Mote 50th Anniversary Annual Report

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MOTE MARINE LABORA LABORATORY

SHARK RESEARCH

Robert E. Hueter, Ph.D., Director HISTORICAL HIGHLIGHTS

1955 The Cape Haze Marine Lab, the precursor to Mote Marine Laboratory, is created by Dr. Eugenie Clark. Studies begin on shark distribution, reproduction and behavior.

1959 Dr. Clark performs the first conditioning experiments with sharks, showing that they can learn.

1967 New studies on shark physiology and anti-shark methods begin under Dr. Perry Gilbert.

1974 Dr. David Baldridge finishes the first computerized statistical analysis of shark attack data in the International Shark Attack File.

1979 Marine biomedical studies focusing on elasmobranch cancer resistance begin.

1988 New environmental and conservation biology studies begin as sharks are threatened by overfishing.

1991 The Center for Shark Research is established and designated by Congress as a national research center.

Making Sense of Sharks Whether it was discovering what sharks do in hurricanes, the secrets they hold for potential cancer therapies or finding out where the sea’s biggest fish gather, 2004 shed new light on these fascinating animals. • By separating the proteins produced

Atlantic stingrays in the St. Johns River.

by shark immune cells, biomedical and

Research is ongoing to understand

immunology research scientists opened

hormonal regulation in this and other

the door to identifying exactly what it is

shark species.

in sharks that can be used to inhibit the

• Construction of a new research seawater

growth of human tumor cells. This is a

system with mega-tanks greatly enhanced

vital step toward discovering potential

the ability of Mote’s shark center scientists

therapeutic applications for the treatment

to conduct experimental studies of sharks

of cancer in humans.

in a laboratory setting. Thanks to the larger

• New data showed that sharks sense

tanks, great hammerhead sharks have

barometric pressure and use this sense as

been kept in excellent health for the first

a survival tactic to flee shallow areas when

time in any research facility.

tropical storms and hurricanes approach.

• The center hosted the Shark Specialist

• Mote shark biologists revealed one of the

Group of IUCN-World Conservation, which

world’s largest gatherings of whale sharks,

develops the world’s most comprehensive

which feed by the hundreds off Mexico’s

inventory of the global status of plant

Yucatan peninsula. Studies are under way

and animal species. The international

on this aggregation of the biggest fish in

group convened at Mote to determine

the sea.

the population status of sharks and rays

• Ongoing studies of the reproductive

in North and Central America, finding

physiology of live-bearing sharks and rays

that some species have become critically

showed that environmental pesticides

endangered. With strict management,

were not the cause of reproductive

other species in a similar situation have

problems in a freshwater population of

recovered from their depleted status.

1998 Polly Loomis Endowment for Marine Biomedical Research established.

2001 Perry W. Gilbert Endowed Chair in Shark Research established.

2002 Mote coordinates the creation of the National Shark Research Consortium. Other members are the University of Florida, Moss Landing Marine Laboratories and the Virginia Institute of Marine Science. 2004 Annual Report

PROGRAMS & MANAGERS ELASMOBRANCH BEHAVIORAL ECOLOGY, Michelle Heupel, Ph.D. Examines shark movement patterns and shark habitats in coastal estuaries and rivers. ELASMOBRANCH FISHERIES & CONSERVATION BIOLOGY, Colin Simpfendorfer, Ph.D. Assesses the population and critical habitat for the endangered smalltooth sawfish and commercial shark species and assesses ecosystem models for sharks and rays. ELASMOBRANCH PHYSIOLOGY & ENVIRONMENTAL BIOLOGY, James Gelsleichter, Ph.D. Studies the reproductive physiology of live-bearing sharks and rays. MARINE BIOMEDICAL RESEARCH, Carl Luer, Ph.D. Studies disease resistance, biochemistry and embryonic development of sharks, skates and rays. MARINE IMMUNOLOGY, Cathy Walsh, Ph.D. Characterizes cellular immune function in elasmobranchs, identifies immune regulatory factors, and characterizes environmental stressors on manatee health. SHARK BIOLOGY, Robert E. Hueter, Ph.D. Studies shark abundance, nursery grounds, fisheries impacts, shark feeding mechanisms and shark sensory systems.


MISSION: Mote’s Center for Shark Research is the largest shark research center in the world. Designated as a national center by the U.S. Congress in 1991, it is dedicated to the scientiďŹ c study of sharks, skates and rays and conducts laboratory and ďŹ eld research on these animals from the molecular biology level in the lab to shark populations in the sea.


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MOTE MARINE LABORA LABORATORY

COASTAL ECOLOGY COAST

Ernest D. Estevez, Ph.D., Director HISTORICAL HIGHLIGHTS

1966 Pioneering algae studies by Sylvia Earle on Florida’s west coast begin.

1967 First ecological studies of Charlotte Harbor’s sediments, salinity and fishes begin.

1971 First Charlotte Harbor field station established at Placida.

1972 Selby Environmental Health Fellowship established.

Coastal Discovery Using advanced technology and new methodologies, center scientists explored the sea as never before, finding new methods to track pollution and raise shellfish and exploring caverns hundreds of feet below the surface of the Gulf of Mexico. • Working with independent divers, Mote

• Staff succeeded in using innovative

staff found new underwater caverns,

methods to stock the once common and

possibly the world’s largest, in the Gulf

always prized bay scallop in the waters of

of Mexico off Sarasota. These sinkholes

Charlotte Harbor.

1975 Estuarine Ecology

and caves offer great promise for

• Other innovative research included

and Environmental Health research programs created at Mote.

archaeological, historical, geological and

identifying grouper habitat off Florida’s

ecological discoveries.

west coast and in the Keys using a side

• Staff developed, tested and used a new

scan sonar system to “paint” an image of

method for recognizing optical brighteners

the sea floor.

Assessment Division is established at Mote.

– a laundry detergent additive designed

• The center played a key role as

to make clothes brighter – in natural

coordinator of the Charlotte Harbor

1982 First studies of

waters. This powerful new tool could

Research Project, which involved other

Florida rivers begin.

help demonstrate septic tank pollution in

Mote science centers, during a time of

1984 First modern

natural waterways.

upheaval. As the year began, studies

study of invertebrates living in Charlotte Harbor begins.

• New research in 2004 brings to more

sought to confirm that harbor ecology

than three dozen the number of Florida

would resemble that of 2003, a very

rivers studied by center scientists. Their

wet year. But Hurricane Charley and

Scientific Diving Program established within the center.

work studying the state’s tidal rivers – more

subsequent hurricanes made 2004 even

than any other institution – was recognized

wetter, bringing unexpected consequences

during a worldwide rivers symposium in

to the harbor’s ecology. Post-hurricane

1991 Center’s first

Australia. One finding was that a type of

studies were more difficult due to the

vegetation was not flourishing as expected

destruction of the Mote field station on

in the Caloosahatchee River. The search

Pine Island. Nevertheless, by the October

for reasons included a study of the species

start of the annual Charlotte Harbor

1994 Center provides

at Mote laboratory in Sarasota. The work is

Conference at Mote, scientists were able to

the first international training workshop at Mote.

important because the river’s recovery is

report on the storm’s initial impacts to the

a necessary part of the Florida Everglades

harbor’s marine life and habitat.

1974 First ecological studies of Sarasota Bay and Venice waters.

1979 Environmental

1990 AAUS-approved

international research trip on the southern coast of India takes place.

1998 Modern Center for Coastal Ecology is established at Mote.

2000 Landscape Ecology Program created; second Charlotte Harbor Field Station established at Pineland.

2001 Charlotte Harbor Research Project begins.

2004 Annual Report

restoration.

