Suplemento do "XIX Pharmaceutical Congress of São Paulo" by Biblioteca do Conjunto das Químicas USP

The Brazilian Journal of Pharmaceutical Sciences is edited quartely by the Faculty of Pharmaceutical Sciences, University of São Paulo and it continues the “Revista Brasileira de Ciências Farmacêuticas/Brazilian Journal of Pharmaceutical Sciences”, which has its origin in the “Anais de Farmácia e Odontologia da USP”, that began on 1939. The Brazilian Journal of Pharmaceutical Sciences publishes research papers and reviews in all fields of Pharmaceutical Sciences and it reflects the advance that has been made in this area. It is mandatory that manuscripts submitted to BJPS have neither yet been published nor submitted simultaneously to any other journal. It is worth to note that the contents of the papers are exclusively the authors responsibility. Editorial Board The Editorial Committee, which determines the editorial policy of the BJPS, is composed of four members three of them elected by Faculty of Pharmaceutical Sciences of the University of São Paulo.

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Production Details Printed on acid-free paper. Brazilian Journal of Pharmaceutical Sciences, São Paulo, v.53, suppl.3, (2017) – São Paulo: Faculdade de Ciências Farmacêuticas, USP, 1999v., il.; 28cm. Trimestral Continuação de: Revista Brasileira de Ciências Farmacêuticas/ Brazilian Journal of Pharmaceutical Sciences, São Paulo, v.35, n.1, (1999). ISSN1984-8250 1. Farmácia. I.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas 615 CDD

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XIX Pharmaceutical Congress of São Paulo XI International Seminar of Pharmaceutical Sciences Expofar 2017

ABSTRACTS

October 06th to 08th, 2017 São Paulo – Brazil

Brazilian Journal of Pharmaceutical Sciences

PREFACE The XIX Pharmaceutical Congress of São Paulo, the XI International Seminar of Pharmaceutical Sciences and Expofar 2017 were held from October 06th to 08th, 2017, at the Frei Caneca Convention Center, in São Paulo, which were promoted by the São Paulo State Regional Council of Pharmacy (CRF-SP). Having as a central theme the “Pharmacist: Valued Professional Building Success”, the scientific agenda that was developed by a committee formed by renowned professors doctors and professionals, approached the several areas of pharmaceutical performance and promoted courses, lectures, symposia and round tables, offering the opportunity for a broad professional improvement. CRF-SP highlighted the importance of the scientific production in the pharmaceutical area and, again, in a partnership with the Brazilian Journal of Pharmaceutical Sciences (BJPS), publishes in this edition the successful scientific papers. The Professora Maria Aparecida Pourchet Campos Award, granted to the best abstracts, also attests the importance and the stimulus that CRF-SP gives to research and constant updating of the area. The Board of the São Paulo State Regional Council of Pharmacy (CRF-SP), in its constant search for the scientific improvement of the category, is proud of this work result and thank all those who were present and were involved and committed to this. Scientific Committee

Brazilian Journal of Pharmaceutical Sciences

CRF-SP BOARD President: Dr. Pedro Eduardo Menegasso Vice President: Dra. Raquel Rizzi General Secretary: Dr. Antonio Geraldo Ribeiro dos Santos Junior Chief Financial Officer: Dr. Marcos Machado Ferreira ORGANIZING COMMITTEE Dra. Terezinha de Jesus Andreoli Pinto (Coordinator) Dr. José Vanilton de Almeida (Honorary President) Dra. Akimi Mori Honda Dr. Dermeval de Carvalho Dra. Dirce Akamine Dra. Elfriede Marianne Bacchi Dra. Elvira Maria Guerra Shinohara Dr. Fabio Ribeiro da Silva Dr. Fernando de Sá Del Fiol Dr. Geraldo Alécio de Oliveira Dr. Lauro Domingos Moretto Dra. Mararrubia Botelho Dra. Marcia de Cássia Silva Borges Dra. Maria Fernanda Carvalho Dr. Mario Hirata Dr. Paulo Caleb Junior de Lima Santos Dr. Roberto Parise Filho Dra. Rosário Dominguez Crespo Hirata Dra. Silvia Storpirtis Sra. Christiane Matsunaga Sra. Marilia Cavalheri Casa Grande Sr. Renato Cintra Sra. Silmara Nunes D´Angelo

Brazilian Journal of Pharmaceutical Sciences

ABSTRACTS

Area 1 Pharmaceutical Care and Public Health

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP001 1,848 BRAZILIAN MELANOMA PATIENT CHARACTERISTICS: FROM DEMOGRAPHIC TO CLINICAL AND RELATED TO TREATMENT AND SURVIVAL FROM A SINGLE INSTITUTION IN BRAZIL TATIANA MAIA DA SILVA1; LETICIA MARIA MODES DA COSTA2; CAMILA SOUZA CROVADOR3; VINICIUS DE LIMA VAZQUEZ4. Unifeb, Barretos - SP - Brazil; 2Facisb, Guaira - SP - Brazil; 3Fundação Pio Xii, São José do Rio Preto - SP - Brazil; 4Fundação Pio Xii, Barretos - SP - Brazil. 1

Introduction and objective: Melanoma presents high lethality with higher incidence in recent years. The aim of this study was to characterize patient with melanoma population and associated with recurrence and survival. Materials and methods: Retrospective Cohort Study, information was collected from medical records of patients treated at the Barretos Cancer Hospital, classified according to demographic, histopathological, clinical, molecular and therapeutic characteristics. Results and conclusion: Data regarding 1848 patients were analyzed. The median age was 56.43 years. White skin was predominant with 93.1%. The prevalent subtype was superficial extensive with 649 cases (41.6%), and the anatomical location was the trunk in 606 cases (33.7%). Breslow thickness ranged from 0 to 70 mm (mean 3.40). The most common clinical stage was I (27.9%), followed by II (23.1%). Regarding the primary lesion, ulceration was present in 504 cases (42.6%) and the mean mitotic index was 4.5 mitosis/mm². The most common initial treatment was surgery (73.4%). Systemic treatment was carried out for 18.8% of patients, including Ipilimumab 16 patients (2.3%) and vemurafenib 19 patients (2.7%). The BRAF mutation was verified in 132 cases, with 42.4% positivity. The specific cancer survival for all patients in 5 years was 70.3%. The survival analysis showed that sex, TNM stage, and recurrence were significant prognostic factors. Clinical stage, as expected, presented high impact on the survival. This study characterizes the melanoma population of a referral hospital in the treatment of cancer in Brazil with patients’ real characteristics, allowing the creation of realistic treatment strategies, as well as the use of the database for future research. Financial support or fellowship: CLMM student research Fellowship grant 2015/19969-7 from FAPESP - São Paulo State Research Foundation - São Paulo Brazil; CFSP002 ABC CLASSIFICATION AND POSSIBLE REDUCTION OF COSTS IN A SECONDARY HOSPITAL IN FORTALEZA MARIA GORETI RODRIGUES JUVENAL LIMA1; TAIANA MAGALHÃES PIERDONÁ2. Undergraduate Student in Pharmacy at Faculdade Metropolitana da Grande Fortaleza Fametro, Fortaleza - CE - Brazil; 2Teacher of Faculty of Pharmacy at Faculdade Metropolitana da Grande Fortaleza Fametro, Fortaleza - CE - Brazil. 1

Introduction and objective: Managing health organization costs has a strategic role as, in this sector, financial resources become scarce over time and the treatment of diseases, more onerous. The ABC curve is a method of statistical classification of materials, which considers the importance of these items, based on the quantities used and their value. The objective of this study was to present an alternative approach to medication acquisition management in a hospital pharmacy at a secondary hospital in Fortaleza. Materials and methods: In this study, the ABC curve was used as a tool that allowed the establishment of important requirements related to the acquisition and inventory control of the drugs used in the institution and a diagnostic evaluation of costs. Considering that the study was performed in an emergency hospital with a large flow of surgeries, high consumption of antimicrobials (ATBs) and the relevance of these drugs to the institution, the ABC curve was performed for the ATBs in a period of three months (February to April 2017). The work was carried out by a student of the eighth semester of the Pharmacy degree during the discipline of Supervised Internship II and directed by teachers. Results and conclusion: The ABC curve identified a significant value invested in only 3 items of the ratio of ATBs. Ciprofloxacin, Meropenem and Oxacillin, comprised 79.3% of the total investment of the items applied on the ABC curve. The need to readjust the current standardization was perceived, as among the other drugs there is a possibility of substitution with a lower value (Levofloxacin), guided by prescribing professionals. Thus, it was concluded that the sector could establish exclusion criteria for items with less output, develop a program of rational use of drugs in curve A, in order to favor cost rationalization, since the ATBs represent the great majority of items. Other studies should still be carried out such as the application of the curve for the drugs of the directive 344 and hospital medical material (MMH).

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP003 ACTION OF HOMEOPATHIC MEDICINES ON THE “IN VITRO” GROWTH OF SACCHAROMYCES CEREVISIAE. INFLUENCE OF THE MEDICINAL PRODUCTS ON THE FERMENTATIVE ACTIVITY OF THE FUNGUS. VINICIUS CRESCENCIO QUEIROZ1; LEANDRO RIBEIRO BISSOLI2; ROSIMEIRE NAVICKAS CONSTANTINO DA SILVA2; ROBERTO LOPES DE ALMEIDA2; FERNANDO LUIS AFFONSO FONSECA2; TÂNIA AGUIAR PASSETI3. Fmabc - Faculdade de Medicina do Abc, Santo Andre - SP - Brazil; 2Fmabc - Faculdade de Medicina do Abc, Santo André - SP - Brazil; 3Fmabc, São Caetano do Sul - SP - Brazil. 1

Introduction and objective: We propose to study the action of homeopathic medicines Arnica Montanna, nosode of the fungus S. cerevisiae and Atropa belladonna on their in vitro growth, and the influence of these medicinal products on the fermentative characteristics of the fungus on crude raw beer wort. Materials and methods: 250 μL of each drug made in 30% alcohol were placed in tubes containing 5 mL of Saboraud (AS) agar or Beer Wort (BW) and 20 μL of fungus diluted to 0.5 of the Macfarland scale and then 1/100. Fungal growth was evaluated by spectrophotometry at 600nm or counting in Newbauer’s chamber. We evaluated the production of alcohol in the fermentation by the BRIX index, during 7 days of fermentation. After incubation of the fungus with the medicinal products, 1x107 fungi/mL were placed in 20 mL of new brewing mash. This was incubated at room temperature for 7 days. We again evaluated the growth of the fungus and the formation of alcohol. Results and conclusion: Results of in vitro fungal growth on AS media revealed that Arnica 30cH stimulated a significantly fungal growth, while nosodium 6 and 30cH produced a significant decrease in growth in fungi incubated with 30% alcohol. Concerning the production of alcohol in SA medium, we observed that Arnica 30 cH consumed a significantly lower amount of sugar, while the nosode 30cH consumed a greater amount of sugar in relation to the alcohol 30%. The results of yeast fungus growth with BW were similar to that of SA medium. Afterwards, these fungi were placed in 20 mL of BW at the concentration of 1x107 fungi/mL, and left incubating for 7 days. The results demonstrated that differences in fungal growth disappear, just as sugar consumption becomes homogenous. Demonstrating that the action of homeopathic medicines seems to exist only when it is in contact with the cell, not lasting after the environment exchange. Financial support or fellowship: NEPAS - Núcleo de Estudos, Pesquisas e Assessoria à Saúde da Faculdade de Medicina do ABC - FMABC (Brazil). CFSP004 ADHERENCE TO PHARMACOLOGICAL TREATMENT AND ASSOCIATED FACTORS IN A MEDIUM-SIZED MUNICIPALITY GEÓRGIA PELUCI DURANTE1; FELIPE ASSAN REMONDI2; CAMILO MOLINO GUIDONI1; ANA MARIA RIGO SILVA1; EDMARLON GIROTTO1. State University of Londrina, Londrina - PR - Brazil; 217th Regional Health Division in the State of Paraná, Londrina - PR - Brazil.

Introduction and objective: The increase in the prevalence of people with chronic non-communicable diseases is directly related to increased use of medications. In addition, this situation indicates possible problems with adherence to pharmacological treatment. The present study aims to analyze the adherence to pharmacological therapy and to identify the associated factors of residents of a medium-sized municipality in the north of Paraná, Brazil. Materials and methods: A cross-sectional population-based study conducted in 2015 with individuals over 44 years of age, in the city of Cambé, Paraná, Brazil. This study is part of the Vigicardio project, and involved residents who reported continued use of medications. Adherence to treatment was measured by Morisky’s 8-item Therapeutic Adhesion Scale (MMAS-8). The analysis of the data involved the calculation of the adjusted prevalence ratio, with a 95% confidence interval. Results and conclusion: A total of 563 subjects who were taking medication continuously, 64.7% female and 60.6 ± 10.2 years old, were evaluated. Regarding adherence to treatment, 308 (54.7%) were identified with high adherence and 45.3% with moderate or low adherence. After an adjusted analysis, individuals who reported more than two prescribing physicians (PR 1.199; 95% CI 1.021-1.407), who were unsatisfied with pharmacological treatment (PR 1.236, 95% CI 1.001-1.527) and not arterial hypertension (PR 2.206, 95% CI 1.980-2.458) had a higher prevalence of high adherence to treatment. It is concluded that the number of prescribers, satisfaction with the treatment and the presence of chronic disease affect adherence to pharmacological treatment, regardless of the adjustment variables used. Financial support or fellowship: National Council for Scientific and Technological Development (CNPq).

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP005 ADHESION TO TREATMENT AND KNOWLEDGE OF PHARMACOTHERAPY IN PATIENTS RECEIVING PHARMACEUTICAL CARE AT A SECONDARY CARE UNIT FRANCISCO HUGO LEITE DE OLIVEIRA ARNAUD; ALAN RODRIGUES DA SILVA; BEATRIZ MARIA DA SILVA BORGES; RENAN PEREIRA DE LIMA; MARIA ANGELINA DA SILVA MEDEIROS. Universidade de Fortaleza - Unifor, Fortaleza - CE - Brazil. Introduction and objective: Pharmaceutical care is a model of practice developed in the context of pharmaceutical care. It comprises the direct interaction of the pharmacist with the user, aiming at rational pharmacotherapy and obtaining definitive and measurable results, with the purpose of improving quality of life. Materials and methods: Analytical, cross-sectional exploratory study performed at a secondary care unit in Fortaleza. The Morisk instrument was used in order to evaluate the degree of adherence to pharmacotherapy. An adapted Delgado instrument was used to evaluate the degree of knowledge among the patients treated by the drug therapy. The present study was approved by the Ethics Committee for research on human beings of the University of Fortaleza, Certificate No. 134.041. Results and conclusion: The demographic, social and economic characteristics of the 83 patients included in the sample showed that 62.7% (n = 52) were females, with an average age of 55.5 ± 1.65 (x ± SEM). It was identified that 47% (n = 39) adhered to the prescribed medication treatment, 30.1% (n = 25) were not adherent unintentionally, while 22.9% (n = 19) were so intentionally. Directly related to the level of knowledge, where, of these 83 patients 54% (n = 45) presented a high level of knowledge about the basic items of the prescription, such as name, dose and frequency of administration. Financial support or fellowship: Financial support or fellowship: We are grateful for the support given by PET-SESU/MEC. CFSP006 ANALYSIS AND STRATEGIES FOR DRUG AVAILABILITY IN A HEALTH UNIT IN A CITY IN THE METROPOLITAN REGION OF SÃO PAULO-SP TATIA TIE YUHARA1; ROSA MALENA FAGUNDES XAVIER2; MAURO BIATO RODRIGUES BARBOSA3. Ufsc, Florianópolis - SC - Brazil; 2Universidade do Estado da Bahia-Uneb, Salvador - BA - Brazil; 3Universidade Nove de Julho, São Paulo - SP - Brazil. 1

Introduction and objective: The expansion of access to the public health system required changes in the organization of Pharmaceutical Care (AF) within the SUS, in order to enable the population to have access to essential medicines. This paper sets up the construction of the Operational Plan (PO) in order to analyze and propose strategies to minimize the lack of medicines in a health unit of a municipality of the metropolitan region of São Paulo-SP. Materials and methods: The preparation of the PO consisted of describing the problem and construction of herringbone, aiming to: 1-control the stock of medicines, avoiding expired validity or low monthly standard, avoiding the displacement of residents to other Pharmacies of Health Units or increasing the return physician; 2-instituting mechanisms tabular data pent-up demand to improve programming drug procurement and reducing the number of complaints by Pharmacy service users arising from lack of medicines and for each operation through actions necessary to achieve image-goal (availability of drugs). The next step consisted of analyzing the viability and feasibility before each action, and in case of deficits creating strategic activities to achieve them. Results and conclusion: The share strategies were created: adapt and/or release access to computerized dispensing system to generate data for certain drug dispensing period; greater Pharmacy and Therapeutic team performance in the analysis of prescription drugs, and raising the awareness of the municipal manager on the need for implementing electronic medical records with access to prescription drugs. The implementation process of the PO was an important resource to improve the process management unit, encouraging integration and intersectionality between the actors involved, with recognition of the strengths and weaknesses of the sector, enabling the targeting of actions for the qualification of AF.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP007 ANALYSIS OF ADVERSE DRUG REACTIONS RELATED TO THE USE OF ANTIRETROVIRAL DRUGS IN A CENTER OF REFERENCE IN INFECTIOUS DISEASES IN THE NORTH OF CEARÁ. RAQUELINE DE ABREU MARQUES1; CÍCERO IGOR SIMÕES MOURA SILVA2. Inta, Sobral - CE - Brazil; 2Inta, Fortaleza - CE - Brazil.

Introduction and objective: Introduction and objective: In the early 1980s a new disease came into the world’s limelight, HIVAIDs, which quickly spread across the globe, soon transforming into a global public health challenge with millions of fatal victims. Among many scientific developments on treating and managing HIV, antiretroviral therapy is probably the greatest. Objective: to analyze the adverse effects of antiretroviral therapy on patients managed at a referral center on infectious diseases located in the North of the State of Ceará. Materials and methods: Materials and methods: after approval by CEP/UVA (Protocol: 1.891.812) we conducted interviews by filling in structured questionnaires. Our sample consisted of 100 patients on antiretroviral-therapy over at least one month, aged 18 to 51 years, between December 2016 to March 2017. Results and conclusion: Results and conclusion: of the 100 respondents, 71% were male, while 22% were aged between 26-30 years and only 8% aged between 46-51 years. Most correspondents had full secondary education, while 5% were illiterate but only 4% possessed complete higher education. The adverse reactions found in researched subjects were compared to those specified on each drug’s leaflet. As expected the prevalence was higher: dizziness (85%), nausea (50%), dysgeusia (46%), insomnia (37%), nightmare (36%), asthenia (35%), mood swings and gastralgia (33%), headache and fatigue (31%), paresthesia (30%), diarrhea (28%). These effects impacted negatively on the quality of life of researched subjects. Among the combinations of HAART, 3TC+TDF+EFZ showed the highest probability for users to report adverse effects (96.5%), followed by EFZ+3TC+AZT (95.83%), LPV/r with 3TC+AZT (90.9%), ATV+RTV+3TC+TDF (75%), in that order. In brief, adverse effects were generally higher than data reported by drug-leaflets. CFSP008 ANALYSIS OF ANTIMICROBIAL CONSUMPTION IN ADULT ICU, IN THE PERIOD OF 2014-2016, IN THE CITY OF SÃO PAULO FERNANDA DOS SANTOS ZENAIDE 1; MILTON SOILBELMANN LAPCHIK 2; VALQUIRIA OLIVEIRA DE CARVALHO BRITO2; INGRID WEBER NEUBAUER2; MARIA DO CARMO SOUZA2; MARIA ANGELA KFOURI DE SOUTO GATTI TENIS2; MARIA GOMES VALENTE2. Núcleo Municipal de Controle de Infecção Hospitalar (Nmcih)/Ccd/Covisa, São Paulo - SP - Brazil; 2

Introduction and objective: The analysis of antimicrobial consumption is relevant information for the management of good practices in the rational use of antimicrobials and control measures against infection by multiresistant bacteria. Studies point to a temporal association between the higher consumption of antimicrobials and the worsening of microbial resistance in health care services. The use of antimicrobials in adult ICUs is monitored and analyzed by the NMCIH of the city of São Paulo, in support of the Program of Hospital Infection Control of the State of São Paulo. Objective: to analyze the consumption rates of the four most commonly consumed antimicrobials in an ICU of hospitals in the city of São Paulo. Materials and methods: CCIH and pharmacy professionals sent monthly data on the absolute consumption of antimicrobials in an adult ICU in an Excel spreadsheet. This data were transformed into antimicrobial consumption density based on the daily dosage (DDD), as the standards of the US Centers for Disease Control. The 2014-2016 spreadsheets were analyzed and the results of each indicator stratified by percentile 10%, 25%, 50% (median), 75% and 90%. The following antimicrobial consumption was analyzed: cefepime, cefotaxime, ceftazidime, ceftriaxone, ciprofloxacin, ertapenem, imipenem, levofloxacin, linezolide, piperaciline/tazobactam, polymyxin, teicoplanin, vancomycin, daptomycin, tigecycline, anphotericin B, caspofungin, fluconazole and micafungin. Results and conclusion: About 117 ICUs, corresponding to 94.3% of the total ICUs, participated in the study. The most commonly used antimicrobials were ceftriaxone, followed by meropenem, piperacillin/tazobactam, and vancomycin. The high and sustained consumption of antimicrobials, with a broader spectrum of action against antibiotic-resistant bacteria, serves as an alert for surveillance actions of the CCIH and clinical pharmacy in the adult ICUs of the city of SP, observing the recommendations for use, appropriate dosage and duration of treatment, as well as the opportunities for antibiotic descalonation, in support of procedures for the prevention and control of multiresistant bacterial infections in critical units.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP009 ANALYSIS OF BENZODIAZEPINE PRESCRIPTIONS DISPENSED IN AMBULATORY UNITS OF THE MUNICIPALITY OF GUARUJA IN THE PERIOD FROM SEPTEMBER TO NOVEMBER 2014. THAIRIS ALVES DE QUEIROZ; MARINA MARIA DE OLIVEIRA; PAULO ANGELO LORANDI. Universidade Católica de Santos, Santos - SP - Brazil. Introduction and objective: The study has the general objective of characterizing the practice of prescribing, dispensing, and the indiscriminate use of class (BZD) in Guarujá SP, in the period from September to November 2014. The data collected medicines from prescriptions, will be those defined as required by Ordinance 344/98. Materials and methods: Defined Daily Dose (DDD) was used to analyze rationality in the consumption of this therapeutic class in the city studied. A total of 2665 prescriptions of benzodiazepines in the discriminated period was found. Diazepam was the most prevalent, with 55.95%. Among the prescriptions, 2499 (93.77%) were not fully adequate to legal requirements. The medical specialty most represented was psychiatry (39%). Analyzing the consumption to DDD, 1.32 DDD/1000population/day, 0.56 DDD/Total people served/day and 0.49 DDD/total prescriptions met/day were found. As for consumption levels, 76.6% of patients were considered Level 1. Results and conclusion: It was found that 289 users were able to collect more than one prescription during the study period. The results pointed to prescription and dispensing failures. It is noteworthy that several patients had a high collection rate. This suggests that the absence of an integrated communication system between prescribers, dispensing units and managers, can increase the chance of developing dependence. The analysis of the consumption levels using the DDD parameter, given as reference, shows higher consumption in the city compared to other studies.

ANALYSIS OF PRESCRIPTION ERRORS OF ANTIMICROBIALS IN A COMMERCIAL DRUGSTORE

CFSP010

REBECA LEÃO CARVALHO; EDMAEL RODRIGUES DE MELO; JAMILLY KELLY OLIVEIRA NEVES. Maurício de Nassau College, Campina Grande - PB - Brazil. Introduction and objective: The medicine prescription is a document with legal value by which those who prescribe, dispense and administer medicine take responsibility for the patient and society. It is recognized that prescriptions have a singular role in medication error prevention and it is known that such errors may occur due to ambiguous, unreadable or incomplete prescriptions, causing serious harm to the patient. The aim of this study was to identify and quantify the most frequent types of prescription errors found in a private health establishment. Materials and methods: It was a cross-sectional study with a quantitative and descriptive approach. The sample is equivalent to all the antimicrobials prescriptions, during the period of February 01 - May 31 of 2017, in a commercial drugstore, situated in Ingá – PB. Results and conclusion: From the 1000 prescriptions analyzed, it was possible to observe that 100% presented the professional signature and stamp, however, the following errors were identified: patient age absence (100%), date absence (1%), incomplete dosage (4%), patient incomplete name (8.2%), illegibility (1.2%), and drug concentration absence (6%). The results present many prescription errors, especially in relation to medicine dosage, which can interfere in the patients’ correct treatment. Although there was a low percentage of illegible prescriptions, we cannot disregard the importance of prescriptions illegibility, as it is an essential factor to the security of medicine dispensing. This way, a constant evaluation of such occurrences is necessary in order to prevent them, thus guaranteeing more security and success in therapeutics. The druggist is fundamental in the process of medicine dispensing, contributing to its rational use, thus reducing medication errors and adverse events related to such errors, to provide more care quality, besides costs reduction.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP011 ANALYSIS OF THE ADEQUACITY OF INFORMATION CONTAINED IN ANTIMICROBIAL BULBS MARKETED IN BRAZIL, ABOUT DRUG-NUTRIENT INTERACTIONS TATYANA BARRETO DE CERQUEIRA MENEZES1; BIANCA VENAS DE ALMEIDA1; LAYSE DA SILVA OLIVEIRA1; ÍNGARA KEISLE SÃO PAULO BARRETTO2. Unidade de Ensino Superior de Feira de Santana, Feira de Santana - BA - Brazil; 2Unidade de Ensino Superior de Feira de Santana, Feira de Santana00 - BA - Brazil. 1

Introduction and objective: According to DRC 47/09 of ANVISA, the package insert is the document that contains technicalscientific and guiding information about medicines for their rational use and should describe drug interactions as well as inform drug-food interactions. Objective: Verify if the macrolides, quinolones and tetracycline inserts contain complete information on drug-nutrient interaction. Materials and methods: We analyzed the package inserts for health professionals of macrolides, quinolones and tetracyclines registered in ANVISA. We sought to verify the adequacy of information about drug-nutrient interactions. The information was compared with the literature. Results and conclusion: There is a lack of information about drug-nutrient interactions in the antimicrobial package inserts under study. Literature findings show that erythromycin should not be administered with acidic juices, soft drinks or carbonated beverages (REIS, 2004), while norfloxacin, ciprofloxacin and moxifloxacin are affected by ingestion of milk and derivatives (MOURA; REYES, 2002), and tetracyclines: doxycycline, minocycline and lymecycline, are capable of forming complexes with divalent and trivalent cations impairing their absorption (LOPES et al., 2013). However, only the tetracycline, norfloxacin, ciprofloxacin, vibramycin, minocycline and lymecycline package inserts present correct information on their food interactions. It is concluded that there is a need for adequate package inserts, informing drug-nutrient interactions and nutrient intake restrictions along with the administration of these drugs, so that the results can be maximized with the antimicrobial therapy adopted, contributing to use and minimizing therapeutic failures. Financial support or fellowship: UNEF CFSP012 ANALYSIS OF THE KNOWLEDGE OF WAITERS FROM BEACH STALLS WITH REGARD TO THE PREVENTION AND PROTECTION FROM SOLAR RAYS LUANA ARAÚJO SILVA1; OLGA SAMARA SILVA CAVALCANTE2; JOSENILDA MALVEIRA CAVALCANTI3. Undergraduate Student in Pharmacy at Faculdade Metropolitana da Grande Fortaleza – Fametro, Fortaleza - CE - Brazil; Undergraduate Student in Pharmacy at Universidade de Fortaleza - Unifor, Fortaleza - CE - Brazil; 3Professor of Faculty of Pharmacy at Faculdade Metropolitana da Grande Fortaleza – Fametro, Fortaleza - CE - Brazil. 1 2

Introduction and objective: Many studies relate solar radiation exposure as a major cause of skin cancer. The increasing incidence of skin cancer in populations located in risk areas is the cause of this study, such as waiters that work at beach stalls, exposed to solar radiation all day. This study also intends to disclose solar exposure prejudices, in order to stimulate healthy life habits, improving life quality. The goal of this study is the application of a survey to evaluate waiters’ knowledge regarding solar radiation protection, as well as to draw sociodemographic profile relating it to their knowledge level regarding skin cancer prevention. Materials and methods: This is a qualitative and quantitative descriptive study carried out through a sociodemographic survey application, based on solar exposure prevention habits of beach tents employees. This study took place at Fortaleza, Ceara at “Praia do Futuro” beach. Beach stall employees signed a free and informed consent form. Results and conclusion: 83% of those interviewed have knowledge about the damage caused by solar exposure. 100% of those interviewed declared they had already suffered solar burns and 73% of those interviewed suffer solar exposure on a daily basis, from 09h to 15h. Only 60% of those interviewed use sunblock and 56.7% affirmed that they usually forget to use sunblock. From the analysis of the data collected, it was possible to conclude that the sample group studied seems to be in a vulnerable condition, facing a possible chance of developing skin cancer.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP013 ANALYSIS OF THE USE OF ORAL ANTICOAGULANTS USED IN A PHILANTHROPIC HOSPITAL OF SALVADOR. LUANA DE CARVALHO SANTANA. Estácio - Fib, Salvador - BA - Brazil. Introduction and objective: Oral anticoagulation is an effective mechanism in the treatment and prevention of thromboembolic events. Its use has increased in recent years, because it is associated with the elevation of risk factors such as obesity, sedentary lifestyle and smoking, which may contribute to the occurrence of thromboembolic events. Although it offers a narrow therapeutic window, numerous pharmacological and alimentary interactions, and requires a more frequent control of INR, warfarin is the most prescribed anticoagulant. Because it is the oldest and most widely used anticoagulant in clinical trials, it offers greater safety in use, as well as being available in the Unified Health System, and is inexpensive to purchase. The new oral anticoagulants provide therapeutic comfort to the patient in relation to warfarin, but its use is still limited because it does not have as many studies as warfarin. Thus, this study was developed with the objective of proving the high prescription rate of warfarin in relation to the new standardized anticoagulants in the institution under study. Materials and methods: The research was conducted at a philanthropic hospital in Salvador-Bahia between January 2015 and December 2016. It is a retrospective quantitative study. After the identification of the prescription of standard anticoagulants used in this period, such as warfarin, Rivaroxabana, Dabigratana and Femprocumone, a quantitative analysis of the year 2015 was carried out and compared with the year 2016, in order to identify an increase or decrease in demand and the relationship between them. Results and conclusion: The number of prescriptions of Warfarin represents approximately 75% of prescriptions (6,360/8,444). Rivaroxabana presented 22% of the use, occupying the second position with an increase of 375 prescriptions in 2016 in relation to 2015. Dabigratana and Femprocumona together represent 3% of the use in the institution. Although Rivaroxabana demonstrates an increase in prescriptions compared to 2015, warfarin remains the pioneering prescription. CFSP014 ANTIMICROBIAL STABILITY AFTER RECONSTITUTION AND DILUTION: A FOCUS ON SAFE ADMINISTRATION IN A HOSPITAL ENVIRONMENT. GISELE ROMÃO PINTO1; ALESSANDRA COUTO BOAVA1; FABIANA DA SILVA FISNACK1; ANDRÉIA CRISTINA ZAGO DA SILVA1; CRISTIANE ELOÍSA VENÂNCIO GUEDES2. Universidade São Francisco, Bragança Paulista - SP - Brazil; 2Hospital Universitário São Francisco, Bragança Paulista - SP - Brazil. 1

Introduction and objective: It is estimated that 25 to 35% of hospitalized patients receive antimicrobials during hospitalization, which is equivalent to 30% of hospital pharmacy costs. In pediatrics, it is common to use a single vial for several doses or patients. Questions arise regarding the stability of the drug after reconstitution and dilution, considering stability variation among manufacturers. This research aimed to evaluate the standardized antimicrobials in a University Hospital to identify the variation of stability according to the manufacturer laboratory and improve the processes of reconstitution and storage, aiming at patient safety. Materials and methods: To collect data, the standardized antimicrobial package leaflets of the hospital were analyzed. Twentythree antimicrobials from sixteen different manufacturers were evaluated, totaling 68 leaflets. The data collected were compiled in an Excel sheet and made available to the nursing team. Results and conclusion: After analyzing the data it was observed that 70% of the package leaflets had a difference in stability among laboratories. Of these, 35% had post-reconstitution alterations and 65% had post-dilution alterations. This work demonstrates the need to rethink clinical practice and the benefits of developing collaborative studies between nursing and pharmacy, promoting quick access to technical information, to improve the practice of antimicrobial administration.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

ANTIMICROBIALS USE AND BACTERIAL RESISTANCE

CFSP015

REBECA LEÃO CARVALHO; EDMAEL RODRIGUES DE MELO; JAMILLY KELLY OLIVEIRA NEVES. Maurício de Nassau College, Campina Grande - PB - Brazil. Introduction and objective: the discovery of penicillin as the first antibiotic used with efficiency was the mark of antimicrobials in the world. Used in the period of the Second World War, when the cases of infections were alarming, the new antibiotic saved millions of people. The evolution of antimicrobials and indiscriminate use led to the emergence of more resistant microorganisms, nowadays, considered a global public health problem. The present study aimed to analyze the current situation of prescriptions in commercial drugstores, highlighting the classes of the most prescribed antimicrobials. Materials and methods: It is a cross-sectional and quantitative study, based on the analysis of 549 antimicrobials prescriptions, dispensed in May of 2017, in a health establishment, in the city of Ingá, PB, according to Collegiate Board Resolution (DRC) no. 20/2011. Results and conclusion: It was possible to conclude that 60.29% of the users were female. The prevalent age group were 0-12 years old in 21.86% of the cases, followed by 21-30 years old (20.95%) and over 60 years old (15.30%). The most prescribed medicine class was Penicillin with 26.98% of the prescriptions, followed by Quinolones (19.24%), Cephalosporins (15.47%), Macrolides (15.29%) and Sulfonamides (8.99%) of the prescriptions. We can conclude that the class of Penicillin remains the most prescribed throughout the years, however, the prototype has been little used, since the most part of the bacteria is already resistant to it. Preventive strategies in the resistance dissemination are essential and must be applied in all health ambits, in which awareness and attention work in pharmaceutics is necessary in order to propagate the effects that indiscriminate use of such drugs can cause. CFSP016 APPLICATION OF BEERS CRITERIA AND FRENCH CONSENSUS PANEL LIST IN THE IDENTIFYING POTENTIALLY INAPPROPRIATE MEDICINES IN ELDERLY OUTPATIENTS KARINA MARQUES BOTONO1; MARISA APARECIDA CROZARA2; VALTER LUIS DA COSTA JUNIOR1; LUCIANE MARIA RIBEIRO NETO1. Centro Universitário São Camilo, São Paulo - SP - Brazil; 2Hyalos Pharma Consulting and Training, São Paulo - SP - Brazil.

Introduction and objective: The elderly are the most exposed to polypharmacy with a high risk of adverse events related to the prescription of potentially inappropriate medication (PIM) for the elderly. The objective of this study was to identify the prevalence of prescription of PIM in outpatients aged 75 years and older treated at a Center for Promotion and Rehabilitation in Health and Social Integration, in the city of São Paulo. Materials and methods: The observational study evaluated the prescriptions of 20 patients aged 75 or over, all attended between April and June 2011 (CEP 127/07) and between September and December 2015 (CEP nº 1.166.812). Variables collected: age, gender, diagnosed diseases and medications used. The Beers Criteria, 2015 and French Consensus Panel List, 2007 (FCPL) were used to identify PIM. Results and conclusion: From the evaluation of the prescriptions of the 20 patients, there were 146 medicines in use. Regarding the Beers Criteria, 41 PIM (28.1%) were identified in 19 prescriptions (95%) and using the FCPL, 18 PIM (12.3%) were identified in 14 patient prescriptions (70%). Regarding Beers, one patient presented 5 PIM and only 1 presented no PIM. The prevalence was 1 PIM in 7 patients (35%). The most prescribed PIM was omeprazole (14.6%). Regarding the FCPL, 4 patients (20%) had 2 PIM, 10 patients (50%) had 1 PIM. The most prescribed PIM was cyclobenzaprine (22.2%). The most prescribed drug for the patients in the study was AAS (5.5%) and regarding the Beers Criteria, PIM was established in 2 cases due to age and dosage. The Beers Criteria and FCPL allowed the identification of PIM, and the former was more appropriate for the evaluation of patients’ prescriptions in this study, due to the greater number of PIM observed.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

ARTICULATED NETWORK IN THE IMPLANTATION OF PHYTOTHERAPY

CFSP017

ANA CLÁUDIA JORDÃO RODRIGUES. Prefeitura Municipal de Jundiaí, Jundiaí - SP - Brazil. Introduction and objective: Introduction: The National Policy of Medicinal Plants and Herbal Medicines has as a guideline to “promote and recognize the popular practices of using medicinal plants and home remedies” and the National Policy on Integrative and Complementary Practices, shows that the “strengthening and expanding popular participation and social control”, includes measures such as “rescue and value” this knowledge and “promote the exchange of information” between all involved, besides “stimulating the popular participation in the creation of orchards of medicinal species”. The UGPS and Pharmaceutical Care of Jundiai city develops actions based on the Objectives: Develop a relationship of trust and credibility between the population and Primary Health Care professionals, through Phytotherapy educational actions; Elect the Family Health Strategy (ESF) prioritizing the promotion, protection and recovery of health; Involve actions focused on intersectoriality, reaching a transethical dimension from the possibilities of creating new visions and establishing new values in the implementation of this Integrative and Complementary Practices program model. Materials and methods: Methodology: Training the team through Permanent Education; Cultivation of medicinal and aromatic plants with the intersectoral agreement of Municipal Development Unit through the Family Health Support Center and ESF; Community orientation workshops for social action and income generation in the Center of Coexistence and Reference Center for Social Assistance. Results and conclusion: Results and conclusion: Trained 70 professionals working in the Primary Health Care with thematic groups such as: Chronic Pain, Anxiety, and Diabetes. Offices in the Center of Coexistence and Reference Center for Social Assistance to empower the community regarding the recognition and forms of use of medicinal plants. Development of an informative primer on some medicinal plants with recommendation in Mental Health for including patients in the project of Pharmaceutical Care for the Management and Rational Use of Medicinal Plants. Intersectoral participation with the theme of Urban Agriculture.

ASPECTS RELATED TO NON-ADHERENT BEHAVIOR OF HYPERTENSIVE PATIENTS

CFSP018

TAMMYRYS NUTELS; ALFREDO DIAS DE OLIVEIRA FILHO; SABRINA JOANY FELIZARDO NEVES. Universidade Federal de Alagoas, Maceio - AL - Brazil. Introduction and objective: Arterial Hypertension is a highly prevalent clinical condition characterized by elevated and sustained blood pressure (BP) levels of 140/90 mmHg or greater. Although there is effective treatment for the control of hypertension, many patients have difficulties controlling their BP. Often the failure in treatment is related to modifiable factors, which lead us to Pseudoresistant Hypertension (PHTN). However, despite patients with resistant hypertension (RHTN), those with PHTN still do not have a well delineated profile. Thus, this study aimed to evaluate the impact of therapeutic adherence on PHTN. Materials and methods: This is a cross-sectional study of the second step of the research database: Prevalence of resistant and pseudoresistant hypertension in hypertensive patients followed by the Health Strategy of Family in Maceió -AL. Measurements of BP, application of forms for sociodemographic data collection, use of medications and therapeutic adherence were performed. The database was prepared in epiinfo 6.04 and analyzed in SPSS 13.0. Results and conclusion: The final sample consisted of 233 patients. We obtained 152 (65.2%) patients who had non-adherent behavior. The mean BP of adherent patients (135.8/83.0 mmHg) and non-adherent patients (138.8/85.0mmHg) to the antihypertensive treatment varied by about 5mmHg, however, the frequency of patients with controlled blood pressure among adherent patients was 60.0%, while among non-adherents the frequency fell to 40.6% Financial support or fellowship: FAPEAL

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP019 ASSESSMENT OF THE PROFILE AND ACCEPTANCE OF PHARMACEUTICAL INTERVENTIONS CARRIED OUT IN A NEONATAL ICU LUNA MAYRA DA SILVA E SILVA; DANIELLE FRANÇA FURTADO; FERNANDA BARRETO DA SILVA; PATRICIA BATISTA RIBEIRO CORRÊA; IARA ANTONIA LUSTOSA NOGUEIRA; LEANDRA MARLA AIRES TRAVASSOS VIANA. Hospital Universitário da Universidade Federal do Maranhão, São Luís - MA - Brazil. Introduction and objective: The clinical performance of the pharmacist presents growth and strengthening in the hospital routine, in order to guarantee safety in the use of medicines. The pharmacist has consolidated his space in the multiprofessional team, focusing on rational use of drugs and optimization of pharmacotherapy. Objective: To evaluate and measure the clinical performance of a team of pharmacists with an emphasis on the acceptance of interventions by the multiprofessional team of a Neonatal ICU in a University Hospital. Materials and methods: This is a cross-sectional, descriptive, retrospective study with quantitative and qualitative analysis of daily records of pharmaceutical interventions, in the period between July and December 2016. Results and conclusion: We evaluated 1956 prescriptions, of which 338 interventions were performed, which obtained 98.52% adherence. The main interventions were: reporting of interactions/incompatibilities (21.76%), reconstitution/dilution (15.29%), request for a restricted use form (14.71%), suggestion of substitution of therapy (9.11%), drug treatment time (4.71%) and medication dose (3.82%). The main interventions related to safe prescription were: reconstitution (63.41%), dose (15.85%) and suggestion of substitution of therapy (10.97%). The high rate of interventions proposed and the high acceptance rate of the pharmaceutical recommendations corroborate the relevance of the performance of the clinical pharmacist, evidencing the value of the service provided. The insertion of the pharmacist, with greater proximity in the line of care, decreases the risks from drug therapies, which contributes to patient safety and improves the quality of the treatment offered. CFSP020 ASSESSMENT OF RATIONAL ANTIMICROBIAL PROPHYLAXIS USE IN SURGERY ON ORTHOPEDIC AND TRAUMATOLOGY PATIENTS CÁSSIO FABRÍCIO DOS SANTOS; PEDRO GONÇALVES DE OLIVEIRA; ANA GISELA BONIFÁCIO; RAFAEL AFFINI MARTINS; MARIA STELLA PECCIN; RODRIGO SPINELI MACEDO. Unifesp, São Paulo - SP - Brazil. Introduction and objective: Surgical site infections (SSI) are the most common cause of extended hospitalization, increasing the costs of health care, morbidities and mortalities. There is evidence that antimicrobial prophylaxis implemented within one hour prior to incision can reduce microbial intraoperative contamination and ISC risks. Objective: To assess whether antimicrobial prophylaxis use in orthopedic and traumatology surgery was according to hospital Infection Control (IF) rules and the Clinical Practice Guidelines for Antimicrobial Prophylaxis in Surgery of 2013. Materials and methods: It was a prospective and descriptive study of 234 procedures with hospitalized patients between September and November 2015. The data were analyzed and antimicrobial prophylaxis rationality was performed based on coherence between recommended rules of IF and Clinical Practice Guidelines for Antimicrobial Prophylaxis in Surgery. Results and conclusion: 77.4% of surgeries had antimicrobial prophylaxis prior to surgery, 9.4% of these administered antibiotic more than once in the intraoperative, and the surgery average time was 217 minutes. The average time of 234 procedures varied between 15 to 450 minutes (average of 162 minutes), of which 13.2% took 240 minutes or more to be finalized; part of these, 16.12% had the second antibiotic dose administered only in the intraoperative. During the hospitalization period 93.5% had an antibiotic prescription and made use of it for an average 7.4. Non-compliance with hospital guidelines and Clinical Practice Guidelines for Antimicrobial Prophylaxis in Surgery attracted attention in the hospital studied. Likewise, it would be good to include a program of continuing education intermediated by infection doctors and pharmacists in order for the orthopedics to better follow the guidelines for antimicrobial prophylaxis in surgery to reduce waste and health risks for patients.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP021 ASSOCIATION BETWEEN DIABETES MELLITUS AND DEPRESSIVE SYMPTOMS IN THE BRAZILIAN POPULATION: POPULATION BASED STUDY CECILIA TORQUETI DE BARROS; CAUÊ PONTES BRIGANTI; LAURA JÚLIA ESQUITINI LOPES; TÂNIA REGINA FERREIRA; MARCUS TOLENTINO SILVA; CRISTIANE DE CÁSSIA BERGAMASCHI. Uniso, Sorocaba - SP - Brazil. Introduction and objective: Depression is twice as common in people with diabetes mellitus compared to the general population, and is considered one of the most neglected symptoms in these patients. Epidemiological studies approaching a prevalence of depressive symptoms in patients with diabetes mellitus were not found in Brazil. The objective of this study was to verify the prevalence of depressive symptoms in individuals with diabetes mellitus and elucidate the association between the disease and depressive symptoms in this population. Materials and methods: Data were collected from the National Health Survey conducted in 2013, a cross-sectional populationbased study that contains information on 60,202 individuals interviewed. The study participants were selected using three-stage simple randomized conglomerate sampling: census tracts, households and residents aged 18 years old and over. The mean values of PHQ-9 were calculated for the variables studied and Tobit regression was used to evaluate their variation in these individuals. Results and conclusion: The prevalence of the Brazilian population with diabetes mellitus was 6.03%. There was a predominance of this disease in women, elderly individuals, widows, incomplete primary education and obese individuals. The severity of depressive symptoms was higher in women, the elderly, indigenous ethnic groups, divorced and widowed, with incomplete primary education and in obese individuals. Insulin use was associated with increased severity of depressive symptoms. Individuals with the comorbidities (heart attack, presence of diabetic foot, with amputation of limbs, who presented with diabetic coma and with circulatory problems) and those hospitalized due to the disease presented higher severity of depressive symptoms. Conclusion: Interventions that improve depressive symptoms in individuals with diabetes mellitus should prioritize women, the elderly, indigenous, widowed, separated/divorced, incomplete elementary school and obese individuals. Financial support or fellowship: This project is funded by governmental Program Graduate Education Institutions - PROSUP - CAPES/UNISO

ASSOCIATION BETWEEN HEALTH LITERACY AND MEDICATION ADHERENCE

CFSP022

ANDRESSA LEITE LEÃO1; SABRINA JOANY FELIZARDO NEVES2; ALFREDO DIAS DE OLIVEIRA FILHO1. School of Nursing and Pharmacy (Esenfar), Federal University of Alagoas, Maceió - AL - Brazil; 2School of Nursing and Pharmacy (Esenfar), Federal University of Alagoas. 1

Introduction and objective: Health literacy is a measure highly associated with health outcomes and often linked to age and education. Hypertension is a common medical condition that requires consistent adherence to antihypertensive treatment to improve physiological and morbimortality outcomes. Our study aimed to determine the association between medication adherence and health literacy in hypertensive outpatients Materials and methods: A cross-sectional study was carried out with hypertensive patients aged ≥18 years old treated at three of the Basic Health Units in Maceió, Brazil, through interviews and home blood pressure measurements, between May and October 2015. Medication adherence was determined by 8-item Morisky Medication Adherence Scale (MMAS-8) Portuguese version. Health literacy was assessed with the Medical Term Recognition Test (METER). The complexity of antihypertensive pharmacotherapy was measured by Medication Regimen Complexity Index (MRCI). Results and conclusion: A total of 200 patients were included in the study. The mean age of patients was 58.2 years, and the majority (82%) of them were females. Only 34.5% had controlled BP. The prevalence of medication adherence was 48%, while 21.5% had good HL. Age, schooling and physical activity positively influenced medication adherence. HL was higher among patients with higher educational level, number of antihypertensive drugs and higher MRCI scores. MMAS-8 scores were significantly higher among patients with good health literacy. A significant connection was not observed between health literacy and medication adherence. Financial support or fellowship: FAPEAL

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

ASSOCIATION BETWEEN OVERWEIGHT AND PSEUDORESISTANT HYPERTENSION

CFSP023

ROSILEIDE ZEFERINO DA SILVA; ALFREDO DIAS DE OLIVEIRA FILHO; SABRINA JOANY FELIZARDO NEVES. Ufal, Maceió - AL - Brazil. Introduction and objective: Systemic arterial hypertension (SAH) is a chronic disease characterized by high and sustained blood pressure levels. Pseudoresistance in patients with hypertension is associated with some factors that lead to false resistance of blood pressure levels. To achieve successful treatment, both pharmacological and non-pharmacological measures are essential, this implies behavioral change, adherence to treatment and a healthy eating plan. Body weight loss is associated with reductions in BP in overweight people. This study aimed to identify the relation between pseudorestistant hypertension and overweight. Materials and methods: A cross-sectional study was carried out with hypertensive patients older than 18 years, treated at one Family Health Strategy Unit in Maceió (AL), through interviews and blood pressure measurements, during 2015 and 2016. The following variables were investigated: gender, age, BMI, medications, dosage, time of use of antihypertensives, and systolic and diastolic pressures. The study was approved by the Ethics Committee in Research of Universidade Federal de Alagoas, certificate No. 19148713100005013. Data were collected only after the informed consent had been signed by all patients. Results and conclusion: Of the 247 subjects included in this study, 178 (72.06%) were female. The frequency of hypertensive patients who were not-adherent to medication corresponded to 60.3% and uncontrolled blood pressure levels were 46.3%. From these data, the patients were classified in patients with pseudoresistant hypertension 102 (39.7%), Body mass index (BMI) average was 29.22 kg/m². Overweight was more common among patients with pseudoresistant hypertension, with a frequency of 70.2% (42) compared with non-pseudoresistant hypertension, which presented 29.8% (17). The average blood pressure in the overweight patients was increased by 9 mmHg in systolic blood pressure (SBP) and 7 mmHg in diastolic blood pressure (DBP). Patients with pseudoresistant hypertension have a higher frequency of overweight and have higher blood pressure means than others. Financial support or fellowship: FAPEAL; CAPES CFSP024 ASSOCIATION OF MOTOR IMAGERY TO PHYSICAL TRAINING IN ELDERLY PEOPLE WITH PARKINSON’S DISEASE AS COMPLEMENTARY THERAPY PRISCILLA DE DIO SANTOS PONDÉ. Universidade São Judas Tadeu, São Paulo - SP - Brazil. Introduction and objective: With the aging population, the prevalence of chronic degenerative diseases such as Parkinson’s disease increases (PD), thus, programs that preserve the functional capacity, promoting the quality of life, become necessary. To this end, integrative and complementary practices are supportive of pharmacological treatment. Despite the functional decline in PD, new neural connections can be established or preserved through different interventions. Physical training associated with imagery is promoting factors of neuroplasticity. Objectives: To evaluate improvement in daily life activities and body perception after intervention of motor imagery associated with physical training. Materials and methods: Through this quantitative research, the variables were evaluated pre- and post-intervention. After approval of the project (CAAE 56852116.2.0000.0089), the subjects were submitted to the Mental State Mini Exam (MEEM - mean score 27.7) and questionnaire. Before and after evaluations of the Body Perception Index (BPI) and the score in the activities of daily living (ADL - basic and instrumental) were referenced in the scientific literature, subjects were submitted to the practice of motor imagery associated with aerobic physical training in running machine. Results and conclusion: 13 subjects with Parkinson’s disease were included. Of these, 8 completed all stages of the study. The mean age was 60.6 years; they were all males and Caucasian; 75% married, 12.5% separated and 12.5% widowed; 37.5% with education between 5 and 8 years and 62.5% with more than 9 years; 50% with family income between 2 and 4 minimum wages and the other 50%> 4. The BPI was evaluated before with an average score of 107.9% and after the intervention, 103.5%, representing a greater approximation between perception and real value. The basic ADL’s went from mild dependency (5.3) to total independence (5.8); Instruments ADL’s from 5.9 to 6.6, characterizing subjects as independent. This study is underway and other variables (serum levels of BDNF - Brain Derived Neurotrophic Factor and laterality tests) will still be analyzed in order to obtain data that further corroborate the theory of neuroplasticity in the search to associate this complementary therapy to pharmacological treatment.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

BURNOUT AND PSYCHOTROPIC DRUGS IN PRIMARY HEALTH CARE PROFESSIONALS

CFSP025

RAMONIELLE ALVES DOS SANTOS; MARCIA HELENA MIRANDA CARDOSO. Universidade Federal de Alfenas, Alfenas - MG - Brazil. Introduction and objective: Currently, in services where there is a caring relationship with other people, such as in the health area, skills are required in addition to the technical functions of professionals. Especially in Primary Health Care, where interpersonal relationships are more intense, manifestations such as emotional exhaustion, anxiety, and depersonalization suggest that the worker is suffering from Burnout Syndrome. Seeking to relieve these symptoms, the professional uses psychotropic drugs, which act directly on the central nervous system, changing emotion, mood and behavior. The objective of this study was to evaluate the incidence of Burnout Syndrome and the use of psychotropic drugs in workers at Primary Health Care units in the city of Alfenas-MG. Materials and methods: This is a descriptive, quantitative and cross-sectional study, in which data were collected from March to June 2017 in the basic health units, through the application of two questionnaires: the Jbeili questionnaire for the preliminary identification of Burnout, and another questionnaire prepared by the researcher to evaluate the use of psychotropic drugs. Results and conclusion: A total of 219 professionals participated in the study, with a mean age of 40.4 years, most of them female (80.8%) and with a time of service in the area of less than 5 years (49.8%). It was observed that Burnout Syndrome is present in its initial stages in 45.2% of the participants and that the most relevant manifestation (37.4%) was depersonalization. The use of psychoactive drugs was reported by 16.9% of workers, and the most commonly used drugs were Fluoxetine and Sertraline. With these data, it can be seen that is necessary to propose strategies to receive these workers and eliminate or attenuate stressors in the work environment.

CHOCOLATE AS AN ALTERNATIVE FORM OF CAFFEINE SUPPLEMENT FOR ATHLETES

CFSP026

ARIANNE KAROLINNE CARVALHO; AMANDA ELLEN COSTA MELLO; LILIAN CRISTINA SILVA TORRES; MARILÚCIA RODRIGUES DE OLIVEIRA; NATÁLIA RAMOS LUIZ; NILDE SANTOS DE MELO; RENATA ANTUNES ESTAIANO DE REZENDE; ERICO CHAGAS CAPERUTO; MARCO AURÉLIO LAMOLHA. Universidade São Judas Tadeu, São Paulo - SP - Brazil. Introduction and objective: Caffeine has been widely used by athletes as an ergogenic resource, in addition to increasing processing speed and coding new information. However, the biggest challenge in developing formulations of caffeine for oral use is to mask its bitter taste. The present work aimed to develop and evaluate alternative formulations of caffeine supplementation for athletes in the form of chocolate lozenges in order to offer a preparation with a pleasant flavor and to improve compliance. Materials and methods: Two types of chocolate were used in the lozenge manipulation: A-Blend; (SICAO, Brazil) and B-Diet (Nestlé, Brazil), prepared by melting and molding, as indicated by the manufacturers. The following parameters were evaluated: average weight (g) content (%) of moisture, ashes, lipids and proteins, according to the methodologies recommended by IAL (2008) and compared, by T statistics, as a function of p values. Results and conclusion: The lozenge developed had the following characteristics: - A: Average weight 20.714g; Humidity 2.06%; Ash content 1.45%; Lipids 37.44%; 4.66% proteins. B: Average weight 16.668g; Humidity 2.11%; Ash content 1.62%; Lipids 0.64%; Proteins 4.79%. Statistical analysis showed that the lozenges presented significant differences for the lipid parameter (p = 4.60x10-8), evidencing this nutritional advantage of the Diet compared with Blend. However, the taste of the Blend was slightly more pleasant. It was possible to develop lozenges with adequate visual appearance, pleasant taste without the perception of the residual bitter caffeine taste, which can become a viable and attractive option as an alternative form for supplementation in athletes.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP027 CLINICAL OUTCOMES FOR TUBERCULOSIS TREATMENT IN HOSPITALIZED PATIENTS AT A UNIVERSITY HOSPITAL IN THE CITY OF BELÉM-PA. FABÍOLLA DE CÁSSIA SOARES CARDOSO; ADRIANA DO SOCORRO UCHOA DA SILVA; PAULA SOUSA DA SILVA ROCHA; MARCIENI ATAIDE DE ANDRADE; MARCOS VALÉRIO DA SILVA. Universidade Federal do Pará, Belém - PA - Brazil. Introduction and objective: Tuberculosis, caused by Mycobacterium tuberculosis, is an infectious-contagious disease that remains an important cause of morbidity and mortality in the world, including all age groups and is exacerbated by precarious socioeconomic conditions. The objective of this study was to evaluate the clinical outcome for the treatment of tuberculosis in a referral hospital located in the city of Belém. Materials and methods: A descriptive, quantitative, cross-sectional and statistical study was conducted, through the collection of data obtained from medical records of inpatients in João de Barros Barreto University Hospital between January 2005 and December 2011. Results and conclusion: A total of 2,915 patients diagnosed with Tuberculosis that were found in the MFSD (Medical File and Statistics Division) database were analyzed, of which two types of hospital discharges were observed in the patients studied during the study period: Improved discharge and death discharge. The study identified that 86% (2500) left with improved hospital discharge and 14% (415) were discharged by death, in this sense it was verified that the hospitalization rate in the studied period presented a significant decreasing trend, evidencing the values of the therapeutic success achieved through strategies implemented that help the patient to overcome the negative aspects of the disease motivating the treatment. Financial support or fellowship: HUJBB - UFPa

CLINICAL PHARMACIST IN ONCOLOGY: EFFECTIVE CONTRIBUTION TO PATIENT SAFETY

CFSP028

CRISTINA ROSA BARBOSA1; LARA PARO DIAS1; MARIANE GALVÃO ROBERTO TAVARES1; NATALIA DA COSTA DUARTE2; PATRICIA MORIEL2. Outpatient Clinic of Oncology and Chemotherapy/Hospital de Clínicas, Unicamp, Brazil, Campinas - SP - Brazil; 2Faculty of Pharmaceutical Sciences, Unicamp, Brazil, Campinas - SP - Brazil. 1

Introduction and objective: Medication errors are important causes of morbidity and mortality in health care systems. Several authors cite the contribution of the clinical pharmacist in the identification and prevention of these errors, by performing pharmaceutical intervention in the process of medication use. The aim of this study was to characterize and identify errors of medication and pharmaceutical interventions (PI) in the Outpatient Clinic of Oncology and Chemotherapy at the Hospital de Clínicas/ UNICAMP and to propose improvements in the process, considering the identified medication errors. Materials and methods: This is a prospective, quantitative study with a duration of six months (from December 2016 to May 2017). Necessary PI were identified in the evaluation process of the prescription. PI were characterized regarding the process step in which they were held and the acceptability by the multi-professional team. Sample at an estimated error of 1.0% (5% alpha). Results and conclusion: The number of patients treated by the chemotherapy pharmacy generated 3,526 prescriptions (one prescription per patient). Three hundred and forty-one (341) interventions were carried out (9.67%) in the prescription of 221 patients (average age 60.67 ± 13.23 years old). More than one error was found per prescription, and the most frequent mistakes were found in Ondasentron (13.78%) and Carboplatin (15.24%) drugs. The main mistake was dosage (40.76%) followed by dilution (18.18%). Only two interventions were not accepted (0.58%). The clinical pharmacist’s role in the analysis of requirements and their PI effectively contributes to patient safety.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP029 CLINICAL PHARMACIST INTERVENTIONS PROMOTING RATIONAL USE OF ANTIMICROBIALS IN ADULT INTENSIVE CARE UNITS OF A PRIVATE HOSPITAL JAQUELINE PILON DE MENESES; JULIANA FERNANDES ROMERA; JULIANA RIBEIRO DE ASSIS; RENATA DANTAS CORREIA; CARLA CAROLINE SILVA; ANA BEATRIZ DO VALLE FERNANDES; VANESSA DE ANDRADE CONCEIÇÃO; VINICIUS CESAR DA SILVA MOREIRA. Hospital Santa Catarina, São Paulo - SP - Brazil. Introduction and objective: The dramatic increase in antimicrobial resistance seen in many hospitals combined with the paucity of new drug development calls for prudent and appropriate use of antimicrobials. The clinical pharmacist can contribute to the rational use of antimicrobials as a specialist. Objective: To evaluate antimicrobial-related pharmacist interventions and to analyze medical acceptance of interventions in adult Intensive Care Units (ICUs). Materials and methods: A retrospective cohort study was conducted with the prescriptions in adult ICUs between January, 01, 2016 and December, 31, 2016. It was considered one patient per prescription in antimicrobial-related pharmacist interventions. To determine the taxonomy of interventions, an adapted version of the National Coordinating Council for Medication Error Reporting and Prevention (NCC MERP) was performed. Results and conclusion: 12,604 prescriptions were analyzed. Some 5,469 interventions were carried out and of these, 754 (13.8%) were antimicrobial-related, following taxonomy: Improper dose (36.1%), wrong frequency (10.1%), wrong time (8.6%), wrong duration (8.1%), wrong drug (6.8%), wrong strength/concentration (5.0%), duplicity (4.8%), dose omission (4.1%), wrong count (4.0%), noncompliance in the medication process (3.8%), drug interaction (2.9%), monitoring error (1.9%), wrong pharmaceutical form (1.9%), wrong route of administration (1.2%), drug reconciliation failure (0.4%) and incompatibility (0.3%). A high medical acceptance of the pharmacist interventions (90.8%) was found. The contribution of clinical pharmacists in the ICU is an important measure to reduce irrational use of antimicrobials. CFSP030 COGNITIVE PRESERVATION IN PATIENTS WITH ALZHEIMER’S DISEASE AND USE OF BENZODIAZEPINES MARCELA FORGERINI; FERNANDA MARIANA DE OLIVEIRA; PATRÍCIA DE CARVALHO MASTROIANNI. São Paulo State University (Unesp), Araraquara - SP - Brazil. Introduction and objective: It was proposed to evaluate the effectiveness of anticholinesterase pharmacotherapeutics in patients diagnosed with Alzheimer’s disease (AD) with or without concomitant use of benzodiazepines. Materials and methods: Experimental, longitudinal and non-randomized study, carried out at Centro de Referência do Idoso de Araraquara (CRIA), with 55 patients diagnosed with and in treatment for AD living in the city of Araraquara/SP. The Integral Pharmacotherapy Management (IPM) was conducted according to the Pharmacotherapy Workup (PW), method and complemented by the Medication Experience (ME), which contributed to the evaluation of Drug Therapy Problems (DTP) at baseline and after the intervention. The first visit lasted about an hour and the follow-up, 30 minutes, with an average of six visits per patient. The variables of interest were cognition [measured by the Mini Mental State Examination test (MMSE)], staging of the disease [measured by the Clinical Dementia Rating (CDR)]. The primary outcome is cognitive impairment. The effectiveness of anticholinesterases was evaluated by the Student’s T-Test. The comparison of cognitive impairment among patients who used benzodiazepines and those who did not, was evaluated by the non-parametric Mann-Whitney test. It was approved by CEP according to CAAE: 08172112.0.0000.5426. Results and conclusion: 16 were using benzodiazepines and 39 were not. The IPM proposal evidenced the effectiveness of anticholinesterase pharmacotherapeutics in patients diagnosed with Alzheimer’s disease due to cognitive preservation, according to the MMSE (p 0.23) and CDR (p 0.05) parameters. Concomitant use of benzodiazepines also did not influence cognitive impairment Financial support or fellowship: CAPES, FAPESP, PADC-FCFAR/UNESP, PROEX.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP031 COMPARATIVE ANALYSIS IN THE DISPENSATION OF METHYLPHENIDATE CHLORIDE FROM THE PERIODS OF AUGUST TO OCTOBER OF 2015 AND 2016. CARLA LARISSA DE CASTRO VIEIRA; LUANA DE FÁTIMA FALCÃO LIMA; MARIA DO CARMO DA SILVA ROCHA BESERRA; FABIA MARIA BARROSO DA SILVA LOBÔ; EXPEDITO ROGILDO CORDEIRO CARLOS. Universidade de Fortaleza - Unifor, Fortaleza - CE - Brazil. Introduction and objective: Psychotropic drugs are used for a variety of purposes, including the treatment of mental disorders, since they can act in the central nervous system. However, these drugs can cause dependency, therefore they must be prescribed only in a special control notice as regulated by decree 344/98. Our objective is to undertake a quantitative analysis of the dispensation of methylphenidate chloride in a drug store in the periods from August to October of both 2015 and 2016. Materials and methods: An analysis was performed of the methylphenidate chloride prescriptions at a drugstore in the city of Fortaleza. The prescriptions from the period of August to October 2015 and 2016. Results and conclusion: Of all prescriptions analyzed in the 2015 quarter 81 prescriptions of methylphenidate were obtained. In the 2016 quarter there were 61 prescriptions. Of those prescriptions, 77.5% were for Ritalin® and 22.1 % were Concerta®, in the 2015 quarter. We found 64% of Ritalin® 10mg; 3.7% of Ritalin® 20mg; 8.6% of Ritalin® 30mg; 1.2% of Ritalin® 40mg. Concerta 18mg 2.5%; Concerta® 36mg 8.6%; Concerta® 54mg 11.1%. In the same period of the following year, a total of 86.7% in the dispensation of Ritalin® and 13.1% of Concerta® was obtained. We found 62.2% of Ritalin® 10mg; 1.6% of Ritalin® 20mg; 13.1% of Ritalin® de 30mg; 9.8% of Ritalin® de 40mg. Concerta® 54mg 13.1%. In the face of data presented we may conclude that the use of methylphenidate has been growing gradually in Brazil, nevertheless the concentrations which are prescribed are the lowest.

COMPLIANCE EVALUATION OF RDC 44/09 BY VIRTUAL BRAZILIAN PHARMACIES

CFSP032

ANA PAULA DE ARAUJO ABREU; THAYNARA FERREIRA PINHEIRO; JOSE CHAVES OLIVEIRA; FLAVIO DONALWAN SA MAXIMINO. Faculdade Pitágoras, São Luís - MA - Brazil. Introduction and objective: The medicine sale through online pharmacies represents a trend, but it still suffers little regulation. The RDC nº 44/2009 provides good dispensing practices and pharmaceutical product commercialization, including support in virtual sales. Objectives: Demonstrate compliance with RDC 44/09 of sites that sell drugs online. Materials and methods: A descriptive study. A search for virtual pharmacies was executed using the keyword “online pharmacy” in July 2017, the inclusion criterion are drugs sale websites with final “.br” URLs and the exclusion criterion are websites that do not sell prescription drugs. It was observed whether the websites met the criteria established in RDC 44/09. Results and conclusion: We selected 12 pharmacies that fulfilled the previous criteria, 33.3% (4 sites) did not show the authorization of operation (AFE), 50% (6) did not show operation hours and 8.3% (1) of the pharmacies did not put the alerts and sanitary recommendations determined by ANVISA. Only 42% (5) of the sites had the option of contacting the pharmacist, and only 16.7% (2) provided a 24-hour pharmacist contact form. According to this RDC, the pharmaceutical establishment should assure the user right to information and guidance regarding use of the medicines requested by remote means. During the search it was noticed that 25% (2) of the websites show customers’ evaluations of the products on the main page. This situation, as well as the difficulty of access to the pharmaceutical professional, represents a risk to the patient, and a possibility of self-medication induction. In view of this, it is important to supervise these sites, and to bring to public a discussion about the importance of increasing access to this professional throughout the hours of operation (sales) of the site.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP033 CONSTRUCTION OF THE PROFILE OF USERS WITH SCHIZOPHRENIA THROUGH THE DISPENSATION OF ANTIPSYCHOTICS BY THE SPECIALIZED COMPONENT PHARMACY IN SOBRAL-CE ANTONIA RAFAELLA FERREIRA GOMES MARTINS1; JOÃO PAULO DE MEDEIROS JÚNIOR2; BRENA GEYSE MESQUITA ROCHA SOARES3; ADNA VASCONCELOS FONTELES3; EMANUELLA MACEDO SILVA3; OLINDINA FERREIRA MELO DE CHAVES2. Santa Casa de Misericórdia de Sobral -CE, Sobral - Brazil; 2Instituto Superior de Teologia Aplicada, Sobral - CE - Brazil; 3Santa Casa de Misericórdia de Sobral-CE, Sobral - CE - Brazil. 1

Introduction and objective: Schizophrenia is the most common severe psychiatric disorder, affecting approximately 20 million people worldwide and causing significant cognitive, functional, and affective impacts. To construct the profile of schizophrenic patients through the analysis of the consumption of antipsychotics in the Specialized Component Pharmacy in Sobral-CE. Materials and methods: This is an exploratory, descriptive, quantitative study. The research period was from August to November 2015 in the Pharmacy of the Specialized Component of the Municipality of Sobral-CE. Data collection was done through the analysis of 147 Exceptional Drug Request Reports (LME) of all patients who were diagnosed with schizophrenia and the data analysis was conducted using a form that consisted of the patient’s diagnosis, age, sex, ethnicity, prescribed antipsychotic, and accessibility to the drug. The study was approved by the Nucleus of Education and Research of Sobral (NEPS) with CAAE No.: 47349715.5.0000.5053, being a part of the research “Profile of the users of antipsychotics in the Pharmacy of the Specialized Component in Sobral-CE”. Results and conclusion: The results obtained from the analysis of the 147 LMEs were: mean age 41-50 years (19.72%), mostly male (52%), and ethnicity brown (75%). The most commonly prescribed antipsychotic was Risperidone (32% of patients), which was difficult to access for 69% of those patients. The results followed the literary standard, i.e., the main therapeutic choice (Risperidone) for the treatment of users with schizophrenia followed the Clinical Protocols and Therapeutic Guidelines of Schizophrenia, but the regularity of receiving the medication was flawed due to a possible error in the logistics of the central pharmacy, which is located in Fortaleza, that dispenses the medicines to the municipality of Sobral; this is not necessarily the responsibility of its manager, but simply portrays the financial and political crisis that affects the population, who are entitled by law to receive their corresponding drug. Financial support or fellowship: Santa Casa de Misericórdia de Sobral-CE/Instituto Superior de Teologia Aplicada CFSP034 CONTROL OF THE USE OF ANTIMICROBIAL AGENTS IN THE CURRENT CONTEXT IN THE MUNICIPALITY OF ITAPIRA, STATE OF SÃO PAULO, BRAZIL LARISSA APARECIDA DOS SANTOS; RENATA LOPES RODRIGUES; NÁDIA REGINA BURIM ZUIM; CAMILA STÉFANI ESTANCIAL FERNANDES; DANYELLE CRISTINE MARINI. Fimi, Mogi Guaçu - SP - Brazil. Introduction and objective: Antimicrobial agents are among the most prescribed classes of medication, but are often used incorrectly. The indiscriminate use of these drugs is directly related to the development of resistant microorganisms. Thus, rational use is required, with the correct indication considering the clinical situation, the results of microbiological tests, and the adequate adherence to treatment. Problems stemming from indiscriminate use motivated the Brazilian National Sanitary Surveillance Agency to issue Resolution No. 20/2011, which restricts the use of antimicrobial agents. As a result, prescriptions are currently valid for only ten days and dispensing of the drugs requires the retention of the prescription at the pharmacy. These aspects are controlled by the National Controlled Product Management System. The aim of the present study was to evaluate implications regarding the acquisition of antimicrobial agents on the part of society after the publication of the resolution. Materials and methods: A cross-sectional study was conducted in neighborhoods in the municipality of Itapira in the state of São Paulo, Brazil, following approval from the local ethics committee (certificate number: 55192816.2.0000.5679). The participants were selected following the determination of residences using probabilistic sampling. A questionnaire addressing the use and acquisition of antimicrobial agents was administered. Results and conclusion: One hundred and fifty individuals were interviewed, 97% of whom made use of antimicrobial agents. The findings demonstrate that the majority of the population acquired such medications through prescriptions (65%), but had inadequate knowledge with regard to the established requirements and the importance of the proper use.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP035 CONVERSATION CIRCLES WITH PHARMACISTS WHO WORK IN SUS – THE EXPERIENCE OF THE PUBLIC PHARMACEUTICAL ASSISTANCE ADVISORY COMMISSION OF THE CRF/SC FERNANDA MANZINI1; CASSIA CUNICO2; CRISTIANA ROPELATTO CAETANO3; HELOÁ KLABUNDE4; JANAINA PRAVATO VICENTE BANIN5; LIGIA HOEPNER6; MAIKE LIA FADL DE KRAUSSER7; PHEANDRO AUGUSTO ROSSI BARRETO8; SILVANA NAIR LEITE1. Ufsc, Florianópolis - SC - Brazil; 21ª Gerência de Saúde de São Miguel do Oeste, São Miguel do Oeste - SC - Brazil; 3Secretaria de Saúde de Rio do Sul, Rio do Sul - SC - Brazil; 4Prefeitura de Guabiruba, Guabiruba - SC - Brazil; 5Secretaria Municipal de Joinville, Joinville - SC - Brazil; 6Prefeitura de Pomerode, Pomerode - SC - Brazil; 7Secretaria de Saúde de Blumenau, Blumenau - SC - Brazil; 8 Secretaria de Saúde de Itajaí, Itajaí - SC - Brazil. 1

Introduction and objective: Introduction: The Public Pharmaceutical Assistance Advisory Commission (COMSUS) of the CRF/ SC aims to promote and develop actions with the purpose of enhancing public health and adding value to the pharmacist in pharmaceutical assistance (PA) within the SUS. Aiming to approach the pharmacists, conversation circles were conducted in several cities of the state. “Conversation circles” was the strategy adopted to allow wide participation, understanding that all participants are carriers of knowledge and can contribute to the resolution of the reported doubts or problems. Conversation circles are an effective tool for establishing dialog and interaction (Moura, Lima, 2014). The work aims to report the experience of promoting conversation circles in Santa Catarina. Materials and methods: To organize the topics to be addressed, the pharmacists of the Section responded to an on-line questionnaire in which they indicated subjects of interest. Verification sheets for the professional ethical practice in the public pharmacy were also analyzed. Audios of the discussions were recorded and minutes were taken. Results and conclusion: From 2014 to 2017, 13 conversation circles were held, with 400 participants (pharmacists, students, health secretaries and other professionals). They were conducted in order to allow all participants to express themselves, and to present experiences in relation to the problems reported. Even with managerial and structural problems, the public PA has made progress, with increased hiring of pharmacists, clinical activities performed and new forms of organization such as Technical Chambers and Consortia. A network of contacts between pharmacists of the various sections was established, which promotes the continuity of discussions. The experience obtained through the conversation circles achieved the expected goals. They were privileged spaces for the debate, and allowed the approximation with the different realities and practices of the pharmacists who work in SUS. It is with the experience and discussion of the results of the conversation circles that COMSUS is planning its actions, aiming at strengthening the public PA in Santa Catarina. CFSP036 CRITICAL ANALYSIS OF THE REUSE OF UNUSED MEDICINES AS A STRATEGY TO REDUCE INADEQUATE DISPOSAL MARIANA DA MATA ALVES; THAIS ROBERTA DA SILVA; VALTER LUIS DA COSTA JUNIOR; LUCIANE MARIA RIBEIRO NETO. Centro Universitário São Camilo, São Paulo - SP - Brazil. Introduction and objective: As a result of the overuse of drugs, many of these products end up being disposed of inappropriately and could lead to contamination of the environment. In order to solve this question, a change in social behavior is required, especially in the production and consumption patterns aiming at the decrease of unused medicines to be discarded. Thus, the aim was to integrate the University Pharmacy with the project of University Extension 5Rs of a Higher Education Institution - São Paulo. To assist in the analysis of the possibility of reusing discarded drugs within the validity date with a view to the Rational Use of Medication (URM) and the preservation of the environment. Materials and methods: The collection of the material was coordinated by the 5Rs Program. The medicaments discarded by people from both University São Camilo-SP campuses were evaluated qualitatively and quantitatively from September -2013 to February2015. The expiration date of each unit of discarded human use was compiled. Discarded drugs were ordered in two groups according to the expiration date, one being dated before September -2013 and the other with a date after September -2013. These data were analyzed for the amount discarded, pharmacological groups of the medicines and expiration date. Results and conclusion: Discarded synthetic human allopathic drugs with a shelf life greater than September - 2013 corresponded to 52.82%. Of these 10.59% were analgesics, followed by anti-inflammatories (8.88%), antibiotics (7.52%) and antiemetics (2.05%). The other pharmacological classes accounted for 70.96%. It was observed that of discarded drugs with a shelf life beyond September -2013, 18.88% had a shelf life beyond February – 2015. Due to the significant number of drug units discarded within the period of validity by the population that attends Campus University São Camilo- SP, it is necessary to improve the specific collection procedures for these unused medicines in order to trace their origin and conditions The transfer of medicines to the population from donations may be practicable if therapeutic efficacy is assured based on the provenance of the medicines.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP037 DEMOGRAPHIC AND CLINICAL CHARACTERIZATION OF PATIENTS WITH MELANOMA TREATED WITH ANTI-BRAF THERAPY IN THE HOSPITAL OF CANCRO DE BARRETOS BETWEEN 2012 AND 2015. TATIANA MAIA DA SILVA1; LETICIA MARIA MODES DA COSTA2; CAMILA SOUZA CROVADOR3; VINICIUS DE LIMA VAZQUEZ4. Unifeb, Barretos - SP - Brazil; 2Facisb, Guaira - SP - Brazil; 3Fundação Pio Xii, São José do Rio Preto - SP - Brazil; 4Fundação Pio Xii, Barretos - SP - Brazil. 1

Introduction and objective: Melanoma is the most lethal type of skin cancer. The incidence has increased in recent years among young adults. As well as the development of target-drugs in the era of personalized medicine. The aim of this research was to characterize the melanoma disease in its demographic, histopathological and molecular aspects in patients who underwent antiBRAF treatment. Materials and methods: Retrospective cohort study with the data collected from medical records of the Barretos Cancer Hospital, classified according to treatment, demographic, histopathological and molecular characteristics between the years 2012 and 2015. Results and conclusion: Data from 509 patients were analyzed. Of these, only 8 used anti-BRAF specific-drug, 5 of whom were male; White skin corresponded to 32.5% (phototype I and II); and 37.5% with chronic sun exposure. All residents of urban areas. The anatomical location of the primary tumor prevailed in the trunk (50% of cases), followed by lower limbs (25% cases). As for the histological subtype, there is a rare case of mucosa, but most cases (1/4) were nodular. In 75% of cases, the initial advanced staging (III and IV) prevailed. There were 2 cases that underwent treatment with ipilimumab, one of which was initially treated with interferon alpha. The BRAF mutation analysis was performed in 5 of the cases that had sufficient and quality material for anti-BRAF analysis, 3 of which had positive mutation results. These positive cases got treatment after judicial intervention: 1 case was treated with interferon alpha, followed by vemurafenib. Another case was treated with vemurafenib and finally, another case was treated with traditional chemo following vemurafenib. Currently, of these 8 cases, 3 are being followed up at the institution. Target-treatment is an innovative and recent treatment. Unfortunately, difficult access to drugs hinders the possibility of fast and effective treatment. This impacts treatment and survival, due to the high mortality of melanoma. Financial support or fellowship: CLMM student research Fellowship grant 2015/19969-7 from FAPESP - São Paulo State Research Foundation - São Paulo Brazil CFSP038 DETERMINATION OF THE GLYCAEMIC LEVEL OF RESIDENTS OF A RURAL AREA OF ARAÇATUBA/SP MAYSA ZUCON DA SILVA; MARIANA EVA SOBRINHO; THAYNARA F RUANO DE OLIVEIRA; MILENA DEOLINDO VIEIRA. Universidade Paulista, Araçatuba - SP - Brazil. Introduction and objective: Diabetes Mellitus (DM), a chronic and often asymptomatic disease, is commonly diagnosed in the population. DM is characterized by high levels of glucose in blood for various reasons, such as lack of insulin or insulin resistance, and can be distinguished among different types. In the rural zone, geographic barriers are one of the reasons that prevent residents obtaining health service support. Objectives: Evaluation of glycemic levels in adults living in a rural area of Araçatuba/SP. Materials and methods: A population-based cross-sectional study with a sample of 40 individuals representative of the adult population (20-90 years) of a rural district of Araçatuba/SP. A manual blood glucose meter was used to evaluate glycemic levels in the study population. The data were compiled, analyzed and classified according to reference values. Results and conclusion: 32.5% of the studied population presented normal glycemia; 40% a decreased glucose tolerance and 27.5% Diabetes Mellitus, according to the classification used. Among the 27.5% who presented DM: 54.5% were male and 45.5% female. Glycemia should be regularly monitored in the population. However, it still presents a challenge to Brazilian public health, considering that a satisfactory frequency has not yet been reached. In addition to biological factors, it is important to highlight the influence of environmental factors. Studies such as this should continue to be developed in order to alert the population to the importance of regular glycemic evaluation and preventive measures, in addition to the actual control and treatment of Diabetes Mellitus. Financial support or fellowship: UNIP

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP039 DEVELOPMENT AND EVALUATION OF GUMMY TABLETS WITH LEUCINE FOR SUPPLEMENTATION FOR ATHLETES ALINE DA SILVA SOUZA; ELLEN DE AVILA RAMOS; JESSICA RODRIGUES DE SOUZA; GABRIELLA PONTES NOGUEIRA; MARCO AURÉLIO LAMOLHA. Universidade São Judas Tadeu, São Paulo - SP - Brazil. Introduction and objective: Leucine is a branched-chain amino acid essential to the human diet, which has anabolic effects, acting directly on the preservation of skeletal muscle, by inhibiting muscle proteolysis. It is commonly found only in combinations of amino acids (ex.: BCAA) and generally used in the form of “powder”, presenting unpleasant organoleptic characteristics. Objectives: to develop and evaluate an alternative form of presentation of leucine, in the form of gummy tablets, in order to facilitate the use and improve the sensorial aspect of the supplement. Materials and methods: 30 tablets containing 1.0 g of leucine were prepared, using glycerinated gelatin “GB” and Goma Fácil® (Embrafarma, Brazil) “GF”, in the flavors: mint, raspberry, cherry and tutti-frutti. The following parameters were evaluated: average weight (g), moisture content% (oven at 105ºC for 3 hours), hardness in Newton (N) and Shore-A (HA) scale and compared using the T statistic, based on the values of p. Results and conclusion: GB: average weight 5.150g; moisture content 10%, hardness 38.9N and 9.45HA. GF: Average weight 4.711g; moisture content 23%; hardness 29.6N and 10,12HA. Statistical analysis showed that the tablets obtained with the evaluated bases present significant differences for these parameters: moisture content (p = 2.59x10-5), hardness in N (p = 0.04) and HA scale (p = 0.01). Therefore, the tablets obtained with GB presented higher resistance to crushing, characterized by higher values of hardness (N) and lower resistance to penetration, due to the lower values of HA hardness, that is, greater flexibility. It was possible to develop tablets with different flavors, with a very pleasant visual appearance, which will certainly facilitate the use of leucine as supplementation for athletes. Financial support or fellowship: Universidade São Judas Tadeu, SÃO PAULO, SP, BRAZIL.

DISPOSAL OF MEDICINES CARRIED OUT BY THE POPULATION OF SANTA HELENA - SC

CFSP040

RÉGIS CARLOS BENVENUTTI1; DENISE ANGELA RIGON2; EVERTON BOFF1; VANESSA DA SILVA CORRALO3. Unoesc e Unochapecó, São Miguel do Oeste e Chapecó - SC - Brazil; 2Unoesc, São Miguel do Oeste - SC - Brazil; 3Unochapecó, Chapecó - SC - Brazil. 1

Introduction and objective: Disposal of unused drugs is a public health concern, since they are substances that can cause toxicity to living beings. These substances cause environmental contamination and cannot be disposed of as common waste. The objective of this study was to investigate how the population of Santa Helena SC discards unused or out-of-date drugs. Materials and methods: We evaluated the knowledge of 100 interviewees about the incorrect disposal of medicines and its consequences, through a questionnaire of exploratory nature and quantitative approach. The study was approved by the Ethics Committee certificate No. 1966136. Results and conclusion: The most frequent destination of medication disposal was the common waste. There was also a report on the use of out-of-date medication, and delivery to the Basic Health Unit was mentioned. The research showed lack of awareness on the part of the population, since less than half of the interviewees knew some kind of consequence of undue discarding. The lack of orientation, accountability and public policies focused on the theme were emphasized.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP041 DISTRIBUTION OF THE TIME DEDICATED TO DIFFERENT PHARMACEUTICAL ACTIVITIES IN HEALTH UNITS IN THE CITY OF SÃO PAULO MARIA GABRIELA BORRACHA; SILVIA STORPIRTIS. FCF-USP, São Paulo - SP - Brazil. Introduction and objective: To quantify the time spent on the different activities performed by pharmacists in the Primary Health Care Centers of the city of Sao Paulo. Materials and methods: the dimensioning was performed by the analysis of the responses of 45 pharmacists who work in health centers in the city of São Paulo to the online questionnaire. The activities performed by the pharmacists in the health units were divided into three groups: clinical (AC); administrative activities (AG: drug management and administrative activities); and technical-cognitive (ATP: educational groups, commissions, pharmaceutical guidelines, health education). Results and conclusion: The majority (89%) of the pharmacists interviewed were women, with an average age of 36 years, on average with 11 years of training and 6 years working in health units; 77% have post graduation; 29% work in more than one health center and 91% work 40 hours a week; 84% of the units were administered by OSS. The pharmacists who answered the questionnaire worked in different types of centers: AMA 24 hours (1), AMA/AMA E/Hora Certa (1), Hora Certa (1), AMA E (4), AMA E/UBS (1), AMA/UBS Integral (8) and UBS (29). The AMA/AMA E/Hora Certa, AMA 24 Hour and Hora Certa units are the units where the pharmacist devotes the majority of time (78%, 61% and 54%, respectively) to AG, with the remainder of the time spent on ATP. Only the Hora Certa performed AC (4%). AMA E/UBS and AMA E presented the highest percentage of time devoted to ATP (68% and 63% respectively), followed by management activities (32% and 35% respectively), only AMA E performed AC (2%). Pharmacists working in AMA/UBS and UBS units had similar activities in which about half the time of the pharmacist is devoted to ATP, followed by AG (40%) and AC (10%). Conclusions: the distribution of time devoted to the different activities of the pharmacists varied with the type of center in which they work; ACs are still the least accomplished activities and for this to gain space it is necessary to reevaluate the workflows from the perspective of shifting the centrality of the pharmacist’s actions from the drug to the user. CFSP042 DRUG INCOMPATIBILITIES: STUDY OF OCCURRENCE AND PROFILE IN A PEDIATRIC ICU OF A UNIVERSITY HOSPITAL FERNANDA BARRETO DA SILVA; LUNA MAYRA DA SILVA E SILVA; DANIELLE FRANÇA FURTADO; IARA ANTONIA LUSTOSA NOGUEIRA; LEANDRA MARLA AIRES TRAVASSOS VIANA. Universidade Federal do Maranhão, São Luís - MA - Brazil. Introduction and objective: Intensive care patients have complex characteristics in their pharmacotherapy, and because of this, they are at increased risk of adverse reactions, interactions and incompatibilities. Objective: To quantify the occurrence of incompatibilities between intravenous drugs and to trace the profile of the most frequent incompatibilities in the Intensive Care Unit (ICU) under study. Materials and methods: Retrospective exploratory study, with descriptive and quantitative analysis of daily records of pharmaceutical interventions, between July and December 2016. Results and conclusion: We analyzed the medical prescriptions of 113 patients, and detected 501 incompatibilities, present in prescriptions of 62 (54.86%) patients. They were: midazolam x omeprazole (n = 32, 6.39%), midazolam x dexamethasone (n = 27, 5.39%) and vancomycin x omeprazole (n = 25, 4.99%). The three most prevalent drug classes were: antimicrobials (n = 201; 40%), with vancomycin (n = 56; 28%); ?(n = 172, 34%) and midazolam (n = 132, 77%) and anticonvulsants (n = 154, 31%), with phenytoin (n = 114, 74%). They are often prescribed in an ICU and stand out in the profile of incompatibilities traced, demonstrating the importance of spreading information regarding the subject studied. The pharmaceutical evaluation of the medical prescription constitutes an important tool for the accomplishment of the pharmaceutical care, contributing to the multiprofessional team and composing an assistance of greater quality and safety for the patient.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

DRUG INTERACTION EVALUATION IN AFFECTIVE DISORDERS TREATMENT: A CASE STUDY

CFSP043

LARISSA FERNANDES 1; MARIA APARECIDA DE BONA DA SILVA 2; ALEXANDRE PICCININI 1; CARLOS HENRIQUE BLUM DA SILVA1; ANDRESSA CORNEO GAZOLA3. Centro Universitário Barriga Verde - Unibave, Orleans - SC - Brazil; 2Centro Universitário Barriga Verde - Unibave, Braço do Norte - SC - Brazil; 3Centro Universitário Barriga Verde - Unibave, Criciuma - SC - Brazil. 1

Introduction and objective: The increasing number of psychiatric diseases has been worrying experts. The lifestyle adopted by the population contributes greatly. The feeling of exhaustion persisting for at least two weeks leads people to lose energy, interest or pleasure, inducing their mood depressed, causing thoughts of guilt, worthlessness and death, characterizing a Depressive Episode. These symptoms vary in each person and, if not treated, can progress to a psychiatric disorder. Generally, psychiatric conditions require more personalized treatments, increasing the complexity of the drugs taken. The administration of several drugs simultaneously is a common and almost always necessary practice, however, it increases the possibility of the occurrence of drug interactions that may influence therapeutic efficacy or accentuate the side effects. In view of this, the present study aimed to analyze drug interactions in the treatment of a patient from the southern region of Santa Catarina State - Brazil with initial diagnosis of severe depressive episode without psychotic symptoms. Materials and methods: A qualitative and quantitative analysis of the data present in the clinical record of the patient was carried out, aiming to identify drug interactions since the patient used 08 different drugs, simultaneously. The Research was approved by the Research Ethics Committee Involving Human Beings Certificate No. 1,611,991. Ethical principles were respected in accordance with Resolution No. 466/2012 of the National Health Council. Results and conclusion: It was identified that the drugs administered from the first to the last diagnosis (bipolar disorder) of the patient constituted the recommended therapy. Based on the drug interactions, it was observed that none of the interactions that occurred could alter the therapeutic efficacy of the treatment, but some of them can increase toxicity of the drugs or the risk of bleeding.

DRUG INTERACTIONS IN PEDIATRIC ICU

CFSP044

DANIELLE FRANÇA FURTADO; LUNA MAYRA DA SILVA E SILVA; PATRICIA BATISTA RIBEIRO CORRÊA; FERNANDA BARRETO DA SILVA; LEANDRA MARLA AIRES TRAVASSOS VIANA; IARA ANTONIA LUSTOSA NOGUEIRA. Hospital Universitário da Universidade Federal do Maranhão, São Luís - MA - Brazil. Introduction and objective: Concomitant prescription of drugs with potentially serious interactions in pediatric patients may contribute to morbidity and increased treatment costs. Children exhibit pharmacokinetic and pharmacodynamic particulars that change with development, making them vulnerable to the occurrence of adverse events. This work aims to describe the drug interactions identified by pharmacotherapeutic monitoring in prescriptions of pediatric patients Materials and methods: This is a descriptive, retrospective study with a quantitative approach, performed by the Clinical Pharmacy service in the Pediatric ICU of the University Hospital of the Federal University of Maranhão-HUUFMA from September 2016 to April 2017. Analysis of the occurrence of Drug Interactions is conducted daily by means of an evaluation of the pharmacotherapy in the medical prescription, by consulting the medical record and consulting the MICROMEDEX 2.0 database to define the potential interactions. They are then recorded in specific worksheets and reports for communication and discussion with the medical staff. The drug interactions were quantified by consulting the records of the Clinical Pharmacy service and classified for severity as follows: Contraindicated, when the drugs are contraindicated for concomitant use; Important, when they cause serious adverse effects; Moderate, when it results in exacerbation of the health problem. Results and conclusion: 112 patient charts were analyzed, of which 53 (47.3%) had at least one drug interaction. The total drug interactions were 201. In relation to severity, 5 (2.5%) were contraindicated, 105 (52.2%) were significant and 91 (45.3%) were moderate. Thus, the analysis of drug interactions subsidizes the adequate management of the therapy, allowing individualized assessment of risk and benefit, with the performance of the clinical pharmacist in the ICU standing out as contributing to the prevention of adverse events.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

DRUG INTERACTIONS INVOLVING HIGH-ALERT MEDICATIONS IN A GENERAL HOSPITAL

CFSP045

BEATRIZ LEAL ELIAS1; FÁTIMA CRISTIANE LOPES GOULARTE FARHAT2. Universidade Metodista de Piracicaba/Unimep, Piracicaba - Brazil; 2Universidade Metodista de Piracicaba/Unimep, Piracicaba - SP - Brazil. 1

Introduction and objective: The patient’s safety in the health system is discussed worldwide, showing the necessity of information and constant reorganization of actions and service process. In this context, the safe administration of medicines assumes an important role, it also involves prevention and monitoring of unwanted drug interactions. Anticoagulant, hypoglycemic, vasoactive, among other drugs, have appeared in the lists of medications named as high-alert medications (HAM) in the hospital service and the interaction involving these medicines can represent a high risk of significant damage to the patients. This study aimed to identify the prevalence of important clinical drug interactions involving HAM in a large general hospital in São Paulo state. Materials and methods: Cross-sectional, descriptive study was carried out with the identification of a HAM list at the hospital, bibliographic search and at MICROMEDEX® about their drug interaction, followed by prescription analyses from their hospitalization unit, once a week for 10 weeks. Approved by CEP/Unimep (nº 60/2016). Results and conclusion: Some 2340 prescriptions were analyzed, of which 642(27.4%) were with HAM and of these, 244 clinically relevant interactions occurred (an average of 2.6 prescriptions with HAM). The most prevalent involved midazolam (125;51.2% of interactions) and heparin (92;37.7% of interactions). Most of the interactions found, advised either clinical monitoring or laboratory of the patient, at risk of aggravate their health. It was verified that there was little information in the literature about hospitalization involving HAM, which can make either the prescription or the usage vulnerable and unsafe. The results of this study will subsidize the pharmaceutic work in a multiprofessional team, besides the electronic prescription system of the institution, thus contributing to patient safety. Financial support or fellowship: FAPIC/UNIMEP

DRUG THERAPY FOLLOW UP IN ELDERLY PATIENTS

CFSP046

CARLA MARIA BERNARDES SCANAVACHI1; DANYELLE CRISTINE MARINI2. Unifae, Santo Antônio do Jardim - SP - Brazil; 2Unifae, Mogi Guaçu - SP - Brazil.

Introduction and objective: Drug therapy follow up is a pharmaceutical care tool, the aim of which is to prevent and attempt to solve medication-related problems, thereby improving both the therapy and quality of life of patients. The aim of the present study was to perform drug therapy follow up of patients at a geriatric home, verifying the drug therapies used as well as evaluating drug interactions and the negative impact such interactions can have on patient health. Materials and methods: A descriptive, cross-sectional study was conducted following approval from the human research ethics committee of UNIFAE (certificate No. 67388417.6.0000.5382). Ten patients were evaluated with the aid of a care plan employing the SOAP note documentation method and the evaluation of patient records. Socioeconomic factors, the drug therapy of each patient and medication-related problems were investigated. Results and conclusion: Three women and seven men participated in the study. Severe, moderate and mild medication-related problems were found following an analysis of the information on medications and symptoms presented by the patients. The negative results underscore the need for individualized care on the part of the multidisciplinary team. For cases in which it was necessary to alter the therapy, a letter was sent to the prescribing physician requesting a change to the prescription. Ways to monitor the results of the plan implemented were also established, such as verifying whether changes occurred in the prescription or condition of the patient, thereby introducing drug therapy follow up.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP047 DRUG THERAPY FOLLOW UP OF ELDERLY PATIENTS IN A HOME IN SÃO JOÃO DA BOA VISTA, STATE OF SÃO PAULO, BRAZIL ROBSON GONÇALVES DE ANDRADE1; DANYELLE CRISTINE MARINI2. Unifae, São João da Boa Vista - SP - Brazil; 2Unifae, Mogi Guaçu - SP - Brazil.

Introduction and objective: Drug therapy follow up is an important tool for reducing medication errors, which can affect the efficacy of treatment, and achieving improvements in both patient health and quality of life. For elderly individuals, quality of life depends on both emotional and physical health. With the advance in age, functional capacity diminishes, accompanied by a tendency toward the loss of autonomy. Moreover, ageing is often accompanied by chronic diseases and a consequent increase in the use of medications. The aim of the present study was to perform drug therapy follow up of elderly residents aged 60 years or older at a geriatric home located in the municipality of São João da Boa Vista, state of São Paulo, Brazil. Materials and methods: A cross-sectional study was conducted following approval from the local human research ethics committee (certificate No. 67388317.5.0000.5382). Ten patients were evaluated with the aid of a care plan employing the SOAP note documentation method, which enables the collection and organization of patient data in order to identify medication-related problems and perform drug therapy follow up. Results and conclusion: Ten residents of a geriatric home participated in the study. Several severe, moderate and mild drug interactions were identified. Based on the findings, a conduct plan was determined and, when necessary, a letter was sent to the prescribing physician requesting a review of the medications. Another plan was established for patient monitoring with the involvement of the multidisciplinary team to ensure drug therapy follow up. CFSP048 DRUG TREATMENT ADHESION IN PATIENTS WITH CORONARY ARTERY DISEASE: MULTICENTER STUDY ANNA KARLA CÉSAR LEANDRO1; FRANCISCO DE ASSIS COSTA2; ROBERT ANDERSSON FIRMIANO NICÁCIO3; ALFREDO DIAS DE OLIVEIRA FILHO2. Ibras, Maceió - AL - Brazil; 2Ufal, Maceió - AL - Brazil; 3CRF/Al, Maceió - AL - Brazil.

Introduction and objective: Medication adherence is a complex behavioral outcome, heavily influenced by socioeconomic factors and health condition. Coronary Artery Disease (CAD) is the most common cause of death globally, and it is strongly influenced by drug treatment, which in turn can be decisively affected by inappropriate medication adherence. The objective was to examine the prevalence of medication adherence in patients with CAD. Materials and methods: A cross-sectional multicentric study was carried out with patients aged over 18 years, with angiographic evidence of CAD in Maceio, Brazil, between October 2016 and March 2017. Results and conclusion: 118 patients were included, with mean age of 65.1 years and average body mass index equal to 27.6. Most patients (61%) were male. Approximately 61% of patients had low schooling: 10.2% of patients were illiterate and 51% had incomplete elementary school. Only 53 (44.9%) patients adhered to prescribed therapy. The average use of medicines was 4.9 per patient. Although increased medication adherence has been associated with improved CAD outcomes and reduced costs, patients showed poor adherent behavior.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

WEIGHT LOSS DRUG USE AMONG STUDENTS AT A UNIVERSITY CENTER

CFSP049

WYLGNER BAGIO MACIESKI; ROSANGELA MORGAN SCHEPER; ANA PAULA BAZO; LEONARDO DE PAULA MARTINS; CLÁUDIO SÉRGIO DA COSTA. Centro Universitário Barriga Verde - Unibave, Orleans - SC - Brazil. Introduction and objective: Nowadays, there are many drugs for the pharmacological treatment of obesity and according to the International Narcotics Control Board, Brazil is the country with the highest consumption of medications for weight loss, which is worrying, since these can generate dependencies, besides other side effects. In this context, the present study aimed to identify the consumption prevalence of medications for weight loss among university students. Materials and methods: Data was collected by a questionnaire, sent by e-mail to all 2,158 students regularly enrolled at the beginning of the second half of 2016 in a university center in the south of Santa Catarina State. In total, 396 students answered the questionnaire. All participants received, attached to the questionnaire, the Informed Consent Form, In addition, the present study was evaluated and approved by the Research Ethics Committee, certificate No. 1,611,900. Results and conclusion: Of the participants, 112 (28%) had already made use of some substance for weight loss, of whom most (85.7%) were female. The most commonly used drugs were: green tea, sibutramine, Herbalife® and fluoxetine. One of the main reasons for the use of these diet pills was body esthetics. However, when observing the Body Mass Index of these participants, it is found that 53.2% were within the normal values. When it was questioned about indication for use, 60.7% answered that it had been by own decision and 11.6% from the suggestion of friends, relatives and neighbors. Prescriptions of the medicine by health professionals (physician, nutritionist and/or pharmacist) was cited by only 30.4% of the research participants. Among the adverse reactions indicated by 36.9% of the participants, the most cited were insomnia, irritability and headache. In this sense, measures can be offered by pharmacists at the time of dispensing, guiding patients about rational use of medicines and possible adverse reactions. CFSP050 EFFECTS OF ACUPUNCTURE AND AURICULOTHERAPY ON PAIN AND INFLAMMATION OF PATIENTS WITH LOWER BACK PAIN JOSÉ TREZZA NETTO; ELEINE WEIMANN; BEATRIZ BELMIRO DIAS; ELAINE HATANAKA. Universidade Cruzeiro do Sul- Unicsul, São Paulo - SP - Brazil. Introduction and objective: Lower back pain is a neuralgia usually caused by nerve root compression and/or inflammation. The pain is often associated with tingling, numbness and weakness of the leg; it may be sudden in onset and then persist for days or weeks. Among the chemical mediators released during inflammation, there are cytokines (TNF-α, IL-1b, IL-6, IL-8) and C-reactive protein (CRP). Acupuncture, a common treatment form in Traditional Chinese Medicine, has a protective effect, decreasing levels of tissue pro-inflammatory cytokine on rats. This study evaluated the inflammatory profile and the pain in 25 volunteers (45± 9 years) with lower back pain treated with systemic Acupuncture or French Auriculotherapy. Materials and methods: Blood was collected and the concentration of pro-inflammatory cytokines [(TNF-α), 1β (IL-1β), (IL6), (IL-8)], anti-inflammatory (IL-1ra), (IL-10) and CRP were analyzed before the treatment, 1 hour after and 24 hours after the Acupuncture or Auriculotherapy treatment through ELISA method. Pain was assessed within the same time interval using the visual pain scale. Results and conclusion: Our results show reduction of the pain. The treatment with systemic Acupuncture techniques reduced the pain by 53% and 50% after 1 hour and 24 hours of treatment, respectively.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP051 ELABORATION OF A PHYTOTHERAPIC GUIDE FOR FARMACIA VIVA OF THE COUNTY OF SOBRAL-CE NATÁLIA CARVALHO RIBEIRO; JOAQUIM HORÁCIO DE ARAÚJO NETO; RAPHAEL LUCAS VASCONCELOS SARAIVA; BIANCA FROTA MONTE; MÁRCIA JORDANA ARAÚJO; WILCARE DE MEDEIROS CORDEIRO NASCIMENTO. Instituto Superior de Teologia Aplicada (Inta), Sobral - CE - Brazil. Introduction and objective: The Farmácia Viva Project is a program that integrates pharmaceutical social assistance based on the scientific use of medicinal and phytotherapeutic plants. Although it provides an economy to the state, and a therapeutic alternative, the project faces some difficulties, such as lack of financial resources and especially the lack of printed information about the medicinal plants common in the region and the herbal medicines that are produced and distributed in the county of Sobral, which makes it difficult to prescribe them. The development of an herbal medicine guide will contribute to greater safety when prescribing them and guiding their use. In view of the objective of a quality pharmaceutical care application to ensure the rational use of medicines, through this research a phytotherapeutic guide was developed with all phytotherapies available to the community, containing indications of use, posology and contraindications, considering that several health professionals show insecurity and doubt when prescribing herbal medicines and/or medicinal plants as a therapeutic option. Materials and methods: The study sample consisted of the medicinal plants and phytotherapies produced in the county of Farmácia Viva Project, as well as the professionals who work in Primary Health Care, who are actively related to the population. Data collection was done through a bibliographic review in scientific databases such as: Scielo, PubMed, BVS, besides the existing phytotherapeutic guides in the counties of Viçosa do Ceará and Maracanaú. Results and conclusion: Besides the economy, the use of phytotherapies decreases the risks of adverse reactions and drug interactions to the patient. Through the conclusion of this research project and with the end of the elaboration of the Phytotherapy Guide for the Farmácia Viva of Sobral, it was possible to implement actions and strategies for the problem of insecurity and doubt that many health professionals presented when prescribing medicinal plants and phytotherapies as a therapeutic option. Financial support or fellowship: INTA

EPIDEMIOLOGICAL ANALYSIS OF CHIKUNGUNYA FEVER IN THE STATE OF CEARÁ

CFSP052

LÍVIA SOUSA UCHÔA; ANA LOUISE DE QUEIROZ OLIVEIRA; CARLA LARISSA DE CASTRO VIEIRA; KAROLINE LEAL MOTA; MARESSA RODRIGUES CAVALCANTE; RENATA ISABELLA ALVES FILGUEIRA SAMPAIO; LARA THAMYRES AZEVEDO DAMASCENO; ENI TEREZINHA FLECK DE PAULA PESSOA. Universidade de Fortaleza, Fortaleza - CE - Brazil. Introduction and objective: Chikungunya fever is a disease transmitted by the bite of female mosquitoes. Its main vectors are the Aedes aegypti and Aedes albopictus. It was initially isolated in Tanzania in 1953, in an outbreak which was associated to the dengue virus. The first case of Chikungunya in Brazil occurred in June 2014, being an export case. The first case in the state of Ceará was also detected in 2014, in a town called Brejo Santo. Our objective is to analyze the epidemiological values of Chikungunya fever in the state of Ceará. Materials and methods: A systemic review of published studies, epidemiological reports and the Ceará state health department database. The reports were analyzed based on the monitoring of Chikungunya fever cases in the years of 2016 and 2017 in the state of Ceará. Results and conclusion: In the period of 01/01/2016 to 12/31/2016 48,085 suspected cases of Chikungunya fever were reported in the state of Ceará. Of these 29,837 (62.0%) were confirmed. The disease attacked, mainly, adults between 30 and 39 years old, with the predominance of females. 508 cases were confirmed in pregnant women. In 2016, up to the 52nd epidemiological week, 19 deaths had been confirmed. Of these 11 were female and 8 male, they were between 12 and 89 years-old. In 2017, up to the 16th epidemiological week, a growing tendency in the number of reported cases is being noticed, reaching an incidence rate of 909.9 cases per 100,000 inhabitants. Up to the 24th epidemiological week, 81,557 cases had been reported, of those 38,959 were confirmed. The disease was concentrated in the 20 to 59 age group, keeping a female dominance with exception of babies less than one year old and the 5-to-14 age group. In 2017, 33 Chikungunya fever deaths were confirmed. Of these, 16 were male and 17 female, they were between the ages of 10 days to 92 years old.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

EPIDEMIOLOGICAL ASPECTS OF INTOXICATION BY MEDICINES IN THE STATE OF RONDONIA

CFSP053

JHENYS DOUGLAS NUNES DA SILVA; FABIA MARIA PEREIRA DE SÁ ZEFERINO; ROSICLER BALDUINO NOGUEIRA; ENEIAS MARCELINO DA ROCHA. Faculdades Associadas de Ariquemes-Faar, Ariquemes - RO - Brazil. Introduction and objective: Medicines are heavily consumed health goods, and the market and productive context in which they are inserted brings health risks closer to their own, which is related to their misuse and may cause various types of poisoning. Thus, the objective of this study was to conduct an epidemiological survey on drug intoxication in the State of Rondonia between 2010 and 2015, through the DATASUS platform of the Ministry of Health Materials and methods: A literature review was carried out, followed by a descriptive study, the study sample of which consisted of drug intoxication data recorded in the SIM/MS. Data were collected in April 2017 on the website of the Department of Informatics of the Ministry of Health (DATASUS), which provides the information sought in this study. Thus, the distribution of drug intoxication between 2010 and 2015 was analyzed in relation to epidemiological data, such as gender, age, schooling, and type of intoxication Results and conclusion: Between 2010 and 2015, 1,349 cases of poisoning were recorded in the state of Rondonia, of which 23.57% were caused by drugs (N = 318). Women account for the largest number of accidents, about 62.26% of cases. The age group of 1 to 4 years and 20 to 39 years correspond to 27.04%, related to the phase of orality of children and attempted suicide in adulthood respectively. In this way, more attention is needed from the pharmaceutical industry in producing safer packaging for children’s medicines, and a greater awareness of the state of health, with emphasis on pharmaceutical assistance in search of a better perspective of medication education for the general population. CFSP054 EPIDEMIOLOGICAL PROFILE OF MEN WITH PROSTATE CANCER AT THE CENTER OF ONCOLOGY OF SÃO JOÃO DA BOA VISTA GISELE FERNANDA DOS SANTOS1; ANA PAULA SENDÃO OLIVEIRA1; DANYELLE CRISTINE MARINI2; FRANK FERREIRA PINTO3. Unifae, São João da Boa Vista - SP - Brazil; 2Unifae, Mogi-Guaçu - SP - Brazil; 3Unifae, São Paulo - SP - Brazil.

Introduction and objective: Prostate cancer is the second leading cause of cancer death in men in Brazil. The prostate is a glandular and fibromuscular organ, located within the pelvic cavity. Most carcinomas show no signs and symptoms. The objective of this research is to characterize the epidemiological profile, as well as to identify the risk factors, initial diagnosis and to evaluate the association between relapse and survival of men with prostate cancer. Materials and methods: This is a descriptive cross-sectional retrospective study approved by the Ethics Committee of UNIFAE with the CAAE 66664917.3.0000.5382. Patients were evaluated with the help of a questionnaire assessing socioeconomic, urological, personal and family history, main risk factors, initial diagnosis and treatment established. Results and conclusion: A total of 30 male patients were studied during the study, 77% were non-smokers, 10% had other types of neoplasia, 37% reported other types of cancer in relatives, 97% do not drink alcohol, 87% did not need surgery, 94% do not need chemotherapy. There is a need to improve the preventive diagnosis in men who have reports of familial cancer, and programs that allow greater speed to the beginning of treatment, since delays in the therapy can lead to aggravations in the disease process. With the data obtained, it is intended to guide municipal policies in the implementation of preventive campaigns and treatment of prostate cancer.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP055 EPIDEMIOLOGICAL PROFILE OF TUBERCULOSIS CASES NOTIFIED IN THE MUNICIPALITIES OF COSTA DAS BALEIAS, BAHIA STATE, BRAZIL, IN THE PERIOD 2007 TO 2016 MYCHAEL EMANUEL RIBEIRO DOS SANTOS1; RAFAEL ANTUNES FERREIRA2; ALINE DA SILVA PEREIRA1. Faculdade Pitágoras, Teixeira de Freitas - BA - Brazil; 2Faculdade do Sul da Bahia, Teixeira de Freitas - BA - Brazil.

Introduction and objective: Tuberculosis (TB) is an infectious disease caused by the bacterium Mycobacterium tuberculosis. It is estimated that 9 million people develop active TB each year, and that infections result in more than 2 million deaths annually. Objectives: To identify and analyze the epidemiology of reported cases of tuberculosis, by consulting the Department of Informatics of the Unified Health System (DATASUS), in the municipalities that make up the Costa das Baleias, Bahia, Brazil, from 2007 to 2016. Materials and methods: A descriptive epidemiological study was carried out and the information was obtained from a database available from DATASUS. The study population consisted of patients reported in the municipalities of Alcobaça, Caravelas, Prado, Mucuri and Nova Viçosa, which make up the Costa das Baleias region, and analyzed for quantitative and qualitative variables. Data were presented and analyzed through the absolute and percentage frequency of reported cases. Results and conclusion: During the period 2007-2016, 584 cases of TB were reported in DATASUS, of which 71 were reported in 2007, 68 in 2008, 66 in 2009, 71 in 2010, 62 in 2011, 54 in 2012, 57 in 2013, 69 in 2014, 52 in 2015 and 14 in 2016. There was a higher prevalence of males and aged between 20 and 59 years. More than 80% of the patients were of the black or brown races, with low schooling and living in rural areas. There was a low prevalence of the disease among the declared alcoholic patients, and only 24 cases of tuberculosis of the 584 were HIV positive. The results obtained make it possible to know the characteristics of the reported cases of TB, as well as to highlight the importance of the analysis of secondary data in order to develop public health interventions in the southernmost region of Bahia. CFSP056 EPIDEMIOLOGICAL PROFILE OF WOMEN WITH BREAST CANCER AT AN ONCOLOGY CENTER IN SÃO JOÃO DA BOA VISTA, BRAZIL BIANCA CORREA DA SILVA1; DANYELLE CRISTINE MARINI2; ANA PAULA SENDÃO OLIVEIRA3; FRANK FERREIRA PINTO4. Unifae, Vargem Grande do Sul - SP - Brazil; 2Unifae, Mogi Guaçu - SP - Brazil; 3Unifae, São João da Boa Vista - SP - Brazil; Unifae, São Paulo - SP - Brazil.

1 4

Introduction and objective: Breast cancer is the most incident form of cancer among women and accounts for 23% of all cancers in Brazil. The occurrence of breast cancer causes psychological, functional and social impacts on the lives of affected women. The main signs and symptoms are a lump in the breast or armpit, breast pain and changes in the skin covering the breasts. The aim of the present study was to characterize the epidemiological profile of women with breast cancer, identify risk factors, determine the time elapsed between diagnosis and the onset of treatment, and evaluate the association between recurrence and survival. Materials and methods: A descriptive, retrospective, cross-sectional study was conducted after receiving approval from the human research ethics committee of UNIFAE (certificate number: CAAE 62619716.0.0000.5382). Data were collected using a questionnaire addressing socioeconomic characteristics, obstetric-gynecological aspects, family history, risk factors, initial diagnosis and established treatment. Results and conclusion: Thirty women participated in the study, 30% of whom reported having had a miscarriage, 63% reported a family history of breast cancer, 7% reported a personal history of another episode of cancer, 20% were smokers and 37% began treatment 11 months after receiving the diagnosis. The findings underscore the need to improve prevention measures in women with a family history of breast cancer and establish earlier treatment, since delayed therapy can aggravate the disease process. Thus, municipal policies are needed for the establishment of prevention campaigns and the treatment of breast cancer.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

EPOETIN ALFA RESPONSE IN HEMODIALYSIS PATIENTS

CFSP057

NATÁLIA MILLENA DA SILVA1; ACSA LIMA MARINHO FONSECA DE MELLO2; ARACELLY FRANÇA LUIS2; TIBÉRIO CEZAR DE SOUZA FIGUEIREDO2; VICTOR AVELINO DE ALMEIDA2; VALDEMIR CORDEIRO DE PAULA2; FERNANDO JOSÉ MALAGUEÑO DE SANTANA1. Universidade Federal de Pernambuco, Recife - PE - Brazil; 2Hospital das Clínicas/Universidade Federal de Pernambuco (HcUfpe), Recife - PE - Brazil. 1

Introduction and objective: Anemia is a common complication of Chronic Kidney Disease (CKD) caused primarily by decreased renal synthesis of erythropoietin (EPO). Epoetin alfa is a hormone biologically equivalent to endogenous EPO, which acts by stimulating the production of erythrocytes. Although this drug is effective, a high proportion of patients do not reach the hemoglobin levels (Hb between 11 and 12 g/dL) recommended by the Brazilian Clinical Protocol and Therapeutic Guidelines for Anemia management in CKD. The objective of this study was to evaluate how many patients on hemodialysis are not at the recommended levels of Hb for CKD. Materials and methods: This is a descriptive observational cross-sectional study conducted in the nephrology sector of HC-UFPE after approval by the Research Ethics Committee under CAAE nº 65514417.0.0000.5208. The sample included all patients on hemodialysis using epoetin alfa for at least 3 months. Through the laboratory exams, it was possible to relate which patients were above, below or within the recommended levels of Hb. Results and conclusion: Of the 39 patients studied, 61.53% had Hb levels lower than 11 g/dL, 11 men and 13 women. Of the total, 35.89% presented Hb levels within the recommendations, being 6 men and 8 women. Only 1 patient had Hb levels above 13 g/dL. Our findings emphasize the need for monitoring Hb levels in order to suggest changes in doses to achieve better possible results of epoetin alfa of these patients. CFSP058 PRESCRIPTION AND ADMINISTRATION ERRORS OF INJECTABLE ANTIMICROBIALS IN A PUBLIC HOSPITAL IN BAHIA IONARA VIEIRA ROCHA DA MOTA1; NARA JACQUELINE SOUZA DOS SANTOS*1; LUCAS BRASILEIRO LEMOS1; PAULO HENRIQUE RIBEIRO FERNANDES ALMEIDA2; GISELE DA SILVEIRA LEMOS1. Universidade Estadual do Sudoeste da Bahia, Jequié - BA - Brazil; 2Universidade Federal de Minas Gerais, Belo Horizonte MG - Brazil. 1

Introduction and objective: The inadequate use of antimicrobials has been a major concern worldwide, as it is the pharmacological class most involved with medication errors. The objective of this study was to analyze the main errors that occurred in the prescription and administration of injectable antimicrobials in a public hospital in the interior of Bahia. Materials and methods: A cross-sectional study carried out in a public hospital, in which antimicrobial prescriptions and administrations were monitored for patients admitted to the Intensive Care Unit (ICU) and Medical Clinic (CM) from November 2015 to February 2016. This study was Approved by the CEP of the State University of Southwest of Bahia. Results and conclusion: We analyzed 43 prescriptions and administrations, of which 30.2% (13) were in the ICU and 69.8% (30) in the CM. In CM, it was verified that the age and pharmaceutical form were not completed in 56.7% and 50% of the prescriptions. The lack of information on the hospitalization unit was also observed in 33%, absence of the bed in 23.3% In addition, the lack of information on weight, medical record number and duration of the treatment was verified in 100% of the prescriptions of the ICU and CM. Among the errors observed during administration were: administration of the incorrect dose in 16.7%, non-identification of the patient in the bed in 33.3% in CM and incorrect time in 36.7% and 7.7% in CM And ICU, respectively. The results obtained in this study demonstrate failures in the completion of mandatory items in the prescription, and the most frequent medication errors. The completeness of all data in a prescription is necessary to avoid medication errors or any other problem related to drug therapy. Financial support or fellowship: UESB; FAPESB

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP059 ESKAPE GROUP BACTERIA INCREASES MORTALITY RATES IN ICU PATIENTS WITH SEPSIS – CASE CONTROL STUDY SONIA TIEMI KOBA1; ALAN MAICON DE OLIVEIRA2; CAMILA SCHULTZ CORDEIRO1; JOSÉ VICTOR DE MIRANDA PEDROSO1; MARIA INES DE TOLEDO1; FERNANDO DE SÁ DEL FIOL1; MARCUS TOLENTINO SILVA1; LUCIANE CRUZ LOPES1. University Of Sorocaba (Uniso), Sorocaba - SP - Brazil; 2São Paulo State University (Unesp), School Of Pharmaceutical Sciences, Araraquara - SP - Brazil. 1

Introduction and objective: Sepsis is a worldwide health problem with high rates of mortality (64%). Therefore the objective of this study is to analyze predictive factors related to mortality in patients with sepsis and septic shock in the ICU in Brazil. Materials and methods: Case-control study conducted in hospitals located in São Paulo State, Brazil. The cases and controls were identified from medical records of patients admitted to the ICU from 2010 to 2013 who were diagnosed with sepsis or septic shock defined according to the Surviving Sepsis Campaign. Cases were defined as those who had died during hospitalization, and controls were defined as those who had survived. The following variables were investigated for the greatest chance of mortality: age, sex, comorbidities, complications, multidrug resistance, the presence of more than three bacteria and the use of more than three antibiotics. Odds Ratio (OR) was used as a measure of association, 95% CI and significance level of 5%. Results and conclusion: Of the total of 201 patients that met the inclusion criteria, 118 (58.7%) had died. The average length of stay in ICU and hospital in days were 22.4±23.2 and 33.5±26.6 for the case group; 24.2±21.6 and 40.7±28.8 for the control group, respectively. APACHE II scores ≥ 25 was predominant in the case group (54.5%). Considering the analysis of adjusted predictive factors, acute kidney failure complications [OR 1.88 (1.04-3.40) p=0.037] and the presence of ESKAPE group bacteria [OR 1.98 (1.08-3.63) p=0.026] were found as predictors related to mortality. CFSP060 EVALUATION OF CARDIOVASCULAR RESPONSES IN UNIVERSITY STUDENTS SUBMITTED TO PSYCHOLOGICAL STRESS MAYSA ZUCON DA SILVA1; TAÍZ FRANCINE BRASIL DA SILVA2; MILENA DEOLINDO VIEIRA1. UNIP, Araçatuba - SP - Brazil; 2USP, Ribeirão Preto - SP - Brazil.

