general products
11 Issue 3 2020
Real-time PCR assay test for Salmonella quantification
H
ygiena, a global leader in food safety technology, offers the BAX SalQuant system - a method that uses a real-time PCR assay with Hygiena’s BAX Q7 system, to accurately quantify the amount of Salmonella. This application can be utilised throughout the beef, pork and poultry industries to provide the precise amount of Salmonella. The BAX real-time SalQuant PCR assay test kit is the first to receive AOAC-RI approval. Getting more accurate results For the first time ever, there is a rapid method that can provide quantitative data on contamination levels by performing real-time PCR on ground chicken and turkey collected from a food production facility. After only eight hours of enrichment, samples are analysed using the BAX SalQuant system. The resulting cycle threshold (CT) values for positive samples is utilised to quantify the number of colony-forming units (CFUs) present in the sample. No additional kits or components are required, only the BAX system RT PCR assay for Salmonella. This allows the collection of quantitative, numeric data on
contamination levels that is location- or matrix-specific, more accurate and precise, and less laborious than MPN (Most Probable Number). By combining the power of realtime PCR, the BAX system RT assay for Salmonella, and available protocols for multiple sampling locations throughout poultry production, processors can now quantify Salmonella from the farm to final product to improve and verify sanitation and antimicrobial intervention processes, to meet more stringent regulatory and quality standards, and protect consumers by knowing the risk a positive sample could pose. Processors can more quickly and confidently respond to increasing Salmonella contamination issues, allowing them to better focus their sanitation and antimicrobial interventions to root causes, saving money that otherwise would be used for wider interventions. Powerful PCR detection Hygiena’s BAX systems offer powerful PCR detection technologies using real-time and end-point assays which are validated for a wide variety of food and environmental matrices. BAX systems are automated and
configured for convenience, minimising operator handling and improving ease-ofuse. Systems provide all the necessary components for PCR - polymerase, nucleotides and target-specific primers with tableted reagents and optimised media.
MICROSEP (PTY) LTD Frances Renwick Tel: (011) 553 2300 Fax: (011) 553 2400 frances.renwick@microsep.co.za www.microsep.co.za
Endecotts – a world standard in particle size analysis equipment. The Endecotts Minor 200 ,Octagon 200, Octagon 200CL and the AirSizer 200 have all been revamped and revised and especially developed to accommodate the exacting requirements of the modern laboratory. Ergonomically designed and with a fresh look, Endecotts Sieving machines are ideal for your busy Laboratory. Airsizer 200
Instrulab – a Division of Air & Vacuum Technologies (Pty) Ltd – are the sole agents for the Endecotts range of products for Southern Africa.
Minor 200 Octogon 200
info@vactech.co.za
Octogon 200 (wet)
A&VT = ADDING VALUE TOGETHER
SALES, SERVICE & REPAIRS
011 318 3241/2/3/4
www.vactech.co.za
