt there was a need for an internationally recognised standard of practice for medical labs.

This need was driven by the desire to elevate laboratory quality and make efficiency a priority for all laboratories.

Another important

factor was a need to harmonize laboratory performance.

With the increasing mobility of the world’s population, it has become necessary and important to assure that test results reported in one location could be replicated, in terms of medical actions taken, at another location particularly for patients with medical conditions that require on-going monitoring.

Bio-Rad Laboratories is dedicated to advancing quality control solutions worldwide:

Quality Controls Broad portfolio of independent

controls provides an unbiased assessment of methods and system performance.

Quality control data management solutions Manage and interpret your quality control results using the industry-leading Unity™ Interlaboratory Program and benefit from data generated by users worldwide.

Education Biorad offers the educational tools and resources to help improve your ability to produce reliable test results.

NEWS | INTERNATIONAL & LOCAL

LMS Issue 3 | 2016

15

Skylabs culturing your needs in microbiology

A

s one of the fastest growing premier laboratory organisations in SA, SkyLabs have developed excellent key customer relationships both locally as well as across the African continent.

Since inception, the company’s success has grown substantially and is maintained through its competitive pricing on high

quality products accompanied by world class service.

Innovative offering As a part of our extensive innovative market offering, Skylabs is proud to introduce their range of microbiology products.

Their competitive offerings include, but not limited to, dehydrated culture media, media supplements, chemicals, bio-chemicals, culture media and identification aids.

with decades of experience in developing and manufacturing dehydrated culture media and other microbiology products.

Premier supplier

BIG OR SMALL

Skylabs is a premier supplier of high quality dehydrated culture media for industrial, pharmaceutical, biotech, food safety and life science applications.

quality products Their products are well established due to the high quality and development by a team of true professionals,

The ability to supply small or large users, quickly, without sacrificing quality is a must in today’s competitive market.

Skylabs offers a complete range of microbiology products in standard pack sizes.

Contact their friendly sales team today for more info.

Tel no: 011-434-2832 Fax no: 086-670-1774 Website: www.edx.co.za

Skylabs is a premier supplier of high quality dehydrated culture media for industrial, pharmaceutical, biotech, food safety and life science applications

DISTRIBUTORS OF: 0 Scientific, Medical Laboratory Chemicals & Apparatus 0 Research Chemicals and Solvents 0 Laboratory Glassware, Consumables and Instrumentation 0 Medical Consumables and Instrumentation 0 Microbiology Consumables and Dehydrated Culture Media

Tel. + 27 11 434 2832 | sales@skylabslns.co.za | www.skylabs.edx.co.za

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LMS Issue 3 | 2016

NEWS | INTERNATIONAL & LOCAL

Thermo Scientific wins SELECTSCIENCE Awards at Pittcon 2016

T

he global community of SelectScience readers and contributors has voted and selected the Thermo Scientific Dionex Ion Chromatography portfolio as 2016 Reviewers’ Choice Award for Instrument of the Year.

In addition, the company received the 2016 Scientists’ Choice Awards for Best New Spectroscopy Product for the Thermo Scientific Q Exactive GC Orbitrap GC-MS/MS. SelectScience announced the winners at an awards ceremony at the annual Pittcon Conference and Expo at Georgia World Congress Center in Atlanta (US).

Accepting the awards was Dan Shine, senior Vice-president and President, Analytical Instruments, Thermo Fisher Scientific.

“The annual SelectScience Reviewers’ and Scientists’ Choice Awards provide an opportunity for our community of science professionals to select the products and technologies that have made the biggest impact to their research in the past year,” said Arif Butt, CEO and founder of SelectScience.

“What’s so special about these awards is that scientists who are actively using these products are the only ones who have a say in who wins the awards. I congratulate this year’s winners on this fantastic achievement.”

“Our customers are the focus of everything we do - and we aim to accelerate their innovation and enhance their productivity through the technologies and

products we bring to market,” said Shine.

“It is gratifying when our customers and the scientific community recognise the positive impact of our product portfolios. We are honored to receive these awards.”

Thermo Scientific™ Q Exactive GC™ Orbitrap GC-MS/MS The Dionex Ion chromatography (IC) systems have led the industry for nearly 40 years, with instruments and solutions that represent state-of-the-art technological advancements and patented technologies, including the newest addition to the IC portfolio, the Thermo Scientific Dionex Integrion HPIC system.

The Q Exactive GC system

is the first to combine gas chromatography and highresolution accurate-mass Orbitrap mass spectrometry for comprehensive characteriation in a single analysis. The system is designed to increase performance in compound discovery, identification and quantitation to give GC-MS users a deeper understanding of their samples

than ever before.

Improve your laboratory today with these award winning instruments from Thermo Scientific, exclusively supplied by Anatech Instrument.

For more information on the Dionex IC system and the Q Exactive GC system, please contact us on 011-462-6776, sales@ anatech.co.za

XPE wins best laboratory balance/scale 2016 award

M

icrosep is delighted to announce that the Mettler Toledo XPE three-place precision balance won the Best Laboratory Scale/Balance category in the annual Weighing Review Reader’s Choice 2016 awards.

Mettler Toledo has introduced a new range of XPE threedigit precision balances. The extraordinary stability provided by the innovative SmartPan™ weighing pan enables one to weigh-in with 1mg readability without a draft shield, even in challenging environments.

The SmartPan is optimised to minimise the disruptive effect of air drafts, delivers results up to twice as fast and improves

repeatability by up to 50%. There is an integrated drip tray and the balance is fully cleanable in just seconds.

The new XPE precision balances incorporate additional features such as the StatusLight™ on the weighing terminal indicating to the user that the balance is ready for weighing, and the digital LevelGuide™ notifies the user of the leveling status of the balance.

The optional ErgoStand™ reduces the overall balance footprint whilst providing an ergonomic alternative to the neck-strain risk associated with repetitive weighing.

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LMS Issue 3 | 2016

NEWS | INTERNATIONAL & LOCAL

Peering into tissue stiffness

R

esearchers at the University of Maryland have developed a novel spectroscopy configuration that can obtain a sample’s entire Brillouin spectrum in one shot - saving time and allowing for noninvasive biological characterisation.

At a microscopic level, every material contains spontaneous sound waves - acoustic phonons that have properties dependent on the material’s mechanical properties. When light interacts with these acoustic phonons, it scatters and acquires a frequency shift related to the material’s elastic modulus, a characteristic measure of its ability to resist deformation and stress.

Brillouin spectroscopy A technique known as Brillouin spectroscopy works by measuring this frequency shift, and has been an effective tool for noninvasively examining materials for several decades. The technique, however, necessitates scanning interferometers, which are lowthroughput and result in long data acquisition times.

To bring this method to biological samples - such as a chicken breast, or a patient’s potentially cancerous tumour - researchers at the University of Maryland and Harvard Medical School have recently developed a new virtually imaged

phased array-based Brillouin spectrometer. They recently describe their new interferometer configuration in Applied Physics Letters.

“We have been developing this new VIPA-based Brillouin spectrometer for a long time. In this paper, we added a multi-pass low-finesse Fabry-Perot interferometer, which works as a tunable and narrowband filter,” said Giuliano Scarcelli, an assistant professor in the Fischell Department of Bioengineering at the University of Maryland.

Saving time and power While past development of virtually imaged phased arraybased spectrometers allowed the researchers to collect the entire Brillouin spectrum in a single shot, saving processing time and power, the design could only interrogate transparent materials such as ocular tissue or cells because turbid media generate too much unwanted light noise for the spectrometer to handle.

By adding a triple-pass FabryPerot filter, the researchers were able to increase the spectrometer’s ability to suppress such unwanted light component by more than tenfold, allowing them to measure background-free spectra up to 100 micrometers deep within a

sample of chicken tissue.

Future work for Scarcelli and his colleagues includes improving Brillouin technology to measure spectra faster, with greater sensitivity and at higher spatial

resolution, as well as expanding characterisation to include tumours, atherosclerotic plaques and brain tissue.

Source: American Institute of Physics

Influenza season starts late

A

ccording to the National Health Laboratory Service the 2016 influenza season is due to start later this year. In the past, influenza season started during the last week of April.

During the first nine weeks of 2016, three patients were diagnosed with influenza A (H1N1) pdm09 and influenza B. During the same period, specimens from 171 patients were received from two influenza-like illness surveillance sites.

Influenza A (H3N2) was detected in one specimen from an adult patient and influenza B from a four-year-old. Both these patients had no travel history or known contact with people who had travelled to areas where influenza is currently circulating.

Between 01 January and 03 March, specimens from 520 patients with severe respiratory illness admitted at the six

pneumonia surveillance sentinel sites were tested for influenza.

Influenza B was detected in the specimens of four patients.

In 2015, the flu season started in week 16 (ending 19 April), peaked in week 23 (ending 7 June) and ended in week 37 (ending

13 September). In past years the season has started as late as the first week of July.

Influenza subtype Data from 2015 indicated that the predominant circulating influenza subtype was influenza A (H1N1) pdm09, followed by influenza A (H3N2).

The vaccine for the southern

hemisphere will contain an A/California/7/2009 (H1N1) pdm09-like virus, A/Hong Kong/4801/2014 (H3N2)-like virus and B Brisbane/60/2008-like virus.

Strains The following strains have been recommended by the World Health Organization for the trivalent IIV 2016 southern hemisphere influenza season: A A/California/7/2009 (H1N1) pdm09-like virus.

A A/Hong Kong/4801/2014 (H3N2)-like virus.

A B Brisbane/60/2008-like virus.

These recommendations include a change in the A (H3N2) and B strains when compared with the composition of the trivalent IIV used for the southern hemisphere during the 2015 season.

Source: National Health Laboratory Service

NEWS | INTERNATIONAL & LOCAL

LMS Issue 3 | 2016

19

Thermo Scientific launches iCAP RQ ICP-MS

T

he new Thermo Scientific™ iCAP™ RQ ICP-MS spectrometer, was exhibited for the first time during Pittcon 2016 at the Georgia World Congress Center (US).

It is a robust instrument designed for maximum up-time and ease of use. It features intelligent workflows, universal interference removal and automated sample handling accessories, all within a small footprint that makes it ideal for space-restricted laboratories that still require superior performance.

The Thermo Scientific iCAP RQ ICP-MS spectrometer makes use of the Qtegra Intelligent Scientific Data Solution software, which delivers all the support features essential to any busy laboratory, while containing all the flexibility needed to achieve the most challenging applications.

The ‘Get Ready’ feature of the Qtegra software takes the ICAP RQ ICP-MS from standby to ready for analysis, through a fully automated process, saving time

and ensuring consistent results.

Automated report generation and export features, including full LIMS compatibility, makes data management effortless. The Qtegra ISDS software enables compliance with regulatory requirements, including EPA and FDA regulatory compliance and audit support, data security and access control.

The Thermo Scientific iCAP RQ ICP-MS spectrometer can handle a broad variety of samples with ease and accuracy. It comfortably meets the most demanding global pharmaceutical regulations and legislations (International Council for Harmonisation guided Q3D, USP Chapters, 232,233 and 2232).

Even the most challenging matrices of environmental samples can be handled by its comprehensive interference removal for reliable multielement analysis.

The Thermo Scientific iCAP RQ ICP-MS spectrometer easily meets all the demanding requirements, such as US

Evironmental Proctection Agency 200.8.

Unmatched performance in food, environmental, drinking water and pharmaceutical analysis is made possible by coupling a Thermo Scientific metal free ion chromatography system to the ICP-MS for speciation of critical elements such as chromium, arsenic and mercury.

The powerful and compact iCAP RQ ICP-MS spectrometer

will expand your laboratory portfolio. With uniquely reliable cell mode performance and user-friendly platform software, SQ-ICP-MS analysis is easier than ever. Ideal for labs with a focus on environmental, food, water and geoscience analysis.

For more information on the Thermo Scientific iCAP RQ ICP-MS, please contact Maxime Chalmers van den Berg at 011 4626776/ 076 687 5519 or maxime@anatech.co.za

ACQUITY Arc system awarded best New Separations Product of 2015!

T

he Acquity ® Arc™ system from Waters won the Scientists’ Choice award for Best New Separations Product of 2015 during a SelectScience ceremony at the recent Pittcon Conference and Exhibition in Atlanta (US).

The Waters Acquity Arc system, is a quaternary liquid chromatograph that gives analytical laboratories running established liquid chromatography (LC) methods a clear choice for replicating or improving their separations performance.

The Acquity Arc system is specifically engineered to respond to the needs of analytical scientists for a single LC platform that can enable them to efficiently transfer, adjust or improve their methods regardless of the LC platform on which they were developed.

Scientists working with established methods have not

had an LC platform versatile enough to bridge the gap between high performance liquid chromatography (HPLC) and ultra-performance Liquid Chromatography (UHPLC)®.

With the introduction of the Acquity Arc system, and its enabling Arc Multi-flow path™ technology, scientists now have the ability to emulate the

gradient dwell volume and mixing behaviour of various LC systems.

By selecting the appropriate fluidic path, the Acquity Arc system can easily emulate a variety of HPLC systems without altering the method’s gradient table, or provide UHPLC performance with the flip of a switch.

In addition to replicating

established HPLC assays without altering the gradient table, the Acquity Arc system can enable improved chromatographic performance of methods by leveraging 2.5-2.7 micron particle column technologies, as well as support previously developed applications on 3-5 micron HPLC columns.

Waters is offering a full complement of LC detectors for the Acquity Arc system, including photodiode array, UV/Vis, fluorescence, refractive index and evaporative light scattering. The system is also fully compatible with the Acquity QDa® mass detector.

In addition, the Acquity Arc system features novel Auto-Blend Plus™ technology that enables the operator to program directly % organic and % pH gradients to significantly reduce human error and the manual labour associated with preparing buffered mobile phases. The Acquity Arc system is controlled through Waters industry-leading informatics packages of Empower ® 2 or Empower 3 software.

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LMS Issue 3 | 2016

NEWS | INTERNATIONAL & LOCAL

Does your sodium determination method comply with the South African law?

T

he salt content of food products influences the taste, but can also adversely affect health. There is a clear link between sodium intake and high blood pressure.

The World Health Organization has published data showing that blood pressure is the number one health risk factor in Africa.

Hence, food producers are forced to declare or even reduce the standard amount of salt (sodium chloride) content in foods. In SA, reduced sodium levels should be met and adhered to, as governed by law and regulations for their respective products.

As approved by the Minister of Health, Dr. A. Motsoaledi, Gazette No. 32674: Regulations Relating to the Reduction of Sodium in Certain Foodstuffs and Related Matters states: “…the following methodology for determining typical total sodium content shall apply for monitoring purposes…”

“For all foodstuff categories, except bread, suitable potentiometric method or elemental analysis with flame atomic absorption spectroscopy or inductively coupled plasma.”

It should be noted that, unlike thermometric methods available, Mettler Toledo’s

unique potentiometric titration methods fully comply with the South African law as set out in the Gazette under the selection of analysis options on applicable foodstuffs, and serves as a cost effective solution (both initial and operational) to the alternative allowable technologies.

Current methods of measuring salt content often determine only chloride levels, which can lead to inaccurate sodium measurements due to the presence of other chloride compounds, such as potassium chloride (KCl) in addition to sodium chloride (NaCl), likely to result in an over statement of sodium content.

Similarly, alternative sodium sources may also be present that may result in an under report of sodium content.

By directly measuring the harmful sodium concentrations rather than chloride levels, Mettler Toledo’s new Excellence T-series titrators and dedicated EasyPlus™ sodium analyser provides simple, fast and accurate results in food laboratories and manufacturing processes.

The EasyPlus sodium analyser eliminates the need for expensive complex instruments. It uses an integrated and dedicated sodium evaluation algorithm to deliver highly accurate and repeatable results that are comparable to those achieved using more complex analytical techniques for total sodium analysis.

The sodium ions are measured directly without the need for calibration. Simplified sample preparation further reduces operator time and the required standards and reagents are cheaper, safer and available as ready-to-use solutions.

Similarly, the new Excellence titration T-series now includes the above features. InMotion sample changers and dosing units may be added on to achieve full automation of the method. LabX software allows connectivity for data management and direct determination of a variety of other ions. Thus, sodium, potassium and chloride parameters can be determined, using a single instrument without compromise.

Source: Microsep (Pty) Ltd

Extended specs on multi-function installation tester series

C

omtest, accredited southern African partner to Fluke and global leader in handheld electronic test and measurement equipment, has announced extended specifications and new advanced features on their tried-and-tested 1650 Series Multifunction Installation Testers.

The new series verify domestic, commercial and industrial electrical installations, and ensure fixed wiring is safe and correctly installed to meet local and international IEC 60364, HD 384 standards.

The 1650 Series allows users to

measure loop impedance without tripping RCD’s, eliminating the need to bypass them. Slim reach probes keep eyes on the panel whilst the user probes hard to reach areas. With a large display and a wide viewing angle, padded neck strap, and a compact, ergonomic design, these testers are comfortable enough to use for all-day testing.

Some extended specifications Fast high current loop test (high current mode).

Advanced loop testing prevents RCDs from tripping.

Variable RCD current mode for customised settings.

Zero adapter for easy test lead compensation The PASS/FAIL indicator takes the guesswork out of RCD testing.

Insulation test voltages (1652C: 250 V, 500 V, 1000 V), (1653B/1654B: 50 V, 100 V, 250 V, 500 V, 1000 V) for a variety of applications.

Select voltage measurement between L-N, L-PE, and N-PE. Dual display gives

simultaneous readout of mains voltage and frequency.

Auto discharge allows fast and safe discharge of electrical energy in capacitive circuits.

Added safety through live circuit detection, to check and inhibit test if circuit under test is live.

For more information on Fluke’s 1650 series of multifunction installation testers, or info about seminars, demos or to locate the nearest dealer, contact Comtest on 010 595 1821 or sales@comtest.co.za

NEWS | INTERNATIONAL & LOCAL

LMS Issue 3 | 2016

21

Preventing an environmental disaster

O

ptical emission spectrometers can contribute decisively to prevent accidents. Bruker Elemental’s Q4 MOBILE provides the petrochemical and metals industries with a compact metal analyser, capable of analysing the critical carbon content in used steel. The smallest concentrations of carbon in the ppm range impact the steel’s durability, strength and weldability.

In April 2010, seven employees were killed in an explosion caused by a high-temperature hydrogen attack in a refinery in the US state of Washington. Since the accident was caused by metal fatigue, mobile OES instruments were needed.

Since then, relevant equipment, especially process piping systems have to be tested in accordance with the ‘Material Verification Program’ (guidelines API 578) of the American Petroleum Institute.

Components that do not meet the required specifications are identified and replaced.

For this task Bruker Elemental´s Q4 MOBILE is the optimal control and measurement analyser.

With the last two years very successfully on the market, the Q4 MOBILE performs rapid metal analysis, identification and sorting. The highlight is its mobility.

With an optional four-wheeled trolley, the spark spectrometer and accessories easily roll to the place where analysis is needed.

Controlled by an intelligent energy management system, Q4 MOBILE can use any standard power source, even a cigarette lighter in a car.

Adaptive Ambient Control (AAC), which adjusts the optics automatically to the ambient temperature, ensures a short preparation time.

Long setup times are a thing of the past. Work is carried out with the help of a handy probe, which allows ergonomic handling thanks to the lightweight, flexible hybrid cable, even during prolonged activity.

Depending on the requirement, whether in spark or arc excitation, the quick change adapter allows switching the analysis mode in seconds.

For shorter wavelengths such as UV, a rapidly modifiable probe with high resolution optics is available. Thus even the most demanding applications, such

as C, S or N determination, are possible. This underlines strongly the outstanding Bruker analytics of the Q4 MOBILE spectrometer.

“For 316 and 316L stainless steel alloys, the difference in the carbon concentration is indeed minimal, but this difference is of decisive influence on the respective corrosion behaviour,” said Bruker Product Manager, Hans Dominick.

“Our Q4 MOBILE analyses carbon contents in the corresponding ppm range

and can thus clearly determine the respective alloy. The use of components with incorrect element composition is prevented and expensive failures or dangerous accidents avoided.”

Via a display on the probe, the analysed alloy designation is directly shown. Nothing could be faster. High capability, practical relevance, ease of use and functional mobility - these are the strengths of the Q4 MOBILE. Thus Bruker fulfils all customer requirements.

“Not only the petrochemical industry appreciates the Q4 MOBILE in the implementation of current guidelines for material control and material testing on oil rigs or pipelines.

The Positive Material Identification in incoming goods inspection or metal sorting is another important use for material traders, in which rapid analysis brings clarity and ensures functional processes. Downtime costs a lot of money, and this can be avoided with Q4 MOBILE..

