Typical Classroom Results The data below represent typical classroom results for experiments run at 22°C and using a SmartSpec Plus spectrophotometer for quantitative analysis. However, please note that the enzyme used in this kit is highly temperature dependent. Because of this, changes in room temperature can have an effect on the rate of reaction relative to the results depicted. An increase in ambient room temperature will increase the reaction rate and can lead to saturation of absorbance at an earlier reaction time point. Conversely, a colder room temperature will lead to slower reaction rates. The trends depicted in the typical classroom data should be consistent irrespective of the exact temperature at which they were run. Activity 1: Determine the Reaction Rate in the Presence or Absence of an Enzyme Qualitative data Time
Cuvette
(minutes)
0 8 1 2 4 6 8
Start End E1 E2 E3 E4 E5
Standard That Is Most Similar S1 S1 S2 S3 S4 S5 S5
Quantitative data Standard
INSTRUCTOR'S MANUAL TYPICAL RESULTS
S1 S2 S3 S4 S5
Amount of p-Nitrophenol (nmol) 0 12.5 25 50 100
Absorbance at 410 nm 0.00 0.21 0.42 0.82 1.65
Instructor’s Manual
18
Amount of p-Nitrophenol (nmol) 0 0 12.5 25 50 100 100