A Bit About Us
Thorlabs’ Mascots Since its inception in 1989, Thorlabs has developed a series of customer facing “mascots,” in the form of dogs, which represent our attitude and focus toward serving the photonics and life science communities. Many customers wonder why a dog was chosen as our main image and not a hammer or some other depiction of the Norse God Thor. The answer is that the company’s namesake is that of owner and founder, Alex Cable’s canine companion growing up, a Labrador retriever named Thor. The first piece of dog artwork, “Dog Eat Dog,” a painting by artist Peter Marco, was discovered by Cable during a trip into New York City. An artist himself, Cable appreciated the bold and slightly wacky look, purchasing it as well as its successor, “Hot Dog,” the second piece of dog artwork used by Thorlabs. After using Hot Dog for several years, Thorlabs adopted “Born to Serve,” the first of a series of dogs created to truly characterize Thorlabs’ goal within the industry: to serve customers’ needs by delivering products as quickly as possible, a strength the company has built upon through an extensive portfolio and same-day shipping on stocked items. At the same time Born to Serve was revealed, Thorlabs also launched “All Ears,” whose namesake is another testament to one of our core principles. Customer feedback is inherently important to the success of Thorlabs and our partnership with the photonics and life science communities. All Ears is a symbol of our commitment to offer an open dialogue and partnership with all our customers. Upon retiring Born to Serve, Thorlabs adopted its current dog and mascot, “Face Value,” whose name reflects our open and transparent stance in the market and towards our customers. Face Value has become a familiar icon through both the photonics and life science industries, appearing on hats, bags, water bottles, and t-shirts. Face Value stands to remind Thorlabs employees and customers alike that we are a customer-centric organization dedicated to offering service and support through a low-friction interface.
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Electrophysiology Selection Guide
Cerna Series Microscopes
Rigid Stands
Pages 270 - 273
Pages 296 - 300
Patch-Clamp Manipulators
Faraday Cage
Pages 274 - 279
Pages 301 - 303
Physiology Stages
EMF Shielding
Pages 280 - 293
Pages 304 - 305
Motorized Platforms Pages 294 - 295
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Electrophysiology Cerna Series Microscopes
Overview: Cerna Series Microscopes
Patch-Clamp Manipulators Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
The Cerna Series is Thorlabs’ platform for electrophysiology, optogenetics, DNA injection, and other applications that require widefield and transmitted light imaging. Just like our Bergamo II Series for multiphoton microscopy, the Cerna Series adopts a modular approach to microscope construction that allows the user to configure the system to exact experimental requirements. The result is a versatile, expandable, accessible design that is fully compatible with Thorlabs-manufactured and user-supplied accessories.
Options at a Glance n Widefield
Imaging • Nikon Epi-Illuminator with 6-Position Turret • Thorlabs Epi-Illuminator • Nikon Trinoculars • High-Power LED or Liquid Light Guide Illumination Source • One or Two Scientific Cameras Dedicated to Transmitted Light or Fluorescence Detection n Transmitted Light Imaging • Nikon Condenser with 1" Motorized Travel • Differential Interference Contrast (DIC) • Dodt Contrast • Brightfield Imaging
270
n Sample
Holders with Motion Control • Manual or Motorized Micromanipulators for Patch Clamping • Fast XY Stage for Multiple Slides, Multiwell Plates, and Custom Inserts • Rigid Stands for Slides, Recording Chambers, and Custom Inserts, with Optional 1" Motorized Travel in X and Y • Low-Profile XY Platform with 2" Motorized Travel in X and Y n Objectives • Single Objective Holder, Dual Objective Changer, or 5-Position Objective Turret • 1" Motorized Travel in Z • Piezo Objective Holder for Fast Z-Stacks
Electrophysiology
Overview: Cerna Series Microscopes
Cerna Series Microscopes
Cerna microscopes consist of several complementary components and accessories that were specifically engineered for those working in electrophysiology.
Patch-Clamp Manipulators
1 Micromanipulators (See Pages 274 - 277) Available in manual or motorized configurations, these micromanipulators use piezoelectric elements to enable nanometer-resolution positioning.
5 Epi-Illumination To illuminate the field of view from above, Cerna systems can be equipped with industry-standard liquid light guides or broad-spectrum LEDs.
2 Rigid Stands (See Pages 296 - 300) Designed to hold slides, recording chambers, and experimental equipment, these stands are outfitted with variable height adjustment, a height retention collar, and a quick-release clamp.
6 Transmitted Light Illumination Our microscopes are compatible with several kinds of condensers from Nikon, which provide basic illumination from underneath the objective. DIC imaging and Dodt contrast are also supported.
3 Motion Control We manufacture motion control stages that provide one- or two-axis travel for our rigid stands, support rail, or our low-profile XY platform. Travel ranges of 1" or 2" are available.
7 Workstations (See Pages 354 - 377) Our vibrationally stabilized optical tables and ScienceDesks are available in several sizes and with a range of shelving options for storage.
4 Scientific Cameras (See Page 308) Up to two cameras can be attached for patch visualization, differential interference contrast (DIC) imaging, or other transmitted light imaging modalities.
Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
4
1
5
7
2
6
3
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Electrophysiology Cerna Series Microscopes Patch-Clamp Manipulators Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
Sample Cerna System: Entry-Level Electrophysiology Rig for Single-Cell Recording This basic Cerna configuration is designed to perform single-cell patch clamp recordings. The recording chamber and micromanipulators are held in place by our rigid stand sample holders, which offer a quickrelease clamp that makes it easy to lock the device in place at the desired position. In order to position the cell within the field of view, the rigid stand has been mounted on motorized stages that provide 1" of fine translation in X and Y.
n Liquid-Light-Guide-Based
Light Source
for Epi-Illumination n LED-Based Transmitted Light Source n Recording Chamber Holder is Motorized for 1" of Fine Travel in X and Y n Nikon Trinoculars and Scientific Camera for Visualization of the Sample
Widefield Viewing Accessories The sample can be viewed directly using the Nikon trinoculars or recorded using the scientific cameras. For sample excitation, this rig is equipped with a single cube epi-illuminator and a DC-stabilized source that is delivered through a liquid light guide. Alternative illumination sources include Thorlabs’ high-power LEDs or industry-standard broadspectrum LEDs.
Since the micromanipulators and recording chamber are mounted on separate rigid stands, this entry-level configuration is best suited for single-cell recording.
272
Configuration C211
Motion Control for Micromanipulators, Sample Holders, and the Microscope The motorized XY stage, objective focusing module, and condenser focusing module can be controlled by our 3-axis knobbed controllers, which offer variable speed control and permit axes to be disabled on an individual basis. The micromanipulators have two controllers: a joystick for coarse control of the Y, Z, and approach axes, and a knob box for controlling the piezos.
Electrophysiology
Sample Cerna System: Premium Electrophysiology Rig with XY Platform This advanced Cerna configuration has several upgrades compared to the basic system shown on the previous page. Rather than mounting each rigid stand directly to the workstation, the rigid stands are secured to an intermediary XY platform that moves the micromanipulators and recording chamber as one unit. This design choice allows the field of view to be freely translated underneath the objective without having to first withdraw the pipettes.
Configuration C202 n Nikon Epi-Illuminator with 6-Position Filter Cube Turret for Selecting Approriate Filter Sets for Specific Fluorophores n XY Platform Moves Specimen and Apparatus in Unison Over 2" Range in X and Y n Scientific Cameras can be Individually Dedicated to Visible and IR Imaging
Large, Motorized Two-Axis Platform
Dual Camera Ports for Dedicated Purposes
Our XY platform provides 2" of motorized travel in X and Y, offers an array of either 1/4"-20 or M6 x 1.0 tapped holes for securing equipment, and enables access to the focal plane from in front of, alongside, or behind the objective. Moreover, the large 24" x 18" (600 mm x 450 mm) work surface is capable of supporting space-consuming experimental apparatuses that need to be moved concurrently with the rest of the setup.
This rig has also been upgraded with a Nikon dual camera port that allows the user to have dedicated cameras for different imaging modalities. Visible light is sent into the front port and NIR light is sent into the rear port, allowing the user to detect visible fluorescence and obtain an NIR DIC image simultaneously.
Cerna Series Microscopes Patch-Clamp Manipulators Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
For pricing details, please see pages 44 - 49 or contact us at ImagingSales@ thorlabs.com.
By mounting the micromanipulators and recording chamber on a common motorized platform, experimental flexibility is improved in comparison to our entry-level configuration.
The image on the computer screen is courtesy of Dr. Lei Zhang and Professor Joshua Singer, University of Maryland.
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Electrophysiology Cerna Series Microscopes
Burleigh® Micromanipulators
Patch-Clamp Manipulators
Burleigh®, a division of Thorlabs, manufactures leading edge micromanipulator assemblies for electrophysiological research. These assemblies provide precise 3D control of pipette manipulation and are available in either manual or motorized options. Each manipulator is composed of three linear translation stages, PCS-6400TR and PCS-6400TL two rotary stages with lockable Micromanipulators Mounted on Our Gibraltar GHB-BX Platform stops, one set of angle brackets, and a headstage mounting plate. The cross roller bearings of this assembly form a stiff and precise single-axis bearing system with high load capacity and minimal friction. The piezoelectric actuators provide a minimum resolution of 60 nm and minimal drift (<l µm/hr).
Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
Mechanical Stop The headstage pivot (shown in the image to the left) on each micromanipulator allows for simple optimization of the pipette angle of approach on either an inverted or upright microscope. The integrated adjustable stop mechanism allows the approach angle to be repeatedly set with a high degree of reliably. When the stop is set, the assembly can be rotated clear of the microscope to exchange pipettes and then quickly reset to the appropriate angle.
Manual Micromanipulators Features n Solid-State
PCS-5300 Micromanipulator
Stability of Piezoelectric Control n Minimal Drift of <l µm/hr n Adjustable Set Stops Allow Easy Pipette Exchange n Manual Coarse Control Range: 25 mm n Fine Control Optional Ranges: 150 or 300 µm (Minimum Resolution: 60 nm)
Burleigh’s PCS-5000 series of micromanipulators offer excellent control of pipette manipulation for electrophysiology research. All models, except the PCS-520N, use piezoelectric (PZT) control of translation stages to provide smooth and precise movement of the pipette head without inducing drift. These micromanipulators provide superior performance compared to other standard control techniques. For applications that do not require piezo translation, we offer the PCS-520N, which is a purely mechanical micromanipulator assembly. 274
Electrophysiology
Burleigh® Micromanipulators
Cerna Series Microscopes
Axis Control Unit
Patch-Clamp Manipulators
The piezoelectric control employed by our micromanipulators provides smooth and predictable movement with no backlash and minimal drift (<1 µm/hr when in a temperature controlled environment). Coarse control is achieved through the use of a mechanical screw and produces a displacement of 25 mm at 32 threads/inch. Fine control uses our piezoelectric flexure mount and provides a displacement of either 150 µm or 300 µm. Each flexure mount is controlled by one knob of the axis control unit. Three turns on the potentiometer corresponds to full scale of the piezo assembly, yielding a resolution that is 0.04% of the total piezo travel range.
Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
Micromanipulator Axis Control Unit SPECIFICATIONS Travel Resolution Speed
PIEZO
MANUAL
150 or 300 µm
25 mm
<60 nm
32 Threads/Inch
Directly Proportional to Rate of Knob Turns
N/A
Drift
<1 µm/hr
Operator Interface Manipulator Dimensions (L × W ×H)
Axis Control Unit
Manual Micrometer
5" × 6.2" × 7.5" (127 mm × 157 mm × 190.5 mm)
Power Supply
Micromanipulator Power Supply
MANUAL PIEZO PIEZO PIEZO ITEM #a TRAVEL X-AXIS Z-AXIS APPROACH AXIS PCS-5200 25 mm 150 µm 150 µm 150 µm
The PCS-5200, PCS-5300, and PCS-5400 assemblies include an axis control unit and regulated, low noise, 60 V output for the axis control unit. Two voltage outputs enable the power supply to regulate two axis control units. This allows the user to run two micromanipulation assemblies from the same power source.
DESCRIPTION Micromanipulator Assembly, 150 µm Travel for X, Z, and Approach
PCS-5300b 25 mm 150 µm 150 µm 300 µm
Micromanipulator Assembly, 150 µm Travel for X & Z, 300 µm Travel for Approach
PCS-5400c 25 mm 300 µm 300 µm 300 µm
Micromanipulator Assembly, 300 µm Travel for X, Z, and Approach
PCS-520N
25 mm
-
-
-
Micromanipulator Assembly, Manual Only, No Power Supply
PRICE $ 7,450.00 $ 7,950.00 $ 8,750.00 $
3,950.00
PCS-5200N 25 mm 150 µm 150 µm 150 µm
Micromanipulator Assembly, 150 µm Piezo Travel in X, Z, and Approach, No Power Supply
$ 6,950.00
PCS-5300Nb 25 mm 150 µm 150 µm 300 µm
Micromanipulator Assembly, 150 µm Piezo Travel in X & Z, 300 µm Piezo Travel Approach, No Power Supply
$ 7,450.00
PCS-5400Nc 25 mm 300 µm 300 µm 300 µm
Micromanipulator Assembly, 300 µm Piezo Travel in X, Z, and Approach, No Power Supply
$ 8,250.00
aFor more details and pricing information, please contact Burleigh@thorlabs.com bRecommended system for working in slices where longer piezoelectric travel on the approach cRecommended system for working in thick slices where maximum flexibility is needed
axis is required
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Electrophysiology Cerna Series Microscopes
Motorized Micromanipulators
Patch-Clamp Manipulators
Features n Solid-State
Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
PCS-6300CR Motorized Micromanipulator Assembly
Burleigh’s PCS-6000 series of micromanipulators offer excellent control of pipette manipulation for electrophysiology research. These micromanipulators use piezoelectric (PZT) and stepper motor control of translation stages to provide smooth and precise movement of the pipette head without inducing drift.
Stability of Piezoelectric Technology n Minimal Drift of <l µm/hr Adjustable Set Stops Allow Easy Pipette Exchange n Stepper Motor Travel Range: 25.4 mm n Fine Control Optional Ranges: 150 or 300 µm (Minimum Resolution: 60 nm)
Cultured Configuration Z
X X
Tilt
Y
Our motorized micromanipulators are available in either a traditional Rotation configuration or a cultured Cultured Configuration Traditional Configuration configuration. For applications such as brain slice work, we offer the traditional configuration. In these applications, the pipette moves to the slice at an approach angle. Hence, control over X, Z, and the Approach Axis translation is preferred. For patching cultured cells, it is advantageous to have control over the three cardinal directions (X, Y, and Z). Thus, in the cultured configuration, X and Y translation position the pipette over the cell of interest and then the Z translation brings the pipette down for patching. Our assemblies are offered in either Left-Handed (LH) or Right-Handed (RH) configurations.
Axis Control Unit
Micromanipulator Axis Control Unit
276
The PZT control employed by our micromanipulators provide much smoother and predictable movement with no backlash and minimal drift (<1 µm/hr in a properly temperature controlled environment). Coarse control is achieved through the use of a stepper motor and produces a displacement of 1" at 1.6 µm minimum step size. These electrically quiet motors eliminate noise and interference to yield excellent precision. Fine control uses our PZT flexure mount and provides a displacement of either 150 µm or 300 µm. Each flexure mount is controlled by one knob of the axis control unit. Three turns on the potentiometer corresponds to full scale of the piezo assembly, yielding a resolution that is 0.04% of the total piezo travel range.
Electrophysiology Cerna Series Microscopes
Motorized Micromanipulators Stepper Motor Joystick Our motorized micromanipulators can use either a joystick or computer interface to control the stepper motor translation. Additionally, the user is able to store up to two independent positions in memory. Push button control on either control system (joystick or computer) recalls the stored position, allowing the user to quickly and easily move between two predetermined positions on the micromanipulator. SPECIFICATIONS
PIEZO
Travel Resolution Speed
Patch-Clamp Manipulators Physiology Stages Motorized Platforms Rigid Stands
Stepper Motor Joystick
Faraday Cage
MANUAL
150 or 300 µm
25 mm
<60 nm
1.6 µm (Min)
Directly Proportional to Rate of Knob Turns
Continuously Variable from 0.5 nm/s to 2 mm/s
Memory Speed
3.5 mm/s
Drift
<1 µm/hr
Computer Interface
EMF Shielding
USB
Manipulator Dimensions (L × W ×H)
7" × 8.5" × 7.5" (178 mm × 216 mm × 190.5 mm)
Control Box
Power Supply
All our motorized micromanipulator assemblies include an axis control unit, joystick, and a regulated, low noise, 60 V output for PZT and stepper motor control. Each control box can support one complete motorized micromanipulator assembly. The control box accepts an input power range of 100 - 240 V at 50/60 Hz.
Traditional Configuration Power Supply MORORIZED PIEZO PIEZO PIEZO ITEM #* TRAVEL X-AXIS Z-AXIS APPROACH AXIS PCS-6200TR 25 mm 150 µm 150 µm 150 µm
DESCRIPTION Motorized, Traditional Micromanipulator Assembly, Right Handed, 150 µm X, Z, and Approach Travel
PRICE $ 8,495.00
PCS-6300TR 25 mm 150 µm 150 µm 300 µm
Motorized, Traditional Micromanipulator Assembly, Right Handed, 150 µm X & Z, 300 µm Approach Travel
$ 8,895.00
PCS-6400TR 25 mm 300 µm 300 µm 300 µm
Motorized, Traditional Micromanipulator Assembly, Right Handed, 300 µm X, Z, and Approach Travel
$ 9,495.00
PCS-6200TL 25 mm 150 µm 150 µm 150 µm
Motorized, Traditional Micromanipulator Assembly, Left Handed, 150 µm X, Z, and Approach Travel
$ 8,495.00
PCS-6300TL 25 mm 150 µm 150 µm 300 µm
Motorized, Traditional Micromanipulator Assembly, Left Handed, 150 µm X & Z, 300 µm Approach Travel
$ 8,895.00
PCS-6400TL 25 mm 300 µm 300 µm 300 µm
Motorized, Traditional Micromanipulator Assembly, Left Handed, 300 µm X, Z, and Approach Travel
$ 9,495.00
*For more details and pricing information, please contact Burleigh@thorlabs.com
Cultured Configuration MORORIZED PIEZO PIEZO PIEZO ITEM #* TRAVEL X-AXIS Z-AXIS Y-AXIS PCS-6200CR 25 mm 150 µm 150 µm 150 µm PCS-6300CR 25 mm 150 µm 150 µm 300 µm
DESCRIPTION Motorized, Cultured Micromanipulator Assembly, Right Handed, 150 µm X, Y, and Z Travel Motorized, Cultured Micromanipulator Assembly, Right Handed, 150 µm X and Z Travel, 300 µm Y Travel
PRICE $ 8,495.00 $ 8,895.00
PCS-6400CR 25 mm 300 µm 300 µm 300 µm
Motorized, Cultured Micromanipulator Assembly, Right Handed, 300 µm X, Y, and Z Travel
$ 9,495.00
PCS-6200CL 25 mm 150 µm 150 µm 150 µm
Motorized, Cultured Micromanipulator Assembly, Left Handed, 150 µm X, Y, and Z Travel
$ 8,495.00
PCS-6300CL 25 mm 150 µm 150 µm 300 µm
Motorized, Cultured Micromanipulator Assembly, Left Handed, 150 µm X and Z Travel, 300 µm Y Travel
$ 8,895.00
PCS-6400CL 25 mm 300 µm 300 µm 300 µm
Motorized, Cultured Micromanipulator Assembly, Left Handed, 300 µm X, Y, and Z Travel
$ 9,495.00
*For more details and pricing information, please contact Burleigh@thorlabs.com
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Electrophysiology Cerna Series Microscopes Patch-Clamp Manipulators Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
Headstage Adapter Features n Allows
Orientation of the Headstage and Pipette Holder at Very Steep or Very Shallow Angles n Sliding Stage with Repeatable Stops at Both Ends n Quick and Easy Pipette Exchange n Left- and Right-Handed Compatible The PCS-500-SSH Steep/Shallow Angle Headstage Adapter is designed to provide a flexible platform for mounting pipettes at angles >45° and <25°. The mounting bracket features a manual slide with 3" of translation and stops at both ends for repeatable positioning. Mounting holes are provided for the AXON CV-5, AXON CV-203B, and HEKA EPC-9 commercial headstages. The adapter consists of the headstage adapter plate, the slide assembly with a dovetail on the back, a clamping plate, and a mounting plate. After installing the mounting plate on the micromanipulator at the desired angle using the included 4-40 screws, the slide is clamped in one of the dovetail grooves on the mounting plate using the clamping plate and included 2-56 screws. The location of the end stops can be easily adjusted by turning PCS-500-SSH Headstage Adapter Mounted on a the locking handle to Micromanipulator loosen the clamping Assembly (See Pages 274 - 277) plate, sliding the slide assembly to the desired position, and retightening the clamp.
PCS-500-SSH
Once the headstage adapter is installed, the pipette holder can be moved along the slide by loosening the locking handle. To exchange pipettes, slide the headstage adapter back so that it is clear of your sample and the microscope. Rotate the base pivot on your micromanipulator to access your pipette. Once the new pipette is installed, repeat these two steps in reverse to return the pipette to its original location. Steep pipette angles are often desired in electrophysiology experiments in order to minimize the length of the electrode in solution. The shorter the length of the pipette in solution, the lower the stray capacitance and electrical noise measured during recording. In these circumstances, the pipette angle is typically greater than 45°. The position of other microscope components, such as the condenser lens of an inverted microscope, can interfere with the headstage, thereby limiting the angle of maximum approach. The use of a headstage adapter on the top of the manipulator creates a lower profile, providing extra clearance that allows the steepness of the pipette to be maximized. For slice physiology, the micromanipulator should be positioned so that the tip of the electrode moves in a straight line over the surface of the brain slice chamber. A shallow angle, typically 25° to 15°, is required to avoid interfering with the microscope objective and breaking the pipette. This shallow angle is also helpful when mounting multiple pipettes around the sample.
ITEM # PCS-500-SSH
278
PRICE $ 750.00
DESCRIPTION Steep/Shallow Angle Headstage Adapter for Micromanipulator Assembly
Electrophysiology Cerna Series Microscopes
Pipette Holder Mount
Patch-Clamp Manipulators
Features n Allows
Orientation of the Pipette Holder at Very Steep Angles n Left- and Right-Handed Compatible The MIS-PHM Pipette Holder Mount accepts pipette holders up to 4" in diameter. It features a moveable rail that allows the pipette to be easily positioned in a variety of locations. The rotational freedom of the pipette holder clamp assembly allows you to achieve both steep and shallow angles, depending on your needs.
Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
MIS-PHM Shown Mounted on a Micromanipulator
ITEM # MIS-PHM
PRICE $ 771.00
DESCRIPTION Pipette Holder Mount for Micromanipulator Assembly
Have you seen our...
XYZ Microscope Stages ◆ Compatible with Nikon, Olympus, and Zeiss Microscopes ◆ XY DC Linear Servo Stage with Position Accuracy of <3.0 µm ◆ Z-Axis Piezo Stage with 500 µm of Vertical Travel and
25 nm Resolution ◆ Options for 2-Axis or Z-Axis Joystick Control
Thorlabs’ motorized XYZ microscope stage system presents a 3D positioning solution for applications such as Z-axis slicing or 3D image collection, including laser scanning microscopy. It provides active feedback, with submicron repeatability and 25 nm resolution, to compensate for thermal changes and other factors that might lead to stage drift. A rich selection of accessories makes this stage adaptable to a wide variety of research applications.
MZF001 MZS500-E
See Pages 174 - 177 279
Electrophysiology Cerna Series Microscopes Patch-Clamp Manipulators
Electrophysiology Platform Overview Gibraltar Series Platforms
Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
n Ultra-Stable
Platform n Ideal for Multi-Site Electrophysiology n Large Stage Provides Space for Manipulators and Other Instrumentation n Manual and Motorized Versions Available n Stage Hole Pattern on 1" Centers n Stage Supported by 4 Adjustable-Height Pillars n Compatible with Olympus, Nikon, Zeiss, and Leica
PHYS24 Shown with Nikon FN1 Microscope
Thorlabs offers two lines of Electrophysiology Stages: the Gibraltar series (shown above) from Thorlabs’ acquisition of the Burleigh® Electrophysiology product line and the PHYS24 series (shown right) developed internally. Both are now manufactured and supported by Thorlabs. There are many similarities between the two product lines and a few differences, which are summarized below.
Top Plate
Manual Stage
280
GIBRALTAR SERIES (SEE PAGES 281 – 286)
PHYS24 SERIES (SEE PAGES 287 – 289)
Cast Aluminum Stage with 1/4" Through Holes on 1" Centers Magnetic Stainless Steel Honeycomb Stage with 1/4"-20 Taps on 1" Centers
Aluminum Honeycomb Stage with 1/4"-20 (M6) Taps on 1" (25 mm) Centers
XY Translation Range: 1" Micrometer Graduation: 25 µm Mechanism for Locking Stage Position
XY Translation Range: 2" (50 mm) Micrometer Vernier Graduation: 10 µm No Lock for Stage Position
Motorized Stage
XY Translation Range: 1" Resolution: <5 µm (at Min Speed of 3.0 µm/s)
Motorized Stage Control
Joystick with Buttons for Two Preset Locations
Supported Microscopes
Olympus BX70, BX71, BX50W1, & BX51WI Zeiss Axioskop FS, Axioskop 2FS, & Axio Examiner Nikon FN1 & E600FN Leica DM LFS
Unencoded Stage XY Translation Range: 2" (50 mm) On-Axis Accuracy: 10 µm Max Speed: 1 mm/s Encoded Stage XY Translation Range: 2" (50 mm) On-Axis Accuracy: 3 µm Max Speed: 4 mm/s Thorlabs apt™ Software Package (Includes a Graphical User Interface and SDK) Nikon FN1
Electrophysiology Cerna Series Microscopes
Gibraltar® Platforms for Electrophysiology n Compatible
with Olympus, Zeiss, Nikon, and Leica Microscopes to Integrate Micromanipulators n Solid Aluminum or Stainless Steel Breadboard n Manual Micrometer or Stepper Motor Control n Designed
Patch-Clamp Manipulators Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
Burleigh®, a division of Thorlabs, manufactures leading edge equipment for electrophysiology research such as the Gibraltar® Platforms. These platforms are a stable and flexible mechanical solution for electrophysiology research. The XY platform provides reliable and reproducible movement, either manual or motorized, of the microscope relative to the Gibraltar stage. This allows the user to change the field of view (FOV) without moving the sample itself, thus preventing disruption to patch recording.
Applications n Multiple-Patch
Experiments n Time-Lapse Photography n Photolysis and Patch Clamping n Recording in Different Fields of View n Whole Specimen Imaging
Gibraltar platforms can support the installation of multiple micromanipulators, chambers, or other instruments around the microscope objective while providing superior mechanical and thermal stability. This stability is particularly important in sensitive electrophysiology research such as multiple patching. The platform has four large-diameter columns that rigidly support the platform, enabling it to be directly bolted to an optical table or mounted to our baseplate. When combined with our micromanipulators, the Gibraltar platform gives unparalleled control over pipette and microscope position.
Baseplate Our Gibraltar Platform is available with or without an attachable baseplate that has rubber grommet feet to prevent accidental movement and provide vibrational isolation. Both options are a secure, stable, and static mounting solution for our platform. The baseplate allows position flexibility, giving the user the option to move the platform without deconstructing it first. When purchased without a baseplate, the Gibraltar platform is mounted directly to any imperial or metric optical table or to a ScienceDesk™ (see pages 354 - 367).
Baseplate with Rubber Feet
281
Electrophysiology Cerna Series Microscopes Patch-Clamp Manipulators Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
Gibraltar® Platforms for Electrophysiology Top Plate Options Our Gibraltar Electrophysiology platforms are available with either a solid aluminum or stainless steel top plate. The 1/2" solid aluminum top plate delivers a stable platform for mounting hardware and equipment near the microscope objective. With through holes for 1/4"-20 (M6) bolts, this plate can be integrated with either imperial or metric devices. Our stainless steel top plate offers several advantages over the solid aluminum plate. The low coefficient of expansion for stainless steel provides excellent thermal stability, while the honeycomb structure furnishes vibrational damping. This honeycomb top plate has 1/4"-20 tapped holes, allowing hardware to be directly mounted to the surface with hex screws. Each tapped hole is sealed with a nylon-based cup, so liquid spills on the surface will not drip down onto the microscope. Additionally, the plate is magnetic, allowing the user to place hardware in the optimal position through the use of a magnetic base. The honeycomb structure provides excellent thermal stability and vibrational damping.
Aluminum Top Plate
Stainless Steel Top Plate
Translation Options The Gibraltar’s XY translation stage for microscope position manipulation is available with either manual or motorized translators.
Manual Translation Stage
Manual Translation Manual translation is accomplished through two simple, yet precise, mechanical micrometers. These micrometers provide 1" (25.4 mm) of translation in both directions with a resolution of less than 5 µm. Additionally, these micrometers feature a friction lock, allowing the user the ability to lock down the translation stage, preventing movement of the microscope relative to the sample.
Motorized Translation The motorized option has two fast and precise stepper motors that provide 1" of translation in both directions with a resolution of 5 µm. The motorized translation stage offers several advantages over the manual version. These motors utilize a simple joystick for user interface that can translate the stage in both the X and Y directions. Two speeds (0.4 mm/s and 3.0 µm/s) are available for user convenience. The joystick gives the user the ability to save two independent positions to memory. The saved positions can be recalled at the touch of a button, ideal for experiments that demand quick and accurate investigation of two different fields of Motorized Translation Stage view within the same sample. 282
Electrophysiology
Gibraltar® Platforms for Electrophysiology
Cerna Series Microscopes
Thorlabs offers four versions of our motorized and four versions of our manual Gibraltar platform for use with select microscopes from Olympus, Zeiss, Nikon, and Leica. Our platform can support the installation of multiple micromanipulators, chambers, or other instruments around the microscope objective while providing superior mechanical and thermal stability. A hardware kit for assembling the Gibraltar platform is included.
Patch-Clamp Manipulators
The Gibraltar platform’s flexibility and solid design provides an ideal solution for any electrophysiology system. Its heavy-duty frame can support numerous experimental apparatuses up to 25 lbs (11.34 kg) of weight, damps vibrations, and is compact enough to be incorporated onto an optical table. Its modularity and adjustability allows this platform to accommodate various microscopes, hardware, and purpose.
