CBNS 2019 Annual Report by SCDesign

ANNUAL REPORT 2019

Collaborating Organisations

Partner Organisations

Feature photography of CBNS staff, students and laboratories throughout this report by Joe Vittorio and Ava Faridi. Other photographs and images courtesy of CBNS members and organisations, unless otherwise attributed. Content compiled and edited by Samantha Snowden-Finch. Graphic design by Sophie Campbell, SCDesign.

AB O UT C B N S About CBNS

Directorâ&#x20AC;&#x2122;s Report

CBNS at a Glance

Strategic Plan

Research

Research Overview

Signature Projects

Overarching Projects

Cross-Centre Activity

Strategic Projects

R E S EAR C H

Contents

CBNS Experience

Events

Education and Training Committee

Industry Partners Program Committee

Outreach Sub-Committee

CBNS Awards

CBNS Podcasts

Media Coverage

Governance

Governance and Management

Governance Board

Scientific Advisory Board

Performance

Performance and KPIs

Financial Report 2019

G OVE R NAN C E

Collaborations and Partnerships

E N GAG E M E NT

Engagement 35

CBNS Personnel

Visitors to CBNS

Publications 78

CBNS Annual Report 2019 1

P E R FO R MAN C E

Awards, Memberships and Grant Success 67

About CBNS The Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology or, as we prefer to be known, ARC Centre for Bio-Nano Science (CBNS) was established in mid-2014 as a national innovator in bio-nano sciences. We bring together a diverse team of Australiaâ&#x20AC;&#x2122;s leading scientists with the aim of developing next-generation bio-responsive nano-structured materials. The key scientific aim that underpins all the activities of the Centre is to fully understand and then exploit, the interface between nano-engineered materials and biological systems. The CBNS research program is structured around the applications of understanding this interface: drug and gene delivery; vaccines; bio-imaging â&#x20AC;&#x201C; both cellular and whole body imaging; and sensors and diagnostics. CBNS research is integrated by overarching research activities to understand the social dimensions of bio-nanotechnology, to visualise bio-nano interactions, and by using a systems biology approach to fully

Health and biomedical sector engagement

describe the complex interactions that dictate success or failure of nanotechnology for therapeutic applications. In delivering the promise of bio-nano science, CBNS has brought together Australiaâ&#x20AC;&#x2122;s leading scientists and engineers with expertise in nanotechnology, polymer science, cell biology, cancer biology, systems biology, chemical engineering, 3D CGI, immunology, chemistry and social science. The Centre consists of five collaborating organisations (nodes): Monash University (Monash), the University of Melbourne (UoM), the University of Queensland (UQ),

Centre for Bio-Nano Science

the University of New South Wales (UNSW) and the University of South Australia (UniSA). In addition, there are nine overseas partners and the Australian Nuclear Science and Technology Organisation. CBNS is a seven-year program of research and is funded by the ARC ($26M) and the Australian collaborating universities ($9M) over this period. The universities also contribute in-kind, as do the partner organisations. In total the in-kind support for CBNS is valued at more than $23M over the life of the program and we thank all of the contributors for this support.

Education and training

Delivery (delivery systems, vaccines)

Computational biology

Understanding and exploiting the bio-nano interface

Social dimensions

Detection (sensors and diagnostics, imaging technologies)

Community outreach

2 CBNS Annual Report 2019

Visualisation

Industry and commercial engagement

AB O UT C B N S CBNS Annual Report 2019 3

Director’s Report Collaboration was crucial to a very successful 2019 year for CBNS as members continued to work on cutting-edge science related to the interfaces of nano-engineered materials and biological systems. With over 360 members undertaking innovative research on 10 projects, we showcased excellence with 226 publications; 14 outreach events; and 30 completed postgraduate studies.

We saw the successful implementation of the 2018 – 2020 Strategic Plan and our collaborating and partner universities worked collegially to translate their nano research into better methods, tools and technologies. CBNS continues to be a global leader in bio-nano science discovery research with 35 international lab visits, 46 visitors including the 2019 Visiting Professor Chunying Chen; a Professor at The National Center for Nanoscience and Technology in China. Professor Chen delivered a plenary talk at the 10th International Nanomedicine Conference in June and provided an overview of nanomedicine research in China at the CBNS Annual Research Workshop (ARW) in November. This engagement opened up a number of in-depth discussions and opportunities for future collaboration with CBNS research teams.

CBNS continues to be a global leader in bio-nano science discovery research with 35 international lab/other visits and 46 visitors. Excellence was evident through CBNS members receiving many awards, grants and prizes. A few particular prestigious awards include Chief Investigator (CI) Professor Maria Kavallaris AM being appointed* a Member of the Order of Australia Medal and receiving a Lemberg Medal from the Australian Society for Biochemistry and Molecular Biology; Deputy Director Professor Frank Caruso gained international recognition with The Leverhulme Medal from The Royal Society. Postdoctoral Researcher

4 CBNS Annual Report 2019

Dr Marnie Winter and PhD candidate Ms Aria Ahmed-Cox were also recipients of international awards. Marnie received a considerable grant from The Bill and Melinda Gates Foundation and Aria was selected to be part of a small cohort of women from across the world to develop leadership skills that will benefit the greater good. 2019 saw the development of a new Strategic Project, The Neuro-Nano interface – Using nanotechnology to provide a window into pain. The two-year project aims to bridge the gap between neuroscience and nanotechnology, and provide new insights and new outreach/education opportunities through visualisation of pain states and associated discussion about the potential for nanotechnology to change drug targeting and drug properties for pain. The Strategic Project highlights the research expertise across nodes (Monash, UQ and UNSW), and involves CBNS researchers; Dr Nicholas

UNSW Primary School Science Fete

Veldhuis, Dr Daniel Poole, Dr Paulina Ramírez García, Dr Michael Whittaker, Professor Kris Thurecht, Associate Professor Matthew Kearnes and Associate Professor John McGhee. Leveraging the success of the Centre’s Education and Training Program, in 2019 CBNS refined the program to create three distinct streams to assist members in their professional development. The Centre delivered workshops that were aligned with Science Training (technical skills related to scientific research); Career Development (soft skills designed to motivate and promote career growth); and Skills Development (promoting key skills such as communication, media engagement and time management). Engaging with the general public, National Science Week, held in August, was a huge success for CBNS with all node cities participating in outreach events. Collectively our reach was over 6500 people.

AB O UT C B N S Monash and UoM started the week with Extrasensory in Melbourne where we demonstrated some of the outcomes of our Strategic Project, Journey to the Centre of The Cell (JTCC) with virtual reality (VR) experiences for attendees. UNSW played host to a primary school science fete and our VR experience was again a major hit. South Australian secondary school students were invited to explore the bio-nano world through hands-on lab experiments, VR, games and talks at UniSA. We concluded the CBNS outreach activities with postgraduate students and postdoctoral researchers from UQ, participating in a weekend event at the Brisbane Science Festival.

In 2019, our members were supported and nurtured through several Centre-led initiatives that enabled them to focus on developing their professional networks. Our achieved objective was to introduce the bio-nano realm to the general public and to further develop members experience with communicating their research.

We launched the CBNS Bio-Nano Travel Grant to provide financial support to postdoctoral researchers across our nodes and continued to offer the CBNS Student Travel Award and the CBNS Carer’s Support Fund. Five awards (one for each CBNS node) for both the Bio-Nano Travel Grant and the Student Travel Award were issued, with each recipient invited to the CBNS ARW to present on their opportunity. The Carer’s Support Fund had four successful applicants who used the funds to assist with their extraordinary carer’s costs associated with being a primary carer, enabling them to attend a conference or meeting that would further the development of their research careers. The introduction of the Industry Partners Program (IPP) to strengthen CBNS research connections with industry, was well received by Centre members and stakeholders. The IPP Committee, chaired by Professor Kris Thurecht, met three times during 2019 and organised a successful Industry Workshop in November, where key industry partners and CBNS alumni were invited to talk about their experiences moving from bench research to industry and progressing through to senior leadership and management roles. A key objective

for the Committee was to develop and launch a Translation Strategy to support the research undertaken by Centre members and outline the processes required to take their ideas to industry/ market. The Translation Strategy was supplemented with the creation of a CBNS Toolkit, a series of resources available at each of the nodes, to facilitate the development of specific research ideas. While some of our staff have moved on to other roles and we thank them for their contribution, we have been fortunate to welcome some new outstanding staff to the Centre. This includes our new Senior Centre Administrator, Cassie Statkevitch as well as Senior Events and Communications Coordinator, Samantha Snowden-Finch (maternity leave replacement for Anne Meyer). We look forward to strengthening the CBNS legacy across our research, engagement and collaboration in the final phase of Centre operations.

Professor Tom Davis Centre Director

CBNS Annual Report 2019 5

CBNS at a Glance Timeline 68 months into 84 month program 2014 2015 2016 2017 2018 2019 2020 2021

Funding $9M in University funding

Partners and collaborators

$35M

over 7 years

$26M in ARC funding

Australian universities

partner organisations

Key data

49 publications in journals with impact factors >10

Our people

Chief Investigators

talks given

new fellowships awarded

18 Senior research fellows undertaking CBNS research

Post-doctoral researchers

360 people

Honours and Masters students

6 Management, administration and operational staff

6 CBNS Annual Report 2019

Diverse disciplines: bioimaging, cell biology, chemistry, engineering, immunology, pharmacology, sociology, systems biology, visual arts

Partner Investigators

163

Research assistants and technical staff

PhD students

US Partner Organisations: Columbia University Medical Center Memorial Sloan Kettering Cancer Center (New York) University of California, Santa Barbara University of Wisconsin-Madison

European Partner Organisations: University of Nottingham University of Warwick Imperial College London University College Dublin

Asian Partner Organisation: Sungkyunkwan University, Korea

Australian Partner Organisation: ANSTO (Sydney)

Collaborating Organisations

University of Queensland

University of New South Wales University of South Australia Monash University (Administering Organisation) University of Melbourne

CBNS Annual Report 2019 7

AB O UT C B N S

Partner Organisations

Strategic Plan We reviewed our Strategic Plan to provide a framework from which the CBNS can deliver world class, innovative research in bio-nano science. This work was done through a broad consultation process with members of the CBNS and stakeholders. Through this Strategic Plan, the Centre will leave a legacy of research excellence and innovation, together with a highly skilled and professional workforce, ready to lead bio-nano science research into the future. Below is a summary of the Strategic Plan. A detailed version can be found on our website.

Our Vision

Our Values

The CBNS envisions a world where new technologies are used to understand, treat and diagnose conditions with nanoscale precision.

Excellence The CBNS is a global leader in bio-nano science discovery research and innovation, delivering excellence across the scientific endeavour.

Our Mission The CBNS will conduct cutting-edge research and discovery science to understand the interface between nano-engineered materials and biological systems, and translate these discoveries into better methods, tools and technologies.

8 CBNS Annual Report 2019

Belonging The CBNS welcomes and embraces all members, perspectives, ideas and experiences; fostering an environment with equal opportunity for all. Nurture The CBNS is a place where new ideas are nourished, and members are inspired, motivated and empowered to move towards their personal career goals.

Collaboration The CBNS promotes team approaches that capitalise on the multidisciplinary expertise of its members to innovate, imagine and lead research in bio-nano science and technology. Integrity Responsible, respectful, honest, ethical and professional conduct underlies all CBNS research activities, leadership and operations.

AB O UT C B N S

Our Priorities 1. Conduct world-class transformative research that is innovative, collaborative and informed by the multi disciplinary expertise of CBNS members and their strong national and international networks. 2. Inspire, train and nurture the next generation of Australian leaders in science and technology. 3. Strengthen connections with stakeholders, industry and end-users. 4. Connect CBNS to the public. 5. Foster a diverse, equal-opportunity and family-friendly workplace.

CBNS Annual Report 2019 9

Organisational Structure A revision of the governance and organisational structure of CBNS has led to the addition of an Industry Partners Program. This change was made to ensure appropriate oversight was provided to meet the strategic priorities focused on increasing industry and stakeholder engagement. All other governance and organisational structures remain in place.

Executive Committee

Scientific Advisory Board

Governance Board

Director: Professor Tom Davis Deputy Director: Professor Frank Caruso

Chair: Professor Alan Rowan Centre Manager Dr Natalie Jones

Chair Education and Training Program

Chair Outreach Program

Chair: Professor Peter Doherty AC Chair Industry Partners Program

Centre Administration

Education and Training Program Chair Professor Maria Kavallaris AM

Monash node

UoM node

UQ node

UNSW node

UniSA node

Node Leader Professor Tom Davis

Node Leader Professor Frank Caruso

Node Leader Professor Andrew Whittaker

Node Leader Professor Justin Gooding

Node Leader Professor Benjamin Thierry

Outreach Program Chair Associate Professor Matthew Kearnes

Industry Partners Program Chair Professor Kris Thurecht

Research Program Research Themes Delivery Systems and Vaccines

Sensors and Diagnostics

Imaging and Visualisation

Overarching Projects/Signature Projects Chief Investigators, Co-lead Investigators, Postdoctoral Researchers, Research Staff, Research Students

10 CBNS Annual Report 2019

Strategic Fund

AB O UT C B N S

Strategic Priorities and Goals PRIORITIES

GOALS

Conduct world-class transformative research that is innovative, collaborative and informed by the multi-disciplinary expertise of CBNS members and their strong national and international networks.

Inspire, train and nurture the next generation of Australian leaders in science and technology

To be a place where research leaders are created and armed with the skills and network they need to meet future challenges.

Strengthen connections with stakeholders, industry and end-users

Connect CBNS to the public

To be a conduit for information transfer between the scientific community and the public, increasing awareness, understanding and acceptance of bio-nano science and technology.

Foster a diverse, equal-opportunity and family-friendly workplace

To be an environment that reflects and embraces diversity, provides equal opportunity for all, and is family-friendly, so all members feel welcome, supported and safe.

To facilitate new opportunities for CBNS collaboration and create processes for increased sharing of knowledge, expertise and ideas across the Centre.

CBNS Annual Report 2019 11

Goals and Initiatives

To better predict, control and visualise the myriad interactions that occur between nanomaterials and the biological environment, and translate these research discoveries into better methods, tools and technologies • Establish bio-nano standards for international use • Develop new imaging technologies, assays, tools and capabilities to understand the nano-engineered material and biological interface, including 3D bioprinting, multicellular organoids/spheroid models, lab-on-chip and microfluidics • Use virtual reality technologies to explore and enhance society’s understanding of new bio-nanoscale technologies

To be a place where research leaders are created and armed with the skills and network they need to meet future challenges. • Develop a centrally coordinated career pathways and professional development program for CBNS members, that recognises the diverse career paths open to CBNS staff and students • Develop PhD and EMCR networks at each node, and a PhD and EMCR CBNS committee, comprised of the leaders of each network from each node • Continue to support the Education and Training program to provide members with ongoing scientific skills and professional training • Improve and coordinate communication across CBNS to ensure all members have timely and equitable access to information, opportunities and resources regardless of their geographical location • Establish pathways for succession planning

• Promote collaboration and utilise the multi-disciplinary expertise that exists across CBNS • Encourage discovery, by supporting and testing new ideas and new ways of thinking about bio-nano science and technology

To facilitate new opportunities for CBNS collaboration and create processes for increased sharing of knowledge, expertise and ideas across CBNS. • Create time for both professional and social networking at CBNS events • Promote the use of videoconferencing as a platform for regular exchange of knowledge, ideas and information between members located at different sites and nodes • Increase awareness of CBNS exchange programs that support placement of CBNS members to work in laboratories of other CBNS members

12 CBNS Annual Report 2019

To increase engagement with stakeholders, industry partners and other relevant end-users, to support ongoing research activities, promote translation of research findings and ensure the research directions of CBNS respond to the changing social and political environment. • Build the profile of CBNS to ensure its reputation as a national focal point of research excellence in bio-nano science is recognised by other scientists, industry and end-users. This will create new opportunities for continued scientific endeavours, including implementation and uptake of research outcomes • Develop an Industry Partners Program, to coordinate industry engagement activities, build networks, expand mentoring program and support ongoing relationships and communication with industry and end-users. This program will: – Develop formal partnership events, to promote CBNS research to industry and end-users – Create a translation strategy, with advice from broader industry and end-user groups, including health services and agricultural scientists, to create a pipeline from knowledge creation to implementation and impact

AB O UT C B N S To be a conduit for information transfer between the scientific community and the public, increasing awareness, understanding and acceptance of bio-nano science and technology. • Continue to support and build on the current Outreach Program to communicate information about bio-nano science and technology to the public, through primary and secondary schools, public events, national science week and other university and CBNS initiatives • Evaluate and increase social readiness for bio-nano science and technology innovations, to ensure research outcomes are attractive, understood and suitable for the public • Build the reputation of CBNS as the focal point of bio-nano science knowledge and expertise in Australia and facilitate bi-directional communication between the scientific community and the public

To be an environment that reflects and embraces diversity, provides equal opportunity for all and is family-friendly, so all members feel welcome, supported and safe. • Raise awareness and regularly review the diversity, gender equality and family-friendly practices within CBNS policy, to ensure it supports the creation of an inclusive, equitable and family friendly culture • Promote opportunities for career development for all members, to demonstrate our support and commitment to inclusion across sex, race, gender, age, religion, identity and experience. This includes participation in the mentoring program, and on internal and external committees, providing opportunities to present at conferences, workshops and forums, representing CBNS at scientific societies, participating in advocacy roles and in the media and public events • Seek ongoing feedback and suggestions to increase support for those with families and care responsibilities to attend internal CBNS and external events

Research Goals

Delivery Systems and Vaccines • To facilitate a priori design of nanomaterial-based delivery systems that can 1) target specific organ and cell types and 2) deliver therapeutically active cargoes to precise cellular and intracellular destinations. • To understand interactions of nanomaterials with the immune system to enable nanoengineered materials to improve vaccine design and efficacy.

Sensors and Diagnostics

Imaging and Visualisation

• To be able to detect and quantify, with minimal sample preparation and without calibration or amplification, rare biomolecular entities directly in biological fluids.

• To lead the field of the science of imaging and visualisation by addressing the three main elements of the imaging “pipeline”: 1) image acquisition, 2) simulation & modelling and 3) visualisation.

• To be able to detect, characterise and manipulate rare mammalian and bacterial cells in whole blood.

• To provide clear teaching of all aspects of synthesis of high-performing and responsive imaging agents, image acquisition and interpretation of results (including development of physics-based models) and visualisation.

CBNS Annual Report 2019 13

14 CBNS Annual Report 2019

R E S EAR C H

Research CBNS Annual Report 2019 15

16 CBNS Annual Report 2019

Research Overview In line with the updated Strategic Plan, the CBNS research structure focuses on four elements: the engineering of nanomaterials; the study of the interaction between these materials and the biological systems; the stimulation on immune responses; as well as the reporting of biological events. The revised structure includes three research themes that underpin the research program:

2 Imaging and Visualisation – led by Professor Andrew Whittaker (UQ) 3 Sensors and Diagnostics – led by Professor Justin Gooding (UNSW) Each theme has one or more signature project/s that will work towards achieving the research goals. Additionally, four overarching projects have been developed which span the research themes. Together, these projects illustrate the coordinated approach and complementary research conducted across the Centre and will ensure that fundamental discoveries in bio-nano science and technology enable a range of end-user applications. The signature projects capture the ultimate application of collaboration within the Centre’s research: every CBNS researcher engages in at least one of these signature projects embracing the collaborative spirit of the Centre.

OVERARCHING PROJECTS Social dimensions of bio-nano interactions (page 24)

Predicting bio-nano interactions (page 25)

Development of complex cellular systems for the evaluation and characterisation of bio-nano interactions (page 26)

Improved molecular imaging agents (page 27)

Research themes:

Delivery Systems & Vaccines

Sensors & Diagnostics

Imaging & Visualisation

Signature projects:

A material scientist’s guide to the cell (page 18)

New tools for quantifying and manipulating rare entities directly in biological fluids (page 21)

JTCC: Visualising bio-imaging and data in 3D virtual reality (page 22)

Overcoming challenges of in-vivo targeting (page 19) Mediating protein interactions (page 20)

The research program and expertise within CBNS is presented in more detail in the following pages.

CBNS Annual Report 2019 17

R E S EAR C H

1 Delivery Systems and Vaccines – led by Professor Chris Porter (Monash) and Professor Stephen Kent (UoM)

Signature Projects DELIVERY SYSTEMS & VACCINES A material scientist’s guide to the cell Leaders: Dr Angus Johnston, Professor Rob Parton Co-Leaders: Dr Nicholas Veldhuis, Dr Laura Fitzgerald Collaborating organisations/groups: Professor Justin Gooding (UNSW), Professor Nigel Bunnett (Columbia University Medical Center), Professor Cameron Alexander (University of Nottingham), Professor Marino Zerial (Max Planck Institute, Dresden), Professor Satyajit Mayor (National Centre for Biological Science Bangalore), Dr Georgina Such (UoM)

The project

Outcome/s

Effective drug delivery depends on delivering drugs to the sites in the cell where they are therapeutically active. Nanoparticles are an attractive strategy for delivering agents in a controlled and precise manner. The aim of this project is to gain a fundamental understanding of how nanoparticles are trafficked in cells. We are focusing on understanding how nanoparticles and their cargo are internalised, trafficked in the endosomes and then move from the endosome into the cytoplasm (i.e. endosomal escape). These fundamental interactions are poorly understood and by understanding these interactions, we aim to engineer the next generation of nanoparticles that can respond intelligently to the cellular environment.

Understanding how endosomal escape occurs and optimising endosomal escape to improve the efficacy of nanomedicines.

Value This work is crucial for achieving nanoparticle delivery, with universal applications in nanomedicine.

Activities undertaken in 2019 Endosomal escape in cells: • Testing penetrating peptides as endosomal escape agents. • Critical analysis of the proton sponge effect.

Activities planned for 2020 • Review the current understanding in endocytosis mechanisms and techniques to determine which pathways are involved for a given material. • Collaborating with the 3DVAL team on the Nanoscapes project.

Nanoparticles breaking out of endosomes. Image: Cover from Bioconjugate Chemistry. American Chemical Society, 30(2), 2019 by Laura Fitzgerald

18 CBNS Annual Report 2019

Overcoming challenges of in vivo targeting Leaders: Professor Kris Thurecht, Professor Frank Caruso Co-Leaders: Dr Zach Houston, Dr Matt Faria

The project

Activities undertaken in 2019

To develop an in-depth understanding of the role that the physicochemical properties of nanomaterials have on overcoming the different biological barriers that can impede nanoparticle accumulation in tissue.

• Cellular Targeting of Bispecific Antibody-Functionalized Poly (ethylene glycol) Capsules: Do Shape and Size Matter? This paper examines PEG particles functionalised for targeting with interesting bi-specific antibodies and made in several sizes and shapes. Notably, it finds that when you control for dosage effects experimentally, there is no difference in association for differently shaped/sized particles (normalised to surface area)

Value • This work has fundamental scientific importance for understanding cell biology in vivo. • Development of the zebrafish as a system to study endocytosis will usher in a new phase of in vivo cell biology; • A universal method for studying nanoparticles in a simple model in vivo system will be of great interest to scientists and will have commercial benefits; optimising targeted nanomaterials to provide fundamental insight into improved formulations in future therapeutics.

Outcome/s • An increased understanding of how a protein or nanoparticle passes from the bloodstream into tissues, and how it is taken up by target cells.

Activities planned for 2020 • Quantitative analysis of nanoparticle trafficking in vivo; Mechanisms of, and imaging, the transport of nanoparticles across the endothelium; Enhancing selective association and uptake into cells in vivo; Investigations into mechanisms of endosomal escape • How does targeting affect biodistribution? What is the optimal targeting density to effect both tissue accumulation and target cell association?

• Study and optimisation of endosomal escape in whole animals.

CBNS Annual Report 2019 19

R E S EAR C H

Collaborating organisations/groups: Professor Cameron Alexander (University of Nottingham), Professor Maria Kavallaris AM (UNSW), Professor Stephen Kent and Dr Georgina Such (UoM), Professor Chris Porter and Dr Angus Johnston (Monash), Professor Steve Mahler (UQ), Dr Brad Walsh (Minomic International)

Mediating protein interactions Leaders: Dr Pu-Chun Ke, Professor Frank Caruso, Professor Pall Thordarson Co-Leaders: Dr Yi (David) Ju, Ms Emily Pilkington, Dr Adam Martin Collaborating organisations/groups: Professor David Haddleton and Professor Greg Challis (University of Warwick), Professor Kenneth Dawson (University College Dublin), Professor Morgan Alexander (University of Nottingham), Professor Raffaelle Mezzenga (ETH Zurich), Professor Feng Ding (Clemson University), Dr Darren Creek (Monash), Dr Paul Wilson (UQ), Professor Sijie Lin (Tongji University), Professor Maria Kavallaris AM, Professor Martina Stenzel and Professor Lars Ittner (UNSW), Professor Stephen Kent and Professor Frances Separovic (UoM), Dr Esteban Gurzov (Université libre de Bruxelles), Professor Killugudi Swaminatha Iyer (University of Western Australia), Professor Fiona Wood (Royal Perth Hospital burns unit)

The project

Activities undertaken in 2019

We aim to develop strategies for controlling protein and peptide structure and aggregation, and to understand which biological signals are important to sustain and promote cell growth in biomaterials. Central to these aims is to better understand the mechanism that underpins protein and peptide self-assembly, folding, and misfolding. The knowledge obtained from this work will be essential for developing more effective strategies against amyloid diseases and protein fouling.

• Determined blood plasma interactions and bio-distribution of non-spherical polymersome/peptide interactions.

Value This work will enable cancer-targeting peptides to be used in the treatment of hard-to-cure children’s brain cancers such as medulloblastoma. The peptide-polymer system could be applied in a more generic manner for drug delivery. This research will shed light on the molecular mechanisms of amyloid diseases and offer nanomedicinal solutions to target ageing diseases that are now global epidemics.

Outcome/s Clinical potential of cancer-cell targeting peptide systems established. The formation of a novel zebrafish model has enabled high throughput studies of type 2 Diabetes and Alzheimer’s, as an inexpensive and high throughput alternative to the current mouse models. Domestic (Florey Institute) and international (China, Belgium, US, Switzerland) collaborations have been established to ensure the success of these efforts. Particle properties and the biological environment influence particle targeting in complex biological media.

• Developed a peptide-RNA delivery vehicle. • Alzheimer’s-like pathogenesis studied with a zebrafish model and their mitigation with gold nanomedicine demonstrated. • Real-time imaging of single amyloid protein aggregation. • Immune response to amyloid proteins in aggregation profiled with a localised surface plasmon resonance platform • Optimised the stealthiness and targeting of a cancer cell targeting peptide-functionalised non-spherical nanoparticle. • Investigated the immune cell interactions with a panel of nanoparticles with different physicochemical properties in complex biological environment.

Activities planned for 2020 • To develop a universal inhibitor against the amyloidosis of Alzheimer’s, Parkinson’s and type 2 Diabetes • To examine the endothelial leakiness induced by nanomaterials of changing morphology • To understand the impact of bacterial functional amyloid in cross seeding amyloid beta and alpha synuclein via the gut-brain axis • To understand protein-metallic phenolic network interaction for antifouling and biosensing • To develop non-spherical polymers with cancer targeting peptides • To develop more efficient peptide-drug conjugate for targeting medulloblastoma with better blood-brain-barrier transport characteristics. • To understand the role of protein corona on particle-immune cell interactions by combining proteomics and bioinformatic analysis.

Peptide aggregation and stabilisation in human pancreatic islets. An ellipsoidal polymersome with tuneable size and shape properties functionalised with medulloblastoma cell targeting peptides.

20 CBNS Annual Report 2019

SENSORS & DIAGNOSTICS New tools for quantifying and manipulating rare entities directly into biological fluids Leaders: Professor Justin Gooding, Professor Benjamin Thierry, Dr Simon Corrie Collaborating organisations/groups: Professor Stephen Mahler and Professor Kris Thurecht (UQ), Professor Pall Thordarson, Professor Maria Kavallaris AM (UNSW), Associate Professor Therese Becker (Ingham Institute for Applied Medical Research), Professor Katharina Gaus (EMBL Australia Node in Single Molecule Science), Professor Wayne Tilley (University of Adelaide), Dr Luke Selth (Flinders University), Dr Clare Whitehead (University of Toronto), Dr Deirdre Zander-Fox (Monash IVF), Dr Tristan Hardy (Repromed – Monash IVF, Medical Device Partnering Program), Professor William Rawlinson and Dr Sacha Stelzer-Braid (NSW Health Pathology).

The project

Outcome/s

The unmet need in the biosensing field is to develop technologies that can detect, quantify and even manipulate rare biomarkers. This is important for early disease diagnosis, infection detection and the monitoring and handling of rare cells. Currently, technologies can detect species down to the nanomolar range whereas the amount of biomarker for the early diagnosis of disease is typically in the picomolar range or lower. In the case of rare cells, the requirements may be to detect and manipulate one in a billion cells. The challenges with such ultrasensitive sensors are having more sensitive transducers, mass transport of the biomarkers to the sensor in a reasonable time frame and achieving good selectivity. This project will seek solutions to these three main challenges that will allow the monitoring of rare biomarkers directly in bodily fluids with minimal sample preparation. The focus will be on rare protein and nucleic acid biomarkers and the manipulation and characterisation of rare mammalian and bacterial cells. The solutions will be integrated into platform technologies that will comprise the next generation of precision sensors. These technologies will be end-user driven to foster the growth of the Australian diagnostics device industry centred around the liquid biopsy concept.

• The detection and quantification of rare biomolecular entities directly in biological fluids, with minimal sample preparation and without calibration or amplification.

Value End-user driven development of liquid biopsy technologies will address crucial health needs and also foster the further growth of the Australian medical diagnostic device industry.

Below: 3D illustration showing a biofilm formed in vivo containing antibiotic-resistant bacteria. Once microbial biofilms are established, they are significantly less susceptible to antibiotics. Furthermore, most BSIs originate from biofilms formed on medical devices or anatomical sites.

• Further detection, characterisation and manipulation of rare mammalian and bacterial cells in whole blood. • Integration of these advanced separation and detection advances within platform technologies towards the development of the next generation of precision sensors.

