Basic Steps Collection & Pasteurisation The first step is to obtain the substrate. In this case I have collected substrate from the waste stream. Once collected it is vital to ensure that the substrates are pasteurised to avoid contamination of unwanted mould and bacteria.
Inoculation Add the ‘activated’ or woken up mycelium to the substrate. This can come in the form of mycelium straight from an agar culture or from grain spawn. The ratio will depend on the substrate and on the mycelium used.
Growing Leave the mycelium to grow around the substrate. This stage can take anywhere from 5 days to two weeks depending on many factors and conditions like substrate, type of mushroom, moisture levels, amount of spawn etc.
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