The Chemistry of Photosynthesis
light energy water + carbon dioxide
glucose + oxygen
Photosynthesis is the process by which organic molecules are synthesised by the reduction of carbon dioxide. The energy required for this comes from light energy. Light energy is absorbed by photosynthetic pigments.
Photosynthesis consists of two parts: • A Light-dependant stage called photolysis • A Temperature-dependant stage called carbon-fixation or The Calvin Cycle
Photolysis
Photolysis
Light energy is used to spit molecules of water into hydrogen and oxygen. This happens in the grana of choloroplast
Light energy splits water into hydrogen and oxygen – “photolysis” Hydrogen combines with a hydrogen acceptor NADP to form NADPH2
Energy released regenerates ATP from ADP and Pi – “photophosphorylation” The hydrogen carried by NADPH and 2 the energy held by ATP are essential for carbon-fixation
Top Tip and Handy Hint NADP is the hydrogen acceptor in Photosynthesis, don’t confuse with NAD in respiration
What you should know...
Carbon Fixation This temperature-dependant stage of photosynthesis consists of several enzyme-controlled reactions forming a cycle called the Calvin Cycle.
Unstable 6C compound
Glycerate Phosphate (GP) 3C NADPH2
CO2
NADP
(1C)
Triose phosphate (3C)
Ribulose Bisphosphate (RuBP) 5C
Glucose (6C)
Carbon dioxide entering the chloroplast combines with 5-carbon ribulose bisphosphate (RuBP) Occurs in the Stroma This molecule is unstable and quickly splits into two 3-carbon molecules of glycerate-3-phosphate ATP and hydrogen (in NADPH ) from 2 photolysis are used to produce 3carbon triose phosphate sugar Some of this is then converted to glucose (6 carbons)
Some triose phosphate are used to regenerate RuBP Conversion of triose phosphate (3C) to RuBP (5C) requires energy from ATP
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We need to know the number of carbon atoms in the key molecules: Key Molecule Carbon dioxide GP RuBP Glucose
Number of Carbon Atoms
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We need to know the number of carbon atoms in the key molecules: Key Molecule
Number of Carbon Atoms
Carbon dioxide
1
GP
3
RuBP
5
Glucose
6
Summary:
Rate of photosynthesis The rate of photosynthesis can be calculated by measuring: Rate of oxygen production (counting bubbles) Rate of CO uptake 2
Production of carbohydrate (increase in dry mass)
Limiting Factors = a factor that limits the rate of a reaction by being in short supply The rate of photosynthesis can be limited by: Light Intensity Carbon Dioxide concentration Temperature
e.g.1 Plant kept in low light intensity (Graph ABC):
Between points A and B, rate of photosynthesis is limited by CO2 concentration Between points B and C, rate of photosynthesis is limited by light intensity
e.g.2 Plant kept in higher light intensity (Graph ADE):
In Graph ADE, CO2 concentration is the limiting factor for a greater part of the graph (A-D, compared to A-B at low light intensity) Light intensity only becomes the limiting factor at point D