Activities undertaken in 2019 Development of assays for ultra-sensitive (e.g. atto-molar) detection of rare events following separation, including: blood-tube assays for bacterial cell detection, microfluidic systems and, solid-state electrochemical and/or nanopore based systems.

Activities planned for 2020 • Development of a point-of-care testing device for the detection of pregnancy complications using a field effect transistor biosensor integrated with autonomous plasma extraction technology. • Development of streamlined processing approaches to improve the isolation of rare mammalian cells (e.g. fetal cells) in whole blood and their subsequent analysis. • Development of a single molecule sensing strategy for detecting viral DNA and RNA from different influenza strains and other viruses. • Repurposing of a commercial glycose biosensor for the detection of proteins and other species related to infections.

CBNS Annual Report 2019 21

R E S EAR C H

Co-Leaders: Dr Marnie Winter

IMAGING & VISUALISATION JTCC: Visualising bio-imaging and data in 3D virtual reality Leaders: Associate Professor John McGhee, Professor Tom Davis Co-Leaders: Dr Andrew Lilja, Dr Shereen Kadir, Dr Rowan Hughes, Mr Campbell Strong, Mr Nick Gunn Collaborating organisations/groups: Professor Cameron Alexander (University of Nottingham), Professor Jason Lewis (Memorial Sloan Kettering Cancer Center), Associate Professor Steven Faux (St Vincent’s Clinic), Professor Maria Kavallaris AM (UNSW), Professor Rob Parton and Professor Kris Thurecht (UQ), Dr Angus Johnston (Monash)

The project

Outcome/s

This project will explore how design-led 3D virtual reality (VR) immersive techniques can be used to visualise the dynamics process within a cancer cell. The research also intends to conduct user testing of the VR content with students and scientific communities. The goal of this research is to establish whether VR immersive data interaction provides benefits as an educational tool. We also intend to investigate whether 3D VR visualisations of laboratory data can facilitate and enhance the scientific discovery process. As part of this evaluation, we will be developing a VR platform to ‘share’ content. The goal is to connect VR 3D cellular data between multiple users and sites across the CBNS nodes simultaneously on a collaborative VR platform, to be termed the ‘VR Cell Arena System’.

• Nanoscape – Peer review high impact Non-traditional outputs (NTRO) – VR Film and interactive environment

Sub-projects

• Education Vis/Representation nanostructures development and evaluation

• 3D Vox-Cell Volumetric Data Visualisation – UNSW, UQ, Monash • Nanoscapes: Interactive Real-Time Cellular Landscape Exploration – UNSW, UQ, Monash • MultiUser VR of High-Resolution Cellular Dynamics Data – UNSW, CCIA • 3D VR in vivo Nanoparticle Dynamics Data – UNSW, UQ

Value The development of effective VR tools and immersive narratives for communication in education, about nanoparticle drug delivery, will allow a new type of aesthetic for the public to view and experience nanoparticle science in VR. This will lead to a better understanding of alternative modes to visualise nanostructures in both education and science discovery settings.

A concept illustration showing the chaotic vista on the cell surface in Nanoscapes. Image credit: Andrew Lilja, John McGhee.

22 CBNS Annual Report 2019

• VR interactive platform for importing and realising visualisation of nanoparticles in vivo • A knowledge base on interactive VR uses in education, science discovery and public engagement and nanoparticles in vivo • 3D interactive Vox-Cell Volumetric Data Visualisation viewer

Activities undertaken in 2019 • Nanoscape 3D real-time experience, Realtime engine film and 3D interactive VR experience

• Interaction Evaluation – Mouse VR and Multiuser system • Development of VR work with EMBL

Activities planned for 2020 • Completion of Nanoscape 3D real-time experience, Realtime engine film and 3D interactive VR experience • Evaluation results of user test – VR interactive platform for importing and realising visualisation of nanoparticles in vivo. This Project is further supported by our Strategic Fund. More information is documented in Strategic Projects on Page 31.

Protein look development for Nanoscapes: various style options shown for EGFR to maintain real-time performance. Image credit: Andrew Lilja. John McGhee.

R E S EAR C H The main features of Nanoscapes shown at relative scales to the viewer. Image credit: Shereen Kadir, John McGhee.

Towards more authentic scales and densities in cellular visualisations. An early prototype of the interactive cellular landscape of Nanoscapes. Image credit: Nick Gunn, John McGhee.

CBNS Annual Report 2019 23

Overarching Projects Social dimensions of bio-nano interactions Leader: Associate Professor Matthew Kearnes Co-Leader: Dr Declan Kuch Collaborating organisations/groups: Professor Stephen Kent (UoM), Professor Justin Gooding, Associate Professor Orazio Vittorio and Professor Maria Kavallaris AM (UNSW), Professor Kris Thurecht and Professor Rob Parton (UQ), Dr Angus Johnston (Monash)

The project

Activities undertaken in 2019

Bio-nano technologies, together with advances in precision and personalised medicine, are likely to profoundly change health care practices. By exploring the social dimensions of research across this area of work, in collaboration with key CBNS research initiatives, this program will provide insights into the societal dimensions of predictive bio-nanotechnologies. This project seeks to address key questions related to the intersection between big data, healthcare, personalised and precision medicines, and regulation. The proposed program of work will entail the use of the following social science methodologies, and will be facilitated by a range of cross-node collaborations.

Events • Coordinated the research symposium: Biomedical Futures: Values, Responsibility, Critical Engagement in Nanotechnology and Electromaterials, a collaboration between the CBNS & the ARC Centre of Excellence for Electromaterials Science (ACES).

These methods include: • Social media monitoring and analysis tools (using tools such as NodeXL) will enable issue mapping of the institutional and discursive shaping of research agendas in precision, and personalised medicine; • Ethnographic observation: close analysis of science-inpractice will serve to document the imagined social worlds that underpin developments in bio-nanotechnology, focusing specially on CBNS research projects; • Interdisciplinary exchange workshops will bring CBNS researchers into conversations with researchers working in the social sciences, humanities and law to explore the broader social dimensions of their work; Targeted public engagement initiatives will also form part of the work plan of the program, enabling Centre researchers to address the societal dimensions of their research in appropriately designed and facilitated public forums.

Research Research for the social dimensions signature project was enabled by a series of research collaborations across CBNS. These include ongoing research exploring the Enhanced Permeability and Retention (EPR) effect, the visualisation of nanoscale interactions, the use of nanoparticles in the development of vaccines, and the social and ethical dimensions of organoids and lab-on-a-chip systems.

Value Characterising the sociological dimensions of research in bio-nano science and technology has broad importance for the general public and societal understanding and acceptance of bio-nano science applications in the real world.

24 CBNS Annual Report 2019

This symposium included presentations from CBNS researchers CI Associate Professor John McGhee, Dr Declan Kuch, and Associate Professor Matthew Kearnes (UNSW) alongside prominent HASS researchers exploring the social and ethical dimensions of nanotechnology and novel technologies Professor Susan Dodds (La Trobe University) and Dr Eliza Goddard (La Trobe University). • Coordinated the Social Aspects & Regulation stream at the 10th International Nanomedicine Conference June, 2019, including keynote presentation from Professor Rachel Ankeny (University of Adelaide) on Animal Models in Biomedicine: Past, Present, and Future Directions. Policy Engagement • Invited contribution on ‘Agricultural Nanotechnologies, Public Engagement and Regulation’ for the Future of Agricultural Technologies Project Horizon Scanning Project, Australian Council of Learned Academies (ACOLA). Publications • Publications in Science, Technology & Human Values, Journal of Environmental Policy & Planning, Policy Studies and a forthcoming research monograph manuscript on based on research conducted in collaboration with CBNS.

Activities planned for 2020 A series of research events will be coordinated throughout 2020, including a CBNS workshop on ‘responsible innovation’ and new methodologies for responding to questions pertaining to the social aspects of bio-nano research.

Predicting bio-nano interactions Leaders: Professor Stephen Kent, Professor Edmund Crampin Co-Leaders: Dr Adam Wheatley, Dr Matt Faria Collaborating organisations/groups: Professor Molly Stevens (Imperial College London), Professor Robert Parton and Professor Kris Thurecht (UQ), Dr Angus Johnston and Dr Simon Corrie (Monash), Professor Frank Caruso (UoM)

Understanding how materials and cells interact will be key to the future development of improved nanomedicines and vaccines. We aim to understand the rules by which immune cells interact with a range of nanoengineered particle systems with tailored physical properties. The combined effects of size, charge, surface chemistry and other physio-chemical characteristics will be studied for their effect on particle interactions with a wide range of immune cells.

Value Understanding how materials and cells interact will be key to future development of improved nanomedicines and vaccines. • The experimental and theoretical techniques we develop will provide a roadmap for biological analysis of newly developed nanomaterials for the global research community.

definitions for the latter. It also represents one of the most “complete” datasets on biological characterisation of a particle, including both cell-level response and proteomic response in whole human blood. • Progress in standardisation: positive response from the community that Nature Nanotechnology organised, multiple editorials, analysis, and responses from more than 20 international research groups and further promotion in the Journal of Controlled Release of the Minimum information reporting in bio–nano experimental literature.

Activities planned for 2020 • Development and publication of kinetics-based particle approaches applied to complex in vitro systems under continuous flow.

• Our fundamental understanding of the relative importance of nanoparticle physicochemical properties will be advanced, leading to a more rational design of these materials. • By increasing our ability to predict bio-nano interactions prior to clinical evaluation, we will vastly accelerate our ability to evaluate and design nanoparticles for diagnostic and therapeutic purposes.

Outcome/s This work will increase our understanding of the rules by which immune cells interact with a range of nano-engineered particle systems with tailored physical properties. Our research examines the influence, and trends of different physicochemical characteristics of nanoparticles, on their interactions with immune cells in vitro and in vivo. This research will demonstrate the potential to standardise the way in which nanoparticle-cell interactions are studied, such that we can generate predictive algorithms for these interactions.

Summary of cell-particle kinetic modeling. Characterisation information about cells and particles along with details of experimental protocol are used as parameters in a given mathematical model, which accounts for both dosimetry and the kinetics of cell-particle association. Ultimately, the fit kinetic parameters, which are the output of this modeling, can enable unbiased, quantitative comparison between in vitro experiments that vary in cell line, experimental protocol, and/or particle type.

Activities undertaken in 2019 • Revisiting cell-particle association in vitro: A quantitative method to compare particle performance – this paper is a comprehensive approach to getting quantitative data on cell-particle interactions in vitro. Several potential kinetic models of cell-particle interaction are compared. The techniques developed within allows for boiling down “cell-particle interaction” into a single number, which for the first time can quantitatively represent the targeting or stealth performance of a particle. • This paper has a companion webpage at http://bionano.xyz/ estimator, which allows researchers without an experimental background to apply the sophisticated kinetic modelling techniques to their data without a mathematical background • Link between low-fouling and stealth: a whole blood biomolecular corona and cellular association analysis on nanoengineered particles – this paper differentiates and goes into some detail about the difference between “stealth” (non-interaction with the immune system) “low-fouling” (interaction with proteins), and explores multiple potential

Atomic force microscopy (AFM) and transmission electron microscopy (TEM) images of synthesised particles. (a–c) AFM images and corresponding cross-sectional AFM profiles of PMASH capsules with diameters of 1032 nm (a), 480 nm (b), and 214 nm (c). (d–j) TEM images of PMASH core–shells of 633 nm (d), 282 nm (e), 150 nm (f), 95 nm (g) and capsules with diameters of 1032 nm (h), 480 nm (i), and 214 nm (j). Scale bars are 500 nm in (d, h, i, j) and 100 nm in (e, f, g).

CBNS Annual Report 2019 25

R E S EAR C H

The project

Development of complex cellular systems for the evaluation and characteristics of bio-nano interactions Leaders: Professor Maria Kavallaris AM, Professor Benjamin Thierry Co-Leaders: Dr Frieda Mansfeld, Dr Michelle Maritz Collaborating organisations/groups: Professor Jason Lewis (Memorial Sloan Kettering Cancer Center), Professor Cameron Alexander (University of Nottingham), Professor Richard Lock (Children’s Cancer Institute)

The project

Activities planned for 2020

A major challenge in the development and implementation of effective nanomedicine, is the lack of preclinical models that recapitulate the complexity of the complex cellular systems and microenvironments. Towards accelerating the development of nanotechnology strategies that target specific organ and cellular systems, we are developing the next generation of in vitro models designed to replicate physiological and biological systems relevant to the characterisation and evaluation of bio-nano interactions. Ultimately, these advanced models will guide the development of nano-based diagnostic and therapeutic strategies better tailored to specific diseases.

• Further develop microfluidic and 3D tumour models to extend understanding of nanoparticle interactions with and transport across biological barriers and through tissues.

Value The development and characterisation of bioengineered microfluidic models will enable accurate prediction of healthy and tumour tissue responses to cancer therapeutics as well as an assessment of novel nano-based nanoparticle interactions for broader applications.

• Complement biological models with mathematical modelling of bio-nano interactions. • Head and neck cancer patient derived organoids are being developed within a microfluidic air-liquid interface that main the tumour microenvironment. • Bioengineered tumour models are being used to developed radiogenemic models predictive of radioresistance • Intestine-on-chip models are being developed towards supporting the in vitro growth of cryptosporidium and norovirus. • Placenta-on-chip models are being developed to investigate the interaction between nanoparticles and placental tissues. Bioprinted tumouroids

Outcome/s Bioengineered microfluidic and 3D bio-printed models will enable the prediction of more physiologically relevant responses of healthy and disease tissue responses and serve as a more relevant pre-clinical model for nano-based therapies for cancer and other diseases.

Activities undertaken in 2019 • Professor Jason Lewis visited several nodes in January 2019 and in January 2020 to strengthen collaborations and provided mentorship to junior researchers

An intestine-on-chip to better study particles’ cellular uptake and interactions with mucus. Biomaterials Science 2019

• Developed and characterised bioengineered microfluidic vascularised tumour and organ-on-chip models for assessment of nano-based therapeutics • Advanced 3D bioprinting technology to produce and characterise tumouroids, complex in vitro models incorporating patient derived tumour cells • The effect of the fluidic condition on the phenotype and function of intestine-on-chip has been systematically investigated • Intestine-on-chip models have been used to investigate the binding and transcytosis of nanoparticles

Brightfield images of 96-well plate with bioprinted patient derived tumouroids. Right: Enlargements of two tumouroids shows consistency in size and morphology. (Image: Lakmali Attapattu)

26 CBNS Annual Report 2019

Improved molecular imaging agents Leaders: Professor Andrew Whittaker, Professor Tom Davis Co-Leaders: Dr Jeroen Goos, Dr Ruirui Qiao, Dr Cheng Zhang, Dr Changkui Fu, Dr Simon Puttick

The project

Activities planned for 2020

The project team aims to improve the resolution and accuracy of molecular imaging at different physiological sites and to exploit nanotechnology for specific diagnosis and theranostic applications.

In 2020, we plan to develop advanced molecular imaging agents that will include development of multimodal imaging agents, using molecular dynamics simulations to understanding interaction of imaging agents with biological systems such as proteins, cells and in vivo system, and development of long-circulating nanoparticular imaging agents for more efficient and targeted imaging.

Value There is currently a re-evaluation of several aspects of nanomedicine, in particular delivery efficiency. Imaging agents will play a crucial role in advancing the field by enabling quantitation and higher-level information in the case of multi-modal or responsive agents.

Outcome/s Imaging is a central technology in the field of nanomedicine. There are a plethora of current approaches; the primary outcome of this overarching project will be a clear direction on the design and choice of imaging agent.

Superposition of PET/CT images of a mouse with a brain tumour xenograft, after injection with a 64Cu labelled antibody.

Activities undertaken in 2019

PET/CT showing the distribution of a functional star polymer (S. Puttick/M.R. Whittaker), showing uptake in the bone marrow.

• Study biological interactions: 1. Blood-NP interactions 2. Biological fate 3. Protein corona • Develop an understanding of relationship between nanoparticle design and these interactions. From this, design responsive agents. • Development of advanced imaging agent with improved sensitivity We have developed several advanced MRI contrast agents with improved sensitivity, including particularly some biocompatible metal-free polymeric 19F contrast agents. The Whittaker group has developed an innovative class of water-soluble fluorinated homopolymer (PFSAM) with high fluorine content that is suitable as a highly-sensitive 19 F tracer for bioconjugation and in vivo tracking (Angew. Chem. Int. Ed.2020, 59, 2–9). PFSAM is made of a carefullydesigned sulfoxide-containing fluorinated monomer (FSAM) and demonstrates interesting solution properties. Compared with previously-reported polymeric 19F CAs, PFSAM can enable more versatile biological applications such as in facile conjugation with therapeutics in various forms (e.g., peptides, proteins, or nanoparticles) to develop next-generation of self-trackable polymer therapeutic conjugates. Moreover, PFSAM is low-fouling interacting weakly with biological system. This is particularly important for the development of long-circulating diagnostics and therapeutics with improved therapeutic outcomes.

Snapshots of MD simulation of bovine serum albumin interacting with oligomeric polymer acrylic acid taken at the end of 20 ns of MD simulation.

Modulating interaction of imaging nanoparticles with biological system using a highly hydrophilic lowfouling sulfoxide polymer as coating materials for nanoparticles. This will reduce unwanted nonspecific interaction with biological system and improve the accumulation of the imaging nanoparticles to the region of interest to further enhance imaging sensitivity and specificity for more precision detection of diseases.

CBNS Annual Report 2019 27

R E S EAR C H

Collaborating organisations/groups: Dr Ivan Greguric (ANSTO), Professor Jason Lewis (Memorial Sloan Kettering Cancer Center), Professor Thomas Nann (MacDiarmid Institute), Professor Craig Hawker (University of California), Dr Sophie Laurent (Université de Mons), Professor Debra Bernhardt, Professor Maree Smith, Professor Trent Woodruff (UQ), Professor Stephen Rose (CSIRO), Professor Guangjun Nie (National Center for Nanoscience and Technology of China), Professor Afang Zhang (Shanghai University), Professor Zushun Xu and Professor Ling Li (Hubei University), Professor Fabienne Dumoulin (Gebze Technical University), Professor Petr Kral (University of Illinois at Chicago), Professor Simon Swift (University of Auckland), Professor Kishore Bhakoo (A*STAR)

Cross-Centre Activity Bio-responsive nanocarriers for the eradication of bacterial and fungal biofilms CBNS Chief Investigators involved: Professor Clive Prestidge, Professor Ben Boyd and Professor Tom Davis CBNS Researchers: Dr Mikey Whittaker, Dr John Quinn, Dr Paulina Ramírez-García, Dr Andrew Clulow and Dr Nicky Thomas CBNS PhD students: Ms Chelsea Thorn and Ms Yassamin Albayaty The UniSA-Monash cross-node research program aims to explore bacterial and fungal infection responsive nanocarriers for delivery of anti-biofilm agents directly to the site of action, increasing bioavailability and efficacy has continued to gain momentum and yielded positive outcomes.

Antifungal delivery via pH-responsive polymeric micelles We have prepared and characterised pH-responsive micelles composed of the Monash team synthesised block copolymer poly[(N,N-diethylamino)ethyl methacrylate-block-polyethylene glycol methacrylate] (P[DEAEMA-b-PEGMA]) and demonstrated triggered release of the antifungal drug itraconazole within the low pH environment of Candida albican biofilms. Enhanced biofilm uptake, targeted release and improved delivery of itraconazole has resulted in a significantly increased death of fungal cells and biofilm eradication in comparison with non-encapsulated drug. We have successfully quantified the pH-controlled release and determined the mechanism of action. Furthermore, the anti-biofilm efficacy of pH sensitive micelles has been demonstrated on different fungal strains.

KEY GOALS FOR 2020 1 Determine the biological response and effects of the pH-responsive micelles in various fungal biofilms and compare with other polymeric micelle systems. 2

Explore the performance of the pH-responsive micelles for delivery and efficacy improvement of other antimicrobials.

Liquid crystalline lipid nanocarriers for the triggered release of antimicrobial agents and anti-biofilm activity Liquid crystalline lipid nanocarriers based on glyceryl monooleate (GMO) have been shown to effectively encapsulate a combination of large enzyme molecules (e.g. glycoside hydrolases that disrupt the extracellular matrix of biofilms) and small antibiotic molecules of varying hydrophilicities and to facilitate their improved penetration and triggered release within bacterial biofilms. The controlled release has been shown to be partially driven by the digestive action of lipase enzymes produced by bacteria within biofilms. Under biorelevant conditions, the liquid crystalline lipid nanocarriers facilitate significant enhancement in the stability of encapsulated biological antimicrobials, e.g. novel glycoside hydrolases. Additionally, the safety and efficacy of GMO cubosomes have been determined in various in vitro biological models and comparisons made with non-digestible liquid crystalline lipid nanocarriers and liposomes. Initial results are strongly positive and have encouraged ongoing investigations of the mechanisms of actions and application focused in vitro and in vivo studies.

KEY GOALS FOR 2020 1 Advance anti-biofilm efficacy studies for a range of encapsulated antimicrobials (small and large molecules). 2

Yassamin Albayaty, Nicky Thomas, Paulina Ramírez-García, Thomas Davis, John Quinn, Michael Whittaker and Clive Prestidge, “pHResponsive copolymer micelles to enhance itraconazole efficacy against Candida albicans biofilms”, Journal of Materials Chemistry B, 8, 1672-1681, 2020

28 CBNS Annual Report 2019

Gain further insight into the mechanisms of action.

Chelsea Thorn, Andrew Clulow, Ben Boyd, Clive Prestidge, Nicky Thomas, “Bacterial lipase triggers the release of antibiotics from digestible liquid crystal nanoparticles”, Journal of Controlled Release, 319, 168-182, 2020

Strategic Projects In addition to the core research undertaken in the four Overarching Projects and five Signature Projects and the Centre-wide research which is detailed in the previous pages, the CBNS provides funding for activities that meet the strategic objective of the CBNS to leave a legacy of research excellence and innovation, together with a highly skilled and professional workforce, ready to lead bio-nano science research and technology into the future.

When assessing proposals for strategic funding, the CBNS Executive Committee consider whether the projects: • Involve collaboration between at least two CIs, or members of their research groups;

• Involve collaboration between at least two nodes; • Introduce skills, expertise or activities not presently available within the Centre; • Have a duration no longer than two years (although after two years, successful projects can apply for additional funding to continue the project);

• Address at least one non-research KPI (such as media, public or industry engagement, visitors, training etc.); and • Not exceed an annual cost of $100,000. In addition to small amounts of funding supporting student attendance at workshops, three major projects were funded in 2019. They are detailed in the following section.

CBNS Annual Report 2019 29

R E S EAR C H

Strategic Projects are assessed by all Centre CIs and are supported by a dedicated fund. The strategic funding is managed centrally and all collaborating organisations contribute part of their ARC funding each year.

Open Data Fit Leader: Professor Pall Thordarson Co-Leaders: Professor Tom Davis, Professor Ben Boyd, Dr Simon Corrie, Professor Edmund Crampin, Professor Maria Kavallaris AM The Open Data Fit project1 is creating a portfolio of websites that bring together tools and information of relevance to the CBNS as well as the broader international research community. The project focuses on what we call small-scale data, i.e. the small data sets that are the bread-and-butter of research in bio-nano research, ranging from kinetic measurements (kinetics) and small-angle scattering curves (nanoparticle characterisation) to cell viability measurements (IC50 for drug treatments). The goal of the Open Data Fit is, therefore, to shine a light on smallscale dark data and transform the way scientists share small-scale datasets. To this end, we designed the Open Data Fit

project to assist researchers with their everyday work while at the same time capturing the raw data for productive sharing. The opendatafit (http:// opendatafit.org) web portal (tools) we are building will achieve this by allowing the end-user scientist to upload their data and then perform data fit (analysis) on these datasets, while at the same time capturing the raw data, the analysis method and the results simultaneously in an open-access database. With CBNS support, we built and successfully launched our pilot website supramolecular.org in 2015. This website provides tools for end-users to determine binding constants from NMR, UV-Vis or fluorescence titration data. Since it was launched, it has had over

1. Hibbert D. B., & Thordarson P., The death of the Job plot, transparency, open science and online tools, uncertainty estimation methods and other developments in supramolecular chemistry data analysis Chemical Communications 2016, 12792-12805.

30 CBNS Annual Report 2019

40,000 visits with the average session lasting 3:30 minutes, showing a high level of engagement from end-users. Moreover, the website has been cited 120 times in the literature (Scopus). Significantly, Sir Fraser Stoddart, Nobel Prize Winner in Chemistry in 2016, agreed to become the Patron of this project. In 2019 we saw a step-change in how the project is influencing the conversations about data management both nationally and internationally. In March 2019, the program lead was invited to an NSF-sponsored workshop in Orlando, Florida on FAIR Chemical Data Publishing Guideline Workshop. The exclusive audience included representatives from all the major publishing companies (Springer-Nature, Science, Wiley, RSC, ACS) and as well IUPAC, NSF, NIST and the NIH. The Opendatafit project was one of the five exemplary Chemical Data Reuse Case Studies presented at this meeting. Later in the year Opendatafit effectively formed one of the major platforms within a national consortium named the Australian Characterisation Commons at Scale (ACCS). The ACCS was then successful in obtaining a $1.9-Milliondollar platform grant from the Australian Research Data Commons (ARDC). With co-investment from University partners, this is a $5.3M project running over three years with the Opendatafit component, the â&#x20AC;&#x153;National Tools for Scattering and Beyondâ&#x20AC;? receiving about $360K of this funding package to grow and expand the Opendatafit platform the next three years.

JTCC: visualising bio-imaging and data in 3D virtual reality (VR) Leaders: Associate Professor John McGhee, Professor Tom Davis Co-Leaders: Dr Andrew Lilja, Dr John Bailey, Mr Campbell Strong, Dr Rowan Hughes, Dr Shereen Kadir

Key projects within the JTCC Signature Project made significant progress in 2019 with the appointment of lead Unity developer Nick Gunn, allowing an increased focus on real-time data visualisation and user engagement. Development of the core projects Nanoscapes and Vox-Cells remained high priorities, with both gaining international conference attention. Other ongoing projects, including the VR visualisation of in vivo nanoparticles and multi-user data analysis platforms continued as core areas of research with an increased emphasis on user testing and evaluation as science discovery and education tools.

Sub-projects: 1. 3D Vox-Cell (UNSW, UQ, Monash) This project tackles the standard approach to volume surface extraction and rendering, to provide both investigators and the lay viewer with a new look in on volumetric datasets. We developed a novel approach for efficient construction and lighting of mesh surfaces extracted from 3D volumetric data sets. Several refinements were made to allow for real-time visualisation of complex data sets, making the system ideal for data inspection in VR. The innovative approach and lighting algorithms were provisionally patented and caught the attention of industry partners. Formal user evaluation of the system will be completed in 2020. 2. Nanoscapes (UNSW, UQ, Monash) – Key project for 2020 This ambitious project aims to bring more authentic scales and densities of biological entities to real-time

cinematic visualisations of cellular landscapes. Built using the PC gaming platform Unity, Nanoscapes employs a data-first approach to populate a computer-generated cell surface with key molecules and processes in order to observe the action of nanoparticles in tumour environments. The application serves as both an interactive educational tool and a provoking artefact promoting deeper speculation about nanoworlds. 3. Multi-user VR visualisation of highresolution cellular dynamics data (UNSW, CCIA) This project visualises multiple, dynamic cellular imaging data sets (captured by light sheet lattice microscopy) in an immersive and collaborative virtual environment. This project continues to progress steadily with a core focus on the of development of networked multiuser VR that facilitates longdistance collaboration and cooperation. This work was presented in 2019 at the flagship conference for VR in industry applications (VRCAI), highlighting features including remote voice chat, personalised avatars and data manipulation functionality. 4. Design-led 3D VR of in vivo nanoparticle dynamics data (UNSW, UQ) This project aims to visualise CT and PET data in VR to identify the precise number and location of nanoparticles following in vivo administration. The project has made good progress towards formal evaluations involving students and staff at UQ. User testing, scheduled for early 2020, will probe the effectiveness and usability of the application as a tool for data analysis and education.

Publications: 1. BJ Bailey, A Lilja, C Strong, K Moline, M Kavallaris, RT Hughes, J McGhee, Multi-User Immersive Virtual Reality Prototype for Collaborative Visualization of Microscopy Image Data, VRCAI ’19, Nov 2019, Brisbane, Australia. 2. RT Hughes, C Strong, J McGhee, Vox-Cells: Voxel-based visualization of volume data for enhanced understanding and exploration in Virtual Reality (VR), ACM SIGGRAPH 2019 Posters, July 28 – Aug 1, Los Angles. 3. AR Lilja, S Kadir, RT Hughes, NJ Gunn, C Strong, BJ Bailey, RG Parton, J McGhee, Nanoscapes: Authentic Scales and Densities in Real-Time 3D Cinematic Visualizations of Cellular Landscapes, SIGGRAPH Asia 2019 Posters, Nov 2019, Brisbane.

KEY GOALS FOR 2020 1 User testing and evaluation of the Vox-Cell volumetric data visualisation tool 2 Finalise the Nanoscapes interactive application and undertake formal evaluation of its educational and speculative potential 3 Formal user testing to evaluate the effectiveness of immersive VR as a platform to visualise and interact with nanoscience laboratory data compared to traditional screen-based methods 4 Refine and test embodied interfaces that allow remote multi-user immersive 3D VR data interaction

PET-CT imaging data sets viewed within the Vox-Cell visualisation system. Image credit: Rowan Hughes, John McGhee, Kris Thurecht, Zach Houston. Nanoparticle and protein look development for real-time performance in Nanoscapes. Image credit: Andrew Lilja, John McGhee.

