Mycotoxin Occurrence in 2018 US Corn
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Sample Overview
71%
5%
3% 232 Samples from 25 States
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5%
17%
Sample Overview Sample Matrix
Sampling Reason
10%
22% 14%
14%
64%
76%
Corn
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Corn Silage
Corn Byproduct
Survey
Clinical
Unspecified
Mycotoxins & Analysis
Mycotoxin Group
Aflatoxins (AFLA)
A-Trichothecenes (A-Trich)
LC-MS/MS
B-Trichothecenes (B-Trich)
Fumonisins (FUM)
3 Labs
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Mycotoxins
Limit of Detection (ppb)
Aflatoxin B1
1.3
Aflatoxin B2
1.2
Aflatoxin G1
1.1
Aflatoxin G2
1.6
T-2
100.0
HT-2
100.0
Neosolaniol
100.0
Diacetoxyscirpenol (DAS)
100.0
Deoxynivalenol (DON/Vomitoxin)
100.0
Acetyldeoxynivalenol (A-DON)
100.0
Nivalenol (NIV)
100.0
Fusarenon X (FUS-X)
100.0
Fumonisin B1
100.0
Fumonisin B2
100.0
Fumonisin B3
100.0
Zearalenone (ZEN)
Zearalenone (ZEN)
Ocharatoxin A (OTA)
Ocharatoxin A (OTA)
51.7 1.1
Occurrence 100
Percent of Total Samples
80
88 81
60
40 30
54
39 31
65
56
52
45
52
42 27
20
14
10
8
0
67
65
70
50
73
72
57
79
2012
2013
471 24 19
# Samples per year
32
595
318
692
17 5
1 2014
2015 Afla
3
2016 B-Trich
ZEN
2017 FUM
B-Trichothecenes include Deoxynivalenol, Nivalenol, Fusarenon-X, Acetyl-Deoxynivalenol A-Trichothecenes and Ochratoxin A are not presented due to insignificant positive samples
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700
78
600 500 400
38
300
232
200
9
100
2018
0
Total Samples Analyzed/Year
90
800
Mean of Positives 100.000 15.234
ppb (log scale)
10.000 2738 1.000
2.056 218
100
109
3.878
2.093
1438
542
489
2012
339
287
143 51
2013
19
ZEN
2015 B-Trich
2016 FUM
B-Trichothecenes include Deoxynivalenol, Nivalenol, Fusarenon-X, Acetyl-Deoxynivalenol A-Trichothecenes and Ochratoxin A are not presented due to insignificant positive samples
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927
1123
250
274
39
2014 Afla
Š BIOMIN Holding GmbH 2018
3.560
126 20
10
1682
1.190 691
2.225
11 2017
2018
Median of Positives 2.000
1.000
ppb (log scale)
600 200
83
928 430 283 106
1.450
1.400
600
500
933
400
179
107
194
700 486 126
10
10
6
6
2016
2017
4 2
2012
2013
2014 Afla
2015 B-Trich
ZEN
FUM
B-Trichothecenes include Deoxynivalenol, Nivalenol, Fusarenon-X, Acetyl-Deoxynivalenol A-Trichothecenes and Ochratoxin A are not presented due to insignificant positive samples
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144
27
23 20
669
2018
Co-occurrence 77
80
Percent of Total Samples
70 60
60 54
50 40
45 35 29
30 20 10 0
26 11
44 42
13
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47
39
44
15
2012
2013
2014
2015 1 Mycotoxin
34
17 4
<LOD
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46
2016 >1 Mycotoxin
9
2017
6 2018
2018 Co-occurrence 25
100
23
9
21
13
12
13
10 6
5
One toxin
Two Toxins
1 Others
B-Trich + FUM + Afla
B-Trich + ZEN
B-Trich + FUM
Afla + FUM
FUM
B-Trich
Afla
ND <LOD
2
1 B-Trich + FUM + ZEN
2
1
Others
5
Percent of Positive Samples
15
0
31
80
FUM + ZEN
Percent of Total
20
18
> 2 Toxins
B-Trichothecenes include Deoxynivalenol, Nivalenol, Fusarenon-X, Acetyl-Deoxynivalenol A-Trichothecenes and Ochratoxin A are not presented due to insignificant positive samples
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60
40
91
100 82 69
20
0
Afla
B-Trich
FUM
ZEN
Co-occurrence Stand-alone Occurrence
Distribution of Positive Samples 59% of Afla samples over 20 ppb <20
Afla
20-50
51-100
101-300
41%
301-1000
23%
>1000 18%
14%
5%
36% of DON samples over 1000 ppb B-Trich
<150 7%
150-300
301-900
13%
901-1000
1001-2000
45%
5%
>2000
16%
15%
41% of FUM samples over 2000 ppb FUM
<1-750
750-1000
34%
1001-2000 6%
2001-5000
19%
5001-20000 19%
>20000 19%
3%
71% of ZEN samples over 100 ppb <50
ZEN
29%
50-100
101-200 33%
201-500
501-1000
>1000 25%