GIBRALTAR PLATFORM TOP (CHOOSE ONE) Material
Black Epoxy-Coated Aluminum
Magnetic Stainless Steel
Dimensions
23.6" × 17" × 0.5"
23.6" × 17" × 1.25"
Hole Pattern
1/4" Holes on 1" Centers
1/4"-20 Holes on 1" Centers
TRANSLATION MECHANISM (CHOOSE ONE) Type
Motorized
Manual
Range
1" (25.4 mm) in Both X and Y
1" (25.4 mm) in Both X and Y
Speed
3.0 µm/s to 0.4 mm/s
N/A
5 µm at Min Speed
<5 µm
Resolution
Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
BASEPLATE (OPTIONAL) Material Dimensions
Black Epoxy-Coated Aluminum 23.6" × 11.6" (599 mm × 295 mm)
SAMPLE PLATE Material
Black-Plated Aluminum with Rubber Feet
Dimensions
110 mm OD, 32 mm ID, 3.2 mm Thick
Gibraltar Platforms for Olympus BX70, BX71, BX50WI, and BX51WI Upright Microscopes ITEM #
PRODUCT IMAGE
DESCRIPTION
PRICE
G-BX
Manual Gibraltar Stage for Olympus Microscopes, Aluminum Platform w/o Baseplate
$ 6,000.00
GH-BX
Manual Gibraltar Stage for Olympus Microscopes, Stainless Steel Platform w/o Baseplate
$9,000.00
GB-BX
Manual Gibraltar Stage for Olympus Microscopes, Aluminum Platform with Baseplate
$6,500.00
GHB-BX
Manual Gibraltar Stage for Olympus Microscopes, Stainless Steel Platform with Baseplate
$9,500.00
GM-BX
Motorized Gibraltar Stage for Olympus Microscopes, Aluminum Platform w/o Baseplate
$7,500.00
GMH-BX
Motorized Gibraltar Stage for Olympus Microscopes, Stainless Steel Platform w/o Baseplate
$10,500.00
GMB-BX
Motorized Gibraltar Stage for Olympus Microscopes, Aluminum Platform with Baseplate
$8,000.00
GMHB-BX
Motorized Gibraltar Stage for Olympus Microscopes, Stainless Steel Platform with Baseplate
$11,000.00
283
Electrophysiology Cerna Series Microscopes Patch-Clamp Manipulators Physiology Stages
GibraltarÂŽ Platforms for Electrophysiology Gibraltar Platforms for Zeiss Axioskop FS Upright Microscopes ITEM #
PRODUCT IMAGE
DESCRIPTION
PRICE
G-FS
Manual Gibraltar Stage for Zeiss Axioskop FS Microscopes, Aluminum Platform w/o Baseplate
$6,000.00
GH-FS
Manual Gibraltar Stage for Zeiss Axioskop FS Microscopes, Stainless Steel Platform w/o Baseplate
$9,000.00
GB-FS
Manual Gibraltar Stage for Zeiss Axioskop FS Microscopes, Aluminum Platform with Baseplate
$6,500.00
GHB-FS
Manual Gibraltar Stage for Zeiss Axioskop FS Microscopes, Stainless Steel Platform with Baseplate
$9,500.00
GM-FS
Motorized Gibraltar Stage for Zeiss Axioskop FS Microscopes, Aluminum Platform w/o Baseplate
$7,500.00
GMH-FS
Motorized Gibraltar Stage for Zeiss Axioskop FS Microscopes, Stainless Steel Platform w/o Baseplate
$10,500.00
GMB-FS
Motorized Gibraltar Stage for Zeiss Axioskop FS Microscopes, Aluminum Platform with Baseplate
$8,000.00
GMHB-FS
Motorized Gibraltar Stage for Zeiss Axioskop FS Microscopes, Stainless Steel Platform with Baseplate
$11,000.00
Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
Gibraltar Platforms for Zeiss Axioskop 2FS Microscopes ITEM #
284
PRODUCT IMAGE
DESCRIPTION
PRICE
G-2FS
Manual Gibraltar Stage for Zeiss Axios 2FS Microscopes, Aluminum Platform w/o Baseplate
$6,000.00
GH-2FS
Manual Gibraltar Stage for Zeiss Axios 2FS Microscopes, Stainless Steel Platform w/o Baseplate
$9,000.00
GB-2FS
Manual Gibraltar Stage for Zeiss Axios 2FS Microscopes, Aluminum Platform with Baseplate
$6,500.00
GHB-2FS
Manual Gibraltar Stage for Zeiss Axios 2FS Microscopes, Stainless Steel Platform with Baseplate
$9,500.00
GM-2FS
Motorized Gibraltar Stage for Zeiss Axios 2FS Microscopes, Aluminum Platform w/o Baseplate
$7,500.00
GMH-2FS
Motorized Gibraltar Stage for Zeiss Axios 2FS Microscopes, Stainless Steel Platform w/o Baseplate
$10,500.00
GMB-2FS
Motorized Gibraltar Stage for Zeiss Axios 2FS Microscopes, Aluminum Platform with Baseplate
$8,000.00
GMHB-2FS
Motorized Gibraltar Stage for Zeiss Axios 2FS Microscopes, Stainless Steel Platform with Baseplate
$11,000.00
Electrophysiology Cerna Series Microscopes
GibraltarÂŽ Platforms for Electrophysiology Gibraltar Platforms for Zeiss Axio Examiner Microscopes ITEM #
PRODUCT IMAGE
G-AE
DESCRIPTION
PRICE
Manual Gibraltar Stage for Zeiss Axio Examiner Microscopes, Aluminum Platform w/o Baseplate
$6,000.00
Physiology Stages Motorized Platforms
Manual Gibraltar Stage for Zeiss Axio Examiner Microscopes, Stainless Steel Platform w/o Baseplate
$9,000.00
GB-AE
Manual Gibraltar Stage for Zeiss Axio Examiner Microscopes, Aluminum Platform with Baseplate
$6,500.00
GHB-AE
Manual Gibraltar Stage for Zeiss Axio Examiner Microscopes, Stainless Steel Platform with Baseplate
$9,500.00
GM-AE
Motorized Gibraltar Stage for Zeiss Axio Examiner Microscopes, Aluminum Platform w/o Baseplate
$7,500.00
GMH-AE
Motorized Gibraltar Stage for Zeiss Axio Examiner Microscopes, Stainless Steel Platform w/o Baseplate
$10,500.00
GMB-AE
Motorized Gibraltar Stage for Zeiss Axio Examiner Microscopes, Aluminum Platform with Baseplate
$8,000.00
GMHB-AE
Motorized Gibraltar Stage for Zeiss Axio Examiner Microscopes, Stainless Steel Platform with Baseplate
$11,000.00
GH-AE
Patch-Clamp Manipulators
Rigid Stands Faraday Cage EMF Shielding
Gibraltar Platforms for Nikon FN1 Upright Microscopes ITEM #
PRODUCT IMAGE
DESCRIPTION
PRICE
G-FN1
Manual Gibraltar Stage for Nikon FN1 Microscopes, Aluminum Platform w/o Baseplate
$6,000.00
GH-FN1
Manual Gibraltar Stage for Nikon FN1 Microscopes, Stainless Steel Platform w/o Baseplate
$9,000.00
GB-FN1
Manual Gibraltar Stage for Nikon FN1 Microscopes, Aluminum Platform with Baseplate
$6,500.00
GHB-FN1
Manual Gibraltar Stage for Nikon FN1 Microscopes, Stainless Steel Platform with Baseplate
$9,500.00
GM-FN1
Motorized Gibraltar Stage for Nikon FN1 Microscopes, Aluminum Platform w/o Baseplate
$7,500.00
GMH-FN1
Motorized Gibraltar Stage for Nikon FN1 Microscopes, Stainless Steel Platform w/o Baseplate
$10,500.00
GMB-FN1
Motorized Gibraltar Stage for Nikon FN1 Microscopes, Aluminum Platform with Baseplate
$8,000.00
GMHB-FN1
Motorized Gibraltar Stage for Nikon FN1 Microscopes, Stainless Steel Platform with Baseplate
$11,000.00
285
Electrophysiology Cerna Series Microscopes Patch-Clamp Manipulators Physiology Stages
GibraltarÂŽ Platforms for Electrophysiology Gibraltar Platforms for Nikon E600FN Upright Microscopes ITEM #
PRODUCT IMAGE
DESCRIPTION
PRICE
G-EFN
Manual Gibraltar Stage for Nikon E600FN Microscopes, Aluminum Platform w/o Baseplate
$6,000.00
GH-EFN
Manual Gibraltar Stage for Nikon E600FN Microscopes, Stainless Steel Platform w/o Baseplate
$9,000.00
GB-EFN
Manual Gibraltar Stage for Nikon E600FN Microscopes, Aluminum Platform with Baseplate
$6,500.00
GHB-EFN
Manual Gibraltar Stage for Nikon E600FN Microscopes, Stainless Steel Platform with Baseplate
$9,500.00
GM-EFN
Motorized Gibraltar Stage for Nikon E600FN Microscopes, Aluminum Platform w/o Baseplate
$7,500.00
GMH-EFN
Motorized Gibraltar Stage for Nikon E600FN Microscopes, Stainless Steel Platform w/o Baseplate
$10,500.00
GMB-EFN
Motorized Gibraltar Stage for Nikon E600FN Microscopes, Aluminum Platform with Baseplate
$8,000.00
GMHB-EFN
Motorized Gibraltar Stage for Nikon E600FN Microscopes, Stainless Steel Platform with Baseplate
$11,000.00
Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
Gibraltar Platforms for Leica DM LFS Upright Microscopes ITEM #
286
PRODUCT IMAGE
DESCRIPTION
PRICE
G-LFS
Manual Gibraltar Stage for Leica DM LFS Microscopes, Aluminum Platform w/o Baseplate
$6,000.00
GH-LFS
Manual Gibraltar Stage for Leica DM LFS Microscopes, Stainless Steel Platform w/o Baseplate
$9,000.00
GB-LFS
Manual Gibraltar Stage for Leica DM LFS Microscopes, Aluminum Platform with Baseplate
$6,500.00
GHB-LFS
Manual Gibraltar Stage for Leica DM LFS Microscopes, Stainless Steel Platform with Baseplate
$9,500.00
GM-LFS
Motorized Gibraltar Stage for Leica DM LFS Microscopes, Aluminum Platform w/o Baseplate
$7,500.00
GMH-LFS
Motorized Gibraltar Stage for Leica DM LFS Microscopes, Stainless Steel Platform w/o Baseplate
$10,500.00
GMB-LFS
Motorized Gibraltar Stage for Leica DM LFS Microscopes, Aluminum Platform with Baseplate
$8,000.00
GMHB-LFS
Motorized Gibraltar Stage for Leica DM LFS Microscopes, Stainless Steel Platform with Baseplate
$11,000.00
Electrophysiology
Physiology Stages
Cerna Series Microscopes
Features n Adjustable-Height
PHYS24
U-Shaped Breadboard Provides 270° Access to Samples n Includes Microscope Translator (Three Versions Available) • Manual Translation • Motorized Translation • Encoded Motorized Translation n U-Shaped UltraLight™ Series Breadboard with Sealed Holes to Contain Spills n Imperial and Metric Versions n Ships Fully Assembled
In addition to the Gibraltar Electrophysiology Platforms presented on pages 281 - 286, Thorlabs also offers an alternative physiology stage option for use with the Nikon Eclipse FN1 upright microscope, our PHYS24 Series. This option provides twice the XY translation range (2" compared to 1" on the Gibraltar version) and incorporates an aluminum honeycomb top plate instead of a magnetic stainless steel one. Other differences between the physiology stage featured here and the Gibraltar option are outlined on page 280.
Patch-Clamp Manipulators Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
Like the Gibraltar stages, Thorlabs’ PHYS24 series of physiology stages provide a rigid structure for electrophysiological research while supporting the precise motion necessary for the study of physiological processes in living cells and animals. A manual or motorized platform enables the microscope to be moved relative to the position of the specimen while keeping the specimen stationary during image acquisition. The physiology stages featured here consist of a rigid, mechanically and thermally stable U-shaped breadboard that provides more than two hundred 1/4"-20 (M6) tapped holes over a full 270°, which makes it easy to mount patch clamps or other experimental accessories. The upper U-shaped breadboard is supported by four adjustable-height support columns. The base of each column is attached to a 1/2" (12.7 mm) thick solid aluminum breadboard with 1/4"-20 (M6) taps. Each physiology stage comes with either a manual or motorized 2" (50 mm) XY Travel Microscope Translator that is mounted to the base breadboard and provides smooth, repeatable movement in the XY direction while maintaining a stable platform for the microscope.
Baseplate The PHYS24 Series of Physiology Stages include a 24" × 24" (600 mm × 600 mm), 1/2" (12.7 mm) thick solid aluminum breadboard. This black, low-reflective, anodized baseplate features 1/4"-20 (M6) tapped holes on 1" (25 mm) centers. The baseplate offers position flexibility, allowing the user to move the platform without deconstructing it first. Four 1/4" (M6) counterbored clearance holes allow the platform to be secured to an optical table to prevent accidental movement.
Solid Aluminum Breadboard Baseplate
Aluminum Top Plate
Aluminum Top Plate Shown with Insert Removed
Our UltraLightTM honeycomb top plate features 1/4"-20 (M6) tapped holes, allowing hardware to be directly mounted to the surface with hex screws. Each tapped hole is sealed with a nylon-based cup, so liquid spills on the surface will not drip down onto the microscope. The honeycomb structure provides excellent vibrational damping.
287
Electrophysiology Cerna Series Microscopes Patch-Clamp Manipulators Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
Physiology Stages PHYS12P
Support Posts The PHYS24 series of stages include four stainless steel support posts, offering 8" to 12" of continuous height adjustment. Each post has a top-located 1/4"-20 (M6) tap, a universal base with four 1/4" (M6) counterbored holes on 2" (50 mm) centers for mounting it to an optical table or breadboard, and can support a load capacity of 300 lbs (136 kg). Down
Half Up
Up
Translation Options Thorlabs’ PHYS24 Series of Physiology Stages offers state-of-the-art workstations that are available with manual, motorized, or motorized-encoded XY translation stages. These stages are directly compatible with the Nikon Eclipse FN1 upright microscope. Please email ImagingSales@thorlabs.com to inquire about adapter plates that are compatible with other common microscopes.
MT-FN1 Manual Microscope Translator
Manual Translation Stage Manual translation is accomplished through two simple, yet precise, mechanical micrometers. These micrometers provide 2" (50 mm) of translation in both directions with 10 µm vernier graduations. Smooth and repeatable positioning ensures reliable translation of the microscope across a large area of the sample. This stage is easily configured in either a left- or right-handed XY Orientation. See pages 290 - 291 for more details.