PROGRAMS & MANAGERS BENTHIC ECOLOGY, James Culter, Staff Scientist. Explores life in the bottom sediments of Florida’s rivers, bays and estuaries and conducts nationally significant studies at electric power stations. CHEMICAL ECOLOGY, L. Kellie Dixon, Ph.D. candidate. Monitors coastal water quality in Sarasota County and Charlotte Harbor and is working to advance the study of marine optics. COASTAL RESOURCES, Ernest D. Estevez, Ph.D. Developing methods and applications to use mollusks as markers to portray ecological conditions in tidal rivers and coordinates the Charlotte Harbor Research Program. LANDSCAPE ECOLOGY, Brad D. Robbins, Staff Scientist. Working to understand wild celery grass ecology in the Caloosahatchee River and using Geographic Information Systems for very large-scale ecological studies.


MISSION: Mote’s Center for Coastal Ecology studies the relationship between organisms and their environments in Florida’s coastal waters – their past, present and future – in order to develop scientific methods to restore and protect these great marine resources.


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MOTE MARINE LABORA LABORATORY

MARINE MAMMAL & SEA TURTLE RESEARCH Randall S. Wells, Ph.D., Director HISTORICAL HIGHLIGHTS

1959 Rare beaked whale remains are donated by Mote to the American Museum of Natural History.

1970 Office of Naval Research funds studies on the interactions between dolphins and sharks; dolphin tagging studies begin from Tampa Bay to Charlotte Harbor, documenting these animals’ local residency for the first time. The program continues today as the world’s longest-running dolphin research program.

Toxins Taken to Task The center made new strides in understanding marine mammal and sea turtle biology and behavior in 2004 with studies looking at everything from toxin exposures to the discovery of new species and learning more about where sea turtles spend their lives in the water • One interesting development was the

• New in-water studies in Charlotte

discovery of a fatty acid biomarker that

Harbor yielded 160 turtle locations for

indicates exposure to red tide toxin and

three species: loggerhead, Kemp’s ridley

other health-stressing agents in manatees

and green turtles. Earthwatch began

1982 Genie, a second

and, perhaps, other marine animals.

supporting the study in July, but Hurricane

generation in Mote’s wild dolphin study, gives birth to the study’s third generation.

• Special projects began to address Arctic

Charley destroyed Mote’s Charlotte Harbor

marine mammal conservation and assess

Field Station and delayed the study’s

health risks to native peoples who are

progress.

1982 Sea turtle nest

subsistence hunters.

• Three years of data from instruments

monitoring begins.

• High levels of first-born bottlenose

deployed in Charlotte Harbor show that

1984 Mote standardizes

dolphin calf mortality were found to be

the acoustic behavior of marine life can

its response to stranded marine mammals.

correlated with the concentrations of

be correlated with many environmental

environmental contaminants, such as

changes and can be used as a measure of

1985 First aerial survey

PCBs, found in the mothers’ milk. The

biological activity. While Hurricane Charley

contaminants are being transferred

passed directly over Charlotte Harbor, it

through nursing.

did not affect fish spawning.

• Collaborative research produced papers

• New manatee studies found that

on the discovery of a new whale species

manatee facial hairs, or vibrasse, can

as well as new internal and external whale

sense different surface textures at a level

parasites.

at least equal to the human touch.

• The turtle program coordinated more

• Staff entered the 35th year of studies of

than 140 volunteers and eight summer

four generations of dolphins in Sarasota

interns to monitor Longboat, Siesta, and

Bay. Research examines the ecology,

Casey keys and the Venice beaches in

behavior and health of dolphins as well as

Sarasota County and documented 875

the impacts of human activities.

false crawls and 797 nests – including two

• Staff worked on many peer-reviewed

green turtle nests. This marked the lowest

manuscripts and another book.

of manatees.

1987 Sarasota County adopts sea turtle protection plan because of Mote’s efforts.

1990 Bottlenose dolphins Misha and Echo are released in Tampa Bay, marking the first scientific effort to monitor captive dolphins after their return to native waters. 1994 The Ann and Alfred Goldstein Marine Mammal Center opens and the Fran Warren Endowment for Marine Mammals is established.

1996 Genie’s daughter Claire gives birth to the first documented fourth generation of Sarasota Bay dolphins.

1998 The Center for Marine Mammal and Sea Turtle Research is officially formed.

2004 Annual Report

nesting year for Sarasota and the U.S. since 1991.

PROGRAMS & MANAGERS DOLPHIN RESEARCH, Dr. Randall Wells, Ph.D. Studies coastal dolphins to understand the population dynamics and structure of small cetaceans and the human factors that affect them. The work is done in collaboration with the Chicago Zoological Society and colleagues worldwide. Offshore dolphin studies seek to understand deepwater dolphin species from a genetic to a population level. MANATEE RESEARCH, John Reynolds III, Ph.D. Partners with other organizations to answer important questions about manatee biology, health and behavior to understand the species and help inform management decisions and educate the public. SEA TURTLE CONSERVATION AND RESEARCH, Jerris Foote, Staff Scientist. Coordinates turtle nest monitoring activities in Sarasota County to aid in the assessment of suitable nesting habitats. In-water studies seek to determine sea turtle abundance and habitat usage in Charlotte Harbor to aid management decisions that may affect juvenile sea turtles. SENSORY BIOLOGY AND BEHAVIOR, William Tavolga, Ph.D. Studies the sensory and cognitive processes in a variety of marine mammals. STRANDING INVESTIGATIONS, Nélio Barros, Ph.D. Provides 24-hour response to marine mammal and sea turtle strandings in coastal Southwest Florida and offers logistical stranding support to state manatee biologists. Studies seek to understand the natural history of cetaceans and sea turtles and evaluate long-term mortality trends.


MISSION: Mote’s Center for Marine Mammal and Sea Turtle Research provides the highest quality information to enhance the understanding of the biology, and habitat requirements of these aquatic vertebrates to inform conservation and management decisions. The center also provides professional leadership among peers and students worldwide and disseminates ďŹ ndings to scientists, conservationists and the general public.


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MOTE MARINE LABORA LABORATORY

ECOTOXICOLOGY

Richard H. Pierce, Ph.D., Director HISTORICAL HIGHLIGHTS

1957 The first samples of the red tide organism are displayed at the lab.

1962 Mote launches its first study of red tide. 1973 Mote, NASA and the state of Florida show that red tide blooms begin offshore. 1981 The Chemical Fate and Effects Program begins as the precursor to the Center for Ecotoxicology.

Red Tide Response Measuring human and animal reactions to red tide and chemical contaminants and finding new ways to assess exposures were key questions addressed in 2004. New systems were also designed to search for red tide using unmanned vehicles and stationary BreveBusters. • Research found a new sequence of

red tide bloom dynamics. T Tests have

fatty acid compounds that appear to be

revealed how some red tides form along

produced in manatees exposed to red tide

the bottom and create bloom conditions

toxins. This “biomarker fingerprinting” is

when upwellings occur. These preliminary

an important forensic tool for assessing the

studies produced the groundwork for

cause of manatee illness or death. New

Mote’s Sarasota Operations Coastal

studies are under way to evaluate similar

Oceans Observation Laboratory (SO COOL)

“fingerprints” in dolphins and whales.

to be installed in 2005 as a control station

• A compound produced by the red tide

for observing red tide, opening the door for

organism, Karenia brevis, that inhibits its

other research opportunities.

own toxicity has been found in a red tide

• Work began to create new tests to

bloom along the Sarasota Gulf coast. The

determine how red tide toxins break

compound was initially identified in a lab

down in shellfish tissues with the goal

culture at the University of North Carolina,

of producing more exacting and timely

Ecotoxicology established; Mote partners with S.T.A.R.T. and Woods Hole to investigate red tide mitigation.

