Mycotoxin Occurrence in 2018 US Corn, Corn Silage, and Corn By-products Update Feb 1, 2019 Naturally ahead © BIOMIN America Inc. 2019
Sample Overview
7% 70% 2% 483 Samples from 30 States
9%
11%
1% Unknown Naturally ahead Š BIOMIN America Inc. 2019
Sample Overview Sample Matrix
Sampling Reason 15%
11%
13% 16%
72% 73%
Corn
Naturally ahead Š BIOMIN America Inc. 2019
Corn Silage
Corn By-product
Survey
Clinical
Unspecified
Mycotoxins & Analysis
Mycotoxin Group
Aflatoxins (AFLA)
A-Trichothecenes (A-Trich)
LC-MS/MS
B-Trichothecenes (B-Trich)
Fumonisins (FUM)
3 Labs
Naturally ahead © BIOMIN America Inc. 2019
Mycotoxins
Limit of Detection (ppb)
Aflatoxin B1
1.3
Aflatoxin B2
1.2
Aflatoxin G1
1.1
Aflatoxin G2
1.6
T-2
100.0
HT-2
100.0
Neosolaniol
100.0
Diacetoxyscirpenol (DAS)
100.0
Deoxynivalenol (DON/Vomitoxin)
100.0
Acetyldeoxynivalenol (A-DON)
100.0
Nivalenol (NIV)
100.0
Fusarenon X (FUS-X)
100.0
Fumonisin B1
100.0
Fumonisin B2
100.0
Fumonisin B3
100.0
Zearalenone (ZEN)
Zearalenone (ZEN)
Ocharatoxin A (OTA)
Ocharatoxin A (OTA)
51.7
1.1
Occurrence 100
800 88 78
% of Total Samples
80
73
72
54
52
72 500
56
52
45
42 40
39
31 20
0
24
14
318 17
19
57
8 79
471
1
2012
2013
2014
2015
# Samples per year
595
692
5
3
Naturally ahead
400 300
483 7
200 100
0
Afla
2016 B-Trich
ZEN
B-Trichothecenes include Deoxynivalenol, Nivalenol, Fusarenon-X, Acetyl-Deoxynivalenol A-Trichothecenes and Ochratoxin A are not presented due to insignificant positive samples
Š BIOMIN America Inc. 2019
49
32 27
700 600
65 60
78
2017 FUM
2018
Total Samples Analyzed/Year
81
Occurrence 2018: Corn, Corn Silage, By-products 100
93
Sample Matrix
11% 16%
73%
Corn Corn Silage
% of Total Samples
80
78
87
83
84
78
74
72
60
55 49 43
40
32
20 7
Corn By-products
10 1
0
B-Trich
FUM All
Corn
Corn Silage
ZEN Corn By-products
B-Trichothecenes include Deoxynivalenol, Nivalenol, Fusarenon-X, Acetyl-Deoxynivalenol A-Trichothecenes and Ochratoxin A are not presented due to insignificant positive samples
Naturally ahead Š BIOMIN America Inc. 2019
Afla
2
Mean of Positives 100,000 15,234
ppb (log scale)
10,000
3,878 2,738
1,000
2,056
218 100
2,093
1,438
542
489
1,682
1,190 691
2,225 927
339
287
250
3,192 1,346 353
143
109
84 51
39 20
19 11
10
2012
2013
2014 Afla
B-Trich
2015 ZEN
2016 FUM
B-Trichothecenes include Deoxynivalenol, Nivalenol, Fusarenon-X, Acetyl-Deoxynivalenol A-Trichothecenes and Ochratoxin A are not presented due to insignificant positive samples
Naturally ahead Š BIOMIN America Inc. 2019
2017
2018
100
89
84
499
387
298
353
1,135
763
1000
ppb (log scale)
3,760
2,670
1,162
1,132
1,346
10000
3,192
Mean of Positives 2018: Corn, Corn Silage, Corn By-products
3
4
10
1
B-Trich
FUM All
Corn
Corn Silage
ZEN Corn By-products
