Phyllanthi amari Herba awmifqD;jzL (Taun zi: bju) 93

Myanmar Herbal Pharmacopoeia Volume II of 10 mL of distilled water and shaken vigorously for a few minutes, a long lasting foam is produced.

E. A test tube containing 70% ethanolic extract of the drug is added 5-10 drops of dilute hydrochloric acid followed by a small pieces of magnesium ribbon. Boil solution for a few minutes, pink colour is formed.

F. Add 10 mL of chloroform to 1 g of the drug for 6 hours and filter. The filtrate is added to 0.3 mL of acetic anhydride followed by a few drops of concentrated sulphuric acid. A brick red colour is formed.

G. The aqueous extract of the drug is treated with a few drops of sodium hydroxide solution. A yellow colour is appeared in the test tube.

TLC analysis

To extract 1 g of powder drug add 15 mL of ethyl acetate on the water bath for one hour and filter. The filterate is evaporated to dryness. The dried extract is redissolved in 1 mL of ethyl acetate for Thin Layer Chromatography. Application volume : 15 mL Developing solvent system : Hexane : Ethyl acetate (7:1.4) Stationary phase : Silica gel GF254 Aluminium sheet Spray reagent : Vanillin in sulphuric acid

Fig. 2 Thin-layer chromatogram of Ethyl acetate extract of the dried barks of Alstonia scholaris (L.) R. Br.