KEHAVÄLISEL VILJASTAMISEL KASUTATAVA MATERJALI MIKROBIOLOOGILINE KONTROLL
Determination of bacteria in the in vitro fertilization materials
Juliana Baranova, Ülle Parm, Jelena Štšepetova, Reet Mändar
Abstract Over the past few years, a lot of studies have been carried out in the field of in vitro fertilization (IVF). Some of these studies have indicated the presence of high variety bacteria in the IVF materials. However, no similar studies have been conducted in Estonia. The aims of this study were to determine the prevalence and counts of bacteria in the IVF materials, connections between them, clinical patient data and procedure outcome. Studied samples (n=50) of infertile couples included: collected semen, treated semen, incubated semen and fertilization solutions 1 and 2. Collected samples were quantified by real time PCR for total counts and three groups of bacteria: Enterobacteriaceae, Corynebacterium spp. and Staphylococcus spp. Descriptive statistics, t-, Mann-Whitney, χ2-tests and Spearman’s correlation were used for the comparison of studied groups. Statistically significant difference was considered by p <0.05. The prevalence and counts of bacteria in the IVF materials were lower in the semen after incubation compared to the semen after collection and treatment. Unexpectedly, the prevalence and counts of total bacteria and Corynebacterium spp. were higher in fertilization solution 2 than in solution 1, although the same treated semen was used for fertilization and Corynebacterium spp. count reduced with treatment remarkably. The count of Enterobacteriaceae was higher in solution 1 in comparison to solution 2. The positive correlation 61