B-Trichothecenes include Deoxynivalenol, Nivalenol, Fusarenon-X, Acetyl-Deoxynivalenol A-Trichothecenes and Ochratoxin are not presented due to lack of positive samples Naturally ahead Š BIOMIN Holding GmbH 2018
9%
4%
2018 Distribution by State: Afla n=1
n=9 n=25 4%
n=6
n=1 100% n=6
n=2
n=1 n=6 n=2
n=52
n=27 15%
n=11 n=3 9%
n=21 n=4 25%
n=1
n=4 50%
n=2 n=21 29%
n: # of samples submitted/state N%: % of samples tested positive/state Risk to livestock based upon threshold levels according to FDA and EU regulatory and guidance values • • • •
State with average > 20 ppb State with average < 20 ppb State with samples < LOD (1 ppb) No sample submitted Naturally ahead © BIOMIN Holding GmbH 2018
n=1
n=14 21%
n=8 25% n=1
n=2 100%
2018 Distribution by State: B-Trich n=1 100%
n=6 67% n=1 100% n=6 83%
n=2 50%
n=9 100%
n=25 80%
n=52 94%
n=1 100% n=6 100% n=21
n=11 n=3 100% 55% 100% n=1 100%
n=27 30%
n=2 100%
n=4 100% n=4 25%
n=2 n=21
n: # of samples submitted/state N%: % of samples tested positive/state Risk to livestock based upon threshold levels according to FDA and EU regulatory and guidance values • • • •
State with average > 1,000 ppb State with average < 1,000 ppb State with samples < LOD (100 ppb) No sample submitted Naturally ahead © BIOMIN Holding GmbH 2018
n=14 36%
n=8 n=1
n=1 100%
n=2 100%
2018 Distribution by State: FUM n=1
n=6 50% n=1 100% n=6 33%
n=2 100%
n=9 22%
n=25 24%
n=52 94%
n=27 93%
n=2 100% n: # of samples submitted/state N%: % of samples tested positive/state Risk to livestock based upon threshold levels according to FDA and EU regulatory and guidance values • • • •
State with average > 2,000 ppb State with average < 2,000 ppb State with samples < LOD (100 ppb) No sample submitted Naturally ahead © BIOMIN Holding GmbH 2018
n=1 n=6 33% n=3
n=11 100% 55%
n=21 52%
n=1 100%
n=21 100%
n=2 100%
n=4 100% n=4 75%
n=14 100%
n=8 63% n=1
n=1 100% n=2 100%
2018 Distribution by State: ZEN n=1 100%
n=6 33% n=1 100% n=6 17%
n=2
n=9 67%
n=25 24%
n=52 63%
n=27 4%
n=1 n=6 83%
n=11 n=3 45%
n=21 76%
n=2 50%
n=4 50%
n=1
n=4
n=2 n=21
n: # of samples submitted/state N%: % of samples tested positive/state Risk to livestock based upon threshold levels according to FDA and EU regulatory and guidance values • • • •
State with average > 100 ppb State with average < 100 ppb State with samples < LOD (51.7 ppb) No sample submitted Naturally ahead © BIOMIN Holding GmbH 2018
n=14 7%
n=8 n=1
n=1 n=2
2018 Risk Levels - Poultry 10000 3.560 1.123
(ppb) log scale
1000 274 126
Risk levels for broiler, breeder, layer, duck, turkey - based on 100% inclusion rate in diet
100
Low risk Medium risk Medium-High risk High risk
10
1
Exception to risk level for broiler
Afla
ZEN
B-Trich
B-Trichothecenes include Deoxynivalenol, Nivalenol, Fusarenon-X, Acetyl-Deoxynivalenol A-Trichothecenes and Ochratoxin A are not presented due to insignificant positive samples
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FUM
2018 Risk Levels - Swine 10000 3.560 1.123
(ppb) log scale
1000 274 126
Risk levels for nursery, grower, finisher, sow, boar - based on 100% inclusion rate in diet
100
Low risk Medium risk Medium-High risk High risk
10
1
Exception to risk level for grower and finisher
Afla
ZEN
B-Trich
B-Trichothecenes include Deoxynivalenol, Nivalenol, Fusarenon-X, Acetyl-Deoxynivalenol A-Trichothecenes and Ochratoxin A are not presented due to insignificant positive samples
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FUM
2018 Risk Levels - Ruminants 10000 3.560 1.123
(ppb) log scale
1000 274 126
Risk levels for calves, dairy cows, beef cattle - based on 100% inclusion rate in diet
100
Low risk Medium risk Medium-High risk High risk
10
1
Exception to risk level for beef cattle
Afla
ZEN
B-Trich
B-Trichothecenes include Deoxynivalenol, Nivalenol, Fusarenon-X, Acetyl-Deoxynivalenol A-Trichothecenes and Ochratoxin A are not presented due to insignificant positive samples
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FUM