B2B Nutramedic&Cosmetics by InPharma

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Nutramedic &Cosmetics Summer 2022

Ingredients and raw materials Contract manufacturing Equipment & Packaging Services

Nutramedic &Cosmetics

Editor’s word

aam pleased to present the digital edition of the new magazine B2B Nutramedic & Cosmetics. The purpose of this journal is to connect manufacturers of active substances and other raw materials, packaging, machines for the production of food supplements, food, cosmetics, medical devices and medicines, and to provide readers with information about contract production services, dealerships and similar useful information. The journal will have a Croatian and English edition, intended for manufacturers of finished products in the mentioned categories in the region and beyond. We would like to thank everyone who helped create this sample. I hope that we will bring you interesting content in the coming editions, and that we will contribute to some new collaborations. We’re turning over a new business page, and I hope you’ll be turning the pages of this magazine with us.

Daria Šurić

M.Pharm.

B2B

Nutramedic &Cosmetics

Magazine for industry professionals in health, nutrition and cosmetics sector Ingredients and raw materials / Contract manufacturing / Equipment & Packaging / Services / Industry events www.nmc-magazine.com info@nmc-magazine.com Publisher: Darmell d.o.o. Cvjetna cesta 11, 10000 Zagreb, Croatia Mob: + 385 (0)91 6812444 darmell@protonmail.com www.dar-mell.com Supported by

Content Vitamin K The wellness vitamin

Viva Geneva Vitafoods event review

Luzixine™ A concentrate of benefits

Pomalive® Promising results in fight against dyslipidemia

Sinetrol® Adipose beiging benefits

Medical Plants Photo herbarium Sage

Getting Ahead of the Trend for Silicone-free Cosmetics

Acacia gum in gastrointestinal tract

Bioline Reliable partner for your projects Photoprotective effect of antioxidants on the skin Innovation & inspiration Fi & Hi Europe

22 27

www.inpharma.hr

Nutramedic &Cosmetics

Vitamin K The wellness vitamin

Vitamin K2 is an essential fat-soluble vitamin that directs calcium to the right places in the body. K2VITAL® K2 MK-7, the superior form of K2, activates the osteocalcin proteins that incorporate calcium to bones, and the matrix-Gla proteins (MGP) that bind calcium to prevent deposits in arteries and smooth muscle walls. K2VITAL® is offered in a range of formulations, including microencapsulated K2VITAL® DELTA for excellent K2 stability in mineral formulations.

hhealthy cardiovascular system affects personal well-being far beyond the prevention of serious cardiovascular disease. Clean, flexible arteries and blood vessels that can deliver nutrient-rich blood whenever and wherever it’s needed are the cornerstone of health and well-being. Whet her you’re participating in a fun run, playing with the kids or just carrying the groceries into the house, our bodies make certain demands in response to physical activity. Soft, flexible arteries that expand during exercise improve blood flow and increase oxygenation for sustained energy. This not only helps us perform the activity, but also affects how we feel while doing it.

AUTHOR:

Mathilde Pinon, Marketing Manager, Kappa Bioscience

If some degree of hardening is normal and affects the body’s response to physical activity, what does this say about the potential benefits of vitamin K2 MK-7? Can vitamin K2 have a decisive effect on the vitality and well-being of otherwise healthy individuals?

Heart & Blood - Engine and Fuel of our Life

Oxygen is a nutrient. It supplies the muscles and tissues with energy. During physical exertion whether climbing stairs or crossing the finish line more oxygen is needed. A healthy heart and a clean, flexible circulatory system can meet these intermittent demands placed by increased physical activity. They provide the body with oxygenated blood where and when it is needed. However, the mineral calcium can disrupt this system. While calcium determines the health of bones and teeth, it also has a drawback. When there is too much calcium in the body, it can build up in the form of calcium plaques in the arteries and blood vessels. However, according to studies, calcifications and vascular hardening develop gradually in a process measured over several decades. Initial signs of arterial calcification have been noted in otherwise healthy children. Studies have shown that nearly 30 percent of the U.S. population over the age of 452 suffers from calcium deposits on arterial walls. This condition typically worsens with aging3. What does it mean? That there is a realistic likelihood that individuals who are actually far from being in the risk group for CVD, or who have not yet reached the age of risk for

Nutramedic &Cosmetics CVD, may suffer some degree of arterial stiffness and loss of flexibility. Arteries and blood vessels may lose their stretching ability. This results in decreased blood flow when the body needs to supply more oxygen to the tissues. In this case, not only is there too little oxygen available, but the heart must work harder to pump blood through the blood vessels, which are stiff and reduced in diameter. In purely unscientific terms, we would describe or perceive this condition as “weak endurance.” It can prevent us from pursuing desired activities at the desired time and also affect how we feel about those activities. Calcium and age-related hardening of the arteries would therefore affect our own well-being in accomplishing or perceiving our everyday tasks and opportunities.

K2: The wellness vitamin

The role K2 plays in promoting wellness starts early and is not limited to benefits for our heart. K2 activates osteocalcin. This protein ensures that calcium reaches the bones. K2 ensures healthy bone growth in our childhood. This begins in the womb. Throughout our lives, K2 helps maintain strong bones. It keeps our bodies robust and resilient while protecting us from diseases such as osteoporosis. But what happens when we consume too much calcium due to our diet, dietary supplements or environmental factors (e.g. due to elevated calcium levels in drinking water)? How does Vitamin K2 restore the “calcium balance” to ensure that we can reap its benefits for good bone health while avoiding its negative effects on the cardiovascular system? Vitamin K2 MK-7 activates another protein that plays a crucial role in a healthy cardiovascular system. Vitamin K2 is indispensable for the activation of the calcium-binding protein matrix Gla protein (MGP)4. After activation of MGP by K2, MGP binds the excess calcium floating freely in the blood, preventing it from being deposited in the circulatory system5. Calcium-free arteries and vessels remain soft and flexible. They are ready to adapt to the demands of increased oxygen supply and increased blood flow due to physical activity.

The science of K2 and a healthy circulatory system

Various studies show a clear link between K2 and arteriosclerosis. High levels of unactivated MGP in the blood are associated with vitamin K deficiency and also with arterial calcification. In healthy adults, levels of unactivated MGP are related to age5, with people at increased risk for arterial calcification having greater amounts of unactivated MGP in their blood. Non-activated MGP inversely correlates with dietary low intake of vitamin K. Correlations between non-activated MGP and (lower) survival from HKL disease highlight the importance of MGP activation6. In a stud y of 577 elderly subjects, nonactivated MGP was associated with an increased risk of cardiovascular disease, and this risk was again attributed to low vitamin K levels7. Taken together, these studies show that high levels of unactivated MGP (with K2-dependent activation) are associated with vascular calcification.

However, further studies show that K2 supplemen ts cause a significant decrease in unactivated MGP in healthy individuals8-10. In addition, vitamin K2 provides significant enhancement of carboxylation (activation) of MGP in healthy adults at doses of 90µg and above. Finally, one study showed that the level of activated MGP increased significantly after taking vitamin K2 MK-7 at doses close to the recommended daily allowance (RDA 90µg and above)11.

Prevent calcification? Yes. But how can it be reversed?

K2VITAL® vitamin K2 MK-7 activates matrix Gla protein, which prevents calcification by binding excess calcium. But can it also reverse them? Can K2 restore flexibility to vessels and arteries, restoring them to their previous state of health and thus improving the body’s ability to carry blood and oxygen optimally? If so, surely vitamin K2 could actually “turn back time” by restoring the body’s ability to respond to physical activity. The role that vitamin K2 plays in prevention is supported by two large studies. Through the study conducted with more than 4,800 men and women in Rotterdam, it is shown that the risk of HKL disease and mortality risk are dramatically reduced with a diet rich in vitamin K2 . A study of more than 16,000 subjects has shown an inverse correlation between K2 intake and risk of coronary heart disease, with mortality risk reduced by 9% with each additional 10µg/day intake13. However, the Knapen study conducted in 2015 most clearly proves that calcification can be reversed. It has shown that a high intake of vitamin K2 MK-7 is related to the reduction of arterial calcification. It has further shown that existing arterial stiffness decreased (was reversed) within the test group over a 3-year period, while it increased in the placebo group14. The vitamin K2 used in the Knapen study is equivalent to K2VITAL®15. While many K2 studies have been conducted with average healthy, elderly or sick individuals, studies with healthier individuals have just begun. A study conducted in 2017 with highly trained athletes sho wed a 12% increase in performance, as measured by cardiac output, after taking the K2 MK-7 supplement for a period of just 8 weeks16. This increase is impressive considering that the basis of measurement is likely higher in trained athletes compared to average individuals, making it more difficult to determine the differences. Although the exact mechanisms of the results obtained with K2 need to be further explored, this study provides good evidence that vitamin K2 can impact the factors that ultimately influence our personal well-being.

An enormous market opportunity

K2 is one of only a few essential vitamins, and they are not discovered every day. Although K2 was discovered several decades ago, its importance to bone and heart health has only recently been understood. While K2 is found in some meats and cheeses, as well as certain fermented foods, most Western diets are considered to be low in K217. However, the greatest commercial advantage of K2 is its applicability ac ross many different consumer and market catego-

Nutramedic &Cosmetics Haemost, 2010. 104(4): p. 811-22.

ries. Both men and women need vitamin K2 at all ages, from childhood through the teenage and adult years and into old age. In children, K2 is important for the development of strong bones. Teenagers need it during their growth spurts. And for women, it’s (especially) important so they can build strong, maximum bone density to prevent future bone disease. Both sexes benefit from the cardiovascular benefits K2 provides. While this article focuses more on K2 in the area of wellness products, equally strong, if not stronger, arguments can be made for “healthy aging,” “women’s health,” “sports nutrition,” and other areas. From the general nutritional deficiency and the wide range of applications in diverse product and consumer categories, the market potential is enormous. Vitamin K2 provides additional health benefits when combined with four of the five key ingredients in dietary supplements - calcium, magnesium, omega-3 and multivitamin blends. Innovative dosage forms extend the reach of K2 beyond traditional tablets and capsules. Since K2 holds great promise for the “sports nutrition” and “active lifestyle” categories, it will most likely make a breakthrough here by being used as a fortifier in dairy products such as milk, yogurt, probiotic drinks, and sports drinks and bars. The development of light-strength provitamin K2 will only increase the use and acceptance of K2 in active lifestyle products.