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LMS Issue 3 | 2016

NEWS | INTERNATIONAL & LOCAL

First successful extraction of ancient DNA

R

esearchers have presented one of the first computerised tomography (CT) scans of a mummified individual from southern Africa, and also completed the first successful aDNA (ancient DNA) extraction from such remains. The mummy is estimated to be about 300 years old.

Prof Maryna Steyn, Head of the School of Anatomical Sciences at the University of the Witwatersrand, together with researchers from the University of Zurich (Switzerland), University of Pretoria and University of Botswana have published these findings in a paper published in the South African Journal of Science.

Mummified human remains are valuable sources of information on past populations. The researchers reported on the radiological and molecular findings of a partially mummified individual found in northern Botswana, in the Tuli block.

The researchers noted that this desiccated mummy from the Tuli

The intact mummy, covered with animal skin, discovered in the Tuli region of Botswana.

Food allergies better understood

S

cientists have taken a step closer to explaining food allergies, especially those in children.

The new research shows how food antigens stimulate immune cells in the gut that prevent the immune system rejecting food.

“The immune system evolved to protect us from things that are not ourselves, like viruses or pathogens, yet we consume nutrients, which are themselves foreign.

“Our work shows food tolerance is acquired and involves specific populations of T cells that develop following its consumption. Without them, we would mount a strong immune response to macromolecules contained in food,” said Dr Charles Surh of the La Jolla Institute in California (US).

Scientists used antigen-free mice raised in a germ-free environment and only fed amino acids were depleted of T-regulatory (Tregs) cells.

However their counterparts who were fed a ‘normal’ protein diet had a large number of these cells. Tregs cells control the immune system, and prevent autoimmune disease.

This study is the first to demonstrate the effect a normal diet has on gut cells that suppress rejection of food by the immune system.

Researchers believe this can explain why children are more

susceptible to food allergies as they have more limited exposure to novel foodstuffs.

As well as demonstrating this, researchers looked into what happens when immune cells ignore harmless antigens.

Antigen-free mice were exposed to ovalbumin (a protein) and a massive immune reaction was observed.

The researchers proposed this occurred as the antigen-free mice did not have a population of immunosuppressive Tregs that would usually reduce the inflammatory response to food.

“We are now examining the cellular and molecular details of how the ‘default’ strong T cell response to food is regulated, in this context, we plan to pay particular attention to certain foods, such as peanut, egg and other foods that cause food allergy,” said Surh.

Source: LabNews, 2016.

region is the first to have been reported from this region. The remains were those of an older male adult of African origin. He was interred in a tightly flexed position and wrapped in an animal skin (and tied together with rope).

CT scanning revealed that none of the internal organs were preserved. Multiple post-mortem alterations are seen, but apart from some degenerative changes of the lower vertebral column, the axial skeleton has remained intact.

The advanced osteophytosis (degenerative changes of the spine) suggests an older age than what was previously estimated.

The cause of death could not be confirmed, but he did not shown any signs of injury.

The aDNA analysis confirms that the individual possibly had a Sotho-Tswana or Khoesan genetic relatedness, as could be expected from individuals from that region.

For more information, please email Prof Steyn: maryna.steyn@wits.ac.za Source: University of the Witwatersrand.

Traditional skin tests ineffective

S

kin tests traditionally used to predict allergies to amoxicillin, one of the most commonly prescribed antibiotics in children, are ineffective, according to a new study led by a team at the Research Institute of the McGill University Health Centre (RI-MUHC) in Montreal (Canada).

“Our study suggests that skin tests are essentially useless as diagnostic tests,” said lead author Dr Moshe Ben-Shoshan, an allergist at the Montreal Children’s Hospital.

The results are a game changer in the way physicians assess amoxicillin allergy in children given the fact that skin tests are still the recommended screening method in hospitals, he added.

“Our study is the first to determine the percentage of immediate and non-immediate amoxicillin allergy in all children presenting with a suspected amoxicillin induced rash through a graded provocative challenge,” said Dr Ben-Shoshan.

Source: McGill University Health Centre

NEWS | INTERNATIONAL & LOCAL

LMS Issue 3 | 2016

23

Wirsam’s XRF training a great success!

F

rom 7-11 March 2016, delegates from around SA attended a week-long X-ray Fluorescence (XRF) Practical Training Course hosted by Wirsam Scientific at their head-office in Johannesburg.

The presentations were led

by Prof (Emeritus) James Willis, Dr Clive Feather, Marius de Jager, George van der Walt and Benjamin Marrian.

The course covered many aspects including radiation safety, x-ray physics, sample preparation, wavelength dispersive x-ray fluorescence spectrometer (WDXRF), energy dispersive XRF Spectrometer (EDXRF), XRF quality control, fault finding tools, trouble shooting and XRF maintenance. There were ample opportunities for discussion and question and answer time.

Although the course was instrument neutral, the course hands on demonstrations were held at Wirsam Scientifics’ state of the art Analytical X-Ray

Delegates attended a week-long x-ray fluorescence practial training course hosted by Wirsam Laboratory facilities, which demonstrated the capabilities of many of the instruments including: Rigaku NEX CG EDXRF, Rigaku Supermini 200 WDXRF, Katanax fusion machine, Spex sample prep pellet press, CKIC mills and analytical balances.

This Analytical X-Ray laboratory facility is revolutionising the way in which we teach x-ray and sample preparation and look forward to teaching many generations of scientists in this state of the art laboratory.

Should you be interested in finding out more about our upcoming x-ray and sample preparation courses please contact Mariana Wirsam mariana@wirsam.com to keep you updated on the upcoming dates.

Trina Solar named world’s most bankable PV module manufacturer

T

rina Solar Limited, a global leader in photovoltaic (PV) modules, solutions, and services, recently announced that is has been named as the most ‘bankable’ PV module manufacturer globally by Bloomberg New Energy Finance (BNEF).

In its survey of key PV stakeholders asked about 50 different module brands, 100% of respondents said that Trina Solar was bankable, confirming the company’s leading position in the solar PV market.

Trina Solar topped the list of 50 module manufacturers, being the only company that all survey respondents believed

was bankable. The report also details how Trina Solar modules were used in more debt financed projects than any other manufacturer›s modules since the start of 2014, having secured funding for more than 1.2GW for 15 different projects tracked by the BNEF database. The BNEF database is not fully comprehensive, but contains almost 14 000 solar financings worldwide.

Commenting on the report findings, Teresa Tan, Chief Financial Officer of Trina Solar, said: “The findings from this report are highly positive and we believe that they come as further confirmation of our leading

position in the industry around the world.

“Trina Solar prides itself on the sustainability and strength of its way of doing business and this latest acknowledgement of our leading bankability comes as a logical result. We always strive to deliver bankable PV solutions

that are innovative and that will provide the best returns for our customers. We have invested heavily, and will continue to invest, in the quality and performance of our PV modules to ensure that they can deliver the highest yields in the short and long term.”

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LMS Issue 3 | 2016

NEWS | INTERNATIONAL & LOCAL

Zika virus: A global concern Reported forms of transmission

Accidental laboratory exposure Blood transfusion

Rhesus monkeys (R)

ZIKV: Saliva (19.2%) ZIKV: Blood Sexual (8.8%) ZIKV: Serum

Experimental inoculation?

Experimental inoculation?

Rhesus monkeys (ZIKV, 1947)

Swiss albino mice (S) Cotton rats (R) Guinea pigs (R) Rabbits (R)

T

he World Health Organization (WHO) has warned that the Zika virus is ‘spreading explosively’ in the Americas and the region may see up to four million cases of the disease strongly suspected of causing birth defects.

In February this year, the first case was reported in SA. According to the WHO, 64 countries and territories have reported transmission of Zika virus since 1 January 2007.

Zoonotic pathogen Zika virus is a zoonotic pathogen, naturally and experimentally hosted in non-human primates as well as experimentally in Swiss albino mice. Rhesus monkeys can be the source in natural habitats of human infections, through the bite of Aedes aegypti and A. albopictus (multiple other species and genus have been implicated), in infected monkeys and later transmission to susceptible human hosts.

ZIKV: Semen (up to >2 months)

Organ transplantation

Main vector: Ae.aegypti Other vectors involved: Ae. africanus (ZIKV,1948) Ae. furcifer Ae. taylori Ae. lucteocephalus Ae. dolzieli Ae. opok An. coustani Mansonia uniformis Culex perfuscus Others

ZIKV: Urine (until 14 days)

Transplacental

Perinatal Monkey bite

Risk of associated microcephaly

Vector-borne

Non-vector-borne (S): Susceptible, develops clinical disease; (R): Resistant to clinical disease

Vector-borne disease Zika virus is predominantly a vector-borne disease, although after the epidemics in the Pacific region it was clear that transplacental and perinatal transmission could also occur.

However, the associated risk of microcephaly has been identified and highlighted much more recently as evidences continue to be added in different studies.

Before the current epidemics not a single study raised that relationship, and, right now a significant number of pregnant women and their newborns are being monitored in Brazil, Colombia and other countries in the region for central nervous system anomalies.

Zika has been detected in newborns, placenta and umbilical cords, as well in pregnant women by reverse transcription polymerase chain reaction RT-PCR. There have been no reports detecting viable and potentially infective virus in

breast milk up to now.

Mother-to-child transmission In addition to mother-to-child transmission, during the last decade, cases of sexual transmission have been reported, representing a nonvector borne form of transmission of Zika virus. Zika virus has been detected in human saliva, blood, semen and urine.

It has been recently detected in semen and urine of, respectively, a patient after 62 days of infection and another patient after 14 days of infection. The spread of the virus through blood transfusion and organ transplantation have been reported or suspected. Zika virus infections have been documented through laboratory exposure.

Another emerging aspect of this zoonosis has been the possible transmission through bites of monkeys and other non-human primates. This has also been recently reported.

Conclusion Zika virus is primarily a vectorborne disease (mainly by A. aegypti), but there are also secondary modes of transmission (mother-to-child, sexual, blood transfusion, transplantation, nonhuman primate bites).

This imply that prevention and control should consider all these ways of transmission, providing strategies to reduce new infections from this arbovirus that still need further basic, epidemiological and clinical assessment in order to clarify and understand its real impact on human health. Zika represents a real challenge for the medical and scientific community.

Reference Rodriguez-Morales AJ, Bandeira AC, Franco-Paredes C. The expanding spectrum of modes of transmission of Zika virus: A global concern.

Annals of Clinical Microbiology and Antimicrobials, 2016.

Mimicking evolution

P

roteins are nature’s machines.

They provide oxygen to power our muscles, catalyse reactions that help us extract energy from food, and fend off infections from bacteria and viruses.

For decades, scientists have searched for ways to design new proteins that can serve specific purposes in medicine, research, and industry. Now, researchers at the University of North Carolina School of Medicine have

developed a method that creates novel proteins by stitching together pieces of already existing proteins.

The technique, called SEWING, is inspired by natural evolutionary mechanisms that also recombine portions of known proteins to produce new structures and functions. This approach can generate a diverse set of protein structures with many of the distinctive features that proteins require to carry out specific

biological functions.

The findings, published recently in the journal Science, could enable researchers to design proteins to play a variety of different roles in human biology and disease, such roles as catalysts, biosensors, and therapeutics.

“We can now begin to think about engineering proteins to do things that nothing else is capable of doing,” said senior study author, Prof Brian Kuhlman of the

UNC Lineberger Comprehensive Cancer Center.

“The structure of a protein determines its function, so if we are going to learn how to design new functions, we have to learn how to design new structures.

Our study is a critical step in that direction and provides tools for creating proteins that haven’t been seen before in nature.”

Source: University of North Carolina Health Care.

26

LMS Issue 3 | 2016

NEWS | INTERNATIONAL & LOCAL

Transforming water and carbon dioxide into energy

F

or years, scientists have been trying to emulate photosynthesis, the process by which plants, algae, and some bacteria harness light from the sun to chemically transform water and carbon dioxide into energy that is stored for later use.

An artificial version of photosynthesis could provide a clean, renewable source of energy to help satisfy society’s growing demands. For artificial photosynthesis to become a viable alternative to fossil fuels, the efficiency and speed of water oxidation - the reaction that turns water into oxygen gas, hydrogen ions, and electrons - is one of the processes that must be improved.

Now, a team of scientists from the US Department of Energy’s Brookhaven National Laboratory, Adam Mickiewicz University, and Baruch College, City University of New York, has synthesised two new molecular catalysts for water oxidation.

The catalysts - complexes of ruthenium surrounded by binding molecules (ligands) containing phosphonate groups - accelerate the formation of the oxygen-oxygen bond, usually the most energyintensive and slowest step of water oxidation. Initial studies, described in a paper published on May 11 in Angewandte Chemie International Edition, demonstrated that these ruthenium complexes could offer a low-energy pathway to faster water oxidation.

Storing solar energy “Storing solar energy as hydrogen fuel or carbon-based fuels like methanol requires catalysts that can oxidise water at fast rates, with high efficiency, and

for long periods of time,” said Javier Concepcion, an author of the paper and a chemist in the artificial photosynthesis group at Brookhaven Lab. “Our ruthenium complexes catalyse the oxygen-oxygen bond formation faster than any other known catalysts, generating hundreds of oxygen molecules per molecule of catalyst per second.

With these catalysts, the electrical potential required to start the reaction is approximately 10 times less than that of a AA battery.”

Forming the oxygenoxygen bond In water oxidation, four protons and four electrons - required in a subsequent reaction to convert carbon dioxide into usable energy - are removed from two water molecules, and an oxygenoxygen bond is formed. For water oxidation to occur, the bonds between hydrogen and oxygen atoms in the two water molecules must be broken. In the case of artificial photosynthesis, a chemical catalyst triggers this molecular breakup.

“Water is a very stable molecule, so getting two water molecules to react with each other is very difficult,” explained first author Yan Xie, a doctoral candidate at Stony Brook University and a research assistant in Brookhaven’s artificial photosynthesis group. “Our ruthenium complexes provide the reactivity needed to break those bonds.”

The paper describes details of the series of steps through which the catalyst initiates and completes the reaction. In short, one of the water molecules binds to the ruthenium complex and loses protons as the complex is oxidized (loses electrons), resulting in an

electron-deficient ruthenium-oxo group. Then, with the assistance of a phosphonate group, the other water molecule reacts with this highly reactive ruthenium-oxo to release molecular oxygen (O2).

“The phosphonate group accepts protons, or hydrogen ions, from water,” said coauthor David Shaffer, a research associate in Brookhaven’s Chemistry Department. “It is positioned near the active site of the ruthenium complex where water oxidation occurs. Incorporating the phosphonate group and ruthenium in a single complex makes it easy for the water molecule to find that one site and react.” Eventually, the protons are transferred from the phosphonate group to the surrounding solution.

Studying the electrochemistry of the ruthenium complexes To determine the efficiency and rate of water oxidation with the ruthenium catalysts, the team studied the electrochemistry of each oxidation state by applying different voltages and measuring the amount of current flowing through the system at various pH values (the concentration of protons in the solution).

“The voltage at which catalysis starts tells you about the energy efficiency of water oxidation, while the current tells you how quickly water oxidation is occurring,” explained Concepcion. “Our ruthenium complexes minimise the amount of energy lost as heat, both in terms of the voltage and the rate that would be required for the catalyst, if incorporated into a device, to make use of all incoming sunlight.”

The team also used computational modeling to study

the activation parameters - the energy and molecular order required to break and make bonds during the key reaction between the water molecule and the ruthenium-oxo group.

The computational studies showed why the phosphonate group resulted in faster catalysis.

“Phosphonate is a good proton acceptor, so it energetically favors the reaction. Because it is part of the ligand, it is already positioned and ready to interact with water, removing the need for a more ordered arrangement of molecules,” said Concepcion.

From separate studies, the scientists were able to tell that one of the oxidation steps not the oxygen-oxygen bond formation step - was limiting the rate of the catalysis. The team is now developing secondgeneration catalysts to optimise this step.

Eventually, they hope to make equally reactive catalysts using metals such as iron and cobalt that are more abundant and less expensive than ruthenium, but whose chemistries are much more complicated.

“By incorporating these catalysts into systems capable of absorbing sunlight and combining them with catalysts that reduce carbon dioxide or water into fuels, artificial photosynthesis could become a practical approach for storing solar energy as fuels,” said Concepcion.

Source: DOE/Brookhaven National Laboratory. Speeding up key oxygen-oxygen bondformation step in water oxidation: New molecular catalysts could drive reaction needed to efficiently store solar energy in chemical bonds of clean fuels.

ScienceDaily, 2016.

NEWS | INTERNATIONAL & LOCAL

LMS Issue 3 | 2016

27

SA ranks low on global innovation list

S

outh Africa ranks 55th in how its domestic policies support worldwide life sciences innovation, according to an analysis released recently by the Information Technology and Innovation Foundation (ITIF), a global technology policy think tank.

The report assessed 56 countries, which together comprise close to 90% of the world’s economy, on the extent to which their scientific research, drug pricing, and intellectual property policies contribute to global biopharmaceutical innovation.

“Life sciences innovation requires years of painstaking and expensive research. To ensure global health outcomes continue improving, more nations must do their share to support biopharmaceutical innovation and not free ride off the hard work and investment of the leaders, said coauthor Stephen Ezell, ITIF Vice-President for

Global Innovation Policy.

Innovation Building on previous ITIF research that studied the impact of national policies on the global innovation ecosystem, this report shines a light specifically on biopharmaceutical innovation.

Ezell and coauthor John Wu examined three policy areas that not only support life-sciences innovation domestically but also have positive spillover effects globally: Governments’ research and development expenditures on health, the extent of price controls on biopharmaceutical drugs and intellectual property protections for life-science innovations.

The analysis found that the US, Switzerland, Taiwan, Singapore, and Sweden have enacted policies that, on a per-gross domestic product basis, contribute the most to

global life-sciences innovation, while India, SA, Thailand, the Philippines, and Australia have policies that contribute the least.

“Despite tremendous progress over the past half century, the world is still not producing as much life-sciences innovation as is needed or possible,” said Ezell.

“Countries that fail to invest adequately in life-sciences research, pay less than their fair share for drugs, or put in place weak intellectual property protections for drugs hurt the entire global community by slowing down biopharmaceutical innovation that could cure or better manage diseases for future generations.”

Ecosystem The report also found that countries with the strongest life-sciences policies also have some of the most competitive innovation ecosystems, meaning

that doing well domestically can also mean doing well for the world.

“It is on the one hand understandable that policymakers tend to focus first and foremost on the short-term interests of their own citizens, but too many ignore the fact that this comes at the expense of less innovation of new drugs,” said Robert Atkinson, ITIF president.

“The bottom line is that all nations need to do their part to support robust global biopharma innovation.”

How four-stranded DNA is formed and unfolded

R

esearchers at Umeå University in Sweden have discovered that specific DNA sequences that are rich in the DNA building block guanine in the yeast species, Schizosaccharomyces pombe, can form four-stranded DNA. The researchers also show that the motor protein Pfh1 can unfold these DNA structures and thus contribute to maintaining an intact genome. DNA molecules are known for their spiral shape, with two strands wrapping around each other.

But DNA can also appear in

other configurations. One of these forms consists of a guanine-rich four-stranded DNA, a so-called G4 structure. Guanine is one of DNA’s four building blocks.

A number of earlier studies of different organisms have indicated that G4 structures are formed in the genome and fulfil important biological functions such as controlling gene expression.

G4 structures can present a threat if they exist continuously in the genome. Helicases are a type of specialised motor protein with the ability to unfold different kinds of DNA.

Until now, there were very few studies about G4 structures in Schizosaccharomyces pombe, also known as fission yeast. It was also not known which protein is responsible for the unfolding of the G4 structures.

Research on G4 structures is at its early stage. But researchers believe that G4 structures can inhibit certain processes in the cells, including DNA replication, and have tied them to the development of cancer and neurodegenerative diseases in humans.

Source: Umea University

28

LMS Issue 3 | 2016

Laboratory safety and hygiene

Merck’s samplers and swab test kits

M

erck Millipore’s samplers

that are growing on your sample

routine microbiological analysis of liquids and surfaces for bioburden levels of bacteria, yeast and moulds. This technique eliminates

Liquid monitoring using Samplers A sampler consists of two parts: A plastic dip test handle with a

the cumbersome and expensive

0,45µm black gridded Millipore

hardware and materials associated

filter and an absorbent pad. This

with traditional testing methods.

part contains the dehydrated

The testing is as simple as:

1 2 3

Sample: Obtain a sample.

