CBNS Annual Report 2018 by SCDesign

Annual Report 2018

Collaborating Organisations

Partner Organisations

Feature photography of CBNS staff, students and laboratories throughout this report by Joe Vittorio. Other photographs and images courtesy of CBNS members and organisations, unless otherwise attributed. Content compiled and edited by Samantha Snowden-Finch and Anne Meyer. Graphic design by Sophie Campbell, SCDesign.

ab o ut CBNS Directorâ&#x20AC;&#x2122;s Report

CBNS at a Glance

Strategic Plan

Research

Research Overview

Signature Projects

Overarching Projects

Cross-Centre Activities

Strategic Projects

Engagement 37 Collaborations and Partnerships

CBNS Experience

Events

Education Committee

Mentoring Program

Outreach Sub-Committee

CBNS Awards

Media Coverage

Publication Highlights

Governance

Governance and Management

Governance Board

Scientific Advisory Board

Performance

Performance and KPIs

Financial Report 2018

e n gag e m e nt

g ove r nan c e

About CBNS

R e s ear c h

Contents

CBNS Personnel

Visitors to CBNS

Publications 78

CBNS Annual Report 2018 1

p e r fo r man c e

Awards, Memberships and Grant Success 67

About CBNS The Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology or, as we prefer to be known, ARC Centre for Bio-Nano Science (CBNS) was established in mid-2014 as a national innovator in bio-nano sciences. We bring together a diverse team of Australiaâ&#x20AC;&#x2122;s leading scientists with the aim of developing next-generation bio-responsive nano-structured materials. The key scientific aim that underpins all the activities of the Centre is to fully understand and then exploit, the interface between nano-engineered materials and biological systems. The CBNS research program is structured around the applications of understanding this interface: drug and gene delivery; vaccines; bio-imaging â&#x20AC;&#x201C; both cellular and whole body imaging; and sensors and diagnostics. CBNS research is integrated by overarching research activities to understand the social dimensions of bio-nanotechnology, to visualise bio-nano interactions, and by using a systems biology approach to fully

Health and biomedical sector engagement

describe the complex interactions that dictate success or failure of nanotechnology for therapeutic applications. In delivering the promise of bio-nano science, CBNS has brought together Australiaâ&#x20AC;&#x2122;s leading scientists and engineers with expertise in nanotechnology, polymer science, cell biology, cancer biology, systems biology, chemical engineering, 3D CGI, immunology, chemistry and social science. The Centre consists of five collaborating organisations (nodes): Monash University (Monash), the University of Melbourne (Melbourne), the University of Queensland (UQ),

Centre for Bio-Nano Science

the University of New South Wales (UNSW) and the University of South Australia (UniSA). In addition, there are nine overseas partners and the Australian Nuclear Science and Technology Organisation. CBNS is a seven-year program of research and is funded by the ARC ($26M) and the Australian collaborating universities ($9M) over this period. The universities also contribute in-kind, as do the partner organisations. In total the in-kind support for CBNS is valued at more than $23M over the life of the program and we thank all of the contributors for this support.

Education and training

Delivery (delivery systems, vaccines)

Computational biology

Understanding and exploiting the bio-nano interface

Social dimensions

Detection (sensors and diagnostics, imaging technologies)

Community outreach

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Visualisation

Industry and commercial engagement

ab o ut CBNS CBNS Annual Report 2018 3

Director’s Report Welcome to the fifth Annual Report of the ARC Centre for Bio-Nano Science (CBNS). This report covers our activities in 2018. CBNS can look back at sustainable growth and outstanding achievements in 2018 and will continue its success in 2019. The Centre has built on producing high-calibre research outputs through its refined research direction and an improved strategic plan. We continue engaging with our communities through the successful delivery of events, outreach activities, industry collaborations and interaction with the media. We are proud that our members have been acknowledged through an array of awards and honours during this year. Highlights are captured in this welcome message, while a comprehensive listing is provided within the pages of this report.

Research Themes and Strategic Plan In 2018, we revised the structure of our research. It now includes three research themes that underpin the research program: Delivery Systems and Vaccines which is led by Professor Chris Porter (Monash) and Professor Stephen Kent (Melbourne), Imaging and Visualisation, led by Professor Andrew Whittaker (UQ) as well as Sensors and Diagnostics, led by Professor Justin Gooding (UNSW). The overall research goal for each theme will be achieved through key signature projects. In addition, four overarching research projects have been developed which span the three research themes. Together, these projects illustrate the coordinated approach and complementing research conducted across CBNS. Additionally, it will ensure that fundamental discoveries in bio-nano science and technology enable a range of end-user applications. We also reviewed our Strategic Plan (see pages 8-13) through a broad consultation process with members of CBNS and stakeholders. It provides

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a framework from which CBNS can deliver world class, innovative research. With this new Strategic Plan, the Centre will leave a legacy of research excellence and innovation, together with a highly skilled and professional workforce, ready to lead bio-nano science research into the future.

Growth CBNS has built on its collaborative character and is a truly international centre. The Centre brings together 175 higher degree research candidates and 181 researchers from 32 countries. These researchers make a substantial contribution to CBNS as well as broaden our scientific and public profile.

Engagement We can look back at multiple activities and events that CBNS members organised, contributed to and supported which engaged national and international expert and general audiences alike. These included the 9th International Nanomedicine Conference at Coogee, co-organised and chaired by CBNS researchers, the CBNS and CLEX Media Training, a joint media and communication skills workshop co-organised and facilitated by Alvin Stone from the ARC Centre of Excellence for Climate Extremes (CLEX) and Anne Meyer (CBNS) held at UNSW. In November, the Centre co-organised the ARC Joint Symposium – Advanced Bioengineered Systems, together with three ARC Centres, namely the ARC Industrial Transformation Training Centre for Biopharmaceutical Innovation (CBI), ARC Industrial Transformation Training Centre for Innovation in Biomedical Imaging Technology (CIBIT) and the ARC Industrial Transformation Training Centre in Additive Biomanufacturing (ABM).

Our Education Committee continued its work in creating and delivering programs and workshops that enhance the capabilities and skills of CBNS members. These activities included scientific skills training events such as the Strategic Academic workshop and the Illustrator workshop, participating in the Open House Melbourne event together with the Outreach Sub-Committee as well as co-organising a digital imaging workshop for Fiji/Image J software. The Outreach Sub-Committee built on its inaugural work of 2017 and organised a number of events and activities that engaged the public. Those programs fulfil the important task of increasing public awareness of the research being undertaken at the Centre. This involved three highly successful National Science Week activities including the 3rd Science in the Cinema event screening of the 1966 sci-fi classic Fantastic Voyage (held in Melbourne), participating in the Brisbane Science Festival and organising an exhibition together with the Museum of Discovery in Adelaide. In October, CBNS members carried out a unique roadshow visiting local secondary schools at each node city. Members from all levels promoted the Centre’s research and answered students’ questions about a career in science. More about this special activity can be found on page 50 in this report. As part of the CBNS Visiting Professor Program, we welcomed Professor Warren Chan from the University of Toronto, Canada to the Centre in June. During his stay in Australia, Professor Chan gave a plenary talk at the 9th International Nanomedicine Conference and visited all of the Centre’s node cities. More details of his visit will be covered on page 45 of this report.

ab o ut CBNS A number of significant international exchanges were also instigated to our international partners including the one-month lab visit of CBNS PhD students Ava Faridi and Marvin Wang (both Monash) to Tongji University in Shanghai, China and CBNS PhD students Hayley Schultz and Chelsea Thorn (both UniSA) visiting labs and pharmaceutical companies in the USA and Canada.

Funding In February, CBNS CIs Professor Justin Gooding and Professor Maria Kavallaris (both UNSW) were awarded an ARC Linkage Project grant for their project Bioinks for the 3D printing of cells made from off-the-shelf components worth $416,287. This project was a collaboration with Scientific Advisory Board member Dr Julio Ribeiro from Inventia Life Science Pty Ltd. The project aims to develop a simple method for creating complex, multiple-cell-type three-dimensional (3D) cell cultures for in vitro cell-based assays. We are also proud to report that CBNS CI and Node Leader Professor Benjamin Thierry (UniSA) has been awarded a NHMRC Project Grant worth $432,192. It aims to demonstrate the feasibility of detecting preoperatively the presence of metastatic deposits in regional lymph nodes in head and neck cancer using immuno-PET imaging. If successfully translated to the clinic, this novel imaging-assisted cancer surgery

modality would have major benefits for patients, sparing approximately 2500 unnecessary neck dissections in Australia per year as well as improving staging accuracy and in turn treatment selection. More details can be found on page 67 in the awards section.

Awards The year saw Centre members continuing to be recognised for their contribution to science. Deputy Director Professor Frank Caruso (Melbourne) was elected a Fellow of the Royal Society of London in May. The Fellowship of the Royal Society are the most eminent scientists, engineers and technologists from, or living and working, in the UK and the Commonwealth. Each year up to 52 Fellows and up to 10 Foreign Members are elected from a group of about 700 candidates. In November, Node Leader Professor Justin Gooding (UNSW) was elected a Fellow of the Australian Academy of Technology and Engineering (ATSE). The academy selected Professor Gooding for his more than 20 years of pioneering and sustained contributions to the field of biosensor research. “As an outstanding innovator, research leader and mentor, the level of respect he enjoys in his field and across the sector is clear from his string of senior appointments, influential roles and

awards, as well as his election to the fellowship of the Australian Academy of Science”, the academy wrote on their website. This year’s success is also reflected in the recognition of three CBNS CIs and four CBNS PIs in the Highly Cited Researchers 2018 list. This list recognises world-class researchers selected for their exceptional research performance, demonstrated by the production of multiple highly cited papers that rank in the top 1% by citations for field and year in Web of Science. We are proud that the Centre’s Deputy Director Professor Frank Caruso (Melbourne), CI Professor Chris Porter (Monash) and myself are recognised together with our Partner Investigators Professor Dave Haddleton (University of Warwick), Professor Kenneth A. Dawson (University College Dublin), Professor Craig Hawker (University of California, Santa Barbara) and Professor Molly Stevens (Imperial College London). More awards have been documented on page 67. We look forward to more successful research, engagement and collaboration in the upcoming two years.

Professor Tom Davis Centre Director

CBNS Annual Report 2018 5

CBNS at a Glance Timeline 54 months into 84 month program 2014 2015 2016 2017 2018 2019 2020 2021

Funding $9M in University funding

Partners and collaborators

$35M

over 7 years

$26M in ARC funding

Australian universities

partner organisations

Key data

28 publications in journals with impact factors >10

Our people

Chief Investigators

talks given

new fellowships awarded

18 Senior research fellows undertaking CBNS research

Post-doctoral researchers

371 people

114

Honours and Masters students

5 Management, administration and operational staff

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Diverse disciplines: bioimaging, cell biology, chemistry, engineering, immunology, pharmacology, sociology, systems biology, visual arts

Partner Investigators

175

Research assistants and technical staff

PhD students

US Partner Organisations: Columbia University Medical Center Memorial Sloan Kettering Cancer Center (New York) University of California, Santa Barbara University of Wisconsin-Madison

European Partner Organisations: University of Nottingham University of Warwick Imperial College London University College Dublin

Asian Partner Organisation: Sungkyunkwan University, Korea

Australian Partner Organisation: ANSTO (Sydney)

Collaborating Organisations

University of Queensland

University of New South Wales University of South Australia Monash University (Administering Organisation) University of Melbourne

CBNS Annual Report 2018 7

ab o ut CBNS

Partner Organisations

Strategic Plan We reviewed our Strategic Plan to provide a framework from which CBNS can deliver world class, innovative research in bio-nano science. This work was done through a broad consultation process with members of CBNS and stakeholders. Through this new Strategic Plan, the Centre will leave a legacy of research excellence and innovation, together with a highly skilled and professional workforce, ready to lead bio-nano science research into the future. A summary of the Strategic Plan is provided on pages 8-13.

Our Vision

Our Values

CBNS envisions a world where new technologies are used to understand, treat and diagnose conditions with nanoscale precision.

Excellence CBNS is a global leader in bio-nano science discovery research and innovation, delivering excellence across the scientific endeavour.

Our Mission CBNS will conduct cutting edge research and discovery science to understand the interface between nano-engineered materials and biological systems and translate these discoveries into better methods, tools and technologies.

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Belonging CBNS welcomes and embraces all members, perspectives, ideas and experiences; fostering an environment with equal opportunity for all. Nurture CBNS is a place where new ideas are nourished and members are inspired, motivated and empowered to move towards their personal career goals.

Collaboration CBNS promotes team approaches that capitalise on the multidisciplinary expertise of its members to innovate, imagine and lead research in bio-nano science and technology. Integrity Responsible, respectful, honest, ethical and professional conduct underlies all CBNS research activities, leadership and operations.

ab o ut CBNS

Our Priorities 1. Conduct world-class transformative research that is innovative, collaborative and informed by the multi-disciplinary expertise of CBNS members and their strong national and international networks 2. Inspire, train and nurture the next generation of Australian leaders in science and technology 3. Strengthen connections with stakeholders, industry and end-users 4. Connect CBNS to the public 5. Foster a diverse, equal-opportunity and family-friendly workplace

CBNS Annual Report 2018 9

Organisational Structure â&#x20AC;&#x201C; effective from 2019 A revision of the governance and organisational structure of CBNS has led to the addition of an Industry Partners Program. This change was made to ensure appropriate oversight was provided to meet the strategic priorities focused on increasing industry and stakeholder engagement. All other governance and organisational structures remain in place.

Executive Committee

Scientific Advisory Board

Governance Board

Director: Professor Tom Davis Deputy Director: Professor Frank Caruso

Chair: Professor Alan Rowan Centre Manager Dr Natalie Jones

Chair Education and Training Program

Chair: Professor Peter Doherty Chair Industry Partners Program

Chair Outreach Program

Centre Administration

Education and Training Program Chair Professor Maria Kavallaris

Monash node

Melbourne node

Node Leader Professor Tom Davis

Node Leader Professor Frank Caruso

Outreach Program Chair Associate Professor Matthew Kearnes

UQ node

UNSW node

UniSA node

Node Leader Professor Andrew Whittaker

Node Leader Professor Justin Gooding

Node Leader Professor Benjamin Thierry

Industry Partners Program Chair Associate Professor Kris Thurecht

Research Program Research Themes Delivery Systems and Vaccines

Sensors and Diagnostics

Imaging and Visualisation

Signature Projects Chief Investigators, Co-lead Investigators, Post-doctoral researchers, Research staff, Research students

10 CBNS Annual Report 2018

Strategic Priorities and Goals PRIORITIES

GOALS

Conduct world-class transformative research that is innovative, collaborative and informed by the multi-disciplinary expertise of CBNS members and their strong national and international networks.

To better predict, control and visualise the myriad interactions that occur between nanomaterials and the biological environment and translate these research discoveries into better methods, tools and technologies.

Inspire, train and nurture the next generation of Australian leaders in science and technology

To be a place where research leaders are created and armed with the skills and network they need to meet future challenges.

Strengthen connections with stakeholders, industry and end-users

Connect CBNS to the public

To be a conduit for information transfer between the scientific community and the public, increasing awareness, understanding and acceptance of bio-nano science and technology.

Foster a diverse, equalopportunity and familyfriendly workplace

To be an environment that reflects and embraces diversity, provides equal opportunity for all and is family-friendly, so all members feel welcome, supported and safe.

To facilitate new opportunities for CBNS collaboration and create processes for increased sharing of knowledge, expertise and ideas across CBNS.

CBNS Annual Report 2018 11

Goals and Initiatives

To better predict, control and visualise the myriad interactions that occur between nanomaterials and the biological environment, and translate these research discoveries into better methods, tools and technologies • Establish bio-nano standards for international use • Develop new imaging technologies, assays, tools and capabilities to understand the nano-engineered material and biological interface, including 3D bioprinting, multicellular organoids/spheroid models, lab-on-chip and microfluidics • Use virtual reality technologies to explore and enhance society’s understanding of new bio-nanoscale technologies

To be a place where research leaders are created and armed with the skills and network they need to meet future challenges. • Develop a centrally coordinated career pathways and professional development program for CBNS members, that recognises the diverse career paths open to CBNS staff and students • Develop PhD and EMCR networks at each node, and a PhD and EMCR CBNS committee, comprised of the leaders of each network from each node • Continue to support the Education and Training program to provide members with ongoing scientific skills and professional training • Improve and coordinate communication across CBNS to ensure all members have timely and equitable access to information, opportunities and resources regardless of their geographical location • Establish pathways for succession planning

• Promote collaboration and utilise the multi-disciplinary expertise that exists across CBNS • Encourage discovery, by supporting and testing new ideas and new ways of thinking about bio-nano science and technology

To facilitate new opportunities for CBNS collaboration and create processes for increased sharing of knowledge, expertise and ideas across CBNS. • Create time for both professional and social networking at CBNS events • Promote the use of videoconferencing as a platform for regular exchange of knowledge, ideas and information between members located at different sites and nodes • Increase awareness of CBNS exchange programs that support placement of CBNS members to work in laboratories of other CBNS members

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To increase engagement with stakeholders, industry partners and other relevant end-users, to support ongoing research activities, promote translation of research findings and ensure the research directions of CBNS respond to the changing social and political environment. • Build the profile of CBNS to ensure its reputation as a national focal point of research excellence in bio-nano science is recognised by other scientists, industry and end-users. This will create new opportunities for continued scientific endeavours, including implementation and uptake of research outcomes • Develop an Industry Partners Program, to coordinate industry engagement activities, build networks, expand mentoring program and support ongoing relationships and communication with industry and end-users. This program will: – Develop formal partnership events, to promote CBNS research to industry and end-users – Create a translation strategy, with advice from broader industry and end-user groups, including health services and agricultural scientists, to create a pipeline from knowledge creation to implementation and impact

To be a conduit for information transfer between the scientific community and the public, increasing awareness, understanding and acceptance of bio-nano science and technology. • Continue to support and build on the current Outreach Program to communicate information about bio-nano science and technology to the public, through primary and secondary schools, public events, national science week and other university and CBNS initiatives • Evaluate and increase social readiness for bio-nano science and technology innovations, to ensure research outcomes are attractive, understood and suitable for the public • Build the reputation of CBNS as the focal point of bio-nano science knowledge and expertise in Australia and facilitate bi-directional communication between the scientific community and the public

To be an environment that reflects and embraces diversity, provides equal opportunity for all and is family-friendly, so all members feel welcome, supported and safe. • Raise awareness and regularly review the diversity, gender equality and family-friendly practices within CBNS policy, to ensure it supports the creation of an inclusive, equitable and family friendly culture • Promote opportunities for career development for all members, to demonstrate our support and commitment to inclusion across sex, race, gender, age, religion, identity and experience. This includes participation in the mentoring program,and on internal and external committees, providing opportunities to present at conferences, workshops and forums, representing CBNS at scientific societies, participating in advocacy roles and in the media and public events • Seek ongoing feedback and suggestions to increase support for those with families and care responsibilities to attend internal CBNS and external events

Research Goals

Delivery Systems and Vaccines • To facilitate a priori design of nanomaterial-based delivery systems that can 1) target specific organ and cell types and 2) deliver therapeutically active cargoes to precise cellular and intracellular destinations • To understand interactions of nanomaterials with the immune system to enable nanoengineered materials to improve vaccine design and efficacy

Sensors and Diagnostics

Imaging and Visualisation

• To be able to detect and quantify, with minimal sample preparation and without calibration or amplification, rare biomolecular entities directly in biological fluids

• To lead the field of the science of imaging and visualisation by addressing the three main elements of the imaging “pipeline”: 1) image acquisition, 2) simulation and modelling and 3) visualisation

• To be able to detect, characterise and manipulate rare mammalian and bacterial cells in whole blood • To integrate these advanced separation and detection abilities within platform technologies towards the development of the next generation of precision sensors

• To provide clear teaching of all aspects of synthesis of high-performing and responsive imaging agents, image acquisition and interpretation of results (including development of physics-based models) and visualisation

CBNS Annual Report 2018 13

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R e s ear c h

Research CBNS Annual Report 2018 15

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Research Overview In line with the updated Strategic Plan, CBNS has revised the research structure to focus on four elements: the engineering of nanomaterials; the study of the interaction between these materials and the biological systems; the stimulation on immune responses; as well as the reporting of biological events. The revised structure includes three research themes that underpin the research program:

2 Imaging and Visualisation – led by Professor Andrew Whittaker (UQ) 3 Sensors and Diagnostics – led by Professor Justin Gooding (UNSW) Each theme has one or more signature project/s that will work towards achieving the research goals. Additionally, four overarching projects have been developed which span the research themes. Together, these projects illustrate the coordinated approach and complementary research conducted across the Centre and will ensure that fundamental discoveries in bio-nano science and technology enable a range of end-user applications. The signature projects capture the ultimate application of collaboration within the Centre’s research: every CBNS researcher engages in at least one of these signature projects embracing the collaborative spirit of the Centre.

Overarching projects Social dimensions of bio-nano interactions (page 24)

Predicting bio-nano interactions (page 25)

Development of complex cellular systems for the evaluation and characterisation of bio-nano interactions (page 26)

Improved molecular imaging agents (page 27)

Research themes:

Delivery Systems & Vaccines

Sensors & Diagnostics

Imaging & Visualisation

Signature projects:

A material scientists guide to the cell (page 18)

New tools for quantifying and manipulating rare entities directly in biological fluids (page 21)

JTCC: Visualising bio-imaging and data in 3D virtual reality (page 22)

Overcoming challenges of in-vivo targeting (page 19) Mediating protein interactions (page 20)

The research program and expertise within CBNS is presented in more detail in the following pages.

CBNS Annual Report 2018 17

R e s ear c h

1 Delivery Systems and Vaccines – led by Professor Chris Porter (Monash) and Professor Stephen Kent (Melbourne)

Signature Projects Delivery Systems & Vaccines A material scientist’s guide to the cell Leaders: Dr Angus Johnston (Monash), Professor Rob Parton (UQ) Co-Leaders: Dr Nik Veldhuis (Monash), Dr Laura Selby (Monash) Collaborating organisations/groups: Professor Cameron Alexander (University of Nottingham), Professor Jason Lewis (Memorial Sloan Kettering Cancer Center), Associate Professor Steven Faux (St Vincent’s Clinic), Professor Maria Kavallaris (UNSW), Associate Professor Kris Thurecht (UQ)

The project Effective drug delivery depends on delivering drugs to the sites in the cell where they are therapeutically active. Nanoparticles are an attractive strategy for delivering agents in a controlled and precise manner. The aim of this project is to gain a fundamental understanding of how nanoparticles are trafficked in cells. In particular, we are focusing on understanding how nanoparticles and their cargo are internalised, trafficked in the endosomes and then move from the endosome into the cytoplasm (i.e. endosomal escape). These fundamental interactions are poorly understood and by understanding these interactions, we aim to engineer the next generation of nanoparticles that can respond intelligently to the cellular environment.

Value This work is crucial for achieving nanoparticle delivery, with universal applications in nanomedicine.

Outcome/s

Nanoparticles delivering their cargo into lysosomes.

Understanding how endosomal escape occurs and optimising endosomal escape to improve the efficacy of nanomedicines.

Activities undertaken in 2018 • Developed endosomal escape assays • Tested penetrating peptides

Activities planned for 2019 Endosomal escape in cells: • Testing penetrating peptides as endosomal escape agents • Critical analysis of the proton sponge effect

Electron microscopy image of endosomes.

18 CBNS Annual Report 2018

Overcoming challenges of in vivo targeting Leaders: Associate Professor Kris Thurecht (UQ), Professor Frank Caruso (Melbourne) Co-Leaders: Dr Zach Houston (UQ), Dr Matt Faria (Melbourne) Collaborating organisations/groups: Professor Cameron Alexander (University of Nottingham), Professor Maria Kavallaris (UNSW), Professor Stephen Kent and Dr Georgina Such (Melbourne), Professor Chris Porter and Dr Angus Johnston (Monash), Professor Steve Mahler (UQ), Dr Brad Walsh (Minomic International)

The project

R e s ear c h

To develop an in-depth understanding of the role that the physicochemical properties of nanomaterials have on overcoming the different biological barriers that can impede nanoparticle-accumulation in tissue.

Value • This work has fundamental scientific importance for understanding cell biology in vivo • Development of the zebrafish as a system to study endocytosis will usher in a new phase of in vivo cell biology • A universal method for studying nanoparticles in a simple in vivo system will be of great interest to scientists and will have commercial benefits; optimising targeted nanomaterials to provide fundamental insight into improved formulations in future therapeutics

Outcome/s • An increased understanding of how a protein or nanoparticle passes from the bloodstream into tissues and how it is taken up by target cells • Study and optimisation of endosomal escape in whole animals

Activities undertaken in 2018 • Development of zebrafish as an in vivo model for nanoparticle delivery • Unravelled the factors that contribute to the enhanced permeability and retention (EPR) effect to optimise targeted accumulation in tumour models

Schematic representation of the different biological barriers encountered by nanomaterials following administration into the bloodstream.

Activities planned for 2019 • Quantitative analysis of nanoparticle trafficking in vivo; mechanisms of and imaging, the transport of nanoparticles across the endothelium; enhancing selective association and uptake into cells in vivo; investigations into mechanisms of endosomal escape • Finding answers to howow does targeting affect biodistribution? What is the optimal targeting density to effect both tissue accumulation and target cell association? Schematic representation of the different biological barriers encountered by nanomaterials following administration into the bloodstream.

Biodistribution of different nanoparticles in mice determined by PET-CT showing the effect of nanomaterial properties on organ accumulation.

CBNS Annual Report 2018 19

Mediating protein interactions Leaders: Dr Pu-Chun Ke (Monash), Professor Frank Caruso (Melbourne), Professor Pall Thordarson (UNSW) Co-Leaders: Dr Yi (David) Ju (Melbourne), Ms Emily Pilkington (Monash), Dr Adam Martin (UNSW) Collaborating organisations/groups: Professor David Haddleton and Professor Greg Challis (University of Warwick), Professor Kenneth Dawson (University College Dublin), Professor Morgan Alexander (University of Nottingham), Professor Raffaelle Mezzenga (ETH Zurich), Professor Feng Ding (Clemson University), Dr Darren Creek (Monash), Dr Paul Wilson (UQ), Professor Sijie Lin (Tongji University), Professor Maria Kavallaris, Professor Martina Stenzel and Professor Lars Ittner (UNSW), Professor Stephen Kent and Professor Frances Separovic (Melbourne), Dr Esteban Gurzov (Université Libre de Bruxelles), Professor Killugudi Swaminatha Iyer (University of Western Australia), Professor Fiona Wood (Royal Perth Hospital Burns Unit)

The project The project aims to develop strategies for controlling protein and peptide structure and aggregation and to understand which biological signals are important to sustain and promote cell growth in biomaterials. Central to these aims is to better understand the mechanism that underpins protein and peptide self-assembly, folding, and misfolding. The knowledge obtained from this work will be essential for developing more effective strategies against amyloid diseases and protein fouling.

Value This work will enable cancer-targeting peptides to be used in the treatment of hard-to-cure children’s brain cancers such as medulloblastoma. The peptide-polymer system could be applied in a more generic manner for drug delivery. This research will shed light on the molecular mechanisms of amyloid diseases and offer nanomedicinal solutions to target this global epidemic.

Outcome/s • Clinical potential of cancer-cell targeting peptide systems established • The formation of a novel zebrafish model will enable high throughput studies of type 2 Diabetes and Alzheimer’s, which are crucially needed, as the current mouse models are expensive and deficient. Domestic (Florey Institute) and international (China, Belgium, US, Switzerland) collaborations have been established to ensure the success of these efforts

Activities undertaken in 2018 • Developed cancer-cell targeting peptides to deliver drugs and nanoparticles with particular focus on using non-spherical polymersomes for delivery

The thermodynamic cycle for subunit folding leading to the formation of the folded abmonomers starting from the unfolded α- and β-subunits (αU, βU).