Arguments in favor of K2 as a wellness vitamin

The benefits that K2 provides to the cardiovascular system through the activation of MGP are very promising for improving the overall health and well-being of healthy, active people. K2 prevents and reverses calcification in the circulatory system. This allows the body to meet the increased oxygen demands associated with physical activity. The increased endurance experienced during athletic or everyday physical activities can lead to an improved perception of overall well-being in healthy individuals. The market potential for K2 is enormous, whether it is used in the consumer segment, in the market category, or as an additional ingredient in formulations.

References: 1

Greenland, P., et al, ACCF/AHA 2007 clinical expert consensus document on coronary artery calcium scoring by computed tomography in global cardiovascular risk assessment and in evaluation of patients with chest pain: a report of the American College of Cardiology Foundation Clinical Expert Consensus Task Force (ACCF/ AHA Writing Committee to Update the 2000 Expert Consensus Document on Electron Beam Computed Tomography) developed in collaboration with the Society of Atherosclerosis Imaging and Prevention and the Society of Cardiovascular Computed Tomography. J Am Coll Cardiol, 2007. 49(3): p. 378-402.

Image, D.E., et al, Ethnic differences in coronary calcification: the Multi-Ethnic Study of Atherosclerosis (MESA). Circulation, 2005. 111(10): p. 1313-20.

IIribarren, C., et al, Calcification of the aortic arch: risk factors and association with coronary heart disease, stroke, and peripheral vascular disease. JAMA, 2000. 283(21): p. 2810-5.

Dalmeijer, G.W., et al, Matrix Gla protein species and risk of cardiovascular events in type 2 diabetic patients. Diabetes Care, 2013. 36(11): p. 3766-71.

Cranenburg, E.C., et al, Characterization and potential diagnostic value of circulating matrix Gla protein (MGP) species. Thromb

Ueland, T., et al, Undercarboxylated matrix Gla protein is associated with indices of heart failure and mortality in symptomatic aortic stenosis. J Intern Med, 2010. 268(5): p. 483-92.

van den Heuvel, E.G., et al, Circulating uncarboxylated matrix Gla protein, a marker of vitamin K status, as a risk factor of cardiovascular disease. Maturitas, 2014. 77(2): p. 137-41.

Cranenburg, E.C., L.J. Schurgers, and C. Vermeer, Vitamin K: the coagulation vitamin that became omnipotent. Thromb Haemost, 2007. 98(1): p. 120-5.

Schurgers, L.J., et al, Vitamin K-containing dietary supplements: comparison of synthetic vitamin K1 and natto-derived menaquinone-7. Blood, 2007. 109(8): p. 3279-83.

Theuwissen, E., et al, Low-dose menaquinone-7 supplementation improved extra-hepatic vitamin K status, but had no effect on thrombin generation in healthy subjects. Br J Nutr, 2012. 108(9): p. 1652-7.

Theuwissen, E., et al, Vitamin K status in healthy volunteers. Food Funct, 2014. 5(2): p. 229-34.

Geleijnse, J.M., et al, Dietary intake of menaquinone is associated with a reduced risk of coronary heart disease: the Rotterdam Study. J Nutr, 2004. 134(11): p. 3100-5.

Gast, G.C., et al, A high menaquinone intake reduces the incidence of coronary heart disease. Nutr Metab Cardiovasc Dis, 2009. 19(7): p. 504-10.

Knapen, M.H., et al, Menaquinone-7 supplementation improves arterial stiffness in healthy postmenopausal women. A double-blind randomised clinical trial. Thromb Haemost, 2015. 113(5): p. 1135-44.

Moller, M., et al, Bioavailability and Chemical/Functional Aspects of Synthetic MK-7 vs Fermentation-Derived MK-7 in Randomised Controlled Trials. Int J Vitam Nutr Res, 2016: p. 1-15.

McFarlin, B.K., A.L. Henning, and A.S. Venable, Oral Consumption of Vitamin K2 for 8 Weeks Associated With Increased Maximal Cardiac Output During Exercise. Altern Ther Health Med, 2017. 23(4): p. 26-32.

Vermeer, C., Vitamin K: the effect on health beyond coagulation an overview. Food Nutr Res, 2012. 56.

Kappa Bioscience AS is the pioneer in development and production of biologically active all-trans menaquinone-7 (vitamin K2 MK-7), marketed under the K2VITAL® brand name. Kappa’s innovation of MK-7 synthesis marks the commercial milestone to attain widespread consumer adoption. Effective synthesis production drives scalability, price reduction and a secure supply chain - with unmatched ingredient purity and documentation. Combined with other Kappa innovations such as the patented K2VITAL® DELTA microencapsulation process, which provides K2 stability in mineral formulations, K2VITAL® offers brands and manufacturers a path to broader market segments. www.kappabio.com

Vita Nova is the official representative of renowned foreign producers of the highest quality raw materials for the pharmaceutical and food industry, with a special focus on raw materials for food supplements. The offer includes minerals and mineral salts, vitamins and vitamin mixtures, plant extracts, emulsifiers and stabilizers, produced using the latest technologies throughout Europe. Vita Nova also offers its customers cooperation in the field of new product development and knowledge of the application of raw materials in production processes. www.vitanova.com.mk www.linkedin.com/company/2966991/

Nutramedic &Cosmetics

Viva Geneva

Informa Markets toasts successful Vitafoods Europe 2022 event Almost 700 exibitors and more than 15,000 attendees from over 130 countries took part to this year’s event. Vitafoods Europe will return in May 2023: the in-person event will be held in Geneva 9-11 May 2023.

lmost 700 exibitors and more than 15,000 attendees from over 130 countries took part to this year’s event. Vitafoods Europe will return in May 2023: the in-person event will be held in Geneva 9-11 May 2023. Informa Markets is pleased to announce the success of Vitafoods Europe 2022. Held both online (213 May) and in person at Palexpo, Geneva (10-12 May), 15,251 attendees from over 130 countries took part to Vitafoods Europe 2022 bringing back attendance to the 2019 level. The event aimed to inspire nutraceuticals professionals from around the world with the latest industry and scientific knowledge, as well as via event zones on ingredients, finished products, services and equipment. Almost 700 companies exhibited at the event, 100 of those for the first time. Also returning to the event were DSM Nutritional Products, Lonza Capsules & Health Ingredients, Chr. Hansen, NZMP (Fonterra), Gelita, KSM-66 Ashwagandha, Kemin, and Lallemand Health Solutions. Over 90 hours of content was presented via the online event platform and in-person event, with highlights including key market insights and analysis from Innova Market Insights, Mintel and FMCG Gurus, Diane Ray from The Natural Marketing Institute discussing consumers’ mental wellbeing, Karin Verzijden of AXON Lawyers on personalised nutrition, as well as focused Omega-3 and Probiotics content at the dedicated Themed Resource Centres. Julien Bonvallet, Brand Director at Informa Markets, said: “We’ve worked hard to make this the best edition of the show yet to help both visitors and exhibitors spring back to business. We continue to evolve Vitafoods Europe as one of our flagship events, to not only reflect key industry topics and trends, but also visitor feedback. For example, our visitors told us they wanted even more networking, so we added more space for this as well as brought back popular events like the Community Breakfast and 5k run. Our audience also told us that they wanted to hear from more diverse voices and gain an understanding of the big ideas and innovations changing the industry. Responding to this, we added a wider range of speakers and companies into the agenda this year – from Doctors, Scientists and R&D profes-

sionals to regulatory bodies, manufacturers and research organisations. “We also know that sustainability really matters to our audience. This is a key focus for us at Informa Markets too. As such, we continue to work to make Vitafoods Europe an even more sustainable event – from going paperless, reducing waste and increasing recycling, to using environmentally friendly show materials, and hosting focused content sessions and speakers. “Connecting back with industry peers and meeting new faces in Geneva has been superb – this is what makes Vitafoods a key event for nutraceuticals professionals. We’re delighted to be back at our usual May timings in Geneva too – there was a real buzz and energy around the show floor which was wonderful to see. As ever, thank you to all our visitors, exhibitors and speakers this year, as well as our on-site team and facilitators!” www.vitafoods.eu.com

Nutramedic &Cosmetics

Luzixine

a concentrate of benefits The French company LRD recently started to market innovative raw material Luzixine™ on the human food market. Luzixine™ is a natural cocktail of proteins, omega 3, vitamins and minerals that is guaranteed to be free of GMOs and has the characteristics of a “super-food”.

his new vegetable ingredient, made in France from a sustainable cultivation, is the only concentrated extract in the world produced from the lucerne leaf. Luzixine™ is a natural cocktail of proteins, omega 3, vitamins and minerals that is guaranteed to be free of GMOs and has the characteristics of a “super-food”. Its protein and iron-rich composition has already proven itself through the company’s long-standing commercial activity in developing countries and notably earned it recognition for its benefits in fighting malnutrition.

Luzixine™: the only food supplement of its kind in the world

Luzixine , the first commercial lucerne leaf extract designed for human consumption, produced and distributed by LRD, is the result of 10 million euros worth of investment and 15 year research and development. It is authorised to be marketed as an ingredient for food supplements is Europe in accordance with Directive no 2002/46 of 10th June 2002 transcribed into French law by Decree no 2006/352 of 20th March 2006, published in the OJ of 25th March 2006. ™

From an minimum and ecoresponsible process

The product is produced in France by means of a natural and responsible agro-industrial process (ensuring that no part of the plant is wasted) and the extraction process helps to concentrate all of the nutrients to enable Luzixine™ to take full advantage of the extraordinary composition of lucerne.