CBNS Annual Report 2019 31

R E S EAR C H

2019 progress

The neuro-nano interface – using nanotechnology to provide a window into pain Leaders: Dr Nik Veldhuis, Professor Kris Thurecht, Associate Professor John McGhee Co-Leaders: Dr Daniel Poole, Dr Paulina Ramírez García, Dr Mikey Whittaker and Associate Professor Matthew Kearnes The two-year project aims to bridge the gap between neuroscience and nanotechnology and provide new insights and new outreach/education opportunities through visualisation of pain states and associated discussion about the potential for nanotechnology to change drug targeting and drug properties for pain. Challenges for nanotechnology beyond cancer Encapsulation into nanoparticles (NPs) can improve the therapeutic effectiveness of drugs by enhancing stability, tolerability, delivery and retention in diseased tissues. There is particular interest in using NPs to deliver anti-cancer drugs, perpetuated by the prospect of engineering NPs to target tumour cells via the so-called EPR effect – exploiting the leaky vasculature and poor lymphatic drainage of solid tumours to promote NP accumulation and uptake. In addition to the development of cancer therapies, there are many potential applications for drug delivery in other conditions and where leaky vessels and edema may occur, or where cellular environments change (e.g. shifts in pH or reactive oxygen species). For example, inflammation and infection lead to edema or swelling, and acidification of extracellular microenvironments and this can be exploited by uptake pH-responsive nanoparticles for exclusive drug release in an affected area to increase drug efficacy and avoid side-effects. As a part of the Signature Project A material scientist’s guide to the cell, our collaborative team in the CBNS proposed that using nanoparticles for intracellular drug delivery would be an effective strategy for the delivery of poorly distributed analgesic or antiinflammatory drugs, and may improve analgesic outcomes. However, our understanding of nanotechnology and in particular, bio-nano interactions with central or peripheral nervous systems is very limited. We have a dedicated program for delivery of drugs to endosomal compartments within neurons of the spinal cord, and seek to extend these studies to other

32 CBNS Annual Report 2019

neurons and also increase awareness of opportunities for nanotechnology in modulating neuronal processes. Harnessing nanotechnology to provide a window into pain transmission and analgesia Chronic pain is a global problem with few effective treatment options, and serious societal and pharmacological challenges due to over-subscription of opioids, leading to widespread addiction and significant increases in opioidrelated deaths. The CBNS has an opportunity to capitalise on the momentum of our current neuro-focussed nanotechnology and the current highly publicised ‘opioid epidemic’, through imaging and visualisation, to provide more significant insights into pain processes and addressing key questions in neurobiology: – How, where and when does pain occur? – How can we harness nanotechnology to improve treatment options? – Can nano-based diagnostics or biosensors be developed to allow us to “visualise pain”

Sub-projects: 1. Visualising nanoparticle effects in neurons (Monash, UQ) Background – There remains a need for visualisation and models to allow scientists, students and the public to explore/conceptualise the use of nanoparticles for “fine-tuning”

signalling processes, rather than releasing cytotoxic payloads for treating cancer. We will generate a series of visualisations to show how nanoparticles improve delivery. Altering intracellular drug distribution – We have recently generated a new suite of fluorescently labelled nanoparticles (pH-responsive micelles and crosslinked stars) to generate 3D images of fluorescent nanoparticle uptake into neurons of the spinal cord. We are currently using confocal microscopy on spinal cord slices and if possible, we will also apply a powerful new lightsheet microscopy technique that can image through deep tissue (whole spine). This will provide new visual insights to demonstrate that nanomaterials and drugs can be directed to pain-inducing targets in spinal neurons. Targets include the Neurokinin 1 Receptor, which moves to intracellular endosomes to drive neuropathic pain (green stain, figure 1). Biosensor and Nanomaterial uptake in painful conditions – The development of nanomaterials that ‘light up in locations where pain occurs, will allow us to visualise pain over time. In collaboration with ProfessorKris Thurecht, we will track fluorescent- or radio-labelled hyperbranched nanomaterials and intend to explore alternative labelling approaches (radiolabelling or infrared dye) and distinct stimulus-responsive systems, to image distribution and signals in different painful scenarios: In the periphery (e.g. arthritic knee joint) – pH or ROS-responsive polymers label

Fig 1. 3D imaging of Neurokinin 1 Receptor in spinal neurons.

In the spinal cord – increase presence and activity of microglia in the spine as pain develops. There are multiple benefits for generating these data: • Demonstrate nanomaterials as a measure (biosensing) the onset on pain over time, OR assess ability for analgesics to reverse this signal • Assessing if biosensors can give a score that can be overlaid with behavioural pain scores for a more comprehensive assessment of pain preclinical models 2. Visualising pain transmission (Monash, UNSW) To demonstrate how pain signalling occurs in neurons and how nanoparticle may aid analgesia, animated videos will be generated utilising the technology develop by Associate Professor John McGhee, Professor Rob Parton and Dr Angus Johnston for the JTCC project. These will be visually distinct from

previous 3DVAL projects, for visualising neuronal excitability (aka pain) and drug release/distribution. Synthetic neurons will be used in the first instance and new electron microscopy images of cultured neurons, in collaboration with Rob Parton, is also willing to image cultured neurons by electron microscopy, should we need to use real neuronal data to drive the visualisation.

Progress and key goals for 2020: • pH-responsive nanoparticles (disassembling micelles or stable stars) have been synthesised with a Cy5-label for tracking biodistribution over time. • Confocal imaging in spinal neurons with these materials has been initiated and conditions for imaging have been confirmed utilising immunostaining of the NK1 Receptor. • Establishing inflammatory models to demonstrate retention/accumulation of nanomaterials in tissue regions where significant edema has occurred, has been determined and ethics approval is close to being finalised.

• Generation of 3D neuron with the 3D VAL group will being in May, 2020. • Discussions will follow with Associate Professor Matthew Kearnes about developing a narrative for the current concerns when using opioids and the potential uptake of nanotechnology as a new strategy for understanding and combatting pain.

Outreach: N. Veldhuis, “Signalling from the inside out: A new understanding of receptor signalling in neurons”, Keynote presentation at Victorian Teachers Symposium, La Trobe University, Feb 2020

Publications: Paulina D. Ramírez-García, Jeffri S. Retamal, Priyank Shenoy, Wendy Imlach and Matthew Sykes et al. ‘A pH-responsive nanoparticle targets the neurokinin 1 receptor in endosomes to prevent chronic pain’, Nature Nanotechnology, (2019) 14(12):115059, doi:10.1038/s41565-019-0568-x

CBNS Annual Report 2019 33

R E S EAR C H

with infrared dye to indicate the location of inflammatory, acidified tissue or the presence of immune cells.

34 CBNS Annual Report 2019

E N GAG E M E NT

Engagement CBNS Annual Report 2019 35

Collaborations and Partnerships CBNS researchers have a long history of collaboration with other universities, research centres, industry and medical organisations. These collaborations take a wide variety of forms – from formal relationships such as Linkage Projects or contract research, to informal and ad hoc collaborations.

Intellectual Property and Commercialisation Policy The CBNS IP and Commercialisation Committee (IPCC) provides advice for Intellectual Property (IP) management to CBNS CIs. The IPCC comprises the Centre Executive (Director, Deputy Director and Manager), plus the Business Development Manager from the Administering Organisation (Monash). In discussing IP from collaborating organisations, the IPCC co-opts the Business Development Manager (or equivalent) from that organisation.

Intellectual Property CBNS does not own project intellectual property (IP) resulting from CBNS activities. Project IP is owned by the (administering, collaborating or partner) organisation making the major inventive contribution to the IP. Whilst a single organisation assumes control of project IP, in the cases of cross institutional participation in project IP generation, beneficial interest in project IP, is determined by relative contributions to IP generation based on cash and in-kind contributions to that project (cash is weighted by a factor of 2).

36 CBNS Annual Report 2019

CBNS maintains an Intellectual Property Register, which includes up to date details of CBNS IP and each organisation’s background IP. It consists of: • the owners of each item of background and IP and the proportion of ownership • any and all third party interests in background and IP • any patents or patent applications relating to

Commercialisation The organisation owning the project IP supports protection and commercialisation through its IP commercialisation group and meets the cost of protection.

CBNS commercial partners provide real world advice to CIs but also provide a potential path-to-market and a defined route to impact. As such, industry partners have the right of first refusal to a license to exploit project IP on terms to be agreed. In relation to the commercialisation of project IP, all parties negotiate in good faith to agree on a Commercialisation Lead and the terms of any Commercialisation. The Commercialisation Lead has the right to sublicense project IP in the interests of all parties and CBNS, without restricting the use of the project IP for research, teaching and scholastic endeavours.

Node

Type

Date

Inventors/Researchers involved

Monash

Provisional

7 Jan 2019

Chris Porter, Natalie Trevaskis, Tim Quach, Sifei Han, Luojuan Hu & others

PCT

7 Jan 2019

Chris Porter, Natalie Trevaskis, Tim Quach, Sifei Han, Luojuan Hu

Provisional 28 Feb 2019

Chris Porter, Natalie Trevaskis, Dan Zheng, Nathania Leong, Garima Sharma, Mitchell McInerney, Tim Quach, Sifei Han & others

Provisional 28 Feb 2019

Chris Porter, Natalie Trevaskis, Dan Zheng, Nathania Leong, Garima Sharma, Mitchell McInerney, Tim Quach, Sifei Han & others

Provisional 28 Feb 2019

Chris Porter, Natalie Trevaskis, Dan Zheng, Nathania Leong, Garima Sharma, Mitchell McInerney, Tim Quach, Sifei Han & others

Thomas Davis, Jonathan Baell, Jeroen Goos, Jason Lewis

Provisional

17 May 2019

Provisional 27 Jun 2019

Chris Porter, Natalie Trevaskis, Tim Quach, Sifei Han, Dan Zheng, Nathania Leong, Garima Sharma, Mitchell McInerney

Provisional 19 Sep 2019

Chris Porter, Natalie Trevaskis, Tim Quach, Sifei Han, Luojuan Hu, Enyuan Cao, Matthew Watt

Provisional

21 Oct 2019

Simon Corrie, Julia Walker, Kristian Kempe

European patent

29 Sep 2019

Vincent Jannin, Ben Boyd, Vithani Kapilkumar

UniSA

Provisional

4 Nov 2019

Azadeh Nilghaz, Ludivine Delon, Edward Cheah, Benjamin Thierry, Craig Priest

UQ Provisional 8 Nov 2019

Kris Thurecht, Vanessa Soh, Idriss Blakey, Muneer Ahamad, Ellen van Dam, Matt Harris

Provisional 8 Nov 2019

Kris Thurecht, Vanessa Soh, Idriss Blakey, Muneer Ahamad, Ellen van Dam, Matt Harris

Provisional 8 Nov 2019

Kris Thurecht, Vanessa Soh, Idriss Blakey, Muneer Ahamad, Ellen van Dam, Matt Harris

CBNS Annual Report 2019 37

E N GAG E M E NT

Patent applications, invention disclosures and selected commercial activities

Industry and commercial engagement CBNS researchers have a range of collaborations with commercial organisations. These relationships are typically led by one of our CIs and link with Australian and international biotech experts. The collaborations provide an opportunity for bio-nano research to be translated into product development and, ultimately, application and use. Examples include: Main Contact

Company

Content

Professor Kris Thurecht Aegros International

Smart affinity membranes for advanced manufacturing of high value therapeutic proteins

Professor Chris Porter

Capsugel

Ionic liquid technology for drug delivery

Professor Benjamin Thierry Professor Clive Prestidge

Ceramisphere Ltd. â&#x20AC;&#x201C; funded by an ARC Linkage grant

Development of an in vitro model of the intestinal epithelium to optimize oral nano-vaccine

Professor Ben Boyd Cerecin

Understanding dissolution, digestion and absorption of lipids from Cerecin proprietary formulations

Professor Andrew Whittaker

Dupont

Photo-responsive surfaces

Professor Benjamin Thierry

Ferronova Ltd.

Improving Head and Neck Cancer staging with nanoparticles

Professor Ben Boyd Galera Therapeutics

Understanding lipid and colloidal interactions with GC4419 and analogs

Professor Ben Boyd

3D Printing of Self-nanoemulsifying formulations

Gattefosse

Professor Chris Porter Halozyme Therapeutics

Perturbation of the extracellular architecture to promote the absorption and lymphatic transport of biological macromolecules

Professor Pall Thordarson

Precision Luminescent Solar Concentrators from Robust Quantum Dot Arrays

Lleaf Pty Ltd (ARC Linkage 2017-2020)

Professor Benjamin Thierry Murdoch University, WaterNSW, Seqwater and SA Water

Development and validation of rapid detection and viability assays for Cryptosporidium, Norovirus, and Adenovirus and using a novel gut-on-chip

Professor Justin Gooding

Nutromics Personalised Health

A wearable sensor for nutrition and wellness monitoring

Professor Clive Prestidge

Pharmako Biotechnology Pty Ltd Novel formulations for nutraceuticals

Professor Chris Porter

PureTech Health

Targeting the lymphatic system using glyceride-mimetic prodrugs

Professor Ben Boyd Sigrid Therapeutics

Understanding the effect of SiPore on lipid digestion and absorption

Professor Chris Porter Dr Angus Johnston

StarPharma

Novel targeted delivery systems

Professor Kris Thurecht

StarPharma

Imaging the distribution of dendritic nanomedicines

Professor Kris Thurecht Telex Pharmaceuticals

Biodistribution, pharmacokinetics and efficacy of therapeutic antibodies

Professor Pall Thordarson

Scaling manufacture of 3-D microstructures for the medical devices industry

Zhizhen Medical Co (ARC Linkage 2018-2020)

Further details on the range of industry and commercial engagements of CBNS can be found on our website: https://www.cbns.org.au/engagement/industrial-and-commercial-engagement/

38 CBNS Annual Report 2019

Health and medical sector engagement

Main Contact

Company

Content

Professor Stephen Kent

Aarhus University Denmark

Clinical trials of approaches to cure HIV

Professor Clive Prestidge Boulos & Cooper Pharma

Preclinical development of the novel antibiotic Ramizol

Professor Stephen Kent

Novel molecules to measure immune responses

Burnet Institute Melbourne

Associate Professor John McGhee Garvan Institute

Virtual reality cell explorer

Professor Clive Prestidge

Holman Pharma Pty Ltd

Improving oral delivery of poorly permeable drugs

Professor Stephen Kent

Imperial College London UK

Clinical trials of new HIV vaccines

Professor Andrew Whittaker

Institute of Nano Science and Technology, Mohali

Theranostic particles

Professor Andrew Whittaker Jilin University

Nanoparticles for imaging; fundamentals of polymer-Au NPs

Professor Benjamin Thierry

Massachusetts General Hospital & Harvard Medical School

Development of bioengineered in vitro tumour models for radiobiological studies

Professor Kris Thurecht

PA Hospital, Metro South, Brisbane

Brain Cancer

Professor Clive Prestidge

Reform Pharm Pty Ltd

Lipoceramic reformulation of cardiovascular drugs

Professor Benjamin Thierry Royal Adelaide Hospital

Smart “Nano Tracer” for improved preoperative staging with MRI and PET

Professor Benjamin Thierry

Royal Adelaide Hospital

Clinical trialling of Ferronva’s staging technology

Professor Andrew Whittaker

Shanghai Jiao Tong University

Iron Oxide NPs – responsive materials

Professor Andrew Whittaker

Singapore Bioimaging Consortium (A*STAR)

Cell uptake and imaging

Professor Benjamin Thierry

Stanford Cancer Institute

Development of nanomedicine molecular PEG tracer

Associate Professor John McGhee St Vincent’s Hospital Sydney

Pain management using virtual reality

Professor Stephen Kent

The University of California, Irvine

Novel influenza vaccine

Professor Kris Thurecht

University of Birmingham

Polymeric imaging agents

Professor Andrew Whittaker

University of California Santa Barbara

Block copolymer self asembly

Professor Stephen Kent

Vanderbilt University, USA

New antibodies to treat Influenza infection

Dr Adam Wheatley Vaxxas Professor Stephen Kent

Novel analyses on immune responses induced by nano patch

Further details on the range of industry and commercial engagements of CBNS can be found on our website: https://www.cbns.org.au/engagement/health-and-medical-sector-engagement/

CBNS Annual Report 2019 39

E N GAG E M E NT

CBNS researchers have a range of links with organisations in the health and medical sector. These relationships are typically led by one of our Chief Investigators and link with hospitals and medical research institutes. The collaborations provide an opportunity for bio-nano research to be undertaken in collaboration with medical researchers and practitioners, informed by end user needs.

CBNS Experience Aykut Zelcek’s research experience in Australia In March 2019, I had the chance to join CBNS as a visiting PhD student for a year. As a young researcher, I always knew that there were strong polymer chemists working in the field of nanomedicine and drug delivery in Australia. Still, I wouldn`t imagine that one day I will be a part of this highly intellectual atmosphere. While I was pursuing a PhD in my home country, I had an opportunity to continue my study abroad for a one year. With the orientation of my supervisor Professor Volga Bulmus and the help of CBNS Director Professor Tom Davis, I was able to get a scholarship and thereby my journey in Australia began. Under the supervision of Professor Davis, Dr Kristian Kempe and Dr Meike Leiske, I’ve worked on the design of polymer-based materials for siRNA delivery. During my study, I’ve had a chance to meet and work with other CBNS students and researchers in a welcoming atmosphere. With the invitation of Professor Davis, I’ve joined NHMRC grant workshops held in Sydney where I’ve had an opportunity to present my work and meet our collaborators, Associate Professor Joshua McCarroll and CBNS CI Professor Maria Kavallaris AM. These meetings have given me the chance to introduce myself, grow my network and hear about other studies going on in the field. Rapidly I’ve become a part of a highly collaborative environment and met pioneer researchers. Thanks to remarkable research facilities and experienced researchers in CBNS, I was able to carry on various experiments in different biology and chemistry labs and had lots of hands-on experience. I believe that my study in Australia has led to increased development of my personality, both socially and scientifically and given me unforgettable memories.

Aykut Zelcek with colleagues

40 CBNS Annual Report 2019

Joanne Ly with colleagues

Alumna Joanne Ly’s Experience Dr Joanne Ly completed her PhD with Centre Director Professor Tom Davis at the Monash University node of CBNS in 2019, researching novel nanomaterials for selective magnetic resonance imaging (MRI).

Early 2019, Joanne moved from Melbourne to Canberra, starting her new job at IP Australia and writing her PhD thesis. She had finished all lab work and was able to submit not long after moving to Canberra.

During her third year of her PhD Joanne started applying for jobs to gather interview experience and to explore opportunities outside of academia. After attending a CBNS IP Workshop, Joanne decided to apply for a role as a Patent Examiner at IP Australia and was successful.

Joanne’s team examines patents relating to polymers and applied chemistry.

IP Australia is a federal government agency that administers intellectual property rights for patents, trademarks, designs and plant breeder’s rights in Australia.

“Some cases I have examined include sponge cakes, dog food, pesticides, polymeric drug delivery, catalysts, security pigments, cardboard, porous silica particles and membrane detergents and alcoholic ice cream!” Joanne is currently in her final stage of training and really enjoys her job. “Surprisingly, I enjoy learning about patent law, but the best part of my job is that every day I learn a newinteresting fact!“

Events Events provide an excellent opportunity to share important research, form new collaborations and strengthen existing ones. CBNS delivered another series of successful events during 2019. Here are some of the highlights.

CBNS Annual Research Workshop

E N GAG E M E NT

The 2019 CBNS Annual Research Workshop took place at the RACV Healesville Country Club in Victoria from 27 to 29 November 2019. With lovely weather and eager brains, this year’s workshop was a huge success. Over 100 delegates shared their research achievements and challenges they faced during 2019. Each CBNS node presented updates on their current work, the life at their respective nodes and discussed future research opportunities. Furthermore, a special mentoring event enabled CBNS members to ask probing questions and engage in discussions with experienced and successful scientists from inside and outside academia. There was the opportunity to talk with other members or CIs about ideas and potentially start new collaborations towards new career paths. We recognised members for the most significant publication of 2019, the best poster presentation as well as the winner of the Science by Design Competition. The workshop also included recognition of the 2019 CBNS Student Travel Award and the inaugural Bio-Nano Travel Grant winners (see page 51 for details). A joint meeting of the CBNS Education Committee, the Outreach Sub-Committee and the Industry Partners Program Committee facilitated discussions and feedback for events, training and activities that could take place during 2020. All committees’ programs and activities during 2019 were positively received by members at the workshop. This has resulted in a range of beneficial activities that not only build the capacity of CBNS members but also provide a platform to demonstrate the centre’s important work to the public.

International Nanomedicine Conference The CBNS was once again the co-host with UNSW of the Australian Centre for Nanomedicine’s International Nanomedicine Conference. This was the 10th annual conference to be held, with a change of venue to Pier One, Sydney Harbour. The conference brought together Australian nanomedicine researchers from medicine, chemistry, engineering and sociology, with international experts. A feature each year is the opening plenary talk delivered by the CBNS Visiting Professor. In 2019, Professor Chunying Chen of the National Center for Nanoscience and Technology in China presented on the Crown and the Scepter: Roles of the Protein Corona in Nanomedicine. In addition to the talks and poster sessions, the conference is a confluence of national and international expertise and most CBNS researchers attended. The CBNS Scientific Advisory Board meets during this event, with expert input and advice from the plenary speakers each year.

CBNS Annual Report 2019 41

Professor Chunying Chen with colleagues

2019 Visiting Professor: Chunying Chen Our annual Visiting Professor Program brings outstanding international scientists to Australia to advance bio-nano science. In 2019, Professor Chunying Chen, Principal Investigator at the Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology in China visited Australia. Professor Chen works as as a group and project leader at the China Nanosafety lab. Professor Chen has been awarded the National Award for Innovation and Outstanding Service to the Standard authorised by Standardization Administration of the Peopleâ&#x20AC;&#x2122;s Republic of China in 2011, the Second Prize of Beijing Science and Technology (ranked second) in 2008, the Second Prize of the National Natural Science Award (ranked second) in 2012. Professor Chen visited Australia in two short trips; in June 2019, for the International Nanomedicine Conference and a visit to UNSW. She returned in November attending the CBNS Annual Research Workshop where she delivered a research presentation and met with CIs, ECRs and students. As part of the Visiting Professor program, Professor Chen participated in the CBNS Scientific Advisory Board. The Visiting Professor in 2020 will be Associate Professor David Tai Leong from the National University of Singapore.

42 CBNS Annual Report 2019

Media presentation workshop

ARC Joint Media and Presentation Training Twice in 2019, CBNS organised a joint workshop for members of ARC Centres to develop confidence and practical skills for interviews with the media, public speaking and the general public. It was designed to help ECRs and students to acquire the skills to get their research message across a wide range of audiences. A special feature of the workshop was the collaboration of very different research fields simulating interviews on their respective research in mixed working groups. The learnt skills are also valuable in preparing for job interviews, fellowship applications, delivering presentations or even writing articles. The workshops were a huge success for all Centres with new collaborations forming and interdisciplinary networks developing.

Our first 2019 workshop, held in late February, included CBNS, the Centre of Excellence for Mathematical and Statistical Frontiers (ACEMS), and the Centre of Excellence for Climate Extremes (CLEX). A great success with 100% of participants in the workshop agreed they achieved their goals. Expanding further, our August workshop incorporated The Centre of Excellence in Population Ageing Research (CEPAR) and Centre of Excellence for Quantum Computation and Communication Technology (CQC2T).

Frontiers in Bio-Nano Science

FBNS was created to support final year PhD students and EMCRs in crossdisciplinary networking and provide a unique environment for attendees to discuss big picture ideas in research and industry and gain insight on how to translate their ideas and research outcomes into innovation and market. The interactive program allows for attendee participation and frequent engaging discussions. Two plenary speakers provided unique viewpoints from two different backgrounds. The first plenary session was an inspiring talk by Queensland Chief Entrepreneur, Ms Leanne Kemp about challenges associated with translating cutting-edge technologies to applications and achieving entrepreneurship skills. The second plenary featured Professor Alan Rowan, Director of The Australian Institute for Bioengineering and Nanotechology (AIBN) based at UQ and Chair of the CBNS Scientific Advisory Board, an expert leader in research and academia.

Six poster presentations took place during the lunch break allowing attendees to learn about further research and network with speakers and other FBNS delegates. The next part of the event was an engaging and interactive panel discussion on “Benchtop to the market place” which fostered positive discussion and involvement with EMCRs. The five expert panellists from varying backgrounds in academia, industry, business and start-up included:

E N GAG E M E NT

After a successful Frontiers in Bio-Nano Science (FBNS) Symposium in 2018, CBNS and BLiSS held the early to mid-career researcher (EMCR) event in October 2019 at The Precinct in Fortitude Valley, Brisbane.

• Professor Maree Smith AC; Director, Centre for Integrated Preclinical Drug Development (CIPDD), School of Biomedical Sciences, UQ • Dr Peter Devin; Chief Executive Office, Uniseed • Mr Angus Forster; Chief Development and Operation Officer, Vaxxas Pty Ltd • Dr Carrie Hilyard; Chair, Fitgenes Australia Ltd-Deputy Chair, Mater Research Institute • Dr Doug Horton; Principal at Spruson and Ferguson-Director of IP management, UniQuest

An experienced committee facilitated two informative oral presentation sessions with eight presentations from selected CBNS Members around two themes: “Not so Basic Science”, and “Collaboration is Key”. The presentations were followed by panel questions, discussion and engagement of new ideas.

CBNS Annual Report 2019 43

One of the highlights of the day was “Spark Tank”, where five speakers each delivered a five-minute business pitch of their innovative research ideas, which were judged by three experts from academia, a venture capital firm and research commercialisation.

oral presenters, securing sponsorship prior to the event and facilitating the symposium on the event day.

At the end of the formal program, participants had the opportunity to apply their knowledge from the day and network with seven innovators categorised in four groups:

Dr Sohinee Sarkar – Chair (BLiSS) Dr Pradeep Rajasekhar – Program Co-Chair (Monash) Ms Ava Faridi – Communications Co-Chair (Monash) Mr Nicholas Westra Van Holthe – Operations Co-Chair (UQ) Ms Samantha Snowden-Finch – Lead Coordinator (CBNS) Ms Anne Meyer – Lead Coordinator (CBNS)

• Professor Michael Good (Academia) • Mr Tim Macuga & Ms Madeleine Kersting Flynn (Communication) • Professor Darren Martin & Ms Sonja Plompen (Commercialisation and industry) • Mr Douglas Bent & Dr Ben Finnin (Industry-sponsors) The overall symposium was very well received by delegates, invited speakers, panellists, innovators and sponsors with highly positive feedback from various groups. The different format allowed the event to be more relaxed and resulted in more engaging scientific conversations. Organising FBNS 2019 provided an opportunity for active CBNS members to achieve and improve their event management and team-work skills through responsibilities for designing and planning the program, inviting speakers, coordinating a workshop for

44 CBNS Annual Report 2019

The event would not have been possible without the hard work of: Chairs and Coordinators

Committee Members Ms Salma Ahmed Dr Dewan Akhter Dr Craig Bell Ms Caterina Brighi Ms Bruna Cambraia Garms Dr Changkui Fu Ms Anna Gemmell Dr Zil E Huma Dr Manasi Jambhrunkar

Dr Aleksandr Kakinen Ms May Lai Dr Meike Leiske Ms Ayaat Mahmoud Dr Azadeh Nilghaz Dr Paulina Ramírez García Dr Kristel Tjandra Ms Adil Usman Ms Cindy Xiao

We thank the sponsors for supporting the event, travel bursaries and prizes.

Education and Training Committee The Education and Training Committee’s objective is to identify the training and capacity building requirements of the members of the Centre. In a collaborative, inclusive and transparent manner, the Committee designs, promotes, delivers and evaluates an annual series of education-based events.

The CBNS Education and Training Committee met monthly during 2019 to assess and organise the training and education needs of the Centre.

Storytelling Targeting EMCRs and late-stage PhD students, this six-month training program assisted participants in learning how to influence, engage and inspire with stories. The program comprised a oneday workshop and an on-going practice program. The training aimed to improve communication and presentation skills. Participants were taught how they could tap into the natural power of storytelling in a pragmatic and authentic way. The one-day workshop was highly interactive and participants engaged in hands-on activities designed to build their skills and knowledge. Delivered online, over six months, the Deliberate Practice Program involved one practical activity each week, helped participants practice their new skills in the workplace.

2019 Education and Training Committee events: Event title

Date

Node

ACEMS, CBNS and CLEX Media & Presentation Training*

28 Feb

Monash

Storytelling for Leaders

2 Apr

Monash

Illustrator Workshop 15-16, 18-19 Jul

Monash and UQ

ACEMS, CBNS, CEPAR, CLEX and CQC2T Media & Presentation Training*

23 Aug

UNSW

Fiji/Image J Workshop

10-11 Sep

Animal Imaging Workshop

12-13 Sep

Frontiers in Bio-Nano Science

25 Oct

Biomedical Applications of Engineered Antibodies and Proteins

18 Nov

Monash

*Joint Education and Outreach event

E N GAG E M E NT

The Education and Training Committee is chaired by CBNS CI Professor Maria Kavallaris AM (UNSW). The committee is formed of members from across all nodes with each node being represented by a Postdoctorate researcher and a PhD candidate.

Illustrator Workshop Nowadays, a researcher can no longer rely on the power of their words to convey a convincing scientific argument – information in the form of diagrams plays just as important a role. Being confident in one’s own ability to produce such diagrams is a fundamental skill. This two-day workshop was specifically designed for scientists to help them understand basic graphic design software Adobe Illustrator. During the training, participants developed skills using this software through step by step learning and practice-based projects.

Illustrator workshop

FIJI/Image J Workshop A two-day workshop that introduced digital imaging and basic navigation in Fiji/Image J. The training covered basic manual analysis such as cell counts, area, segmentation and tracking, as well as how to automate previously collected analysis using programming and scripting.