• Developed a zebrafish model and a protein corona paradigm for delineating and mitigating amyloid diseases

Activities planned for 2019 • Determine blood plasma interactions and bio-distribution of non-spherical polymersome/peptide interactions • Develop a peptide-RNA delivery vehicle • Alzheimers- and Parkinsons-like pathogenesis in zebrafish and mouse models and their mitigation with gold nanoparticles and Cerebral Dopamine Neurotrophic Factor (CDNF) delivery • Real-time amyloid protein aggregation

20 CBNS Annual Report 2018

Particle properties and the biological environment influence particle targeting in complex biological media.

Peptide aggregation and stabilisation in human pancreatic islets.

Sensors & Diagnostics New tools for quantifying and manipulating rare entities directly into biological fluids Leaders: Professor Justin Gooding (UNSW), Professor Benjamin Thierry (UniSA), Dr Simon Corrie (Monash) Collaborating organisations/groups: Professor Stephen Mahler and Associate Professor Kris Thurecht (UQ), Professor Pall Thordarson (UNSW), Associate Professor Therese Becker (Ingham Institute for Applied Medical Research), Professor Katharina Gaus (EMBL Australia Node in Single Molecule Science), Professor Maria Kavallaris (UNSW), Dr Dan Inglis (Bionomics Ltd), Professor Wayne Tilley and Dr Luke Selth (University of Adelaide), Professor Tim Price and Dr Jenny Hardingham (Adelaide Oncology and Haematology, The Queen Elizabeth Hospital)

The project

Value

The unmet need in the biosensing field is to develop technologies that can detect, quantify and even manipulate rare biomarkers. This is important for early disease diagnosis, infection detection and the monitoring and handling of rare cells. Currently, technologies can detect species down to the nanomolar range whereas the amount of biomarkers for the early diagnosis of disease is typically in the picomolar range or lower. In the case of rare cells, the requirements may be to detect and manipulate one in a billion cells. The challenges with such ultrasensitive sensors are having more sensitive transducers, mass transport of the biomarkers to the sensor in a reasonable time frame and achieving good selectivity. This project seeks solutions to these three main challenges that will allow the monitoring of rare biomarkers directly in bodily fluids with minimal sample preparation. The focus will be on rare protein and nucleic acid biomarkers and the manipulation and characterisation of rare mammalian and bacterial cells. The solutions will be integrated into platform technologies that will comprise the next generation of precision sensors.

End-user driven development of liquid biopsy technologies will address crucial health needs and also foster the further growth of the Australian medical diagnostic device industry.

Outcome/s â&#x20AC;˘ The detection and quantification of rare biomolecular entities directly in biological fluids, with minimal sample preparation and without calibration or amplification â&#x20AC;˘ Further detection, characterisation and manipulation of rare mammalian and bacterial cells in whole blood â&#x20AC;˘ Integration of these advanced separation and detection advances within platform technologies towards the development of the next generation of precision sensors

Activities undertaken in 2018 Developed electrochemical and optical techniques for isolating or separating of rare cells and biomarkers from blood, including: fetal cells, bacteria and uRNA.

Activities planned for 2019

Below: 3D illustration showing a biofilm formed in vivo containing antibiotic-resistant bacteria. Once microbial biofilms are established, they are significantly less susceptible to antibiotics. Furthermore, most BSIs originate from biofilms formed on medical devices or anatomical sites.

Development of assays for ultra-sensitive (e.g. atto-molar) detection of rare events following separation, including: blood-tube assays for bacterial cell detection, microfluidic systems solid-state electrochemical and/or nanopore based systems.

CBNS Annual Report 2018 21

R e s ear c h

Co-Leaders: Dr Marnie Winter (UniSA), Dr Ying Yang (UNSW)

Imaging & Visualisation JTCC: Visualising bio-imaging and data in 3D virtual reality Leaders: Associate Professor John McGhee (UNSW), Professor Tom Davis (Monash) Co-Leaders: Dr Andrew Lilja, Mr John Bailey, Mr Campbell Strong (UNSW) Collaborating organisations/groups: Professor Cameron Alexander (University of Nottingham), Professor Jason Lewis (Memorial Sloan Kettering Cancer Center), Associate Professor Steven Faux (St Vincent’s Clinic), Professor Maria Kavallaris (UNSW), Professor Rob Parton and Associate Professor Kris Thurecht (UQ), Dr Angus Johnston (Monash)

The project This project will explore how design-led 3D virtual reality (VR) immersive techniques can be used to visualise the dynamic process within a cancer cell. The research also intends to conduct user testing of the VR content with students and scientific communities. The goal of this research is to establish whether VR immersive data interaction provides benefits as an educational tool. We also intend to investigate whether 3D VR visualisations of laboratory data can facilitate and enhance the scientific discovery process. As part of this evaluation, we will be developing a VR platform to ‘share’ content. The goal is to connect VR 3D cellular data between multiple users and sites across the CBNS nodes simultaneously on a collaborative VR platform, to be termed the ‘VR Cell Arena System’.

Sub-projects • 3D Visualisation of Cancer Cell Data – UNSW, UQ and Monash • 3D VR of High Resolution 3D Cell Dynamics Data – UNSW and CCIA

new type of aesthetic for the public to view and experience nanoparticle delivery in VR. This will lead to a better understanding of alternative modes to visualise nanostructures in both education and science discovery settings.

Outcome/s • NanoScape – Peer review high impact Non-Traditional Research Outputs (NTRO) – VR Film and interactive environment • VR interactive platform for importing and realising visualisation of nanoparticles in vivo • A knowledge base on interactive VR uses in education and public engagement and nanoparticles in vivo

Activities undertaken in 2018 • Prototyped multi-user VR system • Interactive VR mouse visual prototype for nanoparticle viewing and sampling • Prototype Voxel based real-time visualisation tool for VR

• 3D VR in vivo Nanoparticles Dynamics Data – UNSW and UQ

Activities planned for 2019

Value

• Education Vis/Representation nanostructures development and evaluation

The development of effective VR tools for communication in education, about nanoparticle drug delivery, will allow a

• NanoScape 3D Film/360 Video

• Interaction Evaluation – Mouse VR and Multiuser system • Development of VR work with EMBL

Immersive visualisation of the internal structures of a cancer cell. Image: John McGhee, John Bailey, 3D Visualisation Aesthetics Lab, UNSW (@3DVAL).

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R e s ear c h Visualisation of a nanoparticle binding to receptors on the surface of a cancer cell. Image: John McGhee, Andrew Lilja, John Bailey, 3D Visualisation Aesthetics Lab, UNSW (@3DVAL).

Visualisation of vesicle transport within a cancer cell. Image: John McGhee, John Bailey, Andrew Lilja, 3D Visualisation Aesthetics Lab, UNSW (@3DVAL).

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Overarching Projects Social dimensions of bio-nano interactions Leader: Associate Professor Matthew Kearnes (UNSW) Co-Leader: Dr Declan Kuch (UNSW) Collaborating organisations/groups: Professor Stephen Kent (Melbourne), Professor Justin Gooding, Associate Professor Orazio Vittorio and Professor Maria Kavallaris (UNSW), Associate Professor Kris Thurecht and Professor Rob Parton (UQ), Dr Angus Johnston (Monash)

The project Bio-nano technologies, together with advances in precision and personalised medicine, are likely to profoundly change healthcare practices. By exploring the social dimensions of research across this area of work, in collaboration with key CBNS research initiatives, this program will provide insights into the societal dimensions of predictive bio-nano technologies. This project seeks to address key questions related to the intersection between big data, healthcare, personalised and precision medicines and regulation. The proposed program of work will entail the use of the following social science methodologies and will be facilitated by a range of cross-node collaborations: • Social media monitoring and analysis tools (such as NodeXL) will enable issue mapping of the institutional and discursive shaping of research agendas in precision and personalised medicine • Ethnographic observation: close analysis of sciencein-practice will serve to document the imagined social worlds that underpin developments in bio-nano technology, focusing especially on CBNS research projects • Interdisciplinary exchange workshops will bring CBNS researchers into conversations with researchers working in social sciences, humanities and law to explore the broader social dimensions of their work Targeted public engagement initiatives will also form part of the workplan of the program, enabling CBNS researchers to address the societal dimensions of their research in appropriately designed and facilitated public forums.

Value Characterising the sociological dimensions of research in bio-nano science and technology has broad importance for the general public and societal understanding and acceptance of bio-nano science applications in the real world.

Outcome/s This work will result in a number of publications and outreach initiatives against the planned activities. These outcomes will result in increased scientific, public and societal awareness and an understanding of bio-nano science and technology.

Dr Declan Kuch with Ms Kristel Tjandra and Dr Robert Utama discussing polymer chemistry at UNSW during the recording of the Radio National Science Friction episode on ‘Luddites’.

Activities undertaken in 2018 • Finalised research for major papers for leading Social Studies of Science journals, including research on the on the Enhanced Permeability and Retention (EPR) effect and the regulatory and research policy architectures of bio-nano science and technology • Contributed to Australian Council of Learned Academies Report on Precision Medicine • Papers in Science, Technology and Human Values, Life Sciences, Society and Policy • Completion of a PhD project that included research on the social dimensions of transdermal delivery systems • Ccommencement of a UNSW Scientia PhD project exploring the societal dimensions of vaccines research

Activities planned for 2019 • Finalise research monograph drawn from Phase 1 of CBNS research • Continue collaborative analysis of challenges in realising bio-nano research • Commence collaborative project with JTTC team – ethnographic study of nanoparticle visualisation • Commence collaborative project via PhD on sociology of vaccines research • Finalise research on governance and regulation on precision bio-science in Asia/Pacific

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Predicting bio-nano interactions Leaders: Professor Stephen Kent, Professor Edmund Crampin (UNSW) Co-Leaders: Dr Mattias Björnmalm (Imperial College London), Dr Adam Wheatley, Dr Matt Faria (Melbourne) Collaborating organisations/groups: Professor Molly Stevens (Imperial College London), Professor Rob Parton and Associate Professor Kris Thurecht (UQ), Dr Angus Johnston and Dr Simon Corrie (Monash), Professor Frank Caruso (Melbourne)

The project

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Understanding how materials and cells interact will be key to the future development of improved nanomedicines and vaccines. We aim to understand the rules by which immune cells interact with a range of nanoengineered particle systems with tailored physical properties. The combined effects of size, charge, surface chemistry and other physicochemical characteristics will be studied for their effect on particle interactions with a wide range of immune cells.

Value Understanding how materials and cells interact will be key to future development of improved nanomedicines and vaccines. • The experimental and theoretical techniques developed will provide a roadmap for biological analysis of newly developed nanomaterials for the global research community • Our fundamental understanding of the relative importance of nanoparticle physicochemical properties will be advanced, leading to a more rational design of these materials • By increasing our ability to predict bio-nano interactions prior to clinical evaluation, we will vastly accelerate our ability to evaluate and design nanoparticles for diagnostic and therapeutic purposes

Outcome/s This work will increase the understanding of the rules by which immune cells interact with a range of nano-engineered particle systems with tailored physical properties. The research examines the influence and trends of different physicochemical characteristics of nanoparticles, on their interactions with immune cells in vitro and in vivo. This research will demonstrate the potential to standardise the way in which nanoparticle-cell interactions are studied, such that we can generate predictive algorithms for these interactions.

Activities undertaken in 2018 The team developed novel experimental assays and theoretical models that can predict the biological fate of engineered nanomaterials.

Activities planned for 2019 • Use our techniques to assess panels of nanomaterials created by experts across CBNS, advancing our fundamental understanding of how these complex materials interact with biological systems

Single particle simulation of sedimentation and diffusion for two particle systems.

• Compare and contrast particle systems and particle modifications that allow the understanding of general principles of bio-nano interactions • Focus on the role of the protein corona on bio-nano interactions

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Development of complex cellular systems for the evaluation and characteristics of bio-nano interactions Leaders: Professor Maria Kavallaris (UNSW), Professor Benjamin Thierry (UniSA) Co-Leaders: Dr Frieda Mansfeld (UNSW), Dr Fran Ercole (Monash) Collaborating organisations/groups: Professor Jason Lewis (Memorial Sloan Kettering Cancer Center), Professor Cameron Alexander (University of Nottingham), Professor Richard Lock (CCIA)

The project A major challenge in the development and implementation of effective nanomedicine is the lack of preclinical models that recapitulate the complexity of the complex cellular systems and microenvironments. Towards accelerating the development of nanotechnology strategies that target specific organ and cellular systems, we are developing the next generation of in vitro models designed to replicate physiological and biological systems relevant to the characterisation and evaluation of bio-nano interactions. Ultimately, these advanced models will guide the development of nano-based diagnostic and therapeutic strategies better tailored to specific diseases.

Value The development and characterisation of bioengineered microfluidic models will enable accurate prediction of healthy and tumour tissue responses to cancer therapeutics as well as an assessment of novel nano-based nanoparticle interactions for broader applications.

Outcome/s

An “intestinal epithelium on a chip” in vitro model to study the uptake of nanoparticulate carriers (green in the image) and prodrugs in the small intestine: Exposed to microfluidic fluid shear, intestinal cells (nuclei in yellow) undergo rapid differentiation and spontaneously produce a protective layer of mucus (magenta).

Bioengineered microfluidic and 3D bio-printed models will enable the prediction of more physiologically relevant responses of healthy and disease tissue responses and serve as a more relevant pre-clinical model for nano-based therapies for cancer and other diseases

Activities undertaken in 2018 • Developed novel 3D bio-printing technology and methods for the design of high throughput bio-assays • Developed and characterised bioengineered microfluidic vascularised tissue models to enable the accurate prediction of healthy cellular responses to radiation therapeutic modalities • Characterised bioengineered microfluidic intestinal model for assessment of nanoparticle uptake

Activities planned for 2019 • Develop and characterise bioengineered vascularised microfluidic tumour models for assessment of nano-based immune-therapeutics • Implement 3D bioprinting technology to improve fabrication of in vitro cellular and microfluidic models

Childhood brain cancer cell showing star-polymer nanoparticles (green) in the cell cytoplasm. Nucleus is blue and cell membrane is orange. (Image: Helen Forgham)

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Microvascular network formed within vasculogenesis microfluidic devices provides new insights about the effect of radiotherapy on blood vessels (Guo et al., Advanced Technical Materials 2019).

Improved molecular imaging agents Leaders: Professor Andrew Whittaker (UQ), Professor Tom Davis (Monash) Co-Leaders: Dr Jeroen Goos (Memorial Sloan Kettering Center), Dr Ruirui Qiao (Monash), Dr Cheng Zhang, Dr Changkui Fu and Dr Simon Puttick (UQ)

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Collaborating organisations/groups: Dr Ivan Greguric (ANSTO), Professor Jason Lewis (Memorial Sloan Kettering Cancer Center), Professor Thomas Nann (MacDiarmid Institute), Professor Craig Hawker (University of California), Dr Sophie Laurent (Université de Mons), Professor Debra Bernhardt and Professor Maree Smith (UQ), Professor Stephen Rose (CSIRO), Professor Guangjun Nie (National Center for Nanoscience and Technology of China), Professor Afang Zhang (Shanghai University), Professor Zushun Xu and Professor Ling Li (Hubei University), Professor Fabienne Dumoulin (Gebze Technical University), Professor Petr Kral (University of Illinois at Chicago), Professor Simon Swift (University of Auckland), Professor Kishore Bhakoo (A*STAR)

The project The project team aims to improve the resolution and accuracy of molecular imaging at different physiological sites and to exploit nanotechnology for specific diagnosis and theranostic applications.

Value There is currently a re-evaluation of several aspects of nanomedicine, in particular delivery efficiency. Imaging agents will play a crucial role in advancing the field by enabling quantitation and higher-level information in the case of multi-modal or responsive agents.

Outcome/s Imaging is a central technology in the field of nanomedicine. There are a plethora of current approaches and the primary outcome of this overarching project will be a clear direction on the design and choice of imaging agent.

Activities undertaken in 2018 The team have designed, prepared and evaluated, in animal models, three new material classes as MRI or multimodal agents.

PET/CT showing the distribution of a functional star polymer (S. Puttick/M.R. Whittaker), showing uptake in the bone marrow.

Activities planned for 2019 • Study biological interactions: 1. Blood-NP interactions 2. Biological fate 3. Protein corona • Develop an understanding of the relationship between nanoparticle design and cellular interactions. From this, design responsive agents

Superposition of PET/CT images of a mouse with a brain tumour xenograft, after injection with a 64Cu labelled antibody.

Snapshots of MD simulation of bovine serum albumin interacting with oligomeric polymer acrylic acid taken at the end of 20 ns of MD simulation.

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Cross-Centre Activities Bio-responsive nanocarriers for the eradication of bacterial and fungal biofilms CBNS Chief Investigators involved: Professor Clive Prestidge (UniSA), Professor Ben Boyd and Professor Tom Davis (Monash) CBNS Researchers: Dr Mikey Whittaker and Dr John Quinn (Monash), Dr Nicky Thomas (UniSA) CBNS PhD students: Ms Chelsea Thorn and Ms Yassamin Albayaty (UniSA) Biofilm-associated infections are considered a catastrophic healthcare problem. Bacterial and fungal biofilms are the leading cause of infections ranging from simple superficial lesions to severe systemic life-threatening infections. In a biofilm, microorganisms accumulate and produce an extracellular matrix, which provides protection against many antimicrobial agents. This protection is complex and multifunctional, resulting in the failure of all antimicrobials. The efficacy of antibacterial and antifungal drugs in the eradication of biofilms relies not only on their intrinsic antimicrobial activity but also on their bioavailability at the infection site. The scope of this new cross-node research program is to explore infection responsive nanocarriers that can deliver specific anti-biofilm agents directly to the site of action, increasing bioavailability and efficacy.

Antifungal delivery via pH-responsive polymeric micelles Itraconazole is a potent antifungal therapeutic agent, however, its hydrophobic structure and low solubility at physiological pH (< 1µg/ml) negatively impacts efficacy against biofilms. Novel nanocarriers have potential to overcome drug and biofilm-related barriers and treat biofilm infections. Furthermore, pH-responsive nanocarriers can trigger local release and accumulation of the drug within the acidic environment associated with a biofilm and hence improve drug efficacy; this has been demonstrated for antibacterial agents, but not with antifungal agents. Here we have developed itraconazole-loaded micelles from the pH-responsive block copolymer poly[(N,N-diethylamino) ethyl methacrylate-block-polyethylene glycol methacrylate] (P[DEAEMA-b-PEGMA]). It is hypothesised that low pH within the biofilm triggers micelle destabilisation and targeted itraconazole release/delivery. Biofilms containing C. albicans were investigated using an in vitro crystal violet assay for biofilm biomass, a colony forming unit assay and live/dead stain confocal fluorescence images.

Key goals for 2019 1 Quantify pH-controlled release and determine mechanism of action 2

Anti-biofilm efficacy of itraconazole loaded micelles on different fungal strains

Figure 1: pH-sensitive polymeric micelles against fungal biofilms

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Reducing biofilm biomass by enhancing itraconazole penetration • Itraconazole-loaded pH-sensitive micelles resulted in a significant reduction in the biomass of C. albicans biofilms (>50%) in comparison with free drug (< 20%). This demonstrates the potential of pH-sensitive micelles in enhancing efficacy against fungal biofilms. • Confocal images of two-days-old fungal biofilms treated with itraconazole-loaded micelles show significantly higher levels of dead fungal cells in comparison with free itraconazole. This improved efficacy is considered to be due to improved drug solubilisation, biofilm penetration and triggered drug release.

Pseudomonas lipase responsive liquid crystals for glycoside hydrolase and antibiotic combination delivery Triggered release and anti-biofilm activity • The bulk GMO liquid crystal gel was not completely hydrolysed by Pseudomonas lipase, however, when the gel was dispersed into cubosomes, complete hydrolysis was achieved, resulting in a triggered release of AL and gentamicin. • The bulk gel maintained the anti-biofilm activity of AL and gentamicin, however, the GMO liquid crystal nanoparticles resulted in a 25% increase in total anti-biofilm activity of the combination. The enhanced activity is hypothesised to be related to the small size and lipase-driven hydrolysis of the cubosomes.

Key goals for 2019 1 Quantify the safety and stability of the cubosome delivery system with AL and gentamicin

Implement the cubosome delivery system with novel glycoside hydrolases developed by collaborators at the SickKids Hospital in Toronto, Canada

Figure 2: Liquid crystals against bacterial biofilms.

References: 1. Thorn, C. R., Prestidge, C.A., Boyd, B.J., Thomas, N., (2018). Pseudomonas Infection Responsive Liquid Crystals for Glycoside Hydrolase and Antibiotic Combination. ACS Applied Bio Materials. 1 (2), 281–288.

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Glycoside hydrolases are enzymes that disrupt the extracellular matrix surrounding biofilms, thereby dispersing the bacteria and increasing their susceptibility to antimicrobials. However, they are sensitive to proteolysis and aggregation and benefit from controlled delivery. We hypothesise that liquid crystalline nanocarriers based on glyceryl monooleate (GMO) can effectively encapsulate a combination of large glycoside hydrolase and small antibiotic molecules of varying hydrophilicities to overcome the challenges for effective delivery to biofilms and hence maximise stability, bioavailability and efficacy. Furthermore, since bacteria readily produce lipases, there is potential to facilitate triggered release within biofilms, as pictorially demonstrated by the Rubik’s Cube in Figure 2. Initial investigations have focused on GMO cubic phase gel and the dispersed phase cubosomes for biofilm delivery of alginate lyase (AL) and the antibiotic gentamicin.

Interactions of nano-materials and immune cells Immunity and bio-nano science Leader: Professor Stephen Kent (Melbourne) CBNS Chief Investigators involved: Professor Frank Caruso and Professor Edmund Crampin (Melbourne), Professor Tom Davis, Dr Angus Johnston and Dr Simon Corrie (Monash), Professor Andrew Whittaker and Associate Professor Kris Thurecht (UQ), Professor Pall Thordarson (UNSW) Immune cells play critical roles in both the desired actions of nanoparticles (for example by stimulating Dendritic cells, B cells and T cells) and the clearance of nanoparticles (e.g. by neutrophils and macrophages in a wide range of biological systems. The complexity of immune cells present in tissues and fluids presents a major challenge in unravelling important interactions between nanomaterials and biological systems. Further, nanomaterials can be engineered with a range of properties (including, but not limited to, size, shape, charge, flexibility, surface materials, functionalisation with antibodies and the presence of protein coronas derived from host proteins). All these characteristics will influence association, uptake and the biological impact of materials on immune cells. Combinations of nanoparticle characteristics (such as varying size, shape and/or charge simultaneously) are likely to have influence on biological interactions in important ways, but this may be difficult to predict in advance. An enormous strength (likely unparalleled globally) of CBNS is the wide range of particle chemistries and expertise across the Chief Investigators and nodes. This allows for opportunities to directly compare particle designs

and chemistries for their influence on immune cell interactions. CBNS produced several major advances in understanding how one single parameter of particles that vary in one parameter can influence in their association with, and biological impact on, function in across many human immune cell types. We have exploited a model whereby we cultivate nanomaterials with healthy human blood. A major advantage of this model is that it mimics natural conditions and uses primary cells rather than cell lines. In this manner, we have recently demonstrated the influence of novel â&#x20AC;&#x153;stealthy materialsâ&#x20AC;? [1], of protein coronas[1, 2, 3] and of particle immunoreactivity on bio-nano interactions involving human blood cells. We continue to work towards using matrices of nanoparticle characteristics and to translate this work into cellular interactions in immune organs such as lymph nodes in mouse systems. Our careful work characterising bio-nano interactions has allowed us, as a large consortium, to understand and publish the important parameters that need to be considered when studying bio-nano interactions in the laboratory[4].

Use human blood assay to predict in vivo behaviour of nanoparticles In vitro assays using immune cell-derived cell lines in culture media are currently a common tool to predict nanoparticle interactions with the immune system. However, these tests do not fully represent the physiological interactions of nanoparticles with primary cells in the more complex environment of fresh blood and therefore difficult to predict nanoparticle distribution to reticuloendothelial system organs in vivo. The human blood-nanoparticle interaction assay provides a useful basis to fill the gap between the in vitro cell line-based

assay and the in vivo findings. It provides detailed information on nanoparticle uptake by different blood cell types in whole blood. Our work with novel stealthy poly(2-ethyl-2-oxazoline) (PEtOx) based particles has shown minimal interactions with human blood proteins and a high level of biocompatibility[1]. The role of human blood proteins in influencing (and predicting) nano-material interactions with human immune cells is now being studied across a range of particle systems available through CBNS.

Figure 1: Synthesis of PEG and PEtOx and their grafting onto nGO (carbon: brown, oxygen: red, hydrogen: blue).

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Hyperbranched polymers have been studied collaboratively by CBNS over the last 2 years. We have synthesised charge-varied hyperbranched polymers (HBPs) and demonstrated surface charge as a key parameter directing their association with specific human blood cell types. Using fresh human blood, we investigated both the association of HBPs with six white blood cell populations in their natural milieu by flow cytometry and studied their biodistribution in mice. We found that, regardless of charge, HBPs had minimal effects on coagulation, platelet, complement or T cell activation. However, high concentrations of cationic HBPs led to significant dendritic cell activation, suggesting the potential application of these nanoparticles as vaccine adjuvants to

aid efficient antigen presentation. Biodistribution studies showed that intravenously administered charge-neutral HBPs had a longer retention time in the circulation than cationic or anionic HBPs; whereas these neutral HBPs were eventually cleared in the urine, charged HBPs mainly accumulated in liver and spleen. Overall, these results demonstrate that, regardless of surface charge, HBPs display a high level of haemocompatibility. In contrast, immunoreactivity and biodistribution are significantly influenced by charge. This highlights that manipulation of surface charge may thus be a useful method by which nanomaterials such as HBPs can be tailored to different clinical applications.

Developing important CBNS standards to predict in vivo behaviour of nanoparticles An important centre-wide initiative is to improve the way we study nanomaterial and cell interactions[4]. This has important implications for our work with primary blood immune cells. As world leaders in this space, it is important we set excellent examples and this is now being practised across a range of current and future publications. This will ultimately advance the field. As systems become better standardised, our efforts to computationally model nanoparticle-immune cell interactions will be accelerated across a range of both CBNS-generated systems and systems generated by our partners and the whole field.

Figure 2. Study of hyperbranched polymers in human blood and in mice.

Key goals for 2019 1 Expand measurement and model-based analysis of the interaction of nanomaterials with blood and with immune cells in whole blood, across a range of nanoparticles with varying size, charge and surface chemistry available through the Centre (performed in concert with computational biology/modelling investigators)

2 Develop informative models of how nanomaterials interact with immune cells in lymph nodes and other immune organ systems 3 Better understand the role of blood plasma proteins in influencing nanomaterial interactions with immune cells

References: 1. Wang M., Gustafsson O., Siddiqui G., Javed I., Kelly H., Blin T., Yin H., Kent S.J., Creek D., Kempe K., Ke P.C., Davis T. Human plasma proteome association and cytotoxicity of nano-graphene oxide grafted with stealth polyethene glycol and poly(2-ethyl-2-oxazoline). Nanoscale 2018; doi.org/10.1039/c8nr00835c. 2. Song D., Cui J., Sun H., Nguyen T.H., Alcantara S., De Rose R., Kent S.J., Porter C.J.H., Caruso F. Templated Polymer Replica Nanoparticles to Facilitate Assessment of Material-Dependent Pharmacokinetics and Biodistribution. ACS Applied Materials and Interfaces. 2017; 9: 33683-33694. 3. Chen L., Glass J.J., De Rose R., Sperling C., Kent S.J., Houston Z., Fletcher N., Rolfe B., Thurecht K. The influence of charge on hemocompatibility and immunoreactivity of polymeric nanoparticles. ACS Applied Bio Materials 2018;1:756-767. 4. Faria M., BjĂśrnmalm M., Thurecht K., Kent S.J., Parton R., Kavallaris M., Johnston A.P.R., Gooding J., Corrie S., Boyd B.J., Thordarson P., Whittaker A., Stevens M., Prestidge C., Porter C., Parak W., Davis T., Crampin E., Caruso F. Minimum Information Reporting in Bioâ&#x20AC;&#x201C;Nano Experimental Literature. Nature Nanotechnology; 2018;13:777-785.