Much more than just a protein concentrate - A good plant-based protein source Luzixine™ is rich in proteins and in fact contains in average 54% easily absorbable proteins. It is also beneficial in terms of the amino acids it contains (Figure 1), which include the eight vital amino acids (AAs) that the body needs (46% of all AAs). In actual fact, it has a chemical score (CS) – the system used to assess the nutritional quality of the protein in relation to a benchmark chemical score determined by the WHO and equal to 100 – of 120, demonstrating its ability to provide essential amino acids, that is those that the metabolism cannot synthetise. The nutritional value of the proteins found in Luzixine™, characterised by their ability to be absorbed with carrying degrees of ease, is also assessed based on their digestibility, which varies from 7085% depending on the type. The PDCAAS score of Luzixine™ is between 0,84 and 1, depending on the variability of digestibility. By way of comparison, pea proteins have a score of 0.7 and wheat proteins 0.4. FIGURE 1

Luzixine™ aminogram

Nutramedic &Cosmetics - A natural source of polyunsaturated fatty acids, vitamins and minerals A daily intake of 10g (maximum dose) of Luzixine™ significantly contributes to the recommended daily intake not only of proteins but also of iron, calcium, manganese, omega 3, vitamins A, B9, K1, and E (Figure 2). FIGURE 2

Table of nutritional claims for a 10g dose

A super-food on the food supplement market

Luzixine™ is guaranteed to be GMO free, suitable for those on vegetarian and gluten-free diets and designed to be used directly as a food supplement or incorporated in the formulation thereof (Figure 3). It is particularly well suited to various applications in the food supplement market, as a super-food, or an alternative to animal proteins. DESIGNATION: Lucerne (Medicago sativa) protein or Alfalfa (Medicago sativa) protein COMMISSION DECISION of 13 Octobber 2009 authorising the placing on the market of a leaf extract from Lucerne (Medicago sativa) as novel food or novel food ingredient under Regulation (EC) No 258/97 of the European Parliament and of the Council (notified under document c(2009) 7641)

Helping to fight malnutrition in developing countries

LRD has been supporting the APEF (Association pour la Promotion des Extraits Foliares en nutrition) which helps fight malnutrition around the world) by

providing Luzixine™ for developing countries, and the partnership that the association has formed with Prof. Bertin of the “CHRU” Reims (France) university hospital has enabled LRD to obtain evidence of the nutritional benefits of Luzixine™. Four clinical studies performed between 2004 and 2009 confirmed its efficacy and its innocuousness in undernourished populations, where it is used as a food supplement. FIGURE 3

Luzixine™ at a glance

The observational data gathered on the ground in Peru, India and the Congo has highlighted an improvement in the state of health of undernourished populations as a result of consuming 10g Luzixine™, the main effect of which are as follows: • An improvement in cases of anaemia caused by an iron deficiency (Figure 4), • An acceleration in stature-ponderal growth in children and adolescents (Figure 5), • A reduction in respiratory infections and chronic diarrhoea Observational data impact of Luzixine™ consumption on anaemia of malnourished population FIGURE 4

Nutramedic &Cosmetics Observational data impact of Luzixine™ consumption on health status of malnourished population FIGURE 5

Pomalive

Promising results in fight against dyslipidemia Oral supplement containing Hydroxytyrosol and Punicalagin could help improve Dyslipidemia in an adult population without co-adjuvant treatment

Example of applications LRD offers ideas for innovative nutraceutical concepts based on Luzixine™, for major market targets. In each of the applications, Luzixine™ is associated with a complementary micronutrient for optimized formulas. LUZIXINE™ CYCLE+ A programme dedicated to women during menstruation. A 5 g stick format rich in iron, combined with magnesium and vitamin B6 to helo reduce fatique. LUZIXINE™ HEALTHY AGING A healthy dose of essential nutrients to preserve health capital. A formula enriched with zinc, in the form of 1 g tablets to be taken twice daily – two in the morning and two in the evening – to reach in the recommending daily dose of 4 g. LUZIXINE™ ENDURANCE To naturally optimise the training and recovery of athletes. A 10 g formula enriched with vitamin C, in the form of capsules, powder sticks or stand-up pouches to sprinkle on food or to add to shakes and snacks.

ccording to recently published data from a randomised, double-blind, placebo-controlled stu dy , supplementation with Euromed’s ingredient Pomalive®, a precise combination of standardized olive and pomegranate fruits extracts, could help to improve dyslipidemia, a condition resulting from an unbalanced distribution of different lipids (triglyce rides, total cholesterol, HDL cholesterol and LDL cholesterol). Researchers from the Nutrition Department, University Hospital La Paz (Madrid, Spain) found that oral intake of patent pending combined olives and pomegranate extracts (Pomalive®) for 8 weeks (n = 84) significantly reduced plasma triglycerides (p < 0.05), decreased LDL-C (p < 0.01) and increased HDL-C (p < 0.05) in middle-aged participants with dyslipidemia, without co-adjuvant treatment, and no adverse effects were observed. Currently, statins are the most effective class of drugs for improving lipid profiles but up to 10% of patients experience side-effects that reduce compliance, thus limiting the risk reduction potential. Therefore, safe and effective alternatives are of great clinical interest. According to the researchers, the described improvements in the plasma lipid profiles of dyslipidemic subjects could contribute significantly to the improvement in endothelial dysfunction, arterial prehypertension and hypertension, as well as circulating plasma levels of oxLDL, as reported in 2019 . Pomalive® is a patent pending precise combination of standardized polyphenols obtained from po megranate and olive fruits. Processed with an eco- friendly, water-only technology Pure-Hydro Pro cess®, it is free from contaminants, organic solvent residuals and toxins. The raw material of the selected fruits is grown locally in the Mediterranean region - close to the manufacturing plant - according to strict and transparent quality control protocols.

References:

Luzerne – Recherche et Développement (L.R.D.) Complexe Agricole du Mont Bernard – BP 149 51008 Châlons en Champagne Contact : Tel : +33 3 254 22 62 e-mail: lrd@luzerne-rd.fr www.luzixine.fr

Quirós-Fernández R et al. Nutrients. 2022 Apr 29;14(9):1879

Nutrients 2019, 11(3), 640; https://doi.org/10.3390/nu11030640

EUROMED S.A. is a leading producer of standardised botanical extracts and natural active substances for the pharmaceutical, nutraceutical, food and cosmetic industries. All extracts comply with worldwide GMP norms, international pharmacopoeias and regulations. www.euromedgroup.com

Nutramedic &Cosmetics

Sinetrol adipose beiging benefits ®

Weight management is and will remain a major concern worldwide. More than one billion people globally, including 1 in 5 women and 1 in 7 men will be living with obesity by 2030 (world obesity atlas 2022). Consumers are aware of the issues linked to obesity and 31% of global consumers say that they are currently on a diet to lose weight. (2021, FMCG Guru). The common belief that weight management is simply a matter of deprivation is obsolete. Today we know that the goal of improving and stabilizing body composition is primarily to improve the metabolic health and the quality of life of an individual. Dietary supplements are therefore a strategy to achieve these objectives healthily by contributing to weight management through multiple mechanisms. Fytexia a life science company expert in scientifically-supported micronutrients, delivers Sinetrol® a proprietary nutraceutical ingredient targeting the improvement of body composition. Fytexia delivers active ingredients supported with scientific proof to formulate finished products. The company’s mission is to reduce risk factors for non-communicable diseases and improve quality of life.

Sinetrol® – adipose beiging benefits

Sinetrol® is a natural combination of polyphenols extracted from citrus and guarana targeting body weight management. Sinetrol® reduces the excess of fat mass and improves long-term body composition. The ingredient is formulated from highly characterized bioactive polyphenols: naringin and hesperidin, expressed as flavanones > 40%, acting with a low dose of caffeine. Non-allergen, non-GMO, Halal, gluten-free and suitable for vegetarians, Sinetrol® is available in powder form at a daily dosage of 630 mg. Sold in more than 25 countries, Sinetrol® has been investigated in several clinical studies. The citrus

polyphenol extract has a granted approval by international authorities. The South Korean Ministry of Food & Drug Safety (MFDS)’s health claim approval recognizes the ability of Sinetrol to “help reduce bo dy fat” in the overweight population. It is also registered to Health Canada (NPN 80059820), with approved claims on its weight loss efficacy, and has several certifications.

Safe, characterized & effective

More than 10 years of research have been conducted to support the mechanism of action and clin-

Nutramedic &Cosmetics ical benefits of Sinetrol® on body composition, using gold-standard methodology during interventional studies with follow-up. Sinetrol® has been clinically investigated on more than 300 subjects including both Caucasian and Asian populations. The action of Sinetrol® relies on its patented mechanism of action on the physiological energetic pathway of lipolysis. It destores fats accumulated as triglycerides within white adipose cells and turns them into energy production within adipose tissue, which dissipates the released fats as heat. This effect of Sinetrol® avoids further restorage of fat within adipose tissue. Both in vitro and ex vivo studies demonstrated that the benefits of Sinetrol® are explained by two complementary mechanisms of action, driven by the ability of Sinetrol® to mimic adipose cell phenotype change from white to beige, i.e. from a storing category of adipose cells to an energy metabolism-driven adipose cell category. Subjects taking Sinetrol®’s supplementation increased their resting energy expenditure by 180kcal/ day. This consequently led to a significant drop of 5% of their excess body fat mass. Body composition is rebalanced with a significant improvement in the lean-to-fat-mass ratio. 65% of the total fat loss was from the trunk area: Index of Central Obesity decreased significantly. A follow-up after the end of the supplementation confirms that Sinetrol® supports long-lasting benefits. One month after the end of the supplementation: subjects continue to reduce body fat mass.

Sinetrol® Key outcomes • Proven mechanism of action: adipose beiging benefits • Increase in resting energy expenditure • Rebalanced body composition: significant reduction of body fat mass without lean mass loss • Significant results on body weight, waist and hip lines • Long-lasting benefits: subjects continue to experience adipose beiging benefits

Fytexia Group

Fytexia Group is an expert life science company in developing scientifically-supported active nutrients for healthcare products. Fytexia’s mission is to reduce risk factors for non-communicable diseases and mild infections by offering innovative nutritional supplements from our scientific research and inspired by recognized public health food practices. www.fytexia.com FYTEXIA SAS ZAE VIA EUROPA 3 rue d’Athènes 34350 Vendres – FRANCE Tel: +33 (0)4 67 21 90 98

Nutramedic &Cosmetics

medicinal plants photo herbarium

Sage

Sage has been used for medicinal purposes for thousands of years. The Latin name Salvia comes from the Latin “salvum” which means „to save“, so sage can be said to be a “savior” in many health disorders and ailments.

edicinal sage (Salvia officinalis L.) is a perennial semi-wild plant of the family Lamiaceae. The stems in the cross section are four-column or almost round, grow up to 70 cm in height. The root system is well developed. The leaves are simple, wrinkled, grayish green in color, covered with tiny hairs, oppositely arranged on the stems. the flowers are monoecious (bisexual), about 2-3 cm large, they are arranged 5-10 in apparent clusters inflorescences on top of the stems. It blooms from May to July, lasting about 20 days. It naturally grows on rocky hills in the coastal area and on the islands of the Mediterranean, but due to its exceptional healing value it is grown all over the world. Sage adores a lot of sun and does not like predominantly moist soil and should be watered modestly. It is resistant to drought and frost. Sage has a wide application from being an amazing cosmetic ingredient due to its anti microbial properties to being used as a flavoring agent in the food and beverage industry.

Use for medicinal purposes

Dried sage leaf and essential oil are used for medicinal purposes.

