FREE RADICALS EFFECT Respiration in the presence of oxygen is essential for our organism’s cellular life. As a result of this process, molecules called free radicals are produced which, throughout our life, have negative effects on our health. Free radicals are capable of altering DNA (genes), proteins and lipids or fats (oxidation). To keep healthy, there should be a fine balance between the mechanisms that generate free radicals and those that neutralize them. In many tissues, the reserves of antioxidation agents are limited, so an increase in the production of free radicals or a weakening of the antioxidant defences may lead the organism to suffer oxidative stress and cell damage. From this point of view, “oxidative stress” means a change in the balance – between oxidants and antioxidants – in favour of the first. Oxidative stress is the cause of wrinkles, flaccidity and age spots. The skin’s vital cells need to be protected by antioxidants. This is the reason why the incorporation of antioxidants such as Hydroxytyrosol in skincare products is so important.
OLIVE POLYPHENOLS Polyphenols are bioactive substances which are widely distributed throughout the vegetable kingdom and are elements which are actively present in Mediterranean Diet, along with other phytonutrients. The polyphenolic compounds are characteristic constituents of the olive, with biological properties such as antioxidant activity, the ability to capturate oxygenated and/or electrophilic species (free radicals) and inhibit nitrosation and chelate metal ions.
HYDROXYTYROSOL is a polyphenol from the olive . It stands out as the most powerful. Chemical Structure
R3 = OH Hydroxytyrosol (3,4 – dihydroxyphenylethanol DPE)
MECHANISM OF ACTION
It is effective at all levels: Hydroxytyrosol protects against both environmental and internal agents, protecting cell membranes and preventing cellular and structural disruption, desquamation, dehydration and aging.
Olive Active Hidroxitirosol
HIGHTLIGHTS
A VERY COMPLETE ANTIOXIDANT
Electron scavenger: Our DPPH and ORAC studies show that OA Hidroxitirosol 40% is much superior than Q10, Idebenonae, Vit E, Vit C and many other usual antioxidants.
Cell and DNA protector: OA Hidroxitirosol 40% is present in cells it has a cellular antioxidant activity 1.5 times TE (50% higher than Vit E).
Stable: Hydroxytyrosol has the same antioxidant capacity as Vitamin C but with greater stability as Vitamin E.
Protector of lipid peroxidation: Our studies demonstrate that a dose 10 times lower, OA Hidroxitirosol 40% is 30% more effective than Vitamin E in protecting skin lipoperoxidation.
Whitening effect (Patented): Our in vitro and in vivo tests show that OA Hidroxitirosol 40% is more efficient than Arbutin, Kojic Acid and Vitamin C.
Soothing and repairing effect on skin: Compared to formulations containing Vitamin E, OA Hidroxitirosol 40% is able to double the efficacy of skin damaged recovery.
SPF booster: Our in vitro and in vivo studies show that increases by 20% a sunscreen’s SPF. Furthermore we have demonstrated that pre-treatment with OA Hidroxitirosol improve by 150% protection against solar radiation.
Metal ion chelator
Encapsulated LPD: Olive Active has the first hydroxytyrosol ready to be incorporated in cosmetic formulations avoiding colour or odour impact and boosting efectiveness.
Encapsulated HMA: Olive Active has the first hydroxytyrosol ready to be incorporated in hair care formulations.
IN VITRO - DPPH ASSAY 1 IMMEDIATE READING
Hydroxytyrosol has the same antioxidant capacity as Vitamin C but with greater stability. Much higher than Q 10 and Idebenone.
IN VITRO – CELLULAR ANTIOXIDANT Determination of the effect of hydroxytyrosol in cellular antioxidant activity by measuring its ability to prevent the formation of the peroxyl radical.
When OA Hidroxitirosol 40% is present in cells it has a cellular antioxidant activity 1.5 times TE (50% higher than Vit E).
TEST IN VIVO EVALUATION OF ANTIOXIDANT ACTIVITY OF A COSMETIC PRODUCT THROUGH THE DETERMINATIONS OF LIPID PEROXIDATION PRODUCTS (LPO)
% of reduction LPO T7 vs T0
Olive Active HT 0.2% is more effective than VitE 2%
MECHANISM OF ACTION Hidroxitirosol acts in two ways:
- Inhibits melanin production - Increases the amount of glutathione (+ phaeomelanin)
TEST IN VITRO - Evaluation of depigmenting activity on Melanocytes Cultures OLIVE ACTIVE HIDROXITIROSOL 40% vs. COMPETITORS Method: Melanocyte cultures are treated with different whitening actives: VitC, Arbutin, Kojic Acid and Olive Active Hidroxitirosol.
TEST IN VIVO - Evaluation of depigmenting activity on hyperpigmented spots OLIVE ACTIVE HIDROXITIROSOL 40% vs. VITAMIN C Instrumental Evaluation:
Results:
20 volunteers in which three skin spots are selected and treated with different formulations. Products applied twice a day during 56 days. Measured with MEXAMETER HX18.
Up to 24% of improvement. All
Up to 32% of improvement.
All panelists improve over 12%.
panelists without exception presented a reduction of melanin.
Average of 12% statistically significant.
Average of 18% statistically significant.
Comparative with Vitamin C: • Results of OA Hidroxitirosol 40% are 50% higher at 28 days and 20% higher at 56 days. • Dosification of OA Hidroxitirosol 40% is 10 times lower than Vitamin C.
Photographs of selected spots: OA HIDROXITIROSOL 40% AT 0.2%
Results at t28 and t56 are statistically significant.
IN VIVO - EVALUATION OF THE SOOTHING/REPAIRING Redness is measured with MEXAMETER MX18. TEWL is measured with TEWAMETER TM300.