Motorized Translation Stage The motorized translation stages utilize high-precision stepper motors to achieve translation. These motors provide 2" (50 mm) of translation in both directions. They feature a repeatability of 0.5 μm with an absolute positioning accuracy of 10 μm over the full travel range. USB plug-and-play operation ensures a simple and quick computer setup. Our motorized translators come with two TST101 T-Cube Stepper Motors for automated control. See pages 290 - 291 for more details.
MTM-FN1 Motorized Microscope Translator
Motorized with Encoder Translation Stage
BSC202 Two-Channel Stepper Motor Driver
MTME-FN1 Motorized Microscope Translator with Encoder
288
The motorized stage with built-in encoder offers several advantages over the motorized stage. The linear optical encoder mounts to the moving platform of the stage and provides feedback to the drive electronics to ensure a bi-directional repeatability. A repeatability of 0.3 μm is achievable with encoders (versus 0.5 μm without) with an absolute positioning accuracy of 3 μm over the full travel rang. Our motorized with encoder stages come with our BSC202 Two-Channel apt™ Benchtop Stepper Motor. See pages 290 - 291 for more details.
4.7" (119 mm) Ø4.3" (Ø110 mm)
24.0" (610 mm)
Physiology Stages
Cerna Series Microscopes
Microscope Translator Specifications 13.4" (340 mm) Manual*
Patch-Clamp Manipulators
23.6" (600 mm)
Ø4.3" (Ø110 mm)
10.0" 8.5" - 12"] 254 mm)
Electrophysiology
n XY
8.7" (221 mm)
11.5" (292 mm)
10.0" [8.5" - 12"] (254 mm)
24.0" (610 mm)
Motorized-Encoded*
17.7" (450 mm) Travel: 2"
(50 mm) n 15.5" Adjustment: Micrometer (394 mm) n Vernier Graduations: 10 4.2" µm (106 mm) n Load 2.8"Capacity: 110 lbs (50 kg) (70 mm)
Motorized*
17.7" (450 mm)
n XY
15.5" (394 mm) 4.2" (106 mm) 2.8" (70 mm) 24.0" (610 mm)
7.1" (180 mm)
4.7" (119 mm)
Travel: 2" (50 mm) n Adjustment: Stepper Motor n Velocity (Max): 1 mm/s n Acceleration (Max): 0.5 mm/s2 n On-Axis Accuracy: 10 µm n Bi-Directional Repeatability: 0.5 µm n Load Capacity: 110 lbs (50 kg)
n XY
Travel: 2" (50 mm) n Adjustment: Stepper Motor n Velocity (Max): 4 mm/s n Acceleration (Max): 3 mm/s2 n On-Axis Accuracy: 3 µm Over the Full Travel n Bi-Directional Repeatability: 0.3 µm n Velocity Stability: ±0.4 mm/s n Encoder: Optical Grating Incremental Encoder n Encoder Resolution: 0.1 µm n Load Capacity: 110 lbs (50 kg)
Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
*Components Available Separately on the Following Pages
Ø4.3" (Ø110 mm)
24.0" (610 mm)
PHYS24ME Motorized-Encoded Physiology Stages (Nikon FN1 Microscope Not Included)
13.4" (340 mm) 17.7" (450 mm)
23.6" (600 mm) 11.5" (292 mm)
8.7"
10.0" [8.5" - 12"] (254 mm) Two Controllers Included with the PHYS24M(/M) Motorized Physiology 24.0"Stage (610 mm)
TST101 (221 mm)
15.5" (394 mm) 4.2" (106 mm) 2.8" (70 mm)
BSC202 Two-Channel Stepper Motor Controller Included with the PHYS24ME(/M) Physiology Stage with Encoders ITEM # PHYS24
$
PRICE 7,500.00
DESCRIPTION Manual Physiology Stage (Imperial)
PHYS24/M
$
7,500.00
Manual Physiology Stage (Metric)
PHYS24M
$
10,000.00
Motorized Physiology Stage (Imperial)
PHYS24M/M
$
10,000.00
Motorized Physiology Stage (Metric)
PHYS24ME
$
14,500.00
Motorized Physiology Stage with Encoders (Imperial)
PHYS24ME/M
$
14,500.00
Motorized Physiology Stage with Encoders (Metric)
289
Electrophysiology Cerna Series Microscopes
Microscope Translators
Patch-Clamp Manipulators
Thorlabs offers precision Microscope Translators to position n Manual, Motorized, and Motorizeda microscope about a stationary specimen. This method of Encoded Versions Available positioning the field of view of the microscope enables viewing n 2" (50 mm) XY Travel Range of a large area of a sample without moving the specimen. n 110 lbs (50 kg) Load Capacity The Microscope Translators provide travel of the microscope (XY Travel Platform) in both the X and Y directions while maintaining a rigid, n Rugged, Thermally Stable Aluminum mechanically and thermally stable platform for the microscope. Mounting and Spacer Plates These translators are constructed of solid stainless steel and n Heavy-Duty Cross-Roller consist of four LNR50 series stages (one master and one slave Stainless-Steel Bearings for each axis) mounted on a baseplate and connected by a top plate for mounting the microscope. The array of counterbored holes in the baseplate allows the translator to be secured to an imperial or metric tapped breadboard or optical table. Thorlabs’ standard microscope translators have a top plate that is compatible with the Nikon Eclipse FN1 upright microscope. Please email ImagingSales@thorlabs.com to inquire about top plates that are compatible with other common upright microscopes.
Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
Manual Microscope Translator MT-FN1 Specifications n 2"
(50 mm) XY Travel Range n 50 µm/rev n 10 µm/Graduation
Features
The MT-FN1 Manual Microscope Translator can be easily configured in either a left- or right-handed XY orientation. It utilizes micrometer drives with a graduated scale to provide smooth translation and repeatable positioning, which enables the microscopist to return to a previously viewed location. MT-FN1
Motorized Microscope Translator The motorized version of the Microscope Translator (MTM-FN1) includes high-quality stepper-motor-based actuators (DRV014) to provide automated high-resolution XY positioning and repeatability. Each stepper-motor-based actuator utilizes a trapezoidal-shaped 1 mm/rev pitch lead screw that provides high load carrying capability. Coupled with stepper motor controllers (two TST101 controllers included), the Motorized Microscope Translator provides smooth automated-controlled movement in increments of 0.05 µm/step.
Features n Single-Channel
Controller n High-Resolution Microstepping n Compact Footprint n USB Plug-and-Play PC-Controlled Operation n Easy-to-Use Manual Controls with Velocity Slider and Jog Buttons n Full Software Control Suite Supplied n Extensive ActiveX® Programming Interfaces n Software Integrated with Other apt™ Family Controllers 290
ITEM #
MTM-FN1
Travel Range
2" (50 mm)
Acceleration
0.5 mm/s2 (Max)
Velocity
1.0 mm/s (Max)
Maximum Load Capacity Incremental Movement
110 lbs (50 kg) 0.05 µm (Min Achievable)
Bidirectional Repeatability
0.5 µm
Percent Positional Accuracy
0.02% (Max)
Absolute Accuracy Home Location Accuracy Drive Runout (Over Full Range) Lead Screw Pitch Limit Switches Included Controllers* Controller Interface Bearings
10 µm ±1.0 µm DRV014 ±0.0004" (±10 µm) 1 mm Ceramic-Tipped ElectroMechanical Switches TST101 USB Crossed Roller
* Two TST101 controllers included. Please visit www.thorlabs.com for details and specifications.
TST101 One-Channel Model
MTM-FN1
Electrophysiology
Microscope Translators
Cerna Series Microscopes
Motorized Microscope Translator with Encoder
Patch-Clamp Manipulators
The performance of the Motorized Microscope Translator is further enhanced by utilizing XY stages with high-resolution integrated linear optical encoders in combination with Thorlabs’ two-channel closed-loop stepper motor controller (BSC202). The linear optical encoder provides feedback to the drive electronics to ensure accurate positioning and allows a direct readout of the absolute MTME-FN1 position of the stage. With a resolution of 0.1 µm, the bi-directional position accuracy is greater than 0.3 µm (compared to 0.5 µm without the encoders) over the full 50 mm of travel. The motorized microscope translator with optical encoders allows the user to return to a previous position within the specimen and is the ideal solution for applications where stability, long microscope travel, and high-load Specifications capacity need to be achieved ITEM # MTME-FN1 with absolute position accuracy. Travel Range 2" (50 mm) Acceleration
3.0 mm/s2 (Max)
Velocity
4.0 mm/s (Max)
Maximum Load Capacity
110 lbs (50 kg)
Incremental Movement
0.05 µm (Min Achievable)
Bidirectional Repeatability
0.3 µm
Percent Accuracy
0.02% (Max)
Absolute Accuracy
3 µm Over the Full Travel
Home Location Accuracy
±1.0 µm
Drive
DRV014
Runout (Over Full Range)
Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
Thorlabs’ PHYS24ME Physiology Stage includes the MTME-FN1 Microscope Translator, as shown here with a Nikon FN1 Microscope (Not Included)
±0.0004" (±10 µm)
Lead Screw Pitch Limit Switches
Physiology Stages
1 mm Ceramic-Tipped Electro-Mechanical Switches
Included Controllers*
BSC202
Controller Interface
USB
Encoder
Optical Grating Incremental Encoder
Encoder Resolution
0.1 µm
Bearings
Crossed Roller
*Specifications for the BSC202 Controller can be found online at www.thorlabs.com.
aptTM Software Screenshot
BSC202
Controller Features n Two-Channel
Models Available n Supports 2-Phase Bipolar Steppers up to 50 W n Differential Encoder Feedback (QEP) Inputs for Closed-Loop Positioning n USB Plug-and-Play Plus Multi-Axis Expansion n Motor Control I/O Port (Jogging, Interlocks) n Full Software GUI Control Suite n High-Resolution Microstepping Control (For Very Fine Positioning Applications) n Stable and Predictable Low-Speed Operation (For Velocity-Sensitive Applications) n ActiveX® Programming Interfaces n Seamless Software Integration with apt™ Family
Two-Channel Stepper Motor Controller ITEM # MT-FN1
$
PRICE 4,000.00
DESCRIPTION Manual Microscope Translator
MTM-FN1
$
7,000.00
Motorized Microscope Translator with Controllers
MTME-FN1
$
11,500.00
Motorized Microscope Translator with Encoders
291
Electrophysiology Cerna Series Microscopes
U-Shaped Breadboard Features
Patch-Clamp Manipulators
n UltraLight
Series Breadboard with Sealed Holes to Contain Spills n Imperial and Metric Versions Available n Full 270° Array of 1/4"-20 (M6) Taps n Mechanically and Thermally Stable
Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
PHYS24BB
The PHY24BB(/M) breadboard is designed to be used as a microscopy stage; the U-shape surrounds the microscope providing 270˚ of workspace with over 200 1/4"-20 (M6) tapped mounting holes. Its lightweight aluminum construction makes it portable while the thermally stable honeycomb construction provides excellent dynamic rigidity with a high strength-to-weight ratio. In addition, tapped mounting holes are individually sealed in order to contain spills. SPECIFICATIONS The dimensions of the U-shaped breadboard Dimensions 17.72" x 23.62" x 0.98" (650 mm x 450 mm x 25 mm) are ideal for the Nikon Eclipse FN1 upright Weight 15.5 lbs (7 kg) microscope. The Ø110 mm insert seen in the Load Capacity 110 lbs (50 kg) picture above can be removed entirely or it can be Tapped Hole Matrix 1/4"- 20 (M6) Tapped Holes over 270° Surface used to hold a standard microscope slide or 35 mm Petri dish. Larger specimens, patch clamps, and other accessories can be easily secured to the working surface using the 1/4"-20 (M6) tapped holes. ITEM # PRICE 2,500.00 PHYS24BB $
DESCRIPTION U-Shaped Breadboard for Physiology Stage, 1/4"-20 Taps
PHYS24BB/M $ 2,500.00
U-Shaped Breadboard for Physiology Stage, M6 Taps
Adjustable-Height Support Columns The PHYS12P(/M) Adjustable-Height Support Columns are designed to support a breadboard or microscope stage. Each post has a top-located 1/4"- 20 (M6 x 1.0) tap and a universal base that has four 1/4" (M6) counterbored holes on 2" (50 mm) centers for mounting it to an optical table or breadboard. The height of the post is continuously adjustable from 8" to 12", and its position can be locked using a knurled ring locking collar. To secure the columns to an optical table is as simple as loosening the locking collar and raising the red housing. This will provide access to the four counterbored 1/4" (M6) mounting holes.