Introduction and objective: Fear of public speaking is characterized as a social anxiety subtype, promoting cardiovascular, neuroendocrine and metabolic responses. Considering the neurobiological aspect, it is possible to point out a relation between the sympathetic nervous system (SNS) activity, emotions and blood pressure (BP). When stimulated, the SNS prepares the organism for a “fight or flight” reaction causing characteristic physiological changes, such as BP and heart rate (HR) increases evoked by aversive situations. Objective: To evaluate academic student’s cardiovascular responses when submitted to public speaking. Materials and methods: A cross-sectional and descriptive study composed of 30 academic students aged from 18-60 years, of both genders and students at a private university in Araçatuba city, state of São Paulo. Results and conclusion: The subjects presented a mean systolic BP of 116.6 ± 2.4 mmHg, mean diastolic BP of 75.5 ± 1.8 mmHg and mean HR of 76.6 ± 2.3 bpm before the public speaking. The public speaking increases the mean systolic BP (146.7 ± 2.4 mmHg, F29,58 = 5.9, p0.05), suggesting that HR returned to normal faster than BP. These results suggest that public speaking affected BP and HR, corroborating previous literature data. Interesting, similar cardiovascular responses were observed in academic students who made use of certain types of medicines. Financial support or fellowship: UNIP; USP

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP061 EVALUATION OF CORRECT USE OF MEDICINES BY PATIENTS AT UNIVERSITY HOSPITAL AT BELÉM-PARÁ LETICIA HIROMI OHASHI; AMANDA ROBERTA PALHETA DA SILVA; MARIA FANI DOLABELA. Universidade Federal do Pará, Belém - PA - Brazil. Introduction and objective: Rational drug use is defined as a process that includes appropriate prescription, timely availability and affordability, dispensing under appropriate conditions and the set of indicated doses at defined intervals and at the indicated time period of effective drugs, insurance and quality. There are many factors that trigger self-medication, such as lack of educational programs, the low purchasing power of the population, family instructions and ease of acquisition, among others. Objective: Obtain information about the knowledge and use of medicines by health service users and also provide them with important information about the risks of inappropriate use of medicines. Materials and methods: Initially, a questionnaire with multiple-choice questions on the use of medication was applied to patients at a university hospital in Belem, Pará. This study involved 150 patients attending the hospital who were randomly chosen. Results and conclusion: This study had 150 patients, of whom 55 (64.5%) were female and 55 (35.5%) were male. About 14.2% of the interviewees reported not seeking to see their doctor when they are ill and about 29.0% (45 patients) seek the doctor sometimes. 67.1% of respondents reported having made use of drugs by recommendation from friends, relatives or neighbors. About 47.7% (74 respondents) of respondents ingested drugs with beverages other than water, such as milk, alcoholic beverages, tea, coffee or juice. A total of 28 (18.0%) study participants reported having purchased an over-the-counter antimicrobial drug. From this study, it is noticed that a high number of patients attended by the university hospital make inappropriate use of medicines, which can lead to health problems. Therefore, it is necessary to raise their awareness, because ignorance about the subject puts the health of the patients at risk and leads to the inappropriate use of medicines. Financial support or fellowship: MEC/SESu. CFSP062 EVALUATION OF MEDICAL PRESCRIPTIONS AT COMMUNITY PHARMACIES IN THE CITY OF CAJAZEIRAS - PB. ANNA CLAUDIA MOREIRA AGUIAR1; KELLY SOUZA LIMA2; ANNALU MOREIRA AGUIAR3; ANA EMÍLIA FORMIGA MARQUES4; NATÁLIA BITÚ PINTO2. Fsm, Cajazeiras - PB - Brazil; 2Faculdade Santa Maria, Cajazeiras - PB - Brazil; 3Faculdade de Medicina do Abc Paulista, Santo André - SP - Brazil; 4Universidade Federal de Campina Grande, Cuité - PB - Brazil. 1

Introduction and objective: The prescription is an important means of communication between physicians, pharmacists and patients allowing an assessment of how the drug contributes to the orientation of pharmaceutical care highlighting the receipt, understanding and correct dispensing of medical prescription as being an important role of the professional in the field. This study aims to evaluate the quality of medical prescriptions in community pharmacies in the city of Cajazeiras - PB. Materials and methods: The study was both exploratory-descriptive with a quantitative approach. Three pharmacies were selected where 100 prescriptions were analyzed, from August to September 2012. The form was based on criteria pre-established by Brazilian legislation and WHO. Results and conclusion: Based on the results, it was possible to observe that only 1% of prescriptions dispensed included information on patient age, 2% showed their weight and 36% contained the address of the patient. Antibiotics, NSAIDS and Psychotropic appear among the most prescribed medications. 56% of prescription drugs were ethical, followed by 41% generic. It was found that 44% of revenue was derived from hospital and 35% of prescriptions were for PSF. Most prescriptions were for females and 56% of prescriptions did not specify the duration of treatment. Doctors were responsible for 95% of all prescriptions and only 41% contained the address and telephone contact of prescribers. It is considered that the majority of prescriptions are not evaluated according to the methods recommended by the WHO and by current legislation. It was also found that the legal procedures related to the requirements are not properly met, underscoring the need for greater oversight so that they are fulfilled, thus ensuring the safety and therapeutic efficacy.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP063 EVALUATION OF MEDICINES USED BY ELDERLY PATIENTS AT A PHILANTHROPIC CLINIC OF JUNDIAÍ - SP ROSEMEIRE ALVES DE MATOS1; VERONICA CRISTINA GOMES SOARES1; LEANDRO DOS SANTOS2; HEDER FRANK GIANOTTO ESTRELA1. Centro Universitário Padre Anchieta, Unianchieta, Jundiaí - SP - Brazil; 2Universidade Federal Rural de Pernambuco – Uast/ Ufrpe, Serra Talhada - PE - Brazil. 1

Introduction and objective: The census conducted by the Brazilian Institute of Geography and Statistics (IBGE) in 2010 showed an increase in the elderly population and consequently demand for certain medical specialties and higher consumption of medicines. Often, the elderly use polypharmacy that increases the risk of drug interactions, adverse reactions, drug intoxication, and others drug-related problems. The aim of the present study was to evaluate the medicines used by elderly patients at a philanthropic clinic in Jundiaí, SP.” Materials and methods: Medical records of 80 institutionalized elderly were evaluated. Data on pathologies and medications in use were collected between August and December, 2016. Drug interactions were classified as A, B, C, D and X and drug safety according to the Beers classification. Ethics approval for the study was granted by the Ethics Committee of the UNIANCHIETA (Certificate No. 1.674.111). Results and conclusion: The sample comprised males (26) and females (54), mean age 79 years (range: 60 - 101). Cardiovascular diseases were the most prevalent (68), followed by mental (45) and endocrine (36) diseases. The most commonly used drugs were antihypertensives (126), vitamins (72) and psychotropic drugs (52). There were 302 pharmacodynamic drug interactions and 205 pharmacokinetics drug interactions, risk B (92), C (347) and D (68), there were no interactions A and X. Applying the Beer’s classification, 27 patients used potentially inappropriate medicines for the elderly. The findings from this study show several drug interactions, but moderate risk interactions were most prevalent. However, there are some high-risk interactions and significant use of drugs potentially inappropriate for the elderly. Financial support or fellowship: PIBIC/CNPq CFSP064 EVALUATION OF PHARMACEUTICAL ASSISTANCE IN PRIMARY HEALTH CARE AS A STRATEGY FOR THE RATIONAL USE OF MEDICINES THAÍS MARTINS CARNEIRO SANGIORGI KADENÁ; MÁRIO LUÍS RIBEIRO CESARETTI. Pontifícia Universidade Católica de São Paulo - Pucsp, Sorocaba - SP - Brazil. Introduction and objective: User satisfaction is a way to evaluate the quality of health services, and this information serves to improve the structure, the process and the result, improving treatment adherence and therefore reflecting the rational use of medicines. Objective: Evaluate the current situation of pharmaceutical care provided at the primary level of health care in the city of Sorocaba-SP, as well as the dimensions of the structure, process and outcome, based on the Donabedian Model. Materials and methods: The methodology used in this study was exploratory, analytical and cross-sectional descriptive and quantitative approach. The questionnaire presented semi-structured questions in Likert scale, applied to the pharmacists, pharmaceutical residents of the Multidisciplinary Residency Program and users of the Jardim Simus Basic Health Unit and Municipal Polyclinic of Sorocaba - SP. The user group featured a total of 38 respondents in their own established units, chosen at random. Referring to pharmacists, the full participation was 4 professionals. Results and conclusion: The study obtained a unanimous vote on the responses of users regarding the lack of pharmaceutical guidance given. Pharmaceutical professionals when approached about the capabilities, revealed the absence of professional enhancements. Because of the small number of pharmacists, the statistical calculations had no significance in the outcome. The pharmaceutical care in the municipality is lacking, requiring greater investment in human, physical and material resources. The absence of the pharmacist in the health team is an important factor, and there is a fragmentation in the execution of the steps of the pharmaceutical care cycle. It is believed that these findings may contribute to a possible change in the quality of pharmaceutical services in primary health care, aimed at full care and rational use of medicines.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

EVALUATION OF PHARMACEUTICAL CARE FOR HIPERTENSE PREGNANT WOMEN

CFSP065

MARIANA SUZUKI CARNEIRO; JOSELAINE DE OLIVEIRA; MARIANE MACHADO CURBETE. Universidade Paulista, Araçatuba - SP - Brazil. Introduction and objective: Introduction and objective: Arterial hypertension in pregnancy is characterized as an important public health problem, being the first cause of direct maternal death in Brazil. Prenatal care is essential to assist the woman from the beginning of gestation and ensure her well-being and that of the fetus. Pharmaceutical care is an important ally, promoting in association with the patient, safe and effective treatment, in order to prevent and resolve possible pharmacotherapeutic problems, such as drug interactions and adverse reactions. Objective: To evaluate the pharmaceutical care provided to pregnant women. Materials and methods: Cross-sectional, descriptive, observational and prospective study conducted at the Basic Health Units of the Municipality of Pereira Barreto, Sao Paulo state, using a questionnaire previously elaborated with closed dichotomous multiple choices and semi-open questions that refer to the demographic profile, medicines used and pharmaceutical care offered to 30 pregnant women aged 18 or over. Results and conclusion: The participants who received a diagnosis of Hypertensive Syndrome in Gestation, totaled 10% (n = 3), all of them using methyldopa in pharmacotherapy. Of the 30 participants, 50% (n = 15) received pharmaceutical assistance and considered the orientation of the pharmaceutical professional important. It was concluded that pharmaceutical care is recognized as indispensable and necessary, since there are contraindications in the use of several drugs in high-risk groups, as is the case of pregnant women with Hypertensive Syndrome. CFSP066 EVALUATION OF PHARMACIST INTERVENTIONS IN THE INTENSIVE CARE UNIT OF A PRIVATE PEDIATRIC HOSPITAL IN SÃO PAULO THAIS ALVES LOPES; GABRIELLA CANDIDO DE FREITAS LANDIM; ANAHI AYALLA CÉSAR BASSI. Hospital Infantil Sabará, São Paulo - SP - Brazil. Introduction and objective: The rational use of medication is characterized by the correct use of drug therapy. During evaluation by the pharmacist, it is possible to identity problems related to pharmacotherapy. These problems can be reported to the prescriber, who will analyze the pharmacist’s intervention. Objective: The purpose of the study to identify the prevalence of interventions performed by the clinical pharmacist during the analysis of electronic prescriptions. Materials and methods: A retrospective study was carried out from March 2016 to March 2017. Pharmaceutical interventions were classified into: dosage, administration, duplicates, renal function, drug interaction, drug reconciliation and indication. Results and conclusion: During the study, a total of 9,249 prescriptions were analyzed by clinical pharmacists. It was observed that 61.74% of the performed interventions were related to dosage and 22.5% to indication of medication. Pediatric prescriptions are very complex. The absence of specific formulations and necessity of dose calculation turn prescriptions vulnerable, which makes the presence of the clinical pharmacist indispensable for patient safety and the prevention of adverse events related to drug therapy. Financial support or fellowship: Hospital Infantil Sabará

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

EVALUATION OF PLASMATIC LEVELS OF LITHIUM CARBONATE IN CHRONIC USERS

CFSP067

EVERTON BOFF 1; ALANA CARLA BATTISTELLA 1; RÉGIS CARLOS BENVENUTTI 1; VANESSA DA SILVA CORRALO2. Unoesc, São Miguel do Oeste - SC - Brazil; 2Unochapecó, São Miguel do Oeste - SC - Brazil.

Introduction and objective: Lithium carbonate is the first-choice drug for the treatment of bipolar disorder, however, the therapeutic dose of this drug is extremely close to its toxic level requiring frequent biological monitoring. The objective of this study was to evaluate the toxicity of lithium carbonate in chronic users. Materials and methods: A transversal, quantitative study in which participants were aged over 18 years and had been receiving lithium carbonate treatment for at least 6 months. The study was approved by the Research Ethics Committee, certificate No. 1.966.155. The interview was semi-structured. Blood samples were obtained preferably 12 hours after the last dose and with the subject preferably fasted (but not mandatory). The lithium dosages were performed using the Colorimetric Immunoenzyme method. Results and conclusion: Of the 25 lithium users, 19 were women, aged 41 to 45 years, retired and with incomplete elementary school. Many had been taking the medicine less than 2 years. It may be noted that some initial symptoms were persistent, but not in all patients. The most concomitantly used drugs were antidepressants and antipsychotics. The majority presented levels below 0.59mEq/L, showing that some failure is occurring, possibly due to the patient’s incorrect report or the dose being inadequately low. In this way, monitoring the lithium carbonate users is necessary to avoid possible toxicity or therapeutic failure. CFSP068 EVALUATION OF POSSIBLE DRUG INTERACTIONS IN PATIENTS UNDER SYSTEMIC LUPUS ERYTHEMATUS TREATMENT RAIRA MACARIO SILVERIO; LUCIANA RESENDE PRUDENTE; NATHALIE DE LOURDES SOUZA DEWULF. Universidade Federal de Goiás, Goiânia - GO - Brazil. Introduction and objective: drug related events are major issues in healthcare. Among such events are drug interactions (DI), which may compromise treatment and pose a threat to the patient. The occurrence of DI is common in multidrug therapy, such as those used to treat systemic Lupus erythematous (SLE). The objective of this work was to identify possible drug interactions (PDI) in patients under SLE treatment in a university hospital. Materials and methods: Medical reports of 38 SLE patients were collected and analyzed using Micromedex database and following the classification of this database. The interactions classified as important due to being potentially fatal and/or requiring medical intervention to decrease and avoid serious adverse effects were considered. This study was approved by the research ethics committee, certificate number: 198.943 HC/UFG Results and conclusion: The average number of drugs in use by SLE patients was 8.9 drugs. Of those, the PDI added up to 86% drug/drug (d/d), 79% drug/food (d/f), 22% drug/alcohol (d/a) and 10.8% interactions of drug/tobacco (d/t). Between interactions considered important, presenting a potential risk to the patient, 94% d/t, 54% d/d, 14% d/a, 11% d/f. The results show considerable numbers of PDI, therefore indicating the need of intervention to help minimize risks of PDI in multidrug therapy and the importance of interaction data in clinical protocols for SLE patients. Financial support or fellowship: LaPESF, UFG

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP069 EVALUATION OF POTENTIAL PROBLEMS OF PRESCRIPTIONS SERVED IN A PHARMACY THAT MAY INDUCE ERROR OR CONFUSION IN DISPENSATION KARINA MARQUES BOTONO1; GLEISE DA SILVA BELIZARIO1; MÔNICA MIYUKI TAKAHASHI1; JULIANA CRISTINA DO NASCIMENTO2; VALTER LUIS DA COSTA JUNIOR1; LUCIANE MARIA RIBEIRO NETO1. Centro Universitário São Camilo, São Paulo - SP - Brazil; 2Centro Social Nossa Senhora do Rosário, São Paulo - SP - Brazil.

Introduction and objective: Preventable morbimortality related to medication is a frequent problem found in health systems, with medication errors being one of the main causes. Therefore, the objective was to identify the potential problems related to the prescription attended in a pharmacy without manipulation that could induce error or confusion. Materials and methods: The observational pilot study evaluated prescriptions at a non-compounding pharmacy in the period from March 20 to May 19, 2017. The analysis of prescriptions focused on medical prescriptions served at this facility, quantified and classified into handwritten or typed. The requirements assessed by both a pharmacy student and a pharmacist were: legibility, unit of measurement, use of abbreviations and duration of treatment. Results and conclusion: Of the 1,143 prescriptions evaluated, 60.8% were handwritten and 2.4% did not meet some of the requirements surveyed, of which 82.1% were handwritten and 78.6% did not meet 2 or more requirements. The main problem identified was related to the requirement unit of measurement (92.6%), followed by legibility (60.7%) and duration of treatment (32.1%). Although the main problem identified in this study was related to the unit of measurement, because it is a non-compounding pharmacy, the problem related to the lack of readability becomes more relevant due to the possibility of inducing error or confusion as well as, making dispensing impossible. It was also possible to verify that even the prescriptions entered did not meet all the evaluated requirements. Thus, the awareness of health professionals and patients becomes fundamental in reducing medication errors and consequent contribution to patient safety.

EVALUATION OF THE ANTIMICROBIAL ACTIVITY OF OIL-RESIN OF COPAIFERA SP.

CFSP070

AFONSO RAFAEL NASCIMENTO E SILVA1; DANILA TERESA VALERIANO ALVES1; JESSICA SILVA SOUSA2; MARIA JOSÉ SANTOS DE QUEIROZ 2; SANDRYNNE CARLA NEVES GUIMARAES 1,2; CHRISTIAN NERI LAMEIRA1,2. Faculdade Integrada Brasil Amazônia, Belém - PA - Brazil; 2Escola Superior da Amazonia, Belém - PA - Brazil.

Introduction and objective: Due to the great potential of Amazonian flora, it is important to determine the activity of several vegetal components and the focus of this work was the study of the oleoresin of Copaifera sp., which was inserted in an expressive form in the Amazonian communities. The study covers its chemical characteristics as the main factor in producing specific responses. Objectives: To determine the antimicrobial activity of copaiba oleoresin (Copaifera sp.) and to observe among the concentrations used those capable of inhibiting microbial growth. Materials and methods: The experiments were conducted in the multi-analysis laboratory of the Escola Superior da Amazonia ESAMAZ and the copaiba oleoresin was obtained in the city of Cameta, PA. The tests for the evaluation of the inhibitory activity of copaiba oil were carried out with standard strains of Escherichia coli - ATCC 8739, Pseudomonas aeruginosa - ATCC 9027 and Staphylococcus aureus - ATCC 25.923. Results and conclusion: It was concluded that Copaifera sp. oleoresin, when evaluated at 10% concentration, showed total inhibitory activity of the gram positive strains of Staphylococcus aureus. Concerning the activity in gram negative strains of Pseudomonas aeruginosa and Escherichia coli, the oil had a moderate inhibitory action. The results present a significant contribution to the characterization of the antimicrobial activity of copaiba oleoresin, widely used in folk medicine.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP071 EVALUATION OF THE BACTERICIDAL POTENTIAL OF THE ETHANOL SOLUTION 70% (V/V), USED AS DISINFECTANT AGAINST DIFFERENT BACTERIAL SPECIES MAYARA CONTI PIMENTA; ALEX JUNIOR FERREIRA; EDUARDO CARNEIRO CLÍMACO. Universidade de Ribeirão Preto – Unaerp, Ribeirão Preto - SP - Brazil. Introduction and objective: Surface cleaning and disinfection play an important role in the prevention and control of healthcare-related infections, since they guarantee environments less contaminated with potentially pathogenic microorganisms and therefore more appropriate to good practices for the provision of health services. In this way, it is essential to improve effective techniques to promote the cleaning and disinfection of surfaces in hospitals or similar environments. The present study evaluated the antimicrobial activity of 70% (v/v) ethanol at 20ºC, used as a disinfectant agent for granite surfaces, against bacterial strains Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853. Materials and methods: For this purpose, serial dilutions (10-1 to 10-6) of the bacterial strains were prepared in saline solution, starting from a turbid solution standardized at 1.2 ± 0.1 Abs at 600nm. The 2 milliliter volume of each dilution was applied over fields marked on the surface of granite stones sterilized for 10 min. After this period, excess bacterial inoculum was removed with sterile gauze. The surfaces of the contaminated stones were disinfected with 70% v/v ethanol by two methods: (i) submersion and (ii) submersion followed by friction. Some were not disinfected to serve as controls. Samples of the stone surfaces were collected with moist swab, seeded on Mueller Hinton agar and incubated at 37ºC for 24 hours. Results and conclusion: By analyzing cultures from non-disinfected surfaces, it can be inferred that the method used recovered the three lines investigated well, in proportions proportional to the bacterial dilutions used to contaminate the stone surfaces. By analyzing cultures from the disinfected surfaces, it was observed that both E. coli ATCC25922 and P. aeruginosa ATCC27853 were completely inactivated by 70% ethanol, both when used by submersion and by submersion followed by rubbing. However, S. aureus ATCC25923 was inactivated by ethanol only by friction and not by 70% ethanol in submersion. Therefore, the use of 70% v/v ethanol was effective for the three types of bacteria investigated when used by friction, inactivating Staphylococcus aureus ATCC25923, which showed the 70% ethanol resistant bacterium among those that were investigated. Financial support or fellowship: Universidade de Ribeirão Preto – UNAERP/SP CFSP072 EVALUATION OF THE CAPILLARY GLYCEMIA TESTS CARRIED OUT IN “PHARMACIST IN THE SQUARE” ACTIONS IN THE MUNICIPALITY OF BAURU/SP. MARIA BENEDITA ESGOTTI. Hrac-USP-Bauru, Bauru - SP - Brazil. Introduction and objective: The present study showed from the data compiled that a considerable number of people had high results in screening tests for capillary glycemia and were unaware. Illness that can silently damage various organs and becomes noticeable in advanced stages. The screening test performed at these events was considered of extreme importance for the population, since tests screened as outside of the rate considered normal (70 to 99 mg/dL) were referred for an early diagnosis. With this clinical and laboratorial determination also recorded, they could interrupt the progression of the disease and its consequences. Screening individuals with risk factors such as obesity and family history of diabetes was also the focus of this screening, as early treatment could prevent future chronic complications in these individuals. Materials and methods: EPI’s for the procedure; Dry cotton; 70% Alcohol Sachets; Chip/tapes; Lancet;Glycosimeter. Preparation of the material to be used; Orient the patient in relation to the technical procedure; Use PPE for execution; Prepare the gaugemeter for gauging; Choose one of the iris that has a good peripheral perfusion. Open the lancet housing; Hold the nail, making slight

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

pressure for “blood pool” on the spot; Do sepsis with sachet 70% alcohol; Take a superficial prick with the lancet; Place a drop of blood until the test site is completely filled; Press dry cotton to the puncture site; Wait for the test result to read on the device. Discard material used in proper disposal container; Record the result on the patient’s own chart, inform and guide the patient regarding the result obtained in the test. For such determinations, a portable glucose meter was used, following the recommendations of the manufacturer, the device demonstrated precision in determining glycemic levels during the event, presenting advantages in that it was an easy-to-perform test that provides results in a few seconds. Capillary glycemia monitoring is now considered a prime factor for good glycemic control. Results and conclusion: Considering current reference values according to the American Diabetes Association (ADA) and the Brazilian Society of Diabetes (SBD) and other similar organizations, we adopted as reference values: Normal: fasting 70 - 99 mg/dL; High: Fasting 100-125 mg/dL; Diabetes: Fasting> 125 mg/dL. In the evaluations performed on 500 patients, fasting and without use of anti-glycemic drugs, we had an elevated finding of patients in the 100 to 125 and above 125 mg/dL range. The determinations were considered essential for the screening and referral of these patients for laboratory and clinical monitoring. For patients with results from 100 to 125, some parameters that we considered important at the time of the pharmaceutical consultation were: control of food consumption (quality, quantity and regularity of meal times); Regular physical exercise; Pica et al., 2003), and the best way to reduce the complications associated with changes in glycemia (Pica et al., 2003) is trying to maintain its level in normal concentrations (Bush, 2004), and to do this it is necessary to carry out measurements of its quantity (Pica et al., 2003). Concentrations should remain within a safety range fasting may be between 70 and 99 mg/dL. Considering that the number of diabetics is increasing due to population growth and aging, greater urbanization, progressive prevalence of obesity and sedentary lifestyle, we conclude that the results showed that the population urgently needs to change their eating habits and seek motivation to care for their health.

EVALUATION OF THE ELDERLY PATIENTS PROFILE IN PHARMACEUTICAL CARE

CFSP073

ANA CAROLINA NASCIMENTO1; MARISTELA FERREIRA CATÃO CARVALHO2. Faculdade de Medicina do Abc, Santo André - SP - Brazil; 2Instituto de Geriatria e Gerontologia José Ermírio de Moraes, São Paulo - SP - Brazil. 1

Introduction and objective: Pharmaceutical Care is the direct interaction between the pharmacist and the user, seeking a rational pharmacotherapy and defined and measurable results, aimed at improving quality of life. Thus, Pharmaceutical Care is a relevant tool in the treatment of elderly patients because this population has biopsychosocial characteristics and consequently a complex treatment regimen. Objective: to evaluate the profile of elderly patients seen in Pharmaceutical Care. Materials and methods: Descriptive, observational study of 91 elderly individuals attended at the Pharmaceutical Care service of the “Instituto Paulista de Geriatria e Gerontologia José Ermírio de Moraes” (IPGG-JEM) from January to April 2015. The data were collected by means of a standard form filled in after consultation of pharmaceutical care and by consulting the medical records of patients selected according to the inclusion and exclusion criteria. The Stata 9 programs for inclusion and processing of data were used. Results and conclusion: 91 patients participated in the research with a mean age of 76.08 ± 6.15 years, 72.53% female and 27.47% male; average school education of 4.20 ± 3.33 years. It was observed that among the women, 54.55% were widows, while only 16.00% of the men were widowers. The most prevalent medical conditions were hypertension (90.11%), hyperlipidemia (72.53%) and diabetes (40.66%). In 39.56% of the elderly, self-medication was observed. Mean of 9.02 ± 3.39 prescription drugs. 23% of patients had serious drug interactions. There was also a non-adherence to drug treatment in 82.42% and polypharmacy (use of 5 or more drugs) in 93.41% of the elderly. This study achieved its objective with regard to socio-demographic, epidemiological and pharmacotherapeutic description of elderly patients in Pharmaceutical Care demonstrating the importance of gerontological vision of health professionals to improve assistance to this population.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

EVALUATION OF THE FREQUENCY OF DRUG INTERACTIONS INVOLVING DRUGS

CFSP074

GUSTAVO ALVES ANDRADE DOS SANTOS1; CLAUDINEI ALVES SANTANA1; PAULO CELSO PARDI2. Senac, São Paulo - SP - Brazil; 2Anhanguera, São Paulo - SP - Brazil.

Introduction and objective: In Brazil, people aged 60 years and over are considered elderly. Comorbidities in the elderly are especially common with advancing age when a series of physical and mental disorders occur simultaneously. Physical health problems not only coexist with mental disorders such as depression, but can also predict the onset and persistence of other diseases. Alzheimer’s disease (AD) is the most common form of dementia, recognized as being accompanied by abnormal neuronal activity associated with beta-amyloid deposition between neurons. Drug interaction is given by the possibility of one drug when given alongside another, changing the intensity of the pharmacological effects of one of them. It is a phenomenon that is expressed inside the body, when the medicines administered to the patient begin to exert clinical interferences on each other. In elderly people with dementia, specifically Alzheimer’s disease, the occurrence of drug interactions has been frequent. Our work seeks to identify and classify the main drug interactions in institutionalized elderly in a clinic in the city of Cuiabá-MT. Materials and methods: A retrospective analytical descriptive study was carried out through the collection of data in a standardized instrument on the use of drugs in patients with Alzheimer’s Disease (AD). The medical prescriptions of 51 patients, both sexes, with a proven diagnosis of AD, who were institutionalized in the months of April and May of 2015, at the Mato Grosso Geriatrics Center. The instrument used to identify the type of interaction was the MICROMEDEX base. Data analysis was performed using the SPSS 17 statistical program. The project was submitted to the Brazil Platform and approved under number 61668016.5.0000.5541 on 11/11/2016 Results and conclusion: In the research, 51 patients were included, of these 42 used drugs, 37 (88%) using 02 or more drugs and 5 (12%) using only 1 drug. Of the 37 patients with possibility of interaction, in 12 patients (32%) there were no drug interactions described in the literature. In 48 patients (68%), 48 interactions were observed, with 26 moderate (54%) interactions (54%) and 22 severe interactions (46%). Among the 26 moderate interactions, the mean age of the patients was 80 years, being 10 (55%) men and 8 (45%) women. Among the 22 serious interactions, mean age 80 years, 8 (47%) men and 9 (53%) women Most of the interactions are of moderate severity, of which the mean age is 80 years, with 55% of the patients belonging to the male sex. The results confirm the need for pharmacotherapeutic follow-up in patients with AD, mainly due to the complexity of the drugs used. CFSP075 EVALUATION OF THE KNOWLEDGE AND USE BY STUDENTS OF A HIGHER EDUCATION INSTITUTION IN WESTERN SÃO PAULO OF THE AQUI TEM FARMÁCIA POPULAR PROGRAM BRUNA DINIZ BORDAN; RAFAEL SOUZA SILVA; SERGIO MARCOS DA SILVA; EDNA AMARI SHIRATSU TAKAHASHI. Universidade do Oeste Paulista, Presidente Prudente - SP - Brazil. Introduction and objective: The Farmácia Popular do Brasil is a federal program launched to improve pharmaceutical services. To broaden the scope, it was extended to “Aqui Tem Farmácia Popular”, which provides care in private sector through the copayment system for granting subsidies from federal level and complemented with patient’s financial resources. The relevance of this project is due to the financial factor representing the main difficulty in acquiring medicines and thus to continuing an established treatment. In face of federal budget system instability, the Brazilian Ministry of Heath has reduced copayment system financing of this program. The present study aimed to evaluate the knowledge and use of “Aqui Tem Farmácia Popular” program by students of a higher education institution in western São Paulo.. Materials and methods: Applied quantitative, cross-sectional and descriptive research, through an online questionnaire. Results and conclusion: The work highlights the program’s objectives in increasing access to medicines for the most prevalent diseases, thus reducing the impact on the family budget (81%). About 80% of the participants believe that suspension or cancellation of the program will make access to medicines difficult, and only 38% of the students know about the program. The biggest challenge is to promote not only access, , but also to associate adherence to treatment: the rational use of medicines. Financial support or fellowship: Universidade do Oeste Paulista.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP076 EVALUATION OF THE KNOWLEDGE OF CONTRACEPTIVE METHODS USED BY ADOLESCENTS FROM A PUBLIC HIGH SCHOOL IN THE CITY OF FORTALEZA UMBELINA ROSE DE SOUSA PEREIRA1; JANAINA LOPES DE MELO2; NAIRA LIMA SILVA2; TICIANA PRACIANO PEREIRA3; ANDRÉA BESSA TEIXEIRA3. Graduate in Pharmacy from Faculdade Metropolitana da Grande Fortaleza - Fametro, Fortaleza - CE - Brazil; 2Undergraduate Student in Pharmacy at Faculdade Metropolitana da Grande de Fortaleza - Fametro, Fortaleza - CE - Brazil; 3Professor of Faculty of Pharmacy at Faculdade Metropolitana da Grande Fortaleza - Fametro, Fortaleza - CE - Brazil. 1

Introduction and objective: Knowledge of contraceptive methods and of the risks originating from unprotected sexual relations is essential for adolescents to experience sex in a proper and healthy manner, ensuring the prevention of unplanned pregnancy and sexually transmitted diseases (STDs). The objective of the present study was to analyze the level of familiarity and the usage of contraceptive methods by adolescent students from a high school in the city of Fortaleza. Materials and methods: A descriptive cross-sectional study with a quantitative approach was carried out, emphasizing specific points on the knowledge and usage of contraceptives by the adolescents through the application of a questionnaire. Results and conclusion: Twenty-seven male adolescents and 38 female adolescents participated, ranging from 15 to 22 years old, those 18 years old being the majority among the participants in the study (52.4%). It was observed that 60% had sexual experience and that the first of their sexual relations happened on average between the ages of 14 and 17. Regarding the use of contraceptive methods during their first sexual relation, 59% reported having used some form, primarily male condoms. The principal informational channels used to acquire information about contraceptive methods were the internet (18.2%), television, lectures, school (16.16%), and parental guidance (14.64%), the male condom (35.15%) and the female condom (18.8%) being the contraceptive methods about which the adolescents knew most. Regarding contraceptive methods that simultaneously prevent pregnancy and STDs, it was observed that the most common response was the male condom (48%). Interestingly, 21 adolescents reported the use of other contraceptives, not condoms, as methods of preventing pregnancy and STDs. Therefore, it is important that sexual education involve the guidance of families and schools, and the participation of healthcare professionals, particularly pharmacists, that have knowledge of contraceptive methods and that have the ability to advise.

EVALUATION OF THE MEDICINE ACCESS USED BY ELDERLY PATIENTS, MACAPÁ-AP

CFSP077

SAMARA GRAZIELA GUIMARÃES DA SILVA; NAYARA DOS SANTOS RAULINO DA SILVA; ALINE MARIANA LOPES MARTINS; ANNA ELÁYNE DA SILVA E SILVA; URIEL DAVI DE ALMEIDA E SILVA; MAYARA AMORAS TELES FUJISHIMA; CAROLINA MIRANDA DE SOUSA LIMA. Universidade Federal do Amapá, Macapá - AP - Brazil. Introduction and objective: The principles of the Unified Health System (SUS) and National Drug Policy (PNM) seek to guarantee access to medicine and, to make this happen, both the SUS and the PNM adopt the essential medicine lists, which help in supply and improve pharmacotherapy without compromising the family income of elderly people. The objective of this study was to evaluate the access of essential medicines used by elderly individuals in Macapá-AP. Materials and methods: This pharmacoepidemiological study was developed through an observational, prospective, transversal, analytic, descriptive investigation with non-probabilistic samples. We selected elderly individuals of both sex who are attended in a social center of Macapá city and we utilized one convenience sample of 122 patients through a structured quiz containing 40 questions with demographic, socioeconomic and pharmacotherapeutic variables. The data was analyzed through multiple logistic regression test using the version 5.3 of BioEstat. This study was approved by CONEP. Results and conclusion: The results showed that 98.4% of the patients used some kind of medicine, of which 42.5% of the drugs were on the essential medicine lists (national, state and municipal lists). However, 50% of these patients reported that they usually buy these medicines; 4.9% reported free access to medicines through SUS; 49.1% reported other forms of access like popular drugstore program. The low educational level (variable: illiterate) showed a relevant relationship with the ways that elderly people access the medicines (p=0.0312/α≤0.05). So, we conclude that the access to important medicines for the elderly was essentially by purchase; the elderly individuals with lower educational level had more difficulties in free access, through SUS. This fact may cause an access problem to the therapy and therapeutic effectiveness. Financial support or fellowship: UNIFAP.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP078 EVALUATION OF THE PROCESS OF ADMINISTRATION OF DRUGS VIA ENTERAL CATHETER IN A HOSPITAL ENVIRONMENT GISELE ROMÃO PINTO1; AMANDA DIAS DE ALMEIDA1; ALESSANDRA COUTO BOAVA1; CRISTIANE ELOÍSA VENÂNCIO GUEDES2; ELI CRISTIANO DE MENESES1. Universidade São Francisco, Bragança Paulista - SP - Brazil; 2Hospital Universitário São Francisco, Bragança Paulista - Brazil.

Introduction and objective: In the hospital environment, it is observed that several pathologies may prevent the patient from swallowing drugs and food, often a complex treatment can require a combination of drugs in the prescription; these patients have, as an option, received drug therapy via enteral catheter. In this context, questions arise regarding the process of maceration/crushing of drug and an adequate washing of the probe in use by the patient. The objective of this study is to review the preparation and administration techniques of drugs via enteral catheter in order to comply with good pharmaceutical practices. Materials and methods: For data collection, the nursing team answered a questionnaire about the technique of preparation and administration of drugs via enteral catheter. The data collected helped to create a table explaining the process of preparation and administration of drugs. Results and conclusion: 155 questionnaires were analyzed and 88% reported that as capsules are opened, diluted and administered by catheter, that could influence the effectiveness of drugs; 19% reported the use of distilled water and not potable water at the dilution. Other identified problems such as: non-lavage of the catheter before and after administration, increasing risks of obstruction; Immersed drugs for indefinite dissolution, altering the physicochemical conditions of the product; maceration or dilution of several medicine not even recipes, interacting with each other. A table was delivered to all clinics and the team trained on the correct administration technique. CFSP079 EVALUATION OF THE RATIONAL USE OF HERBAL MEDICINES IN A COMMUNITY PHARMACY IN THE DISTRICT OF ICOARACI-PARA. MYLENA CORREA SILVA; FLÁVIA MARTINS PACHECO FERREIRA; LUIZ EDER DE OLIVEIRA RAMOS; ROSILEIA DO SOCORRO BEGOT OLEGARIO; DANILA TERESA VALERIANO ALVES*; CHRISTIAN NERI LAMEIRA*. Faculdade Integrada Brasil Amazonia(Fibra), Belém - PA - Brazil. Introduction and objective: Phytotherapy consists of the use of medicinal plants in their different preparations for purpose of therapeutic action. However, self-medication and long-term use of these medicaments may determine factors which lead to intoxication and the concealment of evolutionary diseases. Objective: To evaluate the rational use of herbal medicines in a community pharmacy in the district of Icoaraci - Para. Materials and methods: A descriptive study was realized using a questionnaire to interview users of herbal medicines. The users were randomly selected according to the acquisition of herbal medicines from October 2016 to February 2017. The questionnaire presented subjective and objective questions about age, sex, schooling, acquisition and use of the medication. Results and conclusion: The results generated from the sociodemographic profile showed that 75% of the users of herbal medicines are female, between 19 and 60 years of age and medium level schooling. 56% of users say they buy medication from friends and relatives recommendation and 90% of them say they know the purpose of herbal medicine. From this it was concluded that the majority of users had knowledge about herbal medicine. However, it is not possible to affirm that the population of Icoaraci have access to Pharmaceutical professionals and the necessary information on phytotherapy for the efficient execution of rational use. Financial support or fellowship: Faculdade Integrada Brazil Amazonia

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP080 EVALUATION OF THE RESULTS OF THE IMPLEMENTATION OF THE LEAN HEALTHCARE METHODOLOGY IN THE PHARMACY OF A SMALL PEDIATRIC ONCOLOGY HOSPITAL IN THE CITY OF SÃO JOSÉ DOS CAMPOS - SP. AMANDA FERNANDES DA SILVA. UNIP Dutra-São José dos Campos, São José dos Campos - SP - Brazil. Introduction and objective: The Lean concept in the health sector is a process improvement approach that eliminates waste to improve the flow of patients, information or assets, and has been successfully adapted to the health environment, enabling Hospitals to simplify their operations. Lean Healthcare is a Lean adaptation of the Toyota Production System (STP) for application in the healthcare industry. The quality tools of this system are: Heijunka (level programming), 5S (organization and cleaning senses), VSM (value stream mapping), SMED (tool changeover time reduction), Poka-Yoke (Errors), 6 Sigma (elimination of errors), Kaizen (zero waste and continuous improvement), TPM (improvement of existing maintenance system) and Kanban (signaling to do the necessary when necessary). Objective: To evaluate the results of the implementation of the Lean Healthcare methodology at the pharmacy of a small pediatric oncology hospital in the city of São José dos Campos, São Paulo. Materials and methods: In June 2016, the implementation of the Lean Healthcare methodology was started at the hospital pharmacy with the aim of improving the flow of health supplies. The data for analysis of the results and construction of the graphs presented in the results were obtained from the Computerized Hospital Management system. Results and conclusion: The results show that after the implementation of the Lean Healthcare Methodology in the hospital pharmacy, the application of the Heijunka tool obtained a reduction in the purchase and stock values, with the 5S tool a greater organization and cleaning of the sector, with the VSM tool optimized working time, with the Poka-Yoke tool a reduction of errors, and with the Kaizen tool less waste and improvement of the material distribution process. It is concluded that the implementation of the Lean Healthcare methodology in the hospital pharmacy has improved the quality of services provided by this important sector of the hospital. CFSP081 EVALUATION OF THE SOCIOEPIDEMIOLOGICAL PROFILE OF PATIENTS HOSPITALIZED FOR TUBERCULOSIS AT A UNIVERSITY HOSPITAL IN THE CITY OF BELÉM-PA ADRIANA DO SOCORRO UCHOA DA SILVA; FABÍOLLA DE CÁSSIA SOARES CARDOSO; PAULA SOUSA DA SILVA ROCHA; MARCIENI ATAIDE DE ANDRADE; MARCOS VALÉRIO DA SILVA. Universidade Federal do Para - Ufpa, Belém - PA - Brazil. Introduction and objective: Tuberculosis is a serious public health problem in the world, caused by Mycobacterium tuberculosis, affecting all age groups, causing great suffering and social and economic costs, especially in developing countries where it is exacerbated by precarious conditions. The objective of this study was to analyze the socio-epidemiological situation. Materials and methods: A quantitative, descriptive and cross-sectional, evaluative study was carried out through the collection of data obtained from medical records of patients admitted to HUJBB between January 2005 and December 2011. Results and conclusion: A total of 2,915 patients diagnosed with Tuberculosis were found in the DAME database (Medical and Statistical Archive Division), with a predominance of males (67%), age range from 31 to 40 Years (25%). In the category composed of 50 patients with selected charts, we found the predominance of the age group of 21 to 30 years (30%), male (64%). Young male adults and using the basic scheme proved to be predominant in the study, that is, the largest portion of patients in full productive period, thus impacting the economy. Financial support or fellowship: HUJBB

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

EVALUATION OF THE USE OF ANTIMICROBIAL AGENTS IN DENTISTRY

CFSP082

BEATRIZ BARBOSA DA PAIXÃO1; NÁDIA REGINA BURIM ZUIM1; CAMILA STÉFANI ESTANCIAL FERNANDES2; RENATA LOPES RODRIGUES1; DANYELLE CRISTINE MARINI2. Fimi, Mogi Guaçu - SP - Brazil; 2Fimi, Mogi-Guacu - SP - Brazil.

Introduction and objective: It is common for dentists to prescribe medications to alleviate the painful effects of treatment. Antimicrobial agents are often used for prophylaxis or the cure of infectious processes. It is therefore necessary for dentists to know which microorganism is the cause of the infection and which antimicrobial agent to prescribe, since an incorrect prescription can lead to the emergence of resistant microorganisms. The aim of the present study was to evaluate the use of antimicrobial agents in dentistry in light of current legislation as well as determine whether patients were duly advised and aware of the medications employed. Materials and methods: This study was conducted at a dental clinic in the municipality of Mogi Guaçu in the state of São Paulo, Brazil, in May and June 2016 following approval from the local ethics committee (certificate number: 56183516.7.0000.5679). A questionnaire with open-ended and close-ended questions was used to collect the data. Results and conclusion: One hundred volunteers participated in the study. The female sex accounted for 53% of the sample. All participants reported having taken antimicrobial agents during dental treatment, the most frequent of which was amoxicillin (59%). The patients demonstrated satisfactory knowledge regarding the medications used, especially antimicrobial agents, and also understood the conditions that required the use of the medications. Thus, one may conclude that the dentists generally use antimicrobial agents (either alone or in combinations) for the pre-treatment and post-treatment of patients to alleviate pain and treat possible inflammation stemming from a given condition. Dentists also carefully inform each patient regarding the need to take antimicrobial agents and care related to the administration of such agents to ensure effective treatment. CFSP083 FIRST RESULTS OF A CLINICAL PHARMACY SERVICE AT A PEDIATRIC TERTIARY UNIVERSITY HOSPITAL IN BRAZIL MARCUS VINÍCIUS TERASHIMA DE PINHO; HELGA NAMIE FERREIRA MURAKAMI; DEISY ZAMA ASANOME; KATIA KAZUMI NAKADA; LUCIANA TOKIE KAWAI; SILVIA AKEMI SATO; PAULA FERREIRA CASTRO. Instituto da Criança, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Sao Paulo - SP - Brazil. Introduction and objective: The high complexity of care in a tertiary pediatric hospital leads to complex drug regimens; consequently, such patients are at high risk of developing drug-related problems. The activities developed by the clinical pharmacist play a key role in promoting rational drug use. In order to improve patient safety, we have implemented a clinical pharmacy service in all critical care and oncology units at the Instituto da Criança, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo (ICr HC-FMUSP). The aim of this study is to quantify and qualify interventions made by the clinical pharmacy service at a tertiary pediatric hospital in São Paulo, Brazil, in the first semester of 2017. Materials and methods: All interventions and services provided by clinical pharmacists were documented from January 1st, 2017 to June 30th, 2017. Results and conclusion: During this period, a total of 4026 prescriptions were analyzed, and 663 (16%) required at least one intervention, resulting in 1042 interventions. Interventions were related to posology (25%), information about drugs (12%), recommendation of inclusion, suspension or substitution of drugs on the prescription (11%, 9% and 4%), methods of drug administration (6%) and prescriptions in disagreement with institutional protocols (6%). Recommendations of drug treatment monitoring, changes in the pharmaceutical form or administration route and information on incompatibility of infused drugs were also interventions made during this period of time. The rate of acceptance of the interventions was 86% (893). Conclusion: The implementation of a clinical pharmacy service can positively impact patient care, improving both the quality of the pharmacotherapy and patient safety. The high acceptance rate of pharmaceutical interventions reflects a good relationship between the clinical pharmacists and the multidisciplinary care team.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP084 FREQUENCY AND FACTORS ASSOCIATED WITH VAGINAL INFECTIONS OF GYPSY WOMEN IN A CAMP IN THE CITY OF PINDORETAMA-CEARÁ CONCEIÇÃO MOREIRA NEPOMUCENO1; JANAINA LOPES DE MELO2; NAIRA LIMA SILVA2; TICIANA PRACIANO PEREIRA3; ANDRÉA BESSA TEIXEIRA3. Graduate in Pharmacy from Faculdade Metropolitana da Grande Fortaleza - Fametro, Fortaleza - CE - Brazil; 2Undergraduate Student in Pharmacy at Faculdade Metropolitana da Grande de Fortaleza - Fametro, Fortaleza - CE - Brazil; 3Professor of Faculty of Pharmacy at Faculdade Metropolitana da Grande Fortaleza - Fametro, Fortaleza - CE - Brazil. 1

Introduction and objective: Sexually transmitted infections are frequent, have multiple etiologies and clinical presentations, and affect a person’s quality of life and personal, familial, and social relationships. Another concern for health departments relates to cervical cancer, which is a sizable public health concern in Brazil. Societies have demonstrated difficulties in the integration of individuals with Gypsy heritage, with healthcare professionals being distanced from this cultural minority, hampering the provision of values, practices, and lifestyles that aid these professionals in specialized care. The objective of the present study was to determine the rate of vaginal infection in Gypsy women from a camp in the city of Pindoretama and evaluate factors that contribute to the prevalence of these infections. Materials and methods: A cross-sectional observational study was carried out, with detailed descriptions of the quantitative approach, utilizing a questionnaire presented to 50 Gypsy women. Results and conclusion: The results indicated that the predominant ages of the participants were between 17 and 25 (36%), and 26 and 35 (36%). Concerning the state of vaginal infection condition, 28% of the Gypsy women presented some level of vaginal infection. Regarding vaginal discomfort, the percentage of Gypsy women that had experienced it in some form was 64%, which represents more than half of the total number of participants. With respect to contraceptive methods, oral birth control was the most commonly mentioned (34%). Aside from these results, it is important to stress the troubling data indicating that 71% of these women confirmed the use of certain antibiotics without a prescription, usually prescribed before a Pap test. In this context, and in addition to access to quality healthcare services, education and prevention programs that involve a greater participation of healthcare professionals are necessary. Moreover, it is important to emphasize the role of the pharmacist within this multidisciplinary practice, because beyond being responsible for the administration of Pap tests and the microbiological identification of, and sensitivity to, antimicrobials, they are able to provide guidance relating to the correct usage of antibiotics. CFSP085 GARDENING, CULTIVATION AND MANIPULATION OF MEDICINAL PLANT SPECIES IN THE PENITENTIARY OF AMERICANA (CRA III) MUNICIPALITY OF SANTA ISABEL - PA. MYLENA CORREA SILVA; FLÁVIA MARTINS PACHECO FERREIRA; WEILLA PATRÍCIA CORDEIRO SILVA; ROSIENE SANTA ROSA ALCOFORADO; DANILA TERESA VALERIANO ALVES*; CHRISTIAN NERI LAMEIRA*. Faculdade Integrada Brasil Amazônia(Fibra), Belém - PA - Brazil. Introduction and objective: Live pharmacy aims to implement the cultivation of medicinal plants, to produce herbal medicines and promote the valuation of popular culture. Objective: To install cultivation of medicinal plants in the prison community promoting quality of life, through the cultivation and correct use of medicinal plants. Materials and methods: A 1m2 plant bed was made, with 50% shadow, 1m2 plant bed in open sky and pits of 50cm x 50cm, fertilized with organic compound in the proportion of 5 kg (bed) and 1 kg (pits). The species were chosen according to the prevalence of the main diseases that occurred in the penitentiary. The plant seedlings produced came from stakes and seeds obtained from the horticulture of EMBRAPA with maintenance of fertilization once a year. Results and conclusion: Forty species were cultivated, with all showing good development. The prisoners had manipulation training and from the cultivate plants in the garden the following were produced: Syrup, ointment, shampoos, soap and anti-cellulite creams. The occurrence of pests and caterpillars in some species was observed in the less rainy season, and was controlled with bioactive product and a mixture of water, soap and diesel oil. The observed variability in the species initially attended the main diseases that occur in the prison community and relatives. This process caused changes in the conditions and life prospects of the population of the district of Americana and the neighboring community. Financial support or fellowship: Faculdade Integrada Brazil Amazonia.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP086 GENERIC DRUGS: POPULATIONAL RESEARCH ON KNOWLEDGE AND CORRELATION WITH SOCIODEMOGRAPHIC AND SOCIOECONOMIC ASPECTS FRANCINE ARISATI DESTRO 1; LARISSA SALLES 1; LARIZA MAZA 2; PEDRINA CAMARGO 1; TALIANE CASAGRANDE TEIXEIRA1; FÁTIMA HADDAD BARRACH1. Universidade Paulista, Bauru - SP - Brazil; 2Faculdade de Medicina - Unesp, Botucatu - SP - Brazil.

Introduction and objective: Law no. 9,787/99, in addition to regulating generic drugs in Brazil, determines the responsibility of the Ministry of Health to promote mechanisms that guarantee communication to the population, information and education about generic drugs. Even after almost two decades of implementing this law, there is still a lack of information in the population about these drugs. This study aimed to analyze the knowledge of individuals interviewed in the city of Botucatu/SP and relate it to the sociodemographic and socioeconomic characteristics of the population in question. Materials and methods: The study was conducted with 154 random individuals between March and May 2017 and data collection occurred after the interviewee agreed to participate in the study and signed the Informed Consent Term. At the end of the interview, an accompanying explanatory pamphlet on medication was provided with adequate guidance to the interviewee. Results and conclusion: The results evidenced the lack of information about generic drugs and of confidence in their interchangeability. It was also demonstrated the need for government enlightenment programs on generic drugs, with a greater focus on the population with low income, low levels of education, individuals with chronic diseases, women and health professionals. CFSP087 GROUP EDUCATION AND PHARMACIST FOLLOW-UP EFFECTS ON BLOOD GLUCOSE CONTROL AND ADHERENCE TO TREATMENT IN TYPE 2 DIABETES PATIENTS ROBERT ANDERSSON FIRMIANO NICÁCIO1; ANNA KARLA CÉSAR LEANDRO2. Núcleo de Apoio À Saúde da Família (Nasf), Arapiraca - AL - Brazil; 2Ufal, Maceió - AL - Brazil.

Introduction and objective: It is estimated that more than 60% of patients with diabetes do not meet their glycemic goals and that only 12% achieve controlled BP and LDL-C less than 100 mg/dL. Pharmacist involvement in initiating and adjusting insulin regimens brings benefits to patient’s health and to health promotion. In this study, we aimed to assess the effects of an intervention bases of group education and pharmacist follow-up in outpatients using insulin. Materials and methods: We carried out an open-label trial, including insulin users attended at Basic Health Units (BHU) of Sítio Carrasco, Vila São José, Brisa do Lago and Cacimbas. for the family health strategy program to assess an intervention based on behavioral therapeutic groups and pharmacist follow up. Data were collected through questionnaires. Results and conclusion: The mean age of the 40 patients included was 62.7 years, most were female (75%). Approximately 32.5% of these individuals practiced some type of physical activity regularly. 42.5% were accompanied by nutritionist but only 32.5% followed the prescribed diet. Difficulty with the use of the insulin was reported by 72.5% of the users, the main complaint was pain at the moment of application (47.5%). When asked if this therapy had changed something in their everyday lives, 45% of the patients reported improvement in the quality of life. In respect to the laboratory tests carried out prior to and after the groups, it was observed that 40% of the patients had a decrease in the levels of systolic blood pressure and 75% in the blood glucose levels, 72.5% had total cholesterol levels reduced and 57.5% achieved target triglycerides levels. In relation to glycated hemoglobin, a decrease of 67.5% was observed when compared to initial values. On the basis of these results, we concluded that the formation of the therapeutic groups focused on insulin therapy provided significant improvement in the laboratory parameters evaluated prior to and after the intervention.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP088 HEALTH SCREENING FOR HYPERTENSIVE AND/OR DIABETIC PATIENTS IN THE PREVENTION OF CHRONIC RENAL DISEASE FLÁVIA MARTINS PACHECO FERREIRA; MYLENA CORREA SILVA; WEILLA PATRÍCIA CORDEIRO SILVA; ANDREZA SOUZA MIRANDA; CHRISTIAN NERI LAMEIRA*; DANILA TERESA VALERIANO ALVES*. Faculdade Integrada Brasil Amazônia-Fibra, Belém - PA - Brazil. Introduction and objective: Chronic Kidney Disease (CKD) is considered to be a major public health problem. The most relevant causes are chronic non-transmissible diseases that present higher prevalence in the country, having as main factors: Hypertension and Diabetes Melittus. One of the main factors that cause these diseases is directly related with society’s living habits. The objective of this study is the health screening of hypertensive and/or diabetic patients, verifying the influence of age and sex, in relation to a possible chance of developing Chronic Kidney Disease. Materials and methods: The present study is an applied, prospective, descriptive and quantitative approach, based on and adapted from the Dader Method, in order to realize the health screening of hypertensive and/or diabetic patients who participated in health actions for the prevention of CKD. To extract the information, the record of clinical parameters of CKD was applied to 120 hypertensive and/or diabetic patients (73 women and 47 men), volunteers, residents of the city of Belém do Pará. Results and conclusion: The results indicated that 92 patients (76.6% of the total) presented a 20% risk of developing CKD, especially in women (83.5% of the total number of women), with prevalence in patients aged between 50 and 59 years old (47.5%) with hypertension and associated diabetes. In male patients, the most prevalent age group was 60 to 69 years (58.0%). Early diagnosis of CKD helps in treatment of the disease. The pharmacist, with his diverse clinical skills, can assist in promoting and raising awareness through health screening actions. Financial support or fellowship: Faculdade Integrada Brazil Amazonia CFSP089 HERBAL PLANTS MARKETED IN BRAZIL FOR OSTEOARTHRITIS TREATMENT: SYSTEMATIC REVIEW CECILIA TORQUETI DE BARROS1; MARCUS TOLENTINO SILVA1; LAURA JÚLIA ESQUITINI LOPES1; TÂNIA REGINA FERREIRA2; LUCIANE CRUZ LOPES1; CRISTIANE DE CÁSSIA BERGAMASCHI1. Uniso, Sorocaba - SP - Brazil; 2Uniso.

Introduction and objective: Osteoarthritis (OA) affects 1% of the world population and is the most common cause of musculoskeletal impairment in the elderly. Herbal medicines are commonly used in Brazil to control the symptoms associated with OA, however, the effectiveness of most of these agents is still unclear. The aim of this study was to evaluate the efficacy and safety of 13 herbal medicines sold in Brazil for the treatment of OA. Materials and methods: This is a systematic review and meta-analysis of randomized controlled trials in adult patients with knee and/or hip OA treated with herbal plants: Harpagophytum procumbens, Uncaria tomentosa, Salix alba (financed by government), Curcuma longa (or C. domestica), Chenopodium ambrosioides, Cordia curassavica (or C. verbenacea), Zingiber officinale, Persea gratissima (or P. americana) (they are on the National List of Medicinal Plants of Interest to the Unified Health System) Boswellia serrata, Bowdichia virgilioides, Salix daphnoides, Salix purpurea and Uncaria guianensis compared with placebo or active controls. The search for eligible studies was conducted in electronic databases: CENTRAL; MEDLINE; EMBASE; CINAHL; Web of Science; Health Star; AMED, LILACS; CAB abstracts, clinical trial.gov, WHO Trial Register and Brazilian database of theses of CAPES. The primary outcomes were: pain, physical function, stiffness, edema, quality of life and the secondary outcomes: adverse events, satisfaction with the treatment, rescue medication consumption and change in the structure of the linkage. Results and conclusion: Sixteen studies were included (n= 1,741 patients) in SR and 9 studies were included in the meta-analysis corresponding to 6 of the 13 herbal medicines studied: B. serrata (n=2), C. longa (n=3), H. procumbens (n=1), S. daphnoides (n=3), U. guianensis (n=2) and Z. officinale (n=5). B. Serrata was more effective than placebo and valdecoxib for improvement of pain and physical function. No difference was observed for H. procumbens, C. longa and U. guianensis compared to control. Z. officinalle showed improvement of pain over placebo. Conclusion: The evidence was insufficient to support the effective and safe use of these herbal medicines, since the quality of evidence of studies was low. This study guides managers of the Brazilian public health system and prescribers in decision making regarding the use of these herbal medicines for OA. Financial support or fellowship: This project is funded by governmental Program Graduate Education Institutions-PROSUPCAPES/UNISO

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP090 HOME PHARMACY AND THE DISPOSAL OF MEDICATIONS IN THE MUNICIPALITY OF AGUAÍ, STATE OF SÃO PAULO, BRAZIL RUBIANNE DA SILVA MELO1; NÁDIA REGINA BURIM ZUIM2; CAMILA STÉFANI ESTANCIAL FERNANDES1; RENATA LOPES RODRIGUES2; PAMELA SILVA PEREIRA2; DANYELLE CRISTINE MARINI1. Fimi, Mogi Guaçu - Brazil; 2Fimi, Mogi Guaçu - SP - Brazil.

Introduction and objective: The majority of the Brazilian population keeps medications in the home, which can accumulate in the medicine cabinet, forming a home pharmacy that may lead individuals to self-medicate. The practice of self-medication is considered one of the causes of the irrational use of medicines. The indiscriminate use of medications, especially non-prescribed medications, can have serious consequences, such as poisoning. Moreover, having a home pharmacy is often associated with inadequate storage and disposal, which can cause environmental problems and place the health of the population at risk. The aim of the present study was to analyze the practices of having a home pharmacy and discarding medications in the population of the municipality of Aguaí, Brazil. Materials and methods: A descriptive, cross-sectional study was conducted following approval from the local ethics committee (certificate number: 43346815.3.0000.5679). A questionnaire was administered to 100 volunteers residing in the municipality of Aguaí in the state of São Paulo, Brazil. The volunteers were selected randomly using a probabilistic sampling method. Results and conclusion: The frequency of home pharmacies was high (95%). The individuals who most used these medications had a complete high school education (34%) and household income one to three times the Brazilian monthly minimum wage (43%). The most common medications were analgesics (24.4%). A total of 47% of the participants reported storing medications in cabinets and drawers and 52% reported discarding medications in the common trash. For the home pharmacy to be beneficial, one must consider the care necessary in terms of acquiring, storing, taking, and discarding medications. Patients should seek the advice of physicians and pharmacists to ensure the rational use of medicines.

HOME PHARMACY: EVALUATION OF BEDRIDDEN PATIENTS’ MEDICATIONS

CFSP091

CASSIELA ROMAN; EVELISE DE SOUZA STRECK; LUCIANE KOPITTKE. Grupo Hospitalar Conceição, Porto Alegre - RS - Brazil. Introduction and objective: The home pharmacy may consist of a variety of medications: used in current treatment for acute or chronic diseases; leftovers from previous treatments; eventual use, such as self-medication; out of date or of doubtful validity. The aim of this study was to evaluate the home pharmacy of bedridden patients in the territory of a Family Health Strategy (FHS), in a city in the South of Brazil, who have a responsible caregiver. Materials and methods: Data on the home pharmacy of bedridden patients were collected at home visits through a semi-structured interview with caregivers. Results and conclusion: Of the 12 bedridden patients assisted by the FHS, nine caregivers from different patients agreed to participate in the study. In the residences visited, medicines are stored in the living room (44%) and in the bedroom (33%) free of exposure to light, heat, moisture or children. Concerning disposal of medicines, 67% handed them over to FHS, which shows attention to the appropriate disposal. Of the 43 medicines found, three were out of date and in two households it was reported that there were medicines for self-medication in the home pharmacy, demonstrating caution against this practice. The mean number of medications prescribed was 6.2 medicines/patient, the most prescribed being Enalapril (55%), Simvastatin (55%), Acetylsalicylic Acid (44%), Paracetamol (33%) and Omeprazole (33%). Regarding the knowledge about medicines used by patients, 32% of caregivers state that they know the purpose of the medication. Most are unaware of the prescribed dose (88%), how to use it (88%) and administration time (84%), as well as interaction with other medications or foods. It is noticed that the performance of the pharmacist with the health team of the FHT can assist in the coordination of the care and safety of bedridden patients, through guidance on the medicines used. Financial support or fellowship: Ministério da Saúde

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

HOMEOPATHIC MATERIA MEDICA: STUDY OF PATHOGENESIS OF ARNICA MONTANA L.

CFSP092

CAMILA SEIXAS DE OLIVEIRA; HELENA ONISHI FERRAZ. Universidade Federal de SĂŁo Paulo, SĂŁo Paulo - SP - Brazil. Introduction and objective: Homeopathy, a science of cure by similarity principle, was founded in 1796 by Samuel Hahnemann, who during his studies found that substances capable of generating a group of symptoms in a healthy individual were able to cure patients with similar symptoms. The set of symptoms triggered in a healthy organism, when experiencing substances, was denominated pathogenesis in homeopathy. The use of Arnica montana L. was recommended by Hahnemann, who initially indicated the use of the whole plant, but the discovery of eggs of a parasitic fly in the flowers led to the use of only the root. Thus, the main focus of this work was to study the species Arnica Montana L. to discover the chemical substances and the pathogenesis described according to the whole plant or parts thereof. Materials and methods: Bibliographical survey in prominent journals, through the use of the following databases: PubMed, SciELO, Science Direct, BV Unifesp, and Google Academic, without restriction as to date or locality. Main keywords: homeopathy, Arnica montana L., Hahnemann, high dilutions, pathogenetic experimentation, medical matters. Books, academic theses, official compendia, World Health Organization (WHO) documents and guidelines from regulatory bodies were also consulted. Results and conclusion: The pathogenesis of the use of the whole plant is widely found in medical matters; however, the pathogenesis of root use is not present in them. The literature shows that both the aerial and underground parts of A. montana have secretory structures, but the chemical group is different and helenalin, which is mainly responsible for pharmacological effects (such as anti-inflammatory activity, cicatrization and reduction of edema), is found in the aerial parts. Taking into consideration that the treatment of patients is performed using both the whole Arnica montana L. plant and only its roots, it is important to note that use of only roots would probably lead to a different set of substances present in the mother tincture and consequently, would alter its pathogenesis, influencing the outcome of the treatment. CFSP093 IDENTIFICATION OF MEDICINAL INTERACTIONS IN PATIENTS WITH PSEUDO-RESISTANT HYPERTENSION TAMMYRYS NUTELS; ALFREDO DIAS DE OLIVEIRA FILHO; SABRINA JOANY FELIZARDO NEVES. Universidade Federal de Alagoas, Maceio - AL - Brazil. Introduction and objective: Hypertension (HA) is a highly prevalent clinical condition with a high negative social impact. The failure of treatment is related to factors such as inadequate blood pressure (BP), white coat effect, low adherence, inadequate therapy and occurrence of drug interaction (DI) which lead to Pseudoresistant Arterial Hypertension (PHTN). This study aims to investigate the prevalence of drug interactions among people with PHTN. Materials and methods: Measurements of BP, application of forms for sociodemographic data collection, use of medications and therapeutic adherence were performed. Potential DIs were identified through the Micromedex Healthcare Series software and manifests, by clinical data and confirmation with the aid of supporting literature. Results and conclusion: 233 patients were included in the study. It was observed that 124 patients had a prevalence of 53.2% of potential drug interactions (PDI). The most frequent PDI occurred with medications used for self-medication Financial support or fellowship: FAPEAL

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP094 IDENTIFYING THE PREVALENCE OF QUALITY NOTIFIED MEDICATION ERRORS IN A PEDIATRIC HOSPITALTHE IMPORTANCE OF THE INVOLVEMENT OF THE MULTIPROFESSIONAL TEAM GABRIELLA CANDIDO DE FREITAS LANDIM; ANAHI AYALLA CÉSAR BASSI. Hospital Infantil Sabará, São Paulo - SP - Brazil. Introduction and objective: The occurrence of medication errors is a common topic amongst hospital administrators around the world. Nowadays, health facilities and institutions tend to take preventive measures to reduce adverse incidents. We have identified all the medication mistakes that occurred between January 2015 and March 2016 in a pediatric hospital in São Paulo indicated by Quality Control in order to detect the prevalence of the reported errors. Materials and methods: The chosen method was non-experimental research, based on description and analysis of 119 error notification reports by the multi professional team. Results and conclusion: The data showed that 61.3% of the notifications were unfavorable events, from which 19.2% resulted in some kind of harm to the patient. Also, 23.3% of the events were related to medication described as high alert medication. The stage in which most of the mistakes happened was the route of administration, followed by mistakes related to the compliance of drug schedule. The most frequent mistakes are drug dosage error, mainly overdoses. In conclusion, the participation of top management and area manager personnel is fundamental so that a high quality and security culture is established in consolidated and non-punitive terms. Financial support or fellowship: Hospital Infantil Sabará CFSP095 IMPLEMENTATION OF PHARMACEUTICAL PROCEDURES IN THE UNIFIED HEALTH SYSTEM (SUS): FOCUSING ON MEDICATION ADHERENCE FOR CHRONIC CARE. JULIO EDUARDO PEREIRA DE SOUZA1; MARINA TIEMI SHIO2. Associação Saúde da Família, São Paulo - SP - Brazil; 2Universidade Santo Amaro, São Paulo - SP - Brazil.

Introduction and objective: Poor adherence to pharmacotherapy is a major problem for management of chronic non-communicable diseases (CNCDs), which increases hospital admissions and emergency services for patients with CNCDs, raising the SUS’s economic expenditure. The São Paulo Municipal Health Department approved the ordinance number 1.918/2016 that adopts pharmaceutical care in the primary and tertiary health care network. Herein, we evaluated the view of those pharmacists regarding the most common reasons for nonadherence to medication, as well as, the role of the pharmacist in the Family Health Teams to implement interventions and to improve therapeutic outcomes. Materials and methods: We used a simple questionnaire and analyzed the perception of 32 pharmacists from the multidisciplinary Family Health Teams located at Capela do Socorro and Parelheiros – in the south of São Paulo city. Results and conclusion: The following reasons for patient’s non-adherence to drug therapy have been identified: discontinuation (37.5%) or omission (25%) of use of the medicines, error in frequency of administration (15.6%) or in dose administration (12.5%) or in the treatment duration (3.1%), and undue continuation (6.3%). The interventions comprised guidance for patients concerning drug storage (75%), access to medicines (65.6%), specific treatment (62.5%), general treatment (59.4%), nonpharmacological actions (56.3%), self-monitoring (46.9%), specific and general health condition (37, 5%). Pharmacists judged themselves capable all (62.5%) or most of the time (31.3%) or not (6.3%) capable of intervening. Improvement of the treatment adherence was by self-reporting (56.3%), reported during team’ meetings (25%) or by the Community Health Worker (9.4%) or by the Physician (3.1%). This preliminary study suggest that the pharmaceutical interventions can potentially improve the adherence to pharmacotherapy by patients with CNCDs. The implementation of the ordinance number 1918/2016 will be important to the clinical and therapeutic follow up of those patients.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP096 IMPLEMENTATION OF THE FARMACIA VIVA PROJECT IN CENTRO UNIVERSITÁRIO DE JAGUARIÚNA ALINE XAVIER FURLANETO; IARA APARECIDA BOIAGO; JARDELIANE PEREIRA NUNES; LARISSA DAIANE VILLANOVA ANECHINI; RAQUEL MEDEIROS PEREIRA; ANDRÉ LISBOA RENNÓ; RENATA CAVALCANTI CARNEVALE. Centro Universitário de Jaguariúna, Jaguariúna - SP - Brazil. Introduction and objective: The use of medicinal herbs has been passed on through the time and is, even in the recent days, widely used by people worldwide. However, knowledge about medicinal herbs and their popular use is being lost. The Farmacia Viva project aims to rescue traditional and popular knowledge of medicinal herbs and promote their rational use. Materials and methods: The project was approved by Centro Universitário de Jaguariúna in 2017. It was constructed in a suitable place in Interclinicas, Jaguariúna – SP, which is a primary care unit. There are 29 different medicinal herbs cultivated, such as Curcuma longa L., Plectranthus barbatus Andrews, Cymbopogon winterianus Jowitt, Aloe vera L., Melissa officinalis L. and Lippia alba (Mill) N. E. Brown. Results and conclusion: Many activities are being performed such as research with population, approved by the Institutional Review Board (IRB) of Centro Universitário de Jaguariúna, to establish the medicinal herbs most used by them; use of the herbs in classes of Pharmacy graduation course; leaves for microscopic observation; development of a guide of medicinal herbs and a blog with information and clarification of doubts about them. Also, it is a field for trainee and student research. Activities such as medicinal herb workshops, lectures and courses are being planned. The community will also receive the herb in natura, and explained how to use it, indications and cultivation. In the future, the objective will also be to provide vegetal drugs and eventually herbal medicines to the population. The Project proved to be well accepted by the local community, by both doctors and patients. Its contribution was shown to be positive for medicinal herb divulgation and for academic knowledge extension, also being seen not only as research field but as therapeutic alternative. Financial support or fellowship: Centro Universitário de Jaguariúna (UNIFAJ) CFSP097 IN VITRO STUDY OF PROLIFERATIVE FIBROBLAST ACTIVITY IN HUMANS SUBMITTED TO HOMEOPATHIC TREATMENT WITH ZINCUM METALLICUM AND CALENDULA OFFICINALIS IN ULTRA DILUTIONS VICTOR CAVALARO1; DANIEL GONSALES SPINDOLA2; VALERIA MARIA DE SOUZA ANTUNES1; CLAUDIA BINCOLETTO TRINDADE3; CARLOS ROCHA DE OLIVEIRA2. University Anhembi Morumbi, São Paulo - SP - Brazil; 2Federal University of São Paulo, São Paulo - SP - Brazil; 3University of São Paulo, São Paulo - SP - Brazil. 1

Introduction and objective: The cicatrization process is immensely complex, since it is not a linear but dynamic event involving several cellular and biochemical events for the repair of lesions, directly implying an increase in fibroblastic activity. In this sense, homeopathy has advanced in studies for cellular stimulation and protection. Objective: Verify the stimulation of the healing process, the homeopathic pillar of similute was used the Zincum metallicum 6CH and Calendula officinalis 6CH applied independently in different concentrations in fibroblast cultures to determine the increase in proliferative activity using techniques such as IC50, MTT, flow cytometry and Quantification of collagen. Materials and methods: Human fibroblasts of the CCD-1072 Sk treated with the percentages of crescent application of 1% to 5% in relation to the volume of the culture of Zinc metallicum 6CH and Calendula officinalis 6CH. The cytotoxicity (IC50) evaluation was performed by the trypan blue technique and with the reduction of MTT to formazan. The tests were finalized with the realization of the Sirus Red technique and flow cytometry. All results was treat with ANOVA with Tukey correction and T test. Results and conclusion: According to the statistically significant results, there was no indication of IC 50 for the highest dose in the tests for either homeopathic drug, both groups maintained percentage of viable cells and proliferation of cells were observed in both treated cultures when compared to the control. The evaluation of MTT and Sirus Red in treated CCD-1072SK indicated stimulus in oxidative metabolism and also in collagen biosynthesis for both drugs. Flow cytometry indicated greater relevance in phase S-G2 of the M phase for the group treated with C. officinalis. It is concluded that there are possibilities for both drugs to stimulate the proliferation of fibroblasts, with Calendula being the most indicated in tested dose, thus suggesting its potential use in the cicatrizant treatment and by the stimulation of collagen production as well as dermocosmetics. Financial support or fellowship: PIBIC - University Anhembi Morumbi

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

INAPPROPRIATE PRESCRIBING FOR PSYCHIATRIC PATIENTS AT A PUBLIC HOSPITAL

CFSP098

LAURA JÚLIA ESQUITINI LOPES; MILLA DE PAULA; CRISTIANE DE CÁSSIA BERGAMASCHI; TÂNIA REGINA FERREIRA. Uniso, Sorocaba - SP - Brazil. Introduction and objective: Introduction and objectives: The last decade has seen an increase in the consumption of psychotropic drugs and, consequently, in the concern about the effectiveness and safety of these medicines. Therefore, checking the adequacy of the prescriptions of these drugs contributes to the rational use of these medicines. The aim of this study was to verify the adequacy of the use of medicines prescribed for psychiatric patients attended in a public hospital. Materials and methods: Materials and methods: Data was collected from all medical records of patients attended in the Psychiatry sector of “Conjunto Hospitalar de Sorocaba”, State of São Paulo, Brazil, from August 2015 to June 2016. The prescription was considered as appropriate if it had adequate indication, contraindication and absence of serious drug or contraindicated interactions, and a correct posology. This was verified according to information contained in the standard package leaflet obtained from the Anvisa website and the Drugdex® System. Results and conclusion: Results: The sample was of 171 patients, the majority being adults (81.3%) and women who mainly made use of diazepam and haloperidol and whose main diagnoses were schizophrenia and mood disorders. It was observed that 70.9% of prescriptions (287) were appropriate, but only 33.3% of patients (57) made appropriate use of medicines. Indication errors were present in 3.7% of the prescriptions, the majority being due to the use of sodium valproate in patients who had epilepsy. Around 23% of the prescriptions presented risk of serious drug interactions such as, for example, the association of haloperidol and chlorpromazine which can cause cardiotoxicity and prolongation of the QT interval. No posology error was observed. Conclusion: although most of the prescriptions of psychotropic drugs were adequate, it corresponded to a minority of patients. These results suggest the need for adaptation of these prescriptions aiming an effective and safe use of these medicines. Financial support or fellowship: Financial support or fellowship: Institutional Scholarship Program for Scientific Initiation. CFSP099 INFLUENCE OF ACCESSION IN CHRONOTHERAPY OF THE LOSARTAN DRUG - A CROSS-SECTIONAL STUDY ROSILEIDE ZEFERINO DA SILVA; ALFREDO DIAS DE OLIVEIRA FILHO; SABRINA JOANY FELIZARDO NEVES. Ufal, Maceió - AL - Brazil. Introduction and objective: Chronotherapy is an approach to increase the efficiency of pharmacotherapy. This is the administration of medications at times that are more effective and better tolerated. Antihypertensive classes showed significant differences in efficacy depending on the time of day of administration. However, as HAS is a lifestyle disease, therefore being multifactorial, the approach and therapy should also consider adopting a healthy lifestyle as well as adherence to therapy. This study aims to evaluate the impact of the use of chronotherapy of losartan in the control of pressure levels in patients with hypertension. Materials and methods: A cross-sectional study was carried out with hypertensive patients aged over 18, in monotherapy with losartan, treated at one Family Health Strategy Unit in Maceió (AL), through interviews and blood pressure measurements, during 2015. The following variables were investigated: gender, age, medications, dosage, time of use of antihypertensives, and systolic and diastolic pressures. The study was approved by the Ethics Committee in Research of Universidade Federal de Alagoas, certificate No. 19148713.1.0000.5013. Data were collected only after the informed consent had been signed by all patients. Results and conclusion: Of the 94 subjects included in this study, 64 (68.1%) were female, 24.5% used losartan in the morning and 75.5% in the evening. Patients taking losartan in the morning had 6.73 mmHg of SBP (p = 0.102) and 5.89 mmHg of DBP (p = 0.025) less than those who used at night. However, it is important to note that patients who used losartan at night had lower adhesion rates (19.7%) than those in the morning (43.5%) Financial support or fellowship: FAPEAL; CAPES

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP100 NTERVENTIONS OF CLINICAL PHARMACIST IN AN INTERMEDIARY UNIT CARE WITH FOCUS ON ELDERLY PATIENTS STÉPHANIE DE SOUZA COSTA VIANA1; TIAGO ARANTES2; SABRINA CORREA DA COSTA RIBEIRO2. Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo - Brazil; 2Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo - SP - Brazil. 1

Introduction and objective: The world is going through an epidemiological transition, with an increase in the elderly population and the challenges related to it. Pharmacokinetic and pharmacodynamic differences in the elderly group and the high number of medications in use by them justify the need of pharmaceutical care and clinical pharmacy in this group. Because of this, the objective of this study is to discuss the role of the clinical pharmacist in the care of this population, in a teaching hospital in São Paulo. Materials and methods: Pharmacotherapeutic accompaniment of critical patients aged 60 and over, admitted by the clinic medical team in an Intermediary Unit Care, was conducted for four months. Medical prescriptions were evaluated daily based on patients’ clinical conditions. These evaluations were made to identify opportunities to optimize the pharmacotherapy prescribed, with the following goals: contribute to the greater security of prescription, reduction of discomfort and stimulate the rational and correct use of medications. Results and conclusion: 386 prescriptions were evaluated and 212 pharmaceutical interventions were performed. 64.3 % of these interventions were accepted and changes were made to the prescriptions, 28.5% were not accepted and 7.2 % were accepted verbally but changes were not made. The orientations to dose adjustments, pharmacotherapeutic indications, reduction of the use of potentially inappropriate medications for the elderly, adjustment of prescription, suspension of unnecessary drugs, were some interventions made by the pharmacist. The significant number of interventions accepted by the health team shows that the clinical pharmacist has an important role on the multi-professional team especially when it is related to elderly care. CFSP101 KNOWLEDGE AND USE OF INDIA NUT (ALEURITES MOLUCCANA L.) BY THE POPULATION AND HEALTH PROFESSIONALS OF JAGUARIÚNA AND HOLAMBRA CITIES STEPHANIE OLIVEIRA CARVALHO1; KAROLINE GALLI DE FREITAS2; POLYANA DE OLIVEIRA IRICEVOLTO3; MICHELLE PEDROZA JORGE4; RENATA CAVALCANTI CARNEVALE4. Faculdade de Jaguariúna, Jaguariuna - SP - Brazil; 2Faculdade de Jaguariúna, Holambra - SP - Brazil; 3Faculdade de Jaguariúna, Jaguariúna - SP - Brazil; 4Faculdade de Jaguariúna, Campinas - SP - Brazil. 1

Introduction and objective: Aleurites moluccana L., popularly known as India Nut, is a plant, prohibited by National Health Surveillance Agency (ANVISA), that has been sold on the internet and popular markets to reduce weight due to its laxative properties. It has been used in an abusive way without orientation of a qualified health professional and is causing serious adverse reactions and even death. The objective of this study is to evaluate the knowledge and profile of use of Aleurites moluccana L., by the population and health professionals of Jaguariúna and Holambra cities. Materials and methods: It is cross-sectional research which included 34 participants: 12 from the general population and 22 health professionals from Jaguariúna and Holambra cities. All participants were submitted to an interview that aimed to identify the use, adverse effects and intoxications associated with Aleurites moluccana L. The research was approved by University Center of Jaguariuna (FAJ)’s Institutional Review Board (IRB). Results and conclusion: A total of 5 professionals (22.7%) interviewed indicated Aleurites moluccana L. for weight loss and cholesterol reduction. 11 people (92%) from the general population interviewed used Aleurites moluccana L. without a health professional’s orientation and 22 (64.7%) of the total interviewees did not know that there is no product containing Aleurites moluccana L. registered in Brazil and that its sale is prohibited by ANVISA. Interviews have not been finalized yet, as the objective is to include 60 people from the general population and 40 health professionals from Jaguariúna and Holambra cities. However, we can conclude so far that the irrational use of Aleurites moluccana L. is frequent both by health professionals, who indicate it even knowing its risks, and by the general population who do not know about its prohibition and who use it without the orientation of a health professional. Financial support or fellowship: University Center of Jaguariuna (FAJ).

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP102 KNOWLEDGE EVALUATION ON SANITARY LEGISLATION BY PHARMACISTS WORKING IN DRUGSTORES IN TWO MUNICIPALITIES IN THE INTERIOR OF SÃO PAULO/BRAZIL ARIANE DE PAULA BOLPETTI; FABRICIA HELENA SANTELLO. University Center of The Educational Foundation of Barretos/Unifeb, Barretos - SP - Brazil. Introduction and objective: Historically, the pharmacist moving away from care area, allowing others to assume their role, puts the population health at risk. Inadequate use of medicines has become a global public health problem, requiring the resumption of Pharmaceutical Care, and for this, the regulation of all marketing and dispensing activities was required. Pharmaceutical services were officially regulated by RDC 44/09. The aim of this study was to evaluate the knowledge about some aspects of current legislation of technical responsible person and/or substitutes in non-compounding pharmacies. Materials and methods: The data were performed with responsible pharmacists and/or substitutes in the municipalities of Colina/ SP and Barretos/SP, through the application of a questionnaire containing open and closed questions. Results and conclusion: The majority of respondents were women (72%), graduated from the University Center of the Educational Foundation of Barretos (UNIFEB) (78%), from private institutions (96%), with the curriculum divided between generalist (49%) and modalities (51%) and course conclusion between 1995 and 2015. The level of knowledge about legislation in the occupation area was considered good by 64% of professionals as well as by 66% regarding RDC 44/09. Of all interviewees, 62% declared that they provide pharmaceutical services, including home pharmaceutical care (16%) and biochemical physiological parameters (39%). In conclusion, some evaluated professionals presented shortcomings in the legislation and regulation of the profession, which may compromise the service quality to the patient. Financial support or fellowship: UNIFEB CFSP103 MEDICATION ADHERENCE AMONG HYPERTENSIVE PATIENTS WITH FREE ACCESS TO ANTIHYPERTENSIVE TREATMENT MIRELA QUIRINO DE ALMEIDA; ANDRESSA LEITE LEÃO; SABRINA JOANY FELIZARDO NEVES; ALFREDO DIAS DE OLIVEIRA FILHO. Ufal, Maceió - AL - Brazil. Introduction and objective: Medication cost is one of the main barriers to medication adherence. One of the attributes of the Brazilian Unified Health System is to guarantee free access to essential drugs. This study aimed to investigate the association between free access to antihypertensive drugs and medication adherence. Materials and methods: We conducted a cross-sectional study on hypertensive adult patients attended at public and private community pharmacies in Maceio, Brazil, between January 2011 and December 2014. Values of BP were obtained according to the Sixth Brazilian Guidelines for Hypertension. Adherence was determined using MMAS-8. Results and conclusion: Overall, 1,228 patients were included in the study and 55.7% had adherent behavior. Medication adherence rate was higher (60.8% vs. 53.7%) among patients that paid for their treatment compared to patients with free access to antihypertensive treatment (p = 0.026). Medication adherence was related to BP control in non-diabetic patients (p = 0.005); however, among patients with diabetes, this association was not observed (p = 0.099). Age (over 60 y.o.) (p = 0.007) and physical activity (p = 0.001) were the factors associated with a higher degree of adherence, while low level of education (p = 0.006) was associated with low medication adherence. Free access to medication was not associated with better medication adherence. However, among the low-income population, free access corresponded to the only access route to antihypertensive treatments. Financial support or fellowship: FAPEL

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP104 MEDICATION ADHERENCE OF TYPE 2 DIABETIC PATIENTS TREATED AT PUBLIC HEALTH SERVICE CENTERS WITHOUT MULTIDISCIPLINARY CARE. DANIELA QUADROS DE AZEVEDO. Santa Casa de Misericórdia de Belo Horizonte - Mg - Iep (Instituto de Ensino e Pesquisa)., Belo Horizonte - MG - Brazil. Introduction and objective: It is a descriptive and cross-sectional study conducted at the Municipal Center of Specialized Care, Sabará – MG, in 2015. Objective: assess medication adherence of type 2 diabetic patients treated at public health service centers without multidisciplinary care. The study population consisted of 48 type 2 diabetic patients who met the inclusion and exclusion criteria. Materials and methods: For data collection, a questionnaire elaborated by Faria (2008) about adherence to medication therapy by diabetic patients was utilized. Following this, the Measurement of Treatment Adherence - MTA test was done. Statistical analysis included the chi-square test, and alternatively Fisher’s test was applied. Comparisons of continuous variables were made through analysis of variance and the Student-t test. Results and conclusion: In the correlation analysis between adherence and socio-demographic characteristics, there was a statistically significant correlation with the female gender. There was no significant difference in adherence between patients who reported difficulty in obtaining medicines and those with better access. Also, no significant difference was found in the distribution of adherence when the oral anti-diabetes medication is associated with insulin therapy. There was no significant difference in the distribution of adherence in relation to the presence or absence of side effects. There was also no significant difference in the prevalence of adherence for the following variables investigated: information received by the patient about the DM and prescribed drugs. The patients’ diabetes time was also compared between the groups of adherence and non-adherence to treatment. With regard to diabetic patients’ adherence to drug treatment for DM, it was found that of the 48 patients (100%) 87.5% showed adherence. Conclusion: the prevalence of adherence obtained is above that found in the literature. However, this fact does not exclude the urgent need to recognize the importance of measuring the adherence of diabetic patients to pharmacotherapy, by health professionals, in the presence of a poor glycemic control and a failure in the therapeutic regimen. CFSP105 MEDICATION INCOMPATIBILITY INCIDENCE IN NEONATAL INTENSIVE CARE UNIT, A PREVENTIVE STRATEGY STUDY. ALESSANDRA COUTO BOAVA1; FABIANA DA SILVA FISNACK1; FLAVIA RIGOS SALGUEIRO2; CRISTIANE ELOÍSA VENÂNCIO GUEDES3; ANDRÉIA CRISTINA ZAGO DA SILVA1. Universidade São Francisco, Bragança Paulista - SP - Brazil; 2Universidade Santa Cecília, Santos - SP - Brazil; 3Hospital Universitário São Francisco, Bragança Paulista - SP - Brazil.

Introduction and objective: Neonatal intensive care unit (NICU) inpatients receive a large amount of medications via parenteral. Often, two or more drugs are administered simultaneously through the same lumen and the incompatibility physical-chemical risk is substantial. The precipitate formation is the clinical risk of greatest relevance, it can cause patients decease by diffuse microvascular pulmonary embolism formation. The study aims to discover the incidence of drug incompatibility in NICU at the Sao Francisco University Hospital. Materials and methods: The inclusion criteria apply to all NICU newborn inpatients who were submitted to intravenous therapy with two or more components. The data gathering was performed by completing the, “Form of choice of access via”, for three months. For incompatibility evaluation, the following tools were used: Blackbook® 2016, King Guide® 2017. The statistical analysis was performed by Excel program. Results and conclusion: Of the 156 prescriptions analyzed, 48% presented medicinal incompatibilities, with 46.6% showing two or more incompatibilities in the same prescription, 48% are associated with parenteral nutrition interaction and 68% are associated with antibiotics. The specific drugs administration and infusion standardized structured in lumens of central venous catheters is an important step to prevent the relevant incompatibility reactions, it makes the clinical pharmacist work indispensable in sectors such as NICU.

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CFSP106 MEDICATION RECONCILIATION BY A PHARMACIST AT ADMISSION PATIENTS ATTENDED IN URGENCY AND EMERGENCY DEPARTMENT IN GENERAL HOSPITAL OF SÃO PAULO VANESSA ZACARIAS TRINDADE1; MARCIA EUGENIA DEL LLANO ARCHONDO1; ADILSON SILVA MARQUES1; ALEXANDRE MASSAO SUGAWARA2. Universidade de Santo Amaro, São Paulo - SP - Brazil; 2Universidade Anhembi Morumbi, São Paulo - SP - Brazil.

Introduction and objective: Medication reconciliation is a formal process primarily characterized by the Clinical Pharmacist initiative to obtain a complete and accurate list of patient’s current home medications comparing the physician’s admission, transfer or discharge orders to that list. It permits the detection of prescription discrepancies and errors that can negatively affect the evolution of the patient’s treatment, offer a plan of care to the physician and verify its acceptance. The aim of this study was to evaluate patient pre-admission medications and post admission prescriptions verifying discrepancies and errors of prescriptions and the impact of reconciliation on patients admitted to the urgency and emergency department. Materials and methods: Materials and methods: Eighty-seven patients participated in this study. Patient’s home medications were compared with admission, transfer or discharge prescriptions. When non-intentional discrepancies, medication errors, were found the pharmacist communicated with the physician. Conciliation was realized when intervention was accepted. Results and conclusion: Results and conclusion: There were evaluated 174 prescriptions, with 229 prescribed medications. Discrepancies were present in 62% medications. Intentional discrepancies were 23% and 76.7% were medication errors. 97% of pharmaceutical interventions were realized and the medical team accepted 65% of them. Accepted interventions reduced the impact of prescription errors between care levels to 27.5%. Financial support or fellowship: Multiprofessional residence Ministry of Health and Ministry of Education

MEDICATIONS UNDER SPECIAL CONTROL PRESCRIBED BY NON-MEDICAL PROFESSIONALS

CFSP107

IONARA VIEIRA ROCHA DA MOTA1; REBECA SANTOS CARDOSO1; NARA JACQUELINE SOUZA DOS SANTOS1; PAULO HENRIQUE RIBEIRO FERNANDES ALMEIDA2; LUCAS BRASILEIRO LEMOS1; GISELE DA SILVEIRA LEMOS3. Universidade Estadual do Sudoeste da Bahia, Jequié - BA - Brazil; 2Universidade Federal de Minas Gerais, Belo Horizonte MG - Brazil; 3Universidade Estadual do Sudoeste da Bahia, Jequié - BA - Brazil. 1

Introduction and objective: The proper prescription of drugs under special control by legally qualified non-medical professionals favors quality in dispensing and provides conditions for rational and non-abusive use of these substances. The objective of this study was to describe the profile of drugs under special control prescribed by non-medical professionals. Materials and methods: A cross-sectional, descriptive study based on documentary analysis of special control recipes and notifications of dental and veterinary revenues in 25 months (June 01, 2014 to June 30, 2016) filed at a community pharmacy in the municipality of Jequié/BA. Results and conclusion: A total of 235 prescriptions were analyzed, of which 67.7% (159) were prescribed by dental surgeons and 32.3% (76) by veterinarians. In the prescriptions of the veterinarians, a predominance of antiepileptic drugs (phenobarbital) with a percentage of 75% (57) and hypnotics/sedatives (zolpidem) with a percentage of 6.6% (5) were found. In the prescriptions of dental surgeons the most prescribed medication was paracetamol + associations, being the association with codeine 69.8%, followed by antiepileptic drugs with 15.5, with pregabalin being 10.1% of prescriptions. In this work it was possible to describe the drugs most prescribed by dentists and veterinary surgeons. In addition, it is important to note that in Brazil, there are still no legislation that defines drugs for a rational therapeutic arsenal for the dental and veterinary profession. Financial support or fellowship: UESB; FAPESB

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CFSP108 MEDICINAL PLANT USAGE AND TREATMENT ADHERENCE BY HYPERTENSIVE PATIENTS IN THE MUNICIPALITY OF SANTARÉM-PA ILVIA SILVA GOMES1; ANDERSON GONÇALVES DOS SANTOS PEREIRA2; ELAINE MENEZES ROSSI2; JAMILLY DOS SANTOS BEZERRA2; MAIRA RAYANE PEREIRA BRAGA2; MATHEUS MALVEIRA VAZ2; WILSON SABINO2. Universidade Federal do Oeste do Pará - Ufopa, Santarém - PA - Brazil; 2. ??????

Introduction and objective: Nonadherence to treatment is one of the main reasons why it is difficult to control arterial hypertension. It may be influenced by several factors: cultural, social or economic ones. The objective of this study was to analyze the relationship between medicinal treatment adherence and medicinal plants usage by hypertensive patients. Materials and methods: 62 individuals registered in the Hiperdia program of two Basic Health Units (UBS) in the Municipality of Santarém-Pará, one in the urban area and the other by the riverside, participated in the research. Sociodemographic data from these patients were collected using a questionnaire, and the usage of medicinal plants was investigated. In order to check the adherence level the eight-item Morisky test was used. The analysis of the variables was performed using the Statistical Package for the Social Sciences program (SPSS- version 20). Results and conclusion: Of the 62 patients, 45.2% were from the riverside UBS, of which 78% did not adhere to the treatment and made use of medicinal plants (p=0.02). Those who were interviewed in the urban UBS represent 58.4% (p>0.05) of whom 58.9% did not adhere to the treatment and reported medicinal plants usage. Therefore, it was noted that in the riparian unit the relation between the nonadherence and medicinal plants usage is more predominant than in the urban healthcare unit, this could be associated with cultural factors, such as medicinal plant usage and also the difficult access to the medicine, making them turn to other alternatives to treat their pathologies. Financial support or fellowship: FAPESPA; UFOPA. CFSP109 MONITORING OF VANCOMYCIN LEVELS PROTOCOL: CONTRIBUTION OF THE CLINICAL PHARMACIST IN THE THERAPEUTIC OPTIMIZATION OF VANCOMYCIN MARIA APARECIDA DE SOUZA SILVA; ANA PAULA VIEIRA ARAUJO; JANBISON ALENCAR DOS SANTOS; NELMA LOURENÇO DE MATOS CRUZ; DANIELE HERNANDES COIMBRA MUNIZ; ANA CAROLINA MORANDI; ADIEL GÓES FIGUEREDO JUNIOR; BIANCA SILVA SVICERO. Universidade Federal de São Paulo, São Paulo - SP - Brazil. Introduction and objective: Monitoring of plasma levels of vancomycin is recommended because of the variability of the patient’s pharmacokinetic profile and consequently for the therapeutic optimization of Vancomycin. Having the pharmacist involved in the management of the levels of vancomycin is related to better clinical outcomes and economic benefit. The objective was to analyze the pharmaceutical recommendations and the rate of acceptance by the physician team involving the institutional monitoring of Vancomycin levels (MVL) protocol. Materials and methods: Retrospective descriptive study of Pharmaceutical Recommendations (PhRs) registered in the database of pharmacy services of general adult intensive care units and neurosurgery, from April 2015 to December 2016. The protocol recommends for Vancomycin: loading dose and maintenance; Frequency of Vancomycin serum, the first 30 minutes before administration of the 4th dose; Serum levels between 15 to 20 or 20 to 25 mcg/mL depending on the infectious focus; Dose adjusted or maintained according to the levels of Vancomycin. Based on the protocol, the PhRs were classified as: dose adjustment by weight; Whether or not Vancomycin serum is required; Dose increase or reduction, and whether or not the dose of Vancomycin is given. Results and conclusion: 522 PhRs were performed involving the MVL protocol, and 495 (95%) were accepted by the physician team,: the most frequent being vancomycin serum request with 285 PhRs and a 95% acceptance rate; Dose increase (88) with 95% acceptance; Dose reduction (79) with 97% acceptance; Non-dose administration (39) with 95% acceptance; No request of new vancomycin serum (22) with acceptance of 86%; Dose adjusted by weight (7) with 86% acceptance; Of administration of the dose (2) with 100% acceptance. The pharmacist inserted in the care team can contribute to the rational application of the MVL protocol, optimization of the therapeutic use of Vancomycin and greater patient safety.

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CFSP110 MEDICATIONS MOST COMMONLY USED IN SELF-MEDICATION BY CHRONIC HEMODIALITICAL RENAL PATIENTS LUCAS BRASILEIRO LEMOS1; NARA JACQUELINE SOUZA DOS SANTOS2; IONARA VIEIRA ROCHA DA MOTA2; GISELE DA SILVEIRA LEMOS2; ADRIANA ALVES NERY1. **Department of Health Ii, State University of Southwest Bahia (Susb), Jequié - BA - Brazil; 2*Department of Science and Technology, State University of Southwest Bahia (Susb), Jequié - BA - Brazil. 1

Introduction and objective: Self-medication guarantees the autonomy of the patient in the care process, but is considered as a risk-permeated practice, mainly in patients with chronic kidney disease (CKD), since the complexity of the disease requires a greater rigor in the use of medications. The objective of the study is to indicate the main drugs used by self-medication in patients with CKD in hemodialysis. Materials and methods: A cross-sectional study was performed with 170 patients from a hemodialysis clinic in the interior of Bahia, from March to May 2015, with the application of a form. The project was approved by the Research Ethics Committee of the State University of the Southwest of Bahia (SUSB) (Certificate nº. 957.553). Results and conclusion: The number of patients in whom drug use was identified by self-medication was 51 (30%). We identified 51 different medications used by these patients. The most used drugs were: Calcitriol (9.61%), Clonidine (6.73%), Nimesulide (5.77%), Sevelamer (5.77%), Dipyrone (5.77%), Anlodipine 88%), Iron Hydroxide (2.88%), Omeprazole (2.88%), Caffeine/Diclofenac/Carisoprodol/Paracetamol (2.88%) and Caffeine/Dipyrone/Orphenadrine (2.88%) and others (51.95%). Self-medication could be considered as a frequent practice among study patients. When considering the different risks patients are exposed to when using it, prescribers should give greater importance in the follow-up of patients with CKD, since in general, self-medication is neglected by these professionals. Financial support or fellowship: SUSB

MULTIPROFESSIONAL GUIDANCE FOR HOSPITAL DISCHARGE OF THE MARTERNITY

CFSP111

CECÍLIA MAYUMI ARAÚJO SATO1; GABRIELLA BRANCHINI ACCARINI1; KEMILLIN AMANDA RIBEIRO1; KRIZIA MESCHIATI1; ALINE CRISTINA PANÇA SUTTO1; ALESSANDRA COUTO BOAVA1; CRISTIANE ELOÍSA VENÂNCIO GUEDES2; ANDRÉIA CRISTINA ZAGO DA SILVA1. Universidade São Francisco, Bragança Paulista - SP - Brazil; 2Hospital Universitário São Francisco, Bragança Paulista - SP - Brazil. 1

Introduction and objective: In our experience in the HUSF, we found that there is a shortage of informative materials aiming at guidance for mothers. Concerned with the educational strategies for puerperal women, we elaborated a flyer that brings together information to be provided by the multiprofessional team. The main idea is to offer integral care, bringing together all the information in a single material, avoiding the occurrence of repetitive guidelines or even lack of them. The objective was to prepare informative material in flyer format. The material must have general guidance given to puerperal women in order to reduce them unknowingly taking incorrect measures due to the scarcity of information, thus seeking the improvement of health care and optimization of the work of orientation of hospital discharge. Materials and methods: For the elaboration of the technical information material, we first analyzed the routine of the sector. It was possible to verify where its deficiencies were. A critical analysis of the discharge process and materials involved in it was conducted. We sought to verify the interest and availability of the different professionals involved in the teaching-learning process aiming at the participation of all of them in the maternal education for discharge. Results and conclusion: Development of educational material, aiming at the hospital discharge of newborns and mothers of the maternity, undertaken with the participation of the multiprofessional team. Our study emphasizes the importance of the engagement of all professionals in the hospital discharge orientation process. This results in the minimization of the inquiries from mothers about domestic care for the newborn and herself.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

NOTICE OF ADVERSE EVENTS BY MULTIDISCIPLINARY HEALTH TEAM

CFSP112

GISELE DA SILVEIRA LEMOS1; IONARA VIEIRA ROCHA DA MOTA1; MARIO BORGES ROSA2; CRISTIANE A MENEZES DE PADUA3; EDSON PERINI3; LUCAS BRASILEIRO LEMOS1. Uesb, Jequie - BA - Brazil; 2Ismp Brasil, Belo Horizonte - MG - Brazil; 3Ufmg, Belo Horizonte - MG - Brazil.

Introduction and objective: Notification is a reactive tool for risk management and should be used in all health facilities to promote patient safety. This study evaluated the notifications made by a multidisciplinary health team in a regional reference public hospital. Materials and methods: Census survey using a translated and validated questionnaire (Hospital Survey on Patient Safety Culture - HSOPSC) with health professionals involved in patient care or administrative assistance related positions. Volume and type of professionals responsible for the notifications were analyzed. Data collection occurred from 12/2015 to 03/2016. Study approved by CEP/UFMG (CAAE: 49477015.6.0000.5149). Results and conclusion: The respondents were 331 (86%) professionals, 74.1% female, and mean age 38.7 ± 11.8 years. Staff composition: 44.0% nursing technicians/auxiliaries, 28.4% nurses, 17.1% doctors and 10.5% other. About half (51.4%) had graduate studies (lato sensu or strictu sensu). It was observed that 249 (78.3%) professionals did not report adverse events in the last 12 months, 44 (13.8%) reported from 1 to 2 and 19 (6.0%) reported 3 to 6 events. Our results demonstrate that in the hospital organization studied the vast majority of professionals did not make any notification in the last 12 months. There appears to be no well-established and routine adverse event reporting practice in the institution. Possible explanation is based on the prevalence of a punitive culture and the disinformation of the professionals regarding the importance of the notification, being clear the need for works that develop awareness and the training for notification. Financial support or fellowship: UESB CFSP113 PARASITOLOGICAL ANALYSIS OF WATER FROM URBAN WATERHOLES IN THE MUNICIPALITY OF MOGI GUAÇU, STATE OF SÃO PAULO, BRAZIL PAULO SÉRGIO MIRANDA; RENATA LOPES RODRIGUES; CAMILA STÉFANI ESTANCIAL FERNANDES; DANYELLE CRISTINE MARINI; NÁDIA REGINA BURIM ZUIM. Fimi, Mogi Guaçu - SP - Brazil. Introduction and objective: Water suitable for human consumption has a potable standard that does not pose a risk to human health. Such water is used for drinking, the preparation of meals, and personal hygiene. Biological agents, such as bacteria, protozoa, viruses and helminths, which mainly originate from human or animal fecal contamination, play a huge role in the contamination of water. Parasitic infections caused by protozoa and helminths affect approximately three billion people throughout the world and are mainly caused by the ingestion of contaminated water or food. The aim of the present study was to determine the presence of water-borne pathogenic parasites in waterholes. Materials and methods: Water samples were collected from three waterholes in urban areas of the municipality of Mogi Guaçu in the state of São Paulo, Brazil. Analysis involved either the spontaneous sedimentation technique described by Hoffman, Ponz and Janer or Lutz and Faust’s centrifuge flotation technique modified for water. Results and conclusion: No parasites pathogenic to humans were identified, but Endolimax nana and Entamoeba coli were found in two waterholes. Epidemiologically, these commensal protozoa are important markers of fecal-oral contamination in water or food. The water analyzed is used by the population and therefore subject to contamination due to the pollution generated in urban centers as well as the presence of animals in the vicinity of the waterholes. Awareness on the part of the population and commitment from municipal administrators could diminish the problem of the use and contamination of these waterholes, thereby preventing the occurrence of water-borne diseases in the municipality.

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CFSP114 PATIENT SAFETY CULTURE EVALUATION IN THE SURGICAL CENTER IN A REFERENCE MATERNITY SANDNA LARISSA FREITAS DOS SANTOS1; ATHILA WESLEY LIMA LACERDA1; NATALHA NAYANE DE OLIVEIRA PINHEIRO1; EMELINE MOURA LOPES1; FRANCISCO GILBERTO FERNANDES PEREIRA2; EUGENIE DESIRÉE RABELO NÉRI1. Universidade Federal do Ceará, Fortaleza - CE - Brazil; 2Universidade Federal do Piauí, Teresina - PI - Brazil.

Introduction and objective: Ordinance No. 529 of 2013 defines patient safety as a reduction of the risk of unnecessary injury related to health care, to a minimum admissible and an adverse event as an incident that causes harm to the patient. Objective: To verify the patient safety culture according to the time of work in the team of the Surgical Center of Maternity Assis Chateaubriand School-Fortaleza-CE, Brazil. Materials and methods: This is an exploratory, evaluative and cross-sectional study carried out from May to June 2016. The population was composed of 119 professionals with the application of the instrument Hospital Survey on Patient Safety Culture (HSOPSC) validated and translated into Portuguese. Data were analyzed statistically using the SPSS v.22.0 program and with measures of central tendency (mean), measure of dispersion (standard deviation) and standard error. Results and conclusion: It was observed that of the 119 participants 40 (20%) were physicians, with a higher prevalence of women, 97 (81%). According to the data, it was noted that 80.5% of professionals with less than one year of work stated that they did not perform any notification of adverse events. Among the professionals with a weekly workload of 20 to 39 hours, 100% stated that the hospital units work well together for the best care. Team discussion to prevent errors had a higher percentage (67%) in professionals with 1 to 5 years of work. The patient safety score was very good at 42% with a significant relation with the professionals working 100h a week. 74% of respondents said they did not make any notifications. In this context, knowing the safety culture is essential in defining strategies, to make assistance safer and provides a vision of the values of the care team. Financial support or fellowship: Federal University of Ceara CFSP115 PHARMACEUTICAL ACTION IN ORIENTATION FOR THE INDISCRIMINATE USE OF OMEPRAZOLE ACQUIRED IN THE COMMUNITY PHARMACY LUANA DE CARVALHO SANTANA. Estácio - Fib, Salvador - BA - Brazil. Introduction and objective: Pharmaceutical care includes defining the individual’s care needs and providing not only the necessary medicines but also services to ensure safe and effective therapy. It is based on an agreement between the patient and the pharmacist. In addition to effectively contributing to the rational use of medications, it directs the patient to an even more accurate and safe therapy, and plays an important role in the use of MIPs. As an example, we have the antiulcer drugs that are among the 10 best-selling pharmacological classes in Brazil, according to INTERFARMA and Omeprazole (antiulcer) is on the list of the best selling drugs in the world, according to JAMA (Journal of the American Medical Association). Although the drug is indicated for gastric ulcer and esophageal gastric reflux disease, health professionals warn of misuse in simple situations such as bloating. Materials and methods: A bibliographic review of papers published in the SCIELO, MEDLINE, and LILACS databases was carried out. Only published papers from 2007 to 2017 were included, and papers that did not correspond to this period and that did not report Omeprazol in their research were excluded. Descriptors: Pharmaceutical Care, Omeprazole, Pharmaceutical. Results and conclusion: Selected papers demonstrated the large number of important adverse effects associated with incorrect use of Omeprazole, ranging from anemia to osteoporosis and dementia in elderly patients, and even masking the habitat of an opportunistic bacterium such as Helicobacter pylori. They also show the lack of orientation of some prescribers who indicate the drug for a period beyond that recommended on the packaging, patients in polypharmacotherapy who use medication at incorrect time, decreasing or potentiating the effect of these medications, since Omeprazole alkalinizes PH of the stomach, while some medications need the acidic environment of the stomach to be absorbed. It is concluded that the indiscriminate use of Omeprazole is an issue that endangers public health due to the variety of pathologies that affect the misuse of this drug, but the pharmaceutical service can be used in an accurate way to minimize the problems of self-medication.

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CFSP116 PHARMACEUTICAL ACTIVITIES IN MULTIPROFESSIONAL RESIDENCY IN SUS PRIMARY HEALTH CARE IN THREE CITIES OF THE ABC - SP REGION: CASE REPORT AMANDA SALES CUSTODIO1; AMANDA CAVALCANTE VAZ DE MORAES2; ANDREA DE ANDRADE RUGGIERO1; ANA ELISA PRADO CORADI1; JULIANA LOPES CUSTODIO3. Fmabc, Santo André - SP - Brazil; 2Fmabc, São Bernardo do Campo - SP - Brazil; 3Fmabc, São Caetano do Sul - SP - Brazil.

Introduction and objective: The pharmacist is increasingly integrated in health teams, including SUS (sistema único de saúde). With emphasis on pharmacotherapy and rational drug use, this professional can conduct multiprofessional consultations, clinical case discussions, home visits, and health education directed to the user through projects such as Hiperdia, promotion groups, prevention and recovery of health; making it a privileged space for dialogue and production of knowledge, in which, through their technical knowledge, they share and covenants in an enriching way with the attached community, new approaches related to improving the quality of life of the population. Objectives: to address the perception and experiences of the pharmaceutical residents in the activities within the context of the Family Health Strategy Program in three cities of the ABC, pointing out the complexity and challenges regarding the insertion of the clinical pharmacist in the interdisciplinary team. Materials and methods: The study is based on experiences of activities developed by pharmacists of the Multiprofessional Residency Program in Health of the Elderly and Cancer Care, by the Faculty of Medicine of ABC, distributed in the cities of: Santo André, São Caetano do Sul and São Bernardo do Campo in the period from March 2017 to June 2017. Results and conclusion: The performance of the pharmacist in multiprofessional residency becomes relevant considering the new configurations and health relations established within the interdisciplinary work team. Each City has different approaches, depending on the resources of each location. However, some barriers must still be overcome so that some cities can include this professional in their Family Health Strategy Programs. Financial support or fellowship: ABC Medical School - FMABC CFSP117 PHARMACEUTICAL ANALYSIS OF MEDICAL PRESCRIPTIONS AND PATIENT PROFILE OF ADULT INTENSIVE CARE UNIT (ICU) IN A PRIVATE HOSPITAL IN SANTOS, SP, BRAZIL GUILHERME DA SILVA JONAS1; THAIS DAMIN LIMA2; JOSÉ MARCELINO ANTONIO1. Universidade Católica de Santos, Santos - SP - Brazil; 2Hospital das Clinícas da Fmusp, Santos - SP - Brazil.

