Center for Advanced Research Technologies

Center for Advanced Research Technologies

Our mission is make the world stronger, more hopeful, and ever better by working with PI’s to explore and develop next-generation treatments and technologies.

CART.urmc.edu

The Center for Advanced Research Technologies (CART) is a collection of core facilities within URMC that share resources and expertise with our 3,000+ researchers and 500+ labs, and with principal investigators both inside and outside of the University of Rochester Medical Center.

Note from Our Director

Timothy Bushnell, PhD., MBA Director, CART Associate Professor, Pediatrics

The Center for Advanced Research Technologies (CART) is a collection of scientific resources necessary for performing successful biomedical research in today’s highly competitive environment. We work constantly to maintain state-of-the-art technology within our facilities in areas including microscopy and genomics, cytometry and mass spectrometry.

But the technology is only one component that CART provides.

The strength of CART is in our team of well-trained and highly-dedicated researchers whose job it is to focus on their chosen technology, so you don’t have to. When you meet with a member of CART, you get an expert who knows how their technology can help advance your research goals. You have a partner, every step of the way.

CART is there with you when it comes to reproducibility and rigor in biomedical research. We work hard to ensure that the data generated in our facilities is of the highest quality and meets the highest standards.

No matter where you are in your career, the CART team is here to support you, from experimental design to analysis of your data, leaving you to focus on your research goals. We look forward to meeting you and supporting your research.

CUTTING-EDGE

John M. Ashton, PhD., MBA Director, Genomics Research Center Assistant Professor, Biomedical Genetics

John Ashton has over 20 years of experience in genomics/genetics. Under his leadership, and with the operational support of Elizabeth Pritchett, PhD and the technical support of Jeff Malik, PhD, the Genomics Research Center is an integral facet of the URMC research community. John is passionate about advancing scientific discoveries and finding innovative solutions to complex research problems.

PUBLISHED

Our goal is to maximize

with principal investigators include:

• Platelet olfactory receptor activation limits

reactivity and growth of aortic aneurysms | PMID: 35324479

• Epigenetic aging of the demographically nonaging naked mole-rat | PMID: 35039495

• Coordination of endothelial cell positioning and fate specification by the epicardium | PMID: 34230480

• Modulation of the Human Pancreatic Ductal Adenocarcinoma Immune Microenvironment by Stereotactic Body Radiotherapy | PMID: 34862242

The

Flow Cytometry

Matthew Cochran, M.S. Technical Director, Flow Cytometry Resource Associate, Pediatrics

Our facility is fortunate to have access to a broad range of sophisticated technology that we can make available to University investigators, but getting the most out of that equipment can be a difficult task. In the FCR we’ve set up efficient and robust practices so we can provide high functionality and stability of operations. This allows our team to spend more time assisting our colleagues on advancing their science using flow cytometry. Whether a “simple” cell cycle assay, or a multi-year longitudinal study with numerous targets, our goal is to work with you to generate the best quality data possible.

PUBLISHED

Our published work includes:

• Development of an Orthotopic Murine Model of Rectal Cancer in Conjunction With Targeted ShortCourse Radiation Therapy | Advances in Radiation Oncology 2022 Mar-Apr; 7(2): 100867

• Lung megakaryocytes are immune modulatory cells | J Clinical Investigation 2021 Jan 4; 131(1): e137377

• In situ neutrophil efferocytosis shapes T

immunity to influenza infection | Nature IImmunology 2020 Sep;21(9):1046-1057

• FEI

• Gatan

• Transmission

• Ultramicrotomes

• Critical Point

• Glow Discharge

• Plunge Freezer,

• Light

• Stereo

Karen Bentley, M.S. Director, Electron Microscopy Resource Senior Associate, Pathology & Laboratory

Welcome to the Electron Microscopy Resource (EMR). As a faculty member in the Department of Pathology, researchers can benefit from my clinical EM background in the interpretation of their research specimens and to correlate their EM imaging study to light microscopy, immunofluorescence or confocal results. We invite you to email us for a Zoom consultation to discuss how Transmission-EM, Scanning-EM or Cryo-EM can advance your thesis project or grant- funded research.