Animal Imaging Workshop Over two days, participants had the opportunity to focus their time on imaging practices. The workshop covered the theory, practical, analytical information and hands-on practice needed for doing the imaging experiments with the aim of preparing the participant for future imaging studies. Storytelling program

CBNS Annual Report 2019 45

Mentoring Program Mentoring relationships support the professional and personal growth of mentees through the development of new skills, finding new approaches to old problems, identifying areas for professional growth and accessing a wider professional network. These strategies are provided through the guidance or sponsorship offered by a mentor. Similar to previous years, mentors are chosen and matched with mentees based upon their relevant skills, knowledge, experience or influence. Through the mentor-mentee relationship, the mentor may assist the mentees in developing future career goals and defining a framework to assist the mentee in reaching those goals. Mentoring can be beneficial in skill development, career and goal planning, developing successful networks,

46 CBNS Annual Report 2019

confidence building, developing managerial/leadership capabilities and understanding organisational structures. The CBNS-led mentoring program is a formal mentoring arrangement and is designed for Early Career Researchers (ECR) and Mid-Career Researchers (MCR). The mentor-mentee relationship may last through the progression of the mentees current postdoctoral research project. Through the development and progression of the mentorship, the interaction can either focus on a particular challenge or concern, or on broad career questions. The agenda is led by the mentee â&#x20AC;&#x201C; the mentor is there to facilitate, not influence decisions. The CBNS continued with the same mentoring team as in 2018 to review the applications and try and match each with a mentor â&#x20AC;&#x201C; either from within the CBNS or external. The CBNS

Mentoring Team is supported by CBNS CIs Professor Maria Kavallaris AM (UNSW), Professor Stephen Kent (UoM) as well as the Chief Operating Officer Dr Natalie Jones. While CBNS postgraduate students are not formally part of the mentoring program, support for students is provided by resources available at each node or institution. The Centre encourages students to interact with senior research leaders and ECRs at their node and more broadly with CBNS members through regular research and training opportunities. In 2019, the Centre offered Mentoring Circles as part of their Annual Research Workshop. This provided a larger group of mentees with an informal opportunity to ask mentors questions relating to their career path or their research.

Industry Partners Program Committee The Industry Partners Program (IPP) identifies and creates opportunities to engage CBNS research with industry and commercial stakeholders. The program recognises the diverse opportunities available to Centre members within and outside academia and explores methods to engage and showcase CBNS activities to end-users of bio-nano science and technology. The committee is chaired by CBNS CI Professor Kris Thurecht (UQ) and includes members from all nodes, including a PhD candidate and a Postdoctorate researcher. The committee was created in June 2019 and met quarterly to discuss and coordinate industry engagement activities, build networks, expand the mentoring program and support ongoing relationships and communication with industry and end-users.

IPP Industry Workshop

E N GAG E M E NT

The IPP Committee held their first activity, an industry workshop, in November 2019. The workshop objectives revolved around discussing some of the challenges and the opportunities of working in, and with industry and how to pursue a career in industry. Speakers were invited to talk about building successful research, careers and networks. CBNS was fortunate to have speakers from BioCurate, CSIRO, IP Australia, Pfizer and Starpharma representing industry, academia and government. Speakers enlightened members with their own diverse career stories. Held at Monash, the workshop drew CBNS members from all five nodes.

CBNS Annual Report 2019 47

Translation Strategy and Industry Collaboration Toolkit The purpose of the Translation Strategy is to outline pathways for promoting CBNS research and translation efforts, and identify potential routes for implementation of research outcomes. The Industry Collaboration Toolkit provides research staff and students with avenues to explore the process for creating collaborations with pharmaceutical industry companies, academia and government, tapping into the resources and contacts available at each of the CBNS nodes.

IPP Translation Strategy

Stage 1:

Stage 2:

Research What is your research? Start with the fundamental research Build on foundations with applied research

Technology discovery Is your research innovative? Must be new, inventive and useful Define the core opportunity Complete an invention disclosure form

Stage 4:

Stage 3:

Build the asset/Product development Have you developed your research to meet marketing requirements? Secure the IP (e.g. patent) Development plan, marketing plan and materials Commercialisation strategy and protection strategy

Assessment Have you defined the value of your research in the market? Intellectual property position â&#x20AC;˘ Prior disclosure â&#x20AC;˘ Ownership Establish market opportunity in a competitive landscape Understand the value proposition Identify funding assistance opportunities

Stage 5:

Stage 6:

Approach industry What is your pitch to industry? On-going marketing Identify and discuss with potential partners

Secure the deal Have you started negotiations to formalise an agreement? Negotiations and draft agreement Execute the deal â&#x20AC;&#x201C; start up, licence, option, or assignment

Stage 7: Post deal Have you defined milestones and distribution of revenue? Establish research and commercial milestones Agreement compliance Commercialisation revenue distribution

48 CBNS Annual Report 2019

Outreach Sub-Committee The CBNS Outreach Sub-Committee is a sub-committee of the CBNS Education and Training Committee. It is chaired by CBNS CI Associate Professor Matthew Kearnes (UNSW). The Committee is formed of members from across all nodes with each node being represented by a Postdoctorate researcher and a PhD candidate. They met monthly during 2019 to assess the outreach needs of the Centre and coordinated a series of events across CBNS nodes. The Outreach Sub-Committee identifies and promotes CBNS research to the public. It achieves this through community or industry-related series of outreach activities, events or media liaison. This includes working with the CBNS Education and Training Committee and the IPP Committee to ensure strong collaboration and synergies occur across CBNS programs

MPavilion 4 Feb

Monash & UoM

ACEMS, CBNS and CLEX Media & Presentation Training*

28 Feb

Monash

Pint of Science Brisbane

20 May

MPavilion

Pint of Science Melbourne

21 May

UoM

The first CBNS public outreach event of 2019 was MPavilion. A free series of talks, meets, music, shows and programs held in an architecturally awarded and designed pavilion in the Queen Victoria Gardens, Melbourne. CBNS participated as part of the STEM program in partnership with the Office for Women. Our volunteer researchers showcased the centre of a cell through a VR experience; demonstrated how you might use metal phenolic networks as ink; and participated in an informative panel as part of a Q&A session with the audience.

Extrasensory – National Science Week 10 Aug

Monash & UoM

Primary School Science Fete – National Science Week

14 Aug

UNSW

To Bio-Nano & Beyond – National Science Week

16 Aug

UniSA

Brisbane Science Festival – National Science Week

17-18 Aug UQ

ACEMS, CBNS, CEPAR, CLEX and CQC2T Media & Presentation Training*

23 Aug

UNSW

STANSW Chem STEM 2019 (Chemistry Teachers Conference)

23 Sep

UNSW

CBNS members involved in MPavilion were Dr Kye Robinson, Ms Julia Walker, Dr Meike Leiske, Dr Simona Carbone,

2019 Outreach Sub-Committee events: Date

Node

*Joint Education and Outreach event

Dr JJ Richardson, Dr Matt Faria, Mr Edward Henderson, Ms Vidhishri Kesarwani, Ms Ayaat Mahmoud and Mr Gabriel Huynh.

Pint of Science Two CBNS members presented their research at the Pint of Science 2019 event. Pint of Science brings some of the most brilliant scientists to local pubs to discuss their latest research with an interested audience. No prior knowledge is needed and it is a valuable chance to meet the people responsible for the future of science. In Melbourne, Dr JJ Richardson (UoM) spoke on ‘Iron-Man’ suits on the nanoscale, at The Provincial Hotel, Fitzroy. In Brisbane, Dr Zach Houston (UQ) entertained audiences on The future of personalised cancer treatment in his talk at the Newstead Brewing Co., Newstead.

Pint of Science CBNS at MPavilion

CBNS Annual Report 2019 49

E N GAG E M E NT

Event title

National Science Week National Science Week (NSWk) is Australia’s annual celebration of science and technology providing an opportunity to acknowledge the contributions of Australian scientists to the world of knowledge. It also aims to encourage an interest in science pursuits among the general public and to encourage younger people to be fascinated by the world we live in. 2019 was host to over 2070 events. CBNS kicked off NSWk activities with Monash and UoM nodes participating in Extrasensory on Saturday 10 August. The Parliament of Victoria and The Royal Society of Victoria played host to 13 exhibits and numerous shows and workshops with an evening of sensory exploration. CBNS ran the VR experiences to great success. Over 1000 members of the public braved a Melbourne winter’s night to Journey to the Centre of the Cell. Volunteer members included Arisbel Batista Gondin, Ayaat Mahmoud, Edward Henderson, Hannah Kelly, James Grace, Paulina Ramírez García and Vidhishri Kesarwani. UNSW was home to the Primary School Science Fete on Wednesday 14 August. Spread over two sessions, 160 primary school children were able to explore various sciences. Chelsea Forest and Kristel Cahyadi Tjandra assisted the enthusiastic Primary School students in learning what the surface and the inside of a cell looks like.

To Bio-Nano and Beyond

Two days later on Friday 16 August, more than 50 high school children from six schools descended on UniSA. A prodigious squad of UniSA members ran the CBNS/UniSA only event with five activities delving into the nanoworld. A tremendously well planned and executed day, with media interest, left the teenagers with an open mind To Bio-Nano and Beyond. A large group of members volunteered and made this day run seamlessly: Amalie Moller, Anita Kral, Anthony Wignall, Aurelia Elz, Azadeh Nilghaz, Benjamin Thierry, Campbell Strong, Chelsea Thorn, ChihTsung Yang, Clive Prestidge, Desmond Williams, Douglas Howard, Duy Phu Tran, Edward Cheah, Eve Nimtrakul, Fitore Hoxha, Hayley Schultz, Kristen Bremmel, Leah Wright, Luke Bredberg, Manasi Jambhrunkar, Marnie Winter, Meysam Rezaei, Michelle Maritz, Mohamed Amen, Mona Elsemary, Muhammed Awad, Nghia Pham, Nicky Thomas, Parisa Badiee, Pouya Dehghankelishadi, Ruba Almasri, Saji Maghrebi, Santhni Subramaniam, Steph Hodby, Tahlia Meola, Tim Barnes, Yassamin Albayaty and Zhaobin Guo.

Over a beautiful sunny weekend, CBNS finished off NSWk at the Brisbane Science Festival. With more than 4000 people through the gates across the two days, keeping up with the excitement and enthusiasm from the public ensured all our experiments were fully utilised over the festival. Volunteering members included: Adil Usman, Amber Prior, Anna Gemmell, Arunpandian Balaji, Bruna Cambraia Garms, Caterina Brighi, Craig Bell, Dewan Taslima Akhter, Felicity Han, Francis Mccallum, Gayathri Ediriweera, James Humphries, Jennifer Schoning, Jose Pablo Parra Pasten, Merryn Strange, Nick Westra Van Holte, Phillip Janowicz, Sachini Fonseka, Salma Ahmed, Vanessa Soh, Weizhi Xu, Zach Houston and Zherui Xiong.

Brisbane Science Festival

Extrasensory

UNSW Primary School Science Fete

50 CBNS Annual Report 2019

Brisbane Science Festival

CBNS Awards During 2019, members at all levels were recognised for their contribution to the Centre, as well as their hard work and dedication to science and pushing the boundaries of understanding in the area of bio-nanoscience. This section includes some of the highlights. See page X-X for a comprehensive list. During the CBNS Annual Research Workshop (ARW) held from the 27 to 28 November 2019, we recognised members for the most significant publication of 2019 and best poster presentation at the workshop as well as the winner of the Science by Design Competition. Furthermore, one student from each node was presented with the 2019 CBNS Student Travel Award and the newly created CBNS Bio-Nano Travel Grant, a travel grant for postdoctoral researchers. Congratulations once again to the following awardees:

Most significant CBNS paper of 2019 (Postdoc)

Most significant CBNS paper of 2019 (Student)

Dr Angel Tan (Monash)

Mr Ibrahim Javed (Monash)

Probing cell–nanoparticle (cubosome) interactions at the endothelial interface: do tissue dimension and flow matter? (Biomater. Sci., 2019,7, 34603470)

Inhibition of amyloid beta toxicity in zebrafish with a chaperone-gold nanoparticle dual strategy Nature Communications volume 10, Article number: 3780 (2019) ARW Best poster presentation of 2019

Ms Aria Ahmed-Cox (UNSW)

2019 CBNS Student Travel Awards:

Ms Ava Faridi (Monash)

Mr Yutian Ma (UoM)

Ms Chelsea Forest (UNSW)

Science by Design Competition 2019 (Judge’s and People’s Choice Award winner)

Mr Nghia Pham (UniSA)

Mr Meysam Rezaeishahmirzadi (UniSA) represented by Dr Marnie Winter Ms Caterina Brighi (UQ)

2019 CBNS Bio-Nano Travel Grant:

For a comprehensive list of awards, memberships and grant successes, see pages 67-69. Dr Laura Fitzgerald (Monash)

Dr René Lafleur (UoM)

Dr Rowan Hughes (UNSW)

Dr Azadeh Nilghaz (UniSA)

Dr Changkui Fu (UQ) CBNS Annual Report 2019 51

E N GAG E M E NT

Visualisation and quantification of nanoparticle uptake in 3D tumour models

CBNS Podcasts Small Talk with CBNS CBNS utilises a diverse range of opportunities to communicate about Centre-led research, and inform on exciting discoveries in bio-nano science. 2019 saw the release of the first series of CBNS podcasts: Small Talk with CBNS (Small Talk). Small Talk was created to delve into the minds of renowned researchers in the fields of bio-nano science and technology, and provide an insight into how experienced researchers have chosen their career. The podcasts are short (30 mins), informative and entertaining. In this first series, we interviewed CBNS Visiting Professor, Professor Warren Chan from the Institute of Biomaterials and Biomedical Engineering at the University of Toronto who spoke in great length about the history and the future

52 CBNS Annual Report 2019

of nano from a medical and technical sense. Further interviewees involved Centre Director Professor Tom Davis, CI and UNSW Node Leader Professor Justin Gooding and CI Associate Professor Matthew Kearnes. Members maximised this opportunity with involvement in the planning, recording, interviewing and editing. Thank you to Dr Simona Carbone, Dr Paulina Ramirez Garcia, Dr Daniel Yuen, Dr Simon Corrie, Dr Orlagh Feeney and Dr Laura Fitzgerald.

The podcasts were released each month singularly from July to November coinciding with the CBNS electronic newsletter. They are available to listen to on our CBNS website.

SMALL TALK WITH THE CBNS

Media Coverage Media coverage of CBNS provides the public with important insights into the diverse range of research being undertaken at the Centre. It highlights the Centre’s contribution in understanding the interface between nanoengineered materials and biological systems, and translating these discoveries into better methods, tools and technologies. This coverage also gives Centre members a key opportunity to contribute to public debates on science and to facilitate increased understanding of the importance of scientific progress.

Detailed on this page are four stories that garnered considerable media interest through television, major and regional newspapers. A breakthrough for CBNS researchers Dr Ibrahim Javed and Centre Director Professor Tom Davis, both of Monash University, where combining milk protein with gold was successful in curing zebrafish of Alzheimer’s disease. Having used the milk protein-coated gold particles to cure zebrafish suffering a form of dementia, Professor Davis said the development was continuing to see if it could eventually be suited to humans. “It is almost like taking a statin where you take them every day and, if you did have these things building up in your brain or other parts of your body, they would be removed.” Working with colleagues in the US and China, CBNS scientists found a milk protein called β casein had properties almost identical to the Alzheimer’s disease-causing plaque but is non-toxic. Because they were so similarly matched, lead researcher Dr Ibrahim Javed said the drug was able to bind with the toxic plaque before it was excreted by the body. “We found that it completely restored the symptoms. The treated fish were acting exactly like the untreated control fish,” Dr Javed said. “The β casein prevented the plaque formation and then it cleaned up the brain. Zebrafish share more than 90 per cent of their genes with humans and are transparent, making them strong candidates for research into brain diseases. Published in the Herald Sun on 24 August 2019.

109

51,462

newspaper or magazine articles

media releases

page views on website

Front page of the Herald Sun on the 26 June 2019 saw a piece about CBNS researchers having developed a disposable device that can warn doctors of dangerous birth complication; it hopes to save many of the 500,000 babies who die each year from pre-eclampsia. The new test cannot only diagnose pre-eclampsia within half an hour but also uncover the severity of the condition even in remote parts of the world. In recent years the breakthrough discovery of two markers in the blood of pregnant women paved the way for a hospital-based test capable of diagnose the dangerous condition in about 24 hours. CBNS and UniSA researchers have now built upon that work, developing a cheap handheld device that can quickly identify levels of the same placental growth factor makers. “With pre-eclampsia, you really want to pick it up very quickly because a 24-48-hour delay really can have quite serious consequences,” CI Professor Benjamin Thierry said. “We know the detection works, we get the response very quickly, but it is how you implement the engineering so that it can work in remote environments.

November 2019 saw the release of a Nature Nanotechnology paper from Dr Paulina Ramírez-García and Dr Nicholas Veldhuis which gained nationwide publicity including the Herald Sun and Channel Seven News (5 November 2019). Their research involved increasing the efficacy of existing pain killers. They created a nanoparticle drug carrier, a sneaky way of packing a microscopic capsule – 1,000 times thinner than a human hair – with a pain reliever that can interrupt the pain signal from within the cell. With millions of people suffering chronic pain; there was an urgent need to find more effective and safer treatments. “Every single one of the analgesics we use to treat pain has associated side effects,” Dr Veldhuis said. “New drug candidates have been identified, but they haven’t made it to the clinic. “One of the key questions we hoped to address was; is the drug getting to the right location? If it’s not, can we use nanotechnology to deliver drugs to a specific place to get a better outcome?”

Televised through Channel Seven and Channel Nine on 15 August 2019, CI Professor Clive Prestidge and his team from UniSA, including PhD student Ms Santhni Subramaniam, spoke about successfully using microscopic nanoparticles to carry antibiotics to hard-toreach infected cells. They expected the method to work just as well on urinary tract infections, bone and wound infections. “Over time, bacteria have learnt how to hide in human cells, making some diseases resistant to antibiotics. To get around this challenge we have put the antibiotic into a very, very small vehicle that enables it to go into the body and kill the invading bacteria hiding in the cells,” Professor Prestidge says. The nanoscale capsules containing the antibiotics are eaten by the infected cells, which then die at a much higher rate.

CBNS Annual Report 2019 53

E N GAG E M E NT

2019 was a bustling year for CBNS. A number of significant papers were published which resulted in increased engagement by the media. Published papers reflect the success of the Centre and give CBNS members the opportunity to expand their network, promote their ideas within the scientific community and refine their research.

54 CBNS Annual Report 2019

G OVE R NAN C E

Governance CBNS Annual Report 2019 55

Governance and Management The CBNS Governance Board has an independent advisory role to the Centre Director and Centre Executive and provides guidance on governance, strategy and stakeholder engagement. The CBNS Scientific Advisory Board provides strategic scientific insight and commercial direction to the Centre. The CBNS Executive meet regularly to discuss the research program, operational matters and Centre finances.

Executive Committee

Scientific Advisory Board

Governance Board

Director: Professor Tom Davis Deputy Director: Professor Frank Caruso

Chair: Professor Alan Rowan Centre Manager Dr Natalie Jones

Chair Education and Training Program

Chair Outreach Program

Chair: Professor Peter Doherty AC Chair Industry Partners Program

Centre Administration

Education and Training Program Chair Professor Maria Kavallaris AM

Monash node

UoM node

UQ node

UNSW node

UniSA node

Node Leader Professor Tom Davis

Node Leader Professor Frank Caruso

Node Leader Professor Andrew Whittaker

Node Leader Professor Justin Gooding

Node Leader Professor Benjamin Thierry

Outreach Program Chair Associate Professor Matthew Kearnes

Industry Partners Program Chair Professor Kris Thurecht

Research Program Research Themes Delivery Systems and Vaccines

Sensors and Diagnostics

Imaging and Visualisation

Overarching Projects/Signature Projects Chief Investigators, Co-lead Investigators, Postdoctoral Researchers, Research Staff, Research Students

56 CBNS Annual Report 2019

Strategic Fund

Governance Board The CBNS Governance Board Members are all experts and leaders in research and governance in scientific, research-based organisations. The Board monitors progress towards delivery of Key Performance Indicators and approves research, operational and financial plans. The Governance Board met twice in 2019: on 18 April and on 15 August.

Professor Peter C. Doherty AC

A graduate of the University of Queensland School of Veterinary Science, Professor Doherty shared the Nobel Prize in medicine 1996 for his immunology research and was the Australian of the Year in 1997. Since then, he has gone in to bat for evidence-based reality, relating to areas as diverse as childhood vaccination, global hunger and anthropogenic

climate change. So far, he has published 5 “lay” books on science with the latest, “The Knowledge Wars”, suggesting how those who don’t know much (or anything) about science can “interrogate” both the scientific evidence and those who claim to be scientists for themselves.

Professor Calum J. Drummond

Professor Drummond is currently Deputy ViceChancellor Research and Innovation and Vice President at RMIT University, playing a leadership role in the development of discovery and practicebased research and in building and enhancing capability in research and innovation across the University. He is also an active research professor and has published over 200 papers and patents in the area of advanced materials, including biomedical and energy storage applications.

Professor Drummond joined RMIT University in 2014 from CSIRO where he was Group Executive for Manufacturing, Materials and Minerals comprising 1300 researchers and research support staff. Earlier he was seconded from CSIRO to be the inaugural Vice President Research at CAP-XX, an Intel portfolio company that developed supercapacitors for consumer electronic products.

Ms Maureen O’Keefe

Ms O’Keefe has spent her career in higher education, research and health organisations and has more than fifteen years’ experience in senior executive roles. She is a Board member of Vision2020 Australia, the BioMelbourne Network and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology Governance Board. Previously, Ms O’Keefe was a member of the Council of the Victorian Cancer Agency for 6 years, a Ministerial appointment, including two years as a member of the VCA Clinical Trials Working Group and more recently a Ministerial appointment on the Victorian Department of Health’s Clinical Trial Research Consultative Council.

Ms Filippa Shub

Ms Shub has more than 25 years’ experience in the biotech and pharmaceutical industry working in senior roles in General Management, Commercialisation, Intellectual Property and Research Operations. Ms Shub is currently Director of IP and a member of the executive management team of ASX listed Starpharma. Previously, she has

worked with both legal firms and biotechnology companies in Australia and USA, including Davies, Collison Cave, Allens Arthur Robinson, Norwood Abbey and CSL. In addition, Ms Shub co-founded a clinical research company in the US. She is a member of the AusBiotech IP expert panel and was previously Vice President of a school board.

CBNS Annual Report 2019 57

G OVE R NAN C E

Ms O’Keefe was appointed as Chief Executive Officer of the Australian College of Optometry in March 2013, an organisation providing public health eye care, tertiary clinical teaching and education, and research to preserve sight and prevent blindness. She spent the previous seven years as Chief Operating Officer at the Walter and Eliza Hall Institute of Medical Research and prior to that, held several senior executive roles at the University of Melbourne. Ms O’Keefe is a graduate of the Australian Institute of Company Directors, the Williamson Community Leadership Program and an Executive Education Program at Massachusetts Institute of Technology, Cambridge.

Dr Warwick Tong

Dr Tong is currently Chair of the CTxONE Pty Ltd, a spin-off company from the Cancer Therapeutics Cooperative Research Centre headquartered in Melbourne. He is also Chair of the Board of ASXlisted Amplia Therapeutics, a member of the Scientific Advisory Board of the Maurice Wilkins Centre in Auckland NZ, the Advisory Board of Cortex Health, Melbourne, the Industry Advisory Board, School of Biomedical Sciences, University of Melbourne and a member of the CSIRO Manufacturing, Business Advisory Committee. He has spent more than 27

years in executive management in drug development and commercial roles in both the major pharmaceutical and biotech industry. After graduating as Senior Scholar in Medicine from Auckland University and working in General Practice Dr Tong joined Glaxo in NZ as Medical Director and subsequently worked in Singapore and London, in regional and global business development and commercial roles for Glaxo. Prior to coming to Melbourne, Warwick spent five years in Boston as SVP, Development, for Surface Logix Inc.

Professor Gordon Wallace (AO)

Professor Wallace is currently the Executive Research Director of the ARC Centre of Excellence for Electromaterials Science and the Director of the Australian National Fabrication Facility, Materials Node. Professor Wallace is a Fellow of the Australian Academy of Science, the Australian Academy of Technological Sciences and Engineering (ATSE), the Institute of Physics, the Royal Australian Chemical Institute (RACI) and the Royal Society of NSW. He is a corresponding member of the Academy of Science in Bologna. On 26 January 2017, he was appointed as an Officer of the Order of Australia. Furthermore, he received Wollongongâ&#x20AC;&#x2122;s award for Innovation in 2017, served as Wollongongâ&#x20AC;&#x2122;s Australia Day Ambassador and was named NSW Scientist of the Year 2017. He received the Eureka Prize for Leadership in Science and Innovation in 2016. In 2015, he was appointed to the Prime Ministers Knowledge Nation.

58 CBNS Annual Report 2019

Professor Wallace is involved in the design and discovery of new materials for use in Energy and Health. In the Health area, this involves using new materials to develop biocommunications from the molecular to skeletal domains in order to improve human performance. In the Energy area, this involves the use of new materials to transform and to store energy, including novel wearable and implantable energy systems for the use in medical technologies. In order to facilitate the creation of functional devices from fundamental discoveries he has pioneered the development of 3D additive fabrication (including 3D printing) using advanced materials. He is committed to fundamental research and the translation of fundamental discoveries into practical applications. He is a passionate communicator, dedicated to explaining scientific advances to all in the community from the layperson to the specialist.

Scientific Advisory Board The Scientific Advisory Board (SAB) has an independent mentoring and advisory role to the Executive, providing strategic insight and commercial direction to the Centre. The SAB plays a key role in advising the Centre on the development of the strategic and commercialisation plans. The Board advises on patent versus publication strategy, and commercialisation and spin-out strategy as required. They assist in identifying intellectual property, partnering and licensing opportunities and organisations with which CBNS can ally. In addition to the core members of the SAB, there are temporary international members, including the CBNS Visiting Professor, who provide variation to the scientific mix of the SAB. Professor Alan Rowan

Professor Rowan has performed his research at the interface of chemistry and biology with seminal and pioneering work on processive catalysis and functional self-assembly. His latest scientific achievement has been the development of the first truly biomimetic hydrogel which mimics the mechanic and functional properties of the extracellular membrane.

from magnetic materials, single enzyme catalysis, supramolecular catalysis through to nanometresized solar cells and photonic materials. In the last ten years, he developed the concept of processive catalysis, mimicking the natural exo- and endonucleases and demonstrated that a macrocyclic catalyst can thread onto and move along a polymer substrate in a highly efficient process.

This scientific breakthrough is now being developed commercially for wound dressing, drug therapeutic and cell growth. This has further established Professor Rowan as a truly innovative scientist, working toward understanding at the molecular level the functionality of hierarchical materials and catalysis.

In January 2016 Professor Rowan joined the University of Queensland as the Director of the Australian Institute of Bioengineering and Nanotechnology. He previously led a research group at Radboud University’s Institute of Molecules and Materials, one of Europe’s leading research centres for the nanoscience’s. He was awarded an ARC Australian Laureate Fellowship to continue his work on novel biomimetic and dynamic materials.

Professor Rowan’s considerable scientific curiosities has resulted in him working in many areas, ranging

Dr David Owen

Dr Owen has driven the drug delivery programs by developing and executing a number of successful proof-of-concept studies. The results from these studies have led to a number of commercial partnerships such as Stiefel, a GSK company, Lilly and AstraZeneca, as well as driving Starpharma’s own internal drug delivery program focused on an improved dendrimer-docetaxel formulation.

Dr Julio Ribeiro

Dr Ribeiro completed his PhD in Medicine at UNSW in 1998. He then moved from academia to start a business carried at Sigma-Aldrich Pty. Ltd., Australia and NZ, where he had many executive roles, including technical support manager, sales manager and manager of a fine chemical division (SAFC) in Australia and NZ. In 2006, Dr Ribeiro founded Inventia Pty Ltd, a fine chemical and biotechnology company. As Managing Director, Dr Ribeiro oversees R&D projects from conceptualisation to delivery. This includes the conception of projects, contracting scientists, daily operations and writing final reports based

on scientists’ results. In its short period of existence, Inventia has landed several R&D contracts. In 2012, Dr Ribeiro created Inventia Genetic Technologies Pty Ltd (IGT). A subsidiary of Inventia Pty Ltd. IGT is now Australian leading provider of bovine IVF for the beef farmers in QLD. IGT has been conducting several research projects in the field of bovine IVF with the objective of increasing the pregnancy rate; facilitate oocytes and embryo transport and development of new bovine IVF media. In 2012, in partnership with UNSW, Dr Ribeiro started the 3D Bioprinting project; commercialising the 3D Bioprinting technology. This resulted in the creation of Inventia Life Science Pty Ltd in 2013.

CBNS Annual Report 2019 59

G OVE R NAN C E

Dr Owen is the Vice President of Research at Starpharma and has extensive experience in medicinal chemistry and biochemistry, and in managing teams focused on commercially directed drug discovery. He has held several positions in the biotech industry including Mimotopes, Cerylid and Glykoz and has gathered extensive international experience in biotechnology and pharmaceutical research and development. Since joining Starpharma

Professor Jagat R. Kanwar – until November 2019

Professor Kanwar is the Head and team leader of Nanomedicine-Laboratory of Immunology and Molecular Biomedical Research (NLIMBR), School of Medicine, Faculty of Health, Deakin University, Australia. Professor Kanwar has an international reputation and expertise in investigating fundamental and applied molecular signalling aspects of the pathogenesis of cancer, chronic inflammation and neurodegenerative diseases, thereby, leading to the development of treatment strategies from bench to bedside. He has more than 150 research publications in high impact factor and peer-reviewed international journals, 27 book chapters and 3 edited books. Professor Kanwar’s research has generated several patents/PCTs with more than five licensed patents for commercialization to Biopharma industry. His research combines Immunology with state of the art and cutting-edge techniques in Molecular

Biology, Biochemistry, Nano-biotechnology and visualization to investigate the pathways in which key molecules are regulated in both normal and disease states. A number of in vitro human cell/tissue-based co-culture models for cancers, microbial infections; autoimmune diseases; chronic inflammatory diseases (osteoarthritis, inflammatory bowel disease), gut health, neurodegeneration and immunomodulation have been developed by his group. Kanwar’s main research objective is to understand and target the mechanisms involved at the molecular and subcellular level which gives us an edge over the prevalent targeting techniques. He carries out both academic and commercial research projects and develops new approaches for the diagnosis, treatment, and nanomedicine based new generation delivery systems.

