Appk thenextstep v24092013

Applikon Biotechnology | The Next Step

Content

The Next Step 04 The Building 06 Company Structure 13 From Past to Present 14 Our Products 24

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Applikon Biotechnology | The Next Step

The Next Step Applikon Biotechnology is a world leader in developing and supplying advanced bioreactor systems from laboratory scale to production scale. We are the only company that can take a customer from the initial screening stage up to full-scale production using the same platform.

This minimizes scale-up risks and guarantees the shortest time to market for our customers’ new product development. Applikon is known for bringing new technologies to the market.

These new technologies offer advantages in process efficiency for research and development as well as pilot plant and production scale processes.

As a focused mid sized company we live by our motto

systems generating results that mimic production scale.

‘A Step Ahead’ to differentiate ourselves from other suppliers.

At the moment there are thousands of Applikon bioreactors

Our sole focus on developing bioreactor systems, allows us

used in the world from small scale R&D up to full scale

to produce state-of-the-art equipment. This has resulted in a

cGMP production.

steady growth to the top position of the worldwide laboratory

Since we started in 1974 we have shown a healthy growth

bioreactor market. The basis for our new product development

and profit. R&D is done in house in our headquarters in Delft,

lies in our strong cooperation with leading international

The Netherlands. Design and engineering is done in house but

universities and institutes.

manufacturing parts is outsourced. Final assembling is done

As a privately owned company our focus is not on short-term

in-house as well as the final testing and documentation.

shareholders value but on building a healthy company by

We have our own sales and service organizations in the

supplying the best product offering for our customers now

USA (Foster City, California and Dover, New Jersey), the UK

and in the long-term future. This is the basis of many

(Tewkesbury) and China (Guangzhou). We have well trained

long-term relations with our clients all over the world.

local distributors for sales and service in over 25 countries.

All our efforts are focused on supplying the best solution

We are continuously executing strategic plans to grow the top

for our clients in the biotech and pharmaceutical industry.

line and strengthen our financial performance. New and ongoing

Validation and documentation is a vital part of our

programs are exploring new markets, developing innovative

development procedures and this is extended throughout

products, improving service to customers, increasing efficiency,

our whole company.

and reducing costs. In line with this continious development we

Applikon Biotechnology is unique in the mini and micro

recently moved the company into our own new facility in Delft.

bioreactor range. No other company can offer a complete

Our mission: ‘Providing reliable solutions for the bioprocess

solution on this very small scale. We develop small scale

market that will enable an improved quality of life’.

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Applikon Biotechnology | The Next Step

Applikon Biotechnology moves to Biotech Campus Delft1, Netherlands The new state of the art facility of Applikon Biotechnology is located at the Science Park Technopolis in Delft, one of the five locations of Science Port Holland.

The building provides Applikon with a floor area of 3,700 m², in a unique combination of office, laboratory and production space.

1) Biotech Campus Delft consists of two locations: Technopolis and the DSM site. The sites are created to encourage (biotech) innovations, to realize a significant reduction in CO2 emissions while stimulating the economy.

Moreover, the international reputation of TU Delft and DSM in the field of industrial biotechnology are a basis for the two sites. One location is the site of the new building of LaB6 DSM and the other location is Technopolis.

Cepezed architects have designed the facility, in partnership with Smit Westerman and Tree – Delft.

Heembouw constructed the building.

The unique floor and ceiling construction with integrated (air) channels and piping ensures optimum floor height within a limited space. The low positioning of the open elements in the frontage of the building creates a pavilion-like atmosphere with optimal view from the workplaces. The entrance features a fully glazed facade from the ground floor to the top floor, this transforms the area in a pleasant informal meeting space.

In addition, the building has a spacious roof terrace that is completely surrounded by a green roof garden.

Climate control in the building is realized by means of an energy efficient thermal storage system. With this system, ground water bearing layers in the earth are used for storing excess heat in summer and excess cold in winter. The excess heat stored in summer is used to heat the building in the winter and the excess stored cold is used in summer to cool the building. Such a thermal storage system is an efficient and sustainable way for climate control in the building. The thermal comfort system consists of 3,250 m² floor/ceiling heating and cooling.

