ISSUE 5 2011
ESTABLISHED IN AMSTERDAM, 1985
18+ For adults only. Soft Secrets is published six times a year by Discover Publishers USA, Inc.
In this Issue: MOJAVE MAMA The desert isn’t the easiest place to grow weed, but it can be done. Mojave Mama and her tales of triumph might even inspire you to go out and get involved within your own community... ›› 12
Harvest Season
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UNFRIENDLY INVADERS Despite all our best efforts, infestations are an unfortunate part of gardeners’ lives. An organic expert teaches you how to deal with the most common pests in a safe and thorough manner.. ›› 17
Hemp Nutrition Double Feature
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DIY HEMP SKINCARE Hemp cosmetics and toiletries are so good for our skin, but often priced above many people’s budgets. Learn how to make your own! ›› 29
Mapping the Cannabis Genome On August 18th 2011, the Massachusettsbased company Medicinal Genomics released the raw sequence of the newly mapped Cannabis sativa genome, and is set to release the C. indica genome in a matter of weeks. This historic event is set to have far-reaching implications for the field of medicinal Cannabis research, as well as opening up the possibility of far more accurate quality control testing – and giving breeders a huge advantage in the drive to create new strains.
released for public use through Amazon’s EC2 cloud computing service. However, future funding is unlikely to be an issue as the interest in this project – and its results – has been phenomenal; the number of potential applications for various sectors is countless, and much more work must be done to interpret the raw data into a precise genomic structure. Having made such an initial impact, this energetic young company is sure to be making genomic headlines again in the future.
cies, DNA forms chromosomes containing both genes and non-coding DNA. A gene is a stretch of DNA that controls a hereditary trait in a species; an allele is the expression or variation of that gene in individuals of the species. Non-coding DNA may represent a large proportion of the genome, but is not encoded as genes and not all of its functions are fully understood. The number of chromosomes in the genome differs between species: humans have fortysix, Cannabis just twenty; the number of genes within the chromosome also varies. DNA itself is a biological polymer or repeating macromolecule, made up of millions of sections called nucleotides. These sections are comprised of a simple sugar, a group of phosphates, and a
The study of genetics is a complex business in itself, and at first glance appears incomprehensible for most. The majority of people vaguely remember the basics from high-school biology class, but would be hard-pressed to provide a decent explanation. SSUSA brings you up to speed, while going into a little more detail about the Cannabis genome project itself and why its impact on Cannabis research will be so great. Kevin McKernan, CEO of Medicinal Genomics, has a strong background in the development and implementation of sequencing technology. He was involved with the Human Genome Project and was responsible for the design of a new bench-top SOLiD sequencing instrument during his time at Life Technologies, a highly regarded biotechnology company. With Medicinal Genomics, he was able to combine his own expertise and the unique opportunity presented by the emerging medical Cannabis market to begin his own concern. It is estimated that the project cost around $200,000 in total, a cost that Kevin evidently does not seek to recoup through licensing use of the data, as it has been
It might be pertinent at this point to provide a short introduction to genomics. Put as succinctly as possible: genomics is the field of genome research, and is a highly specialized branch of genetic studies. The genome is the complete genetic code of which an individual is comprised, and is contained within the DNA. In most spe-
nitrogenous base (nucleobase). This base may be one of four different compounds – adenine, guanine, thymine and cytosine. Through billions of permutations of these four bases, every living species has evolved its own unique genome. Continues on page 5