APPENDIX

Myanmar Herbal Pharmacopoeia Volume II 135

APPENDIX

Preparation for Spray Reagents

1. Anisaldehyde-sulphuric acid reagent

Anisaldehyde (0.5 mL) is mixed with 10 mL glacial acetic acid, followed by 85 mL methanol and 5 mL concentrated sulphuric acid. Spray the plate with about 10 mL and heat at 105 °C for 5-10 minutes. Evaluate in day light or in UV 365 nm light. Reagent must be freshly prepared.

2. Ninhydrin reagent (NIH)

30 mg ninhydrin is desolved in 10 mL n-butanol, followed by 0.3 ml 98% acetic acid. After spraying (8-10 mL), the plate is heated for 5-10 minutes under observation and evaluated in visible.

3. Ferric chloride solution, ethanol

A 10 % w/v solution of ferric chloride in ethnol.

4. Ethanolic sulphuric acid

Prepare a solution of 10-20 % v/v of sulphuric acid in ethanol by adding the acid dropwise to ethanol with shaking. Spray the plate with about 10 mL and heat at 105 °C for 5-10 minutes.

5. Potassium hydroxide reagent

Prepare a solution of 5-10 % ethanolic potassium hydroxide. The plate is sprayed with 10 mL and evaluated in day light or in UV 365 nm, with or without warming.

6. Vanillin-sulphuric acid reagent

1 % ethanolic vanillin (solution 1) 10 % ethanolic sulphuric acid (solution 2) The plate is sprayed with 10 mL solution 1. followed immediately by 10 mL solution 2. Heat at 110 °C for 5-10 minutes under observation. Evaluate in day light.