Introduction and objective: The high amount of drug interaction occurring in ICU facilities is known, which enhances the necessity to continue studies on the subject. Objective: Understand patient profiles and drug interactions in adult ICU in a private hospital in Santos, SP. Materials and methods: Daily prescriptions related from 03/01/2016 to 04/30/2016 were analyzed. Studying possible interactions based on the literature and online platforms. Drug interactions, gravity, gender, age and reason for hospitalization were analyzed and plotted on an Excel table. Results and conclusion: Results and discussion: It was observed that 17% of prescribed medicines are included in the Potentially dangerous medications list. The study revealed high prevalence of drug interactions, especially moderate drug interactions. The frequency of interactions in critically ill patients may be high due to the high complexity of the situation, together with the large number of drugs they receive, on average 12 per prescription. Of all drug interactions found, 14.5% are classified as severe. The average patient age was 64.4 years old and the average hospitalization period was eight days. The main reason for hospitalization was neurologic diseases. This article investigated the most frequent major drug interactions and evaluates the ideal action to be performed. Conclusion: Knowledge of drug interactions is extremely important due to the high number of drugs in the therapy, which can become harmful at different levels during the hospitalization. This assists in medication standardization and protocol development. The importance of the pharmacist’s presence in the multidisciplinary team is noted, also acting as a health educator to the professionals involved in the drug therapy chain.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP118 PHARMACEUTICAL AND CONTROL OF HOSPITAL INFECTIONS: INDICATORS FOR USE OF ANTIMICROBIALS IN A HEALTH UNIT OF SÃO LUÍS – MA, BRAZIL FLAVIO DONALWAN SA MAXIMINO; ANA PAULA DE ARAUJO ABREU; POLLYANE SOARES. Faculdade Pitágoras, São Luís, Maranhão, Brazil- MA - Brazil. Introduction and objective: The pharmacist plays an important role in infection control by promoting the rational use of antimicrobials, identifying outbreaks and the emergence of resistant bacteria in the hospital environment. Rational use of antimicrobials improves treatment efficacy, reduces drug costs, minimizes adverse events, and reduces the potential for bacterial resistance. Analyze the indicators of use antimicrobials, the main causes of hospitalization and the actions of infection control at the public hospital. Materials and methods: A preliminary retrospective study of 30 days, in January 2017, of a descriptive observational character using the antimicrobial records, medical records and prescriptions as primary data. The following parameters were observed: clinical diagnosis and the main drugs dispensed. The patient data were not analyzed, following the ethical precepts. Results and conclusion: During this period, 12 diagnosed diseases were observed: pneumonia (06; 20%), urinary tract infection (05; 16.6%) and COPD (05; 16.6%). A total of 14 different antimicrobials were prescribed, including: ceftriaxone 1g (20; 41.8%), levofloxacin 5mg/ml (04; 8.10%) and cefepime 1g (04; 8.10%). Preliminary analysis of the data demonstrated the high use of antimicrobials in the health unit. The number of patients without a confirmed diagnosis, using prophylactic drugs, is highlighted. The pharmacist in hospital pharmacovigilance should contribute to infection control in a more active way, sensitizing the prescribers for a more adequate and rational use of antimicrobials. Future studies are required to quantify other parameters related to the use of antimicrobials in the health unit. CFSP119 PHARMACEUTICAL ASSISTANCE AND PUBLIC HEALTH THE CLINICAL PHARMACIST IN THE ACCOMPANIMENT OF PATIENTS WITH PORPHYRIA. ADNA VASCONCELOS FONTELES1; ANTONIA RAFAELLA FERREIRA GOMES MARTINS2; BRENA GEYSE MESQUITA ROCHA SOARES2; IANNE DE FATIMA SAMPAIO MARINHO2; CRISLIANE GOMES DE AMORIM LIMA3. Santa Casa de Misericórdia de Sobral/Inta, Sobral - CE - Brazil; 2Santa Casa de Misericórdia de Sobral/Inta, Sobral - CE Brazil; 3Santa Casa de Misericórdia de Sobral, Sobral - CE - Brazil. 1

Introduction and objective: The follow-up of patients with porphyria is based on the prevention of crisis. The education of patient, and identifying factors that can trigger it is very important in prevention. With this, the objective of this study is to report the follow-up of the clinical pharmacist in this population Materials and methods: This is an experience report, experienced through the hospital follow-up of patients with porphyria. The clinical pharmacist addresses and recognizes these patients during drug reconciliation. Results and conclusion: Initially, all the factors that may have triggered the exacerbation can be evaluated in several Brazilian, American and European lists, respectively, ABRAPO - Brazilian Association of Porphyria, APF-American Porphyria Foundation or European Porphyria Network. It must be verified whether all the drugs are safe in porphyria - because several medicines can trigger crises of the disease. These patients should avoid carbohydrate restriction or prolonged fasting diet, where it can be verified in the prescription and if necessary, there is an intervention with the multiprofessional team. Pharmacotherapeutic monitoring should monitor serum sodium levels, since standard therapy is based on intravenous infusion of glucose and may induce hyponatremia. The hematine or hemin therapy, a human tetracyclic ferric complex similar to human heme is the first choice for treatment, where in the discharge guidance the pharmacist should guide when to use and where to obtain this medicine, at it is of a high cost and difficult to access the drug, and during hospital discharge, to provide the list of safe and unsafe medicines for porphyria. Usually, the management of the porphyria crisis is done with the hospitalized patient. The prognosis is generally good, especially in those patients who are able to avoid triggering factors of the acute crisis that are often those due to the use of drug triggers. Financial support or fellowship: SANTA CASA DE MISERICÓRDIA DE SOBRAL; INTA

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP120 PHARMACEUTICAL CARE AND FOLLOW-UP CARRIED OUT IN A CORONARY INTENSIVE CARE UNIT IN A SPECIALIZED PUBLIC HOSPITAL MARIANA CAPPELLETTI GALANTE1; ALESSANDRA SANTOS MENEGON2; DANIELE CRISTINA GOMES2; DENIS DEQUIAN DE SOUZA SILVA2; SONIA LUCENA CIPRIANO3. Instituto do Coração Hcfmusp, São Paulo - SP - Brazil; 2Incor Hcfmusp, São Paulo - SP - Brazil; 3Incor Hcfusp, São Paulo SP - Brazil. 1

Introduction and objective: Pharmaceutical cares collaborates with all health professionals for patients to reach maximum effectiveness from the available pharmacotherapy. Objective: report pharmaceutical care results and follow-up patients hospitalized in coronary intensive care unit. Materials and methods: The prospective study was carried out from May 2016 to May 2017. For Pharmaceutical care and follow up, the following were performed: evaluation of medication prescriptions, medication reconciliation at hospital admission and discharge, pharmacovigilance reports and pharmaceutical orientation at hospital discharge. A form was used to register: medication reconciliation data, interactions and incompatibilities between medications and clinical exams. All procedures were recorded in the hospital’s electronic medical record system. The research was approved by Ethics Committee SDC 4501/17/001. Results and conclusion: During the study period, pharmaceutical care and follow-up were performed in 608 patients, of whom 65.8% were men. The study evaluated 2,057 drug prescriptions. Medication reconciliation was performed at admission in 566 patients (93.1%), and when patient had conditions to be discharged directly from the Unit, the reconciliation and pharmaceutical orientation at hospital discharge for 137 patients. During the pharmaceutical care and follow-up, 252 pharmacist interventions were performed, of which 174 (69%) were accepted and 78 (31%) were not accepted and 17 pharmacovigilance cases reported. Conclusion: The importance of pharmaceutical care and follow up integrated to the multidisciplinary team for the hospitalized patient, has been shown to avoid medication-related problems through the pharmaceutical interventions carried out, with positive impact on the hospitalized patient safety. CFSP121 PHARMACEUTICAL CARE FOR PATIENTS WITH DIABETES MELLITUS AND HYPERTENSION ON THE ISLAND OF MAIANDEUA/MARACANA-PARA. INGRID SILVA DE OLIVEIRA1; SANDRYNNE CARLA NEVES GUIMARAES1; ANDRÉA LÚCIA GUALBERTO DE OLIVEIRA SANTOS2; ELIAS JÚNIOR PEDREIRA DA SILVA2; DANILA TERESA VALERIANO ALVES1; CHRISTIAN NERI LAMEIRA1. Faculdade Integrada Brasil Amazônia - Fibra, Belém - PA - Brazil; 2Escola Superior da Amazônia - Esamaz, Belém - PA - Brazil.

Introduction and objective: The increase in the number of deaths due to chronic diseases such as Diabetes mellitus and arterial hypertension is a major concern, the treatments and control of which are linked to changes in the quality of life, in the diet and in the intake of medicines that compromise the quality of life. Pharmacotherapeutic monitoring is essential because it is through this that the pharmacist works to ensure the most indicated, effective and safe treatment. Objective: To provide Pharmaceutical Care to Hypertensive and Diabetic Patients living on Maiandeua island/Maracanã - PA. Materials and methods: The Dáder methodology was used for the care of patients. Intentional sampling is composed of 73 patients in the age group of 24 to 96 years, carriers of pathologies required in the study. Periodic visits were made over the period of one year, with the purpose of providing the monitoring of these patients, performing interventions in their lifestyle. Results and conclusion: Pharmaceutical interventions have become effective for prevention of adverse events, with prevalence of interventions aimed at patient education, reinforcing the role of the pharmacist, in patient care process and drug users. The pharmacotherapeutic follow-up of patients with CNCD demonstrated the importance of the correct use of the drug, as well as the role of the pharmacist in the prevention, promotion and recovery of health.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

PHARMACEUTICAL CARE FOR THOSE WITH HEARING DISABILITY

CFSP122

ABIA CRISTINA FELIPPE; CLAUDINEI ALVES SANTANA Instituito Seli, São Paulo - SP - Brazil. Introduction and objective: Pharmaceutical Care is fundamental in the follow-up of patients’ medical treatment. Communication between the pharmacist and the patient needs to be sympathetic to both parties. In the case of deaf patients, proper pharmaceutical care and communication become essential for pharmacotherapeutic treatment and follow-up. In the care of the hearing impaired, we consider it necessary to present the physiology of the hearing system and the causes that can cause dysfunction in this system, causing deafness. We also deal with the use of LIBRAS and its importance and relevance for the deaf community to have adequate care. Materials and methods: Methodology: 20 patients with hearing impairment were analyzed at the Seli institution, and 10 patients were followed by 1 interpreter of LIBRAS. Results and conclusion: Results and conclusion: in this work we highlight some tools that can subsidize Pharmaceutical Care as: use of oral communication for people who have difficulty hearing, orofacial reading use for people who understand the Portuguese language and can communicate through it, use of interpreter, use of LIBRAS, use of illustrations and figures, use of gestures and mime to communicate with the deaf patient, and facilitate the understanding of possible drug treatments. There was a positive return in the area of Pharmaceutical Care in hearing impaired patients: drug treatments were oriented and successfully completed. There is a need to create material to facilitate Pharmaceutical Care with LIBRAS signs for all healthcare professionals. CFSP123 PHARMACEUTICAL CARE FOR PATIENTS EVALUATED IN THE CLINICAL DENTISTRY OF THE SANTA CECÍLIA UNIVERSITY MONIKE SILVA DE FREITAS; LUCIANA LOPES GUIMARÃES; WALBER TOMA*. Universidade Santa Cecília, Santos - SP - Brazil. Introduction and objective: Pharmaceutical Care is highlighted, which is an interaction between the pharmacist and the patient, aiming to meet the needs related to medicines. Of the existing methodologies, the Dader Method is based on the patient’s pharmaceutical documentation in order to identify and resolve possible Negative Results Associated with the Use of Medications (RNM), in which pharmaceutical interventions are performed to solve them. Such data become important when integrated into Dentistry because the performance of safe dental surgical procedures also involves an evaluation of medications. For this reason, the objective of this study is to evaluate the RNM that promote risk to the patient. Materials and methods: After approval by the Ethics Committee of Unisanta (CAAE: 18441413.8.0000.5513), anamnesis and blood pressure measurement were performed in 25 patients attended at the clinical dentistry at the Santa Cecília University. This anamnesis was performed according to the patient’s medical history and afterwards, the Dader Method was applied in order to obtain Negative Results Associated with the Use of Medications (RNM). Results and conclusion: Of the 25 patients interviewed, 68% (n=17) used continuous medication, where 82.3% (n =14) used antihypertensive drugs, followed by 64.7% (n =11) users of antiplatelet agent and 41.2% (n = 07) using drugs to treat diabetes mellitus. Of the antihypertensive users, 21.4% (n=03) had blood pressure values outside the normal parameters, one patient being classified as having stage I hypertension and the other two patients as having stage II hypertension. During anamnesis, it can be observed that these patients did not use the medication according to medical prescription, characterizing these data as RNM by a quantitative ineffectiveness. These data demonstrate the importance of the pharmacist in the multidisciplinary team through the Dader Method, assisting in the prevention and promotion of health in the dental surgical scope.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

PHARMACEUTICAL CARE IN BRAZILIAN PATIENTS USING WARFARIN

CFSP124

LEILIANE RODRIGUES MARCATTO 1; LUCIANA SACILOTTO 1; CAROLINA TOSIN BUENO 1; MIRELLA ESMANHOTTO FACIN1; CELIA MARIA CASSARO STRUNZ1; FRANCISCO CARLOS DA COSTA DARRIEUX1; MAURICIO IBRAHIM SCANAVACCA1; JOSÉ EDUARDO KRIEGER1; ALEXANDRE DA COSTA PEREIRA1; PAULO CALEB JUNIOR LIMA SANTOS2. Incor, São Paulo - SP - Brazil; 2Unifesp, São Paulo - SP - Brazil.

Introduction and objective: Warfarin is the most widely prescribed oral anticoagulant agent worldwide. Warfarin has a narrow therapeutic range and, consequently, patients usually have difficultly achieving and maintaining the therapeutic target. The aim of this study was to implant the pharmaceutical care in Brazilian patients using warfarin. Materials and methods: We included 64 patients with atrial fibrillation and without stable dose of warfarin Results and conclusion: Of the 64 patients (mean age 66 ± 10), 33 (52.4%) were female and 49.2% were white. Mean dose was 28.7 mg/week on the last consultation. The median of INR value before the treatment was 1.6. After the 12 weeks with pharmaceutical care the median of INR value was 2.3. All participants finished the study. Treatment with pharmaceutical care is possible with a multidisciplinary team and can improve the therapy. Financial support or fellowship: FAPESP; CNPq; FQM. CFSP125 PHARMACEUTICAL CARE IN HYPERTENSIVE PATIENTS ATTENDED AT PHARMACIES IN PEREIRA BARRETO/ SP MARIA ANGÉLICA DE PAULA MENARDI*;; JOSELAINE DE OLIVEIRA; LUIS CEZAR FARIAS DE OLIVEIRA;; MARIANE MACHADO CURBETE. University Paulista – UNIP, Araçatuba - SP - Brazil. Introduction and objective: Pharmaceutical care consists of accompanying the patient and improving pharmacotherapeutic treatment, providing better quality of life for patients. Systemic arterial hypertension (SAH) is a multifactorial disease with a high blood pressure profile, usually asymptomatic, which causes patients to discontinue treatment and resume it only when the symptoms reappear, resulting in ineffective treatment. Objective: to evaluate pharmaceutical care and adherence of pharmacotherapeutic treatment of patients with SAH. Materials and methods: Cross-sectional, descriptive, observational and prospective study in three pharmacies (F1, F2, F3) at Pereira Barret, Sao Paulo state, using questionnaire containing dichotomous, multiple choice and semi-closed questions related to the demographic profile, lifestyle, medicines used and the pharmaceutical attention received by 45 patients with SAH. Results and conclusion: The majority of patients with SAH were women (F1 - 73.3%; F2 - 66.7%; F3 - 60.0%) aged 41-60 years (F3 - 66.7%, F2 - 46.7 %, F1 - 40.0%). The most commonly used pharmacological group were angiotensin II antagonists (F1, F2, F3 - 46.70%). The pharmacist was seen as a clerk, probably due to lack of pharmaceutical orientation (F2 - 86.70%, F3 - 33.30%, F1 - 20%), which corroborates with corroborated by the evaluation of the recognition of the pharmacist as a professional who cares for patients’ health (F1 - 80%, F3 - 66.70%, F2 - 13.30%). It was verified that some of the people do not have knowledge about the adequate antihypertensive treatment, disfavoring adherence to the same, as well as the correct and safe use of the medicines.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP126 PHARMACEUTICAL CARE IN THE ADHERENCE TO THE TREATMENT TO PATIENTS OF BIPOLAR MOOD DISORDER: CASE REPORT MARIA PAULA DE OLIVEIRA FERREIRA1; ANA ELISA PRADO CORADI2. Fmabc, Santo André - SP - Brazil; 2Fmabc, Santo Andre - SP - Brazil.

Introduction and objective: The pharmacist has much to contribute to the treatment of patients with mental disorders by improving communication with the patient and family in guiding them, promoting the rational use of the medication, in order to increase adherence to the treatment and the confidence of the family that a life is possible for such patients. Objectives: to show how the pharmacist can contribute to the treatment of these patients, in the context of a multidisciplinary team, in order to promote increased adherence to medication treatment and improvement of prognosis and quality of life and not only make it possible to access the drug. Materials and methods: A case report of a patient with bipolar mood disorder was carried out. A questionnaire was applied to the patient, regarding his clinical history and current clinical signs, investigating whether the patient was given adequate medical orientation in his treatment, the difficulties or facilities during the implementation of the drug treatment. Results and conclusion: Among the relevant pharmaceutical interventions in this case, the following stand out: Patient/caregiver advice on the specific treatment, on general treatment, on non-pharmacological measures, on drug storage; Suggestion of change in initial therapy; Referral to a structured education program since the beginning of the diagnosis; Provision of material on medication dosage schedules, printed educational materials and tablet organizers, or devices to aid adherence to treatment. Thus, the pharmacist can contribute to better adherence and promote improvement of the quality of life of patients and their families. CFSP127 PHARMACEUTICAL CARE IN THE TREATMENT OF CHEMICAL DEPENDENTS AT THE ALCOHOL AND DRUGS PSYCHOSOCIAL CARE CENTER – CAPSad TAYNA TORRES TORTELOTE; JAÍSE SILVA FERREIRA. Faculdade de Medicina de Campos, Campos dos Goytacazes - RJ - Brazil. Introduction and objective: Pharmaceutical care aims to ensure safe and effective pharmacotherapy for the patient who is seeking adherence to the treatment. The pharmacist who exercises this practice can guide patients with different pathologies, such as chemical dependency. Chemical dependency is characterized as a chronic disease which can accompany the individual throughout life and is amenable to treatment. Thus, the objective of the study is to analyze whether the pharmacist’s participation, with the pharmaceutical care service, increases patients’ adherence to the treatment of chemical dependency in an Alcohol and Drug Psychosocial Care Center (CAPSad). Materials and methods: The present study was a cross-sectional one of 20 male and female chemically dependent patients taken care at the Alcohol and Drugs Psychosocial Care Center – CAPSad, in the municipality of Campos dos Goytacazes - RJ, during the months of March and April, 2017. The procedure of data collection was performed through interview with each of the patients. Results and conclusion: Most of the interviewees were between 40 to 49 years old and 60 to 69 years old, with predominance of males. Regarding schooling, 85% of the patients have not finished complete elementary education. It was found that 40% of the patients were addicted to licit and illicit drugs together, such as marijuana, cocaine, crack and alcohol, and there was a relapse rate of 70 out of the 100% of the patients interviewed. It was observed that the pharmacist had a great participation in relation to patients’ medication use orientation. The data show that adherence to treatment does not depend only on the pharmacist, but on other health professionals, along with society as a whole, but mainly on the patient.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP128 PHARMACEUTICAL CARE SERVICE PROFILE IN A COMMUNITY PHARMACY IN THE CITY OF FORTALEZACEARÁ. ALEXANDRE CARDOSO LIRA1; TUÍRA FARIAS BRITO2; ANDRÉA BESSA TEIXEIRA3; TICIANA PRACIANO PEREIRA3. Graduado do Curso de Farmácia da Faculdade de Ensino e Cultura do Ceará - Faece, Fortaleza - CE - Brazil; 2Farmacêutica do Empreendimentos Pague Menos S/A, Fortaleza - CE - Brazil; 3Docente do Curso de Farmácia da Faculdade de Ensino e Cultura do Ceará - Faece, Fortaleza - CE - Brazil. 1

Introduction and objective: The pharmacist profession has been going through changes in Brazil and rising to prominence by the people. In this context, the community pharmacy, defined as a unity provider of pharmaceutical care and health following the Law nº 13.021/14, has becoming an important field for the practice of pharmaceutical care service (PC). The present study had the objective of describing pharmaceutical services performed in a community pharmacy in the city of Fortaleza-CE and the profile of customers served. Materials and methods: A cross-sectional and descriptive study was carried out, with a quantitative approach utilizing data collected from customer registrations provided by the company’s PC. The evaluated data was gathered from registered customers who were supported by the pharmacy personnel for not less than three months (n=30). Results and conclusion: The results observed that 56.7% were male and over 60 years old (60%). In addition, 50% of the customers presented class 1 obesity (B.M.I = 30.2), and 35.7%, hypertension related to diabetes, which implies significant risk of cardiovascular diseases. Concerning the services provided, 43% corresponded to pharmaceutical orientation (use of medicines, self-medication risk, healthy diet, and physical activities relevance), 32% blood pressure measurement, and 25% glucose test. It is also important to emphasize that the customers who possess hypertension related to diabetes had weekly support by the pharmaceutical customer service, and the others, only monthly. As a result, the study carried out observed that the arterial parameters of 100% of the research sample lead to controlled levels after the beginning of the pharmaceutical follow-up. Therefore, it is essential to focus attention on the great applicability of the clinical practice by pharmaceutical professionals within the scope of community pharmacies with the intent of providing health care to the people. CFSP129 PHARMACEUTICAL CARE: EVALUATION OF DRUG INTERACTIONS AMONG ELDERLY PATIENTS THROUGH AN ANALYSIS OF PRESCRIPTIONS BRUNA JACINTO; NÁDIA REGINA BURIM ZUIM; CAMILA STÉFANI ESTANCIAL FERNANDES; RENATA LOPES RODRIGUES; DANYELLE CRISTINE MARINI. Fimi, Mogi Guaçu - SP - Brazil. Introduction and objective: Elderly individuals constitute the age group that consumes the most medications due to the progressive loss of functionality of the organs. However, polypharmacy poses a risk of adverse drug interactions. The indiscriminate use of drugs may be the result of oversights at the time of prescribing such drugs. The aim of the present study was to evaluate the number of medications used by individuals aged 65 years or older through an analysis of prescriptions, focusing on potential drug interactions and the risks posed to patients. Materials and methods: This study was conducted at a pharmacy in the municipality of Mogi Guaçu in the state of São Paulo, Brazil, following approval from the local ethics committee (certificate number: 43467715.0.0000.5679). Data from 100 medical prescriptions were analyzed. Complementary information was collected through the administration of a questionnaire. Results and conclusion: Arterial hypertension was the disease with the highest incidence; 81 individuals took medications that act on the cardiovascular system. Polypharmacy (five or more medications) was found in 56% of the prescriptions. With regard to self-medication, 96% of the interviewees reported making use of a drug at times of pain, the most frequent of which was dipyrone. Potential drug interactions were detected in 82 prescriptions and 10 medications were identified as potentially inappropriate for elderly individuals according to the Beers-Fick criteria. The findings underscore the importance of the pharmacist in detecting flaws in geriatric prescriptions to avoid the inappropriate dispensing of drugs and adverse effects on the health of patients.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP130 PHARMACEUTICAL INTERVENTION IN DRUG CONCILIATION IN POLYMEDICATING PATIENTS AT A PRIVATE HOSPITAL IN SANTOS LEVI MATHEUS RODRIGUES DOS SANTOS1; VIVIANE RIBEIRO ZERBINATTI2; CARLA LANZA BELMONTE3. Unisantos, Santos - SP - Brazil; 2Hospital São Lucas, Santos - SP - Brazil; 3Universidade Católica de Santos - Unisantos, Santos - SP - Brazil. 1

Introduction and objective: The elderly represent a large part of the population of Santos, SP. Physiological changes due to age, comorbidities and the need for polipharmacotherapy make them highly susceptible to the occurrence of drug-related adverse events. Pharmaceutical intervention in drug conciliation demonstrates quality in the services provided at the hospital. The objective of this study was to carry out data collection through drug collection forms which patients use continually at home, collected at hospital admission, in order to discuss the pharmaceutical intervention in the medication conciliation for safe medication usage. Materials and methods: This is a retrospective cross-sectional study. All medication forms were collected at the hospital admission from hospitalized patients from April 2013 to September 2016, the drugs were collected by the hospital’s nursing team and analyzed and validated by the pharmacy team. The data were tabulated and analyzed by Excel programs for Windows through tables and charts analyzing averages and frequencies. Results and conclusion: After data collection, the cases of major pharmaceutical intervention were in elderly patients> 65 years of age, being poly-medicated (4 or more drugs). Among the results, the most relevant, representing 40% of the prescriptions, the drug was suspended and replaced by the representative drug belonging to the hospital standardization list, 30% represents patients who had their drugs suspended as its usage it is not justifiable in hospital or that the drugs brought by the patient presented poor conservation conditions. 20% of patients had oral glycemic controlling medication suspended for insulin therapy following the institutional protocol and finally in 10% of cases, the medications were suspended due to emergency surgical procedures. The active presence of the clinical pharmacist is related to the quality and safety of the patient, which through actions is observed improvement of health services and achievement of satisfactory therapeutic results.

PHARMACEUTICAL INTERVENTIONS CARRIED OUT IN AN INTENSIVE THERAPY UNIT

CFSP131

ALEX JUNIOR FERREIRA. Hospital São Lucas, Ribeirão Preto - SP - Brazil. Introduction and objective: The performance of the clinical pharmacist in an Intensive Care Unit (ICU) is realized through the pharmaceutical assessment of the medical prescriptions, which allows early identification of possible problems related to medications and prescription. The constitution of database with types of interventions realized during the evaluations is essential for improving pharmaceutical assistance and is an essential tool to ensuring improvements in drug therapy and multiprofessional team processes. The present study aimed to identify the main types of accepted and justified interventions realized in the period from March/2016 to March/2017 through analyses of medical prescriptions in the Adult ICU of a private hospital in the interior of the State of São Paulo. Materials and methods: This is a quantitative and descriptive study of the actuations of clinical pharmacists to patients hospitalized in the referred unit. The problems encountered related to medical prescriptions were discussed daily with the intensive care doctors through pharmaceutical suggestions and evolved in medical records, where it was up to the doctor to accept them or not. The data was inputted into Microsoft Excel for compilation, evaluation and development of indicators. Results and conclusion: In the period 3,661 prescriptions were analyzed and 1,244 suggestions made. Of the types of suggestions 34.2% (425) were justified and 65.8% (819) accepted by the doctors. Of the suggestions realized 37% were related to drug interactions, 36% the route of administrations (pathway correction (130) and pathway transition (312)); 17% drug-related problems (duplicate (82), drugs change (40), dosage (34) and drug addiction (26), incompatibility (19) and time of administration (6)); 5% prophylaxis of venous thromboembolism; 3%(38) antimicrobials and 2%(31) related to other types and interventions. In the suggestions realized, the most accepted were pathway transitions (33%), drug interactions (10%), prophylaxis of venous thromboembolism and drug duplication (5%). It was concluded by the present study that the performance of the clinical pharmacist with the multiprofessional team contributes to the identification of problems related to drug therapy, ensuring greater safety in decision-making related to drug safety and therapeutic efficacy, and contributing to the transition of drug pathways to help in discharge, aiming to improve quality of care of patients of this unit. Financial support or fellowship: Hospital São Lucas

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP132 PHARMACEUTICAL INTERVENTIONS CARRIED OUT WITH CORONARIOPATHIC PATIENTS IN A SPECIALIZED PUBLIC HOSPITAL MARIANA CAPPELLETTI GALANTE1; JÚLIA SUMIE NAKAIMA FUGITA2; ALESSANDRA SANTOS MENEGON2; ERIKA CORDEIRO DE LIMA2; GILCIMAR SALVIANO2; RENATA PARDUCCI DE OLIVEIRA2; SONIA LUCENA CIPRIANO2. Instituto do Coração Hcfmusp, São Paulo - SP - Brazil; 2Incor Hcfmusp, São Paulo - SP - Brazil.

Introduction and objective: Introduction: Pharmaceutical intervention according to the “Consenso Brasileiro de Atenção Farmacêutica” (2002) is a planned, documented and performed action with patients and health professionals, which aims to solve or prevent problems that interfere or can interfere in pharmacotherapy, being an integral part of the pharmacotherapeutic followup process. Objective: To report acceptance and categorization of pharmaceutical interventions performed in patients who were hospitalized for myocardial revascularization. Materials and methods: Prospective study carried out in patients hospitalized for myocardial revascularization surgery from June 2016 to June 2017. Pharmaceutical interventions were identified in: pharmacotherapeutic care and follow-up, evaluation of medication prescriptions, medication reconciliation at hospital admission and discharge, pharmacovigilance reports and pharmaceutical orientation at hospital discharge. A form was used for collecting data. All procedures and pharmaceutical interventions were recorded in the hospital’s electronic medical record system. Research approved by the Ethics Committee for Analysis of Research Projects under the number of opinion 1.838.298. Results and conclusion: Results: During the study period, 687 patients were admitted and 3236 drug prescriptions were evaluated. There were 257 pharmaceutical interventions, in which 205 (79.76%) were accepted and 52 (20.24%) were not accepted. Interventions were classified into 7 (seven) categories with the following results: medication conciliation 100 (39%), therapy adequacy 55 (21%), team/patient orientation 36 (14%), posology 32 (13%), pharmacovigilance 12 (5%) and interaction and drug incompatibility 16 (6%) and 6 (six) (2%) interventions of other types. Conclusion: Pharmaceutical team actions were of great importance for the promotion of patient safety in the use of prescribed pharmacotherapy, preventing drug related problems against expected clinical outcomes. CFSP133 PHARMACEUTICAL INTERVENTIONS IN THE CARDIOLOGY SECTOR OF A UNIVERSITY HOSPITAL LORENA DE SOUSA MIRANDA; MARIANA DUARTE DAVID LADEIA; RAFAEL MOTA PINHEIRO. University Hospital of Brasilia/Ebserh/University Of Brasilia. Sgan 605 L2 North, Brasilia - DF - Brazil. Introduction and objective: Pharmaceutical intervention is a practice that assists in the identification and resolution of drugrelated problems. Objectives: To analyze and quantify the pharmaceutical interventions performed during the revision of medical prescriptions in the cardiology sector of the University Hospital of Brasilia (HUB). Materials and methods: A retrospective observational study of the routine of the sector was carried out from July 2016 to June 2017, where the interventions performed were analyzed and quantified by two resident pharmacists of the HUB Cardiopulmonary Care program. The study was based on the pharmacotherapeutic follow-up of hospitalized patients, through review of medical prescriptions, analysis of laboratory tests and interviews. Interventions were conducted with medical staff during a daily visit to the patients and subsequently the data were recorded in a pharmacotherapeutic follow-up sheet adapted from a document from the French Society of Clinical Pharmacy. Results and conclusion: During the study, a total of 335 medical prescriptions were analyzed and 70 pharmaceutical interventions were performed. In the review of pharmacotherapy, 43% of the prescriptions were in non-compliance (n=30), 17% had an untreated indication (n=12), 10% possible interactions (n=7), in another 10% some subdose (n=7) and 4% superdose (n=3), 10% had drugs with inappropriate route or administration (n=7), 3% with some drug in need of monitoring (n=2) and in 2 prescriptions were observed an incident with damage (adverse events) (3%). In this context, the pharmaceutical interventions were: 48% optimize the administration (n=34); 19% add (new prescription) (n=13), in 14% dose adjustment (n=10); 10% drug suspension (n=7), 6% therapeutic monitoring (n=4), and 3% suggested substitution of a drug (n=2). Of the 70 interventions proposed, 86% were accepted by the prescribers. The study demonstrated the importance of the pharmacist through integration with the multidisciplinary team contributing to patient care. It was possible to observe that the performance of the pharmacist can generate great impact related to the improvement of safety and prevention of adverse events in the hospital context. Financial support or fellowship: Ministry of Education; Multiprofessional Residence in Health.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP134 PHARMACEUTICAL ORIENTATION ON HOSPITAL DISCHARGE OF PATIENTS WITH BREAST CANCER, POSTOPERATIVE: THE IMPORTANCE OF THE PHARMACIST. BRUNA SARTORATO RIBEIRO. Hospital Sirio Libanes, São Paulo - SP - Brasil. Introduction and objective: Breast cancer is the neoplasm that most affects women around the world and the first choice in treatment is usually surgery. The risk of thromboembolism is increased in these patients and they normally use thromboembolism prophylaxis with anticoagulants. The aim of this study was to analyze the discharge orientations of the patients in the post-operative period from the philanthropic program for patients with breast cancer in the philanthropic hospital in São Paulo. Materials and methods: A descriptive study based on analysis of the internal database using Excel® during the period from June 2016 to July 2017. The following data were found: total number of orientations, length of hospital stay, the professional who carried out the orientation and the need for specific orientations for anticoagulants use in addition to symptomatic medications at discharge. Results and conclusion: During the 14-month period, 327 patients were oriented, with an average of 23 patients/month. Of these, 297 (90.8%) were oriented by the clinical pharmacist and 30 (9.2%) by the nursing team. From the total number of patients, 224 (68.5%) were discharged with anticoagulant prophylaxis for venous thromboembolism: 219 (98%) with enoxaparin and 5 (2%) with rivaroxaban. The discharge orientation by the pharmacist is fundamental for the patient to know the risks involved in their post-operative period. CFSP135 PHARMACEUTICAL PROFESSIONAL ACTIVITIES IN A CENTER FOR PSYCHOSOCIAL CARE FOR CHILDREN AND ADOLESCENTS: SPECIALIST ORIENTATION, REFERENCE, THERAPEUTIC GROUPS AND WORK OF AMBIENCE - THE WORK BEYOND PHARMACY LUCAS DE SOUZA DETONI. Farmacêutico No Serviço de Saúde Dr. Cândido Ferreira/Capsij Espaço Criativo, Campinas - SP - Brazil. Introduction and objective: Attention to the individual with severe mental disorders in childhood and youth should be based on the construction of therapeutic projects, promoting the increase of protection factors and the reduction of risk factors. The promotion of health in a service Center for Psychosocial Care for Children and Adolescent is carried out by a multi-professional and multidisciplinary team, in which the pharmacist is inserted and acting on fronts beyond the action of dispensing medicines, an integral part of pharmaceutical care. The aim of this study is to present the reorientation of pharmaceutical assistance to the care of children’s mental health policies from the psychiatric reform, considering the effective participation of the pharmacist, provoking new forms of health work, through the narrowing of pharmaceutical care in the multi-professional team in mental health. Materials and methods: Through a qualitative method, we used the fact sheets prepared by a multi-professional team, guideline manuals on specialist orientation and referral services in the health services, guidance on groups developed with a multidisciplinary team. Results and conclusion: The pharmacist, as a health professional, is an important piece in the conception of a multi-professional team in a Center for Psychosocial Care for Children and Adolescent. The pharmacist can perform activities beyond pharmaceutical care, expanding the use of pharmaceutical care in patient care. The availability of the pharmacist in the service in the actions of specialist orientation, referrals, groups and work of ambience, brings a new perspective of care to the individual who is in mental suffering, being able thus to participate actively in the construction of the therapeutic project of the patients and in their psychosocial rehabilitation.

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CFSP136 PHARMACEUTICAL RESIDENT PERFORMANCE IN A MULTIPROFESSIONAL TEAM WITH EMPHASIS IN HOME HEALTH CARE LARISSA ABRAHÃO DA CRUZ. Hucff/Ufrj, Rio de Janeiro - RJ - Brazil. Introduction and objective: Home Health Care (AD in Portuguese) was established at SUS in 2011, being defined as a modality of substitutive health care or complementary to those already existing, characterized by a set of actions to promote health, prevention and treatment of diseases and rehabilitation provided at home, with guaranteed continuity of care and integrated into health care networks. In the perspective of AD, the pharmacist´s performance is mainly focused on pharmaceutical care according to the Pan American Health Organization (PAHO, 1995), it can be defined as the professional practice that aims to benefit the patient through the rational use of medicines, providing quality of life through the actions of the pharmaceutical professional, as well as their conduct, commitment, responsibility, ethical values and knowledge. Objective: Present the activities developed by the clinical pharmacy at the Home and Interdisciplinary Health Care Program (PADI in Portuguese) of HUCFF_UFRJ along with the multiprofessional team of the Program. Materials and methods: Descriptive, exploratory study under development about clinical pharmacist performance in a multiprofessional team at PADI. The volume of distribution (VD) by the clinical pharmacist aims to identify the Drug Related Problems (PRM in Portuguese) for pertinent guidelines that favor the rational use of medicines, for this purpose the present paper work counts on the elaboration of interviews as an instrument to identify PRM. Results and conclusion: Pharmaceutical interventions were carried out in the pharmacotherapeutic follow-up of the clients regarding the best pharmacological therapy management of the patient by the caregiver and correct medication storage. Conclusion: The clinical pharmacist´s performance to those clients in need of home health care, integrated with multidisciplinary actions, optimizes pharmacotherapy, reflecting on the safety and effectiveness of the assistance provided to this clientele. Financial support or fellowship: Fellowship: Ministério da Educação, Brazil CFSP137 PHARMACIST INTERVENTIONS ON DISCHARGE IN PEDIATRIC PATIENTS AT A LARGE PRIVATE HOSPITAL LUCIANA MELO DE ARAUJO1; ANA BEATRIZ DO VALLE FERNANDES1; ESTER EMERICK ELLER1; JULIANA RIBEIRO DE ASSIS1; RENATA MENDES ALVES BRANDÃO1; ROBERTA BORGES DOS SANTOS1; JULIANA FERNANDES DA SILVA2; JAQUELINE PILON DE MENESES1. Crf, São Paulo - SP - Brazil; 2Hospital Santa Catarina, São Paulo - SP - Brazil.

Introduction and objective: Pharmacist medication counseling and education on discharge is the process conducted according to the medical delivered prescriptions. Objective: To demonstrate the importance of pharmacist medication counseling and education based on institutional taxonomy in pediatrics on hospital discharge due to this complex population. Materials and methods: A retrospective cohort study was conducted with pediatric patients in medical/surgical units between January, 01, 2016 and December, 31, 2016. The data were performed through a direct database containing pharmacist interventions based on an adapted taxonomy version of the National Coordinating Council for Medication Error Reporting and Prevention (NCC MERP). The number of prescriptions discharged with some pharmacist intervention was evaluated. Results and conclusion: 438 pharmacist medication counseling and education on discharge were performed. Of these, 33 (8.0%) had some pharmacist intervention. These pharmacist interventions were classified as following: improper dose (27%), noncompliance in the medication process (27%), omission (24%), wrong drug (9%), wrong pharmaceutical form (6%) and other (6%). It is important to have a prescription analyzed by the clinical pharmacist on hospital discharge improving patient safety.

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CFSP138 PHARMACIST PARTICIPATION IN MULTIDISCIPLINARY ROUNDS AT INTENSIVE CARE UNITS: DESCRIPTION OF PHARMACIST RECOMMENDATIONS JANBISON ALENCAR DOS SANTOS; BIANCA SILVA SVICERO; MARIA APARECIDA DE SOUZA SILVA; ANA PAULA VIEIRA ARAUJO; DANIELE HERNANDES COIMBRA MUNIZ; ADIEL GÓES FIGUEREDO JUNIOR; ANA CAROLINA MORANDI; NELMA LOURENÇO DE MATOS CRUZ. Universidade Federal de São Paulo, São Paulo - SP - Brazil. Introduction and objective: The integration of clinical pharmacists in Intensive Care Units (ICU) contributes to the rational usage of medicine, assuring patient safety and lowering costs. Despite the importance of pharmacists’ clinical activities in these units, some countries are still developing them. Objective describe the Pharmaceutical Recommendations (PhRs) made during pharmacists’ clinical activities at the ICU. Materials and methods: A retrospective descriptive study of PhRs from the pharmacy at the General Adult ICU database, with seventeen beds, from January to December 2016. The PhRs were made by the pharmacists during multidisciplinary visits – when required – and then registered on a database. The PhRs were classified as: incomplete medical prescription; interval period for administrating medication; dilution or infusion period; method of administration; standardized medication; prophylaxis; unnecessary medication; medicinal indication; dosage adjustment; drug interaction; serum level monitoring, amongst others. The data were analyzed according to descriptive statistics. Results and conclusion: 1,900 medical prescriptions of 459 patients were analyzed, and 3,199 pharmaceutical recommendations were registered with a 90% acceptance rate. Of the 3,199 recommendations, the majority were classified into the following categories: medicinal indication 646, dosage adjustment 480, incomplete medical prescription 346, serum level monitoring 337, method of administration 334; dilution or infusion period 216, and prophylaxis 146. Vancomycin was the main medication incorporated into the PhRs, 498. The medicinal group with the largest number of PhRs was the anti-infective systemic use group, with 1,256. Clinical pharmacists contribute to the rational usage of medicine and to maintaining quality of multidisciplinary round given to the patient. CFSP139 PHARMACIST’S OFFICE: PHARMACEUTICAL SERVICES FOR POLYMEDICATED PATIENTS ASSISTED IN SCHOOL PHARMACY LAURA JÚLIA ESQUITINI LOPES1; CECILIA TORQUETI DE BARROS2; CRISTIANE DE CÁSSIA BERGAMASCHI2; TÂNIA REGINA FERREIRA2. Uniso, Sorocaba - Brazil; 2Uniso, Sorocaba - SP - Brazil.

Introduction and objective: Pharmaceutical care services can contribute to the reduction of admission or hospitalization time, to the care of people with chronic diseases, to the practice of health education and to a more cost-effective therapeutic intervention. This study aimed to implement a proposal for pharmacotherapy follow-up to polymedicated patients assisted in school pharmacy. Materials and methods: The pharmacotherapy follow-up was developed in Community Pharmacy Vital Brazil, located in the Pontifical Catholic University, in Sorocaba, State of São Paulo, Brazil. The service consisted of monthly interviews with patients with specific orientations about the medicines used in the treatment of hypertension and diabetes mellitus and evaluation of parameters that control these diseases, including blood pressure measurement, capillary blood glucose testing and identification of the body mass index. Results and conclusion: Since 2013, when the project was implemented in the Community Pharmacy Vital Brazil, 78 patients have been registered. Of these patients, 26 (33.3%) showed significant improvements in laboratory tests due mainly to better adherence to treatment and physical activity in daily routine, in addition to improvement in eating habits. Of this total, only 3 (3.85%) patients did not show improvement, presenting decompensated diabetes and problems with adherence to prescribed treatment. This project benefited the service users of the pharmacy, improving their health and also contributed to the training of pharmacy students for clinical practice of pharmaceutical care. Financial support or fellowship: Institutional Scholarship Program.

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CFSP140 PHARMACISTS’ CONTRIBUTIONS TO ADMINISTRATING SYSTEMIC USE OF ANTI-INFECTIOUS MEDICATION AT AN INTENSIVE CARE UNIT (ICU) OF A PUBLIC HOSPITAL JANBISON ALENCAR DOS SANTOS; NELMA LOURENÇO DE MATOS CRUZ; MARIA APARECIDA DE SOUZA SILVA; ANA PAULA VIEIRA ARAUJO; DANIELE HERNANDES COIMBRA MUNIZ; ANA CAROLINA MORANDI; ADIEL GÓES FIGUEREDO JUNIOR; BIANCA SILVA SVICERO. Universidade Federal de São Paulo, São Paulo - SP - Brazil. Introduction and objective: Pharmacist Recommendations (PhRs) regarding the administration of antimicrobials contribute to optimizing treatment, minimizing adverse effects, reducing antimicrobial resistance and ensuring the use of cost-effective treatments. The objective was to analyze the acceptance rate of PhRs implemented at an ICU that involved systemic usage of anti-infective medication. Materials and methods: A retrospective descriptive study of PhRs of the pharmacy at the General Adult and neurosurgery ICU database was used, over the period between April 2015 and December 2016. The database was analyzed and PhRs related to antiinfective medication were reclassified as: addition of new anti-infective medication, de-escalation; information about anti-infective medication; monitoring of serum levels; dose optimization; posology optimization; withdrawal of the anti-infective medication; substitution of the use of anti-infective medication by patients and sequence therapy. Results and conclusion: 2,137 pharmaceutical recommendations were identified. After being reclassified, the most frequent were optimization of posology 1,105, optimization of dose 508 and monitoring of serum levels 360. The main types of anti-infective medication were Vancomycin, Meropenem, Polymyxin B and Fluconazole; corresponding to 1,338 of the PhRs. The recommendations related to Polymyxin had the lowest acceptance rate among the anti-infective medication, corresponding to 89%, while Vancomycin had the highest acceptance rate corresponding to 93%. The global acceptance of the recommendations was 91%, with monitoring of serum levels being most accepted, at 94%. The de-escalation was least accepted, at 33%. High overall acceptance of the Pharmacists’ Recommendations shows the importance of pharmacists’ contributions in optimizing antimicrobial therapy in the intensive care environment. CFSP141 PHARMACOEPIDEMIOLOGICAL CHARACTERIZATION OF PATIENTS WITH HEPATOCELLULAR CARCINOMA IN USE OF SORAFENIBE: AN ANALYSIS OF QUALITY OF LIFE, PROFILE OF ADHERENCE TO TREATMENT AND ADVERSE REACTIONS RAFAEL NOGUEIRA DE SOUZA; NATALIA DA COSTA DUARTE; GRAZIELE BALDAN FERRARI; PATRICIA MORIEL. Universidade Estadual de Campinas - Unicamp, Campinas - SP - Brazil. Introduction and objective: Hepatocellular carcinoma (HCC) is the sixth most common cancer in the world and considered the third largest cause of cancer mortality. When reaching more advanced stages of the disease, patients are no longer eligible for surgery, and administration of the oral anti-neoplastic sorafenib is the only protocol adopted as first-line treatment. Sorafenib may cause serious adverse effects that may compromise patient’s quality of life and adherence to therapy. Objectives: describe demographic clinical data of patients undergoing treatment with sorafenib. The parameters analyzed will be quality of life, adherence to treatment and incidence of Hand-Foot Syndrome. Materials and methods: This is a retrospective and prospective observational study approved by the Ethics Committee (CAAE: 49975915.4.0000.5404). Patients were followed up for 3 cycles of chemotherapy. Knowledge about treatment was analyzed by the MedTake test, liver function by Child-Pugh score and adherence by Morisky-Green. Quality of life was measured before the first and after the third cycle through the EORTC-QLC-30 (general) and EORTC-QLC-HCC-18 (HCC specific) questionnaires. The incidence of Hand-Foot Syndrome was assessed according to the patients’ reports. Results and conclusion: Clinical data of eighteen patients undergoing treatment with sorafenib showed a predominance of males in HCC patients (17 men, 1 woman). 70.6% had high adherence to treatment and 56% reported Hand-Foot Syndrome grade 1, 2 or 3. It is hoped to increase the number of patients in the study to make the data more statistically relevant. Financial support or fellowship: CNPq

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CFSP142 PHARMACOKINETIC-PHARMACODYNAMIC APPROACH OF UNBOUND PLASMA PROPOFOL DURING CORONARY SURGERY WITH AND WITHOUT CARDIOPULMONARY BYPASS CARLOS ROBERTO DA SILVA FILHO1; RICARDO ANTONIO G. BARBOSA2; CARLINDO VIEIRA DA SILVA JR1; MARIA JOSÉ C. CARMONA2; DAVID DE SOUZA GOMEZ3; SILVIA REGINA CAVANI JORGE SANTOS1. Fcf USP- Clinical Pharmacokinetics Center, School of Pharmaceutical Sciences, São Paulo - SP - Brazil; 2Incor HcfmuspDivision of Anesthesiology, São Paulo - SP - Brazil; 3Hcfmusp-Division of Plastic Surgery and Burns, São Paulo - SP - Brazil. 1

Introduction and objective: The objective of this study was to apply a pharmacokinetic-pharmacodynamic (PK/PD) approach to investigate propofol free plasma levels in patients undergoing coronary artery bypass grafting (CABG) under hypothermic conditions (CPB-H) or off-pump CABG (OPCAB). Materials and methods: Nineteen patients were scheduled for on-pump coronary artery bypass grafting (CABG-CPB, n=10) or off-pump coronary artery bypass grafting (OPCABG, n=9) were anesthetized with sufentanil and propofol TCI (2 μg/mL). Blood samples were collected for drug plasma measurements and BIS were applied to access the depth of hypnosis from the induction of anesthesia up to 12 hours after the end of propofol infusion, at predetermined intervals. Plasma drug concentrations were measured using high-performance liquid chromatography, followed by a propofol pharmacokinetic analysis based on two compartment open model, PK Solutions v.2; PK-PD analysis was performed by applying EMAX model, sigmoid shape-variable slope and data were analyzed using Prisma v. 5.0, considering p<0.05 as significant difference between groups. Results and conclusion: Pharmacokinetics of free propofol in CPB-H group compared to OPCAB group based on two compartment open model was significantly different by the prolongation of distribution half-life, increases on plasma clearance, and biological half-life shortened. In addition, the kinetic disposition of propofol changes in a different manner considering free drug levels in the CPB-H group against OPCAB group as follows: prolongation of distribution half-life and increases on volume of distribution, remaining unchanged biological half-life in spite of plasma clearance increased. BIS values showed a strong correlation with free drug levels (r2>0.90, P<0.001) in CPB-H group and also in OPCAB group by the chosen EMAX model sigmoid shape-variable slope. Financial support or fellowship: FAPESP CFSP143 PHARMACOTHERAPEUTIC ESCORT FOR SENIOR CITIZEN PATIENTS FROM A NEEDY COMMUNITY IN MOGI DAS CRUZES - SP MARTA VERÔNICA DA SILVA QUEIROZ NASCIMENTO1; LETICIA FONTANA TORRAGA2; LUIS CARLOS DA SILVA3; JULIANA SILVEIRA PAIVA2. Pharmacist Care League Janaína Oliveira, University Of Mogi das Cruzes, Suzano - SP - Brazil; 2Pharmacist Care League Janaína Oliveira, University Of Mogi das Cruzes, Mogi das Cruzes - SP - Brazil; 3Pharmacist Care League Janaína Oliveira, University Of Mogi das Cruzes, Caçapava - SP - Brazil. 1

Introduction and objective: Through the years, the Brazilian population’s life expectancy has grown and with that, the incidence of disorders and health issues has grown too. The use of several medicines in this population causes modifications in pharmacokinetics and pharmacodynamics, which may cause a negative resonance in the elderly individual’s health. Therefore, the objective of this work was to provide assistance and pharmaceutical attention with pharmacotherapeutic escort to a senior needy female community in Mogi das Cruzes in a period between May and July, 2017. Materials and methods: The study group was composed of 14 female members aged over 65 years old. A pharmacotherapeutic escort of the group was set up, making use of pharmaceutical interviews adapted to Dáder’s methodology. A blood glucose and blood pressure monitoring was also performed, and possible Problems Related to Medicine Use (PRM) investigated. To accomplish the drug study “Micromedex® Solutions” software it was used. UMC’s ethical committee approved the study, certificate number 2.045.867/201. Results and conclusion: In 71.43% of cases, polypharmacy (use of 5 or more medicines) was evidenced; the quantity of medicines per patient was 6.57% ± 3.25 and by use of active ingredient was 7.57±3.62. Hypertension (29.55%), hypercholesterolemia (11.36%), diabetes Mellitus (11.36%), hypothyroidism (6.82%) and arthrosis (6.82%) were the most frequent found pathologies. Levothyroxine Sodium (5.66%) was the most common active ingredient prescribed. Inefficient quantity PRM was identified in two patients of the study group causing a pharmaceutical intervention and performing a routing report to a responsible physician. Financial support or fellowship: UNIVERSITY OF MOGI DAS CRUZES (UMC).

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CFSP144 PHARMACOTHERAPEUTIC EVALUATION OF HYPERTENSIVE PATIENTS ATTENDED IN AN UNIVERSITY OUTPATIENT CLINIC LUCIANA VISMARI1; VERA LUCIA DE CAMPOS ALMEIDA1; EVELYN BARBOSA GIGLIOLI1; NORMACELIA SOLEDADE DA SILVA1; MARCOS ROBERTO BIASOLI1; ANA CRISTINA FERREIRA DE JESUS1; FELIPE MATIAS DA SILVA1; CLEODENCE SANTANA NASCIMENTO1; FERNANDA MAEDA CARNEIRO1; EDIJENÁRIA PEREIRA REIS1; JOSIANE MOTTA E MOTTA1; SILVANA CARDOSO BASTOS1; ADRIANA FERREIRA GROSSO1; HENO FERREIRA LOPES2. Uninove, São Paulo - SP - Brazil; 2Uninove/Incor, São Paulo - SP - Brazil.

Introduction and objective: The greatest barrier found to effective control of the arterial hypertension is low patient adherence, often associated with polypharmacy. Thus, the objective of this study was to evaluate hypertensive patients in order to identify possible pharmaceutical diagnoses, to establish interventions, aiming at solving the problems encountered. Materials and methods: Non-controlled hypertensive patients, ≥18 years old, attended in the Primary Care Integrated Ambulatory from UNINOVE participated. After reading and signing the Informed Consent Form (ICF) and an initial interview, non-adherent patients were randomized in two groups: with and without pharmaceutical follow up. Pharmacotherapeutic evaluation were performed according to the Third Consensus of Granada (Dader Method). Results and conclusion: 31 patients were enrolled in the study, 26 of them were non-adherent. Nine non-adherent agreed to be followed by the pharmaceutical care and one adherent was invited, aged 63.8 ± 10.8 years. The number of drugs/person was 4.6 ± 1.95, of which 2.1 ± 0.9 antihypertensives. There were 3±1.6 Negative Outcomes Associated with Medication/person, 12 problems/risks of insecurity, 9 of ineffectiveness and 8 of need. The pharmaceutical intervention included strategies to increase adherence, education in health and contact with the prescriber. These preliminary results suggest the importance of the pharmaceutical follow-up within a multidisciplinary team to the success in controlling hypertension. Financial support or fellowship: UNINOVE CFSP145 PHARMACOTHERAPEUTIC FOLLOW UP AND DRUG INTERACTIONS IN THE HOMECARE SETTING IN THE MUNICIPALITY OF MOGI GUAÇU, STATE OF SÃO PAULO, BRAZIL ALEXSANDRO GONCALVES DE MORAES1; SUELEN KARINA MINELI JUSTINO2; NÁDIA REGINA BURIM ZUIM2; CAMILA STÉFANI ESTANCIAL FERNANDES2; RENATA LOPES RODRIGUES2; DANYELLE CRISTINE MARINI2. Fimi, Mogi Mirim - SP - Brazil; 2Fimi, Mogi Guaçu - SP - Brazil.

Introduction and objective: Patients who are unable to visit primary care services and require home care often take several medications to maintain their health, which constitutes polypharmacy. However, such patients do not always have family caregivers who are correctly oriented with regard to the use of the medications, which increases the risk of adverse drug interactions that can hinder the healing process and exert a negative impact on the quality of life of the patient. Thus, the Brazilian Ministry of Health created the Better Home Care Program, which determines the de-institutionalization of patients that can be treated at home, with frequent visits by multidisciplinary teams. The aim of the present study was to evaluate the drug therapies of patients followed up in the home setting. Materials and methods: A descriptive, cross-sectional study was conducted following approval from the local ethics committee (certificate number: 43825115.0.0000.5679). Twenty patients registered with the Home Care Service of the municipality of Mogi Guaçu, state of São Paulo, Brazil, were evaluated using an adaptation of the Dáder Method. Results and conclusion: Severe drug interactions and a lack of health professionals to offer care for this population were found, which renders pharmaceutical interventions with patients impossible, as the majority do not have medical follow up. The findings underscore the need to reformulate the home care program, emphasizing the extreme importance of pharmacists to enable the establishment of adequate measures that address the real needs of patients and their caregivers.

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CFSP146 PHARMACOTHERAPEUTIC FOLLOW-UP OF PATIENTS IN TREATMENT INVOLVING PSYCHOTROPIC MEDICATIONS MARINA INÁCIO BERTOLOTO1; DANYELLE CRISTINE MARINI2. Unifae, Vargem Grande do Sul - SP - Brazil; 2Unifae, Mogi Guaçu - SP - Brazil.

Introduction and objective: Pharmacotherapeutic follow up comprises a continual cycle of activities with the aim of resolving and preventing problems related to the use of medications and ensuring the safest, most effective drug therapy. The aim of the present study was to conduct pharmacotherapeutic follow up of 20 patients taking psychotropic medications that act on the central nervous system and evaluate potential drug interactions as well as the risks and consequences of each medication used. Materials and methods: A descriptive, cross-sectional study was conducted following approval from the local ethics committee (certificate number: 61721116.5.0000.5382). Twenty patients were evaluated with the aid of a care plan involving the SOAP note documentation method to determine the socioeconomic profile, verify the medications used, analyze medication-related problems, and perform pharmacotherapeutic follow up. Results and conclusion: Twenty individuals (18 females and two males) were evaluated. Ten different medication-related problems were determined based on an analysis of the medications and patient complaints. A plan was subsequently established individually for each patient. For cases in which there was a need to alter the prescription, a letter was sent to the prescribing physician requesting a review of the case. The best way to monitor the results of the implemented plan was determined jointly, especially for cases in which changes occurred in the prescription of the medication or clinical status of the patient, thereby establishing the cycle of pharmacotherapeutic follow-up. CFSP147 PHARMACOTHERAPEUTIC FOLLOW-UP IN THE USE OF NITRAZEPAM FOR A CHILD IN THE PEDIATRIC INTENSIVE THERAPY UNIT OF A TEACHING HOSPITAL BRENA GEYSE MESQUITA ROCHA SOARES1; PAULO SÉRGIO CORRÊA SIEBRA1; MARIA ALANA DUARTE MARINHO1; ANTONIA RAFAELLA FERREIRA GOMES MARTINS1; ADNA VASCONCELOS FONTELES1; NAYARA ARAÚJO CARDOSO2; ANTONIO ERIVELTON PASSOS FONTENELE1. Santa Casa de Misericórdia de Sobral, Sobral - CE - Brazil; 2Universidade Federal do Ceará, Sobral - CE - Brazil.

Introduction and objective: In pediatrics, it is common to use off-label drugs and pharmaceutical forms that are not recommended for specific age groups, and a pharmacotherapeutic follow-up is necessary to detect possible drug-related problems (PRM) and to prevent side effects associated with the off-label drug. Therefore, the objective of this study was to report the pharmacotherapeutic follow-up of a child taking nitrazepam. Materials and methods: The pediatric intensive care unit of the Santa Casa de Misericórdia Hospital of Sobral was evaluated by the pharmacist in March 2017 using the Subjective, Objective, Evaluation, and Plan method. The present study was governed by the ethical principles of Resolution 510/2016 of the National Health Council. Results and conclusion: The use of half of the 5 mg tablet form of nitrazepam was given by the pharmacist to a child 6 years of age, while the Epilepsy Society recommends the use of nitrazepam for children.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP148 PHARMACOTHERAPEUTICAL FOLLOW-UP OF CHRONIC NON-TRANSMISSIBLE DISEASES BY DIFFERENT CARE STRATEGIES AT THE PRIMARY HEALTH CARE NETWORK OF VÁRZEA PAULISTA - SP. VERONICA CRISTINA GOMES SOARES1; VANESSA DE OLIVEIRA ALBUQUERQUE2; SABRINA DE ALMEIDA MARQUES 2; APARECIDA ERICA BIGHETTI RIBAS 2; NATÁLIA CASTANHA DA SILVA 2; HEDER FRANK GIANOTTO ESTRELA2. UNIP, Jundiaí - SP - Brazil; 2Unianchieta, Jundiaí - SP - Brazil.

Introduction and objective: Chronic Non-Transmissible Diseases (CNTD) are a major concern in the field of public health around the world. These diseases stand out among the priorities of the Unified Health System (UHS), where Primary Care teams in every country develop care strategies for their patients, aiming at metabolic control, reduction of complications, and success of drug treatment. The objective of the study was to determine whether different strategies of patient management of three Basic Health Units (BHUs) of Várzea Paulista-SP, especially the performance of the pharmacist, presented financial impact on spending on CNTD. Materials and methods: Through analysis of public data, such as the number of physicians, number of consultations offered, development of orientation actions, it was possible to perceive that these differences impact on the pharmacotherapeutic follow-up of three diseases that include the role of non-transmissible chronic diseases: Diabetes mellitus, Systemic Arterial Hypertension and Dyslipidemias. The data were collected in the SUSs public information system, no patient or professional was interviewed. The results were tabulated in Excel for analysis. Results and conclusion: In the city of Várzea Paulista-SP, primary care is marked by heterogeneity, since each Basic Health Unit (BHU) has structural and managerial differences. Comparing three BHUs with correlated populations, but with large differences related to care, impacts related to the quantity of medicines and their respective expenses. With analysis of the results it was evident that the non-presence of pharmacists in 1 of the BHUs reflected in a treatment cost of CNTDs of R$23.54 more per patient. It was concluded that the pharmacist’s lack of effective intervention in the care strategies leads to financial losses and the quality of the treatment made available to the patient.

PHARMACOTHERAPEUTICAL MONITORING THROUGH DADER METHOD APPLICATION

CFSP149

EVERTON BOFF 1; ALANA CARLA BATTISTELLA 1; RÉGIS CARLOS BENVENUTTI 1; VANESSA DA SILVA CORRALO2. Unoesc, São Miguel do Oeste - SC - Brazil; 2Unochapecó, Chapecó - SC - Brazil.

Introduction and objective: Chronic diseases are responsible for the highest morbidity and mortality rates in Brazil and for highest proportion of outpatient and hospital care expenses. They cause a substantial health burden, cause economic impact and deteriorate the quality of life for people, families and communities. The objective was to evaluate the efficacy of pharmaceutical guidance services for chronic drug users in the western region of Santa Catarina. Materials and methods: The study encompassed 117 chronic patients living in the western region of Santa Catarina. It was a quantitative, qualitative and transversal study, with an exploratory character. It was approved by the Ethics Committee, with certificate number 1.966.071 and was carried out through interviews according to the Dáder method. Afterwards, the level of knowledge of the patient about the medication and the degree of adherence to the therapy were detected. Next step was to obtain necessary information about health problems and medication. Then, the real needs of medicines, effectiveness, safety and professional negligence were investigated. Finally, the intervention was carried out, where realistic priorities and goals achievable to the patient were established. Results and conclusion: Of the 117 interviewees, 63% were women, overweight and over 41 years old. It was also observed that the number of users who do not have their diseased controlled is high, with a large number of people consuming 5 or more medications daily, increasing the chances of an error at the time of ingestion.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

PHARMACOVIGILANCE AND PRIMARY CARE: THE SEARCH FOR THE SAFE USE OF DRUGS

CFSP150

JULIANA RIBEIRO IBIAPINA1; ANA PAULA PESSOA MACIEL1; MAYARA ALCANTARA DE ALBUQUERQUE2; RENAN PEREIRA DE LIMA1; ARLANDIA CRISTINA LIMA NOBRE DE MORAES1. Universidade de Fortaleza, Fortaleza - CE - Brazil; 2Universidade de Fortaleza- Unifor, Fortaleza - Brazil.

Introduction and objective: The use of drugs, even at therapeutic doses, can cause unpleasant effects to the patient. The term pharmacovigilance was defined by the World Health Organization as “the pharmacological science relating to the collection, detection, assessment, monitoring, and prevention of adverse effects with pharmaceutical products”. The practice of pharmacovigilance is essential to achieving rational and safe use of pharmaceutical products. Nevertheless, it is possible to see that primary care is still lacking in this aspect. Thus, this study had the objective of analyzing the profile of Adverse Effects (AE) found at a Basic Care Unit for a period of 9 months. It was done in done in order to sensitize the primary care professionals about the importance of notifications. Materials and methods: The study took place at a Basic Care Unit (BCA) in Fortaleza. It belongs to the Executive Secretariat Regional VI. It is an observational, descriptive and prospective study. The data was collected through an active search of patients and through the spontaneous notifications of professionals. The statistical analysis of the data was done according to the frequency (n) and the percentage (%). Results and conclusion: 23 AE notifications were made, most resulting from the active search. There was a greater incidence of mild reactions, possible and from type A (predictable). The small number of spontaneous notifications highlights the need of more involvement from professionals, disseminating the idea that safe use of drugs will bring a better quality of life to the population and will reduce the morbimortality related to drugs.

PHARMACOVIGILANCE IN PHARMACIES AND DRUGSTORE: CURRENT SITUATION

CFSP151

NICOLE ANDRESSA CANIBAL; BRUNA ARAÚJO MARTINS FIRMINO; JOÃO CEZAR CASTILHO. Faculdade de Jaguariúna, Jaguariuna - SP - Brazil. Introduction and objective: The importance of pharmacovigilance should be promoted, emphasizing the fact that the life of a drug actually affects its marketing. Our study aimed to evaluate the frequency of adverse events (AEs) reported by patients, the conduct of the professional pharmacist faced with this situation, professional knowledge regarding the pharmacovigilance and their perception about health agencies in Brazil. Materials and methods: A field study, analytical and observational, carried out from April to May 2016. Sixty one pharmacists of pharmacies and drugstores, included randomly, were interviewed in the municipalities of Campinas-SP, Jaguariuna-SP, Monte Siao-MG and region. Sixty pharmacists completed the questionnaire and one asked to interrupt the research. The study was conducted by applying a standard questionnaire to professionals. Approved by the Research Ethics Committee of the Faculty of Jaguariúna (No. 1.470.516). Results and conclusion: 54% of establishments were registered in ANVISA’s notification system. The results showed that 73% of pharmacists received AEs reports. Of these, 82% of the professionals would advise the patient to consult a doctor and 27% would recommend the patient to contact the manufactures of the drugs. Few professionals report these AEs to ANVISA or CVSSP (10% and 5%, respectively). The majority of pharmacists attributed the patient’s AEs reports as “very important/important” (95%). While 88% reported being “very important/important” reporting these suspected AEs to ANVISA, CVS-SP and the manufacturer. This study suggest that policies and programs are necessary to raise awareness of the importance of the pharmacist in the post-marketing surveillance, assuming their role in the receipt, evaluation and, as the case, notification of these events with ANVISA, CVS-SP and/or manufacturers. In this way, the pharmacist can actively contribute to the efficacy and safety drugs profiles available in the therapeutic arsenal of Brazil.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP152 PHYTOTHERAPY DAY: PHARMACEUTICAL EDUCATION IN THE PROMOTION OF RATIONAL USE OF MEDICINAL PLANTS LUCAS BARBOSA TEIXEIRA; JAMILLE SILVEIRA MARTINS; THAIS SIQUEIRA BACELAR; FRANCISCO ANGELIM DE SOUSA; ALDO JOSÉ DA COSTA LOPES; MARIA AMANDA DOS SANTOS FREITAS; TAIANA MAGALHÃES PIERDONÁ; SUZANA BARBOSA BEZERRA. Fametro, Fortaleza - CE - Brazil. Introduction and objective: The Live Pharmacies Project (LPP) works as a pharmaceutical social assistance program, based on WHO recommendations, with emphasis on actions directed toward primary health care. The program provides the community with medicinal plants (MP) with proven pharmacological properties and guidance for their recognition. In view of this, the objective of this work was to create the 1st Phytotherapy Day of Fametro with the intention of promoting pharmaceutical education in the Phytotherapy area. Materials and methods: The work carried out was an educational event developed by students of the Pharmacobotanical discipline in the Pharmacy Course and teachers. Thus, it was able to make some educational materials from MP species certified and registered in the LPP and cultivated in the Herb Garden of Fametro (Lippia Alba, Cymbopogon winterianus, Cymbopogon citratus, Justicia pectoralis, and others) and elaboration of innovative homemade preparations. Results and conclusion: During the day, students demonstrated to the public the main effects of natural products and the malfunctions of its improper use. Innovative pharmaceutical preparations have also been created such as Justicia pectoralis candy for infant respiratory conditions, natural cosmetics from Aloe vera for skin hydration, among others. The model of the journey created is compatible with the norms of the national policy of herbal and phytomedicine approved by the Federal Government and its adoption could be used by other institutions to promote the rational use of medicinal plants by the community.

POLYPHARMACY IN MARAVILHA\SC

CFSP153

RÉGIS CARLOS BENVENUTTI1; ELISANGELA DA SILVA LOPES2; EVERTON BOFF1; VANESSA DA SILVA CORRALO3. Unoesc e Unochapecó, São Miguel do Oeste e Chapecó - SC - Brasil; 2Unoesc, São Miguel do Oeste - SC - Brasil; 3Unochapecó, Chapecó - SC - Brazil. 1

Introduction and objective: The progressive aging of the population results in associated and continuous use of medications; polypharmacy. This practice is potentially harmful considering that drug interactions generate intoxication, annulment of the therapeutic effect, non-treatment of the disease or its symptoms and exacerbation of adverse reactions. This study aimed to investigate the use of drugs among elderly people living in Maravilha/SC. Materials and methods: The study was approved by the Research Ethics Committee (CEP) certificate number 2040273. We interviewed 100 elderly people, evaluating socioeconomic aspects, the number of drugs consumed, the most frequent indications and the time of use. Results and conclusion: Of the interviewees, 82% were female and 68% did not work in the labor market. Most of the informants had a health problem (71%) and 82% more than one medication for continuous use, especially antihypertensive drugs (51%). Investigating the time of use, it was noticed that 25% had used the drugs for more than 10 years. It was concluded that polypharmacy is present in the daily life of elderly people, indicating a high incidence of symptoms, consultations and treatments with multiple specialists, easy access to medicines and low frequency of non-pharmacological resources.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP154 POTENTIAL DRUG-DRUG INTERACTIONS BETWEEN PRESCRIBED AND NON-PRESCRIBED MEDICINES USED BY HEMODIALYTIC CHRONIC RENAL PATIENTS NARA JACQUELINE SOUZA DOS SANTOS1; IONARA VIEIRA ROCHA DA MOTA1; ADRIANA ALVES NERY2; LUCAS BRASILEIRO LEMOS2; GISELE DA SILVEIRA LEMOS3. *Department of Science and Technology, State University of Southwest Bahia (Susb), Jequié - BA - Brazil; 2**Department of Health Ii, State University of Southwest Bahia, Jequié - BA - Brazil; 3*Department of Science and Technology, State University of Southwest Bahia (Susb), Jequié - Brazil. 1

Introduction and objective: The treatment of chronic kidney disease (CKD) involves the use of large quantities of drugs, with the possibility of potential drug-drug interactions (PDDI), so that the risk of PDDI increases with self-medication. The objective of this study is to evaluate the PDDI between prescribed and non-prescribed drugs in patients with CKD in a hemodialysis clinic. Materials and methods: A cross-sectional study with a form applied to 170 patients from a hemodialysis clinic during the period from March to May of 2015. The analysis of the PDDI was performed using the MICROMEDEX® version 2.0 database. The project was approved by the Research Ethics Committee of SUSB (certificate No. 957.553). Results and conclusion: The number of patients in whom it was possible to verify the use of prescribed drugs associated with those of the non-prescribed ones was 51. The mean number of PDDI per patient using non-prescription drugs was 0.63 (SD ± 1.09). The drugs most associated to IMPs were nimesulide (11.67%), dipyrone (10%), losartan (8.33%), clonidine (8.33%), propanolol (6.67%), captopril (6.67%), enalapril (6.67%), AAS (6.67%), atenolol (5%), digoxin (3.33%) and others (26.7%). Analysis of the interactions between prescription drugs and those used in self-medication has great capacity to support the professionals involved in the follow-up of these patients to ensure greater safety and effectiveness in drug treatment. Self-medication is generally neglected by prescribers. Financial support or fellowship: SUSB

POTENTIALLY DANGEROUS MEDICINE: THE RISKS OF USE IN CHILDREN

CFSP155

MAYARA ALCANTARA DE ALBUQUERQUE1; CESIANY MONIQUE DIAS CAMPOS2; RENAN PEREIRA DE LIMA2; ARLANDIA CRISTINA LIMA NOBRE DE MORAES2; GEYSA AGUIAR ROMEU2. Universidade de Fortaleza- Unifor, Fortaleza - CE - Brazil; 2Universidade de Fortaleza, Fortaleza - CE - Brazil.

Introduction and objective: Potentially Dangerous Medicines (PDM) are thus called because they present a high risk of causing adverse reactions in case of erroneous usage. Children are considered therapeutic orphans for two main reasons. The first is that they are not included in new medication clinical trials. The second is that they do not have a completely developed physiological structure. The pharmacist is usually the last health professional to get in direct contact with the patient after the doctor takes the decision to go with pharmacotherapy. This study intends to identify and describe which PDMs are the most prescribed at a Pediatric Intensive Care Unit (PICU). The author also intends to help the rational use of medicine by highlighting the importance of having a pharmacist in the multidisciplinary team. Materials and methods: This is a prospective, transversal and observational study. In it all duplicates of prescriptions received at the dispensation from a large hospital drugstore in Fortaleza, which came from the pediatric ICU and showed one or more PDMs were analyzed. This study was approved by the Research Ethics Committee Involving Human Beings at the “Universade de Fortaleza” under certificate No. 277/10. Results and conclusion: During the period of this study, it was verified that of a total of 522 prescriptions received 23.2% had one or more PDMs. The most prescribed PDM was Fentanyl. It was prescribed 53 times which adds up to 10.2% of the analyzed prescriptions. After that, the most prescribed PDMs were, in order, Midazolam, Calcium Gluconate, Potassium Clorite, Dobutamine and Insulin. This study showed that there is a possibility to subsidize the making of lists, which will have high priority drugs to the progress of medical research in the parts of the population yet to be studied, showing thus the need to focus in this area.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP156 POTENTIALLY INAPPROPRIATE MEDICATION USE IN OLDER ADULTS AND EMERGENCY DEPARTMENT VISITS AFTER AN ACUTE CONDITION STÉPHANIE DE SOUZA COSTA VIANA; NATÁLIA PEREIRA DOS SANTOS SOUZA; MÁRLON JULIANO ROMERO ALIBERTI; WILSON JACOB FILHO. Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo - SP - Brazil. Introduction and objective: Aging is accompanied by concerns related to non-communicable chronic diseases and clinical consequences of polypharmacy due to the physiological modifications of senescence, that make them increasingly vulnerable to adverse effects of medications use. The Beers Criteria were created to standardize the Potentially Inappropriate Medications (PIM) in older adults, due to the higher risk of falls, functionality reduction, institutionalization, hospitalization, and death. The aim of this study is to analyze the association between PIM use and emergency department (ED) visits after an acute condition treated in a geriatric day hospital (GDH). Materials and methods: On admission, a comprehensive geriatric assessment was performed to evaluate participants. Then, they were followed-up for one year by monthly telephone contact to detect the occurrence of ED visits. Cox proportional regression model adjusted for age, sex, race, income, multimorbidity, the number of drugs in use, cognition, depression, and functionality analyzed the association of PIM use at baseline with incident ED visits. Results and conclusion: 534 older adults were included (mean age of 79.6 [±8.4] years old, 63.5% female). The prevalence of PIM use was 43.8%. The most prevalent ones were proton pump inhibitor (31.3%), antidepressants (5.6%), and muscle relaxants (5.0%). During the one-year follow-up period, 363 (68.0%) older adults visited the emergency department. Participants who used PIM at baseline showed a significantly increased risk of future ED visits (hazard ratio 1.29, 95% confidence interval 1.02–1.62, p=0.03). The use of PIM according to 2015 Beers Criteria was significantly associated with the incidence of ED visits. Additionally, an approach of reviewing the drugs used by older adults in this scenario could have a positive impact on the evolution of these patients. Financial support or fellowship: Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES). CFSP157 PRESCRIPTION INDICATORS STUDY: A METHOD IN PHARMACEUTICAL CARE FOR AT RISK PREGNANT WOMEN SANDNA LARISSA FREITAS DOS SANTOS1; HÉRICK HERBET DA SILVA ALVES2; ATHILA WESLEY LIMA LACERDA1; NATALHA NAYANE DE OLIVEIRA PINHEIRO1; KARLA BRUNA NOGUEIRA TORRES BARROS2. Universidade Federal do Ceará, Fortaleza - CE - Brazil; 2Centro Universitário Católica de Quixadá, Quixadá - CE - Brazil.

Introduction and objective: Based on Rational Use of Medicines, the World Health Organization (WHO) has developed indicators of drug use as a way to safely describe and evaluate aspects that affect pharmaceutical practice. Objective: To verify the prescription indicators described by WHO, focusing on Pharmaceutical Care for high-risk pregnant women attended at the Francisco Carlos Cavalcante Roque Clinic Center in Quixadá, Ceará. Materials and methods: It was a cross-sectional study with a quantitative method. Prescriptions of 80 pregnant women were collected and analyzed in the period from March to May, 2016. Prescription indicators were analyzed according to the How to Investigate Drug Use manual in WHO’s Health Facilities Results and conclusion: Most of the pregnant women belonged to the 29 - 39 age group (60%) and were married (67.5%). The predominant diseases were infection (22%) (Urinary, vaginal and intestinal), Hypertension (18%) and Anemia (14%). 99 drugs were observed in the prescriptions, with the most commonly prescribed methyldopa (20%), ferrous sulphate (11%) and cephalexin (8%). In the analysis of the prescription indicators an average of 1.3 drugs per prescription was found, 70% of drugs prescribed by generic name, 5.05% with at least one injectable drug, 13.1% of them containing antibiotics and 76.7% of prescription drugs based on the list of essential medicines of the municipality. Therefore, it is suggested that the prescriptions of medications for pregnant women be evaluated continuously in order to minimize the occurrence of adverse effects and health damage to the baby.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP158 PRESCRIPTION PHARMACEUTICALS IN PHYTOTHERAPY: ASSESSMENT OF CONDITIONS FOR ITS IMPLEMENTATION IN CUIABÁ – MT ELAINE MARTINS DE RESENDE1; REGINA MARA DE SOUZA PEREIRA2; LUIS CARLOS MARQUES3. Universidade Anhanguera de São Paulo, Cuiabá - MT - Brazil; 2Universidade Anhanguera de São Paulo, São Paulo - SP - Brazil; Fitoscience Consultoria Ltda., São Paulo - SP - Brazil.

1 3

Introduction and objective: In Brazil and worldwide the pharmaceutical profession has been undergoing changes in some aspects. The Federal Pharmacy Council (CFF) in turn, has been regulating the performance of this professional. So in August 2013, through Norm nº 586, pharmaceutical prescription was regulated as an additional service offered by this professional. The aim of this study was to evaluate the professional preparation of pharmacists in charge of Cuiaba’s drugstores in relation to CFF Norm 586, using phytotherapy as a specific object of evaluation. Materials and methods: A qualitative and quantitative cross-sectional study was carried out through an interview with the application of a questionnaire with open and closed questions in a sample of sixty-two pharmacists. Data were statistically assessed through the Chi-square test with significance p≤0.05. Results and conclusion: The majority of the 62 pharmacists interviewed were women (73%); predominantly between 26-31 years of age (37.1%); 1-5 years since graduation (87%), from 1-5 years’ experience as a pharmacist in drugstores (48%). Most of the interviewees know the CFF Norm nº 586 (85%), but do not prescribe allopathic (53.2%) or phytotherapic (50%) products. The pharmacists’ knowledge of phytotherapics and their interactions was considered insufficient (76%), median for 18% and good for 6%. Most of the research participants have a positive point of view about the CFF Norm nº 586, but do not show enough knowledge for the prescription of phytotherapics. There is urgent need for educational measures to improve the knowledge of the pharmacist about phytotherapeutics. One possible path is the implementation of the title of specialist in clinical phytotherapy in Brazil. Financial support or fellowship: UNIAN CFSP159 PRESENTATIONS OF PHYTOMEDICINE SALE AS WEIGHT CONTROL IN PHARMACIES IN JUNDIAÍ -SP VERONICA CRISTINA GOMES SOARES. UNIP, Jundiaí - Brazil. Introduction and objective: The use of herbal medicines to control obesity has shown a continuous growth, a fact that contributes to the research and the emergence of new products for the purpose of weight loss, such as: Cactus (Opuntia ficus), Green coffee (Coffea robusta) and African mango (Irvingia gabonensis). This study aims to determine which are the most frequently sold combinations of herbal medicines for weight loss, found in compounding pharmacies. This study aims to determine which phytotherapics are most frequently associated with Cactus, Green Coffee and African Mango, found in compounding pharmacies, for weight loss. Materials and methods: The study was carried out by means of a questionnaire containing 10 questions, approved by the Ethics Committee of the Padre Anchieta University Center nº 050145/2015, which was applied in compounding pharmacies in the municipality of Jundiaí-SP. Results and conclusion: The phytotherapics under study are frequently associated with: Citrus aurantium, Garcinia cambogia and phaseolamina, being dispensed in the pharmaceutical form of capsules, to a predominantly female public. Although natural herbs are not free of side effects, associations, although common, do not present enough studies, which can endanger the health of users. The phytotherapeutic associations although common in this case do not present sufficient studies for safe dispensation, described in scientific literature these associations can cause alterations of gastrointestinal tract. Further studies are needed for the safe dispensing of these associations.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

PREVALENCE OF ENTEROPASITOSIS IN THE CITY OF MACAPÁ-AP.

CFSP160

FABIANE DA SILVA BATISTA1; DÁVYLA DE PAULA2; MATEUS CARVALHO MIRANDA2; CAROLINA MIRANDA DE SOUSA LIMA2; MAYARA AMORAS TELES FUJISHIMA2; CAMILA COSTA GUERRA2; ADRIANY BARRETO2; FLÁVIA SILVA COSTA2; RAIMUNDO FERNANDES BENTO FILHO2. Instituto Macapaense do Melhor Ensino Superior - Immes, Macapá - AP - Brazil; 2Universidade Federal do Amapá, Macapá - AP - Brazil. 1

Introduction and objective: The enteroparasitosis are considered public health problems in national territory. These pathologies are connected directly to socioeconomic and environmental factors that together condition the health-disease process. To measure threat, studies of prevalence are required that can be utilized in governmental planning for prevention, promotion and health recovery. Objective: Verify the prevalence of enteroparasitosis diagnosed by the laboratories of the Basic Health Unities in the city of Macapá-AP. Materials and methods: The research was descriptive-analytic of the transversal prospective type and was developed based on fecal parasitological exams results (FPE), of the direct type, dispatched in four Basic Health Unities (BHU) of the 8 active unities in the study period, in the city of Macapá-AP, from June to August of 2011, totalizing 4,492 samples. Results and conclusion: The analysis demonstrate the incidence of 17 species of intestinal parasites highlighting nonpathogenic protozoa, but indicating oral-fecal contamination (Endolimax nana – 33% and Entamoeba coli – 16%), and pathogenic protozoa Entamoeba histolytica (18%) and Giardia lamblia (15%). Of the diagnosed helminths, Ascaris lumbricoides (6%) and Trichuris trichiura (4%) stand out, these values serve as indicators of the sanitary conditions of the city. The aforementioned values have average of 15.33; Standard deviation of 10.3473; Variance of 107.06868. Starting from that point, it can be concluded that the prevalence of endoparasitosis in the city of Macapá-AP was 97%, based on the diagnostics, which indicates a high prevalence that can be linked to the precariousness of city sanitary conditions, as well as lack of hygienic and sanitary education of the population, increasing the costs of basic health attention and the threat to the population. If more sensitive techniques were performed, the prevalence would probably have a higher value.

PREVALENCE OF DISEASES AND MEDICINES USED IN RESIDENTS OF THE CITY OF SANTOS/SP

CFSP161

BRUNA TRINDADE RIBEIRO DE ARAUJO; JANARA DE CAMARGO MATOS; LOURDES CONCEIÇÃO MARTINS. Universidade Católica de Santos, Santos - SP - Brazil. Introduction and objective: The municipality of Santos was chosen as the study area for having the largest port in Latin America, correlated to high road and maritime traffic. The prevalence of chronic disease, as well as its complications usually increase with age. The pharmacist, being the professional responsible for the medicine, becomes co-responsible for better adhesion to drug therapy of patients with consequent improvement of the level of health. His actions in this context include the correct use of medicines advice, identification of the main health problems in the community, becoming partner in implementation of health promotion. Objective: Analyze the prevalence of diseases and medicines used on residents of two areas in the city of Santos/SP. Materials and methods: A cross-sectional study was conducted through a self-applied questionnaire, using a probabilistic sample and a descriptive analysis was performed of all variables and two proportion test compared. The significant level was 5%. Results and conclusion: The average age was 51.07 (DP = 18.91); 144 (51.1%) are men and 138 (48.9%) are women (p>0.05), most of them were nonsmoking 206 (73.0%) Financial support or fellowship: CNPq; PIBIC

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

PREVALENCE OF DISLIPIDEMIAS IN THE MUNICIPALITY OF ALTO PARAÍSO RONDONIA

CFSP162

ALEX ROBSON BONIN AMARO; ENEIAS MARCELINO DA ROCHA; FABIA MARIA PEREIRA DE SÁ ZEFERINO; FERNANDA TORRES. Faar-Faculdades Associadas de Ariquemes, Ariquemes - RO - Brazil. Introduction and objective: Lipids are fundamental constituents of cell membranes, which play a crucial role in the synthesis of steroid hormones, in the formation of myelin, with subcellular organelle function and in the non-combustible cellular contribution. Through the early detection of asymptomatic people, who were identified with high cholesterol levels, it is possible to identify and modify this risk factor for coronary disease. The present study aimed to evaluate the relevance of dyslipidemias in the city of Alto Paraíso (State of Rondônia, in Brazil). As specific objectives: Investigate the prevalence of patients with dyslipidemias in a Laboratory of Clinical Analyses in Alto Paraiso - RO; To investigate the incidence of mortality determined by Cardiovascular Diseases in the period from 2010 to 2015 through the Municipal Department of Health of Alto Paraíso - RO; To emphasize the importance of the early identification of the lipid profile with high serum levels, and to point out its direct relation in the development of atherosclerosis, consequently the DCV. Materials and methods: Therefore, a retrospective field study with a documentary character was performed. The research was carried out in a Laboratory of Clinical Analyses, also in the Municipal Secretary of Health, located in Alto Paraíso, Rondônia. The study was performed with 280 analyzed medical records. However, 13 were excluded because they were not old enough for research. Results and conclusion: Of those that were not excluded, 127 presented some type of alteration of lipid metabolism (47.57%) and 140 were normolipidemic (52.43%). The cardiovascular risk was prevalent for the male gender, calculated by the Castelli Indexes, which proves the results obtained, in which they are high. Despite the limitations (lack of information such as alcoholic and smoker habits, sedentary lifestyle, hypertension, body mass index and diet), this research made possible the construction of an epidemiological profile that showed lipid metabolism disorders, alerting the population about the need for measures to promote health and prevent cardiovascular diseases. CFSP163 PREVALENCE OF POTENTIAL DRUG-DRUG INTERACTIONS IN A BRAZILIAN UNIVERSITY HOSPITAL FRANCIS MAURO PEDROSO1; LUIS CARLOS MARQUES2; HÉLDER CÁSSIO DE OLIVEIRA3. Universidade Anhanguera de São Paulo, Cuiabá - MT - Brazil; 2Fitoscience Consultoria Ltda., São Paulo - SP - Brazil; 3Hospital Universitário Júlio Muller, Cuiabá - MT - Brazil. 1

Introduction and objective: Drug-drug interactions (DDIs) are significantly more likely to occur in hospital settings, where patients are commonly on multiple drug regimens, and they may lead to adverse clinical consequences. The aim of this study was to assess potential DDIs at the internal medicine ward of a university hospital located in Cuiabá - MT. Materials and methods: A descriptive, retrospective study was conducted on patients treated in the internal medicine ward of the University Hospital Julio Muller from January, 07, 2013 to October, 30, 2013. The drugs prescribed to patients were screened for potential DDIs using Micromedex® Healthcare Series software which classifies pairs of drugs with potential interactions according to the clinical significance based on the severity and extent of documentation. Results and conclusion: A total number of 2,639 medical charts were analyzed. The 275 patients (58.2% male, average length of stay 13 days) were prescribed a total of 17,327 items during hospitalization, and the average number of drugs per chart was found to be 6.5. Of the 222 prescribed drugs, the most common therapeutic class was antibiotics (18.1%), followed by antihypertensive (14.6%), non-opioid analgesics (10.2%) and proton pump inhibitors (9.9%). Of all the patients 67.6% had at least one potential DDI regardless of type of severity. A total number of 368 types of interacting combinations were identified, an average of 1.9 potential drug interactions per chart. Of all types of potential DDIs 60.3% were of moderated, 35.3% of major and 3.8% of minor severity, and two DDIs (0.6%) involved drugs whose combination was contraindicated. Among major interactions 70% have a good or excellent level of evidence, and omeprazole, phenytoin, tramadol, digoxin and warfarin were the drugs most commonly encountered. The results show that clinically significant DDIs are common during hospitalization, therefore identifying and preventing potentially harmful DDIs is a critical component of patient safety. Financial support or fellowship: UNIAN

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP164 PREVALENCE OF STAPHYLOCOCCUS AUREUS IN HOSPITAL INFECTIONS: IN VITRO DIAGNOSIS OF A VEGETAL EXCHANGE AND THE CONTROL OF MULTIRESISTANT STRAINS. AFONSO RAFAEL NASCIMENTO E SILVA1; CHRISTIAN NERI LAMEIRA2; INGRID SILVA DE OLIVEIRA2; MARIA JOSÉ SANTOS DE QUEIROZ2; SANT CLAIR SILVA DOS SANTOS2; DANILA TERESA VALERIANO ALVES2. Faculdade Integrada Brasil Amazônia, Belém - PA - Brazil; 2????????.

Introduction and objective: Research demonstrates that there is a relationship between microbial resistance and indiscriminate use of antibiotics. The epidemiology of hospital infections reveals that S. aureus presents a higher index among the etiological agents. Studies present the sensitivity of S. aureus to the extracts of Punica granatum, Lippia sidoides and Quale grandiflora. Objectives: The objective of this study was to analyze the in vitro antibacterial susceptibility profile of a plant exudate against the gram positive S. aureus microorganism. Materials and methods: The research was carried out evaluating the bactericidal profile of the exudate and its inhibitory capacity on S. aureus. The bactericidal profile of Copaifera sp oleoresin at concentrations of 0.5%, 2.5%, 5.0%, 7.5% and 10.0% was evaluated against gram positive strains S. aureus ATCC 25923 using the dilution technique in Müller Hinton agar medium. Results and conclusion: The results showed that at the concentration of 0.5% there were significant values for the bacteria, while at 10% there was total inhibition of the microorganisms where the oil of copaíba showed the inhibitory capacity of growth of Escherichia coli, S. aureus and Pseudomonas aeruginosa. The exudate was effective against the multidrug resistant pathogen in spite of having a positive result, a detailed study with greater clarification regarding the variations of results involving the genus Staphylococcus, methods, concentrations and chemical components in the plant is necessary. However, this result supports its possible rational application in phytotherapy and in combating bacterial multiresistance. CFSP165 PREVALENCE OF THE USE OF TOPICAL NASAL VASOCONSTRICTOR DECONGESTANTS AMONG UNIVERSITY STUDENTS FROM BAIXADA SANTISTA WESLEY BRAS DOS SANTOS; DANIELA MARTINS DA SILVA. Universidade Paulista, Santos - SP - Brazil. Introduction and objective: Respiratory diseases are one of the main causes of self-medication in Brazil. Rhinitis affects about 25% of adults in Brazil, and among the various subtypes of rhinitis is drug rhinitis, due to the chronic use of topical nasal vasoconstrictor decongestants. These drugs promote a change in nasal physiology causing a rebound effect. Objective: To evaluate the prevalence of vasoconstrictor topical nasal decongestants use by university students as well as their risks, dependencies and the way in which they obtained these medications. Materials and methods: 400 students were interviewed during the first semester of 2017 near the universities of Baixada Santista, these students were divided between health area and exact sciences. The questions of the interview were composed of questions that could give clarity on the acute and chronic use of these substances. Results and conclusion: The total sample was of 400 students. About 239 students (59.75%) reported having used some type of nasal vasoconstrictor decongestant. Of the 239 students who said they had already used one, 120 students belong to the area of health and 119 students belong to the area of exact sciences. There was no significant difference between the two areas (p = 0.91). Among students who have ever used the drug, 134 students (56%) said they had used it for less than a week, about 43 students (18%) said they had been using it for more than a year. About 168 students (70.3%) of the 239 students who have ever used, obtained the medicine without consulting a health professional. In the study, the prevalence of these drugs was observed in the students. Most of the students obtained the drug without consulting a health professional and used it for a longer period than recommended, demonstrating a high need to raise awareness of the risks of these drugs in their daily lives. Financial support or fellowship: Vice-rectory of Post-Graduation and Research at the Paulista University UNIP.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP166 PROFILE OF CERVICAL CANCER MORTALITY IN BRAZIL: EPIDEMIOLOGICAL DATA FROM 2000 TO 2013 FABIA MARIA PEREIRA DE SĂ ZEFERINO; ROSICLER BALDUINO NOGUEIRA; ENEIAS MARCELINO DA ROCHA. Faar-Faculdades Associadas de Ariquemes, Ariquemes - RO - Brazil. Introduction and objective: Cervical cancer, not including non-melanoma skin cancers, according to DATASUS (2013), is the second most prevalent type of cancer among women in Brazil, losing only to breast cancer, the consensus in the literature being that the etiologic agent that causes this pathology is the Human Papillomavirus (HPV), present in more than 95% of malignant tumors in the cervix. Thus, the objective of this study was to study the rates of cervical cancer mortality in Brazil, between 2000 and 2013, for this purpose, the data was searched in the DATASUS platform of the Ministry of Health. Materials and methods: The collection of epidemiological data was performed in the DATASUS platform, which is a tool developed by the Department of Information Technology of the Unified Health System created to supply the Unified Health System (SUS), with data on the main health indicators in Brazil. Important to subsidize actions related to the Health System. The search included data on mortality related to cervical cancer in Brazil, using the time period between 2000 and 2013. The mortality data surveyed involved the relationship between cancer and its prevalence by age, locality and time interval. Results and conclusion: In relation to cervical cancer mortality, death rates represent about 1.0% of all deaths in women in Brazil, with the highest rates in the North and Northeast regions. Higher rates in this period of time studied. Regarding the age group in which the highest mortality occurs, one was the age of 40 to 69, about 15 to 20 years after the first sexual intercourse. In addition, it was found that, from 1979 to 2013, mortality rates fell, however, the numbers remain alarming, especially in the North and Northeast Regions. Thus, the importance of measures to prevent cervical cancer, such as vaccination and preventive examinations, as well as health education, is emphasized.

PROFILE OF ONCOLOGICAL PATIENTS SERVED IN A CHEMOTHERAPY OUTPATIENT CLINIC

CFSP167

THALES BRENDON CASTANO SILVA1; CASSIA APARECIDA DE OLIVEIRA2. Universidade Federal de Minas Gerais, Belo Horizonte - MG - Brazil; 2Hospital da PolĂcia Militar de Minas Gerais, Belo Horizonte - MG - Brazil. 1

Introduction and objective: Cancer is the second most common cause of death in Brazil, with an estimated 420,310 new cases in 2016. The most frequent types of aggressive cancers in Brazilians are breast and prostate cancer. Knowing the sociodemographic and clinical profile of patients assists in the support for actions aimed at responding to the global needs for palliative care and care. The objective of this study is to outline the profile of patients seen in 2017 in an oncology outpatient clinic. Materials and methods: Cancer is the second most common cause of death in Brazil, with an estimated 420,310 new cases in 2016. The most frequent types of aggressive cancers with in Brazilians are breast and prostate cancer. Knowing the sociodemographic and clinical profile of patients assists in the support for actions aimed at responding to the global needs for palliative care and care. The objective of this study is to outline the profile of patients seen in 2017 in an oncology outpatient clinic. Results and conclusion: A total of 275 patients were analyzed, the majority were women (67%), 47% were 60 to 79 years of age, 45.5% had breast cancer, 22% had prostate cancer and 2.5% had cancer of colon or uterus. Forty-four drugs were prescribed, gosserelin, tamoxifen and anastrozole were the most prescribed, corresponding to 17.4%; 15.2% and 13.8% of prescriptions. The profile of the patients is in line with the national profile, the prevalence of cancer types and the drugs according to recommended treatment protocols.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP168 PROFILE OF SELF-MEDICATION OF ELDERLY PEOPLE IN A PRIVATE HEALTHCARE INSTITUTION LILIANE DE OLIVEIRA DO NASCIMENTO; ROBERTA SOLDATELLI PAGNO PAIM. Centro Universitário da Serra Gaúcha, Caxias do Sul - RS - Brazil. Introduction and objective: The increasing elderly population and growth of chronic diseases demands a greater consumption of medication. Self-medication practices amongst elderly people and the excessive and concomitant use of different medication increases the risks of its usage, with a greater probability of intoxication and adverse collateral effects. This study aims to identify the occurrence of self-medication of elderly people in a private health-care institution. Materials and methods: Cross-sectional descriptive study with a quantitative approach, the data being collected through a questionnaire. The data was organized in tables and graphics, with the frequency in percentage and absolute numbers and analyzed through descriptive statistics. The research followed the guidelines of National Health Board and was approved by the Ethics Research Committee, certificate number 1.764.756. Results and conclusion: Self-medication among elderly women, with lower income and education levels was higher compared with elderly men with better income and education levels. Of all 43 elderly people interviewed, 74.42% reported using medication without medical prescription. Among the aspects for this usage are: easy purchase of medication in drugstores (50%), practicality and convenience (37.5%), lack of access to health-care services (15.63%) and lack of financial resources (9.38%), friends, neighbors and relatives being those responsible for the majority of suggestions without medical prescriptions. In five cases, there were health problems related to self-medication, being allergies the main adverse effect reported. Muscular pain (71.87%), headache (25%) and inflammation (18.75%) were the main reasons that led the interviewee to self-medicate. The most common medication used are painkillers and anti-inflammatory drugs. A higher prevalence of elderly people using neither prescribed nor oriented medication was perceived, exposing this patients to adverse effects and risks of toxicity. It is necessary to develop strategies and promote correct and rational usage of medication to this part of the population.

QUALITY OF MEDICAL PRESCRIPTION FOR PREGNANT WOMEN FROM LEGAL AMAZON

CFSP169

WEMBLEY RODRIGUES VILELA. Ufmt, Barra do Garças - MT - Brazil. Introduction and objective: Pregnancy is a delicate period for women, containing several hormonal and immunologic alterations to make fetal development possible, being medicines being able to promote fetal alterations. Drug prescription in gestational period should be very well elaborated, considering all possible effects for mother and fetus, evaluating their safety. The objective of this study is to evaluate medical prescription of medicines for pregnant women of Legal Amazon during prenatal period. Materials and methods: This is a pharmacoepidemiologic, descriptive, retrospective and cross-sectional study. Through medical records, sociodemographic variables, most prescribed pharmacologic groups, risk classification of medicines, inadequate use of medicines and possible drug interactions in pregnant women were evaluated. Results and conclusion: 159 medical records of pregnant women that use the United Health System (known as Brazilian SUS) were used. The most prescribed groups were antianemic preparations (52.9%), vitamins (12.5%) and analgesics (10.6%). According to the risk classification, the major prevalence of prescribed drugs belongs to A risk group (46.8%), followed by C group (28.9%), B group (20%), D group (4.3%) and none of X group. Among inappropriate drugs prescribed for pregnant women, the use of bromopride (30%) and magnesium hydroxide (20%) stand out. 8 possible drug interactions were found, 2 considered lowrisk and 6 considered moderate-risk. The results demonstrate that failures still exist in medical prescription of drugs for pregnant women and this can put the mother and fetus’s health at risk. Medical records are an essential effective therapeutic tool which should be read, analyzed and reviewed to guarantee effective and safe drug treatment Financial support or fellowship: CNPq; CAPES.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP170 QUANTITATIVE DESCRIPTIVE ANALYSIS OF MEDICAL PRESCRIPTIONS CONSONANT WITH RESOLUTION Nº586/2013 OF THE BRAZILIAN FEDERAL PHARMACY COUNCIL (CFF) AND PRACTICABLE BY PHARMACISTS REINALDO MEDINA OLIVEIRA; KARLA ROBERTA MESQUITA DA SILVA; DANIELA MARTINS DA SILVA. Universidade Paulista, Santos - SP - Brazil. Introduction and objective: Pharmaceutical prescription, regulated by resolution No. 586/2013 of the Brazilian Federal Pharmacy Council (Conselho Federal de Farmácia - CFF), is defined as the act in which the pharmacist selects and registers pharmacological and non-pharmacological therapies, as well as other interventions relating to patient health care. The pharmacist may perform prescription of medicine and other products for therapeutic purposes, which dispensation does not require medical prescription. This article aims to determine the occurrence of medical prescriptions feasible for pharmacists. Materials and methods: A survey was conducted of 1,200 medical prescriptions from health units with medicine dispensation: Hospital Pharmacy (Farmácia Hospitalar), Compounding Pharmacy and Drugstore, between September 1st, 2016 and September 10th, 2016. The pharmacist feasibility study was verified in accordance with the Brazilian Health Surveillance Agency (ANVISA website). Results and conclusion: 15% of the medical prescriptions were feasible for pharmacists and 85% were not. At Hospital Pharmacy 25% were feasible for pharmacists, 18% at the Compounding Pharmacy and 3% at the Drugstore. The pharmacological classes most represented in medical prescriptions feasible for pharmacists were Non-steroidal anti-inflammatory drugs 39%, Topical Antifungal 18%, Topical Antibacterial 17% and Antipyretic 10%.

RATIONAL USE OF MEDICINAL PLANTS

CFSP171

GABRIELE INÁCIO DE OLIVEIRA1; JOSE CARLOS DUARTE2; RENE OLIVEIRA DO COUTO1. Universidade Estadual de Londrina, Londrina - PR - Brazil; 2Universidade Estadual de Lodrina, Londrina - PR - Brazil.

Introduction and objective: The use of medicinal herbs and phytopharmaceuticals in the treatment of several illnesses is a common practice worldwide. For this reason, the phytotherapy comes up as a great field for Pharmacists to perform pharmaceutical care in order to achieve the efficacy and safety of this therapeutic strategy. Thus, the research group in Phytotherapy of the Health Sciences Center at the State University of Londrina aimed to give information concerning the rational use of such products to Londrina’s community by means of workshops and lectures. Materials and methods: The workshops and lectures were held at several strategic locations of the communities in some Health Basic Unities of Londrina city. A herbarion was organized with the most popular species of medicinal plants used by Londrina’s population. Their main characteristics as regards cultivation, collection, processing, formulation and medicinal use were systematically discussed. Results and conclusion: More than 60 participants including health community agents were included, who became amplifiers of the rational use of such kind of medicines. Furthermore, Professors and undergraduate students increased their knowledge in terms of public health and complementary medicine, as well as practiced their professional attributions and initiated a fruitful collaboration with communities. This emphasizes the importance of Pharmaceutical care, justifies the continuity purpose of our work and brings the possibility of further investigations involving the consumption of phytomedicines. Financial support or fellowship: FUNDAÇÃO ARAUCÁRIA; PROEX UEL

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP172 REFERENCE AND GENERIC MEDICINES: INFORMATION AS A FACTOR DETERMINING CONSUMPTION MARCO ANTONIO VALENTE1; JULIANO BET MARTINS2; ADALBERTO ALVES DE CASTRO2; LEONARDO DE PAULA MARTINS2; CLÁUDIO SÉRGIO DA COSTA2. Centro Universitário Barriga Verde - Unibave, Orleans - Brazil; 2Centro Universitário Barriga Verde - Unibave, Orleans - SC - Brazil. 1

Introduction and objective: : Every medicine is a pharmaceutical product, technically obtained or elaborated, with purpose of cure, healing, palliative or for diagnostic ends. They can be separated into three general classes: reference, similar and generic. The generic medicines, topic of this study, appeared to increase the competition among the producers of medicines and to reduce the cost of the treatments, treatments that can weigh heavily on the family budget, leading to a drop in family income for Brazilians. In this way, the main objective of the study is to investigate among clients of a pharmacy in a municipality in the south of the State of Santa Catarina, which information supports the choice, at the time of acquisition, between reference and generic drugs. Materials and methods: In this context, a research based on interviews with customers of a drugstore of small municipal district of the south area of Santa Catarina took place, with the purpose of collecting information on referred knowledge. Results and conclusion: The results confirm the need for a nation-wide campaign, with informative material that can give support to the patients to decide about the acquisition of a reference and/or generic product. Also, to increase the medical community’s understanding on the benefits of the use of generic medicines in the care of health, and to supply this information to their patients. CFSP173 REPORT OF EXPERIENCE OF THE ACTIVITIES OF A PHARMACEUTICAL RESIDENT IN ELDERLY HEALTH IN AN OBESITY GROUP IN BASIC CARE GILMAR DA SILVA ARAÚJO; CAMILA CRISTINA MARIN; ANDREA DE ANDRADE RUGGIERO; ANA ELISA PRADO CORADI. Fmabc, Santo André - SP - Brazil. Introduction and objective: In recent decades the Brazilian population, besides increasing the number of elderly people, has been showing a decline in nutritional parameters, promoting malnutrition and obesity. An aggravating factor directly related to the overweight or obese population is associated or acquired comorbidities: dyslipidemias, diabetes and cardiovascular diseases. Among the treatments are pharmacological and non-pharmacological therapies. With regard to pharmacological treatment, for a positive prognosis, it is necessary to adhere to the treatment. Objective: To present the experience of the pharmacist in an obesity group with elderly patients in basic care. Materials and methods: It is a report of the main experiences of the pharmacist in an obesity group already established by nutritionists. The pharmaceutical activities were developed during the first year of Health Multidisciplinary Residence Program of the elderly of the Faculdade de Medicina do ABC. Results and conclusion: The activities undertaken in the groups started with related issues: rational use of drugs; storage and disposal of medicines at home; diabetes; hypertension, use of anti-inflammatories and the relationship with renal insufficiency; multivitamins; medicinal plants; phytotherapeutic and homeopathic medicines; physiology of aging; drug interaction. The activities developed in the groups had as materials, handouts, blackboard, data show among other resources that enabled dynamic groups with the participation of the patients. The adherence to care was observed by the pharmacist through the report of the patients themselves as changes in habits and the improvement of the quality of life.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP174 RESEARCH OF ENTEROPARASITES IN SAND IN THE MUNICIPALITY OF ARIQUEMES, RONDONIA KEILEANE BEZERRA DA SILVA; ENEIAS MARCELINO DA ROCHA; FABIA MARIA PEREIRA DE SÁ ZEFERINO; SILVANA ALMEIDA DA SILVA. Faculdades Associadas de Ariquemes-Faar, Ariquemes - RO - Brazil. Introduction and objective: Basic sanitation, one of the means of disease prevention, is considered one of the most important activities carried out by public health, and most of the health problems affecting the world population are intrinsically related to the environment, more specifically to inadequate basic sanitation conditions. Some examples are parasitic diseases, often related to soil contamination, which depends on the destination of human waste and on the individual hygiene and sanitation conditions of the community, thus, the soil is responsible for contamination of various parasitic diseases, including the sands of playgrounds in squares, frequented by children of all ages. Thus, the objective of this work was to perform parasite research in the areas of playgrounds located in squares in the municipality of Ariquemes, Rondonia. Materials and methods: The research was carried out in three squares of the municipality of Ariquemes, Rondonia, and the sand samples were collected once a month in the period from August 2016 to January 2017, totalizing 54 samples. At the time of sampling, the samples were stored in sterile containers, labeled and stored, protected from light and heat until the analysis. For the parasite research, the methods of spontaneous sedimentation (Hoffman’s method) and the Willis method were used. Results and conclusion: After the analyses, it was observed that the sands of all the sites of collection had an index of contamination by parasites, being found Ascaris spp., Ancylostoma spp. and Entamoeba spp. In relation to the forms of contamination, at sites 1, 2 and 3 eggs of Ascaris spp. were found, at site 2 larvae of Ancylostoma spp. and at site 3 trophozoites of Entamoeba spp. Regarding the methodologies chosen for analysis, the Hofmann method detected the parasite forms, whereas, with the Willy method, there was no parasite detection. Thus, these data suggest the need for sanitation of these places frequented by children. CFSP175 RISKS OF DRUG INTERACTION OF THE HERBAL MEDICINE CASCARA BUCKTHORN - RHAMNUS PURSHIANA DC. RHAMNACEA WITH DIURETIC AND ANTIDIABETIC DRUGS DENISE LOPES MARUFFA MICHELIN. 50181, São Paulo - SP - Brasil. Introduction and objective: To orient healthcare professionals on the relevance of the risks of drug interaction of Cascara Buckthorn – Rhamnus purshiana DC. Rhamnacea with diuretic and antidiabetic drugs, which can damage the patient’s health during concomitant use, since Cascara Buckthorn has a laxative effect and can cause irritation in the intestines by promoting an increase in the peristaltic movements with subsequent loss of potassium and hypokalemia able to interfere in the secretion of insulin by beta cells, once the hormone controls plasma potassium through the interference in the activity of the Na+ K+3:2 ATpase pump. Cascara Buckthorn changes the intestinal microbiota and, associated with diuretic and antidiabetic drugs, can cause predisposition to relevant drug interactions with loss of efficacy and safety, putting the patient at risk. Materials and methods: Bibliographic review, by using literature and scientific articles, SciELO, articles from the last 5 years, PubMed, Medline, Lilacs and Science Direct on cascara buckthorn and interaction risks with diuretic and antidiabetic drugs. Results and conclusion: The risks of interaction of Cascara Buckthorn with antidiabetic and diuretic drugs is related to the change in the extracellular concentration of potassium and the change of microbiota thus affecting the absorption of vitamins and mineral salts that are important for homeostasis, which makes monitoring the associated parameters important in order to prevent risks.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CFSP176 SELF-MEDICATION PRACTICED BY STUDENTS OF PHARMACY AT UNIVERSITY OF CENTRO UNIVERSITÁRIO PADRE ANCHIETA JUNDIAÍ – S.P. SABRINA DE ALMEIDA MARQUES1; JESSICA CURVAL LEANDRO1; APARECIDA ERICA BIGHETTI RIBAS2; NATÁLIA CASTANHA DA SILVA1; VERONICA CRISTINA GOMES SOARES1. Centro Universitário Padre Anchieta, Jundiaí - SP - Brazil; 2Centro Universitário Padre Anchieta, Jundiaí - Brazil.

Introduction and objective: Self-medication is common and is growing in Brazil and worldwide. In university health care courses, this is more common due to the influence of their knowledge, which promotes the practice of irrational use of drugs, creating a public health problem. According to the World Health Organization (WHO), most medicines are sold or dispensed inappropriately and therefore people do not make correct and conscious use of them. As a result, this study assessed self-medication in 148 university students aged 18 years old and above on the course of Pharmacy of Centro Universitario Padre Anchieta. Materials and methods: The method used was a field survey, which contained a questionnaire on the various types of occasions when people used these medications without consulting a health professional and if they were aware of possible risks. This project was submitted to the ethics committee of Unianchieta following the Resolution CNS n. 466/12 and was approved. Results and conclusion: Of the total respondents, 82% are women. Out of 100% of respondents, 99% say they know the meaning of self-medication and its risks such as side effects, interactions of other medicines and others. It was noted that students with higher level of clarification regarding medication had a greater self-medication rate when it comes to painkillers, antipyretics, gastric protectors and even prescribed drugs such as antibiotics and psychotropic drugs, which is alarming, given that these medicines are controlled by 344/98 Resolution. Not all classes of drugs were associated with self-medication, but it was observed that knowledge influences the increasing frequency of self-medication of students according to the semester they are in. This study shows that many undergraduate students use medicaments without consulting a physician and this increases as they study more about medications but this behavior must not be encouraged. It should be emphasized that future pharmacists must be ethical and they should be aware of their importance in teaching others the rational use of medicines. CFSP177 SENSITIVITY AND SPECIFICITY OF FREE ONLINE DATABASES FOR DRUG INTERACTIONS IN DIABETIC PATIENTS SANDRO RITZ ALVES BEZERRA. Universidade de São Paulo/Secretaria de Estado da Saúde, São Paulo - SP - Brazil. Introduction and objective: The pharmacotherapeutic diagnosis of Drug Interactions may vary in the different databases available online. The Objectives of this work is to compare the sensitivity and specificity of the databases for DDIs of Medscape and Drugs.com in relation to Micromedex. Materials and methods: From the potential drug interactions detected in pairs with the use of Micromedex in the analysis of medical prescriptions of specialized medical outpatient clinics for diabetic patients, the interactions of the same drug pairs were searched for in the free online sources: Medscape and Drugs.com. Results and conclusion: Statistical analysis revealed only a fair correlation of Drugs.com and a non-correlation of Medscape with the kappa correlation coefficient. Simultaneous use of the two databases generates a sensitivity of 95.10% in relation to Micromedex. In this study, the sensitivity for the detection of drug interactions was of 75.49% for Medscape in relation to Micromedex and 90.20% for drugs.com in relation to Micromedex. The specificity of Medscape was 56.80% and the specificity of drugs.com was 62.73%. In Conclusion, the use of the two databases generates a sensitivity of 95.10% and specificity of 75.50%, in relation to Micromedex. In the contraindicated interactions both databases had performance equal to Micromedex.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

SIGNALING PATHWAY OF NF-KB IN RAT PLACENTAS IS MODULATED BY HYERGLICEMIA

CFSP178

ÍTALO RICARDO TAVARES FRANCO. Ufmt, Barra do Garças - MT - Brazil. Introduction and objective: The placenta is essential in fetal growth and support during pregnancy. Its function becomes compromised in several situations, such as hyperglycemic gestation, resulting in restriction of fetal growth, or even leading to death. In hyperglycemic pregnancies, the expression of some inflammatory mediators is increased, causing dysregulation of placental function. Under hyperglycemic conditions, activation of the nuclear transcription factor NF-κB occurs, which is present in most cells as a dimer, consisting of two subunits: p65 (RelA) and p50. This dimer is located in the cytoplasm attached to its inhibitor IkB. Objective: Evaluating the effect of hyperglycemia on protein expression in the NF-κB signaling pathway in rat placentas. Materials and methods: 12 to 14-week-old Wistar rats from 200 to 250g were randomly divided into the hyperglycemic group (streptozotocin (40 mg/kg, ip; glycemia>400 mg/dL) n=7] or normoglycemic (vehicle; n=6, glycemia<110 mg/dL), and submitted to mating. We extracted and quantified the proteins p65, total IkB and phospho-IkBSer536 by the Western Blot technique. The results were expressed as mean±standard error of the mean (SEM) of each group. Statistical significance was determined using the Student’s t-test, and the P-value calculated by the “t” ratio and the degrees of freedom using the Prism program (Graph Pad Software Incorporated, CA). Values of P><0.05 were considered statistically significant. All procedures were approved by the animal ethics committee (UFMT, 23108.120946/2015-83). Results and conclusion: The p65 subunit of the transcription factor NF-κB showed no alteration in the placental tissues analyzed. The expression of IκB, the transcription factor inhibitor NF-κB, showed a significant decrease in the hyperglycemic group compared with the control group. Expression of its phosphorylate form, IκB-phosphorylated (Ser32) showed a significant increase when compared to the control rats. The ratio of the phosphorylated and total form of the IκB protein was increased in the hyperglycemic group, characterizing a greater activation of this protein, favoring the translocation of the NF-κB transcription factor to the nucleus. Conclusion: The results suggest that hyperglycemia leads to increased activation of the transcription factor. Financial support or fellowship: Financial support: FAPEMAT; CNPq. CFSP179 SITUATIONAL DIAGNOSIS OF PHARMACEUTICAL CARE SERVICES IN BASIC HEALTH UNITS IN THE CITY OF SÃO PAULO MARIA GABRIELA BORRACHA1; SILVIA STORPIRTIS2. FCF-USP, São Paulo - SP - Brazil; 2FCF-USP, São Paulo - Brazil.

Introduction and objective: Objective: To elaborate a situational diagnosis of the pharmaceutical care services carried out at Primary health care centers (UBS) in the city of São Paulo. Materials and methods: The situational diagnosis was performed by analyzing the answers of 38 pharmacists who work at the UBS in the city of Sao Paulo using an online questionnaire. Results and conclusion: The majority (92%) of the pharmacists interviewed are women, with an average age of 37 years old, and an average of 11 years of training and 5 years working in health units; 76% have some type of post graduation; 29% work in more than one health center and 91% work 40 hours a week. The pharmacy staff is usually formed of 3 employees, and they check an average of 430 prescriptions daily; 84% of the units in which pharmacists work are administered by OSS and 66% have ESF teams; 82% of the units have room for consultation; 68% of pharmacists perform some clinical activity, to which they devote 15% of their workload; 50% of pharmacists conduct consultations (15 consultations/month) and 53% of pharmacists do home visits (HV), averaging 12 HV/month. Only 21% have a standard form to conduct this service. The main criteria for patient selection is the referral of patients by a professional of the unit. The physicians are the professionals that most refers patients to pharmaceutical consultations. Conclusions: In order to conduct the clinical activities of pharmacists within the health units, investments are still required for hiring and training pharmacists, as well as standardization of models for realization and evaluation of these services, which would help the implementation of these services and their quality.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

SLEEP QUALITY AMONG UNIVERSITY STUDENTS FROM JUNDIAÍ - SP

CFSP180

MARIVANIA ALVES PEREIRA1; ROSANGELA BARROS DA SILVA1; VERONICA CRISTINA GOMES SOARES1; LEANDRO DOS SANTOS2; HEDER FRANK GIANOTTO ESTRELA1. Centro Universitário Padre Anchieta, Unianchieta, Jundiaí - SP - Brazil; 2Universidade Federal Rural de Pernambuco – Uast/ Ufrpe, Serra Talhada - PE - Brazil. 1

Introduction and objective: The technology required of man a great adaptation to accompany the dynamism and increase of productivity, sacrificing hours of sleep in search of qualification for the labor market, there is the possibility of disturbances in sleep in people who study, work and assume other responsibilities. So, irregular sleep patterns can cause a variety of harm, such as cognitive failure, metabolic and endocrine changes, early aging, and increased morbidity. The aim of the present study was to evaluate sleep quality in university students from Jundiaí-SP. Materials and methods: Sleep quality was measured using the Pittsburgh Sleep Quality Index (PSQI). The study group comprised 174 university students from Padre Anchieta University, Jundiaí-SP. Data were collected between September and October, 2015. Ethics approval for the study was granted by the Ethics Committee of the UNIANCHIETA (certificate 1.214.729).” Results and conclusion: The random sample comprised 174 subjects, containing both women (n=144) and men (n=30). The PSQI global scores for the participants were 7.7 ± 1,1. According to the PSQI, global scores above 5.0 show impairments in sleep quality. Subjective sleep quality was 1.5 ± 0.4; sleep latency was 1.3 ± 0.2; sleep duration was 1.62 ± 0.3; habitual sleep efficiency was 0.22 ± 0.08, and use of sleeping medication 0.3 ± 0.02. The findings from this study revealed that there were higher numbers of poor sleepers, evaluated by the global score above 5. In each component evaluated, a score that showed poor sleep quality was observed. It was determined that few subjects used sleeping pills. CFSP181 SOCIOECONOMIC PROFILE OF HIV CARRIERS PARTICIPANTS IN AN NGO LOCATED IN THE CITY OF CAMPINA GRANDE-PB WILLIAN CHARLES DA SILVA MOURA1; ÍTALA SAMARA DA SILVA DIAS1; ZILKA NANES LIMA1; GERALDO EDUARDO GUEDES DE BRITO2; RICARDO OLIMPIO DE MOURA1. Universidade Estadual da Paraíba, Campina Grande - PB - Brazil; 2Universidade Federal da Paraíba, João Pessoa - PB - Brazil.