B-Trichothecenes include Deoxynivalenol, Nivalenol, Fusarenon-X, Acetyl-Deoxynivalenol A-Trichothecenes and Ochratoxin A are not presented due to insignificant positive samples
Naturally ahead Š BIOMIN America Inc. 2019
Afla
Co-occurrence 77
80
72
% of Total Samples
70 60
54
50 40
45
44 42
46
47
39
44
35 29 26
30 20
24 13
11
15
17 9
10
4
4
0
2012
2013
2014 <LOD
Naturally ahead © BIOMIN America Inc. 2019
2015 1 Mycotoxin
2016 >1 Mycotoxin
2017
2018
Co-occurrence 2018
Corn
Corn Silage
Corn By-products 5
12
2 26
26 62 72
90
<LOD
One Toxin % of Total Samples
Naturally ahead © BIOMIN America Inc. 2019
>1 Toxin
5
Co-occurrence 2018: Corn 35
33
% of Total Samples
30
25 20
19
18
15
5
10
9
10
4
2
0.3
<LOD
Naturally ahead © BIOMIN America Inc. 2019
Afla
3
0
0 ND
2
B-Trich
FUM
One toxin
ZEN
1
Afla B-Trich B-Trich Others B-Trich + B-Trich + Others + FUM + FUM + ZEN FUM + FUM + ZEN Afla Two Toxins
> 2 Toxins
Co-occurrence 2018: Corn Silage 34
% of Total Samples
35 30
25 21
21
20 15
12
10
7 4
5
1
0
0 ND
<LOD
Naturally ahead © BIOMIN America Inc. 2019
Afla
B-Trich
FUM
One toxin
ZEN
0
0
0
0
Afla B-Trich B-Trich Others B-Trich + B-Trich + Others + FUM + FUM + ZEN FUM + FUM + ZEN Afla Two Toxins
> 2 Toxins
Co-occurrence 2018: Corn By-products 90
80
% of Total Samples
80 70 60 50 40 30 20 10
5 0
0 ND
<LOD
Naturally ahead © BIOMIN America Inc. 2019
Afla
5
4
2
0
0
B-Trich
FUM
ZEN
Afla + FUM
One toxin
4
B-Trich B-Trich + FUM + ZEN Two Toxins
0
0
0
Others B-Trich + B-Trich + Others FUM + FUM + ZEN Afla > 2 Toxins
2018 Corn Risk by State: Afla n=2
n=5
n=46 1% 2
n=9
n=11 n=1 n=6 n=5
n=64
n=7 14% 53
n=1
n=1
n=12 17% 47
n=25 16% 20
n=27 7% 156
n=15 7% 25
n=21 n=2 50% 33 n=2 n=2; 50% 32
n=17
n=3 33% 1 n: # of samples submitted/state N%: % of samples tested positive/state xത : average in ppb Risk to livestock based upon threshold levels according to FDA and EU regulatory and guidance values • • • •
State with average > 20 ppb State with average < 20 ppb State with samples < LOD (1 ppb) No sample submitted Naturally ahead © BIOMIN America Inc. 2019
n=12 33% 61
n= 10 20% 35
n=21 29% 46
n=1 100% 1 n=15 n=2
20% 583
n=2 50% 46
2018 Corn Risk by State: B-Trich n=2 100% 1,173
n=5 100% 319
n=9 78% 567
n=64 98% 744
n=7 100% 845 n=1 100% 422
n=1 100% 386
n=12 58% 240
n=3 n: # of samples submitted/state N%: % of samples tested positive/state xത : average in ppb Risk to livestock based upon threshold levels according to FDA and EU regulatory and guidance values • • • •
State with average > 1,000 ppb State with average < 1,000 ppb State with samples < LOD (100 ppb) No sample submitted Naturally ahead © BIOMIN America Inc. 2019
n=46 93% 1,433
n=6 100% 2,786
n=27 81% 447
n=15 93% 513
n=25 36% 2,697
n= 10 50% 160
n=21 100% 2,931
n=2; 50% 170 n=17; 82% 510
n=21
n=12 8% 582
n=11 100% 2,435
n=2 50% 261
n=15 40% 616