Wilmington. The so-called “antagonist”

testing for shellfish bed closures during

could be used to reduce red tide impacts

red tides. The studies are also designed

on humans and marine mammals.

to assess how toxic compounds are

• New research showed the human

transferred to marine animals that eat

impact of red tides. People with chronic

shellfish.

1999 The Sylvia and

respiratory disease are affected by even

• Coral health studies in the new Marine

Mel Levi Endowed Chair in Ecotoxicology established.

small doses of airborne red tide toxins,

Microbiology Program are focusing on

whereas healthy individuals exhibit

bacteria and fungi that promote a healthy

irritation and discomfort but show no

environment for coral and fend off disease.

respiratory dysfunction during and after

Studies to provide baseline information

exposure.

about microorganisms associated with

• New BreveBuster red tide sensors have

healthy and diseased coral are under way

been deployed in autonomous underwater

with NOAA and the Florida Keys National

vehicles and on channel markers for

Marine Sanctuary.

1984 Research with liquid chromatograph technology begins to detect toxins in water, air and animal tissue.

1987 Achieved a breakthrough in hazard assessment of pesticide impacts and provides the first characterization of airborne red tide toxins that affect humans. 1992 BreveBuster development begins.

1998 Center for

2000 Research begins on red tides and human health and chemical contaminant projects begin in the Arctic.

2001 Obtained advanced technology to identify individual harmful algae compounds and the first sea trials of the BreveBuster begin.

2003 Initiated studies of neurotoxic shellfish poisoning in Sarasota Bay clams and oysters.

2004 Annual Report

continuous, real-time data regarding

PROGRAMS & MANAGERS AQUATIC TOXICOLOGY, Dana Wetzel, Ph.D. Assesses petroleum residues and pesticides in marine mammals eaten by native subsistence hunters and looks for biomarkers that could assess other toxins in other groups of marine mammals. CHEMICAL FATE AND EFFECTS, Michael Henry, Staff Scientist. Studies seek to discover how natural toxins and chemical pollutants are distributed, persist and break down in the marine environment. ENVIRONMENTAL HEALTH, Barbara Kirkpatrick, Ed.D. Studies how humans are affected by aerosolized red tide toxins and how to effectively educate residents and visitors about those studies. PHYTOPLANKTON ECOLOGY, Gary Kirkpatrick, Ph.D. Current work addresses phytoplankton behavior, photophysiology and bloom dynamics, especially for red tide. Lab studies seek to understand how harmful algae function at cellular, community and ecosystem levels. MARINE MICROBIOLOGY, Kimberly Ritchie, Ph.D. Seeks to establish health assessments for Florida coral reef tract and for microbial baselines of coral communities.


MISSION: Mote’s Center for Ecotoxicology investigates the sources of natural biotoxins and chemical pollutants and how they break down in the marine environment. The center also assesses the risks such toxins pose to public health and natural resources and seeks new techniques to monitor and reduce those affects. These studies involve marine biology, ecology, chemistry, toxicology, microbiology and health sciences.


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MOTE MARINE LABORA LABORATORY

FISHERIES ENHANCEMENT Kenneth M. Leber, Ph.D., Director HISTORICAL HIGHLIGHTS

1979 First comprehensive survey of fish populations in Sarasota Bay begins. 1985 Mote and the Florida Fish and Wildlife Research Institute create a partnership to scientifically investigate restoration of depleted marine and estuarine fisheries. Today the partnership has major influence on restoration efforts throughout the U.S.

Sustaining Fish Populations Sustaining fisheries that are ecologically and economically important to Florida is an important goal in the center. Key components include finding ways to scientifically evaluate stock enhancement procedures and improve their effectiveness and viability. • Studies documented that hatchery-

alterations related to development, the

reared snook can double the size of a

center investigated fish populations in

snook population without displacing the

brackish streams and showed that fish

wild fish. A 34-inch-long hatchery snook

favored the restored streams. Studies

was recaptured in 2004, five years after

also included the importance of habitat

being released as a 5-inch juvenile,

connectivity to snook, red drum and

showing that stocked snook can survive to

tarpon.

spawning size.

• As part of the Science Consortium for

• A new adult snook movement and

Ocean Replenishment, the center worked

1995 Charles M. Breder,

mortality study began in Sarasota Bay

to develop marine stock enhancement

Jr. Chair established to support the study of the biology, behavior and conservation of fishes.

using legal-size snook implanted with

technology to provide U.S. agencies with

acoustic tags. The snook were tracked

an effective, turnkey tool for restoring

using boats with remote receivers, and

depleted populations of valuable fishes.

results will be used in stock assessment.

• In Project T Tampa Bay, Mote scientists

Mote International Symposium in Fisheries Ecology is held.

• Geographic studies compared the age,

joined state biologists to plan, execute and

growth, reproduction, movement and

analyze one of the largest experimental

survival of red snapper in Florida waters.

releases of lab-cultured red drum ever.

1997 First experimental

The center also evaluated minimum size

• Research continued to develop methods

regulations for selected reef fish and led a

to boost the survival rate of hatchery-

cooperative long-line sampling of Florida’s

raised red snapper. Studies on the long-

1994 The William R. and Lenore Mote Endowment in Fisheries Ecology and Enhancement established at Florida State University as a Mote-Florida State University partnership.

1996 The first FSU-

release of common snook to evaluate survival patterns of hatchery-reared fish.

west coast shallow water grouper to

spined sea urchin, whose consumption

1998 The Center for

characterize life history, undersize bycatch

of algae promotes coral settlement,

Fisheries Enhancement officially established.

and targeted habitats. New life history and

expanded.

habitat use studies of reef fish began with

• Studies in the Apalachicola drainage

volunteers in the Florida Keys.

basin continued to determine the potential

• In Charlotte Harbor, where the coastline

impact of reduced spawning habitat on the

has undergone decades of drainage

endangered Gulf sturgeon.

2000 Scientists show that hatchery-raised and released snook make up 4 percent of Sarasota Bay’s snook population and that hatchery-reared red drum established a small fishery in Biscayne Bay.

2002 Mote proves the importance of tidal creeks as key snook nursery grounds.

2003 Scientists find that an exotic fish species has invaded Charlotte Harbor. 2004 Annual Report

PROGRAMS & MANAGERS

FISH BIOLOGY, Karen Burns, Ph.D. candidate. Works to understand the life history and migratory patterns of coastal pelagic and reef fishes as the foundation for stock assessment management. FISHERIES ASSESSMENT AND ECOSYSTEM MANAGEMENT, Carl Walters, Ph.D., William and Lenore Mote Eminent Scholar in Fisheries Ecology. Addresses the need for reliable stock assessment since resource management policies must be specified and justified on quantitative terms. FISHERIES HABITAT ECOLOGY, Aaron Adams, Ph.D. Advances knowledge about how to maintain the integrity of fish habitats, including threshold distances between habitats, the need for dispersal corridors, the role of micro-habitat for juveniles, and understanding which species most benefit from an increase in habitat. MARINE STOCK ENHANCEMENT, Ken Leber, Ph.D. Focuses on effective marine stock enhancement technology to restore depleted populations, augment fishery yields in recruitment-limited or habitat-limited stocks, advances basic knowledge about wild stocks, establishes new fisheries on artificial reefs and develops effective strategies for using hatcheries to help conserve coral reef communities.


MISSION: Mote’s Center for Fisheries Enhancement strives to substantially increase the knowledge of how to preserve and responsibly enhance coastal fish and invertebrate populations. The center aspires to be recognized as a leader in fisheries ecology, marine fisheries enhancement and fisheries management strategies through innovative research, training, and promotion of sustainable fishing practices.