Samplers are filled to

hydrates the agar medium which provides nutrients to

and swab test kits simplify

nutrient medium for recovery of specific organisms.

An outer plastic sheath. This

the top line. Swabs are

piece is filled with the sample

wiped across a surface.

liquid of your choice.

Incubate: Place in an incubator

When the sampler is immersed in

the organisms on the filter.

These organisms will grow into defined colonies, which can be examined or counted.

Surface monitoring using swab test kits Swab test kits combine a

difficult areas where bacteria, yeast or mould can grow. The cap design is easy to use and safe allowing the operator to hold the

Sampler with a Swab. A Swab

paddle without contaminating

consists of two parts:

sterile surfaces.

A plastic cap connected to a polyester swab An outer sheath containing

(48 to 72 hours) or incubate

a liquid, the absorbent pad rapidly

pre-measured sterile

at room temperature for 7

absorbs 1 ml into the filter membrane.

phosphate buffer solution.

days depending on the test.

Bacteria, yeast or mould larger than

The swab enables you to

Count: Identify colonies of

the rated pore size are retained

monitor surface cleanliness, test

bacteria, yeast and/or mould

on the filter surface. The liquid

machine surfaces, and reach

Merck Pty Ltd Tel: 08600 63725 Fax: 0860 522329 Email: labsupply@merckgroup.com

On the spot hygiene detection Perfect cleaning solution

R

ecognised as a superior method

sampling device, test chamber

of hygiene monitoring in the

and reagent-dispenser all in one.

food and beverage industries,

There is an integral buffer solution

E

xtran® laboratory cleansers are the perfect solution for cleaning

that ensures results are unaffected

to Millipore’s

applications including paints, biocide

by sample conditions. The pen has a

well-known

efficacy, waste water and jet fuel.

unique built-in inhibition protection.

quality standards.

The HY-LiTE 2 swab testing allows

you time and money.

Extran is free from

your laboratory utensils

now also available for industrial

The HY-LiTE® 2 provides a crucial,

cleaning validation, saving

Depending

scents and dyestuffs and does not contain chlorine or other toxic ingredients: It thus prevents odours,

accurate, instant and comparative

better in-depth and aggressive

on the type of

data on the efficiency of cleaning

investigation of surface hygiene

contamination

regimes and personnel. The results

than traditional microbiology

and the material

Our laboratory cleansers are also

are not affected by temperature

methods, e.g. contact plates.

to be cleaned, the

free of silicones and oxidants.

change. It also alerts management

The HY-LiTE 2 hygiene tracking

Extran range of

protecting the health of the laboratory staff in the process.

The active ingredients in Extran are biodegradable: Extran

to potential problem areas that

system works by detecting

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might need special attention -

adenosine triphosphate (ATP), a

the ideal solution

immediately and over the long term.

substance found in all living cells

for the cleaning

controlled production conditions

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environmental protection. In almost

Unlike traditional microbiology, HY-LiTE 2 can detect biological residues on surfaces which have just been rendered sterile by chemical

Unlike traditional microbiological methods, which detect the presence of bacteria and other microorganisms, ATP detection

disinfection but still

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on superficially sterilised surfaces,

HY-LiTE 2 provides definitive test results in

potentially

just 60 seconds. A single

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microbial

might

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help to spell the difference

The sampling pen is a preprepared,

rinse water control. Containing all the reagents necessary in the correct amounts, each pen is a combined

chromosulphuric acid, which is still

Millipore and use our detergents

common on the market, unnecessary.

for manual cleaning or machine

It is thus gentle on the environment

cleaning in laboratory washing

and on the health of staff.

machines. Both processes generally require different detergents.

Extran as an all-purpose cleaner: Extran was developed especially for use in laboratories, but can also

Your advantages Extran cleans reliably, leaving

be used in production facilities.

The consistent composition

no residues: This prevents

means that processes and

residues from being transferred

applications do not have to be

into the next analysis or test.

constantly adjusted.

Extran: Millipore provides a practical

run and a contaminated one.

formats - for surface testing - and for

all cases, Extran makes the use of

of Extran experience from

Residue-free cleaning with

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prove the freedom from residues of nonionic surfactants after the cleaning process by means of a photometric test.This helps you in preparing your own individual

Merck Pty Ltd Field Marketing Manager: Lennet Maduray Tel: +27-11-372 5148 Email: lennet.maduray@merckgroup.com

Chromatography & HPLC

LMS Issue 3 | 2016

29

Lipstick and chromatography?

S

cientists from Western Illinois

scene - fingerprints, bullet cases and

found in the sample with an organic

University (US) have developed

lipstick stains - which can apparently

solvent before using a different more

a technique which could help

be analysed in minutes, providing

basic solvent to extract whatever

police in the search for crime

valuable leads to the investigation.

is left. In this way, they can quickly

suspects. Although the method

The reality is that it’s far more

and effectively prepare the sample

is in the early stage, the team

time-consuming and expensive than

without losing any of the evidence.

hope that they will soon be able

depicted in forensic crime series.

to take samples from any crime

‘Working on this investigation

After securing the sample, Esterlen and her team then worked

scene - a mug, a shirt collar or other

has opened my eyes to the fact

on determining which was the

incriminating spot - then use gas

that TV has it wrong - things

best approach to analysing it. After

chromatography to analyse it.

take much longer in real life,’

testing both high performance liquid

explained Bethany Esterlen, lead

chromatography and thin layer

researcher on the project.

chromatography, they concluded

In the past a lipstick stain at a crime scene could only give limited information about the cosmetics

Over the years, scientists have

that gas chromatography was

the suspect may have worn - unless

developed a number of complex ways

the most effective technique of

the police resorted to expensive

to analyse evidence such as lipstick

determining the specific chemical

and complex techniques.

stains. Some of these techniques

makeup of the cosmetic.

This new method uses simple techniques and will let the police know the exact brand and style

involve expensive instruments and need specially trained staff.

Esterlen and her team worked on

The researchers tested out 40

crime scene - if a lipstick sample is found. By checking whether or not the

different lipsticks using the technique

suspect uses that particular brand,

and have begun to compile a database

the prosecution might just have

in a cheaper and more timely

a method to lift the lipstick samples,

of the exact chemical makeups of

another piece of evidence in the case.

manner - helping them to quickly

which simplified the process and

known brands. As their lipstick library

Source: How to Catch Criminals

get a profile of the suspect.

eliminated unnecessary steps. In

grows, police should theoretically be

by Their Choice of Lipstick with

their final process, they remove

able to determine exactly which kind

Chromatography. Chromatography

the majority of the oils and waxes

of lipstick was used by a suspect at a

Today, 2016.

Fans of shows like CSI will be familiar with tell-tale signs at a crime

30

LMS Issue 3 | 2016

Chromatography & HPLC

TLC and HPTLC MS-grade plates for mass spectrometry

S

CAMAG interface).

has been developed, thus

imple thin layer chromatography

the silica and transferred to the

(TLC) is the most widely used

MS in the gas phase. Vaporisation

Both approaches are offline,

providing high efficiency.

technique in planar chromatography,

techniques include gas beam, ion

and both are performed after the

One particular advantage of

whereas high performance TLC

bombardment and matrix assisted

separation is finished and the plate

TLC-MS and HPTLC-MS is the

(HPTLC) is considered to be the most

laser desorption/ionisation or

is dried. The sample transfer to

flexibility in choosing mobile

efficient and powerful technique. In

direct analysis in real time.

the mass spectra (MS) is fast and

phases for a separation. By

HPTLC, the silica used has a smaller

contrast, with standard LC-MS

particle size (4µm - 8µm) and a

B

typically takes less than one minute.

narrower particle distribution.

dissolved in a solvent and transferred

Key benefits of TLC-MS are:

mobile phases cannot be used

to the mass spectrometer in the

Mass spectra are obtained quickly

(e.g. inorganic buffers).

The techniques The techniques for coupling

With elution-based techniques,

liquid

by direct sample access on the

phase (see

TLC plate at room temperature high quality spectra are obtained

TLC with mass spectrometry

with low background signal.

can be divided into:

A

coupling using HPLC, some

the analyte on the silica plate is

With desorption-

Targeted recording of mass

based techniques,

spectra on zones or lines of

the analyte is

interest is performed after

vaporised from

the TLC chromatogram

Merck Pty Ltd Field Marketing Manager: Lennet Maduray Tel: +27-11-372 5148 Email: lennet.maduray@merckgroup.com

Waters Cortecs Solid-Core LC columns

W

aters has added to its Cortecs® columns product

Capable of operating at very

transfer or scale compendial

high column efficiencies and low

C8 HPLC methods to a more

family with the introduction

backpressures, the new columns

robust column technology.

of two new chemistries.

give scientists additional selectivity

Cortecs phenyl columns

choices and allow them to develop

are an excellent alternative

core particle technology, the

methods quickly, and generate more

to typically bonded C18 phases,

Waters® Cortecs C8 and Cortecs

information with every analytical

particularly for aromatic

phenyl analytical columns are

run. The Cortecs C8 and Cortecs

compounds, based on the unique

designed for scientists who need

phenyl chemistries are available

selectivity provided by the phenyl

in Waters current Cortecs

to expand their chromatographic

in two particle sizes, 1.6 and 2.7

bonded phase. Both the 1.6 and 2.7

product portfolio.

separation space while maximising

micron, and are offered in a total of

micron Cortecs C8 and Cortecs

the speed, resolution and sensitivity

50 unique column configurations.

phenyl columns are fully scalable for

Built on Waters proven solid-

of their small molecule for high-

Cortecs C8 columns exhibit a lower

the seamless transfer of methods

performance liquid chromatography

hydrophobicity when compared

between the two particle sizes.

(HPLC), ultra high performance

to typical bonded C18 phases and

The two new chemistries

liquid chromatography (UHPLC)

are useful for separating strongly

complement the existing Cortecs

or ultra performance liquid

hydrophobic compounds. They are

C18+, C18, and hydrophilic interaction

chromatography (UPLC®) separations.

also ideal for chemists looking to

liquid chromatography chemistries

Microsep Contact: Louis van Huyssteen Tel: 011-553-2300 Email: louis.vanhuyssteen@microsep.co.za

Straightforward determination of fluorine in copper concentrates offers a novel and robust method that is ideal for these types of samples.

CIC is a hyphenated technique

CIC as enabled by the Methrohm CIC is straightforward to use, making this method highly suitable for

combining sample digestion by

routine application. Application Note

pyrolysis and subsequent analysis

AN-CIC-017 provides more detailed

by ion chromatography in an

information and is available free

integrated, fully automated system.

from www.metrohm.com

In a first step, the sample is digested under an argon atmosphere

C

and pyrolysed in an oven unit. The opper concentrate is often

techniques involve a long cumbersome

resulting gaseous compounds are

contaminated with corrosive

fusion with a potentiometric ion-

transferred into an absorption

fluorine, which is why the fluorine

selective electrode finish. This method

solution, which is then injected

concentration must be checked at

is prone to huge analytical errors.

into an ion chromatograph for

regular intervals. Current laboratory

Combustion ion chromatography (CIC)

subsequent determination.

Metrohm SA Contact: Ravi Issari Tel: 011-656-2686 Email: ravii@metrohm.co.za

Special Chemistries

LMS Issue 3 | 2016

31

Meeting future energy demands

T

he Carbon Technology Materials

and holds the South African

composites, nanocomposites,

layer capacitors whereas oxides

Research Group, based at

Research Chairs Initiative’s Carbon

electrospinning and chemical

and other electrode materials give

Technology and Materials Chair.

sensor technologies.

pseudo-capacitance. The group works

the University of Pretoria (UP), is

“Our research activities focus

currently undertaking tests using

Research

on various forms of carbons materials

on the materials science and

including two and three dimensional,

engineering of these technologies

nanostructured electrode

nanomaterials research with

to meet future energy demands. The

materials for the next generation

leadership of Prof Ncholu

specific applications in areas of

main energy technology focus of

of supercapacitor electrodes.

Manyala, who heads the group

energy storage, high performance

the lab is the supercapacitor mostly

the Bio-Logic VMP 300 multichannel Potentiostat/Galvanostat (PGSTAT).

Testing is being done under

The group is involved in carbon

studied with the use of the Bio-Logic VMP-300 multichannel PGSTAT with EIS boards,” said Prof Manyala.

Supercapacitors are

Graphene The most commonly used material in the laboratory is graphene with focus on the chemical vapour

electrochemical energy storage

deposition growth of graphene as

devices with different characteristics

well as its characterisation, chemical

than battery. While the energy

functionalisation and applications in

density of supercapacitors are

areas such as energy storage.

much smaller than that of battery, the power density can be orders of magnitude higher because they can be charged and discharged very fast.

Carbon materials Carbon materials are commonly

Bruno Steiner in his laboratory

used as electrodes in electric double

Bruno Steiner Lab Consultancy Tel: 011 804 3503 Fax: 086 719 2778 email: bruno@bslc.co.za

Exceptional catalytic activity Easy, fast and safe titration

R

esearchers from China

displaced the chloride ions,

have developed a new

causing the palladium atoms to

technique to disperse palladium

form stable palladium-oxygen

atoms in catalysts. This new

bonds to the nanosheets. The

technique maximises the

catalyst did not need calcination.

availability of the individual noble metal atoms in a catalyst.

In addition, it also allows the

The researchers tested the catalyst by hydrogenating styrene and found that the activity per

atoms to catalyse completely

palladium atom was nine times

different pathways from traditional

higher than in some commercial

noble metal catalysts, drastically

palladium catalysts and remained

increasing the catalyst’s activity.

undiminished after 20 cycles.

Precious metals e.g. platinum

‘Each palladium catalysed

L-R : Justine Anandam, Diane Brijlal

and palladium, catalyse numerous

200 000 bond hydrogenations and

reactions in industry including

we didn’t see any decay,’ said Zheng.

the oxidation of unburned

More remarkably, the catalyst was

P

hydrocarbons in car’s catalytic

over 55 times more active than

manufacturer and supplier

converters. The efficacy of the

other palladium-based catalysts for

of specialty chemicals to the

catalyst per atom is maximiSed by

hydrogenating the carbon oxygen

water treatment industries.

dispersing the noble metal atoms.

double bond in benzaldehyde

rotea Chemicals in Mobeni,

from Metrohm - a preferred

KwaZulu-Natal, is a major

supplier of analytical solutions to

The main product range consists

the water treatment industry.

This package was supplied with all the components necessary to conduct the chloride test,

because it activated hydrogen

of synthetic organic and inorganic

viz. a stirrer, an exchange unit

University (China) et al developed

through a different pathway

coagulants and flocculants for the

for AgNO3 and Ag Titrode

catalysts of atomically-dispersed

that is normally only accessible

potable water, industrial water, pulp

(electrode). The connection of a

palladium on titanium dioxide

with homogeneous catalysts.

and paper and mining industries.

custom thermal printer enables

Nanfeng Zheng of Xiamen

nanosheets produced by reacting TiCl4 with ethylene glycol.

Nanosheets were dispersed in water and H2PdCl4 was added

The researchers are now investigating whether the technique can improve the efficiency of other industrially important catalysts.

traceability of raw data.

Analytical tests An array of analytical tests are performed in the laboratory, one of them being the determination

before the mixture was irradiated with UV light. The catalysts

Reference

were then collected, washed and

Wogan T. Spreading palladium thinly

dried. The UV photons generated

delivers exceptional catalytic activity.

safe titrations, Protea Chemicals

ethylene glycolate radicals, which

Chemistry World, 2016.

invested in an 848 Titrino Plus

of the chloride concentration.

To facilitate fast, easy and

Metrohm SA Contact: Ravi Issari Tel: 011-656-2686 Email: ravii@metrohm.co.za

32

LMS Issue 3 | 2016

Centrifugation

Hettich centrifuge and accessories for washing erythrocytes

H

ettich offers the ideal lab

may be carried out either manually

tubes are held in position

equipment for manual and

or using a cell wash centrifuge.

and are prevented

automated cell washing procedures.

The practical solution from

from falling out when the

Washing of erythrocytes as a process

Hettich for manual washing of

step in blood serology studies (such

erythrocytes: EBA 280 small

as cross matching and antibody

centrifuge with 1133 decanting

throughput:

identification and differentiation)

rotor. Small Hettich centrifuges

12x5ml tubes

is part of the daily routine of

with an angle rotor for 5ml glass

per run.

an immunohaematology lab.

tubes have been successfully

The removal of plasma, which would otherwise interfere with test

used for many years.

Our new centrifuge - the EBA

contents are decanted.

High sample

Powerful with variable

procedures, through h repeated

280 - now offers even greater

speed

washing in physiological saline

comfort through the rapid rotor

entry:

change system. A cell washing

5500 - 5000

centrifuge can be converted

rpm (max.

into a universal lab centrifuge

6000 rpm

in a matter of seconds.

with the EBA

Labotec

whole blood).

High operator

280 S model).

Tel: 011-315-5434

The advantages at a glance:

Fax: 011-315-5882 Email: sales@labotec.co.za

Rotor with decanting aid: The

Maximum flexibility: Six rotors are

comfort: Simple rotor changeover

available for different applications

without tools thanks to the

(such as the derivation of serum from

rapid rotor change.

Growth in global clarifier and centrifugation markets predicted

A

becoming increasingly popular

They lengthen the life of downstream

sewage sludge, coal slurries,

in municipal drinking water

separation devices. One big use is

the global clarifier and centrifugation

slaughterhouse effluent and many

plants to remove relative small

to separate gypsum particles from

markets are set to grow by R50m

slurries. Disk centrifuges are used

particles. Another major use is

the liquid discharge in power plant

and R80m over the next year.

to clarify wine and juices and to

in the purification of water used

flue gas desulfurization systems.

purify pharmaceutical products.

in de-inking of recycled paper.

ccording to the latest forecast by the McIlvaine Company (US)

The clarifier segment includes

Decanter centrifuges dewater

Hydrocyclones are widely used in

Asia represents the biggest market.

Centrifuges are the frequent choice

thickeners. These devices are

There are several other types of

used to remove the larger solids

centrifuges using spinning baskets

the minerals industry for separating

for many of the new sewage treatment

in wastewater plants. They are

and other means for separating

particles from slurries. They are

plants being built in the region.

used for oil/water separation

particles from liquids. Basket

also used in conjunction with more

Hydrocyclones are incorporated in

in the refining industry. They

centrifuges separate valuable

efficient separation devices as a

the many new power plant scrubber

typically are used in conjunction

products in the chemical industry.

pre-separation step. They are rugged

systems underway in China.

and can handle large solids loads.

Source: McIlvaine Company.

with other separation devices.

Dissolved air flotation is

Fastest PCR microcentrifuge in its class

T

from the edge of the tube cap,

preparation, then the rotors can

runs are required, for instance

it is sufficient to press the pulse

be provided with a lid that is

to centrifuge liquid drops down

key. The centrifuge then runs

resistant to phenol. In addition,

of 21 382 has been proven in many

for as long as the key is pressed,

due to the bioseal, the unit can

polymerase chain reaction (PCR)

at the preselected speed.

be autoclaved. The MIKRO 200 is

he efficacy of the Hettich MIKRO 200 centrifuge with its

maximum relative centrifugal force

labs. The MIKRO 200 is appreciated

If only short centrifugation

An added plus is the large range

because of its compact design

of rotors available, from which a

and high performance.

suitable rotor can be selected to

It is one of the fastest

also available with cooling for the preparation of RNA samples.

meet individual requirements.

microcentrifuges in its class. In

If there is a high throughput of

addition, its ergonomic design

samples, a 30-place rotor is available

makes it easy to use on a daily

in addition to the 24-place rotor.

basis. The selection of parameters

A separate rotor is available to

and the input of values, using a

centrifuge eight tube PCR strips.

rotary button is quick and easy.

If phenol is used for sample

Labotec Tel: 011-315-5434 Fax: 011-315-5882 Email: sales@labotec.co.za

34

LMS Issue 3 | 2016

Materials Science/Analysis

Micro laser particle sizer with extra-wide measuring range

G

erman company Fritsch’s

flexibility, an overall measuring

pump for the optimal transport

All this, combined with effective

Analysette 22 MicroTec

range from 0.08µm-2000µm with

of even heavy particles with a

and variable ultrasonic-system,

plus laser sizer has an extra wide

an outstanding resolution of up

high concentration through the

linked with especially flexible

measuring range of 0.08µm-2000µm

to 108 measuring channels.

measurement system. With the help

software, which also manages even

Remarkably powerful: The wet

of an intelligent liquid-level-sensor,

complex measurement sequences.

dispersion unit is equipped with a

three individual amounts of liquids

very powerful

can be set by default and also the

include fast analysis times, good

centrifugal

use of common organic solvents as

reproducibility, ease of operation

suspension liquids is possible.

and calibration, large measurable

and revolutionary dual-lasertechnology for wet measurements.

