World Mycotoxin Report: Impact 2019 - Webinar Presentation

WORLD MYCOTOXIN REPORT

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WORLD MYCOTOXIN REPORT: IMPACT 2019

WORLD MYCOTOXIN REPORT

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TODAY’S SPEAKERS

Alexandro Marchioro, MV, MSc Senior Mycotoxin Expert at BIOMIN

WORLD MYCOTOXIN REPORT

Martina Bellasio, Dr. Senior Mycotoxin Expert at Romer Labs

Joshua Davis, Ph.D. Communications Manager at Romer Labs

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POLL QUESTION 1 Have you found mycotoxins in your raw material or finished feed in the past 12 months? o Yes o No o Not sure

2018 REPORT IN NUMBERS BIOMIN Mycotoxin Survey 2018

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BIOMIN WORLD MYCOTOXIN SURVEY 2018

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TEN-YEAR TREND: FUMONISINS AND DEOXYNIVALENOL IN CORN (MAIZE)

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FACTORS INFLUENCING MYCOTOXIN OCCURENCE o Climate change o Drought episodes, desertification, heavy rainfall, floods o Fluctuations between wet and dry cycles o Weather and climate during growing, flowering and harvesting period

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HARVESTING CONDITIONS ARGENTINA 2017 - 2018

Font: Gazeta do Povo

Font: Notícias Agrícolas

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Font: Rio Negro Notícias

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WHAT ABOUT THE REGIONS?

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EUROPE

74%

FUM prevalence

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DON,

average contamination of 912 ppb

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NORTH AMERICA

86%

DON prevalence with an average contamination of 1853 ppb.

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70%,

FUM prevalence

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CENTRAL & SOUTH AMERICA

88%

DON prevalence with an average contamination of 1949 ppb.

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86%,

FUM prevalence

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ASIA

96%,

44%

Afla Prevalence with a maximum contamination of 697 ppb.

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FUM

prevalence with a maximum concentration of 47485 ppb.

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MIDDLE EAST

100%, FUM

contamination at an average level of 3101 ppb.

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AFRICA

84%

DON prevalence, followed by FUM with 77%.

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POLL QUESTION 2 How many mycotoxins do you test for? o o o o

0 1 2–6 6+

REGULATED MYCOTOXINS Aflatoxins Deoxynivalenol Ochratoxin A Zearalenone

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T-2 and HT-2 Toxin

Fumonisins

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EMERGING MYCOTOXINS Review on emerging mycotoxins: Gruber-Dorninger et al. 2016

EFSA Report: Ergot Alkaloids 2017

EFSA Report: Alternaria Toxins 2016 EFSA Opinion: Enniatins and Beauvericin 2014

WORLD MYCOTOXIN REPORT

EFSA Opinion: Emerging Mycotoxins 2014

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EMERGING MYCOTOXINS o Patulin: genotoxic, immunotoxic, compromises gut health o Beauvericin: adverse effects on immune system; accumulates in fat-rich tissue o Fusaric acid: negative effects on swine nervous system o Ergot alkaloids: fat necrosis, lameness, reproductive problems o Moniliformin: heart damage, genotoxic and immunosuppressive

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MASKED MYCOTOXINS

Source: biomin.net

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MASKED MYCOTOXINS

Source: biomin.net

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MASKED MYCOTOXINS

Source: biomin.net

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MYCOTOXIN CO-OCCURRENCE

A

B

Additive effect

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A+B A

B

Synergistic effect

Toxicity

A+B

Toxicity

Toxicity

Mycotoxin co-occurrence can have additive, synergistic or antagonistic effects on animals.

A+B A

B

Antagonistic effect

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MYCOTOXIN CO-OCCURRENCE

Synergistic (red line) and additive (dashed line) effects of mycotoxins in poultry

Synergistic (red line) and additive (dashed line) effects of mycotoxins in swine

Source: biomin.net

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MEASURING THE COMPLETE TOXICOLOGICAL PATTERN o Rapid tests for mycotoxin analysis are

available only for regulated mycotoxins.

o How can we get a comprehensive

picture of the contamination level of a sample?

Aflatoxins?

Beauvericin?

Deoxynivalenol?

Fusaric acid?

Ochratoxins?

Deoxynivalenol 3-glucoside?

Fumonisins?

Ergots?

Zearalenone?

T-2 and HT-2 Toxin?

Moniliformin?

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MULTI-MYCOTOXIN ANALYSIS LC-MS/MS (liquid chromatography-tandem mass spectrometry) is the state-of-the-art technique for multimycotoxin analysis.

This technique is: o Selective: allows quantification of multiple analytes (up to ca. 400) present in a sample in a single analysis. o Sensitive: the limit of detection for mycotoxins can be in the ppt range.

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MULTI-MYCOTOXIN ANALYSIS 2.6e5 2.4e5 2.2e5 2.0e5 1.8e5 Intensity, cps

1.6e5 1.4e5 1.2e5 1.0e5 8.0e4 6.0e4 4.0e4 2.0e4 0.0

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4

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10 Time, min

12

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MULTI-MYCOTOXIN ANALYSIS 50+ The most comprehensive commercially available multi-mycotoxin method o Method developed and performed in-house by Romer Labs

o More than 50 different mycotoxins and metabolites: o o o o

frequently occurring mycotoxins masked mycotoxins emerging mycotoxins other secondary metabolites

o A key component of BIOMIN’s

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CRITERIA FOR SELECTION OF TOXINS FOR MULTI-MYCOTOXIN ANALYSIS 50+ 端 Legal limits established 端 Recognized masked and emerging mycotoxins 端 Proven scientific information on toxicity available 端 Availability of high quality standards

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There are over 400 known fungal metabolites that may be toxic.

What about the ones that don’t meet these critieria?

MULTIPLE MYCOTOXINS SPECTRUM 380® RESULTS

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MULTIPLE MYCOTOXINS SPECTRUM 380® RESULTS

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POLL QUESTION 3 Over the past 12 months, which mycotoxin mitigation methods have you used? o o o o o

A mycotoxin binder added to feed A multi-strategy mycotoxin deactivation feed additive Good quality control, feed management Mycotoxins are not an issue for us Currently looking for a solution

MYCOFIX® 5TH GENERATION Biotransformation

FUMzyme®

Biomin® BBSH 797

Biomin® MTV Biological constituent

Bioprotection

Adsorption Synergistic blend of minerals WORLD MYCOTOXIN REPORT

Biomin® Bioprotection Mix hosted by

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JUST 3 INGREDIENTS HAVE BEEN AUTHORIZED BY EU AS MYCOTOXIN DEACTIVATORS

Bentonite = Mycofix® Secure

Biomin® BBSH 797 = DSM 11798

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FUMzyme® = DSM 26643 hosted by

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SUCCESS IN FUM AND DON DEGRADATION

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GET THE MYCOFIX® APP FOR IOS AND ANDROID

YOUR DEFINITIVE SOURCE FOR MYCOTOXIN INFORMATION

VISIT BIOMIN.NET

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QUESTIONS & ANSWERS

Alexandro Marchioro, MV, MSc Senior Mycotoxin Expert at BIOMIN WORLD MYCOTOXIN REPORT

Martina Bellasio, Dr. Senior Mycotoxin Expert at Romer Labs hosted by

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DELIVERED TO YOUR INBOX

Sampling guide

Report

On-site testing video

biomin.net romerlabs.com

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