Injection of beta amyloid brain extract intravenously is shown to induce Alzheimer’s Like Disease in APPSwe/PS1dE9 mice –A Critical Review Nikol Digtyar
Alzheimer’s Disease is one of the most common neurodegenerative diseases. There has been much significant research in the field to determine whether Alzheimer’s Disease could be a transmissible disease. The spread of Alzheimer’s disease has been linked to seeding of β-amyloid protein plaques in the brain. However, the studies which were completed injected Human Alzheimer’s disease brain extracts intracerebrally into the hypothalamus and other corresponding brain regions. The following study was completed on transgenic APPSwe/PS1dE9 mice. There were three groups in the experiment. A control group was injected with brain extracts from a healthy male adult (HTC), the experimental groups were injected with brain extracts from two male human Alzheimer’s Disease patients (AD1, AD2). Both experimental groups were additionally subdivided. One subdivision was injected with the brain extract intracerebrally and the other was injected intravenously. Mice were then sacrificed 180, 270, and 360 days post-injection. The brain was then extracted and placed in formaldehyde and embedded in wax. Several histological analyses were completed on the tissue samples. β -amyloid was detected using anti-Abeta 4G8 antibody. The results of this study demonstrate that 180 days post intravenous injection of AD1 brain extract, mice began to develop β-amyloid plaques in the vasculature of the thalamus. This suggests that the β-amyloid brain extract was able to bypass the blood-brain barrier. The intracerebrally injected mice also showed similar results 360 days post injection.
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