The use of estuarine environmets by Epinephelus itajara: a non-lethal approach Lorena Lopes Almeida, Tainara Fonseca Simões, Lainny Avelar, Marcos Santos Bomfim, Ana Paula Cazerta Farro, Maurício Hostim Silva Federal University of Espírito Santo - Postgraduate Program in Biological Sciences - PPGBAn. E-mail: lorenalopesbio@gmail.com
Epinephelus itajara Mero, canapú, bodete, badejão, goliath grouper;
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Inhabits marine and brackish waters, associaciates with complex
Samples
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Research & Conservation 2012 • 218
2015 • 71
2018 • 31
and hard substrates and shows strong site fidelity; •
Fieldwork
Large sized, late sexual maturity, long life, slow growth, spawning aggregations;
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Critically Endangered (IUCN Red List);
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The first marine fish subjected to a strict moratorium in Brazil;
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Non-lethal sampling.
Epinephelus itajara
Environmental Education
Brazilian Coast
Stable Isotope Analysis Drying oven 4h (55° C)
Hydrogen Peroxide (30%/5min)
Cross Section 1.5mm
Life Time Series (Bands)
Combustion 1.050°C
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Habitat use by juveniles (δ13C e δ15N);
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Basal resources (δ13C);
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Trophic level changes between the ontogenetic phases (δ15N);
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The trophic level (δ15N): each site and between sites.
Genetic Analysis
DNA Extraction Quantification
Mitochondrial markers
Cyt b D-Loop
Microsatellite markers
10 loci PCR Amplification
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Individual genotyping;
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Sibship reconstruction;
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Genetic diversity;
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Gene flow;
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Population structure.
PCR Amplification
DNA Sequencing
Genotyping
DNA Fragment lenghts