Introduction and objective: The wide dissemination and rapid advancement of the Human Immunodeficiency Virus (HIV) demonstrate that this epidemic affects different age groups and socioeconomic levels. This study aimed to discover the socioeconomic profile of HIV/AIDS patients attending the Life Support Group (GAV), an NGO located in the city of Campina Grande, PB. Materials and methods: A cross-sectional and analytical study was carried out involving a sample of 21 participants of both sexes, who underwent an interview to fill out a questionnaire containing questions about socioeconomic profile including schooling, monthly family income, and antiretroviral treatment time. Data were analyzed descriptively. Results and conclusion: The study population had a homogeneous distribution among the genders, 52.4% being female, concentrated in the age group between 30 and 59 years (66.7%) and the majority with a diagnosis of 10 years (81%). It was verified that 86.7% had a family income between 1 and 2 minimum wages. The level of schooling was 57% with incomplete elementary education, 33.4% between elementary and high school, and only 9.6% had incomplete higher education. The results show that the socioeconomic profile of the studied population did not differ from other studies in similar situations, even taking into account the regional differences and the various faces of the epidemic in Brazil. Financial support or fellowship: PROAPEX; UEPB.

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CFSP182 STRATEGIC PLANNING IN THE DISPENSATION OF MEDICINES IN THE OUTPATIENT PHARMACY OF A STATE PUBLIC HOSPITAL LUIS HENRIQUE OLIVEIRA REZENDE1; ALEXANDRE MANSUÊ FERREIRA CARNEIRO2; MARCELA CRISTINA DE PAULA3. Iamspe, Sp - SP - Brazil; 2Instituto de Assistência Ao Servidor Público Estadual, Sp - SP - Brazil; 3Instituto de Assistência Ao Servidor Público Estadual, Sp - SP - Brazil. 1

Introduction and objective: The outpatient pharmacy requires a complex and efficient structure of uptake, orientation and care, ensuring the best form of adherence to treatment, without causing discomfort or inefficient care to its patients. The objective of this work was to implement a pharmaceutical management plan, aiming at reducing the waiting time in the withdrawal of medicines. Materials and methods: For this purpose, Situational Strategic Planning (PSS), based on problems and operations that need to be defined and carried out in order to deal with these same problems, is applied. Therefore, an operational plan (OP) was developed, based on four stages: explanatory moment, normative, strategic and tactical-operational. Results and conclusion: Where the average daily waiting time (AWT) for drug withdrawal was approximately 2h30min50s. After the application of the PO, a reduction of AWT of 1h30min05s was observed. Measures adopted such as: review of routines established for attendance, replanning of the physical space, number of service counters, acquisition of a more efficient computerized hospital management system and implementation of the Home Health program, where patients with chronic diseases receive their medicines at home, strategies were adopted that corroborated this reduction of AWT. This study led to the dynamization of the dispensing of medicines, as well as a better understanding of the routines established in this service within the hospital. CFSP183 STRATEGY FOR CHOOSING FREE ONLINE DATABASES FOR DRUG INTERACTIONS IN DIABETIC OUTPATIENTS: SENSITIVITY AND SPECIFICITY IN RELATION TO MICROMEDEX SANDRO RITZ ALVES BEZERRA. Universidade de São Paulo/Secretaria de Estado da Saúde, São Paulo - SP - Brazil. Introduction and objective: The objective of this work is to verify the best strategy for choosing free databases available online in relation to Micromedex in the research of Potential Drug-drug Interactions (DDI) in diabetic outpatients. Materials and methods: Cross-sectional study. From the potential drug interactions detected in pairs with the use of Micromedex, the interactions of the same pairs of medicines were verified by using ten free online databases. We analyzed two types of strategy for choosing database: 1) Using absolute and relative frequency data. 2) Using the Kappa correlation coefficient of the data. Results and conclusion: Using strategy 1, Meryland’s database presented the best index of specificity in relation to Micromedex. The second one was UK HEALTH CARE. The use of both databases approached the results obtained by the Micromedex base in 98.04% sensitivity and 74.50% specificity. Using strategy 2, Meryland’s presented the best result, followed by Drugs.com and the use of both databases approached the results obtained by Micromedex in 99.02% of sensitivity and 75.50% of specificity. In conclusion, the two databases chosen from the Kappa’ coefficient analyses were slightly higher.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

STUDY FOR THE USE OF ANTINEOPLASTICS IN A HOSPITAL IN 2016

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CFSP184

THALES BRENDON CASTANO SILVA1; CASSIA APARECIDA DE OLIVEIRA2. Universidade Federal de Minas Gerais, Belo Horizonte - MG - Brazil; 2Hospital da Polícia Militar de Minas Gerais, Belo Horizonte - MG - Brazil. 1

Introduction and objective: Cancer is one of the most prevalent diseases in the world, a frequent cause of personal suffering, and a concern for health systems for expensive, complex and chronic treatment. Studies of drug use in pharmaceutical care help enable the rational use of health resources, especially in the planning and procurement of medicines. The objective of the study was to describe the most used antineoplastic drugs in the hospital in 2016. Materials and methods: Descriptive study of the use of antineoplastic drugs prescribed in an oncology outpatient clinic. The data of all prescriptions issued in 2016 were collected in an instrument (excel sheet) previously prepared and validated for this purpose. The following variables were searched: number of prescriptions, number of formulations, intravenous and oral chemotherapy regimen. The data were included through the data collection instrument developed for this purpose. Results and conclusion: There were 3,156 prescriptions during the year, with an average of 263 per month (231-292), totaling 9,649 formulations. The most commonly prescribed intravenous antineoplastic agents were Fluorouracil 250 mg (2,515 flasks), Folinic Acid 50 mg (2,265 flasks), Paclitaxel 30 mg (406 flasks) and Gemcintabine 200 mg (405 flasks). Of oral use, the most prescribed were Tamoxifen 20 mg (1,1204 tablets), Anastrozole 1 mg (9,603 tablets), Capecitabine 500mg (5,872 tablets) and Bicalutamide 50 mg (2,298 tablets). Knowing the profile of the use of antineoplastic drugs from an oncology unit is important to establish priorities and quantities to be purchased, as well as avoid unnecessary acquisitions, losses and breaks in supplies of the availability of resources. CFSP185 STUDY OF THE PACKAGE INSERTS IN CONSUMER INSIGHT VIEW AND ITS ROLE IN PROMOTING THE RATIONAL USE OF MEDICINES LUISI ARAUJO FILIPINI; JOSELAINE DE OLIVEIRA; MARIANE MACHADO CURBETE. University Paulista - UNIP, Araçatuba - SP - Brazil. Introduction and objective: The package insert accompanying the medication is considered to be the main source of information for patients, because it clarifies doubts about the treatment of the diseases and is as important as the information provided by the doctor in the consultation and described in the prescription. The form and content expressed in the package leaflet are legally determined by ANVISA and must follow the evolution of the standardization of the pharmaceutical sector. Objective: to evaluate the use of the package leaflet as an information tool for the consumer and to study their role in promoting the rational use of medicines. Materials and methods: Descriptive cross-sectional field study, of observational character, prospective, qualitative and quantitative conducted at a drugstore in the city of Araçatuba, Sao Paulo state using a questionnaire applied to 50 clients over 18 years old. Results and conclusion: The majority of people interviewed were women (62%) with 56 years of age or older (64%), with a high school education (48%), who use 1 to 3 medications daily (52%) and who have the habit of reading the package insert (58%), especially the indications item (66%). In contrast, difficulty was noted in relation to the reading due to the font size (30%) and the negative influence of the item adverse effects (43%). Among the interviewees, 52% believe that the adverse reactions cause fear of taking the medicine, 66% use the package leaflet to clarify doubts and 64% say that they are healed after reading. In addition to the fact that the package leaflet is considered indispensable (80%), the pharmacist is also sought to clarify doubts regarding the use of medicines (72%), which reflects the importance of pharmaceutical care for patient adherence to pharmacotherapy and the contribution to the rational use of medicines.

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CFSP186 STUDY OF THE PREVALENCE OF SELF-MEDICATION AMONG STUDENTS OF THE HEALTH SCIENCES CENTER AT A PRIVATE UNIVERSITY OF FORTALEZA CE. MARIA CLARA CAVALCANTE DE LACERDA; FRANCISCO WENDELL XAVIER BARBOSA; RENAN PEREIRA DE LIMA; MARIA ANGELINA DA SILVA MEDEIROS; VICENTE BARROS DE LIMA NETO. Unifor, Fortaleza - CE - Brazil. Introduction and objective: Self-medication is a global phenomenon and is therefore a public health problem. It is a common form of health care, consisting of the consumption of a product with the objective of treating or alleviating symptoms or perceived diseases or promoting health, regardless of professional prescription, which may have uncertain results and great health risks. This study aimed to determine the prevalence of self-medication among students describing the reasons that influenced the predisposition to self-medication and the classes of medications used for the practice of self-medication. Materials and methods: Sectional and descriptive study, conducted between February and March 2017. A data collection instrument was applied and the data were evaluated in Epi Info software in version 3.5.1. The project was approved by the ethics committee, certificate number 675,686. Results and conclusion: We evaluated 179 students from the 1st to the 10th semester of the Pharmacy degree course, with an average age of 21.72 ± 0.24 (x ± SEM), where a predominance of female students 75% was observed (CI95% 68.4 – 81.5) and the highest prevalence was of students enrolled in the 5th semester with a percentage of 18.45% (CI95% 13.0 -24.9). A total of 97.2% (CI95% 93.6 - 99.1) self-medicated, especially with over-the-counter drugs such as non-steroidal anti-inflammatory drugs (NSAIDs) 192 (37.4%) and analgesics (64.5%). The symptoms that led to the practice of self-medication include menstrual cramps, headache, diarrhea, stomach ache, flu and allergic processes. The experience they already had with the medicine and having the medicine at home by medical indication were one of the main reasons for self-medication. Of the students who self-medicate, 46 (26%) (CI95% 19.7 -33.1) used old prescriptions as reference and the media influenced 50 (28.1%) (CI95% 21.6 - 35.3) for the Practice of self-medication. Therefore, there is a clear need to disseminate the risks of self-medication and to address educational measures to promote the rational use of medicines. Financial support or fellowship: TUTORIAL EDUCATION PROGRAM (PET). CFSP187 STUDY ON THE PERIOD OF USE OF MULTIPLE DOSE MEDICINES AFTER OPENING THE PRIMARY PACKAGING THAYS DA SILVA ALMEIDA; ANA CAROLYNA VARGAS SERAPHIM. Hospital Naval Marcílio Dias, Rio de Janeiro - RJ - Brazil. Introduction and objective: Multidose drugs are those that allow the withdrawal of successive portions of their contents, without modifying the concentration, purity and sterility of the remaining portion. These drugs are among the most affected by storage and inadequate use. Several factors may influence their stability and may cause potential risks to patients. Considering that it is the hospital pharmacy’s role to ensure the safe and rational use of prescribed drugs, the objective of this work was to produce a guide containing information about the period of use and conservation of all standardized multidose drugs in the hospital of the study Materials and methods: To elaborate the present work bibliographic research was carried out from the descriptors in Portuguese, and their congeners in Spanish and English: stability, multidose and validity after opening the package in electronic databases (Medline, Scielo, Pubmed and Lilacs). For the preparation of the guide, the information contained in the drug leaflets was consulted and the rest of information was obtained from the manufacturers’ laboratories. Results and conclusion: We analyzed all the 91 standardized medicines in multidose packs from the hospital. Of the total number of drugs, 35 are administered orally, 20 by topical route, 17 by ophthalmic route, 11 by injectable route, 7 by nasal/pulmonary route and 1 by otological route. Approximately 46% of the information obtained on stability was collected through the drug leaflets and 54% was obtained through contact by e-mail or telephone with the manufacturer laboratories, and these were confirmed with data available in the literature. A table containing each drug, pharmaceutical form, the period of use of the drug after opening and the ideal storage condition was then elaborated. For medicines whose manufacturer’s laboratories did not present the information, the determination of the new validity was made taking into consideration the information contained in the RCD 67/2007 and other international resolutions. It is expected that with the development of this work, hospital health professionals will have easy, quick, effective and specific information about each drug, and this will be very useful to ensure optimization of patient therapeutics and the existing resources.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

SURVEY AND ANALYSIS OF PUBLICATIONS ADDRESSING PHARMACEUTICAL CARE

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CFSP188

JANAINA PENHA DE ALMEIDA PEREIRA. Centro Universitario das Faculdades Associadas de Ensino Fae, Caconde - SP - Brazil. Introduction and objective: Pharmaceutical care is defined as a model practiced by pharmacists that encompasses attitudes, ethical values, behaviors, skills, commitment and co-responsibilities in the prevention of diseases as well as the promotion and recovery of health in conjunction with the multidisciplinary team. The aim of the present study was to analyze scientific publications addressing pharmaceutical care. Materials and methods: Fifty national and international articles were analyzed with regard to the methods employed by the researchers, populations studied, proposed interventions and outcomes. Results and conclusion: The authors of the scientific articles used pharmaceutical care to ensure the rational use of medicines and diminish the risk of self-medication. Articles addressing pharmaceutical care have been published in the last ten years, with the occurrence of a significant increase in publications in 2008, but a reduction in the number of articles published in subsequent years. Analysis of the articles demonstrates that pharmaceutical care is a practice with positive aspects that is capable of producing benefits for both the patient and pharmacist, who is always ready to clarify questions related to medications. CFSP189 SURVEY OF THE USE OF DRUGS OF PATIENTS TREATED IN CAPS AD IN THE CITY OF CAJAZEIRAS IN THE PERIOD OF JANUARY TO JULY 2014 ANNALU MOREIRA AGUIAR1; KELLY SOUZA LIMA2; ANNA CLAUDIA MOREIRA AGUIAR2; RENATA ALMEIDA DE SOUZA ARANHA E SILVA1. Faculdade de Medicina do Abc Paulista, Santo AndrĂŠ - SP - Brazil; 2Faculdade Santa Maria, Cajazeiras - PB - Brazil.

Introduction and objective: The consumption of chemicals has only increased in recent times, use has become increasingly earlier by adolescents and the media have a very large influence over this consumption, so indiscriminate use is considered a public health problem. The CAPS AD (Psychosocial Attention Center in Alcohol and Drugs) is responsible for taking care of patients of all ages with psychological disorders and who use substances such as alcohol, tobacco, marijuana and crack. Thus, the aim of this research was to study drug use in patients at CAPS AD (PAC AD) in the city of Cajazeiras from January to July of 2014. Materials and methods: It is a descriptive-retroactive study that investigated the variables of gender, age, marital status, schooling, demand and types of psychoactive substances in use. In the analysis of the data Microsoft Office Excel 2007 was used, with the determination of the averages and the standard deviation of the results. Results and conclusion: The sample consisted of 107 users received at CAPS AD the city, with 77% male and 23% female, it was observed that the highest incidence of users in relation to age is 19-30 years old (37 %), unmarried (39%), the degree of schooling is incomplete primary education (33%), increased demand for the service occurs spontaneously (56%), and the most widely used drug is tobacco (23 %) followed by crack and other drugs (17%) and 16% alcohol. According to research carried out by Silva et al. (2006), Pillon et al. (2010) and Rocha (2014), the substances most used by users were alcohol followed by tobacco and other drugs of abuse. These data differ from the profile of users of the CAPS AD of Cajazeiras who use more tobacco followed by alcohol, crack and marijuana.

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CFSP190 THE ASSESSMENT OF ORGANOLEPTIC PHYSICAL CHEMISTRY PROPERTIES IN DRUGS REDISTRIBUTED BY THE UNIBAVE MEDICINE RECYCLING EXTENSION PROGRAM ALEXANDRE JOÃO MARTINS NETO1; PRISCILA MARIA AMORISO2; ANDRESSA CORNEO GAZOLA3; CARLOS HENRIQUE BLUM DA SILVA1; CLÁUDIO SÉRGIO DA COSTA1; ADALBERTO ALVES DE CASTRO3. Centro Universitário Barriga Verde-Unibave, Orleans - SC - Brazil; 2Centro Universitário Barriga Verde-Unibave, Urussanga - SC - Brazil; 3Centro Universitário Barriga Verde-Unibave, Criciúma - SC - Brazil. 1

Introduction and objective: This article`s general aim is to evaluate the organoleptic properties and the pharmacological efficiency of drugs donated to the UNIBAVE medicine recycling extension program. Materials and methods: The research was developed in the Biological Sciences and Health laboratory located at UNIBAVE university, in the city of Orleans. The analytical techniques were based on the guidelines laid out in the 5th edition of the Brazilian Pharmacopoeia, taking into consideration the fact that many drugs are not stored properly, generating the possible loss of their pharmacological activity. For the analysis, three drug samples were used: acetylsalicylic acid, nitrofurantoin and furosemide. The laboratory procedures performed to collect the data were based on the analysis of the visual aspect, the average weight calculation and thin layer chromatography. The practices were carried out simultaneously with samples of three drugs from a dispensing pharmacy compared to those from the donation program. Results and conclusion: Based on the results obtained in the research on the drugs in question, it can be concluded that all drugs samples were within the parameters specified in the current literature, that is, they did not present nonconformities that could significantly minimize their pharmacological effects. The development of the analytical techniques developed by the study ensure the pharmacological effectiveness of the presentations offered by the Medicines Recycling Extension Program of UNIBAVE, promoting the practice of rational drug use and significant savings to the city in addition to expanding therapeutic access to the population.

CAPECITABINE COST-BENEFIT RATIO ON COLON-RECTAL CANCER

CFSP191

TATIANE MARIA DO MONTE NOBRE; FRANCISCO WENDELL XAVIER BARBOSA; RENAN PEREIRA DE LIMA; ARLANDIA CRISTINA LIMA NOBRE DE MORAES. Unifor, Fortaleza - CE - Brazil. Introduction and objective: Capecitabine is indicated as both first line monotherapy to treat advanced colon cancer and as an adjuvant of stage III colon cancer. This makes it a part of the backbone of colon-rectal cancer treatment. Over the last few years, however, the use of capecitabine, a drug similar to 5-FU, has been disseminated. It acts primarily on the tumor tissue where it is converted to 5-FU under the effect of the thymidine phosphorylase enzyme. It is applied as oral tablets; they are prescribed as a 1250mg/m² body surface dose for fourteen days out of every twenty-one days for eight months total. This study had the objective of evaluating capecitabine cost-benefit ratio for colon-rectal cancer. It was conducted at an oncology center in Fortaleza-CE. Materials and methods: The analysis was performed from the comparison of its analog, fluorouracil (5-FU) in combination with leucovorin through a retrospective, observational and transversal study. In the first stage of the study, medical prescriptions were analyzed; of a total of 99 patients, 10 were selected based on the pre-defined eligibility criteria. Following that, they were grouped based on the treatment, which they received in the year 2009: capecitabine (Group A) and 5-FU/leucovorin (Group B). Subsequently, an analysis of both the cost-benefit ratio and of the benefits of both drugs was performed through an analysis of patient’ records over a period of six months. The study was approved by the Ethical Committee of Human Research from the Fortaleza University, certificate number 10-032. Results and conclusion: Regarding costs, it became evident that group B’s treatment was the most expensive. This was because their treatment was through leucovorin, the main reason for the increase of this parameter. Despite the lack of data, capecitabine’s benefits were related to fewer side effects, being then an effective and more economically viable alternative to colon-rectal cancer treatment.

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CFSP192 THE CHALLENGES AND INSPECTION OF THE PHARMACEUTICAL CLINIC IN THE FAMILY HEALTH PROGRAM TEAM AMANDA SALES CUSTODIO1; AMANDA CAVALCANTE VAZ DE MORAES2; ANDREA DE ANDRADE RUGGIERO2; ANA ELISA PRADO CORADI2; JULIANA LOPES CUSTODIO2. Fmabc, Santo André - Brazil; 2Fmabc, Santo André - SP - Brazil.

Introduction and objective: Introduction and objectives: The pharmaceutical clinic is an emerging area in expansion in Brazil. There are resolutions that protect the performance of the pharmacist. In the SUS, this professional is included in the family health program team, however, he does not always take a part of that nucleus. Objectives: to report the challenges found in clinical practice in family health program team. Materials and methods: The study is based on experiences of activities developed by pharmacists of the Multiprofessional Residency Program in Health of the Elderly and Cancer Care, by the Faculty of Medicine of ABC, distributed in the cities of: Santo André, São Caetano do Sul and São Bernardo do Campo in the period from March 2017 to June 2017. Results and conclusion: The pharmacist in the SUS is seen as a professional who dispenses drugs, and carries out administrative duties, which sometimes prevent him from performing clinical practice. There are professionals, and even the general population, who are unaware of the possible actions of the pharmacist in individual and community health care. It is necessary to make sure that the pharmaceutical profession occupies this space in family health teams. Financial support or fellowship: ABC Medical School - FMABC. CFSP193 THE CLINICAL PHARMACIST’S PARTICIPATION IN INTENSIVE CARE UNITS EMPHASIZING THE NEPHROTOXIC POTENTIAL OF VANCOMYCIN DANIELE VECCHIA DIONATO. Santa Marcelina, São Paulo - SP - Brazil. Introduction and objective: The Clinical Pharmacist’s participation contributes through activities that maximize patient security. Monitor and evaluate the nephrotoxicity occurrence caused by the vancomycin in hospitalized patients in adult ICU, by the clinical pharmacist engagement, to suggest the individual adjustment of the therapy, providing comfort and reducing risks. Materials and methods: The patients included in the research were followed during the antibiotic therapy, by monitoring of laboratory examinations, adjustments, suggestions of the doses according to vancomycin plasma levels and validation of medical prescriptions. Thirty-seven patients were included with the average age of 70 years. Results and conclusion: Among the studied patients, 97.3% of pharmaceutical interventions were able to be done. Acute Kidney Injury (AKI) characterized by the Acute Kidney Injury Network (AKIN) standard was observed in 16.2% of patients that only used vancomycin and in 54.1% of patients who used other nephrotoxic medicines associated with vancomycin. AKI frequency characterized by AKIN indicates that vancomycin associated with other nephrotoxic medicines shows a significant nephrotoxicity. So it is relevant to dose the serum levels of the antibiotic with the clinical pharmacist’s participation, which is a differential to succeed in the medicinal therapy.

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CFSP194 THE EPIDEMIOLOGICAL SITUATION OF SCHISTOSOMIASIS MANSONI IN THE SOUTHEASTERN REGION OF BRAZIL PEDRO HENRIQUE FERREIRA; JOSÉ TARCÍSIO FRANCO DE CAMARGO; ELIANA ANUNCIATO FRANCO DE CAMARGO. UNIPinhal, Espírito Santo do Pinhal - SP - Brazil. Introduction and objective: Schistosomiasis is still a public health problem, and is considered an important parasitic disease worldwide. In Latin America, Brazil is the country with the largest number of notified cases, totaling 98% of the region’s cases. Historically, the Southeastern region of the country is not recognized as an epidemic region. Because of this, few campaigns in this region are aimed at preventing transmission of schistosomiasis. This work was conducted aiming to report the epidemiological situation of the disease in the Southeast region. Materials and methods: A descriptive study, of a quantitative nature, was conducted on the epidemiological characterization of schistosomiasis mansoni in the Southeastern region of Brazil in the period from 2006 to 2015, which data refers to the notified cases in the System of Notifiable Aggravations (SINAN) of the Ministry of Health. Results and conclusion: The results indicated that, when the years of 2006 to 2015 are compared, the number of notifications in the region has decreased. However, the analysis that considers the percentage corresponding to all cases in the country points to a disturbing situation for the region. Until the year 2007, the region notified approximately 20% of all cases of schistosomiasis in Brazil—but in the subsequent years, this situation was reversed, reaching 93.2% of all Brazilian cases in the year 2010. In 2015, the percentage decreased to 76.2%. This data shows that the region needs attention concerning the issue with schistosomiasis—the population needs clarification and programs for the prevention of the disease need to be developed. Financial support or fellowship: Centro Regional Universitário de Espírito de Pinhal-UniPinhal.

THE EVALUATION OF ULCERATIVE RECTOCOLITIS DRUG TREATMENT

CFSP195

TATIANE MARIA DO MONTE NOBRE; ALAN RODRIGUES DA SILVA; RENAN PEREIRA DE LIMA; ARLANDIA CRISTINA LIMA NOBRE DE MORAES. Universidade de Fortaleza - Unifor, Fortaleza - CE - Brazil. Introduction and objective: Ulcerative rectocolitis (URC) is one of the most common inflammatory bowel diseases (IBDs). It is characterized by a chronic inflammation of the intestine. The etiology has not been definitely clarified yet. Its etiology remains poorly defined, it is suggested that it stems from cellular immunological abnormalities, namely the abnormal reactivity of the T lymphocytes from the gastrointestinal mucosa to a non-pathogenous normal microflora. However, the pathogenesis remains unknown. This study had the goal of identifying the main characteristics of the drug treatment prescription to the patients with ulcerative rectocolitis at a Medical Specialties Center in Fortaleza-CE. Materials and methods: This is a retrospective and qualitative study. A form was used as a tool to collect data from the medical records in which the drug treatment characteristics had been identified and evaluated. This was done for 30 medical records of patients with ulcerative rectocolitis (URC). The study was approved by the Ethical Committee of Human Research from the Fortaleza University, certificate number 027-2010. Results and conclusion: The data shows that all the patients took a URC confirmatory examination, predominating male patients from twenty to forty years old. It was possible to observe that the use of association of drugs can promote adverse reactions and interactions. Of the thirty medical records which were analyzed, eighteen showed associations of other drugs to sulfalazine and seven did not show any association with this drug. This study did not find any register of diverse reactions in the patients’ medical records. Therefore, the conventional therapy is adequate, it remains the only one available, justifying its standardization despite the cost.

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CFSP196 THE IMPORTANCE OF CLINICAL PHARMACY INSTALLATION IN A HIGH COMPLEXITY HOSPITAL DANIELE VECCHIA DIONATO. Santa Marcelina, São Paulo - SP - Brazil. Introduction and objective: Clinical Pharmacy is classified as a Health Science the responsibility of which is to ensure the rational usage of medicines by the application of knowledge.. Monitor and evaluate the pharmaceutical intervention indices held in the clinical emergency room for adults and intensive care units to emphasize the importance of prescription validation by the clinical pharmacist. Materials and methods: Classified as prospective study of exploratory basis, quantitative, carried out using field research, aiming to evaluate medical prescriptions and executing pharmaceutical interventions when necessary. All the analyses were performed using the statistic software SPSS (Statistical Package for the Social Sciences), version 23.0. Results and conclusion: The executed study showed that the service is well regarded by the healthcare staff, with high acceptability rate. The clinical pharmacy’s implantation in a high complexity hospital is an assertive advance, by that, is possible to boost patient safety through rational usage of medicines, the clinical pharmacist being very important and necessary in all sections of a hospital, a professional who fosters safety, minimizing the rate of avoiding reactions, reducing the time hospitalization, mortality rate and covering the hospital economy too.

THE IMPORTANCE OF PHARMACOTHERAPEUTIC FOLLOW-UP TO USERS OF MOROSIL

CFSP197

ELIZABETE APARECIDA GONÇALVES DO AMARAL1; DANIELE MICHELIN PAGANOTTE1; ADEMIR SALVI JÚNIOR2. Fho Fundaçao Herminio Ometto, Araras - SP - Brazil; 2Centro Regional Universitário de Espírito Santo do Pinhal - UNIPinhal, Espirito Santo do Pinhal - SP - Brazil. 1

Introduction and objective: Obesity has become a worldwide public health problem. Data from the IBGE (2015) show that about 82 million people are overweight or obese. After the ban on substances used in slimming: amphetamines, by ANVISA (RDC no. 52/2011), there has been a growing demand for alternative, promising and “effective” sources for the treatment of obesity. The herbal medicine Morosil, prepared with dried extract of Moro oranges (Citrus sinensis (L.) Osbeck - Family: Rutaceae) comes to this encounter, gaining prominence when it comes to weight loss. The use of this type of medication, most of the time, is used by self-medication or without professional follow-up, which can result in serious health problems. Pharmacotherapeutic follow-up of overweight or obese patients may be a strategy for patient adherence to the treatment used in this follow-up and corroborates the tendency that the treatment of obesity should be by a multiprofessional team. The objective of this study was to carry out the pharmacotherapeutic follow-up on users of the medicinal product Morosil, inserted in a practice of pharmaceutical care of a Magistral Pharmacy of Espírito Santo do Pinhal-SP. Materials and methods: This work is a cross-sectional descriptive quantitative study. The sample consisted of individuals randomly selected for convenience, who used the herbal medicine Morosil, with or without medical prescription, as single source of weight loss. The procedure began after the approval of the Ethics Committee in Research on Human Beings (CAAE: 66389417.6.0000.5385) and was carried out from May to July 2017. The data were collected through the questionnaire: Dáder Method of Pharmacotherapeutic Follow-up (MACHUCA; FERNANDEZ-LLIMOS; FAUS, 2003), adapted for the present study. Results and conclusion: The sample consisted of 35 individuals, of which 97.1% were female, with a prevalence of 40.0% with an age range of 40 to 49 years. It was observed that 100% of the individuals reported not being satisfied with their weight. They were evaluated, and classified, 51.4% as pre-obese and 34.4% as obese. They also reported other associated health conditions, such as endocrine and/or hormonal disorders (34.2%), psychiatric disorders (22.8%), cardiovascular disorders (14.2%) and others. Of the individuals analyzed, 96.8% use more than one medication per day, and regarding the use of Morosil, 91.4% used it without any kind of guidance and only 8.6% do so under professional follow-up. When analyzing the use of drugs, no drug interaction was found by association with Morosil. It can be said that the pharmacotherapeutic follow-up of the user allowed the maintenance of the therapeutic objectives and the promotion of the patient’s health, besides the professional recognition of the pharmacist. Financial support or fellowship: Naturallis Farmácia de Manipulação

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CFSP198 THE INCIDENCE OF HIV WITHIN THE MACROREGION OF SOBRAL-CEARÁ BETWEEN OCTOBER 2008 AND OCTOBER 2016. RAQUELINE DE ABREU MARQUES1; FRANCISCA IRACILDA SANTOS DO NASCIMENTO2; JEAN LINHARES DE LIMA3; PEDRO HENRIQUE MARTINS3. Uniara, Sobral - CE - Brazil; 2Inta, Sobral - CE - Brazil; 3Prefeitura Municipal de Sobral, Sobral - CE - Brazil.

Introduction and objective: While the USA registered its first confirmed AIDs case in while 1981, the state of Ceará followed suit in 1983, after which many patients fell victim. Up to 2006, 95% of the counties in Ceará had registered cases of HIV/AIDs. Objective: Characterize the incidence of HIV in the northern macroregion of the state of Ceará between October 2008 and October 2016. Materials and methods: Data was derived from the data system SICLOM, managed by the municipal council of Sobral. All notified cases during this interval (1st October to 30th September of the subsequent year) were computed. No personal data regarding the patients was taken into consideration. Results and conclusion: A mean of 155.5 cases were notified per interval/year, corresponding to an incidence of 14.58 per 100,000 habitants. The interval 2008-2009 posted the highest notification index. After 2013, the notification rate was considered ‘stable’. Conclusion: The incidence of HIV/AIDs in the macroregion of Sobral is lower than that of the Northeast Brazil, but higher than that of Ceará. This means strategies based on equity warrant elaboration in order to ease the HIV/AIDs burden in this region. CFSP199 PERCEPTION OF PATIENT SAFETY CULTURE IS ASSOCIATED WITH JOB SATISFACTION IN HOSPITAL PROFESSIONALS ALAN MAICON DE OLIVEIRA1; MARCUS TOLENTINO SILVA2; TAÍS FREIRE GALVÃO3; LUCIANE CRUZ LOPES1. São Paulo State University (Unesp), School of Pharmaceutical Sciences, Araraquara - SP - Brazil; 2University of Sorocaba (Uniso), Sorocaba - SP - Brazil; 3Campinas State University (Unicamp), School of Pharmaceutical Sciences, Campinas - SP - Brazil. 1

Introduction and objective: Evidence-based knowledge is necessary to consolidate the theory that organizational factors reflect the provision of health care services and affect the safety aspects of care. Therefore, the objective of this study was to evaluate the correlation between the perception of the patient safety culture and the hospital professionals work satisfaction. Materials and methods: This cross-sectional study was carried out in the second half of 2016 in a teaching hospital in the northwest of the State of São Paulo. The inclusion criterion involved all the hospital employees ≥ three months. Subjects were excluded who were on medical leave/withdrawn or on vacation. From a list of 677 hospital professionals, 305 were randomly selected. Data collection of the level of job satisfaction (Job Satisfaction Survey-JSS) were performed through validated instruments, as well as the relation of this factor in the perception of the patient safety culture (Safety Attitudes Questionnaire-SAQ). The data analysis was performed by using the Pearson correlation coefficient and the partial least squares structural equation modeling (PLS-SEM). A reliable gauge was set at 95% (95% CI) and the significance level at 5%.” Results and conclusion: Of a total of 305 selected professionals, 271 composed the final study sample (88.9%). Women (78.6%), aged between 36-50 years old (42.1%), nursing staff (46.1%) and 5-10 years working in the area (27.3%) were the most common characteristics. The SAQ average score was 68.2 (95% CI: 65.8-70.5) and a negative perception of safety culture was verified. The JSS had an average score of 131.2 (95% CI: 128.1-134.3) with 18.5% of job dissatisfaction. The correlation coefficient determined a considerable association between two variables (r = 0.69) Financial support or fellowship: National Council for Scientific and Technological Development (CNPq).

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PHARMACEUTICALS IN THE SUS: WOUND HEALING WITH PHYTOTHERAPICS

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CFSP200

NILSA SUMIE YAMASHITA WADT1; BIANCA CRISTINA DE MELLO JUNQUEIRA2; BRUNA MOTA CAMPOS2; DENISE HELENIR MACEDO2; DIANA DE SOUZA PETRONI2; FERNANDA CAVALINI3; THAIS FERNANDA PEDROSO SANT ANNA3; ERNA ELISABETH BACH4; MARCELO WADT1. UNIP, Valinhos - SP - Brazil; 2UNIP, Jundiai - SP - Brazil; 3Prefeitura de Valinhos, Valinhos - SP - Brazil; 4Uninove, São Paulo - SP - Brazil.

Introduction and objective: the use of phytotherapics in SUS is being encouraged by the government. The city of Valinhos-SP possesses a Vascular and Neuropathic Lesions Specialized Service (SELVEN), for treatment of varicose ulcers that are difficult to heal, leading to pain and discomfort for the patient. The objective from the present work was to evaluate the use of herbal phytotherapics (tannins group) at SELVEN for treatment of varicose ulcers, aiming for an improved skin healing. Materials and methods: The project was approved in the Human Ethics Committee (CAAE:60579916.7.0000.5512). Patients were separated into 2 groups (10 patients/group); control – bandages of normal dressing, and with phytotherapics- guava, red Brazilian berry and witch hazel (30 minute bath) leaves decoction. The evaluation of pain relief, odor, inflammation and wound area lasted for 6 months. In case of guava tea, an antioxidant assay was performed by the ABTS method (Trolox pattern). Results and conclusion: The patients treated showed marked decrease pain relief and odor, and the wounds had significant improvements, such as secretion reduction, and beginning of skin healing process, when compared to control. The reduction of direct costs was significant, for example, suspension of negative pressure dressing. The guava decoction presented IC50 trolox = 334 uM/g. The phytotherapics that contain tannins aid the treatment and skin healing of wounds. Financial support or fellowship: Santander; UNIP; Valinhos City Hall. CFSP201 THE ROLE OF THE CLINICAL PHARMACIST IMPROVING VENOUS THROMBOEMBOLISM PROPHYLAXIS IN A LARGE PRIVATE HOSPITAL JULIANA RIBEIRO DE ASSIS; RENATA DANTAS CORREIA; VINICIUS CESAR DA SILVA MOREIRA; JAQUELINE PILON DE MENESES. Hospital Santa Catarina, São Paulo - SP - Brazil. Introduction and objective: The coagulation system is a balance between pro-coagulant activity factors and inhibitors, when an imbalance occurs a thrombus can develop. Venous Thromboembolism (VTE) is one of the most important preventable causes of death in hospitalized patients. Objective: To evaluate the role of the clinical pharmacist in improving VTE prophylaxis through pharmacist interventions and to analyze medical acceptance of interventions. Materials and methods: A retrospective cohort study was conducted with VTE high risk adult patients according to the institutional protocol between January, 01, 2016 and December, 31, 2016. The VTE prophylaxis pharmacist interventions were analyzed. To determine the taxonomy of interventions we performed an adapted version of the National Coordinating Council for Medication Error Reporting and Prevention (NCC MERP). Results and conclusion: 3,974 VTE high risk patients had their prescriptions analyzed. We observed 143 (3.6%) reports related to the prophylaxis of VTE and the taxonomy of interventions were: noncompliance in the medication process (28.0%), dose omission (25.1%), wrong time (15.4%), improper dose (16.1%), wrong frequency (14.0%), drug reconciliation failure (0.7%) and wrong drug (0.7%). In all pharmacist interventions, there was 84% of medical acceptance. The performance of the clinical pharmacist in the prophylaxis of VTE high risk adult patients is extremely important, aiming to increase medical adherence to the institutional protocol and thus intending to minimize the risk of possible events related to the VTE.

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CFSP202 THE USE OF HERBAL MEDICINES IN THE TREATMENT OF ULCERS IN A DIABETIC FOOT: CASE STUDY. EMANUEL PATRÍCIO CASTELO BRANCO FERREIRA; CARLA LARISSA DE CASTRO VIEIRA; HELOÍSA ESTEVES GURGEL DO AMARAL. Universidade de Fortaleza, Fortaleza - CE - Brazil. Introduction and objective: The use of medicinal plants to cure diseases has evolved over the years. The search for the knowledge of medicinal plants characteristics and properties has been helping the development of drugs; thus our objective is to analyze the use of herbal medicines in the treatment of ulcers in a diabetic foot. Materials and methods: The analyses of the medical records of the patient M.S.B. female, 80 years-old who has type 2 diabetes. The patient shows a stage IV extensive venous ulcer with the presence of exudate and necrosis points in the right lower limb, the leg has been almost circulating for over 10 years. She is monitored by the Centro de Diabetes e Hipertensão do estado do Ceará (Ceara’s State Center for Diabetes and Hypertension) nursing team. It is there that the ulcer cleaning and dressing procedures are carried out. The treatment was administered with: a Kerlix® bandage, antibiotics, hydrogen peroxide, sprayed water, papain gel at 3 concentrations 10%,15% and 20%, an Unna® boot and copaiba oil. Results and conclusion: At the beginning of the treatment a Kerlix® bandage associated with an injectable antibiotic was used, because the patient did not respond efficiently to the oral treatment. A month after the start of the treatment hydrogen peroxide was associated to Kerlix® and water was also sprayed. This showed a greater reduction of exudate. Keeping the conduct for 4 months, in search of more efficient results, papain gel, in association with an Unna® boot, was introduced at a 10% concentration. This showed an improvement. After 5 months, bearing in mind the marked results of odor remission, shrinking of lesion extension (length x width x depth) and considerable diminishing of the hardened ridges (Lipodermatosclerosis), the papain gel concentration was increased to 15%. After two more months of weekly bandaging at the rigorous hygienization clinic, debridement and Unna® boot application associated alternatively with either copaiba oil or papain oil. It was possible to observe, over the period of 50 weeks in which the case was followed, the full healing of two-thirds of the lesion. Therefore the adopted conduct may be considered sound. In a short period of time, if the situation goes on in the way it is now, without harmful disturbances and with the patient’s compliance it is believed that success will be achieved with an ancient, low cost and effective technology. CFSP203 SELF-MEDICATION TREATMENT FOR HEADACHE: A CROSS-SECTIONAL STUDY WITH HEALTH STUDENTS FROM A UNIVERSITY CENTER IN THE SOUTH OF SANTA CATARINA STATE MARCO ANTONIO VALENTE1; FRANSCIELE MARGHETI FERNANDES1; ANA PAULA BAZO1; MORGANA MARIA CASCAES MONTANHA1; LEONARDO DE PAULA MARTINS2. Centro Universitário Barriga Verde - Unibave, Orleans - SC - Brazil; 2Centro Universitário Barriga Verde - Unibave, Orleans - Brazil. 1

Introduction and objective: Self-medication is characterized by the consumption of a product for the purpose of treating or alleviating symptoms or diseases, or even for the purpose of promoting health, regardless of prescription of a professional. Although self-medication constitutes an important form of population self-care, it presents inherent risks. In this context, this study aimed to describe the self-medication profile for the treatment of headache among health students of a higher education institution in the South of the Santa Catarina State. Materials and methods: A cross-sectional, descriptive study was carried out with 224 university students from a health area. The data was collected using a self-administered, structured questionnaire, to obtain information on self-medication in cases of headache. The survey was performed between September and October of 2016. The study variables included study characterization issues, issues related to the occurrence of headache and self-medication. The Research was approved by the Research Ethics Committee Involving Human Beings, Certificate No. 1,611,910. Ethical principles were respected in accordance with Resolution No. 466/2012 of the National Health Council. Results and conclusion: A high rate of self-medication among students was observed. Headache is a major cause of self-medication reported, 13 drugs used to treat headache were identified. The most frequently mentioned analgesics were paracetamol, dipyrone and ibuprofen. Medications containing combinations with opiates such as codeine and tramadol were also reported. From the results of this study, health education actions are suggested, since the interviewees did not recognize risk in the practice of self-medication.

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CFSP204 TRIAZOLES OR ECHINOCANDINS ANTIFUNGAL THERAPY AGAINST CANDIDA GLABRATA IN SEPSIS? VANESSA KASUBECK DE SOUZA1; CECILIA DOLORES DE FARIAS1; JOÃO MANOEL DA SILVA JR2; AMANDA MARIA RIBAS ROSA OLIVEIRA2; EDVALDO VIEIRA DE CAMPOS2; CARLINDO VIEIRA DA SILVA JR1; DAVID DE SOUZA GOMEZ2; SILVIA REGINA CAVANI JORGE SANTOS1. Fcf USP- Clinical Pharmacokinetics Center, School of Pharmaceutical Sciences, São Paulo - SP - Brazil; 2Hcfmusp-Division of Plastic Surgery and Burns, São Paulo - SP - Brazil. 1

Introduction and objective: Micafungin is a new semisynthetic echinocandin quite effective against the common fungal pathogens related to its high lipophylicity characteristics; while fluconazole is largely prescribed against Candida spp systemic infections. The aim of the study was to compare drug effectiveness in a burn patient based on PK/PD approach. Materials and methods: A burn patient, male, 71yrs, 74kg, 174cm (height), with etiology by fire 38% (TBSA), inhalation injury was admitted to the intensive care unit (ICU). Patient with sepsis suspicion after 48hs of hospital admission was treated with systemic vancomycin-piperacillin combined. Since therapeutic fail occurred, meropenem was prescribed against Acinetobacter baumannii MIC 8mg/L, and the pathogen was eradicated in ten days of treatment. Then, after two weeks patient received antifungal therapy with fluconazol 400mg daily and two days later up to 600mg. Two weeks after patient’s admission to ICU Candida glabrata was isolated MIC 32 mg/L/fluconazole resistant; micafungin susceptible MIC 0.032mg/L. Clinical team decided to treat the fungal infection with micafungin (100mg qhr24 for 21 days), s the patient was in renal failure. Drug plasma monitoring was performed by blood sampling; antifungal effectiveness was based predictive index AUCss0-24/MIC>100 for micafungin and AUCss0-24/MIC>25 for fluconazol. Results and conclusion: Micafungin pharmacokinetics is not altered in septic burn patient with renal dysfunction, consequently, no dose adjustment was required, as the therapeutic target was attained with the empiric dose recommended. Financial support or fellowship: FAPESP

USE OF MEDICINAL PLANTS BY HIV VIRUS BEARERS

CFSP205

WILLIAN CHARLES DA SILVA MOURA1; MIGUEL ANGELO SANTOS PINHEIRO SEGUNDO2; ÍTALA SAMARA DA SILVA DIAS1; GERALDO EDUARDO GUEDES DE BRITO3; ZILKA NANES LIMA1; RICARDO OLIMPIO DE MOURA1. Universidade Estadual da Paraíba, Campina Grande - PB - Brazil; 2Universidade Federal de Pernambuco, Recife - PE - Brazil; Universidade Federal da Paraíba, João Pessoa - PB - Brazil.

1 3

Introduction and objective: The use of Medicinal Plants by Human Immunodeficiency Virus (HIV) carriers may interfere with antiretroviral therapy. According to the literature, there is great potential for pharmacokinetic and pharmacodynamic interactions between Medicinal Plants and antiretroviral drugs. This study aimed to analyze the use of Medicinal Plants by HIV-Positive subjects participants in a Non-Governmental Organization in the city of Campina Grande - Paraíba. Materials and methods: A quantitative, exploratory and descriptive study, with 16 subjects of both genders, HIV virus carriers. The sample size was defined based on the information saturation criterion. The exploratory classification was performed according to the Medicinal Plants catalog. Data were collected through a semi-structured interview. Results and conclusion: The study population had a homogeneous distribution between the sexes (43.7% men, 56.3% women), concentrating on the age group over 50 years (56.3%) and the majority with more than 10 years of diagnosis (75%). Of the interviewees, 87.5% used traditional Medicinal Plants. A total of 25 plants were reported, and 5 were highlighted: Lippia alba Garden (16.9%), Peumus boldus Molina (11.2%), Allium sativum L. (9.3%), Cymbopogom citratus Stapf Mentha crispa L. (5.7%). Interactions were confirmed for 6 Medicinal Plants with emphasis on Allium sativum L and Peumus boldus Molina. The results pointed to the widespread use of Medicinal Plants and the need to promote health education activities together with the NGOs regarding their indiscriminate use and potential risks. Financial support or fellowship: PROAPEX; UEPB.

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CFSP206 USE OF METHYLPHENIDATE BY CHILDREN AND TEENAGERS DIAGNOSED WITH ATTENTION DEFICIT HYPERACTIVITY DISORDER AT PSYCHOSOCIAL SUPPORT CENTER – ITAQUERA, STATE OF SÃO PAULO MARCOS HENRIQUE CREVELARIO DOS SANTOS; CAMILA DOS SANTOS CARDOSO; CLEIDIANE DE SOUSA ROCHA; LUARA CRUZ FERNANDES; NATALIA RIBEIRO DOS SANTOS; MICHELE MELO SILVA ANTONIALLI. Universidade São Judas Tadeu, São Paulo - SP - Brazil. Introduction and objective: Methylphenidate is a mild central nervous system (CNS) stimulant indicated as an adjuvant to psychological, educational and social interventions in the treatment of hyperactivity disorders. Recently, the use of this stimulant by children and teenagers diagnosed with Attention Deficit Hyperactivity Disorder (ADHD) has increased significantly. Objectives: Researching the use of methylphenidate in the treatment of ADHD, verifying its main adverse reactions, dosage and conditions of use, as well parents or guardians’ expectations, satisfaction, doubts and difficulties regarding the treatment. Materials and methods: Questionnaires were applied to parents or guardians of children and teenagers treated with methylphenidate who have received treatment at the Children Psychosocial Support Center, in Itaquera, State of São Paulo - Centro de Apoio Psicossocial (CAPS Itaquera). Results and conclusion: 19 children and teenagers with an ADHD diagnosis, treated at CAPS Itaquera, use methylphenidate, their age range is between 1 and 17 years-old, and 13 of them are over 10. Preliminary results demonstrate that the parents’ main doubt is regarding the need to take the drug continuously. The study also identified a dispensation reduction of methylphenidate during school vacations at CAPS’s pharmacy.

USE OF PSYCHOTROPICS AMONG COLLEGE STUDENTS: PREVALENCE AND CORRELATES

CFSP207

RAPHAEL PEREIRA CASSAMASSIMO. Faculdades Metropolitanas Unidas, - Brasil. Introduction and objective: The use of psychotropic consists of a public health problem, but few studies have investigated the subject in university students in Brazil. This study aims to investigate the prevalence and factors associated with the use of psychotropic drugs in university students. Materials and methods: This is a cross-sectional study performed at a public university with students from the health area, approved by the Research Ethics Committee. Self-administered questionnaires were answered with questions about mental health and drug use, among others. The outcomes are use of benzodiazepines and use of antidepressants. The exploratory variables are age, sex, income and Common Mental Disorder, evaluated through the Self Reporting Questionnaire. The association between outcomes and explanatory variables was investigated through bivariate analysis and later logistic regression models were constructed. Results and conclusion: More than 80% of the students participated in the research. In the sample, 2.9% and 9.8% of subjects reported use of benzodiazepines and antidepressants, retrospectively. The use of antidepressants was associated with the presence of common mental disorder, even after adjustment for age, sex and income. The use of benzodiazepines was not associated with any of the variables investigated. It is noteworthy that the prevalence of use of both drugs is higher than in the general population and there is no gender interference. Personal and academic difficulties may account for this use. We need more studies in this population, particularly as they are students who will work in the health area when trained. Financial support or fellowship: Financial support: FAPESP 14/15872-6

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CFSP208 USE OF VITAMIN D AND CALCIUM IN THE PREVENTION OF BONE FRACTURES IN ELDERLY IN THE CITY OF GRÃO PARÁ, SC ALEXANDRE JOÃO MARTINS NETO 1; MARIANA KOCH PEREIRA 2; ANDRESSA CORNEO GAZOLA 3; ALEXANDRE PICCININI4; LEONARDO DE PAULA MARTINS4; ADALBERTO ALVES DE CASTRO3. Centro Universitário Barriga Verde - Unibave, Orleans - Brazil; 2Centro Universitário Barriga Verde - Unibave, Grão Pará - SC - Brazil; 3Centro Universitário Barriga Verde - Unibave, Criciúma - SC - Brazil; 4Centro Universitário Barriga Verde - Unibave, Orleans - SC - Brazil. 1

Introduction and objective: Complications of fractures are relevant clinical sequelae of osteoporosis, almost all fractures in the elderly are due to low bone density. To reduce the risk of fractures, several interventions are recommended for the general population. These include adequate intake of calcium and vitamin D, regular physical activity, daily sun exposure, avoiding the use of tobacco and alcoholic beverages. Thus, the present study aimed to evaluate the use of calcium and/or vitamin D in relation to the prevention and treatment of fractures in the elderly in the city of Grão Pará, SC. Materials and methods: The survey was conducted using a questionnaire with 50 seniors, who were divided into group 1 (participants without bone fractures) and group 2 (participants with bone fractures). It was noted in the study that in both groups the vast majority of participants were females. All participants used calcium supplement and/or vitamin D, of which the consumption of Fixed Cal stood out as the most used. Results and conclusion: Most seniors expose themselves to sunlight daily. In group 1, most of the participants practiced physical exercises regularly. The milk and dairy products intake prevailed in both groups, but we observed a frequency of greater consumption in group 2. Thus, we warn the population to control some factors involved in the development of this pathology, preventing bone fractures in the elderly to improve their quality of life. CFSP209 IS VANCOMYCIN THERAPEUTIC TARGET AGAINST STAPHYLOCOCCUS SPP MIC1MG/L ACHIEVED AFTER THE EMPIRICAL DOSE REGIMEN RECOMMENDED TO CRITICALLY SEPTIC PEDIATRICS? VEDILAINE APARECIDA BUENO DA SILVA1; JOÃO MANOEL DA SILVA JR2; EDVALDO VIEIRA DE CAMPOS3; CARLINDO VIEIRA DA SILVA JR4; DAVID DE SOUZA GOMEZ3; SILVIA REGINA CAVANI JORGE SANTOS4. Faculdade de Ciências Farmacêuticas da USP, São Paulo - SP - Brazil; 2Hcfmusp-Division of Plastic Surgery and Burns, Sao Paulo - SP - Brazil; 3Hcfmusp-Division of Plastic Surgery and Burns, São Paulo - SP - Brazil; 4Fcf USP- Clinical Pharmacokinetic Center, School of Pharmaceutical Sciences, São Paulo - SP - Brazil. 1

Introduction and objective: It is well known that pharmacokinetics is altered in a different manner for vancomycin in septic critically ill pediatrics compared to adult patients. Vancomycin effectiveness must be guaranteed by the area under the curve/MIC ratio (AUCss0-24/MIC) instead trough plasma levels largely applied to dose adjustment routinely. Thus, the objective of the case report was to evaluate whether the empiric dose regimen recommended achieves the target against gram-positive MIC>1mg/L strains or not based on the pharmacokinetic-pharmacodynamic (PK/PD) approach. Materials and methods: Pediatric burn patient: F, 3 yrs, 18kg body weight, 18% total burn surface area was investigated. Vancomycin was administered 10 mg/kg every 6 hrs, one hour pump infusion, 40mg/kg daily. PK/PD target was based on drug effectiveness index recommended AUCss0-24>400. Hemoculture and susceptibility testing were performed in the central laboratory. Blood was sampled (2 mL/each) at the 2nd and 4th hr of the end of drug infusion, and vancomycin plasma measurement was performed by liquid chromatography. Daily dose should be increased if therapeutics failed. Results and conclusion: Trough plasma level was lower than expected after daily dose 40mg/kg with target attainment just against MIC 0.5mg/L strains, while PK/PD target was attained up to MIC 2 mg/L when daily dose was 80mg/kg day. Consequently, Staphylococcus aureus (MIC 1mg/L) and Staphylococcus coagulase negative (MIC 2mg/L) were eradicated in 5 days of dose adjustment. Vancomycin plasma monitoring based on PK/PD approach done in a real time permits an earlier clinical intervention with good clinical outcome with cure of infection in a short period of vancomycin treatment. Financial support or fellowship: FAPESP

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CFSP210 VERIFICATION OF PHARMACEUTICAL CARE, MEDICATION THERAPY AND LIVING HABITS OF ELDERLY PATIENTS AT A CARDIOLOGY CLINIC IN JUNDIAÍ-SP. SABRINA DE ALMEIDA MARQUES1; DANIELA NASCIMENTO CARDOSO DOS SANTOS2; NATÁLIA CASTANHA DA SILVA1; APARECIDA ERICA BIGHETTI RIBAS1; VERONICA CRISTINA GOMES SOARES1. Centro Universitário Padre Anchieta, Jundiaí - SP - Brazil; 2Centro Universitário Padre Anchieta.

Introduction and objective: According to the World Health Organization (WHO), cardiovascular diseases are the leading cause of death in the world. The disease has a genetic cause, but nowadays it is more linked to incorrect diet and incorrect living habits. In this pathology, several types of drugs are used, and there are also drug interactions that can be observed during pharmaceutical care. In the late 1980s, Hepler began several studies on pharmaceutical care. Pharmaceutical Care is a follow up that the pharmacist does with his patients to identify any type of problem they are having with the medicines, aiming at improving the treatment of the patient. The objective of this study was to verify which medications elderly patients use the most, their habits towards their health and if they receive pharmaceutical attention when they acquire their medications and make correct use of their medication, understanding its importance and the reason why they use it. Materials and methods: The methodology was the application of a questionnaire to 38 elderly patients attending a Cardiology Clinic in Jundiaí-SP. This project was submitted to the ethics committee of Unianchieta following the Resolution CNS n. 466/12 and was approved. Results and conclusion: Most of the survey volunteers ranged in age from 60 to 70, about 52.63%, 31.57% from 71 to 80 and 15.78% above 80 years old. In addition, 15.80% of patients consume alcohol; 2.63% are smokers, only 26.31% say they practice physical activity. It was verified that 42.10% are dieting and it is known that food control contributes to therapeutic efficacy. Losartan, Hydrochlorothiazide and Sinvastatin are the most commonly used drugs by the volunteer group. More than half of the volunteers (about 60%) are hypertensive, 74% only use medications prescribed by the doctor while 26% said they self-medicate. When purchasing the drug, only 42% of them claim to seek the pharmacist for clarification of doubts, and the majority receive this information from the doctor. All the volunteers said they knew why to take and how to take the medication. Seeking pharmaceutical care in this case would help to have more benefits and effective therapy.

Brazilian Journal of Pharmaceutical Sciences

Area 2 Industry and Technology

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CFSP211 A PILOT STUDY OF THE CYTOTOXICITY OF HYDROETHANOLIC EXTRACT OF JURUBEBA IN MCF-7 BISMARQUE SOUZA PEREIRA1; KLINSMANN CAROLO DOS SANTOS2; JÉSSICA LEITE GARCIA2; VICKELINE NAMBA ANDRIOCIOLI2; DENISE FECCHIO2; IGOR OTÁVIO MINATEL3; GIUSEPPINA PACE PEREIRA LIMA3; CAMILA RENATA CORREA2; ANA PAULA COSTA RODRIGUES FERRAZ2. Faculdade Sudoeste Paulista- Fsp, Avare - SP - Brazil; 2Department of Pathology, State University Paulista Júlio de Mesquita Filho- Unesp, Botucatu - SP – Brazil; 3Department of Chemistry and Biochemistry State University Paulista Júlio de Mesquita Filho- Unesp, Botucatu - SP - Brazil. 1

Introduction and objective: Solanum paniculatum L.(Jurubeba)was a medicinal plant the leaves and roots of which have been used by the popular medicine on the treatment of gastrointestinal tract irregularities, however, there were no studies referring to the potential antioxidants results in biological models. In search of effective supporting treatments, initial cytotoxicity tests are required. Objective: evaluations of differents dosages and times in human adenocarcinoma mammal cells (MCF-7). Materials and methods: Approximately 2x105 cells were incubated on plates of 24 wells for 40 hours in culture medium RPMI 1640 with supplements and were maintained at 37ºC and atmosphere of 5% of CO2. Around 100g of dry fruit was prepared in accordance with the Brazilian Homeopathic Pharmacopeia (2011) and it was diluted in the culture medium RPMI 1640 (FDA, 2016), at concentrations of 5,10 and 50 μg/mL and untreated control group. The treatments were started at 24h and 72h times in triplicate and subsequently cytotoxicity was performed according to the MTT test (MOSMANN, 1983). The evaluations were performed according to mean standard deviation, providing % cell viability (%VC). Results and conclusion: The results obtained demonstrated toxicity dose and time dependent on their respective concentrations at 24h %VC of 84.21 (CV= 5.84), 13.11(CV=3.19), 11.39(CV= 1.19) and at 72h: 30.09(CV= 5.064), 11.14 (CV= 7.71), 11.62(CV=5.53)in comparison with control group = 100 (CV=5.38) The results obtained in the pilot study suggest that the doses/ times treatments should modified to attempt to find safety and efficacy. Financial support or fellowship: CNPq (Process: 130379/2016-6)

ACUTE TOXICITY STUDIES OF CONDIMENT PLANT CORIANDRUM SATIVUM L.

CFSP212

KÁTIA OLIVEIRA RIBEIRO; VICTÓRIA ALBUQUERQUE FERREIRA DA SILVA; ANDREIA REIS FABIANO; TAMARA SIQUEIRA PEREIRA LOPES; BIANCA CRISTINE DE OLIVEIRA; DANIEL MORENO GARCIA; VALERIA MARIA DE SOUZA ANTUNES; CARLOS ROCHA DE OLIVEIRA; ALEXANDRE MASSAO SUGAWARA. Universidade Anhembi Morumbi, São Paulo - SP - Brazil. Introduction and objective: Coriander, Coriandrum sativum L., a plant native to the east of the Mediterranean and west of Asia, is widely used in Brazilian popular cuisine as a condiment. It has carminative, digestive, antipyretic, anthelmintic, analgesic, antioxidant, anti-inflammatory, diuretic and antimicrobial properties. However, toxicity studies cannot be neglected. Toxicity study using microcrustaceans Artemia salina has been widely used as an indicator of toxicity. The study with Artemia salina has been correlated with antimicrobial activity and previous evaluation of possible antitumor properties. The utilization of human cells culture in acute toxicity has also been tested in fibroblastic culture. This work aims to evaluate the cytotoxic activity and potential antitumor in alcohol extract of Coriandrum sativum L. Materials and methods: Ethanolic extract of sheets of Coriandrum sativum L. (1:1) are diluted (10, 100, 1000, 1500 and 3000 ppm) and incubated with Artemia salina for 24hours after which the survival of culture was calculated. To obtain the LC50 values, the PROBIT analysis was used, with 95% confidence. Ethanolic extract of Coriandrum sativum L. (1:2) was tested in fibroblast cell line CCD1027Sk (ATCC) and cytotoxic activity was not observed. Results and conclusion: The 50% lethal concentration for Coriandrum sativum L extract was LC50 1045.41 μg/mL, considered to be threshold of toxicity effects, according to Meyer et al. (1982). The results of fibroblast cell line showed toxicity only at high concentrations, providing a survival rate of around 80-90% at doses close to LC50. In this sense, it is considered that coriander has potential for the prospection of antitumor effects with a wide therapeutic margin. We hope this study can serve as a source of further research.

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CFSP213 ACUTE TOXICOLOGICAL STUDY OF ETHANOLIC EXTRACT OF RHIZOMES OF ZINGIBER OFFICINALE CLAUDIA BUZI; FABRICIO DONÉ; FERNANDA BARBOSA; JAQUELINE BRICHES; LAIZ DE LIMA; ALEXANDRE MASSAO SUGAWARA. Universidade Anhembi Morumbi, São Paulo - SP - Brazil. Introduction and objective: Ginger rhizomes, Zingiber officinale, a plant native to South-East Asia, is widely used in Brazilian popular cuisine and medicine. The Pharmacotherapeutic Formulary of the Brazilian Pharmacopoeia (2011) recognizes its medicinal use, indicating its use as antiemetic, anti-dyspeptic, expectorant and in cases of motion sickness. Ginger has been associated with a larvicidal (Gomes et al.,2016) and antibacterial effect (Jain et al., 2015; Majolo et al, 2014; Andrade, 2012). Despite its recognized therapeutic effects, toxicity studies cannot be neglected. The toxicity study using microcrustaceans Artemia salina has been widely used as an indicator of toxicity. The study with Artemia salina has been correlated with antimicrobial activity (Moreira et al.,2003; Noldin et al.,2003; Zuque et al.,2004) and previous evaluation of extracts of plants with possible antitumor properties (Garcez et al., 2005; Ngoumfo et al.,2010; El-Menshawi et al., 2010). This work aims to evaluate the cytotoxic activity and potential antimicrobial and antitumor in alcohol extract of ginger. Materials and methods: Ethanolic extract of ginger rhizomes is diluted and incubated with Artemia salina. To obtain the LC50 values, the PROBIT analysis was used, with 95% confidence. Results and conclusion: The 50% lethal concentration for ginger extract was LC50 3287.67 mcg/mL, considered to be of low toxicity, according to Meyer et al. (1982). In this sense, it is considered that ginger has potential for the prospection of antimicrobial and antitumor effects with a wide therapeutic margin. We hope this study can serve as a source of further research. CFSP214 ANALYSIS OF KNOWLEDGE OF ORGANIC FOODS IN A POPULATION IN THE CITY OF CAJAZEIRAS-PB ANNALU MOREIRA AGUIAR; ANNA CLAUDIA MOREIRA AGUIAR; TALITA DI PAULA MACIEL BRAGA QUIRINO; ANA EMÍLIA FORMIGA MARQUES; NATÁLIA BITÚ PINTO. Faculdade Santa Maria, Cajazeiras - PB - Brazil. Introduction and objective: Organic agriculture arises with the aim of providing food to the population with guaranteed lack of chemical residues and food safety, since the use of pesticides can pose serious risks to the health of those who consume them, such as poisoning, risks of teratogenicity, mutagenicity and carcinogenicity. It was proposed to research the socioeconomic level and knowledge about organic foods from 52 clients of a fruit and vegetable store located in the city of Cajazeiras in Paraíba in the year 2012. Materials and methods: A semi-structured questionnaire was used, with objective and subjective questions based on the questionnaire used by Borguini (2002). Data analysis used the program Microsoft Office Excel 2007 with the determination of the averages and the standard deviation of the results. Results and conclusion: Of the surveyed population, 42% were male and 58% female, 81% of the respondents have some knowledge and 19% do not know, do not remember or know very little about it. The TV was the main source of obtaining this information, followed by the internet and information exchanged between friends and family. Even if many know about organic foods, 58% of respondents said they do not consume it and the 35% that do consume, do it to obtain a better quality of life because they know they are free of pesticides and prefer the products’ taste. Therefore, it is necessary to create public policies for investment in organic agriculture and encourage the population to consume these products. Availability of seminars and lectures is also a good strategy for making information available to farmers and the population.

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CFSP215 ANALYSIS OF PHYTOCHEMICAL PROFILE AND ANTIOXIDANT METAL CONTENT IN MYRCIARIA CAULIFLORA (JABOTICABA) PEEL EXTRACTS DESTINED FOR COSMETIC ACTIVITY NICOLE DE ALMEIDA NUNES MEIRA; NEILA DE PAULA PEREIRA; LEONARDO FONSECA MACIEL; JOSÉ ANTÔNIO MENEZES FILHO; SÉRGIO SOARES DO PRADO OLIVEIRA. Universidade Federal da Bahia, Salvador - BA - Brazil. Introduction and objective: Fruticulture has become increasingly prominent in Brazil. Because of that, it has a high-income multiplier effect, boosting local economies that do not have many development alternatives. In this context, the Brazilian fruit Myrciaria cauliflora is characterized by being rich in phenolic compounds and with good antioxidant activity, mainly the extracts of its peels. Objectives: analyze the phytochemical profile and the content of antioxidant metals in jaboticaba peels extracts, destined for use in cosmetics. Materials and methods: The hydroglycolic (HG) and hydroethanolic (HE) extracts were obtained by maceration following percolation. The content of total phenolic compound (TPC) and antioxidant activity by Ferric Reducing Antioxidant Power (FRAP), were measured. The determination of iron (Fe) and manganese (Mn) in peels in natura and after extraction was performed by graphite furnace atomic absorption spectrometry. Anionic and non-ionic emulsions were prepared to which extracts were added and submitted to accelerated stability tests. Results and conclusion: The HG presented the best result of TPC (HG= 4922,2 mg GAE/100g; HE= 4750 mg GAE/100g) and also for FRAP (HG= 27.16 µM FeSO4/g; HE= 22.24 µM FeSO4/g) besides presenting a higher amount of Fe and Mn, since they were in lower concentration in the peels after extraction. After the accelerated stability tests all emulsions remained stable. Despite the better performance of the HG, both extracts proved to be a bioproduct suitable for cosmetic use, adding sustainable value to this fruit and should be studied more deeply. Financial support or fellowship: FAPESB. CFSP216 ANALYSIS OF RADIOPHARMACEUTICALS TRACED WITH FLUORINE-18 AND ITS INTERACTIONS ANDREYA GONÇALVES COSTA MOTTA1; MERYCK FELIPE BRITO DA SILVA1; BRUNA CRISTHYNE DE DEUS ROSA1; FERNANDA CORRÊA CRUZ1; DANUZA ESTÉLA MÜLLER2; VINICIUS BARRETO DA SILVA1. Pontifical Catholic University of Goiás, Goiânia - GO - Brazil; 2Nuclear Medicine Institute, Goiânia - GO - Brazil.

Introduction and objective: Radiopharmaceuticals are molecules connected to radionuclides or radioisotopes, by which constitute radioactive drugs used for diagnostic with the purpose of visualizing the organ or system anatomy and/or used in therapies with the intention of irradiating internal tissues of the organism in Nuclear Medicine. The main goal of this study was to quantify the radiopharmaceuticals that do not have defined biological targets and highlight the need for further studies correlating their place of action and use and application in Nuclear Medicine. Materials and methods: The present study was performed in a data bank, with the support of the DIRAC - 18F dataset in which all the radiopharmaceuticals present in this database, a total of 1312, were analyzed according to their occurrence, that is, the absolute value of radiopharmaceuticals that act in each receiver; the subtype of each receptor and the frequency or relative value of that relation, allowing the distribution of the biological and chemical space of the radiopharmaceuticals to be determined. This study had discrete quantitative inferential statistical analysis with graphical representation by means of a frequency polygon. Results and conclusion: About 12.12% of fluorine-18-labeled radiopharmaceuticals presented unknown biological receptors, however, they are widely used in Nuclear Medicine, studies on specific biological receptors are needed for the planning of structural alterations that help increasing the affinity of the ligand to the biological target. In this way, it allows the prevention of drug interactions of radiopharmaceuticals with food and other drugs used in other therapies. Such information may contribute to the compilation of new guidelines for the use of radiopharmaceuticals and to decrease the degree of toxicity to healthy tissues, as well as contributing to the early diagnosis of pathologies. Financial support or fellowship: FAPEG

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CFSP217 ANTIBACTERIAL ACTIVITY OF COPAIBA OIL AGAINST PLANKTONIC AND BIOFILM CELLS OF STREPTOCOCCUS AGALACTIAE ANA ELISA BELOTTO MORGUETTE1; RENATA DE LIMA VARELLA2; RENATA PERUGINI BIASI-GARBIN2; ELIANE SAORI OTAGUIRI2; LUCY MEGUMI YAMAUCHI2; SUELI FUMIE YAMADA-OGATTA2. Unifil, Londrina - PR - Brazil; 2Universidade Estadual de Londrina, Londrina - PR - Brazil.

Introduction and objective: Streptococcus agalactiae (Group B Streptococcus – GBS) is a leading cause of invasive infections in neonates and intrapartum antimicrobial prophylaxis (IAP) remains an important measure for infection control. However, resistance to antibacterials used for IAP (penicillin, erythromycin, and clindamycin) has been described worldwide, indicating a need to search for new antimicrobials. Natural products are a rich source of potential antimicrobial molecules; therefore, the aim of this study was to assess the inhibitory activity of copaiba oil against planktonic and biofilm cells of GBS. Materials and methods: S. agalactiae ATCC 13813 and 9 GBS strains (4 erythromycin and/or clindamycin-sensitive and 5 -resistant strains) isolated from vaginal-rectal swab from women of reproductive age were employed in this study. Copaifera officinalis oleoresin was obtained commercially (Ferquima, Brazil). The minimum inhibitory and bactericidal concentrations (MIC and MBC, respectively) of copaiba oil on growth of planktonic GBS cells were determined by broth microdilution assay and colony forming unit counts, respectively. Established GBS biofilms (24 h) were treated with different concentrations of copaiba oil and the metabolic activity of sessile cells were quantified by 2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT)-reduction assay after 24 h of treatment. Results and conclusion: The MIC and MBC values were 0.03 and 0.06 mg/mL, and 0.06 and 0.12 mg/mL, respectively. Except for one GBS strain whose biofilm was not inhibited at any copaiba oil concentration tested, the metabolic activity of all biofilms was significant Financial support or fellowship: CNPq, CAPES, Fundação Araucária.

ANTIOXIDANT ACTIVITY OF JAMS WITH HERBAL POTENTIAL

CFSP218

DANIELE SILVA DOS SANTOS; MAYARA LETICIA DE SOUZA; JESSICA APARECIDA SOBRINHO SILVA; CYNTHIA GISELE DE OLIVEIRA COIMBRA. Centro Universitário Tabosa de Almeida (Asces-Unita), Caruaru - PE - Brazil. Introduction and objective: The incorporation of active extracts beneficial to health is an alternative to incitement to their daily consumption. Therefore, functional extracts and jams were evaluated according to their antioxidant potential and concentration, also evaluating the stability of such potential and the antioxidant activity, in the crude extract, such as in the produced jam. Materials and methods: As an experimental quantitative study, extracts of peppermint (Mentha sp.), basil (Ocimum basilicum L.), pepper (Capsium sp) and star anise (Illicium verum) were produced and then used in the production of jam, that was also later submitted to the same analyses, in which 3 ml of the sample (1 mg/ml) plus 1 ml of DPPH (0.1 mM) were separated in 95% of ethanol. This mixture was stirred for 30 min in an environment without light, following which the solution (sample + DPPH) was taken for analysis in the spectrophotometer (517 nm), and a calculation was made for the antioxidant activity, in which the equation of the line determined for the stretch of the calibration line was constructed with different concentrations of BHT (butylated hydroxytoluene), which is the standard antioxidant. Results and conclusion: 8 samples were put into analysis, 4 of which were crude extracts and 4 of the jams produced with these extracts, using the spectrophotometer, and the following results were obtained for the corresponding concentrations: 0.1M, 0.2M, 0.5M, 1.0M and 2.0M, with absorbance of, respectively: 1.318; 1.312; 1.290; 1.284; 1.280. The importance and the stability of the sequence of these results, such as the antioxidant activity, is noticeable making necessary further research to continue improving so we can offer other antioxidants in alternative to BHT and BHA, which are widely used.

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CFSP219 ANTIOXIDANT ACTIVITY OF THE BRIGADEIRO SWEET MADE WITH EXTRACT OF PEPPERMINT (MENTHA PIPERITA) THAYNARA BRUNO PRIM REIS1; ADJA LUCIVÂNIA DA SILVA2; BRENDAA EMMOLY DE OLIVEIRA2; CARLA VALÉRIA DE MELO2; JESSICA APARECIDA SOBRINHO SILVA2; JOSÉ SAMUEL DE LIMA2; MAYARA LETICIA DE SOUZA2; CYNTHIA GISELE DE OLIVEIRA COIMBRA2. Centro Universitário Tabosa de Almeida, Caruaru - PE - Brazil; 2

Introduction and objective: The consuming of foods or natural antioxidant ingredients help in the maintenance of health by reducing the likelihood of cardiovascular disease and cancer. However, the taste is not always well accepted and adherence to preventive treatment can be compromised. Placing them in foods with good sensory acceptability, such as sweets, is a way to promote ingestion and to accustom them to the taste of these ingredients. It is possible to include these ingredients in a formulation that modifies their properties by interacting with the other constituents and therefore it cannot be said that the activity observed for the functional ingredient will be maintained wholly when included in a food. The distribution of Mentha piperita brigadeiros is standard practice at events and presentations of the Pharmacists of the Future Extension project of the ASCES UNITA Pharmacy Course. In view of the positive impact due to its differentiated flavor, the beneficial properties conferred by the consumption of M. piperita, and the possibility of modifying these properties by including it in a formulation, this work aimed to determine and compare the antioxidant activity of M. Piperita juice and brigadeiros. Materials and methods: The M. piperita juice was extracted by pressing the leaves of the vegetable and reserved under refrigeration during the preparation of the mass of the brigadeiro. To prepare the brigadeiros, 140 mL of condensed milk were used, which was heated in pan until it was released from the bottom of the pan, without draining when it was inclined. The mass was cooled to room temperature and then 10 mL of M. piperita juice was added, homogenized and proceeded the measures. The brigadeiros was diluted to different concentrations and its antioxidant activity determined by the DPPH method. The dilution absorbance, which was found in the straight line of the calibration curve constructed with BHT, was used to calculate the antioxidant activity Results and conclusion: The antioxidant potential of M. piperita juice was 100% and, the brigadeiro was of 21.0%. Considering that the dilution of juice in the production of brigadeiros was inferior to 6.7%, it was possible to verify that the antioxidant activity did not accompany the reduction of the concentration. This was possibly due to the presence of lactose and partially inverted sucrose (reducers) present in the brigadeiro mass, which increased the antioxidant potential of M. piperita juice. These results allow us to affirm that the use of peppermint juice in brigadeiros is viable and healthy due to its antioxidant activity and the consumption of peppermint brigadeiros contributes to the reduction of diseases stimulated by the presence of free radicals. CFSP220 ANTISSEPTIC ACTIVITY AND STABILITY EVALUATION OF LIQUID SOAP WITH ADDED OCIMUM BASILICUM (L.) EXTRACT ANDRÉIA DE HARO MORENO; VICTÓRIA LARISSA DOS SANTOS; SÔNIA REGINA DE OLIVEIRA BERTOLUCI. Fipa, Catanduva - SP - Brazil. Introduction and objective: The use of medicinal plants for the treatment of diseases is associated with the folk medicine of different parts of the world. Several studies show that the essential oil of basil (Ocimum basilicum L.) presented antimicrobial activity against many Gram positive and Gram negative bacteria, fungi and yeasts. Due to its broad antimicrobial spectrum antiseptic activity and natural preservative, which makes it a potential active substance to be added in dermatological preparations, the aim of this study was to develop and evaluate the physicochemical stability and microbiological antiseptic activity of liquid soap with added basil extract. Materials and methods: The extract was prepared by turbolise technique using hydroalcoholic solvent according to the Brazilian Pharmacopeia. Then the extract was added to the liquid soap in concentrations of 5%, 10% and 20% and the samples were subjected to exposure to sunlight and temperature (ambient and refrigerator) for a period of four weeks. For the antimicrobial assay, the extract and the samples were evaluated against the following microorganisms: Staphylococcus aureus, Escherichia coli and Candida albicans, through the agar diffusion method. Results and conclusion: The results showed that the basil extract showed growth inhibition for microorganisms, the most significant action at a concentration of 20%. The results also indicated that light and temperature can affect the stability of the formulation, since it presented organoleptic changes and antimicrobial activity from the second week of exposure. Thus, the results show that basil extract may represent a viable alternative for obtaining new antiseptic agents. Financial support or fellowship: FIPA.

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CFSP221 APPLICATION OF A NONREGULAR DESIGN TO SCREEN EXCIPIENTS FOR LIDOCAINE-LOADED NLC VIVIANE LUCIA BERALDO-DE-ARAUJO1; ANDERSON BERALDO-DE-ARAUJO2; JULIANA SOUZA RIBEIRO COSTA1; LIGIA NUNES DE MORAIS RIBEIRO1; ENEIDA DE PAULA1; LAURA DE OLIVEIRA NASCIMENTO3. Department of Biochemistry and Tissue Biology, Biology Institute, University of Campinas, Campinas - SP - Brazil; 2Center for Natural and Human Sciences, Federal University of Abc, São Bernardo do Campo - SP - Brazil; 3Faculty of Pharmaceutical Sciences, University of Campinas, Campinas - SP - Brazil.

Introduction and objective: Nanostructured Lipid Carriers (NLC) are a recent generation of lipid nanocarriers, made of a solid lipid and at least one oil and one surfactant. In general, researchers do not present systematic criterion for choosing among the many possible lipids to produce NLC. The nonregular Hall design provides information with high resolution and low cost required, which allows a statistical analysis to compare and select lipids to produce NLC. The advantages of this system are biocompatibility, reproducibility and an efficient capacity to load hydrophobic drugs, such as lidocaine (LDC). The objective is to apply the Hall nonregular design to screen lipids and surfactants for LDC-loaded NLC. Materials and methods: Screening of lipids: the lipids were selected according to their origin, melting temperatures, drug solubility. NLC production: NLC was produced using selected vegetable lipids and super refined oils by hot homogenization method. NLC characterization: Average particle size (ZA), polydispersity index (PDI), zeta potential (ZP) of the NLC and entrapment efficiency (%EE) were measured. Statistical analysis: normal, main effects and second order interactions were calculated with R language. Results and conclusion: The main factors were: ZA: polysorbate-80, LDC and cetyl palmitate; PDI: cetyl palmitate, beeswax, polysorbate-80 and Kolliphor; ZP: polysorbate-80, LDC and sesame oil; %EE: cetyl palmitate, beeswax, capric/caprylic oil, sesame oil and corn oil. After a linear regression of a second order polynomial, the main factors exhibited multiple R-squares > 0.7 and p-values < 0.05. The main conclusions are that the selected excipients, on average, presented the desired characteristics for LDC-loaded NLC, but cetyl palmitate, sesame oil and Kolliphor have negative influence on ZA, %EE and PDI, respectively. Financial support or fellowship: CAPES; FAPESP. CFSP222 APPLICATION OF CHROMATOGRAPHIC PROPERTIES IN THE USE OF DERIVATIZERS FOR IDENTIFICATION OF AMINO ACIDS IN REVERSED-PHASE COLUMNS BY HPLC EMMANUELA PRADO DE PAIVA AZEVEDO1; NATÁLIA MILLENA DA SILVA2; MARCELLA MELO ASSIS COSTA2; CLAYTON ANDERSON DE AZEVEDO FILHO3. Universidade Federal Rural de Pernmabuco, Recife - PE - Brazil; 2Universidade Federal de Pernambuco, Recife - PE - Brazil; Centro Universitário Tabosa de Almeida Asces-Unita, Caruaru - PE - Brazil.

1 3

Introduction and objective: Amino acids are ionizable molecules, a property on which the chromatographic methods are based for separation. Ion-exchange chromatography has this purpose, but due to its high cost, reversed-phase chromatography has been used. Amino acid analysis is applied in areas such as proteomics, food, animal feed and medicine, in which it is necessary to hydrolyze the material and derivative with substances such as orthophthaldehyde (OPA) and 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC). The objective of this work is to develop chromatographic conditions for derivatization of amino acids using OPA and AQC derivatizers. Materials and methods: The derivatization study was performed using OPA Sigma Aldrich ™ purity grade <0.99% and AQC. In the two derivation conditions, the detection was performed by fluorescence with excitation wavelength at 250 nm and emission at 395 nm. Column oven of 40 °C, injection volume of 5μL for AQC analyses and 10μL for OPA analyses and a flow of 1.0 mL/ min for all conditions. Results and conclusion: Through the methodology of Bartolomeo and Maisano (2006) for identifying amino acids, a new gradient condition was applied to the mobile phases: potassium phosphate buffer pH 7.8 (A), ternary mixture MeOH/ACN/H2O in the ratio 45:45:10 v/v/v (B) and ultrapure water (C) which remained fixed at 50% while mobile phases A and B were altered during the 43 minutes of running. The AQC analysis followed the gradient described in the AccQ Tag chemistry Package instruction manual and showed no chromatographic reproducibility in terms of separation and resolution. Financial support or fellowship: CNPq

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CFSP223 A-QbD APPLIED TO THE DISSOLUTION METHOD DEVELOPMENT OF MOXIFLOXACIN HYDROCHLORIDE 400 mg COATED TABLETS ANDERSON THIAGO CONSTANTINO; FERNANDO HENRIQUE PASCOTI BRUNH; MĂ RCIA CRISTINA BREITKREITZ. Unicamp, Campinas - SP - Brazil. Introduction and objective: The concepts of Quality-by-Design (QbD) and Process Analytical Technology (PAT) guidelines introduced by the Food and Drug Administration (FDA) in 2004 represented a significant step in the paradigm shift of conventional pharmaceutical manufacturing. It was later extended to the development of analytical methods (Analytical Quality by Design, A-QbD). The scientific literature on A-QbD (Analytical Quality by Design) for dissolution is scarce. Dissolution analytical methods can be used to establish in vivo and in vitro correlation (IVIVC) during the development of a generic drug, and also, they are important quality control tools capable of indicating significant changes that may affect the performance of the medicine (changes of process and formulation). Challenging the concepts of OFAT (One Factor At a Time) for the development of dissolution methods commonly used in pharmaceutical industries, this work aims to discuss new methodologies to the development of dissolution methods, in order to bring quality and efficiency in analytical development for pharmaceutical industries. Materials and methods: The solubility, stability and the use of filters were evaluated for dissolution media at pH1.2, 4.5 and 6.8 using a stability indicating method prior to A-QbD strategy application. The Critical Quality Attributes (CQAs) of method were defined and then a risk assessment for the experimental variables (CPPs, Critical Process Parameters) was elaborated. The high risk CPPs apparatus, speed of the and media volume were studied by a Design of Experiments (DOE) approach using Design Expert v9. The levels of the CPPs were: apparatus (paddle/basket), speed of the apparatus (50rpm/100rpm), media volume (250mL/750mL), studied at the three abovementioned pH media. Samples were collected at: 2, 5, 10, 15, 30 and 45 minutes. The percentage of the drug solubilized each time was quantified using a UV-Vis detector at 295nm. A commercial reference product and three pilot batches were studied. Results and conclusion: The half-normal probability graph showed the factors that most influence the dissolution are: apparatus, speed and the interaction between them, which indicated the need for a multivariate approach for optimization. Volume presented a minor effect and it was more pronounced with paddle apparatus and 50 rpm than with the paddle with 100 rpm and presented no effect whatsoever with the basket apparatus. Models were built using Multiple Linear Regression and evaluated by ANOVA, residuals and the graphic of predicted vs actual values and then response surfaces were built for each retention time. Response surfaces showed a linear model in the beginning of dissolution and quadratic behavior for further times. Based on response surfaces obtained, it was possible to select conditions for a discriminative and robust dissolution method. As the main conclusion, it was demonstrated that A-QbD provided a rational approach for method development, providing scientific justification for the establishment of method specifications.

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CFSP224 CAPILLARY ELECTROPHORESIS: A GREEN TOOL FOR THE ANALYSIS OF DRUGS – APPLICATION TO INJECTABLE DAPTOMYCIN ELIANE GANDOLPHO TÓTOLI1; CRISTIANE MASETTO DE GAITANI2; HÉRIDA REGINA NUNES SALGADO1. Universidade Estadual Paulista Júlio de Mesquita Filho, Araraquara - SP - Brazil; 2Universidade de São Paulo, Ribeirão Preto - SP - Brazil. 1

Introduction and objective: Capillary electrophoresis (CE) is an environmentally friendly technique that generates low volumes of waste and low toxicity to operators and the environment. This technique fits perfectly with the principles of Green Chemistry, which promotes the use of knowledge and skills to reduce or eliminate the use and/or generation of toxic substances during the production and application of chemicals, in order to minimize damage to the health of operators and the environment. Thus, the aim of this work was the development and validation of a stability-indicating CE method for the analysis of daptomycin in powder for injectable solution, an important antimicrobial belonging to the class of cyclic lipopeptides, active against resistant Gram-positive bacteria. Materials and methods: The method was developed using an uncoated fused silica capillary with an internal diameter of 50 μm and a total length of 21 cm, and 15 mM potassium phosphate buffer at pH 7.0 as background electrolyte. Validation was carried out according to ICH guidelines. Daptomycin was subjected to forced degradation (acidic, alkaline, aqueous, photolytic and oxidative conditions) to prove the stability-capability of the method. Results and conclusion: Validation was successfully carried out and fulfilled all the recommended requirements. The method was shown to be adequate to quantify daptomycin in the pharmaceutical dosage form, in addition to being fast, stability-indicating, economical, safe for operators and environment. The application of this green technique in Quality Control should be increasingly encouraged. Financial support or fellowship: FAPESP (2013/02349-0); CNPq; PADC-FCF. CFSP225 CENTESIMAL COMPOSITION OF MINIMALLY PROCESSED AND INDUSTRIALIZED AÇAÍ BERRY PULP BRUNA TRINDADE RIBEIRO DE ARAUJO; ISABELA MUCHON PERRELLA; RAFAELA MENDES LOMBA PINHO; ELIZABETE LOURENÇO DA COSTA. Unisantos, Santos - SP - Brazil. Introduction and objective: The Açaí berry is one of the main products exported in Brazil and has great importance not only by being part of the food habit of the Amazon region, but also for its nutritional value. The increase in its consumption has been stimulated by disseminating its health benefits, associated with its chemical composition and antioxidant capacity. Objective: To analyze the centesimal composition of minimally processed açaí pulp without additives and compare it with the composition of industrialized açaí pulp. Materials and methods: Samples of the açaí pulp (industrialized and minimally processed) were obtained in the retail market of Santos. For determining the centesimal composition, the samples were submitted to cold extraction of the lipid fraction using the Bligh and Dyer (1959) method and the crude protein was determined by the method of Kjeldahl. Humidity was determined by dry kiln drying and the ash content by incineration in muffle at 550ºC. The carbohydrate content was obtained by the difference in the average of the other determinations. Results and conclusion: The minimally processed açaí berry showed 1.24% of proteins, 1.27% of lipids, 94.13% humidity, 0.31% ash content and 3.03% of carbohydrates. Compared with the composition of the industrialized açaí pulp, the protein content was the only nutrient that presented similar magnitude. The total lipids in the industrialized product were almost double, in the order of 2.17%. The humidity was 84.81% and the ash content obtained was 0.16%. The result of the theoretical calculation for the total carbohydrates was 11.62%. From the results obtained for the moisture content, it is concluded that minimally processed pulp is outside the standard of identity and quality established by the current legislation. When comparing the two products, it is possible to observe that the industrialized açaí pulp has increased concentration of total fat and sugars, and low mineral content, related to the use of additives, such as thickeners, emulsifiers and sugars. Financial support or fellowship: UNISANTOS

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CFSP226 CHARACTERIZATION BY NEAR-INFRARED SPECTROSCOPY OF XANTANA GUM PRODUCED USING CHEESE WHEY AS A FERMENTATION SUBSTRATE MARIA LAURA GAZOLLA PARMA1; ANA FLAVIA LAWALL WERNECK2; LOUISE CRISTINE CÂNDIDO DA SILVA2; MARIANA DA COSTA NOVO PIMENTA BRANDÃO3; MARIA JOSÉ VALENZUELA BELL2; HUMBERTO MOREIRA HÚNGARO2; MIRIAN PEREIRA RODARTE2; MIRIAM APARECIDA DE OLIVEIRA PINTO2. Universidade Federal de Juiz de Fora, Juiz de Fora - Brazil; 2Universidade Federal de Juiz de Fora, Juiz de Fora - MG - Brazil; Universidade Federal de Viçosa, Viçosa - MG - Brazil.

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Introduction and objective: Whey resulting from cheese production is often disposed of in the environment as waste. However, it stands out as a substrate that can be used in various biotechnological processes, such as the production of xanthan gum, due to its lactose, protein and salt content. Xanthan gum is a bacterial biopolymer synthesized by bacteria of the genus Xanthomonas. Due to its rheological properties, it has been one of the most widely used in the food and pharmaceutical sector. The objective of this research was to characterize, by infrared spectroscopy (NIR), xanthan gum synthesized by strains of Xanthomonas campestris using sterilized whey as the substrate Materials and methods: The strains of X. campestris pv. manihotis 1182, pv. campestris 1866, pv. mangiferaindicae 1230 and pv. pruni 2414 were applied in the fermentation process using cheese whey as substrate. The samples were analyzed by NIR Results and conclusion: NIR spectra of the experimental samples presented similar results, although they showed differences from the standard sample. Differences were observed in the absorption peaks in the C-H and N-H bonds regions. The absorption peaks corresponding to protein residues in their composition. These differences indicate that the variables of the bioprocesses used interfere in the composition of xanthan gum and suggests the necessity of a purification step of the experimental samples. The study generated useful knowledge applicable to the development and improvement of methods to obtain and characterize xanthan gum from cheese whey as a fermentation substrate. Financial support or fellowship: Financial support of “Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) “

CHARACTERIZATION OF ANACARDIC ACID LOADED-ZEIN NANOPARTICLES

CFSP227

JENNIFER THAYANNE CAVALCANTE DE ARAUJO1; FRANCISCO JAVIER OTERO ESPINAR2; FRANCISCO FABIO OLIVEIRA DE SOUSA1. Universidade Federal do Amapa, Macapa - AP - Brazil; 2University of Santiago de Compostela, Santiago de Compostela - Spain.

Introduction and objective: Cashew nutshell liquid (Anacardium occidentale) is rich in anacardic acid, reaching up to 70% of its composition. Anacardic acid has a variety of biological properties, including antioxidant, larvicidal, anti-inflammatory and antimicrobial activities. In view of these biological properties together with the nanotechnology concept, it was aimed to improve the use of this bioactive by incorporating zein-based polymer nanoparticles in order to improve its performance, stability and minimize the side effects by reducing the dose employed. Materials and methods: The nanoparticles were prepared by ionic solvation, solubilizing zein protein in hydroethanolic solution and adding the anacardic acid together with a dispersing agent (NanoZA). Control nanoparticles were also prepared, containing the dispersing agent (NanoZD) and the polymer (NanoZE) only. All nanoparticles were appreciated by transmission electron microscopy (TEM) and characterized in terms of particle size, polydispersity and zeta potential at predetermined times (0, 1, 7, 30 and 90 days) submitted to two storage conditions (room temperature, 20-25ºC) and (refrigerator, 3-8ºC). Results and conclusion: NanoZA remained more stable when stored in a refrigerator, size changed slightly from 381.6 to 378.0 nm and zeta potential from -15.9 mV to -13.5 mV, compared to room temperature, where those characteristics changed to 323.8 nm and -20 mV. The control nanoparticles were unstable; resulting in a drastic size increment and zeta potential inversion. In the TEM analysis it was possible to verify the surface coating of NanoZA, and the size consistent with the dynamic light scattering results. The incorporation of anacardic acid promoted physical-chemical stability to the nanoparticles, and it was also demonstrated that the best storage condition was in the refrigerator at 3-8ºC. Financial support or fellowship: PIBIC-CNPq

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CFSP228 CHARACTERIZATION OF ANTI-INFLAMMATORY PERFORMANCE OF TRANS-CHALCONE MICROCAPSULES FOR TREATMENT OF ULCERATIVE COLITIS IGOR REBECH1; LAISA GIORDANI VICENTE2; CARLA FABIANA SOUZA GUAZELLI2; ISABELA SALOMÃO LUDWIG2; RUBIA CASAGRANDE2; SANDRA REGINA GEORGETTI2; WALDICEU APARECIDO VERRI JUNIOR2; MARCELA MARIA BARACAT2. Universidade Estadual de Londrina, Londrina - Brazil; 2Universidade Estadual de Londrina, Londrina - PR - Brazil.

Introduction and objective: Inflammatory bowel diseases are frequency in the population. Ulcerative colitis is an idiopathic disease, chronic inflammatory disorder affecting the colon. The treatments include 5-aminosalicylic acid drugs, steroids, and immunosuppressants. Trans chalcona (T) is a flavonoid that in previous studies has been shown to down-regulate NF-kB- and STAT3-dependent signaling pathways. To increase the permeability of trans-chalcone, polymeric microcapsules of different drug polymer ratios were developed. Objectives: Characterization of the anti-inflammatory effect of trans-chalcone microcapsules (MT) and trans-chalcone non-microencapsulated on acetic acid-induced colitis in mice. Materials and methods: Two microencapsulated formulations were prepared (MT 1:3 and MT 1:5) by complex coacervation technique containing pectin/casein polymer loaded with T and inert microcapsules (IM). The anti-inflammatory capacity was measured by myeloperoxidase activity assay, levels of cytokine (TNF-α, IL-1β, IL-6 and IL-33) and NF-κB activity. Results and conclusion: The treatment with non-encapsulated T at the same dose of MT1:3 (0.3 mg/kg) was not sufficient to provide the beneficial effects. However, a MT 1:3 formulation with T significantly reduced neutrophil recruitment to the colon of mice after acetic acid-induced colitis, it also reduced pro-inflammatory cytokines levels, by reducing NF-kB activation of inflammation stress induced by acetic acid. It demonstrates the importance of pharmaceutical development of modified release systems to reduce the required dose of drugs and potentiate its effects. Financial support or fellowship: FUNDAÇÃO ARAUCÁRIA; CAPES CFSP229 CHARACTERIZATION OF CHITOSAN MEMBRANES REINFORCED BY NANOCRYSTALLINE CELLULOSE BRUNO VINCENZO FIOD RICCIO; TAYNARA BARBOSA DE SOUSA SZYMKO; EDUARDO PRESTES; PRISCILEILA COLERATO FERRARI. Universidade Estadual de Ponta Grossa, Ponta Grossa - PR - Brazil. Introduction and objective: Chitosan membranes (CM) have been studied for topical administration of drugs due to their biocompatibility, antimicrobial and adhesive properties. It was shown that the presence of Nanocrystalline cellulose (NCC) reinforcing fillers in the polymer matrix provides superior performances leading to the next generation of biodegradable materials. Objectives: to develop and to characterize CM reinforced by NCC containing betamethasone. Materials and methods: The CM-NCC were obtained by solvent casting using a 33-1 factorial design (samples M1 to M9) varying the concentration of NCC (2, 5, 8 %) betamethasone (drug; 0.025, 0.5, 0.1 %) and PEG 400 (porosity agent; 0, 1.0, 1.5 %). CM-NCCs were characterized by microscopy (SEM-FEG), by barrier (water vapor permeability (WVP) and swelling tests) and mechanical properties Results and conclusion: The values of WVP decreased with the increase in NCC content after 3 days, from 80.81 mg/cm2 for the pure CM down to 69.20 mg/cm2 for the 8% NCC. The presence of NCC significantly reduced the swelling percentage of CM (202.44 for CM down to 146.32 for M5). The tensile strength of pure CM was found to be 25 MPa and the CM-NCC increased the values up to 30, 35 and 39 MPa (2, 5, 8% of NCC respectively). CM-NCC due to their excellent mechanical and barrier properties should have a promising impact in topical delivery systems. Financial support or fellowship: FUNDAÇÃO ARAUCÁRIA”

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CFSP230 COMPARATIVE STUDY BETWEEN THE YIELD OF THE DRY GROSS EXTRACTS FROM THE LEAVES AND THE STEM OF THE CNIDOSCOLUS QUERCIFOLIUS POHL (FAVELA) ALANE ALEXANDRA DA SILVA OLIVEIRA1; IRTHYLLA NAYALLE DA SILVA MUNIZ2; JAMICELLY RAYANNA GOMES DA SILVA 2; YASMIM DAYANE LEAL PAIXÃO2; SARA BATISTA VASCONCELOS2; ARQUIMEDES FERNANDES MONTEIRO DE MELO2. Centro Universitário Tabosa de Almeida (Asces- Unita), Caruaru - PE - Brazil; 2Asces-Unita, Caruaru - PE - Brazil.

Introduction and objective: Substances extracted from medicinal plants are used progressively in the pharmaceutical, cosmetic and food industries. The Cnidoscolus quercifolius, known as Favela, contains abundant latex, usually applied against dermatoses, warts and tooth pains. There are also stories of the use of the plant for: healing, analgesic, anti-inflammatory, antibiotic and diuretic uses. This study aimed to analyze the yield of the Dry Gross Extract from the Favela, comparing the results of the leaves and the stem. Materials and methods: The stem and the leaves from this plant were collected and weighted, totaling 140.2g and 120.5g, respectively. These materials were crushed and then macerated for 7 days. The extrusion and evaporation of the extractive solution were performed until the loss of 95% of its humidity, and then were put in B.O.D. to obtain the dry gross extract, resulting in 0.07g (stem) and 2.95g (leaves). The yield of the extracts were calculated according to the formula: Yield= (Weight of the Dry Gross Extract/Weight from the fresh vegetable drug) x 100 and then expressed by percentage. Results and conclusion: Through this methodology the yield of the Dry Gross Extracts from the stem (%) = 0.04% and from the leaves (%) = 2.44% were estimated. Due to the fact of the biological effects being in the stem components, it becomes economically unfeasible for the Phytotherapy Industry. These data could be used as basis for the quality standard in future researches using this plant. Financial support or fellowship: ASCES-UNITA CFSP231 COMPARISON OF CENTRALISM COMPOSITION AND PHYCHOCHEMICAL SCREENING BETWEEN INDIA NUTS (ALEURITES MOLUCCANA) AND LUCKY NUTS (THEVETIA PERUVIANA). MAYRA IVONETE WESSLING; CARLA LANZA BELMONTE; LEONARDO LIMA FONTES DA SILVA; HIBA BACHIR; CAROLINA DE PAULA SOUZA JESUS. Unisantos, Santos - SP - Brazil. Introduction and objective: India nut (Aleurites moluccana) consists of a Candlenut seed which, according to popular culture, assists in weight loss, and few studies confirm its weight loss action. Its marketing is not regulated by Anvisa, leading buyers looking for fashionable, clandestine nuts often getting the fake nut in its place due to a morphological similarity among the seeds. The species marketed in place of the nut is Thevetia peruviana, popularly known as Lucky Nut, already known in literature as toxic. The active principles, present in the false nut accountable for its toxicity are cardioactive glycosides. The centesimal and phytochemical composition parameters can be used for botanical identification. The objective of this work was to compare these seeds, by analysing the centesimal and phytochemical composition. Materials and methods: Phytochemical screening was performed with colorimetric reactions and a determination of the centesimal composition was obtained per Kjeldahl method for proteins, Bligh and Dyer for lipids, and reactions for testing humidity and ashes. Results and conclusion: The main results found refer to the observation of the morphological correction between seeds, confirmation of the presence of cardioactive glycosides only in T. peruviana and the observation of the difference between the percentage of Lipids, Carbohydrates and Ash respectively 34.66%, 34.60% and 8.67% in the false nut and 62.20%, 18.50% and 2.01% in India Nut. With the obtained results it is concluded that centesimal composition and phytochemical screening are important parameters to identify falsification between similar-looking seeds. The morphological similarity among seeds reaffirms the need for more effective actions by enforcement agencies to restrict illegal trade, since the false nut presents high cardiotoxicity. Financial support or fellowship: PROGRAMA DE INICIAÇÃO CIENTÍFICA (PROITI)

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CFSP232 CYTOTOXICITY EVALUATION OF ETHANOLIC EXTRACT OF AZADIRACHTA INDICA (NEEM) LEAVES MAISA SAYURI NAGANO; ANDRÊSSA LUIZA OLIVEIRA ROCHA; CLAUDEMIR BATALINI. Universidade Federal de Mato Grosso – Cua - Lapquím – Unidade I, Pontal do Araguaia - MT - Brazil. Introduction and objective: The use of the Azadirachta indica plant has become important in herbal alternative therapy. The plant offers a constant source of new and unique phytochemical compounds used in the development of new drugs against many human diseases. The search for natural compounds with potential preventive and curative actions, without undesirable toxic effects has been growing in recent decades. Larvae of Artemia salina Leech have been used as bioassay for a variety of toxic substances and this method has also been applied in plant extracts to facilitate the isolation of biologically active compounds. The objective of this work is to evaluate the cytotoxic activity of the ethanolic extract from the leaves of the study plant in front of the larvae of Artemia salina Leech. Materials and methods: The ethanolic extract from leaves was tested for cytotoxicity at different concentrations (15 μg.mL -1, 50 μg.mL -1, 100 μg.mL -1, 500 μg.mL -1, 600 μg.mL -1 and 700 μg.mL-1) against the larvae of Artemia salina Leach. The cytotoxicity assay was an adaptation of the methodology of Meyer et al. (1982). In this test, a survival rate of the sample was obtained, and the damages caused to the larvae and the concentration that causes the death of 50% of them was observed, represented by the acronym LC50 (lethal concentration). The evaluation was performed in triplicate at each concentration tested and accompanied by a control sample for its veracity. Results and conclusion: The test for cytotoxicity indicated a very low degree of toxicity, where the LC50 test indicated low toxicity at concentrations up to 596,73 μg.mL-1 and demonstrated the confidence interval (95%) of 549.57 and 647.94 μg.mL-1. According to Meyer et al. (1982), lethal concentrations which are below 1000.0 μg.mL-1 indicate the potential presence of active substances. Thus, the concentration of 596.73 μg.mL-1 determines that the ethanolic extract of Azadirachta indica (neem) leaves contains substances that can confer medicinal properties.

CYTOTOXICITY EVALUATION OF TETRACAINE-LOADED NANOSTRUCTURED LIPID CARRIERS

CFSP233

SIMONE RAMOS DE CASTRO1; TALITA CESARIM MENDONÇA2; LIGIA NUNES DE MORAIS RIBEIRO1; GUSTAVO HENRIQUE RODRIGUES DA SILVA1; DAISY MACHADO1; ENEIDA DE PAULA1. Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas-Unicamp, Campinas - SP - Brazil; Universidade São Francisco, Campinas - SP - Brazil.

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Introduction and objective: Among the carriers used for drug-delivery, nanostructured lipid carriers (NLC) are very efficient for lipophilic compounds. NLC are composed of at least one solid lipid and one liquid lipid at room temperature, plus a surfactant stabilizer. Tetracaine (TTC) is a potent local anesthetic of the amino ester group, mainly used for topical anesthesia and spinal block. Its metabolism generates paraminobenzoic acid, the toxicity of which limits its therapeutic use. The aim of this study was to develop and investigate the effect of TTC-containing NLC on the cell viability of 3T3 fibroblasts in culture, as evaluated by reduction of MTT (β-dimethyl-2-thiazolyl-2-5-diphenyl-tetrazolium) or incorporation of the neutral red (NR) dye. Materials and methods: Tetracaine-loaded NLC formulations were prepared by the sonication-emulsification method, using different lipid combinations, such as: cetyl palmitate + DhayKol® (NLC-PC+DK) and myristyl myristate + DhayKol® (NLCMM+DK). The in vitro cytotoxicity was evaluated in 3T3 fibroblast cells, using MTTT and NR essays. Results and conclusion: TTC in solution showed IC50 values of ca. 0.6 mM, the toxicity being dose-dependent. 3T3 cells were also exposed to NLC-MM+DK and NLC-PC+DK formulations (without TTC), used as controls, which did not induce cytotoxicity at the tested concentrations (0.2 - 1.0 mM TTC). Finally, the cells were exposed to tetracaine-containing NLC, and the cytotoxic effect of the active in the formulations was lower than that of the free TTC for the NLC-MM+DK-TTC (IC50 = 0.8 mM) but nor for the NLC-PC+DK-TTC (IC50 = 0.6mM). These results indicate that NLC composed of MM+DK is the best formulation, since it has no intrinsic in vitro cytotoxicity and it was able to reduce the cytotoxicity of TTC. This formulation has proven safe and appropriate to be further evaluated in in vivo assays. Financial support or fellowship: CAPES; FAPESP (# 14/14457-5).

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CFSP234 DETERMINATION OF TOTAL TANNINS PRESENT IN LEAVES OF IPOMOEA PES CAPRAE OF THE COASTAL REGION OF THE STATE OF SÃO PAULO MARCO ANTONIO DE MOURA JUNIOR1; MARCELO JOSÉ DIAS SILVA2; WAGNER VILEGAS2; MILENE SAMPAIO CLEMENTE1. UNIP - Câmpus Santos Ragel, Santos - SP - Brazil; 2Instituto de Biociências - Câmpus do Litoral Paulista/Ibclp/Unesp, São Vicente - SP - Brazil. 1

Introduction and objective: Tannins are phenolic substances with great pharmacological potential. Their benefits go back centuries with wines and dermatological ointments being their latest examples of use. Ipomoea pes-caprae (Convolvulaceae) is a coastal plant with popular use against jellyfish burns. It has anti-inflammatory, antinociceptive, cicatrizing action among others (Dutra, 2013). The dosing and subsequent comparison of samples from several regions allows identification of the reasons that lead the plant to produce this singular metabolite. Objectives: Dose the tannins present in the leaves of the plant in different regions of the coast of the state of São Paulo and perform a comparison between the samples to elucidate the regions most conducive to the development of the plant rich in these metabolites. Materials and methods: The leaves were collected in June of 2017, in the morning, in four different cities of the coast of the state of São Paulo. The concentration of total tannins was determined by the protein precipitation method proposed by Hagerman and Butler (1978) and expressed as tannic acid equivalents. The entire process was performed in triplicate. Results and conclusion: The results obtained show a variation in tannin concentrations in the leaves of Ipomoea pes-caprae collected in the cities of Guarujá (166.872 ± 3.69 μg EAT g-1), Bertioga (139.164 ± 1.85 μg EAT g-1 ), Peruíbe (222.289 ± 7.39 μg EAT g-1) and Itanhaém (189.039 ± 0.01 μg EAT g-1) evidencing a great influence of environmental factors on plant metabolism, since in cities that have less pollution and preservation policies like Peruíbe city, the concentration of tannins was considerably higher. It is noteworthy that the collection period also has an influence on the amount of tannins, since in winter this concentration tends to decrease due to the scarcity of nutrients and reduced sunlight. The theme is important considering the need for studies that indicate the great influence of environmental and social factors on the production of secondary metabolites and the need to incorporate new standardized phytomedications in the pharmaceutical market. Financial support or fellowship: FAPESP, UNIP – SANTOS, UNESP – CLP CFSP235 DEVELOPMENT AND CHARACTERIZATION OF MOUTH DISSOLVING FILMS CONTAINING DOXASOZIN MESYLATE BY USING DESIGN OF EXPERIMENT ANNA CAROLLINA MORAES TAVARES; MARCELO DUTRA DUQUE; LETICIA NORMA CARPENTIERI RODRIGUES. Universidade Federal de São Paulo, Diadema - SP - Brazil. Introduction and objective: Researchers around the world are trying to exploit thin films as a new strategy for drug release. Among the major advantages of oral administration films over other pharmaceutical forms is their rapid dissolution rate, ease of transport and stability. Polymers are the major component of these formulations. An ideal film must have adequate flexibility, elasticity, and physical-chemical stability. Doxazosin is an alpha-1 adrenergic blocker used for treating high blood pressure and symptoms of benign prostatic hyperplasia. The objective of this project is to develop and characterize thin films for oral administration of doxasozine mesylate employing methylcellulose propylene glycol ether polymer. Materials and methods: The films were prepared according to factorial planning using Statistical software (Statsoft), using two factors and three levels, resulting in nine formulations. The factors evaluated were the amount of polymer and the nature of the plasticizer (glycerin, propylene glycol and polyethylene glycol 400. The films were prepared by casting method in oven drying with forced air circulation. Results and conclusion: Mouth dissolving time of the films were increased with increase in the concentrations of the polymer, as more fluid is required to wet the film in the mouth. Content uniformity study showed that the drug was uniformly distributed in the film. It can be concluded that mouth dissolving film containing doxasozin mesylate can be prepared by casting method. From the present investigation, it can be concluded that mouth dissolving film formulation can be a potential novel drug dosage form. Financial support or fellowship: The authors are grateful to Colorcom do Brasil for the gift sample of methylcellulose propylene glycol ether polymer and BrasEq for providing facilities to carry out this work.

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DEVELOPMENT AND CHARACTERIZATION OF O/A CHRYSIN MICROEMULSIONS

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CFSP236

ÍZOLA MORAIS DE MEDEIROS RAMALHO 1; BOLÍVAR PONCIANO GOULART DE LIMA DAMASCENO 2; LUCINDO JOSÉ QUINTANS JÚNIOR3; ÁDLEY ANTONINI NEVES DE LIMA4. Universidade Federal do Rio Grande do Norte, - Brazil; 2Universidade Estadual da Paraíba, Campina Grande - PB - Brazil; Universidade Federal do Sergipe, Aracaju - SE - Brazil; 4Universidade Federal do Rio Grande do Norte, Natal - RN - Brazil.

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Introduction and objective: Chrysin (5,7-Dihydroxyflavone) is a flavonoid that belongs to the flavone class. It is found naturally in honey, propolis and various other plant species. Like other flavonoids, chrysin (CS) also exhibits several biological activities. For example, antitumor, antioxidant, antinociceptive, anti-inflammatory activity, etc. It is already used in the US as a dietary supplement, however, its pharmaceutical application has been limited due to its low aqueous solubility and oral bioavailability. The aim of the study is to load chrysin in a microemulsion (ME) to optimize its bioavailability and therapeutic efficacy, allowing its pharmaceutical use. Materials and methods: The ternaty phases diagram (TPD) was constructed using LAS as surfactant isopropyl myristate (IPM) and osmosed water by titration method. The oil phase (IPM) and surfactant (LAS) were mixed in the proportions going from 1:9 to 9:1, followed by the addition of osmosed water by titration and sonication. Thereafter, those were characterized by visualization. Polarized light electron microscope and Abbé refractometer were used to confirm the isotropic system. Droplet size, polydispersity index (PI) and zeta potential (ZP) were analyzed by dynamic light scattering (DLS). The pH and electric conductivity were performed by direct immersion of the electrodes. Results and conclusion: The amount 1 mg.mL-1 CS was incorporated into a selected ME point from the TPD: MECS (5%IPM, 55% osmosed water, 40% LAS). The droplet size IP were 170 ± 5 nm and 0.1. The measured zeta potential was – 16mV. The pH was 4.5 ± 0.1. The conductivity was: 25 ± 0.2 µS cm-1. The constant refraction index of 1,3893 and the image from polarized light electron microscope showed we have an isotropic system. Therefore, the MECS was considered optimal to carry CS. Further studies of activity and release of MECS need to be continued to confirm the promising increase in the bioavailability and therapeutic efficacy of CS. Financial support or fellowship: CAPES; UFRN CFSP237 DEVELOPMENT AND QUALITY CONTROL OF CAPSULES CONTAINING DRY EXTRACT FROM THE LEAVES OF TURNERA SUBULATA Sm. WITH POTENTIAL GASTROPROTECTOR EFFECT FRANCISCO HUGO LEITE DE OLIVEIRA ARNAUD; CAMILA DE LIMA SILVA; RENAN PEREIRA DE LIMA; BEATRIZ MARIA DA SILVA BORGES; CARLOS RONNYE DA SILVA EVANGELISTA; MARIA ANGELINA DA SILVA MEDEIROS; ANGELO RONCALLI ALVES E SILVA. Universidade de Fortaleza - Unifor, Fortaleza - CE - Brazil. Introduction and objective: The Turnera subulata Sm, popularly known as chanana, is a plant of the Turneraceae family, which has reported several therapeutic properties, among them, antiulcerogenic. Several pharmaceutical forms can be used in phytotherapy, among them the capsule, presenting several advantages, such as ability to mask organoleptic characteristics, precise dosage per unit and fewer manufacturing steps. The objective of present study was to develop a capsule containing the dry extract of Turnera subulata Sm and quality control. Materials and methods: Capsules were developed containing the dry extract of Turnera subulata and the following tests were performed: disintegration and determination of the average weight, phytochemical tests to analyze the presence of secondary metabolites from a 70% hydroalcaholic extract. Results and conclusion: From the values obtained for the individual weights and the calculus of the average weight, the limit of variation was determined. The results obtained of the powder weight in the capsules ranged from 0.306g to 0.340g, with average weight of 0.323g, with a DPR of 2.9%, thus, within acceptable range limits of up to 7.5%. The disintegration test revealed a time of less than 45 minutes, within the specifications. By characterizing the secondary constituents, it is possible to confirm the presence of polyphenol compounds in the hydroalcoholic extract, such as flavonoids with predominance of catechins. Based on the above, the capsules are within the specifications.

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CFSP238 DEVELOPMENT AND VALIDATION OF A GREEN RP-HPLC METHOD FOR THE DETERMINATION AND QUANTIFICATION OF CEFADROXIL MONOHYDRATE IN CAPSULES BIANCA APARECIDA DE MARCO; HÉRIDA REGINA NUNES SALGADO. Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista- Unesp, Araraquara - SP - Brazil. Introduction and objective: Cefadroxil is a β-lactam antibiotic belonging to the first-generation cephalosporin group. It presents bactericidal activity being widely used in the treatment of infections caused mainly by Gram-positive bacteria. The sales of this antimicrobial are common and the studies related to it become inevitable, mainly on the quality control, which will guarantee the characteristics of the medicine and safety of the patients. The objective of this study was to develop and validate a reversed-phase high performance liquid chromatography (RP-HPLC) method for the determination and quantification of cefadroxil monohydrate and degradation products in pharmaceutical products, aiming to contribute to the green chemistry since it has low use of organic solvent and low production of toxic waste. Materials and methods: The RP-HPLC method was performed on a Waters Chromatography System e2695, connected to a Waters 2998 PDA detector. The separation was performed through isocratic conditions of reverse phase on an Agilent Zorbax-SB C18 column (150 x 4.6 mm; 5μm) maintained at room temperature. The mobile phase consisted of water + 0.1% orthophosphoric acid and ethanol (92:8), at a flow rate of 0.8 mL min-1 with 10 μL of the injection volumes, the wavelength chosen was 230 nm using UV/DAD detection. Results and conclusion: The validation parameters were in accordance with ICH guidelines and the method was shown to be linear, precise, robust and accurate over a concentration range of 20 to 120 μg mL-1 and was selective and stability-indicating due to the decrease of peak cefadroxil, and absence of significant additional peaks that could interfere with quantification of the molecule. The content of cefadroxil in the samples analyzed, 100.05%, is consistent with the official compendium from 90.0 to 120.0%. Financial support or fellowship: CAPES; FAPESP; PADC-FCF; EMS – Pharmaceutical industry CFSP239 DEVELOPMENT AND VALIDATION OF AN HPLC AND A UV SPECTROPHOTOMETRIC METHOD FOR DETERMINING ESOMEPRAZOLE IN PHARMACEUTICAL PREPARATIONS JESSICA VERTUAN RUFINO1; TIAGO BERVELIERI MADEIRA1; FERNANDO CESAR QUEIROZ DA SILVA1; FELIPE BRAGA TROMBETA1; MARIANA BORTHOLAZZI ALMEIDA1; MARLENE MARIA FREGONEZY NERY2; SUZANA LUXY NIXDORF1. Universidade Estadual de Londrina - Uel, Londrina - PR - Brazil; 2Universidade Estadual de Londrina - Uel, - .

Introduction and objective: Esomeprazole magnesium trihydrate, chemically described as bis (5-methoxy-2-[(S)-[(4- methoxy3,5-dimethyl-2-pyridinyl)methyl] sulfinyl]-H-benzimidazol-1-yl) magnesium trihydrate is an S-isomer of omeprazole, a proton pump inhibitor. Esomeprazole is used to treat peptic ulcer and gastroesophageal reflux disease. A high-performance liquid chromatographic (HPLC) and an ultraviolet (UV) method were developed and validated for quantitative determination of esomeprazole in pharmaceutical dosage form. Materials and methods: HPLC method is carried out by using a mobile phase acetonitrile: water acidified (40: 60, v/v, pH 3.5), flow rate of 1.0 mL/min, X-TERRA® C18 column (4.6 x 250 mm, 5 µm) with the drug presenting retention time of 4.3 minutes with a photodiode array detection at 305 nm. UV spectrophotometric method has been developed using NaOH 0.1 mol L-1.and λmax of 300.9 nm. The parameters studied were linearity, precision, accuracy, robustness, limit of quantification and detection, according to RE 899/03. Results and conclusion: For HPLC method analytical curves obtained by absorbance versus concentration, linearity in range of 5.00 to 100.00 µg mL-1 (R2> 0.99) was shown. This method was precise with 2.51 % of relative standard deviation and accurate presenting an average recovery of 99.86% in 3 levels of concentration. For UV spectrophotometric method linearity was established from 6.00 to 20.00 µg mL-1 (r > 0.99). Accuracy in three concentration levels was of 99.24% with limit of detection of 1.78 µg mL-1 and of quantitation of 5.93 µg mL-1. No tablet excipients interfere in UV spectra and in HPLC chromatogram. Both methods were successfully applied and can be used for routine analysis of raw material and in quality control of commercial esomeprazol tablets.

