Biomolecular Crystallography

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NEW FROM GARLAND SCIENCE

www.garlandscience.com


The determination of protein structure is a key step to understanding molecular function in mechanisms of life, biotechnology, and the design of therapeutic drugs. Synthesizing over thirty years of advances into a comprehensive textbook, Biomolecular Crystallography describes the fundamentals, practices, and applications of protein crystallography. Deftly illustrated in full-color by the author, the text describes mathematical and physical concepts in accessible and accurate language. It distills key concepts for understanding the practice and analysis of protein crystal structures and contains examples of biologically-relevant molecules, complexes, and drug targ structures.

Biomolecular Crystallography will be a valuable resource for advanc undergraduate and graduate students and practitioners in structural bio crystallography, and structural bioinformatics.

Bernhard Rupp Garland Science November 2009 l 850 pages 448 full-color illustrations Hardback l 978-0-8153-4081-2 £60.00

Figure 13-14


Contents PART I: FROM SEQUENCE TO CRYSTALS 1. 2. 3. 4.

Introduction: Preparing Your Study Protein Structure Protein Crystallization Proteins for Crystallography

PART II: FUNDAMENTALS OF PROTEIN CRYSTALLOGRAPHY 5. 6. 7.

Crystal Geometry Diffraction Basics Statistics and Probability in Crystallography

PART III: FROM CRYSTALS TO DATA Figure 2-48

8.

Instrumentation and Data Collection

PART IV: DETERMINING YOUR STRUCTURE 9. 10. 11. 12.

get

Reconstruction of Electron Density and the Phase Problem Experimental Phasing Non-Crystallographic Symmetry and Molecular Replacement Model Building and Refinement

PART V: MAKING SENSE OF YOUR STRUCTURE

ced ology,

13.

Model Validation, Analysis, and Presentation

Key Features l

Written as a textbook for graduate-level courses and one-source manual

for students and practitioners in crystallography, structural biology, and structural bioinformatics.

l

Handsomely illustrated in full color by the author, over 440 modern graphs and

figures are used to explain complex subjects and draw from current examples in the literature.

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Presents a problems-oriented, hypothesis-driven approach.

l

Addresses the needs of structural biologists by describing the necessary

mathematical and physical concepts in an accessible, familiar language without neglecting accuracy.

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Contains numerous examples with a focus on drug target structures.

Thoroughly reviewed by experts in the field to ensure accuracy and currency.


Praise for Biomolecular Crystallography: “This is the book that molecular biologists and the crystallographic community have been waiting for.”

—Alexander McPherson, University of California at Irvine, USA

“Biomolecular Crystallography is first and foremost a comprehensive reference text and laboratory manual for the practicing structural biologist, from the basics of biomolecular structure to modern advanced and powerful techniques in biomolecular structure determination, and analysis and application of structural information. Figures and graphs computed from new and original data are used extensively to help clarify important concepts, and derivations of all relevant mathematical and statistical principles—many of which have never been brought together in a single volume—are presented. This fulfils a long-felt need!”

—Ian J. Tickle, Astex Therapeutics, Cambridge, UK

“This thorough treatment of modern macromolecular X-ray crystallography combines a comprehensive coverage of the fundamentals with methodological details that are often omitted in introductory texts. It will be of value to anyone that works in macromolecular X-ray crystallography, particularly to graduate students or postdoctoral fellows who are mastering the technique.”

—Mark Wilson, University of Nebraska, USA

“Given the extraordinary progress in biomolecular crystallography, the challenge of providing a comprehensive and authoritative overview, starting from first principles, is formidable. Dr. Rupp has, however, succeeded admirably.”

—Brian Matthews, University of Oregon, USA

About the Author Bernhard Rupp is the CEO and founder of q.e.d. life science discoveries in California. Dr. Rupp established the protein drug target crystallography group at the University of California Lawrence Livermore National Laboratory in 1993 and expanded it into high throughput crystallization and structural genomics with focus on drug target structures and structure guided drug discovery. He was also a founding member of TB Structural Genomics Consortium. Dr. Rupp is a consultant for several US biotech ventures, universities, and instrument manufacturers. He is a member of the U.S. National Academy of Sciences Committee for Crystallography and NAS Keck Futures Initiatives panel. Dr. Rupp lectures regularly at NSF sponsored workshops on Biomolecular Crystallography and at the EMBO PEPC workshops on protein expression.


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