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Influence of hyperbranched polymer nanoparticles on immune reactivity in whole human blood

Strategic Projects In addition to the core research undertaken in the signature and overarching projects and the Centre-wide research, CBNS provides funding for activities that meet the strategic objective of the Centre to increase collaboration whilst extending the research capabilities. Strategic Projects are assessed by all Chief Investigators and are supported by a dedicated fund. The strategic funding is managed centrally and all collaborating organisations contribute part of their ARC funding each year. When assessing proposals for strategic funding, the CBNS Executive Committee considers whether the projects: • Involve collaboration between at least two CIs or members of their research groups

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• Involve collaboration between at least two nodes • Introduce skills, expertise or activities not presently available within the Centre • Have a duration no longer than two years (although after two years, successful projects can apply for additional funding to continue the project)

• Address at least one non-research KPI (such as media, public or industry engagement, visitors, training, etc.) • Not exceed an annual cost of $100,000 In addition to small amounts of funding supporting student attendance at workshops, two major projects were funded in 2018. They are detailed in the following section.

Open Data Fit Leader: Professor Pall Thordarson (UNSW)

The Open Data Fit project1 is creating a portfolio of websites that bring together tools and information of relevance to CBNS as well as the wider international research community. The project focuses on what we call small-scale data, i.e. the small data sets that are the bread-and-butter of research in bio-nano science research, ranging from kinetic measurements (kinetics) and small-angle scattering curves (nanoparticle characterisation) to cell viability measurements (IC50 for drug treatments). Currently, there are no universal tools that encourage scientists to share small-scale data effectively, even when the results are published. This data from well-designed and

well-executed experiments that are a part of unsuccessful or unfinished research programs are in most cases never shared with the broader community, becoming what is called dark data2, languishing forever in the (digital) archives of individual laboratories around the world. The goal of the Open Data Fit is therefore, to shine a light on smallscale dark data and transform the way scientists share these datasets. To this end, we designed the Open Data Fit project to assist researchers with their everyday work while at the same time capturing the raw data for effective sharing. The opendatafit (http:// opendatafit.org) web portal (tools) we are building will achieve this by allowing

the end-user scientist to upload their data and then perform data fit (analysis) on these datasets, while at the same time capturing the raw data, the analysis method and results simultaneously in an open-access database. The end-user experience is explained further in this youtube video: https://www.youtube. com/watch?v=Gc3f85Hhclw) With CBNS support, we built and successfully launched our pilot website supramolecular.org in 2015. This website provides tools for end-users to determine binding constants from NMR, UV-Vis or fluorescence titration data. Since it was launched, it has had over 28,000 visits with the average session lasting 3:30 minutes, showing a high level of engagement from end-users. Moreover, the website has been cited 80 times in the literature (Scopus). Significantly, Sir Fraser Stoddart, Nobel Prize Winner in Chemistry in 2016, agreed to become the Patron of this project. In 2018, we worked on building “version 2” of the platform. This followed on from us obtaining a $150,000 Research Infrastructure Scheme Grant from UNSW for 2018 to continue with the expansion of the open data fit platform. Version 2 will be based on a newer, much more modular framework. As part of this, we are also constructing new modules to analyse rate constants (kinetics) and IC50 data with flow cytometry data soon to follow. We plan to release the first modules on the new Version 2 platform in the first half of 2019 and, with the now approved cash support from CBNS, then commence on the flow cytometry analysis module in close collaboration with Matthew Faria from the Caruso Melbourne group. The new framework and the IC50 + kinetic modules are entering “alpha” testing at the end of 2019.

1. Hibbert D. B., & Thordarson P., The death of the Job plot, transparency, open science and online tools, uncertainty estimation methods and other developments in supramolecular chemistry data analysis Chemical Communications 2016, 12792-12805. 2. Björnmalm M., Faria M. & Caruso F., Increasing the Impact of Materials in and beyond Bio-Nano Science J. Am. Chem. Soc., 2016, 138, 13449-13456.

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Co-Leaders: Professor Tom Davis, Professor Ben Boyd and Dr Simon Corrie (Monash), Professor Edmund Crampin (Melbourne), Professor Maria Kavallaris (UNSW)

JTCC: visualising bio-imaging and data in 3D virtual reality (VR) Leaders: Associate Professor John McGhee (UNSW), Professor Tom Davis (Monash) Co-Leaders: Dr Andrew Lilja, Mr John Bailey, Mr Campbell Strong, Dr Rowan Hughes and Dr Shereen Kadir (all UNSW)

2018 progress The capacity of the 3DVAL was boosted early in 2018 with computer scientist Dr Rowan Hughes joining the team, allowing an increased focus on the development of largely-automated computer graphics pipelines for realtime exploration of imaging data in VR. Also, the onboarding of Dr Shereen Kadir, in late 2018, as a biomedical 3D animator and science communicator provided the lab with real breadth across the science and computer arts. Related projects, including the VR visualisation of in vivo nanoparticles and multi-user data analysis platforms continued as core areas of the Signature Project, with an emphasis on user testing and evaluation as science discovery and education tools.

Sub-projects: Voxcells: real-time volumetric approaches to visualise cell data (UNSW, UQ and Monash) Visual inspection of volumetric datasets (as acquired by MRI, CT, PET, LLS, FIB, etc.) is a fundamental activity that develops our understanding of biological structure, function, and relationship.

This project aims to re-think the standard approaches to volume rendering, to provide both investigators and the lay viewer with a new look at on volumetric datasets. This project also tackles some problems associated with rendering and lighting data sets of this size at real-time rates. To date, we have built a VR data inspection tool with several performance innovations. We have also developed an innovative lighting algorithm that allows for much more detailed and realistic lighting in a real-time environment. Visualisation of high-resolution microtubule and cellular dynamics data (UNSW, CCIA) This project visualises multiple, complex, dynamic cellular imaging Light Sheet lattice data sets provided from the UNSW node in an arena-like environment for immersive comparative analysis. The project has been built with a backend that supports multi-user interaction and exploration of the data which enable long-distance collaboration and cooperation. Additionally, it features the ability to manipulate and freely examine the data sets (including temporal changes), remote voice chat and personalised VR avatars.

3D VR of in vivo nanoparticle dynamics data (UNSW, UQ) This project aims to visualise CT and PET data in VR to identify the precise number and location of particles following in vivo administration. The research explores how design-led VR can be used as a platform to understand and interact with pre-clinical imaging data in an immersive and intuitive manner. The prototype, which includes features for isolating organs and particle-sampling tools, continues to be refined following several rounds of user testing and will be formally evaluated in 2019 for science discovery and educational applications.

Publications: Lilja A., Strong C., Bailey B. J., Thurecht K., Houston Z., Fletcher N., McGhee J., Design-led 3D Visualization of Nanomedicines in Virtual Reality, 24th ACM Symposium on Virtual Reality Software and Technology (VRST â&#x20AC;&#x2122;18), November 28-Dec 1, 2018, Tokyo, Japan.

Press: 23 Oct 2018, Chanel 10 News Adelaide CBNS roadshow: JTCC VR Oculus GO experience for high school students

Key goals for 2019 1 Refine volumetric data processing pipelines for enhanced forms of 3D image visualisations on VR head-mounted displays 2 Refine and test meaningful and embodied interfaces that allow remote multi-user immersive 3D VR data interaction

Figure 1: Voxelised data visualisation of liver tissue. Image Credit: John McGhee, Campbell Strong, Rowan Hughes, Rob Parton

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3 Evaluate the effectiveness of immersive VR as a platform to visualise and interact with nanoscience laboratory data compared to traditional screen-based methods 4 Generate data-informed explorable digital landscapes that unravel the complexities of nanoscience. Posing visual hypotheses, representing the invisible and gaining more insight in to the art-science dialogue and co-development process

Figure 2: Artistic visualisation of PET-CT imaging data. Image credit: John McGhee, Andrew Lilja, Campbell Strong, Kris Thurecht

R e s ear c h Figure 3: Collaborative virtual spaces and avatars enable socially-driven data analysis. Credit: John Bailey, John McGhee, Maria Kavallaris.

Figure 4: Visualisation of in vivo nanoparticle distribution in virtual reality. Interactive heads-up displays allow the user to navigate through the complex data set. Image credit: John McGhee, Andrew Lilja, Campbell Strong, Kris Thurecht

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Engagement 36 CBNS Annual Report 2018

e n gag e m e nt

Engagement CBNS Annual Report 2018 37

Collaborations and Partnerships CBNS researchers have a long history of collaboration with other universities, research centres, industry and medical organisations. These collaborations take a wide variety of forms – from formal relationships such as Linkage Projects or contract research, to informal and ad hoc collaborations.

Intellectual Property and Commercialisation Policy The CBNS IP and Commercialisation Committee (IPCC) provides advice for IP management to CBNS Chief Investigators. The IPCC comprises the Centre Executive (Director, Deputy Director and Manager), plus the Business Development Manager from the Administering Organisation (Monash). In discussing IP from collaborating organisations, the IPCC co-opts the Business Development Manager (or equivalent) from that organisation.

Intellectual Property

Commercialisation

CBNS does not own project intellectual property (IP) resulting from CBNS activities. Project IP is owned by the (administering, collaborating or partner) organisation making the major inventive contribution to the IP. Whilst a single organisation assumes control of project IP, in the cases of cross institutional participation in project IP generation, beneficial interest in project IP, is determined by relative contributions to IP generation based on cash and in-kind contributions to that project (cash is weighted by a factor of 2).

The organisation owning the project IP supports protection and commercialisation through its IP commercialisation group and meets the cost of protection.

CBNS maintains an Intellectual Property Register, which includes up to date details of CBNS IP and each organisation’s background IP. It consists of: • the owners of each item of background and IP and the proportion of ownership • any and all third party interests in background and IP • any patents or patent applications relating to

38 CBNS Annual Report 2018

CBNS commercial partners provide real world advice to Centre Chief Investigators but also provide a potential path-to-market and a defined route to impact. As such, industry partners have the right of first refusal to a license to exploit project IP on terms to be agreed.

In relation to the commercialisation of project IP, all parties negotiate in good faith to agree on a Commercialisation Lead and the terms of any Commercialisation. The Commercialisation Lead has the right to sublicense project IP in the interests of all parties and CBNS, without restricting the use of the project IP for research, teaching and scholastic endeavours.

Patent applications, invention disclosures and selected commercial activities Node Type

Date

Inventors/Researchers involved

Monash US provisional application (filed) 02 Aug 2018

Chris Porter, Daniel Bonner, Ketki Karanam, James Mutumba, Rishab Shyam, Sifei Han, Luojuan Hu, Tim Quach, Natalie Trevaskis, Jamie Simpson

PCT application 29 Aug 2018

Chris Porter, Daniel Bonner, Ketki Karanam, James Mutumba, Rishab Shyam, Jamie Simpson, Sifei Han, Luojuan Hu, Tim Quach, Natalie Trevaskis

US provisional application (filed) 16 Nov 2018

Chris Porter, Daniel Bonner, Sifei Han, Luojuan Hu, Ketki Karanam, Tim Quach, Rishab Shyam, Jamie Simpson, Natalie Trevaskis

US provisional application (filed) 29 Aug 2018

Chris Porter, Daniel Bonner, Sifei Han, Luojuan Hu, Ketki Karanam, Tim Quach, Rishab Shyam, Jamie Simpson, Natalie Trevaskis

US provisional application (filed) 29 Aug 2018

Chris Porter, Daniel Bonner, Sifei Han, Luojuan Hu, Ketki Karanam, Tim Quach, Rishab Shyam, Jamie Simpson, Natalie Trevaskis

Provisional application

22 Oct 2018

Tom Davis, Nik Veldhuis

UNSW

Australian provisional application

24 Jul 2018

Justin Gooding Maria Kavallaris, Aidan Patrick Oâ&#x20AC;&#x2122;Mahony, Robert Hadinoto Utama, Christopher Michael Fife, Lakmali Atapattu, Julio Cesar Caldeira Ribeiro, Maria Kavallaris, Justin Gooding

UniSA

US complete application (published)

05 Apr 2018

Clive Prestidge, Paul Joyce

Australian provisional application

07 Feb 2018

Benjamin Thierry

Melbourne PCT application

20 Sep 2018

Frank Caruso

PCT application

20 Sep 2018

Frank Caruso

US complete application (published)

n/a

Kris Thurecht

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US complete application (published) 16 Aug 2018

CBNS Annual Report 2018 39

Industry and commercial engagement CBNS researchers have a range of collaborations with commercial organisations. These relationships are typically led by one of our Chief Investigators and link with Australian and international biotech experts. The collaborations provide an opportunity for bio-nano research to be translated into product development and, ultimately, application and use. Examples include: Main Contact

Company

Content

Associate Professor Aegros International Kris Thurecht

Smart affinity membranes for advanced manufacturing of high value therapeutic proteins

Professor Chris Porter

Capsugel

Ionic liquid technology for drug delivery

Professor Benjamin Thierry Professor Clive Prestidge

Ceramisphere Ltd. (ARC Linkage)

Development of an in vitro model of the intestinal epithelium to optimize oral nano-vaccine

Professor Benjamin Thierry Ferronova Ltd. - funded by Improving head and neck cancer staging with nanoparticles TechinSA Professor Ben Boyd Galera Therapeutics

Understanding lipid and colloidal interactions with GC4419 and analogs

Professor Ben Boyd

3D printing of self-nanoemulsifying formulations

Gattefosse

Professor Chris Porter Halozyme Therapeutics

Perturbation of the extracellular architecture to promote the absorption and lymphatic transport of biological macromolecules

Professor Pall Thordarson

Lleaf Pty Ltd (ARC Linkage 2017-2020)

Precision luminescent solar concentrators from robust quantum dot arrays

Professor Benjamin Thierry

Murdoch University, WaterNSW, Seqwater and SA Water

Development and validation of rapid detection and viability assays for Cryptosporidium, Norovirus, and Adenovirus and using a novel gut-on-chip

Professor Benjamin Thierry RHS Ltd and Repromed

Development of a novel non-invasive prenatal testing technology based on circulating fetal cells

Professor Ben Boyd Sigrid Therapeutics Understanding the effect of SiPore on lipid digestion and absorption Professor Chris Porter Dr Angus Johnston

StarPharma

Novel targeted delivery systems

Associate Professor Kris Thurecht

StarPharma

Imaging the distribution of dendritic nanomedicines

Professor Chris Porter

PureTech Health

Targeting the lymphatic system using glyceride-mimetic prodrugs

Associate Professor Telex Pharmaceuticals Kris Thurecht

Biodistribution, pharmacokinetics and efficacy of therapeutic antibodies

Professor Pall Thordarson

Scaling manufacture of 3-D microstructures for the medical devices industry

Zhizhen Medical Co (ARC Linkage 2018-2020)

Further details on the range of industry and commercial engagements of CBNS can be found on our website: https://www.cbns.org.au/outreach/industrial-and-commercial-engagement/

40 CBNS Annual Report 2018

Health and medical sector engagement CBNS researchers have a range of links with organisations in the health and medical sector. These relationships are typically led by one of our Chief Investigators and link with hospitals and medical research institutes. The collaborations provide an opportunity for bio-nano research to be undertaken in collaboration with medical researchers and practitioners, informed by end user needs. Main Contact

Company

Content

Professor Stephen Kent

Aarhus University Denmark

Clinical trials of approaches to cure HIV

Professor Clive Prestidge

Boulos & Cooper Pharma

Preclinical development of the novel antibiotic Ramizol

Professor Stephen Kent

Burnet Institute Melbourne

Novel molecules to measure immune responses

Professor Clive Prestidge

Holman Pharma Pty Ltd

Improving oral delivery of poorly permeable drugs

Professor Stephen Kent

Imperial College London UK

Clinical trials of new HIV vaccines

Professor Benjamin Thierry Massachusetts General Development of bioengineered in vitro tumour models for Hospital & Harvard Medical radiobiological studies School Professor Stephen Kent Monash University (Clayton)

New ways of targeting immunity to HIV (also with Centre for Advanced Molecular Imaging )

Professor Stephen Kent National Institutes of Health, USA

New antibodiy prepartions to treat Influenza

Professor Clive Prestidge

Reform Pharm Pty Ltd

Lipoceramic reformulation of cardiovascular drugs

Professor Benjamin Thierry Royal Adelaide Hospital

Smart “Nano Tracer” for improved preoperative staging with MRI and PET

Professor Benjamin Thierry

Royal Adelaide Hospital

Clinical trialling of Ferronva’s staging technology

Professor Andrew Whittaker

Singapore Bioimaging Consortium (A*STAR)

Cell uptake and imaging

Professor Benjamin Thierry

Stanford Cancer Institute

Development of nanomedicine molecular PEG tracer

Professor Stephen Kent The University of California, Novel influenza vaccine Irvine Associate Professor Kris Thurecht University of Birmingham

Polymeric imaging agents

Professor Stephen Kent

Vanderbilt University, USA

New antibodies to treat Influenza infection

Dr Adam Wheatley Professor Stephen Kent

Vaxxas

Novel analyses on immune responses induced by nano patch

Further details on the range of industry and commercial engagements of CBNS can be found on our website: https://www.cbns.org.au/outreach/health-and-medical-sector-engagement/

CBNS Annual Report 2018 41

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Associate Professor Kris Thurecht PA Hospital, Metro South, Brain Cancer Brisbane

Collaboration with partner organisations CBNS engagement with our partner organisations varies as research activities gain in focus. In 2018, there has been a research visit from CBNS Alumna Dr Amanda Pearce from the University of Nottingham. She visited UQ and Melbourne as well as UNSW in January and February. Associate Professor Weibo Cai from the University of Wisconsin-Madison visited both Melbourne and Monash in April and Dr Jason Lewis from Memorial Sloan Kettering Cancer Centre also came to Australia. Australian CBNS researchers have travelled to our local and overseas partners to undertake collaborative activities. CBNS members Ms Ava Faridi and Mr Marvin Wang (both Monash) visited Tongji University (Shanghai, China) over a period of one month. CBNS and Tonji University are collaborating research partners and Ava and Marvin had the

42 CBNS Annual Report 2018

chance to use their zebrafish facility under the supervision of Dr Sijie Lin. Dr Lin is Professor and Principal Investigator at Lin Lab. Both Marvin and Ava went to Tongji University to gain some international research experience and plant the seed for more international collaborations. Marvin said he was extremely excited to work in Dr Lin’s research facilities after fellow student Ibrahim Javed (Monash) recommended the high-end zebrafish lab he was able to work in during his visit. Ava was keen to enhance the depth of her experiments at the huge and modern zebrafish facility at Tongji University. And they were not disappointed. Ava and Marvin spent most of their short time in the lab focusing on their projects. Ava is looking into the effect of nanoparticles on inhibition of protein aggregation related to type 2 diabetes. One of the advantages of the Lin Lab is that processes can be carried out much faster and more efficient because

“you don’t have to wait to use the lab like at home”, Marvin says. Collaborating with and working in an environmental department was completely different to what both Ava and Marvin are used to at their home institution. “But I was most proud of my time management skills and that my plan worked out despite the small time frame”, Ava says. Due to their limited time at Tongji University, both Ava and Marvin had to carefully plan their lab time and project.

“Use the opportunities and the support CBNS provides. Don’t miss those valuable chances just because you think you are too busy.” Both wanted to express their appreciation to CBNS and Tongji University to make this lab visit an unforgettable experience.

CBNS Experience One of the advantages for CBNS members is the opportunity to gain valuable research experience outside of their home institute. CBNS offers its PhD students the opportunity to visit other labs and present their research at external conferences and events. In this section, three PhD students and two alumni share their experience from 2018.

PhD Students Increase your research exposure and foster relationships In July 2018, UniSA PhD students Chelsea Thorn and Hayley Schultz toured the USA and Canada, showcasing their research. The trip was funded by Hayley’s 2018 CBNS Student Travel Award and UniSA Research Degree Excellence Grant, and Chelsea’s Young Pharmacist Professional Innovation Grant.

Chelsea says, “The trip to North America was a fantastic opportunity to increase our research exposure and foster relationships with collaborators. The bonus of interacting with the pharmaceutical industry has also given me a sound insight into potential career prospects.” Hayley says, “We’ve come back to Australia with a greater understanding of how research is conducted in North America in both an academic and industry setting. We now realise that our skills are actually very applicable to industry and that it is a very possible career pathway for us to consider.” Both students have gained significant interest in their PhD projects as well as numerous connections for future career prospects in both academia and industry.

PhD students Chelsea Thorn and Hayley Schultz

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Together, Chelsea and Hayley visited two research groups (Professor Hageman at Kansas University in Lawrence and Professor Howell at SickKids Research Institute in Toronto) and three pharmaceutical industry sites (Pfizer in Groton, Bristol Myers-Squibb in New Brunswick and Princeton), as well as attend the four-day Controlled Release Society conference in New York. They had the opportunity to present their 30-minute talks to the research groups and industries (a total of five talks) and a poster presentation at the conference. They received lots of helpful advice and words of encouragement on their research projects and praise on their initiative to tour the USA and Canada.

UNSW’s Kristel Cahyadi Tjandra

Embracing the opportunity to be part of a research collaboration program The 2018 CBNS Student Travel Award has helped fund Kristel’s trip to a conference in Rome, which she attended at the end of her PhD. The conference was called the “International Conference on Nanomedicine and Nanobiotechnology (ICONAN)”. Being the first European conference Kristel has ever attended, “it was really a great experience for me to be able to get to know some of the scientific community in Europe”, she says. Because of the conference size of roughly 300 delegates, she was also able to network with other PhD students and ECRs from various European institutions. The conference was strategically arranged so that delegates could spend time together for meals and social activities. At the conference, Kristel was also given the opportunity to present her PhD work in the form of an oral presentation and through that she has received many encouraging feedback and suggestions. Kristel says for her it was not only a great learning opportunity but also a rewarding experience to be able to share her knowledge with those who are mutually enthusiastic about the field. During the same travel, she also had the chance to visit other parts of Europe. This made the trip a valuable working vacation and a time for her to wind down before she jumped on her Postdoctoral role at UNSW.

CBNS Annual Report 2018 43

Dr Lars Esser with the research group at Monash

Alumni Dr Lars Esser, Monash University now CSIRO Lars completed his PhD with Centre Director Professor Tom Davis at the Monash University node of CBNS in 2017 and continued as a postdoc for one year. His research was focused on developing polymer-based nanoparticles for applications in drug delivery and diagnostics. During this time, he was also a member of the Outreach SubCommittee and co-organised an event for the National Science Week during which the award-winning documentary Jabbed – Love, Fear and Vaccines was shown. The screening was followed by a discussion panel and Q&A session with experts in the fields of immunology and public health. In March 2018, Lars moved on to CSIRO where he is now working on a theranostic nanoparticle-based approach to treat brain cancer. As CSIRO has a three-year postdoc program, this has given Lars the opportunity to fully immerse in this new project, including talking to clinicians to understand the limitations of current therapies and how these could potentially be overcome. Over the next two years, he hopes to show some promising in vivo data and have a contribution to the improved treatment of glioblastoma. Next to this, he is trying to establish himself as an independent researcher and to increase his network via new collaborations, the CBNS alumni network and Twitter. “Fortunately, I still am a little bit involved with CBNS-investigators too. For example, I work closely with Simon Puttick and I’m also collaborating with Simon Corrie on a Monash Institute of Medical Engineering project”, Lars says.

44 CBNS Annual Report 2018

Dr Jonathan Wojciechowski, University of New South Wales now Imperial College London Jonathan completed his PhD in Supramolecular Chemistry under the supervision of CBNS CI Professor Pall Thordarson at UNSW. He was inspired by complex systems found in nature, particularly by the high level of self-organisation which could impart seemingly simple components (i.e. peptides) with function (e.g. enzymes). “My PhD within CBNS gave me key skills to synthesise, characterise and understand the self-assembly of short peptides and how changes in chemical structure affected their functional properties. Whilst we achieved the design of a transient supramolecular system in my PhD, their resultant properties are infant compared to nature,” Jonathan says.

The ability of materials such as the extracellular matrix to orchestrate various mechanisms such as tissue regeneration fuelled his curiosity. After his PhD, Jonathan joined the group of Professor Molly Stevens at the Centre’s Partner Organisation Imperial College London, working on biomaterials with applications in regenerative medicine and tissue engineering. Jonathan hopes to use his time in the Stevens Group to further his understanding of biomaterials and understand how we can develop materials with similar spatiotemporal dynamics of natural biomaterials.

Events Events provide an excellent opportunity to share important research, form new collaborations and strengthen existing ones. CBNS delivered another series of successful events during 2018. Here are some of the highlights.

The 2018 CBNS Annual Research Workshop took place at the Crowne Plaza Terrigal Pacific at New South Wales’ Central Coast from 28 to 30 November 2018. Despite adverse weather delaying the start, this year’s workshop was a huge success. Over 100 delegates shared their research achievements and challenges they faced during 2018. Each CBNS node presented updates on their current work, the life at their respective nodes and discussed future research opportunities. Furthermore, a special career event enabled CBNS members to ask probing questions and engage in discussions with experienced and successful scientists from inside and outside academia. Career Advisors were on hand to assist members evolve their thinking and make a significant leap forward in their own careers. There was the opportunity to talk with other members or CIs about ideas and potentially start new collaborations towards new career paths. During the CBNS Annual Research Workshop, we recognised members for the most significant publication of 2018, the best poster presentation as well as the winner of the Science by Design Competition. The workshop also included recognition of the 2018 CBNS Student Travel Award winners (see page 51 for details). A joint meeting of the CBNS Education Committee and the Outreach SubCommittee facilitated discussions and feedback for events, training and activities that could take place during 2019. Both committees’ programs and activities during 2018 were positively received by members at the workshop. This has resulted in a range of beneficial

activities that not only build the capacity of CBNS members but also provide a platform to demonstrate the Centre’s important work to the public.

International Nanomedicine Conference CBNS was once again the co-host with UNSW of the Australian Centre for Nanomedicine’s International Nanomedicine Conference. This was the 9th annual conference to be held at Coogee in New South Wales. The conference brought together Australian nanomedicine researchers from medicine, chemistry, engineering and sociology, with international experts. A feature each year is the opening plenary talk delivered by the CBNS Visiting Professor. In 2018, Professor Warren Chan from the University of Toronto, Canada presented on the Nanoparticle Delivery to Solid Tumours. In addition to the talks and poster sessions the conference is a confluence of national and international expertise and most CBNS researchers attended. The CBNS Scientific Advisory Board meets during this event, with expert input and advice from the plenary speakers each year.

2018 Visiting Professor: Warren Chan Our annual Visiting Professor program brings outstanding international scientists to Australia to advance bio-nano science. In 2018, Professor Warren Chan Distinguished Professor in the Institute of Biomaterials and Biomedical Engineering at the University of Toronto, visited Australia. His research interest is in the development and translation of nanotechnology for diagnosing and treating cancer and infectious diseases. He has received the NSERC E. W. R. Memorial

Steacie Fellowship, Kabiller Young Investigator Award in Nanomedicine, the BF Goodrich Young Inventors Award, Lord Rank Prize Fund award in Optoelectronics (England), and Dennis Gabor Award (Hungary). Currently, he is an Associate Editor of ACS Nano. Professor Chan visited Australia from 25 June to 10 July 2018. During this time he visited all five nodes where he delivered research seminars and met with the CBNS CIs, ECRs and students. As part of the Visiting Professor program, Professor Chan attended the International Nanomedicine Conference and gave the opening plenary talk as well as participated in the CBNS Scientific Advisory Board. The Visiting Professor in 2019 will be Chunying Chen, Principal Investigator at the Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology in China.