• •

• • •

Salviae folium or dried sage leaf. Ingredients: Mono and sesquiterpenes (essential oil) Diterpenes - carmazolic acid (in the acidic medium, carmazolic acid passes into carmazole. These two compounds are bitter substances of sage they are not distilled and do not pass into essential oil but are obtained only by extraction triterpenes: germaniol, ursolin and oleomolic acid. lamiaceae tannins: polyphenolic compounds rosemary acid type flavoni: luteolin, genkwanin

Taxonomy kingdom: Plantae

order: Lamiales

family: Lamiaceae genus: Salvia

species: Salvia officinalis common name

Common sage flowering time

V, VI, VII month

Salviae aetheroleum or sage essential oil

Yellowish to light green liquid with a characteristic smell and pungent taste. It is obtained by distillation by water vapor from Salviae officinalis. Ingredients: • 1.5-2.5% essential oil: • thujon • 1.8% cineol • camphor • borneol and bornyl-acetate • thymole and carvacrol (rarely)

Health benefits and usage

Antiseptic, fungicide, antiflogistic, abortive in higher dosage. Use only externally for rinsing and gargling. Sage extract is used in cosmetics: • against dandruff • as an antimicrobial • antioxidant • adstringent agent • for cleaning • as a natural deodorant • for skin conditioning • skin protection • skin soothing effect • as a tonic

References:

Ljekovita kadulja, https://www.plantea.com.hr/ljekovita-kadulja Prof.dr.sc.Zdenka Kalođera, Farmaceutsko-biokemijski fakultet, Farmakognozija II, Zagreb, 2006/2007.

Health benefits and usage

External - infuse, tincture, extract for rinsing and gargling, for inflammation of the gums, oral cavity and pharynx. Adstringens due to lamiaceae tannins. Used internally - inhibits the secretion of sweat. In larger doses over a long period of time, it should not be used due to the existence of a poisonous thujon. Pregnant women should not use it.

Nutramedic &Cosmetics

Getting Ahead of the Trend for Silicone-free Cosmetics Volatile silicones are on the way out of the cosmetics industry. Strict new regulations and increasingly eco-conscious consumers are driving a switch to silicone-free products. Why is this happening now? And what alternatives are there? There are two main driving forces for this trend: changing regulations and shifting consumer preferences.

hen it comes to regulations for silicones in cosmetics, we’re mostly talking about three commercial volatile cyclosiloxanes: D4, D5 and D6. They are attracting regulatory attention because they were (in case of D4) or continue to be commonly used ingredients in the beauty industry but do not have a positive ecological profile. This applies in particular to D4, which belongs to category 1 for chronic aquatic toxicity and has a mandatory classification as category 2, “toxic to reproduction”, indicating that it is harmful to humans. D5 and D6 are likewise persistent, not biodegradable, and ultimately harmful to the environment. Governments worldwide are restricting or sometimes prohibiting the use of these materials in cosmetics. While it is already forbidden to actively use D4 in cosmetic products according to the EU cosmetics regulation (EU/1223/2009), its presence is still possible as an unintended impurity arising from other raw materials. To cover the unintended presence of this type of substance, they are classified as a Substance of Very High Concern (SVHC) in the European Union, while the presence of D4 and D5 in a concentration equal to or greater than 0.1 percent has been prohibited for rinse-off cosmetics since February 2020. The European Chemicals Agency (ECHA) has now submitted a proposal to extend this restriction to D6, and to also include leave-on applications for all three cyclosiloxanes. When this legislation is approved, manufacturers will have five years to phase out these materials. Outside the EU, a similar regulatory focus is gathering momentum. As cyclomethicones have been under discussion for several years now, some companies have already excluded those structures from their formulations. Due to the recent regulatory developments, there is now fresh impetus to do so. By staying ahead of this regulatory trend, companies can tap into perception and high technical performance that are difficult to replicate. We are creating a portfolio of sustainable alternatives, i.e. without use of silicones – and not just the materials that

author: Michal Stepulak, PhD, Senior Technical Manager Personal Care, Head of Reginal Formulation Center, BASF

the regulators are focusing on. Our specialists are developing state-of-the-art solutions that make it possible to eliminate all silicones from cosmetics. Fully replacing silicones puts manufacturers in a strong position, because their formulations are ahead of whatever regulations might be introduced for silicones in cosmetics, anytime and anywhere in the world. Emollients are a great example. These ingredients play a key role in creating cosmetic products that have a pleasant skin feel and gratifying textures. Especially light textures are very popular among consumers. They also provide a protective layer on the skin, keep it moisturized, solubilize lipophilic active ingredients and crystalline UV filters, and help to disperse pigments. We reviewed the use of cyclosiloxanes in various recently launched skin care products and color cos-

Nutramedic &Cosmetics metics worldwide. Around 50% of facial foundations and about 20% of make-up removers contained at least one of these silicones – so we have now developed and tested sustainable alternatives for these two categories. For the facial foundation category, BASF experts formulated a tinted day care fluid that replaced a volatile silicone emollient with Cetiol® Ultimate (INCI: Undecane (and) Tridecane). This is an ultra-fast-spreading and 100% plant-based emollient that matches the silicone product’s results when tested in a W/O formulation for mechanical smear resistance, rain resistance, gloss kinetics, blemish coverage and color homogeneity. Using our sustainable alternative, we created a product with SPF 25 sun protection, a natural look and no color shift over time. It is also easy to distribute, and leaves skin feeling powdery and smooth. In the second category, our scientists created a biphasic make-up remover. It contains a mixture of three fully plant-based emollients that account for 39% of the overall formulation, replacing a volatile silicone ingredient that accounted for 39% of the previous formulation. The optimal mixture of sustainable emollients was designed using a digital modeling tool that applies artificial intelligence (AI). Tests showed that replacing the volatile silicone with our plant-based alternative mixture had no negative impact on the sensory properties of the make-up remover. It enabled the formulation of a silicone-free biphasic make up remover that provides excellent performance when removing various decorative cosmetics. It is also tear-free, non-sticky, suitable for cold processing, and contains content that is 98% of natural origin in line with ISO 16128. In fact, our formulation performed even better than the silicone-based product. Another ingredient that could potentially replace cyclomethicone in cosmetic products is Tridecane (Cetiol iSan). Like the previously described emollient, it is characterised by very high spreadability (2500 mm2/10 min) and similar volatility as cyclomethicone. Sensory tests carried out in a group of trained panellists confirmed convergent properties both, in case of parameters related to the stage of application of the cosmetic on the skin (spreadability, glide, stickiness, waxiness), and in case of parameters related to skin care effects (absorption, waxiness, powdery after absorption). An undoubted advantage of Tridecane is its smell, or rather the practical lack of smell, which means that perfuming formulations containing a high concentration of this emollient (e.g. colour cosmetics) is not a major challenge. This emollient can also be used as the main ingredient in sunscreen oils containing crystalline UV filters. Unlike cyclic silicones, it does not stimulate recrystallization of the filters over time. Tridecane has also been tested for irritating properties and comedogenicity. In the case of the first parameter, both in vitro tests using the EpiDerm skin model and an in vivo test conducted under dermatological supervision on 30 volunteers were used. The results of both tests confirmed the absence of irritant properties. A separate test, also carried out on volunteers, confirmed the absence of come-

dogenicity at concentrations of up to 10% in the finished product. This makes Tridecane a good emollient for problematic skin care products (sensitive skin, acne-prone skin). It can also be used in make-up removal products. In a test proving its effectiveness, Tridecane proved to be better than dodecane, which is often used in make-up removal products. We expect regulations covering the use of cyclosiloxanes to be introduced around the globe, and that these materials will eventually be banned worldwide. In Canada, for example, D4 is already in the spotlight due to its environmental toxicity. In light of the new European Chemicals Strategy for Sustainability (CSS), the latest approach within the European Green Deal aiming to ensure a toxic-free environment, we can expect similar restrictions to those placed on cyclosiloxanes to be issued or at least discussed for further substances in the same chemical group. That’s the future – but we can already achieve it today. Our sustainable alternatives offer companies in this industry a clear path to replace silicones in their formulations now – instead of waiting until they have no choice. However, we’re not just trying to stay one step ahead of regulators. We see the Clean Beauty trend as a massive opportunity for companies in this industry to future-proof their product range by responding to changing consumer expectations at an early stage. Cosmetics manufacturers that offer silicone-free, safe and biodegradable products now can position themselves as market leaders in the years to come. Our sustainable alternatives to volatile silicones open up attractive potential for our customers. And we are offering solutions that tap into this potential while delivering the performance and feeling that consumers expect.

www.basf.com www.carecreations.basf.com

distributed by: Cos Chem d.o.o., Podgaj 2, 10000 Zagreb, Croatia

www.coschem.hr contact mail: info@coschem.hr

Nutramedic &Cosmetics

Acacia gum

in gastrointestinal tract Fibregum™ is a non-digestible, high molecular weight polysaccharide, composed of a proteinaceous core and associated polysaccharidic fractions. It presents powerful health benefits associated with a high digestive comfort that will allow its consumption by a wider range of consumers. Introduction

With the rise of some diseases like diabetes, obesity, high cholesterol, high blood pressure, Irritable Bowel Syndrome (IBS) and Inflammatory Bowel Disease (IBD), consumers are increasingly attentive to their diet. Consumers are reaching for more natural products. One of the food industry’s answers is the “free from” products. Free from sugar, hydrogenated fat, salt, gluten, lactose, FODMAPs etc. Another answer is the product origin and its way of production (organic, GMO-free). Consumers are also looking for shorter and cleaner ingredient list (no synthetic ingredient, no additives…). The other orientation is to adapt their diet and to consume more beneficial ingredients, such as fibers. The American Diabetes Association and WHO agree that dietary fiber intake should be near 25-30 g/day for a healthy life style. A study showed that mean daily dietary fiber intake for 2007-2008 was 15.9 g/ day1. The advantage of a diet rich in fiber is not only related to the digestive process but also to a healthier intestinal tract. People who suffer from an unhealthy digestive tract experience uncomfortable conditions including heart burn, gas, constipation,

AUTHOR:

Zagorka Blaževska, M.Pharm, MBA, Vitanova

diarrhea, nausea, abdominal pain and flatulence. Digestive discomfort can be highly debilitating with a strong impact on overall well-being and quality of life. Fibers are considered to have some prevention effects on colorectal cancer and they also increase satiety sensation. Moreover fibers can enable additional positive health activities including colonic fermentation stimulation, reduction of cholesterolemia and reduction of postprandial glycaemia, as recently stated by the European Commission (Regulation (EU) n°2016/854).