Features n Continuously
Adjustable Height from 8" to 12" n Lockable Design n Top-Located 1/4"-20 (M6) Tap n Universal Base for Mounting to Work Surfaces with Imperial or Metric Taps n Load Capacity/Post: 300 lbs (136 kg)
ITEM # PRICE 450.00 PHYS12P $ PHYS12P/M $ 450.00
292
PHYS12P Height can be Adjusted from 8" to 12"
DESCRIPTION Adjustable-Height Support Column, 8" to 12" (1/4"-20 Tap) Adjustable-Height Support Column, 8" to 12" (M6 Tap)
Electrophysiology
Manual XY Stage for the Nikon FN1 Microscope
Cerna Series Microscopes
The MPM-XYRS manual XY stage is designed for use with the Nikon FN1 MPM-XYRS microscope (see image to Mounted on a MPM-XYRS Nikon FN1 Nikon FN1 XY Stage the right) during physiology Microscope experiments. It provides an M6-tapped hole matrix on 25 mm centers on either side of a central sample plate. The tapped holes enable the user to mount appropriate hardware close to the specimen.
Patch-Clamp Manipulators
The translation is facilitated through tension adjusters with 30 mm translation in X and Y. This method of translating the specimen under the field of view of the microscope enables viewing of a large area of the sample. ITEM # MPM-XYRS
$
PRICE 2,845.00
Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
DESCRIPTION Manual XY Stage for Nikon FN1 Microscopes
Have you seen our...
Bergamo II Series Multiphoton Microscopes ◆ Z, XYZ, or XYZ + Ѳ Motorization Levels ◆ Super Broadband Scan Optics ◆ Two or Four Non-Descanned PMT Detectors Designed to be integrated with your research, the Bergamo II Series can be readily configured or reconfigured to meet a range of imaging requirements from imaging cells to larger animals. Our extended-field-of-view detector modules maximize the collection of the fluorescence signal from in vivo images, even when the fluorescence is scattered through hundreds of microns of tissue.
Bergamo II Series Rotating Microscope Configuration
Users can track multiple-labeled specimens by employing up to four ultrasensitive GaAsP PMTs. Capture intracellular dynamics at up to 400 frames per second using our advanced resonant scanner technology. The Bergamo II Microscopes incorporate proprietary extendedwavelength excitation optics, making it possible to take full advantage of the ever-evolving spectrum of fluorescent proteins.
See Pages 2 - 35 293
Electrophysiology Cerna Series Microscopes
Motorized Platforms
Patch-Clamp Manipulators
Features
Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
Low-Drift, High Resolution Platforms for Electrophysiology Applications n Motorized Control for Smooth and Precise Movement n Large and Small Platform Options: • Large Platforms for Physiology Tabletop • Small Platforms for Rigid Stands (See Pages 296 - 300) n
Physiology Stages
PMP-2XY U-Shaped Standard Platform
MCM3000 3-Axis Controller (Sold Separately)
Motorized U-Shaped Breadboard Thorlabs’ Motorized Platform is a large, U-shaped breadboard that provides 50 mm of travel in each direction. It is designed for systems with a static (unmovable) microscope and enables the specimen to be moved relative to the microscope objective. 352 1/4"-20 taps on 1" centers (M6 taps on 25 mm centers for the metric version) are arranged in a 270° array to offer flexibility to mount hardware in the optimal location for experimental demands. This motorized platform installs easily around any existing microscope or can be used in conjunction with our Bergamo II Microscopes (see pages 2 - 35), Cerna Series Slice Rig Microscopes (see pages 270 - 273),
micromanipulators (see pages 274 - 279), or rigid stands (see pages 296 - 300) to construct a complete electrophysiology package. Each motorized platform features fast and reliable translation. Stepper motor control yields high 0.5 µm resolution and repeatability in movement, allowing the examination of multiple fields of view within the same specimen. The stage sits 4.5" above the tabletop and offers 50 mm of travel at increments of 0.05 µm/step. The platform’s base is compatible with both imperial and metric breadboards. Its functional design, ease of use, and simple integration make it an ideal and adaptable piece of hardware. The PMP-2XY Motorized Platform is incorporated into a Bergamo II Microscope with Z Motion (see page 8). Both the XY motion of the platform and Z-axis movement of the microscope are controlled from a single MCM5000 5-axis controller. Image Courtesy of Dr. Hajime Hirase and Katsuya Ozawa, RIKEN Brain Science Institute, Wako, Japan.
294
Electrophysiology Cerna Series Microscopes
Motorized Platforms Motorized Platform for Rigid Stands
PLS-XY 2D Motorized Platform for Rigid Stands
We also offer compact 1D and 2D translation stages designed for use with our rigid stands (see pages 296 - 300). These stages incorporate stepper MP100-RCH2 Slide Holder motor technology to Shown with 2D Motorized Stage provide 25 mm of travel at increments of 12 nm/step and high repeatability. The quiet and smooth action of the motors yields reliable and low-drift translation of the mounted rigid stand. When combined with the accessories for rigid stands (see pages 296 - 300), this system will yield precise control of our micromanipulator assemblies, specimen holders, or other electrophysiological hardware.
Multi-Axis Controllers
Patch-Clamp Manipulators Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
MCM3000
Thorlabs offers 3-axis and 5-axis controllers that can be used with our motorized platforms.
3-Axis Controller
MCM5000 5-Axis Controller with Touchscreen
The MCM3000 3-Axis Controller has three large control knobs. A smaller knob on the top of the unit allows the speed of movement to be adjusted continuously. The MCM5000 5-Axis Controller has four control knobs and a rocker switch to change between fine and coarse Z-control. Two spatial locations can be saved in the controller and retrieved using the integrated touch screen. Connect the controller to a computer running ThorImageLSTM to save up to eight spatial locations. Both controllers allow each axis to be individually disabled after the position is set so that alignment is maintained if the controller is accidentally bumped. When using a motorized platform and a multi-axis controller with our Bergamo II or Cerna microscopes (see pages 2 – 35 and 270 – 273, respectively), only two of the knobs (or one for the PLS-X) are used to control the platform’s motion. The remaining knob(s) can be used to control the movement of other components in the system, such as the Z-axis adjustment for the microscope.
Have you seen our...
Rigid Stands
Motorized U-Shaped Breadboard ITEM # METRIC ITEM # PMP-2XY PMP-2XY/M
PRICE $ 12,211.72
DESCRIPTION Precision Motorized Platform for Physiology Tabletop, 4.5" High
Motorized Platforms for Rigid Stands ITEM # PLS-X
PRICE $ 1,296.80
PLS-XY
$
2,370.52
DESCRIPTION 1D Motorized Platform for Rigid Stands, 1" Travel 2D Motorized Platform for Rigid Stands, 1" Travel in X and Y
Multi-Axis Controllers ITEM # MCM3000 MCM5000
$
PRICE 4,500.00
DESCRIPTION 3-Axis Controller
$
10,350.00
5-Axis Controller with Touchscreen
Available on Pages 296 - 300 295
Electrophysiology Cerna Series Microscopes
Rigid Stands
Features
Patch-Clamp Manipulators Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
Stand Heights up to 250 mm with Adjustable Platform Height n Full 360 Degrees of Rotation n Suitable for Upright and Inverted Microscopes n Quick-Lock Mechanism Allows for Easy Positioning n Solid Construction Provides Stable Mounting n Compatible with 66 mm Optical Rails and M6 Optomechanics (See Our Website) n 1D and 2D Motorized Platforms for Use with Rigid Stands n
MP100
MP150
MP200
MP250
Thorlabs’ line of rigid stands adds flexibility and adaptability into our electrophysiology structures and platforms. They are ideal for mounting our micromanipulators (see pages 274 - 279) and other electrophysiology accessories, such as slide or chamber holders. These stands include a rigid stand holder, Ø1.5" solid stainless steel post, and a 200 mm x 60 mm platform with 24 M6-tapped holes. The stand holder base has four 1/4" (M6) counterbored slots for securing it to a breadboard with 1/4"-20 (M6) cap screws. It also incorporates a height collar that is equipped with a quickrelease lock that allows for platform height adjustments over the range indicated in the price box below. When locked, the attached platform can be rotated while maintaining the desired height. The platform is secured to the Ø1.5" solid stainless steel post by a dovetail clamp, which allows the platform to be continuously adjusted in X. Two 3/32" hex cap screws on the dovetail clamp lock or release the Rigid Stands are Incorporated into horizontal movement of the platform. Bergamo II Multiphoton Systems If your application would benefit from fine motorized control in X, Y, or both axes, these rigid stands can be combined with the 1D or 2D motorized translation stages offered in the price box below, which feature an adjustment range of 25 mm with a theoretical resolution of 12 nm/step. For more details on these motorized translation stages, please see the full presentation on page 295.
Dovetail Clamp
296
ITEM # MP100
$
PRICE 530.00
DESCRIPTION Rigid Stand, Adjustment Height: 148.1 - 208.5 mm
MP150
$
543.33
Rigid Stand, Adjustment Height: 198.1 - 309.3 mm
MP200
$
556.67
Rigid Stand, Adjustment Height: 248.1 - 410.1 mm
MP250
$
570.00
Rigid Stand, Adjustment Height: 265.9 - 510.9 mm
PLS-X
$
1,296.80
1D Motorized Translation Stage for Rigid Stands
PLS-XY
$
2,370.52
2D Motorized Translation Stage for Rigid Stands
Electrophysiology Cerna Series Microscopes
Rigid Stands: Components and Accessories Rigid Stand Holders
Patch-Clamp Manipulators
LPH250
We offer our rigid stand holders separately for those wishing to use the same rigid stand with multiple holders. These holders, each of which comes with a height collar, provide a stable and rugged structure for electrophysiology needs. Each base is equipped with four 1/4" (M6) counterbored slots to secure it to a breadboard using 1/4"-20 (M6) cap screws. These holders can be used with our Ă&#x2DC;1.5" posts (available online) as well as our 1D and 2D motorized translation stages for rigid stands, which are featured on page 295.