ARC Joint CBNS and CLEX Media Training In August, we organised an ARC Joint Media Training together with the ARC Centre of Excellence for Climate Extremes (CLEX). The unique media training workshop allowed delegates from both Centres to develop confidence and skills for interviews with the media and the general public. It was designed to help ECRs and students to acquire the skills to get their research message across a wide range of audience. A special feature of the workshop was the collaboration of two very different research fields simulating interviews on their respective research in mixed working groups. The learnt skills are also valuable in preparing for job interviews, fellowship applications, delivering presentations or even writing articles. The workshop was a huge success for both Centres with new collaborations and interdisciplinary networks forming. In 2019, we will extend the workshop and include the ARC Centre of Excellence for Mathematical and Statistical Frontiers (ACEMS) to gain an even broader audience and more complexity in the preparation for an interview.

CBNS Annual Report 2018 45

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CBNS Annual Research Workshop

Frontiers in Bio-Nano Science This year marked the inaugural year for the first student and early to mid-career researcher (EMCR) run conference for CBNS members. Frontiers in Bio-Nano Science (FBNS) 2018, a one-day event co-badged event organised by members of CBNS and BLiSS (http://www. bliss-ecr.org/), was hosted by Monash Institute of Pharmaceutical Sciences at Cossar Hall on 27 September. FBNS is a final year PhD student and EMCR focused event for cross-disciplinary networking and collaboration and serves as an open and safe environment for presenters and attendees to engage others about their research, form new partnerships and gain insight on how to translate their ideas into innovations. The program was designed to tackle these issues and challenge participants to become active members of the discussion throughout the day. It comprised of two plenary speakers, five panel members, seven innovators for a discussion forum, 12 oral speakers and 13 poster presenters. The first plenary, CBNS CI and Node Leader Professor Justin Gooding (UNSW) set the tone of the day with an inspirational talk about the highs and lows of his journey, advice for overcoming setbacks and challenges and motivation to remind everyone why they are worthy and uniquely placed for a career in academic science. Oral sessions throughout the day were structured for 20 minutes of questions in order to allow for open discussion and generation of new ideas. The afternoon plenary featured Dr Marguerite Evans-Galea, the Executive Director of Industry Mentoring Network in STEM (IMNIS), co-founder and CEO of Women in STEMM and co-founder of the EMCR Forum. Dr Evans-Galea not only gave her personal story and journey, she also painted the difficult reality of what success will mean for postdocs in the modern era of science and copious advice on how to overcome those hurdles. Closing the day was a panel on “How to take research ideas and collaborate with industry to make successful innovations.”

46 CBNS Annual Report 2018

The panel included five experts of varying backgrounds and expertise in academia, industry, and business: CBNS CI Professor Stephen Kent – University of Melbourne Dr Richard Huysmans – Raven Consulting Group Dr Anna Lavelle – Avatar Brokers Professor Darren Martin – University of Queensland Professor Jan Tennent – Biomedical Research Victoria Dr Cathy Drinkwater – Biocurate Pty Ltd Dr Cameron Ferris – Inventia Life Science Dr Chris Langmead – Monash University Dr Kathy Nielsen – Monash University Prof Chris Porter – Monash University Dr Robert Utama – UNSW Sydney and Inventia Life Science Dr Bo Yun – Peter MacCallum Cancer Centre At the end of the formal program, participants had the opportunity to apply their knowledge from the day and network and ask questions to seven innovators. Overall feedback for the event was highly positive from the participants, plenaries, panellists, innovators and presenters. Most importantly though, the positive response received from the steering committee had the largest impact on success of FBNS 2018 and the future of the event. The goal of FBNS 2018 was not only to put on a thought-provoking and inspiring event for the PhDs and EMCRs of CBNS, it also provided a learning opportunity for those who wanted to learn how to develop a program and event that answered a need and provided a benefit

for others to aspire towards scientific and career excellence. The committee was responsible for developing the program, inviting speakers, coordinating a workshop for oral presenters, researching and planning a panel, obtaining sponsorship and managing the operations of the event. It was a large team effort and was a huge success due to its collaborative effort from everyone involved. The event would not have been possible without the following people: Dr Simona Carbone – Program Co-Chair (Monash) Dr Manish Sriram – Communications Co-Chair (UNSW) Dr Duy Phu Tran – Operations Co-Chair (UniSA) Dr Zachary H. Houston – Lead Coordinator (UQ) Dr Florence Cotel – BLiSS Founder and Liaison (formerly UQ) Dr Nicholas Veldhuis – Lead Coordinator (Monash) Ms Anne Meyer – Lead Coordinator (Monash) Committee Members

r Craig Bell (UQ) D Ms Ava Faridi (Monash) Ms Paulina Ramirez-Garcia (Monash) Dr Yi (David) Ju (Melbourne) Mr Song Yang Khor (Monash) Dr Samantha Kristufek (Melbourne) Ms Ayaat Mahmoud Mohamed (Monash) Ms Duyen Ngyuen (UNSW) Mr Pradeep Rajasekhar (Monash) Dr Priyank Shenoy (Monash) Volunteers

s Ewa Irena Czuba (Melbourne) M Ms Yiyuan Han (Melbourne) Mr Lei Huang (Melbourne) Dr Manasi Jambhrunkar (UniSA)

Education Committee The Education Committee’s objective is to identify the training and capacity building requirements of the members of the Centre. In a collaborative, inclusive and transparent manner, the committee designs, promotes, delivers and evaluates an annual series of education-based events.

The CBNS Education Committee met monthly during 2018 to assess and organise the training and education needs of the Centre.

The Strategic Academic This workshop offered early career academics and researchers a simple road-map to success. It guided them through the process of clarifying their career priorities, identifying their critical goals and addressing their time wasters. The workshop steered the participants to a more strategic approach to academic career success. They learnt to understand the challenges of the complex world of the academic.

2018 Education Committee events: Event title

Date Node

Fiji/ImageJ Workshop

4 to 6 Apr

Monash

Scientific Presentation Workshop

28 Jun

UNSW

Adobe Illustrator Training

12 to 13 Jul

Open House Melbourne*

28 Jul

Monash

CBNS and CLEX Media Training*

6 Aug

UNSW

Frontiers in Bio-Nano Science Conference

27 Sep

Monash

The Strategic Academic

27 Nov

UNSW

Nailing Grants, Papers, Prizes and Awards

27 Nov

UNSW

*Joint Education and Outreach Sub-Committee event

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The Education Committee is chaired by CBNS CI Professor Maria Kavallaris (UNSW). The committee is formed of members from across all nodes with each node being represented by a postdoctorate researcher and a PhD candidate.

Illustrator Workshop Nowadays, a researcher can no longer rely on the power of their words to convey a convincing scientific argument – information in the form of diagrams play just as important a role. Being confident in one’s own ability to produce such diagrams is a fundamental skill. Frontiers in Bio-Nano Science

This workshop was specifically designed for scientists to help them understand basic graphic design software such as Adobe Illustrator. During the training, participants developed skills through step by step learning and practice-based projects over a 2-day program.

Scientific Presentation Skills Workshop Delivering a scientific presentation that captivates and persuades a broad audience often seems easier said than done. This one-day workshop helped delegates to boost their skills and confidence to prepare effective presentations and deliver better results. It provided a new perspective on presentation principles, the use of figures and tables as well as delivering the presentation. The 18 delegates from

Illustrator Workshop

our five nodes learned how to plan their presentation, organise their story and define their audience. The workshop enabled them to adopt new techniques to plan the concept of their talk.

In practical sessions, participants trained how to deliver a presentation, inspire any audience and better convey their idea.

CBNS Annual Report 2018 47

Mentoring Program Mentoring relationships support the professional and personal growth of mentees through the development of new skills, finding new approaches to old problems, identifying areas for professional growth and accessing a wider professional network. These strategies are provided through the guidance or sponsorship offered by a mentor. Similar to the previous year, mentors are chosen and matched with mentees based upon their relevant skills, knowledge, experience or influence. Through the mentor-mentee relationship, the mentor may assist the mentees in developing future career goals and defining a framework to assist the mentee in reaching those goals. Mentoring can be beneficial in skill development, career and goal planning, developing successful networks, confidence building, developing managerial/leadership capabilities and understanding organisational structures. In 2018, we carried out two rounds of our mentoring program: one in the

National Science Week

48 CBNS Annual Report 2018

first half of the year and the second commencing in September 2018. The CBNS-led mentoring program is a formal mentoring arrangement and is designed for Early Career Researchers (ECR) and Mid Career Researchers (MCR). The mentor-mentee relationship may last through the progression of the menteesâ&#x20AC;&#x2122; current postdoctoral research project. Through the development and progression of the mentorship, the interaction can either focus on a particular challenge or concern or on broad career questions. The agenda is led by the mentee â&#x20AC;&#x201C; the mentor is there to facilitate, not influence decisions. CBNS continued with the same mentoring team as in 2017 to review the applications and try and match

each with a mentor â&#x20AC;&#x201C; either from within CBNS or external. The CBNS Mentoring Team is supported by CBNS CIs Professor Maria Kavallaris (UNSW) and Professor Stephen Kent (Melbourne) as well as Dr Natalie Jones (Centre Manager) and Dr Charlotte Hurry (Centre Administrator). While CBNS postgraduate students are not formally part of the mentoring program, support for students will continue to be provided by resources available at each node or institution. The Centre encourages students to interact with senior research leaders and ECRs at their local node and more broadly with CBNS members through regular research and training opportunities.

Outreach Sub-Committee The CBNS Outreach Sub-Committee is a sub-committee of the CBNS Education Committee. It is chaired by CBNS CI Associate Professor Matthew Kearnes (UNSW). The committee is formed of members from all nodes with each node being represented by a postdoctorate researcher and a PhD candidate. The Sub-Committee met monthly during 2018 to assess the outreach needs of the Centre and to coordinate a series of events across the nodes.

2018 Outreach Sub-Committee events: Event title

Date Node

Pint of Science Melbourne

14 May

Monash

Pint of Science Sydney

15 May

UNSW

Pint of Science Melbourne

16 May

Monash

Open House Melbourne*

28 Jul

Monash

CBNS and CLEX Media Training*

6 Aug

UNSW

The members foster a culture within CBNS of outward communication, in particular through public representations of the research that occurs and active use of social media channels.

Brisbane Science Festival – National Science Week

11 Aug

Super Drugs and Mini-Guts – National Science Week

16 Aug

UniSA

National Science Week

Science in the Cinema – National Science Week 19 Aug Melbourne Monash

National Science Week is Australia’s annual celebration of science and technology. Running each year in August, it features more than 1,000 events around Australia, including those delivered by universities, schools, research institutions, libraries, museums and science centres. This year, the Centre had four nodes contributing to National Science Week:

Brisbane Science Festival – UQ node Starting on 11 August, PhD students Ms Caterina Brighi and Mr Nick Westra van Holthe organised a booth at the Brisbane Science Festival showing hands-on experiments to members of the public. With over 5,000 people visiting the festival, the 14 CBNS volunteers were kept busy during the day making instant snow, creating macrocapsules using self-assembly techniques and producing nylon. A special highlight was the interactive VR demonstration visualising nanoresearch in a virtual reality.

CBNS Road Show 15 to 25 all nodes Oct *Joint Education and Outreach Committee event

Super Drugs and Mini-Guts – UniSA node UniSA’s Dr Marnie Winter and Ms Tahlia Meola organised a special exhibition in cooperation with UniSA’s Museum of Discovery (MOD) for local school students. The event provided a rare opportunity for students to speak with researchers (in total 12 CBNS PhD students and early-career researchers) about what it is like to study and work in STEM, as well as visit the recently opened MOD. Activities included the VR experience “Journey into the Centre of the Cell”, experiments on how small nano really is as well as an introduction into the nano-scale and nano-particles, focusing on their role in biomedicine. Moreover,

the volunteers demonstrated the making of a mini silicon intestine used to understand and mimic the conditions of the gut and the design of new smarter drugs which make the medication we take more efficient and more easily absorbed in our body.

Science in the Cinema – Melbourne/Monash node The week concluded with a free screening of the 1966 sci-fi classic Fantastic Voyage: A miniaturised crew attempt to heal their injured colleague by travelling through his body. But in 2018, do advances in science and technology mean that science fiction is becoming closer to reality? The screening of the film was followed by an expert Q&A panel session and an interactive demonstration of virtual reality equipment allowing participants to travel inside a cell. The panel included CBNS CIs Associate Professor John McGhee and Associate Professor Matthew Kearnes (both UNSW), Dr Christina Cortez-Jugo (Melbourne), Ms Kasha Singh (Peter Doherty Institute) and Dr Matt Faria (Melbourne) – MC for the panel. This event was organised by PhD student Hannah Kelly and Dr David Ju (both Melbourne) in conjunction with the Peter Doherty Institute.

CBNS Annual Report 2018 49

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The Outreach Sub-Committee identifies and promotes CBNS research to the public. It achieves this through community or industry-related annual series of outreach activities, events or media liaison. This includes working with the Education Committee to ensure strong collaboration and synergies occur across CBNS.

Pint of Science Over three days in May, three CBNS members presented their research as part of the Pint of Science 2018 festival. Pint of Science brings some of the most brilliant scientists to local pubs across the country, to discuss their latest research and findings with an interested audience. There is no prior knowledge needed and it is a valuable chance to meet the people responsible for the future of science. CBNS participation started with Dr Nik Veldhuis (Monash) entertaining a large audience at the Hawthorne Hotel and talking about the development of next-generation analgetics to target pain at the source. Dr Orazio Vittorio from UNSW presented CCIA’s project in nanoscience that could improve treatment for Australia’s deadliest and most drug-resistant cancer: pancreatic cancer. CBNS concluded their involvement with CI Professor Ben Boyd (Monash). He talked about the unique properties of milk and their impact on the effectiveness of anti-malarial drugs ultimately providing a cure for malaria. Ben also gave an interview the night before his talk on ABC Radio “Evenings with Lindy Burns” (https://www.abc.net. au/radio/melbourne/programs/ evenings/malaria-breakthrough/ 9768058 [15 May 2018, 7:00 pm])

Pint of Science

Each presentation provided perspectives on their respective research projects, the journey into a research career and training as a scientist following completion of high school. The program aimed to get students at an early stage to consider STEM courses and bio-nano science in particular. The talks were followed by interactive experiments that allowed the students to get an understanding of the bio-nano research undertaken by our Centre. Experiments included activities like coating of objects with metal-phenolic films: A simple experiment demonstrating the fast (less than one second) coating of everyday objects with a purple,

CBNS Roadshow During the course of two weeks, CBNS members visited five secondary schools at each node city to talk to students about the research undertaken at our Centre as well as the journey towards a career in research. The objective of this event was to provide a two-hour program for students in Year 9/10. The program included presentations from a senior research leader, an early career researcher and a PhD student.

Open House Melbourne

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School visits

10 nm layer of metal-phenolic film. This is achieved by simply dipping any object into a tannic acid solution followed by an iron chloride solution. Another activity included an interactive alginate experiment where participants quickly and easily prepared alginate ‘particles’ via the dropwise addition of an alginate solution into a CaCl2 crosslinker solution. This was an opportunity to demonstrate self-assembly techniques as well as explain ways how scientists prepare nanoparticles. The students were also able to experience the aforementioned VR experience.

CBNS Awards During 2018, members at all levels were recognised for their contribution to the Centre, as well as their hard work and dedication to science and pushing the boundaries of understanding in the area of bio-nano science. This section includes some of the highlights.

Most significant CBNS paper of 2018 (Student)

Dr Cheng Zhang (UQ)

Mr Matt Faria (Melbourne)

High F‑content perfluoropolyetherbased nanoparticles for targeted detection of breast cancer by 19F magnetic resonance and optical imaging (ACS Nano 2018, 12, 9162-9176)

Minimum information reporting in bio-nano experimental literature (Nature Nanotechnology 2018, 13, 777-785)

Dr Aleksandr Kakinen (Monash)

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Most significant CBNS paper of 2018 (Postdoc)

Winner Science by Design Competition 2018 (Judge’s and People’s Choice Award)

Most significant CBNS paper of 2018 (Director’s Choice)

Dr Roya Tavaille (UNSW) Nucleic acid hybridization on an electrically reconfigurable network of goldcoated magnetic nanoparticles enables microRNA detection in blood (Nature Nanotechnology 2018, 13, 1066-1071)

Best poster presentation of 2018

Ms Ayaat Mahmoud (Monash) For a comprehensive list of awards, memberships and grant successes, see pages 67-69.

Cellular processing of N-acylated poly(amino ester)s

2018 CBNS Student Travel Awards:

Mr Jiaul Islam Ms Caterina Brighi Ms Hannah Kelly Ms Kristel Tjandra Ms Hayley Schultz (Monash) (UQ) (Melbourne) (UNSW) (UniSA)

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Media Coverage Media coverage of CBNS provides the public with important insights into the diverse range of research being undertaken at the Centre. It highlights its contribution to tackling crucial advances in the understanding and treatment of human disease. This coverage also gives Centre members a key opportunity to contribute to public debates on science and to facilitate increased understanding of the importance of scientific progress. We maintained our strong media presence during 2018, with 118 newspaper or magazine articles, 6 radio interviews, and this year 10 television appearances. A particularly successful story published by multiple outlets was the work being led by CBNS CI and Node Leader Professor Justin Gooding and CBNS CI Professor Maria Kavallaris, both UNSW. Praised as the “Holy Grail of cancer research”, they published a paper on the development of a revolutionary blood test, which can make diagnosing cancer much easier and faster than today’s tests. With this revolutionary method, it will be possible to detect malignancies long before they appear on imaging scans.

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118

newspaper or magazine articles

This work was highlighted both on TV (Channel Nine, Ten and various channels) as well as on ABC Radio (see http://www.abc.net.au/radio/ adelaide/programs/am/australianscientists-develop-blood-test-to-detectcancer-early/10172162) and various newspapers.

radio appearances

television appearances

Publication Highlights Published papers reflect the success of the Centre and give CBNS members the opportunity to expand their network, promote their ideas within the scientific community and refine their research. This year, the Centre published 212 papers with 28 with an impact factor (IF) >10. In this section, we will highlight four especially strong papers published in the journal Nature.

Nucleic acid hybridization on an electrically reconfigurable network of gold-coated magnetic nanoparticles enables microRNA detection in blood As highlighted on page 52, in August, CBNS CIs Professor Maria Kavallaris and Professor Justin Gooding from UNSW published a revolutionary paper on early cancer detection in Nature Nanotechnology. This publication was collaboratively created with CBNS alumna Dr Roya Tavallaie, CBNS members Dr Joshua McCarroll, Dr Marion Le Grand and CBNS alumnus Dr Nicholas Ariotti. The researchers have developed a new blood test that is able to detect concentrations of microRNA from 10 aM to 1nM – long before they appear on imaging scans. In the paper, the team members showed that, through electric-field-induced reconfiguration of a network of gold-coated magnetic nanoparticles modified by probe DNA (DNA–Au@MNPs), it is possible to create a highly sensitive sensor for direct analysis of nucleic acids in samples as complex as whole blood. It can distinguish small variations in microRNA concentrations in blood samples of mice with growing tumours – which makes this device a promising tool for cancer diagnostics. Nature Nanotechnology, 13, 1066-1071

Minimum information reporting in bio-nano experimental literature In September, CBNS members published an internationally discussed paper on proposed guidelines to improve the quality and reproducibility of bio-nano material studies in the journal Nature Nanotechnology. This paper was a picture-perfect example for collaborative work among CBNS members and partners. Mr Matt Faria (Melbourne) was the main author of the paper titled Minimum information reporting in bio-nano experimental literature and it was co-authored by the following CBNS members and alumni: CBNS alumni Dr Mattias Björnmalm, CBNS CI Associate Professor Kris Thurecht, CBNS CI Professor Stephen Kent, CBNS CI Professor Rob Parton, CBNS CI Professor Maria Kavallaris, CBNS CI

Dr Angus Johnston, CBNS CI and Node Leader Professor Justin Gooding, CBNS CI Dr Simon Corrie, CBNS CI Professor Ben Boyd, CBNS CI Professor Pall Thordarson, CBNS CI and Node Leader Professor Andrew Whittaker, CBNS PI Professor Molly Stevens, CBNS CI Professor Clive Prestidge, CBNS CI Professor Chris Porter, 2017 CBNS Visiting Professor Professor Wolfgang Parak, CBNS Director Professor Tom Davis, CBNS CI Professor Edmund Crampin and CBNS Deputy Director Professor Frank Caruso. The paper suggests a ‘minimum information standard’ for experimental literature investigating bio-nano interactions. The authors state that this standard should consist of specific components to be reported and divided into three categories: material characterisation, biological characterisation and details of experimental protocols. The researchers’ intention was for these proposed standards to improve reproducibility, increase quantitative comparisons of bio-nano materials and facilitate meta-analyses and in silico modelling. Nature Nanotechnology, 2018, 13, 777-785

A photoelectrochemical platform for the capture and release of rare single cells International collaborations between UNSW and Imperial College London developed a paper on the capture and release of rare single cells on a photoelectrochemical platform. CBNS alumni Dr Stephen Parker explored the complexities of rare single cells. To control the release of a single cell is a considerable challenge that requires two stimuli so that the cell can first be observed and then released when desired. Further CBNS involvement includes CI and Node Leader Professor Justin Gooding and CI Professor Maria Kavallaris, CBNS postdoctoral researchers Dr Friederike Mansfield, Dr Ying Yang and Ms Kathleen Kimpton. The paper was co-authored by alumna Dr Bakul Gupta, from Imperial College London. Although powerful methods for studying cell heterogeneity have emerged, they

are more suitable for common rather than rare cells. Published in June 2018, the paper details a versatile capture and release platform consisting of an antibody-modified and electrochemically cleavable semiconducting silicon surface for release of individual cells of interest is presented. The captured cells can be interrogated microscopically and tested for drug responsiveness prior to release and recovery. The capture and release strategy was applied to identify rare tumour cells from whole blood, monitor the uptake of, response to and subsequently select cells for singlecell gene expression based on their response. This work was published in Nature Communications in June 2018. Nature Communications, volume 9, Article number: 2288 (2018)

Coatings super-repellent to ultralow surface tension liquids Published in October 2018, PhD student Mr Shuaijun Pan (Melbourne) was the principal author on the Nature Communications paper Coatings superrepellent to ultralow surface tension liquids. Co-authored by CBNS alumni Dr Mattias Björnmalm and Dr Nadja Bertleff-Zieschang, CBNS member Dr Joseph J. Richardson (Melbourne) and Deputy Director Professor Frank Caruso (Melbourne), the paper reported a bottoms-up approach to preparing high-performance coatings where durable and liquid repellent coatings are of significant interest for fundamental and practical applications. A mixture of fluorosilanes and cyanoacrylate was prepared creating super-repellent coatings, that were able to withstand scratch, shear, abrasion, peeling, sonication and washing, exhibiting negligible decreases in performance. On application to surfaces, the coatings assemble into thin films of locally multire-entrant hierarchical structures with very low surface energies. Furthermore, the coatings are transparent, durable and demonstrate universal liquid bouncing, tailored responsiveness and anti-freezing propertie, and are thus a promising alternative to existing synthetic super-repellent coatings. Nature Materials, volume 17, pages 1040–1047 (2018)

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Governance CBNS Annual Report 2018 55

Governance and Management The CBNS Governance Board has an independent advisory role to the Centre Director, Executive and Management Committee. The Board provides guidance on governance, strategy and stakeholder engagement. The Scientific Advisory Board provides strategic scientific insight and commercial direction to the Centre. The CBNS Executive meet regularly to discuss the research program, operational matters and Centre finances.

Governance Board

Scientific Advisory Board

Chaired by Professor Peter Doherty

Chaired by Professor Alan Rowan

CBNS Executive Director, Deputy Director, Manager

Director Professor Tom Davis Deputy Director Professor Frank Caruso

Education and Training Sub-Committee

Outreach Sub-Committee Chaired by Associate Professor Matthew Kearnes

Chaired by Professor Maria Kavallaris

Research Program: Research Themes and Signature Projects

Strategic Fund

Monash node

Melbourne node

UQ node

UNSW node

UniSA node

Professor Tom Davis Node Leader

Professor Frank Caruso Node Leader

Professor Mark Kendall / Professor Andrew Whittaker Node Leader

Professor Justin Gooding Node Leader

Professor Benjamin Thierry Node Leader

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Governance Board The CBNS Governance Board Members are all experts and leaders in research and governance in scientific, research-based organisations. The Board monitors progress towards the delivery of Key Performance Indicators and approves research, operational and financial plans. The Governance Board met twice in 2018: on 26 April and on 23 August.

Professor Peter C. Doherty

A graduate of the University of Queensland School of Veterinary Science, Professor Doherty shared the Nobel Prize in medicine 1996 for his immunology research and was the Australian of the Year in 1997. Since then, he has gone in to bat for evidence-based reality, relating to areas as diverse as childhood vaccination, global hunger and anthropogenic

climate change. So far, he has published 5 “lay” books on science with the latest, “The Knowledge Wars”, suggesting how those who don’t know much (or anything) about science can “interrogate” both the scientific evidence and those who claim to be scientists for themselves.

Professor Calum J. Drummond

Professor Drummond is currently Deputy ViceChancellor Research and Innovation and Vice President at RMIT University, playing a leadership role in the development of discovery and practicebased research and in building and enhancing capability in research and innovation across the University. He is also an active research professor and has published over 200 papers and patents in the area of advanced materials, including

biomedical and energy storage applications. Professor Drummond joined RMIT University in 2014 from CSIRO where he was Group Executive for Manufacturing, Materials and Minerals comprising 1300 researchers and research support staff. Earlier he was seconded from CSIRO to be the inaugural Vice President Research at CAP-XX, an Intel portfolio company that developed supercapacitors for consumer electronic products.

Professor Haber is Executive Director of the Children’s Cancer Institute and Head of the Institute’s Experimental Therapeutics Program. She is internationally recognised for her worldclass research into the treatment of neuroblastoma and acute lymphoblastic leukaemia in children. By identifying molecular targets that drive the growth and development of cancer in children, her project team are working towards more effective treatments for individual childhood cancers as well as through developing new drugs to inhibit the action of these targets. Combining existing and new drug treatments into novel therapeutic approaches that can be rapidly translated into national and international clinical trials. She has received numerous awards for her research, particularly in terms of translating her research findings into the clinic, including the 2014 Cancer Institute NSW Premier’s Award for Outstanding

Cancer Researcher of the Year and in 2015 was appointed an inaugural Fellow of the Australian Academy of Health and Medical Sciences. She was one of three Finalists for the 2017 CSIRO Eureka Prize for Leadership in Innovation and Science. Professor Haber has a long and continuous record of peer-reviewed grant funding and an excellent track record with more than 175 journal publications. She is currently leading the Zero Childhood Cancer initiative. A national child cancer personalised medicine program, which will enable all newly-diagnosed, high-risk childhood cancer patients in Australia, and all children who relapse following treatment, to have their therapy tailored to the specific genetic and biological characteristics of their individual tumour. The national clinical trial for this program was opened in September 2017.

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Professor Michelle Haber

Ms Maureen O’Keefe

Ms O’Keefe was appointed as Chief Executive Officer of the Australian College of Optometry in March 2013, an organisation providing public health eye care, tertiary clinical teaching and education, and research to preserve sight and prevent blindness. She spent the previous seven years as Chief Operating Officer at the Walter and Eliza Hall Institute of Medical Research and prior to that, held several senior executive roles at the University of Melbourne. Ms O’Keefe is a graduate of the Australian Institute of Company Directors, the Williamson Community Leadership Program and an Executive Education Program at Massachusetts Institute of Technology, Cambridge.

Ms O’Keefe has spent her career in higher education, research and health organisations and has more than fifteen years’ experience in senior executive roles. She is a Board member of Vision2020 Australia and the BioMelbourne Network. Previously, Ms O’Keefe was a member of the Council of the Victorian Cancer Agency for 6 years, a Ministerial appointment, including two years as a member of the VCA Clinical Trials Working Group and more recently a Ministerial appointment on the Victorian Department of Health’s Clinical Trial Research Consultative Council.

Dr Warwick Tong

Dr Tong is currently the CEO of the Cancer Therapeutics Cooperative Research Centre (CRC). In 2013, he led the CRC through a successful extension application receiving another six years of funding until 2020. Formerly a director of Primecare Medical Ltd (NZ) and MedInnovate Ltd (UK) he is currently a member of the Advisory Board for Cortex Health. He has spent more than 20 years in executive management in drug development and commercial roles in both the major pharmaceutical and biotech industry.