The effect of the gut barrier on overall health

The gut barrier is one of the most important components of the immune system. Its main role is to absorb nutrients and to serve as one of our body’s most important barrier. It protects us from potential allergic reactions, as well as microbiological and chemical threats. Our intestine is composed of epithelial cells sitting on a matrix (intestinal wall). Our intestine is colonized by trillions of bacteria (microbiota). With the worldwide growth of bowel pathologies such as irritable bowel syndrome (IBS) and inflammatory bowel disease (IBD), the overall interest in gut health and more specifically the gut barrier has grown. Today, IBS and IBD are common digestive problems affecting both women and men. Nearly 20% of the world’s population is impacted by IBS1,2 and millions of people are suffering from IBD. To summarize one can consider that: The incidence of impaired and increased intestinal wall permeability, also known as leaky gut syndrome (LGS), is now closely studied because of its potential involvement in many health issues and diseases.

Gut barrier functions

Our body is colonized by commensal microorganisms that interact with organs to maintain our natural barriers against external factors. Our body disposes of two main natural barriers: • Our first line of defense is our skin (our biggest

Nutramedic &Cosmetics organ). Here reside several microorganism communities that contribute to our immune protection. • The second barrier is our intestine, which is composed of a mono-layer of epithelial cells sitting on a specialized extra cellular matrix (intestinal wall). Our intestine is colonized by trillions of bacteria (microbiota). This barrier is involved in both innate and adaptive immune responses and is able to control the passage of nutrients, water, ions and macro molecules. The intestinal lining is constantly exposed to a vast array of microbes, food antigens and toxins. Gut microbiota contributes to our immune system by allowing us to tolerate a large amount of antigens. Gut microbiota + Intestinal wall = Gut barrier Day after day, one of the most important roles of this barrier is to discriminate between pathogenic and non-pathogenic compounds. Intestinal inflammation compromises the gut barrier and alters gut impermeability. Alteration of gut permeability, or impermeability, is known as leaky gut syndrome (LGS, figure 2). In this case, not all the “unauthorized” invasive molecules are blocked by the intestinal barrier, and some penetrate our organism.

What are the health implications of leaky gut syndrome (LGS)?

Much of the work in the area dealing with microbial interactions with host physiological processes are of great interest. Several studies have shown gut microbiota support energy metabolism and immune functions in the host. Furthermore, the concept of a “super-organism” has emerged. This concept reflects the physiological importance of mutually advantageous host-microbe interactions. When gut microbiota equilibrium is altered, several gastrointestinal and extra intestinal diseases can occur. This may be explained because gut microbiota interacts with the immune system. LGS is associated with a wide range of general symptoms, such as fatigue, fevers of unknown origin, abdominal pain, bloating and diarrhea, memory problems, concentration difficulties, and poor tolerance to exercise. Some recent studies and experi-

ments have considered the involvement of LGS in chronic heart failure4, irritable bowel syndrome5, inflammatory bowel diseases6, alcoholic dependence7, diabetes mellitus8, depression9, “sickness behavior”, chronic fatigue syndrome (CFS)10, and other autoimmune diseases.

Fibergum™ – acacia gum

Fibregum™ is an all-natural source of soluble dietary fiber (able to dissolve in water). Fibregum™ is the only Non-GMO project verified acacia fiber. It is dried exudation obtained from the stems and branches of carefully selected acacia trees. Fibregum™ offers a guaranteed minimum of 90% soluble fiber on a dry weight basis, using the traditional testing method AOAC 985.29. It is a non-digestible, high molecular weight polysaccharide. It is composed of a proteinaceous core and associated polysaccharidicfractions. It is made up of neutral sugars and uronic acids (95 % of the dry matter), protein (1% to 2%, depending on the species), polyphenols (catechins, epicathechins, etc.) and minerals (magnesium, potassium, calcium, sodium). It has a very complex structure with an average molecular weight varying from 300 to 800 kDa.

Demonstration of the comfortable digestibility of Fibregum™

In a study (Cherbut et al2) it was reported that Fibregum™ did not induce adverse gastrointestinal effects even when consumed at high doses, and demonstrated a bifidogenic activity. The study also showed a high digestive tolerance of Fibregum™. In this study, Fibregum™ did not induce flatulence below the dose of 30 g/day and daily doses higher than 50 g/day did not provoke any abdominal cramps or diarrhea. The study also demonstrated that Fibregum™ stimulates the growth of lactic acid bacteria starting from 10 g/day. Progressive fermentation - To further understand the intestinal tolerance of Fibregum™, Nexira conducted an in vitro experiment3. Fibregum™ has been demonstrated to be gradually fermented in the distal colon (ie transverse and descending colon). It has been confirmed that Fibregum™ is only fermented by lactic bacteria in the large bowel. The study also showed that Fibregum™ was fermented in the transverse colon and that a residual part was still available for fermentation in the descending colon. It was also observed that an adaptation of the metabolism of bacteria increased their capability in fermenting Fibregum™ during the 3 weeks of the experiment.

Methods – experiments made for the gut barrier

Reinforcement of the gut barrier by Fibergum – 1. Experiment In 2015, Nexira conducted new and ambitious experiments to demonstrate innovative physiological properties of Fibregum™ on gut permeability.

Effect of Fibregum™ on gut microbiota Fibregum™ has been shown to increase the intestinal population of Bifidobacteria and Bacteroidetes, known to be commensal healthy bacteria, and more

Nutramedic &Cosmetics specifically the anti-inflammatory bacterium, Faecalibacterium prausnitzii11.

Effect of Fibregum™ on short chain fatty acid (SCFA) production Fibregum™ has been shown to induce an increase in total SCFA production and mainly exerted a butyrogenic effect in the distal colon. These results support the conclusion that the distal colon is the main area of bacterial fermentation of acacia fiber. Effect of Fibregum™ on inflammation Under lipopolysaccharides (LPS) stimulation, experiments indicated that Fibregum™ reduces the pro-inflammatory cytokines and, in a complementary way, increases the anti-inflammatory cytokines. These results demonstrate that the anti-inflammatory effect of Fibregum™ is based on two combined actions: inhibition of pro-inflammatory cytokines and stimulation of anti-inflammatory cytokines12. Intestinal gut permeability modulation Samples collected during this experiment were used in a cell line model to assess potential gut wall modulation, specifically impermeability. Fibregum™ demonstrated a protective effect on barrier integrity as shown by an enhanced cell impermeability₁₃,₁₄ Reinforcement of the gut barrier by Fibergum, new experiments In 2016, in order to complete its scientific knowledge on the gut impermeability, Nexira worked with the INSERM (Institut National de la Santé Et de la Recherche Médicale) to carry out new experiments. This recognized scientific organization is the French national institute specialized in health and medical research. These new experiments were dedicated to research the mechanisms of the gut impermeability restoration, at a cell level. This second step was scheduled in Nexira’s R&D program. Samples were made during the first experiment so results can be linked. Donors of this experiment were suffering of IBS and IBD.

Tight junctions Tight junctions are different types of proteins (Claudin-1, Zonula Occludens (ZO-1) and Occludin). They consolidate the paracellular barrier that con trols the flow of molecules in the intercellular space between the epithelium cells. They are the closely associated areas of two cells whose membranes join together forming a virtually

impermeable barrier to fluid. Tight junctions are widely studied for their implication in the gut permeability in the IBD15 and for the involvement of the gut microbiota in their permeability16. How to assess gut permeability Trans Epithelial Electrical Resistance (TEER) measures electrical resistance through a membrane to assess the global quantitative impermeability. The higher the electrical resistance is, the more the cell line is impermeable.

Effects of Fibergum on gut

Effects of Fibergum on gut permeability on IBS subjects During the previous experiment, Fibregum™ fermentation products were sampled on the descending part of the artificial colon. By using two complementary technologies, TEER and FITC-dextran, it has been confirmed that these samples can significantly reduce the cell line permeability.

This activity was measured and confirmed by the two technologies. During this experiment, the activity at the tight junction level has been assessed with the protein expression. The Claudin-1 and ZO-1 revealed a trend of increase. The Occludin increased significantly. Furthermore, we measured an increase of the ARNm coding for the Occludin. In conclusion, Fibregum™ has a positive effect by increasing the number of Occludin tight junctions and thus by improving the gut impermeability. Effect of Fibergum on the inflammatory response on IBS subjects The anti-inflammatory effect of the Fibregum™ fermentation has been tested both on the apical and basolateral sites of the cells. The cytokines concentration at the basolateral level were too low to be measured. At the apical level, IL-6 and TNFα, two pro-inflammatory cytokines, showed similar profiles. The IL-6 concentration has been reduced in the three part of the colon. The TNFα concentration has only decreased in the transverse and descending parts. In conclusion, the Fibregum™ fermentation, occurring in the transverse and descending colon, has a positive effect by reducing the pro-inflammatory cytokines. Effects of Fibergum on the gut permeability on IBD subjects By using two complementary technologies, TEER and FITC-dextran, it has been confirmed that the fer mentation of Fibregum™ is able to significantly re-

Nutramedic &Cosmetics duce the cell line permeability. This activity, measured and confirmed by the two technologies, has sta tistically significant results (p<0.05). However, for the IBD donors, the improvement of the gut permeabi lity was only observed in the distal part of the colon.

References:

Effects of Fibergum on the inflammatory response on IBD subjects The anti-inflammatory effects of the Fibregum™ fermentation has been tested both on the apical and basolateral sites of the cells. The IL-8, IL1β and TNFα, all pro-inflammatory cytokines, showed a significant reduction (see table) in the distal part of the colon, at the apical level of the cells. Only a trend was observed for the IL-6.The anti-inflammatory cytokine IL-10 was increased in the distal colon. Fibregum™ has a positive effect on the inflammation by reducing the pro-inflammatory cytokines and by increasing the anti-inflammatory cytokines. Conclusion on the new experiments This new experiment confirmed that the Fibregum™ fermentation in the distal part of the colon exerts beneficial effects on the improvement of the gut impermeability and inflammation. This experiment provided new information on the mechanism invol ved at the cell level. It indicates that the Fibregum™ fermentation may exert its activity by reinforcing the tight junctions and by providing its anti-inflammatory effect at the apical level of the cells.

General conclusion

Nexira has conducted experiments on the acacia gum fermentation in the colon. The Fibregum™ fermentation has positive activities by reinforcing the tight junctions and by providing anti-inflammatory effect at the apical level of the cells. These results bring new hypothesis. The acacia gum fermentation provides well-known products such as butyrate, acetate etc. This fermentation should also provide other products whose exact nature is still unknown. These other products are supposed to act directly at the cells level and on the tight junctions. It has also been demonstrated a positive effect on the microbiota and the reinforcement of the gut impermeability. Hence, acacia gum is a very beneficial fiber with a high digestive tolerance. To summarize, Fibregum™ presents powerful health benefits associated with a high digestive comfort that will allow its consumption by a wider range of consumers.