Professor Fariba Dehghani

Professor Dehghani is the Director of ARC Food Processing Training Centre and the Director of Centre for Excellence for Advanced Food Enginomics at the University of Sydney, an Associate member of IChemE and a member of ARC College of Experts. She has been selected by Engineers Australia as one of the top 50 Engineers for innovation in research in 2016. She is a reputed researcher who has been instrumental in building an interdisciplinary research environment that stimulates the interface and intersects of engineering, science, business, medicine, pharmacy, and agriculture. Her international team of researchers and industry partners have worked together to provide pragmatic, cost-effective and environmentally sustainable solutions to a diverse range of issues, with the aim of improving lives. Professor Dehghani’s research has made significant contributions to the design of advanced and environmentally friendly techniques for processing

materials; the extraction of high value and active compounds from natural resources for the treatment and prevention of chronic diseases such as infection and cardiovascular disease; and the synthesis of materials for musculoskeletal tissue repairs. Her research is highly regarded, not only in academia but by industry. She has established long-term collaborations with the food and biotech private sectors and acquired substantive funding from national and international competitive grant applications. She has published more than 250 papers and is co-inventor on 13 patents, including nine with potential end users. She has mentored more than 45 postgraduates, more than 100 honours/masters researchers and several successful entrepreneurs. Her research led to the establishment of a start-up Company, Trimph Pty Ltd, in 2015 by receiving more than $2 M fund from a private investor and from government. The product from this company has recently received approval from TGA for the clinical application.

Professor Magdalena Plebanski – from November 2019

Professor Plebanski has a PhD (Immunology), MBA (Business), DPS (Psychology), BScHon (Biomedicine). She is also an NHMRC Senior Research Fellow. She is a Director of the Biomedical and Health Innovation Enabling Capability Platform (BHI-ECP) at RMIT University, as well as Head of the Translational Immunology and Nanotechnology Program at School of Health and Biomedical Sciences at RMIT. An interdisciplinary research leader in nanotechnology for biomedical innovation, leveraging collaborations with engineers and clinicians to develop new approaches to promote human health. Her primary interest is to develop practical immune therapies and vaccines against complex diseases, specifically cancer, malaria and asthma, pioneering the use of synthetic size-defined non-inflammatory nanoparticles. Professor Plebanski has over 175 peer-reviewed publications plus multiple published book chapters and abstracts, including field changing findings on vaccines and on immune evasion mechanisms used by parasites

60 CBNS Annual Report 2019

and cancer cells including top world-class journals: Science, Nature, Nature Biotechnology, Immunity, Nature Medicine, PLOS Pathogens, PNAS, Nature Communications and Lancet. Currently studying immunity in multiple human trials: of vaccines in the elderly or to validate new diagnostics, prognostics and treatments for ovarian cancer, together with Charitable Organisations, Universities, Hospitals and large Pharma. Approximately 50 patents in 10 patent families. Successfully progressed findings into human trials or commercialisation in diverse roles as an inventor, CSO, CEO and Director in biotechnology companies nationally and internationally. Current interests further involve big data analysis for the optimised application of vaccines and chemotherapy (personalised medicine or precision medicine) to vulnerable populations such as the elderly, as well as to patients with cancer, as well as the promotion of holistic, integrated, compassionate approaches to human health.

2019 temporary members Professor Chunying Chen

Professor Chen received her Bachelor’s degree in chemistry (1991) and obtained her PhD degree in Biomedical engineering (1996) from Huazhong University of Science and Technology of China. She worked as a postdoctoral research fellow at the Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics of Chinese Academy of Sciences (1996-1998) and at the Medical Nobel Institute for Biochemistry of Karolinska Institute, Sweden (2001-2002). From 2002 onwards, she

has been working as a group and project leader at the China Nanosafety lab. Professor Chen has been awarded the National Award for Innovation and Outstanding Service to the Standard authorised by Standardization Administration of the People’s Republic of China in 2011, the Second Prize of Beijing Science and Technology (ranked second) in 2008, the Second Prize of the National Natural Science Award (ranked second) in 2012.

Professor Irene Yarovsky

Professor Yarovsky is a Professor of Theoretical Physics and Program Leader for Biophysics and Bioengineering of the Health Innovations Research Institute of RMIT University, Melbourne, Australia. She completed her PhD in Monash University in 1994 on the topic of theoretical computer simulations of protein interactions with functionalised surfaces, under supervision of Professor Milton Hearn. She then joined BHP Research Laboratory in Melbourne where during the next 6 years she applied computational modelling to design advanced industrial materials and processes. At present, she is particularly interested in studying the response of biological systems to environmental triggers, including

electromagnetic fields. Of particular relevance to ACEBR is Irene’s expertise in development and application of innovative computer simulation strategies to study the external stress response of biomolecules in different environments at the atomic level. In her projects supported by the ARC Linkage scheme she employed atomistic simulations to study the effects of non-ionising radiation on protein structure and dynamics, including their ability to form fibrillar aggregates. This work has shed light on the response of insulin to static and oscillating electric fields and laid a foundation for further research on biomolecular interactions with electromagnetic radiation proposed in ACEBR.

Professor Joseph Wang

publications have been cited approximately over 101,000 times and his H-index is 157. He received several national ACS and ECS Awards in Instrumentation and Electrochemistry, 10 Honorary Professors and 3 Medals of honors from different countries. Professor Wang is a Fellow of Royal Society of Chemistry (RSC) and of the American Institute of Medical and Biological Engineering (AIMBE). He has guided over 250 PhD students and post-doc fellows over his career

Professor Ranjeny Thomas

Professor Thomas is a Professor of Rheumatology at University of Queensland, Translational Research Institute, consultant rheumatologist at Princess Alexandra Hospital and fellow of the Australian Academy of Health and Medical Sciences. Her research seeks to understand autoimmune disease and restoration of immune tolerance. Through this work, she developed and tested the

first rheumatoid arthritis vaccine. She has also contributed major insights into how the microbiome is involved in causing spondyloarthropathy leading to the development of disease biomarkers and therapeutic strategies. Ranjeny is founder and a director of the spin-off company, Dendright, which is developing immunotherapy for autoimmune diseases.

CBNS Annual Report 2019 61

G OVE R NAN C E

Professor Wang is a Distinguished Professor, SAIC Endowed Chair and Chair in Department of Nanoengineering at University of California, San Diego (UCSD). He is also the Director of the UCSD Center of Wearable Sensors and Chief Editor of Electroanalysis. He served as the director of Center for Bioelectronics of ASU before joining UCSD. Wang has made pioneering contributions to wearable biosensors, nanomachines and nanobioelectronics. Prof. Wang has published more than 1060 papers; 11 books and he holds 30 patents. These

62 CBNS Annual Report 2019

CBNS Annual Report 2019 63

P E R FO R MAN C E

Performance

Performance and KPIs CBNS achievements are evaluated by the Australian Research Council on an annual basis. CBNS performance is assessed against key performance indicators (KPIs) that were set at the commencement of the Centre and have been reviewed in 2017. These cover the traditional research metrics of outputs like journal publications and conference presentations. There are also metrics that cover the interdisciplinarity of CBNS research, new research partnerships, measures of esteem, public awareness and student and ECR mentoring. The KPIs for 2019 are summarised in this infographic; it shows the targets for each of the KPI areas as well as the Centreâ&#x20AC;&#x2122;s actual achievements.

RESEARCH FINDINGS: Research outputs: Journal publications target: 220 actual: 226

Patents (filed) target: 3 actual: 14

Book chapters target: 5 actual: 4

Quality of research outputs: Publications impact factor >10 target: 18

actual: 49

% journals articles impact factor >2 target: 70%

New research fellowships target: 3

Membership on editorial boards target: 25

actual: 91%

actual: 10

actual: 71

RESEARCH TRAINING AND PROFESSIONAL EDUCATION: Training courses Workshops/conferences held/offered by the Centre: Professional training courses for staff and postgraduate students attended target: 15

Centre attendees at all training/development courses offered by the Centre target: 75

actual: 25

actual: 166

National workshops held/organised by the Centre target: 5

International workshops held/organised by the Centre target: 1

actual: 7

actual: 2 Postgraduate completions:

Additional researchers working on Centre research: New postgraduate students working on core Centre research and supervised by Centre staff target: 8

New postdoctoral researchers recruited to CBNS working on core CBNS research target: 4

New Honours students working on core CBNS research and supervised by Centre staff target: 12

actual: 35

actual: 19

actual: 10

ECRs (within five years of completing PhD) working on core CBNS research target: 20

actual: 76

Completions by PhD students target: 15 actual: 19 Completions by Masters students target: 2 actual: 3 Completions by Honours students target: 8 actual: 8

PRESENTATIONS/BRIEFINGS: Briefings given to government, industry and business community target: 10

actual: 16

64 CBNS Annual Report 2019

Public awareness/outreach programs (include outreach activities such as public lectures, public events at the national nanomedicine conference, participation in National Science Week and vodcasts) target: 10 actual: 14

Talks given by Centre staff open to the public target: 10

actual: 16

New organisations collaborating with, or involved in, the Centre target: 15 actual: 65

Mentoring programs offered by the Centre target: 7 actual: 11 Students mentored, target: 80 actual: 194

GENDER EQUITY AND DIVERSITY:

Centre-led initiatives that promote gender equity and diversity and a family-friendly workplace target: 4

% meetings and workshops facilitated by the Centre that enable attendance by members with dependents target: 70%

% Centre-organised meetings with gender balance amongst organising committees, speakers and participants target: 70%

actual: 15

actual: 83%

actual: 71%

% of women in mentoring program target: 50%

ECRS and students holding leadership roles within the Centre target: 30

actual: 60%

actual: 53

COLLABORATION AND INTERDISCIPLINARITY:

Research outputs featuring co-authorship between nodes target: 20

actual: 40

Research outputs that result from Centre interdisciplinary research target: 12

Postgrads or postdocs working on cross node or interdisciplinary projects target: 12

actual: 141

actual: 180

Postgrads or postdocs that have moved (i.e. employment) between collaborating organisations and partner organisations to work on research projects, target: 5

Interdisciplinary projects in the research program target: 14

actual: 81

actual: 6

Visits to overseas laboratories and facilities target: 30

actual: 35

International visitors and visiting fellows target: 40

actual: 46

Invited talks/papers/ keynote lectures given at major international meetings target: 60

Commentaries about the Centreâ&#x20AC;&#x2122;s achievements

actual: 101

Articles target: 40

Media releases target: 15

actual: 109

actual: 16

Research outputs co-authored with alumni target: 8

actual: 25

Alumni participating in Centre research program (e.g. mentor, invited speaker, student supervisor, lab visit-exchange, project collaborator), target: 8

Website hits (page views) target: 40,000

actual: 51,462

Video material produced and publicised each year on the website (mins) target: >20mins

actual: 59mins

actual: 24

CBNS Annual Report 2019 65

P E R FO R MAN C E

PROFILE AND SCIENTIFIC LEADERSHIP:

Financial Report 2019 INCOME AND EXPENDITURE Income

IN KIND CONTRIBUTIONS 2019 ($)

Institution

2019 ($)

ARC Grant Income

4,107,842

Monash University

1,476,593

Collaborating organisation contribution

1,608,048

University of Melbourne

1,328,854

Other income (including interest distribution)

10,680

Total income

5,726,570

Expenditure

2019 ($)

University of New South Wales

440,000

University of Queensland

636,468

University of South Australia

1,304,805

Salaries 4,368,229

Australian Synchrotron

Equipment 101,414

Australian Nuclear Science and Technology Organisation 69,500

Consumables and maintenance

764,831

Travela# 377,483 Scholarships and Student support

395,309

Administration 67,486 Otherb# 288,574 Total expenditure

Carry forward

6,363,325

2,263,310

Sungkyunkwan University

644,752

University of Wisconsin-Madison

15,000

University of Warwick

66,000

University of Nottingham

48,715

Imperial College London

5,000

Memorial Sloan-Kettering Cancer Center University College Dublin

60,000 5,000

Carry forward adjustmentc 398

University of California, Santa Barbara

26,000

Balance 1,626,555

Columbia University Medical Center

69,810

a Includes accommodation, workshop and conference expenses b Includes finance-related costs, telecommunication, hospitality c Adjustment made in annual report for 2019 carry forward to recover costs under Other Expenditure # Includes expenses for education, outreach and industry programs

CASH CONTRIBUTIONS Institution

2019 ($)

Monash University

375,729

University of Melbourne

335,318

University of New South Wales

421,503

University of Queensland

292,169

University of South Australia

183,328

Total 1,608,048

66 CBNS Annual Report 2019

Total 6,196,497

Awards, Memberships and Grant Success

Member of the Order of Australia & Lemberg Medal CBNS CI Professor Maria Kavallaris AM (UNSW) was appointed a Member of the Order of Australia. She was included in the 2019 Australia Day Honours List for her significant service to medicine, and to medical research, in the field of childhood and adult cancers. Furthermore, Professor Maria Kavallaris AM was selected to receive the prestigious Lemberg Medal by the Australian Society for Biochemistry and Molecular Biology. Awarded annually, the Lemberg Medal is presented in memory of Emeritus Professor M.R. Lemberg, who was the Society’s first President and Honorary Member. Leverhulme Medal CBNS Deputy Director Professor Frank Caruso (UoM) was awarded The Leverhulme Medal, from The Royal Society, for driving the application of engineered particles in biology and medicine through nanoscale materials engineering. The Royal Society is a Fellowship of many of the world’s most eminent scientists and is the oldest scientific academy in continuous existence. The Leverhulme Medal is awarded for an outstanding researcher in the field of chemical engineering and applied chemistry every 3 years. ARC Future Fellowship Dr Kristian Kempe (Monash) was awarded an ARC Future Fellowship for his project Expanding the toolbox of synthetic stealth polymers. His project aims to develop a new generation of synthetic and biomimetic pseudo peptide polymers with advanced biomedical properties. The polymers will be used for the fabrication of a range of nanoparticles, which will allow to study the effect of architecture, composition and surface functionalisation on their behaviour in a biological environment.

ARC Linkage Project Dr Craig Bell, Dr Chris Howard and CI Professor Kris Thurecht (UQ) were awarded an ARC Linkage Grant for their project Smart affinity membranes for manufacture of high-value therapeutic proteins. This project aims to develop next-generation separation membranes for production of high-value proteins from serum.

Dr Winter and her team, including CBNS Node Leader and CI Professor Benjamin Thierry will investigate the use of tiny drug carriers to treat – and even prevent – pre-eclampsia and its complications without affecting the fetus. A focus of the 18-month project is to develop potential treatments compatible with low resource and/or remote settings, including regional Australia and lowincome countries which often suffer the most severe consequences of the disease.

Awards

Homeward Bound Homeward Bound (HB) is a global leadership initiative, set against the backdrop of Antarctica, which aims to heighten the influence and impact of women in making decisions that shape our planet. Ms Aria Ahmed-Cox (UNSW) was awarded a place within the 5th cohort of future leaders. Over 290 women, from 35 countries applied. Like the four cohorts that have gone before them, The Team enter their leadership journey via an 11-month online curriculum covering leadership development, strategic capability, visibility and science. In November 2020, they will all meet face-to-face for the first time in Ushuaia, Argentina, before embarking on their three week voyage to Antarctica.

Professor Frank Caruso (UoM) Leverhulme Award, The Royal Society

Grand Challenges Explorations Dr Marnie Winter (UniSA) has been awarded US$100,000 (AUD 157,000) from the world’s largest private foundation to help better understand and tackle a condition which kills 76,000 women and 500,000 babies each year; pre-eclampsia.

Dr Simona Carbone (Monash) American Journal of PhysiologyGastrointestinal and Liver Physiology

The international team has received funding from the Grand Challenges Explorations, an initiative of the Bill & Melinda Gates Foundation, to explore cutting-edge nanotechnology and bioengineering approaches to address pre-eclampsia, one of the most common complications in pregnancy.

Professor Maria Kavallaris AM (UNSW) Member of the Order of Australia Lemberg Medal, Australian Society for Biochemistry and Molecular Biology UTS Chancellor’s Award for Excellence UTS Science Award

Member of Editorial Boards Professor Ben Boyd (Monash) Current Drug Delivery Drug Delivery and Translational Research Journal of Colloid and Interface Science Journal of Liposome Research Journal of Pharmaceutical Sciences Journal of Pharmacy and Pharmacology

Professor Frank Caruso (UoM) ACS Applied Bio Materials ACS Central Science ACS Nano Advanced Functional Materials Advanced Healthcare Materials Advanced Science Angewandte Chemie Biomacromolecules Biomaterials Science CCS Chemistry Chemistry of Materials Nano Today ACS Materials Letters

CBNS Annual Report 2019 67

P E R FO R MAN C E

Highlights

Professor Edmund Crampin (UoM) Bulletin of Mathematical Biology Current Opinion in Systems Biology npj Systems Biology and Applications Professor Tom Davis (Monash) Polymer Science ACS Macro letters Professor Justin Gooding (UNSW) Editor-in-Chief, ACS Sensors American Scientific Publishers Journal of Chemical and Biological Interfaces Elsevier journal Electrochemistry Communications Wiley journal, Electroanalysis Elsevier journal Sensors and Actuators B MDPI journal Biosensors Wiley-VCH journal, ChemElectroChem ACS journal, Bioconjugate Chemistry Wiley-VCH journal, Chemistry – A European Journal MDPI journal, Nanomaterials Professor Maria Kavallaris AM (UNSW) British Journal of Pharmacology American Journal of Cancer Research Open access Proteome Journal Associate Professor Matthew Kearnes (UNSW) Science as Culture Science Technology and Society Professor Stephen Kent (UoM) Frontiers in Immunology Journal of Virology Sexual Health The Journal of Immunolgy Vaccine npj Vaccines Immunology & Cell Biology Clinical & Translational Immunology Professor Rob Parton (UoM) Editor in Chief, Traffic Associate Editor, Molecular Biology of the Cell International Editorial Board – Cell Structure and Function Dr Daniel Poole (Monash) American Journal of PhysiologyGastrointestinal and Liver Physiology Professor Chris Porter (Monash) Molecular Pharmaceutics Journal of Pharmaceutical Sciences Pharmaceutical Research Journal of Pharmacy and Pharmacology

68 CBNS Annual Report 2019

Professor Clive Prestidge (UniSA) Pharmaceutics Journal of Liposome Research Open Material Science AAPS Open Professor Benjamin Thierry (UniSA) Plos One Scientific Reports Professor Pall Thordarson (UNSW) Australian Journal of Chemistry ChemSystemsChem Cell Reports Physical Science Professor Kris Thurecht (UQ) Associate Editor, Molecular Pharmaceutics Dr Nik Veldhuis (Monash) American Journal of PhysiologyGastrointestinal and Liver Physiology Professor Andrew Whittaker (UQ) Chinese Journal of Polymer Science Radiation Physics and Chemistry Journal of Applied Polymer Science Dr Cheng Zhang (UQ) Journal of Nanoscience and Nanomedicine

Committee and Other Memberships Professor Maria Kavallaris AM (UNSW) Chair, 10th International Nanomedicine Conference 24-26 June 2019 Scientific Advisory Board Member, TroBio Therapeutics NHMRC Research Committee Member ARC National Competitive Grants Program (NCGP) Assessor, 2019-2020 NHMRC Research Committee Member (appointed by Federal Minister for Health) Fellow, Australian Academy of Health and Medical Sciences (AAHMS) Chair, Australian Institute of Policy & Science Chair, Executive Committee, Cancer Cytoskeleton Network, OSMR, NSW Government Member of the Board – Australian Institute for Policy and Science Cure Cancer Australia Foundation Ambassador Associate Professor John McGhee (UNSW) Head of Program & Logistics for the SIGGRAPH 2019 Conference

Grant Success During 2019, additional funding was secured to further support research being undertaken at the Centre. This funding reinforces the collaborative nature and expertise of CBNS researchers. Details of grant successes are as follows:

ARC grants The following CBNS researchers secured additional funding through ARC grants: Discovery Outcome, Linkage, Infrastructure Equipment and Facilities (LIEF) Outcome and Linkage Project: Dr Craig Bell (UQ) Professor Frank Caruso (UoM) Professor Tom Davis (Monash) Dr Thomas Hall (UQ) Dr Chris Howard (UQ) Dr Angus Johnston (Monash) Associate Professor Matthew Kearnes (UNSW) Professor Stephen Kent (UoM) Professor Robert Parton (UQ) Dr Nghia Truong Phuoc (Monash) Dr Daniel Poole (Monash) Professor Chris Porter (Monash) Dr John Quinn (Monash) Professor Pall Thordarson (UNSW) Professor Kris Thurecht (UQ) Dr Nik Veldhuis (Monash) Dr Mikey Whittaker (Monash)

National Health and Medical Research Council (NHMRC) Grants The following CBNS researchers secured additional funding through National Health Research Medical Research Council (NHRMC) grants: Development, Project and Funding Outcome: Professor Benjamin Thierry (UniSA) Professor Chris Porter (Monash)

Other grants Professor Frank Caruso (UoM) Dr Christina Cortez-Jugo (UoM) Professor Justin Gooding (UNSW) Dr Kristian Kempe (Monash) Mr Priyank Shenoy (Monash) Dr Marnie Winter (UniSA)

NHMRC Fellowships Dr Adam Wheatley (UoM) NHMRC Emerging Leadership Fellowship

Dr Kristian Kempe (Monash) ARC Future Fellowship Dr Stuart Johnston (UoM) ARC Discovery Early Career Researcher Award Dr Simona Carbone (Monash) ARC Discovery Early Career Researcher Award

Other Fellowships Dr Alessia Amodio (UoM) Marie Skłodowska-Curie Actions (MSCA) Research Fellowship Mr Denzil Furtado (UoM) Main Sequence Ventures Nova Residency Dr Stuart Johnston (UoM) Victoria Fellowship

at the International Conference on Nanoscience and Nanotechnology (ICONN2020)

ANN Conference Travel Bursary – a travel bursary to attend the 10th International Nanomedicine Conference

Ms Caterina Brighi (UQ) 2019 COGNO Young Investigator Award at the 12th COGNO Annual Scientific Meeting

Ms Santhni Subramaniam (UniSA) ACS Applied Nanomaterials 3MT Poster Presentation Prize at the 10th International Nanomedicine Conference, Sydney ANN Conference Travel Bursary – a travel bursary to attend the 10th International Nanomedicine Conference Patrick Couvreur Student Travel Award at the 46th Controlled Release Society Annual Meeting & Exposition, Valencia CRS Young Scientist Travel Grant at the 46th Annual Meeting & Exposition, Valencia

Mrs Ava Faridi (Monash) Best oral presentation at the ICNNN 2019: International Conference on Nanobiotechnology, Nanobiology and Nanomedicine, Montreal, Canada Dr Christian Fercher (UQ) FBNS 2019 Best Poster Ms Sharmin Hoque (UNSW) Honourable mention to appreciate the quality talk at the ISE student symposium on electrochemistry

Dr René Lafleur (UoM) NWO Rubicon postdoctoral fellowship

Dr Yi Ju (UoM) Nanoscale Advances Oral Prize Early Career Researcher Grant Scheme (UoM)

Dr Meike Leiske (Monash) Feodor-Lynen Postdoc Fellowship from the Alexander Von Humboldt Foundation (Germany)

Mr John Kokkinos (UNSW) Winner UNSW 3-Minute Thesis Competition Tour de Cure PhD Support Scholarship

Mr Yutian Ma (UoM) Harold Mitchell Postgraduate Travelling Fellowship

Ms Zerong (Shirley) Ma (UNSW) Best Oral Presentation Award at the 10th International Nanomedicine Conference

Associate Professor John McGhee (UNSW) UNSW Scientia Fellowship Program Dr Angel Tan (Monash) Endeavour Research Leadership Fellowship (Global Leadership) Dr Yunxin (Cindy) Xiao (Monash) Australian Nanotechnology Network (ANN) Overseas Travel Fellowship (OTP)

Postdoctorate and Student Awards Dr John Bailey (UNSW) UNSW Scientia PhD Scholarship Ms Aria Ahmed-Cox (UNSW) Homeward Bound EACR Travel Bursary at the EACR Nanotechnology in Cancer: Engineering for Oncology Dr Niloufar Ansari (Monash) ANZBMS Travel Grant at the Australian and New Zealand Bone and Mineral Society (ANZBMS) Annual Meeting Mr Stefan Bader (Monash) The Freemasons Victoria Lodge of Australia Felix Cancer Research Grant

Ms Sajedeh Maghrebi (UniSA) 2019 Patrick Couvreur Student Travel Award from the Controlled Released Society (CRS) Ms Ayaat Mahmoud (Monash) People’s Choice Award for Poster Presentation at the Monash 14th Annual Graduate Research Symposium Dr Friederike Mansfield (UNSW) Australian Centre for Nanomedicine Travel Award Dr Angelica Merlot (UNSW) NSW Young Woman of the Year Mr Quang Nghia Pham (UniSA) Healthy Development Adelaide and Channel 7 Children’s Research Foundation – PhD Excellence Award Dr JJ Richardson (UoM) FBNS 2019 - Spark Tank Ms Hayley Schultz (UniSA) BPW Adelaide Heather Southcott Memorial Grant – travel bursary to attend the AAPS PharmSci360 Conference in Texas

Ms Xiaohan Sun (Monash) CRS 2019 Local Chapter Young Scientist Travel Grant at the 2019 CRS Annual Meeting and Exposition CRS Mind Mapping Championship Ms Chelsea Thorn (UniSA) Endeavour Postgraduate Leadership Dr Kristel Chayadi Tjandra (UNSW) FBNS 2019 - Best Oral Session Dr Marnie Winter (UniSA) South Australian Young Tall Poppy of Science Thrasher Early Career Award Ms Leah Wright (UniSA) Endeavour Postgraduate Leadership Dr Yanfang Wu (UNSW) Dean’s Award for Outstanding PhD Thesis ACN Travel Award Dr Yunxin (Cindy) Xiao (Monash) Australian Institute of Nuclear Science and Engineering (AINSE) International Travel Scholarship at the International Polymer Colloids Group Conference (IPCG), Singapore 1st Oral Presentation Prize at the Australian Centre for Neutron Scattering (ACNS) Neutron Clip Day, ANSTO, Sydney Best Oral Presentation at the Frontiers in Bio-Nano Science Symposium (FBNS) 2nd Oral Presentation Prize at the Australian X-ray Analytical Association (AXAA) Victoria Student Seminar Best Poster Award at the Drug Delivery Australia (DDA) Conference 2019 Mr Qizhi Zhong (UoM) Eugen Singer Award

CBNS Annual Report 2019 69

P E R FO R MAN C E

ARC Fellowships

CBNS Personnel Chief Investigators Professor Tom Davis ARC Laureate Fellow, CBNS Director, Node Leader, Imaging and Visualisation Monash University

Professor Frank Caruso ARC Laureate Fellow, CBNS Deputy Director, Node Leader, Delivery Systems and Vaccines University of Melbourne

Professor Justin Gooding ARC Laureate Fellow, Node Leader, Sensors and Diagnostics Theme Leader, Delivery Systems and Vaccines University of New South Wales

Professor Andrew Whittaker Node Leader, Imaging and Visualisation Theme Leader University of Queensland

Professor Christopher Porter Delivery Systems and Vaccines Theme Leader Monash University

Professor Stephen Kent Delivery Systems and Vaccines Theme Leader University of Melbourne

70 CBNS Annual Report 2019

Professor Benjamin Thierry Node Leader, Sensors and Diagnostics University of South Australia

Professor Ben Boyd Delivery Systems and Vaccines Monash University

Dr Angus Johnston Delivery Systems and Vaccines, Imaging and Visualisation Monash University

Dr Simon Corrie Sensors and Diagnostics University of Queensland

Professor Edmund Crampin Delivery Systems and Vaccines University of Melbourne

Professor Maria Kavallaris AM Imaging and Visualisation, Sensors and Diagnostics, Delivery Systems and Vaccines University of New South Wales

Professor Pall Thordarson Delivery Systems and Vaccines, Sensors and Diagnostics, Strategic Project University of New South Wales

Associate Professor Matthew Kearnes Imaging and Visualisation University of New South Wales

Associate Professor John McGhee Imaging and Visualisation University of New South Wales

Professor Kris Thurecht Delivery Systems and Vaccines, Imaging and Visualisation, Sensors and Diagnostics University of Queensland

Professor Rob Parton Delivery Systems and Vaccines, Imaging and Visualisation University of Queensland

Professor Clive Prestidge Delivery Systems and Vaccines University of South Australia

Partner Investigators

Professor Cameron Alexander Head of Division of Drug Delivery and Tissue Engineering, Faculty of Science University of Nottingham, UK Professor Nigel Bunnett Chair, Department of Basic Science and Craniofacial Biology Columbia University Medical Center, USA

Professor Kenneth Dawson Director of the Centre for BioNano Interactions, Chair of Physical Chemistry University College Dublin, Ireland

Professor Doo Sung Lee Director Theranostic Macromolecules Research Center, Dean of College of Engineering Sungkyunkwan University, South Korea

Dr Ivan Greguric Head of Radiochemistry Australian Nuclear Science and Technology Organisation, Australia

Professor Jason Lewis Vice Chair for Research, Chief of the Radiochemistry and Imaging Sciences Service Memorial Sloan Kettering Cancer Center, USA

Professor David Haddleton Head of Inorganic and Materials Section, Department of Chemistry University of Warwick, UK

Professor Craig Hawker Director of the California Nanosystems Institute, Dow Materials Institute, Co-Director of the Materials Research Lab University of California, Santa Barbara, USA

Professor Molly Stevens Research Director for Biomedical Material Sciences, Institute of Biomedical Engineering Imperial College London, UK

P E R FO R MAN C E

Professor Nicholas Abbott Tisch University Professor at Smith School of Chemical and Biomolecular Engineering University of Wisconsin-Madison, USA

CBNS Annual Report 2019 71

Research staff Postdoctoral and other research staff Dr Michele Bastiani Senior Research Fellow University of Queensland Dr Thomas Hall Senior Research Fellow University of Queensland Dr Pu-Chun Ke Senior Research Fellow Monash University Dr Kristian Kempe Senior Research Fellow Monash University Dr Alessia Amodio Postdoctoral Researcher University of Melbourne Dr Niloufar Ansari Postdoctoral Researcher Monash University Dr Md. Arifur Rahim Postdoctoral Researcher University of New South Wales Dr Lakmali Atapattu Postdoctoral Researcher University of New South Wales Dr John Bailey Postdoctoral Researcher University of New South Wales Dr Craig Bell Postdoctoral Researcher University of Queensland Dr Simon Brayford Postdoctoral Researcher University of New South Wales Dr Kristel Cahyadi Tjandra Postdoctoral Researcher University of New South Wales Dr Enyuan Cao Postdoctoral Researcher Monash University Dr Simona Carbone Postdoctoral Researcher Monash University Dr Anna Cifuentes-Rius Postdoctoral Researcher Monash University Dr Christina Cortez-Jugo Postdoctoral Researcher University of Melbourne Dr Aidan Cousins Postdoctoral Researcher University of South Australia Dr Peter Cudmore Postdoctoral Researcher University of Melbourne