Recent publications include:

• Periarteriolar spaces modulate cerebrospinal fluid transport into brain and demonstrate altered morphology in aging and Alzheimer’s disease | Nature Communications 2022

• Evidence of Staphylococcus Aureus Deformation, Proliferation, and Migration in Canaliculi of Live Cortical Bone in Murine Models of Osteomyelitis. J Bone Miner Res. 2017

Sina Ghaemmaghami, PhD Scientific Director, Mass Spectrometry Resource Associate Professor, Biology

The University of Rochester Medical Center’s Mass Spectrometry Research Laboratory (MSRL) is an established core facility that has provided a comprehensive suite of mass spectrometry services to investigators in Western New York for over 25 years. The core supports investigators with the planning, execution, analysis, and interpretation of proteomic and small molecule projects. We provide our

access to high-end instrumentation and complete consultation and analysis services to meet their specific project needs.

PUBLISHED

Our work with principal investigators include:

• Lin H, Tang M, Ji C, Girardi P, Cvetojevic G, Chen D, Koren SA, Johnson GVW. BAG3 Regulation of RAB35 Mediates the Endosomal Sorting Complexes Required for Transport/Endolysosome Pathway and Tau Clearance. Biol Psychiatry. 2022 Jul 1;92(1):10-24.

• Broly M, Polevoda BV, Al-Mamari W, Lim SC, Pais LS, Brown N, Riazuddin S, Bézieau S, Fu D, Isidor B, Cogné B, O’Connell MR. THUMPD1 biallelic variants cause loss of tRNA acetylation and a syndromic neurodevelopmental disorder. Am J Hum Genet. 2022 Apr 7;109(4):587-600.

V. Kaye Thomas, PhD Director, CALMN Assistant Professor, Pharmacology & Physiology

Welcome to the Center for Advanced Light Microscopy & Nanoscopy. In addition to providing access to high-end, state-of-the-art imaging equipment, the CALMN team endeavors to serve as a resource of imaging knowledge and experience. We provide hands-on, individual training to our researchers and can also guide scientists through experimental design, trouble-shooting and analysis phases of a project. Our aim is to form a partnership to support scientists in the research mission of the University, anticipating the future technological needs of the University community as we strive to embrace the value of Ever Better.

PUBLISHED

Recent publications include:

• Loss of function of renal Glut2 reverses hyperglycaemia and normalises body weight in mouse models of diabetes and obesity | PMID: 35290476

• CXCL10 peripheral activation niches couple preferred sites of Th1 entry with optimal APC encounter. | PMID: 34380032

• Perigenual and Subgenual Anterior Cingulate Afferents Converge on Common Pyramidal

in Amygdala Subregions of the Macaque | Journal of Neuroscience 2021

The BSL3 Core laboratory functions as a safe working environment for high-risk and high-security microbial pathogens. It is not a fee for service core, but functions instead as a core facility where properly trained users can study specific, Institutional Biosafety Committee-approved infectious agents under safe conditions, protecting both the user, other university personnel, and the public from harm. Users pay an hourly fee for use of the facility, which is charged out quarterly. The funds in the user account goes towards administrative fees, telecommunication charges, equipment service, and some user consumables such as certain components of personal protective equipment and safety and waste handling materials.

The Biosafety Level 3 (BSL3) core facility is available for use by any researcher whose work requires the manipulation of biological agents that may cause serious or potentially lethal disease as a result of exposure by the inhalation route.

Biosafety Level 3 Facility

CONTACT:

Martin S. Pavelka Jr., PhD Director, Biosafety Level 3 Facility Professor, Microbiology & Immunology

PUBLISHED

Our work with principal investigators include:

Raymonda MHCiesla JHMonaghan MLeach JAsantewaa GSmorodintsev-Schiller LALutz MMSchafer XLTakimoto TDewhurst SMunger JHarris IS Pharmacologic profiling reveals lapatinib as a novel antiviral against SARS-CoV-2 in vitro.; Virology; Vol 566. 2021 Nov 27.

Embong AK, Nguyen-Contant P, Wang J, Kanagaiah P, Chaves FA, Fitzgerald TF, Zhou Q, Kosoy G, Branche AR, Miller BL, Zand MS, Sangster MY, Topham DJ. “Formation

of Memory B Cells against Coronavirus in Acutely Infected COVID-19 Individuals.”

Pathogens.. 2022 Jan 29; 11(2)Epub 2022 Jan 29.