After graduating as Senior Scholar in Medicine from Auckland University and working in General Practice Dr Tong joined Glaxo in NZ as Medical Director and subsequently worked in Singapore and London, in regional and global business development and commercial roles for Glaxo. Prior to coming to Melbourne, Warwick spent five years in Boston as SVP, Development, for Surface Logix Inc.

Professor Gordon Wallace (AO)

Professor Wallace is currently the Executive Research Director of the ARC Centre of Excellence for Electromaterials Science (ACES) and the Director of the Australian National Fabrication Facility, Materials Node. Professor Wallace is a Fellow of the Australian Academy of Science, the Australian Academy of Technological Sciences and Engineering (ATSE), the Institute of Physics, the Royal Australian Chemical Institute (RACI) and the Royal Society of NSW. He is a corresponding member of the Academy of Science in Bologna. On 26 January 2017, he was appointed as an Officer of the Order of Australia. Furthermore, he received Wollongong’s award for Innovation in 2017, served as Wollongong’s Australia Day Ambassador and was named NSW Scientist of the Year 2017. He received the Eureka Prize for Leadership in Science and Innovation in 2016. In 2015, he was appointed to the Prime Ministers Knowledge Nation.

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Professor Wallace is involved in the design and discovery of new materials for use in Energy and Health. In the health area, this involves using new materials to develop biocommunications from the molecular to skeletal domains in order to improve human performance. In the energy area, this involves the use of new materials to transform and to store energy, including novel wearable and implantable energy systems for the use in medical technologies. In order to facilitate the creation of functional devices from fundamental discoveries he has pioneered the development of 3D additive fabrication (including 3D printing) using advanced materials. He is committed to fundamental research and the translation of fundamental discoveries into practical applications. He is a passionate communicator, dedicated to explaining scientific advances to all in the community from the layperson to the specialist.

Scientific Advisory Board The Scientific Advisory Board (SAB) has an independent mentoring and advisory role to the Executive, providing strategic insight and commercial direction to the Centre. The SAB plays a key role in advising the Centre on the development of the strategic and commercialisation plans. The Board advises on patent versus publication strategy, and commercialisation and spin-out strategy as required. They assist in identifying intellectual property, partnering and licensing opportunities and organisations with which CBNS can ally. In addition to the core members of the SAB, there are temporary international members, including the CBNS Visiting Professor, who provide variation to the scientific mix of the SAB.

Professor Alan Rowan

Professor Rowan has performed his research at the interface of chemistry and biology with seminal and pioneering work on processive catalysis and functional self-assembly. His latest scientific achievement has been the development of the first truly biomimetic hydrogel which mimics the mechanic and functional properties of the extracellular membrane.

from magnetic materials, single enzyme catalysis, supramolecular catalysis through to nanometresized solar cells and photonic materials. In the last ten years, he developed the concept of processive catalysis, mimicking the natural exo- and endonucleases and demonstrated that a macrocyclic catalyst can thread onto and move along a polymer substrate in a highly efficient process.

This scientific breakthrough is now being developed commercially for wound dressing, drug therapeutic and cell growth. This has further established Professor Rowan as a truly innovative scientist, working toward understanding at the molecular level the functionality of hierarchical materials and catalysis.

In January 2016 Professor Rowan joined the University of Queensland as the Director of the Australian Institute of Bioengineering and Nanotechnology. He previously led a research group at Radboud University’s Institute of Molecules and Materials, one of Europe’s leading research centres for the nanoscience’s. He was awarded an ARC Australian Laureate Fellowship to continue his work on novel biomimetic and dynamic materials.

Professor Rowan’s considerable scientific curiosities has resulted in him working in many areas, ranging

Dr David Owen

Dr Owen has driven the drug delivery programs by developing and executing a number of successful proof-of-concept studies. The results from these studies have led to a number of commercial partnerships such as Stiefel, a GSK company, Lilly and AstraZeneca, as well as driving Starpharma’s own internal drug delivery program focused on an improved dendrimer-docetaxel formulation.

Dr Julio Ribeiro

Dr Ribeiro completed his PhD in Medicine at UNSW in 1998. He then moved from academia to start a business career at Sigma-Aldrich Pty. Ltd., Australia and NZ, where he had many executive roles, including technical support manager, sales manager and manager of a fine chemical division (SAFC) in Australia and NZ. In 2006, Dr Ribeiro founded Inventia Pty Ltd, a fine chemical and biotechnology company. As Managing Director, Dr Ribeiro oversees R&D projects from conceptualisation to delivery. This includes the conception of projects, contracting scientists, daily operations and writing final reports based on

scientists’ results. In its short period of existence, Inventia has landed several R&D contracts. In 2012, Dr Ribeiro created Inventia Genetic Technologies Pty Ltd (IGT). A subsidiary of Inventia Pty Ltd. IGT is now Australian leading provider of bovine IVF for the beef farmers in QLD. IGT has been conducting several research projects in the field of bovine IVF with the objective of increasing the pregnancy, facilitate oocytes and embryo transport and development of new bovine IVF media. In 2012, in partnership with UNSW, Dr Ribeiro started the 3D Bioprinting project; commercialising the 3D Bioprinting technology. This resulted in the creation of Inventia Life Science Pty Ltd in 2013.

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Dr Owen is the Vice President of Research at Starpharma and has extensive experience in medicinal chemistry and biochemistry, and in managing teams focused on commercially directed drug discovery. He has held several positions in the biotech industry including Mimotopes, Cerylid and Glykoz and has gathered extensive international experience in biotechnology and pharmaceutical research and development. Since joining Starpharma

Professor Fariba Dehghani

Professor Dehghani is the Director of ARC Food Processing Training Centre and the Director of the Centre for Excellence for Advanced Food Enginomics at the University of Sydney, an Associate member of IChemE and a member of ARC College of Experts. She has been selected by Engineers Australia as one of the top 50 Engineers for innovation in research in 2016. She is a reputed researcher who has been instrumental in building an interdisciplinary research environment that stimulates the interface and intersects of engineering, science, business, medicine, pharmacy, and agriculture. Her international team of researchers and industry partners have worked together to provide pragmatic, cost-effective and environmentally sustainable solutions to a diverse range of issues, with the aim of improving lives. Professor Dehghani’s research has made significant contributions to the design of advanced and environmentally friendly techniques for processing materials, the extraction of high value and active

compounds from natural resources for the treatment and prevention of chronic diseases such as infection and cardiovascular disease, and the synthesis of materials for musculoskeletal tissue repairs. Her research is highly regarded, not only in academia but by industry. She has established long-term collaborations with the food and biotech private sectors and acquired substantive funding from national and international competitive grant applications. She has published more than 250 papers and is co-inventor on 13 patents, including nine with potential end users. She has mentored more than 45 postgraduates, more than 100 honours/masters researchers and several successful entrepreneurs. Her research led to the establishment of a start-up company, Trimph Pty Ltd in 2015 by receiving more than $2M fund from a private investor and from government. The product from this company has recently received approval from Therapeutic Goods Administration (TGA) for clinical application.

Professor Jagat R. Kanwar

Professor Kanwar is the Head and Team Leader of Nanomedicine-Laboratory of Immunology and Molecular Biomedical Research (NLIMBR), School of Medicine, Faculty of Health, Deakin University, Australia. Professor Kanwar has an international reputation and expertise in investigating fundamental and applied molecular signalling aspects of the pathogenesis of cancer, chronic inflammation and neurodegenerative diseases, thereby, leading to the development of treatment strategies from bench to bedside. He has more than 150 research publications in high impact factor and peer-reviewed international journals, 27 book chapters and 3 edited books. Professor Kanwar’s research has generated several patents/Patent Cooperation Treaties (PCT) with more than five licensed patents for commercialisation to biopharma industry. His group is currently working on drug discovery and nanomedicine for oral and systemic drug delivery of a range of natural bioactive and biomacromolecules (proteins/peptides, siRNAs and aptamers) for targeting survivin, HIF-1α and other apoptotic and inflammatory cell signalling molecules in cancer, chronic inflammation and neurodegenerative disorders.

His research combines immunology with state of the art and cutting-edge techniques in molecular biology, biochemistry, bio-nano technology and visualisation to investigate the pathways in which key molecules are regulated in both normal and disease states. A number of in vitro human cell/ tissue-based co-culture models for cancers, microbial infections, autoimmune diseases, chronic inflammatory diseases (osteoarthritis, inflammatory bowel disease), gut health, neurodegeneration and immunomodulation have been developed by his group. Professor Kanwar’s main research objective is to understand and target the mechanisms involved at the molecular and subcellular level which gives an edge over the prevalent targeting techniques. He carries out both academic and commercial research projects and develops new approaches for the diagnosis, treatment, and nanomedicine based new generation delivery systems. His recent research focus is on locked nucleic acid (LNA) LNA-modified aptamers conjugated “double targeted nano-bullet nanocapsules” with natural anti-tumour proteins which specifically target cancer cells.

2018 temporary members Professor Warren Chan

Professor Chan is a Distinguished Professor at the Institute of Biomaterials and Biomedical Engineering at the University of Toronto. Dr Chan received his B.S. degree from the University of Illinois in 1996, PhD degree from Indiana University in 2001, and post-doctoral training at the University of California (San Diego). He moved to Toronto in 2002 to lead the Integrated Nanotechnology/Biomedical Sciences Laboratory. His research interest is in the development and translation of nanotechnology for diagnosing and treating cancer and infectious diseases. He has received NSERC E.W.R. Memorial

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Steacie Fellowship, Kabiller Young Investigator Award in Nanomedicine, the BF Goodrich Young Inventors Award, Lord Rank Prize Fund award in Optoelectronics (England), and Dennis Gabor Award (Hungary). He is currently an Associate Editor of ACS Nano. Finally, he is also affiliated with a number of different departments at the University of Toronto: the Department of Materials Science and Engineering, the Terrence Donnelly Centre for Cellular and Biomolecular Research Chemistry, Chemistry and Chemical Engineering.

Professor Maria Kavallaris

CBNS CI Professor Maria Kavallaris is head of the Tumour Biology and Targeting Program at Children’s Cancer Institute, Lowy Cancer Research Centre and co-Director of the Australian Centre for Nanomedicine. Professor Kavallaris’ research focuses on identifying the mechanisms of action and resistance to anticancer drugs, discovering new protein interactions in cancer and the development of less toxic cancer therapies using nanotechnology. Professor Kavallaris has held numerous competitive fellowships. She is a National Health and Medical Research Council (NHMRC) Principal Research Fellow and Fellow of the Australian Academy

of Health and Medical Sciences (AAHMS). Her research contributions have been recognised by international and national awards and prizes including an International Agency for Research on Cancer Fellowship, an American Association for Cancer Research Women in Cancer Research Award, NHMRC Career Development Award, a Young Tall Poppy Award and an Australian Museum Eureka Prize. Professor Kavallaris was the recipient of a 2017 NSW Premier’s Science and Engineering Prize and in 2015 was named an AFR/Westpac 100 Woman of Influence and one of the Knowledge Nation 100.

Scientia Professor Martina Stenzel

Scientia Professor Stenzel is Co-Director of the Centre for Advanced Macromolecular Design (CAMD), School of Chemistry at UNSW. Scientia Professor Stenzel is interested in the design of polymers for biomedical applications, mainly drug delivery. Her research ranges from fundamental aspects of polymerisation synthesis to the application of polymers in medicine. She combines polymers with sugar, proteins and drug moleculars to create smart nanoparticles for drug delivery.

Scientia Professor Stenzel was awarded an ARC Future Fellowship in 2009 and was promoted to Full Professor in 2012. In 2013, she was appointed Co-Director of CAMD. She also holds an AdjunctProfessorship in Medicine at the University of Western Sydney. In 2014, she joined the School of Chemistry at UNSW to establish a research program on polymeric nano- and biomaterials.

Professor Taeghwan Hyeon

2011, he was selected as “Top 100 Chemists” of the decade by UNESCO & IUPAC. He is a Fellow of the Royal Society of Chemistry (RSC) and the Materials Research Society (MRS). He received multiple awards including the Korean S&T Award from the Korean President (2016), Hoam Prize (2012, Samsung Hoam Foundation), POSCO-T. J. Park Award (2008), the IUVSTA Prize for Technology (International Union for Vacuum Science, Technique and Applications, 2016). Since 2010, he has served as an Associate Editor of J. Am. Chem. Soc.

Professor Anja Boisen

Professor Boisen is the Head of the Centre of Excellence at the Department of Micro and Nanotechnology at the Technical University of Denmark. Professor Boisen has a thorough knowledge of micromechanics and nanotechnology. Her research group focuses on the development and application of micro and nanosensors as well as microsystems for oral drug delivery and has spun out several companies including Cantion, Silmeco, and BluSense Diagnostics.

Professor Boisen is a member of the Board of the Danish Innovation Foundation, the Board of the Villum Foundation, the Danish Academy of the Technical Sciences and the Royal Danish Academy of Sciences. In 2008, she was awarded the largest research prize in Denmark, the Villum Kann Rasmussen award, followed by the EliteForsk Award from the Danish Ministry of Research, Innovation and Higher Education in 2012. Anja also received the ‘Sapere Aude – top researcher award’ from the Danish Council for Independent Research in 2013.

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Professor Hyeon is a Seoul National University (SNU) Distinguished Professor and a Director of the Centre for Nanoparticle Research – Institute for Basic Science (IBS). Since he joined the faculty of the School of Chemical and Biological Engineering of Seoul National University in September 1997, he has been focused on the synthesis and applications of uniform-sized nanoparticles and related nanostructured materials and has published over 350 papers in prominent international journals (> 41,000 citations and h-index of > 110). In

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Performance

Performance and KPIs CBNS achievements are evaluated by the Australian Research Council on an annual basis. CBNS performance is assessed against key performance indicators (KPIs) that were set at the commencement of the Centre and have been reviewed in 2017. These cover the traditional research metrics of outputs like journal publications and conference presentations. There are also metrics that cover the interdisciplinarity of CBNS research, new research partnerships, measures of esteem, public awareness and student and ECR mentoring. CBNS is progressing well. The KPIs for 2018 are summarised in this infographic. It shows the targets for each of the KPI areas as well as our actual achievements.

Research findings: Number of research outputs: Journal publications target: 200

Patents (filed) target: 3

actual: 212

actual: 11

Book chapters [book] target: 7 actual: 5

Quality of research outputs: Publications impact factor >10 target: 16 actual: 28 % journals articles impact factor >2 target: 70% actual: 92%

New research fellowships target: 3

Membership on editorial boards target: 20

actual: 21

actual: 65

Number and nature of commentaries about the Centreâ&#x20AC;&#x2122;s achievements Articles target: 40

Media releases target: 15

Number of invited talks/papers/ keynote lectures given at major international meetings target: 50

actual: 118

actual: 18

actual: 74

Collaboration and interdisciplinarity:

Research outputs featuring co-authorship between nodes target: 18

Postgraduates/ postdocs working on cross node or interdisciplinary projects target: 12

actual: 37

actual: 233

Number of research outputs co-authored with alumni target: 6

actual: 37

Number of new organisations collaborating with, or involved in, the Centre, target: 15 actual: 137

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Number of alumni participating in Centre research program (e.g. mentor, invited speaker, student supervisor, lab visit-exchange, project collaborator) target: 5

Number of postgrads or postdocs that have moved (i.e. employment) between collaborating organisations and partner organisations to work on research projects target: 4

actual: 32

actual: 17

Research training and professional education: Number of professional training courses for staff and postgraduate students attended target: 15

Number of Centre attendees at all training/development courses offered by the Centre target: 75

actual: 60

actual: 179

Number of ECRs (within five years of completing PhD) working on core CBNS research target: 20

Number of new postgrads working on core CBNS research target: 15

actual: 64

actual: 32

Number of students mentored target: 80

actual: 193 Number of mentoring programs offered by the Centre, target: 7 actual: 11 Number of new postdocs recruited to CBNS working on core CBNS research target: 10

actual: 27

Number of new Honours students working on core CBNS research target: 12

actual: 16

Number of completions by PhD students target: 15

actual: 35 Number of completions by Masters students target: 2 actual: 2 Number of completions by Honours students target: 8 actual: 15

Gender equity and diversity:

Number of Centre-led initiatives that promote gender equity and diversity and a familyfriendly workplace target: 3

% meetings and workshops facilitated by the Centre that enable attendance by members with dependents target: 50%

% Centre-organised meetings with gender balance amongst organising committees, speakers and participants target: 60%

actual: 11

actual: 50%

actual: 62.5%

% of women in mentoring program target: 50%

Number of ECRS and students holding leadership roles within the Centre target: 25

actual: 55%

actual: 50

International, national and regional links and networks: Examples of relevant interdisciplinary research supported by the Centre:

actual: 81

Number of government, industry and business community briefings target: 10

actual: 25

Number of national and international workshops held/ organised by the Centre, target: 6

Number of visits to overseas laboratories and facilities target: 30

actual: 9

actual: 57

Number of website hits (page views) target: 40,000

Number and nature of public awareness/ outreach programs target: 12

actual: 54,466

actual: 32

Research outputs that result from CoE interdisciplinary research target: 10

actual: 159

Number of talks given by Centre staff open to the public, target: 10

actual: 15

Number of interdisciplinary projects in the research program target: 12

actual: 139

Video material produced and publicised each year on the website target: >20mins

actual: 28mins

CBNS Annual Report 2018 65

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Number of international visitors and visiting fellows target: 40

Financial Report 2018 Income and Expenditure

In Kind contributions

Income

2018 ($)

Institution

2018 ($)

ARC Grant Income

4,244,123

Monash University

1,483,725

Collaborating organisation contribution

1,506,313

University of Melbourne

1,270,633

Other income (including interest distribution)

11,577

Total income

5,762,013

Expenditure

2018 ($)

University of New South Wales

740,000

University of Queensland

652,313

University of South Australia

1,151,456

Salaries 4,409,816

Australian Synchrotron

1,114,656

Equipment 119,320

Australian Nuclear Science and Technology Organisation 16,930

Consumables and maintenance

992,072

Travela 539,375 Scholarships and Student support

435,103

Administration 115,910 Otherb 278,387 Total expenditure

Carry forward

6,889,984

4,955,498

Sungkyunkwan University

45,000

University of Wisconsin-Madison

15,000

University of Warwick

64,000

University of Nottingham

31,961

Imperial College London

5,000

Memorial Sloan-Kettering Cancer Center University College Dublin

65,000 5,000

Carry forward adjustmentc 1,564,218

University of California, Santa Barbara

26,000

Balance 2,263,310

Columbia University Medical Center

69,810

a Includes accommodation and conference expenses b Includes grant donation and payment, finance related cost, telecommunication, hospitality c Adjustment made in annual report for 2017 carry forward. Opening balance reported was $4.9M but the actual balance was $3.3M. The difference is due to the cash contribution statements from nodes which was not reported in previous years.

Cash contributions Institution

2018 ($)

Monash University

375,729

University of Melbourne

335,018

University of New South Wales

308,333

University of Queensland

305,911

University of South Australia

181,322

Total 1,506,313

66 CBNS Annual Report 2018

Total 6,756,484

Awards, Memberships and Grant Success

Fellow of the Australian Academy of Technological Science and Engineering CBNS CI and Node Leader Professor Justin Gooding (UNSW) was elected a Fellow of the Australian Academy of Technological Science and Engineering (ATSE). The Academy elected Professor Gooding for his more than 20 years of pioneering and sustained contributions to the field of biosensor research. Fellow of the Australian Academy of Health and Medical Sciences Professor Stephen Kent (Melbourne) has been elected a Fellow of the Australian Academy of Health and Medical Sciences in recognition of his authoritative expertise and exceptional contribution to the health and medical research fields as well as his continuing involvement in issues of healthcare, prevention of disease, education, research, health services policy and delivery. NHMRC Project Grant Professor Benjamin Thierry (UniSA) has been awarded a NHMRC Project Grant to use immuno-PET imaging to detect metastatic deposits in regional lymph nodes in head and neck cancer, sparing patients unnecessary surgery and improving treatment. The project is a collaboration between the Future Industries Institute, the Royal Adelaide Hospital and the University of Stanford. ARC Linkage Project CBNS CIs Professor Justin Gooding and Professor Maria Kavallaris (both UNSW) won an ARC Linkage Project grant for their project Bioinks for the 3D printing of cells made from off-the-shelf components. This project was a collaboration with Scientific Advisory Board member Dr Julio Ribeiro from Inventia Life Science Pty Ltd.

NHMRC Early Career Fellowship Dr Cheng Zhang (UQ) from CBNS CI Professor Andrew Whittaker‘s group was awarded an NHMRC Early Career Fellowship for his project Translatable Polymeric Nanomedicines towards Clinical Use. His project aims to develop an effective synthetic method with excellent reproducibility to produce translatable and preciselydefined polymeric nanomedicines and to investigate the factors affecting their therapeutic efficacy. During the four years of the fellowship, Cheng will spend two years at the University of California, Santa Barbara with CBNS PI Professor Craig Hawker and another two years at UQ with CBNS CI Professor Andrew Whittaker. NHMRC Senior Principal Research Fellowship CBNS CI Professor Rob Parton (UQ) has been awarded a NHMRC Senior Principal Research Fellowship for his project Structural and Functional Analysis of Plasma Membrane Microdomains in Health and Disease. Within the next four years, Professor Parton will work on examining how lipids and proteins work together to make speciliased regions and aims to understand what goes wrong in diseases such as muscular dystrophy. Leslie (Les) J. Fleming Churchill Fellowship CBNS CI Dr Simon Corrie (Monash) has been awarded the Leslie (Les) J. Fleming Churchill Fellowship. He will undertake expert training in pre-clinical evaluation of implantable sensors for cancer diagnostics in the USA. The Churchill Trust was established in 1965 to honour the memory of Sir Winston Churchill and fulfil his wish for people from all walks of life to travel the world to gain new knowledge and share ideas and insights. Fellowships are awarded to Australians who are passionate about challenging the status quo to create or make a positive impact on our society.

Fellow of the Royal Society of London CBNS CI and Deputy Director Professor Frank Caruso (Melbourne) has been elected a fellow of the Royal Society of London. The Fellowship of the Royal Society are the most eminent scientists, engineers and technologists from or living and working in the UK and the Commonwealth. Each year up to 52 Fellows and up to 10 Foreign Members are elected from a group of about 700 candidates.

Awards Professor Ben Boyd (Monash) Australian Friends of Tel Aviv University – Monash University (AFTAM) Award Faculty Research Impact (Social and Economic) Award Professor Frank Caruso (Melbourne) Clarivate Analytics Highly Cited Researcher The Batteard-Jordan Australian Polymer Medal Department of Defense Hearing Restoration Research Program Translational Research Award, “Repairing Cochlear Damage with Neurotrophin Therapy” Dr Simona Carbone (Monash) Brian Smith Memorial Award Young Investigator Prize from the European Society of Neurogastroenterology and Motility Professor Tom Davis (Monash) Australian Friends of Tel Aviv University – Monash University (AFTAM) Award Clarivate Analytics Highly Cited Researcher Dr Nicholas Fletcher (UQ) CASS ECR Travel Award for the 19th International Conference on Nanotechnology and Nanomedicine (ICNN) CAI Postdoc Travel Award to attend ICNN ICNN Best Session Presenter Professor Justin Gooding (UNSW) Fellow of the Australian Academy of Technology and Engineering (ATSE)

CBNS Annual Report 2018 67

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Highlights

Associate Professor Matthew Kearnes (UNSW) European Association for the Study of Science and Technology (EASST) book award Professor Stephen Kent (Melbourne) Fellow of the Australian Academy of Health and Medical Sciences Dr Adam Martin (UNSW) Young Investigator Highlights Award Professor Chris Porter (Monash) FarmDhc farmacie hedersdoktor, Uppsala University, Sweden (honorary doctorate) Clarivate Analytics Highly Cited Researcher Dr Joseph J. Richardson (Melbourne) Best Presentation at the Frontiers in Bio-Nano Science (FBNS 2018) Dr Katharina Richter (UniSA) ASMR Ross Wishart Memorial Award Dr Kristel Tjandra (UNSW) ATA Scientific Encouragement Award Dr Marnie Winter (UniSA) Best Oral Presentation at the Frontiers in Bio-Nano Science (FBNS 2018) Dr Yanfang Wu (UNSW) UNSW Science PhD Writing Scholarship for Oral Presentation at the International Conference on Nanoscience and Nanotechnology Outstanding Reviewer, Poster presentation at Electrochemistry at Nano- interfaces: Faraday Discussion

Elected Fellows Professor Ben Boyd (Monash) College of Fellows from the Controlled Release Society (CRS) Professor Frank Caruso (Melbourne) Fellow of the Royal Society of London Professor Justin Gooding (UNSW) Fellow of the Australian Academy of Technological Science and Engineering (ATSE)

Journal of Liposome Research Journal of Pharmaceutical Sciences Journal of Pharmacy and Pharmacology Dr Simona Carbone (Monash) American Journal of PhysiologyGastrointestinal and Liver Physiology Professor Frank Caruso (Melbourne) ACS Applied Bio Materials ACS Central Science ACS Nano Advanced Functional Materials Advanced Science Advances in Colloid and Interface Science Angewandte Chemie Biomacromolecules Biomaterials Science CCS Chemistry Chemistry of Materials Nano Today Professor Edmund Crampin (Melbourne) Bulletin of Mathematical Biology Current Opinion in Systems Biology npj Systems Biology and Applications Professor Tom Davis (Monash) Editorial Advisory Board (EAB) ACS Macro Letters Professor Justin Gooding (UNSW) Editor-in-Chief, ACS Sensors Royal Society of Chemistry journal, Chemical Sciences ACS journal, Bioconjugate Chemistry Member of the editorial board (Handling) of the American Scientific Publishers Journal of Chemical and Biological Interfaces Elsevier journal Electrochemistry Communications Elsevier journal Sensors and Actuators B Humana Press journal Nanobiotechnology MDPI journal Biosensors MDPI journal Nanomaterials Wiley journal Electroanalysis Wiley-VCH journal ChemElectroChem Wiley-VCH journal Chemistry – A European Journal

Professor Stephen Kent (Melbourne) Fellow of the Australian Academy of Health and Medical Sciences

Professor Maria Kavallaris (UNSW) American Journal of Cancer Research Nanomedicine-Nanotechnology, Biology and Medicine

Member of Editorial Boards

Associate Professor Matthew Kearnes (UNSW) Science as Culture Science Technology and Society

Professor Ben Boyd (Monash) Current Drug Delivery Drug Delivery and Translational Research Journal of Colloid and Interface Science

68 CBNS Annual Report 2018

Professor Stephen Kent (Melbourne) Frontiers in Immunology

Journal of Virology Sexual Health The Journal of Immunolgy Vaccine Professor Robert Parton (UQ) Molecular Biology of the Cell Traffic Dr Daniel Poole (Monash) American Journal of PhysiologyGastrointestinal and Liver Physiology Professor Chris Porter (Monash) Journal of Pharmaceutical Sciences Journal of Pharmacy and Pharmacology Molecular Pharmaceutics Pharmaceutical Research Professor Clive Prestidge (UniSA) AAPS Open Journal of Liposome Research Mesoporous Biomaterials Professor Benjamin Thierry (UniSA) Plos One Scientific Reports Professor Pall Thordarson (UNSW) Australian General Chemistry ChemSystemsChem Associate Professor Kris Thurecht (UQ) Nanoscience and Nanotechnology Dr Nicholas Veldhuis (Monash) American Journal of PhysiologyGastrointestinal and Liver Physiology Professor Andrew Whittaker (UQ) Chinese Journal of Polymer Science Journal of Applied Polymer Science Radiation Physics and Chemistry Dr Cheng Zhang (UQ) Journal of Nanoscience and Nanomedicine