J Acad Nutr Diet. 2012 May;112(5):642-8. doi: 10.1016/j.jand.2012.01. 019. Epub 2012 Apr 25. Trends in dietary fiber intake in the United States, 1999-2008. King DE1, Mainous AG 3rd, Lambourne CA

Cherbut C, Michel C, Raison V, Kravtchenko T & Severine M (2003) Accacia gum is a bifidogenic dietary fiber with high digestive tolerance in healthy humans. Microbial Ecol Health Dis 15, 43–50.

Terpend K, Possemiers S, Daguet D and Marzorati M (2013) Arabinogalactan and fructo-oligosaccharides have a different fermentation profile in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®). Environ Microbiol Rep Aug;5(4):595-603.

Krack A, Sharma R, Figulla HR, Anker SD., The importance of the gastrointestinal system in the pathogenesis of heart failure., Eur Heart J. 2005 Nov;26(22):2368-74.

Zhen Zhang Y. and Li Y.Y., Inflammatory bowel disease: Pathogenesis. World J Gastroenterol. 2014 January 7; 20(1):91-99.

Nahidi L, Day AS, Lemberg DA, Leach ST., Differential effects of nutritional and non-nutritional therapies on intestinal barrier function in an in vitro model., J Gastroenterol. 2012 Feb; 47(2):107-17.

Leclercq S, Cani PD, Neyrinck AM, Stärkel P, Jamar F, Mikolajczak M, Delzenne NM, de Timary P., Role of intestinal permeability and inflammation in the biological and behavioural control of alcoholdependent subjects., Brain Behav Immun. 2012 Aug; 26(6):911-8.

Vaarala O, Atkinson MA, Neu J., The “perfect storm” for type 1 diabetes: The complex interplay between intestinal microbiota, gut permeability, and mucosal immunity. Diabetes. 2008 Oct; 57(10):255562.

Maes M, Kubera M, Leunis JC., The gut-brain barrier in major depression: Intestinal mucosal dysfunction with an increased translocation of LPS from gram negative enterobacteria (leaky gut) plays a role in the inflammatory pathophysiology of depression. Neuro Endocrinol Lett. 2008 Feb; 29(1):117-24.

Maes M, Coucke F, Leunis JC., Normalization of the increased translocation of endotoxin from gram negative enterobacteria (leaky gut) is accompanied by a remission of chronic fatigue syndrome., Neuro Endocrinol Lett. 2007 Dec; 28(6):739-44.

Sokol H, Pigneur B, Watterlot L, Lakhdari O, Bermúdez-Humarán LG, Gratadoux JJ, Blugeon S, Bridonneau C, Furet JP, Corthier G, Grangette C, Vasquez N, Pochart P, Trugnan G, Thomas G, Blottière HM, Doré J, Marteau P, Seksik P, Langella P., Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients., Proc Natl Acad Sci U S A. 2008 Oct 28; 105(43):16731-6.

David Daguet, Iris Pinheiro, An Verhelst, Sam Possemiers, Massimo Marzorati, Acacia gum improves the gut barrier functionality in vitro. Agro FOOD Industry Hi Tech - vol 26(4) - July/August 2015: 2933.

Daguet D., Pinheiro I., Verhelst A., Possemiers S., and Marzorati M., Arabinogalactan and fructooligosaccharides improve the gut barrier function in distinct areas of the colon in the Simulator of the Human Intestinal Microbial Ecosystem. J.Fuct. Foods, 2016;20:369-79.

Patent pending N°WO 2015/162447

Lee SH., Intestinal permeability regulation by tight junction: implication on inflammatory bowel diseases, Intest Res. 2015 Jan;13(1):11-8.

Ulluwishewa D, Anderson RC, McNabb WC, Moughan PJ, Wells JM, Roy NC., Regulation of tight junction permeability by intestinal bacteria and dietary components., J Nutr. 2011 May;141(5):769-76.

Nutramedic &Cosmetics

Bioline

Reliable partner for your projects Bioline is an independent company specializing in providing a variety of pharmaceutical services.

hrough its representative office, it provides the opportunity to cooperate with a number of selected internationally renowned pharmaceutical companies and manufacturers of medicines, medicinal products, food supplements, cosmetics and food. As part of our offer, we offer a variety of potential projects and a variety of pharmaceutical forms tablets, capsules, sticks, orosolubile granules in stick, soft gelatin capsules, water-soluble powder sachets. With headquarters in Ljubljana, Slovenia and a partner company in Croatia, we cover numerous markets throughout the region: Slovenia, Croatia, Bosnia and Herzegovina, Serbia, Kosovo, Macedonia and Montenegro. We have been present on the market for 23 years, with always constant quality of service, which has been recognized by many satisfied customers. The companies that are service providers have been selected with care and emphasis on quality, innovation, flexibility and speed of delivery of the desired products. These are refurbished companies from Portugal, Italy, Poland, Spain. Bioline participates in all projects, giving them additional value in the form of proposals for new product concepts, thanks to many years of experience in the international market. We regularly visit all major trade fairs in the industry (CphI, Vitafoods, Health Ingredients, Food ingredients) to keep up with the news and maintain contacts with customers and service providers. So far, we have participated in the successful implementation of more than 50 projects. The services we offer to our partners provide the opportunity to always find the most suitable solution. In the category of food supplements we can offer: • Granule-based products: effervescent tablets, wa ter-soluble granules, water-free dissolving granules • chewable tablets, fast-dissolving tablets, sublingual tablets, effervescent tablets, coated tablets, tablets • Capsules • Powder • Spray • Drops

• Twist off soft gelatin capsules • Soft Gelatin Capsules • Packaging: sachets, blisters, glass bottles for tablets, tubes, secondary packaging in boxes. • • • •

Bioline Prečna ulica 6, Ljubljana, Slovenia MOBILE PHONE:

+ 386 41 663 347 E-MAIL:

plus.bioline@siol.net

In the category of medicines: Capsules Granules: effervescent granules, granules Tablets: Film-coated tablets, tablets Powder

In the category of cosmetics, biocides, medicinal products: • Liquids, gels, creams, ointments, pastes, wipes • Shower gel, cream bath, shower scrub, liquid soap, intimate care soap • Creams, milks and oils for the body • Creams, serums, lotions, tonics, micellar face water • Toothpastes and tooth gels • Mouthwash • Wipes • Sun protection • Eau de Parfum, scented waters, aftershave, deo roll-on, deo stick, deodorant spray • Natural and BIO sanitizing and disinfectant products • • • •

Medicinal products for oral use: Soft Gelatin Capsules Spray Syrups Drops

Our advantages includes: ✓✓Product placement rate - discover our range of ready-to-market products; ✓✓Innovation laboratory - “out of the box” thinking; ✓✓Superior taste masking technology, excellent texture, color, quality and stability (stability studies by agreement); ✓✓Development of new product formulations in accordance with market needs and with “added value” that separates you from competing products; ✓✓Attentive customer-oriented approach, tailored to your needs, with professional and timely service, competitive time to market (TTM) and fast response time to all your inquiries.

Nutramedic &Cosmetics

Photoprotective effect of antioxidants on the skin Although the skin naturally contains antioxidants, their amount becomes limited during sun exposure. Protective agents applied to the skin have their limitations in protecting the skin, and dietary supplements can help compensate for these deficiencies.

he skin is the largest body organ that plays the role of a biological barrier. It participates in the regulation of body temperature, transmits sensations and prevents harmful substances from entering the body. One of its most important protective functions is to protect against the undesirable effect of solar radiation.

The impact of sunlight on the skin

Sunlight is beneficial for the human body, and one of benefits is stimulation of formation of vitamin D in the skin, which is essential for healthy bones and immunity and increases serotonin level that supports a good mood. But excessive sun exposure, both short and long term, can damage the skin. Besides causing burns, premature skin aging, hyperpigmentation and allergic reactions, it can even lead to serious conditions such as skin cancer. Exposing the skin to the Sun’s UV radiation leads to significant oxidative damage resulting in clinical and histopathological changes, thus contributing to its premature aging. Hyperpigmented lesions, also known as “age spots“, are some of the most noticeable skin changes caused by photoaging. The vast majority of skin damage caused by the Sun results from UV rays, namely UVA and UVB rays. The skin naturally contains an antioxidant system against UV-generated reactive oxygen metabolites. However, this antioxidant protection is not always fully effective during Sun exposure. Oral antioxidants can help reduce the harmful effects of UV radiation and strengthen the physiological antioxidant defences of the skin. Various ways of protecting against UV rays include photo protective clothing and applying topical agents that require repeated application due to localized effect. The introduction of useful oral substances is a promising method, providing uniform protection to the whole body. This has led to the development of a market for dietary supplements and products promoted to improve skin health. All these products have in common antioxidant action.

AUTHOR:

Daria Šurić, M.Pharm., Darmell

Simply explained, antioxidants “fight” against the oxidation process, which over time leads to the destruction of cells. Since oxidative stress is considered to be the main cause of skin aging, nutrients that have antioxidant effects such as carotenoids, flavonoids, plant extracts and omega-3 fatty acids are of interest. Most UV radiation is filtered through the Earth’s atmosphere, and we are exposed mainly to UVA (95%). Primarily, research has focused on the effects of UVB rays, but lately attention has been more shifted to UVA radiation. Its larger wavelengths allow it to penetrate deeper into the skin. Majority of UVB is absorbed in the epidermis, and UVA radiation also affects the dermis, showing carcinogenicity in animal models. UVB rays can also cause burns. Clinical changes observed in photo aging such as rough structure, wrinkles, spotty hyperpigmentation, vasodilation and loss of elasticity are attributed primarily to the influence of UVA. UVA harms the skin by stimulating the formation of reactive forms of oxygen after they are absorbed by riboflavin, porphyrin and hem-containing proteins. Reactive oxygen molecules can damage import-

Nutramedic &Cosmetics ant biomolecules including DNA, proteins and lipids in various parts of the skin, leading to cytotoxicity, mutations and changes in signalling pathways in cells. UVB rays reduce the effectiveness of antioxidant enzymes at the protein level, leading to the accumulation of reactive oxygen radicals (ROS). Since the harmful effects of the sun on the skin provoke oxidative stress, i.e. the formation of free radicals, one way to protect the skin is within, using dietary supplements containing antioxidants. The human body depends on antioxidants that help maintaining balance and healing damaged cellular structures. Although the skin itself naturally contains antioxidants, during Sun exposure their amount becomes limited. Protection with the products applied to the skin also has its limitations due to e.g. inadequate application (too small amounts, insufficient frequency) or rinsing by sweating. Dietary supplements can help compensate for these shortcomings. Their action is preparing the skin for sun exposure and helping to eliminate the formed free radicals. Regarding active substances found in the aforementioned dietary supplements, we will describe in more detail several, namely those that have a proven photoprotective effect on the skin.