Practical module-system with fast-switch-system: The measuring cells of the MicroTec plus are located

Additional advantages

particle size ranges.

in convenient cartridges. When you are not momentarily using the cartridge, it’s simply stored in the corresponding dispersion unit.

Variable measuring range: With

Labotec

the MicroTec plus you may choose

Tel: 011-315-5434

between two individual measuring

Fax: 011-315-5882

ranges or combine both to a third.

Email: sales@labotec.co.za

Advantages include maximum

Autolab provides insight into corrosion phenomena orrosion is a process

Credit: Autolab

C

involving deterioration or

High performance with the DSC 3+

M

ettler Toledo’s new DSC

even in the most demanding

3+ differential scanning

application environments.

calorimeter provides unmatched

For example the indium height to

differential scanning calorimetry

width ratio is improved to 19.5mW/°C

losses. As corrosion processes

(DSC) performance. This is due

and higher than 155mW/°C when

are electrochemical in

to its innovative DSC sensors

applying data optimisation. Taking

nature and involve two

with 120 thermocouples which

all this into account, it is evident

or more reactions,

guarantee unbeatable sensitivity

that the DSC sensors combine a

electrochemical

and outstanding resolution.

number of characteristics that are

degradation of materials that results in huge economical

techniques using

A quantum leap in sensor

sophisticated

technology enabled Mettler Toledo to

instruments

offer the highest sensitivity sensors

are required to

available in DSC instrumentation. The

study them.

The Autolab

unattainable with conventional sensors used in other instruments.

The investigation of challenging samples, from very small to large

signal-to-noise ratio, an important

volumes, requires variation of heating

instrument parameter,

rates, crucible types and method

is determined by

gas to properly analyse important

along with the NOVA software

Faraday cage.

the number of

material parameters or transitions.

offer the corrosion practitioners

Rotating disc electrode (RDE).

thermocouples

instruments

a wide array of tools for studying these processes.

The unique built-in FlexCal®

and their specific

Software methods Linear sweep voltammetry:

technology

arrangement.

automatically

All this allows the

applies the

Modules

Identification of

user to measure

correct

FRA32M: Corrosion

corrosion processes.

extremely weak

calibration

thermal effects

measurement

testing and research.

ECN: Characterisation of coatings.

MUX.Multi 4: Sequential

Chrono methods: Identification of pit initiation.

Electrochemical impedance

with sample

measurements on up to 64 cells.

spectroscopy: Detection of

small as a few

pX1000: Critical pitting

coating failure and identification

micrograms

temperature measurements.

of corrosion mechanisms.

and this

EQCM: Determination

parameters

masses as

which results in faster measurements.

even at low heating rates.

The DSC 3+ combines premium

of mass change.

furnace technology combined

Accessories Reference and counter electrodes.

Corrosion cell.

Flat cell.

Normal cells.

Metrohm SA Contact: Ravi Issari Tel: 011-656-2686 Email: ravii@metrohm.co.za

with the latest developments in sensor technology. This newly developed furnace/sensor combination is the perfect solution for long-term stability and durability

Microsep Contact: Rudy Maliepaard Tel: 011-553-2300 Email: rudy.maliepaard@microsep.co.za

36

LMS Issue 3 | 2016

Food & Beverage

Real-time PCR test kits ensure food safety allergens by real-time polymerase chain reaction (PCR) as well

The high quality of Foodproof

assist in efficiently working with all

processing of food samples.

validated and confirmed by

types of sample matrices, including

independent institutes such

difficult complex samples.

screening and quantification kits allow

as Association of Official

developer of rapid micro-

fast, simple and reliable detection

Analytical Chemists, NordVal

methods for the food industry

by real-time PCR in a variety of

and MicroVal certification.

worldwide, offers a range of rapid

samples, such as fruit juices, beer,

detection systems for foodborne

chocolate, baby food, spices or meat.

Analyses are based on real-time

For use with the PCR rapid detection systems, Biotecon Diagnostics has developed fast and

genetically modified organisms

PCR, a simple and proven solution

easy-to-use DNA extraction kits.

(GMOs), animal species and

for the reliable detection of genetic

The Foodproof ShortPrep, StarPrep,

National Separations Contact: Frances Renwick Tel: 011-553-2300 Email: frances.renwick@microsep.co.za

Simple, robust and reliable LC method

Best before...

P

atulin is a toxic substance known as a mycotoxin produced by molds

the analysis of patulin in apple juice.

As figure 1 shows, the analyte is

such as penicillium and aspergillus,

well separated from other matrix

frequently found on fruits and

components in under five minutes

vegetables. Its occurrence has been

by UHPLC using UV detection.

a significant issue in the processing

The average patulin level was

of apple juices. Due to its thermal

calculated to be 71ppb for this

stability, patulin decomposition is

sample, which was consistent with

inhibited during pasteurisation.

the provider’s expectations.

The European Union (EU),

F

preparation kits conveniently

products has repeatedly been

iotecon Diagnostics, a leading

pathogens, spoilage organisms,

sample preparation and magnetic

as instruments for automated The Foodproof® detection,

B

information (nucleic acids).

The studies completed

in Commission Regulation (EC)

demonstrated excellent retention

oodstuffs that contain fat such as

exposed to higher temperatures and

1425/2003, has recommended

time repeatability, as well as very

butter, nuts, cookies, and potato

air in the Rancimat. This method

that the maximum patulin intake

good linearity, over the tested

chips, turn rancid over time. This is

is able to predict the way in which

limit be set to 50 ppb for apple

concentration range. Considering the

caused by chemical changes in the

the fatty acids will break down

juice and apple products.

LOQ level for patulin and the recovery

fat - in particular, its oxidation.

in the future, and how quickly.

Because of this, determining the

The Rancimat method provides

For solid apple products, such

results for actual samples, this

as apple puree, a 25 ppb limit was

application can be applied effective for

oxidation stability of fats and oils in

reliable information about the

recommended. However, for any apple

monitoring patulin in apple juice and

foods has proven to be a useful tool

oxidation stability of fats and oils as

products intended for infants or young

covers the EU’s maximum allowable

in the quality control of foodstuffs.

well as fatty foods. It can also be used

children, the limit was set to 10 ppb.1

limit of 10ppb for small children.

And the Rancimat method is designed

to analyse cosmetics. The established

to perform this exact task.

method is outlined in various national

The Rancimat method turns this dependency on conditions into an advantage, exposing the sample to a higher temperature while

and international standards such as:

1. Commission of the European

Palutin testing Conventional methods for

Directive 1425/2003. Amending

Americal Oil Chemists’

patulin testing require multi-step

Regulation (EC) No 466/2001

Society Cd 12b-92*.

sample preparation and some use

as regarding patulin. s.l..

large quantities of solvents and

Official Journal of the European

Sampling and analysis of

passing a continuous stream of air

commercial fats and oils:

chemicals.2 Using the PerkinElmer

through it. A process that might

Oil Stability Index

Altus ultra-high performance liquid

normally take weeks, months, or

Community; Commission

Internation Standardization

Community Legislation, 2003.

2. Official Method of Analysis.

chromatography (UHPLC) system,

AOAC International. Method

even years is completed within a

Organization 6886: Animal

we were able to develop a simple,

995.10, Gaithersburg, MD.

matter of hours in the Rancimat.

and vegetable fats and oils -

robust, and reliable LC method for

AOAC, June 2000.

The time that elapses until the

determination of oxidative

point when volatile acids are detected

stability (accelerated oxidation

in the measuring vessel is referred to

test 2.4.28.2-93).

as the induction time or oil stability index. This is a measure of oxidation stability: The longer the induction time, the more stable the sample.

The Rancimat method acts as a crystal ball for fat behaviour, thanks to the accelerated ageing that takes place in the sample when it is

Metrohm SA Contact: Ravi Issari Tel: 011-656-2686 Email: ravii@metrohm.co.za

Figure 1: Chromatogram of a sample of apple juice from a manufacturing facility (black) and 0.3μg/mL patulin standard in diluent (red).

Food & Beverage

LMS Issue 3 | 2016

37

Optimise industrial recipes on a smaller scale with new mini freeze dryer

T

elstar has launched a new

on a smaller unit and optimise

research and development

scale up time, reducing time to

freeze-dryer, the new LyoBeta Mini.

The device is a small-scale unit that

market for finished forms.

Applications for the LyoBeta

Saleable units

where you can expect the highest

LyoBeta Mini will be produced at

freeze-drying performance

Telstar’s factory in Terrassa. Saleable

with minimum footprint are:

units are already available to the

Standard configuration includes a

occupies less space in the research

Mini can be found in academic,

lab, but has all the capabilities

industrial pharmaceutical, food

of an industrial freeze-dryer.

or biotechnology markets, both

laboratory and research and

for research and development, as

development freeze-dryers

well as for small productions.

offers high specifications and

5.5 l condenser capacity.

advanced performance for

<-85°C condenser temperature.

optimum freeze-drying results

Vacuum monitoring and control.

throughout all stages of freeze-

Temperature sensors for

These capabilities allow optimisation of industrial recipes

market from local agent, Labotec.

The LyoBeta range of

drying process development.

touch screen for unit control with programmable logic controller.

Shelf temperature control from -55°C to +70°C.

product and shelf monitoring.

Aeration valve.

Key parameters LyoBeta Mini facilitates understanding of the key parameters

Flexible shelf area.

Automatic defrosting.

User-friendly and

of a freeze-drying process and

intuitive interface for easy

provides a very valuable tool

operation.

for process scale-up at stages approaching production. LyoBeta Mini offers similar features to larger units while occupying less space in research laboratories.

Features Some features from the LyoBeta

Labotec Tel: 011-315-5434 Fax: 011-315-5882 Email: sales@labotec.co.za

Get a head start on tighter load cells process control

L

oad cells aren’t all alike, and

It’s industry norm for

Vishay Revere offers most of its

Many of the Vishay Revere

manufacturers to use potting or

load cells in solid stainless steel - a

cells are still performing after 30

differences can put users closer to

epoxy for the element seal. Epoxy

far cry from simple plating. These

years in the field - and are still

total control. All weight-measurement

may test hermetic but it can, over

are not just the products they sell

meeting spec! That is rare unless

functions start with a load cell,

the long run, allow moisture to

for harsh environments. Vishay

the bond is very close to ideal to

so it makes sense that a little

enter. Since long-run is the name

Revere figures that replacing a load

begin with, and stays that way.

difference can have a large effect.

of the game, Vishay Revere uses a

cell is something users shouldn’t

precision, stainless-to-stainless

have to think about more often than,

very special hand operation. Only

weld for the element seal.

say, every thirty or forty years.

Vishay Revere’s best people are

taking a hard look at the

Vishay Revere seals The hermetic seals that

The cable-exit seal requires

A last word about bonding: It’s a

bonders, and their Quality Assurance

Strain-gauge bonding: Dimensionless isolation

isolate the strain gauge from the

a different approach, because it

harsh outer world make a good

involves dissimilar materials. Here

starting point. Vishay Revere

Vishay Revere provides an extra

seals have been engineered

large, extra supportive compression

to address the bonding of the strain

and perfected to withstand

seal to make sure users load cells

gauge into the cell. This is because

Revere’s load cells, or information

severe environmental abuse.

will be as secure ten years hence,

the ideal is so easy to define:

about seminars, demos or to

as it is on the day of installation.

The strain gauge should

Users may not always have to work in a caustic atmosphere, but it is reasuring to know that Vishay Revere load cells can even if users just want to hose things down once in a while.

Corrosion resistance that’s more than just skin deep The material that goes into

It’s rare for load cell manufacturers

bonding techniques, ensuring that they meet the highest standards.

For more information on Vishay

locate the nearest dealer, please

become an inseparable part

contact Instrotech on 010 595 1831,

of the metal surface, without

sales@instrotech.co.za or visit

losing its electrical isolation.

www.instrotech.co.za

Vishay Revere has spent a lot

the load cell is an important

of time perfecting materials and

consideration, too. Again users may

bonding techniques, and the

at two critical areas: Where the

not be expecting their installation

result is evident in the accuracy

strain gauge and element are

to take occasional acid baths, but

and repeatability. The result is

isolated, and where the cable

its comforting to know that Vishay

evident in other ways, too: The

is brought out of the load cell -

Revere’s load cells are even able

better the bond, the longer users

both require special attention.

to hold up to that eventuality.

go on without losing calibration.

Sealing actually takes place

programme puts special emphasis on

The Comtest Group Tel: 010-595-1821 Fax: 011-608-2705 Email: sales@comtest.co.za

38

LMS Issue 3 | 2016

Food & Beverage

Fully integrated sugar analyser

F

Automated Eluent Generation™

ood and beverage manufacturers

Federal Regulation Title 21

of years of collaboration with

perform carbohydrate analysis

for Food and Drugs).

customers in food and beverage

technology - better method

Food and beverage manufacturers

testing laboratories to understand

reproducibility by eliminating

for a variety of reasons, including product development, raw ingredient

and standard-setting agencies

their challenges when it comes

testing, process monitoring, quality

require methods that provide

to routine sugar analysis.

assurance and labelling control.

sensitive, selective, and direct

Testing is performed to:

determination of carbohydrates.

HPIC sugar analyser provides: Direct detection

Recent advances have enabled

using electrochemical

issues such as obesity, diabetes,

companies to analyse more

detection without sample

or cardiovascular diseases.

samples in less time.

derivatisation - saving time.

Understand health-related

Perform constituent testing for new product development.

Comply with food quality standards.

Meet the legal requirements for labelling (Food Information Regulation in Europe and the US Part 1010 of the

Minimising errors associated with sample preparation is also of key importance. Food and

Expense, and generation of hazardous chemical waste.

One system to determine

beverage samples often require

monosaccharides and

large dilutions that can impact the

disaccharides.

downstream accuracy of results.

The Dionex™ Integrion™ HPIC™ sugar analyser is the culmination

A completely metal-free, all-

manual preparation.

A system requiring no expensive or environmentally unfriendly organic solvents.

Optimised detector and injection volumes, precluding the need for large or serial sample dilution.

Fast run times without compromising data quality using high-pressure ion chromatography (IC).

The Dionex™ Integrion™ IC system is made to support you.

PEEK™ flow path for better

It was designed to fit with the

sensitivity and system robustness.

flow of your laboratory so that it quickly falls in sync with your

The following figures illustrate the performance of the Dionex Integrion HPIC Sugar Analyser

processes. Thoughtfully designed instruments accelerate your stream of results while blending seamlessly into your laboratory.

Anatech Instruments Contact: Chris Fourie Tel: 011-462-6776/083-268-3147 Email: chris@anatech.co.za

Fast, nondestructive, fluorescent staining-based system

E

Z-Fluo™ is a nondestructive method that enables you to

continue to grow the microorganisms after they have been stained in order to identify them using any standard ID technology.

This is a tremendous advantage compared to other rapid test systems which cannot ID microorganisms using the same sample that was tested to detect the contamination.

Flexible approach based on

Today, more and more QC

EZ-Fluo is a rapid fluorescent-

standard membrane filtration and

laboratories are switching to

based technology designed for

reduced incubation on media plate.

rapid methods for the detection of

fast quantitative detection of

microbial contamination. This is

microorganisms over a broad range

because traditional methods require

of filterable samples. This new

several days to provide results and

system uses standard membrane

therefore are of limited value when

technology to detect viable and

it comes to releasing product to

culturable micro-organisms

the market faster, or to conducting

down to 1 CFU per sample.

Nondestructive method, compatible with any ID technique.

Compact hardware, fits easily on any laboratory bench.

Cost effective solution for routine use as well as investigation.

quick investigation when necessary.

EZ-Fluo can detect and quantify

EZ-Fluo test results are easily comparable to compendial method

microbial contamination in

test results, which facilitates the

providing results in 1/3 of the

any filterable sample. Potential

validation and the integration of

time required using traditional

applications are numerous

this rapid system in any laboratory

techniques.

and include testing of the

without causing disruption.

following sample types:

Detected microbial contamination

Raw materials.

can then be identified using your

In-process samples.

current ID methodology.

Rapid technology capable of

Final product.

Environmental samples.

The EZ-Fluo Rapid Detection System enables you to reduce your time to result, address contamination events sooner and therefore gain better control of your manufacturing process.

Merck Pty Ltd Tel: 08600 63725 Fax: 0860 522329 Email: labsupply@merckgroup.com

Product Review

LMS Issue 3 | 2016

39

Element Finder plug-in software

I

nterferences are common in

enhancement of signals, and thus,

for users who are new to ICP-OES

automatically optimises the

inductively coupled plasma-optical

false negative or positive results

and have little or no knowledge

plasma based on the elements

which ultimately degrade the

of method development.

present and the sample matrix

emission spectrometry (ICP-OES).

These are commonly encountered in environmental, metallurgy and

and in this way the Element Finder

accuracy and precision of the method.

The Element Finder plug-in

The Thermo Scientific™ Element

uses full frames of the sample (a

plug-in helps to save time as the

geological applications where

Finder Plug-in software for Qtegra™

full frame is an image of the enter

wavelength selection process is

there is a mixture of very high and

ISDS, has been developed to assist

sample spectrum, within the

fully automated removing the need

low concentrations of elements

in automation of method developing

wavelength range of the instrument).

for specialist knowledge.

resulting in a spectrally rich matrix.

for the Thermo Scientific iCAP™

The software intelligently selects

7000 Plus Series ICP-OES, enabling

the wavelengths that are suited

characterised by an overlap (either

simplified, clear, wavelength

(free from potential spectral

partial or direct) of the analyte of

selection and plasma optimization.

interferences) for analysis of the

interest by an interfering element.

This reduces the complexity

elements, and takes into account

Spectral interferences are

These interferences are normally

and time investment needed

the analytes present in the

apparent during method development

for method development while

sample and the sample matrix.

and can lead to suppression or

increasing productivity. It is ideal

The Element Finder plug-in

Anatech Instruments Contact: Maxime Chalmers van den Berg Tel: 011-462-6776/ 076-687-5519 Email: maxime@anatech.co.za

Absorbents for spilled liquids

M

20kg PE drum 1.01568.9021.

ishaps and accidents happen. With Chemizorb®,

collected, the contaminated

2.5kg bucket, plastic

surface is cleaned thoroughly

1.02491.2500.

aggressive or other unpleasant

Hg reagents and accessories for absorbent for mercury

liquids quickly and safely.

One set PE case 1.12576.0001.

however, you can remove spilled

with plenty of water.

Advantages

reagent 1, 100ml of reagent 2,

HF absorbent and neutralizer for spilled hydrofluoric acid, with indicator

With Chemizorb® you can remove

one small tub, one large disposal

1kg PE bottle

spilled liquids easily, quickly, safely

can and protective gloves.

One set consisting of 500g of

Disposal Chemizorb and the absorbed chemicals can be disposed in accordance with the

1.01591.1000.

company regulations and

and environment-friendly.

Hg reagents refill pack

Ordering information

One set consisting of: 500g reagent

Powder absorbent for spilled liquids

1 and 100 ml reagent 2 1.01569.0001.

Handling

national guidelines for the hazardous products

All Chemizorb®

in question.

products are handled in much the same way:

500g PE bottle 1.02051.0500.

OH- absorbent and neutralizer for spilled alkalis, with indicator

Granules absorbent for spilled liquids

1kg PE bottle 1.01596.1000.

1kg PE bottle 1.01568.1000.

Wait until the neutralisation and

5kg Bucket, plastic 1.01568.5000.

H+ absorbent and neutralizer for spilled acids, with indicator

20kg Paper sack 1.01568.9020.

500g PE bottle 1.02491.0500.

After the absorbent has been

Spilled liquids are covered with a sufficient amount of absorbent and, wherever necessary, mixed using a spatula, spoon, or small shovel. absorption processes are complete.

Merck Pty Ltd Field Marketing Manager: Lennet Maduray Tel: +27-11-372 5148 Email: lennet.maduray@merckgroup.com

Fast and secure mass calibration

T

oday mass calibration is time consuming and involves several

later calibration of balances.

Mettler Toledo’s new MC Link 2

Organization 17025 and more.