Our new facility reflects the quality and the advanced technology of the products that Applikon Biotechnology brings to the market, in a perfect way.

Applikon Biotechnology | The Next Step

In the weekend of August 16th 2013 Applikon Biotechnology has moved into their new high-tech facility in Delft. The complete move took just 3 days, which is an example of the efficiency and focus that the company can realize when large projects need to be completed in a limited time.

The new facility is a building that reflects the quality and the advanced technology of the products that Applikon Biotechnology brings to the market and provides a strong base for future growth.

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Applikon Biotechnology | The Next Step

Applikon Biotechnology | The Next Step

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Applikon Biotechnology | The Next Step

Company Structure Applikon Biotechnology pursues a policy of keeping corporate staff structure lean.

We have our own companies in the Netherlands, USA, UK and China.

The Dutch, US and Chinese companies function as local support centers for the Applikon Biotechnology distributors in the region. Each of these companies have their local warehouse, assembling, training facilities, sales and technical service and support staff.

Applikon Biotechnology has over 25 specialized local distributors for sales & service in the main biotech markets in the world.

a step ahead

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Applikon Biotechnology | The Next Step

From Past to Present 1970 Pieterman hardglas hires Mr. Jan van Burg in the technical scientific glass department. This is the start of what later will become Applikon Biotechnology. Pieterman is an industrial glass manufacturer.

1973 Applikon BV was founded in 1973 as a subsidiary of Pieterman BV.

1974 In 1974 Applikon starts her activities by selling the technical products of Pieterman, the so-called Technical Scientific equipment. The core products are the electro-chemical laboratory analysis instruments of the Swiss Company Metrohm AG. Besides these products, Applikon also represents products of other foreign firms, mainly products based on Biotechnology. Three groups of specialists support these activities, application research, training and project group. This strategy is also anchored in the company name: “Appli” is deducted from the English “to apply”; and the last part “kon” is used in a lot of city names in Switzerland. Applikon is located in Vlaardingen, Holland.

Applikon Biotechnology | The Next Step

New cars and their proud owners at Applikon, George Stephensonweg 10, Vlaardingen Holland

John the Alchemist

Yes! We got that order!

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1978

Applikon Biotechnology | The Next Step

In Schiedam a new building is the home base of Applikon. Besides the development of special analyzer equipment for the environmental and the process industry, Applikon starts with the development of small bioreactor systems and control equipment for the biotechnology market in the Netherlands and Germany.

, Jan van Burg s son Jan Paul helps hitting the first concrete foundation pole into the ground for the new building in Schiedam.

Applikon Biotechnology | The Next Step

The first 2 liter fermentor system

Bringing dreams to life

The first 3 liter fermentor system for Gist Brocades

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Applikon Biotechnology | The Next Step

Workspaces with drawing boards and the first computers.

ve Roa d A ppli ko n a t Beeca A A us tin Texas US

1983 The sales activities of the bioreactor system is expanding into other European countries and USA. Especially in the USA, the biotechnology sector is booming. This leads to an American subsidiary of Applikon in Austin, Texas.

Joh n

Applikon Biotechnology | The Next Step

1984 etrohm In 1984 M on lik and the App in the ga management ikon. pl shares of Ap

The fir st ADI 1020

Fase 2, De Brauwweg 13, Schiedam

1985 The building structure in Schiedam is extended to 2000 m2.

The biotechnology activities are put into a new founded company Applikon Dependable Instruments. In Situ Sterilizable systems (SIP) and microprocessor instrumentation for control are successfully added to the product line.

The ADI 1020 Bio Processor is introduced to the market.

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Applikon Biotechnology | The Next Step

72 liter in-situ bioreactor system

1988

Applikon Inc. Chess Dri ve Foster Ci ty USA

On request of a number of large customers in the USA, Applikon moves its activities to the Bay Area in San Francisco. Applikon Inc. moves to Foster City, California. New developments are started for extending the product program.

1989 The bioreactor program of Contact Flow in Ridderkerk is acquired. Applikon gains the technology of the production of child vaccines that was developed with the Dutch Vaccine Institute in Bilthoven.

