ISSN 2349-3720
Research & Reviews Journal of Computational
Biology (RRJoCB) sIn silico Evaluation of Îą-Amylase Inhibitory Activity of Some Commercially Available Flavonoids sIsolation of 16S rRNA and Phylogenetic Analysis of Bacillus Flexus Colonizing in Berry of Coffea arabica
Jan - April 2014
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Research & Reviews : Journal of Computational Biology
Contents
1. In silico Evaluation of รก-Amylase Inhibitory Activity of Some Commercially Available Flavonoids Arumugam Madeswaran, Kuppusamy Asokkumar, Muthuswamy Umamaheswari, Thirumalaisamy Sivashanmugam, Varadharajan Subhadradevi, Puliyath Jagannath
1
2. Isolation of 16S rRNA and Phylogenetic Analysis of Bacillus Flexus Colonizing in Berry of Coffea arabica Anupama R, Makari HK, Hosmani Shankar P 6
Research & Reviews: Journal of Computational Biology ISSN: 2349-3720 Volume 3, Issue 1 www.stmjournals.com
In silico Evaluation of α-Amylase Inhibitory Activity of Some Commercially Available Flavonoids Arumugam Madeswaran*, Kuppusamy Asokkumar, Muthuswamy Umamaheswari, Thirumalaisamy Sivashanmugam, Varadharajan Subhadradevi, Puliyath Jagannath Department of Pharmacology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore, Tamil Nadu, India.
Abstract Diabetes has become a leading killer disease in recent years. Diabetes mellitus is a metabolic disorder characterized by hyperglycemia resulting from defects in insulin secretion, insulin action or both. The primary objective of this study was to investigate the α-amylase inhibitory activity of flavonoids using in silico docking studies. In this perspective, flavonoids like apigenin, baicalin, fisetin, scopoletin and wogonin were selected. Acarbose, a known α-amylase inhibitor was used as the standard. In silico docking studies were carried out using AutoDock 4.2, based on the Lamarckian genetic algorithm principle. The results showed that all the selected flavonoids showed binding energy ranging between -6.84 kcal/mol to -5.36 kcal/mol when compared with that of the standard (-2.94 kcal/mol). Inhibition constant (9.71 µM to 116.96 µM) and intermolecular energy (-8.03 kcal/mol to -5.96 kcal/mol) of the flavonoids also coincide with the binding energy. All the selected flavonoids showed excellent α-amylase inhibitory activity when compared with the standard because of their structural properties. The α-amylase inhibitory activity of the selected flavonoids was in order of baicalin>apigenin>fisetin>wogonin>scopoletin. These molecular docking analyses could lead to the further development of potential α-amylase inhibitors for the treatment of diabetes.
Keywords: Acarbose, Diabetes, Binding energy, Inhibition constant, Intermolecular energy
RRJoCB (2014)© STM Journals 2014. All Rights Reserved
Research & Reviews: Journal of Computational Biology ISSN: 2349-3720 Volume 3, Issue 1 www.stmjournals.com
Isolation of 16S rRNA and Phylogenetic Analysis of Bacillus Flexus Colonizing in Berry of Coffea arabica Anupama R1, Makari HK2, Hosmani Shankar P3* 1
PRIST University, Vallam, Thanjuvar (TN), India IDSG Government College, Chickmagalure-577012, India 3 SBRR Mahajana First Grade College, Jayalakshmipuram, Mysore-570012, India 2
Abstract Bacillus flexus is a novel Gram-positive, alkaliphilic bacterium. It was isolated from bacterial populations in heavily infected fruits of Coffea arabica. It has the ability to synthesize active enzymes under extreme pH conditions. The 16S rDNA sequence of the organism was analyzed using BLAST program (NCBI Genbank) to verify the most closely related data base sequences. Clustal X version 1.8 was used to determine multiple sequence matches. 16S rDNA of the bacterium was amplified by PCR using inverted primer (Eubacteria specific primers). Genbank, USA has confirmed the sequence by allotting accession No. KJ459005 for the 16S rRNA sequence of Bacillus flexus. This strain has been colonizing in fruits of coffee acting as a co-infect ant, hitherto not reported.
Keywords: Bacillus flexus, Coffea arabica, 16S rDNA sequence, Genbank Ac. No KJ459005
RRJoCB (2014)Š STM Journals 2014. All Rights Reserved