Identification of Phyto-Components Present in the Ethanolic Extract of Acacia Decurre

International Journal of Engineering, Management & Sciences (IJEMS) ISSN-2348 –3733, Volume-2, Issue-3, March 2015

Identification of Phyto-Components Present in the Ethanolic Extract of Acacia Decurrens (Green Wattle) Leaf Biomass Sakthi.G, Venkatachary Jayanthy  Abstract— The leaf of Acacia Decurrens (mimosaceae) was reported to be an ornamental plant. The aim of the current study was to characterize the bioactive constituents present in ethanolic leaf extracts of Acacia Decurrens which will be useful for its proper identification. FTIR (Fourier transform infrared spectroscopy Model-8400S spec.) is used to identify the important functional groups that are responsible for their activity. Infrared techniques provide non-destructive, fast, and low-cost analysis, and have shown promising results. Thus the study showed the presence of Alkenes and carboxylic acids.

II. RESULT AND DISCUSSION

Index Terms— Acacia Decurrens, Alkenes, carboxylic acids, infra red spectroscopy, Mimosaceae

I. INTRODUCTION Secondary plant metabolites (Phytochemical) previously with unknown pharmacological activities have been extensively investigated as a source of medicinal agents[3].

Phytochemicals like tocopherols, ascorbates carotenoids, and phenols present in the plants are strong antioxidants and have an important role in the health care system [2]. Phytochemicals from medicinal plants showing antimicrobial activities have the potential to fulfill their needs, because of their structural differences, and their mode of action are said to be very likely different. There is growing interest in correlating the phytochemical constituents of a medicinal plant with its pharmacological activity [1].

II. MATERIALS AND METHOD A. Plant sample extraction: Ten gram – powered sample is extracted with 30ml of 50% ethanol overnight and filtered in ash less filter paper. The extracted material is taken for FTIR analysis.

B. Spectral analysis of plant material: 1gm of dried plant sample was milled until fine powder was obtained and was filtered with sieves, KBr of spectroscopy grade were also filtered with sieves. Samples of 2gm were mixed uniformly with 100mg KBr (2% w/w) homogenized using stir vortex and was used for the analysis.

Fig. 1 Spectral study of Acacia Decurrens leaf biomass by FTIR

Manuscript received March 5 2015 Sakthi.G, Department of biochemistry, PSG college of Arts & Science, Coimbatore 641014, Tamilnadu, India Venkatachary jayanthy, Department of biochemistry, PSG college of Arts & Science, Coimbatore 641014, Tamilnadu, India

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Identification of Phyto-Components Present in the Ethanolic Extract of Acacia Deccurens (Green Wattle) Leaf Biomass

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Table 1 plant Functional group material peak in cm-1 1207 C- O carboxylic

2

1180

C- H alkenes

3

1099

C- O carboxylic

4

962

C- H alkenes

5

912

C- O carboxylic

s.no

FTIR carried out with Acacia Decurrens leaf biomass powder showed the presence of these functional groups in the sample carboxylic acid, alkenes, as seen in the figure 1 and table 9 below. FTIR analysis is used for the identification of phyto-components present in Acacia Decurrens leaf biomass.

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[3] Krisharaju, A, V., Rao T V N, and Sundararaju. 2005. Assessment of bioactivity of Indian medicinal plants using Brine shrimp (Altenaria salania) lethality assay. Int. J. Appl. Sci Engg. 2: 125-134.

[4] Gogoi SJ*, and Gogoi B Green Synthesis of Nano Particles from Leaves of Ethno Medicinal Plants -A New Approach Research Journal of Pharmaceutical, Biological and Chemical Sciences July-September 2013 Volume 4 Issue 3 Page No. 862 [5] Omotoso Abayomi E*, Ezealisiji Kenneth and Mkparu K. I. Chemometric profiling of methanolic leaf extract of Cnidoscolus aconitifolius (Euphorbiaceae) using UV-VIS, FTIR and GC-MS techniques Peak Journal of Medicinal Plant Research Vol.2 (1), pp 6-12, March, 2014.

[6] Feng Xu , Jianming Yu Tesfaye Tesso , Floyd Dowell , Donghai Wang Qualitative and quantitative analysis of lignocellulosic biomass usinginfrared techniques Applied Energy 104 (2013) 801–809

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