With the new MC Link 2 mass

on time during the process. The final result is saved to the database for

manual-handling steps. In most cases,

mass calibration software for use

calibration software, all data is

later extraction to the customisable

the data is manually transferred

with manual and automated mass

seamlessly communicated within

certificate printing within the system.

several times between different

comparators, offers a solution that

the system and human influence

systems creating high risk of faulty

covers the full mass calibration

is reduced to its minimum. All

calibration software system, cutting

data generation. The complex physical

process, from the incoming

calculations are performed on line

down labour cost whilst improving

effects, correction calculations and

of the weights and its related

and results can be viewed in the visual

data security and efficiency from the

uncertainty analysis require great

administration to the printing

user interface showing in real-time

incoming stock to the certification

care and sophisticated computation.

of the calibration certificates.

clear diagrams the status of the

and send out of a weight set.

In many laboratories, certain

MC Link 2 professional software

MC Link 2 is a highly efficient mass

calibration process. These result in

physical effects are neglected

meets the requirements of regulations

high data security and efficiency

and uncertainty calculations are

such as International Organization

whilst improving the overall weighing

more an estimation than a real

of Legal Metrology R111, National

performance by reducing handling

analysis. Overall there is a high

Institute of Standards and

steps of the calibration officer.

potential of faulty data generation,

Technology, 6969, NAVLab, JJG 99,

which leads to faulty calibration

Deutsche Akkreditierungsstelle,

and writing is eliminated and the

reference weights being used for

International Standardization

complex calculations are performed

The time-consuming data reading

Microsep Contact: Sandra Reid Tel: 011-553-2300 Email: Sandra.reid@microsep.co.za

40

LMS Issue 3 | 2016

Product Review

New compact inverted routine microscope that does more than ever before

N

ikon’s all-new inverted routine microscope features

Utilising Nikon’s industryleading CFI60 optical system and

Key features Compact,

a compact footprint, newly

LED illumination for diascopic

streamlined

developed contrast observation

and epi-fluorescence imaging, the

body for efficient

method and improved operability.

new Eclipse TS2 microscope offers brilliantly clear images, enabling more efficient cell

IMP Scientific & Precision Equipment Tel: 011-916-5000 / 031-764-2821 / 021-852-6133 / 041-364-0159 / 018-293-333 3 Email: info@imp.co.za

observation.

Better observation with LED illuminator.

culture observation and

Streamlined operation.

documentation.

New contrasting

Furthermore, a newly developed contrast observation method and streamlined operation enhance its use for routine laboratory work.

technique, ‘Emboss contrast’ for economical and easy observation.

New contrast shield for high

S/N epi-florescence observation in bright rooms.

Bacteria in pure water: Controlling a growing problem

D

r Paul Whitehead, Research and Development Laboratory

In the article, challenge tests asses the ability of alternative

ultraviolet irradiation are shown.

Dynamic water systems,

discussed. The article shows how ‘with correct design and

Manager at ELGA LabWater is well

filters to remove contaminants

maintenance regimes and

operation and careful application

known in research and laboratory

and the results of tests using

sanitisation protocols are

of protocols, low microbial specifications can be assured’.

circles for his authoritative articles

Copies of the above article

relating to pure and ultra pure water. One of his contributions is

are available free of charge to

entitled ‘Bacteria in Pure Water -

interested parties from Labotec,

Controlling a growing problem’.

the exclusive local agents for ELGA. and you can e-mail your request

The article discusses how and why bacterial growth can

to natasham@labotec.co.za or

still occur even if organic and

phone 011-315-5434.

inorganic impurities are removed down to the limits of detection.

Dr Whitehead addresses specific operational requirements

Labotec

that need to be considered to

Tel: 011-315-5434

ensure that laboratory water

Fax: 011-315-5882

purifiers are successfully

Email: sales@labotec.co.za

controlling bacterial levels.

Multichannel pipettes offer exceptional performance

M

ettler Toledo’s range of

The manual Rainin Pipet-

mechanical designs that eliminate

Capitalising on recent advances

Lite XLS+ and electronic Rainin

hand strain ensuring the highest

in moulding technology, Rainin has

represent an intelligent advance in

E4 XLS+ offer users durable,

channel-to-channel consistency.

achieved an optimal balance between

both performance and ergonomics.

lightweight liquid ends and new

Rainin multichannel pipettes

This combination of improved

lightness and performance. In

ergonomic comfort and exceptional

addition to new internal designs and

performance helps operators

materials, the new smaller surface-

work more comfortably and

area lip seals require less force and

reduce the risk of error and

reduce drag. Taken together, these

potentially expensive rework.

and other new design elements

Reduced weight and lower

reduce weight and increase reliability

pipetting/tip-ejection forces,

for a more pleasurable and repeatable

combined with improved balance

pipetting experience.

reduce operator fatigue during intensive 96-well plate work.

Enhanced reliability ensures data integrity. Operators concerned about cross-contamination will appreciate XLS+’s reduced stiction (piston stickiness), which helps to eliminate splash-up.

Microsep Contact: Frances Renwick Tel: 011-553-2300 Email: frances.renwick@microsep.co.za

Product Directory

Refer to Company Directory for contact details

amino acids analysis

• AE Solutions • Anatech Instruments • LabDynamics • Shimadzu South Africa • The Sepsci Group

ANAEROBIC WORKSTATION

• Air Filter Maintenance Services • Labotec

AROMA & TASTE ANALYSIS

• The Sepsci Group

ATOMIC ABSORPTION

• Anacon • Anatech Instruments • Chemetrix • IMP Scientific & Precision Equipment • Merck Pty Ltd • Shimadzu South Africa • The Sepsci Group • Thermo Fisher Scientific • Wirsam Scientific & Precision Equipment (GBC)

Atomic force microscopy

• Advanced Laboratory Solutions • Anton Paar Southern Africa • Carl Zeiss • Chemetrix • IMP Scientific & Precision Equipment • Shimadzu South Africa

AUTOANALYSERS & CONSUMABLES

• AE Solutions • Anatech Instruments • IMP Scientific & Precision Equipment • ITP Analytical • Labchem • The Sepsci Group

AUTOCLAVES

• AE Solutions • Air Filter Maintenance Services • Analytical Solutions • Anatech Instruments • Celsius Scientific • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labotec • Thermo Fisher Scientific • The Scientific Group • The Sepsci Group • Trilab Support cc

AUTOIMMUNE

• Bio-Rad Laboratories • The Scientific Group • The Sepsci Group

AUTOMATION

• AE Solutions • Bruker South Africa • Hanna Instruments • IMP Scientific & Precision Equipment • Metrohm SA • The Scientific Group • The Sepsci Group • Thermo Fisher Scientific

BALANCES/COUNTING SCALES

• AE Solutions • Air Filter Maintenance Services • Analytical Solutions • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labchem • LabDynamics • Labotec • Microsep (Mettler Toledo) • Monitoring & Control Labs • Shimadzu South Africa • The Scientific Group • The Sepsci Group • Trilab Support cc • Wirsam Scientific & Precision Equipment (A&D)

BATTERY TESTING

• Bruno Steiner Lab Consultancy (Bio-Logic)

BIOHAZARDS (see Laminar Flow) • Air Filter Maintenance Services • Alphalab Engineering CC • Clean Room Maintenance • Esco Technologies • IMP Scientific & Precision Equipment • Labotec • The Sepsci Group • Umoya Laboratory Extraction Technologies

bioplex

• Bio-Rad Laboratories

BIOTECHNOLOGY EQUIPMENT & CONSUMABLES

• Analytical & Diagnostic Products • Analytical Solutions • Anatech Instruments • Bruker South Africa • Bruno Steiner Lab Consultancy (Kuhner) • Esco Technologies • Ilex South Africa • IMP Scientific & Precision Equipment • ITP Analytical • Labchem • Labotec • Magna Analytical

• Merck Pty Ltd • Microsep (Waters & Mettler Toledo) • National Separations • The Sepsci Group

BITUMEN TESTING

• Advanced Laboratory Solutions • IMP Scientific & Precision Equipment • Labotec • Micron Scientific • Microsep (Mettler Toledo) • Monitoring & Control Labs

BLOOD GROUPING

• Bio-Rad Laboratories • Davies Diagnostics • Ilex South Africa • The Scientific Group • The Sepsci Group

BLOOD TESTING/BLOOD GAS

• BD (Becton Dickinson & Company) • Ilex South Africa • IMP Scientific & Precision Equipment • The Sepsci Group

BLOod transfusion equipmenT & ACCESSORIES

• Bio-Rad Laboratories • The Scientific Group • The Sepsci Group

BLOOD VIRUS

• Anatech Instruments • Bio-Rad Laboratories • Ilex South Africa • The Scientific Group • The Sepsci Group

BLOT KITS (southern, northern, western)

• Analytical & Diagnostic Products • Merck Pty Ltd • National Separations • The Scientific Group • The Sepsci Group

CALIBRATION SERVICE & EQUIPMENT

• AE Solutions • Advanced Laboratory Solutions • Archimedes Laboratory Solutions • Clean Room Maintenance • IMP Scientific & Precision Equipment • Intertek • LabDynamics • Labotec • Magna Analytical • Merck Pty Ltd • Metrohm SA • Microsep • Monitoring & Control Labs • The Sepsci Group • Trilab Support cc • unitemp

calorimeters

• Advanced Laboratory Solutions • IMP Scientific & Precision Equipment • LECO Africa • The Sepsci Group

Cameras (See Digital Cameras)

• AE Solutions • IMP Scientific & Precision Equipment

CARBON/HYDROGEN/NITROGEN SULPHUR ANALYSERS

• AE Solutions • Anatech Instruments • IMP Scientific & Precision Equipment • Labotec • LECO Africa • Magna Analytical • The Sepsci Group

CEMENT TESTING

• Bruker South Africa • Grabner Instruments • IMP Scientific & Precision Equipment • Magna Analytical • Metrohm SA • Monitoring & Control Labs • PANalytical • Shimadzu South Africa • Spectro

CENTRIFUGES

• AE Solutions • Air Filter Maintenance Services • Analytical & Diagnostic Products • Analytical Solutions • Anatech Instruments • Bio-Rad Laboratories • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labchem • Labotec • Merck Pty Ltd • Monitoring & Control Labs • National Separations • The Scientific Group • The Sepsci Group • Thermo Fisher Scientific • Trilab Support cc

CERAMICWARE

• Air Filter Maintenance Services

• IMP Scientific & Precision Equipment • Labchem

CERTIFIED REFERENCE MATERIALS & standards

• Anatech Instruments • Bymaz Pty Ltd • Davies Diagnostics • De Bruyn Spectroscopic Solutions • IMP Scientific & Precision Equipment • Industrial Analytical • Labchem • LECO Africa • Merck Pty Ltd • Microsep (Waters, ERA & Romil) • PANalytical • The Sepsci Group

CHEMICALs & REAGENTS

• Air Filter Maintenance Services • Anatech Instruments • Hach South Africa • Hanna Instruments • Industrial Analytical • Labchem • Merck Pty Ltd • Metrohm SA • Microsep (Waters, Romil & Recipe) • Monitoring & Control Labs • The Sepsci Group

CHEMILUMINESCENCE consumables

• Merck Pty Ltd • Microsep (Hygiena International) • The Sepsci Group

CHROMATOGRAPHY DATA SYSTEMS & SOFTWARE

• Anatech Instruments • Anton Paar Southern Africa • Bio-Rad Laboratories • Chemetrix • LabDynamics • LabHouse • Metrohm SA • Microsep (Waters) • The Sepsci Group • Trilab Support cc

CHROMATOGRAPHY ION/GAS/LIQUID/THIN LAYER

• Analytical & Diagnostic Products • Analytical Solutions • Anatech Instruments • Anton Paar Southern Africa • Chemetrix • IMP Scientific & Precision Equipment • LabHouse • Labotec • Merck Pty Ltd • Metrohm SA • Microsep (Waters) • National Separations • Shimadzu South Africa • The Sepsci Group • Thermo Fisher Scientific

Circulating Coolers

• Analytical Solutions

CIRCULATORS

• AE Solutions • Air Filter Maintenance Services • Analytical Solutions • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labchem • Labotec • Magna Analytical • Monitoring & Control Labs • The Sepsci Group • Thermo Fisher Scientific

CLEAN ROOM EQUIPMENT

• Air Filter Maintenance Services • Clean Room Maintenance • Esco Technologies • IMP Scientific & Precision Equipment • Labotec • Micron Scientific • Microsep (Lighthouse) • The Sepsci Group

CLEANING AGENTS

• Merck Pty Ltd • The Scientific Group

CLIMATIC CHAMBERS/ environmental

• Advanced Laboratory Solutions • Air Filter Maintenance Services • IMP Scientific & Precision Equipment • Labotec • Magna Analytical • The Sepsci Group • Wirsam Scientific & Precision Equipment (Espec)

CLINICAL ANALYSERS

• The Scientific Group • The Sepsci Group

COAGULATION

• Bio-Rad Laboratories • The Scientific Group • The Sepsci Group

LMS Issue 3 | 2016

41

COAL ANALYSIS EQUIPMENT

• Bruker South Africa • Carl Zeiss • Chemetrix • IMP Scientific & Precision Equipment • Labotec • LECO Africa • Magna Analytical • Microsep (Mettler Toledo) • Monitoring & Control Labs • PANalytical

COD ANALYSERS

• AE Solutions • Anatech Instruments • Hach South Africa • Hanna Instruments • Labotec • Magna Analytical • Merck Pty Ltd • Metrohm SA

COLONY COUNTERS

• AE Solutions • Anatech Instruments • Instrulab - A division of Air & Vacuum Technologies • Labchem • Microsep (Interscience) • National Separations • The Scientific Group • The Sepsci Group

COLOUR MEASUREMENT

• AE Solutions • Hach South Africa • Hanna Instruments • Merck Pty Ltd • Poretech

COLUMNS

• Anatech Instruments • Merck Pty Ltd • Metrohm SA • Microsep (Waters) • Shimadzu South Africa • The Sepsci Group • Thermo Fisher Scientific

COMPRESSORS

• Instrulab - A division of Air & Vacuum Technologies • Labotec

COMPUTER EQUIPMENT, SOFTWARE & REPAIRS

• AE Solutions • LabInfo (TSI)

CONCRETE testing equipment

• Advanced Laboratory Solutions • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Monitoring & Control Labs • Wirsam Scientific & Precision Equipment (Toni Technic)

CONDUCTIVITY

• Anatech Instruments • Hach South Africa • Hanna Instruments • Labchem • Labotec • Merck Pty Ltd • Metrohm SA • Microsep (Mettler Toledo) • Monitoring & Control Labs • The Sepsci Group • Wirsam Scientific & Precision Equipment (Eutech)

corrosion

• Bruno Steiner Lab Consultancy (Bio-Logic) • Metrohm SA

CRUCIBLES

• Anatech Instruments • De Bruyn Spectroscopic Solutions • IMP Scientific & Precision Equipment • Labchem

CRYO EQUIPMENT

• AE Solutions • IMP Scientific & Precision Equipment • Labchem • Labotec • Magna Analytical • The Sepsci Group

CULTURE MEDIA & accessories

• Air Filter Maintenance Services • Analytical & Diagnostic Products • Anatech Instruments • BD (Becton Dickinson & Company) • Davies Diagnostics • Merck Pty Ltd • Microsep • National Separations • Quantum Biotechnologies • Selecta-MEDIA • The Sepsci Group

cuvettes & cells

• Anacon • Anatech Instruments • Chemetrix • Hach South Africa • IMP Scientific & Precision Equipment • Labchem • Labotec • Merck Pty Ltd

42

LMS Issue 3 | 2016

Product Directory

data loggers & systems

• Monitoring & Control Labs • unitemp

deionisers

• Air Filter Maintenance Services • IMP Scientific & Precision Equipment • Labotec • Merck Pty Ltd • Microsep • National Separations • The Sepsci Group

densitometers

• The Sepsci Group

density specific gravity meters & scales

• IMP Scientific & Precision Equipment • Labotec • Microsep (Mettler Toledo) • The Sepsci Group

DERIVATIZING AGENTS

• Anatech Instruments • Merck Pty Ltd

Dessicators

• Air Filter Maintenance Services • IMP Scientific & Precision Equipment • Labchem • Labotec • Merck Pty Ltd

dETECTORS

• Anatech Instruments • Instrulab - A division of Air & Vacuum Technologies • Bruker South Africa • Metrohm SA • PANalytical • Labotec

DEWAR FLASKS

dIABETES MONITORING

• Bio-Rad Laboratories • The Scientific Group • The Sepsci Group

diagnostic standards

• Ilex South Africa • Industrial Analytical • Microsep (Recipe) • The Sepsci Group

dIAmond knives

• Advanced Laboratory Solutions • The Scientific Group

diffractometers

• Bruker South Africa • Magna Analytical • PANalytical • Shimadzu South Africa • Thermo Fisher Scientific • Wirsam Scientific & Precision Equipment (Rigaku)

digestion

• AE Solutions • Analytical Solutions • Anatech Instruments • Labotec • Magna Analytical • Merck Pty Ltd

digital cameras

• Advanced Laboratory Solutions • Carl Zeiss • IMP Scientific & Precision Equipment

Dimensional Measuring

• IMP Scientific & Precision Equipment

discrete analysers

• AE Solutions • Analytical Solutions • Anatech Instruments • ITP Analytical • Labotec

disintergration

• LabDynamics • PA Cuthbert

dispensers/dosing

• Air Filter Maintenance Services • Analytical & Diagnostic Products • Analytical Solutions • Labotec • Merck Pty Ltd • Metrohm SA • Microsep (Interscience, Mettler Toledo & Rainin) • Thermo Fisher Scientific

dissolution

• Anton Paar Southern Africa • Chemetrix • LabDynamics • Merck Pty Ltd • Microsep (Hanson Research) • PA Cuthbert • The Sepsci Group

dissolved oxygen

• AE Sol utions • Anatech Instruments

• Hach South Africa • Hanna Instruments • Labchem • Labotec • Merck Pty Ltd • Microsep (Mettler Toledo) • Monitoring & Control Labs • The Sepsci Group

distillation

• AE Solutions • Chemetrix • Grabner Instruments • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labchem • Labotec • Magna Analytical • Merck Pty Ltd • The Sepsci Group

dna testing

• Anatech Instruments • Davies Diagnostics • Dehteq (Pty) Ltd • Ilex South Africa • Merck Pty Ltd • Shimadzu South Africa • The Scientific Group • The Sepsci Group

drug testing

• AE Solutions • Analytical & Diagnostic Products • Bruker South Africa • Chemetrix • Davies Diagnostics • The Sepsci Group

Refer to Company Directory for contact details

embedding media

• IMP Scientific & Precision Equipment • Labotec • Merck Pty Ltd

Environmental Monitoring/Equipment

• AE Solutions • Analytical & Diagnostic Products • Anatech Instruments • Bruker South Africa • Hach South Africa • Hanna Instruments • IMP Scientific & Precision Equipment • Intertek • Labotec • Magna Analytical • Merck Pty Ltd • Metrohm SA • Micron Scientific • Microsep (Waters, Mettler Toledo, ERA, Horiizon Technology & Lighthouse) • Shimadzu South Africa • Spectro • unitemp

extraction systems

• AE Solutions • Air Filter Maintenance Services • Anatech Instruments • Esco Technologies • IMP Scientific & Precision Equipment • Magna Analytical • Polimatrix SA • Umoya Laboratory Extraction Technologies

fat determination

dryers

• AE Solutions • Analytical Solutions • Anatech Instruments • Bruker South Africa • Labotec • Magna Analytical

electrochemical analysers

• Analytical Solutions • Labotec

• Air Filter Maintenance Services • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labotec • Analytical Solutions • Anatech Instruments • Bruno Steiner Lab Consultancy (Bio-Logic) • Hach South Africa • IMP Scientific & Precision Equipment • Labotec • Metrohm SA • Wirsam Scientific & Precision Equipment (Eutech)

electrodes

• Analytical Solutions • Anatech Instruments • Anton Paar Southern Africa • Hach South Africa • Hanna Instruments • Labotec • Merck Pty Ltd • Metrohm SA • Microsep (Mettler Toledo) • The Sepsci Group • Wirsam Scientific & Precision Equipment (Eutech)

electrolyte Analysers

• Ilex South Africa • The Sepsci Group

Electron microscopes, equipment & consumables

• Advanced Laboratory Solutions • Bruker South Africa • Carl Zeiss • Chemetrix • IMP Scientific & Precision Equipment • The Scientific Group • Wirsam Scientific & Precision Equipment

electrophoresis

• Analytical & Diagnostic Products • Anatech Instruments • Labotec • Merck Pty Ltd • The Sepsci Group

elemental analysers

• AE Solutions • Anatech Instruments • Bruker South Africa • IMP Scientific & Precision Equipment • LECO Africa • Magna Analytical • PANalytical • Shimadzu South Africa • Spectro • Thermo Fisher Scientific • Wirsam Scientific & Precision Equipment