Committee and Other Memberships Professor Frank Caruso (Melbourne) Distinguished Academic Awards Committee – The University of Melbourne Lloyd Rees Lecture Awards Committee – Australian Academy of Science Research Advisory Committee, Institute for Future Environments – Queensland Institute of Technology Scientific Advisory Board, LEO Center for Cutaneous Drug Delivery – University of Copenhagen Working Party on Australia-Germany Polymer Interactions – AustraliaGermany Advisory Group

Dr Yi Ju (Melbourne) Chinese Association of Professionals and Scholars, Australia

Grant Success During 2018, additional funding was secured to further support research being undertaken at the Centre. This funding reinforces the collaborative nature and expertise of CBNS researchers. Details of grant successes are as follows:

ARC grants The following CBNS researchers secured additional funding through ARC grants: Discovery Outcome, Linkage, Infrastructure Equipment and Facilities (LIEF) Outcome and Linkage Project: Professor Benjamin Thierry (UniSA) Professor Pall Thordarson (UNSW) Professor Justin Gooding (UNSW) Professor Maria Kavallaris (UNSW)

Australian Competitive Grants Register (ACGR) grants The following CBNS researchers secured additional funding through National Health Research Medical Research Council (NHRMC) grants: Development, Project and Funding Outcome: Professor Frank Caruso (Melbourne) Professor Justin Gooding (UNSW) Dr Kristian Kempe (Monash) Professor Benjamin Thierry (UniSA) Dr Adam Wheatley (Melbourne) Dr Nicky Thomas (UniSA) Professor Clive Prestidge (UniSA)

Public sector grants Dr Nicky Thomas (UniSA) Professor Clive Prestidge (UniSA)

Other grants Dr Alessia Amodo (Melbourne) Dr Changkai Fu (UQ) Dr Kristian Kempe (Monash) Ms Anna Kilsuric (UniSA) Dr Giri Krishnan (UNSW) Dr Tuan Nguyen (UQ) Dr Laura Selby (Monash)

National Health and Medical Research Council (NHMRC) Fellowships Professor Frank Caruso (Melbourne) NHMRC Senior Principal Research Fellowship – Advancing Nanomedicine through Particle Technology

Professor Stephen Kent (Melbourne) NHMRC Senior Principal Research Fellowship – Improved HIV and influenza vaccines Professor Rob Parton (UQ) NHMRC Senior Principal Research Fellowship – Structural and Functional Analysis of Plasma Membrane Microdomains in Health and Disease Dr Katharina Richter (UniSA) NHMRC CJ Martin Biomedical Early Career Fellowship – Maximising the effectiveness of antimicrobial treatments for infection control after surgery Dr Cheng Zhan (UQ) NHMRC Early Career Fellowship – Translatable Polymeric Nanomedicines towards Clinical Use

ARC Fellowships Dr Michele Bastiani (UQ) ARC Discovery Career Researcher Career Award

Fellowships Dr Simon Corrie (Monash) Les Jay Fleming Churchill Fellowship

Student Awards Ms Dewan Akhter (UQ) Best Poster Presentation at the Frontiers in Bio-Nano Science (FBNS 2018) Ms Tahnee Dening (UniSA) 3 Minute Thesis Competition Ms Helen Forgham (UNSW) PhD top up award from Kids Cancer Alliance Mr James Humphries (UQ) UQ Rowe Scientific Chemistry Honours Bursary Mr Jiaul Islam (Monash) Graduate Research International Travel Award

Tow Coast Association Research Awards Day 2018 award for Health and Medical Research Early Career Mr Shuaijun Pan (Melbourne) Eugen Singer Award Ms Emily Pilkington (Monash) Vaughan Scholarship Mr Kye Robinson (Monash) ANN Nanomed Travel Bursary 2018 for the 9th International Nanomedicine Conference AVS 65th International Symposium and Exhibition Dorothy M. and Earl S. Hoffman Travel Grant 2018 Ms Hayley Schultz (UniSA) UniSA Research Degree Excellence Grant Local Chapter Young Scientist Travel Award for the Controlled Release Society Annual Meeting Ms Santhni Subramaniam (UniSA) 1st in Poster to Podium competition at the Controlled Release Asia Meeting Ms Xiaohan Sun (Monash) Best Poster Prize at the Controlled Release Asia Meeting Ms Estelle Suys (Monash) 2nd Place Oral Presentation (Best Podium Award) at the Globalization of Pharmaceutics Education Network Conference (GPEN) Ms Chelsea Thorn (UniSA) Local Chapter Young Scientist Travel Award for the Controlled Release Society Annual Meeting 3rd in Poster to Podium competitation at the Controlled Release Asia Meeting Ms Yunxin (Cindy) Xiao (Monash) Postgraduate Research Award presented at the Australian Institute of Nuclear Science and Engineering

Post-graduation Publication Award (PPA)

ACS Applied Nano Materials Poster Prize received at the 9th International Nanomedicine Conference

Best Oral Presentation by ECR for presentation at the BioProcessing Network Conference

Australian Nanotechnology Network Travel Grant for the 9th International Nanomedicine Conference

Mr John Kokkinos (UNSW) Outstanding Achievement in Research Award from the Australian Society for Medical Research NSW Scientific Meeting 2018

Mr Ken Young (Monash) People’s Choice Award for Poster Presentation at Frontiers in Bio-Nano Science (FBNS 2018)

American Pancreatic Association Annual Meeting 2018 award for Young Investigator

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Ms Linda Hong (Monash) Young Scientist Committee for the Controlled Release Society

Bioscience Reports Award for Best Poster from the Avner Pancreatic Cancer Symposium 2018

CBNS Annual Report 2018 69

CBNS Personnel Chief Investigators Professor Tom Davis ARC Australian Laureate Fellow, CBNS Director and Node Leader, Delivery Systems, Imaging Technologies Monash University Professor Frank Caruso ARC Australian Laureate Fellow, CBNS Deputy Director and Node Leader, Delivery Systems, Imaging Technologies, Vaccines University of Melbourne Professor Justin Gooding CBNS Node Leader, Sensors and Diagnostics Theme Leader University of New South Wales Professor Stephen Kent Delivery Systems and Vaccines Theme Leader University of Melbourne Professor Christopher Porter Delivery Systems and Vaccines Theme Leader Monash University Professor Andrew Whittaker CBNS Node Leader, Effective from 19 March 2018, Imaging and Visualisation Theme Leader, ARC Australian Professorial Fellow, Imaging Technologies University of Queensland

70 CBNS Annual Report 2018

Professor Edmund Crampin Systems Biology and Computational Modelling, Delivery Systems University of Melbourne

Professor Pall Thordarson ARC Future Fellow, Delivery Systems, Imaging Technologies University of New South Wales

Associate Professor Matthew Kearnes ARC Future Fellow, Social Dimensions of Bio-Nano Science and Technology University of New South Wales

Associate Professor Kris Thurecht ARC Future Fellow, Delivery Systems, Imaging Technologies, Vaccines University of Queensland

Professor Mark Kendall CBNS Node Leader, Vaccines University of Queensland Effective until 19 March 2018 Dr Angus Johnston ARC Future Fellow, Delivery Systems, Vaccines Monash University

Professor Benjamin Boyd ARC Future Fellow, Delivery Systems, Sensors and Diagnostics, Vaccines Monash University Professor Maria Kavallaris Delivery Systems, Sensors and Diagnostics University of New South Wales Professor Rob Parton Delivery Systems, Vaccines University of Queensland

Dr Simon Corrie ARC DECRA Fellow, Sensors and Diagnostics University of Queensland

Associate Professor John McGhee Visualisation University of New South Wales

Professor Clive Prestidge Nanomedicine, Drug Formulation and Delivery University of South Australia Professor Benjamin Thierry CBNS Node Leader, Biodiagnostic Technologies University of South Australia

Partner Investigators

Professor Cameron Alexander Head of Division of Drug Delivery and Tissue Engineering, Faculty of Science University of Nottingham, UK Professor Nigel Bunnett Gerald and Janet Carrus Professor of Surgical Sciences (in Surgery and Pharmacology), Columbia University Medical Center, USA

Professor Kenneth Dawson Director of the Centre for BioNano Interactions, Chair of Physical Chemistry University College Dublin, Ireland

Professor Doo Sung Lee Director Theranostic Macromolecules Research Center, Dean of College of Engineering Sungkyunkwan University, South Korea

Dr Ivan Greguric Head of Radiochemistry Australian Nuclear Science and Technology Organisation, Australia

Professor Jason Lewis Vice Chair for Research, Chief of the Radiochemistry and Imaging Sciences Service Memorial Sloan Kettering Cancer Center, USA

Professor David Haddleton Head of Inorganic and Materials Section, Department of Chemistry University of Warwick, UK

Professor Craig Hawker Director of the California Nanosystems Institute, Dow Materials Institute, Co-Director of the Materials Research Lab University of California, Santa Barbara, USA

Professor Molly Stevens Research Director for Biomedical Material Sciences, Institute of Biomedical Engineering Imperial College London, UK

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Professor Nicholas Abbott John T. and Magdalen L. Sobota Professor, Hilldale Professor, and Director, Materials Research and Engineering Center University of Wisconsin-Madison, USA

CBNS Annual Report 2018 71

Research staff Postdoctoral and other research staff Dr Nicolas Alcaraz Postdoctoral Researcher Monash University Dr Katie Ardipradja Postdoctoral Researcher Monash University Dr Lakmali Atapattu Postdoctoral Researcher University of New South Wales Dr Michele Bastiani Senior Research Fellow University of Queensland Dr Craig Bell Postdoctoral Researcher University of Queensland Dr Quinn Besford Postdoctoral Researcher The University of Melbourne Dr Simon Brayford Postdoctoral Researcher University of New South Wales Dr Meritxell Canals Postdoctoral Researcher Monash University Dr Simona Carbone Postdoctoral Researcher Monash University Dr Chia-Chi Chen Postdoctoral Researcher University of South Australia Dr Anna Cifuentes-Rius Postdoctoral Researcher Monash University Dr Christina Cortez-Jugo Postdoctoral Researcher The University of Melbourne Dr Aidan Cousins Postdoctoral Researcher University of South Australia Dr Michael Crichton Postdoctoral Researcher University of Queensland Dr Peter Cudmore Postdoctoral Researcher The University of Melbourne Dr Robert De Rose Postdoctoral Researcher Monash University Dr Joanne Du Postdoctoral Researcher Monash University Dr Francesca Ercole Postdoctoral Researcher Monash University Dr Lars Esser Postdoctoral Researcher Monash University 72 CBNS Annual Report 2018

Dr Abbas Darestani Farahani Postdoctoral Researcher University of New South Wales

Dr Moon Sun Jung Postdoctoral Researcher University of New South Wales

Dr Michelle Maritz Postdoctoral Researcher University of South Australia

Dr Matthew Faria Postdoctoral Researcher The University of Melbourne

Dr Shereen Kadir Postdoctoral Researcher University of New South Wales

Dr Adam Martin Postdoctoral Researcher University of New South Wales

Dr Orlagh Feeney Postdoctoral Researcher Monash University

Dr Aleksandr Kakinen Postdoctoral Researcher Monash University

Dr Christian Fercher Postdoctoral Researcher University of Queensland

Professor Noritaka Kato Visiting Professor University of Melbourne

Associate Professor Joshua McCarroll Senior Research Fellow University of New South Wales

Dr Nicholas Fletcher Postdoctoral Researcher University of Queensland

Dr Pu-Chun Ke Postdoctoral Researcher Monash University

Dr Changkui Fu Postdoctoral Researcher University of Queensland

Dr Kristian Kempe NHMRC-ARC Dementia Research Development Fellow Monash University

Dr Vinicius Goncales Postdoctoral Researcher University of New South Wales Dr Thomas Hall Senior Research Fellow University of Queensland Dr Felicity Han Postdoctoral Researcher University of Queensland Dr Sifei Han Postdoctoral Researcher Monash University Dr Zachary Houston Postdoctoral Researcher University of Queensland Dr Luojuan Hu Postdoctoral Researcher Monash University Dr Pie Huda Postdoctoral Researcher University of Queensland Dr Rowan Hughes Postdoctoral Researcher University of New South Wales Dr Zil e Huma Postdoctoral Researcher Monash University Dr Daniel Hurley Postdoctoral Researcher The University of Melbourne Dr Manasi Jambhrunkar Postdoctoral Researcher University of South Australia Dr Stuart Johnston Postdoctoral Researcher The University of Melbourne Dr Paul Joyce Postdoctoral Researcher University of South Australia Dr Yi (David) Ju Postdoctoral Researcher The University of Melbourne

Dr Laura McVeigh Postdoctoral Researcher University of New South Wales Dr Angelica Merlot Postdoctoral Researcher University of New South Wales Dr Walter Muskovic Postdoctoral Researcher University of New South Wales

Dr Samantha Kristufek Postdoctoral Researcher The University of Melbourne

Dr Muneer Musthakahmed Postdoctoral Researcher University of Queensland

Dr Declan Kuch Postdoctoral Researcher University of New South Wales

Dr Tuan Nguyen Postdoctoral Researcher University of Queensland

Dr David Ladd Postdoctoral Researcher The University of Melbourne

Dr Ka Fung (Leo) Noi Postdoctoral Researcher Monash University

Dr Alistair Laos Postdoctoral Researcher University of New South Wales

Dr Anna Oszmiana Postdoctoral Researcher University of South Australia

Dr Marion Le Grand Postdoctoral Researcher University of New South Wales

Dr Stephen Parker Postdoctoral Researcher University of New South Wales

Dr Meike Leiske Postdoctoral Researcher Monash University

Dr Hui Peng Postdoctoral Researcher University of Queensland

Dr Nathania Leong Postdoctoral Researcher monash University

Dr Nghia Truong Phuoc Postdoctoral Researcher Monash University

Dr Jianhua Li Postdoctoral Researcher The University of Melbourne

Dr Daniel Poole Postdoctoral Researcher Monash University

Dr Yuhuan Li Postdoctoral Researcher Monash University

Dr Simon Puttick Postdoctoral Researcher University of Queensland

Dr Andrew Lilja Postdoctoral Researcher University of New South Wales

Dr Ruirui Qiao Postdoctoral Researcher Monash University

Dr Fabio Lisi Postdoctoral Researcher University of New South Wales

Dr Xiaole Qi Visiting Scholar Monash University

Dr Qingtao (Jason) Liu Postdoctoral Researcher Monash University

Dr Tim Quach Postdoctoral Researcher Monash University

Dr Iman Manavitehrani Postdoctoral Researcher University of New South Wales

Dr John Quinn Senior Research Fellow Monash University

Dr Friederike Mansfeld Postdoctoral Researcher University of New South Wales

Dr Md. Arifur Rahim Postdoctoral Researcher The University of Melbourne

Dr Pradeep Rajasekhar Postdoctoral Researcher Monash University

Dr Wenqian Wang Postdoctoral Researcher University of New South Wales

Mr Campbell Strong Lead Developer University of New South Wales

Ms May Lai Research Assistant Monash University

Dr Joseph J. (JJ) Richardson Postdoctoral Researcher The University of Melbourne

Dr Alessia Weiss Postdoctoral Researcher The University of Melbourne

Mr John Bailey Lead Unity Developer University of New South Wales

Mr Alistair Laos Research Assistant University of New South Wales

Dr Barbara Rolfe Senior Research Fellow University of Queensland

Dr Adam Wheatley Postdoctoral Researcher The University of Melbourne

Ms Cong Constance Li Research Assistant The University of Melbourne

Dr Iman Roohani NHMRC Fellow University of New South Wales

Dr Michael Whittaker Senior Research Fellow Monash University

Dr Alexander Soeriyadi Research Associate (NHMRC Early Career Fellow) University of New South Wales

Dr Laura Selby Postdoctoral Researcher Monash University

Dr Marnie Winter Postdoctoral Researcher University of South Australia

Dr Asif Shanavas Postdoctoral Researcher University of Queensland

Dr Jonathan Wojciechowski Postdoctoral Researcher University of New South Wales

Dr Garima Sharma Postdoctoral Researcher Monash University

Dr Alice Wong Postdoctoral Researcher University of New South Wales

Dr Priyank Shenoy Postdoctoral Researcher Monash University

Dr Chin (Ken) Wong Postdoctoral Researcher University of New South Wales

Dr Danzi Song Postdoctoral Researcher The University of Melbourne

Dr Yanfang Wu Postdoctoral Researcher University of New South Wales

Dr Hang Ta Postdoctoral Researcher University of Queensland

Dr Chih-Tsung Yang Postdoctoral Researcher University of South Australia

Dr Angel Tan Postdoctoral Researcher Monash University

Dr Ying Yang Postdoctoral Researcher University of New South Wales

Dr Hyon Xhi Tan Postdoctoral Researcher The University of Melbourne

Dr Daniel Yuen Postdoctoral Researcher Monash University

Dr Alison Tasker Postdoctoral Researcher University of Queensland

Dr Gyeongwon Yun Postdoctoral Researcher The University of Melbourne

Ms Sheilajen (Vinca) Alcantara Research Assistant The University of Melbourne

Dr Wee Siang Teo Postdoctoral Researcher University of New South Wales

Dr Cheng Zhang Postdoctoral Researcher University of Queensland

Mrs Thakshila Amarasena Research Assistant The University of Melbourne

Ms Tara Tigani Research Assistant Monash University

Dr Kristel Tjandra Postdoctoral Researcher University of New South Wales

Dr Jiacheng Zhao Postdoctoral Researcher University of Queensland

Ms Felicia Bongiovanni Research Assistant The University of Melbourne

Ms Ngoc Mai Vu Research Assistant Monash University

Dr Duy Phu Tran Postdoctoral Researcher University of South Australia

Dr Yuanhui Zheng Postdoctoral Research Fellow (UNSW Vice Chancellorâ&#x20AC;&#x2122;s) University of New South Wales

Ms Moore Chen Research Assistant Monash University

Dr Alessia Weiss Research Assistant The University of Melbourne

Ms Varvara Efremova Research Assistant University of New South Wales

Mr Anthony Wignall Research Assistant University of South Australia

Ms Robyn Esterbauer Research Assistant The University of Melbourne

Mr James Wilmot Research Assistant University of New South Wales

Ms Arisbel Batista Gondin Research Assistant Monash University

Ms Jenny Tran Research Assistant/Laboratory Coordinator The University of Melbourne

Dr Nicholas Veldhuis Postdoctoral Researcher Monash University Dr Orazio Vittorio Postdoctoral Researcher (Cancer Institute NSW ECR Fellow) University of New South Wales

Dr Jiajing Zhou Postdoctoral Researcher The University of Melbourne Dr Joshua McCarroll Project Leader University of New South Wales Mr Charles Ferguson Immunoem Facility Manager University of Queensland Dr Susan Nixon Lab Manager University of Queensland

Dr Ernesto Moles Research Officer University of New South Wales Dr Hanna Gustafsson Research Associate University of South Australia Dr Johan Gustafsson Research Associate University of South Australia Dr Katharina Richter Research Associate University of South Australia Ms Kathleen Kimpton Senior Research Assistant University of New South Wales Ms Rose-Marie Olsson Senior Research Assistant University of New South Wales Ms Christiana (Christie) Agyei-Yeboah Research Assistant University of Queensland

Mr Ben Gubbins Research Assistant The University of Melbourne

Mr Christopher Lumb Research Assistant Monash University Mr Nick Martel Research Assistant University of Queensland Ms Georgia Miller Research Assistant University of New South Wales Ms Aparna Nandakumar Research Assistant Monash University Mr Karl Peressin Research Assistant University of South Australia Ms Georgia Porter Research Assistant University of New South Wales Mr James Rae Research Assistant University of Queensland Ms Ayame Saito Research Assistant Monash University Mrs Sona Samuels Research Assistant University of New South Wales Ms Danielle Senyschyn Research Assistant Monash University

CBNS Annual Report 2018 73

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Dr Robert Utama Postdoctoral Researcher University of New South Wales

Dr Nicky Thomas Research Associate (NHMRC Research Fellow) University of South Australia

Ms Sibylle BriÃre Intern Monash University Mr Ryan Clayden-Zabik Intern University of Queensland Mr Louis-Manuel Layani Intern Monash University Ms Aline Martins dos Santos Intern University of Queensland Ms Jessica Mauriello Intern Monash University Ms Joyce Tang Intern University of Queensland

Students PhD students Mr Hazem Abdelmaksoud University of South Australia Ms Dewan Ahkter University of Queensland Ms Salma Ahmed University of Queensland Ms Aria Ahmed-Cox University of New South Wales Ms Nabila Akhtar Monash University Mr Karrar Al-Taief University of New South Wales Ms Yassamin Albayaty University of South Australia Mr Nicolas Alcaraz Monash University Ms Fida’a Alshawawreh University of New South Wales Ms Tara Alvarez Monash University Mr Adit Ardana University of Queensland Mr Md. Toasin Hossain Aunkor Monash University

Mr Simone Bonaccorsi University of New South Wales

Ms Sachini Fonseka University of Queensland

Mr Zhengyang Jia University of South Australia

Ms Caterina Brighi University of Queensland

Ms Chelsea Forest University of New South Wales

Mr Zhen Jiang University of Queensland

Mr Daniel Brundel Monash University

Ms Helen Forgham University of New South Wales

Ms Mohadesseh Kahram University of New South Wales

Ms Enyuan Cao Monash University

Ms Bruna Cambraia Garms University of Queensland

Ms Hannah Kelly The University of Melbourne

Mr Lachlan Carter University of New South Wales

Ms Anna Gemmell University of Queensland

Mr Ujjaval Kerketta University of New South Wales

Dr David Chang University of New South Wales

Mr Mohammadreza Alizadeh Ghodsi University of South Australia

Ms Vidhishri Kesarwani Monash University

Ms Dongfei Chen University of New South Wales Ms Jingqu (Rachel) Chen The University of Melbourne Ms Xueqian Chen University of New South Wales Ms Rinku Chhasatia University of South Australia Ms Evon Chong Monash University Mr Joshua Conner Monash University Ms Ewa Czuba-Wojnilowicz The University of Melbourne Mr Nam Dao Monash University Mr Pouya Dehghankelishadi University of South Australia Ms Ludivine Delon University of South Australia Ms Tahnee Dening University of South Australia Mr Jesse Di Cello Monash University Mr Eric Du University of New South Wales Ms Joanne Du Monash University Ms Genevieve Duche University of New South Wales Ms Gayathri Ediriweera University of Queensland

Mr Stefan Bader Monash University

Ms Mona Elsemary University of South Australia

Ms Parisa Badiee University of South Australia

Mr Gerald Er University of Queensland

Mr Arunpandian Balaji University of Queensland

Mr Sanjun Fan University of New South Wales

Mr Gregory Bass The University of Melbourne

Mr Abbas Darestani Farahani University of New South Wales

Mr Daniel Bobo University of Queensland

Mr Matthew Faria The University of Melbourne Ms Ava Faridi Monash University

74 CBNS Annual Report 2018

Ms Agata Glab The University of Melbourne Mr Joshua Glass The University of Melbourne Mr James Grace Monash University Ms Gracia Gracia Monash University Mr Zhaobin Guo University of South Australia Mr Fei Han University of New South Wales Ms Han Han University of New South Wales Ms Yiyuan Han The University of Melbourne Mr Md. Musfizur Hassan University of New South Wales Mr Edward Charles Henderson Monash University Ms Linda Hong Monash University Ms Sharmin Hoque University of New South Wales Mr Douglas Howard University of South Australia Ms Yingjie Hu The University of Melbourne Mr Lei Huang The University of Melbourne Mr James Humphries University of Queensland Mr Gabriel Huynh Monash University Ms Susan Ireland University of New South Wales Mr Jiaul Islam Monash University Ms Nilou Jamshidi University of New South Wales Mr Ibrahim Javed Monash University

Mr Song Yang Khor Monash University Ms Ana Klisuric University of South Australia Ms Ruby Kochappan Monash University Mr John Kokkinos University of New South Wales Ms Giri Krishnan University of South Australia Ms May Lai Monash University Mr Fayaz Ali Larik University of New South Wales Mr Hong (Nick) Seng Lee University of Queensland Ms Given Lee Monash University Ms Shiyao Li The University of Melbourne Ms Tang Li Monash University Ms Jiaxin (Lily) Lian University of New South Wales Mr Ye-Wheen Lim University of Queensland Mr Gan Lin The University of Melbourne Mr Zhixing Lin The University of Melbourne Mr Yi Liu The University of Melbourne Ms Joanne Ly Monash University Mr Yutian Ma The University of Melbourne Ms Zerong (Shirley) Ma University of New South Wales Ms Saji Maghrebi University of South Australia Ms Quynh Mai Monash University Mr Milad Mehdipour University of New South Wales

Ms Tahlia Meola University of South Australia

Mr Joshua Rennick Monash University

Mr Miaoyi (Marvin) Wang Monash University

Ms Kalista Clout-Holding Monash University

Ms Valentina Milanova University of South Australia

Mr Meysam Rezaeishahmirzadi University of South Australia

Mr Hareth Wassiti Monash University

Mr Kien Duong Monash University

Mr Jonathan Wei University of Queensland

Ms Shauna Herbst University of Queensland

Ms Alessia Weiss The University of Melbourne

Mr David Hoang Monash University

Mr Jeffri Retamal Santibanez Monash University

Mr Nicholas Westra van Holthe University of Queensland

Ms Ada Huang Monash University

Mrs Shebbrin Moonshi University of Queensland

Ms Jennifer Schoning University of Queensland

Mr Marcin Wojnilowicz The University of Melbourne

Ms Emilia Morris University of South Australia

Ms Hayley Schultz University of South Australia

Mr Chin (Ken) Wong University of New South Wales

Mr Walter Muskovic University of New South Wales

Mr Joshua Simpson University of Queensland

Ms Leah Wright University of South Australia

Mr Hweeing Ng University of Queensland

Mr Amal Sivaram University of Queensland

Mr Yanfang Wu University of New South Wales

Mr Thanh Nguyen Monash University

Ms Vanessa Soh University of Queensland

Ms Yunxin (Cindy) Xiao Monash University

Ms Duyen Nguyen University of New South Wales

Ms Danzi Song The University of Melbourne

Ms Fangyun Xin Monash University

Ms Vy Nguyen University of South Australia

Ms Jiaying Song The University of Melbourne

Mr Ken Yong Monash University

Mr Pietro Pacchin Tomanin The University of Melbourne

Ms Parisa Sowti University of New South Wales

Ms Sul (Elly) Yu Monash University

Ms Roberta Pala University of New South Wales

Mr Manish Sriram University of New South Wales

Ms Leila Zarei University of New South Wales

Mr Shuaijun Pan The University of Melbourne

Mr Ian Styles Monash University

Mr Wenjie Zhang The University of Melbourne

Ms Raheleh Pardekhorram University of New South Wales

Ms Xiaohan Sun Monash University

Ms Manchen Zhao University of New South Wales

Ms Amelia Parker University of New South Wales

Ms Estelle Suys Monash University

Mr Qizhi Zhong The University of Melbourne

Mr Scott Peng Monash University

Ms Wenxian Tang University of New South Wales

Ms Xiaotong Zhou Monash University

Ms Thuy Pham University of South Australia

Ms Serena Teo Monash University

Mr Minze Zhu University of Queensland

Ms Emily Pilkington Monash University

Mr Cong Vu Thanh University of New South Wales

Ms Aadarsh Zia Monash University

Ms Ranjana Piya University of New South Wales

Ms Chelsea Thorn University of South Australia

Ms Kelly Zong University of New South Wales

Mr Bijan Po University of New South Wales

Ms Kristel Tjandra University of New South Wales

Dr Maryline Chee Kimling Node Coordinator The University of Melbourne

Master students

Ms Carla Gerbo Executive Assistant University of Queensland

Mr Alexander Moersdorf Monash University Ms Ayaat Mahmoud Mohamed Monash University

Mr Kye Robinson Monash University Ms Bilquis Romana University of South Australia