Photoirradiation is 1,5x volunteers’ MED , it is induced by a solar simulator (UVA+UVB) during 90 seconds.
Redness/Erythema
TEWL
Index repair 30’=1-(t30’t0)/(t0’-t0) Index repair 4h=1-(t4h-t0)/(t0’-t0)
OLIVE ACTIVE HT 0,2% IS THE MOST EFFECTIVE TREATMENT
IN VITRO Increase of SPF in a formulation
SUNSCREEN
SUNSCREEN + 0.2% OLIVE ACTIVE HIDROXITIROSOL
17.6
20.4
Method: In vitro determination of Sun Protection Factor (SPF) ISO 24443:2012.
1- Increases by 16% the SPF of the sunscreen. 2- Prevents sunscreens degradation.
SPF IN VITRO
IN VIVO Increase of SPF in a formulation Method: In vivo determination of the solar protection factor (SPF) ISO 24444:2010 (Results equivalents to (SPF) test method Colipa 2006).
1- Increases by 19,5% the SPF of the sunscreen. 2- Prevents sunscreens degradation.
IN VIVO - EVALUATION OF THE PROTECTIVE EFFECT Redness is measured with MEXAMETER MX18. TEWL is measured with TEWAMETER TM300.
Photoirradiation is 1,5x volunteers’ MED , it is induced by a solar simulator (UVA+UVB) during 90 seconds.
Redness/Erythema
TEWL
PRODUCT RANGE Olive Active Hidroxitirosol 40%: Olive fruit natural extract with 40% hydroxytyrosol. Olive Active Hidroxitirosol LPD: OA Hidroxitirosol 40% encapsulated in a phospholipid device. Olive Active Hidroxitirosol HMA: OA Hidroxitirosol 40% encapsulated specially for the hair.
O. A HIDROXITIROSOL LPD The LPD´s consist of vesicles, mainly made of phospholipids of natural origin. These phospholipids are organized in bilayers allowing the integration of active ingredients within its structure. IN VIVO TEST Redness is measured with MEXAMETER MX18. TEWL is measured with TEWAMETER TM300.
Photoirradiation is 1,5x volunteers’ MED , it is induced by a solar simulator (UVA+UVB) during 90 seconds.
Redness/erythema
TEWL Index repair erythema Index repair erythema 30‘ = 1-(t30’-t0)/(t0’-t0) 4h= 1-(t4h-t0)/(t0’-t0)
CONTROL
0,19
Index repair TEWL Index repair TEWL 30'= 1-(t30’-t0)/(t0’-t0) 4h= 1-(t4h-t0)/(t0’-t0)
7,95
CONTROL
0,39
10,39
MOISTURIZING CREAM OA HT LPD (2%)
21,75 60,23
66,02 91,56
OA HT 40% (0,2%)
52,28
80,83
MOISTURIZING CREAM
8,83
34,98
OA HT LPD (2%) OA HT 40% (0,2%)
48,52 43,89
91,03 67,09
100
100
90
90
80
80
70
CONTROL
60 MOISTURIZING CREAM
50
70
CONTROL
60
MOISTURIZING CREAM
50
40
OA HT LPD (2%)
40
30
OA HT 40% (0,2%)
30
20
20
10
10
0 Index repair erythema 30'
Index repair erythema 4h
OA HT LPD (2%) OA HT 40% (0,2%)
0 Index repair TEWL30'
Index repair TEWL 4h
Increases biodisponibility
Facilitates the formulation
Without colour incidence
Without odour incidence
Thanks to this encapsulation, formulate with hydroxytyrosol is easy and the efficacy is boosted.
O. A HIDROXITIROSOL HMA Strong antioxidant + strong adhesion to the fiber THE BEST OPTION FOR HAIR PROTECTION
HMA TECHNOLOGY: HAIR MICRO ADHESIVE
Positively charged coated microparticules. Strong adhesion to hair (negative charge).
Active steadily released. Coacervation microencapsulation
Substantivity on hair tested.
COSMETIC APPLICATIONS • Antioxidant Treatments • Antiageing Treatments • Protector day creams or makeup • BB or CC creams • Sunscreen Complex • Whitening/ Lighting Treatments • Multi task Antiageing Treatments HAIR APPLICATIONS • Sun&Color Protection Complex • Antiageing Hair Treatments • Metal Neutralizer
Toxicological Certificate of human health safety assessment of cosmetic raw materials. OLIVE ACTIVE HIDROXITIROSOL 40% Inci name: Olea europaea (olive) fruit extract, Hydroxytyrosol. Recommended dosage: 0.2% OLIVE ACTIVE HIDROXITIROSOL LPD Inci name: Water, Glycerin, Sorbitol, Lecithin, Olea europaea (olive) fruit extract, Hydroxytyrosol, Alchohol Denat, Xantham gum, Phenoxyethanol, Caprylyl Glycol, Disodium EDTA, Glyceryl Caprylate.
Recommended dosage: 2% OLIVE ACTIVE HIDROXITIROSOL HMA Inci name: Water (Aqua), Cocotrimonium Methosulfate, Olea europaea (olive) fruit extract, Hydroxytyrosol, Alcohol denat., Behentrimonium chloride, Polyquaternium-68, PPG-15 Stearyl ether, Hydroxypropy guar, Phenoxyethanol, Caprylyl Glycol, Glycerin, Glyceryl Caprylate, Phenylpropanol, BHT.
Recommended dosage: 2% - 5%
EG ACTIVE COSMETICS S.L.
info@egactivecosmetics.com www.egactivecosmetics.com
Avda. Diagonal 539, 4º1º 08029 Barcelona (Spain) Tel: + 34 93 209 32 32 Fax: +34 93 667 84 34