Elisa equipment & reagents/test kits

• AE Solutions • Analytical & Diagnostic Products • Anatech Instruments • Bio-Rad Laboratories • Davies Diagnostics • Merck Pty Ltd • Microsep (Mabtech) • The Scientific Group • The Sepsci Group

eM Sample preparation

• Advanced Laboratory Solutions • IMP Scientific & Precision Equipment • Magna Analytical

Fermentors - lab

FERMENTORS - PROCESS

• Labotec

FIBRE OPTICS

• IMP Scientific & Precision Equipment • Labotec

Filtering equipment & consumables

• Anatech Instruments • Clean Room Maintenance • Merck Pty Ltd • Microsep • Monitoring & Control Labs • National Separations • The Sepsci Group

fine chemicals

• Industrial Analytical • Labchem • Merck Pty Ltd • Monitoring & Control Labs

flash chromatography

• Anatech Instruments

• The Sepsci Group • Thermo Fisher Scientific

Fraction collectors

• Anatech Instruments • Labotec • Shimadzu South Africa • The Sepsci Group • PA Cuthbert

freeze dryers

• Advanced Laboratory Solutions • Esco Technologies • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labotec • PA Cuthbert

FREEZERS/ULTRA LOW freezers

• AE Solutions • Air Filter Maintenance Services • Analytical Solutions • Esco Technologies • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labotec • Magna Analytical • Monitoring & Control Labs • The Scientific Group • The Sepsci Group • Thermo Fisher Scientific

FRIDGES

• AE Solutions • Air Filter Maintenance Services • Esco Technologies • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labotec • The Scientific Group • The Sepsci Group • Thermo Fisher Scientific

FTIR

• Analytical Solutions • Anatech Instruments • Bruker South Africa • Chemetrix • Grabner Instruments • Metrohm SA • Microsep (Mettler Toledo) • Shimadzu South Africa • The Sepsci Group • Thermo Fisher Scientific

FUEl cell

• Bruno Steiner Lab Consultancy (Bio-Logic) • Metrohm SA

FUME HOODS/CUPBOARDS

• Air Filter Maintenance Services • Alphalab Engineering CC • Clean Room Maintenance • Esco Technologies • IMP Scientific & Precision Equipment • Labotec • Magna Analytical • The Sepsci Group • Thermo Fisher Scientific • Polimatrix SA • Umoya Laboratory Extraction Technologies

flash point testers

• Grabner Instruments • IMP Scientific & Precision Equipment • Monitoring & Control Labs • Wirsam Scientific & Precision Equipment

flow injection

• AE Solutions • Analytical Solutions • Anatech Instruments • ITP Analytical

fluorescence

• AE Solutions • Analytical & Diagnostic Products • Anatech Instruments • Bruker South Africa • Chemetrix • IMP Scientific & Precision Equipment • LabDynamics • Merck Pty Ltd • PANalytical • Shimadzu South Africa • The Scientific Group • The Sepsci Group

Food analysis equipment

• AE Solutions • Analytical & Diagnostic Products • Analytical Solutions • Bruker South Africa • Davies Diagnostics • IMP Scientific & Precision Equipment • Labchem • Labotec • LECO Africa • Magna Analytical • Merck Pty Ltd • Metrohm SA • Microsep (Waters, Mettler Toledo, Vicam, Hygiena International & Interscience) • National Separations • PANalytical • Shimadzu South Africa

FRIABILITY

FURNITURE

• Air Filter Maintenance Services • Alphalab Engineering CC • IMP Scientific & Precision Equipment • Polimatrix SA • Umoya Laboratory Extraction Technologies

FUSION EQUIPMENT/ CONSUMABLES

• IMP Scientific & Precision Equipment • Industrial Analytical • Magna Analytical • PANalytical

GAMMA COUNTERS

• The Sepsci Group

GAS ANALYSERS

• AE Solutions • Anatech Instruments • Anton Paar Southern Africa • Bruker South Africa • Chemetrix • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • LabHouse • Labotec • Magna Analytical • Scientific Supply Services • The Sepsci Group • Wirsam Scientific & Precision Equipment

GAS GENERATORS

• Afrox • Anatech Instruments • Anton Paar Southern Africa • Chemetrix • Davies Diagnostics • LECO Africa

GAS Installations

• LECO Africa

Product Directory

Refer to Company Directory for contact details

GAS TESTING

• Anton Paar Southern Africa • Bruker South Africa • Chemetrix • Microsep (Lighthouse)

GASES - SPECIALTY

• Shimadzu South Africa

GC/GC-MS

• Analytical Solutions • Anatech Instruments • Anton Paar Southern Africa • Bruker South Africa • Chemetrix • IMP Scientific & Precision Equipment • LabHouse • LECO Africa • Merck Pty Ltd • Microsep (Waters) • Scientific Supply Services • Shimadzu South Africa • The Sepsci Group • Thermo Fisher Scientific • Trilab Support cc

Gel permeation chromatography

• Anatech Instruments • Chemetrix • Micron Scientific • Shimadzu South Africa • The Sepsci Group

GEL TESTING

• Anatech Instruments • IMP Scientific & Precision Equipment

GENE TRANSFER

• Anatech Instruments • The Sepsci Group

GENERAL LABORATORY consumables

• Air Filter Maintenance Services • BD (Becton Dickinson & Company) • Davies Diagnostics • Hanna Instruments • IMP Scientific & Precision Equipment • ITP Analytical • Labchem • LabDynamics • Merck Pty Ltd • The Scientific Group • The Sepsci Group • Thermo Fisher Scientific

Genetic Disorders

• Anatech Instruments • The Sepsci Group

GENOMICS

• Anatech Instruments • Labotec • Merck Pty Ltd • The Sepsci Group

GENOTYPING

• Anatech Instruments • BD (Becton Dickinson & Company) • IMP Scientific & Precision Equipment • The Sepsci Group • Labotec

GERMINATORS

GLASSWARE/GLASS BLOWERS

• Labchem • Thermo Fisher Scientific

GLASSWARE WASHERS

• Air Filter Maintenance Services • Instrulab - A division of Air & Vacuum Technologies • The Sepsci Group

Grain grading equipment

• AE Solutions • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies

Green histology solutions

• Merck Pty Ltd

GRINDING VESSELS

• IMP Scientific & Precision Equipment • Magna Analytical

• Anatech Instruments • LabHouse • Shimadzu South Africa

Heating & COOLING BLOCKS

• Instrulab - A division of Air & Vacuum Technologies

Heating mantles

• AE Solutions • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labchem • Labotec • Thermo Fisher Scientific

HISTOLOGY EQUIPMENT & CONSUMABLES

• Anatech Instruments • IMP Scientific & Precision Equipment • Labotec • Merck Pty Ltd • Microsep (Mabtech)

HOLLOW CATHODE LAMPS

• Anacon • Anatech Instruments • Chemetrix • IMP Scientific & Precision Equipment • Labchem • LabDynamics • Thermo Fisher Scientific • Wirsam Scientific & Precision Equipment

HOMOGENISERS

• AE Solutions • Anatech Instruments • IMP Scientific & Precision Equipment • Labotec • Merck Pty Ltd • Monitoring & Control Labs • National Separations • PA Cuthbert • The Sepsci Group

HOT PLATES/STIRRERS

• AE Solutions • Air Filter Maintenance Services • Hanna Instruments • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labchem • Labotec • Magna Analytical • Merck Pty Ltd • Monitoring & Control Labs • The Sepsci Group • Thermo Fisher Scientific • Trilab Support cc

HPLC/GC/MS EQUIPMENT & CONSUMABLES

• Analytical Solutions • Anatech Instruments • Anton Paar Southern Africa • Bruker South Africa • Chemetrix • IMP Scientific & Precision Equipment • LabDynamics • LECO Africa • Merck Pty Ltd • Microsep (Waters) • National Separations • Shimadzu South Africa • The Scientific Group • The Sepsci Group • Thermo Fisher Scientific • Trilab Support cc

HPLC/MS

• Anatech Instruments • Anton Paar Southern Africa • Bruker South Africa • Bruno Steiner Lab Consultancy (Advion) • Chemetrix • Merck Pty Ltd • Metrohm SA • Microsep (Waters) • Shimadzu South Africa • The Sepsci Group • Thermo Fisher Scientific • Trilab Support cc

HUMIDITY MEASUREMENT

• IMP Scientific & Precision Equipment • Labotec • unitemp

HYDROMETERS

HAEMATOLOGY EQUIPMENT

• Air Filter Maintenance Services • IMP Scientific & Precision Equipment • Merck Pty Ltd • Monitoring & Control Labs

HARDNESS

• Air Filter Maintenance Services • IMP Scientific & Precision Equipment • Labotec • Monitoring & Control Labs • Trilab Support cc

• Davies Diagnostics • Merck Pty Ltd • The Scientific Group • The Sepsci Group

• AE Solutions • Advanced Laboratory Solutions • Hanna Instruments • IMP Scientific & Precision Equipment • Labotec • LECO Africa • Metrohm SA • PA Cuthbert • Wirsam Scientific & Precision Equipment

HEADSPACE ANALYSERS

• Analytical Solutions

HYGROMETERS

ICP & consumables

• Anatech Instruments • Chemetrix • De Bruyn Spectroscopic Solutions • IMP Scientific & Precision Equipment • ITP Analytical • Merck Pty Ltd • Shimadzu South Africa • Spectro

• The Sepsci Group • Thermo Fisher Scientific • Wirsam Scientific & Precision Equipment

ICP-MS

• Anatech Instruments • Anton Paar Southern Africa • Chemetrix • De Bruyn Spectroscopic Solutions • IMP Scientific & Precision Equipment • Merck Pty Ltd • Microsep (Waters) • Shimadzu South Africa • Spectro • The Sepsci Group • Thermo Fisher Scientific

IMAGE ANALYSIS/ data processing

• Analytical & Diagnostic Products • Carl Zeiss • IMP Scientific & Precision Equipment • Labotec • LECO Africa • Micron Scientific • Microsep (Interscience) • The Sepsci Group • Wirsam Scientific & Precision Equipment

IMAGING INSTRUMENTS

• Advanced Laboratory Solutions • AE Solutions • Analytical & Diagnostic Products • Bruker South Africa • Chemetrix • IMP Scientific & Precision Equipment • Micron Scientific • Shimadzu South Africa • The Scientific Group • The Sepsci Group • Thermo Fisher Scientific • unitemp

IMMUNO-HAEMATOLOGY

• Bio-Rad Laboratories • Davies Diagnostics • Ilex South Africa • IMP Scientific & Precision Equipment • Labotec • Merck Pty Ltd

IMMUNOLOGY EQUIPMENT

• Bio-Rad Laboratories • IMP Scientific & Precision Equipment • Merck Pty Ltd • Microsep (Mabtech) • The Sepsci Group

impact testing

• Advanced Laboratory Solutions • IMP Scientific & Precision Equipment • Wirsam Scientific & Precision Equipment

INCUBATORS (See Ovens, Furnaces & Incubators)

• AE Solutions • Air Filter Maintenance Services • Analytical & Diagnostic Products • Anatech Instruments • Esco Technologies • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labchem • Labotec • Monitoring & Control Labs • National Separations • The Sepsci Group

Infra-red spectroscopy

• Analytical Solutions • Anatech Instruments • Bruker South Africa • Chemetrix • Grabner Instruments • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • The Sepsci Group • Thermo Fisher Scientific

Integrated chemistry analysers

• AE Solutions • The Sepsci Group

Ion analysis

• Anatech Instruments • Hach South Africa • ITP Analytical • Labotec • Merck Pty Ltd • Metrohm SA • Microsep (Waters & Mettler Toledo) • Shimadzu South Africa

Job costing

• The Sepsci Group

kjeldahl

• AE Solutions • Analytical Solutions • Hach South Africa • Labotec • Magna Analytical • Metrohm SA • Microsep (Mettler Toledo) • The Sepsci Group

LMS Issue 3 | 2016

43

Laboratories independent testing

• Intertek

Laboratory Emergency Body & Eye Showers

• Alphalab Engineering CC • Walker Crosweller

Laboratory & GMP TRAINING

• Bruno Steiner Lab Consultancy • Intertek • LabHouse • The Sepsci Group

Laboratory/sales recruitment

• The Sepsci Group

Laboratory Fittings/Valves

• Walker Crosweller

Laboratory Furniture

• Polimatrix SA

Laminar flow/biohazards

• Air Filter Maintenance Services • Alphalab Engineering CC • Clean Room Maintenance • Esco Technologies • IMP Scientific & Precision Equipment • Labotec • The Sepsci Group • Thermo Fisher Scientific • Umoya Laboratory Extraction Technologies

Laser systems

• IMP Scientific & Precision Equipment • Labotec • Wirsam Scientific & Precision Equipment

Leak detectors

• Anton Paar Southern Africa • Chemetrix • Instrulab - A division of Air & Vacuum Technologies • Labotec

leakwise oil-on-water

• Anatech Instruments

LIMS

• Bika Lab Systems • IMP Scientific & Precision Equipment • LabInfo (TSI) • Microsep (Waters & Mettler Toledo) • Intertek • PANalytical

lUBrICANTS

luminometers & reagents

• Analytical & Diagnostic Products • Dehteq (Pty) Ltd • Ilex South Africa • Microsep (Celsis & Hygiena International) • The Sepsci Group

macro digital systems

• IMP Scientific & Precision Equipment

magnetic stirrers

• AE Solutions • Air Filter Maintenance Services • Hanna Instruments • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labchem • Labotec • Magna Analytical • Merck Pty Ltd • Metrohm SA • Monitoring & Control Labs • The Sepsci Group • Thermo Fisher Scientific

Magnets

• Bio-Rad Laboratories • Labotec • AE Solutions

Magnifiers

Mass spectrometers

• Anatech Instruments • Anton Paar Southern Africa • Bruno Steiner Lab Consultancy (Advion) • Bruker South Africa • Chemetrix • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • LabDynamics • Labotec • LECO Africa • Microsep (Waters) • Shimadzu South Africa • Thermo Fisher Scientific • Wirsam Scientific & Precision Equipment

Material sectioning

• Advanced Laboratory Solutions • IMP Scientific & Precision Equipment • LECO Africa

44

LMS Issue 3 | 2016

Product Directory

materials testing

• Advanced Laboratory Solutions • Bruker South Africa • IMP Scientific & Precision Equipment • Metrohm SA • Microsep (Mettler Toledo) • PANalytical • Wirsam Scientific & Precision Equipment

Melt flow index

• Advanced Laboratory Solutions • Bruker South Africa • IMP Scientific & Precision Equipment

melting point apparatus

• IMP Scientific & Precision Equipment • Labotec • Microsep (Mettler Toledo)

mercury analysers

• AE Solutions • Anatech Instruments • LECO Africa • Magna Analytical • Metrohm SA

Metallurgical sample prep/metallography

• Advanced Laboratory Solutions • IMP Scientific & Precision Equipment • LECO Africa • Magna Analytical

Microanalysis

• Advanced Laboratory Solutions • Bruker South Africa • IMP Scientific & Precision Equipment • Wirsam Scientific & Precision Equipment

Microarray

• Anatech Instruments • The Sepsci Group

microbiology

• Analytical & Diagnostic Products • Anatech Instruments • Bio-Rad Laboratories • Davies Diagnostics • Dehteq (Pty) Ltd • Hach South Africa • IMP Scientific & Precision Equipment • Intertek • Labchem • Labotec • Merck Pty Ltd • Microsep • National Separations • Quantum Biotechnologies • The Sepsci Group • Thermo Fisher Scientific

Microfiltration

• Anatech Instruments • IMP Scientific & Precision Equipment • Merck Pty Ltd • Microsep • National Separations • The Sepsci Group

microplate readers/ harvesters/washers

• Analytical & Diagnostic Products • Bio-Rad Laboratories • Merck Pty Ltd • The Scientific Group • The Sepsci Group

microscopy

• AE Solutions • Advanced Laboratory Solutions • Analytical & Diagnostic Products • Analytical Solutions • Carl Zeiss • IMP Scientific & Precision Equipment • Merck Pty Ltd • Micron Scientific • The Scientific Group • The Sepsci Group • Thermo Fisher Scientific • Trilab Support cc • Wirsam Scientific & Precision Equipment

Microtomes & accessories

• Carl Zeiss • IMP Scientific & Precision Equipment • Labotec • The Scientific Group

microwave

• IMP Scientific & Precision Equipment • Magna Analytical

Microwave Digestion

• Magna Analytical

mixers/blenders

• AE Solutions • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labchem • Labotec • Merck Pty Ltd • Microsep (Interscience) • PA Cuthbert • The Sepsci Group

Moisture analysis

• AE Solutions • Analytical Solutions • Bruker South Africa • Hanna Instruments • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labotec • LECO Africa • Magna Analytical • Metrohm SA • Microsep (Mettler Toledo) • Shimadzu South Africa • The Sepsci Group • Trilab Support cc

Mortuary equipment

• Labotec

mycotoxin analysis

• AE Solutions • Analytical & Diagnostic Products • Anatech Instruments • Anton Paar Southern Africa • Chemetrix • Microsep (Waters & Vicam)

nanotechnology

• Advanced Laboratory Solutions • Analytical Solutions • Bruker South Africa • Bruno Steiner Lab Consultancy (Bio-Logic) • Carl Zeiss • IMP Scientific & Precision Equipment • Labotec • Metrohm SA • Micron Scientific • Microsep (Waters & Lighthouse) • PANalytical • The Scientific Group • Wirsam Scientific & Precision Equipment (Nanosurf & Horiba)

near infra-red analysers

• AE Solutions • Analytical Solutions • Anatech Instruments • Bruker South Africa • Chemetrix • Labotec • Metrohm SA • PANalytical • Thermo Fisher Scientific

nmr

• Advanced Laboratory Solutions • Anatech Instruments • Bruker South Africa • Merck Pty Ltd

oil testing

• Bruker South Africa • Intertek • Magna Analytical • Metrohm SA • Microsep (Waters & Mettler Toledo) • Monitoring & Control Labs • PANalytical • unitemp

Oligonucleotides synthesis

• The Sepsci Group

optical emission

• Chemetrix • IMP Scientific & Precision Equipment • Magna Analytical • PANalytical • Shimadzu South Africa • Thermo Fisher Scientific

optical testing

• IMP Scientific & Precision Equipment • Magna Analytical • Labotec

ORBITAL SHAKERS

Ovens, furnaces & incubators • Air Filter Maintenance Services • Bruno Steiner Lab Consultancy (Kuhner) • Celsius Scientific • Esco Technologies • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labchem • LabDynamics • Labotec • Magna Analytical • Monitoring & Control Labs • The Sepsci Group • Thermo Fisher Scientific • Trilab Support cc

Oxygen Calorimeter

• Digital Data Systems

Paint testing equipment

• Advanced Laboratory Solutions • Instrulab - A division of Air & Vacuum Technologies • Labotec • Metrohm SA • Monitoring & Control Labs

Particle size measurement

• Analytical Solutions • Clean Room Maintenance • IMP Scientific & Precision Equipment

Refer to Company Directory for contact details

• Instrulab - A division of Air & Vacuum Technologies • Labotec • Magna Analytical • Micron Scientific • Microsep (Mettler Toledo & Lighthouse) • Poretech • Shimadzu South Africa • Wirsam Scientific & Precision Equipment

Pathogen testing equipment

• Air Filter Maintenance Services • Analytical & Diagnostic Products • Anatech Instruments • Dehteq (Pty) Ltd • Merck Pty Ltd • The Sepsci Group

Pcr/real-time pcr instruments & Consumables • Air Filter Maintenance Services • Analytical Solutions • Anatech Instruments • BD (Becton Dickinson & Company) • Bio-Rad Laboratories • Davies Diagnostics • Dehteq (Pty) Ltd • Esco Technologies • Ilex South Africa • Labchem • Labotec • Merck Pty Ltd • The Sepsci Group

petroleum testing

• AE Solutions • Anatech Instruments • Bruker South Africa • Chemetrix • Grabner Instruments • Intertek • LabDynamics • Labotec • Magna Analytical • Metrohm SA • Microsep (Waters & Mettler Toledo) • Monitoring & Control Labs • The Sepsci Group • Thermo Fisher Scientific • Wirsam Scientific & Precision Equipment

PFA LABWARE (Moulded Fluoropolymer)

• ITP Analytical • Labchem

ph instrumentation

• Analytical Solutions • Anton Paar Southern Africa • Celsius Scientific • Chemetrix • Hach South Africa • Hanna Instruments • IMP Scientific & Precision Equipment • Labchem • Labotec • Merck Pty Ltd • Metrohm SA • Microsep (Mettler Toledo) • Monitoring & Control Labs • The Sepsci Group • Trilab Support cc • unitemp • Wirsam Scientific & Precision Equipment