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MOTE MARINE LABORA LABORATORY

TROPICAL RESEARCH LABORA LABORATORY/CORAL REEF RESEARCH Bruce Frerer, Interim Executive Director • Erich Bartels, Interim Director HISTORICAL HIGHLIGHTS

1993 Tropical coral reef research station established on Pigeon Key.

1995 Coral reef research program established in the Florida Keys; hard coral propagation studies begins.

1996 Mote establishes proof of calcium channels in coral calcification.

Coral Research and Discovery The Tropical Research Laboratory provided the facilities and the operations center for the Center for Coral Reef Research as well as field headquarters for other Mote research centers working with sharks, marine mammals, red tide, fisheries enhancement and aquaculture. • Work was initiated on a new Coral

laboratory exposure experiments.

EPA initiate the annual Florida Keys Coral Disease Survey.

Bleaching Early Warning Network to help

• Extensive upgrades were made to the

managers detect coral bleaching events

coral culture and coral gene bank facilities

and work to try to prevent corals from

at the Tropical Research Laboratory.

2000 Center for Tropical Research established on Summerland Key; researchers identify molecular biomarkers of coral stress.

dying. The project will use existing remote

The Center for Aquaculture Research

sensing and in-situ monitoring stations

and Development studied new coral

combined with a dedicated network of

growing methods and transplantation

trained volunteer observers to monitor

and established an in-situ nursery for

coral reefs for signs of bleaching. Work

coral fragments rescued from a vessel

also continued on the Ultraviolet Light

grounding. T Twenty-three species of hard

monitoring program, which is designed to

corals are now being grown at the lab.

evaluate the interactions between climate

• Work also continued on the Looe

and UV penetration in the water and

Key telepresence project to station a

its contribution to coral bleaching. The

camera on the Looe Key Reef with live

program determined field measurements

feeds transmitted to the lab for internet

for changes in light penetration over the

dissemination on a real-time basis.

reefs and the effects of water from Florida

• In Key West, the Conch Baby Farm

Bay and the Gulf.

had a major overhaul and facelift that

• Work continued through MEERA, the

included a new conch nursery “raceway”

Marine Ecosystem Event Response

to demonstrate the aquaculture center’s

Assessment program that encourages

programs growing queen conch in

citizen observers to help deliver immediate

Summerland Key and Sarasota. Nursery

information about unusual environmental

trials examining the importance of

conditions to resource managers.

increased calcium in queen conch shell

• Collaborative research continued on

strength continued in collaboration with

Diadema antillarum, the long-spined sea

Harbor Branch Oceanographic Institution.

urchin, to determine how much this algae

The Conch Baby Farm is now a major

eater affects coral reef health.

attraction for Key West visitors.

• The lab provided field support for the

• The Florida Red Tide Monitoring

Center for Ecotoxicology’s coral health

Program assumed greater importance in

studies focusing on bacteria and fungi,

2004, sampling red tide events spanning

including hard and soft coral collection,

the Florida Keys.

1997 Mote and the

2003 Center renamed the Center for Coral Reef Research. Hard coral aquaculture research project begins.

2004 Conch Baby Farm opens in Key West as a public outreach facility to highlight Mote’s marine science research efforts in the Florida Keys and Sarasota.

microbiology sampling and controlled

PROGRAMS & MANAGERS CORAL REEF BIOLOGY, Erich Bartels, Staff Scientist. Evaluates the biology and health of coral reef ecosystems.

2004 Annual Report


MISSION: The Tropical Research Laboratory hosts the Center for Coral Research, which is dedicated to the restoration and preservation of coral reefs in collaboration with other research organizations in the Florida Keys. The Tropical Research Laboratory also maintains the Key West Conch Baby Farm and hosts research performed by other Mote centers and visiting scientists.


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MOTE MARINE LABORA LABORATORY

AQUACULTURE RESEARCH AND DEVELOPMENT AQUACUL Kevan L. Main, Ph.D., Director HISTORICAL HIGHLIGHTS

1957 Dr. Charles Breder, renowned fish biologist who first discovered that brine shrimp could be a larval food for marine fishes, joins Mote staff. 1986 Mote begins aquaculture research with common snook.

1996 Mote’s first aquaculture facilities are completed. 1998 Mote begins aquaculture research on Caspian Sea sturgeon.

Fish Farming Advances Mote Aquaculture Park expanded, focusing on testing and designing systems that use the best recirculating filtration systems to reduce the dependence and impact on coastal ecosystems and to design methods necessary for widespread commercial aquaculture production. • The second year of Florida pompano

the growth of restaurant-quality shrimp

studies was extremely successful,

designed to help fulfill an ever-increasing

demonstrating that this high-value marine

demand for this popular food. The goal is

Aquaculture Research and Development established. Construction begins on Mote Aquaculture Park 17 miles inland from the Gulf of Mexico.

fish can thrive in low-salinity conditions.

to farm Pacific white shrimp in low-salinity

Broodstock collected throughout the year

waters using recirculating techniques.

and housed at Mote Aquaculture Park

The program should also demonstrate the

were successfully matured, resulting in

viability of this species as a new “crop”

the production of large numbers of larvae

that can be grown inland, presenting a

and juveniles. The pompano successes

unique farming opportunity for Florida.

2002 First sturgeon

indicate that this species may be well-

• In addition to improving and expanding

production systems are completed at Mote Aquaculture Park and animals are moved from the Sarasota lab. Projects expand to include red snapper, Florida pompano and queen conch.

suited for inland farming in recirculating

marine aquaculture technologies, center

filtration systems. The continued

scientists are investigating the optimal

development of marine fish culture

biological and culture system needs of

techniques and technology will help

saltwater species and studying ways to

Florida’s aquaculture industry diversify

farm other marine species in low salinity or

the species that can be produced and

freshwater conditions. Using fresh water

marketed.

simplifies the requirements of recirculating

2003 Caspian Sea

• Large-scale growout systems for

systems and reduces production costs at

sturgeon are marketed, and hard coral aquaculture begins using new filtration and culture technologies.

sturgeon began operation, and fish were

inland sites.

spread into new commercial-scale tanks

• Growth of juvenile snook, red snapper,

with freshwater filtration designs. Data

queen conch and hard corals also

confirm that Siberian sturgeon grew faster

continued for stock restoration efforts.

2004 Marine shrimp

than initially projected and that Siberian

• The center’s recirculating system

demonstration project begins at Mote Aquaculture Park. Coral growth research focuses on field studies and environmentally controlled studies. Key West Conch Baby Farm is established to demonstrate queen conch growing efforts.

sturgeon can thrive in higher densities and

designs, along with research findings

higher temperatures than expected.

from aeration and filtration studies, were

• In 2004, the center began work on

presented at international conferences.

2001 Center for

2004 Annual Report

PROGRAMS & MANAGERS MARINE AQUACULTURE RESEARCH, Kevan L. Main, Ph.D. Develops the technology and husbandry techniques to farm highvalue species to advance Florida’s food fish aquaculture industry and works on techniques to help restore juvenile snook, red snapper, queen conch and coral populations in the world’s oceans. MARINE SHRIMP COMMERCIAL DEMONSTRATION, Ryan L. Gandy, Ph.D. Uses commercial-scale raceways and recirculating systems to produce shrimp in low salinity conditions. STURGEON COMMERCIAL DEMONSTRATION, James T. Michaels, Senior Scientist. Seeks to develop systems and techniques to produce caviar and sturgeon for luxury food markets. In so doing, the program will help relieve pressure on wild Caspian Sea sturgeon stocks and fill the current gap of supply vs. demand for food fish.


MISSION: Mote’s Center for Aquaculture Research and Development is finding innovative and cost-effective system and husbandry techniques to produce high-value marine and freshwater fish and shellfish. This research, using environmentally responsible practices, focuses on commercial-scale seafood production and growing fish for stock enhancement research.


