Molecular Pathology MCQ 5 Using this technique, cores from hundreds of paraffin blocks are transferred into a recipient block used for high throughput immunohistochemical staining and analysis: A- Tissue microarrays B- DNA microarrays C- Western blot D- q-PCR E- CGH MCQ 6 To screen a particular tumour for genetic alterations (gains, losses), an appropriate test to do is: A. B. C. D. E.
Northern blot Western Blot Southern blot PCR Comparative genomic hybridization (CGH)
MCQ 7 Which of the chromosome on the right indicate a chromosomal translocation?
1. 2. 3. 4.
A B C D
EMQ 8 A. Quantitative PCR B. PCR C. Microarray D. Western blot E. Northern blot F. Southern blot G. FISH H. Comparative genomic hybridization I. Laser capture microdissection J. Proteomics 1. Which refers to the large-scale study of proteins, particularly their structures and functions 2. Allows quantitative measurement of the amount of DNA or RNA in a sample 3. Based on the hybridization of fluorescently labelled tumour DNA and normal DNA to normal human metaphase preparations. 4. It analyses copy number changes in the DNA content of a given subject's DNA/tumour cells. 5. A method for probing for the presence of a specific DNA sequence within a DNA sample