Platinum laboratory ware

• Air Filter Maintenance Services • Industrial Analytical • PANalytical

polarimeters

• Instrulab - A division of Air & Vacuum Technologies • Monitoring & Control Labs • Trilab Support cc • Poretech

Porosimeters potentiostats/ galvanostats

• Bruno Steiner Lab Consultancy (Bio-Logic) • Metrohm SA

Power supplies

• The Sepsci Group

pre-analytical tools

• Magna Analytical

profile projectors

• IMP Scientific & Precision Equipment

protein analysis

• AE Solutions • Analytical Solutions • Anatech Instruments • Bruker South Africa • Intertek • Labotec • LECO Africa • Magna Analytical • Merck Pty Ltd • Metrohm SA • Microsep (Waters) • Shimadzu South Africa • The Sepsci Group • Thermo Fisher Scientific

proteomics

• Anatech Instruments • Anton Paar Southern Africa • Bruker South Africa • Chemetrix • Merck Pty Ltd • Micron Scientific • Microsep (Waters) • The Sepsci Group • Thermo Fisher Scientific

pumps

• Anatech Instruments • Hanna Instruments • Instrulab - A division of Air & Vacuum Technologies • Labotec • Merck Pty Ltd • National Separations • The Sepsci Group

Purge & trap systems

• Anatech Instruments • Chemetrix • LabHouse • The Sepsci Group

Pycnometers/density measurements

Photometers

• IMP Scientific & Precision Equipment • Labotec • Poretech

pipettes/diluters

• AE Solutions • Bio-Rad Laboratories • Bruker South Africa • Davies Diagnostics • IMP Scientific & Precision Equipment • PANalytical • The Scientific Group • Thermo Fisher Scientific

• Clean Room Maintenance • Hach South Africa • Hanna Instruments • IMP Scientific & Precision Equipment • Labotec • Merck Pty Ltd • Metrohm SA • The Sepsci Group • Air Filter Maintenance Services • Analytical & Diagnostic Products • Analytical Solutions • Anatech Instruments • Bio-Rad Laboratories • Davies Diagnostics • Labchem • Labotec • Merck Pty Ltd • Metrohm SA • Microsep (Mettler Toledo & Rainin) • The Scientific Group • The Sepsci Group • Thermo Fisher Scientific

Plant growth chambers & rooms

• IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labotec • The Sepsci Group

plastic labware

• Air Filter Maintenance Services • Labchem • Merck Pty Ltd • PA Cuthbert

plastics testing

• IMP Scientific & Precision Equipment • PANalytical

quality control

raman analysers

• Advanced Laboratory Solutions • Bruker South Africa • LabDynamics • Metrohm SA

rapid diagnostic test kits

• Analytical & Diagnostic Products • Bio-Rad Laboratories • Davies Diagnostics • Dehteq (Pty) Ltd • Microsep (Recipe) • The Sepsci Group

reactors

• Celsius Scientific • Hanna Instruments • Labotec • Magna Analytical • Merck Pty Ltd • Microsep (Mettler Toledo) • Poretech

reCIRCULATING COOLERS

• Instrulab - A division of Air & Vacuum Technologies

REFERENCE CULTURES/ QUALITY CONTROL ORGANISMS

• Anatech Instruments • Davies Diagnostics • Industrial Analytical

Product Directory

Refer to Company Directory for contact details

• Quantum Biotechnologies

refractometers

• Hanna Instruments • Labchem • Labotec • Microsep (Mettler Toledo) • Monitoring & Control Labs • Trilab Support cc • Wirsam Scientific & Precision Equipment

repair & maintenance

• AE Solutions • Anacon • Anatech Instruments • IMP Scientific & Precision Equipment • LabDynamics • Labotec • Metrohm SA • The Sepsci Group • Wirsam Scientific & Precision Equipment

Rheometers

• AE Solutions • Advanced Laboratory Solutions • IMP Scientific & Precision Equipment • Micron Scientific • Monitoring & Control Labs

Rotary evaporators

• Anatech Instruments • Celsius Scientific • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labotec • Magna Analytical • Merck Pty Ltd • Monitoring & Control Labs • The Sepsci Group • Trilab Support cc

Safety equipment & accessories

• Merck Pty Ltd

salt spray chambers

• IMP Scientific & Precision Equipment • Labotec

Sample preparation equipment/CONSUMABLES

• Advanced Laboratory Solutions • Analytical & Diagnostic Products • Anatech Instruments • Ilex South Africa • IMP Scientific & Precision Equipment • Industrial Analytical • LabHouse • Labotec • LECO Africa • Magna Analytical • Merck Pty Ltd • Microsep (Waters, Mettler Toledo & Horizon Technology) • National Separations • PANalytical • The Sepsci Group • Wirsam Scientific & Precision Equipment

samplers

• AE Solutions • Anatech Instruments • Hach South Africa • IMP Scientific & Precision Equipment • PA Cuthbert • PANalytical

sensor development

• Bruno Steiner Lab Consultancy (Bio-Logic)

sieves & shakers

• AE Solutions • Air Filter Maintenance Services • Bruno Steiner Lab Consultancy (Kuhner) • Celsius Scientific • Instrulab - A division of Air & Vacuum Technologies • IMP Scientific & Precision Equipment • Labchem • Labotec • Magna Analytical • Merck Pty Ltd • Microsep (Mettler Toledo) • Monitoring & Control Labs • The Scientific Group • Wirsam Scientific & Precision Equipment

soil testing equipment

• AE Solutions • Bruker South Africa • IMP Scientific & Precision Equipment • Labotec • Magna Analytical • Microsep (Waters, Mettler Toledo & Horizon Technology) • Monitoring & Control Labs • Thermo Fisher Scientific

spectrometers

• AE Solutions • Anton Paar Southern Africa • Bruker South Africa • Chemetrix • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labotec • LECO Africa • Merck Pty Ltd • PANalytical • Spectro

• The Sepsci Group • Thermo Fisher Scientific

spectrophotometers

• AE Solutions • Analytical & Diagnostic Products • Analytical Solutions • Anatech Instruments • Chemetrix • Hach South Africa • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labotec • Magna Analytical • Merck Pty Ltd • Monitoring & Control Labs • Shimadzu South Africa • The Sepsci Group • Thermo Fisher Scientific

spectroscopy - accessories

• Anacon • Bruker South Africa • Chemetrix • IMP Scientific & Precision Equipment • Life & Analytical Sciences (Perkin Elmer) • PANalytical • The Sepsci Group • Labotec

spray dryers stirrers

• Anatech Instruments • Hanna Instruments • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labchem • Labotec • LabWorld • Magna Analytical • Merck Pty Ltd • The Sepsci Group • Thermo Fisher Scientific

Surface/surface area analysis

• Advanced Laboratory Solutions • IMP Scientific & Precision Equipment • Labotec • LECO Africa • Microsep (Celsis & Hygiena International) • PANalytical • Poretech • Thermo Fisher Scientific

surface tension

• Advanced Laboratory Solutions • IMP Scientific & Precision Equipment • Micron Scientific

syringes

• Anton Paar Southern Africa • Chemetrix

temperature & humidity

• Archimedes Laboratory Solutions • Hanna Instruments • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Monitoring & Control Labs

Temperature measurement/ thermometers/thermostatS

• Davies Diagnostics • Hanna Instruments • IMP Scientific & Precision Equipment • Labchem • Merck Pty Ltd • Metrohm SA • Monitoring & Control Labs • The Sepsci Group • Trilab Support cc • unitemp

Tensile/compression testing

• Advanced Laboratory Solutions • IMP Scientific & Precision Equipment • Shimadzu South Africa • Wirsam Scientific & Precision Equipment

Textile testing

• Advanced Laboratory Solutions • ATS Laboratory • IMP Scientific & Precision Equipment

Texture analysers

• Advanced Laboratory Solutions • AE Solutions • Bruker South Africa • IMP Scientific & Precision Equipment • Monitoring & Control Labs • PANalytical

Thermal analysis

• Advanced Laboratory Solutions • IMP Scientific & Precision Equipment • Microsep (Mettler Toledo) • Shimadzu South Africa • Thermo Fisher Scientific

thermal desorption

• Advanced Laboratory Solutions • Anatech Instruments • Chemetrix • IMP Scientific & Precision Equipment

• LabHouse • Shimadzu South Africa

THIN LAYER CHROMATOGRAPHY (TLC/HPTLC)

• Bruno Steiner Lab Consultancy (Camag)

tissue culture chambers & rooms

• Air Filter Maintenance Services • IMP Scientific & Precision Equipment

Tissue processors

• IMP Scientific & Precision Equipment • Labotec • The Scientific Group

titrators

• Analytical Solutions • Anatech Instruments • Hach South Africa • Hanna Instruments • Labotec • Metrohm SA • Microsep (Mettler Toledo)

toc analysers

• AE Solutions • Analytical Solutions • Anatech Instruments • Hach South Africa • LabHouse • Labotec • Merck Pty Ltd • Metrohm SA • Microsep (Mettler Toledo & Lighthouse) • Shimadzu South Africa • Thermo Fisher Scientific

Tubing

• Anatech Instruments • ITP Analytical • Labchem • Labotec • National Separations

turbidity

• AE Solutions • Anatech Instruments • Hach South Africa • Hanna Instruments • Labchem • Labotec • Merck Pty Ltd • Microsep (Mettler Toledo)

Turnkey LABORATORY DESIGN

• Air Filter Maintenance Services • IMP Scientific & Precision Equipment • The Sepsci Group • Umoya Laboratory Extraction Technologies

Ultrasonic baths

• AE Solutions • Air Filter Maintenance Services • Celsius Scientific • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labotec • Monitoring & Control Labs • The Scientific Group • The Sepsci Group • Thermo Fisher Scientific

Uv transilluminators

• Analytical & Diagnostic Products • IMP Scientific & Precision Equipment • Labotec • The Scientific Group • The Sepsci Group

Uv/visible spectrophotometry

• Analytical & Diagnostic Products • Analytical Solutions • Anatech Instruments • Chemetrix • Hach South Africa • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labchem • LabDynamics • Labotec • Merck Pty Ltd • Monitoring & Control Labs • Shimadzu South Africa • The Sepsci Group

Vacuum coating

• Labotec • The Scientific Group

Vacuum systems & measurement

• Anton Paar Southern Africa • Chemetrix • Instrulab - A division of Air & Vacuum Technologies • Labotec • Merck Pty Ltd • National Separations • Process Vacuum

Validation

• Air Filter Maintenance Services • Anatech Instruments • Archimedes Laboratory Solutions • Clean Room Maintenance

LMS Issue 3 | 2016

45

• Intertek • LabDynamics • National Separations • PANalytical • Trilab Support cc

Viscometers

• AE Solutions • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labotec • Micron Scientific • Monitoring & Control Labs

Water analysis equipment

• AE Solutions • Analytical & Diagnostic Products • Anatech Instruments • Bruker South Africa • Dehteq (Pty) Ltd • Hach South Africa • IMP Scientific & Precision Equipment • LabHouse • Labotec • LECO Africa • Magna Analytical • Merck Pty Ltd • Metrohm SA • Microsep (Waters & Mettler Toledo) • Monitoring & Control Labs • National Separations

Water baths

• AE Solutions • Air Filter Maintenance Services • Analytical Solutions • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labchem • Labotec • Magna Analytical • Monitoring & Control Labs • The Sepsci Group • Thermo Fisher Scientific

Water distillation/ purification

• Air Filter Maintenance Services • Celsius Scientific • IMP Scientific & Precision Equipment • Instrulab - A division of Air & Vacuum Technologies • Labchem • LabDynamics • Labotec • Merck Pty Ltd • Microsep • National Separations • The Scientific Group • The Sepsci Group • Thermo Fisher Scientific • Trilab Support cc

Water samplers

• Hach South Africa • Labotec • Merck Pty Ltd

Weathering testers

• IMP Scientific & Precision Equipment • Labotec

Western blot kit (See Blot Kits...)

• Anatech Instruments • Bio-Rad Laboratories • Merck Pty Ltd • The Scientific Group • The Sepsci Group

X-RAY analysis

• Bruker South Africa • IMP Scientific & Precision Equipment • Magna Analytical • PANalytical • Shimadzu South Africa

X-ray crystallography

• Bruker South Africa • Magna Analytical • PANalytical

X-ray diffraction

• Bruker South Africa • Magna Analytical • PANalytical • Shimadzu South Africa • Thermo Fisher Scientific • Wirsam Scientific & Precision Equipment

X-ray fluorescence

• Bruker South Africa • IMP Scientific & Precision Equipment • Magna Analytical • Monitoring & Control Labs • PANalytical • Shimadzu South Africa • Spectro • Thermo Fisher Scientific • Wirsam Scientific & Precision Equipment

Zeta potential

• AMS Laboratory Technologies • Analytical Solutions • Micron Scientific • Poretech • SMM Instruments

46

LMS Issue 3 | 2016

Company Directory

Advanced Laboratory Solutions

Afrox

Air Filter Maintenance Services

www.advancedlab.co.za ALS is a leading supplier of laboratory and testing solutions for manufacturing and research organisations in fields covering Metallurgy, Polymers, Coatings, Textiles, Food, Cosmetics, Mineralogy, Paper, Medical and Life Sciences to name a few.

Agents for Agar Scientific, ATM, Gatan, Innovatest. Instron, JEOL, Krüss, Motic, Quorum, TA Instruments, Witec.

www.afrox.com

www.afms.bz AFMS provide certification and supply of Biological Safety Cabinets, Laminar Flow Benches, and Fume Hoods. We supply and install Bsl 3 Laboratories and Bio Containment Facilities and are the authorised dealers for Baker products (www.bakerco.com). We also offer Certification of Clean Rooms and are suppliers of Incubators, Ovens, Water Baths and Circulators.

PO Box 71295 Bryanston 2021

PO Box 5404 Johannesburg 2000

Contact details for all branches (Jhb/CT/Dbn/PE): Tel:

086 0000 ALS (257)

Afrox Special Products is southern Africa’s leading supplier of high purity gases and gas mixtures. Our gases and gas supply equipment are used in applications as diverse as scientific laboratories, refrigeration systems, aerosol propellant products, high technology medical and pharmaceutical processes, environmental emission control, and food processing.

Afrox Special Products supports the need for continuous improvement by developing solution orientated customer partnerships. Our technical and application specialists offer expert advice and training, especially in the safe handling and use of our products. We are committed to quality in supply and administration of a product to suit your requirements, through our numerous branches in SA and 11 other African countries.

Fax: 086 1111 ALS (257)

Afrox Customer Service Centre: 0860 020202 (Ask for Special Products)

PO Box 459 Honeydew 2040 Tel:

Factory 1b 20 Staal Street Kya Sands, Randburg

086-111-2367

Fax: 011-462-1489

Email: info@advancedlab.co.za

Email: customer.service@afrox.linde.com

Email: afms@afms.bz

Anacon

Analytical & Diagnostic Products

Analytical Solutions

www.anacon.co.za

www.adpsa.co.za Marketing and support of analytical, diagnostic and research products, including rapid hygiene monitoring systems; food safety solutions; microplate instrumentation; gel documentation / image analysis systems; gel electrophoresis equipment; radiation monitoring equipment; ion chromatography resins; pipettes; reagents and kits for environmental monitoring, food safety, microbiology, toxicology (animal and forensic drug detection), life science research and phytodiagnostics.

www.analyticalsolutions.co.za Analytical Solutions specialises in the supply of scientific equipment to academic, research and analytical laboratories.

We are agents for Eppendorf, Jasco, Unity Scientific, AMS France, Dani Instruments, SI Analytics, Microtrac, Velp Scientifica, Fedegari autoclaves, Julabo, Ohaus, Schmidt+Haensch and Microfluidics.

Anacon supplies analytical consumables for all major instrument manufacturers and is the sole agent for the PHOTRON range of hollow cathode and deuterium (D2) lamps. Our product range includes: hollow cathode lamps (1.5 and 2”) - coded, uncoded and lumina type; deuterium Lamps for AA, UV, and HPLC detectors; graphite furnace tubes and platforms; and glass or quartz cells (cuvett es).

Anacon also provides support for older generation Varian instruments.

This includes routine and breakdown servicing, operator training, and assistance with sourcing obsolete and scarce spare parts such as keyboards, disk drives, and printed circuit boards.

For more information on our products or our range of pre-owned equipment please check our website.

PO Box 731003 Fairland 2030 Tel:

011-476-5690

180 Kessel Street Fairland 2195

PO Box 6378 Weltevreden Park 1715

Fax: 011-476-7833

Tel:

011-794-5959

Fax: 011-794-5963

PO Box 732 Banbury 2164

370 Angus Crescent, Northlands Business Park, 29 Newmarket Road, Northriding, 2169 Randburg

Gauteng Western Cape KwaZulu Natal Tel: 087-610-0900 Tel: 087-610-1000 Tel: 087-610-1100 Fax: 011-704-7713 Cell: 079-699-7987 Cell: 082-872-3235

Email: Anacon@iafrica.com

Email: support@adpsa.co.za

BAMR

Bio-Rad Laboratories

Biotech Solutions

www.bamr.co.za Quality Control Instruments to ISO

www.bio-rad.com Suppliers of life science and diagnostics equipment and reagents. Products available in the fields of microbiology, serology, virology, haematology, diabetes, autoimmune, drugs, quality control and immunohaematology.

www.biotechsolutions.co.za Biotech Solutions is the authorized partner for Sartorius in South Africa. Conveniently situated in Midrand, Gauteng, we service the whole of South Africa. Please contact us for more information about the complete range of quality Sartorius Laboratory products and Bioprocess solutions.

BAMR is a family run business that supplies, repairs, services and calibrates instruments in the Coatings, Corrosion, Physical Paint Test and Allied Industries since 1946.

Instruments include: Abrasion, Adhesion, Climate, Coating Thickness (Elcometer), Density, Elasticity, Film Applicators, Fineness of Grind, Gloss (Elcometer), Reflectometers, IQ gauges (Rhopoint), Hardness, Moisture (Max Doser, Protimeter, Tramex), Pinhole and Spark, Surface Profiles, Salt, Dust and Cleanliness, Ultrasonic Material Thickness, Viscosity Cups and Rotational Testers

PO Box 23973 Claremont 7735 Tel:

021- 683-2100

Email: sales@bamr.co.za

Email: info@analyticalsolutions.co.za

PO Box 2884 Parklands 2121 Tel:

0861-BioRad (0861-246723) 011-442-8508

Fax: 011-442-8525

Email: southafrica_orders@biorad.com

21 Bavaria Avenue,, Midrand RSA Tel:

011-315-5444

P O Box 6553 House 1685 Fax: 086 477 6390

Email: sales@biotechsolutions.co.za

Company Directory

LMS Issue 3 | 2016

47

High purity reference materials and standards supplier.

Bruker South Africa

Bruno Steiner Lab Consultancy

Bymaz Pty Ltd

www.bruker.com/za Bruker is a leading developer and supplier of innovative analytical instrumentation to the Academic, Research and production environment. The company offers products in the following fields: X-Ray (XRF, XRD and SCD), Infra-Red (FT-IR, Raman, NIR and imaging), Mass Spectrometry (ESI-MS and Maldi-TOF), magnetic spectroscopy (NMR, EPR and pre-clinical MRI), GC-TQ-MS, LC-TQ-MS and microCT.

www.bslc.co.za Bruno Steiner Lab Consultancy is a supplier of the following high technology lab equipment for the sub-saharan countries: • Advion: Compact and affordable high performance mass spectrometer • Bio-Logic: High performance instruments for the research lab - Electrochemistry, Battery & fuel cell, Sensor development and Nanotechnology • Camag: The world leader in Thin Layer Chromatography (TLC) • Kuhner: The world’s most reliable Shaker/Shaking Incubator

PO Box 1091 Cramerview 2060

PO Box 773 Gallo Manor 2052 South Africa

www.bymazpty.co.za Bymaz (Pty) Ltd is a South African based company that imports and distributes Environmental standards in Southern Africa.

• Reference standards for PCBs, PCDDS, Bromide Flame retardants, PFCs and more.

• Organics, Inorganics, Pesticide, Metabolite, Azodye-Dye, Explosives, Phthalate, Vitamin, Personal care products (PPCPs) standards.

EPA Standard mixtures.

Custom solution mixes.

• Certified to ISO 9001:2008, ISO/IEC 17025:2005 and ISO guide 34:2009.