8 18

MOTE MARINE LABORA LABORATORY

AQUARIUM

Daniel F. Bebak, VVice President HISTORICAL HIGHLIGHTS

1980 Mote Aquarium opens to the public.

1988 The shark tank exhibit is added.

1989 The JASON Project begins with real-time undersea exploration for students to view. The first Sarasota Airport aquarium opens.

1992 The original Mote Mobile Exhibit begins its travels. Rivers, Bays and Estuaries exhibit opens.

Expanding the Audience Mote Aquarium marked a milestone in 2004 with the opening of the “Mr. Mote Story” exhibit in recognition of the support and backing provided by Mr. William R. Mote. The exhibit links Mr. Mote’s history to the lab’s growth and expansion over the last five decades. • The new Immersion Cinema opened

identification using state-of-the-art remote-

in the Martin Selby Science Education

sensing equipment followed by divers

Center at Mote with expanded capacity

going down to identify, record, and assess

for an interactive movie-going experience.

possible archaeological sites. Those with

The new hi-tech cinema – the only one

the potential to yield significant information

of its kind in Florida – features a 40 foot

will be candidates for investigation through

1995 The airport gets

high-definition screen complete with Dolby

excavation, mapping and artifact recovery.

another Mote aquarium tank.

sound and interactive computer consoles

The program is building partnerships

that allow audience members to become

with other institutions and organizations

1996 The sea turtle

involved in the movies they watch. This

to conduct research in the Gulf of

new technology is designed to reach and

Mexico, the Florida Keys, T Tampa Bay and

teach young audiences in media they’re

Sarasota County. Future projects include

already familiar with – video games and

investigations of the prehistoric site at Little

with Hugh and Buffett opens.

big screen movies. The theater will also

Salt Spring with the University of Miami.

be used for interactive programs from the

• More people than ever were exposed to

1998 Mote Aquarium

Florida Keys National Marine Sanctuary,

Mote research through the aquarium and

is accredited by the American Association of Museums.

where Mote is working with the NOAA and

through the Mote Mobile Exhibit, each of

Mystic Aquarium to set up an interactive

which was seen by an estimated 400,000

reef camera.

people. A new off-site aquarium was also

provides more Mote Aquarium space.

• The Nautical Archaeology program was

unveiled at the Glenridge community.

established to add a cultural dimension

• Mote Aquarium hosted the Regional

1999 Unique arch

to Mote’s rich half-century of scientific

Aquatics Workshop, the only annual

aquarium installed in the Selby Public Library. The ray touch tank and the Sea Cinema open.

biological research and its interpretation

gathering of public aquarium facilities

to the public. Its first undertaking will be

managers to share their experiences, and

the Charlotte Harbor Shipwreck Survey,

supported the marine mammal and sea

2001 The Mysterious

a systematic survey of wrecks in the

turtle rehabilitation efforts during a record

Mollusks exhibit opens with Molly, one of only three preserved giant squid on display in the U.S. New Mote Mobile begins operation.

harbor. Operations will begin with target

year of strandings.

KEY EXHIBITS

2002 Contact Cove, a

A 135,000 gallon shark tank holds a variety of Florida fishes, including red drum, snook and goliath grouper. • Two touch tanks provide hands-on experience with living marine animals. • A giant squid display is one of only three in the country that shows this mysterious creature that has still never been seen alive. • Shark Attack Theater gives visitors a shark’s eye view of capturing and eating prey. • Research in progress takes place behind the glassed-in walls of working science labs. • Immersion Cinema combines gaming with movies to give kids of all ages a high-tech learning experience that teaches them about sea science and research. • Turtles and manatees offer an up-close glimpse of marine life.

1993 Wyland paints his famous whales and dolphins wall.

1994 The Ann and Alfred Goldstein Marine Mammal Center opens.

exhibit opens and rehabilitation hospital begins operation.

1997 Manatee exhibit

1998 & 1999 Expansion

volunteer-funded touch tank opens. Small-scale Immersion Theater is built and tested.

2003 Mote Aquarium accredited by the American Zoo & Aquarium Association. 2004 Annual Report


MISSION: The Mote Aquarium is more than just entertainment; it’s a learning experience about life in the sea. Its key mission is to link exhibits to the research being done by Mote scientists to help motivate interest and support in marine research. As the laboratory expands with new science programs and field stations, so too Mote Aquarium reflects this growth and evolution through new key exhibits. 30,000 people visited in 1980; now, an average of 30,000 people visit the aquarium each month.


8 20

MOTE MARINE LABORA LABORATORY

ANIMAL CARE PROGRAM

Charles A. Manire, D.V D.V.M., Manager HISTORICAL HIGHLIGHTS

1969 First year Mote staff worked with stranded marine mammals.

1973 A bottlenose dolphin becomes Mote’s first rehabilitation case.

1986 Mote’s first successful rescue and recovery of a stranded manatee calf.

1992 Mote’s first successful dolphin rescue and release; sea turtle rehabilitation efforts begin on a limited basis.

Breaking Animal Care Records The Animal Care Program cared for more animals in 2004 than in any previous year, breaking records in the number of animals successfully released back to the wild from both the Dolphin and Whale and the Sea Turtle Rehabilitation hospitals. • Staff and volunteers working in cetacean

had not previously been described in

rehabilitation had at least two dolphins

scientific literature.

in the hospital each day of 2004. Three

• There were 1,233 turtle hatchlings

dolphins were returned to the wild and

brought into the Hatchling Hospital during

Alfred Goldstein Marine Mammal Center opens and allows for more concentrated efforts with sea turtle and marine mammal rehabilitation.

three dolphins that had been rehabilitated

the summer of 2004, with more than

were declared unreleasable. Three

1,000 of them released. That makes a

additional dolphins were being held as the

90 percent release rate for the year. The

year ended for scheduled 2005 release. A

success can be partially credited to a

virus identified in one of these cases had

new treatment developed at Mote using

1996 Hugh and Buffett

never before been reported in cetaceans.

steroids to treat fire ant bites in turtles.

• The ability to treat multiple dolphins from

• In addition to being on public display,

more than one stranding will only increase

the resident sea turtles helped in a

in the coming years with the expansion

number of research projects involving the

of the Ann and Alfred Goldstein Marine

effects of environmental enrichment and a

Mammal Center, which includes a new

reproduction study. The turtles also helped

100,000 gallon rehabilitation tank.

rehabilitate stranded turtles, acting as

• Staff and volunteers in sea turtle

blood donors for transfusions on anemic

rehabilitation worked with 25 sea turtles

loggerheads brought in for treatment.

and were able to release 15 of them.

• Among the several research projects

Another three turtles will continue their

undertaken with the manatees was a study

rehabilitation into 2005. In addition, staff

of tactile acuity of their vibrasse (body

identified a new parasite – a lungworm

hairs). The level of tactile sensitivity that

tentatively being named Angiostoma

the manatees demonstrated is comparable

carettae – in loggerhead sea turtles that

to experienced Braille readers.

1994 The Ann and

arrive at Mote.

1997 The new Jane and David Allen Manatee Habitat opens as a new home for Hugh and Buffett and the Donna Wolf Steigerwaldt Rehabilitation Lagoon is completed. Two bottlenose dolphins are rehabilitated and released.

1998 Two rough-tooth dolphins and one sea turtle are rehabilitated and released.

1999 The Animal Care Program is officially established to combine the efforts to treat sick and injured animals and care for Mote Aquarium residents. Twelve sea turtles and one dolphin are treated and released.

2004 Annual Report

PROGRAMS & MANAGERS SEA TURTLE CARE, Corie Baird. Supervises the care of resident sea turtles. MANATEE CARE, Joseph Gaspard. Supervises the care and training of resident manatees. REHABILITATION, Petra Cunningham-Smith. Responsible for coordinating non-medical care of cetaceans and sea turtles in rehabilitation. VETERINARY LABORATORY, Lynne Byrd. Responsible for coordinating medical diagnostics and care of resident and rehabilitation animals.


