SILICA GEL COLUMN CHROMATOGRAPHY
SILICA GEL COLUMN CHROMATOGRAPHY Column chromatography is used for separating and purifying the liquids and solid compounds. It is an effective chromatography technique in which stationary phase adsorbs the unwanted compounds and other impurities using silica gel and activated aluminum oxide powder as powerful adsorbents
Benefits of Silica Gel Column Chromatography • Optimize separation efficiency • High adsorption capacity with large surface area • Reproducible performance • Suitable for large scale applications • Excellent flow rates • Direct scalability
General uses of chromatography in our real life are •
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Pharmaceutical Company –determine amount of each chemical found in new product. Hospital –detect blood or alcohol levels in a patient’s blood stream. Law Enforcement – to compare a sample found at a crime scene to samples from suspects. Environmental Agency – determine the level of pollutants in the water supply. Manufacturing Plant – to purify a chemical needed to make a product. Biotechnology industry – establishing the purity or concentration of compounds in biotechnological research. Biological application – Chromatography has many applications in biology. It is used to separate and identify amino acids, carbohydrates, fatty acids, and other natural substances. Environmental testing laboratories use chromatography to identify trace quantities of contaminants such as PCBs in waste oil and pesticides such as DDT in groundwater.
Application of Column Chromatography •
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Analytical uses: For analytical purposes, column chromatography finds limited applications. Vestergaard and Sayegh could separate seven urinary steroids within 5 hours which requires 36 hours on a normal column. They have used narrow Teflon tubing packed with aluminum oxide or silica gel. Separation of geometrical isomers: The separation of cis/trans isomer is based on the steric factors. Isomers whose functional groups can approach the surface of the adsorbent more easily are more strongly adsorbed. Separation of Diastereomers Separation of tautomeric mixtures. Separation of racemates.
Types of silica gel for column chromatography mesh size. SILICA GEL 60-120 MESH Related Categories:
Flash Chromatography, TLC
Grade:
Laboratory Grade
Particle Size:
60-120 Mesh (250125μm)
Boiling Point:
2230 0C
Melting Point:
>1600 0C
Bulk Density KG/LIT
500-650
Ph 10% aq solution
7 ± 0.5
Fe (Iron)
<75 ppm
Chloride(NaCl)
Max 300ppm
Pore Diameter –A
40-60A
Surface Area m2/gm
350-450
Moisture Content %
<6.00
SILICA GEL 60-200 MESH Related Categories:
Flash Chromatography, TLC
Grade:
Laboratory Grade
Particle Size:
60-200 Mesh (25074μm)
Boiling Point:
2230 0C
Melting Point:
>1600 0C
Bulk Density KG/LIT
545-620
Ph 10% aq solution
7 ± 0.5
Fe (Iron)
<75 ppm
Chloride(NaCl)
Max 300ppm
Pore Diameter –A
40-60A
Surface Area m2/gm
350-450
Moisture Content %
<5.00
SILICA GEL 70-230 MESH Related Categories:
Flash Chromatography, TLC
Grade:
Laboratory Grade
Particle Size:
70-230 Mesh (21063μm)
Boiling Point:
2230 0C
Melting Point:
>1600 0C
Bulk Density KG/LIT
500-650
Ph 10% aq solution
7 ± 0.5
Fe (Iron)
<75 ppm
Chloride(NaCl)
Max 300ppm
Pore Diameter –A
40-60A
Surface Area m2/gm
350-550
Moisture Content %
<5.00
SILICA GEL 100-200 MESH Related Categories:
Flash Chromatography, TLC
Grade:
Laboratory Grade
Particle Size:
100-200 Mesh (14974μm))
Boiling Point:
2230 0C
Melting Point:
>1600 0C
Bulk Density KG/LIT
500-650
Ph 10% aq solution
7 ± 0.5
Fe (Iron)
<75 ppm
Chloride(NaCl)
Max 300ppm
Pore Diameter –A
40-60A
Surface Area m2/gm
350-550
Moisture Content %
<6.00
SILICA GEL 200-400 MESH Related Categories:
Flash Chromatography, TLC
Grade:
Laboratory Grade
Particle Size:
200-400 Mesh (7437μm)
Boiling Point:
2230 0C
Melting Point:
>1600 0C
Bulk Density KG/LIT
500-650
Ph 10% aq solution
7 ± 0.5
Fe (Iron)
<65 ppm
Chloride(NaCl)
Max 300ppm
Pore Diameter –A
40-60A
Surface Area m2/gm
450-650
Moisture Content %
<5.00
SILICA GEL 230-400 MESH Related Categories:
Flash Chromatography, TLC
Grade:
Laboratory Grade
Particle Size:
230-400 Mesh (6337μm)
Boiling Point:
2230 0C
Melting Point:
>1600 0C
Bulk Density KG/LIT
400-650
Ph 10% aq solution
7 ± 0.5
Fe (Iron)
<60 ppm
Chloride(NaCl)
Max 300ppm
Pore Diameter –A
40-60A
Surface Area m2/gm
450-650
Moisture Content %
<5.00
SILICA GEL 400-800 MESH Related Categories:
Flash Chromatography, TLC
Grade:
Laboratory Grade
Particle Size:
400-800 Mesh (3718μm)
Boiling Point:
2230 0C
Melting Point:
>1600 0C
Bulk Density KG/LIT
400-650
Ph 10% aq solution
7 ± 0.5
Fe (Iron)
<60 ppm
Chloride(NaCl)
Max 300ppm
Pore Diameter –A
55-60A
Surface Area m2/gm
450-650
Moisture Content %
<5.00
Reference Link • http://column-chromatography.com/chromatography-adsorbents/ • http://column-chromatography.com/thin-layer-chromatography-2/ • http://column-chromatography.com/silica-gel-column-chromatography/ • http://column-chromatography.com/silica-gel-100-200-mesh/ • http://column-chromatography.com/silica-gel-230-400-mesh/
• http://column-chromatography.com/aluminium-oxide-neutral/ • http://column-chromatography.com/aluminium-oxide-basic/ • http://column-chromatography.com/flash-chromatography-3/ • http://column-chromatography.com/blog/ • http://column-chromatography.com/silica-gel-gf/ • http://column-chromatography.com/silica-gel-60-120-mesh/ • http://column-chromatography.com/silica-gel-powder/
• http://column-chromatography.com/aluminium-oxide-chromatography/ • http://column-chromatography.com/extraction-of-bio-active-compounds-from-medicinal-plants/
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