Tel:

011-463-6040

Fax: 011-463-6289

Tel:

011-804-3503

Tel: Fax: 086-719-2778

082-334-4007 011-892-1881

Email: info.za@bruker.com

Email: bruno@bslc.co.za

Email: bymazpty@gmail.com

Carl Zeiss

Cepheid

Chemetrix (Pty) Limited

www.zeiss.co.za Suppliers of complete light and Electron microscopes and imaging solutions for advanced research or routine applications. Bruker Nano agent for AFM and metrology solutions.

www.cepheidinternational.com Cepheid is a leading on-demand molecular diagnostics company that is dedicated to improving healthcare by developing fully-integrated systems and accurate yet easy-to-use molecular tests. The company is focusing on applications where rapid and actionable test results are needed most, in fields such as critical and healthcareassociated infections, virology, sexual health, genetic diseases and cancer.

www.chemetrix.com

PO Box 3003 Randburg 2125 Tel:

011-886-9510 021-551-9202 031-764-1540

Fax: 011-886-9592 021-551-9310 031-764-1562

Famous Grouse House, P O Box 1798 Kildrummy Office Park Lonehill, 2062 Cnr Witkoppen Road & Umhlanga Avenue, Paulshof, 2191

Chemetrix is the sole supplier of Agilent Technologies Chromatography, Mass Spectrometry, and Spectroscopy products for chemical analysis in Southern and East Africa. Chemetrix is also a supplier for Gerstel GmbH and PAC analysers and physical testing products.

Chemical analysis solutions include a comprehensive portfolio of Gas and Liquid Chromatography, Mass Spectrometry and Spectroscopy products, as well as a range of guaranteed analysers covering Food, Environmental, Petroleum, Mining and Forensic applications.

Support, including hardware, software applications and training for these product lines are provided by Chemetrix’s support organisation - a highly technically qualified team of factory trained customer engineers and application chemists.

Tel:

011-541-9801

Fax: 011-541-9802

Service Hotline: 086-009-3477 / 086-00-ZEISS

Tel:

+2711-234-9636

Country-wide support call centre 0860-AGILENT (0860 244 5368)

Email: info.za@zeiss.com

Email: info-rsa@cepheid.com

Email: lynnettel@chemetrix.co.za

Davies Diagnostics

Esco Technologies (Pty) Ltd

Grabner Instruments

www.daviesdiagnostics.co.za We offer EXTRAordinary Service to all laboratories. Our staff is technically qualified to offer full support of all our products.

Our product range covers microbiology, haematology, virology, immunology, serology, blood transfusion, molecular biology, immunohistochemistry and general laboratory products.

www.escoglobal.com Esco Technologies is recognised as a global player in scientific containment, clean air equipment and laboratory equipment technology. The range includes: life science, containment and thermal regulation equipment. Locally based, we offer end-user seminars, training and after-sales support.

For more information regarding our product ranges, please contact us to arrange a visit by one of our sales representatives, or visit our website for product descriptions and DVDs.

www.grabner-instruments.com Grabner Instruments, a subsidiary of AMETEK Inc., is considered one of the world´s leading developers and manufacturers of automatic petroleum testing equipment.

Grabner Instruments’ success is based on the development of portable, rugged and easy-to-operate fuel and oil analysers for accurate quality control in the laboratory, as well as for fast onsite tests in mobile laboratories.

Instruments include vapour pressure, flash point, fuel analysis, distillation, viscosity and water content.

PO Box 3222 Randburg 2125 Tel:

Local: 011-777-7600 International: +27 11 777-7600

Email: mkt@daviesdiag.co.za

Fax: Local: 011-787-6917 International: +27 11 787-6917

Postnet Suite 37 Kyalami Private Bag X11 Halfway House Tel:

011-314-3184

Email: admin@escosa.co.za sales@escosa.co.za

Unit 2 Landsmark Park 17 Landmarks Avenue Kosmosdal Ext. 11 Centurion

58 Maple Road Pomona Kempton Park Tel:

011-979-4241

Fax: 011-979-3564

Email: Marie.Julsing@Ametek.com

48

LMS Issue 3 | 2016

Company Directory

Hanna Instruments

Heraeus South Africa (Pty) Ltd

Industrial Analytical

www.hanna.co.za Hanna Instruments produce the following products: Chemical Test Kits, pH, Conductivity, TDS, ISE, Titration, Dissolved Oxygen, Multi-Parameters Meters, Magnetic Stirrers, Photometers, Chemical Oxygen Demand, Turbidity, Refractometers, Temperature, Relative Humidity, Lux, Process Instrumentation and Dosing Pumps.

www.heraeus.com | http://heraeus-pt-labware.com

www.industrialanalytical.co.za | www.lgcstandards.com

Heraeus South Africa (Pty) Ltd is a subsidiary of Heraeus Holding GmbH, the global precious metal and technology firm. Our range of Labware includes crucibles, LOI dishes, XRF crucibles and moulds , electrodes, parting trays, tongs, wires, etc. in Platinum, Iridium, Gold , Rhodium, and these alloys. Our services include metal exchange, Scrap reimbursement and refining.

We also supply Precious Colour Decorations, Thin film sensors, Thermocouple wire, quartz glass and Bonding wire.

Your trusted source for 26 years!

Industrial Analytical (Pty) Ltd., an ISO 9001:2008 certified and BEE rated company, specialises in the supply of certified reference materials/reference standards, certified solution standards for ICP/AAS, fine chemicals (high purity metals/compounds and HPLC solvents) and Claisse fusion equipment.

ISO Certificates of Analysis (COA), with guaranteed purities (up to 99.999999% pure) and MSDS supplied with our products.

Tel:

011- 615-6076 (JHB) 031-701-02711 (DBN) 021-946-1722 (CT)

Email: hanna@hanna.co.za durban@hanna.co.za ct@hanna.co.za

Fax: 011-616-0387 031-701-2706 021-946-1723

29 B Atlas Road, Anderbolt, Boksburg South Africa

(JHB) (DBN) (CT)

Tel:

+27-11-918-4374 +27-82-901-2072

PO Box 6089 Dunswart 1508 Fax: 011-894-7730

PO Box 12897 Vorna Valley 1686 Tel:

011-466-4321

Fax: 011-466-4611

Email: ashwin.simay@heraeus.com

Email: info@industrialanalytical.co.za

Intertek

LabDynamics

LabHouse

www.intertek.com

www.labdynamics.co.za

Intertek is a leading provider of independent analytical services.

Their laboratories are staffed by trained professionals with years of industry knowledge and expertise. They provide testing services throughout sub-Saharan Africa for a broad range of industries, including environmental, minerals, petroleum, chemical, oil, soil and food. Intertek delivers the crucial laboratory support you need to meet your product research, development, and quality goals.

LabDynamics supplies quality laboratory equipment, consumables, analytical training courses and service support in South Africa and southern Africa.

Our product range includes: dissolution baths, disintegration testers, handheld Raman analysers, UV-Vis spectrophotometers, HPLC systems, chromatography data systems and laboratory water systems.

We provide an all-vendor validation service for spectrophotometers and chromatography systems.

www.LabHouse.co.za LabHouse is a scientific business specialising in high-tech equipment and professional lab training for chemical industries and scientific research. Agents for market leading Tekmar TOC and VOC systems, Scion GC and GC-MS, and Entech Thermal Desorption. Providers of top HPLC/GC training courses and Lab Management related training sessions.

Charter House, 13 Brand Road, Glenwood, Durban 4001 Tel:

031-274-8000 (Lubricants, Microbiology, Validation) 031466-3344 (Petroleum) 011-552-8149 (Oil Testing & Environmental) 011-396-1101 (Laboratory & GMP Training) 011-964-1004 (Agri & Food Testing) 011-552-8149 (Calibration)

PO Box 344 Cramerview 2060

P O Box 1016 Paardekraal 1752 Tel:

011-792-4262

011-465-4405

Fax: 086-672-0980

Tel:

Cell: 082-498-1111

Email: sales@LabHouse.co.za

Fax: 011-465-4674

Email: info.africa@intertek.com

Email: labdynamics@mweb.co.za

Labotec Pty (Ltd)

Lasec SA

LECO Africa

www.labotec.co.za Labotec supplies laboratory equipment and analytical instruments to sub-Sahara Africa. Established in 1960 as one of the first lab supply companies in SA, we have partnered ourselves to represent some of the worldâ&#x20AC;&#x2122;s leading brands, bringing to the local market the best quality the world has to offer in diverse fields from medical, food, environmental, industry, chemical to pharmaceuticals. In addition, Labotec Technical Services offer a broad repair and maintenance programme with personalised support.

www.lasecsa.co.za Lasec is an African supplier of many internationally recognised brands of scientific and laboratory equipment.

We take pride in positioning ourselves as a partner to our clients assisting them in achieving optimal precision, performance and results. At Lasec we strive to be Trustworthy, Transparent, Dynamic, Professional and Excellent in everything we do.

www.lecoafrica.co.za LECO Africa is a wholly-owned subsidiary of the privatelyowned company LECO Corporation, USA, servicing the South African Analytical and Metallographic industries since 1976.

We provide LECO Analytical, Metallographic, Spectrographic and Separation Science equipment, Consumables and related spare parts manufactured and assembled in the USA. LECO Africa serves the entire sub-Saharan region, with offices in Gauteng, Western Cape, Mpumalanga and KwaZulu Natal, and distributors in the Eastern Cape, Namibia, Ghana, Kenya, Tanzania, Uganda and Zambia.

PO Box 6553 Halfway House, Midrand 1685 Tel:

011-315-5434 021-531-7660 031-566-4870

Email: sales@labotec.co.za

Fax: 011-315-5882

52 Old Mill Road Ndabeni 7405 Tel:

021-531-7504

Email: sales@lasec.co.za

Fax: 021-531-7562 Tel:

011-974-1681

Fax: 011-974-1848

Company Directory

LMS Issue 3 | 2016

LimsTricS

Merck Pty Ltd

Metrohm SA Pty (Ltd)

www.limstrics.co.za

www.merckmillipore.com Merck is an innovation-driven life science company offering a broad spectrum of proven tools and technologies, together with performance solutions that help our customers succeed in the research, development and production of biotechnology and pharmaceutical drug therapies.

www.metrohm.co.za

PO Box 1998 Halfway House 1685

Postnet Suite 587 Private Bag x29 Gallo Manor, 2052

LimsTricS is the Authorised Distributor and Strategic Implementation Partner for Thermo Fisher Scientific Informatics Division in Southern Africa. LimsTricS has extensive experience in the rollout and support of Laboratory Information Management Systems (LIMS) with SampleManager LIMS as the flagship LIMS product.

Tel:

+27-12-809-2833

086-006-3725

Fax: 086-052-2329

49

Metrohm SA is the sole agent for the following Metrohm products: • Titration, pH and conductivity, Karl Fischer, ion chromatography and voltammetry • Autolab potentiostats • Applikon online process analysers including, TOC • Near-infrared systems for lab and process • MIRA - portable Raman analyser.

Our products are widely used in industries such as environmental, pharmaceutical, food, mining, electroplating, petrochemical and tertiary institutions for teaching/research.

011-656-1918

Fax: 011-656-2698

Mobile: +27-82-334-6573

Tel:

Email: support@limstrics.co.za

Email: labsupply@merck.co.za

Email: info@metrohm.co.za

Microsep (Pty) Ltd

Monitoring & Control Laboratories

The National Laboratory Association

www.microsep.co.za

www.moncon.co.za Monitoring and Control is a leading supplier of laboratory instrumentation and chemicals. Brands include Brookfield, Binder, Retsch, YSI, Grant Instruments, Buchiglasuster, Atago, VWR, BDH, Stanhope-Seta, Kittiwake, Normalab, IKA, Pilodist, Dueperthal, Huber and more. The Company has Service Workshops & Sanas Labs for Viscosity & Temperature Calibrations at its Johannesburg and Durban offices.

2 Saturn Crescent, Linbro Business Park, Frankenwald Ext. 30, Sandton, 2196

No 10, Village Crescent Linbro Business Park 2065 Gauteng, South Africa

PO Box 890226 Lyndhurst 2106

www.nla.org.za The National Laboratory Association - South Africa (NLA-SA) is the premier laboratory association in SA representing over 200 testing and calibration laboratories, with the large majority being SANAS accredited facilities. Providing support for the laboratories is the main goal of the NLA-SA and the main services that are offered are the following: 1. Training for testing and calibration laboratory staff 2. Proficiency testing schemes 3. Professional registration for metrologists 4. Annual Conference / Workshop

Tel: JHB: CT: DBN:

Fax: 011-608-4741 086-010-9258 086-516-1141

Microsep is a supplier of high technology scientific, analytical, laboratory and weighing instrumentation in Southern Africa.

Our focus is to provide high quality scientific products and innovative solutions to satisfy the needs of our customers in the general laboratory, pharmaceutical, biotechnology, food and beverage, chemical and petro-chemical, environmental and industrial markets. Market leading brands include Waters Chromatography and Mettler Toledo.

Tel:

011-553-2300 021-914-0393 021-886-9157 031-701-4705/6 041-365 5168

Email: info@microsep.co.za

Fax: 011-553-2400

011-608-4664 086-010-9259 031-914-2912

Tel:

PO Box 298 Persequor Park, Pretoria 0020 Tel:

012-349-1500

Fax:

012-349-1501

Email: sales@moncon.co.za / exports@moncon.co.za

Email: steves@nla.org.za

National Separations

PANalytical Pty (Ltd)

www.natsep.com National Separations (NATSEP) is the supplier of laboratory filtration, water purification and consumable products for the pharmaceutical, biotechnology, food & beverage and industrial industries. NATSEP, a subsidiary of Microsep (Pty) Ltd, is the official Southern African distributor for Sartorius Stedim Biotech, Albet and Amazon Filters.

NATSEP provides the most cost effective filtration solution, by combining excellent products with years of filtration expertise and exceptional service.

www.panalytical.com The Analytical X-ray Company that provides the total X-ray solution, from fundamental research to industrial production control by means of the latest innovative technology in X-ray diffraction and X-ray fluorescence spectrometry. Using the best XRF specialists in South Africa, PANalytical offers XRF training courses. The courses are designed for basic operators and lab managers, and can either be presented at the customer’s site or at the PANalytical offices in Ferndale, Randburg.

Tel:

Jhb: 011-553-2300 CT: 021-914-0393 CT: 021-886-9157 DBN: 031-701-4705/6 PE: 041-365-5168

Fax: 011-553-2400 021-886-9078

Email: salesjhb@natsep.co.za / info@microsep.co.za

Private Bag 4015 Ferndale 2160 Tel:

011-577-0880

Fax: 011-577-0882

Email: wynand.smit@panalytical.com

50

LMS Issue 3 | 2016

Company Directory

Polimatrix SA

Scientific Supply Services (SSS)

Separation Scientific SA

www.polimatrix.co.za Polimatrix SA designs and manufactures a full range of laboratory furniture and equipment specifically for use in nonmetallic clean labs, wet chemical and special purpose labs.

All furniture and equipment are manufactured using specialised thermoplastic materials.

Polimatrix SA specialises in fans, fume cabinets, laminar flow cabinets, cupboards, worktops, corrosive chemical storage, acid scrubbers and ducting.

www.ssscc.co.za

www.sepsci.co.za Separation Scientific SA (Pty) Ltd is a South African based import and distribution company of scientific equipment and reagents. We cater for the needs of three main fields of interest: Industry, Clinical/Diagnostics/Biochemistry and Academic Research.

Members of the SepSci group.

PO Box 1402 Noordheuwel 1756

PO Box 73876 Fairland 2030

Tel:

1047 Katrol Avenue Robertville 1709

00 27 11 672-2101

Fax: 00 27 11 672-8626

SSS is a highly specialised company dedicated to offering superior support in niche markets in Chromatography and related disciplines. SSS is an OEM distributor for Vici and an accredited distributor for all Varian consumables; Vici Valves and GC Detectors; Precision Sampling Syringes; ChromPerfect Data Systems; Hamilton Syringes; SGE Syringes & Supplies; National Scientific Vials, Septa, Syringe Filters, Caps etc; Bohlender Teflon Products; GL Science Leak Detectors; Specialised and Refurbished Instruments; Capillary Columns; Solid Phase Extraction; Nikepal Syringes. Training is a priority at SSS and regular hands-on courses are offered. The range is expanding annually and SSS also manufactures specialised Inlet Systems for sample introduction of gases and semi-gaseous mixtures.

Tel:

011-476-8065/8357

15 Catherine Avenue MISA Park Northcliff, 2195

PO Box 4328 Honeydew 2040

Fax: 086-648-1862

Tel:

011-794-4117

Fax: 011-794-2160

Email: jacques@polimatrix.co.za

Email: ssscc@netactive.co.za

Email: info@sepsci.co.za

Shimadzu SA (Pty) Ltd

Skylabs

Spectro

www.shimadzu.co.za Shimadzu SA (Pty) Ltd. offers direct sales, service and support to customers in the Southern African Region for all of their analytical and measuring instruments including instruments for chromatography, massspectrometry, spectroscopy, elemental analysis, surface analysis, materials testing, life scienice research, weight measurement, thermal analysis, particle size analysis and TOC analysis.

www.skylabs.edx.co.za Skylabs is suppliers and manufacturers of all kinds of scientific, Chemicals Acids and Bases; dehydrated culture media, Biological Buffers; General Reagents, Research Chemicals and Solvents, Laboratory Glassware, Instrumentation, Medical Consumables and Instrumentation. Providing quality instruments and related accessories; at extremely competitive prices and within the scheduled time is the main characteristic of our group. Our products now reach all over the globe. The precision quality of our products is of International Standards.

www.spectro.com Spectro is one of the worldwide leading suppliers of analytical instruments for optical emission and X-ray fluorescence spectrometry. As a member of the AMETEK materials analysis division, Spectro manufactures advanced instruments, develops the best solutions for strongly varying applications and provides exemplary customer service. Innovation, instrument concerns and customer relations are its main activities. Instruments include, ICP, ICP-MS, EDX-Ray, handheld X-ray, portable X-ray- SCOUT, mobile and stationary Arc and Spark OES.

PO Box 384 Welobie 1714 Tel:

011-795-2608

Wild Fig Business Park Units 37-40 1494 Cranberry Street Honeydew Roodepoort 2170

PO Box 57003 Springfield, Johannesburg 2137 Tel:

Fax: 011-795-1493

011-434-2832 083-513-1751

6-10 Hippo Road Springfield Fax: 086-670-1774

Tel:

Email: sales@skylabslns.co.za

Email: info@shimadzu.co.za

58 Maple Road Pomona Kempton Park 011-979-4241

Fax: 011-979-3564

Email: mjulsing@spectro.org.za

The world leader in serving science Technology Systems Integration (Pty) Ltd www.labinfo.co.za LabInfo is the versatile and affordable laboratory information management system (LIMS) from Technology Systems Integration (Pty) Ltd (TSI).

Powerful reporting and instrument integration capabilities save time and improve accuracy in the lab, while access control and traceability functions assist with regulatory requirements.

LabInfo uses Internet technology, enabling quick and easy access from any PC with a web browser. Built-in remote support and data backup facilities ensure maximum uptime of the system. A variety of industries are using LabInfo and since it is locally developed, it can be cost-effectively customised to suit your needs.

Tel:

+27-11-792-1094

Email: labinfo@tsi.co.za

Fax: +27-11-793-6523

The Scientific Group

Thermo Fisher Scientific

www.scientificgroup.com The Scientific Group is a Clinical Diagnostic and Life Science company with 32 yearsâ&#x20AC;&#x2122; experience in sales and service in the science and healthcare industry. We supply, support and service diagnostic laboratory equipment and consumables to pathology laboratories and provide research institutions and science companies with a comprehensive range of high-tech equipment & associated consumables as well general lab reagents and consumables.

www.thermo.com Thermo Fisher Scientific is the largest supplier of analytical instruments in the world. The established office in South Africa offers sales and service back-up to the southern Africa market. The products we offer are: AA, OES, ICP, ICP-MS, LC, GC, LCMS, GCMS, FTIR, UV, XRD, XRF, Surface Analyses, Automation.

Scientific Group House Cosmo Business Park Malibongwe Drive, Randburg 2188 Tel:

Jhb: CT: Dbn: Bloem:

087-353-5600 087-353-5800 087-353-5900 087-353-0800

PO Box 13119 Vorna Valley 1686

Fax: Jhb: CT: Dbn: Bloem:

086-557-2874 086-500-7313 086-743-2791 086-743-6107

PO Box 557 Kempton Park 1620 Tel:

011-776-0000

Fax: 011-822-3948

Email: analyze.sa@thermo.com

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