n=5 100% 1,595 n=2 100% 59 n=5; 40% 278 n=1 100% 388
n=1 100% 344
n=2 100% 148
2018 Corn Silage Risk by State: B-Trich n=1 100% 891
n=1 100% 434 n=1 100% 105 n=6 83% 544
n=2
n=3 100% 596
n=16 81% 1,872
n=7 86% 738
n=2
n=3 100% 1,077 n=1 100% 891 n=16 100% 1,511 n=1 100% 227
n=3 100% 1,219 n=3 100% 740 n=1
n: # of samples submitted/state N%: % of samples tested positive/state xത : average in ppb Risk to livestock based upon threshold levels according to FDA and EU regulatory and guidance values • • • •
State with average > 1,000 ppb State with average < 1,000 ppb State with samples < LOD (100 ppb) No sample submitted Naturally ahead © BIOMIN America Inc. 2019
n=2 50% 208
n=1
2018 Corn By-Products Risk by State: B-Trich
n=1 100% 357 n=1 100% 1,920 n=2 100% 803
• • • •
State with average > 1,000 ppb State with average < 1,000 ppb State with samples < LOD (100 ppb) No sample submitted Naturally ahead © BIOMIN America Inc. 2019
n=12 92% 4,129
n=20 90% 2,540
n=6 100% 1,626
n=3 n=1 100% 100% 2,523 3,814
n=2 50% 122
n=1 100% 1,471
n: # of samples submitted/state N%: % of samples tested positive/state xത : average in ppb Risk to livestock based upon threshold levels according to FDA and EU regulatory and guidance values
n=2 100% 1,497
n=1 100% 1,172
n=3 100% 3,885
2018 Corn Risk by State: FUM n=2
n=5 20% 100
n=9 89% 399
n=64 67% 912
n=7 71% 216
n=1 100% 5,600
n=1
n=12 100% 11,617
n=3 n: # of samples submitted/state N%: % of samples tested positive/state xത : average in ppb Risk to livestock based upon threshold levels according to FDA and EU regulatory and guidance values • • • •
State with average > 2,000 ppb State with average < 2,000 ppb State with samples < LOD (100 ppb) No sample submitted Naturally ahead © BIOMIN America Inc. 2019
n=46 50% 765
n=12 75% 2,667
n=25 96% 8,804
n= 10 100% 9,930
n=11 82% 544
n=6 33% 250 n=15 87% 1,023
n=27 85% 1,847
n=21 86% 408
n=2; 100% 1,250
n=17; 100% 6,635 n=21 100% 2,386
n=2 100% 3,850
n=5 80% 290 n=2 100% 7,050 n=5;100% 11,060
n=1 100% 1,400 n=15 100% 6,773
n=1 100% 2,600
n=2 100% 3,150
2018 Corn Silage Risk by State: FUM n=1
n=3
n=16 6% 200
n=1
n=1
n=6
n=2 100% 350
n=7 43% 167
n=2
n=3
n=1 100% 100 n=16 56% 1,378
n=1
n=3 33% 400 n=3 100% 333 n=1
n: # of samples submitted/state N%: % of samples tested positive/state xത : average in ppb Risk to livestock based upon threshold levels according to FDA and EU regulatory and guidance values • • • •
State with average > 2,000 ppb State with average < 2,000 ppb State with samples < LOD (100 ppb) No sample submitted Naturally ahead © BIOMIN America Inc. 2019
n=2 50% 800
n=1
2018 Corn By-Products Risk by State: FUM
n=2 100% 300
n=12 83% 1,020
n=1
n=1 100% 4,900 n=2 100% 200
• • • •