Physiology Stages
LPH200 LPH150 LPH100
Motorized Platforms Rigid Stands Faraday Cage
ITEM # LPH100
$
PRICE 339.96
DESCRIPTION Rigid Stand Post Holder, L = 100 mm
LPH150
$
353.29
Rigid Stand Post Holder, L = 150 mm
LPH200
$
366.62
Rigid Stand Post Holder, L = 200 mm
LPH250
$
379.96
Rigid Stand Post Holder, L = 250 mm
PLS-X PLS-XY
$ 1,296.80 $ 2,370.52
EMF Shielding
L
1D Motorized Translation Stage for Rigid Stands 2D Motorized Translation Stage for Rigid Stands
Slide Holders These slide holders, which are compatible with standard microscope slides, integrate well into existing MP100-RCH2 electrophysiology systems Slide Holder or custom rigs. The rear Shown with a 2D Motorized Stage tail of the platform is held by a Ă&#x2DC;1.5" solid stainless steel post that can be rotated 360 degrees and has 15 M6-tapped holes for mounting electrophysiology hardware. The dovetail design allows the platform to be continuously adjusted in X, while rotation and vertical adjustments are controlled independently by a quick-release lock. If your application would benefit from motorized control in X, Y, or both axes, which enables finer movements, these rigid stands can be combined with the 1D or 2D motorized translation stages offered in the price box below and detailed on page 295.
MP100-RCH2 Slide Holder Shown Within a Custom Electrophysiology System
ITEM # MP100-RCH2
$
PRICE 752.91
DESCRIPTION Slide Holder, Adjustment Height: 148.1 - 208.5 mm
MP150-RCH2
$
766.24
Slide Holder, Adjustment Height: 198.1 - 309.3 mm
MP200-RCH2
$
779.58
Slide Holder, Adjustment Height: 248.1 - 410.1 mm
MP250-RCH2
$
792.91
Slide Holder, Adjustment Height: 265.9 - 510.9 mm
PLS-X
$ 1,296.80
1D Motorized Translation Stage for Rigid Stands
PLS-XY
$ 2,370.52
2D Motorized Translation Stage for Rigid Stands
297
Electrophysiology Cerna Series Microscopes
Recording Chamber Holders
Patch-Clamp Manipulators
Our recording chamber holders integrate well into existing electrophysiology systems or custom rigs. The rear tail of the platform is held by a Ă&#x2DC;1.5" solid stainless steel post that can be rotated 360 degrees and has 15 M6-tapped holes for mounting electrophysiology hardware. The dovetail design allows the platform to be MP100-RCH1 Shown with continuously adjusted in X, while rotation Custom Insert and vertical adjustments are controlled independently by a quick-release lock. The stand holder base features four 1/4" (M6) counterbored slots to secure it to a breadboard with 1/4"-20 (M6) cap screws. If your application would benefit from motorized control in X, Y, or both axes, which enables finer movements, these rigid stands can be combined with the 1D or 2D motorized translation stages offered in the price box below and detailed on page 295.
Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
MP100-RCH1 Recording Chamber Holder with Custom Insert in an Electrophysiology System
ITEM # MP100-RCH1
$
PRICE 648.22
DESCRIPTION Recording Chamber Holder, Adjustment Height: 148.1 - 208.5 mm
MP150-RCH1
$
661.56
Recording Chamber Holder, Adjustment Height: 198.1 - 309.3 mm
MP200-RCH1
$
674.89
Recording Chamber Holder, Adjustment Height: 248.1 - 410.1 mm
MP250-RCH1
$
688.22
Recording Chamber Holder, Adjustment Height: 265.9 - 510.9 mm
PLS-X
$ 1,296.80
1D Motorized Translation Stage for Rigid Stands
PLS-XY
$ 2,370.52
2D Motorized Translation Stage for Rigid Stands
Have you seen our...
Multiphoton Imaging Systems Our rigid stands are incorporated into some configurations of Thorlabsâ&#x20AC;&#x2122; Bergamo II series of multiphoton imaging systems for added versatility during in vitro or in vivo experiments. The rigid stand inserts enable the mounting of many sample types, and the platform can swing easily into and out of the microscope beam path. A minimal footprint increases the available space above and below the sample platform for simple integration into existing systems.
See Pages 2 - 35 for Details 298
Electrophysiology
Rigid Stand Inserts and Holders
Cerna Series Microscopes
Thorlabs’ Rigid Stand Insert Holders are large, empty platforms available in four heights (100 mm, 150 mm, 200 mm, or 250 mm) for easy customization of electrophysiology systems. Each insert holder can be paired with any one of seven inserts with options such as breadboard platforms, slide holders, or piezoelectric stages. Like all our rigid stand products, these platforms include a Ø1.5" post and a stand holder base. The dovetail mechanism allows the platform to be continuously adjusted in X, while rotation and vertical adjustments are controlled independently by a quick-release lock.
Patch-Clamp Manipulators
MP100-MLSH Insert Holder
The insert holders themselves are designed to accommodate any of the inserts sold here and feature eight M6-tapped holes and four M6 (1/4") counterbores. The inserts are held in place by four 5/64" hex cap screws and can also fit into the MZS500-E Z-Axis Stage. If your application would benefit from finer motorized control in X, Y, or both axes, these rigid stands can be actuated using either our 1D or 2D motorized rigid stand translation stages.
Physiology Stages Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
Blank Platform n Ideal
for Custom or Non-Standard Applications n Easily Drilled and Tapped to Create Customized Electrophysiology Systems MP100-MLSH Insert Holder Shown with Blank Adapter Plate
MLS203P3 Blank Adapter Plate
Breadboard Platform n 35
MP100-MLSH Insert Holder Shown with MLS203P4 Breadboard Plate
Tapped Holes • 1/4"-20 (MLS203P5) on 1" Centers • M6 (MLS203P4) on 25 mm Centers n Flexible Mounting Options for Electrophysiology Hardware MLS203P4 M6-Tapped Breadboard Plate
Slide/Petri Dish Holder for Inverted Microscopes n
ompatible with 1" x 3" and C 25 mm x 75 mm Microscope Slides as well as Ø30 mm to Ø60 mm Petri Dishes
MP100-MLSH Insert Holder Shown with Slide/Petri Dish Holder and 2D Motorized Stage
MLS203P2 Slide/Petri Dish Holder
299
Electrophysiology Cerna Series Microscopes
Rigid Stand Inserts and Holders
Patch-Clamp Manipulators
Multi-Slide Holder for Upright or Inverted Microscopes
Physiology Stages
n Compatible
Motorized Platforms
MP100-MLSH
Rigid Stands
Insert Holder shown with Multi-Slide Holder for Upright or Inverted Microscopes
Faraday Cage
with 1" x 3" and 25 mm x 75 mm Microscope Slides and 18 mm x 18 mm x 1.0 mm Calibration Targets n Holds up to Three Slides and One Calibration Target MLS203P9 Multi-Slide Holder for Upright or Inverted Microscopes
EMF Shielding
Recessed Slide Holder for Upright Microscopes n Allows
MP100-MLSH Insert Holder shown with Recessed Slide Holder for Upright Microscopes
the Microscope Condenser to be Positioned within 1.5 mm (0.06") of the Slide Surface n Compatible with 1" x 3" and 25 mm x 75 mm Microscope Slides and 18 mm x 18 mm x 1.0 mm Calibration Targets MLS203P11 Recessed Slide Holder for Upright Microscopes
Z-Axis Piezo Stage Holder n Z-Axis
Piezo Stage and Controller Âľm of Vertical Travel n 25 nm Resolution n 500
MZS500-E Z-Axis Piezo Stage and Controller
MP100-MLSH Insert Holder Shown with Z-Axis Piezo Stage
Choose the Desired Rigid Stand Height ITEM # MP100-MLSH
$
PRICE 769.84
DESCRIPTION Large Insert Holder, Adjustment Height: 148.1 - 208.5 mm
MP150-MLSH
$
783.18
Large Insert Holder, Adjustment Height: 198.1 - 309.3 mm
MP200-MLSH
$
796.51
Large Insert Holder, Adjustment Height: 248.1 - 410.1 mm
MP250-MLSH
$
809.84
Large Insert Holder, Adjustment Height: 265.9 - 510.9 mm
Choose the Desired Insert ITEM # MLS203P3
$
PRICE 150.00
DESCRIPTION Blank Adapter Plate
MLS203P5
$
200.00
MLS203P4
$
200.00
Metric M6 Breadboard Plate
MLS203P2
$
450.00
Slide/Petri Dish Slide Holder for Inverted Microscopes
MLS203P9
$
460.00
Multi-Slide Holder for Upright or Inverted Microscopes
MLS203P11
$
395.00
Recessed Slide Holder for Upright Microscopes
MZS500-E
$
10,495.00
Imperial 1/4"-20 Breadboard Plate
Z-Axis Piezo Stage and Controller Kit
Motorized Translation (Optional)
300
ITEM # PLS-X
$
PRICE 1,296.80
DESCRIPTION 1D Motorized Translation Stage for Rigid Stands
PLS-XY
$
2,370.52
2D Motorized Translation Stage for Rigid Stands
Electrophysiology Cerna Series Microscopes
Free-Standing Faraday Cage Enclosure
Patch-Clamp Manipulators
Copper Mesh Shielding Effectiveness
Attenuation (dB)
100 90
Physiology Stages
70
Motorized Platforms
50
Rigid Stands
30
FAR05F Free-Standing Faraday Cage
10
Faraday Cage 0
40
80 120 Frequency (MHz)
160
200
EMF Shielding
Experiments in electrophysiology, confocal microscopy, and other sensitive applications must often be shielded from external interferences such as electrostatic fields and high frequency electromagnetic waves. Such disturbances can be caused by AM/FM radio waves, CRT oscilloscopes, and fluorescent light strips. Faraday cages minimize the electromagnetic waves that interfere with an object placed within them. Waves that are significantly longer than the mesh spacing will be blocked. Thorlabs offers both standalone and workstation-based (see page 302) versions of its Faraday Cage Enclosure. The FAR05F Free-Standing Enclosure featured here can be placed on any tabletop surface and has a 16 count copper mesh, offering a shielding effectiveness of 55 dB (at 10 MHz). Experiments within the cage can be easily accessed by lifting away the cage’s panels, which are magnetically secured to the frame. This standalone enclosure is compatible with Thorlabs’ 25 mm Optical Construction Rails using the PSY404 Rail System Adapter Kit. By attaching one of the included adapter plates to each end of a user-supplied 25 mm optical construction rail, the rail can be mounted perpendicular to a segment of the Faraday cage’s frame as shown in the photo to the right. Visit our website to see our 25 mm Rail product offering. Two feed-through ports on the back panel offer Ø1.75" (Ø44.4 mm) clearance. Additional ports can be added by purchasing one or more of the PSY405 Cable Port Kits.