Ms Yijiao Qu The University of Melbourne

Mr Matthew Urquhart Monash University

Mr Sayem Rahman University of New South Wales

Mr Adil Usman University of Queensland

Ms Paulina RamĂrez-GarcĂa Monash University

Ms Nicole van der Burg University of Queensland

Dr Gishan Ratnayake University of Queensland

Ms Florida Voli University of New South Wales Ms Julia Walker Monash University

Mr Yuanbin Lu University of Queensland

Ms Jia Kuah Monash University Ms Yuen Li (Jamie) Lam Monash University Mr Wen Mow Monash University Ms Kara Paxton University of South Australia Ms Daniela Hidalgo Rocha University of Queensland Mr Ronny Sanchez University of Queensland Mr Matthew Sims University of New South Wales Ms Santhni Subramaniam University of South Australia Mr Johnson Yao Monash University Ms Ching-min (Mindy) Yeh University of Queensland

Administrative staff Dr Natalie Jones Centre Manager Monash University Ms Anne Meyer Senior Coordinator Communications and Events Monash University Dr Charlotte Hurry Centre Administrator Monash University

Ms Ngoc Mai Vu Monash University Mr Yuao Wu University of Queensland

p e r fo r man c e

Ms Parisa Moazzam University of New South Wales

Honours students Ms Danielle Bennett University of New South Wales

CBNS Annual Report 2018 75

Visitors to CBNS Visitors from National Organisations

Visitors from international organisations

Dr Renee Goreham The MacDiarmid Institute, Victoria University of Wellington, NZ

Dr Maria Alba Martin Monash University, Australia

Professor Cameron Alexander University of Nottingham, UK

Professor Jonathan Baell Monash University, Australia

Professor Bradley Anderson University of Kentucky, USA

Dr Jan Grimm Memorial Sloan Kettering Cancer Center, USA

Dr Thomas Cox Garvan Institute of Medical Research, Australia

Professor Joakim Andreasson Chalmers University of Technology, Sweden

Dr Sushil Dhital University of Queensland, Australia Professor Peter Gregory Editor-in-chief in Chief Advanced Materials, Australia

Associate Professor Christel Bergström University of Uppsala, Sweden Dr Sofi Bin-Salamon US Air Force Office of Scientific Research and Texas A&M University, USA

Ms Rui Guo Hunan University, China Dr Philipp Gutruf The University of Arizona, USA Leonie Van’t Hag ETH Zurich, Switzerland Professor Chaoliang He Chinese Academy of Sciences, China

Professor Hans Bräuner-Osborne University of Copenhagen, Denmark

Dr Roshanak Irranejad University of California, San Francisco, USA

Ms Sibylle Brière CPE Lyon, France

Professor Derek Irvine University of Nottingham, UK

Professor Michael Kassiou The University of Sydney, Australia

Distinguished Professor Margaret Brimble The University of Auckland, NZ

Associate Professor Daisuke Ishii Nagoya Institute of Technology, Japan

Dr Andrew Keller Monash University, Australia

Professor Weibo Cai University of Wiscosin–Madison, USA

Dr Katie Leach Monash University, Australia

Professor Warren Chan University of Toronto, Canada

Dr Lynn Liang Monash University, Australia

Professor Hong C Chen Soochow University, China

Professor Lei Jiang University of Chinese Academy of Sciences, China

Dr Christopher MacRaild Monash University, Australia

Professor Xuesi Chen Chinese Academy of Sciences, China

Dr Peter Karsark Qatar University, Qatar

Dr Simon McKenzie-Nickson Monash University, Australia

Professor Karen Cheung University of British Columbia, Canada

Professor Norikato Kato Meiji University, Japan

Dr Srinivas Mettu University of Melbourne, Australia

Associate Professor Susana I. Córdoba de Torresi University of São Paulo, Brazil

Professor Rafal Klajn Weizmann Institute of Science, Israel

Dr Liang Jin Monash University, Australia Dr Swapna Johnson Monash University, Australia

Dr Cameron Nowell Monash University, Australia Professor Michael Parker Bio21 Molecular Science and Biotechnology Institute, Australia

Dr Liming Dai Case Western Reserve University, USA Dr James Dekker Fonterra, NZ

Dr Beatriz Prieto-Simon Monash University, Australia

Dr Brad Doak Uppsala University, Sweden

Professor Roland Stocker Victor Chang Cardiac Research Institute, Australia

Ms Hui Du Du Soochow University, China

Dr Paul Stupple Monash University, Australia Dr Natalie Trevaskis Monash University, Australia

76 CBNS Annual Report 2018

Ms Ruyan Feng Soochow University, China Professor Andreas Fery Leibniz Institut für Polymerforschung Dresden, Germany

Ms Lyna Janvier ENSCM, France Professor Sheila Jasanoff Harvard University, USA

Professor András Komáromy Michigan State University, USA Dr Vasileios Kontogiorgos University Of Huddersfield, UK Dr Jason Lewis Memorial Sloan Kettering Cancer Center, USA Professor Jason Lewis Memorial Sloan Kettering Cancer Center, USA Ms Shanshan Li Sichuan University, China Professor Dan Li Soochow University, China

Professor Amar Flood Indiana University, USA

Dr Mathias Lindquist University Copenhagen, Denmark

Dr Sophie Gallier Fonterra, NZ

Associate Professor Xiaoli Liu Soochow University, China

Ms Yan Luo Soochow University, China

Professor Dan Peer University of Tel Aviv, Israel

Professor Michael S. Silverstein Technion Israel Institute of Technology, Israel

Professor Melissa Mather University of Nottingham, UK

Professor Dan Peer Tel Aviv University, Israel

Sir Fraser Stoddart Northwestern University, USA

Professor Satyajit Mayor National Centre for Biological Sciences, India

Dr Guotao (Sunny) Peng Tongji University, China

Ms Farsai Taemaitree Tohoku University, Japan

Professor Sébastien Perrier University of Warwick, UK

Professor Zhaohui Tang Chinese Academy of Sciences, China

Tasnim Pharmaceuticals, Iran

Professor Huayu Tian Chinese Academy of Sciences, China

Dr Sara Miller University Otago, NZ

Professor Louis Picker Oregon Health & Science University, USA

Professor Thomas Nann The MacDiarmid Institute, Victoria University of Wellington, NZ

Ms Anika Prinz Saarland University, Germany

Professor Osvaldo Novais de Oliveria Jr University of São Paulo, Brazil Professor Tommy Nylander Lund Univerity, Sweden Professor David O’Connor University of Wiscosin–Madison, USA Professor Shelby O’Connor University of Wiscosin–Madison, USA Dr Amanda Pearce University of Nottingham, UK Dr Martin Peeks Massachusetts Institute of Technology, USA

Professor Addy Pross Ben Gurion University of Negev, Israel Professor Robert K. Prud’homme Princeton University, USA Ms Kate Reidy University of Dublin, Ireland Associate Professor Gregory Scherrer Stanford University, USA Associate Professor Timothy F. Scott University of Michigan, USA Dr Asifkhan Shanavas Institute for Nanoscience and Technology, Mohali, India

Professor Bernhard Wolfrum Technical University Munich, Germany Professor Chunsheng Xiao Chinese Academy of Sciences, China Professor Qian Yu Soochow University, China Professor Marino Zerial Max Planck Institute, Dresden, Germany Ms Yu Zhang Chinese Academy of Sciences, China Dr Yongmei Zhao Nantong University, China Professor Shemin Zhu Shanghai Jiaotong University, China

p e r fo r man c e

Professor Herbert Mayr Ludwig Maximilian University of Munich, Germany

CBNS Annual Report 2018 77

Publications Books and Book Chapters Anastasaki A., Whitfield R, Nikolaou V., Truong N.P., Jones G.R, Engelis N.G, Liarou E., Whittaker M.R., Haddleton D.M., One-Pot Sequence-Controlled (SC) Multiblock Copolymers via Copper-Mediated Polymerization, Sequence-Controlled Polymers, pp 417-434, Wiley-VCH Verlag GmbH & Co. KGaA Gupta B., Gooding J.J., 2018, Biomolecule Attachment to Porous Silicon, Handbook of Porous Silicon Springer, Cham Low S.P., Voelcker N.H., 2018, Biocompatibility of Porous Silicon, Handbook of Porous Silicon, Springer, Cham McInnes S.J.P., Turner C.T., Cowin A.J., Voelcker N.H., 2018, Wound Management Using Porous Silicon, Handbook of Porous Silicon, Springer, Cham Voelcker N.H., Low S.P., ,2018, Cell Culture on Porous Silicon, Handbook of Porous Silicon, Springer, Cham

Conference Proceedings Sharbeen G., Akerman A., McCarroll J., Boyer C., Holst J., Youkhana J., Metabolically Reprograming Pancreatic Stellate Cells Using a Novel Nanomedicine for the Treatment of Pancreatic Cancer, 49th Annual Meeting of the American Pancreatic Association Atapattu L., Utama R., O’Mahony A., Fife C., Baek J., Allard T., O’Mahony K., Ribeiro J., Gaus K., Gooding J., Kavallaris M., Precision medicine: Highthroughput 3D bioprinting of embedded multicellular cancer spheroids, Cancer Research Carbone S., DiCello J., Saito A., Szymaszkiewicz A., Harnessing endogenous opioids to control gastrointestinal dysmotility: The case for allosteric modulation Neurogastroenterology and Motility 2018 Dicello J., Saito A., McQuade R., Sebastian B., Canals M., The effects of prolonged dosage of delta opioid receptor agonists on colonic motility in the mouse: Analgesics without GI motility-associated side effects? Neurogastroenterology and Motility 2018

78 CBNS Annual Report 2018

Forgham H., Esser L., Quinn J.F., Mansfeld F., Slapetova L., Whan R., Ariotti N., Davis T.P., Kavallaris M., McCarroll J., Published Abstract, European Society for Medical Oncology 2018 Congress Open Forgham H., EsserJ., Quinn J.F., Mansfeld F., Slapetova I., Whan R., Ariotti N., PO-354 Star-shaped gene silencing nanoparticles–an advanced approach to treating medulloblastoma, European Society for Medical Oncology 2018 Congress Open 3 (Suppl 2), A367-A368 Goos J., Puttick S., Dilling T., Gupta A., Price W., Quinn J., Whittaker M., Star polymers for PET/MRI: a passive pretargeting approach, Journal of Nuclear Medicine 59 (supplement 1), 195-195 Kokkinos J., Sharbeen G., McCarroll J., Boyer C., Akerman A., Youkhana J., beta III-Tubulin is a Brake for External Cell-Death Signaling and Regulates TRAIL Sensitivity in Pancreatic Cancer, 49th Annual Meeting of the American Pancreatic Association, 1401-1401 Kristufek S., Richardson J., Reidy K., Quinn J., Davis T., Caruso F., Utilising the chemical toolbox for the synthesis of catecholbased polymers for the assembly of functional metal phenolic networks, Abstracts of papers of the American Chemical Society 256 Rajasekhar P., Carbone S.E., Nowell C.J., Fichna J., Identification of novel sites of TRPV4 expression in the mouse colon Neurogastroenterology and Motility 2018 Roy M., Sarwat S., O’Mara P., Almaimani M., Tilley R., Gooding J., Willcox M., Stapleton F., Imaging of tear film lipids using quantum dots, Optics InfoBase Conference 2018, Papers Voli F., Lerra L., Kimpton K., Saletta F., Cirillo G., Kavallaris M., Vittorio O., Harnessing copper in cancer to enhance anti-tumour immune response, Journal Annals of Oncology: 99P

Journal Entries Albayaty Y.N., Thomas N., Hasan S., Prestidge C.A., Penetration of topically used antimicrobials through Staphylococcus aureus biofilms: A comparative study using different models, Journal

of Drug Delivery Science and Technology, 2018, 48, 429-436 Aldilla V.R., Martin A.D., Nizalapur S., Marjo C.E., Rich A.M., Ho K.K.K., Ittner L.M., Black D.S., Thordarson P., Kumar N., Glyoxylamide-based selfassembly hydrogels for sustained ciprofloxacin delivery, Journal of Materials Chemistry B, 2018, 6, 6089-6098 Ali I., Rehman J.U., Ullah S., Imran M., Javed I., El-Haj B.M., Saad Ali H., Arfan M., Shah M.R., Preliminary investigation of novel tetra-tailed macrocycle amphiphile based nano-vesicles for amphotericin B improved oral pharmacokinetics, Artificial Cells, Nanomedicine and Biotechnology, 2018 Ali I., Shah M.R., Yousuf S., Ahmed S., Shah K., Javed I., Hemolytic and cellular toxicology of a sulfanilamide-based nonionic surfactant: A niosomal carrier for hydrophobic drugs, Toxicology Research, 2018, 7, 771-778 Anania J.C., Trist H.M., Palmer C.S., Tan P.S., Kouskousis B.P., Chenoweth A.M., Kent S.J., Mackay G.A., Hoi A., Koelmeyer R., Slade C., Bryant V.L., Hodgkin P.D., Aui P.M., van Zelm M.C., Wines B.D., Hogarth P.M., The Rare Anaphylaxis-Associated FcγRIIa3 Exhibits Distinct Characteristics From the Canonical FcγRIIa1, Frontiers in Immunology, 2018, 9, 1809 Barbon S.M., Rolland M., Anastasaki A., Truong N.P., Schulze M.W., Bates C.M., Hawker C.J., Macrocyclic Side-Chain Monomers for Photoinduced ATRP: Synthesis and Properties versus Long-Chain Linear Isomers, Macromolecules, 2018, 51, 6901-6910 Belfiore L., Saunders D.N., Ranson M., Thurecht K.J., Storm G., Vine K.L., Towards clinical translation of ligandfunctionalized liposomes in targeted cancer therapy: Challenges and opportunities, Journal of Controlled Release, 2018, 277, 1-13 Benedetti T.M., Andronescu C., Cheong S., Wilde P., Wordsworth J., Kientz M., Tilley R.D., Schuhmann W., Gooding J.J., Electrocatalytic Nanoparticles That Mimic the Three-Dimensional Geometric Architecture of Enzymes:

Nanozymes, Journal of the American Chemical Society, 2018, 140, 13449-13455 Bennett T.M., Chambers L.C., Thurecht K.J., Jack K.S., Blakey I., Dependence of Block Copolymer Domain Spacing and Morphology on the Cation Structure of Ionic Liquid Additives, Macromolecules, 2018, 51, 8979-8986 Bertucci A., Guo J., Oppmann N., Glab A., Ricci F., Caruso F., Cavalieri F., Probing transcription factor binding activity and downstream gene silencing in living cells with a DNA nanoswitch, Nanoscale, 2018, 10, 2034-2044 Besford Q.A., Ju Y., Wang T.-Y., Yun G., Cherepanov P., Hagemeyer C.E., Cavalieri F., Caruso F., Self-Assembled Metal–Phenolic Networks on Emulsions as Low-Fouling and pH-Responsive Particles, Small, 2018, 14 Bidram E., Sulistio A., Cho H.-J., Amini A., Harris T., Zarrabi A., Qiao G., Stewart A., Dunstan D.E., Targeted Graphene Oxide Networks: Cytotoxicity and Synergy with Anticancer Agents, ACS Applied Materials and Interfaces, 2018, 10, 43523-43532 Bisset N.B., Webster G.R., Dong Y.D., Boyd B.J., Understanding the kinetic mixing between liquid crystalline nanoparticles and agrochemical actives, Colloids and Surfaces B: Biointerfaces, 2019, 175, 324-332 Björnmalm M., Caruso F., Robust Chemistry: The Importance of Data and Methods Sharing, Angewandte Chemie – International Edition, 2018, 57, 1122-1123 Blackman L.D., Varlas S., Arno M.C., Houston Z.H., Fletcher N.L., Thurecht K.J., Hasan M., Gibson M.I., O’Reilly R.K., Confinement of Therapeutic Enzymes in Selectively Permeable Polymer Vesicles by PolymerizationInduced Self-Assembly (PISA) Reduces Antibody Binding and Proteolytic Susceptibility, ACS Central Science, 2018, 4, 718-723 Boase N.R.B., Torres M.D.T., Fletcher N.L., De La FuenteNunez C., Fairfull-Smith K.E., Polynitroxide copolymers to reduce biofilm fouling on surfaces, Polymer Chemistry, 2018, 9, 5308-5318

Boyd B.J., Salim M., Clulow A.J., Ramirez G., Pham A.C., Hawley A., The impact of digestion is essential to the understanding of milk as a drug delivery system for poorly water soluble drugs, Journal of Controlled Release, 2018, 292, 13-17 Bremmell K.E., Prestidge C.A., Enhancing oral bioavailability of poorly soluble drugs with mesoporous silica based systems: opportunities and challenges, Drug Development and Industrial Pharmacy, 2018 Brighi C., Puttick S., Rose S., Whittaker A.K., The potential for remodelling the tumour vasculature in glioblastoma, Advanced Drug Delivery Reviews, 2018, 136-137, 49-61 Cao Z., Adnan N.N.M., Wang G., Rawal A., Shi B., Liu R., Liang K., Zhao L., Gooding J.J., Boyer C., Gu Z., Enhanced colloidal stability and protein resistance of layered double hydroxide nanoparticles with phosphonic acid-terminated PEG coating for drug delivery, Journal of Colloid and Interface Science, 2018, 521, 242-251 Cao Z., Zhang L., Liang K., Cheong S., Boyer C., Gooding J.J., Chen Y., Gu Z., Biodegradable 2D Fe–Al Hydroxide for Nanocatalytic Tumor-Dynamic Therapy with Tumor Specificity, Advanced Science, 2018, 5 Carroll T.D., Jegaskanda S., Matzinger S.R., Fritts L., McChesney M.B., Kent S.J., Fairman J., Miller C.J., A lipid/ DNA adjuvant-inactivated influenza virus vaccine protects rhesus macaques from uncontrolled virus replication afer heterosubtypic influenza a virus challenge, Journal of Infectious Diseases, 2018, 218, 856-867 Chang D., Lim M., Goos J.A.C.M., Qiao R., Ng Y.Y., Mansfeld F.M., Jackson M., Davis T.P., Kavallaris M., Biologically targeted magnetic hyperthermia: Potential and limitations, Frontiers in Pharmacology, 2018, 9 Cheeseman H.M., Day S., McFarlane L.R., Fleck S., Miller A., Cole T., Sousa-Santos N., Cope A., Cizmeci D., Tolazzi

M., Hwekwete E., Hannaman D., Kratochvil S., McKay P.F., Chung A.W., Kent S.J., Cook A., Scarlatti G., Abraham S., Combadiere B., McCormack S., Lewis D.J., Shattock R.J., Combined Skin and Muscle DNA Priming Provides Enhanced Humoral Responses to a Human Immunodeficency Virus Type 1 Clade C Envelope Vaccine, Human Gene Therapy, 2018, 29, 1011-1028 Chen L., Glass J.J., De Rose R., Sperling C., Kent S.J., Houston Z.H., Fletcher N.L., Rolfe B.E., Thurecht K.J., Influence of Charge on Hemocompatibility and Immunoreactivity of Polymeric Nanoparticles, ACS Applied Bio Materials, 2018 Cherepanov P.V., Rahim M.A., Bertleff-Zieschang N., Sayeed M.A., O’Mullane A.P., Moulton S.E., Caruso F., Electrochemical Behavior and Redox-Dependent Disassembly of Gallic Acid/FeIII Metal-Phenolic Networks, ACS Applied Materials and Interfaces, 2018, 10, 5828-5834 Chhasatia R., Sweetman M.J., Prieto-Simon B., Voelcker N.H., Performance optimisation of porous silicon rugate filter biosensor for the detection of insulin, Sensors and Actuators, B: Chemical, 2018, 273, 1313-1322 Chu Y., Hou J., Boyer C., Richardson J.J., Liang K., Xu J., Biomimetic synthesis of coordination network materials: Recent advances in MOFs and MPNs, Applied Materials Today, 2018, 10, 93-105 Clulow A.J., Salim M., Hawley A., Boyd B.J., A closer look at the behaviour of milk lipids during digestion, Chemistry and Physics of Lipids, 2018, 211, 107-116 Clulow A.J., Salim M., Hawley A., Gilbert E.P., Boyd B.J., The Curious Case of the OZ439 Mesylate Salt: An Amphiphilic Antimalarial Drug with Diverse Solution and Solid State Structures, Molecular Pharmaceutics, 2018, 15, 2027-2035

Expert Opinion on Drug Delivery, 2018, 15, 881-892 Corrie S.R., Plebanski M., The emerging role of nanomaterials in immunological sensing – a brief review, Molecular Immunology, 2018, 98, 28-35 Cui C., Faraji N., Lauto A., Travaglini L., Tonkin J., Mahns D., Humphrey E., Terracciano C., Gooding J.J., Seidel J., Mawad D., A flexible polyaniline-based bioelectronic patch, Biomaterials Science, 2018, 6, 493-500 Cui J., Björnmalm M., Ju Y., Caruso F., Nanoengineering of Poly(ethylene glycol) Particles for Stealth and Targeting, Langmuir, 2018, 34, 10817-10827 Cupic K.I., Rennick J.J., Johnston A.P., Such G.K., Controlling endosomal escape using nanoparticle composition: Current progress and future perspectives, Nanomedicine, 2019, 14, 215-223 Cursons J., Pillman K.A., Scheer K.G., Gregory P.A., Foroutan M., Hediyeh-Zadeh S., Toubia J., Crampin E.J., Goodall G.J., Bracken C.P., Davis M.J., Combinatorial Targeting by MicroRNAs Co-ordinates Post-transcriptional Control of EMT, Cell Systems, 2018, 7, 77-910000000 Dai Q., Bertleff-Zieschang N., Braunger J.A., Björnmalm M., Cortez-Jugo C., Caruso F., Particle Targeting in Complex Biological Media, Advanced Healthcare Materials, 2018, 7 Dai X., Ge D., Numerical investigation on the field emission properties of N-doped graphdiyne-C60 nanostructures, AIP Advances, 2018, 8 de Jongh P.A.J.M., Haddleton D.M., Kempe K., Spontaneous zwitterionic copolymerisation: An undervalued and efficacious technique for the synthesis of functional degradable oligomers and polymers, Progress in Polymer Science, 2018, 87, 228-246

Coffey J.W., Corrie S.R., Kendall M.A.F., Rapid and selective sampling of IgG from skin in less than 1 min using a high surface area wearable immunoassay patch, Biomaterials, 2018, 170, 49-57

Dening T.J., Joyce P., Webber J.L., Beattie D.A., Prestidge C.A., Inorganic surface chemistry and nanostructure controls lipolytic product speciation and partitioning during the digestion of inorganic-lipid hybrid particles, Journal of Colloid and Interface Science, 2018, 532, 666-679

Cong V.T., Gaus K., Tilley R.D., Gooding J.J., Rod-shaped mesoporous silica nanoparticles for nanomedicine: recent progress and perspectives,

Dening T.J., Thomas N., Rao S., Van Looveren C., Cuyckens F., Holm R., Prestidge C.A., Montmorillonite and Laponite Clay Materials for the

Solidification of Lipid-Based Formulations for the Basic Drug Blonanserin: In Vitro and in Vivo Investigations, Molecular Pharmaceutics, 2018, 15, 4148-4160 di Luca M., Vittorio O., Cirillo G., Curcio M., Czuban M., Voli F., Farfalla A., Hampel S., Nicoletta F.P., Iemma F., Electro-responsive graphene oxide hydrogels for skin bandages: The outcome of gelatin and trypsin immobilization, International Journal of Pharmaceutics, 2018, 546, 50-60 Dicello J.J., Saito A., Rajasekhar P., Eriksson E.M., McQuade R.M., Nowell C.J., Sebastian B.W., Fichna J., Veldhuis N.A., Canals M., Bunnett N.W., Carbone S.E., Poole D.P., Hormones, neurotransmitters, growth factors, receptors, and signaling: Inflammationassociated changes in DOR expression and function in the mouse colon, American Journal of Physiology – Gastrointestinal and Liver Physiology, 2018, 315, G544-G559 Du J.D., Hong L., Tan A., Boyd B.J., Naphthalocyanine as a New Photothermal Actuator for LipidBased Drug Delivery Systems, Journal of Physical Chemistry B, 2018, 122, 1766-1770 Esser L., Lengkeek N.A., Moffat B.A., Vu M.N., Greguric I., Quinn J.F., Davis T.P., Whittaker M.R., A tunable one-pot threecomponent synthesis of an 125I and Gd-labelled star polymer nanoparticle for hybrid imaging with MRI and nuclear medicine, Polymer Chemistry, 2018, 9, 3528-3535 Faria M., Björnmalm M., Thurecht K.J., Kent S.J., Parton R.G., Kavallaris M., Johnston A.P.R., Gooding J.J., Corrie S.R., Boyd B.J., Thordarson P., Whittaker A.K., Stevens M.M., Prestidge C.A., Porter C.J.H., Parak W.J., Davis T.P., Crampin E.J., Caruso F., Minimum information reporting in bio-nano experimental literature, Nature Nanotechnology, 2018, 13, 777-785 Faridi A., Sun Y., Okazaki Y., Peng G., Gao J., Kakinen A., Faridi P., Zhao M., Javed I., Purcell A.W., Davis T.P., Lin S., Oda R., Ding F., Ke P.C., Mitigating Human IAPP Amyloidogenesis In Vivo with Chiral Silica Nanoribbons, Small, 2018, 14 Fletcher N.L., Houston Z.H., Simpson J.D., Veedu R.N., Thurecht K.J., Designed multifunctional polymeric nanomedicines: Long-term CBNS Annual Report 2018 79

p e r fo r man c e

Bonnard T., Jayapadman A., Putri J.A., Cui J., Ju Y., Carmichael C., Angelovich T.A., Cody S.H., French S., Pascaud K., Pearce H.A., Jagdale S., Caruso F., Hagemeyer C.E., Low-Fouling and Biodegradable ProteinBased Particles for Thrombus Imaging, ACS Nano, 2018, 12, 6988-6996

biodistribution and tumour accumulation of aptamertargeted nanomaterials, Chemical Communications, 2018, 54, 11538-11541 Forgham H., Esser L., Quinn J.F., Mansfeld F., Slapetova I., Whan R., Ariotti N., Davis T.P., PO-354 Star-shaped gene silencing nanoparticles– an advanced approach to treating medulloblastoma, European Society for Medical Oncology 2019 Congress Open, 2018 Freires A.S., dos Reis Lima F.M., Yotsumoto-Neto S., Silva S.M., Damos F.S., de Cássia Silva Luz R., Exploiting CdSe/ZnS coreshell photocatalyst modified with cytochrome c for epinephrine determination in drugs utilized in cardiopulmonary resuscitation, Microchemical Journal, 2018, 139, 18-23 Fu C., Zhang C., Peng H., Han F., Baker C., Wu Y., Ta H., Whittaker A.K., Enhanced Performance of Polymeric 19F MRI Contrast Agents through Incorporation of Highly Water-Soluble Monomer MSEA, Macromolecules, 2018, 51, 5875-5882 Furtado D., Björnmalm M., Ayton S., Bush A.I., Kempe K., Caruso F., Overcoming the Blood–Brain Barrier: The Role of Nanomaterials in Treating Neurological Diseases, Advanced Materials, 2018, 30 Ghosh S., Tran K., Delbridge L.M.D., Hickey A.J.R., Hanssen E., Crampin E.J., Rajagopal V., Insights on the impact of mitochondrial organisation on bioenergetics in high-resolution computational models of cardiac cell architecture, PLoS Computational Biology, 2018, 14, e1006640 Gloag L., Benedetti T.M., Cheong S., Li Y., Chan X.-H., Lacroix L.-M., Chang S.L.Y., Arenal R., Florea I., Barron H., Barnard A.S., Henning A.M., Zhao C., Schuhmann W., Gooding J.J., Tilley R.D., Three-Dimensional Branched and Faceted Gold– Ruthenium Nanoparticles: Using Nanostructure to Improve Stability in Oxygen Evolution Electrocatalysis, Angewandte Chemie – International Edition, 2018, 57, 10241-10245 Gloag L., Benedetti T.M., Cheong S., Webster R.F., Marjo C.E., Gooding J.J., Tilley R.D., Pd-Ru core-shell nanoparticles with tunable shell thickness for active and stable oxygen evolution performance, Nanoscale, 2018, 10, 15173-15177