MISSION: Mote’s Animal Care Program is a part of Mote Aquarium and cares for the resident manatees and sea turtles. The program includes a rehab facility for dolphins, whales and sea turtles and seeks to provide state-of-the-art critical and chronic care. The program seeks to learn everything it can to expand the knowledge of the basic biology, the veterinary care and the disease processes of these animals.


8 22

MOTE MARINE LABORA LABORATORY

EDUCATION DIVISION EDUCA

David H. Niebuhr, Ph.D., VVice President HISTORICALLY SPEAKING HISTORICALL From the beginning, Mote research and education programs have linked public fascination with the local marine environment to the work going on at the laboratory. From nearly day one, students came to the lab to learn about sea creatures, often bringing their parents along on the journey.

Encouraging Lifetime Learning The division interprets current marine research to local and global audiences using innovative long-distance and hands-on methods. By inspiring life-long learning about the sea, the division strives to motivate conservation and sustainability of our coastal and marine resources.

Residents and visitors alike have come to Mote with questions about what they found on the beach, saw in the water or smelled in the air.

Distance Learning

for the “Secret Life of Dolphins,” in a

The Center for Distance Learning brings

partnership with Chicago’s Brookfield

Mote science and educational programs

Zoo. The project was introduced to a pilot

to a wide variety of audiences through

group of teachers, and staff from Distance

interactive videoconferences and other

Learning and the Brookfield Zoo presented

Mote staff has always been receptive to these inquiries, and informal summer programs for children date from the lab’s earliest days.

distance learning technologies. The

the program at the Marine Mammal

SeaTrek Program targets K-12 students

Alliance Meeting in Orlando in July.

and their teachers and presented more

• Mote’s Immersion Cinema broadcast Dr.

than 130 videoconferences in 19 states.

Robert Ballard’s return to the Titanic Titanic in the

• The 2004 completion of the Keating

“Titanic Live! Expedition.” A special VIP

Organized on-site education programs began more than 20 years ago.

Marine Education Center created two

evening included a live videoconference

state-of-the-art videoconferencing

with Dr. Ballard. The evening also

studios. Funds provided through a U.S.

featured Dr. J. “Coz” Cozzi, Mote’s

Department of Education grant helped

nautical archaeologist, and Mote Trustee

complete the technological components

Mr. Robert Williams, who has visited the

of the presentation and studio spaces,

Titanic in a submersible. Titanic

helped improve electronic capabilities

• “Disappearing Wetlands,” the subject of

at the lab and provided internet and

the 2004 JASON Project, was broadcast

infrastructure support for Mote’s Tropical

from Louisiana and attended by a student

Research Center in the Florida Keys.

argonaut from Sarasota. Approximately

• The center began development of new

150 teachers participated in Mote’s

videoconferences on fisheries, coral reefs

JASON Expedition training classes.

and marine technologies. SeaTrek also

School and Public Programs

SeaTrek was a natural extension of the popular JASON Project that started in 1989 and features real-time undersea and terrestrial exploration. SeaTrek, started in 1996, has expanded from the original seven Sarasota schools that participated to 43 schools across Florida. Another 130 video conferences were presented in 2004 to students in 19 different states.

offers a new “Careers” program to educate middle and high school students about careers in marine research. • A videoconference, curriculum materials and software database were completed

These programs translate current Mote research into hands-on opportunities for students of all ages so they can explore environmental and conservation issues (cont’d.)

CENTERS & DIRECTORS CENTER FOR DISTANCE LEARNING, Elizabeth K. Metz. Reaches audiences across Florida and the nation to teach students about marine science topics. The SeaTrek program targets K-12 audiences and their teachers. Future audience development efforts include programming for pre-service teachers, marine resource managers and public visitors to other aquariums, museums and libraries. CENTER FOR SCHOOL AND PUBLIC PROGRAMS, Julie Childers. Teaches marine science programs for schools, families and professional and social organizations onsite at Mote. CENTER FOR VOLUNTEER AND INTERN RESOURCES, Andrea S. Davis. Affords direct training and hands-on educational experiences for participants while providing the lab with essential, expert assistance.

2004 Annual Report


MISSION: The Mote Education Division was reorganized in 2004 by the Board of Trustees as part of the Mote Marine Laboratory Strategic Plan. The Division now includes the Center for Distance Learning, the Center for School and Public Programs and the Center for Volunteer and Intern Resources.


8 24

MOTE MARINE LABORA LABORATORY

EDUCATION DIVISION, continued EDUCA HISTORICALLY SPEAKING HISTORICALL Throughout the years, volunteers and interns have played critical roles at Mote. Like many other programs, the volunteer and intern programs evolved informally.

and become informed decision makers

provides support for Mote research,

regarding the future of marine resources.

aquarium and education programs.

• A new partnership with New Gate

Additionally, interns and volunteers fulfill

Sarasota expanded programs for 10th

career or life interest aspirations through

and 12th graders. The students come to

active participation in Mote’s programs.

behind-the-scenes tours with each visit

• Restructuring the college/intern program

focusing on a different Mote research

as a center under a newly unified and

center. There are now three partner

rejuvenated Education Division was the

schools in the Sarasota area.

most significant development in 2004.

• The new “T “Technology of the Ocean” field

Since explaining the results of Mote’s

trip for high school students featured a

research and educating people about

look at the technology that makes science

its mission are among the highest goals

possible. There are have eight programs,

of the institution, the consolidation of all

As the lab expanded and the aquarium opened in 1980, the need for well-trained volunteers and interns intensified.

all in keeping with Sunshine State

the education programs into one division

instructional standards.

has enhanced this synergy of research,

• A new corps of volunteer instructors will

education and the aquarium working

allow multiple classes to run consecutively.

together. Volunteers and interns play a key

• Public Programs took off in 2004 with

role and major accomplishments include

Volunteers met that demand themselves by creating the original volunteer organization and training program.

the addition of Mote Family Programs that

enhancing the programs.

were successful throughout the fall and

• Volunteer training now emphasizes

into the spring. “SeaSnooze,” an overnight

communication skills and interpretative

program, was a popular choice for schools

techniques. In 2004, 1,500 volunteers

Back in the early days, five docents gave tours at Siesta Key as early as the 1960s. The earliest formal intern program saw three college interns assisting in research projects in 1986.

and scouts.

served in all areas from the gift shop and

• The seasonal Monday@Mote lecture

exhibit interpretation to research and

series, featuring Mote and guest scientists,

animal care. In addition, the Volunteer/

was well attended and included special

Intern Center also worked closely with the

matinee features so more people could

Center for School and Public Programs

participate. The series will be presented in

to recruit, train and schedule volunteers

the Immersion Cinema in 2005.

to serve as teaching assistants. A new

• Summer Program enrollment exceeded

volunteer brochure was designed and

expectations and was filled to capacity.

produced in 2004.

Camps were offered at Mote facilities in

• A record number of 126 college interns

Sarasota and Summerland Key and were

gained valuable “hands on” field and

attended by students and teachers from

laboratory experience. The addition of

the U.S. and overseas.

a part-time intern coordinator improved

During the years when Mote was on Siesta Key, a few college students conducted research projects at Mote and a few volunteers helped researchers and gave informal tours.

Today, more than 1,500 volunteers provide more than 200,000 volunteer hours to the lab each year and more than 120 student interns provide academic enthusiasm in support of Mote’s research, education and aquarium programs.

Volunteer and Intern Resources The Center for Volunteer and Intern Resources provides direct educational experiences for adult learners and

2004 Annual Report

the program by expanding recruitment, diversity and scholarship efforts and the overall evaluation and assessment of the program.