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CFSP240 DEVELOPMENT AND VALIDATION OF AN ANALYTIC METHOD FOR DOSING CHRYSIN IN MICROEMULSIONS SYSTEMS ÍZOLA MORAIS DE MEDEIROS RAMALHO 1; BOLÍVAR PONCIANO GOULART DE LIMA DAMASCENO 2; LUCINDO JOSÉ QUINTANS JÚNIOR3; ÁDLEY ANTONINI NEVES DE LIMA4. Universidade Federal do Rio Grande do Norte, - Brazil; 2Universidade Estadual da Paraíba, Campina Grande - PB - Brazil; Universidade Federal de Sergipe, Aracaju - SE - Brazil; 4Universidade Federal do Rio Grande do Norte, Natal - RN - Brazil.

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Introduction and objective: Chrysin (5,7-Dihydroxyflavone) is a flavonoid that belongs to the flavone class. It is found naturally in honey, propolis, and various species of plants. Like other flavonoids, chrysin (CS) also exhibits several biological activities (for example, antitumor, antioxidant, antinociceptive, anti-inflammatory activity, etc.) that have been explored by pharmaceutical researchers. It is already used in the US as a dietary supplement. Microemulsions (ME) are systems used to improve drug solubilization, therapeutic efficacy and drug delivery, and it is being studied as a promising system to deliver CS for pharmaceutical use. In this regard, the aim is to develop and validate a fast, effective, and cheap method for dosing chrysin in ME systems. Materials and methods: The method was validated according to resolution 899/2003 of the National Health Surveillance Agency (ANVISA). The parameters of selectivity, linearity and interval, precision, accuracy and robustness were evaluated. For CS dosing in ME, triplicate dilutions of the ME (4 μg.mL-1) were performed using ethanol as solvent. For the encapsulation efficiency, 1g of ME was subjected to centrifugation at 15000 g for 30 minutes and, then supernatant aliquots were extracted and diluted in the same conditions proposed before, followed by the dosage using the developed method. Results and conclusion: The analytical method was selective, showing linearity in the range of 1-8 μg.mL-1 with correlation coefficient (r) of 0.9999, limits of detection and quantification of 0.15 and 0.48 μg.mL-1, respectively. It also achieved the parameters of precision, accuracy and robustness. The dosed amount and the encapsulation efficiency of the ME were 4.21 μg.mL-1 and 97.7%, respectively. Thereby, the developed method met the parameters required by ANVISA, and can be used efficiently to dose CS in microemulsion systems. Financial support or fellowship: CAPES; UFRN CFSP241 DEVELOPMENT AND VALIDATION OF THE QUANTITATIVE ANALYSIS OF CEFADROXIL MONOHYDRATE IN CAPSULES BY FOURIER-TRANSFORM INFRARED SPECTROSCOPY (FT-IR) BIANCA APARECIDA DE MARCO; HÉRIDA REGINA NUNES SALGADO. Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista- Unesp, Araraquara - SP - Brazil. Introduction and objective: The transmission spectroscopy with Fourier-transform infrared (FT-IR), is a technique widely used by industries, research and areas of organic and inorganic chemistry because it is simple, reliable, easy to perform and safe for the determination and quantification of drugs. Investing in original and innovative analytical methods contributes to the environmental and economic impacts, favoring green chemistry directly to present social benefits with sustainable production and good management practices. Thus, the objective of this study was to propose an alternative method to quantify cefadroxil monohydrate capsules, in order to ensure favorable analyses to the environment and green chemistry, being able to be used as an alternative for routine analyses in quality control. Materials and methods: An FT-IR spectrometer IR- Prestige-21 model was used and the spectral region consisted of 4000 - 500 cm-1 at intervals of 2 cm-1. The band corresponding to group carbonyl in the molecule of cefadroxil, was selected to quantify the drug, using the support of IR Solution software, which consisted of the spectral region between 1800-1700 cm-1. Equivalent amounts between 1.5 and 2.5 mg of cefadroxil monohydrate were previously diluted in KBr (1:10 w/w) to obtain sufficient quantities of 150 mg of pellets of cefadroxil monohydrate. After adding the powders, the homogeneous mixture was submitted to mechanical pressing for 10 minutes, to prepare translucent pellets. Results and conclusion: The method was developed and validated according to the ICH and presented excellent results in parameters employed. The application of FT-IR spectroscopy can be used as an environmentally-friendly alternative for routine analysis in quality control for cefadroxil monohydrate and other drugs. Financial support or fellowship: CAPES; FAPESP; PADC-FCF; EMS – Pharmaceutical industry

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CFSP242 DEVELOPMENT OF A DISSOLUTION METHOD OF ARTEMETHER AND LUMEFANTRINE IN FIXED DOSE COMBINATION TABLETS: SIMULTANEOUS DETERMINATION BY HPLC-UV GRAZIELLA GOMES RIVELLI; GÉRSON ANTÔNIO PIANETTI; ISABELA DA COSTA CÉSAR. Faculdade de Farmácia, UFMG, Belo Horizonte - MG - Brazil. Introduction and objective: Artemether+lumefantrine (20 + 120 mg) has been proposed as a promising treatment for malaria as the main recommended association in endemic areas. Despite its widespread use, there is still scarce information about dissolution of artemether and lumefantrine, reflecting an absence of specific method in pharmacopoeias and international compendia, which becomes the purpose of this research. Materials and methods: Dissolution profiles were performed using twelve units of Coartem® tablets, at 900 mL of media, previously evaluated in equilibrium solubility tests: water, buffered solutions at pH 1.2, 4.5, 6.8 and 7.2, with and without surfactants. The HPLC analyses were carried out on a C18 column; mobile phase: acetonitrile and 0.5% triethylamine in water (80:20,v/v); flow rate: 1.2 mL/min and UV detection at 210 nm (artemether) and 390 nm (lumefantrine). Results and conclusion: Selectivity of the method was evaluated by spectral purity of artemether and lumefantrine chromatographic peaks. Linearity tests showed regression coefficients of 0.9995 and 0.9959 for artemether and lumefantrine, respectively. For inter-day precision, RSD values were 0.43% for artemether and 0.56% for lumefantrine. Recovery rates ranged from 100.65 to 101.33% for artemether and from 100.01 to 100.90% for lumefantrine. To evaluate the robustness, variation of media deaeration and sample filtration after dissolution test were analyzed and no significant difference between results was obtained. The best conditions in dissolution studies were obtained with media at pH 6.80 with 1% sodium lauryl sulfate for artemether and at pH 1.20 medium with 1% polysorbate 80 for lumefantrine, based on IVIVC studies. A simple dissolution method was successfully developed and completed validated for the evaluation of the in vitro release of artemether and lumefantrine in fixed combination tablets by HPLC. Financial support or fellowship: CNPq; MINISTRY OF HEALTH; CEDAFAR CFSP243 DEVELOPMENT OF A MUCOADHESIVE SYSTEM FOR CONTROLLED DELIVERY OF ANTIGLAUCOMATOUS SUBSTANCE LARISSA CALDEIRA NAVARRO; AINA LIZ ALVES CESAR; ANDRE AUGUSTO GOMES FARACO. UFMG, Belo Horizonte - MG - Brazil. Introduction and objective: Glaucoma is a worldwide problem and the second main cause of blindness in the world. The treatment consists of preserving the vision by slowing the damage to the optic nerve, through the reduction of intraocular pressure. The standard treatment consists of the administration of antiglaucomatous drugs in the pharmaceutical form of eye drops. This treatment, however, presents many problems due to low ocular bioavailability, unnecessary systemic exposure and low adherence, as multiple doses are necessary. An alternative is the use of ocular inserts, as a form of controlled delivered system, which guarantees the maintenance of an effective therapeutic concentration for a prolonged period. The purpose of this project was to develop systems with polymeric matrix consisting of chondroitin sulfate and chitosan then evaluate its physical characteristics. Materials and methods: The inserts were produced by solvent/casting method and were characterized through thermal analysis, infrared spectrometry, hydration potential, surface pH and in vitro sustained release profile. Results and conclusion: The characterization results suggested a strong interaction between the drug and the polymeric matrix. The inserts also present a sustained release profile, suggesting that this formulation has potential for in vivo studies Financial support or fellowship: Fapemig; CNPq; CAPES.

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CFSP244 DEVELOPMENT OF BAR SOAPS CONTAINNIG INNOVATIVE INGREDIENTS: COCOA LIQUOR AND SPRAYDRIED YOGURT POWDER MARIA ROSA BARBOSA FERREIRA1; KARINE GARGIONI PEREIRA CORREA DE MELLO1; EDISON PAULO DE ROS TRIBOLI2; GIOVANNA BONFANTE BORINI1. Universidade Nove de Julho, São Paulo - SP - Brazil; 2Centro Universitário Mauá de Tecnologia, São Caetano do Sul - SP - Brazil.

Introduction and objective: Bar soaps are hygiene and cleansing products used worldwide. They may be products intended only for hygiene and cosmetic use, and also intended for pharmaceutical purposes, depending on the type and concentration of active ingredients added to the soap base mass. Cocoa liquor is a mass extracted from cocoa beans (nibs) that contains both cocoa solids and cocoa butter. Its use in formulations is challenging, due to its high hydrophobic character, associated with its high solid content that can change the physical properties of formulations. Spray-dried yogurt powder is an innovative material, and its use in cosmetic products is a new field of research. The objective of this work was to prepare bar soaps containing spray-dried yogurt and cocoa liquor, separately, and evaluate the properties of formulations obtained compared to products available on the market. Materials and methods: Three formulations were selected for incorporation of each of the ingredients, in a total of six products, using two types of glycerin based soap mass. Cocoa liquor and yogurt powder were incorporated in two concentrations (0,5 and 1% w/w). The soaps were prepared by molding method and were evaluated for their organoleptic characteristics, weight-average, weight loss by storage, water absorption, loss of mass and softening, formation of cracks, formation of foam and pH value. Four commercial brands of bar soaps were also subjected to the same tests. Results and conclusion: The results obtained showed that the formulations are comparable to commercial soaps and that the incorporation of cocoa liquor and yogurt powder ingredients can be carried out in this type of system without compromising the evaluated properties and adding interesting cosmetic properties to this type of product. Financial support or fellowship: Universidade Nove de Julho CFSP245 DEVELOPMENT OF COSMETIC EMULSIONS CONTAINING MINERAL CLAYS USING DIFFERENT PROCESSES AND EVALUATION OF IN VITRO PHOTOPROTECTION JEMIMA DANIELA DIAS MORAES1; LUCAS OFFENBECKER GUERRA2; JULIANA CRISTINA DE MORAES3; BARBARAH HELENA NABARRETTI4; EDNA FERNANDES5; GISLAINE RICCI LEONARDI6. Departamento de Medicina-Programa de Pós-Graduação Em Medicina Translacional, Universidade Federal D, São Paulo - SP - Brazil; 2Lasa Pesquisas Laboratoriais/Programa de Pós Graduação Em Clínica Medica – Unicamp, Campinas - SP - Brazil; 3 Lasa Pesquisas Laboratoriais, Campinas - SP - Brazil; 4Programa de Pós Graduação Em Clínica Medica – Unicamp, Campinas - SP - Brazil; 5Programa de Pós Graduação Em Clínica Medica, Campinas - SP - Brazil; 6Faculdade de Ciências Farmacêuticas, Universidade Estadual de Campinas – Unicamp, Campinas - SP - Brazil. 1

Introduction and objective: Cosmetic emulsions containing clay minerals that provide skin protection against UVA and UVB solar radiation have been a trend. The homogenizer was used as part of the process in one batch because high pressure homogenization uses mechanical forces instead of heat which preserves sensitive ingredients that are positive for cosmetic formulations. The homogenization process can influence rheological behavior, sensorial and stability of emulsions positively. Objective: The development of a cosmetic emulsion containing clay minerals using different processes; without and with high pressure at 500 Bar in a PANDA PLUS 2000 homogenizer and also investigation of the UV protection by in vitro technique. Materials and methods: A cosmetic formulation was developed and packaged in two different batches (named with and without homogenization). Only the first batch was submitted to the homogenization at the end of emulsion development process. The two batches were evaluated by in vitro spectrophotometric, using LABSPHERE UV2000S technique. Results and conclusion: Both batches showed a maximum absorbance of 0.7ABS in the UVB range and 0.7ABS in the UVA range. They also presented integrated absorbance values of 19.3ABS-UVB range and 26.5 ABS-UVA range for the batch with homogenization and 19.1 ABS-UVB range and 27.6 ABS-UVA range for the batch without homogenization. Conclusion: The two batches offered the same in vitro FPS protection (value = 2.2). So, the use of different process did not modify the FPS value. Financial support or fellowship: CAPES

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CFSP246 DEVELOPMENT OF MICROSTRUCTURED SYSTEMS EMPLOYING SOLID DISPERSIONS CONTAINING THYME ESSENTIAL OIL WITH ANTI-PARASITAL PURPOSE BEATRIZ RODRIGUES SIVIERO1; BRUNO BATISTA DA SILVA2; MARCELO GUIMARÃES2. Universidade Presbiteriana Mackenzie, São Paulo - Brazil; 2Universidade Presbiteriana Mackenzie, São Paulo - SP - Brazil.

Introduction and objective: Nowadays, solid dispersions are an important field of research for the development of a drug delivery system that has hydrophobia as one of the most important characteristics, which hinders its bioavailability, causing new research to be discarded. The advantages provided are increased water solubility through the hydrophilic carrier dispersion and the micrometric decrease of the particles. Combined with the improvement of innovative drug systems, this research is an alternative in the use of a new antiparasitic drug, a field that urgently needs improvement due to the presence of resistance to the usual pharmacotherapy and the possibility of serious side effects. Therefore, the objective of this work is the development of solid dispersions using the essential oil of Thyme (Thymus vulgaris), known for its antiparasitic activity, being considered a new system of drug release. Materials and methods: The chosen preparation method was the melt-solvent technique, using polyethylene glycol (PEG) as the drug carrier. Its characterization took place through infrared spectrophotometry, differential thermal analysis and thermogravimetry, X-ray diffraction and electron microscopy tests. Results and conclusion: Electron microscopy analysis showed a decrease in particle size, in addition to the increase in the number of these particles. The comparative analyzes of infrared and X-ray diffraction suggest the correct encapsulation of the oil. In addition, comparative thermal analysis demonstrated a positive interaction between the drug and the carrier. Financial support or fellowship: PIBIC Mackenzie.

DEVELOPMENT OF MULTIPLE PHOTOPROTETIC EMULSION CONTAINING Pachira retusa OIL

CFSP247

DOUGLAS DOURADO1; NICOLE DE ALMEIDA NUNES MEIRA2; NEILA DE PAULA PEREIRA3; MYLA LÔBO1. Universidade Federal da Bahia, Salvador - BA - Brazil; 2Universidade Federal da Bahia, Salvador - Brazil; 3Universidade Federal da Bahia, Salador - BA - Brazil. 1

Introduction and objective: Sunscreens are formulated to reduce the sun’s impact on the skin. As the world temperature increases, it is necessary to optimize the formulations by inserting associated filters allowing a broad spectrum of protection. Objective: develop and characterize multiple photoprotective O/W/O emulsion, carrying anti UVA and anti UVB actives from the oil of Pachira retusa. Materials and methods: A primary O1/W emulsion containing vegetable oil, active octyl methoxycinnamate and Lanette® wax and a secondary W/O2 containing Shea butter, benzophenone-3 active, ethoxylated cetostearyl alcohol and glycerol monostearate both at 75 °C and constant stirring were formulated. These were reheated to 75 °C and by phase coupling, the W/O2 phase was poured into the O1/W under constant stirring, obtaining multiple systems. For the control formulation, vegetable oil is replaced with mineral. The emulsions were submitted to accelerated stability tests (ANVISA,2004). After preparation and post-accelerated aging, the scatterability profile was verified by the Knorts in vitro device. By spectrophotometry the presence of the active compounds in the formulations was investigated by means of scanning. Results and conclusion: The emulsions were stable, with pHs of 6.16 ± 032 and 5.94 ±0.28 for the emulsion containing the oil of P. retusa and the control respectively. Regarding the maximum spreadability, the emulsion with Pachira oil obtained a better performance: 3055.12/3301.47 mm2 (post preparation/aged) against the control of 2136.29/2245.13 mm2 (post preparation/aged). Such a fact can be justified by the presence of fatty acids majority of Pachira, palmitic (59%) and oleic (18%) emollients of high skin performance. The spectrophotometry confirmed the presence of the active compounds. In addition, this is the first study of multiple photoprotective emulsion, with optimized protection spectrum (anti UVA and UVB) containing the oil of P. retusa, adding value of raw material innovative and functional cosmetics. Financial support or fellowship: UFBA

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DEVELOPMENT OF POTASSIUM IODIDE-BASED SYRUP FOR SIMPLIFIED NOTIFICATION.

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CFSP248

THAIS DA SILVA REZENDE1; MIRTES IASMIM ALVES DE GOIS1; ANA CAROLINE NERES DOS SANTOS1; ROMILDO ALVES PAES BARRETO2; RAFAELA FERREIRAS DOS SANTOS MESTRE1; EVERTON RODRIGUES BEZERRA3; MARIA ROOSANY DOCARMO GOMES DE MOURA3. Faculdade de Integração do Sertão, Custodia - PE - Brazil; 2Industria de Medicamento Custodia, Custodia - PE - Brazil; Industria de Medicamentos Custodia, Custodia - PE - Brazil.

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Introduction and objective: Currently, the research and development of new chemical compounds brings a globalization to the pharmaceutical industry, with this the present study, together with the IMEC drug industry, aims to demonstrate the innovation of pharmaceuticals products in the R & D sector. Potassium iodide syrup, with a concentration of 20mg/ml, developed for therapeutic profusion is a concentrated aqueous pharmaceutical form of sucrose, which makes it resistant to contamination. Objectives: to plan, develop and achieve the best characterization of the product according to the official methodologies and compendia required by the regulatory institution - ANVISA. Materials and methods: Market research was conducted to identify the pharmaceutical form, therapeutic class and preformulation of the aforementioned drug. The formulation was developed through bench tests, so that all the excipients were adequate and integrated the formulation. The Handbook of Pharmaceutical Excipients was used as a research source to verify the pharmacotechnical characteristics of each raw material for the production. Among the pilot lots produced the more stable formulation was forwarded for accelerated stability and long duration stability to verify whether it would present non-conformity in its physical-chemical properties. Results and conclusion: A technical report was prepared describing the research carried out on all the raw materials, in order to produce a formulation that complied with the pharmacopoeial specifications. Seven pilot batches of potassium iodide were produced, of which only one obtained the desired formulation without any incompatibility between the excipients, thus meeting all the established parameters. Financial support or fellowship: IMEC, FIS CFSP249 DEVELOPMENT OF THERAPEUTIC ADHESIVES CONTAINING SUCUPIRA OIL-RESIN AS ANTIINFLAMMATORY AGENT JOÃO BATISTA DA SILVA FILHO; FLÁVIO MARQUES LOPES. Universidade Federal de Goiás, Goiânia - GO - Brazil. Introduction and objective: To solve the delivery mechanism of several drugs, a range of technological innovations has been developed, and therapeutic adhesives are an alternative. The Anadenanthera macrocarpa (Angico vermelho) is able to expel a milky exudate with gum properties, being the main raw material of the biofilm. In these prototypes, the active ingredients must be dissolved or dispersed within the matrices/hydrogels. The development of new low-cost biodegradable adhesives has been a goal of industries for its easy obtaining and good employability. This research aims to develop a therapeutic anti-inflammatory adhesive using xanthan, angico polysaccharide and sucupira oil-resin. Materials and methods: Two raised to three factorial design with center point, varying the concentration of glycerol, xanthan gum and angico polysaccharide (pegico), base gel composed by PVA 10% (pH 2.0), 0.125% of glutaraldehyde as crosslinking agent,

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followed by water evaporation in heated chamber for 24 hours. Sucupira oil nanocapsules using P407 and P188 as surfactants and PLGA as polymer were provided. The mechanical properties, such as deformation and resistance, were analyzed by a texturometer. Solubility were analyzed by leaving a small piece of adhesive, previously weighed, in 50 mL of water for 24 hours and a further 24 hours out of water, at room temperature. The loss of mass in water was evaluated. Results and conclusion: To obtain a control and establish the most promissory adhesives, formulations containing constant amounts of glutaraldehyde (80 µL) and PVA 10% (6 mL) were proposed. On the other hand, varying concentrations of 5 mL of xanthan solution (0.0%, 0.1%, 0.55% 1.0%, and 1.34%), 2 mL of pegico solution (0.0%, 0.5%, 1.25%, 2.0% and 2.57%) glycerol (0.0 µL, 100 µL, 250 µL, 400 µL and 500 µL) were applied, totalizing 16 films. The films were divided into three categories: 1) liquefied; 2) jelly-like; 3) film formed but did not detach naturally from the dish and 4) film formed and easily detached from the dish. Only two films fitted category 4: 7) xanthan 1.0%, pegico 0.5%, 400 µL of glycerol; 15) xanthan 0.55%, pegico 2.57% and 250 µL of glycerol. These two did not solidify after nanocapsule incorporation, showing a large amount of it or incompatibility. Solubility and resistance data obtained were satisfactory. The importance of glycerol and xanthan in film compositions highlights the enhance of adhesive’s biodegradable potential, as these two ingredients are soluble in water. Future analysis will be able to make clear if, after nanocapsule incorporation, the seven films in category 3 present the expected features. Financial support or fellowship: CAPES; CNPq. CFSP250 EFFECT OF DIFFERENT POTENCIES OF BELLADONNA IN CULTURE OF STREPTOCOCCUS PYOGENES “IN VITRO” MARCELLA EIRAS DA ROSSI; ANDREA DE ANDRADE RUGGIERO; TÂNIA AGUIAR PASSETI; LEANDRO RIBEIRO BISSOLI. Faculdade de Medicina do Abc, Santo André - SP - Brazil. Introduction and objective: Streptococcus pyogenes is a pathogen that causes pharyngitis, erysipelas, impetigo, and endocarditis, all of which are effectively treated with antibiotics. In some patients, a secondary reaction such as scarlet fever and rheumatic fever may occur. Hahnemann used Belladonna during the scarlet fever epidemic in Germany and obtained excellent results. We proposed to study the efficacy of different potencies of the homeopathic medicine Belladonna on the growth of S. pyogenes bacteria “in vitro”. Materials and methods: Belladonna was used in the dilutions of 1 to 30 CH, continuously, and alternating dilutions of 36, 42, 45, 48, 54, and 60 CH in liquid Mueller Hinton (MH) medium and diluted S. pyogenes bacteria. The suspension was incubated for 48 h at 37° C. Each sample was added to a 96-multiwell plate and read at 620 nm. The drug was also evaluated in a hemoglobin dosage test. The dynamizations described above were added in MH medium, 5% of sterile sheep red cells and diluted S. pyogenes bacteria. The suspension was incubated for 48 h at 37° C and then centrifuged at 2000 rpm for 5 min. The supernatant was added to a 96-multiwell plate and analyzed through a green filter reader. Results and conclusion: Belladonna was able to inhibit the growth of S. pyogenes at several potencies. The results showing statistically significant inhibition of bacterial growth are compatible with the main potencies suggested in some Materia Medica, particularly the 7CH, 15 CH and 30 CH potencies. Hemoglobin dosage test results demonstrated a significant increase of this substance in media with the bacterium incubated with high potencies of Belladonna. Due to the originality of this method, other tests are needed. These results demonstrate that different potencies of the Belladonna homeopathic medicine reduce the growth of S. pyogenes “in vitro” and possibly decrease the protein production of these bacterial cells. Financial support or fellowship: NEPAS (FMABC)

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CFSP251 EFFECT OF OZONE ON THE HISTOLOGICAL CHARACTERISTICS AND DISINFECTION OF BULLFROG SKINS (RANA CATESBEIANA) WITH THERAPEUTIC PURPOSES (HEALING MEMBRANES). DIONE NATUREZA DE MORAES; ADRIANA BARRINHA FERNANDES MORETTI; DORA INÊS KOZUSNYANDREANI; RICARDO SCARPARO NAVARRO. Pós-Graduação Engenharia Biomédica e Bioengenharia- Universidade Brasil, São Paulo - SP - Brazil. Introduction and objective: Currently, various biological materials are being studied as possible healing biological membranes on skin. The use of amphibians’ skins such as Bullfrog (Rana catesbeiana) because its biological properties, great source of collagen and oils studies show the promising use as healing membrane in burned skin lesions. Previous studies demonstrated the action of ozone in disinfection processes of different substrates and applications in fields of health and industry. This premise make the present study innovative and pioneering, because it evaluated the effect of ozonated water (O3) in disinfection of frog skin (Rana catesbeiana) used as therapeutic healing skin wounds membranes. Materials and methods: Standardized samples of frog skin (1cm2) were analyzed for microbial contamination and histological characteristics before and after different treatments: G1: sterile saline solution, G2-antibiotics (AB): penicillin, streptomycin, gentamicin; G3-O3/5 min; G4-O3/10 min; G5-O3/12 min; G6-O3/15 min; G7-O3/20 min. The values of microbiological log reduction were submitted to ANOVA and Tukey test. Results and conclusion: The ozonated water (O3) with different times promoted effective microbiological reduction (p< 0.001); AB and O3/20min promoted higher values of microbiological reduction (p0.001). The AB and ozone promoted no histological changes in frog skin. Ozone was an effective method for disinfection of the frog skin, enabling its future use as an alternative method to antibiotics, as treatment of healing biological membranes for clinical use as therapy in cutaneous wounds.

EFFECTS OF BURITI OIL (Mauritia Flexuosa L.) ON WOUND HEALING IN RATS

CFSP252

JULIANA MARIA MACHADO TURATI; ANDREA DE ANDRADE RUGGIERO; JULIANA MORA VERIDIANO; GIULIANA PETRI; OLGA MARIA DE TOLEDO CORREA; ROBSON MIRANDA DA GAMA; JOSÉ ARMANDO JÚNIOR. Faculdade de Medicina do Abc, Santo André - SP - Brazil. Introduction and objective: Vegetable oils are traditionally used for the treatment of wounds because of their high antioxidant activities, shown to be effective in tissue repairing. Buriti oil (Mauritia flexuosa L.) is rich in carotenoid and tocopherol associated to this physiological process. The aim of this work was to assess the excision wound healing activity of crude Buriti oil (BO) compared to mineral oil (MO) in Wistar Rattus norvegicus male. Materials and methods: Two parallel incisions (9.0 cm long x 2.0 cm wide) were made on the right and left sides of the rat’s back and the tissues were prepared for histological analysis, on day 3, 7 and 14 after the beginning of the assay. Results and conclusion: After 3 days, both areas of wound showed fibrinous crust and in their surroundings, new blood vessels and large amounts of inflammatory cells were noticed. The treated area of both groups showed no reepithelization signals tissue. Also, a decreased inflammatory infiltrate, lots of newly formed vessels and intense inflammatory infiltrates was observed. After 7 days the 2 treatments, the presence of granulation tissue and fibroblastic proliferation was observed in the lesion. In addition, the BO treated area showed thicker collagen fibers from the edges of the lesion towards its center. At the end of 14th day, both areas showed a reduction of blood vessels, fibroblasts and large amount of collagen fibers in the extracellular matrix of connective tissue (dermis), but, in the BO treated area, there was a better matrix fiber organization. The repairing process was faster when compared to the MO treated area. The use of crude oil suggests a marked improvement in the healing process, not only because of the injury closing, but also by the presence of formation of numerous new blood vessels, which characterizes the speed of the reparative process and, finally, the fiber arrangement.

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CFSP253 EFFECTS OF ETHYL ACETATE AND DICHLOROMETHANE FRACTIONS FROM MANDEVILLA MORICANDIANA LEAVES ON VASCULAR REACTIVITY IN ISOLATED AORTA FROM OBESE AND DIABETIC RATS CINTHYA MACIEL DE MELO1; LETICIA LIMA DIAS MOREIRA FERREIRA2; LEANDRO LOUBACK DA SILVA1; MICHELLE FRAZÃO MUZITANO1; IVANA CORREA LEAL3; JULIANA MONTANI RAIMUNDO1. Universidade Federal Do Rio De Janeiro - Campus Macaé, Rio De Janeiro, Rj, Brazil, Macae - Rj - Brazil; 2Universidade Federal Do Rio De Janeiro - Campus Macaé, Rio De Janeiro, Rj, Brazil; Universidade Fede, Macae - Rj - Brazil; 3Universidade Federal Do Rio De Janeiro, Rio De Janeiro, Rj, Brazil. 1

Introduction and objective: We have recently shown that the ethyl acetate (MMAc) and dichloromethane (MMDic) fractions of the hydroalcoholic extract of Mandevilla moricandiana leaves have vasodilatory and antioxidant activities. The aim of this work was to investigate the effects of MMAc and MMDic fractions on vascular reactivity of aorta from obese and diabetic rats. Materials and methods: For the obesity model, male Wistar rats received subcutaneous injections of monosodium glutamate (MSG; 4 g/kg body weight) during the first 5 days of life and experiments were performed at 150 days of age. For the diabetes model, male Wistar rats (140-180 g) received a single injection of streptozotocin (60 mg/kg body weight) and experiments were carried out after 11 weeks. Vascular reactivity was evaluated in aortic rings prepared for isometric tension recording. Concentrationresponse curves to phenylephrine and acetylcholine (10-9 - 10-5 M) were obtained in the absence and presence of 3 μg/mL of MMAc or MMDic fractions. The local Animal Care and Use Committee approved all protocols (License MAC019). Results and conclusion: Obese rats presented higher perigonadal and retroperitoneal fat stores compared to control rats. Diabetic rats showed sustained glycemia values above 250 mg/dL. In MSG aortas, maximum relaxation to acetylcholine was increased from 31.60 ± 1.85% to 63.34 ± 5.36% (P <0.05) in the presence of MMAc, and from 40.04 ± 4.50% to 64.97 ± 3.44% (P <0.05) in the presence of MMDic. However, both fractions had no effect on vascular reactivity in aorta from diabetic rats. Conclusion: MMAc and MMDic fractions improved vascular dysfunction only in the obesity model. Financial support or fellowship: CNPq-Pibic; CAPES; FAPERJ; CNPq. CFSP254 ELABORATION AND QUALITY CONTROL OF EXFOLIATING CREAM WITH ELDERBERRY FLOWERS NILSA SUMIE YAMASHITA WADT1; BRUNA MOTA CAMPOS2; FABIANA VALENTIM DA SILVA2; LÍVIA JULIANA GUIMARÃES CARNEIRO BORGES2; NEUSA REGINA DOS SANTOS SILVA2; SOFIA CAMPOS ALMEIDA2. UNIP, Valinhos - SP - Brazil; 2UNIP, Jundiai - SP - Brazil.

Introduction and objective: Plant drugs are being widely used in cosmetics, as well as their derivatives. The elderberry is a plant that possesses acids that may be used as chemical exfoliant and its flower as a physical exfoliant. Plants that contain tannins, such as red Brazilian berry and barbatiman, possess antioxidant, skin healing and antimicrobial activity. The objective of the present work was to elaborate quality control of exfoliating cream based on red Brazilian berry and barbatiman extract with elderberry leaves. Materials and methods: the extracts were prepared by percolation, and the cream used as base was non-ionic cream. Elderberry flowers were ground, sieved and sterilized by autoclaving and UV light. The quality control of antimicrobial activity for elderberry flowers, and also for the cream, was performed by agar depth method and pathogens research. Results and conclusion: the flowers sterilized by UV presented, for both fungus and aerobic bacteria, a total count of >1000 CFU/g, however those which were autoclaved did not show microbial growth. The cream did not present fungal growth, and for bacteria, the total count was 1 CFU/g. It is possible to conclude that the best sterilization method for elderberry flowers is autoclaving, and the cream elaborated with flowers and extract is within the legal limits, with use in humans allowed. Financial support or fellowship: UNIP

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CFSP255 ENANTIOMERIC PURITY EVALUATION OF NADOLOL USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH CHIRAL STATIONARY PHASE GABRIELA SOUTO CARDOSO; ANIL KUMAR SINGH. Universidade de São Paulo, São Paulo - SP - Brazil. Introduction and objective: Most antihypertensive drugs currently prescribed are marketed under the racemic form, despite the well-known fact that the presence of different enantiomers in a pharmaceutical formulation can lead to distinct pharmacological activities, dissimilar pharmacokinetic and toxicological profiles. The characterization and quantification of enantiomers in pharmaceutical drug substances and drug products became mandatory with the implementation of RDC n° 57, in 2009 by Brazilian drug regulatory agency (ANVISA). These regulatory changes lead to an increase in the demand for rapid, accurate and sensitive methods, for the determination of enantiomeric purity of chiral drug substances. The present research work aspires to develop innovative strategies for enantiomeric separation of nadolol in semipreparative scale and consequently make use of these pure enantiomers in enantioselective quality control. Materials and methods: For the enantiomeric separation, we used a chiral stationary phase (Chiralcel OD® semi preparative column). All separations were obtained in normal phase mode, with mobile phase composed of hexane, ethanol, diethylamine and glacial acetic acid. The system was prepared and the parameters were set to obtain optimum selectivity between adjacent peaks. Enantiomerically pure fractions were collected by peak shaving method and enantiomeric purity of each fraction was analyzed by injection into a Chiralcel OD® analytic column. Results and conclusion: The enantioselective properties of Chiralcel OD® may be affected by several chromatographic parameters, such as analyte mass and volume of injection, column backpressure and oven temperature. These parameters must be adjusted to obtain acceptable separation and resolution amongst antipodes. The enantiomers were successfully separated and the analytical column assays demonstrated a significantly pure fraction of nadolol. Financial support or fellowship: FAPESP CFSP256 ENDOCRINAL-METABOLIC EVALUATION OF GLYCINE MAX IN POLARISTIC OVARIAN MODEL INDUCED IN RATS. Anderson Assis da Silva1; Selma do Nascimento Silva1; Adriano Jardel da Cruz Ericeira1; Iracelle Carvalho Abreu2; Caio da Costa Cunha1; Fernanda Teixeira Silva1. 1 UFMA, São Luis - MA - Brazil; 2Ufma. Introduction and objective: Polycystic ovary syndrome (PCOS) is a neuro-endocrine-reproductive disorder that affects 5-10% of women of reproductive age. Insulin resistance and compensatory hyperinsulinemia are hallmarks of PCOS. As isoflavones found in Glycine max (L.), known as soy, hypoglycemic acceptance, in addition to chemical structure similar to endogenous estrogen. Investigate the effects of Glycine max on the lipid and glucose metabolism of experimental model of polycystic ovary syndrome (PCOS) induced in rats. Materials and methods: Rats were divided into two groups: Control (CTRL not induced, n = 6), Induced (SOP-IND, induced with testosterone 10mg/kg), subdivided into SOP-Saline (treatment with saline, 0.1ml/100g; N = 9); SOP-MET (treatment with metformin, 200 mg/kg, v.o., n = 5) and SOP-ISO (treatment with Glycine Max-Isoflavone extract at concentrations of 100 mg/ kg and 200 mg/kg). During the development of body weight, feed intake and determination of the ovarian cycle biochemical measurements of total proteins, albumin, urea, glucose, triglycerides, total cholesterol, HDL, ALT and AST were performed, and retroperitoneal adipose tissue was collected for histological and morphometric analyzes of adipocytes and mean abdominal fat weight. The results were submitted to Analysis of Variance for the Newman Keuls test at a significance level of 95% (p <0.05). Results and conclusion: Induction maintained the non-proliferative phases of the cycle, diestrus and metaestrus, with 60.57% and 39.43%, respectively. Glycine max supplementation promoted an increase in proliferative phases (ISO-100: proestro - 5.47% and estrus - 2.91%, ISO-200: proestro -2.49% and estrus - 2.13%;). The soy isoflavones extract (100 and 200mg/kg) was able to regress glucose levels at 96 ± 2.870mg/dL and 103.6 ± 1.131mg/dL, respectively, in relation to the induced group (114.6 ± 3.803 Mg/dL). The weight of adipose tissues, area and diameter of the adipocytes was smaller in the isoflavones groups, compared to the SOP-IND group. However, there was no difference in feed consumption and lipid profile among the experimental groups. The administration of Glycine max in rats with PCOS shows signs of phytoestrogenic effects at doses of 100mg/kg and 200mg/kg, improving metabolic syndrome related indexes such as body weight, abdominal fat and glycemia. Financial support or fellowship: UFMA; FAPEMA

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EVALUATION OF (-)-CUBEBIN IN INDUCING ERECTION IN MICE

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CFSP257

MARCIO LUIS ANBDRADE E SILVA1; NAYANNE LARISSA CUNHA1; SÉRGIO DE ALBUQUERQUE2; RODRIGO LUCARINI1; KÁTIA JORGE CIUFFI1; PAULO SÉRGIO CALEFI3; EDUARDO JOSÉ NASSAR1; ROSÂNGELA DA SILVA LAURENTZ4; ADEMAR ALVES DA SILVA FILHO5; WILSON ROBERTO CUNHA1; JAIRO KENUPP BASTOS2. Unifran, Franca - SP - Brazil; 2Fcfrp-USP, Ribeirão Preto - SP - Brazil; 3Ebtt – Ifsp, Sertaozinho - SP - Brazil; 4Feis-Unesp Ilha Solteira, Ilha Solteira - SP - Brazil; 5Ff-Uf-Juiz de Fora, Juiz de Fora - MG - Brazil. 1

Introduction and objective: (-)-cubebin (CUB), a dibenzylbutyrolactolic lignan, displays anti-inflammatory and antiparasitical activities. Erectile dysfunction (ED) is characterized by the persistent inability to obtain or maintain adequate penile stiffness. Objectives: to isolate (-)-cubebin from the dried seeds of Piper cubeba and its evaluation on erection activity. Materials and methods: (-)-Cubebin was isolated from P. cubeba seeds by chromatographic means. Four groups of six Swiss mice, each aged 18-24 weeks, were assayed for erectile dysfunction, as follows: negative control (saline), positive control (Tadalafil), CUB 20 mg i.p and CUB 40 mg orally. Animals of all groups were maintained under the same conditions for 4 h, after treatment for further evaluation of penile erection. The beginning latency and number of erections were observed. Afterwards, the animals were sacrificed in accordance with ethic guidelines Certificate Number: 005/09-AMB. Results and conclusion: In relation to the initiation of the erection, CUB 20 mg i.p and CUB 40 orally induced erection within 33 min and 45 min, respectively, after administration, which was faster than for the positive control group (50 min). With respect to the erection latency, CUB 20 mg, i.p., CUB 40 mg, orally, and positive control showed the same erection time (35 sec). Finally, by observing the number of erections for 30 min, both groups treated with CUB 20 mg ip and CUB 40 mg orally had more erections in 30 min (17) than the positive control (7). Financial support or fellowship: CNPq, CAPES, FAPESP, VILIFARM, NPA CFSP258 EVALUATION OF ALGINATE BIODRESSINGS CONTAINING SODIUM DICLOFENAC: ANTIMICROBIAL AND CYTOTOXICITY ACTIVITY LETÍCIA DE ALMEIDA PAULO1; MARIANA AGOSTINI DE MORAES2. Unifesp Diadema, Diadema - SP - Brazil; 2Unifesp, Diadema - SP - Brazil.

Introduction and objective: The research focused on the development of dressings is necessary as healing is a chronic and painful process. It is interesting to create films that, besides the healing effect, also have the curative effect when having a drug incorporated into them. The success of these films depends on procedures to ensure their physical, chemical and biological stability. The goal of the current study was to evaluate the antimicrobial activity of alginate films in addition to assessing their cytotoxicity. Materials and methods: Microbiological analyses were assessed in Petri dishes containing nutrient agar in bilayer, against Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 8539), Candida albicans (ATCC 10231) and Pseudomonas aeruginosa (ATCC 9027), evaluating alginate discs, alginate + diclofenac sodium and filter papers with the drug in the concentration contained in the films, in duplicate. After the plates were incubated for 24-48 h, measurement of the halos of the bactericidal or bacteriostatic action were assessed using the same nutrient agar and incubated for 24h. Cytotoxicity was evaluated by the method stated in ISO 10993-5, using CHO cells and viability was determined by Neutral Red Uptake method (NRU) at 540nm. Results and conclusion: The results showed that the dressings did not show antimicrobial activity. The films presented above 70% cell viability so they are considered safe. These results indicate that the film developed offers the potential to be used as dressing so the physical, physicochemical and biological characterization will be continued. Financial support or fellowship: FAPESP

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CFSP259 EVALUATION OF ANTIMICROBIAL ACTIVITY OF COMMERCIAL SAMPLES OF ESSENTIAL OILS ON BIOFILM FORMING BACTERIA ANNA BEATRIZ GOMES DA SILVA1; MARCOS AUGUSTO DE ASSIS BRASILEIRO1; JULIANO MORAIS FERREIRA DA SILVA2; MÔNICA VALERO DA SILVA1. Unb, Brasília - DF - Brazil; 2Cassi, Brasília - DF - Brazil.

Introduction and objective: Biofilms formed by microorganisms are a challenge in the pharmaceutical, cosmetic and food areas. The objective was to evaluate the antimicrobial activity of essential oils (EO) of tea tree, lemongrass and clove in different concentrations (6.25%, 12.5%, 25%, 50% and 75%) against Escherichia coli, Pseudomonas aeruginosa and Bacillus sp. with subsequent challenge against biofilms. Materials and methods: Evaluation of the in vitro antimicrobial activity of EO was performed using aseptic microplate dilution techniques. The test wells were filled with 150 μL of EO 50% (v/v) and 10 μL of the test microorganisms (10^8 CFU.mL-1), also employing positive and negative controls. The reading was taken in a spectrophotometer UV visible, wavelength of 620 nm, in the zero hour (T0) and after incubation of 24 hours at 35°C (T24). Minimal Inhibitory Concentration (MIC) determination tests were performed from the dilutions (50%, 25%, 12.5% and 6.25%) of the EO, with subsequent evaluation of the biofilm susceptibility to OE. Results and conclusion: The main result was the antimicrobial activity of melaleuca and lemongrass, which were found to inhibit microbial growth at rates of 96.28% and 98.64% (E. coli) and 93.52% and 98.61% % (Bacillus spp.), respectively, when suspended for MIC (6.25%). No inactivation results were observed for any essential oil against biofilms. Tests involving Pseudomonas aeruginosa showed no inactivation by any of the oils studied, clove showed excessive turbidity, thus preventing the reading. Variations in microbial inhibition of the same OE, but of different commercial brands were found, as well as absence of inhibition of the biofilms formed by the challenging strains. Further studies should be performed with other OE on the mentioned microorganisms. CFSP260 EVALUATION OF ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL OF OCIMUM GRATISSIMUM ON STAPHYLOCOCCUS AUREUS FOR FACIAL CLEANING COSMETIC DEVELOPMENT MARIA JOSIENE FARIAS CRUZ1; NAIRA LIMA SILVA1; JESSICA LIMA CAETANO1; ANDRÉA BESSA TEIXEIRA2; SUZANA BARBOSA BEZERRA2. Undergraduate Student in Pharmacy at Faculdade Metropolitana da Grande Fortaleza - Fametro, Fortaleza - CE - Brazil; Professor of Faculty of Pharmacy at Faculdade Metropolitana da Grande Fortaleza - Fametro, Fortaleza - CE - Brazil.

1 2

Introduction and objective: Ocimum gratissimum plant, belonging to the Labiatae family, is popularly known as alfavaca-cravo, recognized by its fragrance reminiscent of clove. This plant species has some recognized biological activities, such as insecticidal and bactericidal, mainly related to the presence of the essential oil in the plant. The presence of thymol, cimen-8-ol and transcaryophyllene compounds, and mainly eugenol (major compound), are responsible for the properties of the species. The aim of the present study was to evaluate the antimicrobial potential of alfavaca-cravo essential oil (ACEO), which will be used as an active ingredient in the development of a cosmetic facial cleansing formulation. Materials and methods: The antimicrobial potential of ACEO extracted from the fresh leaves of Ocimum gratissimum by hydrodistillation was tested on the strain Staphylococcus aureus ATCC 6538P using the modified agar diffusion methodology. Microbial suspensions with approximately 108 CFU/mL (visible turbidity adjusted to that of the McFarland scale # 0.5) were seeded on the surface of Mueller Hinton agar. Agar wells were applied and different concentrations of the samples (50; 25; 12.5; 6.25; 3.125 μg/mL) were applied. As controls of the experiment, penicillin/streptomycin (positive control) and Tween® 5% solution (negative control) were used. Plates were held for 30 min at room temperature and then incubated at 35° C/18h. After this period, the inhibition halos were read with a halometer and the values of the halos obtained through the averages of the halos, in millimeters, of three experiments. The results were evaluated using ANOVA with p≤0.05. Results and conclusion: OEAC presented inhibitory activity on the growth of Staphylococcus aureus, where it was able to inhibit the growth of this strain when in the concentration of 50 μg/ml, with inhibition halos H = 7.3 ± 0.06 mm (media ± S.E.M.) OEAC at 25 μg/mL showed inhibition halo of H = 2.5 ± 0.22 mm. In view of these results, it is concluded that pure OEAC has a higher antimicrobial activity on Staphylococcus aureus ATCC 6538P favoring the effective facial cleansing formulation that will be prepared.

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CFSP261 EVALUATION OF ANTIOXIDANT CAPACITY OF RED PROPOLIS EXTRACT OBTAINED FROM ALAGOAN APIARY FLAVIA SCIGLIANO DABBUR1; CAMILA CALADO DE VASCONCELOS1; LARISSA NOVAIS AGRA DOS ANJOS1; JÉSSICA DAIANE LINO DOS SANTOS1; JADRIANE DE ALMEIDA XAVIER2; MARÍLIA OLIVEIRA FONSECA GOULART2. Centro Universitário Cesmac, Maceio - AL - Brazil; 2Universidade Federal de Alagoas, Maceio - AL - Brazil.

Introduction and objective: The botanical origin of red propolis is the Dalbergia ecastophyllum (L.) plant, located in the Alagoas mangroves. This propolis has an important functional and therapeutic alternative as it presents bioactive properties differentiated from those obtained in other regions of the country. In the present study, the influence of the extraction method to obtain propolis extracts was evaluated by the content of phenolic compounds and antiradical activity by DPPH. Materials and methods: The extracts were conducted by simple extraction (maceration) for 48 hours (extract A) and by maceration followed by ultrasound with 24 hours total extraction (extract B). The absorption spectrum in the UV-Vis region (200 to 400 nm) was obtained by simple extract dilution. The content of phenolic compound was evaluated by the Folin-Ciocalteu spectrophotometric method at 760 nm from the gallic acid calibration curve (6 to 24 μmol L-1) and the results were expressed as milligrams of gallic acid/equivalent grams of dry extract. The propolis extracts antioxidant capacity evaluation was carried out at 516 nm in 30 minutes by monitoring the reduction of the DPPH radical to DPPH-H (diphenyl-picryl-hydrazine) as it IC 50 (the necessary extract amount to reduce free radical DPPH by 50 %). Results and conclusion: The content of phenolic compound for extracts A and B was 462.25 ± 14.99 and 520, 55 ± 42.75 mgEAG/g dry extract, respectively. The activity of the DPPH radical in the 30-minute reaction time was 32.12 % for extract A and 28.08 % for extract B and the IC50 was 10.06 and 10.08 μg mL-1, respectively. The results showed that the extracts antioxidant potential obtained by different methodologies did not show any variation, however, the ultrasound method reduced extraction time by half and has the benefits of being a green technology. Financial support or fellowship: Programa Semente de Iniciação Científica - PSIC/CESMAC.

EVALUATION OF CYTOTOXIC POTENTIAL OF XYLITOL

CFSP262

NAYANNE LARISSA CUNHA; RICARDO ANDRADE FURTADO; OSMAR QUINTINO SIQUEIRA JÚNIOR; DENISE CRISPIM TAVARES; WILSON ROBERTO CUNHA; MARCIO LUIS ANBDRADE E SILVA. Universidade de Franca, Franca - SP - Brazil. Introduction and objective: Xylitol is a low calorie sweetener and has been used as a sweetening agent in human food. It has gained widespread acceptance as an alternative sweetener because of its role in reducing development of caries. It is found naturally in many fruits and vegetables and is also produced by the human body during normal metabolism. Xylitol is approved for use in food, pharmaceuticals and oral health around the world. Xylitol is used in foods such as chewing gum, firm and pharmaceutical bullets and in oral and pharmaceutical products such as throat lozenges, cough syrups, infantile chewable multivitamins, toothpastes and mouthwashes. Objective: Evaluation of the cytotoxic potential of xylitol. Materials and methods: Evaluation of the cytotoxic activity of xylitol was performed using the In Vitro Toxicology Colorimetric Assay - Kit XTT (Roche Diagnostics). For the experiments, 1 × 104 V79 cells (Chinese hamster lung fibroblasts) were seeded in microplates with 100 μL culture medium (HAM F10/DEMEM isolated 1:1) supplemented with 10% fetal bovine serum. After 24 h, the cells were treated with 12 different concentrations of xylitol, ranging from 0.016 to 32.8 M for 24 h. Results and conclusion: Results and conclusion: Treatments with xylitol showed percentages of cell viability that did not differ significantly from those of the negative control group. Therefore, xylitol showed no cytotoxic effect, under the experimental conditions used. Financial support or fellowship: FAPESP; CAPES; CNPq, FAUNA & FLORA

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CFSP263 EVALUATION OF FORMULATION AND PROCESS VARIABLES INTERFERENCE IN SOLID LIPID NANOPARTICLES PRODUCTION BY HIGH PRESSURE HOMOGENIZATION CAROLINE CIANGA SALES; NADINE GONÇALVES MASCARENHAS; PATRICIA SANTOS LOPES; VANIA RODRIGUES LEITE E SILVA; NEWTON ANDRÉO FILHO. Universidade Federal de São Paulo, Diadema - SP - Brazil. Introduction and objective: Solid Lipid Nanoparticles (SLN) are alternative carriers to load active substances over traditional formulations. These systems present advantages such as sustained release of drugs, physical-chemical stability, and minimization of side effects. The choice and amount of the surfactant promote the maintenance of the molten droplets in the nanometric range and influence the characteristics of the SLN. Objectives: Evaluating the influence of formulation variables (surfactants) and process (time x processing cycles) on solid lipid nanoparticles obtained by high-pressure homogenization technique in terms of medium size, dispersity, and physical stability. Materials and methods: To obtain the nanostructured systems an emulsion containing glyceryl monostearate (MEG) as lipid phase, surfactant, and polyvinyl alcohol and water was prepared, in the mass proportions of 5:1:1:93. Sodium lauryl sulfate (anionic), Cetyltrimethylammonium chloride (cationic) and poloxamer 407 (nonionic) were used as surfactants. 100 g emulsion was prepared by heating and mechanical agitation of the aqueous and oily phases (10min, 70°C), which were subsequently subjected to highpressure homogenization for 5, 10 or 15 minutes in a continuous cycle or by the number of corresponding passages (3, 6 or 9 passages, respectively). All formulations were packed in vials and stored in a refrigerator at 8 °C. The measurements of average size and dispersion were performed by laser diffraction for times of zero, 30 and 60 days. All experiments were performed in triplicate. Results and conclusion: The high-pressure homogenization method allowed the SLN to be obtained with the average size ranging from 190 to 240 nm for the different surfactants. It was possible to verify that production using 3 and 6 passages of homogenization was not able to provide adequate systems for the distribution of size and dispersity, while the use of 9 passages provided adequate results and equivalent to the single cycle production regardless of the processing time. It was also verified that the cationic surfactant led to the obtaining of SLN with larger particle size and presented the lowest physical stability having a significant gain in average size and increase of the dispersity after 60 days. In contrast, the systems obtained with anionic and non-ionic surfactants were stable throughout the study period, showing no variation of these parameters. It was concluded that the SLN obtained by single cycle, regardless of the time used, was superior to the use of multiple passages and that the cationic surfactant was shown to be less effective at obtaining SLN of reduced size and adequate uniformity, besides low physical stability. Financial support or fellowship: Fundação de Amparo a Pesquisa do Estado de São Paulo CFSP264 EVALUATION OF SUN PROTECTION FACTOR CONSIDERING REAL USE OF PANCAKE FORMULATION LUCAS PORTILHO GERALDO1; ANDRÉ DA SILVA CASAVELHA2; LEONARDO DE CAMPOS SIA3; GUILHERME HENRIQUE JERÔNIMO DE SOUZA3. Unicamp, Campinas - Brazil; 2Ipupo Eduacacional, Campinas - SP - Brazil; 3Ipupo Educacional, Campinas - SP - Brazil.

Introduction and objective: Photoprotectors in Brazil must prove the Solar Protection Factor (SPF) before being placed on the market. The SPF of a product is determined through in vivo tests, according to the ISO 24444 methodology, as described in RDC 30/2012 of the ANVISA. The test considers the mass (mg) of photoprotector in relation to the area (cm2). The amount used in the test is 2 mg/cm2, however, published studies have already shown that the amount applied by consumer is less than that used in the test. The aim of this research was to evaluate whether the real quantity of a photoprotector in cosmetic form of pancake with SPF 50, when applied by the consumer, provides the SPF declared on the labeling. Materials and methods: Ten volunteers were selected to apply the photoprotector. Posteriorly, the mass applied by the volunteers was evaluated through weighing in semi-analytical balance. The SPF test methodology ISO 24444 was performed with the actual amount applied 0.1582 mg/cm2. Results and conclusion: Considering the actual quantity applied, the SPF value found was 6.8 instead of 50 as stated on the label. Therefore, the actual use of photoprotector in the pancake form did not reproduce the same photoprotection when compared to the SPF result made before the product launch. Thus, the amount used in the effectiveness tests may not match the actual amount, generating a false sense of protection. This matter needs to be discussed and publicized because the consumer may feel protected and consequently improperly exposed to the sun, at the risk of developing damage caused by solar radiation Financial support or fellowship: Ipclin

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CFSP265 EVALUATION OF THE ANTIFUNGAL ACTIVITY OF TRIAZENE AND THE PLATINUM SOLUTIONS OF PIROXICAM AND MELOXICAM FERNANDA BARRETO DA SILVA1; LUNA MAYRA DA SILVA E SILVA1; PAULO CESAR MENDES VILLIS2; PATRICIA DE MARIA FIGUEIREDO1. Universidade Federal do Maranhão, São Luís - MA - Brazil; 2Universidade Ceuma, São Luís - MA - Brazil.

Introduction and objective: The frequency of fungal infections has increased over the years and it is necessary to develop new antifungal agents. This paper contributes to expanding studies on the antifungal activity of triazene, piroxicam and meloxicam platinum. Materials and methods: The sensitivity of the triazene and platinum solutions was determined against fungal environmental isolates producing aflatoxin (Fusarium oxysporum, Cladosporium spp, Penicillium janthinellum) and clinical isolates of Candida species (C. albicans, C. glabrata, C. parapsilosis, C. tropicalis) from standard microbiological assays for the tests. For piroxicam and meloxicam solutions platinum, the lowest MIC was 1: 32mg/ml for C. albicans and C. parapsilosis; for C. tropicalis and C. glabrata the lowest MIC was 1: 64mg/ml. In triazene solution, the lowest MIC found for all genera Candida was 1: 64mg/ml. For filamentous fungi, MIC was 7% mg/ml it inhibited the growth of the fungi for 7 days. Results and conclusion: The results demonstrate the potential of tested compounds for the control and inhibition of fungi of environmental and clinical interest. Financial support or fellowship: CAPES CFSP266 EVALUATION OF THE ANTIMICROBIAL ACTIVITY OF STALK EXTRACT OF CASSIA FISTULA - GOLDEN RAIN ELISANGELA RODRIGUES DA SILVA; HALANE MOREIRA DA SILVA; LARISSE MOTA MARQUES; ANDRÉA BESSA TEIXEIRA; TAIANA MAGALHÃES PIERDONÁ. Faculdade Metropolitana da Grande Fortaleza- Fametro, Fortaleza - CE - Brazil. Introduction and objective: Cassia fistula (CF), popularly known as “golden rain”, is one of more than 600 species of the genus Cassia (Leguminosae) CF demonstrating antitumor, antioxidant and anti-inflammatory effects, among others. The objective of this work was to evaluate the in vitro antimicrobial activity of a CF stem extract on strains of Staphylococcus aureus (SA). Materials and methods: The hydroalcoholic extract of the CF stem was obtained by the percolation method in ethanol (70%) shortly after drying the vegetal drug in a stove (40ºC, 24h). The antimicrobial potential of the extract (10 - 0.312mg/mL) was tested against the SA (ATCC 6538P) strain by the agar diffusion method. For this, a microbial suspension (approximately 106 CFU/mL) was seeded in 3 petri dishes containing Mueller Hinton agar. Each sample or positive control (penicillin/streptomycin) or negative (distilled water) was dispensed into a well in the agar and incubated at 35ºC/24h. Inhibition halos were measured in millimeters and the mean halos for each sample were calculated. Results and conclusion: CF extract showed antimicrobial activity on SA, where it was able to inhibit the growth of this strain HI = 0.2±0.1 (10mg/ml), 0.13±0.32 (5mg/ml), 0.2±3.39 (2.5mg/ml), 0.83±0.20 (1.25mg/ml) and 0.76±0.05 (0.625mg/ml). It was observed that the concentrations of 1.25 and 0.625 obtained a greater inhibition halo, which justifies the fact that the lower concentration shows an ease of diffusion in the agar medium and consequently a better antimicrobial activity. Thus, it is suggested that the antimicrobial activity is also carried out by means of dilution methods in culture broth, in order to determine the minimum inhibitory concentrations. The results obtained in the present study suggest an antimicrobial potential of CF stem extract on SF strains. Financial support or fellowship: Faculdade Metropolitana da Grande Fortaleza - Fametro

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CFSP267 EVALUATION OF THE ANTIOXIDANT ACTIVITY OF TRANS-CHALCONE MICROENCAPSULATED IN ULCERATIVE COLITIS MODELS LAISA GIORDANI VICENTE; IGOR REBECH; CARLA FABIANA SOUZA GUAZELLI; RUBIA CASAGRANDE; SANDRA REGINA GEORGETTI; ISABELA SALOMÃO LUDWIG; WALDICEU APARECIDO VERRI JUNIOR; MARCELA MARIA BARACAT. UEL, Londrina - PR - Brazil. Introduction and objective: Ulcerative colitis (UC) is a chronic gastrointestinal tract disease and its therapy is based on the suppression of inflammation and mucosal healing, but with low efficacy. Previous studies have shown antioxidant and antiinflammatory activity of chalcones, which demonstrates a promising therapy for UC. Therefore, microcapsules were produced in different drug polymer proportions in order to strengthen the biological activity of trans-chalcone (T) as well as reduce the dose of the drug and promote a specific colon release. Objectives: To evaluate the antioxidant effect of trans-chalcone-loaded microcapsules (MT) and trans-chalcone not microencapsulated on acetic acid-induced colitis in mice. Materials and methods: Inert microcapsules (IM) and two microencapsulated formulations (MT 1:3 e MT 1:5) were prepared by complex coacervation process containing pectin/casein polymer loaded with T. The antioxidant capacity was measured by ferric reducing antioxidant power (FRAP), ABTS radical cation scavenging property and reduced GSH assay. Results and conclusion: MT1:3 (0.3 mg/kg) treatment with different doses of IM and T significantly prevented colon antioxidant depletion in acetic acid-induced colitis, improving the FRAP, ABTS radical cation scavenging property and GSH levels, preserving tissue from oxidative damage, which is directly related to intestinal inflammation and demonstrates the importance of the pharmaceutical development of modified release systems to reduce the dose of drugs for antioxidant effects. However, further investigations are needed in order to evaluate whether a similar efficacy can be achieved in other models of experimental colitis and in clinical application. Financial support or fellowship: CNPq; CAPES CFSP268 EVALUATION OF THE ANXIOLYTIC ACTIVITY OF ETHANOLIC EXTRACT FROM THE SHELLS OF VATAIREA MACROCARPA (BENTH) DUCKE IN MICE FERNANDA SILVA GRACIANI1; JOYCE ALENCAR SANTOS1; CLÁUDIA ANDREA LIMA CARDOSO2; CÂNDIDA APARECIDA LEITE KASSUYA1. Ufgd, Dourados - MS - Brazil; 2Uems, Dourados - MS - Brazil.

Introduction and objective: The Vatairea macrocarpa (leguminoseae) popularly known as Angelim-do-Cerrado, is widely distributed in the Brazilian savannah. The objective of this work was to evaluate the anxiolytic activity of ethanolic extract of the shells of V. macrocarpa in mice. Materials and methods: After 1 hour of fasting, the animals were divided into negative control animals (saline), V. macrocarpa 100 and 300 mg/kg (v.o) and positive control 10 mg/kg diazepam (s.c.). The animals were evaluated for 5 minutes in Elevated plus-maze test (EPM) and Open Field (OF). Results and conclusion: The treated animals entered less (24.5%) in the closed arms compared to the control animals. Regarding the open arm, there was an increase of 6.4; 8.3 and 8.8 times longer in the residence time of the groups diazepam, V. macrocarpa 100 and 300 mg/kg respectively in comparison to the control, in the EPM test. The animals presented no difficulty in locomotion (OF), the treated animals traveled less distance in relation to the control, in addition, they transited 2.5 times more in the internal region in comparison to the control. In several parameters, the group treated with the extract, obtained results similar to the standard drug. The ethanolic extract of V. macrocarpa shells presented an anxiolytic effect in the experimental models proposed. Financial support or fellowship: FUNDECT; CNPq.

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CFSP269 EVALUATION OF THE PHENOLIC COMPOUND AND TOTAL FLAVONOID CONTENT PRESENT IN THE ETHYL ACETATE FRACTION OF LEAVES OF MIMOSA CAESALPINIIFOLIA IN DIFFERENT COLLECTIONS MARCO ANTONIO DE MOURA JUNIOR; MARCELO JOSÉ DIAS SILVA; WAGNER VILEGAS. Instituto de Biociências – Câmpus do Litoral Paulista/Unesp, São Vicente - SP - Brazil. Introduction and objective: Mimosa caesalpiniifolia (Fabaceae), popularly known as “sabiá” or “hedgehog”, is an evergreen tree of multiple use in Brazil, native to the Northeast of Brazil and can reach 10m in height, indicated against inflammatory diseases, antimicrobial and infections. The main classes of secondary metabolites are the phenolic compounds, which have a chemical structure formed with a benzene ring linked to a hydroxyl radical, in this class we can highlight the flavonoids that present high pharmacological potential. Objectives: To evaluate the content of phenolic compounds and flavonoids present in the ethyl acetate fraction (Fr-AcOEt) of Mimosa caesalpiniifolia leaves in different collections. Materials and methods: The leaves were collected in the municipality of Alfenas-MG, in the period of May 2016 and compared quantitatively to an earlier study by Achkar et al. (2015) with collection in 2012. After collection, the material was subjected to extraction by exhaustive percolation. Fr-AcOEt was obtained by liquid-liquid partition of the 70% (v/v) hydroethanolic extract. For the determination of phenols and flavonoids, the methods of Folin-Ciocalteu and aluminum chloride proposed by da Rocha et al. (2015) were used. Gallic acid and quercetin (Sigma) were used as standards. Results and conclusion: The phenolic content of Fr-AcOEt showed significant amounts (61.51 ± 1.9 mg of EAG .g-1) when compared to gallic acid. In both collections, the values were very close, evidencing the uniformity of production of these metabolites even in different years. In flavonoids, a large discrepancy of values (4.28 ± 0.14 mg of EQ .g-1) was observed in relation to the collection in 2012 (58.16 ± 0.5 mg of EQ .g-1) taking as hypothesis the collection period may not be the most adequate for concentration of flavonoids or climatic factors (seasonality) time, day and distribution of collected points. Financial support or fellowship: BIOTA-FAPESP (09/52237-9), FAPESP (15/21479-8; 16/14418-5), UNESP-IB/CLP CFSP270 EVALUATION OF THE IN VITRO BACTERICIDAL ACTIVITY OF THE DYNAMIZED ESSENTIAL OIL OF Aloysia polystachya BEFORE AND AFTER FREEZING TALITA THOMAZ NADER1; CAMILA YAMASITA HENRIQUE1; ALEXANDRE HENRIQUE LEONEL1; ANA MARIA SOARES PEREIRA2. Homeopatia Brasil, Franca - SP - Brazil; 2Unaerp, Ribeirão-Preto - SP - Brazil.

Introduction and objective: Essential oils obtained from plants are complex mixtures of substances and it is suggested to keep them under refrigeration for better conservation. On the other hand, homeopathic mother tincture prepared from the plant is kept at room temperature. Thus, this work aimed to evaluate if the freezing process changes the in vitro bactericidal activity potential of the homeopathic preparation Aloysia polystachya 1 CH against Escherichia coli. Materials and methods: The inoculum of E. coli ATCC 25922 was standardized on a spectrometer and distributed in a 96-well plate with Aloysia polystachya 1 CH, prepared according the Brazilian Homeopathic Pharmacopoeia (FHB, 3rd edition) from A. polystachya essential oil. An aliquot of this homoeopathic preparation was frozen and after 40 days it was submitted to the same methodology for evaluation of the antibacterial activity. After incubation, the plates were read with triphenyltetrazolic (TTC) (Vetec®).” Results and conclusion: The results of the in vitro evaluation showed that the freezing process did not change the bactericidal activity of the dynamized essential oil of Aloysia polystachya 1 CH against Escherichia coli. Therefore, freezing can be considered as one viable method of preserving dynamized essential oils.

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CFSP271 EVALUATION OF THE LAST 5 YEARS OF MICROBIOLOGICAL ANALYSES OF A LABORATORY FOR THE CONTROL OF MEDICINES, COSMETICS, DOMESANITARY PRODUCTS, RELATED PRODUCTS AND THEIR RESPECTIVE RAW MATERIALS - CONFAR/USP. GABRIELA CORREA CARVALHO1; MARINA DE SOUZA BRAGA1; CLEIDE MARIA DE OLIVEIRA AMARAL2; LUANA CRISTINA VAZ FORMAGGIO2; ROGERIO TAKAO OKAMOTO2; ELISABETE ALMEIDA BARBOSA ANTUNES2; DANIELA DAL MOLIM GHISLENI2; TEREZINHA DE JESUS ANDREOLI PINTO1. FCF-USP, SP - SP - Brazil; 2Confar-USP, SP - SP - Brazil.

Introduction and objective: For more than 44 years, CONFAR began activities with the objective of integrating teaching, research and university extension. The university extension is one of the actions for academic formation in order for academics to experience activities, which are in parallel with the reality lived in the community, providing activities that add knowledge and experience of learning “doing” for professional qualification. In this perspective, the team is formed of professors, undergraduate/postgraduate students, and laboratory technicians in developing tests, research and studies in the field of pharmacy. Faced with this, the objective of this study was to quantitatively evaluate the demand of products sent by the community to CONFAR in the last 5 years. Materials and methods: Microbiological analysis control sheets that were requested and processed by CONFAR in the period from 2012 to 2016 were used for the analysis. Quantitative data were used and the samples were categorized into 9 types, and then the analyses were performed within each category. Results and conclusion: During the 5 years analyzed, an average of 433 analyses/year (± 250.9), it was observed that medicines were the most prevalent among this period (58.6% ± 19.4) and requested type of analysis: sterility, dosing, counting and endotoxin; The second most requested category was medical devices (25.25% ± 13.96) with sterility and counting analyses. Another important figure was in relation to the approved and disapproved analyses, being 98.93% and 1.06% respectively. Considering all this information, it is possible to conclude the great importance the CONFAR laboratory has for the community and because it is part of a University, it contributes positively to the triad: teaching, research and extension. Financial support or fellowship: FipFarma. CFSP272 EVALUATION OF THE PHYSICAL-CHEMICAL QUALITY OF MEDICINES CONTAINING DICLOFENAC POTASSIUM FERNANDO CESAR QUEIROZ DA SILVA; JESSICA VERTUAN RUFINO; MARLENE MARIA FREGONEZY NERY. Universidade Estadual de Londrina - Uel, Londrina - PR - Brazil. Introduction and objective: Like any drug, Potassium Diclofenac needs a standard quality before becoming available in the drugstore, so that people can use it, but is necessary to go through physical and physicochemical tests, which are recommended by the official compendia, to prove such efficacy and pharmaceutical quality. Potassium Diclofenac is a benzeneacetic acid derivative, designated chemically as 2-[(2,6- dichlorophenyl)amino] benzeneacetic acid monopotassium salt. Diclofenac is a potent nonsteroidal anti-inflammatory drug and is widely used in cases of chronic and acute inflammation. In Brazil, the pharmaceutical market offers several presentations of this drug to the consumer, in various strengths, one of which is 50 mg, so the consumers can make choice considering the drug cost and the quality. This study was performed to analyze the drug Potassium Diclofenac 50 mg tablets in five different pharmaceutical samples in order to verify the quality of these products. Materials and methods: The study was made by conducting a study of quality control parameters like weight variation, friability (erveka friabilator), hardness (erveka hardness tester), dissolution tests (variam). Diclofenac sodium (50 mg) of five different formulations. The products were coded as R1(reference product), S1 (similar drug) and G1, G2, G3 (generic drugs). The reagents used were Potassium dihydrogen phosphate, NaOH 0,1 mol L-1 and freshly distilled water. Results and conclusion: The drugs of the five evaluated companies were approved in the tests of average weight, hardness, friability, content and uniformity of dose. In the dissolution test, the reference and similar medicine presented the highest quantity of drug dissolved (92.1±8.4% and 95.25±1.33%), followed by generic G2 (84.4±9.47%). However, in the dissolution test samples G1 (82.61±27.16%) and G3 (49.61±7.75%) did not meet the minimum requirements. It was possible to see through the physicochemical tests that there is a need for greater quality control in manufacturing of these drugs and tests for bioequivalence and bioavailability should be undertaken to prove its efficacy and safety.

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CFSP273 EVALUATION OF THE STABILITY OF ORAL SOLUTIONS OF FUROSEMIDE IN PHARMACEUTICAL DERIVATIONS ADMINISTERED AT A MATERNITY HOSPITAL IN FORTALEZA-CEARÁ LUANA ARAÚJO SILVA1; SUZANA BARBOSA BEZERRA2. Undergraduate Student in Pharmacy at Faculdade Metropolitana da Grande Fortaleza – Fametro, Fortaleza - CE - Brazil; Professor of Faculty of Pharmacy at Faculdade Metropolitana da Grande Fortaleza - Fametro, Fortaleza - CE - Brazil.

1 2

Introduction and objective: Furosemide is an important drug used in the clinic as a diuretic and it is prescribed for adaptations of its original pharmaceutical form in the hospital environment, since the industrialized form often does not meet the prescribed dose. From the pharmaceutical derivation, it is not necessary to know the expiration date of these solutions, which may compromise their stability, it being necessary to carry out physical-chemical and microbiological quality control tests. This study aims to evaluate the stability of formulations of furosemide in oral solution, prepared and administered in a Maternity Teaching Hospital located in the city of Fortaleza, Ceará. Materials and methods: The study was carried out at the Laboratório de Tecnologia Farmacêutica, Fametro, using samples of furosemide solutions of 1mg/ml, provided by the compounding laboratory of the Walter Cantídio Hospital Complex (HUWC/ MEAC). The analyses were performed on day zero (day 1 of preparation), day 15 and 30, in which physical-chemical tests were performed, such as appearance, density, pH and drug dosing; microbiological assays (by agar-Pour Plate method) and the accelerated stability test - in a refrigerator: 2ºC, in oven (40ºC) and on a shelf (out of direct sunlight 25ºC). Results and conclusion: Throughout the study, the appearance of the furosemide samples showed no visible changes and the values of density remained constant (1 g/ml in all samples). The pH of the furosemide solution at time zero was 10 and after 15 and 30 days of storage the value found was 9 (for both refrigerator, stove and shelf). The determination of the furosemide content from the spectrophotometry obtained in the samples after 30 days conditioned in refrigerator, shelf and oven showed, respectively, 85.58%, 80.85% and 83.21% of drug. For the microbiological counting of the solutions, 5.5 × 10¹ CFU/ml were found in the microbial plate count test for all samples, regardless of location and storage time. From the results obtained, it was possible to verify that the magical formulations of furosemide showed slight changes after 15 days of storage, mainly with exposure to light, which guides a period of validity up to this period without compromising patient safety.

FORCED DEGRADATION STUDY OF BESIFLOXACIN IN OPHTHALMIC SUSPENSION

CFSP274

ELIANE GANDOLPHO TÓTOLI; HÉRIDA REGINA NUNES SALGADO. Universidade Estadual Paulista Júlio de Mesquita Filho, Araraquara - SP - Brazil. Introduction and objective: Bacterial conjunctivitis has a high impact on the health of the population, since it represents more than a third of ocular pathologies reported by health services worldwide. There is a high incidence of bacterial resistance to the antimicrobials most commonly used for the treatment of this pathology. In this context, besifloxacin stands out. This is a fourthgeneration fluoroquinolone, which was developed exclusively for topical ophthalmic use, presenting lower risk of developing resistance due to its reduced systemic exposure. Considering the importance of this drug for the global scene, the aim of this work was to perform a forced degradation study of besifloxacin in ophthalmic suspension. Materials and methods: Sample solutions of besifloxacin were subjected to forced degradation by acidic, basic, thermal, oxidative and photolytic conditions and were evaluated by HPLC. Thermal degradation was performed at 60° C and the others at room temperature, for 7 days. HPLC method employed as mobile phase water: ethanol (68:32, v/v) with 0.1% formic acid, at 0.8 mL min-1. A C18 column was used as stationary phase and the UV detection was performed at 290 nm. Kinetic degradation of besifloxacin under all stress conditions was evaluated through the graphical method. Results and conclusion: Besifloxacin showed itself to be more stable in thermal and acidic conditions, and the maximum degradation was observed in photolytic condition. Photolytic, oxidative and alkaline degradations exhibited first-order reactions with rate constants (k) of 0.0615, 0.0088 and 0.0048 h-1, respectively. It means that it degrades approximately 6.15%, 0.88% and 0.48% per hour, respectively, and the rate of degradation is proportional to the concentration of only one reagent. The drug concentration decreases exponentially as a function of time. On the other hand, due to the low degradation of besifloxacin under thermal and acidic conditions, it was not possible to estimate the kinetics of the reaction. Financial support or fellowship: CNPq (437489/2016-7); PADC-FCF; Bausch & Lomb.

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CFSP275

REGIANI REBOLI CARDOSO; ANDRIELI TAISE HAUSCHILDT; MADILEINE FRANCELY AMÉRICO. UFMT, Barra do Garças - MT - Brazil. Introduction and objective: The use of synthetic steroids concomitant or not with physical exercise has been increasing aesthetic improvements and athletic performance, although its side effects on the gastrointestinal tract are controversial (Silva; Czepielewski, 2002) (Busardòet al., 2015). The aim of this study was to evaluate the effects of nandrolone decanoate treatment on the gastrointestinal transit in rats. Materials and methods: Fourteen male Wistar rats were divided into: control group (C, n = 7) and anabolic group (DN, n = 7). The anabolic-treated group received nandrolone decanoate (5 mg/kg, i.m.) 5 times a week for 4 weeks. The control received vehicle (peanut oil) by the same route and period. Gastrointestinal transit was evaluated by means of Alternating Current Biosusceptometry (ACB) - a non-invasive biomagnetic technique and free from ionizing radiation - on treatment days 01, 14 and 28. Rats were fasted for 12 hours prior and then fed with magnetically labeled ration. The stomach and cecum regions being demarcated and monitored by the ACB sensor at intervals of 15 minute for 6 hours. Thus, mean gastric emptying time (MGET), mean cecal arrival time (MCAT) and mean small intestinal transit time (MSITT) were quantified. Results and conclusion: It was observed that MGET of the anabolic group was significantly faster in comparison to the control (109.1 ± 7.4min), both in the 14th (85.1 ± 7.5 min) and 28th day of treatment (85.4 ± 8.2 min), p <0.001. In the temporal comparison, this change in gastric emptying is already perceived at 14 days and remains until the end of treatment, compared to the same group at day 01 (p <0.01). Regarding MCAT, there was no significant difference between groups. In the MSITT, animals treated with nandrolone has slowed intestinal transit on the 14th day of treatment (165.9 ± 12.7 min) in the temporal comparison with day 01 (136.6 ± 13.7 min) (p <0.05 ). In conclusion, treatment with nandrolone decanoate disturbs gastrointestinal function in terms of propulsion, which may modify digestive and absorptive nutrients and drugs processes and result in the appearance of side effects. Financial support or fellowship: CNPq CFSP276 GENETIC TOXICOLOGICAL ASSESSMENT OF ETHANOLIC EXTRACT FROM THE LEAVES OF ALTERNANTHERA MARITIME IN MICE FERNANDA SILVA GRACIANI; JOYCE ALENCAR SANTOS; CÂNDIDA APARECIDA LEITE KASSUYA. UFGD, Dourados - MS - Brazil. Introduction and objective: Today, many plants are being used as medicines without a prior study on the real health effect, whether beneficial or harmful. The objective of this work was to evaluate toxicogenetic safety of the ethanolic extract from the leaves of Alternanthera maritime. Materials and methods: Samples of peripheral blood (20 µL) were collected for the micronucleus test (mutagenicity) at 24, 48 and 72 h after administration of: saline (negative control), cyclophosphamide (positive control) and A. maritime (1750, 175 and 17.5 mg/kg). In the 24h blood was also collected for the comet assay (genotoxicity). Organs were collected for splenic phagocytosis and apoptosis assays. Results and conclusion: In the genotoxic activity, the positive control had an average increase of frequency 10.3 times of DNA damage. In the test of mutagenicity, the control positive had 4, 3.4 and 2.3 times greater in the presence of micronuclei respectively in the times tested. Cyclophosphamide showed higher proportion of apoptotic cells in all organs studied and caused around 35-fold increase in splenic phagocytosis. In all data, the positive control was compared to the negative control and to the extract, and this did not present statistical difference to the negative control (p < 0.001). The ethanolic extract from the leaves of Alternanthera maritime was not genotoxic or mutagenic. Financial support or fellowship: FUNDECT; CNPq.

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CFSP277 GRADIENT ELUTION OF HYDROALCOHOLIC EXTRACTS OF DIFFERENT PARTS OF THE SPECIES PHYSALIS ANGULATA LINN. INGRID SILVA DE OLIVEIRA1; AFONSO RAFAEL NASCIMENTO E SILVA1; SANT CLAIR SILVA DOS SANTOS1; CHRISTIAN NERI LAMEIRA1; MILTON NASCIMENTO DA SILVA2; DANILA TERESA VALERIANO ALVES1. Faculdade Integrada Brasil Amazônia - Fibra, Belém - PA - Brazil; 2Universidade Federal do Pará - Ufpa, Belém - PA - Brazil.

Introduction and objective: Physalis angulata Linn. (Camapú), is a species rich in vitaesteroids (physalins) and infusions of the plant are widely used for the treatment of several diseases, among them, cancer. In the literature, the antitumor activity of D, G and B physalins has been demonstrated. Therefore, the objective of this work is to perform chemical profiles of hydroalcoholic extracts from different parts of the plant (stalk, fruit, root and leaf) in order to verify the relative quantities of these three substances present. Materials and methods: The physalins D, G and B were isolated from the fractionation of the ethanolic extract obtained by Sohxlet. Then hydroalcoholic extracts of the different parts of the plant were prepared, separately. The methodology consisted of an aliquot of 100 mg of botanical material in 10 mL of the H2O/EtOH 50% system in triplicate. The solutions were subjected to the ultrasonic bath heated to 69°C in 4 batches of 10 minutes, being filtered in nylon membranes. Each sample was treated by SPE, followed by solvent evaporation, reconstituted in 1 mL of the H2O/ACN 50% system and then injected into the HPLC. The chromatographic method was set at 1 mL/min, 60 minutes analysis, analytical Gemini C18 column (250 mm x 4.6 mm, 5 μm) and the detector operated at wavelength of 227 nm. Results and conclusion: The three structures had their structures determined from 1H and 13C spectroscopic analyses and confirmed by literature data. Regarding the profiles of the different parts of the plant, it was observed that the samples of stalk, leaf and root presented the physalins D and B as majority, whereas the samples of fruit only the physalin D as majority. In relation to the concentration of the substances, the leaf samples were those with the highest concentrations and the fruit samples with the lowest concentrations. The wavelength was monitored at 227 nm. Financial support or fellowship: CAPES – UFPA CFSP278 HPLC-UV METHOD AS A FASTER ALTERNATIVE FOR THE DETERMINATION OF AZITHROMYCIN CONTENT IN A POWDER FOR ORAL SUSPENSION FERNANDA FERNANDES FARIAS; ELLEN GAMEIRO HILINSKI; VALERIA ADRIANA PEREIRA MARTINS; ADRIANA APARECIDA BUZZO ALMODOVAR; LUZ MARINA TRUJILLO. Instituto Adolfo Lutz, São Paulo - SP - Brazil. Introduction and objective: The antimicrobial potency must be in compliance with the parameters specified by official compendia in order to promote an effective action on microorganisms. The Brazilian Pharmacopoeia establishes agar diffusion as an official method for azithromycin powder for oral suspension assay determination. However, this method is a bacterial growth dependent, which hinders the release of results in a short time. The objective of this work was to develop a faster method using HPLC and also perform the analysis using the HPLC-UV and agar diffusion methods to compare the results of azithromycin assay between both methods. Materials and methods: A sample batch of azithromycin powder for oral suspension 200mg/5mL was used for the tests. The physicochemical method has been carried through an isocratic HPLC-UV method using a reversed phase 250 x 4.6 mm C18 (5μm) column at 50°C, a mobile phase constituted of methanol and 0.03M potassium phosphate buffer pH 7.5 (80:20) at a flow rate of 1.5 mL/min and detection at 210nm. The microbiological method was performed according to Brazilian Pharmacopoeia 5th edition. Results and conclusion: The azithromycin content result by HPLC was 190.74 mg/5mL (95.4% of declared content) and by agar diffusion was 184.5 mg/5mL (92.3% of declared content). The difference between the results was 3.4%. The HPLC-UV method was responsive, economic and gave a sharp peak of azithromycin at a retention time of 9.4 minutes. Moreover, the HPLC method was more sensitive, accurate and faster than the microbiological method, which requires an incubation period of 16-18 hours to obtain the results. Therefore, the developed method can be a faster alternative for the azithromycin determination in pharmaceutical formulations.

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CFSP279 IDENTIFICATION AND DETERMINATION OF SPECIFIC CHEMICAL COMPOUNDS OF COMMERCIAL SAMPLES OF SENNA ALEXANDRINA MILL JULIANA CRISTINA MANSANO; VITHORUGO S. BETTINI; ADEMIR SALVI JÚNIOR. Centro Regional Universitário de Espírito Santo do Pinhal - UNIPinhal, Esp. Sto. do Pinhal - SP - Brazil. Introduction and objective: Senna alexandrina Mill, belonging to the family Leguminosae, popularly known as Sene, native to Africa. The plant drug consists of dried leaves and is used in the therapy due to its laxative effect, being indicated for treatment of occasional intestinal constipation (ANVISA, 2016, p.91). It is known that the identification and quantification of markers is an important strategy for the quality control and that the quality of the plant drug can interfere in its therapeutic activity. The objective of this work was to verify the authenticity, by the identification of characteristic chemical constituents and to carry out the quantitative test of chemical constituents, by the determination of hydroxyiantracenic derivatives of plant drugs of the S. alexandrina species. Materials and methods: Desiccated leaflets of S. alexandrina were obtained commercially from Vargem Grande do Sul, SP (samples A and B) and São João da Boa Vista, SP (samples C and D), thus identified for analytical purposes. The samples were processed in a mill and submitted to analysis, in triplicate, of identification and determination of hydroxantracenic derivatives, according to the Brazilian Pharmacopoeia (2010, vol. 2, p.707-8). Results and conclusion: The results of the phytochemical tests demonstrated, through colorimetric reaction, the positivation in the identification of Sene, by the development of characteristic staining (reddish) for the analyzed samples. The content of the calculated hydroxyanthracene derivatives, expressed as senoside B, by means of visible absorption spectrophotometry, presented the mean values of 1.66%, 0.77%, 0.36% and 0.52%, for samples A, B, C and D, respectively. As evidenced, the authenticity of the samples can be verified, however, by the quantitative assay, values lower than 2.5% of hydroxyanthracene derivatives were found, this being the minimum value recommended by the Pharmacopoeia (2010, vol. 2, p.707-8). And, as these compounds are considered responsible for the therapeutic activity of the plant drug, such plant raw materials should not be marketed, or used, for medicinal purposes. Financial support or fellowship: Centro Regional Universitário de Espírito Santo do Pinhal - UniPinhal CFSP280 IMMUNOMODULATORY EFFECTS OF HOST-DEFENSE PEPTIDES ISOLATED FROM THE SKIN SECRETION OF “HYPSIBAS RANICEPS” (ANURA) CLAUDIA SOFIA DE FREITAS CORREIA POPOV; FELIPE MOREIRA BERGAMO BARROS; OCTÁVIO LUIZ FRANCO; BEATRIZ SIMAS MAGALHÃES; TAIA MARIA BERTO REZENDE. Universidade Católica de Brasília, Brasília - DF - Brazil. Introduction and objective: Amphibian skin secretion is a somewhat unique source of bioactive material. The presence of these peptides on frog skin plays a primary role in a primitive but elaborate innate host defense mechanism. These biomolecules exert diverse biological functions, ranging from antimicrobial, vasodilation, analgesic, inflammatory and neuronal activities. Recent studies show a multifunctional profile activity of such compounds. In this view, they have potential for not only antimicrobial but also immunomodulatory therapies with high pharmacological and biotechnological interest. Objectives: Analyze the effect of three cysteinecontaining peptides (AC12, Hr6b and DK16) isolated from H. raniceps on inflammatory mediators produced by RAW 264.7 cells. Materials and methods: The peptides were quantified and oxidated to the formation of disulfide bridges. Subsequently, their integrity and complete oxidation were verified by MALDI ToF/ToF. Cell viability was tested on RAW 264.7 cells in the presence of the peptides using the MTT colorimetric assay. Nitric oxide production was also quantified in the cells stimulated with LPS. IL-10, IL-12 and TNF-α cytokines of the cell culture supernatant were quantified using ELISA kits. Results and conclusion: The samples did not show a cytotoxic effect in RAW 264.7 cells even in high concentrations and decreased production of NO in RAW 264.7 cells when stimulated with LPS. Levels of IL-12 and TNF-α also decreased. Tested peptides showed potential to act as anti-inflammatory drugs, since they decreased the production of NO and anti-inflammatory cytokines without cytotoxic effect. Financial support or fellowship: CNPq; CAPES; FAPDF

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CFSP281 IMPORTANCE OF STANDARDIZATION OF DENTAL BLOCKS FOR STUDIES ON THE EFFICACY OF WHITENING FORMULATION. MARIA CIBELLE PAULI 1; MARCOS HENRIQUE PASSONI 2; GISLAINE RICCI LEONARDI 3; WALDEMIR FRANCISCO VIEIRA JUNIOR4; THAYLA HELLEN NUNES GOUVEIA4. Universidade Federal de São Paulo, Piracicaba - SP - Brazil; 2Universidade Estadual de Campinas- Fop, Piracicaba - SP Brazil; 3Universidade Estadual de Campinas- Fcf, Campinas - SP - Brazil; 4Unicamp, Piracicaba - SP - Brazil. 1

Introduction and objective: Aesthetic treatments have been increasingly frequent in dental offices. Chromatic alteration is considered a phenomenon of great complexity, since it can be influenced by several factors such as ambient lighting conditions, translucency, opacity, as well as the presence of anomalies of format, thus affecting the aesthetics of the teeth, impairing the harmony of the smile, and needing interventions. Thus, bleaching agents are being increasingly used, and with it several bleaching actives, and several formulations are being studied, in order to demonstrate their effectiveness and safety. Thus, the objective of this work was to standardize bovine teeth so that they are suitable for in vitro studies of the efficacy of dental bleaching formulations. Materials and methods: The teeth were stored in 0.1% thymol solution, after extraction. Later, the teeth were examined visually, to rule out the possibility of stains and cracks, with teeth with these characteristics being excluded. Debris were removed using a scalpel, and the teeth were cleaned with Robinson brushes and water. The teeth were then sectioned into the cervical portion, separating crown and root, using a partial double-sided diamond disc, under irrigation on rotational- (Kavo®). Then, other cuts were made in the mesio-distal and incisor-cervical sections using a metallographic cutter (Isomet 1000, Buehler), with a high accuracy diamond disk (4 “x 012x ½, Buehler, Illinois, USA). The planning of dentin (1.5 mm thick) and enamel (1.0 mm) planning/polishing was performed with silicon carbide, granulation # 600, # 1200 and # 2000 under constant irrigation, using a rotary Polymer (Arotec, Cotia, SP, Brazil). Finally, they were polished with felt, associated with decreasing granulation metallographic pastes (1 μm, ½ μm and ¼ μm). Results and conclusion: The procedures required to perform tooth standardization in order to make them suitable for the efficacy studies of bleaching formulations are well defined in the scientific literature. This is a long but very important process. Efficacy studies of bleaching formulations without the standardization of dental samples may present false results, since the use of adequate methodologies is fundamental to guaranteeing the reliability of the bleaching effectiveness on the surface of the dental enamel. Thus, interdisciplinary studies are important for the development and efficacy of evaluation of formulations that aim to act in dental bleaching. Financial support or fellowship: CAPES

IN VITRO ANTIOXIDANT ACTIVITY OF COFFEA ARABICA L. LEAVES

CFSP282

LUCIANA SEGHETO; MARIA LAURA GAZOLLA PARMA; ANA FLAVIA LAWALL WERNECK; BRUNA CELEIDA SILVA SANTOS; FERNANDA MARIA PINTO VILELA; ORLANDO VIEIRA DE SOUSA; MIRIAN PEREIRA RODARTE. Universidade Federal de Juiz de Fora, Juiz de Fora - MG - Brazil. Introduction and objective: Brazil is the world’s largest producer and exporter of coffee and the scientific studies have been mainly related to the beans due to their high consumption. However, phenolic acids such as caffeic, chlorogenic, p-coumaric, ferulic, and sinapic acids, as well as rutin, quercetin, kaempferol, and isoquercitrine have been identified in the leaf of Arabic and Bengal coffee. These compounds may have numerous pharmacological properties such as anti-inflammatory, antidiabetic, antihyperlipidaemic, and neuroprotective activities, as well as providing antioxidant and antimicrobial effect in biotic stress. Considering the great therapeutic potential, the antioxidant activity of the methanolic extract from C. arabica leaves was investigated using in vitro methods. Materials and methods: Dried and powdered leaves were extracted in methanol by static maceration for obtaining methanolic extract (ME). The antioxidant activity was evaluated by DPPH radical scavenging activity, ferric reducing antioxidant power (FRAP) and co-oxidation of β-carotene/linoleic acid assays. Results and conclusion: The Effective Concentrations 50% (EC 50) obtained in the DPPH and FRAP assays corresponded to 19.80 ± 0.06 μg/mL and 17.77 ± 0.03 μg/mL, respectively. The percentage of inhibition of lipid peroxidation determined by the β-carotene/linoleic acid assay was 52.49 ± 0.52 μg/mL. These results suggest that the methanolic extract from C. arabica leaves is an important and promising source of bioactive substances with antioxidant activity.

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CFSP283 INFLUENCE OF LIPID AND SURFACTANT COMPONENTS ON THE FEATURES AND STABILITY OF SOLID LIPID NANOPARTICLES TALITA CESARIM MENDONÇA1; SIMONE RAMOS DE CASTRO2; LIGIA NUNES DE MORAIS RIBEIRO2; GUSTAVO HENRIQUE RODRIGUES DA SILVA2; ENEIDA DE PAULA2. Universidade São Francisco, Campinas - SP - Brazil; 2Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas-Unicamp, Campinas - SP - Brazil. 1

Introduction and objective: Solid lipid nanoparticles (SLN) are colloidal systems composed of a biocompatible and biodegradable solid lipid and stabilized by nonionic surfactant, with particle sizes in the range of 50-1000 nm. The particle size, polydispersity index (PDI) and zeta potential (ZP) are crucial structural features in the physicochemical stability of SLN. These characteristics are dependent on the preparation method as well as the composition of the particles. The goal of this work was to investigate the influence of different types of solid lipids and surfactants currently used in SLN preparation on the final particles size, PDI, ZP, and on the physical stability of the SLN in short-term storage (120 days). Materials and methods: SLN were prepared by the emulsification-sonication technique. Cetyl palmitate (CP), Compritol® (COMP) and Precirol® (PREC) were used as the solid lipid materials, and Pluronic® 68, Pluronic® 127, Tween 80®, Tween 40® and Tween 20®, as surfactants. The obtained formulations were characterized in terms of size, PDI, and ZP by Dynamic Light Scattering, immediately and during 120 days of storage at room temperature. Results and conclusion: The best lipid matrix was found to be CP, which SLNs showed 319 ± 4.5 nm size, PDI < 0.23 ± 0.02, and ZP = -30.0 ± 0.52 mV, for up to 120 days of storage, followed by PREC and COMP. As for the surfactants, SLN prepared with Pluronic® 127 and Pluronic® 188 showed the smallest sizes and PDI. These results suggested that careful selection of lipids and surfactants excipients is essential for the success of SLN preparation and physicochemical stability prior to incorporation of drugs. Financial support or fellowship: CAPES; FAPESP (# 14457-5). CFSP284 INTERFERENCE OF HYDROALCOHOL SOLUTIONS USED IN THE PREPARATION OF HOMEOPATHIC MEDICINES IN IN VITRO MICROBIAL MULTIPLICATION TALITA THOMAZ NADER1; CAMILA YAMASITA HENRIQUE2; ALEXANDRE HENRIQUE LEONEL2; ANA MARIA SOARES PEREIRA3. Homeopatia Brasil, Franca - Brazil; 2Homeopatia Brasil, Franca - SP - Brazil; 3Unaerp, Ribeirão-Preto - SP - Brazil.

Introduction and objective: According to the method of preparation of homeopathic medicines, hydroalcoholic solutions are used as an inert component (FHB, 3rd edition). Thus, this study was carried out with the aim of determining which alcohol percentage in this solution is capable of interfering in the results when using the homeopathic medicine in in vitro tests. Materials and methods: Inoculates of Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922 and Candida albicans ATCC 10231 were standardized at spectrometer and added in 96-well plates (100 μL) with 30%, 20%, 10% and 5% hydroalcohol solutions (100 μL). After incubation, triphenyltetrazolic (TTC) (Vetec®) was used as a colorimetric method to determine the presence of cellular activity. Results and conclusion: Under the conditions of this study, the best concentration of hydroalcoholic solution used in bacterial tests was 5%, whereas for Candida albicans, the best concentration of hydroalcoholic solution were 20% or less. Therefore, the alcoholic percentage in the solution used in the preparation of homeopathic medicine is important and may interfere in the bacterial multiplication in in vitro experiments.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

LIP HYDRATION: CHEILITIS PREVENTION.

155

CFSP285

MANUELA LIMA LOPES SANTOS. Unisantos, Santos - SP - Brazil. Introduction and objective: Cheilitis is an inflammatory process on the lips that causes desquamation, erosion or swelling. Among the causes for cheilitis, we can mention climatic conditions, food (type of food or nutritional deficiency) and allergies. The objective of the project is to study the effects of Aloe Vera, which has high nutritive, regenerating and moisturizing action, in the use of a lip protector, which will provide an active principle of natural origin to the product developed. This attempts to diminish the labial cracks, erosions and bumps, symptoms of the mentioned disease. Materials and methods: The study was developed in the laboratories of the Institute of Scientific Research - IPECI/UNISANTOS, during the second half of 2016 and the first of 2017. The Aloe Vera plant was harvested at the UNISANTOS Farmácia Verde. All preparations and stability tests were performed in IPECI laboratories. The plant was first harvested and the glycol extract extracted by maceration, after, the extract was incorporated into several preparations of lip protectors, followed by stability and permeability tests. Results and conclusion: The preparation that most closely met the objective of the research was of a lip gloss, as it was able to penetrate the skin, which enabled the added Aloe Vera to have its medicinal moisturizing action on the lips. In the stability tests, the formulation remained stable at room temperature and cold, but under greenhouse heat at 60° C for one week there was a change in odor, weight and consistency. Financial support or fellowship: PROIN/UNISANTOS.

LIPID NANOPARTICLES CONTAINING VEGETAL BUTTERS AS CARRIERS FOR SKIN HYDRATION

CFSP286

BRUNO VINCENZO FIOD RICCIO; JOSIANE DE FÁTIMA PADILHA DE PAULA; PATRÍCIA MAZUREKI CAMPOS. Universidade Estadual de Ponta Grossa, Ponta Grossa - PR - Brazil. Introduction and objective: Lipid nanoparticles (LN) have improved features compared with conventional cosmetic formulations; their compositions based on vegetal butters have the potential of skin occlusion with rich chemical composition, which can reduce transepidermal water loss. Objectives: to develop and to characterize the LN. Materials and methods: Factorial design outline 3^3-1 formulations were developed varying the concentrations of: cocoa and shea butters and capric/caprylic triglycerides to produce the LN by high shear homogenization/ultrasound method. Physicochemical measurements were performed by dynamic light scattering (DLS), nanotracking analysis (NTA) and scanning electronic microscopyfield emission gun (SEM-FEG). Preliminary stability study (PSS) evaluated size, polydispersity index (PdI), zeta potential and pH for 15 days through thermic stress. Results and conclusion: DLS analysis showed an average size around 200 nm, PdI near 0.30 and zeta potential of -25.6 mV. NTA disclosed concentrations around 2.5x1017 nanoparticles/mL. SEM-FEG evidenced rounded shape and smooth surface. The pH values were near to 5.6 and 5.0 on the 1st and 15th days, respectively; size and PdI less than 250 nm and 0.35, respectively, negative zeta potential, showing good stability for the LN. Thus, the nanoparticle composition influenced the physicochemical features and the developed method was effective to produce LN containing vegetal butters to be topically applied to promote skin hydration. Financial support or fellowship: State University of Ponta Grossa. Acknowledgement for NTA (José Orestes Del Ciampo, FCFRP-USP)

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

LIPID-BIOPOLYMERIC MICROSPHERES FOR INDOMETHACIN SUSTAINED RELEASE

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CFSP287

FLÁVIA MONIQUE ROCHA BONETTI; ENEIDA DE PAULA; LIGIA NUNES DE MORAIS RIBEIRO. Unicamp, Campinas - SP - Brazil. Introduction and objective: Improve the efficiency and decrease the side effects of drugs. Hybrid DDS, which is based on different nature of excipients, combines the advantages of each material in a single biodevice. Objectives: We propose a novel lipid-biopolymeric nanostructured microsphere DDS. Indomethacin (INDO) is an anti-inflammatory used to reduce the symptoms of inflammatory disorders. The oral administration is the current route, which causes several side systemic effects. Drug delivery systems (DDS) provide formulations of nanostructured lipid carrier (NLC) encapsulating INDO, plus chitosan and xanthan matrices processing the gastroresistant and mucoadhesive microspheres. Materials and methods: The INDO-loaded NLC (myristyl miristate, coconut oil, Pluronic 68®, and 2% INDO) was prepared by the emulsification-ultrasound method, which was blended with chitosan (2%) providing a homogenous system that was crosslinked (tripolyphosphate solution) forming the hybrid microspheres. This system was then coated with a xanthan (0.5%). The microsphere size and dispersion was calculated by ImageJ software; the in vitro microsphere water absorption (for 6 h) as well as the in vitro INDO release (for 26 h) studies were carried out simulating the oral route. Results and conclusion: The lipid-biopolymeric microspheres were successfully prepared; exhibiting sizes around 0.3 cm and a monodisperse distribution. Such elegant microspheres presented excellent in vitro swelling properties and a prolonged in vitro INDO release profile up to 26 h. The synergism between the lipid and biopolymeric compounds prevented the burst release effect in the first 2 h of experiments simulating the gastric medium, followed by a prolonged release in the basic condition that mimicked the intestinal medium. The xanthan coating also protected the solubilization of chitosan in the acidic condition, which was able to act as mucoadhesive excipient in the intestinal mucosa. This system appears as an excellent candidate to deliver indomethacin, as well as other anti-inflammatories, through the oral route. Financial support or fellowship: FAPESP (#2014/25372-0; #2014/14457-5). CFSP288 LIPIDOMIC PROFILING IN PLASMA OF HUMAN RECTAL CANCER USING HIGH-RESOLUTION MASS SPECTROMETRY (ESI-q-TOF) AND GAS CHROMATOGRAPHY (GC) MÁRCIA CRISTINA FERNANDES MESSIAS1; CÉLIO FERNANDO FIGUEIREDO ANGOLINI2; CARLOS AUGUSTO REAL MARTINEZ3; GIOVANA COLOZZA MECATTI3; LAURA CREDIDIO2; ALINE GONÇALVES MORA4; TIAGO TOMAZINI GONÇALVES4; GIAN LUCAS MELO ARRUDA3; PATRÍCIA DE OLIVEIRA CARVALHO5. Universidade São Francisco, Bragança Paulista - SP - Brazil; 2Universidade de Campinas, Campinas - SP - Brazil; 3Universidade Sao Francisco, Braganca Paulista - SP - Brazil; 4Universidade Sao Francisco, Bragança Paulista - SP - Brazil; 5Universidade Sao Francisco, Itatiba - SP - Brazil. 1

Introduction and objective: Rectal cancer is one of the most common malignancies in the gastrointestinal tract, with a high incidence and high mortality. The objective of this study was to evaluate the biomarkers of lipid profile in the plasma of patients with rectal cancer in order to identify the differential metabolites and contribute to the knowledge of the alterations in lipid metabolism involved in carcinogenesis. Materials and methods: Blood samples from 23 patients with rectal cancer stratified according to the stage and 18 healthy volunteers were used. The lipid profile was evaluated by gas chromatography (GC) and high resolution mass spectrometry (ESIq-TOF) associated with a multivariate statistical technique. The Variable Importance in Projection (VIP) was used to identify the ions that had the greatest discriminatory effect between the groups. Results and conclusion: The results showed that several lipid metabolites were altered in the plasma of patients with rectal cancer. The most abundant ions identified in this group of patients were phosphatidylcholine and phosphatidylethanolamine while those of lisophosphatidylcholine (LPC), identified as LPC (16:1), LPC (18:1) and LPC (18:2), were down-regulated. It was found that a LPC plasmalogen containing palmitoleic acid (LPC (P-16:1)), with the highest variable importance projection (VIP) score, showed a low tendency in the cancer patients. There was also a reduction of polyunsaturated fatty acids (PUFA), especially arachidonic (C20:4) acid and n-3 PUFA. In this study, it was possible to evaluate the importance of the use of the lipidomic strategy as a tool to identify new potential biomarkers mediators of cancer. Financial support or fellowship: FAPESP; CAPES

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CFSP289 LIQUID CRYSTALLINE SYSTEMS BASED ON BRIJ®97/WATER FOR SKIN DELIVERY OF METHYLENE BLUE: RELEASE AND RETENTION STUDIES THALITA PEDRALINO GONÇALVES; ÉRICKA SÃO FELIX MARTINS; MARIA TERESA JUNQUEIRA GARCIA. Universidade Federal de São Paulo, Diadema - SP - Brazil. Introduction and objective: The stratum corneum constitutes a barrier to skin delivery of drugs. The development of a system for topical delivery of methylene blue (MB) is a strategy in the optimization of photodynamic therapy (PDT) for the treatment of skin cancer. Liquid crystalline (LC) phases have demonstrated potential to improve cutaneous delivery of drug. The aim of this work was to characterize the release and skin retention of MB in LC phases based on Brij®97/water. Materials and methods: Brij®97 and water were mixed at proportion 8:2 and 6:4. MB (0.1%,w/w) was added to LC phases. In vitro release and penetration studies of MB were carried out in adapted diffusion cells using cellulose acetate and porcine ear skin as membranes, respectively; and 5.0%(w/w) hydroxyethylcellulose (HEC) gel and phosphate buffer (pH7.4) were used as receptor phase, respectively. Formulations were placed in the donor compartment. After 6 hours, MB was extracted from HEC gel or skin and assayed by UV/Vis spectrophotometer (662 nm). MB (0.1%,w/w) in HEC gel (3.0%,w/w) was used as control formulation. Results and conclusion: The associations of the Brij®97 and water resulted in lamellar (L) (8:2) and hexagonal (H) (6:4) phases. The skin retentions of the MB in 6 hours from the L phase, H phase and control were about 52.8(±5.2), 33.1(±4.7) and 24.9(±2.7) µg/cm2, respectively. The amount of the MB released in 6 hours from the L phase, H phase and control was about 13%, 9% and 42%, respectively. The MB skin retentions from the L and H phases were about 2.1(p<0.001) and 1.3(p><0.05) fold higher than control, respectively. The MB skin retention from the L phase was 1.6-fold higher than the H phase (p><0.001). Higher MB release did not necessarily increase MB skin retention. The higher MB skin retention from the L and H phases can be attributed to the effect of the Brij®97 as enhancing penetration, which modifies skin properties, improving drug penetration. A higher MB delivery from L phase may have contributed to higher skin retention of the MB as compared to the H phase. The results suggest that the L and H phases based on Brij®97/water can be explored to prolong and enhance the MB retention for PDT of skin cancer.

MESALAMINE POLYMERIC CONJUGATE: PREPARATION AND CHARACTERIZATION

CFSP290

AINA LIZ ALVES CESAR; LARISSA CALDEIRA NAVARRO; RACHEL OLIVEIRA CASTILHO; SIMONE ODILIA ANTUNES FERNANDES; VALBERT NASCIMENTO CARDOSO; FERNANDA ALVES ABRANTES; ANDRE AUGUSTO GOMES FARACO. UFMG, Belo Horizonte - MG - Brazil. Introduction and objective: Mesalamine (5-ASA) is the first line treatment for mild to moderate ulcerative colitis. The daily dosage is elevated due to the high absorption in the upper gastrointestinal tract resulting in low bioavailability and several adverse systemic effects. Polymeric prodrugs (polymeric conjugate), can overcome these drawbacks, as they act as targeting agents to the site of action. Chondroitin sulfate (CS) is an interesting polymer as it is biodegradable and has mucoadhesive characteristics. The purpose of this work was to synthesize and characterize the conjugate CS-5-ASA and evaluate its dissolution profile. Materials and methods: The synthesis of CS-5-ASA was performed via Steglich esterification. The system was characterized by 1H NMR and the dissolution profile was conducted under different pH conditions. Results and conclusion: The 1H NMR spectrum revealed signals at the aromatic region (δ 7.59 – 6.68 ppm) that were attributed to the presence of 5-ASA due to the correspondence with tabulate values for chemical shifts of aromatic ring substituted in ortho, meta and para positions. This spectrum was different from that obtained by the physical mixture of reactants, indicating the formation of a new chemical entity. The drug delivery profile at different pH values indicates a pH dependent release for up 50 hours. The prolonged release corroborates with the hypothesis of the covalent linkage between the drug and polymer. Besides that, the conjugate was more stable in acid medium, suggesting that CS-5-ASA will pass intact through the small intestine, which is in agreement with the objective. The polymeric conjugate CS-5-ASA consisting of 5-ASA linked to a CS chain was successfully synthesized via EDC coupling reagent. Thus, after additional in vivo studies, the CS-5-ASA conjugate will be able to be considered as an alternative treatment for ulcerative colitis. Financial support or fellowship: Financial Support: Fapemig; CNPq; CAPES.

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CFSP291 METHOD FOR SIMULTANEOUS ASSAY OF ADIPHENINE CHLORIDRATE, PROMETHAZINE CHLORIDRATE AND DIPYRONE BY HPLC-DAD AND UHPSFC-DAD PAULA ROBERTA CAUMO DE CAMARGO; PAULO ROSA; MARCIA BREITKREITZ; LUCÍLIA MELO; ISABEL JARDIM. Unicamp, Sumaré - SP – Brazil. Introduction and objective: The combination of drugs adiphenine, promethazine and dipyrone is indicated for post operative pain and headache. The official chromatographic methods available for analysis are for the isolated drugs and there is no exclusive method for their simultaneous quantification. Objective: To develop a chromatographic method for simultaneous assay of adiphenine, promethazine and dipyrone drugs using High Performance Liquid Chromatography (HPLC) and Ultra High Efficiency Supercritical Fluid Chromatography (UHPSFC). Materials and methods: For HPLC analysis the following conditions were used: methanol diluent, C18 column, 1mL.min-1 flow, mobile phase phosphate buffer pH 3,5:Metanol:Acetonitrila. For UHPSFC: ethanol diluent, C18 column, 1.5mL.min-1 flow, mobile phase CO2: Methanol (with the addition of 0,2% triethylamine and 5% water). Results and conclusion: A single method was developed for simultaneous dosing of drugs. By HPLC, the analysis time was 25 minutes, for UHPSFC, was 2.5 minutes, both with resolution values higher than 1.5. UHPSFC was advantageous in relation to HPLC due to its short analysis time, low solvent and sample consumption, and lower residues generation. Financial support or fellowship: CAPES CFSP292 MYRCIA SPECTABILIS DC. LEAF ETHANOLIC EXTRACT OPTIMIZATION USING FACTORIAL DESIGN JOSÉ ARMANDO JÚNIOR1; JAQUELINE OLIVEIRA DOS SANTOS1; MARA ANGELINA GALVÃO MAGENTA2; ROBSON MIRANDA DA GAMA1. FMABC, Santo André - SP - Brazil; 2Unisanta, Santos - SP - Brazil.

Introduction and objective: Myrcia spectabilis DC. is a plant belonging to the Myrtaceae family, genus widely used as food and in folk medicine because of anti-inflammatory, antimicrobial, antioxidant and healing properties. The use of statistical methods as factorial design allows the relationship between several factors that influence the extraction of active substance of plants to be evaluated to obtain the best conditions of the plant extract with a higher content of active substances for use in medicines or cosmetics. This work aims to study how the factors of maceration process extraction interfere in the characteristics and content of marker substances such as tannins, total phenol and flavonoids and antioxidant activity (AA) using full factorial design 25 obtained for Myrcia spectabilis DC. dried leaves. Materials and methods: In this study, five factors considered important in the process were evaluated, selected in two levels: drug/solvent ratio (10:100 and 50:100) extraction solvent (70% ethanol and absolute ethanol), time (6h and 24h), pH (3 and 12) and mixing time (0 and 24 h). The experiments were performed according to factorial design matrix 25 using the Minitab® software, resulting in 32 trials. The responses evaluated were: total tannins and total phenol content of the extracts was measured by the method for precipitation assays; total flavonoids content of the extracts was measured by aluminum chloride colorimetric method and antioxidant activity by the DPPH (2,2-diphenyl-1-picryl-hydrazyl) method. Results and conclusion: The factors that most influenced the extraction process were: absolute ethanol for phenols and flavonoids and 70% for AA; interaction between solvent and drug/solvent ratio (50:100) for phenols, flavonoids, tannin and AA; pH of the solvent at 3 for all assays except tannins, 24 h mixing time for phenols and flavonoids and without mixing time for AA. Financial support or fellowship: FMABC.

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CFSP293 NANOSTRUCTURED POLYMERS TO DELIVER ANTIMICROBIAL PEPTIDES WITH LEISHMANICIDAL ACTIVITY JULIANA SOUZA RIBEIRO COSTA1; MARÍLIA MEDEIROS2; GUILHERME DINIZ TAVARES3; EDITE HATSUMI YAMASHIRO-KANASHIRO4; MUSSYA CISOTTO ROCHA4; PAULO CÉSAR COTRIM4; LAURA DE OLIVEIRA NASCIMENTO5. Department of Biochemistry and Tissue Biology, Biology Institute, University of Campinas, Campinas - SP - Brazil; 2Faculty of Pharmaceutical Sciences, University of Campinas, Campinas - SP - Brazil; 3Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora - MG - Brazil; 4Institute of Tropical Medicine, University of São Paulo, São Paulo - SP - Brazil; 5Dbtb - Ib/ Unicamp and Faculty of Pharmaceutical Sciences, University of Campinas, Campinas - SP - Brazil. 1

Introduction and objective: Leishmaniasis is caused by protozoans of the genus Leishmania. The drugs available for treatment of leishmaniasis are highly toxic, require hospitalization and prolonged infusions. Therefore, poly n-butyl cyanoacrylate (PBCA) nanoparticles were produced for subsequent incorporation of polymyxin B, an antimicrobial peptide with a potential leishmanicidal action. Materials and methods: PBCA nanoparticles (PBCAnp) were formulated by anionic polymerization. Therefore, formulated nanoparticles were characterized by their size, ζ potential, encapsulation efficiency, release profile, and morphology, as well as evaluation of leishmanicidal and cytotoxic activity of the formulations. Results and conclusion: PBCAnp had an average size of 216.9 nm, which did not change significantly with the addition of polymyxin B. The average ζ potential becomes negative (the empty nanoparticles samples) to positive (nanoparticles with polymyxin B). The encapsulation efficiency was inversely proportional to the increase of polymyxin B. The release profile showed a sustained release of polymyxin B when incorporated in nanoparticles, adequate for internalization by infected macrophages. The IC50 found for the polymyxin B free was 55 mM. The PBCAnp p5 formulation reached IC50 3.7 µM (nanoparticle dilution = 1: 980). The empty nanoparticle also presented leishmanicidal activity (dilution of the nanoparticle in IC50 = 1: 659), accounted in large part for the polymyxin B IC50 decrease. All formulations showed cytotoxic activity in macrophages in the IC50 concentrations for extracellular promastigotes of Leishmania amazonensis. The PBCAnps with polymyxin B proved inadequate as leishmanicidal but adequate as a bactericide or as functionalized carrier for other drugs with high IC50. Financial support or fellowship: FAPESP; FAEPEX/UNICAMP; CAPES CFSP294 OPTIMIZATION BY FACTORIAL DESIGN OF BUPIVACAINE LOADED NANOSTRUCTURED LIPID CARRIERS PREPARED WITH BEESWAX AND MELALEUCA OIL GABRIELA GERONIMO1; GUSTAVO HENRIQUE RODRIGUES DA SILVA2; LIGIA NUNES DE MORAIS RIBEIRO2; SIMONE RAMOS DE CASTRO2; MÁRCIA CRISTINA BREITKREITZ2; ENEIDA DE PAULA2. Unicamp, Campinas - Brazil; 2Unicamp, Campinas - SP - Brazil.

Introduction and objective: The control of pain in burn injuries and their treatment remains a challenge. Local anesthetics, as such bupivacaine (BVC), are potential drugs to control burn pain, but they present limitations such as short time of action and toxicity. This study, using factorial design (FD), aimed to optimize a formulation of Nanostructured Lipid Carriers (NLC) functionalized with beeswax (solid lipid - SL) and melaleuca oil (liquid lipid - LL) with 1% (w:w) of BVC. Materials and methods: A 2³ FD with triplicate on central point was conducted by varying the SL/LL proportion (from 90:10 to 70:30 %), the surfactant concentration (SC = 2.5 to 5 % w:w), and the concentration of lipids (LC = 5 to 15 % w:w). The observed responses were: hydrodynamic ratio (particles size), polydispersity index (PDI) and Zeta potential, having as the optimized formulation a minimal particle size and PDI values and maximum Zeta potential, in modulus. Results and conclusion: The results showed that SC is inversely related to the particles size, while LC directly affect the NLC diameters. For the PDI response, both LS/LL and LC were found to decrease it. LS/LL negatively influenced the Zeta potential values while LC displayed a positive effect. All models generated for the responses were significant (p<0.05) and without lack of fit. The optimized formulation was composed of 70:30 % LS/LL, 15% LC and 5% SC (w:w), with 252.2 ±2.0 nm size, PDI = 0.154 ±0.011 and Zeta potential = -48.6 ± 1.2 mV. In conclusion, the factorial design approach was able to identify the relevant factors for the desired formulation responses and to determine the best formulation for the upcoming characterization tests. Financial support or fellowship: FAPESP (#14/14457-5 grant); CAPES.

