Resultados tipicos

Page 1

Typical Classroom Results The data below represent typical classroom results for experiments run at 22°C and using a SmartSpec Plus spectrophotometer for quantitative analysis. However, please note that the enzyme used in this kit is highly temperature dependent. Because of this, changes in room temperature can have an effect on the rate of reaction relative to the results depicted. An increase in ambient room temperature will increase the reaction rate and can lead to saturation of absorbance at an earlier reaction time point. Conversely, a colder room temperature will lead to slower reaction rates. The trends depicted in the typical classroom data should be consistent irrespective of the exact temperature at which they were run. Activity 1: Determine the Reaction Rate in the Presence or Absence of an Enzyme Qualitative data Time

Cuvette

(minutes)

0 8 1 2 4 6 8

Start End E1 E2 E3 E4 E5

Standard That Is Most Similar S1 S1 S2 S3 S4 S5 S5

Quantitative data Standard

INSTRUCTOR'S MANUAL TYPICAL RESULTS

S1 S2 S3 S4 S5

Amount of p-Nitrophenol (nmol) 0 12.5 25 50 100

Absorbance at 410 nm 0.00 0.21 0.42 0.82 1.65

Instructor’s Manual

18

Amount of p-Nitrophenol (nmol) 0 0 12.5 25 50 100 100


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