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Grace J.L., Schneider-Futschik E.K., Elliott A.G., Amado M., Truong N.P., Cooper M.A., Li J., Davis T.P., Quinn J.F., Velkov T., Whittaker M.R., Exploiting Macromolecular Design to Optimize the Antibacterial Activity of Alkylated Cationic Oligomers, Biomacromolecules, 2018, 19, 4629-4640 Gustafsson O.J.R., Briggs M.T., Condina M.R., Winderbaum L.J., Pelzing M., McColl S.R., Everest-Dass A.V., Packer N.H., Hoffmann P., Raw N-glycan mass spectrometry imaging data on formalin-fixed mouse kidney, Data in Brief, 2018, 21,185-188 Gustafsson O.J.R., Winderbaum L.J., Condina M.R., Boughton B.A., Hamilton B.R., Undheim E.A.B., Becker M., Hoffmann P., Balancing sufficiency and impact in reporting standards for mass spectrometry imaging experiments, GigaScience, 2018, 7 Han F., Soeriyadi A.H., Gooding J.J., Reversible Thermoresponsive Plasmonic Core-Satellite Nanostructures That Exhibit Both Expansion and Contraction (UCST and LCST), Macromolecular Rapid Communications, 2018, 39 Han F., Vivekchand S.R.C., Soeriyadi A.H., Zheng Y., Gooding J.J., Thermoresponsive plasmonic core-satellite nanostructures with reversible, temperature sensitive optical properties, Nanoscale, 2018, 10, 4284-4290 Han F.Y., Whittaker A., Howdle S.M., Naylor A., Shabir-Ahmed A., Smith M.T., Sustained-Release Hydromorphone Microparticles Produced by Supercritical Fluid Polymer Encapsulation, Journal of Pharmaceutical Sciences, 2018 Han F.Y., Whittaker A.K., Howdle S.M., Naylor A., Shabir-Ahmed A., Zhang C., Smith M.T., Formulation of bioerodible ketamine microparticles as an analgesic adjuvant treatment produced by supercritical fluid polymer encapsulation, Pharmaceutics, 2018, 10 Haque S., Boyd B.J., McIntosh M.P., Pouton C.W., Kaminskas L.M., Whittaker M., Suggested procedures for the reproducible synthesis of poly(D,l-lactide-coglycolide) nanoparticles using the emulsification solvent diffusion platform, Current Nanoscience, 2018, 14, 448-453 Haque S., Whittaker M., McIntosh M.P., Pouton C.W., Phipps S., Kaminskas L.M., A comparison of the lung

clearance kinetics of solid lipid nanoparticles and liposomes by following the 3H-labelled structural lipids after pulmonary delivery in rats, European Journal of Pharmaceutics and Biopharmaceutics, 2018, 125, 1-12 Hassan M., Martin A.D., Thordarson P., Engineering Biocompatible Scaffolds through the Design of Elastin-Based Short Peptides, ChemPlusChem, 2018, 83, 47-52 Healey R.D., Wojciechowski J.P., Monserrat-Martinez A., Tan S.L., Marquis C.P., Sierecki E., Gambin Y., Finch A.M., Thordarson P., Design, Synthesis, and Evaluation of N- and C-Terminal Protein Bioconjugates as G ProteinCoupled Receptor Agonists, Bioconjugate Chemistry, 2018, 29, 403-409 Huma Z.-E., Gupta A., Javed I., Das R., Hussain S.Z., Mumtaz S., Hussain I., Rotello V.M., Cationic silver nanoclusters as potent antimicrobials against multidrugresistant bacteria, ACS Omega, 2018, 3, 16721-16727 Ivask A., Pilkington E.H., Blin T., Käkinen A., Vija H., Visnapuu M., Quinn J.F., Whittaker M.R., Qiao R., Davis T.P., Ke P.C., Voelcker N.H., Uptake and transcytosis of functionalized superparamagnetic iron oxide nanoparticles in an: In vitro blood brain barrier model, Biomaterials Science, 2018, 6, 314-323 Javed I., Yu T., Peng G., SánchezFerrer A., Faridi A., Kakinen A., Zhao M., Mezzenga R., Davis T.P., Lin S., Ke P.C., In Vivo Mitigation of Amyloidogenesis through Functional-Pathogenic DoubleProtein Coronae, Nano Letters, 2018, 18, 5797-5804 Jia S., Fong W.-K., Graham B., Boyd B.J., Photoswitchable Molecules in Long-Wavelength Light-Responsive Drug Delivery: From Molecular Design to Applications, Chemistry of Materials, 2018, 30, 2873-2887 Jiang Z., Cheng H.-H., Blakey I., Whittaker A.K., Healing surface roughness of lithographic nanopatterns through sub-10 nm aqueous-dispersible polymeric particles with excellent dry etch durability, Molecular Systems Design and Engineering, 2018, 3, 627-635 Jiang Z., Pibaque Sanchez R.J., Blakey I., Whittaker A.K., 3D shape change of multiresponsive hydrogels based on a light-programmed gradient in volume phase transition,

Chemical Communications, 2018, 54, 10909-10912 Jimenez-Vargas N.N., Pattison L.A., Zhao P., Lieu T., Latorre R., Jensen D.D., Castro J., Aurelio L., Le G.T., Flynn B., Herenbrink C.K., Yeatman H.R., EdgingtonMitchell L., Porter C.J.H., Halls M.L., Canals M., Veldhuis N.A., Poole D.P., McLean P., Hicks G.A., Scheff N., Chen E., Bhattacharya A., Schmidt B.L., Brierley S.M., Vanner S.J., Bunnett N.W., Protease-activated receptor-2 in endosomes signals persistent pain of irritable bowel syndrome, Proceedings of the National Academy of Sciences of the United States of America, 2018, 115, E7438-E7447 Jin R., Liu Z., Bai Y., Zhou Y., Gooding J.J., Chen X., Core– Satellite Mesoporous Silica–Gold Nanotheranostics for Biological Stimuli Triggered Multimodal Cancer Therapy, Advanced Functional Materials, 2018, 28 Johnston S.T., Faria M., Crampin E.J., An analytical approach for quantifying the influence of nanoparticle polydispersity on cellular delivered dose, Journal of the Royal Society Interface, 2018, 15 Joyce P., Dening T.J., Meola T.R., Schultz H.B., Holm R., Thomas N., Prestidge C.A., Solidification to improve the biopharmaceutical performance of SEDDS: Opportunities and challenges, Advanced Drug Delivery Reviews, 2018 Joyce P., Gustafsson H., Prestidge C.A., Enhancing the lipase-mediated bioaccessibility of omega-3 fatty acids by microencapsulation of fish oil droplets within porous silica particles, Journal of Functional Foods, 2018, 47, 491-502 Joyce P., Gustafsson H., Prestidge C.A., Engineering intelligent particle-lipid composites that control lipasemediated digestion, Advances in Colloid and Interface Science, 2018, 260, 1-23 Joyce P., Prestidge C.A., Synergistic effect of PLGA nanoparticles and submicron triglyceride droplets in enhancing the intestinal solubilisation of a lipophilic weak base, European Journal of Pharmaceutical Sciences, 2018, 118, 40-48 Juno J.A., Waruk J.L.M., Wragg K.M., Mesa C., Lopez C., Bueti J., Kent S.J., Ball T.B., Kiazyk S.A., Mucosal-associated invariant T cells are depleted and exhibit altered chemokine

Kakinen A., Adamcik J., Wang B., Ge X., Mezzenga R., Davis T.P., Ding F., Ke P.C., Nanoscale inhibition of polymorphic and ambidextrous IAPP amyloid aggregation with small molecules, Nano Research, 2018, 11, 3636 - 3647 Kakinen A., Javed I., Faridi A., Davis T.P., Ke P.C., Serum albumin impedes the amyloid aggregation and hemolysis of human islet amyloid polypeptide and alpha synuclein, Biochimica et Biophysica Acta – Biomembranes, 2018, 1860, 1803-1809 Kakinen A., Sun Y., Javed I., Faridi A., Pilkington E.H., Faridi P., Purcell A.W., Zhou R., Ding F., Lin S., Ke P.C., Davis T.P., Physical and toxicological profiles of human IAPP amyloids and plaques, Science Bulletin, 2018 Kearnes M., Kuch D., Johnston A., How to do things with metaphors: Engineering life as hodgepodge Carmen McLeod, Brigitte Nerlich, Life Sciences, Society and Policy, 2018, 14 Kelly C.H.W., Benedetti T.M., Alinezhad A., Schuhmann W., Gooding J.J., Tilley R.D., Understanding the Effect of Au in Au-Pd Bimetallic Nanocrystals on the Electrocatalysis of the Methanol Oxidation Reaction, Journal of Physical Chemistry C, 2018, 122, 21718-21723 Kent S.J., Flexner C., Ageing in patients with chronic HIV infection: impact of hypercoagulation, AIDS Research and Therapy, 2018, 15, 22 Khan J., Rades T., Boyd B.J., Addition of Cationic Surfactants to Lipid-Based Formulations of Poorly Water-Soluble Acidic Drugs Alters the Phase Distribution and the Solid-State Form of the Precipitate Upon In Vitro Lipolysis, Journal of Pharmaceutical Sciences, 2018, 107, 2420-2427 Khiabani P.S., Kashi M.B., Zhang X., Pardehkhorram R., Markhali B.P., Soeriyadi A.H., Micolich A.P., Gooding J.J., A graphene-based sensor for real time monitoring of sun exposure, Carbon, 2018, 138, 215-218 Khor S.Y., Quinn J.F., Whittaker M.R., Truong N.P., Davis T.P., Controlling Nanomaterial Size and Shape for Biomedical Applications via Polymerization-

Induced Self-Assembly, Macromolecular Rapid Communications, 2018 Khor S.Y., Vu M.N., Pilkington E.H., Johnston A.P.R., Whittaker M.R., Quinn J.F., Truong N.P., Davis T.P., Elucidating the Influences of Size, Surface Chemistry, and Dynamic Flow on Cellular Association of Nanoparticles Made by Polymerization-Induced SelfAssembly, Small, 2018, 14 Koutsakos M., Wheatley A.K., Loh L., Clemens E.B., Sant S., Nüssing S., Fox A., Chung A.W., Laurie K.L., Hurt A.C., Rockman S., Lappas M., Loudovaris T., Mannering S.I., Westall G.P., Elliot M., Tangye S.G., Wakim L.M., Kent S.J., Nguyen T.H.O., Kedzierska K., Circulating TFH cells, serological memory, and tissue compartmentalization shape human influenza-specific B cell immunity, Science Translational Medicine, 2018, 10 Koziolek M., Carrière F., Porter C.J.H., Lipids in the Stomach – Implications for the Evaluation of Food Effects on Oral Drug Absorption, Pharmaceutical Research, 2018, 35 Kratochvil S., McKay P.F., Chung A.W., Kent S.J., Gilmour J., Shattock R.J., Corrigendum: Immunoglobulin G1 allotype influences antibody subclass distribution in response to HIV gp140 vaccination [Front Immunol, 8, 1883 (2017)] doi: 10.3389/fimmu.2017.01883, Frontiers in Immunology, 2018, 9 Kristensen A.B., Kent S.J., Parsons M.S., Contribution of NK cell education to both direct and anti-HIV-1 antibodydependent NK cell functions, Journal of Virology, 2018, 92 Larnaudie S.C., Sanchis J., Nguyen T.-H., Peltier R., Catrouillet S., Brendel J.C., Porter C.J.H., Jolliffe K.A., Perrier S., Cyclic peptidepoly(HPMA) nanotubes as drug delivery vectors: In vitro assessment, pharmacokinetics and biodistribution, Biomaterials, 2018, 178, 570-582 Ledo A.M., Sasso M.S., Bronte V., Marigo I., Boyd B.J., GarciaFuentes M., Alonso M.J., Codelivery of RNAi and chemokine by polyarginine nanocapsules enables the modulation of myeloid-derived suppressor cells, Journal of Controlled Release, 2019, 295, 60-73 Lee G.S., Pan Y., Scanlon M.J., Porter C.J.H., Nicolazzo J.A., Fatty Acid–Binding Protein 5 Mediates

the Uptake of Fatty Acids, but not Drugs, Into Human Brain Endothelial Cells, Journal of Pharmaceutical Sciences, 2018, 107, 1185-1193 Leong N.J., Mehta D., McLeod V.M., Kelly B.D., Pathak R., Owen D.J., Porter C.J.H., Kaminskas L.M., Doxorubicin Conjugation and Drug Linker Chemistry Alter the Intravenous and Pulmonary Pharmacokinetics of a PEGylated Generation 4 Polylysine Dendrimer in Rats, Journal of Pharmaceutical Sciences, 2018, 107, 2509-2513 Levrier C., Rockstroh A., Gabrielli B., Kavallaris M., Lehman M., Davis R.A., Sadowski M.C., Nelson C.C., Discovery of thalicthuberine as a novel antimitotic agent from nature that disrupts microtubule dynamics and induces apoptosis in prostate cancer cells, Cell Cycle, 2018, 17, 652-668 Li L., Zhang C., Zhang R., Xu Z., Xu Z., Whittaker A.K., , Multifunctional Magnetized Porous Silica Covered with Poly (2-dimethylaminoethyl methacrylate) for pH Controllable Drug Release and Magnetic Resonance Imaging, ACS Applied Nano Materials, 2018 Li T., Cipolla D., Rades T., Boyd B.J., Drug nanocrystallisation within liposomes, Journal of Controlled Release, 2018, 288, 96-110 Li T., Mudie S., Cipolla D., Rades T., Boyd B.J., Solid State Characterization of Ciprofloxacin Liposome Nanocrystals, Molecular Pharmaceutics, 2019 Liang F., Browne D.J., Gray M.J., Gartlan K.H., Smith D.D., Barnard R.T., Hill G.R., Corrie S.R., Markey K.A., Development of a Multiplexed Microsphere PCR for Culture-Free Detection and Gram-Typing of Bacteria in Human Blood Samples, ACS Infectious Diseases, 2018, 4, 837-844 Lin D.S., Kan A., Gao J., Crampin E.J., Hodgkin P.D., Naik S.H., DiSNE Movie Visualization and Assessment of Clonal Kinetics Reveal Multiple Trajectories of Dendritic Cell Development, Cell Reports, 2018, 22, 2557-2566 Lin Y., Genzer J., Li W., Qiao R., Dickey M.D., Tang S.-Y., Sonication-enabled rapid production of stable liquid metal nanoparticles grafted with poly(1-octadecene-: Alt -maleic anhydride) in aqueous solutions, Nanoscale, 2018, 10, 1987119878

Liu Y., Zhang C., Liu H., Li Y., Xu Z., Li L., Whittaker A., Controllable synthesis of up-conversion nanoparticles UCNPs@MIL-PEG for pH-responsive drug delivery and potential up-conversion luminescence/magnetic resonance dual-mode imaging, Journal of Alloys and Compounds, 2018, 749, 939-947 Liu Y., Zhang C., Xu C., Lin C., Sun K., Wang J., Chen X., Li L., Whittaker A.K., Xu H.-B., Controlled synthesis of up-conversion luminescent Gd/ Tm-MOFs for pH-responsive drug delivery and UCL/MRI dual-modal imaging, Dalton Transactions, 2018, 47, 11253-11263 Lopez E., Shattock R.J., Kent S.J., Chung A.W., The Multifaceted Nature of Immunoglobulin A and Its Complex Role in HIV, AIDS Research and Human Retroviruses, 2018, 34, 727-738 Lu X., Nicovich P.R., Zhao M., Nieves D.J., Mollazade M., Vivekchand S.R.C., Gaus K., Gooding J.J., Monolayer surface chemistry enables 2-colour single molecule localisation microscopy of adhesive ligands and adhesion proteins, Nature Communications, 2018, 9 Ly J., Li Y., Vu M.N., Moffat B.A., Jack K.S., Quinn J.F., Whittaker M.R., Davis T.P., Nanoassemblies of cationic mPEG brush block copolymers with gadolinium polyoxotungstate [Gd(W5O18)2]9- form stable, high relaxivity MRI contrast agents, Nanoscale, 2018, 10, 7270-7280 Ma Y., Björnmalm M., Wise A.K., Cortez-Jugo C., Revalor E., Ju Y., Feeney O.M., Richardson R.T., Hanssen E., Shepherd R.K., Porter C.J.H., Caruso F., GelMediated Electrospray Assembly of Silica Supraparticles for Sustained Drug Delivery, ACS Applied Materials and Interfaces, 2018, 10, 31019-31031 Mahmoud A.M., Rajakanthan A., Kempe K., Functional hydrophobic and hetero-grafted block comb polymers: Via a combination of spontaneous zwitterionic copolymerisation and redox-initiated RAFT polymerisation, Polymer Chemistry, 2018, 9, 1562-1566 Martin A.D., Chua S.W., Au C.G., Stefen H., Przybyla M., Lin Y., Bertz J., Thordarson P., Fath T., Ke Y.D., Ittner L.M., Peptide Nanofiber Substrates for Long-Term Culturing of Primary Neurons, ACS Applied Materials and Interfaces, 2018, 10, 25127 -25134 CBNS Annual Report 2018 81

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receptor expression and elevated granulocyte macrophage-colony stimulating factor production during end-stage renal disease, Frontiers in Immunology, 2018, 9

Mehta D., Leong N., McLeod V.M., Kelly B.D., Pathak R., Owen D.J., Porter C.J.H., Kaminskas L.M., Reducing Dendrimer Generation and PEG Chain Length Increases Drug Release and Promotes Anticancer Activity of PEGylated Polylysine Dendrimers Conjugated with Doxorubicin via a Cathepsin-Cleavable Peptide Linker, Molecular Pharmaceutics, 2018, 15, 4568-4576 Meola T.R., Dening T.J., Prestidge C.A., Nanocrystalsilica-lipid hybrid particles for the improved oral delivery of ziprasidone in vitro, European Journal of Pharmaceutics and Biopharmaceutics, 2018, 129, 145-153 Montagnat O.D., Webster G.R., Bulitta J.B., Landersdorfer C., Wyber R., Sheel M., Carapetis J.R., Boyd B.J., Lessons learned in the development of sustained release penicillin drug delivery systems for the prophylactic treatment of rheumatic heart disease (RHD), Drug Delivery and Translational Research, 2018, 8, 729-739 Moonshi S.S., Zhang C., Peng H., Puttick S., Rose S., Fisk N.M., Bhakoo K., Stringer B.W., Qiao G.G., Gurr P.A., Whittaker A.K., A unique 19F MRI agent for the tracking of non phagocytic cells: In vivo, Nanoscale, 2018, 10, 8226-8239 Nadiminti P.P., Liu Q., Vanjari L.K., Dong Y.D., Boyd B.J., Cahill D.M., Novel self-assembling conjugates as vectors for agrochemical delivery, Journal of Nanobiotechnology, 2018, 16, 94 Nicolas J., Liu S., Zhao D., Caruso F., Reichmanis E., Buriak J.M., Best Practices for New Polymers and Nanoparticulate Systems, Chemistry of Materials, 2018, 30, 6587-6588 Oliver S., Yee E., Kavallaris M., Vittorio O., Boyer C., Water Soluble Antioxidant Dextran– Quercetin Conjugate with Potential Anticancer Properties, Macromolecular Bioscience, 2018, 18 Önal E., Zhang C., Davarcı D., İşci Ü., Pilet G., Whittaker A.K., Dumoulin F., Cyclotriphosphazene, a scaffold for 19F MRI contrast agents, Tetrahedron Letters, 2018, 59, 521-523 Pan M., Gawthrop P.J., Tran K., Cursons J., Crampin E.J., A thermodynamic framework for modelling membrane transporters, Journal of Theoretical Biology, 2018

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Pan M., Gawthrop P.J., Tran K., Cursons J., Crampin E.J., Bond graph modelling of the cardiac action potential: Implications for drift and non-unique steady states, Proceedings of the Royal Society A: Mathematical, Physical and Engineering Sciences, 2018, 474 Pan S., Guo R., Björnmalm M., Richardson J.J., Li L., Peng C., Bertleff-Zieschang N., Xu W., Jiang J., Caruso F., Coatings super-repellent to ultralow surface tension liquids, Nature Materials, 2018, 17, 1040-1047 Pan Y., Morris E.R., Scanlon M.J., Marriott P.J., Porter C.J.H., Nicolazzo J.A., Dietary docosahexaenoic acid supplementation enhances expression of fatty acidbinding protein 5 at the blood–brain barrier and brain docosahexaenoic acid levels, Journal of Neurochemistry, 2018, 146, 186-197 Parker S.G., Yang Y., Ciampi S., Gupta B., Kimpton K., Mansfeld F.M., Kavallaris M., Gaus K., Gooding J.J., A photoelectrochemical platform for the capture and release of rare single cells, Nature Communications, 2018, 9 Parmar A., Pascali G., Voli F., Lerra L., Yee E., Ahmed-Cox A., Kimpton K., Cirillo G., Arthur A., Zahra D., Rahardjo G., Liu G.J., Lengkeek N., Saletta F., Charil A., Kavallaris M., Vittorio O., In vivo [64Cu]CuCl2 PET imaging reveals activity of dextranCatechin on tumor copper homeostasis, Theranostics, 2018, 8, 5645-5659 Parsons M.S., Chung A.W., Kent S.J., Importance of Fcmediated functions of anti-HIV-1 broadly neutralizing antibodies, Retrovirology, 2018, 15 Parsons M.S., Cromer D., Davenport M.P., Kent S.J., HIV Reactivation after Partial Protection by Neutralizing Antibodies, Trends in Immunology, 2018, 39, 359-366 Parsons M.S., Le Grand R., Kent S.J., Neutralizing antibodybased prevention of cellassociated HIV-1 infection, Viruses, 2018, 10 Pei Y., Shahoei S.H., Li Y., Reece P.J., Nelson E.R., Gooding J.J., Kilian K.A., Vertical Integration of Cell-Laden Hydrogels with Bioinspired Photonic Crystal Membranes, Advanced Materials Interfaces, 2018, 5 Peng Y., Lin D., Gooding J.J., Xue Y., Dai L., Flexible fiber-shaped

non-enzymatic sensors with a graphene-metal heterostructure based on graphene fibres decorated with gold nanosheets, Carbon, 2018, 136, 329-336 Pilkington E.H., Gustafsson O.J.R., Xing Y., HernandezFernaud J., Zampronio C., Kakinen A., Faridi A., Ding F., Wilson P., Ke P.C., Davis T.P., Profiling the Serum Protein Corona of Fibrillar Human Islet Amyloid Polypeptide, ACS Nano, 2018, 12, 6066-6078 Qiao R., Esser L., Fu C., Zhang C., Hu J., Ramírez-Arcía P., Li Y., Quinn J.F., Whittaker M.R., Whittaker A.K., Davis T.P., Bioconjugation and Fluorescence Labeling of Iron Oxide Nanoparticles Grafted with Bromomaleimide-Terminal Polymers, Biomacromolecules, 2018, 19, 4423-4429 Rahim M.A., Björnmalm M., Bertleff-Zieschang N., Ju Y., Mettu S., Leeming M.G., Caruso F., Multiligand Metal-Phenolic Assembly from Green Tea Infusions, ACS Applied Materials and Interfaces, 2018, 10, 7632-7639 Rahim M.A., Hata Y., Björnmalm M., Ju Y., Caruso F., Supramolecular Metal–Phenolic Gels for the Crystallization of Active Pharmaceutical Ingredients, Small, 2018, 14 Rahim M.A., Kristufek S.L., Pan S., Richardson J.J., Caruso F., Phenolic Building Blocks for the Assembly of Functional Materials, Angewandte Chemie – International Edition, 2018 Rajagopal V., Bass G., Ghosh S., Hunt H., Walker C., Hanssen E., Crampin E., Soeller C., Creating a structurally realistic finite element geometric model of a cardiomyocyte to study the role of cellular architecture in cardiomyocyte systems biology, Journal of Visualized Experiments, 2018, 2018 Ramarathinam S.H., Gras S., Alcantara S., Yeung A.W.S., Mifsud N.A., Sonza S., Illing P.T., Glaros E.N., Center R.J., Thomas S.R., Kent S.J., Ternette N., Purcell D.F.J., Rossjohn J., Purcell A.W., Identification of Native and Posttranslationally Modified HLA-B*57:01-Restricted HIV Envelope Derived Epitopes Using Immunoproteomics, Proteomics, 2018, 18 Ramesan S., Rezk A.R., Dekiwadia C., Cortez-Jugo C., Yeo L.Y., Acoustically-mediated intracellular delivery, Nanoscale, 2018, 10, 13165-13178

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Silva S.M., Hoque S., Gonçales V.R., Gooding J.J., The Impact of the Position of the Redox Label on Charge Transfer and Hybridization Efficiency at DNA Interfaces, Electroanalysis, 2018, 30, 1529-1535 Silva S.M., Tavallaie R., Gonçales V.R., Utama R.H., Kashi M.B., Hibbert D.B., Tilley R.D., Gooding J.J., Dual Signaling DNA Electrochemistry: An Approach to Understand DNA Interfaces, Langmuir, 2018, 34, 1249-1255 Sohail M.F., Hussain S.Z., Saeed H., Javed I., Sarwar H.S., Nadhman A., Huma Z.-E., Rehman M., Jahan S., Hussain I., Shahnaz G., Polymeric nanocapsules embedded with ultra-small silver nanoclusters for synergistic pharmacology and improved oral delivery of Docetaxel, Scientific Reports, 2018, 8 Sriram M., Markhali B.P., Nicovich P.R., Bennett D.T., Reece P.J., Brynn Hibbert D., Tilley R.D., Gaus K., Vivekchand S.R.C., Gooding J.J., A rapid readout for many single plasmonic nanoparticles using darkfield microscopy and digital color analysis, Biosensors and Bioelectronics, 2018, 117, 530-536 Steinkoenig J., De Jongh P.A.J.M., Haddleton D.M., Goldmann A.S., Barner-Kowollik C., Kempe K., Unraveling the Spontaneous Zwitterionic Copolymerization Mechanism of Cyclic Imino Ethers and Acrylic Acid, Macromolecules, 2018, 51, 318-327 Su Y.-W., Chim S.M., Zhou L., Hassanshahi M., Chung R., Fan C., Song Y., Foster B.K., Prestidge C.A., Peymanfar Y., Tang Q., Butler L.M., Gronthos S., Chen D., Xie Y., Chen L., Zhou X.-F., Xu J., Xian C.J., Osteoblast derived-neurotrophin‑3 induces cartilage removal proteases and osteoclast-mediated function at injured growth plate in rats, Bone, 2018, 116, 232-247 Suys E.J.A., Chalmers D.K., Pouton C.W., Porter C.J.H., Polymeric Precipitation Inhibitors Promote Fenofibrate Supersaturation and Enhance Drug Absorption from a Type IV Lipid-Based Formulation, Molecular Pharmaceutics, 2018, 15, 2355-2371

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Zhao Q., Yang D., Zhang C., Liu X.-H., Fan X., Whittaker A.K., Zhao X.S., Tailored PolyimideGraphene Nanocomposite as Negative Electrode and Reduced Graphene Oxide as Positive Electrode for Flexible Hybrid Sodium-Ion Capacitors, ACS Applied Materials and Interfaces, 2018, 10, 43730-43739 Zhao Y.i, Fletcher N. L., Liu T., Gemmell A.C., Houston Z.H., Blakey I., Thurecht K. J., In vivo therapeutic evaluation of polymeric nanomedicines: effect of different targeting peptides on therapeutic efficacy against breast cancer, Nanotheranostics, 2018, 2, 360-370 Zhong Q.-Z., Pan S., Rahim M.A., Yun G., Li J., Ju Y., Lin Z., Han Y., Ma Y., Richardson J.J., Caruso F., Spray Assembly of MetalPhenolic Networks: Formation, Growth, and Applications, ACS Applied Materials and Interfaces, 2018, 10, 33721-33729 Zhu M., Whittaker A.K., Smith M.T., Han F.Y., Bioerodable Ketamine-Loaded Microparticles Fabricated Using Dissolvable Hydrogel Template Technology, Journal of Pharmaceutical Sciences, 2019, 108, 12201226

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