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CFSP295 PEPTIDES FROM SCORPION VENOM AS PHARMACOLOGICAL TOOLS: THE OTHER SIDE OF THE COIN BRUNO DUZZI1; DANIELA CAJADO-CARVALHO1; ALEXANDRE KUNIYOSHI1; ROBERTO KODAMA1; FABIO CESAR GOZZO2; MARIANA FIORAMONTE2; GISELE PICOLO3; VANESSA RIOLI4; FERNANDA PORTARO1. Immunochemistry Laboratory, Butantan Institute, São Paulo - SP - Brazil; 2Chemistry Institute, University Of Campinas, Campinas - SP - Brazil; 3Special Laboratory of Pain and Signalization, Butantan Institute, São Paulo - SP - Brazil; 4Special Laboratory of Applied Toxinology, Butantan Institute, São Paulo, Sp, Brazil, São Paulo - SP - Brazil. 1

Introduction and objective: Fear! The most commonly word used by the human being to designate venomous animals. One example is the Tityus serrulatus scorpion (Brazilian yellow scorpion), recognized by the severe manifestations in accidents, being the most dangerous specie in Brazil. Although the crude venom causes severe local and systemic manifestations, some components, such as peptides, when isolated and characterized, can be used as pharmacological tools. An example is captopril, designed from B. jararaca venom peptide. We aimed to identify molecules that could be used as pharmacological tools in pain and/or blood pressure control. Materials and methods: Lyophilized venom was fractionated using a 3kDa membrane and HPLC. Fluorimetric assays were used to select fractions that could inhibit the neurolysin and neprilysin enzymes activities, two enzymes of medical importance. The peptide sequences were determined by mass spectrometry. Synthetic peptides were tested as neprilysin inhibitors and in pain control model on Wistar rats by Randall - Selitto assay. Results and conclusion: Two peptides were selected and synthetized. The first one is a NEP inhibitor with Ki of 0.94 µM, being a good inhibitor of this enzyme which may be used as a model to antihypertensive drug development. The second one is capable of inducing antinociceptive response after local injection in Wistar rats’ paws. This antinociceptive effect was reverted by naloxone, an opioid antagonist. With this work, we try to demonstrate that animal venom can be used as a rich source of pharmacological tools for new drug development in the future. Financial support or fellowship: FAPESP; CAPES; CNPq. CFSP296 PHARMACOGNOTIC ANALYSIS: RESEARCH OF MICROBIOLOGICAL CONTAMINANTS IN HERBAL DRUGS ORIGINATED FROM COMMERCIAL SAMPLES OF MATRICARIA CHAMOMILLA L. VITHORUGO S. BETTINI; ADILSON PAGANINI; FLAVIA DE MELLO SANTOS FRANCO DE PAULA; ADEMIR SALVI JÚNIOR. Centro Regional Universitário de Espírito Santo do Pinhal - UNIPinhal, Esp. Sto. do Pinhal - SP - Brazil. Introduction and objective: Matricaria chamomilla L., belonging to the family Asteraceae, popularly known as chamomile, native of Europe. Inflorescences are used in therapy because of their antispasmodic, anxiolytic and sedative action (internal use) and anti-inflammatory in affections of the oral cavity (external use). The objective was to evaluate the quality, as regards microbiological contaminants, of the plant drug M. chamomilla, of commercial origin. Materials and methods: Three samples of M. chamomilla marketed in bulk in natural products stores, were purchased in AndradasMG and identified as sample A, B and C, for testing purposes. The preparation of the samples was carried out according to norms of the Brazilian Pharmacopoeia, adapting the techniques proposed and, posteriorly, submitted to the test of counting the total number of mesophilic microorganisms and research of pathogenic microorganisms, as described in the Brazilian Pharmacopoeia (2010, p.237-242). Results and conclusion: The results of the tests, referred to by the average of three determinations, revealed absence of Escherichia coli and Salmonella spp.; a number of total aerobic microorganisms of 2.18x104, 2.15x104 and 2.91x104 UFC g-1, and mold and yeast of 3.07x104, 2.25x104 and 1.94x104 UFC g-1; the most likely number of bile-tolerant gram-negative bacteria per gram of product was less than 103 and more than 102, less than 103 and more than 102 and less than 102 and more than 10, for samples A, B and C, respectively. According to the limits established by the Brazilian Pharmacopoeia (2010, p.249), the total count of fungi and yeasts presented values above the acceptable limit, which reveals that the quality of the samples is compromised, and their commercialization, without the expected quality. It is a matter of concern, implying health risks, since they are used by a large part of the population, as a therapeutic resource. Financial support or fellowship: Centro Regional Universitário de Espírito Santo do Pinhal - UniPinhal

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PHOTOSTABILITY STUDY OF LIPID EMULSION FORMULATIONS FOR PARENTERAL NUTRITION

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CFSP297

REBECA RODRIGUES ARRUDA GOMES1; JOÃO PAULO DE MÉLO GUEDES1; JULIANA DE SOUZA ALENCARA FALCÃO2; CLAYTON ANDERSON DE AZEVEDO FILHO3; BEATE SAEGESSER SANTOS1. Universidade Federal de Pernambuco, Recife - PE - Brazil; 2Universidade Federal de Campina Grande, Recife - PE - Brazil; Faculdade Asces, Caruaru - PE - Brazil.

1 3

Introduction and objective: Parenteral nutrition (PN) are sterile emulsions composed of carbohydrates, amino acids, lipids, vitamins and minerals, applied to endovenous administration. Objectives: The aim of this work was to analyze lipid emulsion (LE) photodegradation when they are mixed with the other components in PN pediatric formulations, and furthermore, evaluate a possible antioxidant action of sodium polyphosphate ([Na(PO3)12]) on the LE. Materials and methods: The PN formulations were photodegraded in a photobleaching chamber through different testing doses in the ultraviolet (UV) and visible (VIS) region, equivalent to 24 and 48 h of hospital light environment. A statistic factorial design was performed to evaluate the photoprotective effect of sodium polyphosphate (food antioxidant) in these systems in the presence of a photosensitizing substance (Riboflavin) and a potent oxidant: iron sulfate. The characterization of the photodegraded lipidic components was performed by applying high resolution gas chromatography by flame ionization detection (GC-FID), vibrational infrared (IR) and Raman spectroscopies. Results and conclusion: Analysis of the chromatographic and vibrational data showed that Na(PO3)12 had a small protective effect for linoleic acid and no effect on linolenic acid (major components of LE). LE photodegraded in the presence of Fe2+ ions showed differences in the IR and Raman spectra showing that these techniques are effective in the identification of photooxidative processes in lipids. Financial support or fellowship: CNPq CFSP298 PHYSICAL CHARACTERIZATION OF THE MICROPARTICULATED SYSTEM BNZ: ZIF-8 USED TO OPTIMIZE THE TREATMENT OF CHAGAS’ DISEASE ALINE SILVA FERREIRA1; ALINNE ÉLIDA GONÇALVES ALVES2; LESLIE RAPHAEL DE MOURA FERRAZ2; DÉBORA DOLORES SOUZA DA SILVA2; ALESSANDRA CRISTINA SILVA1; ROSALI MARIA FERREIRA DA SILVA2; LARISSA ARAÚJO ROLIM3; PEDRO JOSÉ ROLIM NETO2. Centro Universitário Maurício de Nassau, Recife - PE - Brazil; 2Universidade Federal de Pernambuco, Recife - PE - Brazil; Universidade Federal do Vale do São Francisco, Petrolina - PE - Brazil.

1 3

Introduction and objective: Chagas’ disease is a neglected disease that affects around eight million people, being caused by the protozoan Trypanosoma cruzi. Benznidazole, chemically (N-benzyl-2-nitro-1-imidazoleacetamide) (BNZ), is a drug with high antiparasitic pharmacological activity, but low solubility in water and high toxicity. From the technological barriers presented, it is necessary to develop new pharmaceutical excipients capable of promoting an increase in the dissolution rate of the drug, in addition to a prolonged and vectored release and, consequently, to improve its bioavailability and reduce the side effects presented. Belonging to a subgroup of MOF’s, ZIF-8 have attractive structural properties and great potential for chemical storage, encapsulation and controlled delivery, in addition to presenting another very important characteristic, the low toxicity. Therefore, the objective of the work was to perform the physical characterization of the BNZ, ZIF-8, the physical mixture (for comparison) and the BNZ:ZIF-8 systems through various analytical techniques.”; Materials and methods: Infrared analysis, X-ray diffraction, Scanning Electron Microscopy, Analysis of the particle size, surface area analysis, pore size and volume were the techniques used to characterize the materials. Results and conclusion: By means of the analyzes, it was possible to observe a reduction in the size of the BNZ crystal when incorporated into ZIF-8, corroborating to the formation of the microparticulate system with consequent increase of the surface area, leading to the higher degree of the drug crystal solvation , which suggests an increase in dissolution rate. Financial support or fellowship: CAPES; CNPq; FACEPE

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CFSP299 PHYSICAL EVALUATION OF INDUSTRIALIZED TABLETS OF DIPYRONE 1G: COMPARATIVE ANALYSIS BETWEEN REFERENCE, GENERIC AND SIMILAR MEDICINES ADNA VASCONCELOS FONTELES1; ANTONIA RAFAELLA FERREIRA GOMES MARTINS2; BRENA GEYSE MESQUITA ROCHA SOARES3; ANTONIO ERIVELTON PASSOS FONTENELE4; IANNE DE FATIMA SAMPAIO MARINHO2; ARISTIDES AVILO DO NASCIMENTO5. Santa Casa de Misericórdia de Sobral/Inta, Sobral - Brazil; 2Santa Casa de Misericórdia de Sobral/Inta, Sobral - CE - Brasil; Santa Casa de Misericórdia de Sobral/Inta, Sobbral - CE - Brasil; 4Santa Casa de Misericórdia de Sobral, - ; 5Instituto Superior de Teologia Aplicada - Inta, Sobral - CE - Brazil. 1 3

Introduction and objective: Dipyrone is marketed by several manufacturers and it is possible to find, in addition to referring medicines, generic and similar products. In view of this, the quality control of the various versions is extremely important for the prevention of compounds between manufacturers or between batches, which are generated by therapeutic effects. The present work has the objective of analyzing the physicists of reference, generic and similar drugs of active Dipyrone in concentration 1g. Materials and methods: This is a comparative and qualitative-quantitative experimental study, where a physical-chemical evaluation of the Dipyrone 1g tablets manufactured from three different laboratories, generic, and similar, were performed in triplicate with different batches denominated R1, R2 and R3 (Reference), G1, G2 and G3 (Generic) and S1, S2, and S3 (Similar). The destructive tests (friability and hardness), the non-destructive tests (average weight and visual aspects), and statistical analyses (standard deviation and coefficient of variation) were performed. Results and conclusion: Mean weight tests were within the established mean, the lowest mean weight was 1.0525g of the R1 group and the highest was G1 (1.2084g). In the friability test, all groups were below 1.5%. In the hardness test, a high standard deviation was observed. However, all parameters were within the values accepted by the Brazilian Pharmacopoeia 5 edition. Considering this, within the parameters analyzed, the generic and similar medicine were equivalent to the reference. Financial support or fellowship: SANTA CASA DE MISERICÓRDIA DE SOBRAL; INTA CFSP300 PHYSICAL QUALITY CONTROL OF DIPYRONE TABLETS: REFERENCE, GENERIC AND SIMILAR COMPARATIVE STUDY JAMYLYS FIDELIS DA SILVA; GUSTAVO DE OLIVEIRA ALENCAR; CAMILA ALENCAR BEZERRA; JEAN PIERRE DE OLIVEIRA ALENCAR; MARIA DE FÁTIMA ROCHA BARRETO; RAFAEL DE CARVALHO MENDES. Faculdade Juazeiro do Norte, Juazeiro do Norte - CE - Brazil. Introduction and objective: Dipyrone has analgesic and antipyretic actions. It is presented in several pharmaceutical forms: oral solution, injectable, tablets and suppositories. The main purpose of the present study is to evaluate the physical quality of the brands of commercially available dipyrone 500 mg tablets. Including: generic, reference and similar; available from commercial pharmacies in Juazeiro do Norte - CE. Materials and methods: This is a quantitative, comparative and experimental study, carried out in October 2016. All tests followed the Brazilian Pharmacopoeia 5th edition and were studied at the Laboratory of the Faculty of Juazeiro do Norte (FJN), among the tests analyzed were: average weight, hardness, friability and disintegration. Results and conclusion: In the mean weight test all tablets were within the range of ± 5% following the average weight of each sample. In the mechanical resistance, such as hardness and friability, the results were sample A (115.3 N/0.431%), sample B (67.25 N/0.257%), sample C (104.25 N/0.098%). The disintegration time of the tablets all showed below the 30 minute limit. It was observed that the studied samples answered the question of the safety, efficacy and quality desired, showing the good practices of manufacture of medicines by the industries in the matter of physical analysis of the drug. Financial support or fellowship: FJN

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CFSP301 PHYSICAL-CHEMICAL AND MICROBIOLOGICAL STABILITY EVALUATION OF SUNSCREENS PRODUCED IN COMPOUNDING PHARMACIES. FLAVIA SCIGLIANO DABBUR; JOSEFA RENALVA DE MACEDO COSTA; FERNANDA SOARES CARVALHO; KATHYANY SANDRYELY DE FARIAS; JOANA ÍRIS DA SILVA BARBOSA; NAYANNA KELLY ALVES PORTO. Centro Universitário Cesmac, Maceio - AL - Brazil. Introduction and objective: The use of sunscreen has been a very effective alternative to avoid skin cancer and hyperchromias. Customized sunscreen can add properties according to the customer’s need and exclude any substance that causes discomfort. The purpose of this work was to evaluate the physicochemical and microbiological stability of sunscreens produced in compounding Pharmacies. Materials and methods: The preparation of 500 mL sunscreen emulsion, with 30 SPF, was requested from seven Maceio City Pharmacies (only Companies registered in the Regional Pharmacy Council). They were acquired by direct purchase in the period from August to September 2016. The stability evaluation was performed according to the Cosmetics Stability Guide of the Brazilian National Sanitary Agency and samples were kept for 180 days at temperatures 5° C, 25° C and 45° C. The physical-chemical tests were organoleptic characteristics, pH, spreadability and centrifugation. The “in vitro” sensory analyses were sliding, white residue and stickiness. The microbiological count was performed according to the Brazilian Pharmacopoeia 5th edition. The sample values were checked for each 100 mL. Results and conclusion: Only sample number one presented physical instability from the first analysis and another four samples (number 4, 5, 6 and 7) were unstable only after 180 days at 45 ºC. The organoleptic characteristics did not significantly change in any of the samples, the pH ranged from 5.5 to 7.5, the sensorial analyzes had variations between samples because the formulations had different ingredients, in the microbial counting only samples number 5 and 6 were above the allowed, the variation from the lowest to the highest value was 33 %. The conclusion is that some pharmacies need to improve their formulations to meet the specifications. Financial support or fellowship: Programa Semente de Iniciação Científica - Cesmac CFSP302 PHYSICAL-CHEMICAL QUALITY CONTROL IN COPAÍBA OLEORESIN SOLD AT THE VER-O-PESO MARKET WEILLA PATRÍCIA CORDEIRO SILVA; INGRID SILVA DE OLIVEIRA¹; JOIANE PAIVA TEIXEIRA; AFONSO RAFAEL NASCIMENTO E SILVA¹; DANILA TERESA VALERIANO ALVES¹; CHRISTIAN NERI LAMEIRA¹. Faculdade Integrada Brasil Amazônia, Belém - PA - Brazil. Introduction and objective: Oleoresin is widely used in popular medicine and despite all associated activities, this product is subject to adequate quality control. Objective: Perform quality control in the copaiba oleoresin sold in the market of Ver-o-Peso Materials and methods: Eighteen samples were analyzed. The coloring analysis was performed according to the recommended methodology where they are classified as colorless, light yellow, medium yellow, golden yellow and brownish. The pH was determined using a pH meter calibrated with buffer solution of pH 4 and 7. For the acidity test, 2 g of oleoresin with 25 mL of the ethyl ether/ethyl alcohol (2:1) was used accompanied by shaking. Then, 2 drops of the phenolphthalein indicator was added and titrated with 0.1 N NaOH solution to the first appearance of a permanent pink color. The density analysis was realized at room temperature and the samples were weighed in 10 mL pycnometers. The procedure was performed in triplicate, obtaining an average value of density Results and conclusion: The color of the oleoresin analyzed was 72.5% light yellow and 27.5% yellow-gold. The pH of the control was 5.62 similar to that described in the literature only two samples were outside of the established standard. In acidity analysis index, comparison with the control suggested that some samples were adulterated with ethyl alcohol or mineral oil and others adulterated with fatty oils. The analysis of the density revealed that some samples showed values that were discrepant with the control, indicating that there is a possibility of adulteration of the samples by alcohol or vegetable oil. However, more studies should be performed to confirm adulteration of oleoresin Financial support or fellowship: Faculdade Integrada Brasil Amazônia

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CFSP303 PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY IN VITRO OF AQUEOUS EXTRACT OBTAINED FROM THE MESOCARP (FIBROUS HUSK) OF COCUS NUCIFERA L.: A POTENTIAL FUTURE TREATMENT OF GASTRIC ULCERS MATHEUS ROBERTI DE CARVALHO; WALBER TOMA* *Centro Universitário São Camilo, São Paulo - SP - Brazil. Introduction and objective: Cocus nucifera L., commonly called the Coco, Coco-da-Bahia and Coco-Verde, is the most naturally widespread fruit plant on Earth (LIMA et al., 2015). Ethnopharmacological information showed that Cocus nucifera mesocarp (fibrous husk) is used to treat stomachaches (HOLDSWORTH, 1992). Despite this information, on the Brazilian coast Cocus nucifera are considered a residue problem caused by improper disposal. The objective of this work was to obtain the aqueous extract of fibrous husk discarded in the Santos-SP Brazilian Coast and perform phytochemical analysis and antioxidant activity in vitro of this extract. Materials and methods: The fibrous husk of Coco nuts were obtained from Santos-SP Brazilian Coast and submitted to the decoction extraction. For the evaluation of the presence of the phenolic compounds, the aqueous extract (AE) was submitted to the Shinoda and Aluminum Hydroxide reactions. To screen the antioxidant capacity of (AE), a Thin Layer Chromatography Bioautography (TLC-B) method was performed. After separation on TLC plates, the compounds with radical scavenging activity were determined in situ with DPPH reagent. Results and conclusion: The Shinoda and Aluminum reactions showed a positive results and indicated the presence of phenolic compounds. The (TCL-B) showed the presence of two compounds (Rf = 0.23 and 0.40) with antioxidant activity. These results demonstrated that the antioxidant activity of (AE) can be responsible for the treatment of stomachaches and showed a perspective in the future for the treatment of gastric ulcers. Financial support or fellowship: Centro Universitário São Camilo CFSP304 PHYTOCHEMICAL PROFILE AND VASODILATORY AND ANTIOXIDANT ACTIVITIES OF ETHANOL EXTRACT AND FRACTIONS FROM EUGENIA UMBELIFLORA LEAVES THALISSA COZENTINO DE ALBUQUERQUE; SHAFT CORREA PINTO; JULIANA MONTANI RAIMUNDO. Universidade Federal do Rio de Janeiro - Campus Macaé, Macaé - RJ - Brazil. Introduction and objective: Eugenia Umbeliflora (Myrtaceae), popularly known as guapé, is used in folk medicine for the treatment of diseases such as diabetes, arthritis and rheumatism. The objectives of this work were to evaluate the chemical profile, as well as the antioxidant and vasodilatory activities of Eugenia Umbeliflora leaves. Materials and methods: The liquid-liquid partitioning of the ethanol extract of Eugenia Umbeliflora leaves was performed and the phytochemical profile of the fractions was evaluated by high performance liquid chromatography coupled to diode arrangement detector (HPLC-DAD). The antioxidant activity of the ethanol extract and its fractions (1.4 to 140 μg/mL) was evaluated through the DPPH assay. Aortic rings from male Wistar rats (220–280 g) were prepared for isometric tension recording. The contractile response to phenylephrine (10 μM) was measured before and after exposure to increasing concentrations of the extract or fractions (1-300 μg/mL). The local Animal Care and Use Committee approved all protocols (License MAC019). Results and conclusion: At 10 μg/mL, the ethanol extract induced vasodilation of 65.1 ± 4.8 % (P <0.05), which was completely inhibited in aortas without endothelium. Butanol and dichloromethane fractions induced vascular relaxation of 57.3 ± 11.6 % and 19.9 ± 7.3 %, respectively, at 30 μg/mL. Ethyl acetate 1 (EC50 8.5 ± 0.5 μg/mL) and 2 (EC50 3.4 ± 1.1 μg/mL) and butanol fractions (EC50 6.5 ± 0.6 μg/mL) presented higher antioxidant potency than the ethanol extract (EC50 10.5 ± 0.8 μg/mL). The hexane and dichloromethane fractions were less potent. Phytochemical profile of ethyl acetate 1 and 2 and butanol fractions obtained by HPLC-DAD showed, mainly, the presence of flavonoids. The vasodilatory and antioxidant activities of the butanol fraction may be related to the presence of flavonoids. Financial support or fellowship: PIBIC, FAPERJ, CNPq.

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CFSP305 PHYTOCHEMICAL PROSPECTION AND EVALUATION OF CYTOTOXICITY OF CRUD AQUEOUS EXTRACT OF LEAVES OF Artemisia vulgaris ANDRÉIA LIMA DE AMORIM; ABRAÃO ALVES PINHEIRO; ROSANY LOPES MARTINS; ALEX BRUNO LOBATO RODRIGUES; ÉRICA DE MENEZES RABELO; SHEYLLA SUSAN MOREIRA DA SILVA DE ALMEIDA. Unifap, Macapá - AP - Brazil. Introduction and objective: Artemisia vulgaris (Compositae), popularly known as wild wormwood, is a perennial herbaceous of Asian origin and cosmopolitan distribution. Usually used in oral or topical forms, it has several indications in the popular use as analgesic, antispasmodic, antiparasitic and cicatrizant. The objective of this work was to investigate the phytochemical profile and to evaluate the cytotoxicity of the crude aqueous extract (CAE) of A.vulgaris leaves against Artemia salina larvae. Materials and methods: Phytochemical prospecting was performed according to the methodology proposed by the Brazilian Pharmacopoeia (2010) and Mâcedo (2005), and 14 secondary metabolites were investigated. The assay of cytotoxicity against larvae of A. salina was based on the methodology of Araújo et al. (2010) and Lôbo et al. (2010), using 7 test groups with concentrations of 1250, 1000, 500, 250, 100 and 50 μg.mL-1 of CAE and 1 control group (DMSO 5%). After 24 h, the numbers of dead individuals were counted, considering as such those totally immobile. The lethal concentration (LC50) was determined using the SPSS (Statistical Package for the Social Sciences) program. Results and conclusion: In phytochemical prospecting only the tests for alkaloids, proteins and amino acids, saponins and tannins proved to be positive, corroborating some traditional uses. In relation to the bioassay of cytotoxicity, the CAE of A.vulgaris leaves showed LC50 of 4002 μg.mL-1, being considered non-toxic according to Nguta et al. (LC50 1000 μg.mL-1). Financial support or fellowship: Tutorial Education Program (PET) - Pharmacy/UNIFAP. CFSP306 PHYTOCHEMICAL STUDY AND PRELIMINARY EVALUATION OF CYTOTOXIC ACTIVITY OF AQUEOUS RAW EXTRACT OF LEAF OF Eryngium foetidum L. (APIACEAE) ABRAÃO ALVES PINHEIRO1; ANDRÉIA LIMA DE AMORIM2; ALEX BRUNO LOBATO RODRIGUES2; ROSANY LOPES MARTINS2; ÉRICA DE MENEZES RABELO2; SHEYLLA SUSAN MOREIRA DA SILVA DE ALMEIDA2. Universidade Federal do Amapá, Santana - AP - Brazil; 2Universidade Federal do Amapá, Macapá - AP - Brazil.

Introduction and objective: Known in the north of Brazil as chicory, chicory of Pará or chicory of the Amazon, Eryngium foetidum L. (Apiaceae) is a common vegetable in tropical regions, employed in cooking or as a medicinal plant. This species is used in folk medicine for the treatment of grippe, fevers, stomach pains, birth facilitator and aphrodisiac. Thus, this work aims to carry out the phytochemical study and preliminary evaluation of toxicity, using Artemia salina, from the aqueous extract of the leaves of E. foetidum L. Materials and methods: The phytochemical analyses of the obtained extract were performed with the use of specific reagents and developers according to the methodology proposed in the Brazilian Pharmacopoeia (2010) and Macedo (2005). The toxicity test was performed at concentrations of 1250, 1000, 500, 250, 100, 50 and 10 µg.mL-1 and control (DMSO 5%). The tests were performed in triplicate. After 24 hours the dead individuals were counted. The lethal concentration causing 50% mortality in the population (LC50) was determined through the PROBIT analysis using the SPSS® software (StatisticalPackage for the Social Sciences). Results and conclusion: The preliminary phytochemical results of the aqueous extract of E. foetidum leaves presented positive results for proteins and amino acids, saponins, reducing sugars and depsides and depsidones. In relation to toxicity, the aqueous extract of E. foetidum showed LC50 of 2955µg.mL-1. According to data already described in the literature for this methodology of preliminary evaluation of toxicity, extract with CL50 > 1000 µg.mL-1 are considered to be non-toxic. Financial support or fellowship: PET-Farmácia. Universidade Federal do Amapá-UNIFAP

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CFSP307 PRE-FORMULATION AND FORMULATION OF FREEZE-DRIED SOLUTIONS USING BOVINE SERUM ALBUMIN AS A MODEL DANILO COSTA GERALDES1; LAURA DE OLIVEIRA NASCIMENTO2; GIOVANNA SEVILHA FERREIRA3. IB - Unicamp, Campinas - SP - Brazil; 2Fcf - Unicamp, Campinas - SP - Brazil; 3Fcf- Unicamp, Sorocaba - SP - Brazil.

Introduction and objective: : Heat, pH changes and eamination can denature proteins in aqueous diluents; for this reason dehydration by freeze-drying is the most used process to preserve biological drugs. In order to carry out the process without protein stability damage, the solutions of pre-freeze-drying formulations must contain suitable excipients to protect the molecule of interest that will not interfere with the process or storage of the final product. The presented project aims to develop studies of pre-formulation using design of excipients to evaluate different combination of lyoprotector excipients to the maintenance of the model protein albumin’s properties. Materials and methods: Alanine, glycine, sucrose and trehalose were combined by design of experiments and had their collapse temperature evaluated by freeze-drying microscopy. Bovine serum albumin was combined with the excipient mixtures and the freeze-drying carried out in a 96 well plate in the pilot lyophilizer Lyostar III (SPscientific). Analysis of total protein (UV/vis method), Raman Spectroscopy and appearance of the freeze-dried product (collapse, color, and volume) were performed as quality parameters. Results and conclusion: The collapse temperature of individual excipients was superior to -40 ºC (alanine -4.4ºC, glycine -12.7ºC, sucrose -30.2ºC and trehalose -24.3ºC), however, the combination between two excipients showed a collapse temperature inferior to -55.0 ºC. Visual evaluation showed that combinations containing only sucrose, only trehalose, 62.5% sucrose, 62.5% trehalose, glycine+sucrose, glycine+trehalose, sucrose + trehalose and only albumin collapsed. The Raman Spectroscopy analysis could not detect the albumin’s specters, however the excipient signals could be well seen. Unexpectedly, the majority of the mixtures presented a different total protein result after lyophilization. The development of this project will bring profound knowledge about the process stages of this kind of formulation, resulting in changes for its optimization and rational development of the formulation of this type of product. Financial support or fellowship: FAPESP; CAPES CFSP308 PRELIMINARY STABILITY EVALUATION OF DERMATOLOGIC HEALING EMULSION CONTAINING ARCTIUM LAPPA EXTRACT 10% ANDRÉIA DE HARO MORENO; SÔNIA REGINA DE OLIVEIRA BERTOLUCI; VICTÓRIA LARISSA DOS SANTOS. Fipa, Catanduva - SP - Brazil. Introduction and objective: Arctium lappa is a medicinal plant known as bardana with antiseptic, anesthetic and anti-inflammatory properties confirmed by several studies, and added in dermatological formulations such as glycolic extract or tincture for the treatment of injuries and burns. However, the formulation must be stable during the time of use, without physicochemical or microbiological changes. The objectives of this study were to develop a dermatological healing emulsion containing Arctium lappa extract 10% and evaluate the physicochemical and microbiological preliminary stability after temperature exposure for 15 days. Materials and methods: Ethanolic extract at 20% was obtained by percolation technique; dermatologic formulations were prepared by the standard technique of emulsion preparation. The extract was added at 10% and formulation samples were subjected to three different temperature conditions (0ºC, 25º and 40ºC) for 15 days. Physicochemical parameters were evaluated, such as organoleptic aspects, pH, density and coalescence. Microbiological stability was evaluated using Pour Plate pharmacopeial technique for total aerobic microbial count and specific tests for the pathogenic microorganisms. Results and conclusion: It was observed that the dermatological healing emulsion remained stable in terms of pH, density and macroscopic features, and for microbiological testing and for the presence of pathogenic microorganisms, presenting no risk of contamination with its use. Thus, the preliminary stability evaluation of pharmaceutical formulations is very important to verify the physicochemical properties and contamination levels and establish control standards to secure the quality and safety of pharmaceuticals storage. Financial support or fellowship: FIPA

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CFSP309 PREPARATION AND CHARACTERIZATION OF NATURAL RUBBER MEMBRANES WITH VARIABLE CONCENTRATIONS OF AQUEOUS EXTRACT OF POLYMERIZED PROPOLIS AT DIFFERENT TEMPERATURES BRUNA DANIELE DOS SANTOS1; ANA RAQUEL ROCHA MENEZES2; TAMIRES KRUPP1; JOSMARY RODRIGUES SILVA1; NARA CRISTINA DE SOUZA1; PAULA CRISTINA DE SOUZA SOUTO1. Universidade Federal de Mato Grosso, Barra do Garças - MT - Brazil; 2Universidade Federal de Mato Grosso, Barra do Garças - Brazil. 1

Introduction and objective: Natural rubber comes from rubber latex Hevea brasiliensis, which is important in the development of bandages due to its elasticity, flexibility, impermeability and easy adhesion to tissue. Propolis is extracted by bees and its therapeutic activities are associated with flavonoids, which are responsible for antimicrobial, antifungal and anti-inflammatory action. Thus, the objective of this study was to develop and characterize natural rubber membranes of rubber tree Hevea brasiliensis and aqueous propolis extract of Scaptotrigona polysticta with different concentrations and temperatures of polymerization for the development of a new bandage. Materials and methods: Hevea brasiliensis latex was obtained from rubber tree of RRIM-600 clones. The propolis used came from the species Scaptotrigona polysticta. After obtaining the propolis, an aqueous extract containing 10g of propolis was prepared for each 100 ml of water. The membranes were prepared with proportions of 5%, 10% and 15% aqueous propolis extract in latex. Then, the mixture was poured into petri dishes and taken to the polymerization oven at temperatures of 27°C and 60°C. Membrane characterization was performed by wettability tests and optical microscopy. Results and conclusion: In the wettability test, it was observed that the latex membranes increase hydrophilicity with the addition of propolis, however, in the higher concentration of propolis the membranes became hydrophobic. Optical microscopy showed that, regardless of temperature, there was an increase in the number of pores with the addition. The results show the possibility of using these membranes for the treatment of skin lesions since hydrophilicity is important to maintain moisture and the porosity allows the exchange of gases in the lesion, facilitating healing. Financial support or fellowship: FAPEMAT (Process nº 227369/2015)

PRODUCTION OF A NON-IONIC BASE CREAM OF ALOE VERA FOR TOPICAL USE

CFSP310

GABRIELLY KRÍSSIA DE ARAÚJO SILVA; SEBASTIÃO RICARDO ALMEIDA MENEZES; VIVIANE PATRÍCIA BATISTA DA SILVA; YURI MARINA DA SILVA CABRAL; CARLOS EDUARDO MIRANDA DE SOUZA. Centro Universitário Tabosa de Almeida - Asces-Unita, Caruaru - PE - Brazil. Introduction and objective: Popularly known as “babosa”, Aloe Vera is a medicinal herb, which has considerable application in the pharmaceutical industry, for the production of many and different products, mainly about moisturizing, healing and antiinflammatory actions. These actions can act on the cells of the dermis helping to reduce the swelling, inflammation and redness associated with blackheads and pimples. Besides, the gel extracted from the leaves of the herb works as an astringent for the skin, so when applied to the skin, the plant removes impurities from the pores, making the skin cleaner. Objective: to develop and incorporate the “babosa” gel extract in a non-ionic cream base for topical use, through an experimental study in laboratory type and to verify the organoleptic characteristics of the final product (scatterability, pH, flowability, viscosity and density). Materials and methods: The pulp found inside the leaves of the “babosa” was removed and processed in a blender until a homogeneous product was obtained. The mixture was incorporated into a non-ionic cream base, of compatible pH. The essence was added and the cream was packed in a plastic pot. Results and conclusion: A formulation of a non-ionic dermatological cream was developed incorporated into the pulp of the herb “babosa”, which presented an expressive result with moisturizing, anti-inflammatory and healing properties that have action against skin microorganisms. After manipulation, the product was analyzed and approved for organoleptic characteristics, being stable, viscous and with easy scatterability. The product can also be considered inexpensive with good results. Financial support or fellowship: ASCES-UNITA

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CFSP311 PRODUCTION OF BIOPRODUCTS WITH ANTIMICROBIAL ACTIVITY FROM THE EXTRACT OF THE LEAVES OF Platonia Insignis Mart. (BACURI) EMMELINE DE SÁ ROCHA1; ANDERSON ASSIS DA SILVA2; JOSÉ RIBAMAR DE CASTRO JUNIOR3; LEANDRA SODRÉ FERREIRA2; NATALE CRISTINE COSTA CARVALHO3; TÁSSIO RÔMULO SILVA ARAÚJO LUZ3; ODAIR DOS SANTOS MONTEIRO3; CLÁUDIA QUINTINO DA ROCHA3; PATRICIA DE MARIA SILVA FIGUEIREDO3. UFMA, São Luis - Brazil; 2Ufma, São Luis - MA - Brazil; 3Ufma, Sao Luis - MA - Brazil.

Introduction and objective: The Platonia insignis Mart. belongs to the family Clusiaceae, is popularly called Bacuri and wellknown for having a fruit of appreciated flavor. Ethnopharmacological use is related to the use of the extract of the oil of its seeds as cicatrizant and anti-inflammatory and in the production of soap. Currently, several activities have been reported for all parts of this fruit (seed, bark and pulp). This work aimed to analyze the phytochemical profile, biological and antimicrobial activity of the leaf extract of Platonia insignis Mart and develop pharmaceutical formulations. Materials and methods: Phytochemical analysis of the extract showed the presence of phenols, condensed tannins, catechins, steroids, alkaloids, flavononols and saponins. LC-MSn analyses provided the fragmentation profiles of the compounds present in the extract. In the ethyl acetate fraction, fukugentin (morelloflavone) was identified as an important marker of the clusiaceae family. The extract presented antioxidant potential in the inhibition of 88.06% of the DPPH radical. The soap and the topical solution manipulated from the 1 and 5% extract had their stability analyzed at time zero and thirty days after formulations, besides the evaluation of the microbiological quality control. The antimicrobial activity was evaluated by the microdilution technique in broth, the hydroethanol extract (70%), the hexane, ethyl acetate and the manipulated products. Results and conclusion: All the microorganisms tested presented antimicrobial activity with MIC between 0.78 and 12.5 μg/mL except the organic fractions (ethyl acetate and hexanic) that showed no activity against Acinetobacter baumannii. The toxicity evaluated by the hemolysis technique did not demonstrate hemolytic activity up to a concentration of 100 mg/mL. Toxicity to Artemia salina showed that the extract had DL50 of 42.6 μg/mL being classified as highly toxic against Artemia salina. Pharmaceutical formulations have proved potentially viable in the development of novel products for the treatment of infectious diseases. Financial support or fellowship: Laboratório de microbiologia clínica, DEFAR- UFMA

PROPOSITION OF A TRANSDERMAL DELIVERY SYSTEM FOR DONEPEZIL

CFSP312

IZABEL TOUSSAINT MENDES1; CLÁUDIA BORGES VRANCEANU1; ANDRÉ LUIS MORAIS RUELA2; FLÁVIA CHIVA CARVALHO1; GISLAINE RIBEIRO PEREIRA1. Universidade Federal de Alfenas, Alfenas - MG - Brazil; 2Universidade Federal da Bahia, Vitória da Conquista - BA - Brazil.

Introduction and objective: Cholinesterase inhibitors, such as donepezil, were the first category of drugs approved for use in Alzheimer’s disease. However, oral administration of these drugs has been associated with high incidence of gastrointestinal adverse effects including diarrhea, nausea, and vomiting. A transdermal drug delivery system can be considered as an alternative dosage form for cholinesterase inhibitors. Objectives: To study the effect of penetration enhancers - chemicals that interact with skin constituents to promote drug flux - and to prepare and evaluate donepezil-loaded nanostructured lipid carriers (NLCs) based on these penetration enhancers. Materials and methods: Skin permeation rates of various donepezil/enhancer formulations were determined using skin of pig’s ear in Franz diffusion cells. The NLCs were prepared using the hot microemulsion technique and the mean particle size, polydispersity index (PdI), zeta potential and drug entrapment efficiency of the NLC dispersions was determined. Results and conclusion: Among the penetration enhancers screened, lecitin and oleic acid were associated with the significant enhancing effect. The NLCs were successfully prepared using the microemulsion technique and show high drug entrapment efficiency value (99.86%). The mean particle size, zeta potential and PdI values were 155.4 (± 3.325) nm, -44.3(± 1.5) mV and 0.16 (± 0.032), respectively, displaying small, homogeneous size and high entrapment efficiency. The next step will be to assess the ability of NLCs to promote transdermal absorption of donepezil through in vitro skin permeation studies. Financial support or fellowship: CAPES; CNPq; FAPEMIG

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

PUNICA GRANATUM LINN BASED-EMULGEL: PREPARATION AND QUALITY ASSESSMENT

169

CFSP313

ALBERTO GOMES TAVARES JUNIOR; NATHÁLIA DOS SANTOS PIEDADE; LÍLIAN GRACE DA SILVA SOLON; FRANCISCO FABIO OLIVEIRA DE SOUSA. Federal University Of Amapá, Macapá - AP - Brazil. Introduction and objective: Several features make medicinal plants widely used, such as access, cultural knowledge and low cost. Studies have been aiming to encounter new therapeutic agents and develop new pharmaceutical formulas. Therefore, it is necessary to guarantee its pharmacological activity, safety, chemical and biological stability. The Punica granatum Linn. (Pomegranate) is commonly used to treat several pathologies and its fruit contains secondary metabolites with important therapeutic properties. This work aimed to prepare and evaluate an emulgel containing the hydroethanolic extract of Punica granatum L. Materials and methods: The ethanolic extract of P. granatum L. was obtained by means of dynamic maceration. The extract was incorporated into the emulgel base at two different levels: 0.5% and 1% (w/v). After the preformulation studies carried out, an accelerated stability study over 3 months, in which different parameters were evaluated: color, odor, appearance, pH, spreadability and viscosity. Results and conclusion: The formulations presented satisfactory characteristics, good sensorial, technical and pseudoplastic behavior, with the best preservation observed while kept under refrigeration (4ºC) and room temperature (25ºC) and the composition at 0.5% w/v of extract the most stable. The emulgel prepared has potential use for applications in which the properties of Punica granatum are indicated. Financial support or fellowship: Institutional Scientific Initiation Scholarship Program PROBIC/UNIFAP (Nº 013/2015) and Institutional Program of Initiation Scholarships in Technological Development and Innovation PIBITI/CNPq (Nº 013/2016). CFSP314 QUALITY CONTROL OF BACHARIS TRIMERA (LESS) DC. COMMERCIALIZED IN SÃO PAULO, SP, BRAZIL. MARCIA EUGENIA DEL LLANO ARCHONDO; CAROLINE BIANCHI; ROBSON MIRANDA DA GAMA; REGINA SIQUEIRA HADDAD CARVALHO. Universidade Santo Amaro, São Paulo - SP - Brazil. Introduction and objective: Baccharis trimera (Less) DC (Asteraceae), is a medicinal plant used for hepatic disorders, stomach pain and bad digestion. Literature also describes antioxidant, anti-inflammatory and hypoglycemic properties. The objective of this work was to evaluate physicochemical and microbiological quality of Baccharis trimera commercialized in São Paulo, SP; Brazil. Materials and methods: Six Baccharis trimera samples sold as dried leaves were acquired in São Paulo. Sample packing and labeling were analyzed following current legislation for medicinal herbs. For microbiological quality, total aerobic microbial count assay, total yeast and mold count assay, and pathogens presence and identification were performed. Physicochemical quality was assessed through organoleptic properties, presence of non-identified material, humidity, total ashes, dry matter, phytochemical screening, thin layer chromatography profile and total flavonoids content expressed as routine and quantified by spectrophotometry. Results and conclusion: None of the samples complied with legal requirements for dried herb packing and labeling. Other quality non-conformities were: 66.7% high humidity samples and 16.7% microbiological contamination, absence of pathogens. Phytochemical screening was positive for tannins, flavonoids and saponins. Chromatographic profile was similar for all the samples. Total flavonoids expressed as routine between 0.167mg/g and 1.210mg/g. Results achieved indicate that even though there was no identified herb adulteration, microbial contamination, high humidity and total flavonoids content wide variation indicates possible processing and storage problems. Good practices and quality procedures are necessary in order to assure safety and efficacy of medicinal herbs. Financial support or fellowship: Universidade Santo Amaro. LESIFAR laboratory.

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CFSP315 QUALITY EVALUATION OF AZITROMYCIN POWDER FOR ORAL SUSPENSION DISTRIBUTED BY UNIQUE HEALTH SYSTEM FERNANDA FERNANDES FARIAS; VALERIA ADRIANA PEREIRA MARTINS; ELIANA DELLA COLETTA YUDICE; STELA FERNANDES HIDALGO; LUZ MARINA TRUJILLO. Instituto Adolfo Lutz, São Paulo - SP - Brazil. Introduction and objective: Azithromycin is a broad-spectrum antimicrobial derivative from erythromycin, indicated for the treatment of respiratory tract infections, skin and soft tissue infections, and sexually transmitted diseases. It is marketed in pharmaceutical forms of powder for oral suspension, coated tablet, capsules, lyophilized powder for solution for injection and oral suspension. The objective of this study was to evaluate the quality and to critically analyze the results of four different batches of azithromycin 200mg/5mL, powder for oral suspension, distributed by the unique health system and received by Adolfo Lutz Institute from 2016 to 2017 that presented as technical complaint lumps in the powder and oral suspension. Materials and methods: The following tests were prioritized for this investigation: aspect by visual inspection, comparing the results of analysis with the information described in the package insert; weight determination and pH according to specification of the Brazilian Pharmacopoeia 5ed; reproduction of use that represents how the medicine will be prepared and used by the patient or health professional for administration, according to the information of package insert. Results and conclusion: The results were satisfactory for weight determination and pH tests and unsatisfactory for appearance and reproduction of use. The presence of lumps in 18 of 35 analyzed bottles was confirmed and it was not possible to extract the three doses referring to complete treatment of the drug, compromising efficacy. Although the legislation regulating manufacture and distribution of medicines in Brazil has evolved and is internationally recognized due to the high stringency of requirements, failures to comply with quality requirements are still identified. These results demonstrate the importance of sanitary surveillance activities for monitoring the quality of medicines distributed in the public health system ensuring that the population receives reliable products.

QUALITY EVALUATION OF NEW AND USED NAIL POLISH FROM BEAUTY SALONS.

CFSP316

MARCOS AUGUSTO DE ASSIS BRASILEIRO1; ANNA BEATRIZ GOMES DA SILVA1; JULIANO MORAIS FERREIRA DA SILVA2; MÔNICA VALERO DA SILVA1. Unb, Brasília - DF - Brazil; 2Cassi, Brasília - DF - Brazil.

Introduction and objective: Nail polish is a type of cosmetic frequently used in beauty salons, as a means of personal beautification. In these salons, the same nail polish may be used several times by multiple clients and as such, may represent a risk for onychomycosis dissemination. These, in turn, may become a challenging for dermatologic therapy with both topic and oral antifungal medicine. The aim of this work was to evaluate quality and safety of both used nail polish from beauty salons, and new nail polish, commercially acquired. Materials and methods: A total of 72 samples of both new and used nail polish were analyzed. New nail polish of three different colors (black, light pink and transparent) from three different brands (Risqué, Impala and Colorama) were analyzed. For the microbial challenge, Epidermophyton floccosum, Microsporum canis and Candida albicans, were repeatedly inoculated at 108 CFU/mL. Microbial count per area was performed on colonies grown on sabouraud dextrose agar (SDA), from both used nail polish (donated by beauty salons) samples and newly acquired nail polish samples, without any previous inoculation. For the challenge test, microbial strains were inoculated at different times (07, 14, 21 and 28 days), in new nail polish flasks, and were plated on SDA dishes, in duplicate. The incubation was carried on for 14 days, at 25±2°C. Results and conclusion: There was contamination in 27.8% (5 samples) of used nail polish samples donated from beauty salons. Of the new nail polish which had not been previously contaminated, 11.11% (3 samples) showed contamination characteristic of filamentous fungi. In the samples which were previously inoculated with different strains of fungi, growth was observed in 100% inoculated with C. albicans, 66.7% with E. floccosum and 88.9% with M. canis. There is no safety in the collective usage of nail polish in beauty salons. The solvents found in the polish composition were not capable of inhibiting fungal proliferation. We propose that nail polish composition should be re-evaluated by the cosmetics industry, in order to reduce contamination and dissemination risks.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

RESEARCH OF STAPHYLOCOCCUS AUREUS IN TYPICAL JAPANESE FOODS

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CFSP317

JOSÉ CARLOS BERNARDO DA SILVA FILHO 1; ADRIANA EUN HE KOO YUN 2; ALESSANDRA ROSELI GONÇALVES DE SANTANA2; LÍVIA ALBUQUERQUE SILVA2; AGENOR TAVARES JÁCOME JÚNIOR2. Asces-Unita, Jurema - PE - Brazil; 2Asces-Unita, Caruaru - PE - Brazil.

Introduction and objective: The typical food(s) of Japanese cuisine are usually prepared manually, based on raw fish, Japanese rice and kelp. They are considered highly perishable products. The presence of Staphylococcus aureus, found on the mucosa and surface of human skin, plays a role both as a pathogen and as a hygienic-sanitary indicator related to inadequate handling. This bacterium finds in the fish a favorable environment for its multiplication. Some strains of Staphylococcus aureus produce a thermostable enterotoxin, responsible in man for food poisoning. The objective of this work is to investigate the presence of the Staphylococcus aureus bacteria in sushi and sashimi marketed in restaurants that specialize in typical Japanese food located in the Agreste Region of Pernambuco. Materials and methods: The study was of the laboratory type (experimental), the choice of the sample was for convenience. The samples were selected and collected according to the sampling plan described in the Codex Alimentarius, collecting pieces of sushi or sashimi that had a good organoleptic state, with characteristic color, taste and odor. The quality parameters of the samples were analyzed according to ANVISA Resolution-RDC No. 12 of January 2, 2001. The Staphylococcus aureus bacterium research was performed by counting colonies in Baird-Parker agar medium. Results and conclusion: The presence of Staphylococcus aureus was obtained in 75% of the self-service samples, 44.5% of the samples a la carte and 50% of the Delivery samples. Professionals responsible for the handling and distribution of raw fish products are advised to adopt more stringent hygienic-sanitary measures through the adoption and application of Good Manufacturing Practices (GMP), in order to control the possible potential risks to consumer health. It is also recommended that the health authorities pay more attention to establishments selling raw fish dishes. Financial support or fellowship: Asces-Unita

ROOT VEGETABLE YIELD OF SOLANUM PANICULATUM L. (JURUBEBA)

CFSP318

DAYANA GRAZIELA COSTA 1; JOICE LUIZA PEREIRA DA SILVA 2; IZABELLA CINTHIA TÔRRES DE VASCONCELOS2; BRUNNA LARISSA DE SOUZA MELO FERREIRA2; VANESSA CAMYLLA BERNARDO DE OLIVEIRA2; ANALÚCIA GUEDES SILVEIRA CABRAL2. Asces/Unita, Caruaru - Brazil; 2Asces/Unita, Caruaru - PE - Brazil.

Introduction and objective: Solanum paniculatum L. belongs to the Solanacea family and is known as “Jurubeba”. It is widely used in Brazil and besides that is a distinct plant due to its important medicinal applications, large distribution and, specially, for being recognized as a phytotherapeutic agent by the Brazilian Pharmacopoeia. Taking into account the aforementioned characteristics, it was aimed to conduct a study of the yield of the dry crude root extract of jurubeba. Materials and methods: The root of this plant was collected and weighed to a total of 1.018g. The material was triturated and then macerated for 7 days. The extrusion and evaporation of the extractive solution were carried out until loss of 95% of its humidity. Afterwards, the solution was placed inside a BOD incubator in order to obtain the dry crude extract. The yield of the extract was calculated according to the formula: Re = (weight of dry crude extract/weight of fresh leaves) x 100. Results and conclusion: The result obtained for the yield of the root (%) was = 1.47%. This result shows a low yield related to the plant part studied. Due to this, it is necessary to carry out further scientific studies with a greater amount of plant material in order to obtain a better yield. Financial support or fellowship: ASCES/UNITA

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CFSP319 SELECTION OF ANTINEOPLASTIC DRUGS FOR THE DEVELOPMENT OF VALIDATION STUDIES OF STORAGE TIME AFTER PREPARATION. CARLOS EDUARDO PEIXOTO DA CUNHA; BRUNA DA SILVA CANEDO DINIZ. Universidade Federal de Goiás-Ufg, Goiânia - GO - Brazil. Introduction and objective: The World Health Organization (WHO) points to cancer as responsible for the death of 16 million people a year around the planet, being a disease of varied causes. Among the safest and most effective treatments available, chemotherapy is widely used in hospital protocols. They are high-cost drugs and should be consumed in their entirety, however, often their preparation must meet the medical prescription, which causes the waste of some of these drugs. The objective of this research was to select possible antineoplastic drugs, which can be validated regarding the storage time, through the study of extended stability. Materials and methods: This study was exploratory field research, developed between February and April, in a pharmacy located in an oncological hospital unit of the city of Anápolis, in the state of Goiás, for outpatient care, prevention, diagnosis and treatment. Treatment of cancer. The drugs were selected by the number of times they were dispensed in the evaluated period, those judicialized were disregarded. Results and conclusion: During the period evaluated, 25 drugs were formulated with different active principles. Of these, 3 were prescribed for more than one patient: Cisplatin (4), Doxorubicin (7), and Paclitaxel (9). Although the practice of extended stability has not been widely disseminated, it was possible to note the importance of ensuring the safety and efficacy of an antineoplastic drug, considering the needs of patients who need accessible and well-controlled cancer therapies. As for the sake of pharmacoeconomics. Financial support or fellowship: Federal University of Goiás, Goiânia, GO, BRAZIL CFSP320 S E R O T O N I N E R G I C P A T H W A Y A C T I VA T I O N M E D I A T E S T H E A C T I V I T Y O F 4-METHYLBENZENECARBOTHIOAMIDE IN EXPERIMENTAL MODEL OF INFLAMMATORY PAIN IVO SOUZA FERRAZ DE MELO; SARAH OLIVIA ALVES MENDES DA COSTA; JÉSSICA APARECIDA VAZ; ALYSSON VINÍCIUS BRAGA; FELIPE FERNANDES RODRIGUES; MARCELA ÍSIS MORAIS; RENATA BARBOSA DE OLIVEIRA; RENES DE RESENDE MACHADO; MÁRCIO DE MATOS COELHO. Universidade Federal de Minas Gerais, Belo Horizonte - MG - Brazil. Introduction and objective: Hydrogen sulphide (H2S) plays important roles in pathophysiological processes, such as inflammation and pain. Since thiobenzamides act as H2S donors, we evaluated the effects induced by 4-methylbenzenecarbothioamide (4MBC) in a model of inflammatory pain in Swiss mice and also whether serotonin or opioid receptors or KATP channel activation mediates its activity. Materials and methods: 4-MBC (50, 100 or 150 mg/kg) was administered intraperitoneal (i.p.) 30 min before carrageenan (Cg; 300 μg/20 μl, intraplantar). The paw withdrawal threshold (expressed in grams) of each animal was measured at 2, 4 and 6 h after Cg injection. To investigate mechanisms mediating the activity of 4-MBC, naltrexone (5 or 10 mg/kg), glibenclamide (20 or 40 mg/kg) or cyproheptadine (5 or 10 mg/kg) were administered i.p. 30 min before 4-MBC (150 mg/kg). This study was approved by the Ethics Committee on the Use of Animals of UFMG (Certificate 22/2016) Results and conclusion: 4-MBC (150 mg/kg) increased the nociceptive threshold evaluated at 2, 4 and 6 h after Cg injection. The activity of 4-MBC was not altered by previous administration of naltrexone or glibenclamide. However, cyproheptadine markedly reduced the activity of 4-MBC in the experimental model. In conclusion, we demonstrated that 4-MBC exhibits antiallodynic activity and that this activity may be mediated by activation of serotonergic receptors. These results reinforce the interest in the investigation of H2S donors, such as thiobenzamides, as candidates for analgesic drugs. Financial support or fellowship: FAPEMIG, CNPq, PRPq/UFMG, CAPES

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CFSP321 STUDY OF THE YIELD OF THE DRY CRUDE LEAVES AND BRANCHES EXTRACTS OF ANNONA SQUAMOSA L. (PINHA) DAYANA GRAZIELA COSTA; MARIA EDUARDA SILVA AMORIM; RAISSA FERREIRA SOARES; SARA BATISTA VASCONCELOS; VANESSA CAMYLLA BERNARDO DE OLIVEIRA; BRUNNA LARISSA DE SOUZA MELO FERREIRA; RISONILDO PEREIRA CORDEIRO; JÚLIA SAMARA FERREIRA DA SILVA. Asces/Unita, Caruaru - PE - Brazil. Introduction and objective: Annona squamosa L., popularly known in Brazil as Pinha, is native to Central America and is traditionally used for the treatment of chronic diseases. Taking into account the aforementioned characteristics, it was aimed to conduct a study of the yield of the dry crude leaves and branches plant extracts of Annona squamosa L. Materials and methods: Leaves and branches of the aforementioned vegetable were collected and weighted, totalizing 354 g and 773 g, respectively. Both materials were triturated and then macerated for 7 days. The extrusion and evaporation of the extractive solutions were carried out until the loss of 95% of humidity. In sequence, the solutions were placed inside a BOD incubator in order to obtain the DCEs. An aliquot of 0.5 g of each solution was placed inside a laboratory stove at 100º C for 1 h. After cooling, the materials were weighed every 30 minutes until 3 consecutive equal results were obtained. The calculation was performed taking into account the weights measured for each step of the process and the results were expressed as percentages. Results and conclusion: As a result, for the yield of the branches (%): 3.3% was obtained and for the leaves (%): 4.7%. These results demonstrate low yields, for both branches and leaves, which may be related to the water content of the vegetable. Due to this, it is necessary to carry out further scientific studies with a greater amount of plant material in order to obtain a better yield. Financial support or fellowship: ASCES/UNITA CFSP322 SUSTAINABILITY CHALLENGES AND THE QUALITY OF LIFE OF RIPARIANS IN THE ITAITUBA/PARÁ MICROREGION FERNANDO LUCAS; AMANDA GONÇALVES BENTO; DIONE NATUREZA DE MORAES. Faculdade de Itaituba, Itaituba - PA - Brazil. Introduction and objective: Sustainable development is a responsibility of all, since every human being enjoys the resources that are available by nature, and in the same way that the individual enjoys the goods he attacks in all the ways that suit him, pollutes, deforests, destroys, among other acts. From this perspective, each one has the obligation to preserve to be able to survive without needing something or someone that forces it to do so. To present theoretical approaches on the proposals of interest of the organizations, and to mention the results coming from projects that have influenced the quality of life of riparians, in addition, to describe the characteristics of the population in this process of social integration. Materials and methods: It is a functionalist orientation of research that centralizes the relationship between cause and environmental effects, being a dimension little explored in the field of current organizational research, which referred to three study procedures: the syncretic view - a search for sources that are close theoretically on the subject of interest; Analytical vision - critical or reflective positioning on the texts that are selected by the researcher and the synthetic vision - interpretation and synthesis of data, to have a final and more exact understanding. The municipality of Itaituba of the State of Pará has a strong culture regarding agricultural activity and its index as a rural field is high, so it is important to relate the citizen’s experience with the possibilities of growth within the framework of sustainable development, because the economic production Has favored the development of the microregion and are aspects that need to be unveiled and divulged so that other perspectives can unveil new inquiries and thus contribute to the future Brazilian social, cultural, economic and political scenario, integrating different and diverse realities to the globalized world context . It was possible to verify that sustainable projects are very present in the policy of multinational companies that recently settled in the region. However, it has reflected on improving the company’s image vis-à-vis society, increasing the company’s profitability and competitiveness in the market in which it operates, and on the other hand, citizen’s quality of life has been stuck with high taxes with little return on resources which should be more viable for the population. Results and conclusion: One of the challenges of environmental sustainability is the awareness that this is a process to be followed and not something definitive to be achieved. While it can not by itself reverse the current scenario of unsustainability, we have much to contribute to making societies sustainable. In such a way, it is necessary to train people capable of reflecting on the way these societies are formed, and from this reflection to seek democratic solutions, since society has to be attentive to the most important focus of all its spheres: the quality of Life of the human being.

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CFSP323 SYNTHESIS OF A HYBRID WITH PRODRUG FEATURES OF PARACETAMOL ASSOCIATED WITH A NITRIC OXIDE DONOR MOLECULE. LEONARDO RIBEIRO BERNARDO; JOÃO GABRIEL GOUVEA DA SILVA; ANTÔNIO JOSÉ CALIXTO DE SOUZA. Universidade Católica de Santos, Santos - SP - Brazil. Introduction and objective: Paracetamol is an analgesic of consecrated use, but as every drug has its side effects, among them we have its hepatotoxicity and one of the ways to circumvent its effect is the use of cytoprotective compounds, such as nitric oxide. Objective: To synthesize, purify and identify a hybrid of the paracetamol molecule associated with benzofuroxane, Materials and methods: To obtain the hybrid: Benzofuroxan-5-carboxylic acid was obtained from 4-amino-3-nitrobenzoic acid and then the corresponding acid chloride was generated, finally this molecule was placed to react with paracetamol. Paracetamol phenoxide was also obtained and reacted with the acid chloride in parallel. For analytical purposes, chromatographic methods (thin layer, column and gas) and mass spectrometry were used. Results and conclusion: As a final result in both synthetic routes proposed for a hybrid obtainment, solids as main products were obtained after purification by column chromatography, the product of the route with paracetamol was recrystallized and analyzed by coupled gas chromatography with mass spectrometry, indicating a result of the suggested hybrid of paracetamol with an insertion of two molecules of the nitric oxide donor structure, it should be emphasized that both, as effective routes, deserve further study as a way of improving the yield in obtaining the Hybrid proposed. Financial support or fellowship: UNISANTOS CFSP324 SYNTHESIS OF A PRODRUG BETWEEN MOXIFLOXACIN AND A NITRIC OXIDE DONOR WITH POTENTIAL TUBERCULOSTATIC ACTIVITY RICHARD FERNANDO DE ANDRADE ÁLVARES; THOMAS WAENY CORRÊA; MILTON DOS SANTOS JANUÁRIO DE ALMEIDA; ANTÔNIO JOSÉ CALIXTO DE SOUZA. Catholic University Of Santos, Santos - SP - Brazil. Introduction and objective: Tuberculosis is one of the most devastating diseases in history and was shown to be an even bigger health issue when HIV emerged. The etiological agent, Mycobacterium tuberculosis, has been showing significant resistance, due to multiple factors, to the standard therapy. Objective: A pro-drug, which emphasizes the synergy between both molecules that compose it, was proposed and synthesized. One of the components induces the bacteria to its latent form and lead to death afterwards, while the other exerts its activity while the bacteria is in the anaerobic (latent) form. It is important to state that neither of these substances interacts with antiretroviral therapy. Nevertheless, the final compound may act in both aerobic and anaerobic phases, potentially reducing the treatment time without interfering with HIV treatment. Materials and methods: The strategy to synthesize the desired compound was executed starting from two commercially available molecules, 4-chloro-3-nitrobenzaldeid, precursor of the benzofuroxan (BZF) derivative and moxifloxacin (MXF). Both molecules were esterified by two different routes, for comparison purposes. Results and conclusion: Both synthetic routes presented good potential for obtaining the desired product. However, the second showed better yield. The first route consists of a Nucleophilic Acylic Substitution (NAS) between acyl chloride (MFX) and primary alcohol (heterocyclic). The inefficiency of this reaction, which led to low yield afterwards, was related to the non-specificity reaction site. On the other hand, the second route, which consists of a Nucleophilic Substitution (Ns) between a carboxylate of (MFX) and the halogenated compound (BZF), demonstrated a much more controlled nucleophilic activity, therefore leading to a better yield.

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CFSP325 SYNTHESIS OF PHARMACOLOGICALLY ACTIVE COMPOUNDS VIA SUZUKI REACTION IN WATER TAYNNÁ DA CRUZ PEREIRA1; EVALDO BARROS MONTEIRO JÚNIOR1; SERGIO AKINOBU YOSHIOKA2; IRLON MACIEL FERREIRA1. Universidade Federal do Amapá, Macapá - AP - Brazil; 2Universidade de São Paulo, São Carlos - SP - Brazil.

Introduction and objective: The Suzuki reaction, which makes use of Palladium (Pd) as catalyst and phenylboronic acid, has been applied in the synthesis of natural products and pharmaceutical intermediates. In this sense, the objective of this work is the use of a Pd catalyst in a heterogeneous form so that it can be reused in the Suzuki reaction using water as a solvent to reduce the aggression to the environment, thus obtaining pharmacologically active compounds. Materials and methods: Fibroin was extracted from the cocoon of the silkworm (Bombyx mori) to be used as support for the Pd catalyst. The dry fiber was added to a Pd/C solution for Pd impregnation in the fiber. The fiber was characterized by Scanning Electron Microscope (SEM). The Pd/C fiber was used in the Suzuki reaction, adding 4-bromobenzaldehyde, phenylboronic acid, K2CO3 and water: glycerol 1: 1 as solvent. The reaction was stirred for 3 h at room temperature. Then the reaction was analyzed by thin layer chromatography (TLC) and by melting point, comparing with the literature. The excess solvent was evaporated to determine the yield of the reaction. Results and conclusion: By the SEM analysis, the presence of adhered Pd in the silk fibroin can be evidenced. The fiber incorporated with palladium was used in the Suzuki reaction. The yield of the formed product was approximately 50%. This result demonstrates that silk fibroin can become an important biomaterial capable of encapsulating materials such as Pd, transforming it into a heterogeneous catalyst capable of being used in several cycles and maintaining the reactive capacity of the catalyst. Financial support or fellowship: Universidade Federal do Amapá. CFSP326 THE ANTIMICROBIAL ACTION OF ESSENTIAL OILS AGAINST THE STRAINS OF PROPIONIBACTERIUM ACNES AND STAPHYLOCOCCUS AUREUS EMMILY DANTAS ROCHA; SARA DE SOUZA PRATES; BRUNA SILVA GONÇALVES; GABRIELE ANTONIOLLI CHARELLI; CAROLINE TIEMI OYA; RENATA MIYUKI TAKARA; ANDERSON FERNANDES DE CARVALHO. University Anhembi Morumbi, São Paulo - SP - Brazil. Introduction and objective: Propionibacterium acnes is a Gram-positive bacterium that has inflammatory and immunomodulatory properties and its pathogenicity is associated with Acne Vulgaris. The growth of this bacteria could result in lesions and inflammation of the hair follicles that cause comedones and pustules. As an opportunistic agent, Staphylococcus aureus is responsible for secondary infections and the objective was to investigate the efficacy of the antimicrobial action of Cinnamon, Clove and Melaleuca Essential Oils against P. acnes and S. aureus strains. Materials and methods: P.acnes strains ATCC 6919 and S.aureus ATCC 25923; Essential Oils of Clove, Cinnamon and Melaleuca from WNF supplier; Streptomycin + Penicillin used for comparative control. The tests were performed according to NCCLS/CLSI M2-A8 and M7-A6 methods, respectively, to investigate the antimicrobial action of Essential Oils: Muller Hilton Agar diffusion from Acumedia supplier and determination of Minimal Inhibitory Concentration (MIC) in a 96 well plate by microdilution method. Results and conclusion: Through the diffusion in Agar we observed that both Essential Oils formed inhibition halo, however, Cinnamon Oil presented a minimum halo of 30 mm against P. acnes, superior when compared to the Inhibition halo Of Streptomycin + Penicillin that was 22mm in the same strain. Concerning to MIC test, bacterial strains tested of P. acnes and S. aureus obtained the following profile of antimicrobial activity: Cinnamon Oil and Blend (Cinnamon + Clove oil) had equivalent action, being superior to Melaleuca which was higher than Clove Oil. Therefore, it is possible to suggest that Cinnamon Essential Oil presents an important antimicrobial activity against different bacterial species, being a product used that could be in different pharmaceutical formulations.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

THE IMPORTANCE OF QUALITY CONTROL OF RADIOPHARMACEUTICALS

176

CFSP327

ANA CAROLINA FERREIRA 1; SUZANE GARCIA FERREIRA 2; THALITA GONÇALVES DO NASCIMENTO CAMILO3; MATHEUS MENEZES VIANNA1; ISIS CAROLINE ROSA DOS SANTOS4; THAIS RIBEIRO PINTO BRAVO2. Centro Universitario Anhanguera Educacional Niteroi, Niteroi - RJ - Brazil; 2Universidade Federal Fluminense, Niteroi - RJ BraBrazilsil; 3Universidade Federal Fluminense, Rio de Janeiro - RJ - Brazil; 4Instituto Brasileiro de Medicina e Reabilitação, Rio de Janeiro - RJ - Brazil. 1

Introduction and objective: Radiopharmaceuticals are pharmaceutical preparations which have at least one radioactive nuclide in their composition and are used in Nuclear Medicine Services (NMS) for diagnosis and/or therapy. They can be prepared in industrial, centralized and hospital radiopharmaceuticals duly regulated by specific organs such as CNEN and ANVISA. According to RDC38/2008, the NMSs are responsible for the quality control of generators and radiopharmaceuticals as described by the manufacturers, either by regulated package inserts or pharmacopeia. The general objective of the work is to establish the importance of the quality control of radiopharmaceuticals. Materials and methods: The work evaluated the marking of 17 samples by radiochemical purity and pH of 99mTc-FITATO performed by the Nuclear Medicine Sector of Hospital Antônio Pedro in Niterói in the years of 2015 and 2016. Results and conclusion: The results indicate the radiochemical purity (mean = 99%) and the pH values (mean = 5.35) were within the recommended range. Performing quality controls of radiopharmaceuticals adequately minimizes or even ceases the possibility of undesirable images observed in nuclear medicine clinical procedures. In addition, it helps to establish radiopharmaceutical procedures, which are very important for high quality procedures in nuclear medicine. CFSP328 OUTSOURCING IN THE BIOMEDICINE MANUFACTURING SECTOR AND THE USE OF CMOs (CONTRACT MANUFACTURING ORGANIZATIONS) FELIPE RODRIGUES DA SILVA. Fiocruz/UFRJ, Rio de Janeiro - RJ - Brazil. Introduction and objective: The decision between doing an activity internally or outsourcing it is considered strategic to an organization. The purpose of this work was to study the current situation of the biopharmaceutical industry, seeking to understand how, why and where the organizations of the area are choosing to outsource their production activities. Considering a recent tendency to the movement, several companies (called CMOs) are specializing in providing production services. Materials and methods: The research used as main sources of information the literary references and the data obtained in the field with specialists in the area. The field research was carried out through the distribution of questionnaires in preliminary phases (Phases I and II) and semi-structured interviews directed to specialists working in the area in the last and main stage (Phase III). Results and conclusion: The occurrence of the movement was confirmed in biopharmaceutical companies, mainly influenced by issues of cost, focus on core activities, operational flexibility and long-term strategies. The steps of scale up/batches production for phase III clinical trials were identified as the most frequently outsourced, followed closely by the final processing and, finally, the complex activities for API (Active Pharmaceutical Ingredient) manufacturing. The study demonstrated the need for robust systematic processes for selecting CMO companies and emphasized the importance of transparency in outsourcing contracts. It also evaluates the interference of circumstances related to the complexity of biological processes, company size, geographic location and the influence of intellectual property issues. It was possible to conclude that the decision to outsource productive activities in biopharmaceutical companies should be carefully evaluated and depends on the analysis of all the circumstances addressed in this work. Financial support or fellowship: Fundação Oswaldo Cruz (Fiocruz)

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

THE PRODUCTION OF ALOE VERA GEL FOR HAIR USE

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VIVIANE PATRÍCIA BATISTA DA SILVA; GABRIELLY KRÍSSIA DE ARAÚJO SILVA; SEBASTIÃO RICARDO ALMEIDA MENEZES; YURI MARINA DA SILVA CABRAL; CARLOS EDUARDO MIRANDA DE SOUZA. Centro Universitário Tabosa de Almeida - Asces-Unita, Caruaru - PE - Brazil. Introduction and objective: Aloe Vera is a medicinal herb, popularly known by the name of “babosa” in Brazil, it has many attributes for cosmetic and medicinal purposes and uses. It has a wide importance in the pharmaceutical industry because of its vitamins and polysaccharides. The active substances concentrated in the gel are found in the middle of the leaf and have compounds capable of providing the hair with good hydration based on humectant activities. Objective: To perform an experimental laboratory study to formulate a cosmetic gel from “babosa” for hair application and analyze its action on the scalp, and to observe and define the organoleptic characteristics of the final product (scatterability, pH, flowability, viscosity and density). Materials and methods: The mucilage contained within the aloe leaves was removed and processed in a blender until a homogeneous product was achieved. The pH of the solution was checked for compatibility with the gel base. The mixture was incorporated into a base comprising carbopol gel supports and triethanolamine. Dye and essence were added and then the gel was packed in a plastic pot. Results and conclusion: After the accomplishment of the tests, the gel had pH, scatterability and viscosity satisfactory for the defined purpose. The gel was stable and very effective with a huge moisturizing power, which guarantees several benefits such as strengthening hair strands, providing shine and softness. Besides it becomes even more feasible because it is a low cost product and can be produced with conventional equipment and techniques. Financial support or fellowship: ASCES-UNITA

THE ROLE OF LIPID PEROXIDATION PRODUCTS IN RECTAL CANCER PROGRESSION

CFSP330

MÁRCIA CRISTINA FERNANDES MESSIAS1; CÉLIO FERNANDO FIGUEIREDO ANGOLINI2; ALINE GONÇALVES MORA3; TIAGO TOMAZINI GONÇALVES4; GIAN LUCAS MELO ARRUDA4; PATRÍCIA DE OLIVEIRA CARVALHO4. Universidade São Francisco, Bragança Paulista - Brazil; 2Universidade de Campinas, Campinas - SP - Brazil; 3Universidade Sao Francisco, Bragança Paulista - SP - Brazil; 4Universidade Sao Francisco, Braganca Paulista - SP - Brazil. 1

Introduction and objective: Oxidative stress arises from an imbalance between the generation of oxidizing compounds and the performance of the antioxidant defense systems, resulting in the attack on cellular components, especially lipids. Free radicals formed in addition to inducing the lipid peroxidation process also play an important role in pathological processes, including rectal cancer. The objective of this study was to evaluate the biomarkers of oxidative stress, malondialdehyde (MDA) and F2isoprostane in the plasma of patients with rectal cancer, in order to identify changes in the lipid metabolism that may be involved in the carcinogenesis progression. Materials and methods: Blood samples from 23 patients with rectal cancer stratified according to the cancer stage and 18 healthy volunteers were used. The products resulting from the lipoperoxidation process were evaluated by determination of the Thiobarbituric Acid Reactive Substances (TBARS colorimetric) and the dosage of F2-isoprostane using an enzyme immunoassay kit. Results and conclusion: The results showed that plasma levels of MDA in patients with stage 3 and 4 rectum cancer were higher than the values observed in the healthy group and stage 0 Financial support or fellowship: CAPES; FAPESP

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

THE USE OF CARBOHYDRATE BLENDS AS A BINDER IN DIRECT COMPRESSION

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EMANUEL PATRÍCIO CASTELO BRANCO FERREIRA; CARLA LARISSA DE CASTRO VIEIRA; FRANCISCO WENDELL XAVIER BARBOSA; FRANCISCO ROGÊNIO DA SILVA MENDES; LARA THAMYRES AZEVEDO DAMASCENO; LÍVIA SOUSA UCHÔA; NÍCOLAS FIUZA RIBEIRO; THAIS ALVES LIRA; ANGELO RONCALLI ALVES E SILVA. Universidade de Fortaleza, Fortaleza - CE - Brazil. Introduction and objective: Polymers form one of the available material classes. They are used as an alternative to pharmaceutical excipients in the formulation of both conventional and modified dispensation cosmetics and drugs. Our objective is to analyze the application of carbohydrate blends as an excipient of the binder type in direct compression. Materials and methods: Three carbohydrate blends were used, they were separated into B1 (Xyloglucan of Hymenaea courbaril), B2 (Xyloglucan of Mucuna slonei) and B3 (Galactomannan of Adenanthera pavonina). At first, the blends granulometry was done to insure uniformity. After that, the tests to evaluate fluidity: angle of repose, Carr index and Hausner factor. Afterwards, a standard formulation of starch at 10% and microcrystalline cellulose s.q. was developed for 400mg tablets. For analysis, the blends were added to microcrystalline cellulose, replacing the starch. After the compression the tests of average weight, hardness and disintegration were performed. Results and conclusion: The fluidity tests showed that the samples presented an excellent flow property. In the quality control test it was observed that the B1 blend presented a greater variation of weight, 3.9%, B2 of 4.9% and B3 of 4%. As for the hardness, the standard presented the result of 5 KgF, B1 of 3.5 KgF, B2 of 4.8 KgF and B3 of 6.2 KgF. The disintegration presented a maximum time of 60 seconds ± 20. Thus, we conclude that the samples are within the professed conformity of the Brazilian pharmacopeia, being a good alternative as a binder type excipient. CFSP332 THE USE OF RICE STARCH AS A PHARMACEUTICAL EXCIPIENT IN TABLET FORMULATION AND DIRECT COMPRESSION ESTER ELAINE GONSALVES DE AGUIAR; KARINE GARGIONI PEREIRA CORREA DE MELLO; GIOVANNA BONFANTE BORINI. Universidade Nove de Julho, São Paulo - SP - Brazil. Introduction and objective: Starch is a natural substance found in vegetables in their different parts. Its physical and morphological characteristics depend on the plant from which it is extracted. This study evaluated rice starch extraction from rice flour, its characterization and its use as pharmaceutical excipient in tablets prepared by direct compression. Materials and methods: The rice starch extraction was performed according to Zavareze, 2009. The physical-chemical characterization of the rice starch obtained in the extraction was performed according to the monograph described in Farm. Bras., 2010 (identification A and B tests, pH, oxidizing substances, SO2, desiccation loss, sulfated ash). Tablets were obtained by direct compression with six different compositions with corn starch and rice and mixtures of Aerosil ®, Mg stearate, PVP K ®. The performance tests of the powder (powder flowability) and the tablets (disintegration and hardness) were performed according to Farm. Bras., 2010. Results and conclusion: The results obtained in the identification tests were according to the Farm. Bras., 2010. The yield of the starch extraction from rice flour was in agreement with the literature results. Rice starch powder and rice flour presented a better powder flowability than corn starch. Powder flowability of tablet powder mixtures containing rice starch or corn starch were also better than isolated powder samples. But after compression, all tablets did not present sufficient disintegration and hardness, indicating that rice starch is equivalent to corn starch and both were unsuitable to be used as direct compression excipient. Financial support or fellowship: Universidade Nove de Julho

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CFSP333 THERMAL CHARACTERIZATION OF A DRUG DELIVERY SYSTEM BASED ON BNZ AND ZIF-8 AIMING ALTERNATIVE TREATMENT OF CHAGAS’ DISEASE ALESSANDRA CRISTINA SILVA 1; ALINNE ÉLIDA GONÇALVES ALVES 2; LESLIE RAPHAEL DE MOURA FERRAZ2; ALINE SILVA FERREIRA1; DÉBORA DOLORES SOUZA DA SILVA2; ROSALI MARIA FERREIRA DA SILVA2; LARISSA ARAÚJO ROLIM3; PEDRO JOSÉ ROLIM NETO2. Centro Universitário Maurício de Nassau, Recife - PE - Brazil; 2Universidade Federal de Pernambuco, Recife - PE - Brazil; Universidade Federal do Vale do São Francisco, Petrolina - PE - Brazil.

1 3

Introduction and objective: Chagas’ disease is a public health problem for which the only available drug for treatment is benznidazole (BNZ), which has low solubility in water and high toxicity. Aiming to improve these characteristics, Metal Organic Framework (MOF) based systems have been developed, which allow the delivery of drugs, controlling their release kinetics. In order to characterize these systems, Differential Scanning Calorimetry (DSC) and Thermogravimetry (TG) are used aiming to obtain knowledge about its thermal degradation kinetics, a useful tool for the rational design of pharmaceutical products. Materials and methods: TG and DSC curves were performed using a Shimadzu® thermocouple/calorimeter model TGA Q60/ TA-60WS, respectively, under a flow of nitrogen atmosphere of 50 mL.min-1. The mass of the samples was about 3 mg (± 0.05) for the BNZ and ZIF-8 isolates and 6 mg (± 0.05) for the physical mixtures and system, placed on platinum crucible, in the temperature range of 25 to 1000 ° C under the heating rate 10 °C min-1. Results and conclusion: Through the TG and DSC curves it was possible to corroborate the fact that the system obtained in acetone made more efficient incorporation of the drug possible, either by the change of the thermal profile, or by the relative reduction of the mass loss presented by BNZ. Financial support or fellowship: CAPES; CNPq; FACEPE CFSP334 THREE-DIMENSIONAL VIEW OF CYCLO-OXYGENASE-2 INHIBITION BY INDOMETHACIN, A NON-STEROID ANTI-INFLAMMATORY MARLON MARCIO FERREIRA URATA; PATRICIA CARVALHO GARCIA; PAULA MARTINS DA SILVA; RENATO MASSAHARU HASSUNUMA. Universidade Paulista - UNIP, Bauru - SP - Brazil. Introduction and objective: According to the World Health Organization (WHO), non-steroidal anti-inflammatory drugs (NSAIDs) are the most prescribed analgesics for mild and moderate pain. NSAIDs such as Indomethacin (IMN) are non-selective, that is, they are able to inhibit both cyclooxygenase (COX) enzyme isoforms. In order to contribute to the current knowledge about the enzymatic inhibition of COX-2 by IMN, the present research has the objective to produce scripts in RasMol software for threedimensional visualization of the interactions between these substances. Materials and methods: Based on a selection of PDB files available at the Protein Data Bank site, the file 4COX.pdb, which presents the interaction of the COX-2 enzyme and the IMN, was selected for the development of scripts for RasMol 2.7.4.2 software for three-dimensional visualization of the active sites and amino acids involved in this process. Results and conclusion: The IMN is able to bind the active site of COX-2 in a relatively deep position. In the images produced in the RasMol from the scripts, it was possible to observe its position inside the hydrophobic channel of the COX-2. The chlorine atom of the IMN interacts with the leucine residue 384 (LEU384) that adopts a new conformation of its side chain due to this binding. The benzoyl group of the IMN is stabilized by hydrophobic interactions with LEU384, phenylalanine 381 (PHE381), tyrosine 385 (TYR385) and tryptophan 387 (TRP387). Interactions of IMN with serine 530 (SER530) and valine 349 (VAL349) were also observed. These interactions occur through the oxygen atom of the benzoyl group of the IMN with the hydroxyl of the side chain of SER530 and the side chain of VAL349. Therefore, biochemical and structural results obtained in the present study may be useful for future research about the inhibition of COX-2 by IMN, as well as for the development of new drugs.

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CFSP335 TOXIC EFFECT OF Α-L-FUCOSIDASE ADDED DURING IN VITRO FERTILIZATION OF BOVINE EMBRYOS RAYANE ISABELLE TURKÓCIO1; FERNANDA FAGALI FRANCHI2; EDUARDO MONTANARI RAZZA2; ANTHONY CÉSAR DE SOUZA CASTILHO3; PATRÍCIA KUBO FONTES1. Universidade Paulista, Bauru - SP - Brazil; 2Universidade Estadual Paulista, Botucatu - SP - Brazil; 3Universidade do Oeste Paulista, Presidente Prudente - SP - Brazil. 1

Introduction and objective: Brazil is the largest in vitro embryo producer (IVEP) in the world, holding 70% of world production. The in vitro production (IVP) of bovine embryos is composed of three steps: oocyte in vitro maturation, in vitro fertilization and in vitro culture, with a blastocyst production success rate of about 40% or less. The scientific community goal is to improve the blastocyst production rate, improving embryo quality for increasing the pregnancy rate. To simulate the reproductive physiology research studying different culture media to the technical improvement. A-L-Fucosidase (FUCA) is a protein present in the oviduct, where the fertilization occurs. Because of that, the present work aimed to test the addition of FUCA during in vitro fertilization, to analyze the benefits of this protein in embryo production rate and to increase the quality of embryos produced in vitro. Materials and methods: Experimental procedures were approved by the Ethics and Animal Handling Committee from the Universidade Paulista. Ovaries from a local abattoir were collected. The cumulus-oocyte complex (female gamete) were obtained from these ovaries, selected and matured for 24 hours. After maturation, was proceeded to in vitro fertilization, divided into two groups: control group (18h in fertilization medium without FUCA) and FUCA group (18h in the middle of fertilization FUCA, 0.125 UI/mL of FUCA). And finally, culture for 7 days to blastocyst production. Results and conclusion: The control group had standard rate of in vitro embryo production (~ 40%). However, the addition of FUCA was harmful to the embryos, 100% of degenerate structures. We conclude that the FUCA had adverse effects on the gametes. It proved to be toxic in embryo production. Financial support or fellowship: FAPESP (Grant #2013/11480-3). CFSP336 USE OF THE KYOTO ENCYCLOPEDIA OF GENES AND GENOMES (KEGG) DATABASE FOR BACTERIAL CULTURE OPTIMIZATION SIBYLLE SOPHIE HACKER1; PROF. DR. MARCO ANTÔNIO STEPHANO2. Faculdade de Ciênicas Farmaceuticas da Universidade de São Paulo, São Paulo - SP - Brazil; 2Faculdade de Ciênicas Farmacêuticas da Universidade de São Paulo, São Paulo - SP - Brazil. 1

Introduction and objective: Bacterial species requires different growth conditions and media composition for propagation. KEGG is a resource for understanding functions and utilities at a molecular-level and can be used to optimize the analyses of metabolic flux from bacteria. Materials and methods: We analyzed the growth in two different culture media (Brain Heart Infusion - BHI and Trypticase soy broth - TSB) and with three different supplements (L-Proline. Nicotic Acid and Inositol). Comparative studies showed that the use of L-Proline and Inosine was more effective for microrganisms grown and analyzing the metabolic flux from KEGG database we can explain that L-Proline and Inosine is more converging metabolic pathways. And the use of combination of L_Proline, Inosine and Nicotic Acid, the microorganisms present the worst grown, probably because the metabolic pathway is diverging. Connecting the data types, it is possible to get a better understanding how the different metabolic fluxes correlate with other and how the factors and mechanisms of growth rate can be designed in the planning and design phase using the Kegg database. Results and conclusion: The nutritional medium provided the supplements to provide the other missing precursors. Glucose as energy source is more abundant and normally more used in a defined medium source and directly influences bacterial growth and cell concentration because highly reduced. Financial support or fellowship: CAPES

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CFSP337 VALIDATION OF KINETIC CHROMOGENIC METHOD FOR BACTERIAL ENDOTOXIN DETERMINATION IN HEMODIALYSIS WATER ELLEN GAMEIRO HILINSKI 1; FERNANDO PONTES DE LIMA E SILVA 1; ADRIANA APARECIDA BUZZO ALMODOVAR1; TEREZINHA DE JESUS ANDREOLI PINTO2; ADRIANA BUGNO1. Instituto Adolfo Lutz, São Paulo - SP - Brazil; 2Universidade de São Paulo, São Paulo - SP - Brazil.

Introduction and objective: The endotoxin level present in hemodialysis water is an important quality indicator since high concentrations of endotoxin are the main source of chronic inflammation in patients undergoing dialysis. This study aims to validate the analytical method for the quantitative determination of bacterial endotoxin in hemodialysis water samples by the kinetic chromogenic method and to compare the results obtained with the gel coagulation method (qualitative). Materials and methods: Sensitivity confirmation tests and interferences test in gel coagulation method were performed according to the Brazilian Pharmacopoeia (2010) in three hemodialysis water samples (dilution 1: 2 v/v) with previously negative results for endotoxin. The validation of the kinetic chromogenic method was accomplished using the same samples (1: 2 dilution v/v), in duplicate, through the portable test system (PTS®). Results and conclusion: The geometric means of the final point concentrations obtained in the LAL sensitivity confirmation tests and in the interference test by gel coagulation method presented a result of 0.125 EU/mL (λ) for the three samples evaluated. The results obtained for the recovery of the positive control of the product varied between 89 and 189% and the coefficient of variation between the samples ranged from 2.5 to 18.2%, demonstrating that samples did not show interference against the conditions of the test by the chromogenic method. The data demonstrated that the samples evaluated presented equivalent results in both methods. Therefore, the chromogenic method was validated for the 1: 2 dilution of the sample, which allows the implementation of the test in the public health laboratories.

VASORELAXANT EFFECTS OF TAURINE ON AORTA FROM CONTROL AND OBESE RATS

CFSP338

CINTHYA MACIEL DE MELO; VALÉRIA DE FÁTIMA LEÃO ; LETICIA LIMA DIAS MOREIRA FERREIRA; ROSANE APARECIDA RIBEIRO; JULIANA MONTANI RAIMUNDO. Universidade Federal do Rio de Janeiro - Campus Macaé, Rio de Janeiro, Rj, Brazil, Macae - RJ - Brazil. Introduction and objective: Taurine (Tau) is an amino acid involved in the regulation of cardiovascular function. Studies on the vasodilator effect of Tau are controversial and it has not been evaluated in vessels from obese animals yet. This work evaluated the mechanisms involved in Tau-induced vasodilation in aortas from control and obese Wistar rats. Materials and methods: Male Wistar rats received subcutaneous injections of monosodium glutamate (MSG; 4g/kg body weight) or saline (control; 1.25 g/kg body weight) during the first 5 days of life. At 150 days of age, aortic rings were prepared for isometric tension recording and the contractile response to phenylephrine (10 μM) was measured before and after exposure to cumulative concentrations of Tau (20-80 mM). To investigate the mechanism of action, aortas with endothelium were pretreated with L-NAME (100 μM; inhibitor of nitric oxide synthase) or TEA (10 μM; non-selective blocker of K+ channels). The local Animal Care and Use Committee approved all protocols (License MAC003). Results and conclusion: Obese rats presented higher perigonadal and retroperitoneal fat stores compared to control rats and showed endothelial dysfunction. At 80 mM, Tau induced vascular relaxation of 20.2 ± 1.7 % (P <0.05) and 10.9 ± 2.4 % (P <0.05) in control and MSG aortas, respectively. In the presence of L-NAME and TEA, Tau-induced vasodilation in control aortas was partially reduced. On the other hand, TEA did not alter the effect of Tau on MSG aortas. Conclusion: The vasorelaxant effect of Tau involves the production of NO and the activation of K+ channles. However, the effect of Tau is reduced in aortas from obese rats. Financial support or fellowship: FAPERJ; CAPES; CNPq.

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CFSP339 X-RAY DIFFRACTION STUDIES FROM CAPSULAR POLYSACCHARIDE OF GROUP B STREPTOCOCCI SIBYLLE SOPHIE HACKER1; DR. ANTÔNIO CARLOS FRANCO DA SILVEIRA2; PROF. DR. MARCO ANTÔNIO STEPHANO3. Faculdade de Ciênicas Farmaceuticas da Universidade de São Paulo, São Paulo - SP - Brazil; 2Instituto de Fisica da Universidade de São Paulo, São Paulo - SP - Brazil; 3Faculdade de Ciencias Farmaceuticas da Universidade de São Paulo, São Paulo - SP - Brazil. 1

Introduction and objective: Polysaccharide capsules are complex carbohydrates of many monosaccharides joined together by glycosidic bonds. Bacterial capsular polysaccharides are important virulence factors of pathogenesis. Capsules protect the bacteria against phagocyte killing, prevent desiccation and have antiadherent properties. As the main virulence factor, polysaccharide vaccines are important for protection against infection. For this reason, understanding all physical chemical properties is very important. Materials and methods: The biological properties of polysaccharides are related to their composition and structure. Many factors, such as sugar, binding, molecular weight or biopolymer sulfate content, influence the relationship between structure and function. The analysis of X-ray diffraction is a useful tool for preliminary identification of the dimensional structure of polysaccharides at the molecular level and characterize a large number of functional groups. This technique allowed us to distinguish between the repeating units. Thus, the study of X-ray diffraction and its analysis on composition is important in order to plan the best method for conjugation with a protein. For crystalline materials, X-ray diffraction analysis characterizes the different phases and collaborates to identify the chemical components and the morphological structure through the identification of the interplanar distances. We conducted several analyses in order to analyze the importance of making a good preparation and experimental planning of material. Results and conclusion: Buffer conditions and pH can affect polysaccharide purification and consequently the physical chemical characterization. The purification stage is very important and essential in biological products because it determines the yield, product consistency and reproducibility. The purification process influences the structures, residue effects of solution ionic strength on physical properties. Financial support or fellowship: Financial support from CAPES. The X- Ray measurements were performed in the Crystallographic Unit of the Applied Physical Laboratory at the Faculty of Physics of the University of São Paulo

Brazilian Journal of Pharmaceutical Sciences

Area 3 Clinical and Toxicological Analyzes

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

ALTERATION OF SPERMATOGENESIS IN PUBERTAL MICE TREATED WITH ISOTRETINOIN

185

CFSP340

EDUARDA VICENTINI MENEZES; ANA CLARA CERATO BISPO; TALITA CRISTINA GALVÃO; VIVIAN MAYUMI TAMOYESE; GABRIEL ARAUJO COSTA; SOLANGE DE PAULA RAMOS; MARIA JOSÉ SPARÇA SALLES. Universidade Estadual de Londrina, Londrina - PR - Brazil. Introduction and objective: Isotretinoin (Roacutan®), a derivative of vitamin A, is used to treat severe acne, principally affecting adolescents. The objective was to analyze spermatogenesis in pubertal mice treated with isotretinoin. Materials and methods: This study was approved by the Ethics Committee on Animal Experimentation of UEL, nº 2919201779/2017. Pubertal male mice were divided into 2 experimental groups: The animals of the treated group (G1) received isotretinoin at a concentration of 1 mg/kg/day via gavage for 45 days, and the control group (G0) received the equivalent of the excipient in vegetable oil. On day 45 the animals were euthanized. Organs such as the epididymis, testicles, and seminal vesicles were weighed and the testosterone dosed. The morphology of spermatozoa was analyzed, and Johnsen’s protocol (1970) used for histological analysis of the testicles. Results and conclusion: The treated group presented statistically significant results, such as weight reduction of the epididymis (G0 = 0.091g ± 0.002 and G1 =0.080g ± 0.002), testicles (G0=0.211g ± 0.009 and G1= 0.199g ± 0.010), seminal vesicle (G0 = 0.222g ± 0.015 and G1 =0.176g ± 0.013), and low testosterone level (G0= 2.423ng/mL ± 0.592 and G1 = 2.162ng/mL ± 0.464). The Johnsen score was lower when compared to the controls, as there was a higher prevalence of immature seminiferous tubules in the treated mice, with incomplete germ cell lineage (G0= 9.28 and G1= 8.47). Analysis of sperm morphology demonstrated alterations in the tails (G0= 21.17% and G1=22.57%) and heads (G0 =4.94% and G1 = 6.60%). Exposure to the drug caused damage to spermatogenesis. The information obtained could contribute to healthcare programs in the population of reproductive age, making possible prevention strategies against possible damage. Financial support or fellowship: CNPq. CFSP341 ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES OF ETHYL ACETATE AND BUTANOLIC FRACTIONS OF VITEX POLYGAMA LEAVES RAISSA GABRIELA MENEZES DOS SANTOS PONTES; RENATA JESUS MELLO; CRISTIANE CATELA JESUS; MICHELLE FRAZÃO MUZITANO; JULIANA MONTANI RAIMUNDO; PAULA LIMA DO CARMO. UFRJ, Macaé - RJ - BRAZIL. Introduction and objective: We previously demonstrated the antinociceptive effect of the ethyl acetate (EAF) and butanolic (BF) fractions of the leaves of Vitex polygama. The objective of this study was to evaluate the dose-response effect of these fractions. Materials and methods: The experiments were performed on male Swiss mice (18-25 g, n=8-10). The fractions were administered i.p., 30 min before the tests. Protocols (approved by CEUA/Macaé - MAC038): 1) Abdominal writhing test: acetic acid was injected (0.8% v/v, 10 ml/kg, i.p.) and the number of writhes was quantified for 10 min. 2) Formalin test: formalin was administered (2.5%, 20 μl) in the right hind paw intraplantar region, and quantified by the number of licks to the paw after: 0-5 min (neurogenic phase) and 15-30 min (inflammatory phase). 3) Hot plate: the animals were placed on the hot plate (54 °C) prior to injection and 30, 60, 90 and 120 min after injection of fractions, and the time without licking the legs was measured. Results and conclusion: 1) EAF reduced the number of abdominal writhes from 30.0±2.9 (DMSO) to 19.5±1.1, 17.2±1.5, and 4.5±1.5 (P<0.05) and BF to 15.2±2.1, 7.4±0.7 and 5.2±1.4 (P<0.05) at 10, 20 and 30 mg/kg, respectively. 2) EAF reduced the reaction time in the neurogenic phase of 52.3±6.6s (DMSO) to 36.2±2.0, 34.6±2.2 and 25.6±3.1s (P<0.05); and BF to 35.6±3.0, 27.1±1.2 and 26.7±2.2s (P<0.05) at 10, 20 and 30 mg/kg, respectively. In the inflammatory phase, EAF reduced the reaction time from 345.7±20.7s (DMSO) to 203.6±18.0, 210.4±4.9 and 204.0±10.8 s (P<0.05); and BF to 340.8±14.2, 280.5±10.9 (P<0.05) and 227.6±7.3s (P<0,05) at 10, 20 and 30 mg/kg, respectively. 3) EAF or BF did not change the residence time on the hot plate at any of the evaluated times. The antinociceptive effect of EAF and BF is mediated by a peripheral anti-inflammatory action. Financial support or fellowship: FAPERJ.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

ANALYSIS OF INORGANIC COMPONENTS IN CLOVE OIL

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CFSP342

ANDERSON ROSSATTO; KARINE DUREGGER. Faculdades Metropolitanas Unidas, São Paulo - SP - Brazil. Introduction and objective: Essential oils can be obtained by different extraction techniques, such as hydro distillation, steam distillation, extraction by organic solvents and with supercritical fluid, with steam-drawn distillation being the most commonly used. Considering the widespread use of these oils in several areas of the pharmaceutical field, easy acquisition and the lack of a strict control on the commercialization of these products, the evaluation of the concentration of these elements is of fundamental importance for the health of the population. Materials and methods: Levels of Minerals were analyzed in essential clove oils, by Energy Dispersive X-rays fluorescence (EDXRF). In the study, a pipette between 50 µL and 100µL of samples, was packed in a port sample specific and measured in vacuum in spectrometer EDX720- Shimadzu co (15~50kV and 50µA). The quantification was taken by fundamental parameters method and multivariate calibration used to correct effects of the matrix. Results and conclusion: For the technique applied, significant values from potentially toxic or contaminant elements were not observed. Financial support or fellowship: Instituto de Pesquisas Energéticas IPEN/USP CFSP343 ANTIMICROBIAL ACTIVITY AND ERYTHROCYTARY OSMOTIC FRAGILITY OF THE DRY GROSS EXTRACT FROM THE LEAVES OF THE SOLANUM PANICULATUM L. (JURUBEBA) JOICE LUIZA PEREIRA DA SILVA; IZABELLA CINTHIA TÔRRES DE VASCONCELOS; MARIA ADRIANA FERREIRA FARIAS; MARIA GABRIELLE DE OLIVEIRA TABOSA; RISONILDO PEREIRA CORDEIRO. ASCES - UNITA, Caruaru - PE - Brazil. Introduction and objective: The Solanum paniculatum L. has multiple properties such as antioxidant and molluscicide, attenuating its therapeutic applicability. In this way, the aim was to analyze the antimicrobial activity starting from the Minimum Inhibitory Concentration (MIC) and the Minimum Inhibitory Concentration of Adherence (MICA) and also the Erythrocytary Osmotic Fragility from the dry gross extract of leaves from the Jurubeba. Materials and methods: The strains tested by the antimicrobial analysis were: Salmonella, E. coli, S. aureus, P. aeruginosa, S. agalactiae e C. albicans. To determine the MIC, the wells technique was used, starting with dilutions at 50%, 25%,12.50% and 6.25% with respect to the initial sample of the extract (0.5g). The plates were incubated at 37°C for 20 hours for results analysis. For control the antibiotic amoxicillin was used. The MICA was determined by the technique of the sloping tube in the presence of sucrose at the same conditions as the MIC. In the Erythrocytary Osmotic Fragility analysis 50mg of the extract was diluted at concentrations of 1000, 750, 500, 250, 100 and 50 µL/mL in tubes containing 5 mL of NaCl 0.9%. In each tube 25 µL of sheep’s blood was added and then it was subjected to centrifugation, the absorbance was read in spectrophotometer. Results and conclusion: The extract was positive against the strains S. aureus, P. aeruginosa, S. agalactiae and E. coli only in the concentration of 50% showing the formation of halo and preventing the production of biofilm. As for the Erythrocytary Osmotic Fragility, the extract presented hemolysis of 20.45% in a concentration of 1000 µL/mL, resulting in a moderate percentage of the lysis of red blood cells in front of the tested parameters. It is concluded that its leaves have antibiotic capacity and also have the potential to produce new medicines. Financial support or fellowship: ASCES -UNITA

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

ANTIMICROBIAL ACTIVITY OF THE STEM OF CNIDOSCOLUS QUERCIFOLIUS POHL.

187

CFSP344

IRTHYLLA NAYALLE DA SILVA MUNIZ1; ALANE ALEXANDRA DA SILVA OLIVEIRA2; BRUNNA LARISSA DE SOUZA MELO FERREIRA2; MARIA ADRIANA FERREIRA FARIAS3; SANDRIEL FELIPE MACEDO DE OLIVEIRA3; ARQUIMEDES FERNANDES MONTEIRO DE MELO3. ASCES/UNITA, Caruaru - PE - Brazil; 2ASCES - UNITA, -; 3ASCES - UNITA, Caruaru - PE - Brazil.

Introduction and objective: The Cnidoscolus quercifolius Pohl, is a species of the caatinga biome, popularly known as favela and also belongs to the Euphorbiace Family. It represents a contribution for the discovery of new therapeutic agents in the treatment of diseases caused by multiresistant microorganisms. This paper aimed to determine the Minimum Inhibitory Concentration (MIC) and the Minimum Inhibitory Concentration of Adherence (MICA) of the Cnidoscolus quercifolius Pohl. Materials and methods: Strains of: Escherichia coli, Staphylococcus aureus, Candida albicans, Klebsiella, Lactobacillus acidophilus, Staphylococcus epidermidis, and Pseudomonas aeruginosa were used. To determine the MIC the wells technique was used, from dilutions of 50%, 25%, 12.50% and 6.25% in relation with the extract’s initial sample (0.5g). The plates were incubated at 37°C for 18 hours for analysis of the results. Amoxicillin antibiotic was used for control. The MICA was determined by the technique of the inclined tube in the presence of sucrose in the same conditions as the MIC. Results and conclusion: The Stem of Cnidoscolus quercifolius Pohl presented a positive result on the MIC in concentration of 25% at the L. acidophilus, C. albicans, E. coli, S. epidermidis and Klebsiella strains. The MICA tests performed with the extract of the Stem was negative with biofilm formation. It can be concluded that this plant is viable for future scientific studies about its antimicrobial potential aiming at production of antibiotics. Financial support or fellowship: ASCES -UNITA CFSP345 ANTINOCEPTIVE ACTIVITY OF HYDROALCOOLIC EXTRACT AND FRACTIONS OF THE LEAVES OF MANDEVILLA MORICANDIANA (APOCYNACEAE) SOLEANE APARECIDA SOUZA GONÇALVES MACIEL1; RAISSA GABRIELA MENEZES DOS SANTOS PONTES2; LETICIA LIMA DIAS MOREIRA FERREIRA2; IVANA CORREA LEAL3; MICHELLE FRAZÃO MUZITANO2; JULIANA MONTANI RAIMUNDO4; PAULA LIMA DO CARMO2. UFRJ- Macae, São Pedro da Aldeia - RJ - Brazil; 2UFRJ-Macaé, Macaé - RJ - Brazil; 3UFRJ- Macaé, Macae - RJ - Brazil; UFRJ- Macae, Macaé - RJ - Brazil.

1 4

Introduction and objective: Several species of the family APOCYNACEAE have had their analgesic activity described. However, this effect has not been investigated for Mandevilla moricandiana. The objective was to evaluate the antinociceptive activity of the hydroalcoholic extract (HE) and the fractions of the leaves of M. moricandiana. Materials and methods: The experiments were performed on male Swiss mice (18-25g, n=6-10). HE and fractions were administered i.p., 30 min before the tests. Protocols (approved by CEUA/Macaé - MAC038): 1) Abdominal writhing test: acetic acid was injected (0.8% v/v, 10 ml/kg, i.p.) and the number of writhes was quantified for 10 min. 2) Formalin test: Formalin was administered (2.5%, 20μl) in the right hind paw intraplantar region, and quantified by the number of licks in the paw after: 0-5 min (neurogenic phase) and 15-30 min (inflammatory phase). Results and conclusion: 1) Abdominal writhing test: the HE reduced the number of abdominal writhes from 21.2±1.5 (DMSO) to 8.6±4.4. Results and conclusion: 1) HF reduced the number of abdominal writhes from 27.2±2.9 (DMSO) to 7.7±1.9 and 7.1±1.6 Financial support or fellowship: FAPERJ.

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CFSP346 ANTINOCICEPTIVE ACTIVITY OF HEXANIC AND DICHLOROMETHANIC FRACTIONS OF PASSIFLORA MUCRONATA LEAVES. RAISSA GABRIELA MENEZES DOS SANTOS PONTES1; SOLEANE APARECIDA SOUZA GONÇALVES MACIEL1; ISABEL CRISTINA VIEIRA SILVA2; IVANA CORREA LEAL2; MICHELLE FRAZÃO MUZITANO1; JULIANA MONTANI RAIMUNDO1; PAULA LIMA DO CARMO1. UFRJ, Macaé - RJ - Brazil; 2UFRJ, Rio de Janeiro - RJ - Brazil.

Introduction and objective: We have previously shown that the hexane (HF) and dichloromethane (DF) fractions of Passiflora mucronata leaves have antinociceptive activity in the abdominal writhing test. The objective of this study was to evaluate the dose-response effect of these fractions. Materials and methods: The experiments were performed on male Swiss mice (18-25 g, n= 6-10). The fractions were administered i.p., 30 min before the tests. Protocols (approved by CEUA/Macaé - MAC038): 1) Abdominal writhing test: acetic acid was injected (0.8% v/v, 10 ml/kg, i.p.) and the number of writhes was quantified for 10 min. 2) Formalin test: Formalin was administered (2.5%, 20 μl) in the right hind paw intraplantar region, and quantified by the number of licks to the paw after: 0-5 min (neurogenic phase) and 15-30 min (inflammatory phase). Results and conclusion: 1) HF reduced the number of abdominal writhes from 27.2±2.9 (DMSO) to 7.7±1.9 and 7.1±1.6 (P<0.05) at 10 and 20 mg/kg, respectively; and DF reduced to 15.8±2.3; 10.8±3.3 and 2.8±1.1 (P><0.05) at 2.5; 5 and 10 mg/kg, respectively. 2) HF reduced the reaction time in the neurogenic phase of 52.3±6.6 s (DMSO) to 29.4±3.3, 24.33±1.6 and 26.43±0.8 s (P><0.05) at 5, 10 and 20 mg/kg, respectively; and DF to 27.2±4.1 s (P><0.05) at 10 mg/kg. In the inflammatory phase, HF reduced the reaction time from 345.7±20.7 s (DMSO) to 210.9±25.3, 197.1±21.95 and 168.2±21.7 s (P><0.05) at 5, 10 and 20 mg/kg, respectively; and DF to 250.1±22.1 and 208.2±16.7 s (P><0.05) at 5 and 10 mg/kg, respectively. These results show the dose-dependent antinociceptive action of the fractions of P. mucronata. Financial support or fellowship: FAPERJ. CFSP347 APPLICATION OF THE VITREOUS HUMOR IN POST-MORTEM ANALYSES TO DETECT DRUGS OF ABUSE: A STATE-OF-THE-ART REPORT RADIR MENDES CARNEIRO; ÉRICA PENTEADO; PATRÍCIA DA FONSECA LEITE. Universidade Paulista, Campinas - SP - Brazil. Introduction and objective: The post-mortem analysis to detect drugs of abuse can be quite complex, or even impossible to be performed, due to high degradation of the traditional biological matrices (e.g., blood, urine and stomach contents). Therefore, in recent years it has been sought to employ alternative matrices for detecting drugs of abuse, such as hair, nail, skin and vitreous humor. Among these matrices, Vitreous Humor (VH) is a privileged matrix, as it is easy to collect, is located in the human body in an isolated compartment (fills the space between the lens and the retina of the eyeball), relatively protected from external contamination and general traumas. The objective of this work is to present a literature review on the use of the VH as matrix in post-mortem analyses aimed at detecting and quantifying drugs of abuse. In particular, the collection and storage conditions, sample preparation and analytical techniques, as well as the interpretation of results from qualitative and quantitative points of view are dealt with. Materials and methods: A rigorous bibliographical review was performed. For this purpose, a survey was conducted of publications found in the Medline/PubMed, Scielo, Science Direct, Springer and other databases from 1990 until 2016. Results and conclusion: Analysis of cocaine and its derivatives, benzodiazepines, barbiturates, amphetamine, cannabinoids and ethanol are good examples, illustrating the whole range of analytical techniques that have been implemented for VH analysis – using, for instance, gas chromatography (GC), high-performance liquid chromatography (HPLC), GC/mass spectrometry (MS) and immunoanalysis. The VH is an alternative matrix useful for forensic toxicology, recommended especially in cases of body injuries, partial carbonization or in a state of decomposition. However, the limitations of VH for the purpose of forensic toxicology largely concern quantitative interpretation – some studies show that VH and blood concentrations do not correlate for all compounds. To clarify the quantitative interpretation, new research is necessary to respond to this important gap – in particular, to expand our knowledge concerning the distribution of drugs from blood.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

ASSOCIATION BETWEEN ABCB1 C.3435C>T GENOTYPES AND STABLE WARFARIN DOSES

189

CFSP348

LETÍCIA CAMARGO TAVARES1; LEILIANE RODRIGUES MARCATTO1; RENATA ALONSO GADI SOARES1; CELIA MARIA CASSARO STRUNZ1; MAURICIO IBRAHIM SCANAVACCA1; JOSÉ EDUARDO KRIEGER1; ALEXANDRE DA COSTA PEREIRA1; PAULO CALEB JUNIOR LIMA SANTOS2. Incor, São Paulo - SP - Brazil; 2Unifesp, São Paulo - SP - Brazil.

Introduction and objective: In the last 65 years, warfarin has been the most widely prescribed oral anticoagulant for treating and preventing thromboembolic disorders. However, due to its narrow therapeutic index and high variance of pharmacokinetics and pharmacodynamics, warfarin interpatient responses vary widely. Besides demographic and clinical factors, genetic factors are well established as influencing the stable dose of warfarin therapy. One of the genetic variants that may be a determinant of warfarin response variability is the ABCB1 gene, which codes for the known multidrug efflux pump P-glycoprotein (P-gp). The aim of this study was to assess the association of the ABCB1 c.3435C>T (rs1045642) genotypes with required doses of warfarin. Materials and methods: For this retrospective study, DNA samples from 1,028 patients, enrolled in the Arrhythmia and Pacemaker Clinical Units of Heart Institute (InCor), University of São Paulo Medical School (FMUSP), were genotyped for ABCB1 c.3435C>T by polymerase chain reaction followed by melting curve analysis (HRM-PCR). Of the included patients, 309 presented stable warfarin maintenance dose (when the three evaluated consecutives measures of standardized prothrombin time (INR) reached the target range 1.8 to 3.2). Results and conclusion: The observed genotypic distribution for ABCB1 c.3435C>T polymorphism were: 35.0% CC wild-type, 45.7% CT heterozygous-type, and 19.3% TT homozygous-type (n=1,028). For the sample of patients with stable warfarin treatment (n=309), the observed required dose of warfarin ranged from 6 to 70 mg per week (median = 28). By performing a univariate analysis of variance, we found different mean dose according with ABCB1 c.3435C>T genotypes: CC=31 ± 1, n=98; CT=28 ± 1, n=153; TT=26 ± 1, n=58; p=0.014; mean stable dose ± SD (mg/week). The data were adjusted for age, gender, body mass index (BMI), self-reported race, use of amiodarone, CYP2C9 (*2 and *3), and VKORC1 c.1639G>A. In conclusion, our results suggest ABCB1 c.3435C>T polymorphism may have a potent impact on warfarin required dose variability, when associated with other genotypic, demographic and clinical factors. Financial support or fellowship: Financial Support: São Paulo Research Foundation (FAPESP), Process Number: 2013/09295-3. This study was approved by the ethics committee and all subjects included provided written informed consent (SDC: 4519/17/019, CAPPesq: 0804/10).

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CFSP349 ASSOCIATIONS BETWEEN BIOCHEMICAL PARAMETERS OF PATIENTS WITH ADVANCED CHRONIC KIDNEY DISEASE HELLIDA DA COSTA ALVES TEIXEIRA; JOSILENE DÁLIA ALVES; FERNANDA REGINA CASAGRANDE GIACHINI VITORINO; VICTOR VITORINO LIMA. Universidade Federal de Mato Grosso, Barra do Garças - MT - Brazil. Introduction and objective: Chronic kidney disease (CKD) has increased worldwide to epidemic level. Due to the importance of this pathology, these patients should be continuously monitored, and preventive and therapeutic measures should be implemented as soon as the CKD was diagnosed. Objective: Evaluate the biochemical profile of chronic renal patients in advanced stages, by performing tests of urea, phosphorus, potassium, calcium and identifying correlations and regressions between the factors analyzed. Materials and methods: 71 patients with CKD stages 3, 4 and 5 and 21 control were studied. Blood sampling of the patients was taken to determine the serum levels of urea, potassium, phosphorus and calcium. Statistical analysis was performed using ANOVA followed by Tukey post test for quantitative data and for the categorical variables we used the chi-square test. Pearson correlation analysis and linear regression were also performed. Results and conclusion: The majority of participants were male, their mean age was 60.01 (± 16.15) years old; the serum level of urea, potassium and phosphorus increased as chronic kidney disease progresses, and the stage 5 patients had serum levels of the same factors higher than control group and the other stages of CKD. It was not possible to observe significant changes between the study groups in terms of serum calcium levels. The correlation between urea and phosphorus in CKD patients showed as significant, the value of r = 0.749 (p = 0.00), which consists of a strong correlation and the linear regression was 63.5% (p=0.02). A moderate correlation between the serum of urea and potassium in patients with CKD, was observed with r = 0.671 (p = 0.00) and the linear regression was 50.2% (p=0.00). While the correlation between phosphorus and potassium is moderate, with r = 0.504 (p = 0.00) the linear regression was 25.4% (p=0.00). Patients with CKD in advanced stages have higher levels of urea, potassium and phosphorus and these biochemical factors are directly correlated. These abnormalities may contribute to the occurrence of cardiovascular diseases, since cardiovascular disease is the leading cause of death in this population. Financial support or fellowship: CNPq; CAPES; FAPEMAT;

BIOCHEMICAL ANALYSIS OF WOMEN DEPRIVED OF LIBERTY IN SANTARÉM, PARÁ

CFSP350

NÁDIA VICÊNCIA DO NASCIMENTO MARTINS1; LUCIA YASUKO IZUMI NICHIATA2; DEBORA CAROLINA MARTINS CORRÊA3; JÉSSICA SAMARA DOS SANTOS OLIVEIRA4; LAYS OLIVEIRA BEZERRA4; RITA DE CÁSSIA BATISTA DA SILVA3; ANDREW MAIROM NOGUEIRA PEREIRA3; GEOVANI WANGHAN FIGUEIRA3. Universidade do Estado do Pará/Escola de Enfermagem USP, Santarém - PA - Brazil; 2Escola de Enfermagem da Universidade de São Paulo, São Paulo - SP - Brazil; 3Instituto Esperança de Ensino Superior, Santarém - PA - Brazil; 4Universidade do Estado do Pará, Santarém - PA - Brazil. 1

Introduction and objective: The health of the population of women deprived of liberty include those in the context of vulnerability, and precarious conditions of housing, health, food and violence predispose them to numerous health problems, including dyslipidemias, hypertension, diabetes mellitus and cardiovascular diseases. Analyze the biochemical examinations of women deprived of their liberty in Santarém, Pará. Materials and methods: Materials and methods: Exploratory, descriptive, cross-sectional, quantitative study conducted with 54 women deprived of their liberty, in the municipality of Santarém, State of Pará. Results and conclusion: It was observed that 33.3% of the women were between the ages of 18 to 23 years, followed by 25.9% in the age range of 24 to 29 years. As to skin color of the participants, it was found that 79.6 percent of women declared themselves as Brown. Related to Glycemic Indexes, it was noticed that 96.2% of women showed results consistent with the normal Glycemic (≤ 65 99 mg/dL), with 1.9% with 100 mg/dL and with only 1.9% 276 mg/dL, as high value. As for cholesterol, 37.5% was proven with border values of cholesterol (200-239 mg/dL), and 43.8% of women showed high cholesterol (≥ 240 mg/dL). In relation to the triglycerides, it was found that 54.2% of the sample was with the desirable level (< 150 mg/dL), 25% borderline (150-200 mg/dL), 20.8% high (200-499 mg/dL). The biochemical tests performed present significant changes, in most cases, the dosage of cholesterol and triglycerides. So, it is necessary to promote studies directed at this population to show the possibility for improving quality of life in prison. Financial support or fellowship: University of the State of Pará Santarém, Pará.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

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CFSP351 CALCULATION OF THE YIELD AND ANTIMICROBIAL ACTIVITY OF THE DRY GROSS EXTRACT FROM THE FRUITS OF THE SOLANUM PANICULATUM L. (JURUBEBA) JOICE LUIZA PEREIRA DA SILVA1; IZABELLA CINTHIA TÔRRES DE VASCONCELOS2; JAMICELLY RAYANNA GOMES DA SILVA2; VANESSA CAMYLLA BERNARDO DE OLIVEIRA2; CAMILLA ISABELLA FERREIRA SILVA2; JULIANNE BEZERRA REGIS DE CARVALHO2; ANALÚCIA GUEDES SILVEIRA CABRAL2. ASCES - UNITA, Caruaru - Brazil; 2ASCES - UNITA, Caruaru - PE - Brazil.