72 CBNS Annual Report 2019

Dr Robert De Rose Postdoctoral Researcher Monash University

Dr Christopher Howard Postdoctoral Researcher University of Queensland

Dr Andrew Lilja Postdoctoral Researcher University of New South Wales

Dr Nicole Dmochowska Postdoctoral Researcher University of South Australia

Dr Luojuan Hu Postdoctoral Researcher Monash University

Dr Ye-Wheen Lim Postdoctoral Researcher University of Queensland

Dr Zil e Huma Postdoctoral Researcher Monash University

Dr Pie Huda Postdoctoral Researcher University of Queensland

Dr Fabio Lisi Postdoctoral Researcher University of New South Wales

Dr Francesca Ercole Postdoctoral Researcher Monash University

Dr Rowan Hughes Postdoctoral Researcher University of New South Wales

Dr Iman Manavitehrani Postdoctoral Researcher University of New South Wales

Dr Matthew Faria Postdoctoral Researcher University of Melbourne

Dr Daniel Hurley Postdoctoral Researcher University of Melbourne

Dr Friederike Mansfeld Postdoctoral Researcher University of New South Wales

Dr Orlagh Feeney Postdoctoral Researcher Monash University

Dr Manasi Jambhrunkar Postdoctoral Researcher University of South Australia

Dr Michelle Maritz Postdoctoral Researcher University of South Australia

Dr Christian Fercher Postdoctoral Researcher University of Queensland

Dr Stuart Johnston Postdoctoral Researcher University of Melbourne

Dr Angelica Merlot Postdoctoral Researcher University of New South Wales

Dr John Finnegan Postdoctoral Researcher Monash University

Dr Paul Joyce Postdoctoral Researcher University of South Australia

Dr Ernesto Moles Postdoctoral Researcher University of New South Wales

Dr Laura Fitzgerald Postdoctoral Researcher Monash University

Dr Yi (David) Ju Postdoctoral Researcher University of Melbourne

Dr Muneer Musthakahmed Postdoctoral Researcher University of Queensland

Dr Nicholas Fletcher Postdoctoral Researcher University of Queensland

Dr MoonSun Jung Postdoctoral Researcher University of New South Wales

Dr Azadeh Nilghaz Postdoctoral Researcher University of South Australia

Dr Changkui Fu Postdoctoral Researcher University of Queensland

Dr Shereen Kadir Postdoctoral Researcher University of New South Wales

Dr Ka Fung (Leo) Noi Postdoctoral Researcher Monash University

Dr Vinicius Goncales Postdoctoral Researcher University of New South Wales

Dr Aleksandr Kakinen Postdoctoral Researcher Monash University

Dr Sandra Nurttila Postdoctoral Researcher University of New South Wales

Dr Gracia Gracia Postdoctoral Researcher Monash University

Dr Declan Kuch Postdoctoral Researcher University of New South Wales

Dr Shuaijun Pan Postdoctoral Researcher University of Melbourne

Dr Bin Guan Postdoctoral Researcher University of South Australia

Dr David Ladd Postdoctoral Researcher University of Melbourne

Dr Hui Peng Postdoctoral Researcher University of Queensland

Dr Hanna Gustafsson Postdoctoral Researcher University of South Australia

Dr Alistair Laos Postdoctoral Researcher University of New South Wales

Dr Duy Phu Tran Postdoctoral Researcher University of South Australia

Dr Felicity Han Postdoctoral Researcher University of Queensland

Dr Marion Le Grand Postdoctoral Researcher University of New South Wales

Dr Daniel Poole Postdoctoral Researcher Monash University

Dr Sifei Han Postdoctoral Researcher Monash University

Dr Meike Leiske Postdoctoral Researcher Monash University

Dr Ruirui Qiao Postdoctoral Researcher Monash University

Dr Linda Hong Postdoctoral Researcher Monash University

Dr Nathania Leong Postdoctoral Researcher Monash University

Dr Tim Quach Postdoctoral Researcher Monash University

Dr Zachary Houston Postdoctoral Researcher University of Queensland

Dr Yuhuan Li Postdoctoral Researcher Monash University

Dr Pradeep Rajasekhar Postdoctoral Researcher Monash University

Dr Paulina Ramírez García Postdoctoral Researcher Monash University

Dr Daniel Yuen Postdoctoral Researcher Monash University

Ms Anita Kra Research Assistant University of South Australia

Mr Charles Ferguson Immunoem Facility Manager University of Queensland

Dr Joseph J. (JJ) Richardson Postdoctoral Researcher University of Melbourne

Dr Cheng Zhang Postdoctoral Researcher University of Queensland

Ms Cong (Constance) Li Research Assistant University of Melbourne

Mr Nick Gunn Lead Unity 3D Artist University of New South Wales

Dr Malinda Salim Postdoctoral Researcher Monash University

Dr Jiacheng Zhao Postdoctoral Researcher University of Queensland

Mr Christopher Lumb Research Assistant Monash University

Dr Susan Nixon Lab Manager University of Queensland

Dr Garima Sharma Postdoctoral Researcher Monash University

Dr Yuanhui Zheng Postdoctoral Researcher University of New South Wales

Mr Nick Martel Research Assistant University of Queensland

Mr Steve Spoljaric Lab Manager University of Melbourne

Dr Priyank Shenoy Postdoctoral Researcher Monash University

Dr Jiajing Zhou Postdoctoral Researcher University of Melbourne

Mr Mub Murshed Research Assistant Monash University

Mr Campbell Strong Lead Technical Developer University of New South Wales

Dr Angel Tan Postdoctoral Researcher Monash University

Dr René Lafleur Postdoctoral Researcher University of Melbourne

Ms Aparna Nandakumar Research Assistant Monash University

Ms Joyce Tang Visiting Scholar Monash University

Dr Dewan Taslima Akhter Postdoctoral Researcher University of Queensland

Ms Kathleen Kimpton Senior Research Assistant University of New South Wales

Ms Georgia Porter Research Assistant University of New South Wales

Mr Mohammad Amen Intern University of South Australia

Dr Karin Taylor Postdoctoral Researcher University of Queensland

Ms Rose-Marie Olsson Senior Research Assistant University of New South Wales

Mr James Rae Research Assistant University of Queensland

Ms Pauline Bredy Intern Monash University

Dr Nghia Truong Phuoc Postdoctoral Researcher Monash University

Ms Sheilajen (Vinca) Alcantara Research Assistant University of Melbourne

Ms Danielle Senyschyn Research Assistant Monash University

Mr Farouk Chorfi Intern Monash University

Ms Tara Tigani Research Assistant Monash University

Mr Ryan Clayden-Zabik Intern University of Queensland

Mr Anthony Wignall Research Assistant University of South Australia

Ms Elisa Fine Intern Monash University

Mr James Wilmot Research Assistant University of New South Wales

Mr Ezra Rex Intern University of South Australia

Mr Weizhi Xu Research Assistant University of Queensland

Ms Joyce Tang Intern University of Queensland

Dr Wenqian Wang Postdoctoral Researcher University of New South Wales Dr Adam Wheatley Postdoctoral Researcher University of Melbourne Dr Alison White Postdoctoral Researcher University of Queensland Dr Marnie Winter Postdoctoral Researcher University of South Australia Dr Alice Wong Postdoctoral Researcher University of New South Wales Dr Yanfang Wu Postdoctoral Researcher University of New South Wales Dr Hyon Xhi Tan Postdoctoral Researcher University of Melbourne Dr Chih-Tsung Yang Postdoctoral Researcher University of South Australia Dr Ying Yang Postdoctoral Researcher University of New South Wales

Mrs Thakshila Amarasena Research Assistant University of Melbourne Ms Jameelah Arreola Research Assistant University of Queensland Ms Anant (Annie) Bajwa Research Assistant University of Melbourne Ms Arisbel Batista Gondin Research Assistant Monash University Ms Moore Chen Research Assistant Monash University

Mr Edward Cheah Laboratory Assistant: Bioengineering University of South Australia

Ms Varvara Efremova Research Assistant University of New South Wales Ms Robyn Esterbauer Research Assistant University of Melbourne Mr Denzil Furtado Research Assistant University of Melbourne Ms Steph Hodby Research Assistant University of South Australia

P E R FO R MAN C E

Dr Nicholas Veldhuis Postdoctoral Researcher Monash University

Ms Kairi Koppel Research Assistant Monash University

CBNS Annual Report 2019 73

Students PhD students Mr Mohammed Abdul Awal University of South Australia Mr Nawfel Abdullah University of Queensland Ms Salma Ahmed University of Queensland Ms Aria Ahmed-Cox University of New South Wales Ms Maria Ai Kristine Tolentino University of New South Wales Ms Nabila Akhtar Monash University Ms Yassamin Albayaty University of South Australia Mr Fayaz Ali Larik University of New South Wales Mr Mohammadreza Alizadeh Ghodsi University of South Australia Ms Fida’a Alshawawreh University of New South Wales Mr Karrar Al-Taief University of New South Wales Ms Tara Alvarez Monash University Mr Muhammed Awad University of South Australia Mr Stefan Bader Monash University Ms Parisa Badiee University of South Australia Mr Arunpandian Balaji University of Queensland Ms Danielle Bennett University of New South Wales Mr Daniel Bobo University of Queensland Mr Simone Bonaccorsi University of New South Wales Mr Luke Bredberg University of South Australia Ms Caterina Brighi University of Queensland Mr Daniel Brundel Monash University Ms Bruna Cambraia Garms University of Queensland Mr Mehmet Can Zeybek University of Melbourne Mr David Chang University of New South Wales

74 CBNS Annual Report 2019

Ms Yixin Chang University of Queensland

Mr Saikat Ghosh University of Queensland

Ms Given Lee Monash University

Ms Dongfei Chen University of New South Wales

Ms Agata Glab University of Melbourne

Mr Hong Seng (Nick) Lee University of Queensland

Ms Xueqian Chen University of New South Wales

Mr James Grace Monash University

Ms Shiyao Li University of Melbourne

Ms Jingqu Rachel Chen University of Melbourne

Mr Zhaobin Guo University of South Australia

Ms Tang Li Monash University

Ms Rinku Chhasatia University of South Australia

Ms Han Han University of New South Wales

Ms Jiaxin Lily Lian University of New South Wales

Ms Evon Chong Monash University

Ms Yiyuan Han University of Melbourne

Mr Gan Lin University of Melbourne

Ms Weijing Chu University of Queensland

Mr Edward Henderson Monash University

Mr Zhixing Lin University of Melbourne

Mr Ryan Clayden-Zabik University of Queensland

Ms Sharmin Hoque University of New South Wales

Ms Feifei Liu University of Queensland

Ms Ewa Czuba-Wojnilowicz University of Melbourne

Mr Douglas Howard University of South Australia

Mr Yi Liu University of Melbourne

Mr Nam Dao Monash University

Ms Yingjie Hu University of Melbourne

Ms Amy Logan University of New South Wales

Mr Abbas Darestani Farahani University of New South Wales

Mr Lei Huang University of Melbourne

Ms Zhiruo Luo University of Queensland

Mr Pouya Dehghankelishadi University of South Australia

Mr James Humphries University of Queensland

Mr Yutian Ma University of Melbourne

Ms Ludivine Delon University of South Australia

Mr Gabriel Huynh Monash University

Ms Zerong Shirley Ma University of New South Wales

Mr Jesse Di Cello Monash University

Ms Susan Ireland University of New South Wales

Ms Saji Maghrebi University of South Australia

Mr Eric Du University of New South Wales

Mr Jiaul Islam Monash University

Mr Thai Duong Luong University of Queensland

Ms Nilou Jamshidi University of New South Wales

Ms Ayaat Mahmoud Mohamed Monash University

Ms Gayathri Ediriweera University of Queensland

Mr Phillip Janowicz University of Queensland

Ms Mona Elsemary University of South Australia

Mr Ibrahim Javed Monash University

Ms Aurelia Elz University of South Australia

Mr Zhengyang Jia University of South Australia

Mr Gerald Er University of Queensland

Ms Hannah Kelly University of Melbourne

Mr Sanjun Fan University of New South Wales

Mr Ujjaval Kerketta University of New South Wales

Ms Ava Faridi Monash University

Ms Vidhishri Kesarwani Monash University

Ms Sachini Fonseka University of Queensland

Ms Ana Klisuric University of South Australia

Ms Chelsea Forest University of New South Wales

Ms Ruby Kochappan Monash University

Ms Helen Forgham University of New South Wales

Ms Giri Krishnan University of South Australia

Mr Shreedar Gautam University of New South Wales

Ms May Lai Monash University

Ms Anna Gemmell University of Queensland

Ms Alina Lam Monash University

Ms Zihnil Mazrad Monash University Mr Milad Mehdipour University of New South Wales Ms Tahlia Meola University of South Australia Ms Valentina Milanova University of South Australia Ms Parisa Moazzam University of New South Wales Mr Alexander Moersdorf Monash University Mr Quang Nghia Pham University of South Australia Ms Duyen Nguyen University of New South Wales Ms Vy Nguyen University of South Australia Mr Thanh Nhan Nguyen Monash University Mr Peter O’Mara University of New South Wales

Mr Pietro Pacchin Tomanin University of Melbourne

Ms Xiaohan Sun Monash University

Mr Qizhi Zhong University of Melbourne

Mr Wen Mow Monash University

Ms Roberta Pala University of New South Wales

Ms Panthipa Suwannakot University of New South Wales

Ms Xiaotong Zhou Monash University

Mr Jose Parra University of Queensland

Mr Raheleh Pardekhorram University of New South Wales

Ms Estelle Suys Monash University

Mr Minze Zhu University of Queensland

Ms Amber Prior University of Queensland

Mr Scott Peng Monash University

Ms Wenxian Tang University of New South Wales

Ms Aadarsh Zia Monash University

Mr Ronny Sanchez University of Queensland

Mr Thuy Pham University of South Australia

Ms Serena Teo Monash University

Masters students

Mr Matthew Sims University of New South Wales

Ms Emily Pilkington Monash University

Ms Chelsea Thorn University of South Australia

Ms Ranjana Piya University of New South Wales

Mr Matthew Urquhart Monash University

Mr David Pizzi Monash University and The University of Queensland

Mr Adil Usman University of Queensland

Mr Abdur (Adil) Rehman University of New South Wales Mr Joshua Rennick Monash University Mr Jeffri Retamal Santibanez Monash University Mr Meysam Rezaeishahmirzadi University of South Australia Mr Kye Robinson University of Melbourne Ms Yih Rue Ong Monash University Mr Md Saiful Alam University of New South Wales Ms Jennifer Schoning University of Queensland Ms Hayley Schultz University of South Australia Mr Joshua Simpson University of Queensland Mr Amal Sivaram University of Queensland Ms Vanessa Soh University of Queensland Ms Jiaying Song University of Melbourne Ms Merryn Strange University of Queensland Mr Ian Styles Monash University Ms Gayathri Subramaniam University of Queensland Ms Santhni Subramaniam University of South Australia

Ms Florida Voli University of New South Wales Ms Ngoc Mai Vu Monash University Mr Cong Vu Thanh University of New South Wales Ms Julia Walker Monash University Mr Miaoyi (Marvin) Wang Monash University Ms Qiaoyun Wang University of Queensland Mr Nicholas Westra van Holthe University of Queensland

Mr Nikolaos Andrikopoulos Monash University Mr Toby Funston University of New South Wales Mr Xumin Huang University of Queensland Mr Yuanbin Lu University of Queensland Mr Luke Marshall University of New South Wales Mr Xiao Tan University of Queensland Ms Xin Xu University of Queensland

Honours students Mr Kien Duong Monash University Ms Daniela Hidalgo Rocha University of Queensland Ms Jess Huang Monash University

Mr Marcin Wojnilowicz University of Melbourne

Ms Jia Kuah Monash University

Ms Johanna Wordsworth University of New South Wales

Ms Pooja Laxman University of New South Wales

Ms Leah Wright University of South Australia

Mr Francis McCallum University of Queensland

Ms Ching-min (Mindy) Yeh University of Queensland

Administrative Staff Dr Natalie Jones Chief Operating Officer Monash University and University of Queensland Ms Anne Meyer Senior Coordinator Communications and Events – until June 2019 Monash University Ms Samantha Snowden-Finch Senior Centre Administrator – April 2019 – June 2019 Senior Coordinator Communications and Events – from June 2019 Monash University Dr Charlotte Hurry Senior Centre Administrator – until April 2019 Monash University Mrs Cassie Statkevitch Senior Centre Administrator – from July 2019 Monash University Dr Maryline Chee Kimling Node Coordinator University of Melbourne

Mr Yuao Wu University of Queensland Ms Yunxin (Cindy) Xiao Monash University Ms Fangyun Xin Monash University Ms Yuen Yi (Jamie) Lam Monash University Mr Ken Yong Monash University Mr Aykut Zelcak Monash University

P E R FO R MAN C E

Ms Yijiao Qu University of Melbourne

Ms Nicole van der Burg University of Queensland

Ms Ruba Almasri University of South Australia

Mr Wenjie Zhang University of Melbourne

CBNS Annual Report 2019 75

Visitors to CBNS Associate Professor Hiroaki Akasaka Kobe University, Japan

Ms Rita Cimino University of Rome Tor Vergata, Italy

Professor Eric Bakker University of Geneva

Professor Jiwei Cui Shandong University, China

Ms Carolin Baur Heidelberg University, Germany

Professor Liming Dai University of New South Wales, Australia

Dr Mattias Bjornholm Imperial College London, UK

Dr Soraia Fernandes St Anne’s University Hospital Brno, Czechia

Miss Pauline Breddy CPE Lyon, France Dr Marco Cassani St Anne’s University Hospital Brno, Czechia Professor Chunying Chen National Center for Nanoscience and Technology of China, China Dr Rumei Cheng Wenzhou Medical College Mr Farouk Chorfi CPE Lyon, France

Professor Chunying Chen

76 CBNS Annual Report 2019

Miss Elisa Fine CPE Lyon, France Professor Amar Flood Indiana University Bloomington, USA Dr Pierre Gaudriault Cherry Biotech Dr Thomas Guerinier Cherry Biotech Ms Rui Guo Hunan University, China

Professor Ulrich G. Hoffman Freiberg Institute for Advanced Studies, Germany Ms Fitore Hoxha Eberhard Karls Universität Tübingen, Germany Professor Jing Hu Shanghai Institute of Technology, China Associate Professor Daisuke Ishii Nagoya Institute of Technology, Japan Professor Lei Jiang Chinese Academy of Sciences Professor Dayong Jin University of Technology Sydney, Australia Professor Norikato Kato Meiji University, Japan Miss Matilde Lacombe CPE Lyon, France Professor Lacey McNally University of Oklahoma, USA

Ms Anika Prinz Saarland University, Germany

Dr Mingyang Wang China Agricultural University, China

Ms Liviana Mummolo University of Bologna, Italy

Dr Bruno Sarmento University of Porto, Portugal

Professor Younan Xia Georgia University of Technology, Georgia

Ms Pataranapa Nimtrakul Naresuan University, Thailand

Mr Marco Savioli University of Rome Tor Vergata, Italy

Dr Yusuke Yonamine Hokkaido University, Japan

Professor David O’Connor University of Wiscosin–Madison, USA

Associate Professor Alejandro Sosnik Israel Institute of Technology

Mr Aykut Zelcek Izmir Institute of Technology, Turkey

Professor Shelby O’Connor University of Wiscosin–Madison, USA

Miss Beatrix Stadler Justus Liebig University, Germany

Professor Georg Papastavrou Bayreuth University, Germany

Professor Roland Stauber Universitätsmedizin Mainz, Germany

Dr Amanda Pearce University of Birmingham

Professor Pat Stayton University of Washington, USA

Mr Mitja Pohlen University of Ljubljana, Slovenia

Professor Sigridur Suman University of Iceland, Iceland

Professor Barbara Prainsack University of Vienna, Austria

Professor Wayne Tilley University of Adelaide

P E R FO R MAN C E

Ms Amalie Møller University of Copenhagen, Denmark

The Kempe Group

CBNS Annual Report 2019 77

Publications Books and Book Chapters Kuch D., Stringer N., Marshall L., Young S., Roberts M., Macgill I., Bruce A., Passey R., An energy data manifesto, 2019, Good Data, Institute of Networked Cultures McCarroll J.A., Sharbeen G., Kavallaris M., Phillips P.A., The use of star polymer nanoparticles for the delivery of siRNA to mouse orthotopic pancreatic tumor models, 2019, Methods in Molecular Biology Mitchell A.J., Ivask A., Ju Y., Quantitative measurement of cell-nanoparticle interactions using mass cytometry, 2019, Methods in Molecular Biology Siekmann I., Cao P., Sneyd J., Crampin E.J., Data-driven modelling of the InositolTrisphosphate Receptor (IP3R) and its role in Calcium-Induced Calcium Release (CICR), 2019, Computational Glioscience

Journal Entries Aghaei-Ghareh-Bolagh B., Guan J., Wang Y., Martin A.D., Dawson R., Mithieux S.M., Weiss A.S., Optically robust, highly permeable and elastic protein films that support dual cornea cell types, 2019, Biomaterials, 188, 50-62 Ahmad S.A.A., Ciampi S., Parker S.G., Gonçales V.R., Gooding J.J., Forming ferrocenyl self-assembled monolayers on Si(100) electrodes with different alkyl chain lengths for electron transfer studies, 2019, ChemElectroChem, 6(1), 211-220 Ahmed H., Rezk A.R., Richardson J.J., Macreadie L.K., Babarao R., Mayes E.L.H., Lee L., Yeo L.Y., Acoustomicrofluidic assembly of oriented and simultaneously activated metal–organic frameworks, 2019, Nature Communications, 10(1), 22820 Akhter D., Simpson J.D., Fletcher N.L., Houston N.L., Fuchs A.V., Bell C.A., Thurecht K.J., Oral delivery of multicompartment nanomedicines for colorecatal cancer therapeutics: combining loco-regional delivery with celltarget specificity, 2019, Advanced Therapeutics, 3(2), 19001710 Alam M.M., Jack K.S., Hill D.J.T., Whittaker A.K., Peng H., Gradient copolymers – Preparation, properties and practice, 2019, European Polymer Journal, 116(), 394-414 Albayaty Y.N., Thomas N., 78 CBNS Annual Report 2019

Jambhrunkar M., Al-Hawwas M., Kral A., Thorn C.R., Prestidge C.A., Enzyme responsive copolymer micelles enhance the antibiofilm efficacy of the antiseptic chlorhexidine, 2019, International Journal of Pharmaceutics, 566, 329-341 Alshawawreh F., Lisi F., Ariotti N., Bakthavathsalam P., Benedetti T., Tilley R.D., Gooding J.J., The use of a personal glucose meter for detecting procalcitonin through glucose encapsulated within liposomes, 2019, Analyst, 144(21), 6225-6230 Anand S.P., Prévost J., Baril S., Richard J., Medjahed H., Chapleau J.-P., Tolbert W.D., Kirk S., Smith A.B., Wines B.D., Kent S.J., Hogarth P.M., Parsons M.S., Pazgier M., Finzi A., Two families of env antibodies efficiently engage Fc-gamma receptors and eliminate HIV-1 -infected cells, 2019, Journal of Virology, 93(3), e01823-180 Arafeh-Dalmau N., MontañoMoctezuma G., Martinez J.A., Beas-Luna R., Schoeman D.S., Torres-Moye G., Extreme marine heatwaves alter kelp forest community near its equatorward distribution limit, 2019, Frontiers in Marine Science, 6, 4990 Aulsebrook M.L., Starck M., Grace M.R., Graham B., Thordarson P., Pal R., Tuck K.L., Interaction of nucleotides with a trinuclear terbium(iii)-dizinc(ii) complex: efficient sensitization of terbium luminescence by guanosine monophosphate and application to real-time monitoring of phosphodiesterase activity, 2019, Inorganic Chemistry, 58(1), 495-505 Bangaru S., Lang S., Schotsaert M., Vanderven H.A., Zhu X., Kose N., Bombardi R., Finn J.A., Kent S.J., Gilchuk P., Gilchuk I., Turner H.L., García-Sastre A., Li S., Ward A.B., Wilson I.A., Crowe J.E., A site of vulnerability on the influenza virus hemagglutinin head domain trimer interface, 2019, Cell, 177(5), 1136-1152 Bi C., Li X., Xin Q., Han W., Shi C., Guo R., Shi W., Qiao R., Wang X., Zhong J., Effect of extraction methods on the preparation of electrospun/electrosprayed microstructures of tilapia skin collagen, 2019, Journal of Bioscience and Bioengineering, 128(2), 234-240 Billings H., Wines B.D., Dyer W.B., Center R.J., Trist H.M., Kent S.J., Hogarth P.M., Boosting of markers of Fcγreceptor function in anti-HIV antibodies during structured treatment interruption, 2019, AIDS Research and Human

Retroviruses, 35(9), 842-852 Binte Abu Bakar S.Y., Salim M., Clulow A.J., Hawley A., Boyd B.J., Revisiting dispersible milk-drug tablets as a solid lipid formulation in the context of digestion, 2019, International Journal of Pharmaceutics, 554, 179-189 Booth S.G., Felisilda B.M.B., Alvarez De Eulate E., Gustafsson O.J.R., Arooj M., Mancera R.L., Dryfe R.A.W., Hackett M.J., Booth S.G., Secondary structural changes in proteins as a result of electroadsorption at aqueousorganogel interfaces, 2019, Langmuir, 35(17), 5821-5829 Boyd B.J., Bergström C.A.S., Vinarov Z., Kuentz M., Brouwers J., Augustijns P., Brandl M., Bernkop-Schnürch A., Shrestha N., Préat V., Müllertz A., BauerBrandl A., Jannin V., Successful oral delivery of poorly watersoluble drugs both depends on the intraluminal behavior of drugs and of appropriate advanced drug delivery systems, 2019, European Journal of Pharmaceutical Sciences, 137, 1049670 Cao E., Lindgren A., Martinsson S., Hu L., Lindfors L., Sigfridsson K., Skantze U., Michaëlsson E., Trevaskis N.L., Porter C.J.H., Promoting intestinal lymphatic transport targets a liver-X receptor (LXR) agonist (WAY252,623) to lymphocytes and enhances immunomodulation, 2019, Journal of Controlled Release, 296, 29-39 Castro J., Harrington A.M., Lieu T.M., Garcia-Caraballo S., Maddern J., Schober G., O’Donnell T., Grundy L., Lumsden A.L., Miller P., Ghetti A., Steinhoff M.S., Poole D.P., Dong X., Chang L., Bunnett N.W., Brierley S.M., Activation of pruritogenic TGR5, MRGPRA3, and MRGPRC11 on coloninnervating afferents induces visceral hypersensitivity, 2019, JCI Insight, 4(20), e1317120 Chen A., Er G., Zhang C., Tang J., Alam M., T. Ta H., Elliott A.G., Cooper M.A., Perera J., Swift S., Blakey I., Whittaker A.K., Peng H., Antimicrobial anilinium polymers: The properties of poly(N,Ndimethylaminophenylene methacrylamide) in solution and as coatings, 2019, Journal of Polymer Science, Part A: Polymer Chemistry, 57(18), 1908-1921 Chen J., Li J., Zhou J., Lin Z., Cavalieri F., Czuba-Wojnilowicz E., Hu Y., Glab A., Ju Y., Richardson J.J., Caruso F., Metal-phenolic coatings as a platform to trigger endosomal escape of nanoparticles, 2019, ACS Nano, 13(10), 11653-11664

Chuah K., Wu Y., Vivekchand S.R.C., Gaus K., Reece P.J., Micolich A.P., Gooding J.J., Nanopore blockade sensors for ultrasensitive detection of proteins in complex biological samples, 2019, Nature Communications, 10(1), 21090 Cirillo G., Curcio M., Spizzirri U.G., Vittorio O., Valli E., Farfalla A., Leggio A., Nicoletta F.P., Iemma F., Chitosan–quercetin bioconjugate as multi-functional component of antioxidants and dual-responsive hydrogel networks, 2019, Macromolecular Materials and Engineering, 304(5), 18007280 Cirillo G., Peitzsch C., Vittorio O., Curcio M., Farfalla A., Voli F., Dubrovska A., Iemma F., Kavallaris M., Hampel S., When polymers meet carbon nanostructures: expanding horizons in cancer therapy, 2019, Future medicinal chemistry, 11(16), 2205-2231 Cirillo G., Vittorio O., Kunhardt D., Valli E., Voli F., Farfalla A., Curcio M., Spizzirri U.G., Hampel S., Combining carbon nanotubes and chitosan for the vectorization of methotrexate to lung cancer cells, 2019, Materials, 12(18), 28890 Couturaud B., Houston Z.H., Cowin G.J., Prokeš I., Foster J.C., Thurecht K.J., O’Reilly R.K., Supramolecular fluorine magnetic resonance spectroscopy probe polymer based on passerini bifunctional monomer, 2019, ACS Macro Letters, 8(11), 1479-1483 Crawford T., Fletcher N., Veitch M., Cruz J.L.G., Pett N., Brereton I., Wells J.W., Mobli M., Tesiram Y., Bacillus anthracis protective antigen shows high specificity for a UV induced mouse model of cutaneous squamous cell carcinoma, 2019, Frontiers in Medicine, 6, 220 Cui J., Alt K., Ju Y., Gunawan S.T., Braunger J.A., Wang T.-Y., Dai Y., Dai Q., Richardson J.J., Guo J., Björnmalm M., Hagemeyer C.E., Caruso F., Ligand-functionalized poly(ethylene glycol) particles for tumor targeting and intracellular uptake, 2019, Biomacromolecules, 20(9), 3592-3600 Cui Y., Carosi J.M., Yang Z., Ariotti N., Kerr M.C., Parton R.G., Sargeant T.J., Teasdale R.D., Retromer has a selective function in cargo sorting via endosome transport carriers, 2019, Journal of Cell Biology, 218(2), 615-631 Dai Q., Yu Q., Tian Y., Xie X., Song A., Caruso F., Hao J., Cui J., Advancing metal-phenolic networks for visual information storage, 2019, ACS Applied Materials and Interfaces, 11(32), 29305-29311

