SYNTHESIS AND PRELIMINAR CHARACTERIZATION OF HISTIDINE-METAL COMPLEXES Cayla Guillory, Dr. Gina Chiarella, and Dr. Huajun Fan Department of Chemistry Prairie View A&M University
Abstract In this research, Synthesis Characterization and Computation Modeling of Metal Histidine Complexes, we are to prepare transitional metals complexes using the Amino Acid L- Histidine. Only focusing on 4/38 transition metals and that is Cobalt (ll), Chromium (lll), Copper (l), and Manganese (ll). It is the preparation of transition metal complexes with especial interest in compounds with potential catalytic activity and resemblance with biological molecular structures.
Results
Synthesis of Histidine – Chromium (III) complex
A preliminary analysis of the products was executed by infrared and UV-visible spectroscopy, the respective spectra were studied, indicating that products and reactants presented different peaks, which indicate that the products are different compounds; additional analysis will be required to determine the structure of those compounds.
Infrared spectra in solid state of Histidine and the product Histidine-Metal complexes
Introduction Amino acids binding transition metals are very common in biological systems; they are present in enzymes playing powerful role in metabolic processes, detoxification and preservation of different living entities; however the nature and structure of the Metal-amino acid or metal peptide bond is not totally clear, as well as, the mechanism that allow their formation; this information would be crucial in the prevention of some diseases and in the preparation of new medicines. This project will try to contribute to the knowledge of the L-histidinetransition metal connection, and their chemical and physical properties for posterior uses.
Synthesis of Histidine – Cobalt (II) complex
Goals
UV-vis spectra in aqueous solution of Histidine and the product Histidine-Metal complexes
My research has two main objectives: •To synthesize the metal complexes of the Amino Acid L-Histidine and the metals Chromium (lll), Copper (l), Manganese (ll),and Cobalt (ll). •To perform the instrument analysis to characterize those examples and make the computation calculations of the structure
Experimental procedure
Synthesis of Histidine – Manganese (II) complex
Preparation of Cobalt(II) Histidine Complex: Aqueous solution of L-histidine is mixed with aqueous solution of Cobalt(II) nitrate in a 100-mL beaker in mole ratio of 2:1, the mixture was stirred for half an hour until the solution change from pink-reddish to a very dark orange, latter the product was evaporated and analyzed. Preparation of Manganese (ll) Histidine: Aqueous solution of L-histidine is mixed with aqueous solution of Manganese(II) sulfate in a 100-mL beaker in mole ratio of 2:1, the mixture was stirred for half an hour until the appearance change from cloudy withe to clear colorless, latter the product was evaporated and analyzed. Preparation of Chromium (lll) Histidine: Aqueous solution of L-histidine is mixed with aqueous solution of Chromium(III) nitrate in a 100-mL beaker in mole ratio of 2:1, the mixture was stirred for half an hour until the solution change from dark green to a creamy light green, latter the product was evaporated and analyzed. Preparation of Copper (ll) Histidine: Aqueous solution of L-histidine is mixed with aqueous solution of Copper(II) nitrate in a 100-mL beaker in mole ratio of 2:1, the mixture was stirred for half an hour until the solution change from bright green to a dark blue color, latter the product was evaporated and analyzed
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POSTERS AND REPORTS
Summary The metals Copper (ll) Histidine, Chromium (lll) Histidine, Manganese(ll) Histidine, and Cobalt (ll) Histidine complexes has been synthesized.
Synthesis of Histidine – Copper (II) complex
References •Richard J. Sundberg and R. Bruce Martin. Chemical Reviews, 1974, 74, 471517 •Georgia C. Boles, Rebecca A. Coates, Giel Berden, Jos Oomens, and P. B. Armentrout. J. Phys. Chem. B, 2016, 120, 12486−12500 •Lei Zhou, Shenhui Li, Yongchao Su, Xianfeng Yi, Anmin Zheng, and Feng Deng. J. Phys. Chem. B, 2013, 117, 8954−8965
•Acknowledgements R&I’s Office of Undergraduate Research (OUR and Chemistry Department,, Prairie View A&M University.