Introduction and objective: The Jurubeba’s fruit has some medicinal attributes, such as uses for treatment of Chronic hepatologies. This study aimed to analyze the yield and the antimicrobial activity against Minimum Inhibitory Concentration (MIC) and the Minimum Inhibitory Concentration of Adherence (MICA) of the Extract from its fruits. Materials and methods: The total collected and weighed was 561 g, which was the crushed and macerated for 7 days in a Hydroalcoholic extractive solution 95% (v/v). After this procedure, the extrusion and evaporation of the extractive solution were performed until the loss of 95% of its humidity. The extract was put in B.O.D. to obtain the Dry Gross Extract, resulting in 41.03 g. The yield of this Extract was calculated with the formula: Yield= (Weight of the Extract/Weight of the fresh fruits) x 100. The tested strains for microbial analysis were: Salmonella, E. coli, S. aureus, P. aeruginosa, and C. albicans. For the MIC, the wells technique was used, with dilutions of 50%, 25%,12.50% and 6.25%, in relation to the initial Dry Gross Extract sample (0.5g). The plates were incubated at 37°C for 20 hours for results analysis, using amoxicillin as control. The MICA was determined by the technique of the inclined tube in the presence of sucrose in the same conditions as the MIC. Results and conclusion: The obtained result of the yield was 7.31%, which demonstrated enough assiduity for the realization of further tests with the obtained extract. The extract from the jurubeba’s fruit didn’t present antimicrobial activity at the applied methodologies against the tested strains, which proves that its fruits are not of interest for the production of possible antimicrobial studies addressed to the bacteria used. Financial support or fellowship: ASCES -UNITA CFSP352 CLASSIFICATION OF RESISTANCE PHENOTYPES IN THE ENTEROBACTERIA RESISTANT TO CARBAPENEMS ISOLATED FROM PATIENTS OF A PUBLIC HOSPITAL IN THE MUNICIPALITY OF BAURU-SP DEVLYN PICOLOTO SHIL; ADRIANA APARECIDA FELTRIN CORREA. UNIP Bauru, Bauru - SP - Brazil. Introduction and objective: Nowadays, the presence of isolates of carbapenem-resistant enterobacteria (CRE) in public hospitals in the city of Bauru-SP is observed. No phenotype classification for this resistance is available currently. The aim of this study is to classify bacterial strains with CRE phenotype isolated from a public hospital in the city of Bauru-SP. Materials and methods: Patients that acquired strains of CRE during the year 2016 were included. The isolated strains were identified and tested for antimicrobial susceptibility by automated technique (Biomerieux-Vitek2TM) and resistance confirmation was performed by Biomerieux-Etest TM. The identification of the resistance phenotype followed ANVISA-Technical Note nº01/2013 after recovery of the frozen strains. Results and conclusion: The tests were performed in 79 samples, of which 44 (55.7 %) represented surveillance cultures and 35 (44.3 %) clinical cultures. Samples from the clinical cultures were isolated from blood cultures (5; 14.3 %), cerebrospinal fluid (2; 5.7 %), catheter tips (2; 5.7 %), tracheal secretions (4; 11.4 %) and urocultures (22; 62.9 %). Among the surveillance samples, 41 (93.2%) were isolated from the ICU and 3 (6.8 %) from the Semi-Intensive Unit. A total of 39 (88.6 %) were identified as Klebsiella pneumoniae, one (2.3 %) was Enterobacter aerogenes, three (6.8 %) were Enterobacter cloacae and one (2.3 %) Klebsiella oxytoca. A total of 35 samples presented positivity, being 20 (57.1 %) from the nursery and 15 (42.9 %) from the ICU. Of these, 24 (68.6 %) were strains of Klebsiella pneumoniae, five (14.2 %) of Enterobacter aerogenes, another 5 of Enterobacter cloacae and one (3 %) of Proteus mirabilis. The phenotypic resistance study revealed a predominance of KPC (Klebsiella pneumoniae carbapenemase) and other phenotypes found were OXA-48/Porin Loss, cAMP and a suspected MBL (metallobetalactamase). Although there is a predominance of KPC, the findings show the need to support the active search for emerging phenotypes that pose a threat to antibiotic therapy. Financial support or fellowship: UNIP Support - Vice-Rectory of Postgraduate and Research

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CFSP353 COMPARATIVE STUDY IN THE DETERMINATION OF THE CONTENT NITRATES OF VEGETABLE “IN NATURA” SOLD IN JUAZEIRO DO NORTE, CEARÁ, BRAZIL JAMYLYS FIDELIS DA SILVA; GUSTAVO DE OLIVEIRA ALENCAR; CAMILA ALENCAR BEZERRA; MARIA DE FÁTIMA ROCHA BARRETO; KARLA DEISY MORAIS BORGES. Faculdade Juazeiro do Norte, Juazeiro do Norte - CE - Brazil. Introduction and objective: Vegetables are essential for the functioning of the body. They are a source of vitamins and minerals and are noted for the low caloric value. Some plants accumulate many nitrates due to the high amount of nitrogen in the soil. It is accumulate mainly in the leaves and the accumulation is due to the amount of light, temperatures and fertilizers used. The excess of toxins may pose a threat to consumers’ health. Adults may develop carcinogenic, mutagenic and teratogenic problems. In children, it causes methemoglobinemia. This study aimed to determine the nitrate content of vegetables consumed in daily life sold in Juazeiro do Norte, Ceará. Materials and methods: This was a quantitative analysis in which the nitrate content of lettuce, peppers and tomatoes were measured, using the spectrophotometer (SP22 - BIOSPECTRO) at 410nm wavelength. It was analyzed based on the reaction of Nitrate contained in the plant with 5% salicylic acid and after 20 minutes 2N sodium hydroxide was added, and at the end the spectrophotometer was read with standard curve realization. Results and conclusion: The levels of nitrate contained in plants were Lettuce I (6,919 ppm), II (3,540 ppm) and III (4,836 ppm), Pepper I (740 ppm), II (5,669 ppm) and III (7,127 ppm ), Tomato I (2,638 ppm), II (10,413 ppm) and III (9788 ppm), with up to 3,500 ppm being allowed. Therefore, the search for healthy food is currently being increasingly used, as exacerbated consumption can pose health risks due to the accumulation of the substance in the body. Financial support or fellowship: FJN

CROP YIELD OF THE LEAVES OF THE SOLANUM PANICULATUM L. (JURUBEBA)

CFSP354

IZABELLA CINTHIA TÔRRES DE VASCONCELOS1; JOICE LUIZA PEREIRA DA SILVA2; JAMICELLY RAYANNA GOMES DA SILVA 2; YASMIM DAYANE LEAL PAIXÃO2; SARA BATISTA VASCONCELOS2; ARQUIMEDES FERNANDES MONTEIRO DE MELO2. ASCES/UNITA, Caruaru - Brazil; 2ASCES - UNITA, Caruaru - PE - Brazil.

Introduction and objective: The Solanum paniculatum L., belongs to the Solanaceae family and it is popularly known as “jurubeba”. The extract from all of the parts of this plant, including its leaves, are mentioned as a molluscicide, with antiinflammatory, hepatoprotective and diuretic activity. Taking into account this information, the aim was to analyze the yield of the Dry Gross Extract from the Jurubeba’s leaves. Materials and methods: The leaves were collected and weighted, totaling 624g, then they were crushed and macerated for 7 days in a hydroalcoholic extractive solution 95% (v/v). After this procedure, the extrusion and the evaporation of the extractive solution were performed until the loss of 95% of its humidity. Then, the extract was put in B.O.D. to obtain the Dry Gross Extract, resulting in 16.78g. The yield of the extract was calculated according to the formula: Yield= (Weight of the dry gross extract/ weight of the fresh leaves) x 100. Results and conclusion: The Yield of The Dry Gross Extract from the leaves was obtained = 2.68%. It was concluded that the calculation of the Yield of plant material is useful to determine a quality pattern of the material studied, using this as reference for future research involving the extract from this part of the plant. Financial support or fellowship: ASCES-UNITA

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CFSP355 CYTOKINE PROFILE AND IMMUNOGLOBULINS G AND M ANTI-TOXOPLASMOSIS IN SERUM OF PREGNANT WOMEN PRISCILA DOMINGOS DE FREITAS; ANA PAULA SIMOES BECKMANN; ALLANA APARECIDA RIBEIRO PRADO; DANNY LAURA GOMES FAGUNDES TRICHES. Universidade Federal de Mato Grosso, Barra do Garças - MT - Brazil. Introduction and objective: Toxoplasmosis is a disease caused by the protozoan Toxoplasma gondii, an intracellular parasite with a worldwide spread, affecting about 15 to 85% of the human population. The objective of this work was to evaluate the cytokine profile (IL-2, IL-6, IL-8, IL-10, IL-12 and TNF-α), as well as to quantify the anti-Toxoplasma gondii antibodies of the IgG and IgM subclasses in serum of pregnant women. Materials and methods: This is a cross-sectional study, carried out with 34 pregnant women attending the public network in the municipality of Barra do Garças-MT. After signing the TCLE, the biological material was collected for cytokine and IgG and IgM antibody titers. Cytometric Bead Array kits (CBA, BD Bioscience, USA) were used by the method of Flow cytometry, and Symbiosis® Anti-Toxo IgG and IgM by the ELISA technique, respectively. Results and conclusion: The predominant age group was 21-30 years old with 58.82%, with the second trimester of gestation prevailing. The results of the cytokines were obtained by FCAP Array v.3.0 software and expressed in pg/ml, the cytokine profile was found to be mixed, common in T. gondii infections. In relation to IgG, 61.11% were considered immune (25.83 UI/mL), and 38.89% were nonimmune (8.17 UI/mL); for IgM all the pregnant women were considered non-reactive. As the pregnant women were not in the acute stage of the disease, no significant difference was observed between cytokine levels and serum antibody concentration. It is known that the follow-up and performance of prenatal examinations, as well as guidance on the risks of this disease in pregnancy, associated with the protective immunological mechanisms can avoid the complications of the congenital form of Toxoplasmosis. Financial support or fellowship: FAPEMAT CFSP356 DETERMINATION OF THE HEMOLYSIS PERCENTAGE OF PINE (ANNONA SQUAMOSA) TOWARDS SHEEP BLOOD CELLS ALYNE LETICIA BEZERRA GOMES RAFAEL1; RAISSA FERREIRA SOARES1; MARIA EDUARDA SILVA AMORIM1; MARIA GABRIELLE DE OLIVEIRA TABOSA1; JAMICELLY RAYANNA GOMES DA SILVA1; RISONILDO PEREIRA CORDEIRO2; JULIA SAMARA FERREIRA SILVA1. ASCES-UNITA, Caruaru - PE - Brazil; 2ASCES-Uni8ta, Caruaru - PE - Brazil.

Introduction and objective: Annona squamosa, commonly known as Pine or Count-Fruit, belongs to Magnoliidae family and has various biological activities, such as analgesic, anti-inflammatory and cytotoxic. The objective of this study was to analyze the erythrocyte osmotic fragility (EOF) of Pine’s twig and leaves. Materials and methods: 50 mg of Pine (twig and leaves) dry crude extract (DCE) was diluted in 0.9% saline solution and the volume was completed to 5 mL with the same diluent. In a control test tube, 50 μL of sheep’s blood was added. In another six test tubes, 25 μL of 1000, 750, 500, 250, 100 and 50 μg/mL of Pine DCE were respectively added with 25 μL of sheep’s blood. The samples were incubated at 30 ºC and then centrifuged for 15 minutes at 3500 RPM. The supernatant was isolated and subjected to spectrophotometric analysis, aiming to quantify absorbance at the wavelength of 546 nm, in Bioplus spectrophotometer. All tests were performed in duplicate. Results and conclusion: At the highest concentration (1000 ug/mL), for both parts of the plant, hemolysis values of 12.80% (twig) and 46.51% (leaves) were obtained. These results confirm that Pine DCE presented, respectively, a moderate and high percentage of lysis of the erythrocytes at the tested parameters and conditions. Financial support or fellowship: ASCES-UNITA

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CFSP357 DETERMINATION OF THE LEVELS OF METHEMOGLOBIN IN PATIENTS INFECTED BY MYCOBACTERIUM TUBERCULOSIS. NORMA LUCIENE LIMA DA SILVA; SILVIA LETÍCIA DE FRANÇA GASPAR; CLARISSE ANDRADE SALES; JUAN GONZALO BARDÁLEZ RIVERA; JOSÉ LUIZ FERNANDES VIEIRA. Ufpa, Belém - PA - Brazil. Introduction and objective: According to the World Health Organization, one in three people on the planet carries the Koch bacillus , a bacterium that causes TB. The Ministry of Health recommends the basic scheme of chemoprophylaxis for a period of six months, composed of the following drugs: Rifampicin (R), Isoniazid (H), Pyrazinamide (Z) and Etambutol (E). Use of isoniazid during treatment against bacillus may cause toxic effects. Objective: to determine the levels of methemoglobin in patients who use isoniazid and who are affected by Mycobacterium tuberculosis Materials and methods: Blood samples were collected from 34 patients diagnosed with TB, attended at the Tuberculosis clinic of Joao de Barros Barreto-UFPA University Hospital and the Guama-SESMA-BELEM Basic Unit, during six months (D0, D1, D2, D3, D4, D5, D6). Blood samples were taken to the Toxicology Laboratory -UFPA, to perform the determinations of methemoglobin by UV- spectrophotometry, according to Camargo et al., 2007 method. Results and conclusion: Analyzed the levels of methemoglobin, were seen: D0(1.59 ± 0.63); D1(4.30 ± 1.43); D2(6.87 ± 1.85); D3(4.81 ± 1.93); D4(3.26 ± 1.76); D5(2.2 ± 1.18), D6(1.33 ± 0.64). With significance D3XD4(p<0,0001 e r=0,80); D4XD5(p <0.0001 and r = 0.80) and D5XD6(p <0.0001 and r = 0.86). These results showed that until D2, methemoglobin values exceeded the reference (2%). In the end of the treatment, these values decrease to normal, corroborating with the p value. These results suggest high levels of methemoglobin is associated with joint administration of H, R and Z, what leads to an increased in hepatic function and decreased of renal function. In the end of treatment and Z withdrawal, methemoglobin values back to normal, which relates a farmacocinetic interaction and blood reactive species production. Financial support or fellowship: CNPq

EFFECTS OF METHYLPHENIDATE ON DENTAL GERM IN PREGNANT MICE

CFSP358

CAMILA SALVADOR SESTARIO; KAROL SARTORI LIMA; SOLANGE DE PAULA RAMOS; MARIA JOSÉ SPARÇA SALLES. Universidade Estadual de Londrina, Londrina - PR - Brazil. Introduction and objective: Methylphenidate hydrochloride (MFD) is a Central Nervous System stimulator structurally and pharmacologically similar to amphetamine. It is a psychostimulant used to treat Attention Deficit Hyperactivity Disorder, however, the administration of this drug during pregnancy is not safe in relation to the risks to the embryo. Thus, the objective of this work was to evaluate the effects of MFD exposure on the embryonic development of dental germ in mice. Materials and methods: Thirty-two pregnant adult Swiss mice (Mus musculus) weighing 35g each were used. For the prenatal study, 2 groups were separated. The treated group received 5 mg/kg of Ritalin (Novartis) and control group received sterile saline via gavage. For the histological evaluation of odontogenesis, the first fetus of the right uterine horn of each female was separated and had its head removed. The head were fixed and processed for histological procedure. The cut plane chosen was parallel to the longitudinal axis of the molar, demonstrating the region of the enamel organ and the dental papilla. For analysis of the association between the dependent variables and the groups, chi-square and exact tests were used. The parameters analyzed were: alteration of tooth germ form, presence of mitotic figures, polarization of ameloblasts core, palisade ameloblasts, disruption of the ameloblasts layer, delay in odontoblasts secretion, presence of the components of the tooth germ (enamel organ papilla, follicle), presence of Hertwig’s epithelial root sheet’s sheath. Results and conclusion: The parameter that presented statistical relevance was the occurrence of changes in 43.8% in the form of the enamel organ (p = 0.035) of the treated group, while there was no change in the control group. It is concluded that exposure to methylphenidate during gestation alters the development of the dental germ of the offspring of mice. The administration of drugs during the first trimester of gestation may interfere with the development, differentiation and growth of dental germs. The dentist is responsible for advising pregnant women about medications that may affect fetal development. Financial support or fellowship: CAPES

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CFSP359 EVALUATION OF CYTOTOXICITY AND INHIBITION CAPACITY OF THE PRODUCTION OF INFLAMMATORY FACTORS BY HYDROALCOHOLIC EXTRACT AND ETHYL ACETATE AND BUTANOL FRACTIONS OF VITEX POLYGAMA LEAVES ISABELA SOUZA DOS SANTOS; RENATA DE JESUS MELLO; MARLON HEGGDORNE DE ARAUJO; CRISTIANE CATELA MARTINS DE JESUS; MICHELLE MUZITANO FRAZÃO; SHAFT CORRÊA PINTO; JULIANA MONTANI RAIMUNDO; PAULA LIMA DO CARMO. URFJ - Universidade Federal do Rio de Janeiro, Macaé - RJ - Brazil. Introduction and objective: We previously demonstrated the antinociceptive and anti-inflammatory effects in vivo of the hydroalcoholic extract (HE) and ethyl acetate (EAF) and butanol (BF) fractions of Vitex polygama leaves. The aim of this study was to evaluate the cytotoxicity and inhibition of the production of nitric oxide (NO) and tumor necrosis factor alpha (TNF-α) in vitro. Materials and methods: The culture supernatant of murine macrophages (RAW 264.7) cells was replaced with DMEM-F12 supplemented with 2% FBS in the presence or absence of 1μg/ml LPS (Escherichia coli). Cells were treated with HE, EAF and BF (0.8-500μg/ml). After 24 h, the supernatant was collected to assess the ability to inhibit NO and TNF-α production. The cytotoxicity test was performed by MTT method. Results and conclusion: HE and EAF showed a cytotoxic effect only at 500μg/mL (31.87±6.51% and 88.46±0.05%, respectively). BF did not show significant cytotoxicity at any of the concentrations evaluated. HE, EAF and BF (100μg/mL) inhibited NO production at 73.10±8.18%, 91.05±6.51% and 34.14±14.07% Financial support or fellowship: FAPERJ. CFSP360 EVALUATION OF THE ANTIBACTERIAL PROFILE OF N-ACYLHYDRAZONE DERIVATIVES CONDENSED TO NITROGEN HETEROCYCLIC RINGS MIGUEL ANGELO SANTOS PINHEIRO SEGUNDO 1; WILLIAN CHARLES DA SILVA MOURA 2; DENISE NASCIMENTO PEREIRA2; JAMIRE MURIEL DA SILVA2; ZILKA NANES LIMA2; RICARDO OLIMPIO DE MOURA2. Ufpe, Recife - PE - Brazil; 2Uepb, Campina Grande - PB - Brazil.

Introduction and objective: Acylhydrazone derivatives are an important class of synthetic chemical compounds, since their various biological activities have been described in the literature. Thus, the objective of this work was to evaluate the antibacterial profile of N-acylhidrazone derivatives condensed to nitrogen heterocyclics (pyridine, quinoline, indol and acridine). Materials and methods: The compounds were tested in vitro against the Gram-positive (Staphylococcus Aureus ATCC 25923) and Gram-negative (Escherichia coli ATCC 25922) strain, from 1024 μg/mL to 1 μg/mL. The tests were performed in triplicate. The culture medium used in the tests was MH broth. The control drug used was gentamicin and some wells were reserved for tests of sterility of the medium and control of bacterial growth. Results and conclusion: When analyzing the structures and correlating them with the microbiological result, it was verified that the compounds containing the indole and quinoline core showed the same activity for the two bacteria tested (MIC of 512 μg/ mL), even one containing a heteroaromatic ring with 6 and another with 5 members. However, the pyridine derivative showed decreased activity to Gram-positive strain (MIC of 1024 μg/mL). Nevertheless, the acridine derivative, which is more deficient in electrons, was the most active in both strains (256 μg/mL). Chosen as the best compound of the series tested. Financial support or fellowship: CAPES, UFPE.

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CFSP361 EVALUATION OF THE EFFECT OF CHRONIC SELENIUM SUPPLEMENTATION ON THE GROWTH OF ERLICH TRANSPLANTABLE TUMOR AND ITS TOXICITY IN BALB-C MICE. JOSE FRANCISCO RAMOS DOS SANTOS 1; JULIANA MORA VERIDIANO 1; GIULIANA PETRI 1; ADRIANA HELENA ALMEIDA1; BRUNO MACHADO BERTASSOLI1; OLGA MARIA DE TOLEDO CORREA1; FERNANDO LUIS AFFONSO FONSECA1; TÂNIA AGUIAR PASSETI2. Fmabc - Faculdade de Medicina do Abc, Santo André - SP - Brazil; 2Fmabc, Santo André - SP - Brazil.

Introduction and objective: This work proposes to evaluate the effect of selenium supplementation in the growth of Erlich Transplantable Tumor (ETT) in Balb-c mice and its toxic effects. Materials and methods: 60 male Balb-c mice were inoculated with 2x106 cells of ETT in the dorsal region. After 7 days, the mice were divided into 2 groups; group 1: the animals received oral daily supplementation of selenium quelate in three doses (1.43; 2.86 and 4.29µg/Kg in 0.2 ml saline solution), group 2: received oral daily saline solution (0.2ml). Both groups were treated for 7, 15 and 30 days. After the end of treatments, the animals were euthanized with anesthetic overdoses and blood samples were collected to measure ALT and AST enzymes. Several organs were collected for histological analysis. The tumors were surgically removed and the weight was measured in an analytical balance. Results and conclusion: After 7 days treatment a 50-60% decrease was observed in tumor weight when compared to control group Financial support or fellowship: Faculdade de Medicina do ABC, Santo André, São Paulo – Brazil. CFSP362 GENETIC VARIANTS IN LEPTIN-MALANOCORTIN PATHWAY PROTEINS AS BIOMARKERS FOR EARLY DIAGNOSIS OF OBESITY AND METABOLIC SYNDROME IN CHILEAN POPULATION VICTOR MANRIQUEZ1; JORGE AVILES1; LUIS SALAZAR2; NICOLAS SAAVEDRA2; FERNANDO LANAS3; PAMELA SERON3; MARIO HIRATA4; ROSARIO DOMINGUEZ CRESPO HIRATA4; ALVARO CERDA5. Center of Excellence in Translational Medicine, Universidad de La Frontera, Temuco - Chile; 2Center of Molecular Biology and Pharmacogenetics, Universidad de La Frontera, Temuco - Chile; 3Department of Internal Medicine, Universidad de La Frontera, Temuco - Chile; 4School of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo - SP - Brazil; 5Center of Excellence in Translational Medicine, Universidad de La Frontera - Chile. 1

Introduction and objective: Polymorphisms in genes encoding proteins of the leptin-melanocortin pathway have been associated with obesity. The involvement of these polymorphisms with changes in body mass index (BMI) and anthropometric measures could also imply a contribution to the risk of metabolic syndrome (MetS) and metabolic alterations. We evaluated the relationship of leptin-melanocortin system polymorphisms with obesity, MetS and other metabolic alterations in Southern Chilean individuals Materials and methods: Two-hundred individuals were grouped as normoweight (BMI 18.0-24.9 Kg/m2, n=68), overweight (BMI 25.0-29.9 Kg/m2, n=67) and obese (BMI ≥30 Kg/m2, n=65) or according to MetS status (MetS, n=100; non-MetS, n=100). Anthropometric measures (BMI, abdominal circumference, waist-to-hip ratio -WHR) and biochemical parameters (glycaemia and lipid profile) were evaluated. Polymorphisms LEP c.-2548A>G, LEPR c.668A>G, MC3R c.17C>A and c.241G>A and MC4R g.60183864T>C were evaluated by real-time PCR using allelic discrimination assays Results and conclusion: As expected, obese and MetS individuals presented a more atherogenic profile according to anthropometric measures and to glycaemia and lipid profile parameters, as compared with normoweight and non-MetS subjects (pA were also associated to altered lipid profile Financial support or fellowship: FONDECYT #11150445; DIUFRO #DI15-0017; CNPp #462778/2014-2

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CFSP363 GLOBAL EXPRESSION OF miRNAs IDENTIFIES miR-155 AS A BIOMARKER AND POTENTIAL PHARMACOLOGICAL TARGET FOR CARDIOMETABOLIC RISK AMONG OBESE PATIENTS ALVARO CERDA1; ADONAI AMARAL2; RAQUEL DE OLIVEIRA2; AECIO BRAGA2; ELIZABETH GRACIANO2; CRISTINA FAJARDO2; EGIDIO LIMA DOREA3; THIAGO HIRATA2; MARIO HIRATA2; ROSARIO DOMINGUEZ CRESPO HIRATA2. Center of Excellence in Translational Medicine, Universidad de La Frontera, Temuco - Chile; 2School of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo - SP - Brazil; 3University Hospital, University of Sao Paulo, Sao Paulo - SP - Brazil. 1

Introduction and objective: Obesity has become one of the main health problems worldwide. The high number of obese individuals highlight the need to develop new biomarkers to identify obese patients at a high cardiometabolic risk, such as those with high indices of insulin resistance (IR). This work aimed to identify microRNAs (miRNAs) as molecular biomarkers by screening global miRNA expression using next generation sequencing (NGS). Materials and methods: Firstly, 12 obese patients (6 IR and 6 non-IR according to HOMA-IR, matched for sex, age and BMI) were evaluated for global miRNA expression using a NGS platform (MiSeq, Illumina). Then, 80 obese individuals were evaluated to analyze the expression of selected miRNAs. RNA was extracted from leucocytes and miRNA libraries were prepared using the TruSeq small RNA sample preparation kit (Illumina, USA). Sequences and differential expression were evaluated using the MiSeq Reporter and CLC Bio Workbench software. Selection of miRNAs, interactions with target miRNAs and their metabolic pathways were evaluated using the Ingenuity Pathway Analysis-IPA software. The expression of selected miRNAs was evaluated by qPCR Results and conclusion: Ten miRNAs were differentially expressed between IR and non-IR obese patients (let-7i, miR-181a, let-7a, miR-155, let-7f, miR-28, miR-363, let-7g, miR-1839, miR-30d; all of them downregulated in IR). miR-181, miR-155 and let-7a are involved in the signaling pathway of insulin receptor and therefore, these miRNAs were selected for further validation. Individuals with higher values of HOMA-IR (4th quartile; HOMA-IR >7.6) had lower expression of miR-155 Financial support or fellowship: FONDECYT #11150445; CNPp #457334/2013-4 CFSP364 IN SILICO PREDICTION STUDIES OF BIOLOGICAL ACTIVITY AND TOXICITY OF FLAVONOIDS ISOLATED FROM UNCARIA GUIANENSIS (AUBL.) GMEL. LETICIA HIROMI OHASHI; ANALU DE MARIA DOS SANTOS DAMASCENO; VALDICLEY VIEIRA VALE; MARIA FANI DOLABELA. Universidade Federal do Pará, Belém - PA - Brazil. Introduction and objective: Uncaria guianensis (or cat’s claw) is an Amazonian herbal medicine that is widely used to treat various diseases, as well as being widely used to treat inflammatory disorders. Several secondary metabolites have already been isolated from this plant, among them alkaloids and flavonoids. Therefore, the objective of this work is to study biological activity and toxicity of flavonoids isolated from uncaria guianensis through in silico studies. Materials and methods: First, a literature search was conducted in the SCIELO, GOOGLE SCHOLAR, LILACS databases, with articles published on flavonoids isolated from Uncaria guianensis. Through the studies in these databases it was possible to identify three flavonoids isolated from uncaria guianensis, Kaemferol, Dihydrokaemferol and Kaempferitrin. These substances previously elucidated in the literature were subjected to in silico test by the PreADMET and Pass online software. Results and conclusion: Among the main biological activities of the secondary metabolites studied is membrane integrity agonist. This result is repeated in all studied flavonoids. Some results were similar for the substances tested in the study, because the program does not take into account isomerism, taking into account only the central nucleus of the molecule. Besides this result, the Kaemferol flavonoid was suggested to be a chlordecone reductase inhibitor, Kaempferitrin was suggested to be hemostatic and the flavonoid dihydrohydroememol was suggested to be an APOA1 expression enhancer. In in silico toxicological studies, a possible mutagenic action was demonstrated by the Ames test of the substances Kaemferol and Dihydrokaempferol, while the substance Kaempferitrin was shown to be non-mutagenic. In the prediction of Daphnia toxicity, only kaemferol was shown to be toxic. In the prediction of Daphnia toxicity and medaka fish, only kaemferol was shown to be highly toxic. While dihydrokaempherol and Kaempferitrin were considered to be very toxic and moderately toxic in the medaka fish test, respectively, and were not considered to be toxic in Daphnia. The results of the in silico studies demonstrated a potential toxicity of these substances. Financial support or fellowship: FAPESPA

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INCIDENCE OF URINARY INFECTION IN ADOLESCENTS AT A PUBLIC HOSPITAL

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CFSP365

REINALDO SOUZA MIRANDA; ADRIANA APARECIDA FELTRIN CORREA; MARIANNA VAZ RODRIGUES; FÁTIMA HADDAD BARRACH. UNIP, Bauru - SP - Brazil. Introduction and objective: Urinary Tract Infection (UTI) is one of the most common pathologies in humans, from the neonatal to the adult stage and can be caused by microbial agents in general. The disease affects both sexes, although the prevalence is higher in females due to the proximity of the vagina to the anal region. The objective of this study was to conduct a retrospective cross-sectional study to determine the incidence of urinary tract infection in adolescents attended at a public hospital in the city of Bauru/SP. Materials and methods: A total of 5047 electronic records of adolescents aged 10 to 19 years with suspicion of urinary tract infection (UTI) attended at the Hospital Estadual de Bauru as both outpatients and admissions, from January 1, 2010 to January 31, 2017 were analyzed. Results and conclusion: Of the medical records analyzed, 11.04% (561/5047) presented positive results for UTI, 71.84% (403/577) were female and 28.16% (158/577) male. Statistical analysis showed that the odds ratio, which is 0.52, should interpret that women are 52% more likely to have infections than men. Regarding the attendance regimen in which the UTIs were identified, there was no statistical difference (p = 0.4797). Among the bacteria analyzed, Escherichia coli appeared in 56.14% (315/561) of cases followed by Klebsiella pneumoniae with 8.02% (45/561) and Proteus mirabilis with 6.95% (39/561). The Gram negative bacteria represents 89.30% (501/561) of the positive results, Gram positive 9.80% (55/561) and 0.90% (5/561) for fungi. It was observed that E. coli was the uropathogen that prevailed in UTI in adolescents followed by K. pneumoniae and P. mirabilis, both of which presented resistance to ampicillin 10ug. Financial support or fellowship: UNIP CFSP366 INDUCTION OF ACUTE KIDNEY INJURY IN LABORATORY RATS BY CROTALUS DURISSUS TERRIFICUS AND BOTHROPS JARARACA VENOMS FOR THE DEVELOPMENT OF A HEMOPERFUSION CARTRIDGE EDSON HIDEAKI YOSHIDA; MURILO MELO JUSTE DINI; VALQUIRIA MIWA HANAI; DENISE GROTTO; YOKO OSHIMA FRANCO. Universidade de Sorocaba, Sorocaba - SP - Brazil. Introduction and objective: A serious complication to the intoxication caused by venomous serpents (genus Bothrops and Crotalus) is Acute Kidney Injury (AKI). The prevention of AKI is through early administration of the specific antivenom, treatment for hypotension and shock, maintenance of an adequate hydration status and hemodialysis. Objective: To induce AKI in animal model using Crotalus durissus terrificus and Bothrops jararaca venom. Materials and methods: The induction of AKI two hours after the injection of venom with doses of DL50, in male Wistar (CEUA nº 031/2014) rats. The rats were divided into three groups: a control group not exposed to the venom, a group exposed to Crotalus durissus terrificus venom and a group exposed to Bothrops jararaca venom. The groups were evaluated by biochemical parameters (urea and creatinine), by hematological parameters (leukocytes, platelets and erythrocytes) and by kidney and blood oxidative stress parameters (reduced glutathione, glutathione peroxidase, catalase and substances reactive to thiobarbituric acid. Results and conclusion: In the results, two hours after the induction of AKI, the occurrence of statistic significant alteration (p˂0.05) of the biochemical parameters, of the hematological parameters and of the kidney oxidative stress parameters were demonstrated. It was possible to confirm the induction of AKI in rats, two hours after the ophidian venom injection. This confirmation allows the development of a cartridge for a hemoperfusion system as an alternative treatment for ophidian incidents. Financial support or fellowship: FAPESP; CAPES.

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CFSP367 KIDNEY PROFILE ANALYSIS OF ATHLETES WITH DIETARY SUPPLEMENTS FROM UREA AND CREATININE SERUM BLOOD QUANTIFICATION AND FROM AN ESTIMATED CREATININE CLEARANCE ANDRÉ LUIZ GONZAGA DE MORAES; FÁTIMA HADDAD BARRACH; MARCO ANTONIO VIEIRA DA SILVA; TERESA CRISTINA DE BARROS. Universidade Paulista (UNIP/Bauru), Bauru - SP - Brazil. Introduction and objective: Dietary supplementation, be it with protein or free amino acids, increases the synthesis of urea by the liver. This increase can easily lead to a renal deterioration when supplementation is done in abusive way. Kidney function decrease can be detected through the urea and creatinine quantification in the blood serum. Creatinine clearance is an example of another test used at to diagnose kidney disease. Therefore, the objective of this study was to verify whether consumption of dietary supplements is affecting the kidney function of sportsmen. Materials and methods: Twenty-nine individuals were selected for the experimental group, those who are adepts of dietary supplementation, and 8 individuals for the control group, exempt from dietary supplementation. Then, the urea and creatinine quantification from participants’ blood of was conducted. For the urea quantification, the Enzymatic Colorimetric method was applied, while in the quantification of creatinine the method used was the Jaffé’s reaction, creatinine clearance being estimated from the Cockcroft-Gault equation. Results and conclusion: Unequal levels of urea and creatinine were observed between the experimental and control groups. Among those individuals who use dietary supplementation 41.38% had alterations in the urea levels, 46.43% had alterations in the creatinine levels, 39.28% had reductions in the glomerular filtration rate and 17.20% had alterations in the 3 criteria at the same time. However, some of the individuals showed minimal alterations from the biochemical point of view. Nevertheless, changes and variations between results, although small, were detected. Thus, the data support the possibility of kidney damage if there is abuse of supplementation without professional supervision. Financial support or fellowship: CNPq; UNIP

OVERVIEW OF HUMAN INTOXICATION BY MEDICINES IN BRAZIL: 2008-2013

CFSP368

CAROLINY RIBEIRO DE MELO NUNES; JACKELYNE ROBERTA SCHERF; GUSTAVO DE OLIVEIRA ALENCAR; CAMILA ALENCAR BEZERRA; MARIA DE FÁTIMA ROCHA BARRETO; EMANUELA MACHADO SILVA SARAIVA. Faculdade de Juazeiro do Norte, Juazeiro do Norte - CE - Brazil. Introduction and objective: Pharmaceutical drugs have been the main toxic agent in cases of human intoxication, since 1994. Among the countries that are drug consumers, Brazil is fifth in the world and first in the Latin America. The National Toxicological Information System (SINITOX) is responsible for publishing annual statistics on cases of intoxication recorded by the Toxicological Information and Assistance Centers (CEATOX). This study aimed to evaluate the data on human intoxication caused by pharmaceutical drugs in Brazil. Materials and methods: This is a quantitative and exploratory approach to data obtained from SINITOX from 2008 to 2013. Results and conclusion: It was verified that Brazil obtained 150,361 registered cases of human intoxication by pharmaceutical drugs. Over the years, there has been a gradual increase in these intoxications, mainly between the years of 2008 and 2013. 2011 was the year which presented the most notifications, corresponding to 20.12% of registered cases. The Southeast region of Brazil presented more cases of intoxication than others, about 56%. Therefore, the promotion of rational use and access to medicines is necessary, given the number of registered cases. It is important that the facts presented may be even greater since notifications to SINITOX are voluntary. Financial support or fellowship: SINITOX

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CFSP369 POLYMORPHISMS IN MTOR AND CALCINEURIN PATHWAY-RELATED GENES INFLUENCE CLINICAL RESPONSE AT ONE-YEAR POST-TRANSPLANT IN KIDNEY TRANSPLANT RECIPIENTS ANTONY BRAYAN CAMPOS SALAZAR1; FABIANA DALLA VECCHIA GENVIGIR2; CLAUDIA ROSSO FELIPE3; HELIO TEDESCO SILVA JUNIOR3; JOSÉ OSMAR MEDINA PESTANA3; MARIO HIRATA1; ALVARO CERDA4; ROSARIO DOMINGUEZ CRESPO HIRATA1. School of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo - SP - Brazil; 2School of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo - Brazil; 3Nephrology Division, Hospital do Rim, Universidade Federal de Sao Paulo, Sao Paulo - SP - Brazil; 4Center of Excellence in Translational Medicine, Universidad de La Frontera, Temuco - Chile. 1

Introduction and objective: Polymorphisms in pharmacodynamic-related genes of immunosuppressive drugs may be important sources of individual variability in efficacy and safety during treatment. Objective: To investigate the influence of polymorphisms in mTOR and calcineurin pathway-related genes on the clinical outcome in kidney transplant recipients in the first-year post-transplant. Materials and methods: Two-hundred sixty-nine kidney transplant recipients were recruited at the Hospital do Rim e Hipertensão, Brazil, and were randomized in three groups receiving, either alone or combined, tacrolimus, everolimus and mycophenolate sodium. Clinical and laboratory data were recorded. Genomic DNA was extracted from blood samples to analyze gene polymorphisms (MTOR rs1057079, rs1135172 and rs1064261; PPP3CA rs3730251; FKBP1A rs6033557; FBKP2 rs2159370; FOXP3 rs2232365 and rs3761548) by real-time PCR. Results and conclusion: After 1-year post-transplant, serum creatinine levels of FOXP3 rs3761548 CC individuals were higher than CA+AA (p=0.018). Gastrointestinal and metabolic adverse events were present in 52.4% and 41.3% of the patients, respectively. Carriers of the FKBP1A rs6033557 TC+CC and FOXP3 rs2232365 GA+AA genotypes showed a higher risk of presenting constipation and epigastric pain events, respectively (OR=2.52, 95%CI=1.13–5.61, p=0.047; OR=2.15, 95%CI=1.01– 4.56, p=0.038), while FKBP2 rs2159370 GG and FOXP3 rs2232365 GG individuals were associated with higher incidence of dyslipidemia (OR=1.92, 95%CI=1.00–3.69, p=0.049; OR=2.06, 95%CI=1.08–3.92, p=0.028, respectively). In conclusion, FOXP3 rs3761548 CC genotype is associated with poor renal function at 1-year post-transplantation, and carriers of the genetic variants, FKBP1A rs6033557, FKBP2 rs2159370 and FOXP3 rs2232365, present more susceptible to adverse events. Financial support or fellowship: CAPES; CNPq; FAPESP. CFSP370 PREDICTION AND SYNTHESIS OF A CONFORMATIONAL EPITOPE OF TS3, A TITYUS SERRULATUS ALPHATOXIN, USING BIOINFORMATIC TOOLS ELLEN DE PIERI1; MÍRIAN IVENS FAGUNDES1; MÁRCIA PEREIRA1; RAHISA SCUSSEL1; BETHINA STEINER1; LUIZA MACARINI BOSA1; CARLOS CHÁVEZ-OLORTEGUI2; RICARDO ANDREZ MACHADO DE ÁVILA1. Universidade do Extremo Sul Catarinense, Criciúma - SC - Brazil; 2Universidade Federal de Minas Gerais, Belo Horizonte MG - Brazil. 1

Introduction and objective: Tityus serrulatus, popularly known as yellow scorpion, is the species which has the most toxic venom in Brazil. They are responsible for the majority of accidents with venomous animals in the country. The Ministry of Health recommend anti-venom (anti-scorpion serum) treatment, produced by equine immunization, in case of envenomation. However, this methodology generates some bioethical and economic issues, related to venom toxicity and the costs of maintaining the animal used. Therefore, the present study aims to search for an alternative, synthetic and non-toxic, antigen for anti-venom production. This antigen is based on the prediction of a conformational epitope of Ts3, an alpha-toxin from the scorpion venom Tityus serrulatus, capable of causing membrane depolarization by acting on site 3 of voltage gated sodium channel (VGSC). Materials and methods: Bioinformatics tools were used to predict a conformational epitope of Ts3. The conformational epitope was synthetized by Fmoc-synthesis method and used as an immunogen for the immunization of Swiss mice. ELISA assay was used to evaluate the antibodies produced. Results and conclusion: The protein model structure was obtained, the prediction of the conformational epitope was performed and the peptide was synthesized. Peptide validation was conducted using ELISA assay. The results showed that the peptide was capable of inducing antibody production in mice. In conclusion, the synthetic peptide has shown to be an alternative to replace the use of crude scorpion venom in equine immunization for anti-venom production. Financial support or fellowship: UNESC; FAPESP; CAPES; CNPq

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CFSP371 PREVALENCE OF COAGULASE NEGATIVE STAPHYLOCOCCI (CNS) IN INTENSIVE CARE UNIT (ICU) OF BAURU STATE HOSPITAL – SP SADI NASCIMENTO ROCHA JUNIOR; ADRIANA APARECIDA FELTRIN CORREA; FÁTIMA HADDAD BARRACH. UNIP, Bauru - SP - Brazil. Introduction and objective: Coagulase Negative Staphylococci (CNS) are Gram-positive bacteria and are part of the normal skin microbiota , in the last two decades the number of infections by CNS has increased significantly, this increase is due to the use of implanted medical devices such as catheters and prostheses, and the resistance of these microorganisms to antimicrobials. The aim of this study was to verify the prevalence of Coagulase Negative Staphylococci (CNS) in samples of organic liquids (urine, blood, liquor and other body fluids) in ICUs of the State Hospital of Bauru - SP, as well as to identify the resistance profile of these microorganisms against the tested antibiotics. Materials and methods: A total of 5,432 microbiological cultures from the Microbiology Laboratory of the State Hospital of Bauru of adult patients aged 18 years and older hospitalized in ICUs from January 2010 to December 2016 were analyzed. Results and conclusion: Of the analyzed results, 4% (212/5432) were positive for CNS in all the ICUs analyzed. There was a year-on-year increase in the adult ICU, and in 2010 there were 6 patients with CNS and in 2016 40 patients. The mean age of the patients hospitalized in the ICUs with positive results for CNS was 60.6, and men were the most affected with 61% (128/212). Among the cultures positive for CNS, 56.3% (119/212) were in blood samples, 18.4% (39/212) in catheter tip samples and 9.9% (21/212) in samples of tracheal secretion. The antibiogram showed a greater resistance profile of the CNS to bezilpenicillin, ciprofloxacin, climdamycin, erythromycin and oxacillin. Thus, it was concluded that the increase of this microorganism in recent years may be due to the increase of invasive procedures, considering that the CNS are part of the normal microbiota of the skin and mucosa. Financial support or fellowship: UNIP CFSP372 PREVALENCE OF INFECTIONS BY MULTIRESISTANT BACTERIA IN PEDIATRIC PATIENTS AT A UNIVERSITY HOSPITAL RAFAELE MARIA TIROLLA; JENNIFER RODRIGUES DE LIMA; THAYLA NADRIELLY APARECIDA NICOLINO INSUE; SYLVIA CLAY GOMES MAGALHAES; FELIPE CREPALDI DUARTE; GILSELENA KERBAUY; MARCIA REGINA ECHES PERUGINI. Universidade Estadual de Londrina, Londrina - PR - Brazil. Introduction and objective: Hospital infection (IH) is an event that affects all health institutions, and IH is increasingly involved with multiresistant bacteria (MRB) representing one of the main causes of hospital mortality. Occurrence in pediatric patients is common, therefore, the objective of this study was to evaluate the prevalence of MRB in pediatric patients from the Hospital Universitário Regional do Norte do Paraná (HURNP). Materials and methods: Cross-sectional study of the years 2015 and 2016. All the patients hospitalized in the HURNP pediatrics units were evaluated. Crops and subsequent antibiograms used swabs as analytical material. The study was approved by the ethics committee. Results and conclusion: The incidence of MRB infection was x ≃ 19.68 +/- 9.71. There was a significant seasonal variation in the incidence of infections and the most frequent MRB were Klebsiella spp., Serratia spp. and Enterobacter spp. Infection is associated with several factors, such as factors related to the health unit, factors of the patient, but especially to the contact of health professionals and the caregiver with the child. In this way, knowledge of the prevalence profile and the MRB in a hospital is essential to guide the conduct and appropriate treatment of the patient. Financial support or fellowship: Fundação Araucária

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CFSP373 PREVALENCE OF MULTIRESISTANT BACTERIAL INFECTION IN PATIENTS HOSPITALIZED IN NEONATAL UNITS AT A UNIVERSITY HOSPITAL IN LONDRINA JENNIFER RODRIGUES DE LIMA; RAFAELE MARIA TIROLLA; SYLVIA CLAY GOMES MAGALHAES; CYNTHIA MITIE NAKAWAGA; THAYLA NADRIELLY APARECIDA NICOLINO INSUE; FELIPE CREPALDI DUARTE; GILSELENA KERBAUY; MARCIA REGINA ECHES PERUGINI. Universidade Estadual de Londrina, Londrina - PR - Brazil. Introduction and objective: Hospital infections (IH) constitute a relevant cause of morbidity and mortality, presenting a global public health problem and newborns are some of the most affected. The incidence of HI in neonatology is influenced by several variables, such as: infrastructure, treatment unit resources, characteristics of the newborn, established prophylactic measures, available diagnoses and seasonal effects. The aim of this study was to evaluate the prevalence of all multiresistant bacteria (MRB) that affect these patients in HUNP. Materials and methods: Cross-sectional study of the years 2015 and 2016. All the patients hospitalized in the HUNP neonatology units were evaluated. Crops and subsequent antibiograms used swabs as analytical material. The entire study was approved by the ethics committee. Results and conclusion: The incidence of MRB infection was x ≃ 12.2 +/- 9.89. The most frequent MO were Klebsiella spp., Serratia spp. and Enterobacter spp. Transmission is mainly associated with the contact of the health professional and the caregiver with the newborn. It is concluded that there is a high seasonal variation and that knowledge and management of MRB is extremely important to adapt the patient’s therapy and control HI. Financial support or fellowship: Fundação Araucária

PROFILE OF SUICIDES IN MUNICIPALITY OF LEGAL AMAZON

CFSP374

ERICK DE SOUSA SILVA; JAIR MARQUES JUNIOR; ELIANE APARECIDA SUCHARA. UFMT, Barra do Garcas - MT - Brazil. Introduction and objective: Suicide is a worldwide public health problem and is among the leading causes of death in the world. Thus, the objective of this study was to analyze the occurrence and characterize the cases of suicide registered in a municipality of the Legal Amazon between 1999 and 2016. Materials and methods: A descriptive and cross-sectional epidemiological study was carried out. All cases registered as suicides at the Institute of Forensic Medicine (IFM) in Barra do Garças, Mato Grosso, were included. Results and conclusion: Suicide rates per year ranged from 8.6 (2014) to 20.3 (2003). It was more frequent in the male gender (76.6%) in relation to the female gender (23.3%). There was a predominance of adults (77.3%), followed by elderly (13.8%) and adolescents (8.7). Among adults, the predominant range was 20 to 34 years (58.4%), followed by the age range of 35 to 49 years (26.4%) and lastly 50 to 64 years (15%). The most commonly used cause was asphyxia (49.6%), followed by exogenous intoxications (27.0%), where the main toxic agents used were pesticides (60.9%). In relation to the month of occurrence, April presented the highest number of suicides (13.1%) and the lowest was in February (5.1%). As for the day of the week, Sunday had the highest incidence (17.5%), and Thursday the lowest (11.6%). In view of the above, it is concluded that the male gender and the adult age group were more likely to commit self-harm. Thus, projects related to Public Health and prevention strategies are necessary, thinking also of more effective actions aimed at the profile of the victims.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

ROLE OF ACUTE ETHANOL CONSUMPTION IN THE PATHOGENESIS OF SEPSIS

203

CFSP375

LUANA PECCININI MACHADO; BIANCA PISCINATO PIEDADE ROSA; ALAINA FIORAVANTE; CAIO HENRIQUE BONALDO DE OLIVEIRA; ANDRESSA DE FREITAS. Universidade Estadual de Londrina, Londrina - PR - Brazil. Introduction and objective: Sepsis has a wide and significant mortality rate in the world population. Neutrophils are the first line of innate immune system against infectious diseases. There is evidence in the literature that acute ethanol consumption suppresses innate immunity, contributing to an unfavorable clinical outcome of sepsis. Objectives: investigate the role of acute ethanol consumption in neutrophil migration to the infection focus and in the outcome of polymicrobial sepsis induced by cecal ligation and puncture (CLP). Materials and methods: Swiss mice (25g) were subjected to sepsis through the CLP. The animals were randomly divided into four groups: Control (n=4); Ethanol (n=4); Sepsis (n=5); Ethanol + sepsis (n=5). The total cell counts present in the peritoneal cavity were performed in the Neubauer chamber and differential cells counts were conducted on stained slides. The survival was evaluated for seven days, and clinical signs of alertness, mobility, piloerection, diarrhea, and encrusted eyes/dirty nose and tail were determined within the first 18 hours after sepsis induction. Results and conclusion: Our results demonstrate that the animals submitted to sepsis showed a significant recruitment of neutrophils to the infection focus when compared to the control group or the ethanol group. However, acute ethanol exposure significantly reduces the neutrophil migration to peritoneal cavity of mice subjected to sepsis. The survival of the control group and the group that received only ethanol was 100% at the end of the evaluation period. At 18 hours after CLP, 100% of mice from sepsis group were alive compared with 40% of animals acutely exposed to ethanol and subjected to sepsis. This survival rate was reduced, respectively, to 60% and 20%, 24 hours after surgery, and this ratio remained until the end of the analysis. It is also worth pointing out that the mice exposed to ethanol and subjected to sepsis presented more intense clinical manifestations than mice only submitted to CLP. Moreover, the control group and the ethanol group did not present clinical manifestations of sepsis. Thus, the results suggest that acute ethanol intake reduces the neutrophil migration to the inflammatory site and consequently contributes to the mortality observed in this process. Financial support or fellowship: Araucaria Foundation provides a social inclusion scholarship to Alaina Fioravante CFSP376 SUSCEPTIBILITY PROFILE OF KLEBSIELLA PNEUMONIAE ISOLATES FROM URINE CULTURES IN BASIC HEALTH UNIT AND EMERGENCY ROOM IN A MUNICIPALITY OF SOUTHERN BRAZIL EVELYN POLIANA CANDIDO 1; LUIS FELIPE PERUGINI 1; ROSEANE GALDIOLI NAVA 1; JOÃO GABRIEL MATERIAL SONCINI1; LETICIA CARASKI1; IZADORA ROZA MIRANDA1; EDUARDO CAVALCANTE RABELO MENDES1; GIOVANNA TAVARES CALLADO1; WANDER ROGERIO PAVANELLI1; RENATA KATSUKO TAKAYAMA KOBAYASHI1; JULIANA BUCK DIAS1; ZULEICA NAOMI TANO1; ELIANA CAROLINA VESPERO2. Universidade Estadual de Londrina, Londrina - PR - Brazil; 2Universidade Estadual de Londrina, Brasil - PR - Brazil.

Introduction and objective: Indiscriminate use of antibiotics in the community has significantly increased the resistance of Klebsiella pneumoniae to antimicrobials used in the treatment of urinary tract infections. The objective of this study was to determine the frequency of uropathogens and the sensitivity profile of first choice antimicrobials used in the treatment of uncomplicated urinary infections of K. pneumoniae in the community of Londrina-Pr. Materials and methods: The urine cultures were carried out in the years 2016 and 2017 by Centrolab laboratory - Central Laboratory of the municipality of Londrina. Bacteria were identified by Vitek 2® automated system (bioMerieux). Susceptibility Profile was analyzed by program SPSS 20.0. Positive male and newborn urocultures were excluded, included only positive female urocultures. The study was approved by the Ethics and Research Committee of the State University of Londrina CAAE 56869816.0.0000.5231 and authorized by the Health Department of the Municipality. Results and conclusion: 6,509 samples were analyzed, of which the highest frequency was Escherichia coli with about 5,169 samples (79%) and secondly with K. pneumoniae with 599 (9.2%), Proteus mirabilis. The antimicrobials used in the treatment of uncomplicated UTIs showed the following sensitivity profile to K. pneumoniae: Amikacin 99%, Amoxicillin/Clavulanic Acid 82.6%, Ampicillin 45%, Cephalotin 51.7%, Cefepime 91.9%, Ceftriaxone 91.3%, Cefuroxima 83.9%, Gentamicin 92.5%, Nitrofurantoin 82.8%, Piperacillin/Tazobactam 96.3%, Trimpetoprim/Sulfamethoxazole 70.9%, Nalidixic Acid 69.2%, Norfloxacin 79.8%, Ciprofloxacin 79.8%. And 9.7% were extended-spectrum beta-lactamase (ESBL)-producing K. pneumoniae. Antimicrobial resistance has become a public health problem and the use of antibiotics for treatment of ICUs has contributed to increasing this percentage in the community. Financial support or fellowship: Foundation Araucária

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

DETERMINATION OF THIOCYANATE IN URINE

204

CFSP377

ANA LUÍZA ROCHA PICANÇO; CAMILA DE LIMA SILVA; RENAN PEREIRA DE LIMA; SANDRA MARIA ROCHA; ARLANDIA CRISTINA LIMA NOBRE DE MORAES. Universidade de Fortaleza - Unifor, Fortaleza - CE - Brazil. Introduction and objective: Thiocyanate is a normal component of mammal tissue and fluid. It is found, in low concentrations, in both cassava and cheese. High levels of thiocyanate are related to smoking. Part of the cyanate absorbed by the body is excreted, unaltered, through the lungs. However most of it is metabolized to thiocyanate by the enzyme rhodanase. This, in turn, is mainly excreted through urine. In this study we tried, through the spectroscopic method, to measure thiocyanate levels in the urine of the patients at the “Núcleo de Atenção Médico Integrada (NAMI)”. Materials and methods: This is an experimental, quantitative and descriptive study. Thiocyanate levels were measured by the spectroscopic method through the reaction between thiocyanate found in the urine samples and bromine. This reaction formed cyanogen bromide, which, in turn, formed a colored complex with pyridine. It was possible to read this complex at a 520 nm wavelength. The measurement was performed on the urine of 19 patients from the “NAMI” at “Universidade de Fortaleza”. This study was approved by the Research Ethics Committee Involving Human Beings at the “Universade de Fortaleza”, certificate No. 040/08. Results and conclusion: The results were compared to reference data. They showed that 42% of patients displayed thiocyanate levels above 4mg/mL. Of these patients, 100% were smokers. This way it is possible to suggest that they were being exposed to some source of cyanide. Therefore, measurement of thiocyanate levels is considered important as a biomarker for the evaluation of smoking. The measurement must be taken with a questionnaire which can exclude, or not, other possible sources of cyanide.

THE LUNAR CYCLE AND SUICIDE

CFSP378

ERICK DE SOUSA SILVA; JAIR MARQUES JUNIOR; JULIANA MARQUES DOS SANTOS; ELIANE APARECIDA SUCHARA. UFMT, Barra do Garcas - MT - Brazil. Introduction and objective: The lunar influence on suicide became a widely discussed subject around the world. Suicide is a worldwide problem which is currently among the leading causes of death in the world. The objective of this study was to analyze the occurrence of suicide cases between 1999 and 2016 in the city Barra do Garças and correlate them with the lunar cycle. Materials and methods: A descriptive and cross-sectional epidemiological study was carried out. All the cases registered as suicides at the Institute of Forensic Medicine (IFM) in Barra do Garças, Mato Grosso, were included. Results and conclusion: The male gender had a greater number of cases in the waxing moon (33.3%), while in the female gender the lunar phase most evidenced was the waning moon (37.5%); As to age, for adolescents, the lunar phase most present was the waning moon (50%), for adult it was the crescent moon (33%), and for the elderly also the waning moon (368%). As to the most commonly used cause, asphyxia (32.3%) and head trauma (36.8%) were seen on the waxing moon, the different types of shock (36.3%) were found equally on the waxing moon and full moon, and exogenous intoxications (43.2%) occurred more frequently on the waning moon, where the main toxic agents were pesticides (60.9%). In relation to the lunar cycle and the month of occurrence, it was observed that on the waxing moon the month with the highest occurrence was November (17%), on the full moon the month was April (20%), on the waning moon the months with the highest case numbers were August, September and October (13,1%) and the new moon in the month of April had the highest number of suicides (21.7%). As for the day of the week, Sunday was the day of the week with the highest incidence (17.5%) and 41.6% occurred on the waxing moon. In this way, it is concluded that the male gender on the waxing moon and the female gender on the waning moon in adult age group were more likely to commit self-harm. So to assume a relationship between the lunar cycle and suicide more projects in the area are necessary.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

TiO2 AND ZnO NANOPARTICLES IN PHOTOPROTECTORS

205

CFSP379

JULIANA MARQUES DOS SANTOS; ERICK DE SOUSA SILVA; ELIANE APARECIDA SUCHARA. UFMT, Barra do Garcas - MT - Brazil. Introduction and objective: Nanotoxicology is an emerging area that grows in the same proportion in which investments are inserted in the nanotechnology area, among them, nanocosmetics. One of the main characteristics of nanoparticles is their absorption advantage over larger particles. Titanium dioxide (TiO2) nano has been correlated with the ability of chromosome segregation, cytokinesis, centrosomic duplication, and functional regulation of protein mitotic Serine/threonine-protein kinase (PLK1) and the production of reactive oxygen species (ROS). ZnO nanoparticles have the potential to lead to a significant reduction in mitochondrial dehydrogenase activity in human lymphocyte cells and ROS generation. Effects on human epidermal cells are observed even at low concentrations and potential genotoxic and cytotoxic effects. Thus, the present study sought to evaluate the use of potential nanoparticles of TiO2 and ZnO in photo protectors commercialized nationally. Materials and methods: Regarding the use of TiO2 and ZnO in nano form, the labels of photoprotectors marketed in drugstores, cosmetics stores and resellers were analyzed. The presence of a label appeal was also evaluated. Results and conclusion: Of 25 labels, 16 products contained only TiO2 in the composition, one contained only ZnO, two contained an association of TiO2 and ZnO, and in six products there were none of the physical filters studied. As for the label’s appeal, only three products presented data on the use of TiO2 and ZnO nanoparticles. According to RDC No. 7/2015, sunscreens are subject to registration with ANVISA, because these products have specific indications, however, the actual amount of photoprotectors registered with nanoparticles is not observed on this platform. Even with exponential growth of nanocosmetics, there is no clear information on the use of nanoparticles in the labels evaluated. The decision about the advertising and labeling of the products belongs to the manufacturer. Considering that nanoparticles are increasingly used in cosmetics, more studies are needed to know the long-term risks. CFSP380 TOXICOLOGICAL STUDY OF THE DRY GROSS EXTRACT FROM THE FRUIT OF THE JURUBEBA (SOLANUM PANICULATUM L.) THROUGH TESTS WITH ARTEMIAS SALINAS LEACH JOSÉ CARLOS BERNARDO DA SILVA FILHO1; JOICE LUIZA PEREIRA DA SILVA2; IZABELLA CINTHIA TÔRRES DE VASCONCELOS2; BRUNNA LARISSA DE SOUZA MELO FERREIRA2; GIOVANNA GABRIELLY ALVES DA SILVA FRAGA2; RISONILDO PEREIRA CORDEIRO2. ASCES-UNITA, Jurema - PE - Brasil; 2ASCES-UNITA, Caruaru - PE - Brazil.

Introduction and objective: The Solanum paniculatum L. is part of the Solanaceae family, popularly known as Jurubeba. It presents various therapeutic activities such as anti-inflammatory, anti-diabetic and diuretic activity. From this, it was aimed to perform toxicity test using the dry gross extract of the pine’s fruit. Materials and methods: At the Lethal Concentration (CL) Study, the eggs of the Artemia salina were incubated for 48 hours aiming at larval hatching. They were separated into 7 different groups containing 12 metanauplius each. One group received the control solution (marine water) and the other 6 groups received different concentrations (1000, 750, 500, 250, 100, and 50 µg/mL) of the dry gross extract from the jurubeba fruit. The Artemias were placed under artificial light for 24 hours and after this period, notes were made, counting the numerical value of live and dead larvae. This assay was performed in triplicate. Results and conclusion: It was observed that the number of dead Artemias were proportional to the increase of the tested concentrations. The results of the Lethal Concentration (CL50) were 580,138 µg/mL which demonstrates that it could be moderately toxic, through this result, further pre-clinical toxicological tests are required to confirm the obtained results. Financial support or fellowship: ASCES-UNITA

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CFSP381 TOXICOLOGICAL STUDY OF THE DRY GROSS EXTRACT FROM THE STEM OF THE CNIDOSCOLUS QUERCIFOLIUS POHL (FAVELA) USING ARTEMIA SALINA L. ALANE ALEXANDRA DA SILVA OLIVEIRA1; IRTHYLLA NAYALLE DA SILVA MUNIZ2; BRUNNA LARISSA DE SOUZA MELO FERREIRA2; YASMIM DAYANE LEAL PAIXÃO2; LETÍCIA VIEIRA DOS SANTOS BESERRA2; ARQUIMEDES FERNANDES MONTEIRO DE MELO2. Centro Universitário Tabosa de Almeida ( ASCES- UNITA), Caruaru - PE - Brazil; 2ASCES-UNITA, Caruaru - PE - Brazil.

Introduction and objective: The Cnidoscolus quercifolius Pohl (Favela), presents properties for grazing, besides its popular uses as healing, anti-inflammatory and antibiotic. The presence of stinging thorns containing hydrocianic acid means that its toxicity is similar or greater than most o cyanogenic plants from the Brazilian Northeast Region. This study aimed to determine the toxicity of the Cnidoscolus quercifolius (Favela) using the dry gross extract from its stem. Materials and methods: The eggs of the Artemia salina were incubated during 48 h so that would be a larval hatching (Metanauplius), those were separated into 7 different groups with 12 larvae in each one of them. The first group as a control (marine water) and the following groups with different concentrations (50µg/mL 100µg/mL, 250µg/mL, 500µg/mL, 750µg/mL and 1000µg/mL) of the dry gross extract from Favela, in triplicate. The Artemias were submitted to artificial lighting for 24h. The number of live and dead larvae was counted and the data tabulated in the Microcal Origin 4 program. Results and conclusion: The number of dead Artemias was proportional to the increase of tested concentrations, the number being higher or equal in the last concentrations. During the process, the swimming of the Artemias was similar to the control. The results of the Lethal Concentration (LC50) were 7.302,32086 µg/mL, which demonstrates that it is practically non-toxic. It is concluded that the execution of other pre-clinical toxicological tests is needed to confirm the obtained results. Financial support or fellowship: ASCES-UNITA

Brazilian Journal of Pharmaceutical Sciences

Area 4 Management and Regulatory Aspects

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

ADVANCES IN PHARMACOVIGILANCE LEGISLATION IN BRAZIL

209

CFSP382

FABIANA ROSSI VARALLO1; CAROLINA DAGLI HERNANDEZ2; PATRÍCIA DE CARVALHO MASTROIANNI1. Department of Drugs and Medications, School of Pharmaceutical Sciences, Unesp-Univ Estadual Paulista, Araraquara - SP Brazil; 2School of Pharmaceutical Sciences, University of São Paulo, São Paulo - SP - Brazil. 1

Introduction and objective: Brazilian health legislation on drug surveillance has only been available for four decades and many pharmacovigilance measures have been implemented so far, aiming to improve the risk-benefit of drugs and proactively detect drugrelated problems. In this study, we aimed to assess the evolution of the Brazilian legislation on pharmacovigilance over the years. Materials and methods: A search was performed in the legislation databases of the National Health Surveillance Agency (ANVISA) and in official websites using the keywords “drug safety”, “marketing authorization holders”, “pharmacovigilance” and “patient safety”. The following variables were defined: year, scope, type of legislation, to whom it is applied, and what is regulated. The evolution of concepts, competences and responsibilities in pharmacovigilance of each sector involved were analyzed. Results and conclusion: Sixty-two (62) documents published between 1976 and 2015 were identified. Advances in the assessment of health technologies only occurred after ANVISA launched the Sentinel Network in 2009. Also, although recent, the regulatory advancements in pharmacovigilance in Brazil are equivalent to international practices. There is still a lack of regulation for biosimilars and veterinary medicines, of agility in reporting non-serious risks to manufacturers and health care services, and of encouragement to report technical complaints and quality deviations. It is necessary to encourage and develop strategies to decentralize pharmacovigilance actions. (Pharmaceut Reg Affairs 5:164) Financial support or fellowship: Grant #2014/12681-2; grant #2013/10263-9, São Paulo Research Foundation (FAPESP). CFSP383 ADVERSE EVENTS NOTIFIED TO THE BRAZILIAN HEALTH SURVEILLANCE NOTIFICATION SYSTEM (NOTIVISA) IN BRAZIL, FROM 2006-2013 MAURO BIATO RODRIGUES BARBOSA1; TATIA TIE YUHARA2; LARISSA COMARELLA3. Uninove, São Paulo - SP - Brazil; 2Uel, Londrina - PR - Brazil; 3Ufpr, Curitiba - PR - Brazil.

Introduction and objective: Pharmacovigilance refers to activities relating to identification, evaluation, understanding and prevention of adverse effects or any problem related to medications. To carry out pharmacovigilance activities, the Brazilian Health Surveillance Notification System (NOTIVISA) should be used. This work aims to identify the importance of the use of NOTIVISA to promote the rational use of medicines. Materials and methods: Descriptive study of NOTIVISA notifications for the period 2006-2013. Results and conclusion: The data obtained showed that the notifications of adverse event by product/reason (medical articles, blood or component use, cosmetics, sanitizers and medications) increased significantly in the period studied. There was an increase in the number of reports of adverse events for use of medicines, blood and blood products, from 2007. Several factors may contribute to the development of adverse events with medications, so notification is of fundamental importance for systems such as NOTIVISA, which act as essential tools for the provision of information about health products. This information may affect the process of drug consumption, the demand for consultations and health procedures, and foster the level of social sanitary awareness that would act as a barrier to the inappropriate use of these products. It was concluded that NOTIVISA is important in monitoring the quality and use of products under sanitary surveillance, contributing as a further tool to promote the rational use of medicines, through monitoring, research and availability of results related to Medicines Problems and Adverse Drug Reactions.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

ANALYSIS OF THE PROFILE OF DRUG INTOXICATIONS IN BRAZIL, FROM 2008 TO 2012

210

CFSP384

MAURO BIATO RODRIGUES BARBOSA; KARINE GARGIONI PEREIRA CORREA DE MELLO; MEIRE ROBERTA B. MENDES LEDESMA. Uninove, São Paulo - SP - Brazil. Introduction and objective: Drug intoxication can be defined as a set of signs and symptoms produced by a drug when it is ingested, inhaled, injected or comes in contact with the skin, eyes or membranes above the therapeutic dose and can be classified as acute or chronic. It is a problem of great relevance for public health. The objective of this study is to analyze the profile of drug intoxications in the country, from 2008 to 2012, considering the following conditions. Materials and methods: The data were searched for through the Fiocruz/Sinitox website, restricting the survey to the period from 2008 to 2012, considering the following situations and conditions: region of Brazil, age group, gender, and some circumstances. Results and conclusion: This study showed that during the years 2008 to 2012, 138,376 cases of drug poisoning were registered, with the female sex being the sex most affected when we consider cases of intoxication, and children under 05 years of age represent the most affected age group. Among the circumstances in which intoxications occurred suicide appears first, followed by the individual accident. Administration and self-medication errors occupied a prominent place in the cases, requiring attention. The importance of the pharmacist at the time of dispensing the drug becomes clear. He/she provides a correct pharmaceutical assistance which includes guidance on the rational use of medicines, treatment benefits, side effects and harm caused by incorrect use of medicines to reduce cases of drug poisoning. CFSP385 BIODEGRADATION ASSESSMENT OF FLUOXETINE HYDROCHLORIDE AND ITS MOST CONSUMED COMPOUNDS IN BRAZIL: COMPARISON OF RESULTS OBTAINED IN RESPIROMETRY TEST AND FROM THE USE OF QSAR’S MODEL (ECOSAR) SUZETE MARIA LENZI CAMINADA1; MIRIAM MOREIRA BOCCHIGLIERI2; WANDERLEY DA SILVA PAGANINI2. Faculdade de Saúde Pública/USP, Jaguariuna - Brazil; 2Faculdade de Saúde Pública/USP, São Paulo - SP - Brazil.

Introduction and objective: Due to the current concern of the scientific milieu with the presence of environmental micro pollutants (pharmacos), the possible deleterious effects that these compounds can cause and their continuous replacement in the environment, the risk to aquatic organisms, as well as to human beings of chronic exposure, these substances have been the object of several studies. The current work approaches aspects of Fluoxetine hydrochloride Biodegradation, considerations about the compound structure, based on the data of the QSAR’s model (ECOSAR), identifying the probable compartments and the respective extent of their distribution. Materials and methods: The work was developed from bibliographic research to identify the most consumed pharmacos in Brazil, in the last two years, and data collection on the effectiveness of removal in Sewage treatment plants. Respirometry tests were carried out based on the modified test by Gledhill, 1988- (IBAMA), as well as considerations about the structure of the compound using the QSAR’s model (ECOSAR). Results and conclusion: The results showed that the drug in study was partially degraded by the organisms in the test system, with a maximum removal of, approximately, 27%. According to the data obtained by the ECOSAR program, the bioaccumulation factor calculated was of BFC= 262.1, indicating that the compound presents a high cumulative potential. The behavior of this pharmaco in wastewater treatment presents a total removal of 32.4%, of which, 32% adhered to the mud, and 0.4% biodegradable by the activated mud. The biggest amount, 67.6% is found soluble in treated effluent which is dumped downstream from the treatment station. The model of fugacity previewed by the program indicates that around 11% of this molecule can be found dispersed in the water, and 84% in the soil with a half-life of 2 months. Continuing the studies, the most consumed pharmacos in Brazil, will be evaluated according to the QSAR’s model (ECOSAR), in order to evaluate the behavior of these compounds in the several environmental matrices.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

CHARACTERIZATION OF HEALTH LAWSUITS IN THE CITY OF REGISTRO/SP

211

CFSP386

EUCLIDES DA SILVA PEREIRA NETTO1; JAQUELINE DA SILVA LIMA1; REYNALDO CARNEIRO DE SOUZA NETO1; CECILIA ARMESTO2; LEONÍ ADRIANA DE SOUZA1; MARIANA DONATO PEREIRA1. Faculdades Integradas do Vale do Ribeira, Registro - SP - Brazil; 2Acfaculdades Integradas do Vale do Ribeira, Registro - SP - Brazil. 1

Introduction and objective: The increase in lawsuits requesting medicines, nutritional therapy, procedures and other products for health has been of concern in the three spheres of management of the Unified Health System. The objective of this study was to analyze the impact of legalization of health care in municipal records in terms of public spending, evaluating the profile of applicants who plead in court the cost of treatment, examination, procedure or medication. Materials and methods: To do this, data obtained through the Registration Department of Health on cases filed from 2009 to 2015 were analyzed. Results and conclusion: We identified 10 cases against the registry of City Hall, Sao Paulo. The monthly costs of treatment ranged from R$ 148.15 to R$7,207.08, making clear the commitment of the public budget. The number of cases requesting drugs in the city may seem small at this time, however the amount spent per month is approximately R$ 13,433.00. As the population of the region is considered one of the poorest in the state of São Paulo, having little access to information and often unaware of their rights, thus being able to justify the number of cases in the period. Thus, the health lawsuits represent a challenge for many municipalities, their characterization Registry contributes to the definition of viable intervention strategies to assist the manager in decision making and policy development that meet the real needs of local people. CFSP387 COMPARATIVE ANALYSIS OF THE BRAZILIAN AND BRITISH HEALTH SYSTEMS: SUS AND NHS ROLES ON GRANTING PRIMARY HEALTH CARE TO THE POPULATION MARIA APARECIDA NICOLETTI; TAISSA DE MATTOS FARIA. FCF-USP, São Paulo - SP - Brazil. Introduction and objective: The Brazilian Unified Health System (SUS) was created inspired by the principles of universality, integrality and equity, the same that guided the National Health Service (NHS). Both systems are public, provide free access and the financing is substantiated by tax collection. However, regarding the primary health care, in the NHS, primary health care is the main focus and covers almost the entire population. In the SUS, in turn, less than 50% of the population is covered with effective primary health care services. Thus, this study aims to critically compare the two systems using an evaluation of the primary health development stage. Materials and methods: A comparative analysis was carried out between the two systems through regulatory documentation provided by the two countries that base the structuring of health care systems with a focus on basic care to verify the similarities and differences present. Results and conclusion: Although the SUS and NHS were guided by the same principles, they also had to adapt to their socioeconomic, epidemiological and historical contexts over the years. In the SUS, there has been a progressive increase in municipalities’ and states’ participation in managing and financing the system, along with a decrease in federal involvement. In the NHS, the financing remained centered in the national sphere, with regional offices responsible for executing and contracting their health care services. Also, regarding the provision of primary health care, a regionalization of this responsibility can be observed in both. However, in the NHS, this transferring has been accompanied with knowledge sharing and monitoring from the national sphere, unlike the SUS, which often does not have local managers with sufficient qualification to handle the health care in their regions. Therefore, the management of a universal health care system requires managers with good administrative skills in order to fully guarantee their principles.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

COMPOUNDING LABORATORY ADEQUACY OF A PUBLIC UNIVERSITY HOSPITAL

212

CFSP388

ATHILA WESLEY LIMA LACERDA; NATALHA NAYANE DE OLIVEIRA PINHEIRO; SANDNA LARISSA FREITAS DOS SANTOS; ANTÔNIA IANA MUNIZ DE MESQUITA; PATRÍCIA FERNANDES DA SILVEIRA. Universidade Federal do Ceará, Fortaleza - CE - Brazil. Introduction and objective: Compounding resolutions in Brazil have undergone several adjustments, it being difficult for the public sector to monitor such evolutions on a structural and organizational level. This work aims to show the adequacy of a Compounding Laboratory – LABMA - at the University Hospital of the Federal University of Ceará, Fortaleza, in accordance with the current resolution, RDC 67/2007 and 87/2008. Materials and methods: A descriptive, exploratory study where data were obtained by researchers having direct contact with the situation under study. The direct source was the elaboration of a plan of action based on the application of the self-inspection proposed by the legislation and the descriptive research of data analyzed through the cause-effect diagram through the mobilization of pharmacists, residents and technicians. Results and conclusion: From the observation, several items in non-compliance with the legislation could affect the commitment of the activities related to the sector, all the necessary documents for the adequacy of the same were updated. Standard Operational Procedures (SOPs), daily activity schedule, manipulation flows, input specification sheets, and reports were updated and available on the hospital intranet. Training of the team and dissemination of the formulas of manipulation in the institution was also undertaken by the professionals. Through the action plan, better organization of the sector was observed. It is worth mentioning that the laboratory is a reference for other institutions, considering that it is a field of curricular internship and multiprofessional residency. Adapting this sector to these activities makes the environment one of economy to hospital costs. Financial support or fellowship: UNIVERSIDADE FEDERAL DO CEARÁ, CE, BRAZIL.

DOES EXPOSURE TO TOXIC SUBSTANCES DURING PREGNANCY INCREASE ANXIETY?

CFSP389

ELEOMAR VILELA DE MORAES1; THAISA CIMARDI DELUQUI2; THAÍS WÉRICA TEIXEIRA2; BRUNA DO NASCIMENTO GODOI2; ANDIARA LUIZA CARVALHO MONTEIRO2; KEILA ARAUJO MONTEIRO2; OLEGÁRIO ROSA DA TOLEDO2; FLÁVIA LÚCIA DAVID2; MARIZA MARTINS AVELINO1. Universidade Federal de Goiás, Goiânia - GO - Brazil; 2Universidade Federal de Mato Grosso, Barra do Garças - MT - Brazil.

Introduction and objective: Exposure to toxic substances in unacceptable quantities is a public health problem worldwide, especially when it occurs during pregnancy. The result can be harmful for both mother and fetus. Thus, we evaluated the prevalence of contact with toxics and anxiety agents in pregnant women. Materials and methods: It was a descriptive cross-sectional study that included 178 puerperal attended at a reference public maternity hospital in Barra do Garças - MT, Brazil. Data were collected between August 2016 and June 2017. For the diagnosis of anxiety, the HAD self-assessment scale was used. The research was approved by CEP/UFMT/2015 certificate nº 975.413. Results and conclusion: The partial sample consisted of pregnant women with an average age 25±5 years, of whom 41.14% had symptoms of anxiety. In total, 6.29% of them had contact with toxic chemicals such as paints, formaldehyde, and thinner. Of these, 4.57% were exposed to insecticides and 2.84% to agrotoxins. There was no association between symptoms of anxiety and exposure to toxic chemicals PR=1.6636 (0.7538-3.6714), to insecticides RP=1.58 (0.64-3.91) or to agrotoxins 0.98 (0.47-2.02), however, this study has shown that exposure levels of pregnant women are unacceptable. Financial support or fellowship: CAPES

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

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CFSP390 EVALUATION OF GOOD STORAGE AND DISTRIBUTION PRACTICES FOR PHARMACEUTICALS IN THE MUNICIPAL PHARMACEUTICAL SUPPLY CENTER NICOLE ZALLI CIBELLA 1; JOSÉ FERNANDO RINALDI DE ALVARENGA 2; PATRÍCIA DE CARVALHO MASTROIANNI2. Faculdade de Ciências Farmacêuticas de Araraquara - Unesp, São Paulo - SP - Brazil; 2Faculdade de Ciências Farmacêuticas de Araraquara - Unesp, Araraquara - SP - Brazil. 1

Introduction and objective: Good storage and distribution practices can impact pharmaceutical quality, loss reduction and cost minimization. Objective: To evaluate compliance with good storage and distribution practices for pharmaceutical products in the Araraquara Municipal Pharmaceutical Supply Center. Materials and methods: This is a descriptive study, with a qualitative-quantitative approach such as data gathering (action research). An audit roadmap was drawn up based on current legislation and, through observation, interviews and documentation analysis, the compliance and noncompliance of Pharmaceutical Supply Center processes, management and infrastructure were evaluated, according to the sanitary regulations related to Good Storage and Distribution Practices for pharmaceutical products. Non-compliant items were classified according to their urgency of adjustment, their risk to safety and to service quality. Results and conclusion: A total of 299 items of good storage and distribution practices were evaluated, and of every four items, three were classified in compliance with regulations, evincing the commitment of the Pharmaceutical Supply Center management. Only 16 items (5.3%) were not detectable because they were not directly Pharmaceutical Supply Center activities. Fifty-eight (19.4%) non-compliant items were identified and the majority referred to the structure (26 items [8.7%]). Among the non-compliant items, 16, 31 and 11 items of high, medium and low urgency were classified, respectively. The audit contributed to the resolution of 12 non-compliant items, such as the amendment and adjustment of the Standard Operating Procedures and the Good Practices Manual. Financial support or fellowship: Araraquara Pharmaceutical Assistance and PRÓ-SAÚDE/PET-SAÚDE-Pharmaceutical Assistance/MS CFSP391 EVALUATION OF THE IMPACT OF THE BRAZILIAN COMMON NAMES (DCB) IN THE REGULATORY FRAMEWORK OF THE NATIONAL SANITARY SURVEILLANCE AGENCY (ANVISA) RAQUEL PEREIRA GUIMARÃES1; MÔNICA VALERO DA SILVA2. Anvisa, Brasília - DF - Brazil; 2Universidade de Brasília, Brasília - DF - Brazil.

Introduction and objective: DCB are the names of the drug or pharmacologically active principle approved by the federal agency responsible for health surveillance. The objective of this study was to describe the main impacts of the DCB in ANVISA and also in the SUS. Materials and methods: A descriptive statistical analysis was performed by means of graphs obtained from the information obtained from the Resolutions of the Board of Directors, Resolutions, Decrees, and Normative Instructions published by ANVISA from February 1, 1999 to December 31, 2015. A survey was made of how many regulations were in force, how many were in force with amendments and how many had already been repealed. The percentage of DCB citation was also measured in the analyzed macro-themes. Results and conclusion: Of the 109 regulations classified within the topic Pharmacopoeia, 50 mention DCB. Most of the DCB Resolutions were repealed as they were changes to the list of DCB. The DCB are cited in 17.5% of the regulations of medicines, in 26% of the regulations of active pharmaceutical ingredients. DCB is cited in almost 50% of the published regulations within the macro-theme Pharmacopoeia, we can notice the relevant participation of this product among the products of the Brazilian Pharmacopoeia. They are extremely relevant, both from the sanitary point of view, and from the point of view of public health, considering that the SUS prescription can only be made using the DCB name. Therefore, it is necessary that the list of DCB and the rules for their establishment must be constantly revised and updated so that they are references to the other global nomenclature systems and effectively meet the needs of the SUS.

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

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CFSP392 EVALUATION OF THE PHARMACIST’S UNDERSTANDING OF GOOD MANUFACTURING PRACTICES SHÉRIDA MARIA OLIVEIRA DE SOUSA1; ANDRÉA BESSA TEIXEIRA2; TICIANA PRACIANO PEREIRA2. Graduate in Pharmacy from Faculdade de Ensino e Cultura do Ceará - Faece, Fortaleza - CE - Brazil; 2Professor of Faculty of Pharmacy at Faculdade de Ensino e Cultura do Ceará - Faece, Fortaleza - CE - Brazil. 1

Introduction and objective: The Good Practices of Manufacturing Compounding Pharmacy (BPMF, in Portuguese) follows the Resolutions from the Board of Directors 67/07 and 82/08, which establishes the minimum requirements for the practice of compounding activities by magistral and workroom preparations aiming at the quality of products and most importantly customer safety. The objective of the present study was to identify the pharmacist profiles acting in compounding pharmacies as well as the level of knowledge of items covered by the “BPMF”. Materials and methods: A cross-sectional and descriptive study was carried out, with a quantitative approach utilizing a questionnaire presented to 20 professionals of pharmacology in Fortaleza-CE. Results and conclusion: Among the interviewees, 35% were male and 65% were female, graduates within the last 5 years (55%) from public institutions (65%). The results observed that despite 90% of the interviewees not having post-graduations in magistral pharmacology, the majority of the professionals (80%) affirmed that as a result of non-compliance of received prescriptions (dosage, incompatibility or interaction), they would confirm with the prescribing professional, and in its absence, prescriptions would not be filled. Another relevant concern in the study relates to the responsibility of storing substances with low therapeutic rate, in which 60% affirmed that only the pharmacist is responsible, and the other 20% would not have the need of responsible personnel to support. Concerning the aspects of quality control of raw materials and products, 90% confirmed that the pharmacist is responsible, however, just 45% presented knowledge of the quality required by “BPMF”. Therefore, it is possible to suggest the necessity of the pharmacist professional to carry the knowledge updated on technical and scientific areas, mainly if it relates directly to current legislation. CFSP393 GLUTEN IN STANDARD MEDICINES IN A REFERENCE MATERNITY: A PHARMACOVIGILANCE STUDY ATHILA WESLEY LIMA LACERDA; SANDNA LARISSA FREITAS DOS SANTOS; NATALHA NAYANE DE OLIVEIRA PINHEIRO; EUGENIE DESIRÉE RABELO NÉRI. Universidade Federal do Ceará, Fortaleza - CE - Brazil. Introduction and objective: Pregnant women with celiac disease are deficient in nutrient absorption, which can lead to complications for the baby and the mother. Resolutions 137/2003 and 26/2015 warn about allergens that can cause damage, such as gluten that cause irritation to the mucosa of the small intestine. Objective: Obtaining data from the contact with the manufacturers of standardized medicines at the Assis Chateaubriand Maternity School (MEAC), Fortaleza, Ceará, regarding the presence and control of gluten in its composition. Materials and methods: A cross-sectional and descriptive study, with a quantitative approach was carried out in May 2017. Information was obtained by contacting MEAC suppliers through e-mail and telephone, and classified for confirmation and presence of gluten. The sample was 160 drugs. Results and conclusion: 55 manufacturers were consulted on 160 drugs. 31 (56.36%) manufacturers provided information corresponding to 94 (58.75%) drugs, which were subdivided into 5 categories: With gluten and with allergen control drugs, 17 (18.09%); With gluten and without control of allergen, 3 (3.19%); Gluten-free and with allergen control, 38 (40.43%); Glutenfree and without allergen control, 31 (32.98%). In addition, 2 (2.13%) drugs had no gluten in their composition, but they shared machinery and 3 (3.19%) drugs were no longer produced by the manufacturers contacted and they were missing at the institution. 24 (43.63%) manufacturers did not return contact, corresponding to 72 (45%) drugs, which were included in the “information not provided” category. It is concluded that it is necessary to pass on more complete information on possible allergens in the composition of the medicinal products by the manufacturer for an effective pharmacovigilance. Financial support or fellowship: MEAC, UFC, EBSERH

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

GLUTEN PRESENCE IN EXCIPIENTS OF ORAL MEDICINES IN A SCHOOL MATERNITY

215

CFSP394

NATALHA NAYANE DE OLIVEIRA PINHEIRO; ATHILA WESLEY LIMA LACERDA; SANDNA LARISSA FREITAS DOS SANTOS; JÉSSICA BEZERRA DA COSTA; EUGENIE DESIRÉE RABELO NÉRI. Universidade Federal do Ceará, Fortaleza - CE - Brazil. Introduction and objective: Gluten may be present in oral drug excipients. Formulations with this protein may cause risks to pregnant women and newborns who may present with celiac disease or predisposition. Objective: Verifying the presence of gluten in oral medications standardized at the Maternity School Assis Chateaubriand (MEAC), determining the most prevalent excipients and listing the drugs according to annual consumption. Materials and methods: A cross-sectional and descriptive study was carried out with a quantitative method in May 2017. Information was obtained through consulting information leaflets, records on the National Agency for Sanitary Surveillance (ANVISA) website and collected information from MEAC’s vendor manufacturers through e-mail and telephone. Annual consumption of the medicines was consulted in the data provided by the Pharmaceutical Supply Center. 154 standardized drugs were analyzed in the institution. Results and conclusion: Of the 154 evaluated medicines, 46 (29.87%) presented gluten, in which starch, in its several variations, was the most prevalent excipient. Of the drugs with the presence of gluten in their composition, the most consumed were cephalexin (360.75), levothyroxine (91.33), diazepam (73.58), pyrimethamine (53.42), warfarin (33.33), Phenobarbital (26.92). It is concluded that gluten is present in many medicines of considerable consumption in the referred maternity, that is why is necessary for pharmacists to adopt this knowledge, in order to correctly orient the prescribers and patients. Financial support or fellowship: MEAC, UFC, EBSERH.

HEALTH RISK MANAGEMENT: PHARMACOVIGILANCE AS AN ALLY FOR PATIENT SAFETY

CFSP395

ALAN MAICON DE OLIVEIRA1; VALÉRIA ALBUQUERQUE VAZ RODRIGUES2; BEATRIZ ALESSI MINTO3. Universidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquara - SP - Brazil; 2Hospital de Ensino Santa Casa de Fernandópolis, Fernandópolis - SP - Brazil; 3Fundação Educacional de Fernandópolis (Fef), Fernandópolis SP - Brazil. 1

Introduction and objective: Adverse Drug Reactions (ADR) are defined as responsible for hospitalization, they may even lead to death and can threaten patient safety. In Brazil, there is still a shortage of epidemiological data on these episodes and an approach still insufficient. In face of the above, the present study aimed to describe the performance of a pharmacovigilance department, and to contextualize the benefits for patient safety. Materials and methods: A cross-sectional study was carried out in a Hospital School in the northwest of São Paulo State. Through the Anvisa Notivisa system, the notifications characterized as ADR and technical complaints were computed. In cases of ADR, a severity criterion was established, as well, as for the patients that were involved, (sex and age). Notifications that were not finalized, with status pending completion or with incomplete data, were excluded. The period of analysis was from June 2012 to July 2014. Results and conclusion: A total of 151 (84.8%) notifications of possible ADR and 27 (15.2%) of technical complaints were recorded. Prevalence of episodes of ADR classified as moderate (70.2%). Among the patients who suffered these episodes, the majority were female (62.9%) and the average age (years) was 47.4 ± 22.9. From the reports, it was possible to perform interventions to avoid potential undesirable outcomes, therefore, it contributes to health care security. Pharmacovigilance has been shown to be indispensable in the hospital environment for a safe scenario for patients.

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CFSP396 KANBAN SYSTEM IMPLEMENTATION TO IMPROVE THE MANAGEMENT OF SOLID UNIT DOSE (SUD) STOCKS IN A TEACHING HOSPITAL ANA PAULA CALLEJO DE SOUZA; RICARDO ALEX DE SOUSA; SUZANA SAYURI KIMURA; CECÍLIA RONDINELLI; MARIA CRISTINA SAKAI. Divisão de Farmácia do Hospital Universitário da USP, São Paulo - SP - Brazil. Introduction and objective: Kanban is a Japanese term meaning “signal” used to indicate when is time to order more, from whom, and in what quantity. The objective of this work was to describe the use of Kanban to manage SUD stocks in HU-USP. Materials and methods: The production batch size (PBS) for each SUD was determined to quantify the number of cards of the Kanban board. It was calculated by SUD consumption from April/2016 to April/2017, the average was calculated by weekly consumption and adjusted according to the amount of tablets in the package. To calculate the total number of cards a formula was used based on daily demand, daily production time (PT), stocking time (ST) in the Dispensing Area (DA) and Pharmacy Supply Center (PSC) and safety stock (SS) divided by the PBS. Results and conclusion: The study resulted in a visual color-coded board with 759 cards. The calculated PT was 1.03 minutes, the ST was determined as 7 days in the DA and 10 days in the PSC and 1.5 was used for the SS. The PBS varied for each item and it was between 5 and 50 units per package. A stream of incoming and outgoing of items was established between the DA and PSC, as well as a training program for the staff involved in the process. With the implementation of this system it is expected to reach the goal of producing at the same pace every day to minimize the peaks and troughs in the workload, as well as to react rapidly to fluctuating demand allowing the best inventory management, avoiding excessive stocks, waste and lack of medicines. CFSP397 MAPPING OF THE ACTIVITIES FOR THE IMPLEMENTATION AND CONTROL OF GOOD MEDICINE MANIPULATION PRACTICES USING QUALITY TOOLS YAGO TERRA DE CASTRO1; PATRÍCIA DE CARVALHO MASTROIANNI1; NICOLE ZALLI CIBELLA2. Faculdade de Ciências Farmacêuticas de Araraquara - Unesp, Araraquara - SP - Brazil; 2Faculdade de Ciências Farmacêuticas de Araraquara - Unesp, São Paulo - SP - Brazil. 1

Introduction and objective: Map the activities for the observance of good medicine manipulation practices (GMPM) and organize its main inputs, outputs and connections through tools of quality. Materials and methods: This is desk-based research. Using documents, legislation and good practice guides, such as the manual of good practices of manipulation of ANFARMAG, RDC 67/2007 and RDC 17/2010. Through the ‘brainstorming’ quality tool the activities were identified, which with the use of flowcharts, were mapped and organized. 24 inputs were defined, categorized as: products, services, organization, processes, and controls, with the aid of the Ishikawa diagram. All inputs were described by 5W1H tool, being: “what” (input), “how” (description of the procedure), “who” (nature of the inputs), “when” (start/finish), “where” (sector) and “why” (purpose). Results and conclusion: The 24 inputs were: documentation, SOP, qualification and validation of suppliers, HR, equipment, sanitation and hygiene, receiving material, transport, minimum inventory, outsourced services, water, quarantine, manipulation of solids, manipulation of liquids and semisolids, control labels, packaging and labelling, sampling, quality control system, quality assurance system, dispensing/service, complaints, recall, waste management and auto inspection. It was observed the 24 entries proposals allowed connections of all activities, mapping all procedures and enabling the full deployment and control of GMPM. Financial support or fellowship: PIBIC mérito e NAF – Núcleo de Atenção Farmacêutica.

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CFSP398 PHARMACEUTICAL ACTIVITIES IN THE REDUCTION OF COSTS RELATED TO THE REPLACEMENT OF INTRAVENOUS MEDICINES FOR THE ORAL ROUTE ANA PAULA VIEIRA ARAUJO; JANBISON ALENCAR DOS SANTOS; BIANCA SILVA SVICERO; MARIA APARECIDA DE SOUZA SILVA; DANIELE HERNANDES COIMBRA MUNIZ; ADIEL GÓES FIGUEREDO JUNIOR; ANA CAROLINA MORANDI; NELMA LOURENÇO DE MATOS CRUZ. Universidade Federal de São Paulo, São Paulo - SP - Brazil. Introduction and objective: The substitution of the intravenous (IV) route with the oral route (PO) during medication administration contributes to patient safety and reductions in hospital costs. The objective of the study was to determine the cost savings related to medicines when they were transferred from IV to PO. Materials and methods: Retrospective descriptive study of Pharmaceutical Recommendations (PhRs) involving the replacement of IV route of administration with PO in a neurosurgery intensive care unit (ICU) with 9 beds from January to March 2016. We analyzed medical prescriptions and it was recommended to substitute the route of administration (IV-PO) according to the clinical condition of the patient and pharmacokinetic parameters of the drugs. These PhRs were recorded in the pharmacy database, as well as whether or not they were accepted by the medical staff. To calculate the cost related to each drug, we considered the last purchase value of the drug registered in the hospital system, multiplied by the days of treatment that the patient remained in the ICU. Results and conclusion: 33 PhRs were performed of which 19 were accepted by the medical team. The drugs involved were omeprazole, with a greater number of pathway substitutions (n=9) and a 97% reduction in treatment cost; ranitidine, dexamethasone and phenytoin with a cost reduction of 92.2%, 81.4% and 68.11%, respectively. Overall, there was a cost reduction of 93%. Despite the short study time and considering only the cost of the drug, there was a significant cost reduction when replacing IV administration routes with PO. Therefore, the pharmacist contributes considerably to the saving of resources and patient safety, provided there is analysis of parameters related to the patient and the drug. CFSP399 QUANTIFICATION OF CAFFEINE IN DECAFFEINATED COFFEE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY CARLA VALÉRIA DE MELO1; MARIA FERNANDA FERREIRA DE LIMA1; GISLAINY ISRRAIANY DA SILVA FRANÇA1; JOSÉ ROMERO BARBOSA DE CARVALHO SOBRINHO2; CARLOS EDUARDO MIRANDA DE SOUZA1. Centro Universitário Tabosa de Almeida, Caruaru - PE - Brazil; 2Centro Universitário Tabosa de Almeida .

Introduction and objective: Caffeine is a metilxantina of bright, odorless crystalline structure and bitter taste. Stimulating the central nervous system (CNS) in small doses, resulting in changing the state of wakefulness, respiratory rate and heart rate, release of catecholamines, stimulation of metabolism and diuresis, and may impair cardiac and renal function and the stress state of users. Because of this, decaffeinated products are an alternative to caffeine restriction. Objective: quantify the caffeine in decaffeinated soluble coffees and check if the level is less than 0.3 g caffeine every 100 g of soluble coffee according to RDC Resolution No. 277, September 22, 2005 by ANVISA. Materials and methods: Six soluble decaffeinated coffees were selected. Numbered 1 to 6, the products were subjected to the process of pH variation and isolation of caffeine by affinity with organic solvent, chloroform was used in this case. After this procedure, the specimens were subjected to chromatographic analysis in system of C-18 reverse phase, acetontrila mobile phase: water flow of 0.8 mL/min and read by 285 nm wavelength. The caffeine present in the samples was identified and quantified on the basis of the results of the comparative analysis under the same conditions of caffeine USP standard. Results and conclusion: According to the obtained results, it was found that brand 1 introduced coffee caffeine content of less than 0.1 g/100 g; in brands 3, 4 and 5 the content is less than 0.2 g/100 g and in brands 2 and 6 values less than 0.25 g/100 g of caffeine. With these results, it is concluded that all six brands analyzed present concentrations of less than 0.3 g/100 g of product, following the regulation of Anvisa and assuring users of the low concentration of caffeine in products.

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CFSP400 THE REGISTRATION OF MEDICINES IN BRAZIL BASED ON CLINICAL RESEARCH CARRIED OUT IN OTHER COUNTRIES. JULIANA CARVALHO ROCHA ALVES DA SILVA1; ANDRÉ ADRIANO CHAIA2. Visanco Assistência Técnica, Brasília - DF - Brazil; 2Visanco Asistencia Técnica Ltda, Brasília - DF - Brazil.

Introduction and objective: This study aims to discuss the benefits and disadvantages of drug registration in Brazil based on their efficacy and safety data in clinical research elaborated in other countries. The Agência Nacional de Vigilância Sanitária (ANVISA), regulatory agency in Brazil, presents in many of its normative that deal with registration of medicines the possibility of proving efficacy and safety data using studies elaborated in other countries. With this type of determination, it is possible to understand that the clinical trial performed outside the country does not comprehensively reflect the response that the Brazilian population may have in relation to a particular drug. Brazilians present as differential features their miscegenation, different basic diseases, outpatient needs, and nutritional schemes, among other aspects. However, it is worth mentioning that if ANVISA decides to register only medicines that have clinical studies performed in Brazil, the registration process will be more time-consuming and companies will need to invest more capital for a product registration in the country, making the Brazilian market unattractive to new drugs and technologies. Materials and methods: This project will be a narrative review. The databases used will be: Brazilian legal framework; ANVISA rules; Pubmed; Medline; Lilacs; Cochrane library; Google scholar, among others. Results and conclusion: To elaborate a scientific article and poster covering the main positive and negative impacts of this type of approach for drug registration in Brazil. In relation to this impasse, an in-depth evaluation on this topic is required in order to verify the main critical points of each situation in question, identifying possible ways and strategies to balance this scenario.

TOXICITY ASSESSMENT OF FLUOXETINE HYDROCHLORIDE BY CERIODAPHANIA DUBIA

CFSP401

SUZETE MARIA LENZI CAMINADA1; MIRIAM MOREIRA BOCCHIGLIERI2; WANDERLEY DA SILVA PAGANINI2. Faculdade de Saúde Pública/USP, Jaguariuna - SP - Brazil; 2Faculdade de Saúde Pública/USP, São Paulo - SP - Brazil.

Introduction and objective: The contamination of surface water by Pharmacochemicals has raised many concerns among scientists due to the potential of negative effects on Aquatic organisms (HENRY, 2004). Little is known about the destiny and behavior of these substances in the aquatic environment, nor is it clear which organisms are affected and to what degree. The variety of Pharmacochemicals and chemical specialties, as well as the Metabolites generated, the pharmacos excreted unaltered or in their conjugated form through feces or urine, as well as the inadequate disposal and expired medicine, indicate the need for studies related to the Eco toxicological aspects. The presence of these compounds and/or their metabolites has been researched for causing physiological and genetic changes in living organisms and because of their potential to cause illnesses in human beings. Toxicity essays with Ceriodaphania dubia were applied to assess the effects of Fluoxetine hydrochloride in aquatic organisms. Materials and methods: Preliminary assays have been made aiming to evaluate the toxicity of the study pharmaco: in its commercial shape containing 20 mg of the active ingredient/capsule, afterwards using the sample descendant from the Respirometry system in closed system (concentration 204,25 ppm), IBAMA, 1998. The toxicity assays were performed according to the method NBR 13373, ABNT, 2006. The results of CE50 were obtained through statistic method (Trimmed Sperman Karber, Hamilton, 1978). Results and conclusion: Two preliminary assays were carried out using the samples descendant from the commercial pharmaceutics and the sample descendant from the Respirometry system, CAMINADA, 2009. The test results of toxicity, considering the balance of Biodegradation process, using the test organism CERIODAPHANIA DUBIA, showed acute toxic effects, CE50=1.16 ppm (0.86-1.55) - 24 hours and 0.95 ppm (0.70-1.30)- 48 hours, showing similar results to those presented by HENRY (2004) and BROOKS (2003). Financial support or fellowship: Faculdade de Saúde Pública/USP

Brazilian Journal of Pharmaceutical Sciences

Area 5 Pharmaceutical Education

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

ANTIOXIDANT ACTIVITY OF GERANIUM MACRORRHIZUM: A NEW COSMETIC OPTION?

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CFSP402

LETÍCIA YUKARI TAMASHIRO1; SABRINA DE OLIVEIRA SILVA2; MARIANA SOARES ALVES DE SOUZA1; FERNANDA ROSSI SIQUETO3; ANDREZA FRANCISCA MENDES DA SILVA1; LUIZA MELANIE SILVA1; RAFAELA ALVES BERTOLINO 1; LETÍCIA DE FARIAS FRANCO 1; MARIA CRISTINA MARCUCCI 4; CARLOS ROCHA OLIVEIRA5; VALERIA MARIA DE SOUZA ANTUNES1. Universidade Anhembi Morumbi, São Paulo - SP - Brazil; 2Universidade Anhembi Morumbi, Guarulhos - SP - Brazil; 3Universidade Anhembi Morumbi, Itapevi - SP - Brazil; 4Centro Universitário Anhanguera de São Paulo, Campinas - SP - Brazil; 5Universidade Anhembi Morumbi, São José dos Campos - SP - Brazil. 1

Introduction and objective: In order to maintain skin balance and prevent premature aging, the use of plants and fruits rich in antioxidant compounds has been extended beyond feeding, and is also applied to cosmetics. As a new active alternative, Geranium macrorrhizum is a representative species of the Geraniaceae family, endowed with several relevant phytochemical properties. It is possible to detect the presence of tannins and flavonoids, known for their antioxidant action. Objectives: To verify through laboratory analysis the presence of phenolic antioxidant compounds in Geranium macrorrhizum. Materials and methods: A hydroalcoholic extract was produced which was submitted to a curve in HPLC, analysis of total phenols by Folin-Ciocalteu method and total flavonoids. The antioxidant action was proven through the DPPH method, using solutions in low concentrations. To evaluate the proliferation capacity, the CCD172sK (ATCC) fibroblast lineage and the salt reduction technique of MTT to formazan were used. In this sense, different concentrations of extract were added to the cultures and, after 24 hours, the reading was carried out at 570nm. Results and conclusion: The flavonoids and tannins found in the structures of the species present a potent antioxidant action, which is justified by the analyses performed and the results presented. Financial support or fellowship: The plaze was kindly provided by WNF company. CFSP403 BEST PRACTICES FOR TEACHING LABORATORIES: A RISK BASED APPROACH TO IDENTIFY ESSENTIAL PROCEDURES, BRINGING TOGETHER LAB IMPROVEMENT AND QUALITY ASSURANCE LEARNING JOICE HIROSE DE TOLEDO1; MARIA APARECIDA LOUREIRO SPÓSITO2; MATHEUS BORGHI2; LAURA DE OLIVEIRA NASCIMENTO2. Faculty of Pharmaceutical Sciences (Fcf), State University of Campinas (Unicamp), Campinas - Brazil; 2Faculty of Pharmaceutical Sciences (Fcf), State University of Campinas (Unicamp), Campinas - SP - Brazil. 1

Introduction and objective: Safety is a priority in teaching laboratories, but when it comes to assuring data and learning quality or other parameters, there are no guidelines to follow. While GLPs are too rigorous, the “customized” best practices in each learning unit vary greatly and may not guarantee the intended outcomes. Therefore, this study aims to identify teaching lab components, risks, essential procedures and prioritize actions to guide teachers, technicians and assistants. In addition, the complete work will also be used as a case study to teach quality assurance tools, such as risk assessment, best practices guidelines and auditions. Materials and methods: The teaching laboratory chosen was a physicochemical analysis lab. Risk analysis was performed by Risk Ranking and Filtering (RRF), comprising: brainstorms to identify components and possible failures; Ishikawa diagram to correlate cause with failure; risk matrix to classify risks at low, medium and high. Several papers and guidelines were used to justify risk classification. Actions were proposed for high risks. Results and conclusion: We propose that the most relevant subjects in our model lab are Human and environmental safety, equipment safety, data quality and learning quality. Therefore, the evaluated failures were lack of safety/quality. We identified 6 main factors, based on the 5 M theory (general equipment, analytical equipment, environment, material and human resources), and described the items for each factor. Further, we classified all failures and detected the high-risk ones (risk matrix). Our analysis showed that Standard Operation Procedures as a risk control tool can eliminate or minimize most risks; in addition, we will present a priority list for SOP’s at the congress, based on our risk analysis, which has the lab code of conduct at number one. We believe we have achieved a well-described model for other labs to implement and a solid case study for Quality assurance learning in chemistry/life sciences courses. Financial support or fellowship: FAPEX-Unicamp; FCF-Unicamp

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CFSP404 CIMUFS-LAG: A CONTRIBUITION OF MEDICATION INFORMATION CENTER TO PROFESSIONALS UPDATE AND RATIONAL USE OF MEDICINES MAYARA DE ALMEIDA LIMA RIBEIRO; BRUNA SOUZA HORA; JOANA MARIA SANTOS DE OLIVEIRA; KAREN HELENA DE OLIVEIRA MIRANDA; MICHELLE SANTOS DE JESUS; TAINARA OLIVEIRA CARNEIRO; ADRIANA ANDRADE CARVALHO; GISELLE DE CARVALHO BRITO; LUCIANA PEREIRA LOBATO; RAFAEL CIRO MARQUES CAVALCANTE; ELISDETE MARIA SANTOS DE JESUS; TAÍS CRISTINA UNFER. Universidade Federal de Sergipe, Lagarto - SE - Brazil. Introduction and objective: Medication Information Centers (MICs) are important tools in promoting the rational use of medicines (RUM). In the Central-South region of Sergipe medical care and education centers outside the metropolitan region are centralized in the City of Lagarto. The implementation of the MICs of the Federal University of Sergipe/Lagarto (CIMUFS-LAG), aims to optimize health professionals’ and the citizens’ access to information, promoting the RUM. Materials and methods: CIMUFS-LAG is based on the fundamental right of access to information (Article 5 of the Federal Constitution) and provides active and passive medicine information. Operated by pharmacists of the Department of Pharmacy of Lagarto and students on the Pharmacy Course, CIMUFS-LAG, in the implementation phase, are located at the University Hospital of Lagarto, and uses primary, secondary and tertiary technical references for the information. Results and conclusion: The MICs information provided results in a positive impact on patient care, supporting decision making about drug therapy and identification or prevention of adverse events. Until now, six newsletters have been published and distributed, all of which were used in actions developed in partnership with EBSERH (2nd National Health Partnership/EBSERH) and with UFS (“A Day for Health at UFS 2017”). The implementation of an MIC in a hospital setting with a focus on health professionals and the community becomes a strategy for improving pharmacotherapy. Financial support or fellowship: Pibix - UFS, EBSERH CFSP405 COMPARING INFORMATION NEEDS OF CONSUMERS AND HEALTH PROFESSIONALS FROM THE QUESTIONS RECEIVED BY A DRUG INFORMATION CENTER GISELE DA SILVA BELCHIOR; ALICE GONÇALVES DE SOUZA; MIKAELLY PEREIRA CAET; DANIELLE MARTINS VENTURA; JULIANA GIVISIÉZ VALENTE; FERNANDA LACERDA DA SILVA MACHADO. Universidade Federal do Rio de Janeiro - Campus Macaé, Macaé - RJ - Brazil. Introduction and objective: One of the twelve core interventions to promote the rational use of medicines of the World Health Organization is to provide independent information on medicines. In this context, Drug Information Centers (DIC) remain highly relevant to provide unbiased, updated, evidence-based information in response to requests. The evaluation of the questions received by these services can help to comprehend the information needs of different groups. This work aims to describe the questions received by a DIC, comparing the interests of health professionals and consumers. Materials and methods: The questions were sent through an internet page from January 2014 to June 2017 and then were registered using a form developed in EpiInfo™. The requests were categorized according to the type of the question and the drugs involved according to the Anatomical Therapeutic Chemical (ATC) classification system. The data were analyzed using descriptive statistics. Results and conclusion: During this period, 178 questions were received, 59% sent by health professionals, mainly pharmacists (96%). The questions requested by consumers were chiefly related to drug interactions (30%) and involved drugs acting in Nervous system (32%). In contrast, health professionals predominantly asked about recommendations for use (17%), mostly comprising Antiinfectives for systemic use (25%). The results are relevant to defining permanent education programs for health professionals and to propose strategies tailored to inform specific groups of patients in order to meet their information needs. Financial support or fellowship: PROFAEX; PROEXT/MEC.

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CFSP406 E-LEARNING CONTINUING EDUCATION FOR PHARMACISTS IN PUBLIC HEALTH IN BRAZIL: A SWOT ANALYSIS FERNANDA MANZINI; NORBERTO RECH; SILVANA NAIR LEITE. Ufsc, Florianópolis - SC - Brazil. Introduction and objective: Between 2010 and 2015, 2,500 pharmacists who work in the Unified Health System (the Brazilian public health system – SUS) completed a specialization course on Pharmaceutical Assistance Management supported by the Ministry of Health and UFSC. The 15-month course was developed in an e-learning platform and four classroom meetings. All pharmacists had to develop an interventional project in their workplace at the end of the course. Aim: To evaluate the perspectives of nationwide politicians and course’s staff about the course development and its impact on the public pharmacy sector. Materials and methods: Through a nationwide workshop set up do evaluate the course (in 2016 and 2017), 68 stakeholders participated in a SWOT analysis (Strengths, Weaknesses, Opportunities, and Threats): a workshop with politicians and managers (representatives of Ministry of Health, State and Municipal governments), professional body representatives and course staff (tutors, regional coordinators, professors, supervisors) and another workshop with students. Results and conclusion: Four major subjects were identified: pedagogic project; course structure; learning tools; personal interaction process. The major strengths discussed were the gratuity, the decentralized infrastructure within the regions, technical support, practice in services as teaching methods, interventional projects and incentives for debates and forums. The major weaknesses were the huge amount of content, pharmacists and professors’ limited experience of e-learning, social differences within the geographic regions. This first step of the course evaluation concluded that it was a very important opportunity for pharmacists and public health services, and identified the pedagogic and structural issues to be improved in future courses. The results of the workshops will support the construction of a theoretical model and a matrix of indicators for the evaluation of the impact of continuing education for pharmacists in health services. Financial support or fellowship: Ministry of Health

HEALTH LITERACY OF PATIENTS WITH TYPE 2 DIABETES MELLITUS

CFSP407

HELLEN GENEROSO MOREIRA1; FÁBIO LUIZ MIALHE2; FÁTIMA HADDAD BARRACH1; MARCO ANTONIO VIEIRA DA SILVA3. Universidade Paulista (UNIP/Bauru), Bauru - SP - Brazil; 2Faculdade de Odontologia de Piracicaba (Fop/Unicamp), Piracicaba - SP - Brazil; 3Faculdade de Odontologia de Piracicaba e Universidade Paulista (UNIP/Bauru), Bauru - SP - Brazil. 1

Introduction and objective: Type 2 diabetes mellitus is considered a worldwide epidemic and, in this way, health services need to develop strategies for the health care of their patients. One of the methods used to identify the ability of people with diabetes to access, understand and use basic health information is called functional health literacy. Thus, the objective was to investigate health literacy levels in a group of diabetic patients. Materials and methods: The present study investigated the literacy levels of a group of 56 individuals with type 2 diabetes mellitus, using the instrument Brief Test of Functional Health Literacy (b-TOFHLA) and their relationships with the variables schooling, income and glycated hemoglobin test. The b-TOFHLA instrument was composed of 36 items of textual comprehension and 4 items of numerical comprehension, being translated and adapted culturally in Brazil in 2009. The analysis of glycated hemoglobin levels was performed using the high-performance liquid chromatography method, where levels greater than 7% were related to higher risks of chronic complications, and for this reason the goal of 7% was defined as the maximum acceptable limit in individuals with type 2 diabetes mellitus. Results and conclusion: The results showed that of the 56 (100%) individuals analyzed, 40 (71.43%) had inadequate levels of health literacy. And, of those who presented low levels of health literacy, the majority showed low income (95%) and low education (60%). Regarding the glycated hemoglobin test, in 32 individuals (57.14%) who presented results above 7%, there were insufficient health literacy levels in 22 individuals (68.75%). These data point to the importance of effective educational practices that develop new conceptions of the health-disease process and new health practices in patients with type 2 diabetes mellitus.

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CFSP408 INTERACTIONS BETWEEN TEACHING AND RESEARCH: DEVELOPMENT OF PHARMACEUTICAL SERVICES IN PRIMARY HEALTH CARE SAMARA JAMILE MENDES1; MYLLENA FARISCO1; SILVANA NAIR LEITE2; SILVIA STORPIRTIS1. Faculdade de Ciências Farmacêuticas USP Sp, São Paulo - SP - Brazil; 2Universidade Federal de Santa Catarina, Florianópolis - SC - Brazil. 1

Introduction and objective: In Brazil, from the innovation perspective of health professional education, considering the humanistic, critical, and reflexive approach in the Public Health Services (SUS), it is important to discuss the performance of these professionals in the health care process. This study analyzes interactions between teaching and research in pharmaceutical services (PS) in Primary Health Care (PHC) during a research project. Materials and methods: The focus group was used as a qualitative research method that seeks the dialogue of social actors and the discussion of significant themes. Its precepts are the subject’s participation to answer key questions through sharing ideas and experiences. Pharmacists of the Health Department of the Municipality of Sao Paulo were invited to participate in November 2016. The study was approved by the Research Ethics Committee of the Faculty of Pharmaceutical Sciences of Sao Paulo. Results and conclusion: Twenty pharmacists participated, of whom 18 (90%) work in Primary Health Care Units in the city of Sao Paulo; 11 (55%) have worked over 5 years in the SUS. It was possible to observe a strong interaction within the group, because all participants act in the PHC scenario. The themes that emerged were categorized as: pharmaceutical services, the understanding of their structure, importance, as well as the changing paradigm of pharmaceutical services from a product-based perspective to the needs of patients care. These aspects are advancing in Brazil, since pharmacists are improving their identity as a member of the health professional team. Universities should be an active partner in this process, and research applied to developing the services in the context of the SUS must be encouraged. Financial support or fellowship: Pró-Reitoria de Cultura e Extensão Universitária da USP; CNPq.

INTRINSIC MOTIVATION OF PHARMACY ACADEMICS IN THE ABP METHOD

CFSP409

MARINA MUNIZ AZEVEDO COSTA1; ELISANGELA CHRISTHIANNE BARBOSA DA SILVA GOMES2; JULIANY SILVEIRA BRAGLIA CÉSAR VIEIRA2. Faculdade Pernambucana de Saúde, Recife - Brazil; 2Faculdade Pernambucana de Saúde, Recife - PE - Brazil.

Introduction and objective: When motivated, individuals perform their activities better, since they do not need a greater stimulus. With this, the need arises to identify which are the main aspects that motivate students, so that they perform better in the graduation and this has good repercussions in their professional life. The Problem Based Learning method that has the student at the center has shown positive results, since it stimulates the curiosity and interest of the academics by its active methodology. Therefore, the objective of the study was to identify the motivational aspects of pharmacy academics in an active methodology. Materials and methods: Data were collected from a questionnaire with questions about the motivational aspects applied to pharmacy students from the 1st to the 9th semesters at an Institution of Higher Education,. Results and conclusion: It was observed that 62% of the students scored “interaction with classmates” as something motivating them in college, 62% “environment of tutoring”, 54% “subject of clinical cases”, 38% “empathy (Identification) with the tutor “, 35% “tutor performance” and 15% marked “other” which included internships and laboratory, demonstrating that practice and interaction are the motivational factors with the greatest impact on undergraduates.

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CFSP410 INVESTIGATION OF THE ANTINOCICEPTIVE POTENTIAL OF THE ETHANOLIC EXTRACTS OF THE LEAVES AND FLOWERS OF Tabebuia aurea SUELLEN MARIA ALBUQUERQUE DA SILVA; GERALDO JOSÉ DA SILVA NETO; EDLA KAROLINE CORDEIRO DE ALMEIDA; IVENIS RAPHAEL CAVALCANTE DO NASCIMENTO; MAX DENISSON MAURÍCIO VIANA; ELIANE APARECIDA CAMPESATTO. Universidade Federal de Alagoas, Maceió - AL - Brazil. Introduction and objective: The development of new analgesic drugs is of great interest because the drugs currently available on the market have limited efficacy and/or several side effects that restrict their use. Tabebuia aurea is a plant widely used in folk medicine as infusions for treatment of pain and inflammation, so this study aimed to investigate the antinociceptive potential of Tabebuia aurea ethanolic extracts (TAEE). Materials and methods: Two TAEEs were obtained, from leaves and flowers, using ethanol maceration extraction. Initially, cytotoxicity evaluation in vitro was made by colorimetric cell viability assay (methyltetrazole – MTT). After, Swiss mice (25-35g, n = 6), of both genres, were used for tests of abdominal writhing induced by acetic acid, and nociception induced by formalin. In all tests, TAEEs doses were 100 and 200 mg/Kg. Results and conclusion: In the MTT assay, none of the TAEEs doses showed damage to the cells. In the writhing test, all TAEEs doses were able to significantly reduce (p < 0.001) the number of writhes. In the formalin test, none of the TAEEs doses inhibited the pain response in the neurogenic phase, suggesting no involvement of the TAEEs action in the central pain modulation; however, all TAEEs doses were able to significantly reduce (p < 0.001) the licking time in the inflammatory phase. The results suggest that TAEEs do not present a central antinociceptive action but show a significant peripheral antinociceptive activity, contributing to the ethnopharmacological study of T. aurea. Financial support or fellowship: CPNq; UFAL.

MEDICATION USE AND CORRELATION WITH ADOLESCENT STUDENTS’ QUALITY OF LIFE

CFSP411

PAMELA MELLO VISSER; LUCIANA BERTOLDI NUCCI. Pontifícia Universidade Católica de Campinas, Campinas - SP - Brazil. Introduction and objective: Little is known about the quality of life and use of any kind of medication among school children. Quality of life is biopsychosocial wellbeing and can influence all areas of life. The main objective of this study was to evaluate the association of the use of any kind of medicine with the adolescent’s quality of life. Materials and methods: A cross sectional study was performed with 270 students aged 10-18 years in a public school, located in Jundiaí-SP. Of this group, 265 results were valid. Pediatric Quality of Life Inventory (PedsQL™ 4.0) Generic Core Scales were applied to students and a self-report questionnaire about the adolescent general health was sent to parents with the informed consent form. Quality of life was classified according to PedsQL 4.0 ™ instructions and the groups - 20 using any medicine and 245 not using - were compared. Results and conclusion: Total scores for quality of life was lower for those using any medicine comparing to the non-use group (p=0,012). The difference was also verified in the social domain, adolescents who use medicine have a lower quality of life in comparison to the group who do not use (p=0.010). We can conclude that the use of medicine may influence an important part of the adolescent’s life and is associated with their quality of life. More studies are suggested to better explain these differences.