Davenport M.P., Khoury D.S., Cromer D., Lewin S.R., Kelleher A.D., Kent S.J., Functional cure of HIV: the scale of the challenge, 2019, Nature Reviews Immunology, 19(1), 45-54 Deirram N., Zhang C., Kermaniyan S.S., Johnston A.P.R., Such G.K., pH-responsive polymer nanoparticles for drug delivery, 2019, Macromolecular Rapid Communications, 40(10), 18009170 Delon L.C., Guo Z., Oszmiana A., Chien C.-C., Gibson R., Prestidge C., Thierry B., A systematic investigation of the effect of the fluid shear stress on Caco-2 cells towards the optimization of epithelial organ-on-chip models, 2019, Biomaterials, 225(), 1195210 Deng C., Zhang Q., Fu C., Zhou F., Yang W., Yi D., Wang X., Tang Y., Caruso F., Wang Y., Templatefree synthesis of chemically asymmetric silica nanotubes for selective cargo loading and sustained drug release, 2019, Chemistry of Materials, 31(11), 4291-4298 Dening T.J., Joyce P., Kovalainen M., Gustafsson H., Prestidge C.A., Spray dried smectite clay particles as a novel treatment against obesity, 2019, Pharmaceutical Research, 36(1), 210 Dening T.J., Joyce P., Prestidge C.A., Improving correlations between drug solubilization and in vitro lipolysis by monitoring the phase partitioning of lipolytic species for lipid-based formulations, 2019, Journal of Pharmaceutical Sciences, 108(1), 295-304 Dey P., Thurecht K.J., Fredericks P.M., Blakey I., Tagged coresatellite nanoassemblies: role of assembling sequence on Surface-Enhanced Raman Scattering (SERS) performance, 2019, Applied Spectroscopy, 73(12), 3702819856660 Di Luca M., Curcio M., Valli E., Cirillo G., Voli F., Butini M.E., Farfalla A., Pantuso E., Leggio A., Nicoletta F.P., Tavanti A., Iemma F., Vittorio O., Combining antioxidant hydrogels with self-assembled microparticles for multifunctional wound dressings, 2019, Journal of Materials Chemistry B, 7(27), 4361-4370 Dong Y.-D., Tchung E., Nowell C., Kaga S., Leong N., Mehta D., Kaminskas L.M., Boyd B.J., Microfluidic preparation of drug-

loaded PEGylated liposomes, and the impact of liposome size on tumour retention and penetration, 2019, Journal of Liposome Research, 29(1), 1-9

dependent liposomal antigenspecific regulation of systemic inflammatory autoimmune disease, 2019, JCI Insight, 4(18), e1260250

a vasculogenesis microfluidic model for radiobiological studies of the human microvasculature, 2019, Advanced Materials Technologies, 4(4), 18007260

Fan S., Webb J.E.A., Yang Y., Nieves D.J., Gonçales V.R., Tran J., Hilzenrat G., Kahram M., Tilley R.D., Gaus K., Gooding J.J., Observing the reversible single molecule electrochemistry of Alexa Fluor 647 dyes by total internal reflection fluorescence microscopy, 2019, Angewandte Chemie – International Edition, 58(41), 14495-14498

Gao S., Hou J., Deng Z., Wang T., Beyer S., Buzanich A.G., Richardson J.J., Rawal A., Seidel R., Zulkifli M.Y., Li W., Bennett T.D., Cheetham A.K., Liang K., Chen V., Improving the acidic stability of zeolitic imidazolate frameworks by biofunctional molecules, 2019, Chem, 5(6), 1597-1608

Haque S., Feeney O., Meeusen E., Boyd B.J., McIntosh M.P., Pouton C.W., Whittaker M., Kaminskas L.M., Local inflammation alters the lung disposition of a drug loaded pegylated liposome after pulmonary dosing to rats, 2019, Journal of Controlled Release, 307, 32-43

Garvey C.J., Simon G.P., Whittaker A.K., Parker I.H., Moistureactivated dynamics on crystallite surfaces in cellulose, 2019, Colloid and Polymer Science, 297(4), 521-527

Hoang A.-P., Ruprai H., Fidanovski K., Eslami M., Lauto A., Daniels J., Mawad D., Porous and sutureless bioelectronic patch with retained electronic properties under cyclic stretching, 2019, Applied Materials Today, 15, 315-322

Farahani A.D., Martin A.D., Iranmanesh H., Bhadbhade M.M., Beves J.E., Thordarson P., Gel- and solid-state-structure of dialanine and diphenylalanine amphiphiles: importance of C⋅⋅⋅H interactions in gelation, 2019, ChemPhysChem, 20(7), 972-983 Faridi A., Sun Y., Mortimer M., Aranha R.R., Nandakumar A., Li Y., Javed I., Kakinen A., Fan Q., Purcell A.W., Davis T.P., Ding F., Faridi P., Ke P.C., Graphene quantum dots rescue protein dysregulation of pancreatic β-cells exposed to human islet amyloid polypeptide, 2019, Nano Research, 12(11), 2827-2834 Farrukh S.U.B., Javed I., Ather A.Q., Emwas A.-H., Alazmi M., Gao X., Chotana G.A., Davis T.P., Ke P.C., Saleem R.S.Z., Synthesis and identification of novel pyridazinylpyrazolone based diazo compounds as inhibitors of human islet amyloid polypeptide aggregation, 2019, Bioorganic Chemistry, 84, 339-346 Fong W.-K., Sánchez-Ferrer A., Rappolt M., Boyd B.J., Mezzenga R., Structural transformation in vesicles upon hydrolysis of phosphatidylethanolamine and phosphatidylcholine with phospholipase C, 2019, Langmuir, 35(46), 14949-14958 Fu C., Tang J., Pye A., Liu T., Zhang C., Tan X., Han F., Peng H., Whittaker A.K., Fluorinated glycopolymers as reductionresponsive 19F MRI agents for targeted imaging of cancer, 2019, Biomacromolecules, 20(5), 2043-2050 Galea R., Nel H.J., Talekar M., Liu X., Ooi J.D., Huynh M., Hadjigol S., Robson K.J., Ting Y.T., Cole S., Cochlin K., Hitchcock S., Zeng B., Yekollu S., Boks M., Goh N., Roberts H., Rossjohn J., Reid H.H., Boyd B.J., Malaviya R., Shealy D.J., Baker D.G., Madakamutil L., Richard Kitching A., O’Sullivan B.J., Thomas R., PD-L1– and calcitriol-

German J.B., Argov-Argaman N., Boyd B.J., Milk lipids: a complex nutrient delivery system, 2019, Nestle Nutrition Institute Workshop Series, 90, 217-225 Gerz I., Lindh E.M., Thordarson P., Edman L., Kullgren J., Mindemark J., Oligomer electrolytes for light-emitting electrochemical cells: influence of the end groups on ion coordination, ion binding, and turn-on kinetics, 2019, ACS Applied Materials and Interfaces, 11(43), 40372-40381 Gloag L., Mehdipour M., Chen D., Tilley R.D., Gooding J.J., Advances in the application of magnetic nanoparticles for sensing, 2019, Advanced Materials, 31(48), 19043850 Golani G., Ariotti N., Parton R.G., Kozlov M.M., Membrane curvature and tension control the formation and collapse of caveolar superstructures, 2019, Developmental Cell, 48(4), 523-538 Grace J.L., Amado M., Reid J.C., Elliott A.G., Landersdorfer C.B., Truong N.P., Kempe K., Cooper M.A., Davis T.P., Montembault V., Pascual S., Fontaine L., Velkov T., Quinn J.F., Whittaker M.R., An optimised cu(0)-rdrp approach for the synthesis of lipidated oligomeric vinyl azlactone: toward a versatile antimicrobial materials screening platform, 2019, Journal of Materials Chemistry B, 7(43), 6796-6809 Grassini D.R., da Silva J., Hall T.E., Baillie G.J., Simons C., Parton R.G., Hogan B.M., Smith K.A., Myosin Vb is required for correct trafficking of N-cadherin and cardiac chamber ballooning, 2019, Developmental Dynamics, 248(4), 284-295 Guo Z., Yang C.-T., Maritz M.F., Wu H., Wilson P., Warkiani M.E., Chien C.-C., Kempson I., Aref A.R., Thierry B., Validation of

Hong L., Dong Y.-D., Boyd B.J., Preparation of nanostructured lipid drug delivery particles using microfluidic mixing, 2019, Pharmaceutical Nanotechnology, 7(6), 484-495 Hong L., Sesen M., Hawley A., Neild A., Spicer P.T., Boyd B.J., Comparison of bulk and microfluidic methods to monitor the phase behaviour of nanoparticles during digestion of lipid-based drug formulations using: In situ X-ray scattering, 2019, Soft Matter, 15(46), 9565-9578 Hopkins J., Fidanovski K., Lauto A., Mawad D., Allorganic semiconductors for electrochemical biosensors: An overview of recent progress in material design, 2019, Frontiers in Bioengineering and Biotechnology, 7, 2370 Hopkins J., Travaglini L., Lauto A., Cramer T., Fraboni B., Seidel J., Mawad D., Photoactive organic substrates for cell stimulation: progress and perspectives, 2019, Advanced Materials Technologies, 4(5), 18007440 Huang X., Hu J., Li Y., Xin F., Qiao R., Davis T.P., Engineering organic/inorganic nanohybrids through RAFT polymerization for biomedical applications, 2019, Biomacromolecules, 20(12), 4243-4257 Islam J., Riley B.T., Fercher C., Jones M.L., Buckle A.M., Howard C.B., Cox R.P., Bell T.D.M., Mahler S., Corrie S.R., Wavelengthdependent fluorescent immunosensors via incorporation of polarity indicators near the binding interface of antibody fragments, 2019, Analytical Chemistry, 91(12), 7631-7638 Javed I., He J., Kakinen A., Faridi A., Yang W., Davis T.P., Ke P.C., Chen P., Probing the aggregation CBNS Annual Report 2019 79

P E R FO R MAN C E

Damelang T., Rogerson S.J., Kent S.J., Chung A.W., Role of IgG3 in infectious diseases, 2019, Trends in Immunology, 40(3), 197-211

and immune response of human islet amyloid polypeptides with ligand-stabilized gold nanoparticles, 2019, ACS Applied Materials and Interfaces, 11(11), 10462-10471 Javed I., Peng G., Xing Y., Yu T., Zhao M., Kakinen A., Faridi A., Parish C.L., Ding F., Davis T.P., Ke P.C., Lin S., Inhibition of amyloid beta toxicity in zebrafish with a chaperone-gold nanoparticle dual strategy, 2019, Nature Communications, 10(1), 37800 Jegaskanda S., Vanderven H.A., Tan H.-X., Alcantara S., Wragg K.M., Parsons M.S., Chung A.W., Juno J.A., Kent S.J., Influenza virus infection enhances antibody-mediated NK cell functions via type I interferondependent pathways, 2019, Journal of Virology, 93(5), e02090-180 Jia S., Tan A., Hawley A., Graham B., Boyd B.J., Visible lighttriggered cargo release from donor acceptor Stenhouse adduct (DASA)-doped lyotropic liquid crystalline nanoparticles, 2019, Journal of Colloid and Interface Science, 548, 151-159 Jiang D., Liang H., Yang W., Liu Y., Cao X., Zhang J., Li C., Liu J., Gooding J.J., Screen-printable films of graphene/CoS 2 /Ni 3 S 4 composites for flexible and arbitrary-shaped all-solid-state hybrid supercapacitor device, 2019, Carbon, 146, 557-567 Jiang W., Wragg K.M., Tan H.-X., Kelly H.G., Wheatley A.K., Kent S.J., Juno J.A., Identification of murine antigen-specific T follicular helper cells using an activation-induced marker assay, 2019, Journal of Immunological Methods, 467, 48-57 Jiang Z., Alam M.M., Cheng H.-H., Blakey I., Whittaker A.K., Spatial arrangement of block copolymer nanopatterns using a photoactive homopolymer substrate, 2019, Nanoscale Advances, 1(8), 3078-3085 Johnston S.T., Crampin E.J., Corrected pair correlation functions for environments with obstacles, 2019, Physical Review E, 99(3), 321240 Johnston S.T., Painter K.J., The impact of short- and longrange perception on population movements, 2019, Journal of Theoretical Biology, 460, 227-242 Joyce P., Dening T.J., Meola T.R., Gustafsson H., Kovalainen M., Prestidge C.A., Nanostructured clay particles supplement orlistat action in inhibiting lipid

80 CBNS Annual Report 2019

digestion: An in vitro evaluation for the treatment of obesity, 2019, European Journal of Pharmaceutical Sciences, 135, 1-11 Juno J.A., Phetsouphanh C., Klenerman P., Kent S.J., Perturbation of mucosalassociated invariant T cells and iNKT cells in HIV infection, 2019, Current Opinion in HIV and AIDS, 14(2), 77-84 Juno J.A., Wragg K.M., Amarasena T., Meehan B.S., Mak J.Y.W., Liu L., Fairlie D.P., McCluskey J., Eckle S.B.G., Kent S.J., MAIT cells upregulate α4β7 in response to acute simian immunodeficiency virus/simian HIV infection but are resistant to peripheral depletion in pigtail macaques, 2019, Journal of Immunology, 202(7), 2105-2120 Juno J.A., Wragg K.M., Kristensen A.B., Lee W.S., Selva K.J., Van Der Sluis R.M., Kelleher A.D., Bavinton B.R., Grulich A.E., Lewin S.R., Kent S.J., Parsons M.S., Modulation of the CCR5 receptor/ligand axis by seminal plasma and the utility of in vitro versus in vivo models, 2019, Journal of Virology, 93(11), e00242190 Kakinen A., Xing Y., Hegoda Arachchi N., Javed I., Feng L., Faridi A., Douek A.M., Sun Y., Kaslin J., Davis T.P., Higgins M.J., Ding F., Ke P.C., Single-molecular heteroamyloidosis of human islet amyloid polypeptide, 2019, Nano Letters, 19(9), 6535-6546 Kaminskas L.M., Williams C.C., Leong N.J., Chan L.J., Butcher N.J., Feeney O.M., Porter C.J.H., Tyssen D., Tachedjian G., Ascher D.B., A 30 kDa polyethylene glycol-enfuvirtide complex enhances the exposure of enfuvirtide in lymphatic viral reservoirs in rats, 2019, European Journal of Pharmaceutics and Biopharmaceutics, 137, 218-226 Kelly C.H.W., Benedetti T.M., Alinezhad A., Gooding J.J., Tilley R.D., Controlling metallic nanoparticle redox properties for improved methanol oxidation reaction electrocatalysis, 2019, ChemCatChem, 11(24), 59895993 Kelly H.G., Kent S.J., Wheatley A.K., Immunological basis for enhanced immunity of nanoparticle vaccines, 2019, Expert Review of Vaccines, 18(3), 269-280 Kent S.J., Davenport M.P., Moving the HIV vaccine field forward: concepts of protective immunity, 2019, The Lancet HIV, 6(6), E406-E410 Kesarwani V., Kelly H.G.,

Shankar M., Robinson K.J., Kent S.J., Traven A., Corrie S.R., Characterization of key bio-nano interactions between organosilica nanoparticles and Candida albicans, 2019, ACS Applied Materials and Interfaces, 11(38), 34676-34687 Khanna M., Jackson R.J., Alcantara S., Amarasena T.H., Li Z., Kelleher A.D., Kent S.J., Ranasinghe C. , Mucosal and systemic SIV-specific cytotoxic CD4+ T cell hierarchy in protection following intranasal/ intramuscular recombinant pox-viral vaccination of pigtail macaques, 2019, Scientific Reports, 9, 56610 Khaw K.-Y., Parat M.-O., Shaw P.N., Nguyen T.T.T., Pandey S., Thurecht K.J., Falconer J.R., Factorial design-assisted supercritical carbon-dioxide extraction of cytotoxic active principles from Carica papaya leaf juice, 2019, Scientific Reports, 9(1), 17160 Khiabani P.S., Soeriyadi A.H., Nam E.V., Peterson J.R., Webb J.E.A., Thordarson P., Donald W.A., Gooding J.J., Understanding the performance of a paperbased UV exposure sensor: The photodegradation mechanism of brilliant blue FCF in the presence of TiO2 photocatalysts in both the solid state and solution, 2019, Rapid Communications in Mass Spectrometry, 33(12), 1076-1083 Klein T., Parkin J., de Jongh P.A.J.M., Esser L., Sepehrizadeh T., Zheng G., De Veer M., Alt K., Hagemeyer C.E., Haddleton D.M., Davis T.P., Thelakkat M., Kempe K., Functional brush poly(2-ethyl2-oxazine)s: Synthesis by CROP and RAFT, thermoresponsiveness and grafting onto iron oxide nanoparticles, 2019, Macromolecular Rapid Communications, 40(10), 18009110 Klisuric A., Thierry B., Delon L., Prestidge C.A., Gibson R.J., Highlight Article: Identifying human and murine M cells in vitro, 2019, Experimental Biology and Medicine, 244(7), 554-564 Koach J., Holien J.K., Massudi H., Carter D.R., Ciampa O.C., Herath M., Lim T., Seneviratne J.A., Milazzo G., Murray J.E., McCarroll J.A., Liu B., Mayoh C., Keenan B., Stevenson B.W., Gorman M.A., Bell J.L., Doughty L., Hüttelmaier S., Oberthuer A., Fischer M., Gifford A.J., Liu T., Zhang X., Zhu S., Gustafson W.C., Haber M., Norris M.D., Fletcher J.I., Perini G., Parker M.W., Cheung B.B., Marshall G.M., Drugging MYCN

oncogenic signaling through the MYCN-PA2G4 binding interface, 2019, Cancer Research, 79(21), 5652-5667 Koziolek M., Alcaro S., Augustijns P., Basit A.W., Grimm M., Hens B., Hoad C.L., Jedamzik P., Madla C.M., Maliepaard M., Marciani L., Maruca A., Parrott N., Pávek P., Porter C.J.H., Reppas C., van Riet-Nales D., Rubbens J., Statelova M., Trevaskis N.L., Valentová K., Vertzoni M., Čepo D.V., Corsetti M., The mechanisms of pharmacokinetic food-drug interactions – A perspective from the UNGAP group, 2019, European Journal of Pharmaceutical Sciences, 134, 31-59 Kudisch M., Lim C.-H., Thordarson P., Miyake G.M., Energy transfer to Ni-amine complexes in dual catalytic, light-driven C-N crosscoupling reactions, 2019, Journal of the American Chemical Society, 141(49), 19479-19486 Ladd D., Tilūnaitė A., Roderick H.L., Soeller C., Crampin E.J., Rajagopal V., Assessing cardiomyocyte excitationcontraction coupling site detection from live cell imaging using a structurally-realistic computational model of calcium release, 2019, Frontiers in Physiology, 10, 12630 Lee W.S., Prévost J., Richard J., van der Sluis R.M., Lewin S.R., Pazgier M., Finzi A., Parsons M.S., Kent S.J., CD4- and timedependent susceptibility of HIV-1-infected cells to antibodydependent cellular cytotoxicity, 2019, Journal of Virology, 93(10), e01901-180 Lerra L., Farfalla A., Sanz B., Cirillo G., Vittorio O., Voli F., Grand M.L., Curcio M., Nicoletta F.P., Dubrovska A., Hampel S., Iemma F., Goya G.F., Graphene oxide functional nanohybrids with magnetic nanoparticles for improved vectorization of doxorubicin to neuroblastoma cells, 2019, Pharmaceutics, 11(1), 30 Lewis G.K., Ackerman M.E., Scarlatti G., Moog C., RobertGuroff M., Kent S.J., Overbaugh J., Reeves R.K., Ferrari G., Thyagarajan B., Knowns and unknowns of assaying antibodydependent cell-mediated cytotoxicity against HIV-1, 2019, Frontiers in Immunology, 10, 10250 Li K., Dai Y., Chen W., Yu K., Xiao G., Richardson J.J., Huang W., Guo J., Liao X., Shi B., Selfassembled metal-phenolic nanoparticles for enhanced

Fe/La-MOFs for drug delivery and dual-mode imaging, 2019, Journal of Materials Chemistry B, 7(42), 6612-6622

Li K., Xiao G., Richardson J.J., Tardy B.L., Ejima H., Huang W., Guo J., Liao X., Shi B., Targeted therapy against metastatic melanoma based on selfassembled metal-phenolic nanocomplexes comprised of green tea catechin, 2019, Advanced Science, 6(5), 18016880

Lin G., Rahim M.A., Leeming M.G., Cortez-Jugo C., Besford Q.A., Ju Y., Zhong Q.-Z., Johnston S.T., Zhou J., Caruso F., Selective metal-phenolic assembly from complex multicomponent mixtures, 2019, ACS Applied Materials and Interfaces, 11(19), 17714-17721

Li M., Chen T., Gooding J.J., Liu J., Review of carbon and graphene quantum dots for sensing, 2019, ACS Sensors, 4(7), 1732-1748 Li T., Clulow A.J., Nowell C.J., Hawley A., Cipolla D., Rades T., Boyd B.J., Controlling the size and shape of liposomal ciprofloxacin nanocrystals by varying the lipid bilayer composition and drug to lipid ratio, 2019, Journal of Colloid and Interface Science, 555, 361-372 Li T., Nowell C.J., Cipolla D., Rades T., Boyd B.J., Direct comparison of standard transmission electron microscopy and cryogenic-TEM in imaging nanocrystals inside liposomes, 2019, Molecular Pharmaceutics, 16(4), 17751781 Lian J., Yang Y., Wang W., Parker S.G., Gonçales V.R., Tilley R.D., Gooding J.J., Amorphous silicon on indium tin oxide: a transparent electrode for simultaneous light activated electrochemistry and optical microscopy, 2019, Chemical Communications, 55(1), 123-126 Liao H., Chen W., Dai Y., Richardson J.J., Guo J., Yuan K., Zeng Y., Xie K., Expression of programmed cell death-ligands in hepatocellular carcinoma: correlation with immune microenvironment and survival outcomes, 2019, Frontiers in Oncology, 9, 8830 Ligler F.S., Gooding J.J., Lighting up biosensors: now and the decade to come, 2019, Analytical Chemistry, 91(14), 8732-8738 Lim M., Puttick S., Houston Z.H., Thurecht K.J., Kalita-De Croft P., Mahler S., Rose S.E., Jeffree R.L., Mazzieri R., Dolcetti R., Lakhani S.R., Saunus J.M., Innovative therapeutic strategies for effective treatment of brain metastases, 2019, International Journal of Molecular Sciences, 20(6), 12800 Lin C., Chi B., Xu C., Zhang C., Tian F., Xu Z., Li L., Whittaker A.K., Wang J., Multifunctional drug carrier on the basis of 3d-4f

Liu Y., Tan H.-X., Koutsakos M., Jegaskanda S., Esterbauer R., Tilmanis D., Aban M., Kedzierska K., Hurt A.C., Kent S.J., Wheatley A.K., Cross-lineage protection by human antibodies binding the influenza B hemagglutinin, 2019, Nature Communications, 10(1), 3240 Lopez E., Scott N.E., Wines B.D., Hogarth P.M., Wheatley A.K., Kent S.J., Chung A.W., Low pH exposure during immunoglobulin G purification methods results in aggregates that avidly bind Fcγ receptors: Implications for measuring Fc dependent antibody functions, 2019, Frontiers in Immunology, 10, 24150 Lu M., Chen F., Cao C., Garvey C.J., Fletcher N.L., Houston Z.H., Lu H., Lord M.S., Thurecht K.J., Stenzel M.H., Importance of polymer length in fructosebased polymeric micelles for an enhanced biological activity, 2019, Macromolecules, 52(2), 477-486 Ma Y., Cortez-Jugo C., Li J., Lin Z., Richardson R.T., Han Y., Zhou J., Björnmalm M., Feeney O.M., Zhong Q.-Z., Porter C.J.H., Wise A.K., Caruso F., Engineering biocoatings to prolong drug release from supraparticles, 2019, Biomacromolecules, 20(9), 3425-3434 Ma Y., Wise A.K., Shepherd R.K., Richardson R.T., New molecular therapies for the treatment of hearing loss, 2019, Pharmacology and Therapeutics, 200, 190-209 Macnaghten P., Davies S.R., Kearnes M., Understanding public responses to emerging technologies: A narrative approach, 2019, Journal of Environmental Policy and Planning, 21(5), 504-518 Maghrebi S., Prestidge C.A., Joyce P., An update on polymerlipid hybrid systems for improving oral drug delivery, 2019, Expert Opinion on Drug Delivery, 16(5), 507-524 Makharza S.A., Cirillo G., Vittorio O., Valli E., Voli F., Farfalla A., Curcio M., Iemma F., Nicoletta

F.P., El-Gendy A.A., Goya G.F., Hampel S., Magnetic graphene oxide nanocarrier for targeted delivery of cisplatin: A perspective for glioblastoma treatment, 2019, Pharmaceuticals, 12(2), 760 Maqbool F., Moyle P.M., Thurecht K.J., Falconer J.R., Dispersibility of phospholipids and their optimization for the efficient production of liposomes using supercritical fluid technology, 2019, International Journal of Pharmaceutics, 563, 174-183 McLean M.R., Lu L.L., Kent S.J., Chung A.W., An inflammatory story: antibodies in tuberculosis comorbidities, 2019, Frontiers in Immunology, 10, 28460 McMahon K.-A., Wu Y., Gambin Y., Sierecki E., Tillu V.A., Hall T., Martel N., Okano S., Moradi S.V., Ruelcke J.E., Ferguson C., Yap A.S., Alexandrov K., Hill M.M., Parton R.G., Identification of intracellular cavin target proteins reveals cavin-PP1alpha interactions regulate apoptosis, 2019, Nature Communications, 10(1), 32790 Meiring J.C.M., Bryce N.S., Lastra Cagigas M., Benda A., Whan R.M., Ariotti N., Parton R.G., Stear J.H., Hardeman E.C., Gunning P.W., Colocation of Tpm3.1 and myosin IIa heads defines a discrete subdomain in stress fibres, 2019, Journal of cell science, 132(15) Mezzenga R., Seddon J.M., Drummond C.J., Boyd B.J., Schröder-Turk G.E., Sagalowicz L., Nature-inspired design and application of lipidic lyotropic liquid crystals, 2019, Advanced Materials, 31(35), 19008180 Mills R.J., Parker B.L., Monnot P., Needham E.J., Vivien C.J., Ferguson C., Parton R.G., James D.E., Porrello E.R., Hudson J.E., Development of a human skeletal micro muscle platform with pacing capabilities, 2019, Biomaterials, 198, 217-227 Mohd Amin M.C.I., Kiew L.V., Boyd B., Editorial, 2019, Drug Delivery and Translational Research, 9(2), 4170 Moles E., Kavallaris M., Fernàndez-Busquets X., Modeling the distribution of diprotic basic drugs in liposomal systems: Perspectives on malaria nanotherapy, 2019, Frontiers in Pharmacology, 10, 10640 Moreau D., Vacca F., Vossio S., Scott C., Colaco A., Paz Montoya J., Ferguson C., Damme M., Moniatte M., Parton R.G., Platt F.M., Gruenberg J., Drug-induced increase in lysobisphosphatidic acid reduces the cholesterol

overload in Niemann–Pick type C cells and mice, 2019, EMBO Reports, 20(7), e470550 Muller D.A., Depelsenaire A.C.I., Shannon A.E., Watterson D., Corrie S.R., Owens N.S., AgyeiYeboah C., Cheung S.T.M., Zhang J., Fernando G.J.P., Kendall M.A.F., Young P.R., Efficient delivery of dengue virus subunit vaccines to the skin by microprojection arrays, 2019, Vaccines, 7(4), 1890 Munir M.U., Ihsan A., Javed I., Ansari M.T., Bajwa S.Z., Bukhari S.N.A., Ahmed A., Malik M.Z., Khan W.S., Controllably biodegradable hydroxyapatite nanostructures for cefazolin delivery against antibacterial resistance, 2019, ACS Omega, 4(4), 7524-7532 Nicovich P.R., Lu X., Gaus K., Gooding J.J., Characterization of functionalized glass and indium tin oxide surfaces as substrates for super-resolution microscopy, 2019, Journal of Physics D: Applied Physics, 52(3), 340030 Nieves D.J., Hilzenrat G., Tran J., Yang Z., MacRae H.H., Baker M.A.B., Justin Gooding J., Gaus K., tagPAINT: Covalent labelling of genetically encoded protein tags for DNA-PAINT imaging, 2019, Royal Society Open Science, 6(12), 1912680 O’Mara P.B., Wilde P., Benedetti T.M., Andronescu C., Cheong S., Gooding J.J., Tilley R.D., Schuhmann W., Cascade reactions in nanozymes: Spatially separated active sites inside Ag-core-porous-Cu-shell nanoparticles for multistep carbon dioxide reduction to higher organic molecules, 2019, Journal of the American Chemical Society, 141(36), 14093-14097 Palmelund H., Andersson M. P., Asgreen C.J., Boyd B.J., Rantanen J., Löbmanna K., Tailor-made solvents for pharmaceutical use? Experimental and computational approach for determining solubility in deep eutectic solvents (DES), 2019, International Journal of Pharmaceutics, 1, 1000340 Pan S., Guo R., Richardson J.J., Berry J.D., Besford Q.A., Björnmalm M., Yun G., Wu R., Lin Z., Zhong Q.-Z., Zhou J., Sun Q., Li J., Lu Y., Dong Z., Banks M.K., Xu W., Jiang J., Jiang L., Caruso F., Ricocheting droplets moving on super-repellent surfaces, 2019, Advanced Science, 6(21), 19018460 Parsons M.S., Lee W.S., Kristensen A.B., Amarasena T., Khoury G., Wheatley A.K., Reynaldi A., Wines B.D., Hogarth P.M., Davenport M.P., Kent S.J., CBNS Annual Report 2019 81

P E R FO R MAN C E

synergistic combination therapy against colon cancer, 2019, Advanced Biosystems, 3(2), 18002410

