Experimental Animal House Facilities DR FRANCIS XAVIER COLLEGE OF VETERINARY & ANIMAL SCIENCES MANNUTHY 680651
Mail me :francier@doctor.com
Location Isolated buildings located far away from human habitation. Not exposed to dust, smoke, noise, wild rodents, insects and birds. (Primates)
Topography -Level, drainage, sunlight and wind directions Availability of -Electricity, water etc. Accessibility -Air, road or rail Future expansion
Types of Animal House Facilities Conventional
facilities
Conventional facilities
Design and size Physical plant Scope of institutional research activities Animals to be housed Physical relationship to the institution Geographic location
Types of Animal House Facilities Isolator facilities – Specific Pathogen Free (SPF)
Types of Animal House Facilities Isolator facilities Specific Pathogen Free (SPF)
View of Clean Corridor
Physical relationship of AF to Labs •For good Animal Husbandry and human comfort and health protection we require • Separation of AF from offices, conference rooms and labs – by having separate building, wing, floor or room. • Animal rooms should occupy 50-60% of total constructed area and remaining for stores, washing, office and staff rooms, quarantine and machine rooms and corridors. • Macro and microenvironment to be carefully looked at.
Types of Animal House Facilities Barrier facilities
Individual Cages with Environment Control
Total space devoted to various functions One animal house 8617 sq ft
Mean of animal houses (10) 14980 sq ft
Animal rooms
50
46
Corridors
14
17
Service areas
16
14
Staff areas
08
09
Storage areas
12
14
Total area % devoted to
Single corridor design
CORRIDOR
STORE ROOM RABBIT ROOM
G. PIG ROOM RAT ROOM MICE ROOM
Functional areas 1. 2. 3. 4.
5. 6. 7. 8.
Quarantine – quarantine and isolation Breeding – separate rooms/wings/corridors for species Stock colonies – separate rooms/wings/corridors for species Experimental facilities – experimental rooms, diet preparation rooms, surgery, intensive care/postoperative care, necropsy, radiography, experimental manipulation/treatment, containment facility. Storage area – bedding, feed, water, racks, cages, bottles. Staff area – office, showers, sinks, lockers, changing area, toilets. Services area – sterilization equipment, cage repairs, wastes. Laboratory area – diagnostic labs for health and genetic monitoring, feed analysis, incineration area.
Physical facilities • Building materials • Corridors • Utilities – water lines, drain pipes, electrical fittings etc. • Doors • Windows • Floors • Drains • Walls and ceiling
AHF Design – double corridor
Cage or room size Species
NIH
UKHO
Mice
15 sq. in.
10 sq. in.
Rats
-
40 sq. in
G. pig
60 sq. in
73 sq. in.
Rabbits
3 sq. ft.
6 sq. ft.
Cats
2.5 – 3.0 sq. ft.
5.5 sq. ft.
Dog (med. Size)
12 sq. ft.
20 sq. ft.
Monkey (med. Size)
4.3 sq. ft. (2.5 ft. high)
7 sq. ft. (4 ft. high)
Animal House Facility
Minimum floor area recommended for laboratory animals (based on their weight/size and behavioral activity) Animal
Weight in grams
Floor area/Animal (cm2)
MICE
<10 upto 15 upto 25 >25
38.7 51.6 57.4 96.7
12
<100 Upto 200 Upto 300 Upto 400 Upto 500 > 500
109.6 148.8 187.0 258.0 387.0 >451.5
14
<60 Upto 80 Upto 100 >100
64.5 83.8 103.2 122.5
12
< 350 > 350
387.0 >651.4
18
RATS
HAMSTERS/GERBILS/ MASTOMYS/COTTON RATS
GUINEA PIGS
Cage height (cm2)
Insufficient cage size/overcrowding • Psycho-physiological stress – abnormal motor patterns – rocking, pacing, weaving and whirling. • Increased aggression • Disruption of sexual behavior • Cannibalism • Increased risk of infections – higher morbidity.
OVER CROWDING
Minimum floor area and height recommended for monkeys (rhesus and bonnet) based on their weight (size) and behavioral activity (for langurs, the recommended space is in the foot note below) Weight (in Kg)
Floor area Ft2
Cm2
Height (cm)
Up to 1
1.6
1440
50
Up to
3.0
2700
72
Up to 10-12
4.3
3870
72
Up to 12-15
6.0
5400
72
Up to 15-25
8.0
7200
90
Cage size
Recommended Space for Dogs Animals
Weight Kg
Floor area/ Height?in d Animal, ft2b
Dog
<15
8.0
-
Up to 30
12
-
>30e
>24.0
-
Personnel interaction in Animal House Research scientist
Animal House Manager
Veterinarian
Indian Veterinary Council Act (1984) Only persons registered with the Indian Veterinary Council/State Veterinary Councils can provide veterinary care to animals (IVC Act 1984 Section 30 a-d).