The PSY405 Port Kit Allows for the Addition of Port Holes to Any of the Panels on a Faraday Cage
Step #1: Add the Free-Standing Faraday Cage Enclosure ITEM # FAR05F
DESCRIPTION Free-Standing Faraday Enclosure
$
PRICE 2,310.00
$
PRICE 40.00
$
PRICE 22.00
Step #2: Do You Wish to Use 25 mm Rails Within the Enclosure? ITEM # PSY404
DESCRIPTION Faraday Enclosure XE25 Rail Adapter Kit
Step #3: Do You Need to Add Any Additional Port Holes? ITEM # PSY405
DESCRIPTION Faraday Enclosure Cable Port Kit
301
Electrophysiology Cerna Series Microscopes
Faraday Cage Workstation
Patch-Clamp Manipulators
Features Isolates Experiments from Disruptive Electromagnetic Waves n Shielding Effectiveness of 55 dB at 10 MHz n Removable Magnetic Panels for Quick Access to Experiment n Accessories Fit Inside and Around the Workstation n Compatible with our 750 mm × 900 mm ScienceDesk Frame
Physiology Stages
n
Motorized Platforms Rigid Stands Faraday Cage EMF Shielding
Copper Mesh Shielding Effectiveness 100
Attenuation (dB)
90 70 50 30 10
0
40
Cable Port Kit (See Next Page) Monitor Mount Attached with Post Adapter (See Next Page) Side Shelf Adapter (See Next Page)
Faraday Cage Workstation Shown with Optional Accessories
In addition to the Free-Standing Faraday Cage featured on the previous page, Thorlabs also offers a Faraday Cage Workstation that mounts onto a ScienceDesk frame. The enclosure utilizes the same 16 count copper mesh as its standalone counterpart, providing a shielding effectiveness of 55 dB (at 10 MHz). Since the enclosure mounts to the ScienceDesk as opposed to the breadboard, the enclosure does not interfere with the vibration-isolated tabletop.
The work surface measures 2.5' x 3' (750 mm x 900 mm) and sits inside the Faraday cage. Choose from a tabletop that either has no holes or provides 80 120 160 200 sealed 1/4"-20 (M6) mounting holes on 1" (25 mm) centers with a 0.5" Frequency (MHz) (12.5 mm) border at the edge of the table. The Faraday Cage Workstation provides a complete mounting and shielding solution for our Gibraltar and Physiology stages (see pages 280 289). Regardless of the tabletop chosen, all Faraday Cage Workstations come with active, self-leveling isolators to provide the highest degree of vibration isolation. Ideal for imaging applications with sub-micron resolution, these isolators require a constant source of pressurized air, such as that provided by our PTA512 Air Compressor. An array of shelving options, including overhead, instrument, and under shelves, can be mounted within and around the cage as shown in the photographs above and on the next page. Please note that an adapter is needed to attach the shelves to the ScienceDesk frame (see the price box for details). Experiments within the cage can be easily accessed since the cage’s panels are magnetically secured to the frame and can be lifted away. Two feedthrough ports on the back panel offer Ø1.75" (Ø44.4 mm) clearance. Additional ports can be added by purchasing one or more PSY405 Cable Port Kits. This Faraday Cage Workstation is also compatible with Thorlabs’ 25 mm Optical Construction Rails using the PSY404 Rail System Adapter Kit. By attaching one of the included adapter plates to each end of a user-supplied 25 mm optical construction rail, the rail can be mounted
302
Panels are Secured to the Cage Frame Using Magnets
Electrophysiology
Faraday Cage Workstation
Cerna Series Microscopes
perpendicular to a segment of the Faraday cage’s frame as shown in the photo at the top of the previous page. Visit our website to see our 25 mm Rail product offering.
Patch-Clamp Manipulators Physiology Stages Motorized Platforms
PSY221
PSY401
Side Shelf Attached to the ScienceDesk Using a PSY401 Side Shelf Adapter
Overhead Shelf
Adapter with PSY350 Side Shelf
Rigid Stands
PSY350
Faraday Cage EMF Shielding
PSY191 Instrument Shelf
PSY403
PSY311 Under Shelf
Adapter Shown with PSY161 Post and PSY121 Monitor Mount (Not Included)
Step #1: Add the Faraday Cage Enclosure ITEM # FAR01
DESCRIPTION Faraday Enclosure for a 750 mm x 900 mm ScienceDesk Frame
$
PRICE 2,510.00
$
PRICE 2,500.00
Step #2: Add the Active, Self-Leveling Vibration Isolation ScienceDesk Frame ITEM # SDA7590
ACCEPTED TABLETOP SIZE 2.5' x 3' (750 mm x 900 mm)
FRAME WEIGHT 165 lbs (75 kg)
Step #3: Add an Air Compressor (Choose One) ITEM # PTA512
DESCRIPTION Air Compressor, 110 / 115 V, 60 Hz, US Power Plug
$
PRICE 600.00
PTA513
Air Compressor, 220 / 240 V, 50 Hz, UK Power Plug
$
600.00
PTA514
Air Compressor, 220 / 240 V, 50 Hz, EU Power Plug
$
600.00
Step #4: Add a Tabletop (Choose One) ITEM # B3036T
DIMENSIONS 30" x 36" x 2.4"
MOUNTING 1/4"-20 Mounting Holes, Sealed
$
PRICE 1,305.82
B7590L
750 mm x 900 mm x 60 mm
M6 x 1.0 Mounting Holes, Sealed
$
1,305.82
B3036Y
30" x 36" x 2.4"
No Mounting Holes
$
1,041.67
$
PRICE 50.00
Air Compressor
Step #5: Do You Need Any Adapters for Use With the Faraday Cage? ITEM # PSY403
DESCRIPTION Faraday Enclosure Single External Post Adapter
PSY401
Faraday Enclosure Post and Side Shelf Adapter
$
130.00
PSY404
Faraday Enclosure XE25 Rail Adapter Kit
$
40.00
$
PRICE 22.00
Step #6: Do You Need to Add Any Additional Port Holes? ITEM # PSY405
DESCRIPTION Faraday Enclosure Cable Port Kit
Step #7: Do You Want Any Shelves? ITEM # PSY191*
DESCRIPTION Instrument Shelf
PSY191/S
Additional Instrument Shelf
PSY192*
Instrument Shelf
PSY192/S
Additional Instrument Shelf Under Shelf
PSY311
SHELF DIMENSIONS 11.8" x 10.9" (300 mm x 278 mm)
LOAD CAPACITY (MAX) 44 lbs (20 kg)
PSY161 POST NEEDED 1 (Included)
11.8" x 10.9" (300 mm x 278 mm)
44 lbs (20 kg)
1 (Not Included)
$
115.00
11.8" x 19.7" (300 mm x 500 mm)
44 lbs (20 kg)
1 (Included)
$
187.00
11.8" x 19.7" (300 mm x 500 mm)
44 lbs (20 kg)
1 (Not Included)
$
125.00
30" x 36" (750 mm x 900 mm)
220 lbs (100 kg)
–
$
210.00
$
PRICE 177.00
PSY221*
Overhead Shelf
37.64" x 11.81" (956 mm x 300 mm)
110 lbs (50 kg)
2 (Included)
$
355.00
PSY223
Additional Overhead Shelf
37.64" x 11.81" (956 mm x 300 mm)
110 lbs (50 kg)
2 (Not Included)
$
187.00
Side Shelf
23.6" x 29.5" (750 mm x 600 mm)
110 lbs (50 kg)
–
$
175.20
PSY350**
*Requires the Purchase of either the PSY401 or PSY403 Adapter if Being Installed Outside the Faraday Cage **Requires the Purchase of the PSY401 Adapter to attach the Shelf to the ScienceDesk
303
Electrophysiology Cerna Series Microscopes
Metallic Foil for EMF Shielding
Patch-Clamp Manipulators
Features
Physiology Stages
n P rotect
Sensitive Electronic Equipment from Strong Magnetic Fields n Combine High- and LowPermeability Foils for Additional Magnetic Shielding Options n Pressure-Sensitive Adhesive Backing for Easy Installation
Motorized Platforms Rigid Stands Faraday Cage
MSFHP
EMF Shielding
Thorlabs’ Magnetic Shielding Foil provides effective protection for setups with sensitive electronic equipment whose normal operation can be affected by electromagnetic interference (EMI) and strong external magnetic fields. We offer both high- and low-permeability metallic shielding foils that are able to absorb these external magnetic fields and redirect them through the foil. The foils can be combined to create one shield that provides the widest range of magnetic shielding.
ITEM #
MSFHP
MSFLP
Physical Properties Foil Thickness
0.152 mm (0.006")
Foil Width
0.102 mm (0.004") 102 mm (4")
Roll Length
1 m (39.4") 8746.8 kg/m3 (0.316 lbs/in3)
7833.4 kg/m3 (0.283 lbs/in3)
55,000 – 75,000
300
Permeability at 100 - 200 Gauss
70,000 – 100,000
1,300
Maximum Permeability
230,000 – 300,000
3,000
Saturation Induction
7,600 – 8,000 Gauss (0.76 – 0.80 Tesla)
22,000 Gauss (2.2 Tesla)
<0.015 Oersted
1 Oersted
Foil Density Typical Shielding Properties Initial Permeability at 40 Gauss
Coercivity
Permeability Permeability of MSFLP & MSFHP
20000 18000
MSFLP MSFHP
16000
Permeability (Gs/Oe)
When exposed to a magnetic field, a material’s ability to support the formation of a magnetic field within itself can be measured. This quantity, called permeability, is given by the ratio of the magnetic flux density in the medium to the magnetic field intensity and is often expressed in units of Gauss/ Oersteds (Gs/Oe).
14000 12000 10000 8000 6000 4000 2000 0 0
2
4
6
8
Magnetic Field Intensity (Oersteds)
304
10
As shown in the graph to the left, for magnetic field intensities less than one Oersted, the MSFHP’s permeability is much greater than that of the MSFLP foil, and thus serves as the better shielding material. In contrast, for magnetic field intensities in excess of one Oersted, the MSFLP foil is the better choice, as it offers greater attenuation at these higher field intensities.
ITEM # PRICE 140.00 MSFHP $
DESCRIPTION High-Permeability Magnetic Shielding Foil, 1 m x 102 mm x 0.152 mm
MSFLP
Low-Permeability Magnetic Shielding Foil, 1 m x 102 mm x 0.102 mm
$
50.00
Electrophysiology Cerna Series Microscopes
Copper Mesh for Electromagnetic Shielding
Patch-Clamp Manipulators
Features Build Your Own Faraday Cage n 1.22 m x 1.22 m (48" x 48") Sheet n Cut to Size with Scissors n Cable Port Kit Available Below
Physiology Stages
PSY406
n
Motorized Platforms Rigid Stands Faraday Cage
This plain square-weave copper mesh is the same mesh incorporated into our Faraday Cages sold on pages 301 - 303. It can be used in electrophysiology and other sensitive application setups to minimize the influence of external interferences such as electrostatic fields and high-frequency electromagnetic waves.
EMF Shielding
Constructed from Ø0.25 mm (1/16") copper wire with 1.4 mm (0.06") spacing, this mesh offers excellent thermal and electrical conductivity and provides high air flow, making it ideal for use in Faraday Cage Construction. It can be readily soldered, is easily bent and formed into shapes, and can be cut to the desired size with scissors. Copper Mesh Shielding Effectiveness 100
SPECIFICATIONS
90
Typical Shielding Properties 57 dB
Attenuation (dB)
Attenuation at 10 MHz Physical Properties Wire Diameter
0.25 mm (1/16")
Mesh Width
1.22 m (48")
Roll Length
1.22 m (48")
Mesh Density
ITEM # PSY406
50 30 10
16 Strands/Inch
$
70
PRICE 120.00
0
40
80 120 Frequency (MHz)
160
200
DESCRIPTION Copper Mesh, 1.22 m x 1.22 m (48" x 48")
Faraday Enclosure Cable Port Kit The PSY405 cable port kit allows ports to be added to the copper mesh featured above, thereby simplify routing of electrical cables and optical fibers. Each kit enables the creation of one port with a clearance of Ø1.75" (Ø44.4 mm).
ITEM # PSY405
$
PRICE 22.00
DESCRIPTION Faraday Enclosure Cable Port Kit
305