1990 The premises in Schiedam is now increased to 10,000 m2.

Another company, Instrumek BV bases its company on the

Bu rg in 1989 Appl ikon’s CE O Ja n va n

same area and ADI assumes part ownership of Instrumek.

Instrumek is specialized in parts for the biotechnology as an analyzer equipment.

Applikon Biotechnology | The Next Step

1992 In collaboration with DHV, Amersfoort and the RIVM, Bilthoven, Applikon is awarded an order from the Chinese government to build three large-scale human vaccine manufacturing plants in China. By securing and successfully completing this project, Applikon becomes an important supplier in the field of large-scale vaccine production plants.

1998

Bioreacto r systems ready to ship to E li Lilly Liverpool UK (yr 20 00) The biotechnology division continues to develop.

This leads to a new division, AppliSens.

AppliSens specializes in the field of sensor technology and special separation systems for perfusion cell cultures.

Applikon Biotechnology is awarded ISO 9001 certification.

2002

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Applikon Biotechnology | The Next Step

2004 Applikon Biotechnology BV is split off as a separate company from Applikon Dependable Instruments BV and Applisens is merged into the new company.

2008 The existing management of the company Arthur Oudshoorn, Jaap Oostra and Erik Kakes acquire Applikon Biotechnology BV through a management buyout.

2009 Applikon Biotechnology signs development and supply agreement with Millipore for developing and marketing the CellReady single-use benchtop bioreactor. e x panding

3 L C ellread

y bioreactor

product assortment

2009 Applikon Biotechnology opens a Chinese branch office in Guangzhou, China.

Applikon Biotechnology | The Next Step

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2012 Applikon Biotechnology purchases the Biotech IP and products of HaloteC Instruments BV in Veenendaal Holland.

2012

Applikon signs distribution and supply agreement with HyClone Thermo Fisher, which allows mutual supply of the turn-key single use HyClone bioreactors and the Applikon control systems.

2013 Applikon Biotechnology

monitor A lcohol fermentation

opens a joint venture production facility in Guangzhou High-tech park, China.

2013

Applikon Biotechnology signs distribution and supply agreement with ATMI, which allows mutual supply of the turn-key single use PAD and iCellis nano bioreactors in combination with the Applikon control systems.

2013 Applikon Biotechnology opens a new facility and moves the company to the High-tech campus, Technopolis in Delft with about 3,700 m2 floor space.

Our Products

RAMBio,Š the high intensity mixer The RAMbioŽ is a highly-efficient mixer/incubator that dramatically enhances oxygenation and mixing for bacterial and fungal cultures.

Micro-Flask by Duetz, cultivation in microtiter plates The Micro-Flask by Duetz system facilitates reproducible and reliable culturing on microtiter plates.

Applikon Biotechnology | The Next Step

Mini Bioreactors, real small... real bioreactors The MiniBio range of bioreactors (250 ml, 500 ml and 1,000 ml total volume) is a true scale down of the laboratory scale bioreactor. The small volume reduces media costs and maximizes costly bench space.

Alcohol Fermentation Monitor, simple parallel cultivation The Alcohol Fermentation Monitor (or AFM) is a robust and user-friendly laboratory parallel fermentation system that allows for accurate comparisons of carbon conversion rates and yields for six simultaneous anaerobic fermentations.

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Applikon Biotechnology | The Next Step

Glass autoclavable bioreactors, the worldwide standard In the laboratory bioreactor and fermentor range Applikon Biotechnology is worldwide market leader because of its dependable and easy to use systems. The bioreactors and fermentors excel in quality and modularity.

Photo Bioreactors, the next best thing to sunlight The Applikon ‘PhotoBio’ photobioreactor range is based on the standard Applikon re-usable and single-use bioreactors. Special light panels are added to these systems to allow the growth of photosynthesizing organisms (plants, algae, bacteria).

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DO2 Sensors The AppliSens Low Drift DO2-sensor is specifically designed for long-term, stable and accurate measurements in bioprocesses.