Astaxanthin

Astaxanthin belongs to the group of carotenoids found in nature. It is also called the “king of carotenoids” because it is one of the strongest natural antioxidants. Its rich burgundy pigment gives color to krill, salmon and shrimps. There are different sources of astaxanthin (food, bacteria, synthesized and natural from microalgae) that can also have different bio-availability. The best source of astaxanthin for dietary supplements is obtained from a natural source, the microalgae Haematococcus pluvialis, which contains about 40 grams of astaxanthin per kilogram. For comparison, salmon meat contains 5 mg of astaxanthin per kilogram. Nowadays, astaxanthin from microalgae can be produced in industrial quantities. Microalgae produce it to protect against the stressful influences of the environment. Hence, when the drying out begins during the summer, it allows them to preserve DNA from damage. In this way, they remain ready for regrowth when the rains come, and even if it will take several years. This astonishing ability shows the effectiveness of astaxanthin. It manifests its antioxidant action by collecting free radicals. Its ability to eliminate free radicals has been proven to be 1,000 times greater than that of coenzyme Q10 (Mori J.) A wide range of clinical studies in several different health areas has proven its multiple positive effects on the human body, in the following areas: • joint and tendon health; • skin health; • cardiovascular health; • eye and brain health; • protection at the cellular level; • recovery after sport activity.

rocytes to the brain where it crosses the bloodbrain barrier, and also into the skin, including the dermis and epidermis. This unique antioxidant is bioavailable throughout the organism. In addition to the skin, it can be found in the muscles, ligaments, tendons, eyes, cardiovascular system, nervous system and internal organs. These soft tissues work better thanks to the presence of the antioxidant power of astaxanthin. The human organism cannot synthesize it on its own, so it is necessary to introduce it in food and dietary supplements. The protective role of astaxanthin on the skin concerning damage caused by UV radiation has been proven on cell cultures, rodents and in humans. Taking astaxanthin prevented UV-induced photo aging and improved the healing of burns in rodents (Komatsu T.). In addition to astaxanthin, several other carotenoids, such as beta-carotene and lycopene, showed a protective role in UV-induced skin damage (Sies H.). In a randomized, double blinded, placebo-controlled clinical trial (Ito N.), 23 healthy individuals were divided over 10 weeks into a group that was given 4 mg of astaxanthin in capsules and into a placebo group. To prove the protective role of astaxanthin on UV-induced skin damage, a minimum erythema dose (MED) was established and UV-induced transepidermal moisture loss (TEWL) was analysed at the beginning and after 9 weeks of supplementation. Thanks to the results achieved, it has been proven that astaxanthin acts protectively on UV-induced skin aging and helps maintaining healthy skin in healthy individuals. Side effects or significant changes in biochemical parameters in the blood and hematological tests were also monitored. Side effects associated with taking astaxanthin have not been observed. Impact of astaxanthin supplementation on minimal erythema dose

FIGURE 1

Astaxanthin is absorbed by passive diffusion from the small intestine, transported to plasma and eryth-

Nutramedic &Cosmetics Dietary supplements with astaxanthin increase the minimum erythema dose (MED). (a) Image of the irradiated area in the placebo group (left) and astaxanthin group (right) before and after supplementation; (b) Changes in the MED baseline in the placebo group (black) and astaxanthin group (red). * p < 0.05 with Mann-Whitney U-test. Error bars indicate a standard deviation (SD). Taken from: The Protective Role of Astaxanthin for UV-Induced Skin Deterioration in Healthy People-A Randomized, Double-Blind, Placebo-Controlled Trial - PubMed (nih.gov) In dietary supplements on Croatian market, doses of astaxanthin range from 4 to 8 mg. According to EFSA, a daily intake of up to 8 mg is safe for adults even in combination with a high intake of astaxanthin from food (salmon, shrimps). For adolescents up to 14 years of age and younger children are it is recommended 4 mg per day (EFSA Journal). Astaxanthin has a unique molecular structure among carotenoids. It has hydroxyl and keto parts of the molecule, which allows it to stretch through the two-layer cell membrane from the outer wall to the inner side, and also to eliminate oxidized fats from the surface of the membrane. It is a lipophilic molecule that is well absorbed when taken with food. One of the leading manufacturers of astaxanthin is the company Algatech. They have developed a protected process of cultivation of microalgae, which lasts approximately three months and mimics the accumulation of astaxanthin in natural conditions. Cultivation begins in the “green stage”. Algae are provided with optimal growing conditions and biomass is allowed to grow to its maximum over time. At a later stage, algae are transferred to advanced glass photobioreactors where they grow rapidly under the Sun of the Arava Desert. When fully developed, the “red stage” begins, in which algae are subjected to stressful conditions that sample the natural response - the production of astaxanthin for protection. This method of production ensures high purity of astaxanthin, with more than 95% of total carotenoids. Nutrex Hawaii is also a world-renowned manufacturer of astaxanthin, founded by Dr. Gerald Cysewski, an expert in microalgae and carotenoid, in Hawaii in 1990. Their production is located on the unpolluted Kona Coast in Hawaii, where natural resources are used in production. Studies have proven that after two weeks of consumption of this astaxanthin, the time it takes to form a burn caused by exposure of the skin to the Sun is significantly extended. The inflammatory reaction of the skin and subcutaneous tissue, which arises as a reaction to excessive exposure to UV radiation, is also reduced.

Other carotenoids

Beta-carotene belongs to the group of carotenoid micronutrients found primarily in fruits and vegetables. Besides the specific antioxidant effect, they also act on impulse transmission and gene expression at the cellular level. Together with lycopene, beta-carotene is a pigment that gives color to carrots and tomatoes and they are the dominant carotenes in the blood and tissues as well. Both substances affect the skin resistance when taken as a

FIGURE 2

Haematococcus pluvialis

TAKEN FROM: https://www.nutrex-hawaii.com/blogs/learn/

what-is-astaxanthin

dietary supplement. They have been proven to protect the skin from burns caused by solar radiation (solar erythema) by increasing the basal ability to protect the skin from UV damage (Stahl W.). Data collected by in vitro studies show that other carotenoids also have a photoprotective effect. One of them is lutein. For successful carotenoid driven protection of the skin, it is recommended to take them at least ten weeks. Increased carotenoid intake can help protecting the skin from UV radiation. Beta-carotene is a precursor of vitamin A, converted into vitamin A in the liver. Natural beta-carotene increases skin hydration. Vitamin A was approved to contributing to the maintenance of normal skin. In one study, 30 healthy male volunteers were given beta-carotene at a dose of 180 mg/day, or placebo during 10 weeks, followed by exposing to sunlight in the Arizona desert for 2 hours. Beta-carotene had a small but statistically significant effect, by increasing the minimal erythema dose (MED) of solar radiation (Mathews-Roth MM). In another, placebo-controlled study, in which 30 mg/day of beta-carotene was used for 10 weeks, a significant decrease in the intensity of Sun-induced erythema (Gollnick H.P.M.) was observed. Dunaliella salina, a microalga that lives in the oceans, produces a large concentration of beta-carotene and other carotenoids to protect against intense UV radiation. Similarly, beta-carotene exhibits an antioxidant effect on the human body and protects the skin from the Sun. Dunaliella salina is a natural source of other mixed carotenoids, cryptoxanthin, zeaxanthin and lutein. It is considered as one of the highest quality sources of beta-carotene for use in dietary supplements. It is important that people who have sensitive skin prone to rashes, eczema or psoriasis increase their intake of beta-carotene to increase skin protection from the Sun. Beta-carotene is best taken in its natural form (along with other carotenoids) for at least 12 weeks. Lutein and zeaxanthin - Lutein is a stereoisomer of zeaxanthin, also belonging to the carotenoids. Its role in preserving healthy vision is well known, while in protecting the skin it is somewhat less mentioned. Lutein is also found in the skin, in the epidermis and dermis. It contributes to its color and protects it by

Nutramedic &Cosmetics blocking blue light. In this way, it reduces the appearance of melasma, a pigmentation disorder that manifests itself as the appearance of dark spots, especially facial (Souyoul SA; Balić A.). Lutein also manifests its protective role from blue light on the retina of the eye by filtering blue light and acting as an antioxidant. To protect the skin, recommended dose is 10-20 mg/day. It is often combined with zeaxanthin. Since lutein is fat-soluble, like other carotenoids, taking a dietary supplement is recommended with a meal. Regarding well-known brands found as raw materials in food supplements, Omniactive Lutemax 2020 lutein® and zeaxanthin and Kemin FloraGLO® lutein are often mentioned. FloraGLO® lutein is a natural ingredient derived from marigold flower (Tagetes ere cta), and has results from 25 years of research. It’s the most tested lutein brand in the world. It has excellent absorption, since it contains a form of lutein equal to that of vegetables and other foods. Lutemax 2020® is an ingredient that contains premium lutein and zeaxanthin isomers in a ratio of 5:1, as found in nature. Red orange extract Red orange (Citrus sinensis var Moro, Tarocco and Sanguinello) has great healing potential. Namely, in fruits, leaves, juice and roots it contains a whole wealth of active molecules that contribute to pharmacological activity: flavonoids, steroids, coumarins, peptides, carotenoids and nutrients such as potassium, magnesium, calcium and sodium. Proving the in vitro antioxidant effect, it was assumed that it has also a photoprotective effect. A study was conducted by supplementation with red orange extract in relation to photoprotective action in UV-induced skin erythema and in relation to the production of melanin in solar lentigo. The test was done on healthy volunteers using the appropriate instrumental method. The dose was 100 mg of extract per day, for 15 days. A significant decrease in the degree of erythema was shown, and pigmentation spots on the skin (melanin content) decreased from 27% to 7%. It was concluded that a dietary supplement containing red orange extract can increase the antioxidant protective capacity of the skin, protecting it from the harmful processes involved in photo aging thereby improving its appearance and pigmentation (Puglia C.). FIGURE 3

Chemism of the red orange fruit

Fatty acids

Important factors for skin health are fatty acids found in unrefined Evening primrose, Borage and Hemp (GLA) oils, and blue fish and algae (omega-3). Fatty acids play a key role in creating the skin lipid barrier and affect the response of skin cells to UV radiation. Omega-3 fatty acids also showed photoprotective action at a dose of 2.8 g of EPA and 1.2 g of DHA during 4 weeks (Orengo IF). The mechanism of action is associated with anti-inflammatory properties so that it reduces the UV-induced release of cytokines and other mediators from different types of skin cells.