State with average > 2,000 ppb State with average < 2,000 ppb State with samples < LOD (100 ppb) No sample submitted Naturally ahead © BIOMIN America Inc. 2019
n=6 100% 1,033
n=1 100% 1,100
n=2 100% 250
n=1 100% 300
n: # of samples submitted/state N%: % of samples tested positive/state xത : average in ppb Risk to livestock based upon threshold levels according to FDA and EU regulatory and guidance values
n=20 85% 1,247
n=1 100% 300
n=3 67% 850
n=3 100% 2,367
2018 Corn Risk by State: ZEN n=2 100% 251
n=5 20% 591
n=9 33% 482
n=64 67% 323
n=7 71% 216
n=1 100% 157
n=1
n=46 70% 337
n=12 8% 81
n=11 91% 292
n=6 83% 187
n=27 44% 131
n=21 86% 408
n=15 13% 167
n=25 4% 137
n=2 n=5
n: # of samples submitted/state N%: % of samples tested positive/state xത : average in ppb Risk to livestock based upon threshold levels according to FDA and EU regulatory and guidance values • • • •
State with average > 100 ppb State with average < 100 ppb State with samples < LOD (51.7 ppb) No sample submitted Naturally ahead © BIOMIN America Inc. 2019
n=21
n=12 8% 388
n=1
n= 10
n=2
n=15 7% 64
n=1
n=2
n=2
n=17; 47% 196
n=3
n=5 80% 290
2018 Corn Silage Risk by State: ZEN n=1 100% 290
n=3 100% 68
n=16 63% 427
n=1
n=1 100% 60 n=6 n=2
n=7 71% 336
n=2 17% 4
n=3 67% 327 n=1 100% 126
n=16 69% 503 n=1 100%; 55
n=3 100% 800 n=3 67% 201 n=1
n: # of samples submitted/state N%: % of samples tested positive/state _: average in ppb Risk to livestock based upon threshold levels according to FDA and EU regulatory and guidance values • • • •
State with average > 100 ppb State with average < 100 ppb State with samples < LOD (51.7 ppb) No sample submitted Naturally ahead © BIOMIN America Inc. 2019
n=2 n=1
2018 Corn By-Products Risk by State: ZEN
n=2 100% 138
n=12 92% 637
n=1
n=1 100% 239 n=2 50% 74
• • • •
State with average > 100 ppb State with average < 100 ppb State with samples < LOD (51.7 ppb) No sample submitted Naturally ahead © BIOMIN America Inc. 2019
n=6 100% 387
n=3 n=1 100% 100% 310 295
n=2
n=1 100% 146
n: # of samples submitted/state N%: % of samples tested positive/state _: average in ppb Risk to livestock based upon threshold levels according to FDA and EU regulatory and guidance values
n=20 80% 630
n=1 100% 135
n=3 100% 487
2018 Corn Survey Summary Corn
Corn Silage
Corn By-product
98% Positive vs. 89% in 2017
98% Positive vs. 91% in 2017
95% Positive vs. 100% in 2017
72% >1 MTX vs. 47% in 2017
72% >1 MTX vs. 30% in 2017
90% >1 MTX vs. 94% in 2017
B-Trich: 74% vs 70%
B-Trich: 83% vs 85%
B-Trich: 93% vs 99%
FUM: 78% vs 52% ZEN: 43% vs 25%
FUM: 32% vs 12%
FUM: 87% vs 91% ZEN: 84% vs 80%
ZEN: 55% vs 26%
Forecast for livestock production in 2019 MED to HIGH risk Naturally ahead © BIOMIN America Inc. 2019
Questions? raj.murugesan@biomin.net
erika.hendel@biomin.net
paige.gott@biomin.net
Thank You Naturally ahead Š BIOMIN America Inc. 2019