BioSep, acoustic cell retention system The Applikon BioSep system is a unique, cell retention device for high-density perfusion processes. Using high frequency resonant ultrasonic waves to separate cells instead of a physical mesh or membrane, it offers all the benefits of traditional devices but without their inherent problems and limitations.

pH Sensors The pH+ sensor has a fixed sleeve diaphragm that reduces the influence of the culture medium on the pH measurement.

Applikon Biotechnology | The Next Step

Single-use Bioreactors Applikon offers several types of Single-use Bioreactors: AppliFlex, bioreactors from 10 to 50 liter based on mixing by rocking motion, The HyClone stirred tank bioreactors in the range of 50 to 2000 liter total volume and the ATMI PadReactor and iCELLis nano Single-use Bioreactors. We can offer complete Single-use cultivation solutions from 1 liter to 2000 liter.

BioBundle, complete cultivation systems A BioBundle is a complete bioreactor system, equipped with all necessary components and is ready to use “out-of-the-box�.

Autoclavable glass bioreactor

ez-Control

AppliFlex

MiniBio

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Applikon Biotechnology | The Next Step

my-Control, color your lab my-Control is the most advanced bioreactor controller for small scale bioreactors starting at 50 ml (working volume). The system can control bioreactors up to a total volume of 3 L.

Applikon Biotechnology | The Next Step

BioXpert , supervisory control and data aquisition 速

BioXpert is a software tool to control bio-processes and to store the process data. The software is designed specifically for biotech researchers and operators in the biotech-environment.

Bench Scale and Pilot Plant steam-in-place bioreactor systems For scale up purposes, the range of the Bio Bench and Pilot Systems, designed and built to the latest standards on hygienic processing and cGMP and GAMP validation requirements, complements the laboratory scale bioreactor systems. Scale-up from laboratory scale to Pilot Plant and small scale production is simplified by the consistent bioreactor design and the scalable control solutions.

ez-Control, total control at your fingertips The ez-Control accurately controls pH, Temperature, Dissolved Oxygen, Foam/Level and Agitation in bioreactors and fermentors.

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Applikon Biotechnology | The Next Step

i-Control, the scalable control system i-Control is an easy to operate bioreactor control system and utility console based on industrial PLC’s and fieldbus I/O.

in-Control, simply powerful in-Control is a process controller for laboratory scale bioreactors.

The system is a drop-in-replacement for our older ADI 1010 Bio Controller and ADI 1030 Bio Controller.

Applikon Biotechnology | The Next Step

micro-Matrix = 24 bioreactors The next generation micro bioreactors

The unique micro-Matrix offers total control over 24 independent bioreactors in a simple microtiter plate footprint. The micro-Matrix is a true scale down of small scale bioreactors. The bioreactor square well cassette design is based upon our popular SBS-format microtiter plates that maximize mixing, optimize gas transfer, and seamlessly integrate into lab automation protocols.

BioSenz, Glucose Bio Analyzer

iLine Fast, Optical Cell Image Analyzer

The Applikon BioSenz is a unique Analyzer specifically

The iLine Fast platform is based on patented

developed for analyzing glucose in bioreactors. The heat-

Differential Digital Holographic Microscopy.

flow technology is a proprietary and patented technology for

This unique technology brings real-time label

continuous measurement with high precision and repeatability.

free monitoring to suspension cell bioreactors.

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Applikon Biotechnology | The Next Step

Special Projects, customized solutions Projects can range from very special mini bioreactors to a complete line of cGMP production systems ranging up to several thousand liters volume systems. We have developed special web based tools for communication and open information exchange within the project team.

This ensures that everybody is working with the same up-to-date information all the time.

AppliCare, complete maintenance Our Technical service and support team can help you to minimize the lost hours in your research and production schedule and minimize the costs of downtime.

a step ahead

Applikon Biotechnology BV Heertjeslaan 2 2629 JG Delft The Netherlands T. +31(0)10-208 35 55 F. +31(0)10-208 35 05 E. info@applikon-biotechnology.com

www.applikon-biotechnology.com Concept & Copy: Erik Kakes | Historic Pictures: from the archives of Jan van Burg Art Direction & Design: Jan Paul van Burg | Printed by Applikon Biotechnology 2013 Š All rights reserved | V1UA180001