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The UK’s leading equine health & well-being magazine
May/June 10 £2.95
Researchers on brink of DNA breakthrough By Louise Cordell RESEARCHERS are on the brink of finalising a DNA profiling technique that will be able to confirm the identity of racehorses that have tested positive for drugs. It is hoped that this breakthrough will reduce hearing costs when samples are contested as well as increasing public confidence in the industry. The study has been funded by the Australian Government’s Rural Industries Research and Development Corporation and has shown that urine samples can be used to unequivocally identify a horse using DNA profiling. This has been impossible in the past as urine only contains small levels of DNA, however the researchers are now closer to a reliable test that could be used by drug testing laboratories. The report, ‘DNA Profiling of Horse Urine Samples to Confirm Donor Identity’, is based on work carried out by Paula Hawthorne and colleagues at the University of Queensland. The team tested a range of profiling techniques on seven urine samples and compared the results with hair samples from the same horses. DNA profiling is routinely used to prove identity and parentage in horses, but the process is usually based on hair rather than urine because urine only contains a small
amount of cellular material, and the DNA that is present degrades rapidly. Their results showed that both storage time and temperature had a significant effect on the success of the DNA profiling. To be of use, urine could be stored at 4°C for no more than two days (or frozen at 20°C or -80°C) before processing, and samples stored at 4°C for a week or more resulted in no useable results. The most successful results were achieved using a commercially available test, which allowed the researchers to identify all twelve DNA ‘microsatellite markers’, in four urine samples – all from male animals. These profiles from the urine samples also matched the DNA profile from the respective hair sample from the same horse. Despite the successful results of the study, there is still more work to do. The researchers have now made a series of recommendations pointing out that once the best profiling method has been established, more tests will be needed to find out if the results are affected by various drugs, and also to validate and refine the procedure for routine operations. However, they confirm that once the optimum method has been finalised, it should not be long before it can be integrated into existing routines at racing drug-testing laboratories.
PERMISSION has been granted for a 50m high white horse to be built beside the A2 dual carriageway in Kent. Gravesham Borough Council gave the goahead for the Ebbsfleet Landmark Project – a thoroughbred racehorse 33 times larger than a real horse – created by artist Mark Wallinger. The aim of the scheme is to create a high profile marker for the Ebbsfleet Valley which will be
visible from Eurostar trains and the search is now on to find potential funding partners. Mark Wallinger said: “This is a tremendously exciting project. I was honoured that my White Horse won and I am delighted that the council has granted it planning permission. The team and I are very much looking forward to creating a new landmark for the area.” Picture: Anthony Reynolds Gallery, London