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CFSP412 MULTIPROFESSIONAL HEALTH RESIDENCY AS A SPECIALIST PHARMACIST TRAINING MODEL: EXPERIENCE REPORT LIVIA MARIA GONCALVES BARBOSA; CARLOS HENRIQUE MOREIRA CUNHA; DÉBORA CECÍLIA MANTOVANI FAUSTINO DE CARVALHO; GRAZIELA GOMES BAUPTISTA MORENO. Hospital Sirio Libanes, Sao Paulo - SP - Brazil. Introduction and objective: The multiprofessional health residency is a postgraduate modality, latu sensu, characterized by inservice training, supervised by trained professionals (preceptors) and in a regime of exclusive dedication. The incorporation of the multiprofessional resident into care teams emerged in teaching hospitals focused on the integral patient assistance. The resident competencies required are: provide integral patient assistance, work as a member of a team, seek new opportunities and assume responsibilities with the patient and the health service. Currently, at Hospital Sírio Libanês there are four residency programs with vacancies for pharmacists. This work aims to describe the results of pharmacist training in the multiprofessional health residency. Materials and methods: Retrospective exploratory study using data from 2013 to 2017. The observed aspects were number of pharmacy residents trained in multiprofessional health programs and rate of residents absorbed by the hospital pharmacy service. Results and conclusion: The multiprofessional health programs at Hospital Sírio Libanês are: Critical Care - started in 2013, Oncology Care - started in 2014, Health Management - started in 2016 and Child and Adolescent Care - started in 2017. A total of 19 specialists were trained in the period, 11 (58%) in Critical Care, 08 (42%) in Oncology Care (Child and Adolescent Care and Health Management program are still in progress). Of those trained in the Oncology Care program, 06 (75%) are working in the trained area; in the Critical Care program, 01 (9%) works in the trained area. Of the total of 19 specialists, 07 (37%) were hired by Hospital Sírio Libanês (5 as effective members and 2 as temporary members), 07 (37%) were hired by other health institutions, 03 (16%) were employed in other related areas and 02 (10%) available in the market. The Brazilian regulation requires the specialization of the pharmacist to act in oncology, contributing for the insertion of this professional in the area of performance. The multiprofessional health residency emphasize the training in service and seems to be interesting for the professional and for the hospital. Financial support or fellowship: Hospital Sírio Libanes CFSP413 PHARMACEUTICAL ACTIVITIES IN THE MULTIPROFESSIONAL EMERGENCY RESIDENCY: THE FIRST YEAR OF TRAINING ANTONIA RAFAELLA FERREIRA GOMES MARTINS1; JOÃO PAULO DE MEDEIROS JÚNIOR2; BRENA GEYSE MESQUITA ROCHA SOARES3; ADNA VASCONCELOS FONTELES3; RAFAELA LINHARES PONTE RANGEL3. Santa Casa de Misericórdia de Sobral -Ce, Sobral - CE - Brazil; 2Instituto Superior de Teologia Aplicada, Cruz - CE - Brazil; Santa Casa de Misericórdia de Sobral-Ce, Sobral - CE - Brazil.

1 3

Introduction and objective: The purpose of the Multiprofessional Residency in Emergency (RMUE) of Santa Casa de Misericórdia de Sobral/CE (SCMS) is to develop highly trained health professionals who will work directly in this field. This field requires highly technical and scientific training of nurses, nutritionists, physiotherapists, and pharmacists, considering the importance of emergency care. The objective of this study was to describe the pharmacist’s role in clinical activities during the period of this in-service training model. Materials and methods: This is a descriptive study that occurred over a one-year period, from March 2016 to March 2017 at SCMS. The pharmacist initially identified the patient through hospital attendance records or attendance in the emergency room as part of the recognition process and evaluation of patients. The need to prepare an identification card for the patients who needed follow-up occurred in a systematic and documented way. Results and conclusion: Pharmacotherapeutical follow-up allowed for notification of drug-related problems. It was also observed through this monitoring that critical observations occurred in the sector’s health team, leading to changes in some configurations of the organizational and operational model. These changes raised their awareness of the co-responsibility for drug therapy, always with the idea of facilitating improvement to healthcare. A pharmaceutical resident learns to work in preparation for patient discharge through home care guidance. Through this experience, it is possible to verify the promotion of patient safety in pharmaceutical practice as the pharmacist should provide actions in the clinical pharmacy services to formulate and articulate processes to ensure effective care for the patient and how to prevent adverse events. More assertive training, not only limited to medicine, but also for a holistic view of patient care should be conducted after considering SUS principles. Financial support or fellowship: Santa Casa de Misericórdia de Sobral-CE/Instituto Superior de Teologia Aplicada

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CFSP414 PHARMACOTHERAPEUTIC FOLLOW-UP AS A LEARNING TOOL: A PEDAGOGICAL EXPERIENCE IN THE DISCIPLINE OF CLINICAL PHARMACY PRACTICES TÂNIA REGINA FERREIRA; KATIUSCA DA SILVA PONTES; CRISTIANE DE CÁSSIA BERGAMASCHI. Uniso, Sorocaba - SP - Brazil. Introduction and objective: Pharmacotherapeutic follow-up is defined as the practice in which the pharmacist is responsible for the patient’s needs related to the use of medicines. It is considered a tool used for Pharmaceutical Care which involves the detection, prevention and resolution of Problems Related to Medicines (PRM) in a systematic, continuous and documented way. The discipline of “Practice of Clinical Pharmacy” (80 hours workload), one of the curricular components professionalizing, is taught to 9th semester of the Pharmacy course of the University of Sorocaba-SP. Objective: To promote a Pharmacotherapeutic follow-up activity to stimulate and develop clinical reasoning in undergraduate pharmacy students in the discipline “Clinical Pharmacy Practices”. Materials and methods: The activity involved 16 students. The proposal of the activity was carried out after the lecture “Clinical methods for the practice of pharmaceutical care and clinical reasoning”. The students were responsible for choosing a patient and the most appropriate clinical method to be applied. The period determined for the activity was 3 months; the cases were discussed by the students and teacher in the classroom. The students were responsible for the reception, evaluation, determination of the therapeutic objectives, action planning, development of the planned actions and finally the underlying evaluations. At the end of the semester, clinical cases were presented for the assessment of a panel composed of the professor of the discipline and two clinical pharmacists. Results and conclusion: The students designed plans of action and intervention from the identified needs. Through counseling, health education practices and the implementation of follow-up therapeutic measures, it was possible to improve adherence to treatment and solve the PRM. Conclusion: The practical activity model promoted the understanding and expressive stimulation of the students about the importance of these skills for the clinical performance of the pharmacist.

PHARMINDER JR: A UNIVERSITY ENTREPRENEUR EXPERIENCE

CFSP415

DANIELA TODESCHINI1; NADYA LIE FATTORI2; ANA ROSA DE JESUS FUSCO3; GABRIELA PANKRATZ DE NOVAIS3; ÍSIS NALIN FERNANDES NONATO4; GIOVANNA MESSORE CARPINELLI MONTEIRO3; PAULO ROBERTO REGAZI MINARINI3; PATRICIA SANTOS LOPES3. Universidade Federal de São Paulo, Diadema - SP - Brazil; 2Universidade Federal de São Paulo, Suzano - SP - Brazil; Universidade Federal de São Paulo, São Paulo - SP - Brazil; 4Universidade Federal de São Paulo, Osasco - SP - Brazil.

1 3

Introduction and objective: The Junior Enterprise Movement (JEM) was created in France in 1967 with the aim of training committed professionals capable of transforming the environment in which they find themselves. This movement arrived in Brazil in 1988 to contribute to the development of an entrepreneurial country, so according to this, UNIFESP’s Junior Pharmacy Enterprise, Pharminder Jr, was created in 2014. This enterprise is a not-for-profit civil social organization, formed and managed exclusively by pharmacy undergraduate students, with the purpose of improving the education in a deficient area in the graduation: the business experience. Materials and methods: Pharminder Jr. brings a business environment, divided into seven executive boards: presidency, administrative and financial, marketing, quality assurance, project management, human resources and technical area. The organization focuses on the professional and academic development of students, the connection to the community through social projects and the provision of services to industries under the guidance of qualified professors. Results and conclusion: Nowadays, the Pharminder Jr. creation has beneficially impacted the UNIFESP - Campus Diadema, adding practical experience to theoretical skills and contributing to developing students` self-confidence and experiencing entrepreneurship at early stages, needed in today’s job market. The results, so far, showed that the development of projects for the academic community and companies indicate that it is possible to train a pharmacist with managerial and entrepreneurial skills, without losing their human essence.

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CFSP416 PROFILE OF UNDERGRADUATE DISSERTATIONS AND INTERNSHIPS IN A PHARMACY COURSE AT A PUBLIC UNIVERSITY IN SAO PAULO LAURA DE OLIVEIRA NASCIMENTO1; TAÍS FREIRE GALVÃO2; PAULO CÉSAR PIRES ROSA2; ROSIANE DE FÁTIMA LOPES AMBRÓSIO2; MARINA BORGES DIAS DE ALMEIDA2; PRISCILA GAVA MAZZOLA2. Unicamp, Campinas - Brazil; 2Unicamp, Campinas - SP - Brazil.

Introduction and objective: A typical pharmacy graduation course contains “fixed content” and “flexible content”. While the fixed content is pre-determined in each discipline, the flexible content can describe and reveal student preferences or environmental limitations for the final choice. Detailed knowledge of this content also aids in pedagogic plan redesigning. Our Pharmacy graduation course has flexible content such as free electives, internships and undergraduate dissertations. Therefore, we aimed to determine the profile of undergraduate dissertations and internships, according to the new proposal for the National Curriculum Guideline Pharmacy and market practices. Materials and methods: We collected and analyzed data from students that concluded their dissertations and internships between 2012 and 2016 from Unicamp’s Pharmacy course (Campinas-SP, Brazil). Obligatory internships in pharmacies were excluded from the study. We classified dissertations and internships in three areas, according to the guideline: Technology and innovation, Pharmaceutical management, and Pharmaceutical care. We further calculated frequencies and agreement (unweighted kappa analysis) between internships and dissertation areas. We also classified internships according to professional areas and dissertations according to word frequency from themes to explore patterns. Results and conclusion: In total, 138 students concluded their undergraduate dissertations and internships between 2012-2016, with an average graduation time of 6 + 1 years (5 years minimum course time). Most internships (51% discipline 1 and 59% discipline 2) and dissertations (64%) were classified as Technology and Innovation. 43% of students concluded dissertation and both internships in the same field. There was low agreement between the internships and dissertations (Kappa = 0.2), but higher agreement between both internships (kappa = 0.6). The relevance of technology and innovation showed in the profile reflected a high number of opportunities in scientific initiation in our University (used for experimental based dissertations) and high industrial internship spots from the local market. Word frequency showed that evaluation, treatment and characterization were the most frequent goals in dissertation themes, whereas “cancer” was the most frequent disease studied. Considering 15 professional areas, quality control was the most frequent for both internships (21-27%). In conclusion, we described a detailed profile of undergraduate dissertations and internships, bringing relevant information for further pedagogical and administrative course decisions. Financial support or fellowship: Unicamp CFSP417 RELEVANCE OF A RESIDENCY PROGRAM FOR SPECIALIST PHARMACIST TRAINING: PERCEPTIONS OF PHARMACY RESIDENTS OF A MULTIPROFESSIONAL CRITICAL CARE PROGRAM LIVIA MARIA GONCALVES BARBOSA; RAFAELA PASCOLI CAMARGO; BIANCA AUGUSTA PEREIRA DE PAULA; SHEILA BORGES ROCHA BARROS FERREIRA; SARA BATISTA NOGUEIRA. Hospital Sirio Libanes, Sao Paulo - SP - Brazil. Introduction and objective: The Multiprofessional Residency in Critical Care is a lato sensu specialization characterized by a workload of 60 hours per week, of which 80% are dedicated to practical activities in a multiprofessional team with emphasis on critically ill patient care. The aim of this study is to present the experiences and perceptions of pharmacy residents in a large philanthropic hospital. Materials and methods: Descriptive study based on the critical reflexive analysis of the first year experiences of four pharmacists of a multiprofessional residency program focused on critical care, considering activities developed in practical and theoretical areas. Results and conclusion: Concerning the practical activities, the residents rotate through different units of the hospital and in units of the Brazilian Unified Health System (SUS) of different levels of complexity, acting directly with the multiprofessional team to assist different patient profiles. This experience allows the development of a clinical view, critical thinking and identification of opportunities for improvement in patient care. In the theoretical scope, the activities are focused on the use of active teaching-learning methodologies. These consist in meetings for analysis and discussion of everyday problem-situations, exposed in tutorial sessions, realistic simulations and portfolios. The active methodology emphasizes the importance of identifying learning needs based on experiences in clinical practice, which allows the consolidation of knowledge. The processes experienced during residency were fundamental for the improvement of technical knowledge, development of behavioral skills and strengthening of professional identity. Financial support or fellowship: Hospital Sirio Libanes

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CFSP418 THE LEARNING PROCESS IN EAD AND PHARMACEUTICAL PERCEPTION IN TRAINING ON PHARMACEUTICAL ASSISTANCE MANAGEMENT KATIUCE ALVES DE CASTRO1; MARENI ROCHA FARIAS1; SILVANA NAIR LEITE1; ADALBERTO ALVES DE CASTRO2. Universidade Federal de Santa Cataria - Ufsc, Florianópolis - SC - Brazil; 2Centro Universitário Barriga Verde - Unibave, Orleans - SC - Brazil. 1

Introduction and objective: The growth of public investment in pharmaceutical care has required increasing quality of pharmaceutical services. The inclusion of new information and communication technologies (ICTs) associated with the need for permanent health education enables Distance Education (EaD), which manifests itself as a professional alternative. The flexibility of distance learning aids in the learning process in contextualized and decentralized education. The study purpose was to present the Pharmacist profile whose source for training for the Pharmaceutical Care Management and investigate their perception of their learning process in Pharmaceutical Care Management in the second edition of the Postgraduate distance course. Materials and methods: This is descriptive longitudinal research, with a quantitative approach. The present study is approved, certificate number 1.231.402/2015 in Plataforma Brasil. Pharmacist population enrolled in the 2nd edition of the Pharmaceutical Care Management Course - EaD, offered 1,600 vacancies for the UFSC. Results and conclusion: A total of 1,453 (95.4%) Pharmacists were from Unified Health System (SUS) and 70 (4.6%) were teachers on undergraduate courses. An association was found between the course level and the final approval of the student, and enrollment in Specialization, student participants located in the Southeast, those who had already participated in a Distance Education course and those who had good internet connection were more likely to pass the Course. In addition, students aged between 35 and 39 years and those that had more than three jobs had lower probability of passing. Over 90% of students in all regions evaluated the contents and teaching strategies adopted for their process of learning and applying to their working reality positively. Pharmacists who sought training in the second edition of the Pharmaceutical Care Management Course -EaD face several conditions of work and barriers to meeting the lifelong learning and evaluate positively highly the process of learning and application in professional reality of the Management in Pharmaceutical Care Distance Education Course. Financial support or fellowship: CAPES

THE PHARMACY COURSE APPROACH AT THE FEDERAL PROJECT PET GRADUA-SUS

CFSP419

RHYE LESSA ISHIKAWA1; DANIELI SIBIONI1; DANILO FIDELIS1; ALTAIR GASPARETTO2; FELIPE ASSAN REMONDI3; LILIAN TANIDA4; ROGER BRUNO RODRIGUES2; TIAGO FERREIRA2; VALNIZE LAMBERTI5; AIRTON JOSÉ PETRIS1; GRAZIELA SCALIANTI CERAVOLO1; SANDRA REGINA CARVALHO1; EDMARLON GIROTTO1. Universidade Estadual de Londrina, Londrina - PR - Brazil; 2Municipal Health Department of Londrina, Londrina - PR - Brazil; 17th Regional Health Division in the State of Paraná, Londrina - PR - Brazil; 4Municipal Health Department of Ibiporã, Ibiporã PR - Brazil; 5Municipal Health Department of Cambé, Cambé - PR - Brazil. 1 3

Introduction and objective: The PET-Saúde GraduaSUS Project is a federal program that aims to promote the integration educationservice-community of graduation courses in the health field, instigate national curricular changes, articulate actions that promote the approach to Unified Health System, continued education and better service to patients. Materials and methods: The project has been developed by graduation courses at the State University of Londrina (UEL): Medicine, Pharmacy, Nursing, Dentistry, Physiotherapy, Veterinary Medicine and Biology. Each work team is formed of undergraduates, tutors and preceptors of the Unified Health System. During the first year of the project, workshops were held and initiatives were planned to fill knowledge gaps suggested by the public service network of the cities. Results and conclusion: Initially, training was organized regarding Sources of Information about Medications along with pharmacists from the Unified Health System and students of the Pharmacy course. Moreover, an agreement was signed between State University of Londrina and Municipal Health Department of Londrina, enabling dispensing of the city’s essential medications through the “Pharmacy School”. Also, a discussion about improvement to the Pharmacy course curriculum in what concerns the pharmacists’ performance at Unified Health Systems was conducted with the actors involved in the Project. Overall, the project brought the Pharmacy course closer to the public health system, which will possibly contribute to a more adequate preparation for the student’s practice along with it and establish a communication channel between university and service. Financial support or fellowship: National Health Fund – Brazilian Ministry of Health

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CFSP420 THE UNIVERSITY AND THE DEVELOPMENT OF STRATEGIES IN PREVENTION OF THE EXPANSION OF DENGUE FEVER IN A REGIONAL CONTEXT ELIANA ANUNCIATO FRANCO DE CAMARGO; DENISE VALLIM PASOTTI; JOSÉ TARCÍSIO FRANCO DE CAMARGO; ADEMIR SALVI JÚNIOR. Centro Regional Universitário de Espírito Santo do Pinhal - UNIPinhal, Espirito Santo do Pinhal - SP - Brazil. Introduction and objective: The university is a privileged space that encourages the exchange and aggregation of heterogeneous knowledge, indispensable to the formation of future professionals. Aiming to express the university’s social commitment through research actions, the entire academic community of undergraduate courses in Pharmacy, Biomedicine, Gastronomy, Nursing, Mechatronics Engineering, Physical Education and Computer Engineering of UniPinhal was mobilized in the massive dissemination of information and the destruction of breeding sites of the mosquito transmitter of dengue fever in the municipality of Espírito Santo do Pinhal-SP. Materials and methods: The municipality was subdivided into sectors and weekly, during the months of March and April, a representative of the Zoonoses Control Center, community agents, City Hall volunteers and UniPinhal students and teachers organized into teams - took turns at home visits in the process of active search and destruction of breeding sites of the Aedes aegypti mosquito. Results and conclusion: During the month of March, 6,419 buildings were visited and 71.5% of the owners approached allowed the surveying teams entrance. In April, 6,088 buildings were visited and 71.7% of them were accessible for survey. The weekly average was of 1,563 sites visited - where countless breeding sites of the mosquito were destroyed. This way, actions such as these strengthen the social role of the University as a promoter of actions focused on the welfare of the population and the exercise of citizenship. Financial support or fellowship: Centro Regional Universitário de Espírito de Pinhal - UniPinhal CFSP421 USE OF MEDICINAL PLANTS, LUDIC APPROACH AS A PEDAGOGICAL TOOL IN TEACHING TOXICOLOGY JOAQUIM HORÁCIO DE ARAÚJO NETO1; DANILO ANDRADE DE ARAGÃO1; GUILHERME MENDES PRADO1; GRACIANNY FERNANDES MAGALHÃES1; MÁRCIA JORDANA ARAÚJO2; WILCARE DE MEDEIROS CORDEIRO NASCIMENTO1; RAFAELLY MARIA PINHEIRO SIQUEIRA1. Instituto Superior de Teologia Aplicada (Inta), Sobral - CE - Brazil; 2Instituto Superior de Teologia Aplicada (Inta), Sobral - Brazil.

Introduction and objective: From the beginning, plants have been used for various therapeutic purposes, one of the earliest descriptions of the subject was made on the Ébers Papyrus. The scarce amount of printed studies on medicinal plants and the false ideology that “the natural does no harm” worries health professionals given the high, often irrational, consumption of these plant and/or herbal drugs and the potential for intoxication. Aiming to solve this problem, a board and card game was developed, a pedagogical tool for Academics of the Pharmacy course, addressing the main toxic actions of some medicinal plants listed in the literature as the most consumed by the Brazilian population. We aim to contribute knowledge about the rational use of medicinal plants and to consolidate this knowledge related to toxicity through a playful learning tool with the capacity to evaluate the student’s knowledge in this type of intoxication. Materials and methods: The game was discussed with academics of the 5th semester of the Pharmacy course during a class of General Toxicology at the Instituto Superior de Teologia Aplicada (INTA), located in the city of Sobral, Ceará, Brazil. The content of the game is based on information extracted from the literature of Dr. Francisco José de Abreu Matos, creator of the Farmácia viva project by the Universidade Federal do Ceará (UFC). Results and conclusion: The students had already taken the Pharmacognosy course and were studying General Toxicology with the content of Toxic Plants already completed before the moment of application of the game. We observed that 70% of a class of 30 students reached a satisfactory percentage of correct answers. It can be concluded that the game presents a great contribution to the learning of the students, represents a playful tool that, through competition, instigates knowledge about the subject and the reflection on the risks of irrational use of these medicinal plants. Financial support or fellowship: INTA

Brazilian Journal of Pharmaceutical Sciences vol. 53, suppl. 3, 2017

USE OF PHOTOS: A REINFORCED WAY TO RECOGNIZE BLOOD CELLS

231

CFSP422

ALCÍNIA BRAGA DE LIMA ARRUDA1; NATALHA NAYANE DE OLIVEIRA PINHEIRO1; ATHILA WESLEY LIMA LACERDA1; SANDNA LARISSA FREITAS DOS SANTOS1; MARIANA ALBUQUERQUE DE ARAÚJO1; JÉSSICA BEZERRA DA COSTA1; AMANDA APARECIDA DE LIMA ARRUDA1; ANIO IVAN HOLANDA LIMA2. Universidade Federal do Ceará, Fortaleza - CE - Brazil; 2Universidade de Fortaleza, Fortaleza - CE - Brazil.

Introduction and objective: The Clinical Hematology course included in the Pharmacy syllabus at Universidade Federal do Ceará is composed of theoretical and practical classes. Students identify, analyze and interpret blood smear slides using optical microscopy in practical classes. Furthermore, they are using a new methodology with photos of the slides studied in the practical classes, in order to identify, characterize and classify the cells, as well as to describe in which situations they occurred. Objective: Analyzing this new methodology used in the discipline and evaluating the knowledge of the Pharmacy students about morphology of blood cells. Materials and methods: The work was performed in three stages: first, the blood smear slides were photographed, edited and sent to students. The students analyzed the cells and answered a questionnaire about these photos. Lastly, the professor and the Teacher Assistant received the answers given by each student to quantify and qualify them. Results and conclusion: Results showed that 55% of the students responded to the questionnaire. It was observed that 83.63% and 97.72% of the students easily recognized mature leukocytes and erythroblasts, respectively, and they were able to relate them to some physiological or pathological situation. 82.72% were able to identify erythrocyte alterations and associate them with the corresponding anemias. Regarding viral infections, 90.9% of the students had difficulty identifying atypical lymphocytes, confusing them with monocytes. Regarding bacterial infections, 95.5% did not observe toxic granulations in neutrophils, confusing them with eosinophils. E, 86.35% of the students had difficulty identifying leukemias, as well as associating the young cell with leukemia equivalent. It is concluded that this methodology can be an additional tool for the study of hematology, since it has helped teachers and their assistants to detect student failures, which need to be worked on and solidified, improving students’ learning. Financial support or fellowship: UFC CFSP423 USP UNIVERSITY PHARMACY EXPERIENCE IN TEACHING-SERVICE INTEGRATION: A PARTNERSHIP WITH MUNICIPAL HEALTH DEPARTMENT OF SAO PAULO ON PHARMACOTHERAPEUTIC FOLLOW UP SAMARA JAMILE MENDES; PATRICIA MELO AGUIAR; TÁCIO DE MENDONÇA LIMA; MARIA GABRIELA BORRACHA; RENATA CUNHA CARVALHO; MAGALI DA SILVA PACHECO NOBRE ROSSI; MARIA GORETTI FARIAS DE LIMA; ANA LUIZA PEREIRA MOREIRA MORI; MARIA APARECIDA NICOLETTI; SILVIA STORPIRTIS. Faculdade de Ciências Farmacêuticas USP Sp, São Paulo - SP - Brazil. Introduction and objective: The USP University Pharmacy (FARMUSP) develops clinical practice activities in the areas of teaching, research and outreach activities focused on the community. From the need to qualify the pharmacists who work at the Health Units of the Municipal Health Department of Sao Paulo, a teaching-service partnership was established among these institutions to offer a course in pharmacotherapeutic follow-up of chronic patients. The aim of the present study was to describe the experience of implementing this course offered by FARMUSP. Materials and methods: The course had two editions in 2016, with 60 places for each edition. The approach was the pharmacotherapeutic follow-up service (initial assessment, care plan design and results evaluation) and, as a final activity, pharmacists worked out a project to implement pharmacotherapeutic follow up in their workplace. The reflection on the necessary strategies was mediated by SWOT (strengths, weaknesses, opportunities and threats) analysis. When the course was finished, the pharmacists performed a content seizure test and answered a satisfaction questionnaire. Results and conclusion: Of the 120 pharmacists who started the course, 111 completed it. More than half of the pharmacists scored 80 in the final test. Forty-two percent of pharmacists work in Health Units with Family Health Strategy and 91% stated that the Unit has only one pharmacist. Regarding content satisfaction, forty six rated it as very good. Forty three said that the material provided was very good. The course workload, in hours, was considered good by 36% of the students, however there were suggestions to increase it. Organizers hoped that this course would contribute to the effective insertion of the pharmaceutical services in the daily life of the Health Units of Sao Paulo.

Brazilian Journal of Pharmaceutical Sciences

AUTHOR INDEX/ÍNDICE DE AUTORES

A ABRANTES, F. A. ABREU, A. P. A. ABREU, I. C. ACCARINI, G. B. AGUIAR, A. C. M.

CFSP290 CFSP032, CFSP118 CFSP256 CFSP111 CFSP062, CFSP189, CFSP214 AGUIAR, A. M. CFSP062, CFSP189, CFSP214 AGUIAR, E. E. G. CFSP332 AGUIAR, P. M. CFSP423 ALBUQUERQUE, M. A. CFSP150, CFSP155 ALBUQUERQUE, S. CFSP257 ALBUQUERQUE, T. C. CFSP304 ALBUQUERQUE, V. O. CFSP148 ALCOFORADO, R. S. R. CFSP085 ALENCAR, G. O. CFSP300, CFSP353, CFSP368 ALENCAR, J. P. O. CFSP300 ALIBERTI, M. J. R. CFSP156 ALMEIDA, A. D. CFSP078 ALMEIDA, A. H. CFSP361 ALMEIDA, B. V. CFSP011 ALMEIDA, E. K. C. CFSP410 ALMEIDA, M. B. D. CFSP416 ALMEIDA, M. B. CFSP239 ALMEIDA, M. Q. CFSP103 ALMEIDA, M. S. J. CFSP324 ALMEIDA, P. H. R. F. CFSP058, CFSP107 ALMEIDA, R. L. CFSP003 ALMEIDA, S. C. CFSP254 ALMEIDA, S. S. M. S. CFSP305, CFSP306 ALMEIDA, T. S. CFSP187 ALMEIDA, V. A. CFSP057 ALMEIDA, V. L. C. CFSP144 ALMODOVAR, A. A. B. CFSP278, CFSP337 ALVARENGA, J. F. R. CFSP390 ÁLVARES, R. F. A. CFSP324 ALVES, A. É. G. CFSP298, CFSP333 ALVES, D. T. V. CFSP070, CFSP079, CFSP085, CFSP088, CFSP121, CFSP164, CFSP277, CFSP302 ALVES, H. H. S. CFSP157 ALVES, J. D. CFSP349 ALVES, M. M. CFSP036 AMARAL, A. CFSP363 AMARAL, C. M. O. CFSP271 AMARAL, E. A. G. CFSP197 AMARAL, H. E. G. CFSP202

AMARO, A. R. B. AMBRÓSIO, R. F. L. AMÉRICO, M. F. AMORIM, A. L. AMORIM, M. E. S. AMORISO, P. M. ANDRADE, M. A. ANDRADE, R. G. ANDRÉO FILHO, N. ANDRIOCIOLI, V. N. ANECHINI, L. D. V. ANGOLINI, C. F. F. ANJOS, L. N. A. ANTONIALLI, M. M. S. ANTONIO, J. M. ANTUNES, E. A. B. ANTUNES, V. M. S.

CFSP162 CFSP416 CFSP275 CFSP306, CFSP305 CFSP321, CFSP356 CFSP190 CFSP027, CFSP081 CFSP047 CFSP263 CFSP211 CFSP096 CFSP288, CFSP330 CFSP261 CFSP206 CFSP117 CFSP271 CFSP097, CFSP212, CFSP402 ARAGÃO, D. A. CFSP421 ARANTES, T. CFSP100 ARAÚJO NETO, J. H. CFSP051, CFSP421 ARAUJO, A. P. V. CFSP109, CFSP138, CFSP140, CFSP398 ARAUJO, B. T. R. CFSP161, CFSP225 ARAÚJO, G. S. CFSP173 ARAUJO, J. T. C. CFSP227 ARAUJO, L. M. CFSP137 ARAÚJO, M. A. CFSP422 ARAÚJO, M. J. CFSP051, CFSP421 ARCHONDO, M. E. D. L. CFSP106, CFSP314 ARMANDO JR., J. CFSP252, CFSP292 ARMESTO, C. CFSP386 ARNAUD, F. H. L. O. CFSP005, CFSP237 ARRUDA, A. A. L. CFSP422 ARRUDA, A. B. L. CFSP422 ARRUDA, G. L. M. CFSP288, CFSP330 ASANOME, D. Z. CFSP083 ASSIS, J. R. CFSP029, CFSP137, CFSP201 AVELINO, M. M. CFSP389 ÁVILA, R. A. M. CFSP370 AVILES, J. CFSP362 AZEVEDO FILHO, C. A. CFSP222, CFSP297 AZEVEDO, D. Q. CFSP104 AZEVEDO, E. P. P. CFSP222 B BACELAR, T. S. BACH, E. E. BACHIR, H.

CFSP152 CFSP200 CFSP231

Brazilian Journal of Pharmaceutical Sciences

AUTHOR INDEX/ÍNDICE DE AUTORES

BANIN, J. P. V. BARACAT, M. M. BARBOSA, C. R. BARBOSA, F. W. X. BARBOSA, F. BARBOSA, J. Í. S. BARBOSA, L. M. G. BARBOSA, M. B. R. BARBOSA, R. A. G. BARRACH, F. H.

BARRETO, A. BARRETO, M. F. R. BARRETO, P. A. R. BARRETO, R. A. P. BARRETTO, Í. K. S. P. BARROS, C. T. BARROS, F. M. B. BARROS, K. B. N. T. BARROS, T. C. BASSI, A. A. C. BASTOS, J. K. BASTOS, S. C. BATALINI, C. BATISTA, F. S. BATTISTELLA, A. C. BAZO, A. P. BECKMANN, A. P. S. BELCHIOR, G. S. BELIZARIO, G. S. BELL, M. J. V. BELMONTE, C. L. BENTO FILHO, R. F. BENTO, A. G. BENVENUTTI, R. C. BERALDO-DE-ARAUJO, A. BERALDO-DE-ARAUJO, V. L. BERGAMASCHI, C. C.

BERNARDO, L. R. BERTASSOLI, B. M. BERTOLINO, R. A. BERTOLOTO, M. I.

CFSP035 CFSP228, CFSP267 CFSP028 CFSP186, CFSP191, CFSP331 CFSP213 CFSP301 CFSP412, CFSP417 CFSP006, CFSP383, CFSP384 CFSP142 CFSP086, CFSP365, CFSP367, CFSP371, CFSP407 CFSP160 CFSP300, CFSP353, CFSP368 CFSP035 CFSP248 CFSP011 CFSP021, CFSP089, CFSP139 CFSP280 CFSP157 CFSP367 CFSP066, CFSP094 CFSP257 CFSP144 CFSP232 CFSP160 CFSP067, CFSP149 CFSP049, CFSP203 CFSP355 CFSP405 CFSP069 CFSP226 CFSP130, CFSP231 CFSP160 CFSP322 CFSP040, CFSP067, CFSP149, CFSP153 CFSP221 CFSP221 CFSP021, CFSP089, CFSP098, CFSP139, CFSP414 CFSP323 CFSP361 CFSP402 CFSP146

BERTOLUCI, S. R. O. BESERRA, L. V. S. BESERRA, M. C. S. R. BETTINI, V. S. BEZERRA, C. A. BEZERRA, E. R. BEZERRA, J. S. BEZERRA, L. O. BEZERRA, S. B. BEZERRA, S. R. A. BIANCHI, C. BIASI-GARBIN, R. P. BIASOLI, M. R. BISPO, A. C. C. BISSOLI, L. R. BOAVA, A. C. BOCCHIGLIERI, M. M. BOFF, E. BOIAGO, I. A. BOLPETTI, A. P. BONETTI, F. M. R. BONIFÁCIO, A. G. BORDAN, B. D. BORGES, B. M. S. BORGES, K. D. M. BORGES, L. J. G. C. BORGHI, M. BORINI, G. B. BORRACHA, M. G. BOSA, L. M. BOTONO, K. M. BRAGA, A. V. BRAGA, A. BRAGA, M. R. P. BRAGA, M. S. BRANDÃO, M. C. N. P. BRANDÃO, R. M. A. BRASILEIRO, M. A. A. BRAVO, T. R. P. BREITKREITZ, M. C. BRIGANTI, C. P. BRITO, G. C. BRITO, G. E. G. BRITO, T. F. BRITO, V. O. C.

CFSP220, CFSP308 CFSP381 CFSP031 CFSP279, CFSP296 CFSP300, CFSP353, CFSP368 CFSP248 CFSP108 CFSP350 CFSP152, CFSP260, CFSP273 CFSP177, CFSP183 CFSP314 CFSP217 CFSP144 CFSP340 CFSP003, CFSP250 CFSP014, CFSP078, CFSP105, CFSP111 CFSP385, CFSP401 CFSP040, CFSP149, CFSP067, CFSP153 CFSP096 CFSP102 CFSP287 CFSP020 CFSP075 CFSP005, CFSP237 CFSP353 CFSP254 CFSP403 CFSP244, CFSP332 CFSP041, CFSP179, CFSP423 CFSP370 CFSP016, CFSP069 CFSP320 CFSP363 CFSP108 CFSP271 CFSP226 CFSP137 CFSP259, CFSP316 CFSP327 CFSP223, CFSP294 CFSP021 CFSP404 CFSP181, CFSP205 CFSP128 CFSP008

Brazilian Journal of Pharmaceutical Sciences

AUTHOR INDEX/ÍNDICE DE AUTORES

BRUNH, F. H. P. BUENO, C. T. BUGNO, A. C CABRAL, A. G. S. CABRAL, Y. M. S. CAET, M. P. CAETANO, C. R. CAETANO, J. L. CAJADO-CARVALHO, D. CALEFI, P. S. CALLADO, G. T. CAMARGO, E. A. F. CAMARGO, J. T. F. CAMARGO, P. R. C. CAMARGO, P. CAMARGO, R. P. CAMILO, T. G. N. CAMINADA, S. M. L. CAMPESATTO, E. A. CAMPOS E. V. CAMPOS, B. M. CAMPOS, C. M. D. CAMPOS, P. M. CANDIDO, E. P. CANIBAL, N. A. CAPERUTO, E. C. CARASKI, L. CARDOSO, C. A. L. CARDOSO, C. S. CARDOSO, F. C. S. CARDOSO, G. S. CARDOSO, M. H. M. CARDOSO, N. A. CARDOSO, R. R. CARDOSO, R. S. CARDOSO, V. N. CARLOS, E. R. C. CARMO, P. L.

CFSP223 CFSP124 CFSP337

CFSP318, CFSP351 CFSP310, CFSP329 CFSP405 CFSP035 CFSP260 CFSP295 CFSP257 CFSP376 CFSP194, CFSP420 CFSP194, CFSP420 CFSP291 CFSP086 CFSP417 CFSP327 CFSP385, CFSP401 CFSP410 CFSP204, CFSP209 CFSP200, CFSP254 CFSP155 CFSP286 CFSP376 CFSP151 CFSP026 CFSP376 CFSP268 CFSP206 CFSP027, CFSP081 CFSP255 CFSP025 CFSP147 CFSP275 CFSP107 CFSP290 CFSP031 CFSP341, CFSP345, CFSP346 CARMONA, M. J. C. CFSP142 CARNEIRO, A. M. F. CFSP182 CARNEIRO, F. M. CFSP144 CARNEIRO, M. S. CFSP065 CARNEIRO, R. M. CFSP347 CARNEIRO, T. O. CFSP404 CARNEVALE, R. C. CFSP096, CFSP101 CARVALHO SOBRINHO, J. R. B. CFSP399 CARVALHO, A. A. CFSP404

CARVALHO, A. F. CARVALHO, A. K. CARVALHO, D. C. M. F. CARVALHO, F. C. CARVALHO, F. S. CARVALHO, G. C. CARVALHO, J. B. R. CARVALHO, M. F. C. CARVALHO, M. R. CARVALHO, N. C. C. CARVALHO, P. O. CARVALHO, R. C. CARVALHO, R. L. CARVALHO, R. S. H. CARVALHO, S. O. CARVALHO, S. R.

CFSP326 CFSP026 CFSP412 CFSP312 CFSP301 CFSP271 CFSP351 CFSP073 CFSP303 CFSP311 CFSP288, CFSP330 CFSP423 CFSP010, CFSP015 CFSP314 CFSP101 CFSP228, CFSP267, CFSP419 CASAGRANDE, R. CFSP228, CFSP267 CASAVELHA, A. S. CFSP264 CASSAMASSIMO, R. P. CFSP207 CASTILHO, A. C. S. CFSP335 CASTILHO, J. C. CFSP151 CASTILHO, R. O. CFSP290 CASTRO JR., J. R. CFSP311 CASTRO, A. A. CFSP172, CFSP190, CFSP208, CFSP418 CASTRO, K. A. CFSP418 CASTRO, P. F. CFSP083 CASTRO, S. R. CFSP233, CFSP283, CFSP294 CASTRO, Y. T. CFSP397 CAVALARO, V. CFSP097 CAVALCANTE, M. R. CFSP052 CAVALCANTE, O. S. S. CFSP012 CAVALCANTE, R. C. M. CFSP404 CAVALCANTI, J. M. CFSP012 CAVALINI, F. CFSP200 CERAVOLO, G. S. CFSP419 CERDA, A. CFSP362, CFSP363, CFSP369 CESAR, A. L. A. CFSP243, CFSP290 CÉSAR, I. C. CFSP242 CESARETT, M. L. R. CFSP064 CHAIA, A. A. CFSP400 CHARELLI, G. A. CFSP326 CHAVES, O. F. M. CFSP033 CHÁVEZ-OLORTEGUI, C. CFSP370 CIBELLA, N. Z. CFSP390, CFSP397 CIPRIANO, S. L. CFSP120, CFSP132 CIUFFI, K. J. CFSP257

Brazilian Journal of Pharmaceutical Sciences

AUTHOR INDEX/ÍNDICE DE AUTORES

CLEMENTE, M. S. CLÍMACO, E. C. COELHO, M. M. COIMBRA, C. G. O. COMARELLA, L. CONCEIÇÃO, V. A. CONSTANTINO, A. T. CORADI, A. E. P.

CFSP234 CFSP071 CFSP320 CFSP218, CFSP219 CFSP383 CFSP029 CFSP223 CFSP116, CFSP126, CFSP173, CFSP192 CORDEIRO, C. S. CFSP059 CORDEIRO, R. P. CFSP321, CFSP343, CFSP356, CFSP380 CORRALO, V. S. CFSP040, CFSP067, CFSP149, CFSP153 CORREA, A. A. F. CFSP352, CFSP365, CFSP371 CORREA, C. R. CFSP211 CORRÊA, D. C. M. CFSP350 CORREA, O. M. T. CFSP252, CFSP361 CORRÊA, P. B. R. CFSP019, CFSP044 CORRÊA, T. W. CFSP324 CORREIA, R. D. CFSP029 CFSP201 COSTA JR., V. L. CFSP016, CFSP036, CFSP069 COSTA, C. S. CFSP049, CFSP172, CFSP190 COSTA, D. G. CFSP318 COSTA, D. G. CFSP321 COSTA, E. L. CFSP225 COSTA, F. A. CFSP048 COSTA, F. S. CFSP160 COSTA, G. A. CFSP340 COSTA, J. B. CFSP394, CFSP422 COSTA, J. R. M. CFSP301 COSTA, J. S. R. CFSP221, CFSP293 COSTA, L. M. M. CFSP001, CFSP037 COSTA, M. M. A. CFSP222 COSTA, M. M. A. CFSP409 COSTA, S. O. A. M. CFSP320 COTRIM, P. C. CFSP293 COUTO, R. O. CFSP171 CREDIDIO, L. CFSP288 CROVADOR, C. S. CFSP001, CFSP037 CROZARA, M. A. CFSP016 CRUZ, F. C. CFSP216 CRUZ, L. A. CFSP136 CRUZ, M. J. F. CFSP260 CRUZ, N. L. M. CFSP109, CFSP138, CFSP140, CFSP398 CUNHA N. L. CFSP257, CFSP262

CUNHA, C. C. CFSP256 CUNHA, C. E. P. CFSP319 CUNHA, C. H. M. CFSP412 CUNHA, W. R. CFSP257, CFSP262 CUNICO, C. CFSP035 CURBETE, M. M. CFSP065, CFSP125, CFSP185 CUSTODIO, A. S. CFSP116, CFSP192 CUSTODIO, J. L. CFSP116, CFSP192 D DABBUR, F. S. DAMASCENO, A. M. S. DAMASCENO, B. P. G. L. DAMASCENO, L. T. A. DARRIEUX, F. C. C. DAVID, F. L. DEL FIOL, F. S. DELUQUI, T. C. DESTRO, F. A. DETONI, L. S. DEWULF, N. L. S. DIAS, B. B. DIAS, Í. S. S. DIAS, J. B. DIAS, L. P. DINI, M. M. J. DINIZ, B. S. C. DIONATO, D. V. DOLABELA, M. F. DOREA, E. L. DOURADO, D. DUARTE, F. C. DUARTE, J. C. DUARTE, N. C. DUQUE, M. D. DURANTE, G. P. DUREGGER, K. DUZZI, B. E ELIAS, B. L. ELLER, E. E. ERICEIRA, A. J. C. ESGOTTI, M. B. ESPINAR, F. J. O. ESTRELA, H. F. G. EVA SOBRINHO, M. EVANGELISTA, C. R. S.

CFSP261, CFSP301 CFSP364 CFSP236, CFSP240 CFSP052, CFSP331 CFSP124 CFSP389 CFSP059 CFSP389 CFSP086 CFSP135 CFSP068 CFSP050 CFSP181, CFSP205 CFSP376 CFSP028 CFSP366 CFSP319 CFSP193, CFSP196 CFSP061, CFSP364 CFSP363 CFSP247 CFSP372, CFSP373 CFSP171 CFSP028, CFSP141 CFSP235 CFSP004 CFSP342 CFSP295

CFSP045 CFSP137 CFSP256 CFSP072 CFSP227 CFSP063, CFSP148, CFSP180 CFSP038 CFSP237

Brazilian Journal of Pharmaceutical Sciences

AUTHOR INDEX/ÍNDICE DE AUTORES

F FABIANO, A. R. FACIN, M. E. FAGUNDES, M. I. FAJARDO, C. FALCÃO, J. S. A. FARACO, A. A. G. FARHAT, F. C. L. G. FARIA, T. M. FARIAS, C. D. FARIAS, F. F. FARIAS, K. S. FARIAS, M. A. F. FARIAS, M. R. FARISCO, M. FATTORI, N. L. FECCHIO, D. FELIPE, C. R. FELIPPE, A. C. FERNANDES, A. B. V. FERNANDES, C. S. E.

FERNANDES, E. FERNANDES, F. M. FERNANDES, L. C. FERNANDES, L. FERNANDES, S. O. A. FERRARI, G. B. FERRARI, P. C. FERRAZ, A. P. C. R. FERRAZ, H. O. FERRAZ, L. R. M. FERREIRA, A. C. FERREIRA, A. J. FERREIRA, A. S. FERREIRA, B. L. S. M.

FERREIRA, E. P. C. B. FERREIRA, F. M. P. FERREIRA, G. S. FERREIRA, I. M. FERREIRA, J. S. FERREIRA, L. L. D. M. FERREIRA, L. S. FERREIRA, M. P. O. FERREIRA, M. R. B.

CFSP212 CFSP124 CFSP370 CFSP363 CFSP297 CFSP243, CFSP290 CFSP045 CFSP387 CFSP204 CFSP278, CFSP315 CFSP301 CFSP343, CFSP344 CFSP418 CFSP408 CFSP415 CFSP211 CFSP369 CFSP122 CFSP029, CFSP137 CFSP034, CFSP082, CFSP090, CFSP113, CFSP129, CFSP145 CFSP245 CFSP203 CFSP206 CFSP043 CFSP290 CFSP141 CFSP229 CFSP211 CFSP092 CFSP298, CFSP333 CFSP327 CFSP071, CFSP131 CFSP298, CFSP333 CFSP318, CFSP321, CFSP344, CFSP380, CFSP381 CFSP202, CFSP331 CFSP079, CFSP085, CFSP088 CFSP307 CFSP325 CFSP127 CFSP253, CFSP338, CFSP345 CFSP311 CFSP126 CFSP244

FERREIRA, P. H. FERREIRA, R. A. FERREIRA, S. B. R. B. FERREIRA, S. G. FERREIRA, T. R.

CFSP194 CFSP055 CFSP417 CFSP327 CFSP021, CFSP089, CFSP098, CFSP139, CFSP414 FERREIRA, T. CFSP419 FIDELIS, D. CFSP419 FIGUEIRA, G. W. CFSP350 FIGUEIREDO, P. M. S. CFSP311 FIGUEIREDO, P. M. CFSP265 FIGUEIREDO, T. C. S. CFSP057 FIGUEREDO JR., A. G. CFSP109, CFSP138, CFSP140, CFSP398 FILIPINI, L. A. CFSP185 FIORAMONTE, M. CFSP295 FIORAVANTE, A. CFSP375 FIRMINO, B. A. M. CFSP151 FISNACK, F. S. CFSP014, CFSP105 FONSECA, F. L. A. CFSP003, CFSP361 FONTELES, A. V. CFSP033, CFSP119, CFSP147, CFSP299, CFSP413 FONTENELE, A. E. P. CFSP147 FONTENELE, A. E. P. CFSP147, CFSP299 FONTES, P. K. CFSP335 FORGERINI, M. CFSP030 FORMAGGIO, L. C. V. CFSP271 FRAGA, G. G. A. S. CFSP380 FRANÇA, G. I. S. CFSP399 FRANCHI, F. F. CFSP335 FRANCO, Í. R. T. CFSP178 FRANCO, L. F. CFSP402 FRANCO, O. L. CFSP280 FRANCO, Y. O. CFSP366 FREITAS, K. G. CFSP101 FREITAS, M. A. S. CFSP152 FREITAS, M. S. CFSP123 FREITAS, P. D. CFSP355 FUGITA, J. S. N. CFSP132 FUJISHIMA, M. A. T. CFSP077, CFSP160 FURLANETO, A. X. CFSP096 FURTADO, D. F. CFSP019, CFSP042, CFSP044 FURTADO, R. A. CFSP262 FUSCO, A. R. J. CFSP415 G GAITANI, C. M.

CFSP224

Brazilian Journal of Pharmaceutical Sciences

AUTHOR INDEX/ÍNDICE DE AUTORES

GALANTE, M. C. GALVÃO, T. C. GALVÃO, T. F. GAMA, R. M. GARCIA, D. M. GARCIA, J. L. GARCIA, M. T. J. GARCIA, P. C. GASPAR, S. L. F. GASPARETTO, A. GAZOLA, A. C. GENVIGIR, F. D. V. GERALDES, D. C. GERALDO, L. P. GERONIMO, G. GHISLENI, D. D. M. GIGLIOLI, E. B. GIROTTO, E. GODOI, B. N. GOIS, M. I. A. GOMES, D. C. GOMES, E. C. B. S. GOMES, I. S. GOMES, R. R. A. GOMEZ, D. S. GONÇALVES, B. S. GONÇALVES, T. P. GONÇALVES, T. T. GOULART, M. O. F. GOUVEIA, T. H. N. GOZZO, F. C. GRACIANI, F. S. GRACIANO, E. GROSSO, A. F. GROTTO, D. GUAZELLI, C. F. S. GUEDES, C. E. V. GUEDES, J. P. M. GUERRA, C. C. GUERRA, L. O. GUIDONI, C. M. GUIMARÃES, L. L. GUIMARÃES, M. GUIMARÃES, R. P. GUIMARAES, S. C. N.

CFSP120, CFSP132 CFSP340 CFSP199, CFSP416 CFSP252, CFSP292, CFSP314 CFSP212 CFSP211 CFSP289 CFSP334 CFSP357 CFSP419 CFSP043, CFSP190, CFSP208, CFSP375 CFSP369 CFSP307 CFSP264 CFSP294 CFSP271 CFSP144 CFSP004, CFSP419 CFSP389 CFSP248 CFSP120 CFSP409 CFSP108 CFSP297 CFSP142, CFSP204, CFSP209 CFSP326 CFSP289 CFSP288, CFSP330 CFSP261 CFSP281 CFSP295 CFSP268, CFSP276 CFSP363 CFSP144 CFSP366 CFSP228, CFSP267 CFSP014, CFSP078, CFSP105, CFSP111 CFSP297 CFSP160 CFSP245 CFSP004 CFSP123 CFSP246 CFSP391 CFSP070, CFSP121

H HACKER, S. S. HANAI, V. M. HASSUNUMA, R. M. HATANAKA, E. HAUSCHILDT, A. T. HENRIQUE, C. Y. HERNANDEZ, C. D. HIDALGO, S. F. HILINSKI, E. G. HIRATA, M.

CFSP336, CFSP339 CFSP366 CFSP334 CFSP050 CFSP275 CFSP270, CFSP284 CFSP382 CFSP315 CFSP278, CFSP337 CFSP362, CFSP363, CFSP369 HIRATA, R. D. C. CFSP362, CFSP363, CFSP369 HIRATA, T. CFSP363 HOEPNER, L. CFSP035 HORA, B. S. CFSP404 HÚNGARO, H. M. CFSP226 I IBIAPINA, J. R. INSUE, T. N. A. N. IRICEVOLTO, P. O. ISHIKAWA, R. L.

CFSP150 CFSP372, CFSP373 CFSP101 CFSP419

J JACINTO, B. JACOB FILHO, W. JÁCOME JR., A. T. JESUS, A. C. F. JESUS, C. C. JESUS, C. P. S. JESUS, E. M. S. JESUS, M. S. JONAS, G. S. JORGE, M. P. JUNQUEIRA, B. C. M. JUSTINO, S. K. M.

CFSP129 CFSP156 CFSP317 CFSP144 CFSP341 CFSP231 CFSP404 CFSP404 CFSP117 CFSP101 CFSP200 CFSP145

K KADENÁ, T. M. C. S. KASSUYA, C. A. L. KAWAI, L. T. KERBAUY, G. KIMURA, S. S. KLABUNDE, H. KOBA, S. T. KOBAYASHI, R. K. T. KODAMA, R. KOPITTKE, L.

CFSP064 CFSP268, CFSP276 CFSP083 CFSP372, CFSP373 CFSP396 CFSP035 CFSP059 CFSP376 CFSP295 CFSP091

Brazilian Journal of Pharmaceutical Sciences

AUTHOR INDEX/ÍNDICE DE AUTORES

KOZUSNYANDREANI, D. I. KRAUSSER, M. L. F. KRIEGER, J. E. KRUPP, T. KUNIYOSHI, A. L LACERDA, A. W. L.

LACERDA, M. C. C. LADEIA, M. D. D. LAMBERTI, V. LAMEIRA, C. N.

LAMOLHA, M. A. LANAS, F. LANDIM, G. C. F. LAPCHIK, M. S. LAURENTZ, R. S. LEAL, I. C. LEANDRO, A. K. C. LEANDRO, J. C. LEÃO, A. L. LEÃO, V. F. LEDESMA, M. R. B. M. LEITE, P. F. LEITE, S. N. LEMOS, G. S.

LEMOS, L. B.

LEONARDI, G. R. LEONEL, A. H. LIMA NETO, V. B. LIMA, Á. A. N. LIMA, A. I. H. LIMA, C. G. A. LIMA, C. M. S. LIMA, E. C. LIMA, G. P. P. LIMA, J. L. LIMA, J. R. LIMA, J. S.

CFSP251 CFSP035 CFSP124, CFSP348 CFSP309 CFSP295

CFSP114, CFSP157, CFSP388, CFSP393, CFSP394, CFSP422 CFSP186 CFSP133 CFSP419 CFSP070, CFSP079, CFSP085, CFSP088, CFSP121, CFSP164, CFSP277, CFSP302 CFSP026, CFSP039 CFSP362 CFSP066, CFSP094 CFSP008 CFSP257 CFSP253, CFSP345, CFSP346 CFSP048, CFSP087 CFSP176 CFSP022, CFSP103 CFSP338 CFSP384 CFSP347 CFSP035, CFSP406, CFSP408, CFSP418 CFSP058, CFSP107, CFSP110, CFSP112, CFSP154 CFSP058, CFSP107, CFSP110, CFSP112, CFSP154 CFSP245, CFSP281 CFSP270, CFSP284 CFSP186 CFSP236, CFSP240 CFSP422 CFSP119 CFSP077, CFSP160 CFSP132 CFSP211 CFSP198 CFSP372, CFSP373 CFSP219

LIMA, J. S. LIMA, K. S. LIMA, K. S. LIMA, L. F. F. LIMA, M. F. F. LIMA, M. G. F. LIMA, M. G. R. J. LIMA, R. P.

LIMA, T. D. LIMA, T. M. LIMA, V. V. LIMA, Z. N. LIRA, A. C. LIRA, T. A. LOBATO, L. P. LOBÔ, F. M. B. S. LÔBO, M. LOPES, A. J. C. LOPES, E. M. LOPES, E. S. LOPES, F. M. LOPES, H. F. LOPES, L. C. LOPES, L. J. E. LOPES, P. S. LOPES, T. A. LOPES, T. S. P. LORANDI, P. A. LUCARINI, R. LUCAS, F. LUDWIG, I. S. LUIS, A. F. LUIZ, N. R. LUZ, T. R. S. A.

CFSP386 CFSP062, CFSP189 CFSP358 CFSP031 CFSP399 CFSP423 CFSP002 CFSP005, CFSP150, CFSP155, CFSP186, CFSP191, CFSP195, CFSP237, CFSP377 CFSP117 CFSP423 CFSP349 CFSP181, CFSP205, CFSP360 CFSP128 CFSP331 CFSP404 CFSP031 CFSP247 CFSP152 CFSP114 CFSP153 CFSP249 CFSP144 CFSP059, CFSP089, CFSP199 CFSP021, CFSP089, CFSP098, CFSP139 CFSP263, CFSP415 CFSP066 CFSP212 CFSP009 CFSP257 CFSP322 CFSP228, CFSP267 CFSP057 CFSP026 CFSP311

M MACEDO, D. H. CFSP200 MACEDO, R. S. CFSP020 MACHADO, D. CFSP233 MACHADO, F. L. S. CFSP405 MACHADO, L. P. CFSP375 MACHADO, R. R. CFSP320 MACIEL, A. P. P. CFSP150 MACIEL, L. F. CFSP215

Brazilian Journal of Pharmaceutical Sciences

AUTHOR INDEX/ÍNDICE DE AUTORES

MACIEL, S. A. S. G. MACIESKI, W. B. MADEIRA, T. B. MAGALHÃES, B. S. MAGALHÃES, G. F. MAGALHAES, S. C. G. MAGENTA, M. A. G. MANRIQUEZ, V. MANSANO, A. M. J. C. MANZINI, F. MARCATTO, L. R. MARCO, B. A. MARCUCCI, M. C. MARIN, C. C. MARINHO, I. F. S. MARINHO, M. A. D. MARINI, D. C.

MARQUES JR., J. MARQUES, A. E. F. MARQUES, A. S. MARQUES, L. C. MARQUES, L. M. MARQUES, R. A. MARQUES, S. A. MARTINEZ, C. A. R. MARTINS NETO, A. J. MARTINS, A. M. L. MARTINS, A. R. F. G.

MARTINS, É. S. F. MARTINS, J. B. MARTINS, J. S. MARTINS, L. C. MARTINS, L. P. MARTINS, N. V. N. MARTINS, P. H. MARTINS, R. A. MARTINS, R. L. MARTINS, V. A. P. MASCARENHAS, N. G. MASTROIANNI, P. C.

CFSP345, CFSP346 CFSP049 CFSP239 CFSP280 CFSP421 CFSP372, CFSP373 CFSP292 CFSP362 CFSP279 CFSP035, CFSP406 CFSP124, CFSP348 CFSP238, CFSP241 CFSP402 CFSP173 CFSP119, CFSP299 CFSP147 CFSP034, CFSP046, CFSP047, CFSP054, CFSP056, CFSP082, CFSP090, CFSP113, CFSP129, CFSP145, CFSP146 CFSP374, CFSP378 CFSP062, CFSP214 CFSP106 CFSP158, CFSP163 CFSP266 CFSP007, CFSP198 CFSP148, CFSP176, CFSP210 CFSP288 CFSP190, CFSP208 CFSP077 CFSP033, CFSP119, CFSP147, CFSP299, CFSP413 CFSP289 CFSP172 CFSP152 CFSP161 CFSP049, CFSP172, CFSP203, CFSP208 CFSP350 CFSP198 CFSP020 CFSP305, CFSP306 CFSP278, CFSP315 CFSP263 CFSP030, CFSP382, CFSP390, CFSP397

MATOS, J. C. MATOS, R. A. MAXIMINO, F. D. S. MAZA, L. MAZZOLA, P. G. MECATTI, G. C. MEDEIROS JR., J. P. MEDEIROS, M. A. S. MEDEIROS, M. MEIRA, N. A. N. MELLO, A. E. C. MELLO, A. L. M. F. MELLO, K. G. P. C. MELLO, R. J. MELO, A. F. M. MELO, C. M. MELO, C. V. MELO, E. R. MELO, I. S. F. MELO, J. L. MELO, N. S. MELO, R. S. MENARDI, M. A. P. MENDES, E. C. R. MENDES, F. R. S. MENDES, I. T. MENDES, R. C. MENDES, S. J. MENDONÇA, T. C. MENEGON, A. S. MENESES, E. C. MENESES, J. P. MENEZES FILHO, J. A. MENEZES, A. R. R. MENEZES, E. V. MENEZES, S. R. A. MENEZES, T. B. C. MESCHIATI, K. MESQUITA, A. I. M. MESSIAS, M. C. F. MESTRE, R. F. S. MIALHE, F. L. MICHELIN, D. L. M. MINARINI, P. R. R. MINATEL, I. O. MINTO, B. A.

CFSP161 CFSP063 CFSP032, CFSP118 CFSP086 CFSP416 CFSP288 CFSP033, CFSP413 CFSP005, CFSP186, CFSP237 CFSP293 CFSP215, CFSP247 CFSP026 CFSP057 CFSP244, CFSP332, CFSP384 CFSP341 CFSP230, CFSP344, CFSP354, CFSP381 CFSP253, CFSP338 CFSP219, CFSP399 CFSP010, CFSP015 CFSP320 CFSP076, CFSP084 CFSP026 CFSP090 CFSP125 CFSP376 CFSP331 CFSP312 CFSP300 CFSP408, CFSP423 CFSP233, CFSP283 CFSP120, CFSP132 CFSP078 CFSP029, CFSP137, CFSP201 CFSP215 CFSP309 CFSP340 CFSP310, CFSP329 CFSP011 CFSP111 CFSP388 CFSP288, CFSP330 CFSP248 CFSP407 CFSP175 CFSP415 CFSP211 CFSP395

Brazilian Journal of Pharmaceutical Sciences

AUTHOR INDEX/ÍNDICE DE AUTORES

MIRANDA, A. S. CFSP088 MIRANDA, I. R. CFSP376 MIRANDA, K. H. O. CFSP404 MIRANDA, L. S. CFSP133 MIRANDA, M. C. CFSP160 MIRANDA, P. S. CFSP113 MIRANDA, R. S. CFSP365 MONTANHA, M. M. C. CFSP203 MONTE, B. F. CFSP051 MONTEIRO JR., E. B. CFSP325 MONTEIRO, A. L. C. CFSP389 MONTEIRO, G. M. C. CFSP415 MONTEIRO, K. A. CFSP389 MONTEIRO, O. S. CFSP311 MORA, A. G. CFSP288, CFSP330 MORAES, A. C. L. N. CFSP150, CFSP155, CFSP191, CFSP195, CFSP377 MORAES, A. C. V. CFSP116, CFSP192 MORAES, A. G. CFSP145 MORAES, A. L. G. CFSP367 MORAES, D. N. CFSP251, CFSP322 MORAES, E. V. CFSP389 MORAES, J. C. CFSP245 MORAES, J. D. D. CFSP245 MORAES, M. A. CFSP258 MORAIS, M. Í. CFSP320 MORANDI, A. C. CFSP109, CFSP138, CFSP140, CFSP398 MOREIRA, H. G. CFSP407 MOREIRA, V. C. S. CFSP029, CFSP201 MORENO, A. H. CFSP220, CFSP308 MORENO, G. G. B. CFSP412 MORETTI, A. B. F. CFSP251 MORGUETTE, A. E. B. CFSP217 MORI, A. L. P. M. CFSP423 MORIEL, P. CFSP028, CFSP141 MOTA K. L. CFSP052 MOTA, I. V. R. CFSP058, CFSP107, CFSP110, CFSP112, CFSP154 MOTTA, A. G. C. CFSP216 MOTTA, J. M. CFSP144 MOURA JR., M. A. CFSP234, CFSP269 MOURA, M. R. C. G. CFSP248 MOURA, R. O. CFSP181, CFSP205, CFSP360 MOURA, W. C. S. CFSP181, CFSP205, CFSP360 MÜLLER, D. E. CFSP216

MUNIZ, D. H. C. MUNIZ, I. N. S. MURAKAMI, H. N. F. MUZITANO, M. F.

N NABARRETTI, B. H. NADER, T. T. NAGANO, M. S. NAKADA, K. K. NAKAWAGA, C. M. NASCIMENTO, A. A. NASCIMENTO, A. C. NASCIMENTO, C. S. NASCIMENTO, F. I. S. NASCIMENTO, I. R. C. NASCIMENTO, J. C. NASCIMENTO, L. O. NASCIMENTO, L. O.

NASCIMENTO, M. V. S. Q. NASCIMENTO, W. M. C. NASSAR, E. J. NAVA, R. G. NAVARRO, L. C. NAVARRO, R. S. NEPOMUCENO, C. M. NÉRI, E. D. R. NERY, A. A. NERY, M. M. F. NEUBAUER, I. W. NEVES, J. K. O. NEVES, S. J. F.

NICÁCIO, R. A. F. NICHIATA, L. Y. I. NICOLETTI, M. A. NIXDORF, S. L. NOBRE, T. M. M. NOGUEIRA, G. P. NOGUEIRA, I. A. L. NOGUEIRA, R. B. NOGUEIRA, S. B.

CFSP109, CFSP138, CFSP140, CFSP398 CFSP230, CFSP381, CFSP344 CFSP083 CFSP253, CFSP341, CFSP345, CFSP346

CFSP245 CFSP270, CFSP284 CFSP232 CFSP083 CFSP373 CFSP299 CFSP073 CFSP144 CFSP198 CFSP410 CFSP069 CFSP168 CFSP221, CFSP293, CFSP307, CFSP403, CFSP416 CFSP143 CFSP051, CFSP421 CFSP257 CFSP376 CFSP290, CFSP243 CFSP251 CFSP084 CFSP114, CFSP393, CFSP394 CFSP110, CFSP154 CFSP239, CFSP272 CFSP008 CFSP010, CFSP015 CFSP018, CFSP022, CFSP023, CFSP093, CFSP099, CFSP103 CFSP048, CFSP087 CFSP350 CFSP387, CFSP423 CFSP239 CFSP191, CFSP195 CFSP039 CFSP019, CFSP042, CFSP044 CFSP053, CFSP166 CFSP417

Brazilian Journal of Pharmaceutical Sciences

AUTHOR INDEX/ÍNDICE DE AUTORES

NONATO, Í. N. F. NOVAIS, G. P. NUCCI, L. B. NUNES, C. R. M. NUNES, J. P. NUTELS, T. O OHASHI, L. H. OKAMOTO, R. T. OLEGARIO, R. S. B. OLIVEIRA FILHO, A. D.

OLIVEIRA, A. A. S. OLIVEIRA, A. L. Q. OLIVEIRA, A. M. R. R. OLIVEIRA, A. M. OLIVEIRA, A. P. S. OLIVEIRA, B. C. OLIVEIRA, B. E. OLIVEIRA, C. A. OLIVEIRA, C. H. B. OLIVEIRA, C. R. OLIVEIRA, C. S. OLIVEIRA, F. M. OLIVEIRA, G. I. OLIVEIRA, H. C. OLIVEIRA, I. S. OLIVEIRA, J. C. OLIVEIRA, J. M. S. OLIVEIRA, J. S. S. OLIVEIRA, J. OLIVEIRA, L. C. F. OLIVEIRA, L. S. OLIVEIRA, M. M. OLIVEIRA, M. R. OLIVEIRA, P. G. OLIVEIRA, R. B. OLIVEIRA, R. M. OLIVEIRA, R. P. OLIVEIRA, R. OLIVEIRA, S. F. M. OLIVEIRA, S. S. P.

CFSP415 CFSP415 CFSP411 CFSP368 CFSP096 CFSP018, CFSP093

CFSP061, CFSP364 CFSP271 CFSP079 CFSP018, CFSP022, CFSP023, CFSP048, CFSP093, CFSP099, CFSP103 CFSP230, CFSP344, CFSP381 CFSP052 CFSP204 CFSP059, CFSP199, CFSP395 CFSP054, CFSP056 CFSP212 CFSP219 CFSP184, CFSP167 CFSP375 CFSP097, CFSP212, CFSP402 CFSP092 CFSP030 CFSP171 CFSP163 CFSP121, CFSP164, CFSP277, CFSP302 CFSP032 CFSP404 CFSP350 CFSP065, CFSP125, CFSP185 CFSP125 CFSP011 CFSP009 CFSP026 CFSP020 CFSP320 CFSP170 CFSP132 CFSP363 CFSP344 CFSP215

OLIVEIRA, T. F. R. OLIVEIRA, V. C. B. OTAGUIRI, E. S. OYA, C. T. P PADUA, C. A. M. PAGANINI, A. PAGANINI, W. S. PAGANOTTE, D. M. PAIM, R. S. P. PAIVA, J. S. PAIXÃO, B. B. PAIXÃO, Y. D. L. PARDI, P. C. PARMA, M. L. G. PASOTTI, D. V. PASSETI, T. A. PASSONI, M. H. PAULA, B. A. P. PAULA, D. PAULA, E.

PAULA, F. M. S. F. PAULA, J. F. P. PAULA, M. C. PAULA, M. PAULA, V. C. PAULI, M. C. PAULO, L. A. PAVANELLI, W. R. PECCIN, M. S. PEDROSO, F. M. PEDROSO, J. V. M. PENTEADO, É. PEREIRA NETTO, E. S. PEREIRA, A. C. PEREIRA, A. G. S. PEREIRA, A. M. N. PEREIRA, A. M. S. PEREIRA, A. S. PEREIRA, B. S. PEREIRA, D. N. PEREIRA, F. G. F. PEREIRA, G. R.

CFSP038 CFSP318, CFSP321, CFSP351 CFSP217 CFSP326

CFSP112 CFSP296 C FSP385, CFSP401 CFSP197 CFSP168 CFSP143 CFSP082 CFSP230, CFSP354, CFSP381 CFSP074 CFSP226, CFSP282 CFSP420 CFSP003, CFSP250, CFSP361 CFSP281 CFSP417 CFSP160 CFSP221, CFSP233, CFSP283, CFSP287, CFSP294 CFSP296 CFSP286 CFSP182 CFSP098 CFSP057 CFSP281 CFSP258 CFSP376 CFSP020 CFSP163 CFSP059 CFSP347 CFSP386 CFSP124, CFSP348 CFSP108 CFSP350 CFSP270, CFSP284 CFSP055 CFSP211 CFSP360 CFSP114 CFSP312

Brazilian Journal of Pharmaceutical Sciences

AUTHOR INDEX/ÍNDICE DE AUTORES

PEREIRA, J. P. A. PEREIRA, M. A. PEREIRA, M. D. PEREIRA, M. K. PEREIRA, M. PEREIRA, N. P. PEREIRA, P. S. PEREIRA, R. M. S. PEREIRA, R. M. PEREIRA, T. C. PEREIRA, T. P. PEREIRA, U. R. S. PERINI, E. PERRELLA, I. M. PERUGINI, L. F. PERUGINI, M. R. E. PESSOA, E. T. F. P. PESTANA, J. O. M. PETRI, G. PETRIS, A. J. PETRONI, D. S. PIANETTI, G. A. PICANÇO A. L. R. PICCININI, A. PICOLO, G. PIEDADE, N. S. PIERDONÁ, T. M. PIERI, E. PIMENTA, A. C. PINHEIRO, A. A. PINHEIRO, N. N. O.

PINHEIRO, R. M. PINHEIRO, T. F. PINHO, M. V. T. PINHO, R. M. L. PINTO, F. F. PINTO, G. R. PINTO, M. A. O. PINTO, N. B. PINTO, S. C. PINTO, T. J. A. PONDÉ, P. D. S. PONTES, K. S. PONTES, R. G. M. S. POPOV, C. S. F. C.

CFSP188 CFSP180 CFSP386 CFSP208 CFSP370 CFSP215, CFSP247 CFSP090 CFSP158 CFSP096 CFSP325 CFSP076, CFSP084, CFSP128, CFSP392 CFSP076 CFSP112 CFSP225 CFSP376 CFSP372, CFSP373 CFSP052 CFSP369 CFSP252, CFSP361 CFSP419 CFSP200 CFSP242 CFSP377 CFSP043, CFSP208 CFSP295 CFSP313 CFSP002, CFSP152, CFSP266 CFSP370 CFSP071 CFSP305, CFSP306 CFSP114, CFSP157, CFSP388, CFSP393, CFSP394, CFSP422 CFSP133 CFSP032 CFSP083 CFSP225 CFSP054, CFSP056 CFSP014, CFSP078 CFSP226 CFSP062, CFSP214 CFSP304 CFSP271, CFSP337 CFSP024 CFSP414 CFSP341, CFSP345, CFSP346 CFSP280

PORTARO, F. PORTO, N. K. A. PRADO, A. A. R. PRADO, G. M. PRATES, S. S. PRESTES, E. PRUDENTE, L. R.

CFSP295 CFSP301 CFSP355 CFSP421 CFSP326 CFSP229 CFSP068

Q QUEIROZ, M. J. S. QUEIROZ, T. A. QUEIROZ, V. C. QUINTANS JR., L. J. QUIRINO, T. P. M. B.

CFSP070, CFSP164 CFSP009 CFSP003 CFSP236, CFSP240 CFSP214

R RABELO, É. M. RAFAEL, A. L. B. G. RAIMUNDO, J. M.

RAMALHO, Í. M. M. RAMOS, E. A. RAMOS, L. E. O. RAMOS, S. P. RANGEL, R. L. P. RAZZA, E. M. REBECH, I. RECH, N. REIS, E. P. REIS, T. B. P. REMONDI, F. A. RENNÓ, A. L. RESENDE, E. M. REZENDE, L. H. O. REZENDE, R. A. E. REZENDE, T. M. B. REZENDE, T. S. RIBAS, A. E. B. RIBEIRO NETO, L. M. RIBEIRO, B. S. RIBEIRO, K. A. RIBEIRO, K. O. RIBEIRO, L. N. M.

RIBEIRO, M. A. L. RIBEIRO, N. C.

CFSP305, CFSP306 CFSP356 CFSP253, CFSP304, CFSP338, CFSP341, CFSP345, CFSP346 CFSP236, CFSP240 CFSP039 CFSP079 CFSP340, CFSP358 CFSP413 CFSP335 CFSP228, CFSP267 CFSP406 CFSP144 CFSP219 CFSP004, CFSP419 CFSP096 CFSP158 CFSP182 CFSP026 CFSP280 CFSP248 CFSP148, CFSP176, CFSP210 CFSP016, CFSP036, CFSP069 CFSP134 CFSP111 CFSP212 CFSP221, CFSP233, CFSP283, CFSP287, CFSP294 CFSP404 CFSP051

Brazilian Journal of Pharmaceutical Sciences

AUTHOR INDEX/ÍNDICE DE AUTORES

RIBEIRO, N. F. RIBEIRO, R. A. RIBEIRO, S. C. C. RICCIO, B. V. F. RIGON, D. A. RIOLI, V. RIVELLI, G. G. RIVERA, J. G. B. ROCHA JR., S. N. ROCHA, A. L. O. ROCHA, C. Q. ROCHA, C. S. ROCHA, E. D. ROCHA, E. M. ROCHA, E. S. ROCHA, M. C. ROCHA, P. S. S. ROCHA, S. M. RODARTE, M. P. RODRIGUES, A. B. L. RODRIGUES, A. C. J. RODRIGUES, F. F. RODRIGUES, L. N. C. RODRIGUES, M. V. RODRIGUES, R. B. RODRIGUES, R. L.

RODRIGUES, V. A. V. ROLIM NETO, P. J. ROLIM, L. A. ROMAN, C. ROMERA J. F. ROMEU, G. A. RONDINELLI, C. ROSA, B. C. D. ROSA, B. P. P. ROSA, M. B. ROSA, P. C. P. ROSSATTO, A. ROSSI, E. M. ROSSI, M. E. ROSSI, M. S. P. N. RUELA, A. L. M. RUFINO, J. V. RUGGIERO, A. A.

CFSP331 CFSP338 CFSP100 CFSP229, CFSP286 CFSP040 CFSP295 CFSP242 CFSP357 CFSP371 CFSP232 CFSP311 CFSP206 CFSP326 CFSP053, CFSP162, CFSP166, CFSP174 CFSP311 CFSP293 CFSP027, CFSP081 CFSP377 CFSP226, CFSP282 CFSP305, CFSP306 CFSP017 CFSP320 CFSP235 CFSP365 CFSP419 CFSP034, CFSP082, CFSP090, CFSP113, CFSP129, CFSP145 CFSP395 CFSP298, CFSP333 CFSP298, CFSP333 CFSP091 CFSP029 CFSP155 CFSP396 CFSP216 CFSP375 CFSP112 CFSP416 CFSP342 CFSP108 CFSP250 CFSP423 CFSP312 CFSP239, CFSP272 CFSP116, CFSP173, CFSP192, CFSP250, CFSP252

S SAAVEDRA, N. SABINO, W. SACILOTTO, L. SAI, L. C. SAKAI, M. C. SALAZAR, A. B. C. SALAZAR, L. SALES, C. A. SALES, C. C. SALGADO, H. R. N. SALGUEIRO, F. R. SALLES, L. SALLES, M. J. S. SALVI JR., A. SALVIANO, G. SAMPAIO, R. I. A. F. SANT ANNA, T. F. P. SANTANA, A. R. G. SANTANA, C. A. SANTANA, F. J. M. SANTANA, L. C. SANTELLO, F. H. SANTOS, A. C. N. SANTOS, A. L. G. O. SANTOS, B. C. S. SANTOS, B. D. SANTOS, B. S. SANTOS, C. F. SANTOS, D. N. C. SANTOS, D. S. SANTOS, G. A. A. SANTOS, G. F. SANTOS, I. C. R. SANTOS, I. S. SANTOS, J. A. SANTOS, J. A. SANTOS, J. D. L. SANTOS, J. F. R. SANTOS, J. M. SANTOS, J. O. SANTOS, K. C. SANTOS, L. A. SANTOS, L. M. R. SANTOS, L. SANTOS, M. E. R. SANTOS, M. H. C.

CFSP362 CFSP108 CFSP124 CFSP264 CFSP396 CFSP369 CFSP362 CFSP357 CFSP263 CFSP224, CFSP238, CFSP241, CFSP274 CFSP105 CFSP086 CFSP340, CFSP358 CFSP197, CFSP279, CFSP296, CFSP420 CFSP132 CFSP052 CFSP200 CFSP317 CFSP074, CFSP122 CFSP057 CFSP013, CFSP115 CFSP102 CFSP248 CFSP121 CFSP282 CFSP309 CFSP297 CFSP020 CFSP210 CFSP218 CFSP074 CFSP054 CFSP327 CFSP359 CFSP109, CFSP138, CFSP140, CFSP398 CFSP268, CFSP276 CFSP261 CFSP361 CFSP378, CFSP379 CFSP292 CFSP211 CFSP034 CFSP130 CFSP063, CFSP180 CFSP055 CFSP206

Brazilian Journal of Pharmaceutical Sciences

AUTHOR INDEX/ÍNDICE DE AUTORES

SANTOS, M. L. L. SANTOS, N. J. S. SANTOS, N. R. SANTOS, P. C. J. L. SANTOS, R. A. SANTOS, R. B. SANTOS, S. C. S. SANTOS, S. L. F.

SANTOS, S. R. C. J. SANTOS, V. L. SANTOS, W. B. SARAIVA, E. M. S. SARAIVA, R. L. V. SATO, C. M. A. SATO, S. A. SCANAVACCA, M. I. SCANAVACHI, C. M. B. SCHEPER, R. M. SCHERF, J. R. SCUSSEL, R. SEGHETO, L. SEGUNDO, M. A. S. P. SERAPHIM, A. C. V. SERON, P. SESTARIO, C. S. SHIL, D. P. SHIO, M. T. SIBIONI, D. SIEBRA, P. S. C. SILVA FILHO, A. A. SILVA FILHO, C. R. SILVA FILHO, J. B. SILVA FILHO, J. C. B. SILVA JR., C. V. SILVA JR., H. T. SILVA JR., J. M. SILVA NETO, G. J. SILVA, A. A. SILVA, A. B. G. SILVA, A. C. Z. SILVA, A. C. SILVA, A. E. S. SILVA, A. F. M. SILVA, A. F.

CFSP285 CFSP058, CFSP107, CFSP110, CFSP154 CFSP206 CFSP124, CFSP348 CFSP025 CFSP137 CFSP164, CFSP277 CFSP114, CFSP157, CFSP388, CFSP393, CFSP394, CFSP422 CFSP142, CFSP204, CFSP209 CFSP220, CFSP308 CFSP165 CFSP368 CFSP051 CFSP111 CFSP083 CFSP124, CFSP348 CFSP046 CFSP049 CFSP368 CFSP370 CFSP282 CFSP205, CFSP360 CFSP187 CFSP362 CFSP358 CFSP352 CFSP095 CFSP419 CFSP147 CFSP257 CFSP142 CFSP249 CFSP317, CFSP380 CFSP142, CFSP204, CFSP209 CFSP369 CFSP204, CFSP209 CFSP410 CFSP311, CFSP256 CFSP316, CFSP259 CFSP014, CFSP105, CFSP111 CFSP298, CFSP333 CFSP077 CFSP402 CFSP080

SILVA, A. L. SILVA, A. M. R. SILVA, A. R. A. SILVA, A. R. A. SILVA, A. R. N. SILVA, A. R. P. SILVA, A. R. SILVA, A. S. U. SILVA, B. B. SILVA, B. C. SILVA, C. C. SILVA, C. H. B. SILVA, C. I. F. SILVA, C. I. S. M. SILVA, C. L. SILVA, D. D. S. SILVA, D. D. S. SILVA, D. M. SILVA, E. J. P. SILVA, E. M. SILVA, E. R. SILVA, E. S. SILVA, F. B. SILVA, F. C. Q. SILVA, F. M. SILVA, F. P. L. SILVA, F. R. SILVA, F. T. SILVA, F. V. SILVA, G. H. R. SILVA, G. K. A. SILVA, H. M. SILVA, I. C. V. SILVA, J. A. S. SILVA, J. C. R. A. SILVA, J. D. N. SILVA, J. F. SILVA, J. F. SILVA, J. G. G. SILVA, J. L. P.

SILVA, J. M. F. SILVA, J. M. SILVA, J. R. G.

CFSP219 CFSP004 CFSP237 CFSP331 CFSP164, CFSP277, CFSP302, CFSP070 CFSP061 CFSP005, CFSP195 CFSP027, CFSP081 CFSP246 CFSP056 CFSP029 CFSP043, CFSP190 CFSP351 CFSP007 CFSP237, CFSP377 CFSP120 CFSP298, CFSP333 CFSP165 CFSP121 CFSP033 CFSP266 CFSP379, CFSP374, CFSP378 CFSP019, CFSP044, CFSP042, CFSP265 CFSP239, CFSP272 CFSP144 CFSP337 CFSP328 CFSP256 CFSP254 CFSP233, CFSP283, CFSP294 CFSP329, CFSP310 CFSP266 CFSP346 CFSP218, CFSP219 CFSP400 CFSP053 CFSP137 CFSP300, CFSP353 CFSP323 CFSP318, CFSP343, CFSP351, CFSP354, CFSP380 CFSP259, CFSP316 CFSP360 CFSP230, CFSP351, CFSP354, CFSP356

Brazilian Journal of Pharmaceutical Sciences

AUTHOR INDEX/ÍNDICE DE AUTORES

SILVA, J. R. SILVA, J. S. F. SILVA, K. B. SILVA, L. A. SILVA, L. A. SILVA, L. C. C. SILVA, L. C. SILVA, L. L. F. SILVA, L. L. SILVA, L. M. S. SILVA, L. M. SILVA, M. A. B. SILVA, M. A. S. SILVA, M. A. V. SILVA, M. C. SILVA, M. F. B. SILVA, M. J. D. SILVA, M. L. A. SILVA, M. N. SILVA, M. T. SILVA, M. V. SILVA, M. V. SILVA, M. Z. SILVA, N. C. SILVA, N. L. L. SILVA, N. L. SILVA, N. M. SILVA, N. R. S. SILVA, N. S. R. SILVA, N. S. SILVA, P. M. SILVA, R. A. S. A. SILVA, R. B. SILVA, R. C. B. SILVA, R. M. F. SILVA, R. N. C. SILVA, R. S. SILVA, R. Z. SILVA, S. A. SILVA, S. G. G. SILVA, S. M. A. SILVA, S. M. SILVA, S. N.

CFSP309 CFSP321, CFSP356 CFSP174 CFSP012, CFSP273 CFSP317 CFSP226 CFSP143 CFSP231 CFSP253 CFSP019, CFSP042, CFSP044, CFSP265 CFSP402 CFSP043 CFSP109, CFSP138, CFSP140, CFSP398 CFSP367, CFSP407 CFSP079, CFSP085, CFSP088 CFSP216 CFSP234, CFSP269 CFSP262, CFSP257 CFSP277 CFSP021, CFSP059, CFSP089, CFSP199 CFSP027, CFSP081 CFSP259, CFSP316, CFSP391 CFSP038, CFSP060 CFSP148, CFSP176, CFSP210 CFSP357 CFSP076, CFSP084, CFSP260 CFSP057, CFSP222 CFSP254 CFSP077 CFSP144 CFSP334 CFSP189 CFSP180 CFSP350 CFSP298, CFSP333 CFSP003 CFSP075 CFSP023, CFSP099 CFSP174 CFSP077 CFSP410 CFSP075 CFSP256

SILVA, S. O. SILVA, T. B. C. SILVA, T. F. B. SILVA, T. M. SILVA, T. R. SILVA, U. D. A. SILVA, V. A. B. SILVA, V. A. F. SILVA, V. B. SILVA, V. P. B. SILVA, V. R. L. SILVA, W. P. C. SILVEIRA, A. C. F. SILVEIRA, P. F. SILVERIO, R. M. SINGH, A. K. SIQUEIRA JR., O. Q. SIQUEIRA, R. M. P. SIQUETO, F. R. SIVIERO, B. R. SOARES, B. G. M. R.

SOARES, P. SOARES, R. A. G. SOARES, R. F. SOARES, V. C. G.

SOLON, L. G. S. SONCINI, J. G. M. SOUSA, F. A. SOUSA, F. F. O. SOUSA, J. S. SOUSA, O. V. SOUSA, R. A. SOUSA, S. M. O. SOUTO, P. C. S. SOUZA NETO, R. C. SOUZA, A. G. SOUZA, A. J. C. SOUZA, A. P. C. SOUZA, A. S. SOUZA, C. E. M. SOUZA, G. H. J. SOUZA, J. E. P. SOUZA, J. R. SOUZA, L. A.

CFSP402 CFSP167, CFSP184 CFSP060 CFSP001, CFSP037 CFSP036 CFSP077 CFSP209 CFSP212 CFSP216 CFSP310, CFSP329 CFSP263 CFSP085, CFSP088, CFSP302 CFSP339 CFSP388 CFSP068 CFSP255 CFSP262 CFSP421 CFSP402 CFSP246 CFSP033, CFSP119, CFSP147, CFSP299, CFSP413 CFSP118 CFSP348 CFSP321, CFSP356 CFSP063, CFSP148, CFSP159, CFSP176, CFSP180, CFSP210 CFSP313 CFSP376 CFSP152 CFSP227, CFSP313 CFSP070 CFSP282 CFSP396 CFSP392 CFSP309 CFSP386 CFSP405 CFSP323, CFSP324 CFSP396 CFSP039 CFSP310, CFSP329, CFSP399 CFSP264 CFSP095 CFSP039 CFSP386

Brazilian Journal of Pharmaceutical Sciences

AUTHOR INDEX/ÍNDICE DE AUTORES

SOUZA, M. C. SOUZA, M. L. SOUZA, M. S. A. SOUZA, N. C. SOUZA, N. P. S. SOUZA, R. N. SOUZA, V. K. SPINDOLA, D. G. SPÓSITO, M. A. L. STEINER, B. STEPHANO, M. A. STORPIRTIS, S. STRECK, E. S. STRUNZ, C. M. C. SUCHARA, E. A. SUGAWARA, A. M. SUTTO, A. C. P. SVICERO, B. S. SZYMKO, T. B. S. T TABOSA, M. G. O. TAKAHASHI, E. A. S. TAKAHASHI, M. M. TAKARA, R. M. TAMASHIRO, L. Y. TAMOYESE, V. M. TANIDA, L. TANO, Z. N. TAVARES JR., A. G. TAVARES, A. C. M. TAVARES, D. C. TAVARES, G. D. TAVARES, L. C. TAVARES, M. G. R. TEIXEIRA, A. B.

TEIXEIRA, H. C. A. TEIXEIRA, J. P. TEIXEIRA, L. B. TEIXEIRA, T. C. TEIXEIRA, T. W. TENIS, M. A. K. S. G. TIROLLA, R. M. TODESCHINI, D. TOLEDO, J. H.

CFSP008 CFSP218, CFSP219 CFSP402 CFSP309 CFSP156 CFSP141 CFSP204 CFSP097 CFSP403 CFSP370 CFSP336, CFSP339 CFSP041, CFSP179, CFSP408, CFSP423 CFSP091 CFSP124, CFSP348 CFSP374, CFSP378, CFSP379 CFSP106, CFSP212 CFSP111 CFSP109, CFSP138, CFSP140, CFSP398 CFSP229

CFSP343, CFSP356 CFSP075 CFSP069 CFSP326 CFSP402 CFSP340 CFSP419 CFSP376 CFSP313 CFSP235 CFSP262 CFSP293 CFSP348 CFSP028 CFSP076, CFSP084, CFSP128, CFSP260, CFSP266, CFSP392 CFSP349 CFSP302 CFSP152 CFSP086 CFSP389 CFSP008 CFSP373, CFSP372 CFSP415 CFSP403

TOLEDO, M. I. TOLEDO, O. R. TOMA, W. TORRAGA, L. F. TORRES, F. TORRES, L. C. S. TORTELOTE, T. T. TÓTOLI, E. G. TREZZA NETTO, J. TRIBOLI, E. P. R. TRICHES, D. L. G. F. TRINDADE, C. B. TRINDADE, V. Z. TROMBETA, F. B. TRUJILLO, L. M. TURATI, J. M. M. TURKÓCIO, R. I.

CFSP059 CFSP389 CFSP123, CFSP303 CFSP143 CFSP162 CFSP026 CFSP127 CFSP224, CFSP274 CFSP050 CFSP244 CFSP355 CFSP097 CFSP106 CFSP239 CFSP278, CFSP315 CFSP252 CFSP335

U UCHÔA, L. S. UNFER, T. C. URATA, M. M. F.

CFSP052, CFSP331 CFSP404 CFSP334

V VALE, V. V. VALENTE, J. G. VALENTE, M. A. VALENTE, M. G. VARALLO, F. R. VARELLA, R. L. VASCONCELOS, C. C. VASCONCELOS, I. C. T.

VASCONCELOS, S. B. VAZ, J. A. VAZ, M. M. VAZQUEZ, V. L. VENTURA, D. M. VERIDIANO, J. M. VERRI JR., W. A. VESPERO, E. C. VIANA, L. M. A. T. VIANA, M. D. M. VIANA, S. S. C. VIANNA, M. M. VICENTE, L. G. VIEIRA JR., W. F.

CFSP364 CFSP405 CFSP172, CFSP203 CFSP008 CFSP382 CFSP217 CFSP261 CFSP318, CFSP343, CFSP351, CFSP354, CFSP380 CFSP230, CFSP321, CFSP354 CFSP320 CFSP108 CFSP001, CFSP037 CFSP405 CFSP252, CFSP361 CFSP228, CFSP267 CFSP376 CFSP019, CFSP042, CFSP044 CFSP410 CFSP100, CFSP156 CFSP327 CFSP228, CFSP267 CFSP281

Brazilian Journal of Pharmaceutical Sciences

AUTHOR INDEX/ÍNDICE DE AUTORES

VIEIRA, C. L. C. VIEIRA, J. L. F. VIEIRA, J. S. B. C. VIEIRA, M. D. VILEGAS, W. VILELA, F. M. P. VILELA, W. R. VILLIS, P. C. M. VISMARI, L. VISSER, P. M. VITORINO, F. R. C. G. VRANCEANU, C. B.

CFSP052, CFSP202, CFSP331, CFSP031 CFSP357 CFSP409 CFSP038, CFSP060 CFSP234, CFSP269 CFSP282 CFSP169 CFSP265 CFSP144 CFSP411 CFSP349 CFSP312

W WADT, M. WADT, N. S. Y. WEIMANN, E. WERNECK, A. F. L. WESSLING, M. I.

CFSP200 CFSP200, CFSP254 CFSP050 CFSP226, CFSP282 CFSP231

X XAVIER, J. A. XAVIER, R. M. F.

CFSP261 CFSP006

Y YAMADA-OGATTA, S. F. CFSP217 YAMASHIRO-KANASHIRO, E. H. CFSP293 YAMAUCHI, L. M. CFSP217 YOSHIDA, E. H. CFSP366 YOSHIOKA, S. A. CFSP325 YUDICE, E. D. C. CFSP315 YUHARA, T. T. CFSP006, CFSP383 YUN, A. E. H. K. CFSP317 Z ZEFERINO, F. M. P. S. ZENAIDE, F. S. ZERBINATTI, V. R. ZUIM, N. R. B.

CFSP053, CFSP162, CFSP166, CFSP174 CFSP008 CFSP130 CFSP034, CFSP082, CFSP090, CFSP113, CFSP129, CFSP145

Revista Brasileira de Ciências Farmacêuticas Brazilian Journal of Pharmaceutical Sciences

Scientific Editor Elizabeth Igne Ferreira Editorial Board Profa. Carlota de Oliveira Rangel Yagui Prof. Jorge Mancini Filho - Supl. Prof. Joilson O. Martins Profa. Silvya Stucchi M. Engler - Supl. Prof. Michele Vitolo Associated Editors Profa. Carlota de Oliveira Rangel Yagui Profa. Cristina Helena dos Reis Serra Prof. Eliezer Jesus de Lacerda Barreiro Prof. Félix Carvalho Prof. Flávio Finardi

Editorial Advisory Board

Adriano Delfini Andricopulo Alan Douglas Kinghorn Anton Joseph Hopfinger Antonio Flávio Mídio Antonio Monge Vega Antônio Salatino Attilio Converti Camille-Georges Wermuth Carol Hollingworth Collins Dulcinéia Saes Parra Abdalla Elfrides Eva Scherman Schapoval Eliezer de Jesus Lacerda Barreiro Eloir Paulo Schenkel Francisco José Baptista Veiga Franco Maria Lajolo Isac de Almeida Medeiros Isarita Martins Ivan da Rocha Pitta João Luis Callegari Lopes Jorge Mancini Filho Lolita Margareta Tsanaclis Marcos Alcocer Maria Helena Gil Maria Inês Rocha Miritello Santoro Maria José Soares Mendes Gianini Maria Nella Gai Marilene De Vuono Camargo Penteado Massayoshi Yoshida Michele Vitolo Mumtaz Iscan Paulo César Stringheta Richard A. Glennon Robert Dennis Tanner Roy Edward Bruns Rui Curi Seizi Oga Sílvia Berlanga de Moraes Barros Silvia Storpirtis Suely Vilela Tasso Moraes e Santos Tudor Oprea Victor Manuel Cardoso Figueiredo Balcão Yves Le Loir

Prof. Joilson O. Martins Prof. Jorge Mancini Filho Prof. Luis Antonio Salazar Navarrete Prof. Luiz Antonio Gioielli Prof. Michele Vitolo Profa. Priscila Gava Mazzola Profa. Sílvia Berlanga de Moraes Barros Profa. Sílvia Storpirtis Profa. Silvya Stucchi M. Engler Profa. Vladi Olga Consiglieri Technical and Administrative Support Divisão de Biblioteca e Documentação do Conjunto das Químicas da USP

Instituto de Física de São Carlos/Universidade de São Paulo College of Pharmacy/The Ohio State University, USA College of Pharmacy/University of Illinois at Chicago, USA Faculdade de Ciências Farmacêuticas/Universidade de São Paulo (aposentado) Facultad de Farmacia/Universidad de Navarra, España Instituto de Biociências/Universidade de São Paulo Universitá Degli Studi di Genova, Italy Prestwick Chemical, France Instituto de Química/Universidade Estadual de Campinas Faculdade de Ciências Farmacêuticas/Universidade de São Paulo Faculdade de Farmácia/Universidde Federal do Rio Grande do Sul Faculdade de Farmácia/Universidade Federal do Rio de Janeiro Centro de Ciências da Saúde/Universidade Federal de Santa Catarina Faculdade de Farmácia/Universidade de Coimbra, Portugal Faculdade de Ciências Farmacêuticas/Universidade de São Paulo Centro de Ciências da Saúde/Universidade Federal da Paraíba Faculdade de Farmácia/Universidade Federal de Alfenas Centro de Ciências Biológicas/Universidade Federal de Pernambuco Faculdade de Ciências Farmacêuticas/Universidade de São Paulo, Ribeirão Preto Faculdade de Ciências Farmacêuticas/Universidade de São Paulo Cansford Lab. Limited (UK) e Laboratórios Chromatox Ltda. (São Paulo) School of Biosciences/University of Nottingham, United Kingdom Faculdade de Ciências e Tecnologia/Universidade de Coimbra,Portugal Faculdade de Ciências Farmacêuticas/Universidade de São Paulo (aposentada) Faculdade de Ciências Farmacêuticas/UNESP“Julio de Mesquita Filho”, Araraquara Facultad de Ciencias Quimicas y Farmacéuticas/Universidad de Chile, Chile Faculdade de Ciências Farmacêuticas/Universidade de São Paulo Instituto de Química/Universidade de São Paulo (aposentado) Faculdade de Ciências Farmacêuticas/Universidade de São Paulo Faculty of Pharmacy/Ankara University, Turkey Centro de Ciências Biológicas e da Saúde/Universidade Federal de Viçosa School of Pharmacy/Virginia Commonwealth University, USA Vanderbilt University, USA (Emeritus professor) Instituto de Química/Universidade Estadual de Campinas Instituto de Ciências Biomédicas/Universidade de São Paulo Faculdade de Ciências Farmacêuticas/Universidade de São Paulo (aposentado) Faculdade de Ciências Farmacêuticas/Universidade de São Paulo (aposentado) Faculdade de Ciências Farmacêuticas/Universidade de São Paulo Faculdade de Ciências Farmacêuticas/Universidade de São Paulo, Ribeirão Preto Faculdade de Farmácia/Universidade Federal de Minas Gerais School of Medicine/University of New Mexico, USA Universidade de Sorocaba (UNISO) Institut National de la Recherche Agronomique, France

SCOPE AND POLICY The Brazilian Journal of Pharmaceutical Sciences (BJPS) is a peer-reviewed electronic journal published quarterly by the School of Pharmaceutical Sciences of the University of São Paulo. The purpose of the Brazilian Journal of Pharmaceutical Sciences is to publish manuscripts that significantly contribute to knowledge in all areas of Pharmaceutical Sciences, including Medicines and Drugs, Pharmaceutical and Health Care, Food and Experimental Nutrition, Clinical Chemistry, Toxicology, Medicinal Chemistry, Pharmaceutical Technology, Biotechnology among others. The following papers will not be accepted for publication: • Studies on human subjects not approved by an accredited Ethics Committee or without written informed consent from the subject or legal guardian. • Studies on animals not approved by an accredited Ethics and Animal Care Committee. • Manuscripts describing plant extract activity that do not identify quali and quantitative markers of the extract. PREPARATION OF THE MANUSCRIPTS Manuscripts that do not agree to the Instructions will be refused prior to peer review. Manuscripts must be submitted in English. Submission of a manuscript to BJPS implies that the data have not been published previously and will not be submitted for publication elsewhere while the manuscript is under review. Co-authors should be individuals who have contributed substantially to the content of the paper. Manuscripts in accordance to the “Preparing your manuscript section” will be submitted for peer review to at least two independent, anonymous referees indicated by the Associated Editors. Based on peer review, the Associate Editors will suggest manuscript acceptance or not to the Editor-in-Chief, who is responsible for the final decision. In the case revision is suggested, the authors are asked to resubmit the manuscript incorporating the suggestions and recommendations of the referees within 15 calendar days. If the revised version is not received within the time specified from the date of notice, the manuscript process will be canceled. All revisions must be accompanied with a letter detailing the changes made to the original document and answering all the reviewer comments, on a point-bypoint basis. All alterations must be identified in the revised manuscript. Manuscripts must have their copyright assigned to the BJPS before submitting to the Journal. The dates of receipt and acceptance will be published for each article. Authors are expected to return reviewed manuscripts to the Journal within 15 calendar days, and to return galley proofs of accepted manuscripts within 72 hours. The total number of “late” days will be added to the submission date at the time of publication. Authors are required to suggest 4 potential reviewers with information of institutional and e-mail address. At least 2 of the potential reviewers suggested should be from a different country to the corresponding authors. The Editors reserve the right to indicate these or other reviewers for manuscript evaluation.

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MANUSCRIPT CATEGORIES The authors should state in the cover letter that the manuscript is intended to be Full-length Original Paper, Short Communication, Review Article, Mini-review article, Concepts and Comments and Book Reviews. The Journal will also publish Thematic or Congress Abstracts Supplements under invitation by the Editors or previous approval of the Editorial Board. BJPS will publish the following type of articles: Full-length Original Paper Each manuscript should clearly state its objective or hypothesis; the experimental design and methods used; the essential features of any interventions; the main outcome measures; the main results of the study; and a discussion placing the results in the context of published literature. The manuscript should contain: • abstract of no more than 250 words • no more than 6 key words • a running title to be used as a page heading, which should not exceed 60 letters and spaces • manuscript main body divided into separate sections (Introduction, Material and Methods, Results and Discussion). • no more than 40 references (without exceptions) • Supplementary data can be submitted as Suppmentary information session. Short Communication A short communication is a report on a single subject, which should be concise but definitive. The scope of this section is intended to be wide and to encompass methodology and experimental data on subjects of interest to the readers of the Journal. The manuscript should contain: • abstract of no more than 250 words • no more than 6 key words • a running title to be used as a page heading, which should not exceed 60 letters and spaces • manuscript main body divided into separate sections (Introduction, Material and Methods, Results and Discussion), without a separate section for conclusions • no more than 20 references (without exceptions) • no more than three illustrations (figures and/or tables) Review Article A review article should provide a synthetic and critical analysis of a relevant area and should not be merely a chronological description of the literature. A review article by investigators who have made substantial contributions to a specific area of Pharmaceutical Sciences will be published by invitation of the Editors. However, an outline of a review article may be submitted to the Editors without prior consultation. If it is judged appropriate for the Journal, the author(s) will be invited to prepare the article for peer review. The manuscript should contain: • abstract of no more than 250 words • no more than 6 key words • a running title to be used as a page heading, which should not exceed 60 letters and spaces

• manuscript main body divided into sections with appropriate titles and subtitles • no more than 90 references (without exceptions) Mini-review Article A mini-review is focused on a restricted part of a subject normally covered in a review article. The structure of the mini-review follows the same rules as the review. Concepts and Comments The Concepts and Comments section provides a platform for readers to present ideas, theories and views. The manuscript should contain: • abstract of no more than 250 words • no more than 6 key words • a running title to be used as a page heading, which should not exceed 60 letters and spaces • manuscript main body divided into sections with appropriate titles and subtitles • no more than 40 references (without exceptions) Book Reviews Written by experts indicated by the Editors or written by the authors. PREPARING YOUR MANUSCRIPT Cover Letter It is important that you include a cover letter with your manuscript. Take the time to consider why this manuscript is suitable for publication in the Brazilian Journal of Pharmaceutical Sciences. Why will your paper inspire other members of your field, and how will it drive research forward? Please explain this in your cover letter. The cover letter should also contain the following information: • Title of article. • Name(s) of all author(s). • Information of Corresponding Author (name, full address, telephone number and e-mail). Authorship requirements Only people who directly contributed to the intellectual content of the paper should be listed as authors. All manuscripts must be, submitted, only, by electronic way. The confirmation of submission is sent by email for all the authors, for their agreement. Authors should meet all of the following criteria, thereby taking public responsibility for the content of the paper: • Conceived, planned and carried out the experiments presented in the manuscript or interpreted the data, or both. • Wrote the paper, or reviewed successive versions. • Approved the final version. • Holding positions of administrative leadership, contributing patients, and collecting and assembling data, however important to the research, are not by themselves criteria for authorship. Any person who has made substantial, direct contribution to the work but cannot be considered an author should be cited in the Acknowledgment section, with permission and a description of his/her specific contribution to the research.

Text format • The text of a manuscript can only be accepted as a Microsoft Word file created with MS Word as a “doc”, “docx” or “rtf” document. • Manuscripts should be sent in 30-36 lines, 1,5 spaced, • Each page should contain the page number in the upper righthand corner starting with the title page as page 1. • Report all measurements in Système International, SI (http://physics.nist.gov/cuu/Units) and standard units where applicable • Names of plants, animals and chemicals should be mentioned according to International Rules available. • Names of drugs can follow the International rules (DCI) or current Brazilian rules (DCB) • Trademarks may be mentioned only once in the text (between parenthesis and initial in capital letter) • Do not use abbreviations in the title and limit their use in the abstract and text. • The length of the manuscript and the number of tables and figures must be kept to a minimum. • Ensure that all references are cited in the text. • Generic names must be used for all drugs. Instruments may be referred to by proprietary name; the name and country of the manufacturer should be given in parenthesis. ORGANIZATION OF THE MANUSCRIPT Most articles published in BJPS will be organized into the following sections: • Title, Authors, Abstract, Key words, Running Title, Author for Correspondence and email address • Introduction • Material and Methods • Results and Discussion • Acknowledgments • References • Tables with a descriptive title and footnote legends • Figures with a descriptive title, descriptive legends and uniformity in format Continuous page numbers are required for all pages including figures. There are no specific length restrictions for the overall manuscript or individual sections. However, we urge authors to present and discuss their findings concisely. We recognize that some articles will not be best presented in our research article format. If you have a manuscript that would benefit from a different format, please contact the editors to discuss this further. Title Page Title - The title should be as short and informative as possible, should not contain non-standard acronyms or abbreviations, and should not exceed two printed lines. Examples: Freeze-drying of ampicillin solid lipid nanoparticles using mannitol as cryoprotectant A fully validated microbiological assay for daptomycin injection and comparison to HPLC method Pharmacokinetics, safety and tolerability of L-3-n-butylphthalide

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tablet after single and multiple oral administrations in healthy Chinese volunteers. Authors and Affiliations Full name (matched with superscript numbers identifying affiliation). Institution(s) (Department, Faculty, University, City, State, Country) of each author (in English). Examples: Hongmei Xia1 * , Yongfeng Cheng2 , Yinxiang Xu3 , Zhiqing Cheng1 1 College of Pharmacy, Anhui University of Chinese Medicine, Hefei, People’s Republic of China. 2 School of Life Science, University of Science and Technology of China, Hefei, People’s Republic of China. 3 Zhaoke (Hefei) Pharmaceutical Co. Ltd., Hefei, People’s Republic of China. Abstract Since abstracts are published separately by Information Services, they should contain sufficient hard data to be appreciated by the reader. The abstract should not exceed 250 words and should be prepared in a single paragraph. The abstract should briefly and clearly present the objective, experimental approach, new results as quantitative data if possible, and conclusions. It should mention the techniques used without going into methodological detail and mention the most important results. Abbreviations should be kept to a minimum and should be defined in both the Abstract and text. Please do not include any reference citations in the abstract. If the use of a reference is unavoidable, the full citation should be given within the abstract. Key Words A list of key words or indexing terms (no more than 6) should be included avoiding generic terms. Running title This short title, to be used as a page heading, should not exceed 60 letters and spaces. Corresponding author One of the authors should be designated as the corresponding author. It is the corresponding author´s responsibility to ensure that the author list is accurate and complete. If the article has been submitted on behalf of a consortium, all consortium members and affiliations should be listed in the Acknowledgments section. Provide the name and email address of the author to whom correspondence should be sent identified with an asterisk. Introduction The Introduction should put the focus of the manuscript into a broader context and reflects the present state-of-art of the subject. This should state briefly and clearly the objectives of the investigation with reference to previous works. Extensive review of the literature should be avoided and substituted for references of recent review publications. Material and Methods These should be described in sufficient detail that the work can be

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reproduced. Well-established procedures and techniques require only a citation of the original source, except when they are substantially modified. Reports of experimental studies on humans and animals must certify that the research received prior approval by the appropriate institutional review Ethics Committee. Results and Discussion Results must be presented clearly and concisely and in logical order. This section should provide results of all of experiments required to support the conclusions of the paper. When possible, use figures or tables to present data rather than text. Large datasets, including raw data, should be submitted as supplementary files; these are published online linked to the article. Discussion should interpret the results and assess their significance in relation to existing knowledge. Speculation not warranted by actual data should be avoided. The Discussion should spell out the major conclusions and interpretations of the work including some explanation of the significance of these conclusions. Acknowledgments When appropriate, briefly acknowledge technical assistance, advice and contributions from colleagues. People who contributed to the work but do not fit the criteria for authors should be listed in the Acknowledgments section, along with their contributions. Donations of animals, cells, or reagents should also be acknowledged. You must also ensure that anyone named in the Acknowledgments agrees to being so named. Financial support for the research and fellowships should be acknowledged in this section (agency and grant number). Figures Figures must be submitted in high-resolution version (600 dpi). Preparing figure files for submission BJPS encourages authors to use figures where this will increase the clarity of an article. The use of color figures in articles is free of charge. The following guidelines must be observed when preparing figures. Failure to do so is likely to delay acceptance and publication of the article. • Each figure of a manuscript should be submitted as a single file. • Figures should be numbered in the order they are first mentioned in the text, and uploaded in this order. • Figure titles and legends should be provided in the main manuscript as a List of Figures, not in the graphic file. • The aim of the figure legend should be to describe the key messages of the figure, but the figure should also be discussed in the text. • An enlarged version of the figure and its full legend will often be viewed in a separate window online, and it should be possible for a reader to understand the figure without moving back and forth between this window and the relevant parts of the text. • The legend itself should be succinct, while still explaining all symbols and abbreviations. Avoid lengthy descriptions of methods. Statistical information should be given as well as the statistical tests used. • Arrows or letters should be used in the figure and explained in the legend to identify important structures.

• Figures with multiple panels should use capital letters A, B, C, etc. to identify the panels. • Each figure should be closely cropped to minimize the amount of white space surrounding the illustration. Cropping figures improves accuracy when placing the figure in combination with other elements, when the accepted manuscript is prepared for publication. • Individual figure files should not exceed 5 MB. If a suitable format is chosen, this file size is adequate for extremely high quality figures. Please note that it is the responsibility of the author(s) to obtain permission from the copyright holder to reproduce figures (or tables) that have previously been published elsewhere. In order for all figures to be open-access, authors must have permission from the rights holder if they wish to include images that have been published elsewhere in non-open-access journals. Permission should be indicated in the figure legend, and the original source included in the reference list. Supported file type The following file format can be accepted: TIFF (suitable for images) or JPEG with 600 dpi, and Word file for the manuscript. Tables • Tables must be submitted in Word (.doc) or Excel (.xls), not as an image. • Tables must be numbered consecutively with Roman numerals in the text. • Tables must have a concise and descriptive title. • All explanatory information should be given in a footnote below the table. Footnotes should be used to explain abbreviations and provide statistical information, including statistical tests used. • All abbreviations must be defined in this footnote, even if they are explained in the text. • Tables must be understandable without referring to the text. • Tables occupying more than one printed page should be avoided, if possible. • Vertical and diagonal lines should not be used in tables; instead, indentation and vertical or horizontal space should be used to group data. References References should be prepared and listed according to Vancouver standard reference style. Entries should be arranged in alphabetical order by author at the end of the paper. All authors’ names should be given. Accuracy and completeness of reference data is the responsibility of the authors. Only published references should be included in the reference list. Meeting abstracts, conference talks, or papers that have been submitted but not yet accepted should not be cited. Limited citation of unpublished work should be included in the body of the text only. All personal communications should be supported by a letter from the relevant authors. References should be cited in the text by the authors’ names, with only the first letter in capital letter followed by the year of publication. For more than three authors, the first has to be cited followed by the expression et al. (in italic). Small letters close to the year must differentiate references of the same authors and year of publication.

Examples: (Fujisawa, Atsumi, Kadoma, 1989) (Aviral et al., 2009) (Dodu, Rotari, Vazques, 2012) (Liu et al., 2011a) (Liu et al., 2011b) Please use the following style for the reference list: Published Papers. First 6 authors followed by et al., Title, Journal (abbreviation in italic), Year, Volume, Complete Pages. Abe T, Fukushima N, Brune K, Boehm C, Sato N, Matsubayashi H, et al. Genome‐Wide allelotypes of familial pancreatic adenocarcinomas and familial and sporadic intraductal papillary muninous neoplasms. Clin Cancer Res. 2007;13(20):6019‐25.

Ali A, Iqbal F, Taj A, Iqbal Z, Amin MJ, Iqbal QZ. Prevalence of microvascular complications in newly diagnosed patients with Type 2 diabetes. Pak J Med Sci. 2013,29(4): 899-902.

Calvo A, Gimenez MJ. Ex Vivo Serum Activity (Killing Rates) After Gemifloxacin 320 mg Versus Trovafloxacin 200 mg Single Doses Against Ciprofloxacin-Susceptible and -Resistant Streptococcus pneumoniae. Int J Antimicr Ag. 2007;20:144-6.

Lammers AE, Hislop AA, Flynn Y, Haworth SG. The 6-minute walk test: normal values for children of 4-11 years of age. Arch Dis Child. 2008;93:464-468.

Zhang Q, Malik P, Pandey D, Gupta S, Jagnandan D, Belin de CE, et al. Paradoxical activation of endothelial nitric oxide synthase by NADPH oxidase. Arterioscler Thromb Vasc Biol. 2008;28:1627-1633.

Article accepted for publication but not yet published. First 6 authors followed by et al., Title, Journal (abbreviation in italic), Year of expected publication, (in press) at the end of the citation. Janiszewski M, Lopes LR, Carmo AO, Pedro MA, Brandes RP, Santos CXC, et al. Regulation of NAD(P)H oxidase by associated protein disulfide isomerase in vascular smooth muscle cells. J Biol Chem. 2005 (in press). Internet Communication. Ensure that URLs are active and available. Provide DOI, if available. Brasil. Ministério da Saúde, Secretaria de Vigilância em Saúde. Leishmaniose visceral grave: normas e condutas [Internet]. Brasília (DF): Ministério da Saúde, 2006. [citado 2008 Jan 7]. 60 p. (Série A. Normas e Manuais Técnicos). Disponível em: http://dtr2001.saude.gov.br/editora/produtos/livros/ pdf/06_0072_M.pdf

CAPES Statistics. [citad 2006 Mar 16]. Available from: http:// www.capes.gov.br/capes/portal.

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Book, Whole. Authors, Book title, Edition, City, Publisher, Year. Hewitt W. Microbiological assay for pharmaceutical analysis: a rational approach. Boca Raton: CRC Press; 2003.

Jenkins PF. Making sense of the chest x-ray: a hands-on guide. New York: Oxford University Press; 2005. 194 p.

Milech A, et al., Oliveira JEP, Vencio S, organizadores. Diretrizes da Sociedade Brasileira de Diabetes. São Paulo: A.C. Farmacêutica;2016.

Book, Chapter. Authors, Chapter Title, Editors, Book title, Edition, City, Publisher, Year, Pages of citation.

Beizer JL, Timiras ML. Pharmacology and drug management in the elderly. In: Timiras PS, editor. Physiological basis of aging and geriatrics. 2nd ed. Boca Raton: CRC Press; 1994. p. 279-84.

Rojko JL, Hardy WD Jr. Feline leukemia virus and other retroviruses. In: Sherding RG, editor. The cat: diseases and clinical management. New York: Churchill Livingstone; 1989. p. 229-332.

Report World Health Organization. WHO. Department of Mental Health and Substance Abuse. Mental health atlas 2005. Geneva: World Health Organization; 2005. 409 p.

Laws Agência Nacional de Vigilância Sanitária (Brasil). Resolução nº. 259, de 20 de setembro de 2002. Regulamento Técnico para Rotulagem de Alimentos Embalados. Diário Oficial da União 23 set 2002; Seção 1. Conference, Symposium Proceedings. Cite papers only from published proceedings. Hejzlar RM, Diogo PA. The use of water quality modelling for optimizing operation of a drinking water reservoir. In: Proceedings of the International Conference Fluid Mechanics and Hydrology. 1999 Jun 23-26; Prague. Prague: Institute of Hydrodynamics AS CR; 1999. p 475-482.

Proceedings of the 10th annual meeting of the Canadian Society for Pharmaceutical Sciences. J Pharm Pharm Sci. 2007 Dec 3;10(4):1s-186s.

Audiovisual Material Physician’s Desk Reference (PDR). Release 2003.1AX. [CDROM]. Montvale: Thomson PDR; 2003. Computer Program Dean AG, Dean JA, Coulombier D, Brendel KA, Smith DC, Burton AH, et al. Epi info, version 6.04: a word processing database and statistics program for public health on IBMcompatible microcomputers. [Computer program]. Atlanta: Centers of Disease Control and Prevention; 1998.

World Health Organization. WHO. Working to overcome the global impact of neglected tropical diseases, First WHO report on neglected tropical diseases. Geneva, Switzerland: WHO Press; 2010.

Patent Larsen CE, Trip R, Johnson CR. Methods for procedures related to the electrophysiology of the heart. Patent No. 5.529.067. Novoste Corporation; 1995.

Thesis and Dissertations Joselevitch C. Visão no ultravioleta em Carassius auratus (Ostariophysi, Cypriformes, Cyprinidae): estudo eletrofisiológico do sistema cone - células horizontais. [Master’s dissertation]. São Paulo: Instituto de Psicologia, USP; 1999.

“Unpublished results” and “Personal communication”. Reference should appear in the text with the individual name(s) and initials and not in the reference list. (Santos CS, da-Silva GB, Martins LT, unpublished results). It is assumed that the author has obtained permission from the source when “personal communication” is cited.

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