COMMUNICATIONS DIVISION COMMUNICA Susannah Costello,Vice VVice President

Speaking of Science While Mote researchers focus on sharing science through peer-reviewed publications, the Communications Division is dedicated to explaining Mote’s messages, studies and results to the general public by finding new distribution channels and unique partnerships. • In 2004, the Communications Division

of nearly 100. The campaign kicked off

reorganized to bring operations in line with

with press conferences with best-selling

a new strategic emphasis. By creating a

author Randy Wayne White and Cousteau

new Publications department, the division

grandson, Philippe Pierre Cousteau.

focused more on content development

Efforts included special events to generate

and made significant gains in the evolution

support among front-line staff in tax

of Mote Magazine. Page count increased

collectors offices across the state.

by 30 percent and the magazine was

• Electronic Media staff saw its first full-

redesigned. The improved magazine has

fledged documentary, Blue Holes, air

been well received, helping generate press

in Sarasota and Manatee counties and

coverage and opening the door for the

laid the groundwork for a new working

Development Division to submit several

partnership with WGCU Public Media

science grant applications.

based at Florida Gulf Coast University

• Using the production of Mote Magazine

to expand the reach of Mote science

as a tool, the division also launched Media

stories. Staff provided a full complement

Lab@Mote. The program, for college

of AV support and oversaw design

seniors and graduate students, is designed

and installation of new equipment in

as a practicum where journalism students

Immersion Cinema and the Keating

participate in the production of a science

Marine Education Center. Other audience

magazine and make contacts that will be

expansion efforts included a new Mote

important in their future science writing

Aquarium promo for Rocket TV TV, a web-

careers.

based video channel.

• Public Relations, now as a subgroup

• Design Services supported all of these

of publications, continued educating the

efforts by updating Mote’s visual branding

public about Mote science via the news

with the fresh new look that can be

media. The database of media members

seen in brochures, programs, posters

is now more than 400. Notables include

and banners throughout the lab and

National Geographic, the New Y York ork T Times imes,

aquarium. Designers have also improved

the Boston Globe, USA T Today oday,, CBS oday

color correction and other pre-press

Evening News, National Public Radio and

processes that are essential for providing

CNN, among others.

a professional look for all Mote materials.

• Marketing had an extremely successful

Work also started on editing and digitizing

year with the launch of the “Protect

a collection of 30,000 underwater

Our Reefs” license plate campaign. In

photos donated by Mr. Herman Gross for

its first year, the plate sales exceeded

eventual sale and licensing. Mr. Gross also

expectations by becoming the sixth best

supported initial efforts to catalogue the

selling plate in the state, out of a field

collection for production.


8 26

MOTE MARINE LABORA LABORATORY

DONORS The following companies provided gifts matching the contributions of their current and retired employees: Aetna Giving Campaign Allianz Life Insurance Company of North America Eli Lilly & Company Foundation Exxon Mobil Foundation Matching Gift Program General Reinsurance Corporation The Greater Kansas City Community Foundation IBM International Foundation Johnson & Johnson May Department Stores Company Foundation Microsoft Matching Gifts Program Pepsico Foundation The Prudential Foundation Matching Gifts Program Sam’s Club Foundation Verizon Foundation

2004 Annual Report

Meeting the Need In 2004, the following donor contributions of $500 or more through gifts, grants, memberships and in-kind goods and services helped support our ocean science research. $50,000 and up Anonymous Anonymous Judy Graham Michael and Franza Janes Janssen Pharmaceutica Edward and Elaine M. Keating Keating Family Foundation Mike McKee Family Myra Monfort Runyan Mote Scientific Foundation The New Amsterdam Charitable Foundation Roberta Leventhal Sudakoff Foundation, Inc. $25,000-$49,999 Anonymous Bank of America N.A. Client Foundation Juan Cobo Estate of Joanne Dowell Genova Products Mr. and Mrs. Alfred Goldstein Mr. and Mrs. Michael T. Martin Michael Saunders & Company Carol L. Miller Sarasota Ford Schering Plough Corporation YYellow Book USA $10,000-$24,999 ABC 7 - WWSB TV American Honda Motor Co., Inc. Bayer Healthcare LLC The Honorable and Mrs. Michael S. Bennett Susan C. Gilmore and Tim Clarke Combined Federal Campaign Conch Republic Seafood Company DEX Imaging, Inc. Disney Wildlife Conservation Fund Mr. and Mrs. Richard O. Donegan The Florida Aquarium Flotilla 82 USCG Auxiliary 10-2000 Mr. and Mrs. Charles Griggs Mr. and Mrs. Herman Gross The Hurlburt Family Foundation, Inc. The Mark and Carol Hyman Fund Inbar Designs, Inc. Item Development, Inc. Curtis W. Miles Charitable Trust Mr. and Mrs. Ron R. Morris Publix Super Markets Charities SinoFresh Healthcare, Inc. SonoSite Southern Elegance Cleaning Elizabeth Steele Wachovia Bank Robert and Jill Williams $5,000-$9,999 Anonymous Anonymous ALLTEL Communications ALL American Marine Holdings The Vernal W. & Florence H. Bates Foundation Bright House Networks Mr. and Mrs. Vernon G. Buchanan Karen Burns

David Byrne Mr. and Mrs. Mike Carter Charlie and Family Dr. Eugenie Clark Comcast Cablevision of West Florida, Inc. Jane C. Ebbs Thomas and Emily Franeta Galston Foundation Ketchum, Inc. Michael’s on East Gourmet Group Virginia A. Miller Kenneth & Myra Monfort Charitable Foundation, Inc. The New YYork Times Company Foundation, Inc. Northern Trust Bank of Florida Pat Carlton Construction, Inc. Dr. Alan Jon Smally and Dr. Sharon K. Pool Rabow Communication Arts Reliant Energy Foundation Mr. and Mrs. John Renaldi The ROS Foundation The Tillie Jennie & Harold Schwartz Foundation, Inc. Smith Barney Wallace M. Waddell Albert H. and Jane Wohlers Foundation Robert and Jeanne Zabelle Charitable Fund $2,500-$4,999 Abbott Laboratories RBC Centura Bank Joan Galvin and Jim Brown Susan Carr and Raegen Carr Mr. and Mrs. Howard C. Cobin Mr. and Mrs. G. James Creighton Custom Colors Powder Coatings Dolphin Aviation, Inc. John Henry Dorminy Foundation, Inc. Mr. and Mrs. Paul F. Eckel Mr. and Mrs. John Enander Historic TTours of America Icard, Merrill, Cullis, Timm, imm, Furen & Ginsburg, PP.A. Mr. and Mrs. Ronald A. Johnson Kirk-Pinkerton, PP.A. Jean Maguire Mr. and Mrs. Kevin McCormack Mr. and Mrs. G. Lowe Morrison Northwest Marine TTechnology Carole Pauly Paver Systems Reed Mariculture, Inc. Rocky Ross Sara Bay Marina Sarasota Herald-Tribune Peggy J. Sears Dr. and Mrs. H.A. Seider, Jr. Mr. and Mrs. Jay Stackig SunTrust Bank Tropicana Products, Inc. Underseas, Inc. James and Erika Waldron Williams, Parker, Harrison, Dietz & Getzen Willis A. Smith Construction $1,000-$2,499 1800Endoscope.com LLC Anonymous