Polyphenols

Polyphenols are antioxidants present in fruits, vegetables, cereals, olive oil, chocolate, but also in beverages like coffee, tea and wine. Polyphenolic compounds suppress local and systemic immunosuppression caused by UVB rays, and mediate the reduction of reactive forms of oxygen in the skin. Green tea - Epigalocatechin-3-gallate (EGCG) is the most prevalent polyphenol in green tea, being primarily responsible for its positive effects on health. EGCG has two triphenol chemical groups in its composition. These groups are significant for the antioxidant effect that also protects against UV-induced DNA damage. People who do not tolerate well large amounts of green tea, it is recommended to take dietary supplements containing its extract, standardized to the content of EGCG.

Quercetin

Quercetin is a flavonoid native to vegetables, fruits or ginkgo. In several studies, he has shown anti-inflammatory and antioxidant properties. In the skin, it prevents the breakdown of collagen induced by UV radiation. Quercetin also affects the slowdown of skin photo aging, supports growth and prolongs the life of skin cells, and fibroblast resistance.

Resveratrol

Resveratrol is a substance found in red wine, associated with its positive effects ion health. It is found in the fruits of grapes, more precisely in the skin and seeds of the fruit, as a protection against toxins. Red grapes are fermented together with the skin so that they contain resveratrol. It is also found in white wine, but in an amount 10 times less than in red. Like other bioflavonoids, it has a proven antioxidant effect (Brasnyó P.).

Polypodium leucotomos extract

SOURCE: Molecules | Free Full-Text | Chemistry and Pharmacology of

Citrus sinensis (mdpi.com)

Fern extract Polypodium leucotomos has been used in folk medicine of South American Indians to treat skin inflammation since ancient times. It has an antioxidant effect that has been proven by clinical trial during which a significant decrease in erythema has been observed. The conclusion of the conducted research is that oral intake of a dietary supplement containing the extract of this fern has a chemophotoprotective effect, thus exhibiting a significant ability to protect

Nutramedic &Cosmetics the skin from UV radiation (Middlekamp-Hup MA). Among other ingredients that could play a significant role in dietary supplements to protect the skin from solar radiation, oral intake of hyaluronic acid shows promising potential. Overall, conclusion is that taking described substances in a form of food supplements has significant beneficial effect on protection of the skin from harmful impact of the Sun radiation.

References:

Mori J., Yokoyama H., Sawada T., Miyashita Y., Nagata K. Anti-oxidative properties of astaxanthin and related compounds. Mol. Cryst. Liq. Cryst. 2013;580:52–57. doi: 10.1080/15421406.2013.804380. [CrossRef] [Google Scholar] Ito N, Seki S, Ueda F. The Protective Role of Astaxanthin for UV-Induced Skin Deterioration in Healthy People-A Randomized, Double-Blind, Placebo-Controlled Trial. Nutrients. 2018 Jun 25;10(7):817. doi: 10.3390/nu10070817. PMID: 29941810; PMCID: PMC6073124. Satoh A., Mitarai A., Kanno T., Hori Y., Takeda R. Effects of the Intake of Astaxanthin on the Reduction of Skin Darkling Induced by UV Irradiation in adult women. Pharmacometrics. 2011;80:7–11. Komatsu T., Sasaki S., Manabe Y., Hirata T., Sugawara T. Preventive effect of dietary astaxanthin on UVA-induced skin photoaging in hairless mice. PLoS ONE. 2017;12:e171178. doi: 10.1371/journal.pone. 0171178. [PMC free article] [PubMed] [CrossRef] [Google Scholar] Sies H., Stahl W. Carotenoids and UV protection. Photochem. Photobiol. Sci. 2004;3:749–752. doi: 10.1039/b316082c. [PubMed] [CrossRef] [Google Scholar] https://efsa.onlinelibrary.wiley.com/doi/10.2903/j.efsa.2020.5993 Stahl W, Sies H. Photoprotection by dietary carotenoids: concept, mechanisms, evidence and future development. Mol Nutr Food Res. 2012 Feb;56(2):287-95. doi: 10.1002/mnfr.201100232. Epub 2011 Sep 23. PMID: 21953695.

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Mathews-Roth MM, Pathak MA, Parrish J, Fitzpatrick TB, Kass EH, Toda K, Clemens W. A clinical trial of the effects of oral beta-carotene on the responses of human skin to solar radiation. J Invest Dermatol. 1972 Oct;59(4):349-53. doi: 10.1111/1523-1747.ep12627408. PMID: 4569104. Gollnick H.P.M., Hopfenmuller W., Hemmes C., Chun S.C., Schmid C., Sundermeier K., Biesalski H.K. Systemic beta-carotene plus topical UV-sunscreen are an optimal protection against harmful effects of natural UV-sunlight: results of the Berlin-Eilath study. Eur. J. Dermatol. 1996;6:200–205. [Google Scholar] [Ref list] Souyoul SA, Saussy KP, Lupo MP. Nutraceuticals: a review. Dermatol Ther (Heidelb). 2018;8(1):5–16. doi:10.1007/s13555-018-0221-x [PMC free article] [PubMed] [CrossRef] [Google Scholar] Balić A, Mokos M. Do we utilize our knowledge of the skin protective effects of carotenoids enough? Antioxidants. 2019;8(8):259. doi: 10.3390/antiox8080259 [PMC free article] [PubMed] [CrossRef] [Google Scholar] Puglia C, Offerta A, Saija A, Trombetta D, Venera C. Protective effect of red orange extract supplementation against UV-induced skin damages: photoaging and solar lentigines. J Cosmet Dermatol. 2014 Jun;13(2):151-7. doi: 10.1111/jocd.12083. PMID: 24910279. Orengo IF, BlackHS, WolfJE. Influence of fish oil supplementation on the minimal erythema dose in humans. ArchDermatolRes1992; 284:219–21. Brasnyó P, Molnár GA, Mohás M, Markó L, Laczy B, Cseh J, Mikolás E, Szijártó IA, Mérei A, Halmai R, Mészáros LG, Sümegi B, Wittmann I. Resveratrol improves insulin sensitivity, reduces oxidative stress and activates the Akt pathway in type 2 diabetic patients. Br J Nutr. 2011 Aug;106(3):383-9. doi: 10.1017/S0007114511000316. Epub 2011 Mar 9. PMID: 21385509. Middelkamp-Hup MA, Pathak MA, Parrado C, Goukassian D, RiusDíaz F, Mihm MC, Fitzpatrick TB, González S. Oral Polypodium leucotomos extract decreases ultraviolet-induced damage of human skin. J Am AcadDermatol. 2004 Dec;51(6):910-8. doi: 10.1016/j.jaad. 2004.06.027. PMID: 15583582.

Nutramedic &Cosmetics

INNOVATION & INSPIRATION

Fi & Hi Europe set to attract over 20,000 global attendees

i Europe co-located with Hi Europe returns to France this year from 6-8 December. For the first time, the leading ingredients exhibition will take place at Paris Expo Porte de Versailles – occupying four halls with more than 1,200 exhibitors, ranging from leading ingredients suppliers through to highly specialized newcomers. The in-person show will be framed and supported by an extensive online event, giving attendees countless opportunities and greater flexibility to source, connect and innovate – be it virtually, in-person or both. In Paris, Fi & Hi Europe is set to attract exhibitors and attendees from over 135 countries around the world. Located in the heart of the city, Paris Expo Porte de Versailles is within walking distance of many hotels, restaurants and bars – making the event more visitor-friendly than ever before. Fi & Hi Europe is the culmination of a year of Fi Global engagement with the ingredients industry, including a series of webinars, in-person events around the world, and the marketplace solution Ingredients Network. Digitally enabled event concept With this year’s show, Informa Markets continues its hybrid journey, setting the bar even higher with more advanced technology. The combination of online and inperson events offers attendees the best of both worlds, covering a huge variety of live and on-demand content, as well as data-driven matchmaking options, enabling a seamless all-round show experience. For example, all exhibitors will have an online profile that allows them to connect and network ahead of the event and gather leads onsite using the integrated lead scanning system. This means it has never been easier to find and connect with relevant contacts and schedule virtual or in-person meetings using the online event platform. “We’re looking forward to welcoming thousands of visitors and exhibitors from around the world to an event which all those involved in the ingredients industry simply cannot afford to miss,” says Julien Bonvallet, Brand Director at Informa Markets. “After many months of disruption and uncertainty, there’s a strong aspiration for meeting in-person again, and so the excitement is already gathering pace. But for those who would prefer to join online, sophisticated technology means our online event will merge seamlessly with the in-person show.” Packed conference programme The online event will begin on 28 November, the week before doors open at Paris Expo Porte de Versailles. It features a packed conference programme covering the hottest industry topics including plantbased, health & wellbeing and reformulation, as well as category updates on some of the key trending

segments. These include bakery and snacks, dairy & dairy alternatives, sustainability and much more. The online event will begin on 28 November, the week before doors open at Paris Expo Porte de Versailles. It features a packed conference programme covering the hottest industry topics including plantbased, health & wellbeing and reformulation, as well as category updates on some of the key trending segments. These include bakery and snacks, dairy & dairy alternatives, sustainability and much more. Unique gathering of suppliers Lots of key ingredients suppliers have already confirmed their attendance in Paris, including ADM, Beneo, Brenntag, Cargill, DSM and Ingredion, to name just a few. The four halls at the venue are already 85 per cent booked with a variety of exhibitors – both old and new – all eager to showcase the latest trends and products driving the industry. There will also be more country pavilions than ever before. Julien adds: “I am so excited that Fi & Hi Europe will once again open its doors in Paris – this time at an impressive new venue. Bringing the world of food and beverage ingredients together has always been our main purpose, and our entire team is looking forward to providing the perfect platform for both visi tors and exhibitors to source, connect and innovate.” For more information on the show, visit: https:// www.figlobal.com/fieurope/en/home.html

Fi Global Food ingredients Global was launched in Utrecht, The Netherlands, in 1986. Its portfolio of live events, extensive data, digital solutions and high-level conferences is now established throughout the world and provides regional and global platforms for all stakeholders in the food ingredients industry. Over 500,000 people have attended our shows over the years, with billions of Euros worth of business created as a result. With more than 30 years of excellence, the events, digital solutions and supporting products deliver a proven route to market, with a truly global audience. Since 2018 Food ingredients Global has been part of the Informa Markets portfolio. For more information about, please visit: www.figlobal.com.

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