BENEFICIAL EFFECT OF MILK FERMENTED WHEY AND PUMPKIN EXTRACT AGAINST AFB1 AND OTA IN VITRO A. Cimbalo, M. Frangiamone, M. Lozano, L. Escrivá, P. Vila-Donat, L. Manyes Laboratory of Food Chemistry and Toxicology. Faculty of Pharmacy. Universitat de València. Carrer Vicent Andrés Estellés s/n. 46100 Bujassot. Spain. alscim@uv.es Aflatoxin B1 (AFB1) and ochratoxin A (OTA) are mycotoxins which widely contaminate cereals and cereal-based products. However, it has been demonstrated that the use of functional ingredients can reduce mycotoxins absorption and toxicity in both humans and animals. The aim of the study was to evaluate the effect of milk fermented whey (WF) and pumpkin rich in carotenoids against AFB1 and OTA cytotoxicity in Jurkat T cells through a proteomic approach. For this purpose, cells were exposed to intestinal digests of bread contaminated with mycotoxins and enriched with 20% of pumpkin and WF, during 7 days. Moreover, a standard condition with AFB1 and OTA (100 nM) dissolved in organic solvent (DMSO) was used as control. A gel-free shotgun proteomic approach was employed to identify features across QTOF-LC/MS system. Data were analyzed with Spectrum Mill software and the differentially expressed proteins have been statistically evaluated and filtered by abundance through Mass Professional Profiler software (Agilent) (p<0.05). Bioinformatic analysis using DAVID platform showed the identification of proteins involved in several metabolic pathways, mainly in gluconeogenesis, antioxidant activity and nucleosome assembly. More specifically, histones’ expression implicated in nucleosome assembly (H2A, H2B, H2C, H3 and H4) was increased when exposing cells to functional ingredients. Furthermore, repression of cyclin A2 associated to limiting the growth of carcinogenic cells, confirmed the preventive effect of these functional ingredients. Keywords: mycotoxin, proteomics, bioactive compounds, carotenoids, QTOF-LC/MS Acknowledgements: This work was supported by the Spanish Ministry of Science and Innovation (PID2019-108070RB-I00-ALI) and the Generalitat Valenciana (PROMETEO/2018/126).
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