David Altman Foundation Art & Frame of Sarasota Mr. and Mrs. E.W. Armstrong Badcock Home Furnishings Baker & Co., Inc. Mr. and Mrs. Charles R. Baumann City of Holmes Beach Mr. and Mrs. Steve Belack Mr. and Mrs. Glenn Bell Franklin G. Berlin Foundation, Inc. Mr. and Mrs. Robert A. Bernhard Jeff Brinkerhoff David Brittain Mr. and Mrs. Ken Bussard Marina Liem and Alex Cannon Mr. and Mrs. Ronald D. Ciaravella The Casey Key Foundation, Inc. Vera Cash Foundation, Inc. Coastal Chemical & Paper Supply Computerized Aircraft Maintenance Systems Deborah M. Cooley Charitable Trust Jane T. and Robert V. Corning Fund Cowles Charitable Trust Mr. and Mrs. Ken Cox Lt. Gen. and Mrs. Howard G. Crowell, Jr. (Ret.) Crystal Springs Petra A. Cunningham Mr. and Mrs. Michael L. Dow Dr. Sylvia Earle Richard E. Ehlis Mr. and Mrs. Douglas C. Elder Fergeson, Skipper Skipper, Shaw, Keyser Keyser, Baron & Tirabassi, PP.A. Dr. and Mrs. Arthur Finzel First State Bank Samuel Fowler Mr. and Mrs. Thomas J. Frazer Mr. and Mrs. Bruce Frerer Edith Gardner Mr. and Mrs. Joseph E. Gibbs Jane Graham-Hyslop Gulf Coast Community Foundation of Venice Coral Gwyn-Williams Jean P. Hendry Horizon Distribution, Inc. Mr. and Mrs. Bill Hunt Mr. and Mrs. Jeff S. Hunt Independent Charities of America Ervin Johnson, Jr. Richard P. Kahn Kennedy Electric Mr. and Mrs. John Lamoureux LaserSoft Imaging, Inc. Mr. and Mrs. Allan L. Levey Levin, TTannenbaum, Wolff, Band, Gates & Pugh, PP.A. Dr. and Mrs. Carlyle A. Luer Richard E. LLyke Charitable Trust Dr. and Mrs. Kumar Mahadevan Jean Cho and David Mankoff Marine Concepts Maj. Gen. and Mrs. Raymond E. Mason (Ret.) Joe McClash The Honorable and Mrs. Cornelius McGillicuddy Dr. and Mrs. Harvey Mendelow Ted C. Merritt The Honorable and Mrs. F. Daniel Miller Mr. and Mrs. James A. Mitchell Mosaic New Atmosphere Productions Mr. and Mrs. Richard Nimtz Mr. and Mrs. Fred Nobel Jeff B. Parker Mr. and Mrs. Lee Peakes Pfizer Foundation Volunteer Program Barbara L. Piper R. and M. Foundation Trust

RBC Centura Bank Regan Insurance RIGSA RIGSAVER Mr. and Mrs. Dana R. Robes Mr. and Mrs. Dean E. Rollings, Jr. Sam’s Club #4772 Sam’s Club #8201 Sarasota Bay Parrot Head Club, Inc. SeaSide Garden Retreat Mr. and Mrs. Harry R. Silverglide Mr. and Mrs. Peter Smith Elizabeth B. Stadler LaVerne Stellas Mr. and Mrs. Bayne Stevenson Sweetwater Kayaks Outdoor Center Vantage Consulting, Inc. Janet B. Walsh Gigi Wegner Mr. and Mrs. R. Elton White The Whitlock Group Wyman, Green and Blalock, Inc. Marcia John and Lincoln Zweig $500-$999 The Academy at Ocean Reef William F. Alexander, IV Anderson Industrial Equipment Anonymous June Apisdorf Linda Mecke Aronson The Atlantis Furniture Co. Mr. and Mrs. Donald G. Baker Mr. and Mrs. Edward C. Bavaria Chris and Dick Bayles Benchmark Blueprinting, Inc. Mr. and Mrs. William G. Bird Mr. and Mrs. Arthur D. Bissell, III Mr. and Mrs. Walter C. Bladstrom BoldWater, Inc. Anna Bracken Dr. and Mrs. Glenn L. Bredemeyer Dr. Lisa Brewer Mr. and Mrs. Abbott Buegeleisen Mr. and Mrs. Keith Calleja Caroline Cannarile Mike Carter Construction, Inc. Mr. and Mrs. Roger A. Chrisman Aleta D. Chrisman Hera Konstantinou and Dr. Joseph Cozzi Peggie Crawley Crystal Mesa Farms Lissy Dickens Mr. and Mrs. George W. Dietrich Mr. and Mrs. Bob Dods Mr. and Mrs. John C. Doremus, Jr. Mr. and Mrs. Steven Drell Drew Ericksen Essay Marine, Inc. Exel International, Inc. Fabulous Faux Jewelry Company Mr. and Mrs. Donald O. Featherman Fifth Third Bank Mr. and Mrs. Joseph F. Flannery, Jr. Mr. and Mrs. Edward H. Foss, Jr. Friends of Animals Foundation Garden Argosy, Inc. Janet E. Goettle Mr. and Mrs. Herbert Gold Grabill Painting & Drywall, Inc. Mr. and Mrs. Richard E. Goss Dorothy Greenlee Mr. and Mrs. John Hager Mr. and Mrs. John O. Hanna, Jr. Dr. and Mrs. Kenneth D. Henson Mr. and Mrs. William J. Hill Shiela Hingorani

Mr. and Mrs. Dieter Hoehn The Home Depot, Key West, Florida Laura Breeze and Van Huff Mr. and Mrs. Frank Hulswit David Janes Dr. and Mrs. Albert G. Joerger Mr. and Mrs. Robert M. Keil Keith’s Marine Mr. and Mrs. James R. Lambie Mr. and Mrs. Richard L. Leach Heather LeBlanc Lemon Bay High School Bernard Lewis Charitable Foundation The Honorable and Mrs. Gavin Litwiller Joyce W. Lockhart The Lombardo Foundation, Inc. Mr. and Mrs. J. Robert Long Alan L. Martin Mr. and Mrs. Thomas R. Marvel Mary’s On Main, Inc. Mazuri Foods Mr. and Mrs. Timothy I. McAllister Gladys McCallum Mr. and Mrs. Larry Merriman Mr. and Mrs. Robert E. Messick Mote Marine Laboratory Volunteers Dr. and Mrs. John H. Muehlstein Mr. and Mrs. Norman Napier The Naples Daily News William T. Noll Mr. and Mrs. James W. Norris Mr. and Mrs. Randy Norton Vera C. Plescia Hannah and John Puckhaber Mr. and Mrs. David G. Pyle Mr. and Mrs. Norman Reiter Jay R. Rhoads, Jr. Martin D. & Barbara H. Rich Family Charitable Foundation Mr. and Mrs. Fred M. Richman Randall Rockel Mr. and Mrs. Jack W. Rynerson Mr. and Mrs. Matthew T. Ryson Sanibel-Captiva Shell Club Sarasota Family YMCA Dr. and Mrs. Edward M. Schaeffer Mr. and Mrs. Michael M. Scharf Mr. and Mrs. William Schmidt Alice M. Shaw Mr. and Mrs. Doug Siemon Margaret A. Smith Sam Snead’s Tavern Mr. and Mrs. Robert P. Sondgerath Sonotronics Paul C. Steinwachs Mr. and Mrs. Charles Stevenson Nancy C. Stockton Martha Wood Stouky Mr. and Mrs. Gene Stover John M. Strickland Summit Drilling Co., Inc. George A. Symanski, Jr. Tappan Nurseries, Inc. Henry & Marilyn Taub Foundation Mr.. and Mrs. James TToomey Dr. Ralph Trine and Dr. Sheri Trine John G. & Anna Maria Troiano Foundation Tropex Plant Sales United States Surgical VALIC Andrew Vac Remax Excellence Jann Warfi arfield Jane P. Watson Woodward Foundation Rafael Haddock and Catherine L. Wright Wyland Galleries Wyman Plumbing, Inc.







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