Fc-dependent functions are redundant to efficacy of anti-HIV antibody PGT121 in macaques, 2019, Journal of Clinical Investigation, 129(1), 1224660 Partridge G.J., Rao S., Woolley L.D., Pilmer L., Lymbery A.J., Prestidge C.A., Bioavailability and palatability of praziquantel incorporated into solid-lipid nanoparticles fed to yellowtail kingfish Seriola lalandi, 2019, Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 218, 14-20 Patil R., Mohanty B., Liu B., Chandrashekaran I.R., Headey S.J., Williams M.L., Clements C.S., Ilyichova O., Doak B.C., Genissel P., Weaver R.J., Vuillard L., Halls M.L., Porter C.J.H., Scanlon M.J., A ligand-induced structural change in fatty acid– binding protein 1 is associated with potentiation of peroxisome proliferator–activated receptor agonists, 2019, Journal of Biological Chemistry, 294(10), 3720-3734 Peng H., Synthesis and application of fluorine-containing polymers with low surface energy, 2019, Polymer Reviews, 59(4), 739-757 Pham A., Gavin P.D., Libinaki R., Ramirez G., Khan J.T., Boyd B.J., Differential effects of TPM, a phosphorylated tocopherol mixture, and other tocopherol derivatives as excipients for enhancing the solubilization of co-enzyme Q10 as a lipophilic drug during digestion of lipidbased formulations, 2019, Current Drug Delivery, 16(7), 628-636 Pham T.T.T., Tran D.P., Thierry B., High performance indium oxide nanoribbon FETs: Mitigating devices signal variation from batch fabrication, 2019, Nanoscale Advances, 1(12), 4870-4877 Piya R., Zhu Y., Soeriyadi A.H., Silva S.M., Reece P.J., Gooding J.J., Micropatterning of porous silicon Bragg reflectors with poly(ethylene glycol) to fabricate cell microarrays: Towards single cell sensing, 2019, Biosensors and Bioelectronics, 127, 229-235 Pizzi D., Humphries J., Morrow J.P., Fletcher N.L., Bell C.A., Thurecht K.J., Kempe K., Poly(2oxazoline) macromonomers as building blocks for functional and biocompatible polymer architectures, 2019, European Polymer Journal, 121, 1092580 Pocock K., Delon L.C., Khatri A., Prestidge C., Gibson R., Barbe C., Thierry B., Uptake of silica

82 CBNS Annual Report 2019

particulate drug carriers in an intestine-on-a-chip: Towards a better: In vitro model of nanoparticulate carrier and mucus interactions, 2019, Biomaterials Science, 7, 2410-2420 Pu W., Nassar Z.D., Khabbazi S., Xie N., McMahon K.-A., Parton R.G., Riggins G.J., Harris J.M., Parat M.-O., Correlation of the invasive potential of glioblastoma and expression of caveolaforming proteins caveolin-1 and CAVIN1, 2019, Journal of NeuroOncology, 143(2), 207-220 Qiao Y., Shi C., Wang X., Wang P., Zhang Y., Wang D., Qiao R., Wang X., Zhong J., Electrospun nanobelt-shaped polymer membranes for fast and highsensitivity detection of metal ions, 2019, ACS Applied Materials and Interfaces, 11(5), 5401-5413 Ramírez-García P.D., Retamal J.S., Shenoy P., Imlach W., Sykes M., Truong N., Constandil L., Pelissier T., Nowell C.J., Khor S.Y., Layani L.M., Lumb C., Poole D.P., Lieu T.M., Stewart G.D., Mai Q.N., Jensen D.D., Latorre R., Scheff N.N., Schmidt B.L., Quinn J.F., Whittaker M.R., Veldhuis N.A., Davis T.P., Bunnett N.W., A pH-responsive nanoparticle targets the neurokinin 1 receptor in endosomes to prevent chronic pain, 2019, Nature Nanotechnology, 14, 1150-1159 Rezaei M., Winter M., ZanderFox D., Whitehead C., Liebelt J., Warkiani M.E., Hardy T., Thierry B., A reappraisal of circulating fetal cell noninvasive prenatal testing, 2019, Trends in Biotechnology, 37(6), 632-644 Rudd D.A., Benkendorff K., Chahal C., Guinan T., Gustafsson O.J.R., Esmaeelian B., Krysinska H., Pogson L., Voelcker N.H., Abbott C.A., Mapping insoluble indole metabolites in the gastrointestinal environment of a murine colorectal cancer model using desorption/ionisation on porous silicon imaging, 2019, Scientific Reports, 9(1), 123420 Ruprai H., Romanazzo S., Ireland J., Kilian K., Mawad D., George L., Wuhrer R., Houang J., Ta D., Myers S., Lauto A., Porous chitosan films support stem cells and facilitate sutureless tissue repair, 2019, ACS Applied Materials and Interfaces, 11(36), 32613-32622 Salim M., Fraser-Miller S.J., Sutton J.J., Berzinš K., Hawley A., Clulow A.J., Beilles S., Gordon K.C., Boyd B.J., Application of low-frequency Raman scattering spectroscopy to probe in situ drug solubilization in milk during digestion, 2019,

Journal of Physical Chemistry Letters, 10(9), 2258-2263 Salim M., Khan J., Ramirez G., Murshed M., Clulow A.J., Hawley A., Ramachandruni H., Beilles S., Boyd B.J., Impact of ferroquine on the solubilization of artefenomel (OZ439) during in vitro lipolysis in milk and implications for oral combination therapy for malaria, 2019, Molecular Pharmaceutics, 16(4), 1658-1668 Salim M., Ramirez G., Clulow A.J., Zhang Y., Ristroph K.D., Feng J., McManus S.A., Hawley A., Prud’Homme R.K., Boyd B.J., Solid-state behavior and solubilization of flash nanoprecipitated clofazimine particles during the dispersion and digestion of milk-based formulations, 2019, Molecular Pharmaceutics, 16(6), 27552765 Schultz H.B., Kovalainen M., Peressin K.F., Thomas N., Prestidge C.A., Supersaturated silica-lipid hybrid oral drug delivery systems: Balancing drug loading and in vivo performance, 2019, Journal of Pharmacology and Experimental Therapeutics, 370(3), 742-750 Selva K.J., Bavinton B.R., Grulich A.E., Pazgier M., Kelleher A.D., Kent S.J., Parsons M.S., Impact of HIV-1 viremia or sexually transmitted infection on semenderived anti-HIV-1 antibodies and the immunosuppressive capacity of seminal plasma, 2019, European Journal of Immunology, 49(12), 2255-2258 Selva K.J., Juno J.A., Worley M.J., Chung A.W., Tachedjian G., Kent S.J., Parsons M.S., Short communication: Effect of seminal plasma on functions of monocytes and granulocytes, 2019, AIDS Research and Human Retroviruses, 35(6), 553-556 Shi C., Bi C., Ding M., Xie J., Xu C., Qiao R., Wang X., Zhong J., Polymorphism and stability of nanostructures of three types of collagens from bovine flexor tendon, rat tail, and tilapia skin, 2019, Food Hydrocolloids, 93, 253-260 Siekmann I., Bjelosevic S., Landman K., Monagle P., Ignjatovic V., Crampin E.J., Mathematical modelling indicates that lower activity of the haemostatic system in neonates is primarily due to lower prothrombin concentration, 2019, Scientific Reports, 9(1), 39360 Siepmann J., Faham A., Clas S.-D., Boyd B.J., Jannin V., Bernkop-Schnürch A., Zhao H.,

Lecommandoux S., Evans J.C., Allen C., Merkel O.M., Costabile G., Alexander M.R., Wildman R.D., Roberts C.J., Leroux J.-C., Lipids and polymers in pharmaceutical technology: Lifelong companions, 2019, International Journal of Pharmaceutics, 558, 128-142 Simpson J.D., Ediriweera G.R., Howard C.B., Fletcher N.L., Bell C.A., Thurecht K.J., Polymer design and component selection contribute to uptake, distribution & trafficking behaviours of polyethylene glycol hyperbranched polymers in live MDA-MB-468 breast cancer cells, 2019, Biomaterials Science, 7(11), 4661-4674 Simpson J.D., Smith S.A., Thurecht K.J., Such G., Engineered polymeric materials for biological applications: Overcoming challenges of the bio-nano interface, 2019, Polymers, 11(9), 14410 Smith S.A., Selby L.I., Johnston A.P.R., Such G.K., The endosomal escape of nanoparticles: Toward more efficient cellular delivery, 2019, Bioconjugate Chemistry, 30(2), 263-272 Somers K., Wen V.W., Middlemiss S.M.C., Osborne B., Forgham H., Jung M.S., Karsa M., Clifton M., Bongers A., Gao J., Mayoh C., Raoufi-Rad N., Kusnadi E.P., Hannan K.M., Scott D.A., Kwek A., Liu B., Flemming C., Chudakova D.A., Pandher R., Failes T.W., Lim J., Angeli A., Osterman A.L., Imamura T., Kees U.R., Supuran C.T., Pearson R.B., Hannan R.D., Davis T.P., McCarroll J., Kavallaris M., Turner N., Gudkov A.V., Haber M., Norris M.D., Henderson M.J., A novel small molecule that kills a subset of MLL-rearranged leukemia cells by inducing mitochondrial dysfunction, 2019, Oncogene, 38(20), 3824-3842 Song D., Cui J., Ju Y., Faria M., Sun H., Howard C.B., Thurecht K.J., Caruso F., Cellular targeting of bispecific antibodyfunctionalized poly(ethylene glycol) capsules: Do shape and size matter?, 2019, ACS Applied Materials and Interfaces, 11(32), 28720-28731 Streck S., Clulow A.J., Nielsen H.M., Rades T., Boyd B.J., McDowell A., The distribution of cell-penetrating peptides on polymeric nanoparticles prepared using microfluidics and elucidated with small angle X-ray scattering, 2019, Journal of Colloid and Interface Science, 555, 438-448 Streck S., Hong L., Boyd B.J., McDowell A., Microfluidics for the production of nanomedicines:

Styles I.K., Feeney O.M., Nguyen T.-H., Brundel D.H.S., Kang D.W., Clift R., McIntosh M.P., Porter C.J.H., Removal of interstitial hyaluronan with recombinant human hyaluronidase improves the systemic and lymphatic uptake of cetuximab in rats, 2019, Journal of Controlled Release, 315, 85-96 Subramaniam S., Thomas N., Gustafsson H., Jambhrunkar M., Kidd S.P., Prestidge C.A., Rifampicin-loaded mesoporous silica nanoparticles for the treatment of intracellular infections, 2019, Antibiotics, 8(2), 390 Such G.K., Johnston A.P.R., Understanding cell interactions using modular nanoparticle libraries, 2019, Australian Journal of Chemistry, 72(8), 595-599 Sulistio A., Mansfeld F.M., ReyesOrtega F., D’Souza A.M., Ng S.M.Y., Birkett S., Blencowe A., Qiao G.G., Little C.B., Shu C.C., Bendele A.M., Valade D., Donohue A.C., Quinn J.F., Whittaker M.R., Davis T.P., Tait R.J., Intra-articular treatment of osteoarthritis with diclofenac-conjugated polymer reduces inflammation and pain, 2019, ACS Applied Bio Materials, 2(7), 2822-2832 Sun Y., Gu J., Wang H., Sessler J.L., Thordarson P., Lin Y.J., Gong H., AAAA-DDDD quadruple H-bond-assisted ionic interactions: Robust bis(guanidinium)/dicarboxylate heteroduplexes in water, 2019, Journal of the American Chemical Society, 141(51), 20146-20154 Sun Y., Kakinen A., Xing Y., Faridi P., Nandakumar A., Purcell A.W., Davis T.P., Ke P.C., Ding F., Amyloid self-assembly of hIAPP8-20 via the accumulation of helical oligomers, α-helix to β-sheet transition, and formation of β-barrel intermediates, 2019, Small, 15(18), 18051660

synuclein NACore, 2019, Nanoscale, 11(24), 1193311945 Suys E.J.A., Warren D.B., Pham A.C., Nowell C.J., Clulow A.J., Benameur H., Porter C.J.H., Pouton C.W., Chalmers D.K., A nonionic polyethylene oxide (PEO) surfactant model: Experimental and molecular dynamics studies of kolliphor EL, 2019, Journal of Pharmaceutical Sciences, 108(1), 193-204 Tan A., Lam Y.Y., Pacot O., Hawley A., Boyd B.J., Probing cell-nanoparticle (cubosome) interactions at the endothelial interface: Do tissue dimension and flow matter?, 2019, Biomaterials Science, 7(8), 3460-3470 Tan H.-X., Esterbauer R., Vanderven H.A., Juno J.A., Kent S.J., Wheatley A.K., Inducible bronchus-associated lymphoid tissues (IBALT) serve as sites of B cell selection and maturation following influenza infection in mice, 2019, Frontiers in Immunology, 10, 6110 Tan H.-X., Jegaskanda S., Juno J.A., Esterbauer R., Wong J., Kelly H.G., Liu Y., Tilmanis D., Hurt A.C., Yewdell J.W., Kent S.J., Wheatley A.K., Subdominance and poor intrinsic immunogenicity limit humoral immunity targeting influenza HA stem, 2019, Journal of Clinical Investigation, 129(2), 850-862 Tanaka J., Moriceau G., Cook A., Kerr A., Zhang J., Peltier R., Perrier S., Davis T.P., Wilson P., Tuning the structure, stability, and responsivity of polymeric arsenical nanoparticles using polythiol cross-linkers, 2019, Macromolecules, 52(3), 9921003 Tanaka J., Song J.-I., Lunn A.M., Hand R.A., Häkkinen S., Schiller T.L., Perrier S., Davis T.P., Wilson P., Polymeric arsenicals as scaffolds for functional and responsive hydrogels, 2019, Journal of Materials Chemistry B, 7(27), 4263-4271

Sun Y., Kakinen A., Xing Y., Pilkington E.H., Davis T.P., Ke P.C., Ding F., Nucleation of β-rich oligomers and β-barrels in the early aggregation of human islet amyloid polypeptide, 2019, Biochimica et Biophysica Acta - Molecular Basis of Disease, 1865(2), 434-444

Tang J., Li B., Howard C.B., Mahler S.M., Thurecht K.J., Wu Y., Huang L., Xu Z.P., Multifunctional lipid-coated calcium phosphate nanoplatforms for complete inhibition of large triple negative breast cancer via targeted combined therapy, 2019, Biomaterials, 216, 1192320

Sun Y., Kakinen A., Zhang C., Yang Y., Faridi A., Davis T.P., Cao W., Ke P.C., Ding F., Amphiphilic surface chemistry of fullerenols is necessary for inhibiting the amyloid aggregation of alpha-

Tang S.-Y., Mitchell D.R.G., Zhao Q., Yuan D., Yun G., Zhang Y., Qiao R., Lin Y., Dickey M.D., Li W., Phase separation in liquid metal nanoparticles, 2019, Matter, 1(1), 192-204

Tardy B.L., Richardson J.J., Nithipipat V., Kempe K., Guo J., Cho K.L., Rahim M.A., Ejima H., Caruso F., Protein adsorption and coordination-based endtethering of functional polymers on metal-phenolic network films, 2019, Biomacromolecules, 20(3), 1421-1428 Tee J.K., Yip L.X., Tan E.S., Santitewagun S., Prasath A., Ke P.C., Ho H.K., Leong D.T., Nanoparticles’ interactions with vasculature in diseases, 2019, Chemical Society Reviews, 48(21), 5381-5407 Teo J.L., Parton R.G., Yap A.S., The membrane environment of cadherin adhesion receptors: A working hypothesis, 2019, Biochemical Society Transactions, 47(4), 985-995 Thakur S.S., Chen Y.-S., Houston Z.H., Fletcher N., Barnett N.L., Thurecht K.J., Rupenthal I.D., Parekh H.S., Ultrasoundresponsive nanobubbles for enhanced intravitreal drug migration: An ex vivo evaluation, 2019, European Journal of Pharmaceutics and Biopharmaceutics, 136, 102-107 Thompson-Butel A.G., Shiner C.T., McGhee J., Bailey B.J., Bou-Haidar P., McCorriston M., Faux S.G., The role of personalized virtual reality in education for patients post stroke – a qualitative case series, 2019, Journal of Stroke and Cerebrovascular Diseases, 28(2), 450-457 Thordarson P., Beves J.E., Confining photoacidity, 2019, Chem, 5(6), 1366-1368 Tjandra K.C., Thordarson P., Multivalency in drug deliverywhen is it too much of a good thing?, 2019, Bioconjugate Chemistry, 30(3), 503-514 Tran D.P., Winter M., Yang C.-T., Stockmann R., Offenhäusser A., Thierry B., Silicon nanowires field effect transistors: a comparative sensing performance between electrical impedance and potentiometric measurement paradigms, 2019, Analytical Chemistry, 91(19), 1256812573 Turnbull T., Douglass M., Williamson N.H., Howard D., Bhardwaj R., Lawrence M., Paterson D.J., Bezak E., Thierry B., Kempson I.M., Crosscorrelative single-cell analysis reveals biological mechanisms of nanoparticle radiosensitization, 2019, ACS Nano, 13(5), 5077-5090 Turnbull T., Thierry B., Kempson

I., A quantitative study of intercellular heterogeneity in gold nanoparticle uptake across multiple cell lines, 2019, Analytical and Bioanalytical Chemistry, 411(28), 7529-7538 Vithani K., Goyanes A., Jannin V., Basit A.W., Gaisford S., Boyd B.J., A proof of concept for 3D printing of solid lipid-based formulations of poorly water-soluble drugs to control formulation dispersion kinetics, 2019, Pharmaceutical Research, 36(7), 1020 Vithani K., Goyanes A., Jannin V., Basit A.W., Gaisford S., Boyd B.J., An overview of 3D printing technologies for soft materials and potential opportunities for lipid-based drug delivery systems, 2019, Pharmaceutical Research, 36(1), 40 Vithani K., Jannin V., Pouton C.W., Boyd B.J., Colloidal aspects of dispersion and digestion of self-dispersing lipid-based formulations for poorly watersoluble drugs, 2019, Advanced Drug Delivery Reviews, 142, 16-34 Vogel Y.B., Gooding J.J., Ciampi S., Light-addressable electrochemistry at semiconductor electrodes: Redox imaging, mask-free lithography and spatially resolved chemical and biological sensing, 2019, Chemical Society Reviews, 48(14), 3723-3739 Vu M.N., Rajasekhar P., Poole D.P., Khor S.Y., Truong N.P., Nowell C.J., Quinn J.F., Whittaker M., Veldhuis N.A., Davis T.P., Rapid assessment of nanoparticle extravasation in a microfluidic tumor model, 2019, ACS Applied Nano Materials, 2(4), 1844-1856 Vu T.V., Tong H.D., Tran D.P., Binh N.T.T., Nguyen C.V., Phuc H.V., Do H.M., Hieu N.N., Electronic and optical properties of Janus ZrSSe by density functional theory, 2019, RSC Advances, 9(70), 41058-41065 Wang F., Barnes T.J., Prestidge C.A., Controlling and predicting the dissolution kinetics of thermally oxidised mesoporous silicon particles: towards improved drug delivery, 2019, Pharmaceutics, 11(12), 6340 Wang H., Ma Q., Qi Y., Dong J., Du X., Rae J., Wang J., Wu W.-F., Brown A.J., Parton R.G., Wu J.-W., Yang H., ORP2 delivers cholesterol to the plasma membrane in exchange for phosphatidylinositol 4, 5-bisphosphate (PI(4,5)P 2 ), 2019, Molecular Cell, 73(3), 458-473

CBNS Annual Report 2019 83

P E R FO R MAN C E

Considerations for polymer and lipid-based systems, 2019, Pharmaceutical Nanotechnology, 7(6), 423-443

Wang W., Gaus K., Tilley R.D., Gooding J.J., The impact of nanoparticle shape on cellular internalisation and transport: What do the different analysis methods tell us?, 2019, Materials Horizons, 6(8), 1538-1547

Wong J., Layton D., Wheatley A.K., Kent S.J., Improving immunological insights into the ferret model of human viral infectious disease, 2019, Influenza and other Respiratory Viruses, 13(6), 535-546

T.A., Deuterated phytantriol – A versatile compound for probing material distribution in liquid crystalline lipid phases using neutron scattering, 2019, Journal of Colloid and Interface Science, 534, 399-407

Guo J., Ju Y., Kempe K., Yarovsky I., Caruso F., Metal-dependent inhibition of amyloid fibril formation: Synergistic effects of cobalt-tannic acid networks, 2019, Nanoscale, 11(4), 1921-1928

Wei P., Gangapurwala G., Pretzel D., Leiske M.N., Wang L., Hoeppener S., Schubert S., Brendel J.C., Schubert U.S., Smart pH-sensitive nanogels for controlled release in an acidic environment, 2019, Biomacromolecules, 20(1), 130140

Xiao M., Hao M., Lyu M., Moore E.G., Zhang C., Luo B., Hou J., Lipton-Duffin J., Wang L., Surface ligands stabilized lead halide perovskite quantum dot photocatalyst for visible lightdriven hydrogen generation, 2019, Advanced Functional Materials, 29(48), 19056830

Yong K.W., Yuen D., Chen M.Z., Porter C.J.H., Johnston A.P.R., Pointing in the right direction: Controlling the orientation of proteins on nanoparticles improves targeting efficiency, 2019, Nano Letters, 19(3), 1827-1831

Weiss A.C.G., Krüger K., Besford Q.A., Schlenk M., Kempe K., Förster S., Caruso F., In situ characterization of protein corona formation on silica microparticles using confocal laser scanning microscopy combined with microfluidics, 2019, ACS Applied Materials and Interfaces, 11(2), 2459-2469

Xiao Y., Liu Q., Clulow A.J., Li T., Manohar M., Gilbert E.P., de Campo L., Hawley A., Boyd B.J., PEGylation and surface functionalization of liposomes containing drug nanocrystals for cell-targeted delivery, 2019, Colloids and Surfaces B: Biointerfaces, 182, 1103620

Zhao P., Pattison L.A., Jensen D.D., Jimenez-Vargas N.N., Latorre R., Lieu T., Jaramillo J.O., Lopez-Lopez C., Poole D.P., Vanner S.J., Schmidt B.L., Bunnett N.W., Protein kinase D and Gβγ mediate sustained nociceptive signaling by biased agonists of protease-activated receptor-2, 2019, Journal of Biological Chemistry, 294(27), 1064910662

White A.L., Langton C., Wille M.-L., Hitchcock J., Cayre O.J., Biggs S., Blakey I., Whittaker A.K., Rose S., Puttick S., Ultrasoundtriggered release from metal shell microcapsules, 2019, Journal of Colloid and Interface Science, 554, 444-452 Williams H.D., Ford L., Igonin A., Shan Z., Botti P., Morgen M.M., Hu G., Pouton C.W., Scammells P.J., Porter C.J.H., Benameur H., Unlocking the full potential of lipid-based formulations using lipophilic salt/ionic liquid forms, 2019, Advanced Drug Delivery Reviews, 142, 75-90 Winter M., Cai Z., Winkler K., Georgiou K., Inglis D., Lavranos T., Rezaei M., Warkiani M., Thierry B., Circulating tumour cell RNA characterisation from colorectal cancer patient blood after inertial microfluidic enrichment, 2019, MethodsX, 6, 1512-1520 Winter M., Gibson R., Ruszkiewicz A., Thierry B., Cytomorphological characterization of individual metastatic tumor cells from gastrointestinal cancer patient lymph nodes with imaging flow cytometry, 2019, Gastrointestinal Disorders, 1(4), 372-384 Wojnilowicz M., Glab A., Bertucci A., Caruso F., Cavalieri F., Superresolution imaging of proton sponge-triggered rupture of endosomes and cytosolic release of small interfering RNA, 2019, ACS Nano, 13(1), 187-202 Wong C.K., Stenzel M.H., Thordarson P., Non-spherical polymersomes: Formation and characterization, 2019, Chemical Society Reviews, 48(15), 40194035 84 CBNS Annual Report 2019

Xie W., Guo Z., Cao Z., Gao Q., Wang D., Boyer C., Kavallaris M., Sun X., Wang X., Zhao L., Gu Z., Manganese-based magnetic layered double hydroxide nanoparticle: A pH-sensitive and concurrently enhanced T1/T2weighted dual-mode magnetic resonance imaging contrast agent, 2019, ACS Biomaterials Science and Engineering, 5(5), 2555-2562 Yadav P., Zhang C., Whittaker A.K., Kailasam K., Shanavas A., Magnetic and photocatalytic curcumin bound carbon nitride nanohybrids for enhanced glioma cell death, 2019, ACS Biomaterials Science and Engineering, 5(12), 6590-6601 Yang C.-T., Xu Y., PourhassanMoghaddam M., Tran D.P., Wu L., Zhou X., Thierry B., Surface plasmon enhanced light scattering biosensing: Size dependence on the gold nanoparticle tag, 2019, Sensors (Switzerland), 19(2), 3230 Yang G., Liu Y., Wang H., Wilson R., Hui Y., Yu L., Wibowo D., Zhang C., Whittaker A.K., Middelberg A.P.J., Zhao C.-X., Bioinspired core–shell nanoparticles for hydrophobic drug delivery, 2019, Angewandte Chemie International Edition, 58(40), 14357-14364 Yee E.M.H., Cirillo G., Brandl M.B., Black D.S.C., Vittorio O., Kumar N., Synthesis of dextran–phenoxodiol and evaluation of its physical stability and biological activity, 2019, Frontiers in Bioengineering and Biotechnology, 7, 1830 Yepuri N.R., Clulow A.J., Prentice R.N., Gilbert E.P., Hawley A., Rizwan S.B., Boyd B.J., Darwish

Yu S.H., Esser L., Khor S.Y., Senyschyn D., Veldhuis N.A., Whittaker M.R., Ercole F., Davis T.P., Quinn J.F., Development of a shape-controlled H2S delivery system using epoxide-functional nanoparticles, 2019, Journal of Polymer Science, Part A: Polymer Chemistry, 57(18), 1982-1993 Yun G., Richardson J.J., Biviano M., Caruso F., Tuning the mechanical behavior of metalphenolic networks through building block composition, 2019, ACS Applied Materials and Interfaces, 11(6), 6404-6410 Zhang C., Li L., Han F.Y., Yu X., Tan X., Fu C., Xu Z.P., Whittaker A.K., Integrating fluorinated polymer and manganese-layered double hydroxide nanoparticles as pH-activated 19F MRI agents for specific and sensitive detection of breast cancer, 2019, Small, 15(36), 19023090 Zhang C., Sanchez R.J.P., Fu C., Clayden-Zabik R., Peng H., Kempe K., Whittaker A.K., Importance of thermally induced aggregation on 19F magnetic resonance imaging of perfluoropolyether-based comb-shaped poly(2-oxazoline)s, 2019, Biomacromolecules, 20(1), 365-374 Zhang J., Rogers F.J.M., Darwish N., Gonçales V.R., Vogel Y.B., Wang F., Gooding J.J., Peiris M.C.R., Jia G., Veder J.-P., Coote M.L., Ciampi S., Electrochemistry on tribocharged polymers is governed by the stability of surface charges rather than charging magnitude, 2019, Journal of the American Chemical Society, 141(14), 5863-5870 Zhang M., Gao W., Yan J., Wu Y., Marandino C.A., Park K., Chen L., Lin Q., Tan G., Pan M., An integrated sampler for shipboard underway measurement of dimethyl sulfide in surface seawater and air, 2019, Atmospheric Environment, 209, 86-91 Zhang W., Christofferson A.J., Besford Q.A., Richardson J.J.,

Zhao S., Caruso F., Dahne L., Decher G., De Geest B.G., Fan J., Feliu N., Gogotsi Y., Hammond P.T., Hersam M.C., Khademhosseini A., Kotov N., Leporatti S., Li Y., Lisdat F., LizMarzan L.M., Moya S., Mulvaney P., Rogach A.L., Roy S., Shchukin D.G., Skirtach A.G., Stevens M.M., Sukhorukov G.B., Weiss P.S., Yue Z., Zhu D., Parak W.J., The future of layer-by-layer assembly: A tribute to ACS Nano Associate Editor Helmuth Mohwald, 2019, ACS Nano, 13(6), 6151-6169 Zhong Q.-Z., Li S., Chen J., Xie K., Pan S., Richardson J.J., Caruso F., Oxidation-mediated kinetic strategies for engineering metal–phenolic networks, 2019, Angewandte Chemie International Edition, 58(36), 12563-12568 Zhou X., Yang C.-T., Xu Q., Lou Z., Xu Z., Thierry B., Gu N., Gold nanoparticle probe-assisted antigen-counting chip using SEM, 2019, ACS Applied Materials and Interfaces, 11(7), 6769-6776 Zhu W., Guo J., Amini S., Ju Y., Agola J.O., Zimpel A., Shang J., Noureddine A., Caruso F., Wuttke S., Croissant J.G., Brinker C.J., SupraCells: Living mammalian cells protected within functional modular nanoparticle-based exoskeletons, 2019, Advanced Materials, 31(25), 19005450 Zhu W., Guo J., Ju Y., Serda R.E., Croissant J.G., Shang J., Coker E., Agola J.O., Zhong Q.-Z., Ping Y., Caruso F., Brinker C.J., Modular metal–organic polyhedra superassembly: From molecularlevel design to targeted drug delivery, 2019, Advanced Materials, 31(12), 18067740

This report is printed on recycled paper. The text pages comprise 100% post-consumer waste and the cover stock comprises 60% post-consumer waste.

ARC Centre of Excellence in Convergent Bio-Nano Science and Technology Monash University, 399 Royal Parade, Parkville, Victoria, 3052, Australia Phone: +61 3 9903 9712 Email: info@cbns.org.au www.cbns.org.au

CBNS 2019 Annual Report

Articles inside

Publications

Awards, Memberships and Grant Success

CBNS Personnel

Performance and KPIs

Scientific Advisory Board

Governance Board

Media Coverage

CBNS Podcasts

Outreach Sub-Committee

CBNS Awards

Industry Partners Program Committee

Events

Education and Training Committee

Research Overview

Collaborations and Partnerships

Director’s Report

Strategic Plan

CBNS Experience

Overarching Projects

Strategic Projects

Cross-Centre Activity